LOCUS       DASSHJ010000001        10855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_652699, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000001 DASSHJ010000000
VERSION     DASSHJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652699"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFI43_00005"
     CDS             <1..387
                     /locus_tag="VFI43_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7060538.1"
                     /translation="ANARTAITFALSPGQAHDAPEGRALLNRLGPTSYPLYLVMDRAY
                     EGAETRQLAFDLGYVPVVPPKSSRIHAWEYDREMYKRRNEIERLFRRLKGFRRIFSRF
                     EKLDVIFIGFIHFALIVEAFRSVNTL"
     gene            459..656
                     /locus_tag="VFI43_00010"
     CDS             459..656
                     /locus_tag="VFI43_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060539.1"
                     /translation="MPKSIWEKAGNEARERAEQAKDFAVEANADIDREADSLLEKAKG
                     SRFTGMIALGVIVLILLAVVF"
     gene            684..995
                     /locus_tag="VFI43_00015"
     CDS             684..995
                     /locus_tag="VFI43_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060540.1"
                     /translation="MRSRVETIEQMQKKHEERMAILELGHERHEEMQVKQAETLDRMS
                     LNVQRMADGMTHLVSIVPLINMAQAMNMICKWLAPPFAIVAGLWAAGHTIYLLLENSG
                     G"
     gene            1078..1392
                     /locus_tag="VFI43_00020"
     CDS             1078..1392
                     /locus_tag="VFI43_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060541.1"
                     /translation="MTGIASIAEVSQRELIVTANAGKTLSASSEQGSETACKPKEASR
                     TLRFNKWVGYAAASVSGLVSLGDAFGYMKADWTCAFLLIAVVAISSYNYWLRLHTSRP
                     VK"
     gene            1676..2164
                     /locus_tag="VFI43_00025"
     CDS             1676..2164
                     /locus_tag="VFI43_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HET7060542.1"
                     /translation="MAGKRRATAVFALSASAFVALAMSEGYRSTAYIPVPGDVPTIGF
                     GTTGGVRLGDTITPPRALSRALSDIQKFEGALKKCVIVPLHQHEYDAFVRLAYNIGAS
                     AFCNSTLVKLLNQGRYAEACEQIKHWNRQGKKILPGLAKRRQAEYDQCIGKTSDLAEN
                     EG"
     gene            2392..4425
                     /locus_tag="VFI43_00030"
     CDS             2392..4425
                     /locus_tag="VFI43_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060543.1"
                     /translation="MTAFRISGFSGLVPRVAKQLLAPNQAQVAVNCNLTSGDLRPRNG
                     LRLIHAPTIDNDIVSMFRMEKDGNEKWLAWDKDVDVALSPVAGHASGRFYYTGDGEPR
                     VSDFDTSTAGVGPYPSGCYVLGVAPPIAGAVVTPGGGISSTVVSRAYVYTLVTPWGEE
                     SAPSPASTVITGKMDATWRLTGMDGAPPNDGTVIGAASDMPSPGYVTITLDSVFGLRA
                     GEEIRFSSVGGMPDLNTRFLLVSVDASTHQVVVSLATTQTYVSGGAWTRKALHNTQGM
                     IKRIYRTLTSPSGTAYHYVATVTAGAIAFDDTTPDAELGEQLPSAEWLMPPPDMRGIT
                     ILANGIACGFRENEVCFSEAFKPYAWPAAYRQTYDQEIVAIGAMGTTLVGMTKGNPFT
                     ITGVEPATMGGGMDKLSVAWPCMSKRGVANFGFGVGYPAPQGMVIIGGESDIVTRNLF
                     TQKEWSALNPETFIAASADNRYYCGYSASGSSLMFVIDKAEAAAFIQINQKITAIWSD
                     PQTGRLYVAADRKIHEWEGDTGTKLAYEWKSKKFVTAPPVNYGAAKIDADFEMTEAES
                     AAAQVAHDATIMANAGLMAGNSMNDGLGDACLGEFEISGDEMRPVPPLAIDSLQFQLW
                     ADGALKYARQVRNDRAFRLPGGYKSDNVEIVLSGNVKVSGLVLAETMDGLKRA"
     gene            4531..4914
                     /locus_tag="VFI43_00035"
     CDS             4531..4914
                     /locus_tag="VFI43_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060544.1"
                     /translation="MAKYAHADVLDGGLNAIRNNAIRMLLLKNYTVADSYATVTGNAI
                     CTVVMVSADYTLSGADGASRILTIAAKSGTASANSGISPNLHIAFTDNVGKVLLVTDE
                     TTDQVVTSGNTVNFPSLTYTSGQPV"
     gene            4959..5396
                     /locus_tag="VFI43_00040"
     CDS             4959..5396
                     /locus_tag="VFI43_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060545.1"
                     /translation="MPAFTQAQASRSASLLNLSSLANAAYTASPAIDLGAAIPLDVTL
                     EVECVPGAATTGNKQLILFAKFSLDNTNFGSGPENGSSTTNESDLHWIGTLPCNDSAS
                     HRKFFNLQGLPVTRYLKLVVKNETGVALASGNVYRADITGASA"
     gene            5400..7055
                     /locus_tag="VFI43_00045"
     CDS             5400..7055
                     /locus_tag="VFI43_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET7060546.1"
                     /translation="MSSLILPARFRQQPQSAPRLAPAYQDAELLAPCIDGTGSDIARA
                     NHLLPGTAPARTIRQHGYALNFNGSSHYLVSARAVKPVGQFSVFAWVRPAAVNTSYTR
                     IIETDYTSGFYLGSNTASRYCWIVNNASLEGCIGGQQVAGQRDFICGVFDGSNRILYV
                     NGIQAASSAATAPSAALPVYISRWNNTPGYYWNGDIDTVAIFNRALGASEIWSLYQNP
                     WQLFKGPARRLWSAPGTHNLAAGGPIQANAAAAAEITLVQALGGIPVFQFNAIASAAV
                     TQSHTLATNDGVAGEGSVQGNAAATAEITQAHALAAVPSVQLTASTAAGVTQDHALAS
                     AGVEAQSNTGATGAIVQIHTLAPDDATQPGSASAAGIVIALALSTGPWSQTNAALIGQ
                     IAQAHTLAMLPALTQLNSSTVITVTQAHALVVANAAQANIGSSTAIIPGTGNLAAASL
                     LQTNICGSAAIALAGAIPDVFRNSIAASAAIKKPGIPGGTPEWLRTFLEIISGRRGNR
                     IAIPPLEILTFSATPTKSECEALYSYVNKVRASMADLINRLDG"
     gene            7056..7667
                     /locus_tag="VFI43_00050"
     CDS             7056..7667
                     /locus_tag="VFI43_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7060547.1"
                     /translation="MNNDLISLLRANLGAVLSAELAADICMAASAVPRLIPAEAIDGL
                     RSREHDGFAFGVERIEDIAAEMKPLHRAHWNETESHRHGLELNPDYEAFTRYERSGRY
                     LLFTLRKDGRLLGNCAIYLDRSAHTQTLIATEDTLYLLPEARRGRVARRFVAYVEDAL
                     RELGVREIHITVKTVNRAGRFLQMLGYRHVENGLMKILEVDNV"
     gene            7660..8907
                     /locus_tag="VFI43_00055"
     CDS             7660..8907
                     /locus_tag="VFI43_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060548.1"
                     /translation="MCSPKPPKPDPLIGQAAKQNADIAQQQLDAAREQLAWEKDRART
                     QDPLIQKIVDQQIQSGNTNAARAESQWETYRKLFAPLEEQMVYEAADFDSVERKDRAA
                     AEAAADVRRGYQGALEANERAMERMGVNPDSGKFQAAMKDINLSLARDSAGAMNKARR
                     DTELQGMAMRQGAAQFGRNMPTTGIASDAAALSAGNAATDNLATKAGLHNAALNAAQN
                     WFGGAASANTAAGNLGLGQYQGQLNAWQQASQNSASNAAGLGSLFGQLGSAYLMKPVA
                     LRSGGIIKPGEPVNQAVPPGADRNADLSRFPLQNRGSSGLSFLKRSGRDGGGLIEGPG
                     TGASDSIPATIEGIQPVRLSNGEAVLNAEAVQLLGEDFIHRVNNAGLGGAETGARRRG
                     RGEQSGQKPVQGHGKTGGRKANA"
     gene            8900..9673
                     /locus_tag="VFI43_00060"
     CDS             8900..9673
                     /locus_tag="VFI43_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060549.1"
                     /translation="MLNVGAFAGGLAQGIRSGQEMQVKAQTADRLARTGDQETEMHQF
                     RMDKAAFEKDKRNRLKAANEEIAAGWEEVGQGSADESPASVTPGLKDLASPEGRRGET
                     VPAGLASESPAAGLSQLGKLPAPRAQAMQRPDPAEAIGRRMLVGDLLENKDELTRMAN
                     IYKKHGLLADMLPWMNSAYSAKKKRIPDALHLLLAVDAKGAREALRQGGINLTDDPVQ
                     VNPGNGRAGMWKFRFEGNSEKEIGLRELARKFIPYIRNL"
     gene            9911..10090
                     /locus_tag="VFI43_00065"
     CDS             9911..10090
                     /locus_tag="VFI43_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060550.1"
                     /translation="MAVLDVVLPQACRGVARVGYNVGNWRVDKHSRFVEDITGDGTAD
                     IVRFSNSGTWVSEWF"
     gene            complement(10269..>10855)
                     /locus_tag="VFI43_00070"
     CDS             complement(10269..>10855)
                     /locus_tag="VFI43_00070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7060551.1"
                     /translation="KLFYYARPGTPRNGFEIAVTALQQLKQEFGDSLDIVCAGADWNP
                     ADYGLDGAVRNIGLLPYEETGEIYRSVHVGLVMMMTKHPSYLPLELMASGAVVVSNYN
                     PATTWLLKNGENCLLSAPTASCLAATLRYALDNYEQLASLRKRAAFQILADAGDWETS
                     LGEVASFILKPPNQSVQSNSAVRALLPTVGTLGR"
BASE COUNT         2563 a         2835 c         3214 g         2243 t
ORIGIN      
        1 gcgaatgctc gaacggctat aacctttgca ttgtccccag gtcaggcaca cgatgcccct
       61 gaaggccggg ctctgctcaa tcggcttggc ccaacaagct atcctcttta tcttgtgatg
      121 gatcgcgcct atgaaggtgc cgaaacccgc caactcgcgt tcgatcttgg ttatgtgcct
      181 gtcgtcccac cgaagagcag tcgaatccat gcctgggaat acgatcgaga gatgtacaag
      241 cgccgcaatg aaatcgagcg cctgtttcgc aggctcaagg gctttcgacg aatattctcg
      301 cgtttcgaaa aactcgatgt cattttcatc ggatttattc atttcgctct gatcgtcgaa
      361 gcattccgta gtgttaacac actctagttg ctgtgcgagt ttaacaagag tagttagcgc
      421 cgcccctccg ggcggttttt tatttaagga gacaagaaat gccgaaatca atatgggaaa
      481 aagcgggcaa cgaggcacgg gaaagagcgg agcaggcaaa ggatttcgcg gtggaagcaa
      541 atgccgatat cgatagggag gccgactcgc ttctcgaaaa ggcgaagggc tcccggttca
      601 ccggaatgat tgcgctagga gttatcgtac tgatcctgtt ggcggtcgtg ttctagggcg
      661 aagcggtgaa acaagagtgc ccgatgcgct cccgcgtgga gacgattgag caaatgcaga
      721 agaagcacga agaacgtatg gcgatattgg agttggggca cgaacggcac gaggagatgc
      781 aggtaaagca agcggaaacc ctcgacagga tgtctctcaa tgtccagcga atggcggacg
      841 gcatgaccca cctggtatcc atcgttccct tgatcaatat ggcgcaggcc atgaacatga
      901 tctgtaagtg gcttgcccca ccgtttgcga tcgtcgccgg gctgtgggct gcgggacaca
      961 caatctacct gctgttggaa aattcgggag gctgaatgga tgattctgat agggcatccg
     1021 agcttgagga aatgtggagg caggcggatc tcggcaggag aaaaccggag ccgaaacttg
     1081 acgggcattg cctcaattgc ggaagtgagt cagagggagc ttattgtgac agcgaatgca
     1141 gggaagacgc tgagcgcgtc gagcgagcaa ggatcagaaa cggcgtgcaa gccgaaagag
     1201 gcgtcgcgca cacttagatt caacaagtgg gtgggatacg cggccgcaag cgtatcgggg
     1261 ctcgtgagtc tcggtgatgc atttggatac atgaaggcgg actggacgtg cgcgttcctg
     1321 ctcatcgcgg tcgtcgcaat cagcagctac aactactggc ttcgcctgca tacgagcagg
     1381 cctgtgaaat gagctggaat aaaaggtaat gaaaggaaat gaaaaaactg aagggttggc
     1441 cggtaacgta cacggggatt gcgtcgcttt cggcaggttc gtggatcaat agcggctctg
     1501 acgcagggct cattacgtgc ggcctctgtt tgattgtctc gggtctgata cagcttgcga
     1561 ggtcttgata cccaaatatc tctcagctgt tgctcatctt tgaacggcgg gtttccgccg
     1621 cgcattgccg gcaacagcga tgggaggtaa aagaacaaaa gggaaaaagg gaaaaatggc
     1681 gggaaaacga agggcaacag cagtttttgc attatcggcc tccgcatttg tcgccctcgc
     1741 catgagcgaa ggttatcgca gcaccgcgta tattcccgtc ccgggcgacg tgcccaccat
     1801 cggcttcggt actacagggg gggtgcgcct gggggacacg attactcccc ccagagcgtt
     1861 gtcgcgcgcc ctatccgata ttcagaagtt cgagggggca ctgaaaaaat gcgttatcgt
     1921 gcccctgcat cagcacgagt atgacgcctt cgtacgtctg gcttataaca tcggggcgtc
     1981 ggccttctgt aattcgactc tggtcaagtt gctgaatcag ggacggtacg ctgaggcctg
     2041 cgaacaaatc aagcactgga accggcaggg gaagaaaata ttaccgggct tggccaagcg
     2101 ccgacaggct gaatacgacc aatgcatcgg aaagaccagt gacttggctg aaaacgaggg
     2161 ataacgggtg gtggtgaggc attgcctttt tgagatagac gtttctcttc cctggaacgc
     2221 cccaacgagg aacctcatca agccgccttc ttctgcccct tcccagaagc aggggagggg
     2281 aatttactag cagggcctta gtactcaggt tcagggctat aaagcacacg catgtgaact
     2341 tatggtttat tcatcagaca tttaaagttt ggcatcgcac ctcctatttt tatgactgcc
     2401 tttcgtatat caggattttc cgggcttgtt cccagggtcg ccaagcaatt gctggcgccc
     2461 aatcaggccc aggtagcggt caactgcaat ttgacgagcg gggatcttag gccgagaaac
     2521 gggctgaggc tgatccatgc accaaccatc gataatgaca ttgtctcgat gtttcgaatg
     2581 gagaaagacg gcaatgaaaa atggctggca tgggataagg acgtcgatgt ggcgctatcc
     2641 cccgttgccg gccatgcctc cggccgcttc tactacacgg gtgatggaga gccgcgcgta
     2701 tcggacttcg acacatcaac ggcgggcgta ggtccttatc cttcaggatg ttatgtcctg
     2761 ggggttgcgc ctcccatagc cggagcggta gtgacaccgg ggggtggaat tagctcaaca
     2821 gtggtatcga gagcttacgt ttataccttg gttaccccct ggggtgaaga atccgccccg
     2881 tccccggctt caacggtgat aactggaaag atggacgcaa catggaggct gacagggatg
     2941 gatggggcgc ctcctaatga tggaacggtg attggggccg cgagcgatat gccctcgcca
     3001 gggtatgtga cgatcacgct ggactcggtg tttgggcttc gtgctggaga ggaaatacga
     3061 ttttcctcgg tgggtggaat gcctgatttg aacacacggt tcttgctcgt gagtgtcgat
     3121 gcctctaccc atcaggttgt ggtatcgctc gcgacaaccc agacatacgt atctgggggg
     3181 gcttggacgc gaaaggcgct tcataatacc caaggcatga tcaagcgtat ttatcgaacg
     3241 ttgaccagtc cttcgggaac agcctatcac tatgttgcga ccgttaccgc gggcgctatc
     3301 gcattcgacg acactactcc tgacgccgag cttggggagc agttgccgtc cgccgaatgg
     3361 ctgatgcccc cgccggatat gaggggtatc acgatcctgg cgaacggcat tgcctgcggt
     3421 ttccgcgaaa acgaagtgtg cttctcggaa gcattcaagc cttatgcctg gccggctgcc
     3481 taccgccaga cgtacgacca ggagatcgtc gctattggcg cgatgggcac gacacttgtc
     3541 ggaatgacaa aaggaaaccc tttcaccatc acaggagtgg agcccgcgac gatgggcgga
     3601 gggatggaca agctgagcgt ggcctggccc tgcatgtcaa agcgtggagt cgcgaatttt
     3661 ggctttgggg tcggctatcc tgcgccgcag ggcatggtca ttatcggcgg ggaaagtgac
     3721 atcgtgacca ggaacctgtt cacgcaaaaa gagtggtccg cgctcaatcc ggaaacattt
     3781 atagctgctt cggctgacaa ccgctattac tgcggctact cagccagcgg aagctcgctc
     3841 atgttcgtga tcgacaaggc ggaagcggcg gcgtttatcc agatcaacca gaagatcacc
     3901 gctatatggt cggaccccca gacagggagg ttgtacgtcg cggcagacag gaaaatacac
     3961 gagtgggaag gcgatacggg gacgaaactc gcctacgagt ggaagagcaa gaaattcgtg
     4021 actgcgccgc cagtgaatta cggcgccgca aaaatcgacg ccgactttga aatgactgaa
     4081 gccgagtcgg ctgcagcgca agttgcccat gatgcaacca ttatggcaaa tgcggggcta
     4141 atggccggca acagcatgaa cgacggattg ggggacgcgt gccttgggga attcgagatt
     4201 agcggcgacg aaatgcggcc tgttccaccg cttgcgattg attcactgca attccagctt
     4261 tgggcagatg gcgccctgaa atacgcgagg caggtgcgta acgatcgcgc tttccggcta
     4321 cccggcggtt acaagtcaga caatgtggaa atcgtgttat caggcaatgt gaaagtttcg
     4381 ggcctggtgc tggccgagac catggacggg ctgaagaggg cataacaacc taagtgaggt
     4441 cagccagggg atagcaggaa gcccattgga agtaccacga agcgggccgc ttgggagcgg
     4501 ctttttttat taccatgaaa ggagcaataa atggcgaaat acgcccacgc agacgttctg
     4561 gatggcggac tgaacgcgat caggaacaac gccatccgca tgctgttact caagaattat
     4621 accgttgcgg acagttacgc gaccgttacc ggtaatgcga tttgcacagt tgtgatggtt
     4681 tccgcggact acaccttgtc aggtgcggac ggcgcatcga ggatacttac aatcgctgcc
     4741 aagagcggga cggcttccgc aaactcgggt atttctccca acctgcacat tgcattcacg
     4801 gacaacgtgg gcaaggtatt gctggtaacg gacgagacca ctgatcaggt ggttaccagc
     4861 ggcaatactg taaattttcc cagcctgacg tatacgagcg gccagccggt ctgatcacgc
     4921 atcgcttaca ggtatccaag tcatcaggag gatgaataat gcccgctttt acacaagccc
     4981 aagcctctcg cagcgcatcc ctgctcaacc tcagctcgct cgcaaacgcg gcgtatactg
     5041 cttcgcctgc catcgatctc ggcgccgcca ttcctctgga tgtgacactg gaggtggaat
     5101 gcgtgccggg cgcagcgacg accgggaaca agcaactcat cctttttgcg aagttttccc
     5161 tggacaacac aaatttcggc agcggaccgg aaaatggcag ttccacgaca aatgagtccg
     5221 atcttcactg gatcggtacg ctgccctgca acgacagtgc gtcccacagg aaattcttca
     5281 atttgcaagg tctgccagtt acccggtatc tcaagctcgt ggtgaaaaac gaaacagggg
     5341 ttgcgcttgc ttcagggaac gtctatcgag cggatatcac gggcgcgtcg gcttaggcca
     5401 tgtcttcttt aattttgcca gcgaggttca ggcagcaacc ccagtcggcc cccaggctcg
     5461 ctcccgcgta tcaggatgct gaactgctcg ccccgtgcat cgacggcaca gggagtgaca
     5521 tagccagggc aaaccatttg ctacccggga ctgccccagc ccgaaccata cgccagcacg
     5581 gctacgcgtt gaacttcaac ggatcgagcc actaccttgt cagcgcgcgt gcggtaaagc
     5641 ctgtggggca gttcagcgta tttgcgtggg taagacctgc ggctgtcaac acctcttata
     5701 cccggataat cgaaaccgat tacacgtcgg gtttctatct cggctcaaac acagcgtcac
     5761 gttattgctg gatcgtcaac aacgcatcgt tggaagggtg cattggaggc cagcaggtcg
     5821 ctggccagag agattttatt tgcggtgtat tcgatggctc gaaccgcatt ttatatgtca
     5881 acggtatcca ggcggcgagc agcgctgcca cggcgccatc cgcggcgctg ccagtctata
     5941 tcagcaggtg gaataatacg cctggttatt actggaacgg tgacatagac actgtcgcca
     6001 tttttaacag ggcgcttggg gcatcggaga tctggtcctt gtaccagaat ccctggcagt
     6061 tattcaaggg gcctgcgcgg cggctatgga gtgcaccggg cacccacaat ctggccgccg
     6121 ggggaccaat tcaggcgaat gcagcagccg cggcagagat cactctggtg caagctctcg
     6181 gcggcattcc ggtattccaa tttaatgcta tcgcgtcggc agcagtcacc caaagtcaca
     6241 ccctcgcgac aaatgacggg gtggcgggag aaggctcggt tcagggaaat gcagctgcaa
     6301 cggcagagat cacccaggca cacgctctcg ccgccgttcc gtcagtccag cttaccgcca
     6361 gcacggctgc aggcgtaacg caagatcatg cgctcgcttc agctggcgtt gaggctcagt
     6421 cgaacaccgg cgcgacggga gcaatcgtgc agatccacac tcttgcaccc gatgacgcga
     6481 cgcaaccggg cagtgcaagt gccgcaggga tcgtgattgc gcttgcgcta tccactggac
     6541 cttggagcca gacgaatgcg gcgttgatcg ggcagattgc ccaggcacat accctggcga
     6601 tgctgccagc cctgacgcag ctcaattcat cgactgtaat cacggtaacg caggcacatg
     6661 cgctggtagt tgcaaatgca gcacaagcaa atattggcag cagtacagca ataatccctg
     6721 gcacgggcaa cctcgcggcc gcttccctgc ttcaaaccaa catttgcggt tcggccgcga
     6781 ttgcgttggc cggcgcgata ccggacgtct tccggaactc gatcgctgcc tccgctgcaa
     6841 tcaagaaacc gggtatacca gggggaacac cggaatggct tagaaccttt cttgagatca
     6901 tttcgggccg acgtggaaac aggatcgcca ttccccccct ggagatactg accttctccg
     6961 ctactccaac caaatccgag tgcgaggcgc tgtattccta tgtaaacaag gtacgagctt
     7021 ccatggctga tcttataaac agactggatg gataagtgaa caatgatctg atcagccttc
     7081 ttcgggcaaa tcttggcgct gttttgagcg ctgaactcgc agctgatatt tgcatggctg
     7141 caagcgcggt cccgcgtctc atccctgccg aagcaatcga cggccttaga agcagggagc
     7201 atgatggctt cgccttcgga gttgaacgga ttgaagatat tgccgcggaa atgaagccat
     7261 tgcatcgagc gcactggaat gagacggaat cgcaccgcca tggcctggaa ctgaatcctg
     7321 actacgaagc attcactcgc tacgagcgca gcggcaggta cttgttattc acgctgagga
     7381 aggacggaag attgctgggc aattgcgcca tttacctcga cagaagcgcc catacccaga
     7441 cactcattgc gaccgaagat accttatatc ttttgcccga agccaggcga ggcagagttg
     7501 cgcgccgatt cgtggcctac gtcgaggatg cgctgcgcga actcggcgtg agggaaatcc
     7561 atattacggt gaagacggtc aacagggctg gccggttcct gcagatgctt ggctatcgcc
     7621 acgtggaaaa cggattaatg aaaatactgg aggttgataa tgtgtagtcc aaagccaccc
     7681 aagcccgatc cactcatcgg gcaggcagca aagcagaacg cggacatcgc ccagcagcaa
     7741 ctcgatgctg caagggaaca actggcgtgg gaaaaggacc gggcgagaac tcaggaccca
     7801 ctcattcaga aaatcgtcga tcagcagata cagtcaggga acaccaacgc ggcgagagcc
     7861 gaatcgcagt gggagactta ccggaagcta tttgcgccgc tcgaagagca gatggtttac
     7921 gaagctgccg actttgattc ggtggagcgc aaggatcgcg ctgccgccga agccgcagcc
     7981 gacgtgaggc gcggctacca gggtgctcta gaagcaaacg agcgagcgat ggagcgcatg
     8041 ggcgtgaacc ccgactccgg aaaatttcag gcggccatga aagacatcaa cctgagcctt
     8101 gcaagggact cggcgggcgc catgaacaag gcgcgcaggg acacggaatt gcaggggatg
     8161 gcgatgaggc agggtgcggc ccagtttggg cgaaacatgc cgaccaccgg cattgccagc
     8221 gatgcagcgg ccttgagtgc cgggaatgcc gcaacggata atcttgccac caaggcaggt
     8281 cttcataatg ctgccttgaa cgcagcccaa aactggttcg gaggcgccgc aagcgctaat
     8341 actgcggccg ggaatctggg tcttgggcaa taccaggggc aactgaacgc ctggcagcag
     8401 gcaagccaga actccgcttc caatgcagca ggactgggca gcctgttcgg gcagctcggc
     8461 agcgcatacc tcatgaagcc cgttgcgttg cgcagtggcg ggattatcaa gccaggggaa
     8521 ccagttaacc aggccgtgcc cccaggagct gatcgaaacg cggatttgtc ccgctttccc
     8581 cttcaaaacc ggggcagtag cgggttatcg tttctgaagc gctcagggcg cgatggaggc
     8641 ggcttgattg aagggccggg aacaggcgcg agcgattcca ttcccgccac tattgaaggc
     8701 attcagcctg tccggttatc aaacggcgaa gcggtgctca acgccgaggc agtgcaattg
     8761 ctgggagaag actttatcca tcgggtcaac aacgccggtc ttggcggcgc tgaaactggc
     8821 gctcggagac ggggcagagg tgagcagagc gggcagaagc cagttcaagg acacgggaaa
     8881 acgggaggga gaaaagctaa tgcttaatgt tggagcattc gcgggtgggc tcgcacaagg
     8941 catacgcagc ggccaggaaa tgcaagtaaa ggcgcagacg gctgatcgac tggcgcggac
     9001 cggcgatcag gaaaccgaaa tgcatcagtt cagaatggat aaggccgctt tcgaaaaaga
     9061 caagcggaac cggctcaaag ctgcgaatga agaaattgca gcaggatggg aagaggtggg
     9121 gcaagggagc gccgatgaat ccccagcatc cgttacaccg gggttgaagg acctggcttc
     9181 cccggaaggg cgacgcggag aaactgtccc ggctggactt gcaagcgaat ctcccgccgc
     9241 cggactgagc caattgggga aattgccggc gccgagggcg caggcaatgc aacgtccgga
     9301 tccggcagag gcaatcggga ggcgaatgct agttggcgac ctgctggaaa acaaggacga
     9361 gcttacccgc atggccaata tttacaaaaa gcatggcctc cttgccgaca tgctcccctg
     9421 gatgaacagt gcttacagcg caaagaaaaa acggattccc gacgcgctgc atctgctatt
     9481 ggccgttgac gcgaaagggg cgagagaagc gctgcggcaa ggcgggataa acctgactga
     9541 tgacccggtt caagtaaatc caggcaacgg acgtgccgga atgtggaagt tcaggtttga
     9601 aggtaattca gagaaggaga ttggtttgag ggagcttgcg cggaagttta ttccttatat
     9661 tcgtaacttg taatagctca gcttaatctg acgtttgttt cggtcttgcc ctatcctcgt
     9721 agattccttt ggaatggcgt tttcagcctg agcccgtgca tgatcgccag ctggaagatt
     9781 ttggcctgtt caagtgcgat gcctactggg aggcgacacc ccttccgagt cttgagtttg
     9841 ctggccaaat aatgcttaat gttcttggcc tcgtatgcag tgcgcttgtc atctcgtcga
     9901 gtagctcgta gtggcggttc tcgatgtcgt actcccacaa gcctgcaggg gggtcgcgcg
     9961 cgtgggttat aacgtaggaa actggcgtgt cgacaagcac tcgcgcttcg tggaggatat
    10021 tactggcgac ggtacagccg atattgttcg cttctcgaac tcagggacct gggtttcgga
    10081 gtggttctga ttttccagga agcgtttttc acgccgttcg gcgctgggta tcatgatgga
    10141 cctcgcgagg aggggtaacg ctccacagcc cctcttgcac cttggcaata ttgattttgg
    10201 ccaaaagaca tcgccgacag ggttggcgaa tagtccagcg tttaagatga gctcccagaa
    10261 gaagtagctt atcttcctaa cgttcccact gtgggtaata atgctctgac tgctgagttt
    10321 gattgaacgg attgattggg aggtttgaga ataaaacttg caacttcacc aaggctcgtt
    10381 tcccagtcac ccgcgtcagc taaaatttgg aaagccgcgc gcttccgaag cgaagccaac
    10441 tgttcataat tgtcaagggc gtatcgcaat gtggccgcca ggcacgaagc cgtaggcgcc
    10501 gaaagcagac agttttcacc attcttgagg agccaggtcg ttgccggatt gtagttgctg
    10561 accaccactg cgcctgaagc catcaactca agaggcaggt aggatggatg cttggtcatc
    10621 atcattacaa ggccaacgtg tacgctccgg taaatctctc ccgtttcctc ataaggcaac
    10681 aagcctatgt ttctgactgc tccatcgagt ccatagtctg ccggattcca gtccgctcca
    10741 gcacaaacaa tgtcgagtga gtcaccgaat tcctgcttta attgctgcag agctgtaact
    10801 gctatttcga atccgttcct cggcgttcct ggtctcgcat aataaaacag ctttt
//
LOCUS       DASSHJ010000002        17160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_978977, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000002 DASSHJ010000000
VERSION     DASSHJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 17160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17160
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978977"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VFI43_00075"
     CDS             complement(<1..131)
                     /locus_tag="VFI43_00075"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HET7060552.1"
                     /translation="MTKAGEWFLCQQPVEINAGRRTLKLKVRNTGDRPIQVGSHYHF"
     gene            complement(225..527)
                     /locus_tag="VFI43_00080"
     CDS             complement(225..527)
                     /locus_tag="VFI43_00080"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HET7060553.1"
                     /translation="MMLTPRETEKLVIYQVADLARRRKDRGLKLNYAEAIALICEALL
                     EGARDGKTLSEVTAIGKQVLKRDDVMDGVPEMVNLIQVEATFLTGTQLITVPDPIS"
     gene            complement(544..774)
                     /locus_tag="VFI43_00085"
     CDS             complement(544..774)
                     /locus_tag="VFI43_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060554.1"
                     /translation="MAWAVGILSAKKLFVGLQIGKKGLQINEHRMQIAFSGDLPMRYA
                     LLPLDDLKLQAARYDSLLVTDCAWIFWGVRHA"
     gene            1100..1531
                     /locus_tag="VFI43_00090"
     CDS             1100..1531
                     /locus_tag="VFI43_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7060555.1"
                     /translation="MAIARYEPWSLLHQLHRELDRAREGGNGEGSAVTAQWAPAVDVK
                     EEPDKFVLQADLPGVKPEEIDVNMEDGVLTIKGEKKTDAENDQEGYKRIERTYGSFYR
                     RFSLPDTANPDAISAKSNQGVLEIVIPKREAVQPKRINVTS"
     gene            complement(1694..2518)
                     /locus_tag="VFI43_00095"
     CDS             complement(1694..2518)
                     /locus_tag="VFI43_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7060556.1"
                     /translation="MEFLVEPLEYEFFRHGLLAALMVGSLCGLIGVYVVLRGMSYVGH
                     GLSHAAFGGAVVGFTLNLDFYAGAGAMGLLAALLINRITRNGQIKLDAAIGIVTTAMF
                     ALGVAIMSQVRTFSQNFEAALFGNILGITHEDLLIIGLVTACTIIAILFLYRPLLFST
                     FDNEAAQVFGIRTEAVQFMFSFLLTLSIIASMNVVGVTMIAATLITPALTARVMTASF
                     GRMLVYAFVIGAATGVGGMYLSYILNAASGATIVLFGALLFITAASIRRCREFLTH"
     gene            complement(2502..3302)
                     /locus_tag="VFI43_00100"
     CDS             complement(2502..3302)
                     /locus_tag="VFI43_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET7060557.1"
                     /translation="MNAAITLSEVAAGYGRNVVFSGLSLEIPVGRFAGIVGPTGCGKT
                     TLLKTILGTQRVFSGDVRLNGHPVANAPLGTIGYVPQLGSVDWNFPVTVEEVIMMGFY
                     TGKRIWPWASREERERTRELARRLGIYDCLHQPIMNTSGGQRQRAFLARALINNPQLL
                     VLDEPTSGVDIKTQHEVLHLLADINRDGITILLSTHDLNAVASHLPWVICFNKGLVAQ
                     GQPSDIFTNEVLTRTYGGDMVIVRHEGHFLVANNTLLHITGNHHGIPG"
     gene            complement(3320..4288)
                     /locus_tag="VFI43_00105"
     CDS             complement(3320..4288)
                     /locus_tag="VFI43_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET7060558.1"
                     /translation="MFRSILQGLKLLSFLLVLPLPAVSHGSDAIKKIRIVTTVAPITN
                     IVRNVAGGYGAVTGIVPDGTDSHTFEPVPTDARVLSTADIIILNGLGLEAPTLKLAQK
                     VKKSSTPILELGNGALRKEQWRYDFSFPRTGGHPNPHLWPDIALAMRYVEIVRDNLVA
                     LDPLNAPGYIENSNVYLAKLQELDSAIFSCIRTIPEKNRKLVTYHDSFAYFAPRYGVK
                     VIAAVQPSDFSEPGPREVIRIIKQIRQEKVPAIFGSEVFPSKVMEQIARETGVKFIDQ
                     LSDDELPPPPNNSFIGMMANNVATMTAALAGDARCIANVNTSNIQP"
     gene            4508..6847
                     /locus_tag="VFI43_00110"
     CDS             4508..6847
                     /locus_tag="VFI43_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7060559.1"
                     /translation="MPPATLWHDPGITTILAMSLLLIPSAFLIGNANAQQTLEVIDGK
                     PGTQLKKRKAVENAPSSQSTPLRVPEITDRANSGERQDFLTSQASNNPVMRLNEVTVT
                     GKERATSLLNEAQSASQGFIGQVQIRRRPLSRVGELVEFIPGMIATQHSGDGKANQYF
                     LRGFNLDHGTDFATFIDGVPINLPSHAHGQGYMDLNGLIPELIDNIQYGKGPYYADQG
                     DFSAAGHAKIGTFNSLPEGRGFAKFTAGSFGFYRGVAANSRRIGDGNLLYAGDVNTYN
                     GPWALAEQALKFNGMLKYSIDRNNWGGSVGIKAYQADWRATNQIPLFAVNEGLIGRFG
                     NIDPTDNGKTGRYTLHADWWQKENNYRRDASIYLVKYDIDLTSNFSGFINSVSSGTIL
                     PGGIIPSDQMNQTESRIYFGGKAGQSWFNKVAGFEMENSVGVQVRTDLNHVLLNHSNR
                     GVVYQNVSNNRIGQTSVGVYVQNETFWLPKLRTIAGLRGDIFNFDVHAITVPQNSGNK
                     TSSLANPKLSIIVGPWHRTEFYLNGGGGYHSNDARGVTATISTNRIVGNNFPPQLQAD
                     GLVRAWGAEAGLRTEAIEGLKSTLAVWYLHSGSELVFSGDTGTTEASGASTRYGIEWA
                     NFYKPVKWLTLDADFSFTHARYDRPQLNQASDPANRAYGFHIPNAVGRTISAGATLNL
                     PYNFFATLRLRHFGNVTVDTNNPVPPYSTSIVNFSTGYEIAKKLKIQLDVLNLLNAKS
                     FDIAYYYGYQTSPSAKARDGLVFHPIEPRMFRASLMYQF"
     gene            6871..7332
                     /gene="nikR"
                     /locus_tag="VFI43_00115"
     CDS             6871..7332
                     /gene="nikR"
                     /locus_tag="VFI43_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HET7060560.1"
                     /translation="MERLTISLDNQLSQQFDEFIRARGYTNRSEAMRDLIREHLEAAR
                     LENRGAGHCIATLSYIYNHHESDLAGRVTSVQHDHHDLTLSSMHVHLDHDNCLEIAIL
                     RGGIDEVEKFANLVIATRGVRNGKLHMIPVEIREEQHTSASPPHIHSNPLT"
     gene            complement(7412..8374)
                     /gene="yut"
                     /locus_tag="VFI43_00120"
     CDS             complement(7412..8374)
                     /gene="yut"
                     /locus_tag="VFI43_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HET7060561.1"
                     /translation="MMGWSLTVEAALPRPLRTVLRGVGQVFFCGNAVTGLIFLIALYI
                     GGLSAGLAATVGVISSTATAYVLGFSDDDIDAGLYGFNGTLVGPCLFLFLEHSPQLWL
                     YVVLASILSSVVLAALMRILAPYNVPASTSPFVLTCWMFLVAVYAFDNFARGPVLPPP
                     AIPSAVTGAGSVPTDLWFTALTKGVSEVMFADSVVVGILFLAGIAVVSFRGALMAACG
                     AAVGMVAAMLLGANLNAIEMGLYGFNPVLTMMAIGWVFLKPTGKSAALAFLAGILTVI
                     CQAGLANFLTPIGLPTLTFPFVLTMWMFLFAAGMSRYWSQPAAK"
     gene            complement(8445..9104)
                     /gene="ureG"
                     /locus_tag="VFI43_00125"
     CDS             complement(8445..9104)
                     /gene="ureG"
                     /locus_tag="VFI43_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET7060562.1"
                     /translation="MNKQSNPLRVGIGGPVGSGKTALCEALSKRMRDRYEMAVITNDI
                     YTKEDMEILLRAKALPPERLMGVETGGCPHTAIREDASINLEAIARMTADFPDLDLIL
                     VESGGDNLASTFSPELSDLTIYVIDVAAGEKIPRKGGPGITRSALLVINKIDLAPHVG
                     ASLEVMERDARKMRGERPFVFTNLHTGEGVDQVVDFIVKQGLLDEVKAKKAEESGQNV
                     H"
     gene            complement(9121..9801)
                     /locus_tag="VFI43_00130"
     CDS             complement(9121..9801)
                     /locus_tag="VFI43_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET7060563.1"
                     /translation="MMQSSSLLRLLQLASPSLPIGAYTYSQGLEASIEAGMVTNEDSA
                     RSWITESIGIASDFEAPIFWRLLKAFEARDGKTVTLWTERLVAARDTAEFRAETIQMG
                     YSLGKLVVDLGIADEALVSMLAGQADVPLPTALACAAQALGVPHESALLGMLFSLAEN
                     QVLVCVKSVPLGQLSGQRLLLSLHPVIQKAAEHARGLRDEQLSNWAPGLSLLSMRHET
                     QYSRIYRS"
     gene            complement(9807..10349)
                     /gene="ureE"
                     /locus_tag="VFI43_00135"
     CDS             complement(9807..10349)
                     /gene="ureE"
                     /locus_tag="VFI43_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HET7060564.1"
                     /translation="MLTLNTKIAHADDVFAQLILPYELRENSRLRTRLASGEEAAIFT
                     PRGTVLRNGDLLQGDDGRVIRIVAAEEPTYRITCSTPHDLLRCAFHLGNRHTQTQVGE
                     GFLRIYQDGVLKEMLEGVGATVVEESAQFEPEAGAYSGGGHHHAGDGHSHSHAHGHGH
                     GPLAPIPVHQKIHRPSDSKP"
     gene            complement(10352..12058)
                     /gene="ureC"
                     /locus_tag="VFI43_00140"
     CDS             complement(10352..12058)
                     /gene="ureC"
                     /locus_tag="VFI43_00140"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET7060565.1"
                     /translation="MSLKISRQAYAEMVGPTTGDRVRLADTELFIQIEKDFTTYGEEV
                     KFGGGKVIRDGMGQSQGNHADVMDTVITNAVIVDHWGIVKADVGLKDGKIAAIGKAGN
                     PDIQPNVTMMIGAATEIIAGEHMILTAGGIDSHIHFICPQQAEDAMMNGITTMLGGGT
                     GPAVGTAATTCTPGPWHIHSMLRASDGLVMNTGFFGKGNVSLPTPNEEQILAGACGLK
                     LHEDWGTTFAAIDNCLNVADKYDVQVAIHTDTINEGGYLENTIAAFKDRTIHTFHTEG
                     AGGGHAPDIIAVVGQENVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLNPAIPEDVAFA
                     ESRIRKETIAAEDILQDMGAISMMSSDSQAMGRIGEVVIRTWQTAHKMKIQRGTLQED
                     TSKNDNFRVKRYIAKYTINPAITHGVSEVIGSVEVGKYADLVLWRPAFFGVKPSMILK
                     GGMIAASLMGDPNASIPTPQPVHYRYMFGGYGGGIKTSSFTFTSQAAINSGLVDKLSL
                     DKNLIPIKNTRHLRKKDMIHNGATPKMEVDPETYEVRADGQLLTCGAEDVLPMAQRYF
                     LF"
     gene            complement(12055..12405)
                     /locus_tag="VFI43_00145"
     CDS             complement(12055..12405)
                     /locus_tag="VFI43_00145"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HET7060566.1"
                     /translation="MIPGEVFAEEGEIELNAGRKTRTITVSNGGDRPIQIGSHFHFYE
                     VNSAMKFDREAAYGMRLNIMAGTAVRFEPGQERTVELVELAGNRIVYGFNQKVMGKLK
                     DHRPTSQSDKGAEK"
     gene            complement(12447..12749)
                     /gene="ureA"
                     /locus_tag="VFI43_00150"
     CDS             complement(12447..12749)
                     /gene="ureA"
                     /locus_tag="VFI43_00150"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HET7060567.1"
                     /translation="MDLTPREKDKLIIFTAGLLAERRKARGLRLNYPEAVALITCAVL
                     EGARDGHTVAELMADGGKVLTRSDVMEGVPEMIPEIQVEATFPDGTKLVTVHNPIP"
     gene            complement(12889..13827)
                     /locus_tag="VFI43_00155"
     CDS             complement(12889..13827)
                     /locus_tag="VFI43_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET7060568.1"
                     /translation="MNDIPCFPNRRTMPESVVIHRLFPSEPVPVLSVGQPHPPLHARL
                     SLKFAEDAGATRLIERNHFGPLIIQKPFYPEGREVCQAVIVHPPGGVVGGDQMEIEAK
                     VGDRGRAQITTPGAAKWYKANGHVSRQNVTLEVGRGGSLEWFPQEAIFFDNAQVELEH
                     HVTLGKEACYIGCEILCFGRTASGESFSGGEIRQRISIRRDEKLMWYEQLRLSGGSPA
                     MANPLVLAGSTVCATLIAAGQSVSPDVIDAAREGAAGIVAQGDKAGISQLKGLVVARY
                     LGNSSEKARRVMLDVWKHLRPAMLGRTAVVPRSWNT"
     gene            complement(13888..15141)
                     /locus_tag="VFI43_00160"
     CDS             complement(13888..15141)
                     /locus_tag="VFI43_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060569.1"
                     /translation="MEQRKSYFGTLGLLIISAAALLSSLAYAEDVTLPSGPKYAIDDT
                     KWVTFGMGFRGTGRWAENKAADRFDEAFLIDNARLYVNGQVHRYIKFEINTECFFCNN
                     TQPGSNPKMSYNILDAIGKFEYNRYLNVWGGRMLVPTERGELSGPFFQATHDAFKTPF
                     FSQDFSTKFGVGGAGRYGRDDGGTYWGSLEPGFVKGTLQYAVGVYRGLTSAPGIGPNQ
                     GDSPVYAGRFTYNFLNPEKNPGYYTSSTYFGKAGDILALAFGASYQKDGAGSFAHRSN
                     FLGLVGDALLEKVLPRNLGVATVVAEYKQFYANYSPAAFTDPNCFCMFDGKSWTVTGL
                     YLTPMRIGIGKLQPYGRYTSIQPNHSSTREEIEGGVNYVIDGFKARISAYYQHGDLYT
                     KGLNYAPNVTGSKVDVFKLSFQLQM"
     gene            complement(15799..16434)
                     /locus_tag="VFI43_00165"
     CDS             complement(15799..16434)
                     /locus_tag="VFI43_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7060570.1"
                     /translation="MIIKVFLADDHRLFRDGLKRILAETADIKVVGEATDGMDAVEKM
                     CRGGWDVALLDVNMPGLNGLEVLKRIATSGAKLNVLMLSTYHEDEYAVRAIRAGASGY
                     LTKNSPTELLVSVIRRVAGGRKYVDPTLAEKLLFDLRPVTANPPHFVLSGRELSVLKL
                     IGVGTSLTEISRQLALSAKTVSTYRARILEKMNMQNNAQLVRYVIEHKLLE"
     gene            complement(16406..>17160)
                     /locus_tag="VFI43_00170"
     CDS             complement(16406..>17160)
                     /locus_tag="VFI43_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7060571.1"
                     /translation="EHKKAEARMLVEQELRVRSVAIREFAAHVERMREEDRKYIAREI
                     HDELGQLLAALHLEISLLKTSGKGKSNKIEKVEAIRCNMAELVDRANQSIRSVAEHLR
                     PASLGLGIAPALKRLTDEFRKYAGISCALQLMDEPVDLDEDQTVAIFRIVQESLTNVA
                     RHAEASRVEVSLSRGADYLVIEIRDDGKGFDPEGATKQKSFGLVGMRERAAVLGGNVE
                     VISGLQQGTAIRVRLPVKRNEEAHDHQGIPRG"
BASE COUNT         4011 a         4846 c         4393 g         3910 t
ORIGIN      
        1 aaaaaatggt agtgcgatcc aacctggata ggacggtcac ccgtgttacg cactttcagc
       61 tttagcgttc ttcgcccggc attgatctcc accggctgtt ggcagagaaa ccattcaccc
      121 gccttggtca ttgttggact cctttgataa atagacggtt aatcgacacc gggacggaga
      181 aacggattgc agccagaacg aaaggcatca gaaacaaaat ggcgtcagga aatcggatca
      241 ggcacggtga tgagctgcgt gccggtcagg aaagtggcct cgacctggat caggttgacc
      301 atttcaggaa cgccgtccat cacgtcgtcg cgtttaagaa cctgcttccc gatagccgtc
      361 acttcggaca aagtttttcc atcccgcgct ccttcgagaa gcgcttcgca gatgagcgca
      421 atggcctcgg cataattcag cttcaggccc cgatccttgc gtcgtcgggc gagatccgcg
      481 acctggtaga tcacgagctt ctccgtttcg cgaggggtga gcatcaagga ggagatgagt
      541 gtttcaggca tggcgaactc cccaaaaaat ccaggcacaa tcagtaacta gaagcgagtc
      601 gtacctcgcc gcctgcagct tcagatcatc cagcggcaat aatgcataac gcatgggcag
      661 atcgccacta aaggcaattt gcatacggtg ctcgtttatt tgtagaccct ttttccctat
      721 ttgcaagcca acgaacaatt tttttgcact caaaatacct acggcccagg ccattaaaat
      781 tcaatggctt gtccaccttt tgccgggacg atcgggcgag tcggagctac agaaaaatta
      841 aggaaaaatg ccgacggact gcggtttatc ggtgagtttc tgtgggagac ttttcatttc
      901 tctttaataa accgtggtct atctccagtt ttctctcctc atcagattgc ttcggcatga
      961 gacgaaaagc gggaagcgga gtgcgctcaa ctctcgatca aattcgtctg cgaccttgaa
     1021 aaacctgcga tccaccttat attgagtatt ggtaagggaa atacgcgctt caacactaat
     1081 ttcatctaaa ggaggcagta tggctattgc tcgttatgaa ccctggagtt tgttgcatca
     1141 gttacatcgg gaactggatc gggcccgcga gggaggaaat ggtgaaggat cggcggtcac
     1201 tgctcaatgg gctccagcag tggacgtcaa ggaggagccg gacaaattcg ttctccaagc
     1261 tgacctgccg ggcgtaaagc ctgaagagat cgatgtaaac atggaagatg gcgtgctgac
     1321 catcaaggga gaaaagaaaa cagacgctga aaacgaccag gaaggttata agcgcatcga
     1381 aaggacctat ggctcgtttt accgccgctt cagccttccg gataccgcga acccggatgc
     1441 gatctcagcc aaatcgaatc agggagtcct tgagatcgtc attccaaagc gcgaagcagt
     1501 ccaacctaaa cgcatcaacg tcacctcttg atgccgcatt gtccggtcag actttccaga
     1561 tagcctttca ggtatcagca cagtgagaaa aacgaagcgg agtatgccga aacttggcat
     1621 tctccgcttc gttatagcca acgcacgcag aatcctggct taacaccgat cctgccgatg
     1681 ttccttcacg ggactaatgg gtgagaaact ccctgcagcg cctgatcgac gcagccgtga
     1741 taaacagcag tgcgccaaac aatacgatgg tggcgcccga ggccgcattc aggatataac
     1801 ttagatacat gcctcctacc ccggttgccg ccccgatgac aaaagcgtaa accagcatcc
     1861 ggccgaaact ggcggtcatc acgcgggctg tcagcgcggg cgtgatgaga gtggcggcaa
     1921 tcatcgtcac cccaactaca ttcatggagg cgatgatgga gagcgtgaga aggaatgaga
     1981 acatgaattg cactgcttcc gtcctgatgc caaatacttg agccgcctca ttgtcgaacg
     2041 tagagaagag cagcgggcga tacaaaaata atatcgcgat aatcgtgcaa gcggttacca
     2101 ggccgataat cagcagatcc tcatgggtga tgcccaggat attgccgaat agagccgcct
     2161 cgaaattctg gctaaaggtg cgtacctggc tcatgattgc gacgcccagc gcaaacatgg
     2221 ctgttgtgac gataccaatg gccgcatcca gcttgatctg tccgttcctg gtaatccggt
     2281 tgatgaggag tgccgccagc agtcccattg cgcctgcccc tgcgtaaaaa tcgagattca
     2341 gggtgaaacc taccacggct ccgccaaatg ccgcatggga cagcccatgc ccgacgtagc
     2401 tcatgcctcg caataccacg tacacgccga ttaatccgca cagcgagccg accatcaagg
     2461 cggccaggag gccgtgccgg aaaaactcgt attcgagggg ctcaaccagg aattccatga
     2521 tgattacccg taatatgcag gagcgtgttg ttggcgacca gaaaatggcc ctcgtgcctg
     2581 acgatgacca tgtcgccccc ataagttctt gtgagaactt cgttcgtaaa aatatcactg
     2641 ggttgaccct gcgccactag gcctttattg aagcagatca cccagggaag atgtgaagca
     2701 accgcattaa ggtcatgcgt gctgagcagg atcgtgattc catccctgtt aatgtccgcc
     2761 agcagatgca acacttcatg ctgggtcttg atatccaccc ccgacgtcgg ctcgtcgagg
     2821 acgaggagct gtggattatt gattaacgca cgcgcgagaa aggcgcgttg acgctgtccg
     2881 ccggaagtat tcatgatggg ctgatggaga caatcgtaaa tacccagcct ccgggccagt
     2941 tctcgcgttc gctcgcgctc ttccctgctt gcccacggcc agatacgctt acccgtatag
     3001 aaccccatca tgatgacttc ttctaccgta accgggaagt tccagtcgac acttcccaac
     3061 tgtggaacgt acccgatggt gcccagcggg gcgttcgcca cgggatggcc attcaagcgt
     3121 acgtcgcctg aaaaaacccg ttgcgtgcct agaatggtct taagcaaagt cgtttttcca
     3181 cagcccgtag gccccacaat gccggcaaac cggcctaccg gtatttccag cgaaaggccg
     3241 gaaaatacga cgttacgacc gtaaccggca gccacctcgc tcaatgtgat cgccgcgttc
     3301 atatatagat catcgtaatt cagggctgaa tattgcttgt gttcacattt gcaatacaac
     3361 gtgcatcgcc ggcgagcgca gccgtcatgg ttgcgacatt atttgccatc attccgataa
     3421 aggaattatt cggcggtgga ggcaattcgt catccgatag ctgatcgatg aacttcactc
     3481 cggtttcgcg ggcaatttgc tccatcactt tgctcggaaa cacctcggaa ccaaaaatgg
     3541 cagggacctt ctcctgcctg atctgcttga taatacggat aacttccctt gggcccggct
     3601 cggaaaaatc ggatggttga acagctgcga tgacctttac cccatagcga ggtgcgaaat
     3661 aagcaaagga atcatggtaa gtgaccagct tgcggttctt ttcgggaata gtcctgatgc
     3721 aactgaagat cgcgctgtca agttcctgca atttcgcaag atagacgtta ctgttttcga
     3781 tatagccggg tgcgtttaga gggtcgagcg caaccagatt gtccctcacg atctctacat
     3841 aacgcatcgc aagcgcgatg tcaggccaca gatgtggatt cgggtgccct cccgtccggg
     3901 gaaagctgaa atcgtatcgc cattgttcct tgcgaagcgc gccgttgcct agctcaagaa
     3961 tcggagttga actcttcttg actttttggg cgagcttcaa agtcggcgcc tcaagcccga
     4021 gtccattcag gatgatgata tctgcagtgc taagcacccg cgcatccgtg ggcactggct
     4081 caaacgtatg agaatcggtt ccgtcgggca cgatgcccgt cacagcgccg tagccgccgg
     4141 caacgttccg gacaatattg gtgatgggcg caaccgtggt aacgatgcgt atttttttta
     4201 ttgcatccga accatggctc acggcgggta aaggcaaaac cagcaggaat gataaaagct
     4261 tcaagccctg aaggatcgat cgaaacatgt tagctaccgg cataggggga tttgcgggca
     4321 ttcgatgtgt ctagctagtt tatagcttcg aaaatgatat gaacagttat aaatatttca
     4381 tactgaaaaa ataggaggcc tccaacgctt gacgcacgga tacagcagtg attgaatgta
     4441 tgaatattac aagaatcttc atacattatc ccggttcacg ccgtcgagag atacatgatt
     4501 taaatccatg ccccccgcga cgctttggca cgatccaggg ataaccacta tcctggccat
     4561 gtccctactt ctgatccctt cggcttttct tattggcaat gccaacgcac agcaaaccct
     4621 tgaagttatc gacggcaaac ccggaacgca attgaaaaag cgaaaagccg tagaaaatgc
     4681 tccctcatcc cagagcaccc ccctgcgtgt gcccgagatt accgacaggg caaattcagg
     4741 cgaaaggcaa gatttcctta caagccaggc gagcaataat cccgttatga gactgaacga
     4801 ggtcactgtg acgggaaaag agcgcgcgac aagtcttctc aatgaagcgc aatccgcatc
     4861 ccagggattc atagggcagg tgcagatcag gaggcgcccg ctttcgcgcg tcggcgagct
     4921 tgtcgagttt attcccggga tgatcgccac ccagcacagc ggtgacggaa aagccaatca
     4981 atatttcctg agggggttca atctcgatca cggcaccgat tttgcgacct ttatcgatgg
     5041 cgtgcctatc aatctcccca gccatgccca tgggcagggt tacatggatc tcaacggact
     5101 tattcctgag ctgatcgaca atatccagta tggaaaaggg ccgtattacg cggatcaggg
     5161 agatttttca gcggcagggc atgccaagat cgggaccttc aattcgcttc cggagggacg
     5221 gggttttgcg aaattcactg cgggatcatt cggcttttat cgcggagtag cggccaactc
     5281 gagaagaatc ggggatggca acttgctcta tgcgggcgac gtaaatactt ataacggtcc
     5341 ttgggcgttg gcggaacaag cgctcaagtt caacgggatg ctcaaataca gcatcgatag
     5401 aaacaattgg ggtggaagcg tgggcatcaa ggcgtaccag gcagattggc gcgcaaccaa
     5461 tcagataccc ctattcgcag tgaatgaagg gctgattggg cgatttggca atattgatcc
     5521 caccgataac gggaaaaccg gccgctacac cttgcacgct gattggtggc aaaaggaaaa
     5581 taactatcgg cgggacgcct ccatttacct ggtcaaatat gacatcgacc tgacttcaaa
     5641 tttcagcggc ttcatcaatt cggtttccag tggcacgatt cttccaggcg gaatcattcc
     5701 gagcgaccag atgaatcaga cggaatcccg aatctatttc ggcggcaaag ccggacagag
     5761 ctggttcaac aaagtagcgg gcttcgagat ggaaaactcc gtaggggtcc aggtacgcac
     5821 ggatttgaat cacgtgctgc tgaatcattc caaccgcgga gtcgtgtatc agaacgtgag
     5881 taacaaccgg attgggcaga ccagtgtcgg cgtttacgtg caaaacgaga cgttctggct
     5941 gcccaagctt cggactattg cgggattgcg aggtgacatc ttcaattttg acgttcacgc
     6001 cataacagtg ccacaaaact ccggcaacaa gacctcttcc ctggcaaacc ccaaattgag
     6061 cattatcgtg ggcccctggc atcgcaccga attttatcta aatggtggag gaggttatca
     6121 ttccaatgat gcgcgcggcg taacggcgac catcagcaca aatcggattg taggcaacaa
     6181 ttttcctcct cagttgcagg ctgatgggtt agtgcgcgcg tggggcgcgg aggcgggctt
     6241 gcgcacggag gccattgagg gcctcaaatc caccctggcg gtttggtacc tgcattccgg
     6301 ctcggaactg gtattcagcg gcgatacagg gaccaccgaa gccagcggtg caagcacccg
     6361 gtacggcatc gagtgggcaa atttctacaa acccgtaaaa tggttgacgc tcgatgctga
     6421 cttttccttt acgcacgcgc gctatgatcg acctcagctc aaccaggcga gcgatccagc
     6481 gaaccgggct tacgggtttc atattcccaa tgcagtcggc agaaccatca gcgcgggcgc
     6541 gaccttgaat ttaccgtata atttctttgc cacccttcgc ctgcgccact ttggcaatgt
     6601 aacggtggac accaataatc ccgttccacc ttatagcacc agcatcgtga atttctcgac
     6661 agggtacgag atcgcgaaaa aacttaaaat ccagcttgat gtactgaatc tgctgaacgc
     6721 taaaagcttc gacattgcct attattatgg gtatcagacg agcccttcgg ctaaagcaag
     6781 agatggcctt gtttttcatc ctatcgaacc gcgcatgttt cgcgcgagcc tgatgtatca
     6841 attctaagac cggccttcgc tggaaagaca atggaacgcc ttaccatatc gctggataac
     6901 cagctttccc aacagttcga tgagttcata cgcgcgcggg gttataccaa ccgatcagaa
     6961 gccatgcgcg acctgatacg ggagcatctc gaagctgcac ggctcgaaaa caggggtgca
     7021 ggacactgca tagctaccct cagctatatc tacaaccatc atgagagcga tctcgctggg
     7081 cgcgtgacct ccgtacagca tgatcaccat gatttgaccc tctccagcat gcacgtccat
     7141 ctggatcatg acaactgcct ggaaatcgcc atcctgaggg gaggaattga cgaggttgaa
     7201 aaatttgcca acctcgtcat tgccacgcgc ggagtgcgaa acgggaaatt acacatgatt
     7261 cccgtggaga tcagagagga acagcatacg tccgcctcgc cgccacacat ccacagcaac
     7321 cctcttacat gatccacaaa gatagaagct gccgctttta cgcctggcgc tcgtccctac
     7381 agatacactg aaagcagaaa acagtcggct tctatttggc tgcgggctgc gaccaatacc
     7441 tcgacatccc tgccgcaaat agaaacatcc acatcgtcag cacaaaggga aacgtaagag
     7501 taggcagccc gatcggggtg aggaagttcg caagtccagc ctggcatatg acggtcagga
     7561 ttcccgccag gaacgccaag gcggcgctct tccccgtagg cttgaggaag acccagccaa
     7621 tcgccatcat ggtcagtacg gggttgaagc cgtacaagcc catttcgatc gcattcaggt
     7681 ttgctcccag aagcatggcc gcaaccatgc cgactgctgc gccgcaggca gccatgagcg
     7741 cgcctcggaa ggaaacgacc gcaataccgg ccaggaacag aatccccaca accacgctgt
     7801 cagcaaacat cacctcgctt accccctttg taagcgcagt gaaccagaga tctgtcggca
     7861 ccgatccagc tcctgtcacg gccgaaggaa tggcaggtgg aggcaaaacc ggcccccggg
     7921 caaagttgtc gaacgcgtat actgcaacca ggaacatcca gcaagtcaat acaaacgggg
     7981 acgtcgaagc gggaacgtta tagggagcga gaattcgcat caatgccgcc agcacgacgc
     8041 tcgataatat cgacgcaagc acgacataga gccagagttg cggagaatgc tccaggaaca
     8101 aaaacaggca tggccccacc agcgttccgt tgaagccgta taaccccgca tcgatatcat
     8161 catcggaaaa ccccagcacg taagcggtgg ctgtactgct tatgacgcct accgtcgcag
     8221 caagcccggc gcttaagcct cctatataga gggcaatcaa aaatatgagg ccggttacgg
     8281 cgttgccgca aaaaaacacc tgccccacac cccttagaac cgttcgtagg ggacgaggaa
     8341 gcgcggcttc gactgttaac gaccaaccca tcatgatcgg ctccggattt cgctattgga
     8401 catcacttgg ctatacattc gaacaactga ccgctgtttt tcccttaatg cacgttctgc
     8461 ccggactcct ccgccttttt cgccttgact tcatccagca acccctgttt gacgatgaaa
     8521 tccacgacct gatcgacacc ctcgccggta tgcagattgg taaatacgaa cgggcgttct
     8581 cccctcatct ttcttgcgtc ccgctccatc acttccaggc ttgcgcccac atgaggcgca
     8641 aggtcgatct tgttgatgac cagcagtgct gaacgggtaa tccccgggcc gccctttctc
     8701 ggtatcttct cgccggcggc gacatcgatc acgtaaatgg tcaggtcgga aagctccggg
     8761 ctgaaggtgg atgccaggtt gtcccctccc gactcgacca ggataagatc gagatcaggg
     8821 aaatcggcgg tcatccgtgc aatcgcctcg aggttgatcg aagcatcttc cctgatcgcg
     8881 gtgtgcggac aaccgccggt ttccactccc atcaggcgtt ccggaggcaa ggccttggca
     8941 cgcagcagga tttccatatc ctccttggta tagatatcgt tggtgatcac ggccatctca
     9001 tagcggtcgc gcatcctttt gctcagcgcc tcgcacagtg cggtttttcc ggaaccgacc
     9061 ggcccgccaa tgccgactct caatggattc gattgtttat tcatggtctc aatgtagtat
     9121 tcaagatcga taaatgcggc tgtattgcgt ttcatgccgc atcgacagca atgacagccc
     9181 cggcgcccag ttggacaact gctcgtcgcg caacccgcgc gcatgttccg ccgctttctg
     9241 gataacagga tgcagggaaa gcagaagacg ctgaccggat agctgcccga ggggaacgga
     9301 cttcacgcac accagcacct ggttctcggc caatgaaaac agcattccca gcagtgcgct
     9361 ttcgtgtggc acgcccaggg cctgcgccgc acaggcaagc gcagtcggca ggggaacgtc
     9421 agcttggcct gcaagcatgc tcaccaatgc ctcgtctgca atcccgagat ccaccacgag
     9481 cttgccgagc gaataaccca tctgtatggt ttctgcccga aactcggccg tgtcgcgggc
     9541 ggcaacgagg cgttctgtcc acaacgtcac cgtcttcccg tcgcgcgcct cgaaagcttt
     9601 cagaagacgc cagaagatgg gggcctcaaa atccgaggca atcccaatcg actcggttat
     9661 ccagcttcgc gcggaatctt cattcgtgac catgcctgct tcgatggatg cttccaggcc
     9721 ctgcgaatac gtataagcgc cgatgggcag cgaggggctt gcgagttgca acaagcgaag
     9781 cagcgagctc gattgcatca tgctcgtcaa ggcttggagt ccgaaggccg gtgaattttc
     9841 tggtgcaccg gtattggcgc cagcggacca tggccgtgac catgagcatg agaatggctg
     9901 tggccatctc cggcatggtg atgccctccg ccactataag cccctgcttc cggttcaaat
     9961 tgcgcacttt cttccaccac agttgctccc acgccttcca gcatctcctt caatacccca
    10021 tcctggtaaa tgcgcaggaa cccctcgcct acctgggtct gggtatgacg gttgccaagg
    10081 tggaacgcgc agcgcagcaa atcgtgaggc gtactgcagg taatccggta ggtaggctct
    10141 tcggctgcga caatacggat cactcttcca tcgtctccct gaagaagatc cccattgcgc
    10201 agtacagtac ccctgggagt aaaaatcgca gcttcttctc ccgaagccag ccgggtgcgc
    10261 aagcggctgt tttcgcgcaa ctcataaggt agaatgagct gcgcaaaaac gtcatccgca
    10321 tgcgctattt tcgtattcaa ggtaagcatg cttaaaacag aaaatatcgc tgggccatgg
    10381 gcaggacatc ctctgcaccg catgtgagga gttgtccgtc cgcccggacc tcgtaggttt
    10441 ccggatcgac ttccattttg ggcgtagcgc cgttgtggat catgtccttc ttgcgcaaat
    10501 ggcgagtgtt tttaatcgga atcaggtttt tgtcaagact gagcttgtcg accagccccg
    10561 agttgatcgc tgcctgggag gtgaacgtga agctcgaggt cttgataccg ccaccatatc
    10621 caccgaacat atatcggtag tgcacaggtt gcggagtcgg gatcgaggcg ttcggatcgc
    10681 ccatcagcga ggcagcgatc attccgccct tcaggatcat tgacggcttg acgccgaaga
    10741 aagcgggcct ccacagcacc aggtcagcgt acttccccac ttcgaccgaa ccgattacct
    10801 ccgaaacgcc atgcgtgatg gcaggattga tggtgtattt ggcgatataa cgtttaacgc
    10861 ggaaattgtc attcttggag gtatcttcct gcaaggtgcc gcgctggatt ttcatcttgt
    10921 gcgcggtctg ccaagtacgt atcactacct ccccgatacg ccccatcgcc tgcgaatccg
    10981 atgacatcat cgagatggcg cccatatcct gaaggatatc ctcggccgcg atggtttcct
    11041 tgcgtatgcg cgattccgcg aatgcaacat cctccgggat ggccggattc agatggtggc
    11101 ataccatcaa catatccaga tgctcatcca gcgtgttgac ggtatagggc cgggtcgggt
    11161 tggtggatga gggcagaacg ttttcctgac ctacgacggc gataatgtca ggcgcatgcc
    11221 ctccgcccgc gccttctgtg tgaaaggtat ggatggtgcg gtctttgaaa gcagcgatgg
    11281 tattttccag gtaaccgcct tcattgatgg tatcagtgtg aatggcgacc tgtacgtcgt
    11341 atttatcggc aacgttcagg caattgtcga tagccgcgaa ggtcgttccc cagtcttcat
    11401 gcaacttgag gccacatgct ccggcaagga tctgctcttc attgggagtt ggcaggctca
    11461 cattgccttt accaaaaaaa ccggtattca tcaccaggcc gtctgatgct ctgagcatag
    11521 aatggatatg ccaggggcca ggcgtgcagg ttgtcgccgc agtacccaca gccgggccgg
    11581 ttcccccacc cagcatggtt gtgattccat tcatcatcgc atcctcggct tgttgaggac
    11641 aaatgaagtg aatgtggctg tcgatccccc ccgcggtaag aatcatatgc tccccagcaa
    11701 tgatttcggt cgccgcgcca atcatcatcg tcacattagg ctggatatcc gggtttcccg
    11761 ccttcccgat cgctgcaatc ttgccatcct tgagaccgac gtcagccttg acgatacccc
    11821 aatggtccac gatcaccgca ttggtaataa cagtatccat gacgtccgcg tgatttccct
    11881 gggattggcc catgccgtcc cgaatcactt ttccgccccc gaacttgact tcttccccgt
    11941 aggtcgtgaa atctttttcg atctggatga acaactctgt atcagcgagg cggacccgat
    12001 ccccggtcgt gggtccgacc atctccgcat aagcttgcct ggaaattttt agactcattt
    12061 ttctgctcct ttatcggatt gggatgtcgg ccggtggtcc ttcagtttgc ccatcacttt
    12121 ctgattaaaa ccgtacacga tccggtttcc cgcaagctcg acgagttcaa cagtacgttc
    12181 ctgccccggc tcaaagcgaa ccgctgttcc cgccataata ttcaggcgca tgccataggc
    12241 cgcctcccgg tcgaatttca ttgccgaatt gacttcgtaa aaatgaaaat gggatccgat
    12301 ctggatgggg cggtcgcccc cgttggaaac agtgatcgtc cttgttttcc ttcctgcatt
    12361 caactctatc tcaccctcct ccgcgaatac ttctcccgga atcatcggta ctctggccat
    12421 cataatctcc tgtatatgtc gcgatctcaa ggaatgggat tgtgcaccgt gacaagtttg
    12481 gttccatccg ggaacgttgc ttccacctgg atctcaggaa tcatctccgg aacgccttcc
    12541 atgacgtcgg aacgcgtcag cacttttccg ccgtccgcca tcaattccgc aacggtatga
    12601 ccgtcacggg cgccttcgag caccgcgcag gtgatcaggg caaccgcttc cgggtaattg
    12661 agtcgcaggc cgcgggcctt gcgcctttcg gccagcaatc cggcggtaaa aatgatcaac
    12721 ttgtcttttt ctcttggtgt cagatccata tgcttcctcc tcgataaaca gatgattgtt
    12781 caatttctgt tccaaaagga tcagaaaccc gggtcgaaaa tcgaccggag cgttgcctcg
    12841 aaacgcgttt catcttgaat atagctctgc ggcagttgcc acgccacctc atgtattcca
    12901 gctgcgaggt actaccgccg tacggccgag cattgcaggc cgcaggtgct tccagacatc
    12961 gagcatgaca cgtcgcgcct tttcactcga gttgcccagg tagcgcgcga ccaccaatcc
    13021 tttcaactgg gagatccccg ctttatcccc ttgcgccaca attccagcag cgccttcccg
    13081 cgccgcgtct atgacgtccg gtgaaacact ttgtcctgcc gcaatcaaag ttgcacaaac
    13141 cgtgctcccc gcgagcacaa gcgggtttgc cattgcaggg ctgccgccgg acagtctcaa
    13201 ttgttcatac cacatcagtt tttcatcccg gcgaatgctg atccgctggc gaatctctcc
    13261 cccgctgaat gattccccgg aagcggtgcg cccgaagcat aaaatctcgc aaccgatata
    13321 gcaggcctcc ttccccagtg tcacatgatg ctcaagctcg acctgtgcat tgtcgaagaa
    13381 aatcgcctcc tggggaaacc actcgagcga acctcctcga cccacttcca gcgtcacgtt
    13441 ctgtcgtgac acgtgaccgt tggccttgta ccattttgcc gcccctggag tagtgatctg
    13501 tgcccttccc cgatcgccga cctttgcttc gatttccatc tgatcgccac cgaccactcc
    13561 cccaggagga tggacgatga ccgcctggca tacctcccgc ccttcgggat aaaaaggctt
    13621 ttgtatgatc agtggtccga aatggttgcg ttcgatcagg cgggtggctc ctgcatcctc
    13681 cgcgaatttc aaagacaggc gcgcatggag tggcgggtgc ggctggccta cagacaaaac
    13741 gggcacaggt tccgagggga aaagtcgatg gattacaacg ctctccggca ttgtccgcct
    13801 attggggaag cagggaatgt cgttcatttt ttgttctttg tcggttgctg ctcttatcgc
    13861 agcccctacc tgcagtcgat acaggaatta catctgcaac tggaaggaca acttgaaaac
    13921 gtccaccttg ctcccggtta cattgggtgc ataattcagg cccttcgtgt aaaggtcgcc
    13981 gtgctgatag taagccgaaa tgcgcgcttt aaagccatcg atcacatagt tcaccccacc
    14041 ctcgatttct tcgcgcgtac tgctgtgatt cggctgaatg ctggtgtatc tcccgtacgg
    14101 ctggagtttt ccgataccaa tccgcatggg ggtcaggtac aggcccgtga ccgtccagga
    14161 ttttccatcg aacatgcaaa aacagttggg gtccgtaaac gctgcaggtg aataatttgc
    14221 ataaaactgc ttgtactccg caactacggt cgccaccccc agatttcttg gcagcacctt
    14281 ttccaggagc gcatccccaa caagtccgag gaagttgctt cgatgggcga acgaaccggc
    14341 gccatccttc tggtacgacg cgccgaatgc cagcgcgagg atatcgcccg ccttgccgaa
    14401 gtacgtgctg ctcgtgtagt agccggggtt tttctcggga ttcaggaaat tgtaggtgaa
    14461 gcgcccagcg tataccgggc tgtcaccctg gttcggtccg atgccagggg cggaggtcaa
    14521 tcctcggtat acacccaccg catattgcaa cgtacccttc acaaaaccgg gttcaaggct
    14581 tccccagtag gtcccgccat cgtcacggcc atatcggccg gccccgccca cgccgaattt
    14641 agtgctgaaa tcctgcgaga agaagggtgt tttgaaggca tcgtgggtag cctggaaaaa
    14701 aggcccgctc aactcacccc gttcagtagg taccagcatg cggccacccc agacgttgag
    14761 atacctgttg tactcgaact tcccgatcgc atcaagaatg ttgtaagaca tcttgggatt
    14821 actgcccggc tgggtgttat tacagaaaaa gcattcggta ttgatttcaa acttgatata
    14881 ccggtgcact tgtccattga cgtacaaacg agcgttgtcg ataagaaacg cttcgtcgaa
    14941 gcggtctgca gccttgttct ctgcccagcg gcccgtgccc cgaaaaccca tcccgaatgt
    15001 tacccatttg gtgtcatcga ttgcatactt cggaccgctg ggaagcgtga catcctctgc
    15061 gtatgccagg gatgataaaa gcgcggccgc cgaaatgatg agcaggccga gcgtgccaaa
    15121 ataacttttt cgttgctcca ctaccccgcc ttttaatggc cgattttgga taaccctttt
    15181 cccacaggcg gcttcagccg ggaacagcgt gtatgcgcac atcttaaatg tcctttttat
    15241 cctttcctga gctttccctc ggggaagctc ttcctcgtta ctcatggtgg atgggataat
    15301 gatgaaggaa tttgtagtgc gcgtcatcag aaaatatcca caacctgcaa attttcgatg
    15361 tgaacacttc tgtaataatt aatgcaggga caagcatcgg cagtcaaacg aatgaaggta
    15421 tcggtagaaa tgaaggcatt ggtagaggta cccaagtcgc ctgttatccc gtttcgctcg
    15481 tttttcactt cgttatccct tgaaaaaaag acgcgactgt tgcggcagca gcctggaacg
    15541 caactcctga tgcctcgctc atgccctcac tcattgcccc gcttgcgcca cgttttctgc
    15601 gccaactcgg ggattatgaa atgatcttca agagcctatc attaatcgta cggagtgaat
    15661 gtagggaatt ccttacacct tgttggcggg agaaatcaaa gaagaaggtt gatatctgga
    15721 aatggcaata acgcaaaact cagctggaaa gcattcattc cctcatgaag cgtggcttcg
    15781 acgggaataa ttcgcgggtt attcaagcaa tttatgttcg atgacgtagc ggaccaattg
    15841 cgcgttgttc tgcatattca ttttttccag tattcgcgcc cggtaggtgc tcacagtttt
    15901 agcgctcagg gcaagctgcc gcgaaatttc ggtcagcgat gtcccgactc cgataagctt
    15961 gagcacgctc agctcccggc cggaaagtac gaaatgcgga gggttcgccg ttacagggcg
    16021 gagatcgaac agcaattttt ctgccagcgt tggatcgaca tatttacggc cgccggccac
    16081 acgccggatg accgatacca gcagttccgt cgggctattc ttcgtcaaat agccggaggc
    16141 gccggcacgt attgcccgga cggcatattc atcctcatga tatgtgctga gcatcagcac
    16201 gttaagtttt gcaccggaag tcgcgattct ttttaaaact tccagcccgt tcaagcctgg
    16261 catgttgaca tcaagcaacg cgacgtccca tccaccccgg cacatcttct ccaccgcatc
    16321 cattccatcc gtggcttcgc caaccacttt gatgtctgcc gtttccgcca ggatccgctt
    16381 cagcccgtcg cggaacagcc tatggtcatc cgcgaggaat accttgatga tcatgagctt
    16441 cctcgtttcg ctttaccggt aggcgcaccc tgatagccgt tccttgctga agtccgctga
    16501 tgacttcgac atttccgccc agtacggctg cccgctcgcg catccctacc aacccgaatg
    16561 atttttgctt cgttgcgcct tctgggtcga atcccttgcc gtcgtcccgt atttcgataa
    16621 caagataatc agcacctcgg gagaggctga cttcaacacg acttgcctct gcgtgccttg
    16681 ccacattcgt caatgattcc tgaacaatcc ggaatattgc cactgtctga tcttcgtcca
    16741 gatcgaccgg ttcatccatc aactgcagcg cacaggatat gccagcgtat ttcctgaatt
    16801 catcggtcag ccttttaagc gctggagcga ttcccaaccc gagcgaagcg gggcgtaaat
    16861 gttcggcgac gctgcgaatg ctctggttcg ctctatccac cagctccgcc atattgcacc
    16921 ggatagcttc aactttttca attttattgg acttcccttt gccagaggtt ttcaatagcg
    16981 agatttcaag atgcaaggca gccagcaact ggcccagttc gtcgtggatc tcacgggcga
    17041 tatacttgcg atcctcttcc cgcatccgtt cgacatgcgc tgcaaactcg cgtatggcta
    17101 cgctacgcac acgcaactcc tgctccacga gcatgcgcgc ctccgctttc ttgtgctcca
//
LOCUS       DASSHJ010000003         9447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_757654, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000003 DASSHJ010000000
VERSION     DASSHJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757654"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..682
                     /locus_tag="VFI43_00175"
     CDS             350..682
                     /locus_tag="VFI43_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7060572.1"
                     /translation="MEGIFLKFYVSEYREHKGILLFEWLLERAKKCGFHGGTALRAVA
                     GFGRHGVLREETFIELAADLPVEVSFVLGTEEAKHFLDLLEAENIDLFYVKFPVEYGF
                     LPTDQVVD"
     gene            868..2139
                     /locus_tag="VFI43_00180"
     CDS             868..2139
                     /locus_tag="VFI43_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7060573.1"
                     /translation="MALAQAEAIPAMDGQVAITEQQAIALFYQRNLSLIAASLNIDEA
                     RAREIIAAAIPNPVLSVTVAELNRKIFRAENHDQPLPAVIPQVQQLIETAGKRRLRIE
                     SSELATEAVGYDLRDVARTLTNAVRRSYYSLILAQKALKVANDNFEHYREILRVNEIR
                     LKVGDVAAMDFVRIEVEGLKAQSDQDQALAAVKQARADLLLLLGWPENSVDIVAVEAW
                     PEISPYIALSRQDDLVLRALERRPDMQAARIRIAQARKMVTLAQRQVIPDVTISAFYD
                     RDPGNFFARSGGVGISVPIPLFYQQKGEISKARVTLTSAELAVAHAEQAIRAEVLKAA
                     SAWQSADSIARRFETSVVGRIETLRKSQELAYQKGAIGVLELIDAQRTYRVVMLDYYA
                     ALANRSKAWADLLMGYGEDPAQFLHNPLPGR"
     gene            2518..3477
                     /locus_tag="VFI43_00185"
     CDS             2518..3477
                     /locus_tag="VFI43_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7060574.1"
                     /translation="MEPVAAKIVYDESHTVRVFSPIAGRVTGAIAPTGTALRAGEVLV
                     ELDSPELGQAQAIYSDALADLNLAERAYQRTKELVDNGIVPRKELDQAEDALSHAHSE
                     SERARLKLANLGVRSRRTDNRFVLHAPISGTVTERNINPGMEVRADLTAPLFVISRLD
                     QLWVQMDIFEKDIGLIHVGEKVLLHVPAYPDKTFTANVSYISQIVDENTRAIKVRCIL
                     PNPDFRLLPAMYASAEVQSDPGDLAIVVPLTALFTEDESEWVYINIGNNHYQKRHVKV
                     GLRPKDRAVILEGVKPGERVVVKGALLLRIEQDNEAQSGEHAQ"
     gene            3474..6554
                     /locus_tag="VFI43_00190"
     CDS             3474..6554
                     /locus_tag="VFI43_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET7060575.1"
                     /translation="MINRIIAFCLQQRLLIISTAILLGAIGILTFERLPIKAFPDVQN
                     VFVQVVTQYPGQAPEEVEKLISLAIEKEMNGLPHMITMRSVSIFGLSVVTLIFDDEAE
                     DYFSRQLVLERLKGVSLPAGVKPLLGPLTTGIGEIYRYRLEAPGVPLVEQRAIQDWLI
                     ERTLRSVQGVADVVAFGGGIKQYQVQVDSNKLRNYKLTLPDVYEAIAGNNANTGGGYI
                     DHGNEAMVVRGTGLLKSTEEIGRVAITTQEGVPVFVKTVADIVIGPQPRVGIVGFDQF
                     DDVVQGIVLLIKGSNATVVLTGVKEKIDYLNRYGLPQGVRIIPIYDRTELIQHTVNTV
                     ERNMAEGAVLIFAVLVVFLRRFWASLVVIVVVPLSLLFAFILIDMKHVSANLISLGAI
                     DFGIIVDSAVVLVEAIMVKVTLDMNQGASLARLRQSMLSTTAEMARPILFSKAILIIA
                     FIPIFTFQRVEEKIFSPMAFTLTFALLGSLIISLTLVPVLLSYLLGPRLTESRNPLIH
                     WMEKRYLKILQAVLHHPRRLFLGASVALALSIASWPIIGTEFMPKLDEGNVWLTITLP
                     TPVSLEKAKELERHVRASLLEFPEARYVLTQLGRPEDGTDPKGFNNLEVLIDLTPRKT
                     WRYARKDELVGAMARRLAVFPGLQFNFSQVIQDNVEEAISGVKGEIAIKIFGDDLKIL
                     QQKANQITGILRSIEGATDVAAEHQSGLSQLVISIDREKIARFGINIADVEDVIEMAV
                     GGKAATQMLEGERRFDVAVRLKADARDSVGAIEDITVLSPGGGRIPLAQLAEIKTSQG
                     ASRISRENNMRLIAIKCNLIGRDQGSFVAEAQKKISRQVHLQPGYHMVWSGQFENQQR
                     AMKRLSFIVPVSFALIFVLLFWAFRSVRNASLIVMNVPFSLIGGLVALLLTGIHLSVS
                     AAVGFIALFGIAVQNGVILLSQINALRQEGVPINEAIIQGSVSRLRPVVMTALMAMLG
                     LLPAAISTSVGSETVKPFAVVIIGGLVSATILTLTLLPALYRNFEEWNRTKVLP"
     gene            complement(6923..7231)
                     /locus_tag="VFI43_00195"
     CDS             complement(6923..7231)
                     /locus_tag="VFI43_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060576.1"
                     /translation="MSVVLPAAVVFATSAFAGEKERIQWMDVPPAVQKTILNHAGGGK
                     IEEIEKETKIQKVRVLHFDSGKTVTVYEAEVEKPDGKEIEIDVGEDGKLIKIKDEKGV
                     "
     gene            7481..7804
                     /locus_tag="VFI43_00200"
     CDS             7481..7804
                     /locus_tag="VFI43_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7060577.1"
                     /translation="MKEIRAYIEPFMLSRVTQALLEITDFPGMSVSDCEGFGGEKVID
                     MDFTPFLHRKRVEIFAPDELVEQIFNTLMLAANTHQRGAGKIYVLDVEKSGHISRGTQ
                     KDERS"
     gene            complement(7969..8262)
                     /locus_tag="VFI43_00205"
     CDS             complement(7969..8262)
                     /locus_tag="VFI43_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060578.1"
                     /translation="MKRSILLSAAMVIATSAYAEREEKVRWSQLPSAVQKTITDNAGG
                     GKVEEIDKETRTKDGKTVTTYGAEVNGPGGKKVEIKVGEDGRLIELKDDRNVS"
     gene            8385..8813
                     /locus_tag="VFI43_00210"
     CDS             8385..8813
                     /locus_tag="VFI43_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7060579.1"
                     /translation="MDKYEVEAFSPFRALLKDYLLWILAGLLAVLYLMKPLRLSSYLR
                     LVDWPTIAGVAGLLVLAKGIEFSGYLHRLGKRVIEAMPTGRSLATLLVLAAALLSMVL
                     TNDVALFIIVPLTAGLHAIGAVPVTRLTLRKPSRSTQALR"
     gene            8819..>9447
                     /locus_tag="VFI43_00215"
     CDS             8819..>9447
                     /locus_tag="VFI43_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7060580.1"
                     /translation="MGNPQNLFLWKISGVSFYHYLLMMLPLALILITILLLFTSFMFN
                     GQKIELREEIDAHRVNEKLFYLSLMLYLPFLILMNMRHTEFATLLVFTIFLIRSPSTL
                     LRIDWGLILILTLMFVDMRLISQHPAVNSLLAGLMLDDSRRLYVAGIALSQLVSNVPT
                     AILLADYSNDWKMIAYAVNIGGSGFILGSLANLIALRLARDGKAWLMFHA"
BASE COUNT         2158 a         2502 c         2500 g         2287 t
ORIGIN      
        1 tcaataacaa taacagtaac aagaaagcaa taacatattc cccttatccg tacgtcgcag
       61 gaacagtgtt cgaccatgcg cagcatggat cgtcgaaaca ctttaaccgc gctcaacttt
      121 cgttggaagc ccccgttccg gacttctcgg cggccaaaag accttttctt ttctccggct
      181 cgtggcactc ctcgcgcgct ggtagtgcct ccacttatca tcgctttttc tcatttcgtt
      241 gcctcatcgc acaaggtacc caaatggcgg ggcatcaacg gcagacatat tattgctatg
      301 ctatctatac ggttaaacca agaggcgccg gtgtctacag gagaatcaca tggaagggat
      361 atttctcaag ttttatgtca gtgaataccg cgagcacaag ggtattctct tgttcgagtg
      421 gttgctggag cgcgccaaga aatgtggctt tcatgggggg acggccctgc gagccgtggc
      481 aggattcgga aggcacggcg tattgcgcga ggaaactttc attgagctcg cggctgacct
      541 tcccgtcgag gtaagttttg tcctgggcac cgaagaggca aagcacttcc tcgatttact
      601 cgaggccgag aacattgacc tgttctatgt caaatttccg gtggagtacg ggtttttgcc
      661 cacggaccag gtagtcgact agcgaatgaa caaatcagag gaactctgaa ctcttgcggt
      721 aatgaaggca gcgagtgcta tcaaaatcag cctccagcgt gcagtgcggg cctgcccgcg
      781 ggctaaccgt ggttccccaa tgtgtttaac ctcctgaacg ctttgtcaat ctggagaatt
      841 ttctccactc tgattctggt ttcatgggtg gccctggctc aagctgaagc tattcccgca
      901 atggatggac aggtcgccat taccgaacag caggccatcg cattattcta ccagcgcaac
      961 ctcagcctca tcgcggcaag cctcaatatc gacgaagccc gggcccggga aatcattgcg
     1021 gctgcaattc ctaaccccgt gctcagcgtc acggtcgccg aacttaatcg caaaatattc
     1081 cgggcggaaa accacgacca gccgttgccc gccgtaatcc cacaggttca acagcttatt
     1141 gaaacggcag gaaaaaggcg attgcgcata gagagtagcg aactggccac tgaagcagtc
     1201 ggttacgact tgcgagacgt tgcgcgaacc ctgaccaacg cagtgcggcg aagctattac
     1261 agcctgatat tggctcaaaa agcccttaaa gtggccaatg acaacttcga gcattatcgc
     1321 gagatcctgc gggtcaatga aatcaggctc aaggtggggg atgtcgccgc gatggatttt
     1381 gtccggatcg aggtcgaagg actaaaggct cagagcgatc aggatcaggc gctggccgcg
     1441 gtaaaacagg cgcgcgccga tctcctgtta ctgctgggct ggcctgaaaa cagcgtggac
     1501 atcgttgctg tagaagcgtg gcccgagata tccccctata ttgctttgtc gagacaggat
     1561 gatctcgtgc tgagggcact ggaacggaga ccggatatgc aagcggcgag gatacggatc
     1621 gcccaggcgc gcaagatggt aacactggcg cagaggcagg tgattccgga tgtaaccata
     1681 agcgcttttt atgatcgtga tcccggcaac ttttttgccc ggtcgggagg cgttggcatc
     1741 agcgtaccca ttcccctgtt ctaccagcag aaaggggaaa tctcaaaggc gcgcgtcacg
     1801 ctcacttccg ccgaactggc cgtcgctcac gcggagcagg caatacgagc ggaagtgctc
     1861 aaggcagctt cggcgtggca gagcgcggac tcgatcgccc gacgtttcga gacttcggtt
     1921 gtcgggcgta ttgaaacatt acggaaatcg caagaacttg cttatcaaaa aggcgcgata
     1981 ggggtgctgg aacttatcga tgcgcaacgc acttaccggg tcgtcatgct cgactattac
     2041 gctgccctcg caaaccgaag taaagcctgg gctgatctgc tcatgggata tggagaagac
     2101 cccgcgcaat ttttgcacaa tccgctaccg ggccgttgac ggccgtcatc aaagcgcgcc
     2161 cgccgctctg tcggctcatc ggagcaagcg aaccatcatc actttgcata ctatgcatgt
     2221 atggggaatg tgagaaatgt ttcatccgat agcaaggttc ggggattccc gttctgcctg
     2281 ttttccccaa aaagactcct gctcctgctt cacagaggtg tggaggcagg aagatcgggc
     2341 agaacctgtc tgctccttgg gatggctgtt ttttgcctgg cggcctgtca gtcgagggat
     2401 agcgaaaaac ccccgcgcgt tcttccctcc catcctgacg aggtcgtact cccgccgaac
     2461 tcacccaagc tccaatatat agaagatatt cgcattgaac cggttcagcg gcccctcatg
     2521 gagccggtcg cagcgaagat cgtgtacgac gaaagccaca ccgttcgtgt tttctcgcct
     2581 attgcgggcc gggtgaccgg cgccattgcc ccgaccggca cggcactgcg cgcaggcgag
     2641 gtgctggtcg aattggatag ccctgaactg ggtcaggcgc aggccatcta ttccgacgct
     2701 ctcgcagacc tcaaccttgc cgaacgcgcc tatcagcgga caaaagagct cgttgataac
     2761 ggaatcgttc cccgaaagga gcttgaccag gcggaggacg cccttagcca cgcccacagc
     2821 gagtctgaac gcgctcgcct caagctggcg aatcttggcg tccgcagtcg gcgtacggac
     2881 aacaggttcg ttctgcatgc gcctatttcc ggaaccgtga cggaacgcaa tatcaatcca
     2941 ggcatggaag tcagggcgga tctgacagca cctctgttcg tcatttcacg tctcgaccag
     3001 ctctgggtgc aaatggatat tttcgaaaaa gacattggtt tgattcacgt aggcgagaaa
     3061 gtattgctgc atgttcccgc gtatccggat aaaaccttta cggcaaacgt tagttatatc
     3121 agccagatag tcgatgaaaa cacgcgggct attaaagtca ggtgcatcct gcccaacccc
     3181 gatttccggc tactgcccgc gatgtatgcc agcgccgagg tacaaagcga tcctggcgat
     3241 cttgctattg tcgttccgct cacggcgtta tttaccgagg atgaatcgga gtgggtatac
     3301 atcaatatcg ggaataacca ttatcaaaag cgtcatgtca aggtgggcct gcggccgaaa
     3361 gatcgcgctg tcattctcga aggtgtgaaa cccggcgagc gcgtggtcgt caagggggcg
     3421 ctgttgctcc gcatagagca ggataacgag gcgcaaagcg gagaacacgc acaatgatca
     3481 accggatcat cgccttctgc ctccagcagc ggctgttgat catcagcact gccatactat
     3541 tgggtgcgat aggcatactc acgttcgaac gcctgcctat caaggcgttt cccgatgtgc
     3601 agaacgtctt tgtgcaggtg gtcacgcaat atccggggca agcgccggaa gaggtggaaa
     3661 aactgatcag cctggccatc gagaaggaga tgaacggcct tccgcacatg atcacgatgc
     3721 gttcggtctc cattttcggc ttatccgtgg tcacgctgat cttcgacgac gaagctgaag
     3781 attatttctc gcggcagttg gtgctcgagc gtttaaaggg cgtcagtctg ccggcaggcg
     3841 taaagccgct tctcggtccc ctgaccacgg gcatcggtga aatttatcgc taccgccttg
     3901 aagcgccagg cgttcccctc gtagaacagc gcgccattca ggactggctg atcgaacgta
     3961 ccctccggtc cgtacagggg gtagcggatg tggtggcttt cggtgggggc atcaagcagt
     4021 atcaggtaca ggtcgattcc aacaagctca ggaattacaa gctgacgcta cccgacgtat
     4081 atgaagcaat cgccggcaat aatgccaata ccggcggcgg ctatatcgat cacggcaacg
     4141 aggcgatggt cgtgcgggga acaggactgc tcaagtcgac tgaagaaatc ggccgggtgg
     4201 cgatcaccac gcaggagggc gttcccgtct tcgtaaaaac cgtggctgat attgtcatcg
     4261 gcccccagcc gcgagtggga atagtcggtt ttgaccagtt cgacgacgtc gtgcagggca
     4321 tcgtcctgct cataaaagga agcaatgcca ctgtggtgct gacgggagtc aaggagaaaa
     4381 tcgattacct caaccgttat ggcctgcctc aaggagtcag aatcattccg atctacgatc
     4441 ggaccgaatt gatacagcac accgtaaata ccgttgagcg caacatggcc gagggcgccg
     4501 tgctgatctt cgctgttctg gtcgtatttc tgcggcggtt ttgggcatcc ctggtcgtga
     4561 ttgtggttgt gccgctatcg ttgctgtttg cttttatcct tatcgacatg aagcatgttt
     4621 ccgccaatct gatctcgctg ggcgcgatcg actttgggat tatcgtggac agtgcggtgg
     4681 tattggttga agcgatcatg gtcaaggtaa ccctggacat gaaccaggga gccagccttg
     4741 cacgcctgcg tcaatccatg ctcagcacaa cggcggagat ggcgcgccct atcctctttt
     4801 caaaggcgat actaatcatc gccttcatcc cgattttcac attccagcgc gtggaagaaa
     4861 aaatcttctc gccaatggcg ttcaccctta ctttcgccct gcttggctcc ctgatcatca
     4921 gcctgacctt ggttcccgtc ctgttgagct atctgctcgg acctcgtctg accgaatcgc
     4981 gcaacccgct tattcactgg atggaaaagc gttacctcaa aatactccag gccgtgctgc
     5041 atcatccgcg caggcttttc ctgggcgcaa gcgttgcgct cgccctgtcg atcgcctcct
     5101 ggccgattat aggcactgaa ttcatgccta aactggatga aggaaacgta tggttgacaa
     5161 ttacattgcc aacccccgtt tctcttgaaa aggccaagga actggaaagg catgtgcgtg
     5221 caagcctcct tgagtttccg gaggcccgct acgtccttac tcaactggga cgcccggaag
     5281 acggcaccga cccaaaaggg ttcaataatc ttgaagtgct catcgatctc acgcccagga
     5341 agacctggcg gtatgccaga aaggacgaac tggtgggggc gatggcaagg cgcctcgcgg
     5401 tattccccgg tttgcagttc aatttttccc aagtgatcca ggacaacgtg gaggaagcca
     5461 tttcgggtgt caagggcgaa attgcgatca agattttcgg cgatgatctg aagattctcc
     5521 agcaaaaggc gaaccagatc actggcattc tccgttcgat cgagggcgca acggatgtgg
     5581 cagcggagca ccaatcggga ttatcgcagc tggttatctc gatagacaga gaaaaaatcg
     5641 cccgtttcgg gattaacata gcggatgtgg aagacgtcat cgaaatggcc gtgggtggca
     5701 aggcggccac gcaaatgctc gagggtgaac ggcggtttga cgtggcagta cgacttaaag
     5761 ctgatgccag ggattcggtg ggcgcaatcg aggatattac ggtactctcc ccgggaggcg
     5821 gccgaatacc tctggcacaa ctggcagaga tcaagaccag tcagggggcg tcacgcatca
     5881 gccgggagaa caacatgcgc ctcatcgcaa tcaaatgcaa cctgattggc cgggaccagg
     5941 gaagcttcgt cgcggaggcg cagaaaaaaa tatcgcgcca ggtgcacctg caaccgggat
     6001 accacatggt ttggagcggg cagttcgaaa accagcagcg ggcgatgaaa cgtctttcct
     6061 ttattgttcc cgtgagcttc gccctgattt tcgtactttt gttctgggct ttccgctcgg
     6121 ttcgaaacgc ttccctgatc gtgatgaacg tgccgttttc cctgatcggc gggctggtag
     6181 ccttgttact gacgggtatt cacctcagcg tctctgctgc tgtaggtttc attgcgctgt
     6241 ttggcatcgc ggtacagaac ggagtgattc tgctatcgca aatcaatgcg ctccggcaag
     6301 aaggggtgcc tataaatgag gccattattc aggggtcagt cagccggctt cgtcctgtag
     6361 tgatgactgc actcatggcg atgctcggat tgctgcctgc tgcgatttca accagcgtgg
     6421 ggtcagaaac agtcaagcct tttgctgtag ttatcatagg cggacttgtc agtgccacca
     6481 ttctgacgct gactctgcta cctgcccttt atcgtaattt tgaggaatgg aatagaacca
     6541 aggtgcttcc atgaaagaaa ttcggaccta cattcagcct ttcatgttat ccagagtcac
     6601 tcaggtattg cttgaaattc cggattttcc tgaatgagcg tgtcagattg cgaaggattc
     6661 gccggagaga agttgatcga cacggatttc accccttatc ttcgcaggaa gcgaatcgag
     6721 atatttgccc cggatgaact tgttaagcga atttttaaca ttgctatgcg cgctgaaaac
     6781 atgcatcagc ggcgaaaatt tttatcctga aggtccagaa aaacggccaa gtcaggtcag
     6841 ggagtgaaaa agacgagcac cccctgaaaa cgtttccaaa gcttgtccat gtcaattctt
     6901 aagggcttga taacgagagc aactaaacgc ccttttcatc ttttatcttg atgagtttgc
     6961 catcttcgcc gacgtcgatc tcaatctcct tgccgtctgg tttttctacc tctgcttcat
     7021 aaacggtaac agttttgccg ctgtcgaaat gaaggactcg aactttctgt attttcgttt
     7081 ccttctcaat ttcctcgatt tttccgccgc ctgcgtgatt gagtatcgtt ttctgaactg
     7141 cgggcggtac atccatccac tgaattctct ccttttctcc ggcaaaagcc gaggttgcga
     7201 ataccaccgc tgcgggcaga acaactgaca tgaataattt cctttccatt tgtgacctcc
     7261 tatttcattc aggacacaca ctcgtcagaa cattctattt acaaaaattc tatttacaaa
     7321 aatgcgttgg tatgataagc gagaattaca aagtaatctg tgcggtaacc ctcttaaact
     7381 gctttgtaaa aggttaagga tggaggggtg atatgcgcac gcttgcttcc cgcgatccac
     7441 tccaattttg aaaaacaacg agaaacttga ggcatattcc atgaaagaaa tccgcgctta
     7501 tattgaaccc ttcatgctgt ccagggtgac tcaggcattg ctcgaaatca cggatttccc
     7561 aggcatgagc gtgtccgact gtgaaggatt cggcggagag aaagtcatcg acatggattt
     7621 cactcctttt ctacatagga agcgggtcga gatattcgcc ccggatgaac tggtggagca
     7681 aatttttaac acccttatgc tcgccgccaa cacgcatcag cgtggcgcag gaaagatcta
     7741 tgttctcgat gtcgagaaaa gcgggcacat tagccggggg acccaaaaag acgaacgttc
     7801 ctgagcaggc tgggacggtt ttcctccttt gttttccgag ggaaaagact ggagaggccg
     7861 tcacgcacgc gcttctctcc tgcccgtttg gggaaagggt cagggagagc acgtcgtggg
     7921 ggagcctgat cgaggcaaaa tcgtccgcgc ttccgctacg tgaatccctt acgatacgtt
     7981 gcggtcgtct tttaactcga taagcctgcc atcttcgccg actttaattt cgactttctt
     8041 tccgccgggc ccgtttacct cagccccata agttgtaacc gtcttgccgt ctttcgttct
     8101 ggtttccttg tcgatttcct cgacttttcc gccacctgcg ttatccgtta tggttttttg
     8161 aactgctgac gggagttgcg accagcgaac tttctcttcc cgttcagcat atgcagaagt
     8221 tgcaatgacc atcgcggccg agagaagtat gcttcttttc attagaattc cccctatacc
     8281 ctatattgtc agagcgaaaa aactttaaaa aatacgggcg atttcgagcg gcgtctgtgt
     8341 agaagcacac agaggaacaa aatcgaaata cgtcgaacac ttgcatggat aaatacgagg
     8401 tcgaggcctt ttcaccgttc cgtgcattac tcaaggatta tcttctgtgg attcttgccg
     8461 ggcttcttgc ggtactttat ttaatgaaac ccttgcggct ttcctcttac ctccgccttg
     8521 tggattggcc caccattgct ggggtggcag gtctgctcgt gcttgccaag ggcatcgaat
     8581 tctcaggata cctgcatcga ctgggcaagc gcgttataga agcaatgcct acgggcaggt
     8641 cgctcgcaac cttgcttgta ctggctgccg cgttgctatc gatggtactt accaatgacg
     8701 tcgccttgtt catcattgtg cccttgactg ctggcttgca cgcgatcgga gcggtgcccg
     8761 tgacccggct gacattgcgg aagccctcgc ggtcaacgca ggctcttcgc tgactccaat
     8821 cggtaaccct caaaatctgt tcctctggaa aatatcggga gtatcgtttt atcactatct
     8881 cctgatgatg ctaccgctcg ccttgatcct tatcactatc cttctattgt ttacctcatt
     8941 tatgttcaac ggacagaaga ttgaattgag agaagaaata gacgctcatc gtgtcaatga
     9001 aaagctattc tacttatcac tgatgctcta tctgccgttt ctgatcctta tgaatatgcg
     9061 ccatacggag ttcgcgacgc ttctggtatt cacgattttc ctgattcgca gtcccagcac
     9121 gcttttgcgc attgactggg gcctcatcct gatactcact ctcatgtttg tcgacatgcg
     9181 gctgatttcc cagcatccgg ctgtcaattc attgcttgca ggactgatgc tggacgactc
     9241 tcgccggctt tatgtcgccg gaattgcgct ttcacaactt gtcagcaacg tccccactgc
     9301 cattttactg gcagactact ccaatgactg gaagatgatt gcttacgcag tcaatatcgg
     9361 cgggtcggga ttcatattag gctcgctcgc gaatctcatt gcgttgcgcc tcgcaaggga
     9421 tggtaaagcc tggctgatgt ttcatgc
//
LOCUS       DASSHJ010000004         7183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_320840, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000004 DASSHJ010000000
VERSION     DASSHJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320840"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..790)
                     /locus_tag="VFI43_00220"
     CDS             complement(80..790)
                     /locus_tag="VFI43_00220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET7060581.1"
                     /translation="MKPVAIFRHFPTEGPGYLATFLDSHSIPWQLIKIDSGGWVPSTP
                     DAFGGLVFMGGPMSVNDDLPWIPPVLSLIRHAVEDGIPVLGHCLGGQLMSKALGGVVE
                     PSPIKEIGWGEVYLADNPVAPRWVGGKAIFEVFHWHGETFTLPEGSTLLFSSPYCQNQ
                     AFALDNHLGLQCHVEMTEEMVITWCKTGAREIAASAGPGVEPVAAIQMRLVERVSALN
                     KIADKLYEKWILGLKSEA"
     gene            complement(903..1487)
                     /locus_tag="VFI43_00225"
     CDS             complement(903..1487)
                     /locus_tag="VFI43_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET7060582.1"
                     /translation="MVIVQSPIIPLMHLPKSPSTSANDKARAFSWKRFFVHCSIVTTA
                     VSLGVMSWFIAKPTATRIHQTVPGERITVAATPDIDISLDADSLVTVSNTEPPWIELL
                     KGSAYFDIRNKNPDKLHVKVGTAFIKDLGTRFSIRRRADGGSVAVAEGQVEIQVESGN
                     YLVSSREGADFDDRRVTGHRMISEADIAPWRTRK"
     gene            complement(1616..2035)
                     /locus_tag="VFI43_00230"
     CDS             complement(1616..2035)
                     /locus_tag="VFI43_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7060583.1"
                     /translation="MAFGSMDNGSRQMPTSEINVVPLVDVMLVLLVIFIITAPLLTHS
                     VKIDLPKAASSPNITQPDHIEFALRDDSTLYWNGEPVSMDQLAPRFSTSVKENPDIEL
                     HIRADRHVHYENVARVMSIAAKAGLVKIGFVTDPSEK"
     gene            complement(2035..2787)
                     /locus_tag="VFI43_00235"
     CDS             complement(2035..2787)
                     /locus_tag="VFI43_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7060584.1"
                     /translation="MEKGLGFSHFLTQLDAVGTAVLVLLLTLSIASWYLILTKGLANF
                     LAARRADAFLKRFWSAGSLQEVNSTLKEGSGDNAFAHLAQLAMNAAADSEKHGLQKLA
                     AAGGTSEFVTRVLRNGLDQEAAKVEDGLTIIASAGSAAPYIGLFGTVWGIYHALVQIG
                     LSGQGTLDKVAGPVGEALIMTALGLAVAIPAVLAYNTFVRRNRIWLARLDAFAHDLFV
                     LVTVGSKTNSSAGDERPLRVVSPVLAPNERRS"
     gene            complement(2792..3445)
                     /locus_tag="VFI43_00240"
     CDS             complement(2792..3445)
                     /locus_tag="VFI43_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET7060585.1"
                     /translation="MVFNRNPTFLPGLMLVIAIHAALLYFLFKQQFIPPPKQLETLLV
                     NFIPAARPKDEPKPQPPAPPKSEPVKKKPQSRQIVSETPVVSENEPVALSFQPEPEPQ
                     PVAVAEPSPAPAPQMPAGPVTLSSELSVACPQLNAPTYPALSRRLGEEGKLVLRVELD
                     EKGLVSQAQIVNSSGFKRLDEAAMTAVKTWRCTPPVRDGQPVRAVALQPFNFVLQGD"
     gene            complement(3448..4023)
                     /locus_tag="VFI43_00245"
     CDS             complement(3448..4023)
                     /locus_tag="VFI43_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060586.1"
                     /translation="MDTSPMRFILSALMLKTTTFSGRNNAFFLTRGLLFVLMLASAAF
                     ASAAEVIRPFTPGSLAQVLAQREGKPFILVLWSLECQYCPTELKTLSELKHVHPKMDI
                     VLVATDEVSDIPQLLSRTESYGISKVEQWVFAEDMPERLRHEIDGRWYGEVPRTYFYD
                     RKHQREVKTGLVNKKFFEDWLARNAVSGAGN"
     gene            4503..5072
                     /locus_tag="VFI43_00250"
     CDS             4503..5072
                     /locus_tag="VFI43_00250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7060587.1"
                     /translation="MATRQEFSKFLIETERRAYKQALFAVHDEPLALDIVQDSMTKLV
                     EKYADKPLAELPLLFQRILQNTIRDYYRRQKTRSLWTTLFSSLIPRNESENEDYDPLE
                     TLQSEGSSNADDPYRQLEQSELIRLIEKTIEMLPPRQREAFLLRYWEEMDVTETAKVM
                     GCSEGSVKTHCSRATHTLALALKKEGIEP"
     gene            5069..5449
                     /locus_tag="VFI43_00255"
     CDS             5069..5449
                     /locus_tag="VFI43_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HET7060588.1"
                     /translation="MTEDEIGRKATRLLDRDLDSMKQSTLNRLQAARRAALENYEAAE
                     EMVSVPRTASAGAHRNDHPGIAVRKLVSILALLFAITAAVYWQNFQQSDENEDIDIML
                     LLDDVPVNAYLDDEFDAWLEGSGQ"
     gene            5425..5814
                     /locus_tag="VFI43_00260"
     CDS             5425..5814
                     /locus_tag="VFI43_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HET7060589.1"
                     /translation="MARRVRTVAFWLCLVVSAQVLAESPPPPWEQLNRQQKEVLAPLA
                     QEWNSMDPVKRKKWLGIAKRYPGMTEAEQHRAQLQMRDWYSLTPEQREIVREKYKTIK
                     KLPPDKRQEIKQKWRGYGHHAEDQAGQ"
     gene            6127..6555
                     /locus_tag="VFI43_00265"
     CDS             6127..6555
                     /locus_tag="VFI43_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7060590.1"
                     /translation="MTQASTPGFLRRLLSMLYESLMLVAVLFIASFIFHLLFQDTSSV
                     LFRPAFQLYLLLIAGIYFIWFWTHGGQTLPMQTWKLRVVTADGHQLHFKQALARYLFA
                     VIGIFLLGCGIIWALFDRDHQFLHDRLAGTRIEGASVKKG"
     gene            complement(6552..>7183)
                     /locus_tag="VFI43_00270"
     CDS             complement(6552..>7183)
                     /locus_tag="VFI43_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7060591.1"
                     /translation="FNRKGSSAFSGRIGERVAAEGVTVVDDGTLARRRGSLNVDDEGN
                     PTQCTVLIENGVLKGYLQDSLNARLMNVPVTGNARRESFAHVPMPRMTNTYMLNGDKT
                     PEEIIASVKHGLYAVNFGGGQVDITSGKFVFSAAEAYMIENGKISYPVKGATLIGNGP
                     DVLTRVSMIGNDMSLDPGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTA"
BASE COUNT         1697 a         1935 c         1867 g         1684 t
ORIGIN      
        1 gactgagaat caacccttgt ttacccgatg caggctgttc aacgacgggc aaggcaagac
       61 tcagagaaga gcggtagtct caggcttcgc tcttaagccc caatatccat ttttcgtaca
      121 acttatcggc gattttgttc aacgcggata ctcgctcaac cagtctcatc tgtatcgccg
      181 caaccggttc aactcccggc ccagcactcg ctgcgatttc ccttgccccc gttttgcacc
      241 acgttatgac catttcttca gtcatctcca cgtggcattg caaacccaag tgattatcca
      301 gtgcgaacgc ctggttttgg caataggggc tggaaaaaag aagagttgaa ccttccggca
      361 aggtgaaagt ttctccatgc caatgaaaaa cttcaaagat cgccttgccc ccaacccacc
      421 tcggggcgac agggttatcg gcgagataaa cctcacccca gccgatttcc ttgattgggc
      481 tcggctccac cactcctccc agcgcttttg acatcaattg tccgccgaga caatgaccga
      541 gcacggggat gccatcctcc acagcatggc gaatcaatga aagtaccggt ggaatccagg
      601 gtaagtcatc attgacgctc atgggaccgc ccatgaacac caggccgccg aacgcgtccg
      661 gcgttgaagg aacccatccg ccggagtcga tctttatgag ctgccaggga atggaatgag
      721 aatcaagaaa ggtagcgaga tatcccggac cttcggtcgg gaagtgcctg aaaatggcaa
      781 ccggcttcat gtcggctttt gattaatttg aggggcgccg ggatccctgg aacagcaacc
      841 cccgacagga gccggaatgg aaaataacca taccttcgct ccgttttacc agctcgccag
      901 ttctatttcc tggtccgcca aggcgcaata tctgcttcag aaatcattct gtgtccggtg
      961 acccgcctgt cgtcgaaatc cgccccctcg cgcgagctga ccaagtaatt tccactctct
     1021 acctggatct cgacctggcc ttcggctacg gctacgctgc caccgtccgc ccgcctcctg
     1081 atactgaagc gcgtgcccag atcctttatg aaggctgtgc ctaccttgac atggagtttg
     1141 tccggattct tgttcctgat atcgaaatac gcactgcctt tcagaagctc gatccatgga
     1201 ggctccgtat tgctgacggt taccaagcta tcagcatcca ggctgatatc gatatcaggg
     1261 gttgcggcaa ctgtgatgcg ctcccccggc acagtttgat gtattcgtgt tgccgtcggt
     1321 ttggcgataa accatgacat cacacccagc gatacagcgg tagtgacaat tgagcaatgc
     1381 acaaaaaaac gcttccagct gaaggcgcgc gctttgtcgt ttgcggaagt ggaaggagat
     1441 ttgggcaaat gcatcagcgg aatgatggga gattgtacga tcaccatctt atatcttata
     1501 cgatcagaca atcgccagct ctccgggttt ccgcaaaaag cctttctcct gacggaaatc
     1561 aggtggattt taccactgca taagagacct tcaggaaagc tattctagag ggagattact
     1621 tctccgaagg atcagtaaca aatcctattt ttaccaatcc cgccttagcc gctatcgaca
     1681 tcactcgcgc cacattttca taatgaacat gccggtcggc gcgaatgtgc agttcgatat
     1741 ccggattttc cttgactgat gttgaaaaac gaggcgccag ctgatccatg ctgaccggtt
     1801 cgccattcca gtaaagtgtg gaatcatcgc ggagagcgaa ttcgatgtga tcaggttgag
     1861 taatatttgg actgctcgct gccttcggca gatcgatttt gactgaatgc gtaagcaagg
     1921 gggccgtgat gataaatatc actaacagca ccagcatgac gtcaaccagc gggaccacgt
     1981 tgatttcgga tgtcggcatc tgacgacttc cgttatccat gctgccaaag gccatcatga
     2041 cctcctttca ttaggagcca ggacagggga aaccactcgc agcggacgct catctcccgc
     2101 tgaactattg gtctttgatc cgacagtcac caatacgaac aagtcgtgcg caaaggcgtc
     2161 cagccgtgcc agccagatgc ggttgcggcg cacaaacgtg ttgtatgcga ggaccgcagg
     2221 gatcgccacc gccaagccca gggctgtcat gatcaaagct tcacccacgg gacctgcgac
     2281 tttatccagc gtcccctggc ctgaaaggcc aatttgcacg agcgcatgat aaatacccca
     2341 gaccgttccg aataatccta tataaggcgc cgcagaaccc gcggaggcaa ttatggtaag
     2401 gccatcctca accttggctg cctcctggtc aagaccattg cgcaagacac gggtaacaaa
     2461 ctcgcttgtt ccaccagctg ccgcaagctt ctgcaatcca tgtttttcgg aatccgctgc
     2521 cgcgttcatc gcgagttgcg ccagatgcgc gaacgcgtta tcgcctgaac cttccttgag
     2581 tgtcgagttt acttcctgca gagaccccgc gctccagaaa cgcttgagga aggcatctgc
     2641 gcgtcgggcg gcaagaaaat tagcaagacc tttggtgagt atgagatacc agcttgctat
     2701 tgaaagtgtc agcaatagca ccagtaccgc tgttccgacc gcatcgagct gggtcagaaa
     2761 atgagagaat ccaagacctt tttccatcag atcaatctcc ttggaggaca aaattgaagg
     2821 gttgcagcgc gacggctctc acgggctgac cgtcacgcac ggggggagta cagcgccagg
     2881 ttttgacggc agtcatggca gcttcatcaa ggcgcttgaa accgctgcta tttacgatct
     2941 gagcctggct gacaaggcct ttttcgtcca gttccacgcg cagcaccagc ttgccttctt
     3001 cgcccaaccg ccgcgacagc gccgggtagg tgggcgcgtt cagttgcggg caggcaacgg
     3061 agagttcgga agaaagcgta acagggcctg ccggcatttg aggagcaggg gcaggagatg
     3121 gttcggcaac cgcgacaggt tgcggctcgg gttcaggctg gaatgacaat gctacgggct
     3181 cgttttctga gaccaccggc gtttccgaga caatctggcg ggattgcggc ttttttttca
     3241 ccggctccga cttgggcggt gcaggcggct ggggcttggg ctcatccttt ggccgggcgg
     3301 caggaatgaa attgaccagc agggtttcaa gctgcttggg tggagggatg aactgctgct
     3361 tgaacagaaa ataaagcaag gcggcatgaa tcgcgatgac aagcatcagg cctggcagga
     3421 aggttgggtt gcggttaaag accatgttca atttcccgct cccgacacag catttcgagc
     3481 cagccagtcc tcaaagaact ttttattgac caggccggtc ttcacttccc gctgatgctt
     3541 gcgatcataa aaataggtcc gcggaacctc cccataccat cggccatcga tttcatgtcg
     3601 caagcgctcg ggcatatcct cggcgaagac ccattgctcc actttgctta tcccgtagct
     3661 ttcggtgcgg gatagtagct gggggatatc acttacttca tcggttgcga ccagtacgat
     3721 atccatcttc gggtggacat gcttgagctc gctcagcgtt ttcagctcgg tcgggcaata
     3781 ctgacattcg agcgaccata aaaccaggat aaaaggcttg ccttcgcgct gggcgagaac
     3841 ctgagcgaga cttcccggcg taaacgggcg aatgacttcc gctgcggaag cgaaggccgc
     3901 cgaggctagc atcagcacga acagcaaacc gcgcgttaga aagaaggcgt tgttccgccc
     3961 gctgaaagtc gttgttttca gcataagagc gctcagtatg aagcgcatag gtgaggtatc
     4021 catgggattt ccgaacattc tgccgatgtg ggaccaagag ccggtactga tattgtagtt
     4081 gtaaatcgaa ttaagaatca ctcgcattag aattttaaca ctaacctcca tctgatacat
     4141 agtgtctgac cgaattctaa agtttttatc cgatggactc ggtcatcccc accctggagg
     4201 gacgtggatg cccataatgt tagggaggaa aagaggtttt tacaatgcga caaattgtcg
     4261 caggtgaaga ctacttaagg acgctcctct acgactttgc ttacgtagtt ttggttcggc
     4321 atcaagggca atcccatcgc ccggagaata gttgtgtgct cgcgtgctca cgttccggag
     4381 agctgatcgc cccgttcagc aggtgctttc tggccaaggg aaaaaatttg ttaatattgc
     4441 tgggttaatg ctcttttgtc gtttttcgct cctcagtttt tattctcata taggcaatcg
     4501 ccctggctac tcgacaggaa ttctccaaat ttctgattga aaccgaacgg cgcgcctata
     4561 agcaggcgtt gtttgcggtg cacgatgagc cgttggcact ggacatcgtt caggattcga
     4621 tgacgaagct tgttgagaaa tatgctgaca agccgcttgc agaattgcct ctgctgttcc
     4681 agcgaattct ccagaatacg atacgcgatt attaccggcg gcaaaaaacc cgttcgttat
     4741 ggacaaccct tttttcatcg cttattcctc gtaatgagag cgaaaatgaa gattatgatc
     4801 ctctggaaac tttgcaatca gaaggatcgt cgaatgcaga cgacccgtac aggcagctgg
     4861 aacaatcgga acttatcagg ctgatcgaaa aaaccataga gatgcttccc ccgcgtcaac
     4921 gagaagcctt tcttctgcgt tactgggaag agatggatgt tacggaaact gcaaaggtga
     4981 tgggatgctc cgagggtagc gtgaaaaccc actgctcccg ggcaactcac acccttgcct
     5041 tggcgctgaa aaaggaaggg attgagccat gactgaagac gaaatcggaa gaaaagcgac
     5101 gcggcttctc gatcgggatc tggacagcat gaagcagagc acgttaaatc gccttcaagc
     5161 tgcccggagg gccgcgttgg agaattatga ggccgcggaa gagatggtga gcgtgccgcg
     5221 gacggcctcc gctggcgctc atcgcaacga tcatcccggt atcgcggtcc gcaagctggt
     5281 atccatattg gccttgctat ttgcaattac ggcagcggtc tattggcaaa attttcaaca
     5341 aagcgatgaa aacgaggaca tcgatatcat gctccttctc gatgacgtac ctgtgaatgc
     5401 ttatcttgat gacgaatttg atgcatggct agaagggtcc ggacagtagc attctggctg
     5461 tgccttgttg tatccgctca ggttctagct gagtcgcccc cgccgccatg ggagcaattg
     5521 aaccgccagc aaaaagaggt gctggccccg cttgcccagg aatggaacag tatggatccc
     5581 gtaaaaagaa aaaaatggct gggcatcgcc aaacgctatc ccgggatgac tgaagccgag
     5641 cagcatcgcg cccaactgca aatgcgcgat tggtattcgc tgaccccgga gcagcgggag
     5701 atcgtgcgcg aaaaatataa aacgataaaa aaactacccc cggataaacg gcaggaaatc
     5761 aagcagaagt ggcgcggcta tggtcaccac gctgaggacc aggccgggca atgagctatc
     5821 tataggacta taataatgcc cgcaagcgcc tcctgcacac ttgtccgtgc atgggattgc
     5881 aaggaggttg gccaacggat ggcggggaag cgtaaccatt ggttatgccg gaatggttat
     5941 agtttcgccc cacttcactc atctgccgat actgtcagat gccgatattg ataggttcgg
     6001 aatcgagcct ctacccatcc tacttcttta ggtgtagttt ctcacaacga acgttgctca
     6061 tccgctcgct cccgcggatc atttcgcatc ggaccattta aagtcgaaat tagcaaatcc
     6121 ttctgaatga cccaagcttc tacgccagga ttcctgcggc ggctgctcag tatgctttat
     6181 gaatcgctga tgctggttgc cgttcttttt atcgcgagct ttatttttca cctcttgttt
     6241 caggatacaa gctcggttct ctttcgaccc gcatttcagc tttatcttct gctcattgcc
     6301 ggaatttatt tcatctggtt ctggacgcac ggcggccaga cattacccat gcaaacatgg
     6361 aaacttaggg tggttacggc agatgggcat caactgcatt tcaagcaggc gctggctcgc
     6421 tatctgtttg cagtcatcgg aatcttcctg cttggctgcg gaataatctg ggcgttattc
     6481 gatcgcgacc accaatttct tcatgacagg ctggctggca cccgtatcga gggtgccagc
     6541 gtcaagaagg gctaggcagt tcctccaacc gtcagtccgt cgatacgaag ggtcggttgt
     6601 ccaacgccca ctggcacgct ttgaccttcc ttgccgcatg tccccacgcc agggtcaagc
     6661 gacatatcat tgccgatcat cgatacacgg gtcaacacgt ccgggccgtt accgatgagc
     6721 gtcgcgcctt tcacggggta ggaaattttc ccgttttcga tcatatatgc ctccgcggcg
     6781 gaaaagacaa attttccgct cgtaatatcg acctggccac ccccgaaatt taccgcgtat
     6841 agtccatgct tgaccgaggc aatgatttct tccggtgtct tgtccccgtt cagcatataa
     6901 gtatttgtca tgcgtggcat gggtacatgg gcaaacgatt cccgcctcgc attgccggtg
     6961 accggaacgt tcatcagccg ggcgttcagg ctgtcctgga gatagccctt gagaacgcca
     7021 ttttcgatca gtaccgtgca ctgggtcgga ttcccctcgt catccacgtt cagtgaacca
     7081 cgtcgccgtg caagcgtgcc atcatccact acagttacgc cttctgccgc tacacgctcg
     7141 ccgatccgtc cggaaaatgc agaacttccc ttacggttga aat
//
LOCUS       DASSHJ010000005         7418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_320654, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000005 DASSHJ010000000
VERSION     DASSHJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320654"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..436
                     /locus_tag="VFI43_00275"
     CDS             239..436
                     /locus_tag="VFI43_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HET7060592.1"
                     /translation="MLKIIAVLFLLFILSSLGSALYYVIKDKGQGTRAVRSLTFRIGF
                     SILLFIFFMAGTYFGFIPLTK"
     gene            complement(459..1325)
                     /locus_tag="VFI43_00280"
     CDS             complement(459..1325)
                     /locus_tag="VFI43_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7060593.1"
                     /translation="MSSHEAGHGHGHYYVPAPSTYPITGSIALLFMGFGAAFTVNKIP
                     LGYGLLAFGFAILIYMMFGWFGTVARESEGGKYSKQVDASFRWGMSWFIFTEVMFFAA
                     FFGALFYMRVFSVPWLSDLEHKLLWPDFAGGWPVSGPGIEDKFAPMGAWGLPAINTLI
                     LLTSGVTVTWAHWALKLNKRGQLKLGLFLTFALGFLFVGLQAYEYGHAYSDLNLKLTS
                     GAYGASFYMLTGFHGFHVTIGSIMLFVIFCRVMAGHFKPENHFGFEGVAWYWHFVDVV
                     WLGLFIFVYWLV"
     gene            complement(1358..1564)
                     /locus_tag="VFI43_00285"
     CDS             complement(1358..1564)
                     /locus_tag="VFI43_00285"
                     /inference="COORDINATES: protein motif:HMM:NF022622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HET7060594.1"
                     /translation="MESDGKPARGTFVQAMKAVSWAFFGIRKQSDLDNDASTLTLGQV
                     IVAGLVGAGLFVAAIVTMVKLVTS"
     gene            complement(1578..2120)
                     /locus_tag="VFI43_00290"
     CDS             complement(1578..2120)
                     /locus_tag="VFI43_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7060595.1"
                     /translation="MNLVQANSVVMKKLLIFTVVMFGFGFALVPFYEKICEVTGINNL
                     LQADTIAANTQVDTSRWVTVELDANIRGLPWQFKPLQSTIRVHPGELNQVMYEVRNTT
                     DREISGQAIPSYSPQLLGKHLKKLECFCFSKQVLKPNEVKQMPVQFMIEPGLPETYDT
                     VTISYTFFELGAKPAASPDS"
     gene            complement(2264..3850)
                     /gene="ctaD"
                     /locus_tag="VFI43_00295"
     CDS             complement(2264..3850)
                     /gene="ctaD"
                     /locus_tag="VFI43_00295"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7060596.1"
                     /translation="MAAVHEHDVAHAHDDHHPSGISRWICATNHKDIGTMYLWFAFTM
                     FLTGGVMALTIRAELFQPGIQIVNPEFFNQLTTMHGLIMVFGAIMPAFVGFANWQVPM
                     MIGAPDMAFARMNNWSFWLLPPAAMLLVGSFFVPGGAPAGGWTIYPPLSVQMGMGMDM
                     AIFALHIMGASSIMGSINIITTILNMRAPGMTLMKMPLFVWTWLITAYLLVLVMPVLA
                     GAVTMLLTDRNFGTNFFNAAGGGDPVMFQHIFWFFGHPEVYIMILPAFGIVSEIIPTF
                     ARKPLFGYSSMVYATASIAILSCIVWAHHMFTAGMPVTGQLFFMYATMLIAVPTGVKV
                     FNWTATMWRGSMTFETPMLFAIGFIFLFTLGGFSGVICAIVPIDIQVQDTYYVIAHFH
                     YVLVSGALFSIFGGIYYWLPKWTGHMYDESLGKLHFWLSFFFFNLAFFVQHFLGLAGM
                     PRRIPDYALQFADFNMISTIGAFGFGLSQLLFLYVVLKCIRGGAKAPAKPWEGAKTLE
                     WTLPSPAPYHSFETPPVFKG"
     gene            complement(3867..5012)
                     /gene="coxB"
                     /locus_tag="VFI43_00300"
     CDS             complement(3867..5012)
                     /gene="coxB"
                     /locus_tag="VFI43_00300"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7060597.1"
                     /translation="MSSKASTVAWGVMWGLAAGLYAGLALAAADPYQLNFPEPKSVIA
                     RDIYDQHTMLLWICLVIFIGVFGTMFYSMLKHRKSQGYKAANFHHSTTVEIIWTIIPF
                     VILMGMAYPATKTIIAMKDTSNPDITIKTTGYQWKWGYDYITGEGEGISFMSALATPR
                     GQIDNSQPKGENYLLEVDNRLVVPVGKKVRILLTANDVIHAWWVPALGAKQDAIPGYV
                     RDTWFTADSPGTYRGQCAELCGKEHGFMPIVVEAVEPAKYTQWVAEQKKKTAVAAVDT
                     NKVFSADELKAEGEKVYTANCVACHQANGKGTPPAFPPLEGSKIANGPKADHINIVVN
                     GKTGTAMAPFKHLSDVQIAAVVTYERNSWGNKAGDVVQPSEINEFRK"
     gene            complement(5957..6388)
                     /locus_tag="VFI43_00305"
     CDS             complement(5957..6388)
                     /locus_tag="VFI43_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HET7060598.1"
                     /translation="MFNPSREQARQFFFDTWRKYREREVLSAMENMVLDVILMHPEYQ
                     TVLEDPERYQDKDYLPEMGDINPFLHMSMHVAIDEQLSIDQPSGIRDRFERLLSKTGE
                     KHAAMHQVMECLAEMIWQAQRNKTDVDATVYFACLDRSIYS"
     gene            complement(6451..7089)
                     /gene="nth"
                     /locus_tag="VFI43_00310"
     CDS             complement(6451..7089)
                     /gene="nth"
                     /locus_tag="VFI43_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7060599.1"
                     /translation="MNAQKITEIFERFKAANPHPATELEYSSPFELLVAVILSAQATD
                     KGVNIATRKLFSKANTPEKILALGESGLQEFIKSIGLYKTKAKNILATCALLIQDHGG
                     EVPRTREELERLPGVGRKTASVVLNTAFGEIAIAVDTHIFRVCNRTGIARGKTVLEVE
                     RKLLKCVPPQFQHDAHHWLILHGRYVCVARKPKCPVCLINDLCEYGEKTQTL"
     gene            complement(7140..>7418)
                     /locus_tag="VFI43_00315"
     CDS             complement(7140..>7418)
                     /locus_tag="VFI43_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060600.1"
                     /translation="ARARYQFRLKRLDRDRREKEEAKEDHQKEKAEESLRFLEIESQK
                     FELDLKETPVKERSAKESQDRKRAMIQAALKRAMAARAQQLGAEDRTE"
BASE COUNT         1740 a         2038 c         1826 g         1814 t
ORIGIN      
        1 aggaggaatt gttaccgcag gctgccggga gagcacgtcg agctgttcct gccgcgactc
       61 cttttccctg gcacgggaga gctgccagtt gccaagctgc accatgatcg ttacgacaat
      121 caccgcaact attgttgacc ataatcgtgg cgtaaagcgc cagcctgaaa tagtcattgg
      181 atagagtgcg gaaactcagc taaactttcc gttaggcccc actaaatagt gaaacaaaat
      241 gttaaaaatt attgcggtgc tttttctgct tttcatactt tccagcctgg ggtctgccct
      301 gtattacgtc atcaaggata aaggccaggg aacgcgagcc gtgagatccc tgaccttccg
      361 tatcggtttc tcgatactcc ttttcatctt ttttatggct ggaacgtact tcggctttat
      421 cccactgacg aaataaacga actgcgttta tccggccgct ataccagcca gtagacgaag
      481 atgaacagcc caagccacac cacgtccacg aagtgccaat accatgccac accttcgaag
      541 ccgaaatggt tttcgggctt gaaatgtccc gccattaccc gacagaaaat cacgaacagc
      601 atgatggacc caatcgttac atgaaagccg tgaaacccgg ttaacatgta aaaggacgcg
      661 ccataggcac cactcgtgag cttgaggttg agatcagaat aggcgtggcc atattcgtaa
      721 gcctgcaagc ctacaaagag aaatccgagg gcaaaggtca ggaacaatcc aagcttgagt
      781 tgcccgcgct tgttgagctt gagcgcccag tgagcccatg tcaccgtgac gcccgaggtc
      841 agcagtatca gggtattgat agccggaaga ccccatgccc ccattggagc aaatttatcc
      901 tcgattccag gtccggaaac aggccatcct cccgcgaaat caggccacaa caatttatgc
      961 tcgagatcgg acagccaggg aactgaaaac acccgcatgt agaacaatgc gccgaagaag
     1021 gcggcaaaga acatgacctc tgtgaaaatg aaccagctca tgccccagcg aaacgaagcg
     1081 tccacctgct tgctgtactt gccgccttcg ctttcgcgag cgacggtgcc gaaccatcca
     1141 aacatcatgt atatgagaat ggcgaatccg aaagcgagca acccataacc aagcggaatc
     1201 ttgtttactg tgaatgctgc cccgaaaccc atgaataata gtgcgattga cccagtaata
     1261 ggataggtcg atggtgctgg aacataataa tgaccgtgac catgccctgc ttcgtggcta
     1321 ctcatgctct ttctcccact gtaatttaat caatttttta acttgtcacc aacttgacca
     1381 ttgtaactat cgccgcgaca aacagcccgg cgccaacgag gccggctaca atcacttgtc
     1441 ccagtgttaa tgtactagca tcgttgtcga gatcgctttg tttccttatg ccaaagaatg
     1501 cccacgatac ggctttcatg gcttgcacaa acgtcccccg cgctggtttc ccatcggatt
     1561 ccatcaatct cgtttcctta tgaatcgggg ctcgcggccg gtttggcccc gagctcgaag
     1621 aacgtatagg atatggttac cgtatcgtag gtttcgggca atccgggctc gatcatgaat
     1681 tgaacaggca tctgcttgac ctcattgggc ttgagcacct gcttgctgaa acagaagcat
     1741 tccagttttt tcagatgctt gcccaggagt tgaggactat agctcggtat cgcctgcccg
     1801 ctgatttcgc ggtctgttgt atttctgact tcgtacatca cctggttcaa ttcccctgga
     1861 tgcacacgaa ttgtcgactg gaggggcttg aactgccagg gcagcccgcg gatgttcgca
     1921 tccagctcca cggtcaccca ccggctcgta tcgacctgcg tgttcgctgc gatcgtatcc
     1981 gcctgcaaca agttgttgat tccggtgact tcgcagattt tttcgtaaaa cggtaccaac
     2041 gcgaaaccga aaccaaacat cactactgta aaaatcagta gcttcttcat tacaacggaa
     2101 ttagcctgta ccaggttcat tacgagtttt ttacgataac cgttataaaa agtgctatta
     2161 cgatagccag caaaatcagg gcggttttta ttgctccgcg cctttgttcg gcacttcttt
     2221 gctcgtcatt tgctggcatc ccgaagccta ttcgtggact tgattaaccc ttgaatacgg
     2281 gaggcgtttc gaagctgtgg tacggagccg gtgacggcag cgtccattcg agcgtctttg
     2341 ccccttccca tggtttcgcc ggcgctttcg ctccaccgcg gatgcacttg agcaccacgt
     2401 agaggaacag caattggcta agcccgaacc cgaacgcgcc gatggtggaa atcatgttga
     2461 aatcggcaaa ttgcagggca tagtcgggaa tacggcgcgg catgcctgcc aggccaagga
     2521 agtgctggac gaagaacgca aggttgaaga agaagaacga caaccagaag tgcagcttgc
     2581 cgagcgattc gtcatacata tgcccggtcc acttcggcag ccagtagtat atgccgccaa
     2641 atatggagaa caacgctccg gatacgagca cataatggaa gtgtgcgata acgtagtagg
     2701 tgtcctgtac ctggatgtcg atcggtacaa ttgcgcatat aacgccgctg aagccgccga
     2761 gggtaaacag gaatatgaac ccgattgcaa acagcattgg tgtttcgaag gtcatggagc
     2821 cgcgccacat tgttgcggtc cagttgaaca ccttcactcc tgtcggaacc gcgatcagca
     2881 tggttgcgta catgaagaaa agctggccgg tcaccggcat gccggcggtg aacatgtggt
     2941 gcgcccagac gatgcaggac aggatcgcga tagatgctgt cgcataaacc atcgacgagt
     3001 agccaaacag cggcttgcgc gcgaatgtcg ggatgatttc cgacacgatt ccaaatgcag
     3061 gcaaaatcat gatgtagact tctgggtggc cgaagaacca gaagatatgc tggaacatca
     3121 ccgggtcccc gccgcctgcc gcattaaaga agttggtgcc gaaatttcgg tcggtcagca
     3181 gcatcgtaac cgcgcccgcc agcactggca tcaccagcac gaggagatag gcggtgatca
     3241 accaggtcca tacgaacagc ggcattttca tcagcgtcat gcccggcgcc cgcatgttca
     3301 gaatggtggt gatgatgttg atcgagccca tgatcgatga tgcgcccatg atatggagag
     3361 caaagatggc catatccatt cccatgccca tctgcaccga cagtggcgga taaattgtcc
     3421 agccgcccgc aggcgcgccc cccggcacaa agaatgagcc gacaagaagc atcgcggcag
     3481 ggggcagcag ccagaaactc cagttgttca ttcgggcgaa tgccatatcg ggcgcaccga
     3541 tcatcatggg gacttgccag ttggcaaacc cgacgaaggc cggcatgatg gccccgaaca
     3601 ccatgataag gccatgcatg gtggtcaatt gattgaaaaa ctcgggattg acgatctgaa
     3661 tgcccggctg gaacagttcc gcgcgtatgg ttagcgccat cacgcccccg gtcaggaaca
     3721 tggtgaacgc aaaccacaga tacatcgtgc cgatgtcctt gtggttggtg gcgcaaatcc
     3781 agcgggaaat cccgctggga tggtggtcgt cgtgtgcgtg tgcaacgtcg tgttcgtgta
     3841 ctgctgccat ggtttcctcc ttgatgctat tttctgaatt cgttgatctc tgatggctgt
     3901 actacgtctc ccgccttgtt gccccaggaa ttccgttcgt aggtgaccac cgccgcgatt
     3961 tgtacgtccg acagatgctt gaatggagcc atcgcggtgc cagtcttgcc gttcactacg
     4021 atgttgatat gatccgcctt gggtccgttg gcgatctttg atccttcaag cggggggaag
     4081 gcgggaggag tgcctttgcc atttgcctgg tggcatgcca cacagttggc agtatatacc
     4141 ttctcgccct cagccttgag ctcgtccgca ctgaacacct tgttggtgtc gacggccgct
     4201 acagcggttt tcttcttctg ctcggccacc cactgggtat atttcgccgg ctccaccgct
     4261 tccaccacga taggcatgaa gccgtgttcc ttgccgcaca gctcggcgca ctgaccgcga
     4321 taggtgccgg gactgtcagc cgtgaaccag gtgtcacgca catatcccgg tatcgcgtcc
     4381 tgcttggctc ccagcgcagg tacccaccag gcgtgaatca cgtcgttggc cgtaagcagt
     4441 atgcgaacct tcttgccgac ggggaccacc aggcgattgt cgacttccag caggtagttc
     4501 tcccccttgg gctgggagtt atcgatctga cccctgggcg tggccagtgc gctcataaag
     4561 ctgatccctt ccccttcgcc cgtgatgtag tcataccccc acttccattg gtagccagtg
     4621 gtcttgatcg tgatgtccgg gttggaggta tccttcattg cgatgatggt cttggtcgca
     4681 gggtaggcca tccccatcag tatcacaaag ggaatgatgg tccagatgat ctcgaccgtg
     4741 gtgctgtgat ggaagtttgc cgctttatag ccctgagact tgcgatgctt gagcattgaa
     4801 taaaacatgg tcccaaacac gcctatgaat atcaccaggc atatccatag cagcatcgtg
     4861 tgctggtcat atatgtcccg ggcaatcacc gatttcggct cgggaaaatt taactggtaa
     4921 ggatctgccg ctgccagcgc cagccctgca tacaggcccg ccgccaagcc ccacattaca
     4981 ccccacgcta cggttgatgc tttactgctc atgtctcccc ccacttgatt actgattaca
     5041 gtacgaccaa ttgtttacga actattcgcc tgatcatctc caggttattt ccttcccggc
     5101 tggatggcat tggctccgtc ctctgttaga ttcggataaa aacagggatg aggcgcaacc
     5161 ctatcccgcc cgaaacacct caccgcaaat cccacaaaaa accctattga taatgtcagt
     5221 aattatgcta ttcttatgaa acgcagacgg aatccaaaca taagaataac agcattccca
     5281 taagttcaag gttaccacca aaaaatttcg agcctgatca gatctcaaat gatcaggaac
     5341 gaaattctgc gcccctccac ctgcgtttct atccgaattt cggggccggc agcctttaaa
     5401 tcaagaaaat cgataaaatc ccttcatcat attcctggta tgaaatctgc ttatcttgtc
     5461 actcagattt catggattcg cctgtaacgc ttttgctttc ttcaggtctg gcgcggctct
     5521 cgggtgagca ttaaattcac tttccatacc cggctagatt gtcttttgcc gggccttaaa
     5581 gcattcttct atgcatttca gctatttatg gtagctgacg gccctgaaac ggagaaaaat
     5641 tctaccacag caaaattgcc cgtacaagga agaaacgaac atctttctaa tatatttgaa
     5701 tatgacccta ttctaaaaag agttatatct ccctcctgaa attggggtaa ttttacccta
     5761 atagaattgt ggtaattagg gttgggagtg gaaggcagag catatcctca tcctaccttt
     5821 cgcgatcctc ggaagacgtt ttgagcatca ttttctcacc cgcgttgatc gcttcctgcg
     5881 agtctgcccg ctccgctcat atcatcttgc tcaagcaaac gaggagccgg tcaagcccca
     5941 agcgtattcg gcaggttcag gaatatatcg acctgtccag gcaggcaaaa tagactgtcg
     6001 catccacgtc cgtcttgttc cgttgcgcct gccagatcat ttcagccagg cattccatca
     6061 cctggtgcat cgcagcatgc ttttcaccag ttttgctcaa tagcctttca aagcgatcac
     6121 gaatgccgga gggctggtct atcgagagtt gctcgtcaat ggctacgtgc atgctcatat
     6181 gcaaaaacgg attgatatcg cccatttccg gcaaataatc cttatcctga tatcgttcag
     6241 ggtcctccag cacggtctga tactccgggt gcatcaaaat cacatcgagc accatgtttt
     6301 ccatggctga caaaacctcc cgctcgcgat atttgcgcca agtgtcgaag aagaattgac
     6361 gtgcctgttc ccttgatgga ttaaacatag aacgaagatc cggaataaaa ggcttgccgg
     6421 ttagaaagtg agaggcttgg aactcaggtc ttaaagcgtc tgggtctttt ccccatattc
     6481 acacaggtcg tttataaggc agaccgggca tttaggcttc cttgccacgc atacatagcg
     6541 accgtgcagg ataagccaat gatgcgcatc atgctgaaat tgcggaggaa cgcacttcag
     6601 gagcttccgc tccacctcaa gcactgtctt tccgcgcgcg atccccgtac ggttgcatac
     6661 cctgaatatg tgagtatcca cggcgatggc gatctcccca aatgcagtat tcagcaccac
     6721 gctcgcagtc ttgcggccaa ccccgggcag ccgttccagt tcctcccgcg tccgcggaac
     6781 ctccccgccg tgatcctgga tgagtagagc gcaggtcgcc agaatattct ttgctttggt
     6841 cttgtacaat ccaatactct tgatgaactc ctgcagcccg ctttccccca gcgccaatat
     6901 tttctcagga gtattggcct tggagaacag cttcctcgtg gcaatgttca cgcccttgtc
     6961 agttgcctgt gccgagagaa tcaccgcaac gagcagttcg aagggagagc tgtactccaa
     7021 ctccgtagcg ggatgaggat tggcggcttt gaaccgctcg aagatctcgg taattttttg
     7081 agcattcatg ggataaacag gcgtttcaag acgtccttct gtgtccttct taagtttcct
     7141 tattcggttc tatcttccgc acccaactgc tgggctcgtg cagccatggc ccgctttagc
     7201 gcagcctgaa tcatggccct ttttcgatcc tggctttcct tcgctgatct ttccttcacg
     7261 ggcgtttcct tcaggtcaag ctcgaatttt tgtgattcaa tctccaggaa acgtaaactt
     7321 tcctctgcct tttccttttg gtggtcctcc ttcgcttctt ctttttctct tcgatcgcga
     7381 tcgagtctct ttaatctgaa ctggtagcgc gcacgtgc
//
LOCUS       DASSHJ010000006         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_320347, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000006 DASSHJ010000000
VERSION     DASSHJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320347"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VFI43_00320"
     CDS             complement(<1..1071)
                     /locus_tag="VFI43_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7060601.1"
                     /translation="MRIRLFTNIYFAFIILIFSLLIMLASGALSTLEDITEAERQKYR
                     SLQLASELFHSSEDLTKMARSYVVTGKPIYEHFFFEILDIRNGKRPRPEGHLINYWDM
                     DGPLTPISGGAAIPLVELMRREGFSKHELALLQQSQKNSDDLVKLERQAFAAIKGLCG
                     DHYGNLGAPCPSGRDFAINLLFGERYKAEKARIMAPISQFMLELDNRTQATLSRLQSR
                     FHQQILLMLAVLCIALLIAAAAAFYMRRNILHPLDYLRRQASSIAQGSYSTRCDISTR
                     NELAELGADFNTMANAIEREITKLKQVEGSLRERLKEINCFYAIRRGMESSSLEEVCE
                     IIFAQLIAAMQFPHLASGKIELD"
     gene            complement(1517..1810)
                     /gene="ftsB"
                     /locus_tag="VFI43_00325"
     CDS             complement(1517..1810)
                     /gene="ftsB"
                     /locus_tag="VFI43_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HET7060602.1"
                     /translation="MKILGFLLVILIIAMQYPLWLGKGGWLKVWEMDRQIAAQEETNR
                     KLQARNATLDAEVRDLKQGVEAIEERARSELGMIKENETFFHVLEDNVASSRR"
     gene            complement(1939..3225)
                     /gene="eno"
                     /locus_tag="VFI43_00330"
     CDS             complement(1939..3225)
                     /gene="eno"
                     /locus_tag="VFI43_00330"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7060603.1"
                     /translation="MSAIVDVIAREILDSRGNPTVEADVLLESGVLGRAAVPSGASKG
                     TKEAVELRDGDMQRYSGKGVLKAVENVNTEISEAIMGLDAMEQTFIDRTLIDLDGTPN
                     KSRLGANAVLAVSLAVAKAAAEESGLPLYRYLGGAGPMSMPVPMMNVINGGVHADNNM
                     DMQEFVIIPVGAQSFREALRWGAEIFHSLKDLLVAKGMYTAVGDEGGFAPNLANNEAA
                     LQLIMEAIEKAGYLAGPDIAIGIDCASSEFFKDGKYHLASDGLTLDSAQFIDYLAAWI
                     DKYPILSIEDGMSEHDWDGWKMLTQRFGKTVQLVGDDIFVTNTAILKEGIAQGIANSI
                     LIKLNQIGTLTEALSAIDTAKRAGYTAVVSHRSGETEDTTIADIAVATNVLQIKTGSL
                     SRSDRLAKYNQLLRIEEDLGDSASYPGRDAFYQLRY"
     gene            complement(3296..4267)
                     /locus_tag="VFI43_00335"
     CDS             complement(3296..4267)
                     /locus_tag="VFI43_00335"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HET7060604.1"
                     /translation="MKYWDDWRMKGYRMFFRGEAACLDTSCGKPFPLLPSLLRTRIYD
                     RLNEHWGTKGDVRKLERQILDHYIRHTAHLIETEGQFGGRTLADLEILCLAQHYGLPT
                     RLLDWTLNPFIALYFALTGSWRGGDFSKGEPSRLWVMILKKEDQRRDRAIHLERKNQE
                     DDRINRVIHLDIISNNRKIPWRDKRWAESAPFIVVPLVFTQRIAAQSGRFIYCSNLTQ
                     SEDRLDKVVTKHVFNPKYSGREGRDELVGLTEGRYYFNSKKGYHEIDEASLENKTNPW
                     AHLICYPIVQGDNKETLLKQLEFRGYHSGRLFPDLGGWATYLRQGFR"
BASE COUNT         1172 a         1250 c         1145 g         1216 t
ORIGIN      
        1 gtccaattct atttttccgc tggcaagatg cggaaactgc atggccgcaa tcagttgcgc
       61 gaaaatgatt tcgcaaacct cttccagcga actcgactcc atccctctgc ggatcgcata
      121 gaaacagttg atttccttca gacgctcgcg taatgaaccc tccacctgct tgagtttggt
      181 gatttcgcgt tcgatggcat tcgccatggt attgaagtct gctccgagct cagcgagctc
      241 gttgcgagtg gaaatatcac agcgtgtcga atagctgcct tgagcgatac tgcttgcctg
      301 gcgccgcaga taatcgagcg ggtgcaggat gttcctacgc atgtagaaag cagcagccgc
      361 agcgatgagc agggcgatgc agagcaccgc cagcatcagc agaatttgct ggtggaaccg
      421 cgactgcaat cggctcaaag ttgcctgtgt gcggttatcg agttcgagca tgaattgact
      481 gatgggggcc atgatcctgg ctttctccgc tttatacctc tccccgaata acaggttgat
      541 ggcaaaatcg cggcccgatg ggcaaggcgc gcccaaattc ccgtagtgat ccccgcataa
      601 accttttatt gctgcaaatg cctgcctttc aagcttgacg agatcgtcgg aattcttctg
      661 ggactgttgc aggagcgcga gttcgtgttt actgaatccc tcgcggcgca tcaactctac
      721 gagtggaatc gcggcccccc cagaaatcgg ggtgagcggc ccgtccatat cccaataatt
      781 gatcaggtgt ccctcgggac gcgggcgctt gccgttccgg atgtcaagaa tctcgaaaaa
      841 gaaatgctca tagatcggct tgcccgttac cacgtaactg cgagccatct tggtgagatc
      901 ttcggaactg tgaaacaact cgcttgcaag ctgcagtgag cggtattttt ggcgctctgc
      961 ctcggtaata tcttccaatg tacttaacgc gcctgaagca agcatgatca gaagactgaa
     1021 tatcagaatg atgaatgcga aataaatatt ggtaaaaaga cgtattctca tgttctcaac
     1081 cggtattctc aacgtaatgc cgcaagggct gcgcgtcgca gacgcagacg cagacgcaga
     1141 acaaggatag cctcctgggt gaactcggaa aacaggtgta cgtggtcatt ctgacaacga
     1201 tccccctcga ctctgcccat gcggcttgcg gcgtttgctc cacttcgaac tgctcactat
     1261 atacgctacg tatagccgct gcttagcagc agaataccaa agtcgatagg agtattgcgt
     1321 ttatgtctat tggggaatgg gcgacccggg aaatgagccg catgtatcac gacttatggc
     1381 ggttaagcga tacgcgggat aggaatgaag ggtcagaatc ggggcgggag cggaacgggt
     1441 tagggagcgg aacggattat agtgcgaagc gcgatgggat gctaaaccgc tgcccatgaa
     1501 aacagaccgt gcagcgctaa cggcgcgagc tcgcaacgtt gtcttccagg acatgaaaga
     1561 aagtctcatt ctcttttatc atcccgagct cactacgggc gcgctcttcg attgcttcca
     1621 cgccctgctt gagatcacgc acttcagcat ccagggtggc gttgcgcgcc tgcagctttc
     1681 tgttcgtctc ttcctgggcc gcaatctgcc ggtccatttc ccagaccttg agccaccctc
     1741 ccttgcccag ccataaggga tattgcattg caataatcag tatgacaagc agaaaaccca
     1801 gtattttcac gtgataatgc cgggaaatcc cagtggatca agtgcttcta cggcagcctt
     1861 gcaggccctt ggagggctct gaattgcgcc agctaaacgc ccgcggtcaa ctacgttgcc
     1921 ggatggaaac cagcttttct agtatcttag ctgatagaaa gcatcccttc ccgggtagct
     1981 cgccgaatcc cccaggtctt cctcgatgcg cagcaactga ttgtatttgg ccagccggtc
     2041 agatcgggaa agcgaccccg tcttgatctg caggacattg gtagcgacgg caatatctgc
     2101 gatggtcgtg tcctcggttt ctcccgagcg atgcgagaca accgccgtat agcccgcgcg
     2161 cttcgcggta tcgatagcag aaagggcctc ggtaagcgtg ccgatctggt tgagtttgat
     2221 aagtatcgaa ttggcgatgc cctgcgcaat gccttccttc aaaatggctg tattggtaac
     2281 gaatatgtca tcccccacga gttgcacagt ttttccaaac ctctgggtca gcatcttcca
     2341 gccgtcccag tcatgctcac tcatgccatc ctcgatgctc agtatcggat acttgtcaat
     2401 ccacgctgcg agataatcga tgaactgtgc cgaatccaga gtaaggccgt cggatgcaag
     2461 atggtatttg ccgtctttga aaaattccga actcgcgcaa tcgatgccta tggcgatatc
     2521 cgggcctgcc aaataccctg ctttttcaat tgcctccatg atcaattgaa gggctgcttc
     2581 gttgtttgcc agattagggg caaacccccc ctcgtcccct actgcggtat acattccctt
     2641 ggcgacaaga agatctttca atgaatgaaa tatttccgca ccccaacgga gagcttcccg
     2701 aaagctttgc gcacccaccg ggatgatcac gaactcctgc atatccatat tgttatccgc
     2761 atgtaccccg ccgttgatga cattcatcat cggcactggc atcgacatcg gccccgcacc
     2821 acccagatag cggtacaagg gcaaccccga ctcctccgcc gctgctttgg caaccgcgag
     2881 cgacacggcg agcactgcgt tggcgcccag cctggacttg ttcggagtgc catcgaggtc
     2941 aatcagcgtg cgatcgataa aggtttgctc catcgcatcc agccccatga tcgcttcgga
     3001 aatctcggtg ttgacgttct ccacggcctt gagtacccct ttgccggaat accgttgcat
     3061 atcgccgtca cgtaattcaa ccgcctcctt cgtgcccttc gacgcgccgg aaggcacagc
     3121 tgcccgtccc agcactccgg attccagcaa tacatccgct tccacggtag gattcccgcg
     3181 agaatccaga atttcgcggg caatgacatc tacaattgca ctcatgcttt tttccctgag
     3241 taatagttgg ttttattgaa aataattttt attattggaa ggtgataaga cagagtcatc
     3301 gaaaaccctg cctgagatag gtggcccatc cgcccagatc tggaaacaga cgaccggagt
     3361 ggtagcctct gaattccaat tgcttcagga gggtttcctt attatcgccc tggacaattg
     3421 gatagcatat gagatgagcc cagggattcg ttttattttc cagactggct tcgtcgatct
     3481 cgtgatatcc cttcttactg ttgaaataat accgtccctc tgtcaatccg actaattcat
     3541 cgcgaccttc ccggcctgag tatttagggt taaaaacatg ctttgtaacg actttatcca
     3601 accggtcctc actttgcgta agattcgagc aataaataaa acgtcctgac tgagcagcga
     3661 ttctctgtgt gaaaacaagt ggcaccacga taaaaggagc agattcagcc catcgtttgt
     3721 cccgccaagg aatcttccta ttattgctga tgatatcaag atggataact ctattaattc
     3781 gatcatcctc ctgattcttt ctctctaaat gaattgctct atctctcctt tgatcttcct
     3841 tcttcaatat cattacccat agccttgatg gctctccctt actgaaatcc cctccccgcc
     3901 aagacccggt taaagcaaaa tagagagcaa taaaaggatt tagagtccaa tccaatagcc
     3961 tggtgggcaa tccataatgc tgagcaaggc atagtatctc aagatccgct aatgttcttc
     4021 ctccaaattg accctctgtt tctatcaggt gagcagtgtg ccgaatatag tgatcaagta
     4081 tttgacgctc cagttttctc acatcacctt ttgttcccca atgctcgttc agacggtcat
     4141 atattctggt tctcaataat gatggtaaca atggaaaagg tttaccgcag ctagtatcca
     4201 agcatgctgc ttcacctcta aagaacatcc tgtagccctt catacgccaa tcatcccagt
     4261 acttcataac atcgaaccat gtttcggaag ttatgacggg cgagagatgt gcctgatctg
     4321 tccaccaatt acgatcaatt tgatttaggc tagacatgag gagaaaacaa ttaaaaatta
     4381 ttccgttagc atgttttata caaaggagag tacgaagtac tatataactg cgagcgtgac
     4441 tttagtcagc ggatgaacgg cgggatatgg tttttatgat tatcggcttc ccgagtttat
     4501 ttatgacctt attccaatac atggccgatg ttaaaaattc atgcttctga taggagggat
     4561 gagttaaaga agggataggt aaaaaatatg acattaacga catgttattg gaatgaaagt
     4621 agcttcttga atcaggtaat ttattacgcg atatttttta atttagcaga gtccgaacca
     4681 gccgaagcac gcttgtttga aaagtccaca gccgcagcta tgaaagtagt aaacaggggg
     4741 tgccccttct tcggcctgga agtgaattcg gggtgaaact ggc
//
LOCUS       DASSHJ010000007         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_652231, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000007 DASSHJ010000000
VERSION     DASSHJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652231"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(665..1207)
                     /locus_tag="VFI43_00340"
     CDS             complement(665..1207)
                     /locus_tag="VFI43_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HET7060605.1"
                     /translation="MKLSDGEKLILFMLGEIYEKLGIDGDIDPRFVAKAIRTGHLWAI
                     KRKYYGIYDGLTNNDADIVRDVVRLLVMWDVMEAAYDRLTEAEKDELKRQTGLSAVTF
                     GGFDANTEEQYYSVLMFLIYEMDEFSTFKGRELNAGHPMLESYMRMLAVFRTLGRMLS
                     GRSFNALELIQILNARRASD"
     gene            1648..1917
                     /locus_tag="VFI43_00345"
     CDS             1648..1917
                     /locus_tag="VFI43_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060606.1"
                     /translation="MIDSNLLITAAAVVLGLVWLGAWLLGKIRRGEIEIGTHTILLVL
                     LAAILLVPFAVNVAGHLIANSLYRPSGENGAQHEEAAPAHKHDSP"
     gene            2115..>2863
                     /locus_tag="VFI43_00350"
     CDS             2115..>2863
                     /locus_tag="VFI43_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7060607.1"
                     /translation="MLRSIRNIAIIAHVDHGKTTLVDKLLHQAGSFAAHQHIAERVMD
                     SNDIERERGITILAKNCAVDYEGVHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTKKALALGLRPIVVVNKIDRPGARPDWVVNHTFDLFDKLGANEDQLDF
                     PVVYASALNGYATLDLKQTGRDMRPLFDAILSHVPAPAGDSDGPLQLQISALDYSSFV
                     GRIGIGRINRGRLRPGQEVMVMAGASTPKKA"
BASE COUNT          651 a          752 c          742 g          718 t
ORIGIN      
        1 tccctcgttt gcccattctc tgcatgagaa acgtacgcag gaaaagctgc tgctctatca
       61 gcaattgcca cgctatataa aaaaatgtag ccagtccggc caggggaacg gccagggcat
      121 ttctcacgta ggccagcaca gttccttttt ttatttccgt cgcaatctcc atctaatgct
      181 cctttggttt ccttctcaac attcgatttc atcctggaag caatacacgc agaaccttgt
      241 cagctattgg tcaatactct ggcctctgga tgatgtgatg gataatacaa ctaactccgc
      301 cgttcaacgg caatcccata tggaggacct gggctcgtcg ggatagtcgt aaaccccttt
      361 tcctgtcgct ggactggctt taatccaccg tgggcaaaca agcagagcta ttgataagta
      421 taggaaatgt gggccccttt ttaaacgaaa aggagctcaa gcgcaacttt tatcttaacc
      481 cggcagctac ttcttgtgag gaatatgcgg ccagcaacga aatagacaag attcggttgt
      541 agttgggaca acaaccgttt ggcgaggggt gggaaaacag atggtgttag ctggtcacag
      601 caatcgtgcg tttatcgcgc agtctggccg tattttttga tttgaagatg ggcttctcac
      661 tcccttaatc gctcgcccgg cgggcgttaa gaatctggat taattccagt gcattgaagc
      721 ttctgccgga aagcatccgt cccagtgtcc tgaaaaccgc cagcattcgc atataactct
      781 ccagcatcgg gtgacctgcg ttcaactcgc gccctttaaa tgtggaaaac tcatccattt
      841 catatataag aaacatgagt acgctgtagt attgttcttc tgtattcgca tcaaatccgc
      901 cgaacgtcac cgcgctcagt ccggtctggc gttttaactc atccttctca gcctcggtca
      961 accggtcgta ggcagcttcc atcacgtccc acatgacgag gaggcggaca acgtccctca
     1021 ctatatccgc gtcgttattt gtcagcccat cataaatccc gtaatacttt cgtttgatcg
     1081 cccagagatg ccccgtacga attgcttttg ccacaaacct cggatcgatg tcaccatcga
     1141 tcccgagttt ttcataaatt tcgcctagca taaaaagaat aagtttttcg ccgtcactta
     1201 gcttcatctt cgttccattt atcaattcgc acagctggtt atcttatgcg ggcatgtgca
     1261 aataaccatt gtcctgaagt acaggaataa cggtttttct agcgaaatga tgaggagaat
     1321 gatgacaaaa acgacagccg aaaccgcgag ggttgctgag gagacaggga atacgctgtt
     1381 tttttcggct ggacttctct cgctgttcct gctgccacct gtattccatg gcgaggagca
     1441 ctgttataaa ctgtttgagt ccaggggaaa tgctaggtgt ggtttctcac aacgttgttc
     1501 ccgcagcgga cagaacttcc ccgcgctgcc cagatgggct ttgataatgg ggatatgtcc
     1561 gatgtcgctg ggcggcttca ggtgcttata agtccatagc gcatgacccg ataaagaagg
     1621 cctcgggagg tatcgggcag ggggaaaatg atagacagca accttctcat tacagccgcc
     1681 gcagttgtgc tcggtctggt atggctgggc gcctggcttt taggcaagat caggcgggga
     1741 gagatcgaga ttggcactca caccatattg ctggtgcttc tcgcggcgat tctcctcgta
     1801 ccttttgcag tgaatgtggc cggccatctg atcgccaatt ccttgtaccg cccgagtgga
     1861 gaaaatggcg cgcagcacga agaggcggct cctgctcaca agcatgattc cccatgagaa
     1921 tgatttttcc gttcgcagca tagttacagc agttcaccag gtcccggttc cagtcatgtc
     1981 aacagtctgt tttgaattgg ccattaaact tgtgcccgag tccggtgtct tagttttcct
     2041 gatataattt cacgttttgc ctcgcgccgg gcacttccga ccctggctta tcgagactcc
     2101 taccgagact acttatgctt cgctccattc gtaacattgc catcattgcc cacgtcgacc
     2161 acggcaagac aacgctcgtc gacaagctgc tgcatcaagc cggctccttt gcagcgcatc
     2221 agcatattgc cgagcgcgtg atggattcga acgatatcga gcgcgagcgc ggtattacca
     2281 tactggccaa gaactgcgcg gtcgactacg agggtgtgca catcaatatc gtcgatacgc
     2341 cgggtcacgc cgatttcggc ggggaggtgg agcgggtgct gtccatggtc gatggcgtgc
     2401 tgctacttgt cgatgcagtg gaaggcccca tgccccaaac ccgcttcgtg accaagaaag
     2461 cgctggcgct gggattgcgt ccgatcgtcg tcgttaacaa aatcgaccgt cccggggcac
     2521 ggcccgactg ggtggtgaat catacgttcg atctgttcga caagttgggt gcgaacgagg
     2581 accagctgga ttttccagta gtgtatgcct ctgccctcaa tggatacgca acgcttgatc
     2641 tcaagcagac ggggagggat atgcgccctc tgtttgacgc catactcagc catgtgccgg
     2701 cacccgccgg agatagcgat ggcccgctgc aactacagat cagcgcgctg gattattcga
     2761 gttttgtcgg acggatcggg atagggcgta tcaaccgggg gcggctcagg cctggtcagg
     2821 aagtgatggt aatggcggga gcaagcactc cgaagaaggc cag
//
LOCUS       DASSHJ010000008        16864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1193658,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000008 DASSHJ010000000
VERSION     DASSHJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 16864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16864
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193658"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFI43_00355"
     CDS             <1..253
                     /locus_tag="VFI43_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET7060608.1"
                     /translation="ESSGRIAGELRNACEAGDAQAAGAAAHKLKSSARSVGALELGEL
                     SARMEQTGKAGDIKGLKALLPQFDAQLAAVDAYIDAQQG"
     gene            296..1540
                     /locus_tag="VFI43_00360"
     CDS             296..1540
                     /locus_tag="VFI43_00360"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HET7060609.1"
                     /translation="MPEKSEVRILVLDDEPFMLKLLGHMLSNLGYTSVTLCENGRNAL
                     AQIAETGPNLILLDLNMPEMDGIEFVRHLVDRHYTGSLVLISGEDERMLQTAEKLVQA
                     HKISILGHLHKPVKPDMLSALLQKWAPPLISSSRSEKKKYSADELRTAIKEGQLINYY
                     QPKVAIATGEVVGVETLVRWRHPTDGLVFPDQFISVAEAGGLIDDLTKAVFAGAMAQA
                     RTWEDAGLPLRIAVNVSMDNLASLTFLSFVAELTAKEGIAPQHVVLEVTESRLMQDTR
                     APLEILTRLRLKRFRLSIDDFGTGHSSLAQLRDIPFDELKIDQGFVHGAWRDETLRAM
                     YEASLSLARQLGMEVVAEGVADRNDWDFLYRTGCDLAQGMFVSRPMRPEDMPGWIQKW
                     RERVRTELSVSKNGGEAEKVEE"
     gene            complement(1748..2116)
                     /locus_tag="VFI43_00365"
     CDS             complement(1748..2116)
                     /locus_tag="VFI43_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HET7060610.1"
                     /translation="MKLHLSTNTGQNLFTGYGAGYVLVNHVRYEHDVIVTPDRIIENW
                     GAEGFDQLTPEDFRFLLSLHPELVLFGSGPILRFPHPRLTRALLESGIGIEVMDTSAA
                     CRTYNILMAEGRRVVAALLI"
     gene            2378..3622
                     /locus_tag="VFI43_00370"
     CDS             2378..3622
                     /locus_tag="VFI43_00370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7060611.1"
                     /translation="MQSILKSSKLANVCYDIRGPVLDRAREMEEEGHHIIKLNIGNPA
                     VFGFDAPEEILQDVIRNLSAASGYCDSKGLFAARKAIMHYTQEKQIRDVRLDDIYIGN
                     GVSELIVMAMQALLNTSDEVLVPSPDYPLWTAAVVLAGGTARHYMCDEQSDWLPDLDD
                     MRSKINSNTRAIVVINPNNPTGALYPDDLLREIIEIARQHQLIIYADEIYDKVLYDGA
                     THTSIASLADDVLFITLNGLSKNYRAAGFRSGWAVVSGTKQYARDYISGLTMLASMRL
                     CANVPSQFGIQTALGGYQSIKDLVMPTGRLMRQRDLAWKLLTDIPGVTCYKPKAAMYL
                     FPRLDPGMYPVEDDEQLVLDLLMEEKVLLVQGSGFNWAQPDHFRVVFLPNSDDLTEAI
                     GRIARFLGRYHKRSGTGSLSSA"
     gene            3701..5017
                     /locus_tag="VFI43_00375"
     CDS             3701..5017
                     /locus_tag="VFI43_00375"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7060612.1"
                     /translation="MKPINVGLLGIGTVGGGTFAVLNRNQEEIARRAGRGITIRTVAD
                     REQEKARKIVGENVRVTGDAGEVVADPEIDIVVELIGGYTVARELILKAIENGKHVVT
                     ANKALLASHGTEIFAAAQKKGVIVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TSNYILSEMRDKGLTFDTALKQAQKLGYAEADPTFDIEGVDAAHKLTIMAAIAFGIPM
                     QFDKVYTEGITRLTREDIRYAEELGYRIKLLGIAKRTPKGIELRVHPTLIPTRRLIAN
                     VEGVMNAIVVKGDAVGATLYYGAGAGAEPTASSVVADLVDVTRMHTADPKHRVPHLAF
                     QPDLLSDVPVLPMDDVETSYYLRLRVLDKAGALADITRVLADLQISIDAMVQKEPGEG
                     EDQVDIIMLTHEAIEKNVNAAIAKIKKLPFLTGKVTRIRLEHLGGK"
     gene            5018..6445
                     /gene="thrC"
                     /locus_tag="VFI43_00380"
     CDS             5018..6445
                     /gene="thrC"
                     /locus_tag="VFI43_00380"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7060613.1"
                     /translation="MRYISTRGGMAPKRFSKILLGGLASDGGLAVPETYPQFGAAELE
                     KLRGLSYPELAFEILSRFADDIPHVDLRAIIGRTYTAEKFHSEEITPLGTLEPDLHIL
                     GLSNGPTLAFKDIALQLLGNLFEYALAKSGDHLNILGATSGDTGPSAEYAMRGKERIR
                     VFMLSPQGKMSPFQTAQMFSLQDPNIFNIAIRGVFDDCQNIVKAVSNDSTFKQKYRIG
                     TVNSINWARIAAQIVYYFKGYFAATRSNNEPVSFAVPSGNFGNICAGHVARMMGLPIR
                     RLILATNENNVLDEFFRTGRYRPRSTEETRHTSSPSMDISKASNFERFIFDLTGHDAD
                     KVRELWRAVDEGGEFDLAGTPLWEKVEDFGLVSGSSSHADRIETIRKVRENYGLLVDP
                     HTADGLKVGMEYREAGVPLICLETALPVKFSKSIIEAIGEEPPRPPGYANIESLAQRY
                     VVMNPDADAVKGLIAERSDRTAALA"
     gene            complement(6570..7346)
                     /locus_tag="VFI43_00385"
     CDS             complement(6570..7346)
                     /locus_tag="VFI43_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380881.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7060614.1"
                     /translation="MTRPLRIGLSPRILHHPPPELGFRNKTLQYLEQTVAHWIIRRGA
                     LAFMLPALETGGVERSKVRISDYVREIDGLVLQGGADVSPVSYGEEPARPEWSGDRVR
                     DLYEMELFWECVVQGKPVLGICRGLQLINVALGGSLYQDIVTENPLAIPHVDPVLYDQ
                     HRHSLLIEEGSRLAKLYDDDQPHLVNSIHHQAIKRLGRDSEVEAVSESDGIIEAIRMR
                     GDCYVTGFQWHPEFHGGAKELLDSGPILDDFLTAAERQRA"
     gene            complement(7538..8653)
                     /locus_tag="VFI43_00390"
     CDS             complement(7538..8653)
                     /locus_tag="VFI43_00390"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET7060615.1"
                     /translation="MSLMPFASSRPLSMGVELELQLLSCNDYNLVSSAPDILRRVSKR
                     AHPGEIKPEMTRSMIEINTSVQHEYSGLVNELHALRDVVSDAGRFLNIAVAGGGTHPF
                     QHWSEQKIFDAPRFHYLSELYGYLAKQFTIFGQHVHVGCPGPDEALHLMHMLSRYIPH
                     FIALSASSPFVQGHDTGFASARLNSVFSFPLSGRAPFVLRWEDFEKFFSKMTSTGVVE
                     SMKDFYWDIRPKPEFGTIEVRVCDTPLTVEIAAAIACYIQAMSRYIMVEQRIAPEEDD
                     YLVYTFNRFQACRFGLEGVFIDPRTHEQRSIREDITDMLSRIADHARELRATEAMERI
                     REILINGNGTTWLRKTYSREHNLGDVMQLQAELWMRA"
     gene            complement(8658..9869)
                     /locus_tag="VFI43_00395"
     CDS             complement(8658..9869)
                     /locus_tag="VFI43_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7060616.1"
                     /translation="MTWSDYLPIWPLAITPALWIALTLVVAVLVGEALVRHLRLPRIV
                     GYVGVGLLLGPHAFGLIPELPLTEWRLVIDLALGILLFELGSKVNLHWLKTNRWIAGA
                     SLLEAAATFFLVFALLVWMDTPGATAAVVAAILIATSPAIVVRTITESRAQGQVTDRL
                     LLMTALNCIYAVILHKLTVAAMHGAAGTSALHTILQPLYLIGGSALLAWLFGFTFERI
                     HHYLGHHEETFSFILFGMIAFATIMASTLKLSPVLVLLAAGLITRYHRQRPRTFPPHF
                     GSAGAVLVVLMFIGNGLAGNLSGLRESFLLVWLLIGVRAVAKISSVLALAHHSGLSLR
                     QGTALGVALMPMSSVTLLLTLETSATFPSFASGFGLTLMSCIVILELIGPILVQVSLR
                     RAGETPETSQR"
     gene            10191..10646
                     /locus_tag="VFI43_00400"
     CDS             10191..10646
                     /locus_tag="VFI43_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HET7060617.1"
                     /translation="MRLDKEGVGDPYNLRRFLDAQQPVYEAVRGELKDGCKRSHWMWF
                     VFPQIEGLGQSHMAGKYAIKSREEGNAYLDHPVLGHRLRECAALVANLDGLLIEDIFG
                     YPDDLKFRSSMTLFGEIAPGGGIFRECLEKYFEGKPDVKTLALLSARPS"
     gene            10931..11131
                     /locus_tag="VFI43_00405"
     CDS             10931..11131
                     /locus_tag="VFI43_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060618.1"
                     /translation="MKTARLLFIILFMTAGASSLAWAGEPNPDALTGGQKSEKSKAMP
                     GNDCGPEASQEVSRPDAATLAE"
     gene            11598..12116
                     /locus_tag="VFI43_00410"
     CDS             11598..12116
                     /locus_tag="VFI43_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET7060619.1"
                     /translation="MKNSPWVAGILIAATLTGCAGVPDRNAARAGYGGSILEQPGPRV
                     DLSDTALVKDILYTQFREWKGTPYQLSGLSKNGIDCSGFVHITFRSRLGVSLPRSSEL
                     QAEMGIDIDKDELRAGDLVFFKTGKKLKHVGVYLEGGRFLHASTRLGVVISGLDESYW
                     KSAYWKAKRLET"
     gene            12263..12613
                     /locus_tag="VFI43_00415"
     CDS             12263..12613
                     /locus_tag="VFI43_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060620.1"
                     /translation="MSDSNKKIPCKLLLFFSLYFILGNAAVWAEPDLEEIQRRFNQET
                     ISKPFSVPDSATLASALKEATDRGTPTKSSSQMIPNVPLLGGLGYYGAYARPYYGGYY
                     GAGYYNPYYGGWGW"
     gene            12739..13515
                     /locus_tag="VFI43_00420"
     CDS             12739..13515
                     /locus_tag="VFI43_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HET7060621.1"
                     /translation="MESKHAIALAAAMAFSAILGGCAAVHTSIAKKDLDVQTKMSDTI
                     FLDPVEPARKIVYLNVRNTSDKANFDIAGPVARVLEGKGYTITPDPKKAHYWLQVNVL
                     SVEKASPTAAQAALGAGYGGAWGAAGSAAAGAAIGTATGAAIGGWNAAGIGGLAGVAA
                     LGLADTVASAAVKDVTYMAVTDVEIAERVEAGVIVRQDNQQDAKQGIGGALRQTSTKV
                     SNMNKYRTRVVSTANKANLQYEEAATDLTSGLTRSISGLF"
     gene            13663..14004
                     /locus_tag="VFI43_00425"
     CDS             13663..14004
                     /locus_tag="VFI43_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060622.1"
                     /translation="MNKKLTSILLAAGILFASAIGHAQKDGGSADGKKGLDRMSKELG
                     LSADQRSKVEAIFEVERRKVEAIFNEERQKLQVVQQDTRTSLEGVLTPEQMNKLDQKM
                     RENAKNGTKKK"
     gene            14163..16511
                     /locus_tag="VFI43_00430"
     CDS             14163..16511
                     /locus_tag="VFI43_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HET7060623.1"
                     /translation="MLPSIEHRISLELAVRPVQVTAAIALLDDGATVPFIARYRKEAT
                     GALDDIQLRQLEERLRYLRELEDRRAVIIASIKEQGKMTPPLLALITDAEDKTQLEDL
                     YLPFKKKRRTKAQIAREAGLEALADALLANPALQPEEEATKYLKPAFATDQGDNPGVE
                     DTRAALEGARQILMERFAEDASLLQGLREYLLEHGVVETTVAKGKEDEGAKYSDYFSY
                     SEKITTIPSHRALAIFRGRREEILHVTLRLDSEPERPKWEAPHNPCEARIATHFSIKN
                     LARPADQWLSETVRWTWRVKISLHLETELMTELRRRAEAEAIEVFARNLKALLLAAPA
                     GPQVTMGLDPGLRTGVKVAVVDATGKVLETTAVYPHPPRNDWDGSLQALGKLVEKHGV
                     SLIAIGNGTASRETDKLAKELIKRRADLRLASIVVSEAGASVYSASELASRELTDMDV
                     SLRGAVSIARRLQDPLAELVKVDPKSIGVGQYQHDVTQTHLARSLDAVVEDCVNAVGV
                     DVNTASQPLLERVSGLNSSTAHSIVAYRDMHGVFPSRAALRKVPRLGEKTFEQAAGFL
                     RIQNGDNPLDASAVHPESYPVVEKMLADIKQDIKTVIGNEKLLKSLNPAKYTDGKFGL
                     PTVTDILNELEKPGRDPRPEFTTATFKEGVNEIADLRLGMMLEGVVTNVAAFGAFVDI
                     GVHQDGLVHISALAHKYVKDPHSVVKVGQVVKVKVLEVDEKRKRIGLTMRITEKVSQL
                     EKNGNETQRGRARKEALTPQQQPKQKHLTNTAMAAAFAKLKG"
BASE COUNT         4077 a         4330 c         4815 g         3642 t
ORIGIN      
        1 agaaagttcg ggcagaatag caggggaact gcgcaatgcc tgcgaggcag gagatgctca
       61 ggctgcgggg gctgcggcgc ataagctcaa gtcttcggcc cgctccgtgg gcgctttgga
      121 gttaggtgaa ttgtccgcca ggatggagca aaccggaaag gcgggcgaca tcaagggact
      181 gaaagcgttg ctgccacagt tcgacgccca attggctgct gtagatgcct atattgacgc
      241 tcagcaggga tgactggttt ccatataaaa atagaataag gagcaggcaa aggcgatgcc
      301 agaaaaatct gaagtcagga tcctggttct tgacgatgag cccttcatgc tgaagctgct
      361 gggccatatg ctatcgaatc tcgggtacac atcggtgacc ctctgcgaga atggccgaaa
      421 tgcgcttgcg caaattgccg aaaccgggcc caatcttatc ctccttgatc tcaatatgcc
      481 tgaaatggat gggatcgagt tcgtgcggca cctggtcgac cggcactata cgggaagcct
      541 ggtcctgatc agcggcgagg atgagcgcat gctgcaaacc gccgagaagt tggtgcaggc
      601 gcacaagatt tctatccttg gacacctgca taagcctgtc aagccggaca tgctgtccgc
      661 tctcctccag aagtgggcgc cgcccttgat tagttcgtca aggagtgaaa agaaaaaata
      721 cagtgctgat gaattacgca cagcgatcaa ggaaggccag ttgatcaact actaccaacc
      781 caaggtcgca atcgctactg gggaggtggt cggggtcgag actctagtgc gctggcgcca
      841 tcccacggat ggacttgtat ttcccgatca atttatcagt gtcgccgaag cgggcggtct
      901 gatcgacgat ctcacgaagg cggtatttgc cggcgcaatg gcgcaggcca ggacgtggga
      961 ggatgccggt ttgcctttgc ggatcgcggt taacgtttcg atggataacc tcgcatcttt
     1021 gactttcttg agtttcgttg ccgagttgac tgcaaaagag ggcattgccc cacaacatgt
     1081 cgtattggaa gtaaccgaaa gccgactcat gcaggatacc cgtgctccgc tggagattct
     1141 gacccggctg cgccttaagc gcttccgatt atcgatcgac gacttcggca ccggacattc
     1201 ttctctcgct caactgcgtg acattccctt tgacgaactc aagatcgacc agggatttgt
     1261 gcacggcgcg tggcgcgatg aaacgctgcg ggcgatgtat gaagccagcc tgtcgctggc
     1321 caggcaattg gggatggaag tcgtcgcgga gggcgtggcc gaccggaacg attgggattt
     1381 tctctacagg acgggctgtg atcttgcaca aggaatgttc gtctcgcggc ccatgcggcc
     1441 ggaagacatg cctggatgga tacagaaatg gcgcgagcgg gtgcgcacgg agctttccgt
     1501 ttcaaaaaat ggcggagagg ccgaaaaggt cgaggaatag agattcagga atagatgcta
     1561 acgaccagac tgaccgcggc gtgagtaagg cattccccgg taaggcactc cgcggcaggc
     1621 aatagacaca cgggaattta ataaaaccga attgcgatgt gaagatccgc tattgttcaa
     1681 gatgatttga ttgcgacaaa cggagcgtct acggtagaag gtttctgtat ctgggtagat
     1741 tgaagagtta aattagcaac gcggccacca ccctgcggcc ctcagccatc aatatgttgt
     1801 atgtccggca ggcggcactg gtgtccatca cctcgatgcc gattcccgat tcgagcagcg
     1861 ctctggtcag cctcgggtgg ggaaagcgta ggatcggccc gcttccgaac aataccagct
     1921 cggggtgaag agagagcaaa aagcggaaat cctcgggcgt gagttgatca aaaccctccg
     1981 caccccagtt ttcgataata cgatcaggtg tcacgatcac gtcatgttca taacgaacgt
     2041 gattgaccag aacgtatcct gcgccgtaac cggtgaagag gttttgcccg gtattcgtgg
     2101 aaagatgcag cttcaacttg tctccatttg cggcaggtga gccggtcagg taacattaca
     2161 acttttttga gccgcaataa agcccagcaa tggccctgga ctgagcgttc tgctagctcg
     2221 tcgatgtgtg actccggcgc catgacgtta agctgatcat tgtgaagtct cggtttcctg
     2281 ttgtccatat tcttgaatat tccttgttca cgccttttcc ggtttcgacg cctgcggccc
     2341 ggcctcgtct tcttcccgtc cccttcccaa ctcctctatg cagtccattc tgaagtcaag
     2401 caagctcgct aatgtctgtt atgacattcg cggccccgtg cttgaccgtg cgagggagat
     2461 ggaggaggaa ggccatcaca tcatcaagct caatatcggc aacccggcgg tattcgggtt
     2521 cgatgcacca gaggaaattc tgcaggatgt catccgcaat ctatcggctg cttccggcta
     2581 ctgtgactcg aagggactgt tcgcagcgcg caaggcgatt atgcattaca cgcaggaaaa
     2641 acagattcgt gacgtccggc ttgatgatat ctacatcggc aatggcgttt cggaactgat
     2701 tgtgatggcc atgcaggcgc tgctgaacac gagcgacgaa gtgctggttc cttcgccgga
     2761 ttatccgcta tggacggccg cggttgtgct ggcggggggc accgcccggc attacatgtg
     2821 cgatgagcag tccgactggc tgcctgatct ggatgatatg cgctcgaaga tcaattccaa
     2881 cacccgtgcc attgtcgtca tcaatccgaa taaccctacc ggagccctat acccggatga
     2941 cctgttgaga gaaatcatag aaatcgcgcg ccagcaccag cttatcatct atgccgacga
     3001 gatttatgac aaagtactct atgatggcgc gacacacacg tccattgcct ccctcgcgga
     3061 cgatgtgcta ttcattacgt tgaacgggtt gtcaaaaaac taccgggcgg ccggttttcg
     3121 ctcggggtgg gcggttgttt ccggaaccaa gcagtatgcg cgtgattaca tttccggttt
     3181 gaccatgctc gcctcaatgc gcctgtgtgc caatgtcccc tctcaatttg gtatccagac
     3241 ggcactgggc ggctatcaaa gcatcaagga cctggtcatg ccgacggggc gtctcatgcg
     3301 gcagcgcgac ctggcgtgga aactgcttac cgatattccc ggggtgacgt gctataaacc
     3361 gaaggccgcg atgtacctgt ttccccgcct tgaccctgga atgtatcccg tggaggatga
     3421 tgagcagctc gtactcgatc tgcttatgga agagaaggtg ttgctcgtcc agggcagtgg
     3481 cttcaactgg gcccagccag accattttcg cgtcgtcttt ttaccgaata gcgacgacct
     3541 gactgaagcc ataggacgca tcgcgcgttt ccttggccga tatcacaagc gcagcgggac
     3601 tggatcactc tcgtccgcat aatccaacct gaaagcctga tccggccact tctgctgtga
     3661 aatgaccgat ataactgaaa acttcgtgat taccgaggat atgaaaccta ttaacgtcgg
     3721 gctattgggt attggcaccg tgggcggcgg cacattcgcc gtgttgaacc gcaaccagga
     3781 agagattgcg cgtcgtgccg ggcgcggcat aacgatccgc accgttgccg atcgcgagca
     3841 ggagaaagcg cggaagatcg tgggtgagaa cgtcagggtt accggtgacg ccggcgaggt
     3901 cgtcgctgat cctgagatcg atatcgtggt tgaattgatc ggcgggtaca cggttgcaag
     3961 ggagctcata ctgaaggcca tcgagaatgg taaacacgtc gtcaccgcga acaaggcgct
     4021 gctggcgtcg cacggcaccg agatctttgc cgcggcgcag aagaaagggg tgatagtcgc
     4081 attcgaggcg gctgtagcag gtggaattcc catcatcaag gcgttgcgcg agggtttgac
     4141 tgccaaccgc atcgagtgga tcgccggcat catcaacggt accagcaact acatcctatc
     4201 tgaaatgcgc gacaagggcc tgacgttcga cacggctctg aagcaggcgc aaaaactggg
     4261 ctatgcagag gctgatccga cctttgacat cgaaggcgtc gatgcagcgc acaagcttac
     4321 catcatggcg gcgattgctt tcggaatccc catgcagttt gacaaggtct acacagaagg
     4381 gatcacaagg ctgacccgag aggatatccg ctatgctgag gaactgggtt accgcatcaa
     4441 gctgctgggt attgcaaagc gtacgccaaa gggtatcgaa ctgcgcgttc atcccacgct
     4501 gataccaaca cggcggttga ttgcgaacgt ggaaggcgta atgaatgcga tcgtcgtaaa
     4561 aggcgatgcg gtgggcgcaa cgctgtatta tggcgccggc gctggcgcag aaccgacagc
     4621 aagttcggta gtggcagatc ttgtggatgt gacgcggatg cacactgcgg acccgaagca
     4681 tagggtccct cacctggcgt ttcagcccga tcttctctct gacgtgccag tcctccccat
     4741 ggatgacgtg gagacctcct attacctgcg gttgcgagta ttggataaag cgggggcgct
     4801 ggcggatatc acgcgagtgc tggcggacct ccaaatctcg atcgatgcaa tggtgcagaa
     4861 ggaacctggc gagggcgagg atcaggtgga cattatcatg cttacccatg aagcaataga
     4921 aaagaatgtg aacgccgcga tcgcgaagat caagaagctg cccttcctga cgggaaaggt
     4981 gacgcgaatc cggctcgagc atttgggcgg gaagtagatg cgttacattt ccactcgcgg
     5041 cgggatggcg ccgaaaagat tttcaaaaat cctgctcggc ggccttgctt ccgatggcgg
     5101 acttgccgtg ccggaaactt atccacaatt tggcgctgcc gagcttgaaa agctgcgtgg
     5161 cctgagttac cccgaacttg cattcgagat tctatctcgc tttgcagacg atattcccca
     5221 cgtggatctg cgcgccatca tcgggcgcac gtacactgcg gaaaaatttc acagcgagga
     5281 gattactccg cttggaacac tcgagcccga cctgcatatt ctcgggctat ccaacggccc
     5341 gaccctggcg ttcaaggaca tcgccctgca gcttctaggc aacttgttcg agtatgcact
     5401 ggcgaaatcc ggcgatcacc tgaatatcct gggggcgact tccggcgaca cgggtccgag
     5461 tgcggaatac gcaatgcgcg gtaaagagcg cattcgcgta ttcatgcttt ctccacaagg
     5521 gaaaatgagc cctttccaga ccgcgcagat gttttccctc caggacccga acattttcaa
     5581 tatcgccata cgcggtgttt tcgacgattg ccagaatatc gtcaaggcag tgagtaatga
     5641 ttcgacattc aagcaaaaat accgcatcgg cacggtcaat tcaatcaact gggcgcgaat
     5701 cgcggctcaa attgtctatt atttcaaggg ttactttgca gcgacacgtt caaataacga
     5761 accagtgtca tttgcggtac cttcgggaaa tttcggaaac atctgcgccg ggcatgtagc
     5821 ccgcatgatg ggattgccga tccgcaggct gattcttgca accaatgaaa ataacgtcct
     5881 ggacgaattt ttcaggacgg ggcgctatcg ccctcgcagt actgaagaga cccgccacac
     5941 gagcagcccg tccatggaca tttccaaggc atccaacttc gagcgtttca tttttgacct
     6001 gacagggcat gacgcggata aagtcaggga attatggcgc gccgtggacg agggcgggga
     6061 gttcgacctg gccgggacgc cgttatggga gaaagtggag gatttcggtc tcgtctcggg
     6121 gagcagcagc catgcggatc ggatcgagac gataaggaag gtcagggaaa attacggcct
     6181 attggtggat ccccacactg ctgatgggct gaaagttgga atggaatatc gcgaagcggg
     6241 cgtgccgctc atttgcctgg aaacggcgtt gcccgtcaaa ttttcgaaga gcattatcga
     6301 ggcgataggc gaggaacctc cccgtccccc aggatatgca aacatcgaga gcttggcgca
     6361 acgctatgtg gtcatgaatc cggacgccga tgcggtcaaa gggctgattg ccgagcgcag
     6421 cgataggaca gccgcgcttg cttaacccgg gttatgtttt aagggataac gcaggagcgt
     6481 ggaggggtgg cataatgggt tgcgtttcac tccacctatt cttgtaaatt accggcgctg
     6541 gtggcatcgc agcgataaaa acgcagttcc tatgcgcgtt gcctctcggc agcggtaaga
     6601 aaatcatcca ggatcggccc ggagtcgagc agttccttgg ctccgccgtg aaactcgggg
     6661 tgccattgaa accccgtcac atagcaatcc cctcgcatgc gtatggcctc gataatgcca
     6721 tcgctttctg aaacagcctc cacctctgaa tcgcgtccca atcgcttgat tgcctggtga
     6781 tggatgctgt tgacaagatg gggttggtcg tcgtcgtata gctttgccag cctcgagcct
     6841 tcttcgatca ggaggctatg gcggtgctga tcatacaaga ccggatcgac atgcggaatg
     6901 gcaagcggat tttcggtcac gatatcctgg tagagacttc ctcccaatgc cacgtttatg
     6961 agctggaggc cgcggcagat gcccagcacg ggttttccct ggacgacgca ttcccagaat
     7021 agctccattt cgtagaggtc gcgcacacgg tcacccgacc attcaggccg agcgggttct
     7081 tcgccgtagg aaacaggact gacatccgcg cctccctgta aaaccaggcc atctatttcc
     7141 cttacataat cactgatgcg caccttcgag cgttcgacac cgccggtttc gagggcaggc
     7201 aacatgaacg ccaaagcgcc gcgccggatg atccagtgcg cgacggtttg ttcgaggtat
     7261 tgcagtgtct tgttacgaaa gcccagctct ggcggcggat gatgcagaat acgaggggac
     7321 aggcctatcc gtagcggacg agtcatgttc aatagttggt gcgatgcagt caggaacccg
     7381 taatttggat tgcgcccgcg atgggacgtt catgcacatc atcgcatctc tggcgcactt
     7441 gatctgaggg ttacttaacg gattagcctc gatctggttt attgctcttc gagacgggta
     7501 gtttggaggc aatgagaaga acaaaaggga taagggctca ggcgcgcatc cacagctcgg
     7561 cctgtagttg catgacatca ccgagattgt gttcccgcga ataggttttg cgcagccagg
     7621 ttgtgccatt gccatttatc agtatttcgc gaatcctttc catcgcctcc gttgccctca
     7681 gttcgcgagc gtggtcggct attcgtgaaa gcatatcagt aatatcttcg cgtatgctgc
     7741 gctgctcatg agttcgtggg tcgataaaga ctccttccag accgaagcgg caggcctgga
     7801 atctgttgaa ggtgtaaacc aggtaatcgt cctcttccgg tgcaatgcgt tgctccacca
     7861 tgatatagcg ggacatggcc tgtatatagc aggcgatggc ggcggcaatt tcgacagtga
     7921 gcggcgtgtc gcacaccctt acctcgatcg tgccgaattc cggcttggga cgtatgtccc
     7981 agtaaaaatc cttcatcgat tcgaccactc ccgtagaagt catcttgctg aagaatttct
     8041 cgaaatcctc ccagcgaaga acaaagggcg cccggccgct cagaggaaac gaaaaaaccg
     8101 aattgaggcg agcggaagcg aaaccggtgt catgcccttg cacgaacggg gaactcgccg
     8161 acaacgcgat aaagtgggga atatagcgag acagcatatg catgagatgc agcgcctcgt
     8221 cgggaccggg gcaccccaca tgcacgtgct gcccgaatat cgtgaactgt ttcgcaaggt
     8281 aaccgtacag ttcggacagg taatggaacc ggggagcatc aaaaatcttc tgctcgctcc
     8341 agtgctggaa gggatgcgtg cctccacccg ctacagcaat gttgagaaag cggccggcgt
     8401 cggaaaccac gtcccgtagg gcatgaagtt cattgaccag cccgctatat tcgtgctgca
     8461 cggaagtgtt gatttcgatc atgctgcgcg tcatttccgg ttttatttcc cccggatgtg
     8521 cccgcttcga cactcgcctc aggatatccg gtgcggagga aacgaggttg taatcgttgc
     8581 agctcaacaa ttgcagctca agctccaccc ccatgctcaa gggacgcgat gacgcaaaag
     8641 gcatgagact catgatttca tctctggctg gtctccggtg tttccccggc tctgcgcagg
     8701 gatacctgca caaggattgg cccgatgagc tcgaggatga caatgcagct catcaaagtc
     8761 agcccaaatc ctgacgcaaa agaaggaaag gttgcgctcg tttcgagcgt cagcaagagc
     8821 gtcacgctgg acatcggcat gagggcaacg ccaagcgcag tgccttgacg caaactcagg
     8881 ccgctatgat gagctagcgc gagcacggaa gatatcttgg caacggcccg aacgccgata
     8941 agtagccata cgagcaggaa gctttcccgc aatccgctca ggtttccggc aaggccgttg
     9001 ccaatgaaca tcagcacgac caggacagcc ccagccgacc cgaagtgtgg cgggaaagtt
     9061 cgcggacgct gccggtgata gcgcgtgatc aggcccgccg caagcagcac gagaacaggg
     9121 ctgagcttga gggtcgaggc catgatggtt gcaaacgcga tcatcccgaa aaggatgaat
     9181 gaaaaggttt cctcgtgatg gcccaggtaa tgatgaatgc gttcgaaagt gaaaccgaaa
     9241 agccaggcaa gcagtgccga accccctatg aggtagagcg gctgcaagat cgtatggagt
     9301 gcgctggtac cggcagcgcc atgcatggcg gcaacggtca gcttgtgcag gatgacagca
     9361 taaatgcagt ttaatgcagt catgagcaga aggcggtcag ttacctgtcc ctgcgcgcga
     9421 gattcggtta tcgtgcgcac gacaatcgcg ggagaggtcg caatgaggat tgcggcgacc
     9481 accgcggcgg ttgcgccggg agtatccatc cagaccaata gcgcaaacac caggaaaaac
     9541 gtcgctgccg cttcgagcag gctagcgccc gctatccagc gattagtctt gagccagtgg
     9601 aggttgactt tgctccccag ctcgaataaa agaatgccaa gcgcaagatc gatcaccaac
     9661 cgccattctg tcagcggcaa ttccgggatc agcccaaacg catggggacc gagtaacagt
     9721 cccacaccta catagccgac gatccgcggc aacctcaggt gccgcaccag cgcttcgccg
     9781 accagcaccg cgactaccag cgtcaaggcg atccagagcg caggcgtaat cgcaagcggc
     9841 cagatgggaa gataatccga ccaggtcatg ctgaaaacga tgggctatgg ccgaaaaaga
     9901 cggcgatagc aaatgtttgc gtgactttca gatgcattca ggcccctctg gtgaattggg
     9961 atcaatacga aacagtttcc gagaacgccg cgtcggaagc acgaggaact taaaaactgg
    10021 attcagaaac tgctatgtcg caatttattg acgcgatgat ttctgcgcca acttatttcc
    10081 ggaagagaca agcattataa accagaatga tgataggctt ctttcaacag cgacgggtac
    10141 gacatggggg atcgacataa atctcaactt ggcatgagtc gcggaagtaa ttgagattgg
    10201 acaaggaagg cgtgggcgat ccgtacaacc tgcgacggtt cttggacgca cagcaacccg
    10261 tttacgaagc cgtacgcggg gaacttaaag atggctgcaa gcgcagccac tggatgtggt
    10321 tcgtttttcc tcagatcgaa ggcctgggcc aaagccacat ggcaggaaaa tatgcaatca
    10381 agtcgcgtga agagggcaac gcttatctcg accaccccgt tctaggtcac agacttcgcg
    10441 agtgcgcggc cttggtcgct aacctggacg ggctcttaat cgaagacatc tttggctatc
    10501 cagacgattt gaagttcaga tcgtcgatga ctctgttcgg agagatcgcc cccggcggcg
    10561 ggatttttcg ggaatgtctg gaaaaatatt tcgaaggaaa accggacgta aaaacgcttg
    10621 cgctattgtc cgcgaggcca tcgtgaggag agaaggcatt gcgtgataat gcatgtaaaa
    10681 aatccggcct aatctgagcg cgccttcttc caaccctctt cagtactcct gtccgtccgt
    10741 aaatgactga ccgttgtcag ttcagctccg ccccaagcat ataaccacta tcagagtatg
    10801 ggattttatg gtataaataa ttatggccgc cgggatggaa ttttcgatct gtcggcagcc
    10861 tccaaggttt aaggtaggct tcacgcggaa gcaccttttt ctattacttt ttgagctcaa
    10921 ggagactctc atgaaaaccg cgcgtttgct gttcattatc ctttttatga ctgcgggtgc
    10981 ctcttctctg gcatgggctg gagagccgaa ccccgatgct ttaaccggcg ggcaaaagag
    11041 cgagaaatca aaagcaatgc ctggcaatga ttgcggcccg gaagcaagcc aggaagttag
    11101 caggcctgac gcagcaaccc tggccgaatg aatataagtt tgccaataat cgcattacaa
    11161 gtttggcatt attcgggccg ctagtaatgg ctgttcgttg tccactcgtg ttaccgggtt
    11221 ttttccagga atactcgcat gagcgcccgt ctctctttcc agacgggcgc tttctccccc
    11281 cctcgcaagc caccgttttt cttgcccttt tgactcagca gtaaagcctg caatttcgat
    11341 tgccgagatt gcatattgca tttagaattt cctgtgagcc acggttaggc ttcttcaagc
    11401 tcatccgtaa attatttcag aaatcgcata ccgaagccga catgctcgtt tgccaggtgt
    11461 atctctacgc acactaggag catgctcggc tagacccttg agctttacga acttgctgtg
    11521 cgttaagctc aaggccgaat cccagccaac gtttgagcga taacgaacaa taaaaatatt
    11581 acctggtaaa aatgtccatg aaaaacagtc cttgggtagc tggaattctc attgccgcaa
    11641 ccctgacggg gtgcgcgggc gttcccgata gaaatgcggc aagagccggt tatggcggtt
    11701 cgatcctcga acaaccgggt ccgagggttg atctgagcga cacggcttta gttaaagata
    11761 tcctttacac ccagtttcgc gaatggaagg gaacgccgta ccagttaagc gggttgagca
    11821 agaacgggat cgactgttcg ggtttcgttc acatcacgtt caggtcaaga ctgggcgtaa
    11881 gcttgccgag atcaagtgag ttgcaggcgg aaatggggat cgatatcgat aaggacgagc
    11941 tgcgcgcagg ggatctggtc tttttcaaaa ctggcaaaaa actcaagcat gttggcgtct
    12001 atctggaggg aggcagattc ctgcacgcgt caaccaggct cggcgtcgtc atttctggcc
    12061 ttgatgaaag ctactggaag tccgcatact ggaaggcgaa gcgtttggaa acctgatctt
    12121 gcgatagctt ggcaaacagt catggagcag ggaagatgtg gcggacgcac tcatctcgat
    12181 atatgccggg atgagtgatc gagttttaca actggacgag aatcaatcga cgatttcggt
    12241 tttaatgcta tgggggtgtt aaatgtccga ttcgaacaag aaaattcctt gtaagctttt
    12301 actatttttc tccctgtatt ttatcctggg caacgctgcc gtatgggcag aaccagacct
    12361 ggaggaaatc cagcgacggt ttaaccagga aacgatttcc aagcctttca gcgttcccga
    12421 cagcgccact ctggcctccg cgctaaagga agccacggac aggggtactc cgaccaagtc
    12481 atcctcccag atgatcccaa atgttcccct tcttggcggg ctgggctact acggagcata
    12541 cgcccggccg tactacggcg gctactatgg agcgggatat tacaaccctt actatggagg
    12601 ctgggggtgg taggagacaa gagcagcgtt ggacaaaaat aaagcaataa gaagcaatgt
    12661 gtattcctgg gggcaacttt ttatcacctc cacccatttt atatttaact aataacaaga
    12721 gggaacaata tgagttacat ggaaagtaag catgccattg cgctggcggc tgcgatggcg
    12781 ttttctgcaa tcttgggcgg ttgcgccgct gttcacacat cgattgcgaa aaaggatttg
    12841 gatgtccaga ccaaaatgag cgataccatt ttccttgatc ctgtcgaacc tgcacgaaag
    12901 atcgtttatc tcaacgtgcg taacacatct gacaaggcaa attttgatat cgcagggccg
    12961 gttgcgagag tgctcgaggg gaagggatac acgattactc ccgatcccaa aaaagcccat
    13021 tactggcttc aggtgaatgt gcttagcgtc gagaaggcaa gtccgacagc cgcacaggcg
    13081 gcgctgggcg cgggttatgg cggtgcttgg ggcgcggccg ggagtgcagc agcgggggct
    13141 gccataggaa ctgcgacggg cgcggcgatc ggtggatgga atgctgcagg gattgggggg
    13201 cttgcgggag ttgcggccct ggggcttgcc gataccgttg cgagcgcggc ggtgaaggac
    13261 gtaacataca tggctgttac ggatgtggag attgcggagc gggtcgaggc aggcgttatc
    13321 gttcgccagg acaatcagca ggatgccaag caggggatag ggggagcatt gcggcagacg
    13381 tccacgaagg tcagcaatat gaacaagtat cgtacacggg tcgttagcac tgccaacaag
    13441 gcaaatcttc aatatgagga agcggccacc gatttaacga gcggattgac acgttctatt
    13501 tctggccttt tctagcgcct ctgcaggaac ggcccttgaa ccacgccgtt cctgctttct
    13561 tgcctcgtga ccgcccggct gtttcggaaa tctacccggt tttatccatc ccatcccggt
    13621 tcgtgcctaa gtgcagtgca atcaagttcg agggaattaa taatgaacaa aaaactgacg
    13681 agtattttat tagcggcagg tattcttttt gcctctgcga tcgggcatgc ccaaaaagat
    13741 gggggatcgg cagatggcaa gaagggcctc gatcgcatga gcaaggaact gggtctcagc
    13801 gcagatcaga ggtccaaagt tgaggccatc tttgaggtag agagacgcaa agtcgaagca
    13861 atcttcaatg aagaaaggca aaaattgcaa gtggtgcaac aggatacacg cacaagcctg
    13921 gaaggtgtgc ttacccccga gcagatgaac aagctggatc aaaagatgcg tgaaaacgcc
    13981 aaaaacggga cgaagaaaaa ataaaggaat aagaaaaaag gaagaaatgg aagaagtgga
    14041 cggagcacgc cttcttgcct tctgttttat gagagtaggc aactgggaaa tcaaatagga
    14101 taatcgaggg tttttgcacg acagaaatcc gtgcaataca ctatttatcc aattttcccc
    14161 atatgctccc atccatcgaa caccgtattt ccctcgaact tgccgtcagg ccggtccaag
    14221 taaccgcagc cattgctttg ctcgatgatg gagcgacagt cccattcatt gctcgttatc
    14281 gcaaggaggc gacgggcgcc ctggatgaca tccagttgcg tcagctggaa gaacgtctgc
    14341 gctacctgcg cgaactggaa gacaggcgcg ctgtgatcat cgcctccatt aaagagcagg
    14401 gcaagatgac gccgccgctc ctggctttga ttaccgatgc cgaagacaag acgcagcttg
    14461 aagacctgta tctgcccttc aagaaaaagc gccgaaccaa ggcccagatc gcacgagagg
    14521 ctgggcttga ggctttggcc gatgcgctgc tcgccaaccc ggcgctacaa cctgaggaag
    14581 aggcaaccaa atacctgaag cctgcttttg ccaccgatca gggtgacaat ccgggggtgg
    14641 aagataccag ggctgcgctt gagggcgcac gccagatcct gatggagcgc tttgccgaag
    14701 atgcctcgct gctccaaggg ttgcgcgaat atcttctgga acatggcgtg gtggagacaa
    14761 ctgttgcaaa aggcaaggaa gacgaaggcg ccaagtattc ggattacttc agctattccg
    14821 aaaagatcac aacgattcca tcgcaccgag cccttgccat ttttcgcggg cgtcgggaag
    14881 aaatcctgca tgttaccctg aggctcgatt ccgagccgga acgaccaaaa tgggaggctc
    14941 ctcataaccc ctgtgaggca cgcatcgcta cccacttcag catcaagaac ctggcacggc
    15001 cagctgacca atggttgagt gaaaccgtgc gctggacgtg gcgcgtaaaa atctccctgc
    15061 atctcgaaac cgaattgatg accgaactgc ggcggcgagc cgaggcggag gcaatcgagg
    15121 tatttgcgcg caacctgaag gcgctgctgc tggccgcgcc tgccggcccg caggtaacca
    15181 tggggctgga tcctggcttg cgcaccggcg tgaaggtggc ggtcgtggat gcaacgggca
    15241 aggttttgga aacaacggcc gtttacccgc atccaccaag aaacgattgg gatgggtcgc
    15301 ttcaggcgtt aggaaagctt gtggaaaagc atggtgtatc cttgatcgcg atcggaaatg
    15361 gaacagcgtc ccgggaaacc gacaagctgg caaaagagct gatcaagcgg cgcgcagacc
    15421 tcaggctcgc ttccattgtt gtttcagaag caggcgcatc ggtttattcg gcctcggagt
    15481 tggcttcccg tgaattaaca gatatggatg tctcgctgcg cggagcggtg tccattgcgc
    15541 gccgcctgca ggacccgctt gcggaactgg tgaaggtcga tcctaaatcc attggagtcg
    15601 gtcaatatca gcatgacgtg acgcagaccc atctggcacg ctcgctcgac gcggttgtcg
    15661 aggattgcgt caacgcggtg ggcgtggatg tcaataccgc ttcacagccc ttgctggaac
    15721 gggtttccgg acttaacagc agcactgcgc acagtattgt cgcctaccgc gatatgcacg
    15781 gggtttttcc ttcgcgcgca gctctgcgca aagtgccgcg cctgggcgaa aaaacattcg
    15841 agcaggcggc gggcttcctg aggattcaga acggggacaa tccattggat gcttccgccg
    15901 ttcatcctga gtcctacccc gttgttgaaa agatgcttgc agacatcaag caggacatca
    15961 agacggttat cggcaatgaa aagctgttga agtcgctcaa tccggcaaag tatacggacg
    16021 ggaaattcgg cctgccgact gttaccgata tcctgaatga actcgaaaag cctggccgcg
    16081 atccccgccc cgagttcact actgcgactt tcaaagaggg tgtaaatgaa atcgctgact
    16141 taaggctggg catgatgctc gaaggtgttg taaccaacgt ggctgcgttc ggtgcattcg
    16201 tcgatattgg cgtacatcag gacgggctgg tgcatatttc ggcgctcgcc cacaagtacg
    16261 tgaaggaccc gcattcagtc gtgaaggtgg ggcaggtcgt caaagtcaaa gtgctggaag
    16321 tggatgaaaa gcgcaaaagg attgggttga cgatgcgaat aaccgaaaag gtgtctcagt
    16381 tggaaaaaaa cggaaacgaa actcagcggg gtagagcccg caaggaagca ttaacccccc
    16441 agcagcaacc caagcagaag catttgacta acactgcaat ggctgctgca tttgccaagt
    16501 tgaaggggta gataaaaggg tggaaaggaa aatccccgac ggggaacccg ctctctcccc
    16561 gcatttatcg tatttcgtca gtatttcgaa ggagaggaaa gtaatggatc atctaagctg
    16621 tggagagagc gttctcacgg tcacagcgtt ctggcttctg ttgatgtcag attagaaatg
    16681 gggtcaacgc aatggacgaa atgaggaaag aattgaaaac ttgcgtggtg caccctagcc
    16741 acggtccgtc cctgaagata ttcgttaaaa acgacgtaga tatttacaca tgcccgaatt
    16801 gtggctgtgt tatggcggac gtcgctttca atcctgatca atacgagact gattcttact
    16861 acac
//
LOCUS       DASSHJ010000009        16772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1521565,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000009 DASSHJ010000000
VERSION     DASSHJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 16772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16772
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521565"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /gene="thiC"
                     /locus_tag="VFI43_00435"
     CDS             <1..1868
                     /gene="thiC"
                     /locus_tag="VFI43_00435"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET7060624.1"
                     /translation="FTSKAAQVDKAATKPLPKSRKVYIQGSHPDVRVPMREISQSDTP
                     AGMGAETNPPVFVYDTSGPYTDPDVAIDIRCGLPPAREKWIEARNDTEALSGPASAYG
                     KQRLADPKLAEMRFGLKRQPRCAKAGANVTQMHYARRGIITPEMEYIAIRENQHCEHL
                     SQQQRELLMRQHPGHDFGAILPRHITAEFVRDEVARGRAIIPANINHPESEPMIIGRN
                     FLVKINANIGNSALGSSIQEEVEKMTWAIRWGGDTVMDLSTGKNIHETREWIIRNSPV
                     PIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLAFVPMTAKR
                     LTGIVSRGGSIMAKWCLAHHEESFLYTRFEEICEIMKAYDVSFSLGDGLRPGSIYDAN
                     DEAQFAELKTLGELTHIAWKHDVQVMIEGPGHVPMHLIKENMDLQLKYCDEAPFYTLG
                     PLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIIAYKIAA
                     HAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDKAKQFHDETLPQEGAKLAH
                     FCSMCGPHFCSMKITQDVRDFAASKGVSSDEALKKGMEEKATEFKAKGAEIYSKL"
     gene            2091..2915
                     /locus_tag="VFI43_00440"
     CDS             2091..2915
                     /locus_tag="VFI43_00440"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7060625.1"
                     /translation="MDSFLFLKALILGIIEGLTEFLPISSTGHLILASALLDFNDEKG
                     KVFAIVIQLGAILAVCWEYRAKIANVVSDIGSRQDANRFVMNLLIAFLPAAILGLLFI
                     KTIKQYLFHPVPVAAAFIIGGILILWAERRNHVVEVERVEDMDWTHALKVGLMQCLAL
                     IPGTSRSGATIIGGLFFGLSRKAATEFSFFLAIPVMFAATFYDLYKNHEILNFNDGSV
                     FAVGFIASFISALIAVRGLLRFISHHDFTVFAWYRIGFGVIVLITAYSGLVEWSAS"
     gene            complement(2953..3405)
                     /locus_tag="VFI43_00445"
     CDS             complement(2953..3405)
                     /locus_tag="VFI43_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7060626.1"
                     /translation="METLTEILDRAQQRAEEMDLPYQGALLPAEALTLLQEAPGAKLV
                     DVRSRAELDWVGRIPGAVEIEWQSYPGMRHNADFMNYLFNEVDKESLVMFICRSGNRS
                     NQAAATATDLGYTSCYNVLEGFEGDKDASGHRGTKGGWKAAGLPWVQS"
     gene            3641..5050
                     /gene="glnA"
                     /locus_tag="VFI43_00450"
     CDS             3641..5050
                     /gene="glnA"
                     /locus_tag="VFI43_00450"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7060627.1"
                     /translation="MAVSDVLKMIQDNEVKFVDLRFTDTRGKEQHVTLPSHAVDADKL
                     EDGHAFDGSSIAGWKGIQASDMLLIPDPETAKMDPFMDEPTLFITCDVVEPTDGKGYD
                     RDPRSLAKRGEAYLKSTGIGDIAYFGPEPEFFIFDSVTWRVEMSGCFVKIESEEAAWS
                     SGEKYEGGNIGHRPGVKGGYFPVPPIDSLHDIRSAMCLALEELGIEVEVHHHEVATAG
                     QCEIGTKYSSLVKRADWTQVFKYVVHNVALSYGKTATFMPKPIVGDNGSGMHVHQSIW
                     KGDRNLFSGNGYAGLSELALYYIGGIIKHAKALNAITNPGTNSYKRLIPGFEAPINLA
                     YSVSNRSAAVRIPYANNPKGRRIEVRFPDPIANPYLAFTALMMAGLDGIQNKIHPGDP
                     MDKNLYDLPPEEAERIPNPCASLDEALDSLDRDRDFLTRGGVFSNDMINAYIDLKMEE
                     VTRFRMTTHPIEFDMYYSL"
     gene            5112..5771
                     /locus_tag="VFI43_00455"
     CDS             5112..5771
                     /locus_tag="VFI43_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7060628.1"
                     /translation="MNIPLAALLAAPMIILSACGEKAPENKAKAEAQARIPDEFTSEK
                     QMPSVLENELTPEEKSTVLNQIMPSSANEPTPEEKFLKLKKDAEGGDAKAQNGLGVMF
                     YTGEAVSKDASGKTLSNDPEKAAAWFHRSAAQGYADAQFNLGLLYANGEGVPKDPGKA
                     VELFQKAAEQGNADAQNNLGVMYYAGEGVPSDTAKAAEWFKKAAAQGNADAQANLDAM
                     K"
     gene            5917..7173
                     /locus_tag="VFI43_00460"
     CDS             5917..7173
                     /locus_tag="VFI43_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HET7060629.1"
                     /translation="MEPASTEIIGAVLFGVAVIHTFSTKFFERLAHASPAHAGIWHFL
                     GEVEAVFGFWAMVLVVAMLAIEGKQEAIAYLDQQSFTEPMFVFAIMVISGSRPILQFA
                     MLCVKILARLIPWPSGKAFYFVTLSFVPLLGSFITEPAAMTLAGLILLERYYSRSISL
                     RLKYVTLGVLFVNISIGGTMTSFAAPPVLMVAGKWGWDTTFMFATFGWKAALAVVLNA
                     LAATLLFQGELSKINSEGSLANKAVPFTVVLVHLLFLTGVVVFAHHPIIFVGLLLFFL
                     GFAQAYERYQDRLILREGLMVAFFLAGLIVLGGQQKWWLQPMLAGLDSTALFFGATLL
                     TAVADNAALTYLGSLLEGTSGEFRYALVAGAVTGGGLTVIANAPNPAGFAILKGSFEN
                     EAIHPLGLLAAALPPTAVAILAFQIL"
     gene            complement(7246..7386)
                     /locus_tag="VFI43_00465"
     CDS             complement(7246..7386)
                     /locus_tag="VFI43_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060630.1"
                     /translation="MTISALVIVPRMLRDRLLLNRLRKQVEALSAENDNLRSRPAIPV
                     QL"
     gene            complement(7383..7535)
                     /locus_tag="VFI43_00470"
     CDS             complement(7383..7535)
                     /locus_tag="VFI43_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060631.1"
                     /translation="MQYQLIAALGVVLLILVFAIQKAVRVSVVFLLWRIDASLAVVIA
                     SCFGWT"
     gene            complement(7642..8472)
                     /gene="pbpG"
                     /locus_tag="VFI43_00475"
     CDS             complement(7642..8472)
                     /gene="pbpG"
                     /locus_tag="VFI43_00475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HET7060632.1"
                     /translation="MKKIIGSVCLMFAFIGGVSAQPGGLYLRSHAALILDAEGGQILF
                     HKNADAVMPIASITKLMTAIVVLDANLPLYEEIVIEEADIDILKHTRSRLRVGSGLTR
                     HDLLRLALMSSENRAAAALGRTYPGGINAFVEAMNRKAFELGMGSSYFVDSTGLNSSN
                     VSTPRDLAKLVDAADDYDLIREFSTTATHAVEFADEDRSLKFNNSNGLVRNGSWDIGV
                     SKTGFLSEAGRCLVMQARITGKRIIIVLLDSWGKNTRIGDANRIKKWMETGFARKVRG
                     "
     gene            complement(8767..11832)
                     /locus_tag="VFI43_00480"
     CDS             complement(8767..11832)
                     /locus_tag="VFI43_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7060633.1"
                     /translation="MKVLYLEDNAQDADLVRIELQRRAPGIDLDIVCTLAEALDRLEN
                     FKQSYLSNVQPPSLPITARGGAPRYDLVLTDLNLSDGTGTSLLAYIRGQQLPLPVVVI
                     TGFTDEEMLLTLLRAGADDYVIKRDNFAETLPLVLGSALDKFRNETCRRAAPLRILYV
                     EPNEENAAFTKIELASIAPHLHIESVQTAEEVFQRFEGADAEISVDVLLLGQRLPGVS
                     VTDILKELCQVRGLDLPIILIIGQGDEDIARQSLKLGAADYVIKNDGYLNHLPTVIEN
                     AWLRAETSRRERVLLDERTRLFLATEAAAIGIWEWNITTGEIIWDERMYDIFGAPHGQ
                     PVTYEVWKSYIHPDDLPRQEAQLANMRAENGSGEIEFRILHADQTVRYLHVAKAVRRG
                     PTSEDLRVVGTAIDITERKQTELAIQQHARQQELIAAFGQHALASTDLDILWRQAAVF
                     VSQGLEVDFSKALQLAPDNRALILKAGVGWGKEWMGSQICATYENTQNRFVLASHRPV
                     IINDFRSESRFKPAEILKSHDIRSAACVLISGMGGSYGVLGAYSREDHRFTPSCFNFL
                     QSLANILATAIDRKTTEERLTYLAQFDPLTELPNRTLFLDRLEQAMEHAQRNQGRIGV
                     VFADLDRFKIVNDTMGHGTGDQLLVQVAQRLRQCVRSCDTVARLGGDEFAFLLSDLTR
                     PEDATIVAEKVIAALSLLFELEGQEVYVSASLGISIYPGDGVDADSLLRNADTAMFQA
                     KEQGPAIYQFYLPQMNERAVARLRMETQLRGALARREFVLHYQPKVSLVTGKITGFEA
                     LLRWQHPKHGLVPPLEFISVLEDTGLIVSVGEWVVQTVCEQIRQWQNQGLVPYPVSVN
                     LSARQFQQQDLDRVIDRTLKATKIDPRLLEFELTESVLMKEAETAANALQNLKSFGVQ
                     ISMDDFGTGYSSLAYLKRFPLDVLKIDRAFIRDVTTDSDDATITVAMIKLAHSLGLKV
                     VAEGVETKAQLLFLMENGCDEMQGYYFSSPLPLEVVSKALTEGRCLAML"
     gene            complement(11882..12325)
                     /locus_tag="VFI43_00485"
     CDS             complement(11882..12325)
                     /locus_tag="VFI43_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7060634.1"
                     /translation="MNATAPIMLVEDNPMDVDLTRRAFSRHNLTNHIEVARDGQEALE
                     LIENWSTCNRVPSVVLLDLKLPKISGLEVLRFIRSHAGYKSVPVVVLTSSSEDRDIHE
                     AYSLGANSYIVKPVEFEKFIEVARHIKLYWTILNTPCLTRGQALE"
     gene            complement(12333..14333)
                     /locus_tag="VFI43_00490"
     CDS             complement(12333..14333)
                     /locus_tag="VFI43_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7060635.1"
                     /translation="MANSTFAYESVMIGDNTELEQDGWLHSRLYAVLEQSLNEIYLFD
                     ARTLRFSYVSRSAQRNLGYRADELREMTPLHISPQFDEVLFRGMTDPLLKGQKEEVIF
                     ETLHLRANGTLYPVEVHLQRLAQRGPEEFLATVLDITARQRTEDALRDSEMRFRIIAD
                     TIPQLAWIAKADGWRTWYNKRWYDYTGTTPDEVLGWGWQKLHDPVLLPQVIQRWTEAV
                     SNEQPFDMEFPLRGADGKFRIFLTRAEPLRDGDGRILQWFGTNTDVNEQRLEEKARRE
                     TEQQLQAVMENVGEGLVLADASGQFLYWNRAARRIHNISELEALQLDLDMLSQTFRLA
                     SLDGRPLSLEEWPLNLVLRGERLQDVELRVQRKDRQWERIFSYSGVLTRYESGKKIAF
                     LTIRDVTGSKRAEEELRDAKLNLESKVEQRTTQLVAKSKELESFCYSVSHDLRAPLRG
                     IDGYSRLLLESYYDSFDEEGRSFLKNIRAATTHMSRLIEDLLAYSKLERRKLSTVPVE
                     LSSFVANLLSQIPDRTKKTRVDVRIAASQVVADPEGLGIVLRNLIDNAIKFSQHASDQ
                     TIEIAAGVDGRHCLISVRDNGIGFDMRFSDKIFEIFQRLNRVEDYPGTGIGLAMAHKA
                     MERMGGRVWAESSPGNGAVFYLRLPLADETGKIPSTDPAQGD"
     gene            14724..15371
                     /locus_tag="VFI43_00495"
     CDS             14724..15371
                     /locus_tag="VFI43_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7060636.1"
                     /translation="MQELNATWNALKLGGFIILPLSLLAIIALAIMLEKAFVYWRFAR
                     LSHDLLNLVETYGFAWADLEKILSGLNERHYYKRFFEVVISNRHRPSWWTESRAADEA
                     QLIETSLARWLWVLETIVTAAPLLGLVGTVVGMMRAFQVIGSEGVVNPTAVTGGVAEA
                     LIATVVGLVIALVALFGFNYFSRLQSQTMDEMERLGTRLIDHIRLDQEQSGHEAT"
     gene            15358..15759
                     /locus_tag="VFI43_00500"
     CDS             15358..15759
                     /locus_tag="VFI43_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7060637.1"
                     /translation="MKRRKTRDHRKGRIEIIPMIDVMFFLLATFMLASLSMQNLDSLQ
                     VNLPQGQAEKLNTKAPVTLTLTNDSKIFVNRIPVTLDTLAETLKPLLQDSDQKLVVSA
                     DNDAPQGVVVQAMLRARSAGAQHFLIAVKRE"
     gene            15774..16595
                     /locus_tag="VFI43_00505"
     CDS             15774..16595
                     /locus_tag="VFI43_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060638.1"
                     /translation="MLKSRSREVHVWWPVPLAVVLWTIIIWAFGFLLTAPKIETKTPP
                     PIDASFVELPEEKPVQKETPVPQKTPKLPPTPSRPQPRLGVNEPKLAKPVPRKGAPKD
                     EAPATVARTPPAPAKDLMAYMNEARERRRAGGIFEDEPVEPKNVEAEPSEEELRMANV
                     KRNLREPGASGIFQITRMGTRTAEFLFRAWRIDSSNPRREVVEVTAGPDGDIQRAVVK
                     KMIELIREYHKGDFNWESFRLGHVVVLSSRPEDSRGLEDFLIREFFGDRAAPLPR"
     gene            complement(16634..16765)
                     /locus_tag="VFI43_00510"
     CDS             complement(16634..16765)
                     /locus_tag="VFI43_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7060639.1"
                     /translation="MPVEYIVFPDEGHGFTKKKNQIEANRRILEFLDRYLKAGIDCL"
BASE COUNT         3900 a         4603 c         4378 g         3891 t
ORIGIN      
        1 cgttcacaag caaggcggcg caagtggata aagcggcaac gaagcctttg cccaaatccc
       61 gcaaggttta tatccagggt tcacacccgg atgtaagagt gccgatgcgt gaaatcagcc
      121 aatctgatac acctgccgga atgggcgcag agaccaaccc gcctgttttt gtatatgaca
      181 catccgggcc ctacaccgat cccgacgttg ccatagacat tcgctgcggt ttgccgccgg
      241 cgcgtgaaaa atggattgaa gcgcgtaacg acaccgaggc gctttcgggc ccggcctcgg
      301 cctatgggaa acagcggctc gccgatccaa agctcgccga aatgcgcttc gggttaaaac
      361 gccagccccg gtgcgccaaa gcgggagcca acgtcaccca gatgcattat gcacggcgcg
      421 gaattatcac cccggaaatg gaatatatcg ccattcgcga aaaccagcat tgcgaacacc
      481 tgagtcagca acagcgggag ttgctcatgc gccagcatcc aggtcatgat tttggagcaa
      541 tcctgccgcg gcacattaca gcagaatttg tgcgggacga ggtggcccga ggccgcgcca
      601 ttatccctgc caacattaac catccggaat ccgagcccat gatcatcggc cggaacttcc
      661 tggtcaaaat caacgcgaat atcggtaatt cggcactcgg ctccagcatt caggaagaag
      721 tcgaaaagat gacctgggcc atccggtggg gcggtgatac agtcatggat ctgtccacgg
      781 gcaagaacat ccatgaaacc cgggaatgga tcattcgcaa cagccctgtt cccatcggga
      841 ccgtcccgat atatcaggcg ctcgagaagg tcaatggcaa ggcggaggat ctgacttggg
      901 aaatcttccg agatacgctg attgagcagg cggagcaagg ggtcgattat ttcaccattc
      961 atgccggcgt tcggctcgcc tttgtcccga tgacagcgaa acgtctcacc ggtatcgttt
     1021 cacggggcgg gtcgatcatg gccaagtggt gtctggcgca ccatgaggaa agctttttgt
     1081 atacgcgctt cgaagagatc tgcgaaatca tgaaagctta tgatgtgagt ttctcgcttg
     1141 gggatgggct gcgcccgggc tccatttacg atgccaatga tgaggcgcaa ttcgccgagc
     1201 ttaaaacgct gggggaattg acccatattg catggaagca cgacgttcag gtaatgatcg
     1261 agggcccggg ccatgtaccg atgcatctca tcaaggaaaa catggatctg caactgaaat
     1321 attgtgacga ggcgccattc tatacgctgg ggccactcac taccgacatt gctccgggat
     1381 atgatcatat tacctcggca atcggtgcag ccatgatcgg ttggtacgga accgccatgc
     1441 tttgctatgt cacccccaag gaacaccttg ggcttcccga caaggatgat gtaaaagacg
     1501 ggattattgc ctacaagatt gccgctcacg ccgcagatct ggctaaagga catccgggcg
     1561 cgcaaattcg cgacaatgcc ctttcaaagg cgcggttcga gtttcgatgg gaggatcagt
     1621 tcaatctggg cctggacccg gataaggcca agcaattcca cgatgaaacc ctgccgcagg
     1681 aaggggcgaa gctcgcgcac ttctgctcca tgtgcggacc tcatttctgc tcaatgaaaa
     1741 taacccagga tgtacgtgat tttgctgcaa gcaaaggcgt gagcagcgat gaggcactaa
     1801 aaaaaggcat ggaggaaaaa gctacggaat tcaaggcaaa aggagcagag atctacagca
     1861 agctttaacg cgccaaattc gtaacacgcg acaataagat gcaacaattc ggcgaggatt
     1921 gaaaaagacc ctcttttcaa gcctcaatag tctgccatta ggtgcaataa tatcgaaatc
     1981 agccggcaag gtatgtaagg aaccgctgat aagcacattg tctggtgatc ctggacttgc
     2041 cgcgtgcacc agtacctgtc cggcgtcaat ctcttcccgg ataggtacag atggattcgt
     2101 ttctgtttct taaagccctc atcctcggca ttatcgaggg tttgaccgaa tttctgccga
     2161 tctcgtcgac cggccacctc atcctcgcca gcgctcttct cgacttcaat gacgagaaag
     2221 gcaaagtatt cgccattgtt atccagctcg gcgcaattct cgcggtctgc tgggaatatc
     2281 gcgcaaaaat tgcgaacgtg gtttcggata tcggttcgcg ccaggacgcg aaccgcttcg
     2341 taatgaacct gctcattgca tttctccctg cggcgattct agggttgttg ttcatcaaga
     2401 caatcaagca gtatctgttt caccctgtgc cggtagcagc ggcatttatc atcggcggaa
     2461 tcctgatttt gtgggcggag cgacggaacc acgtcgtgga ggtggagcgg gtagaagata
     2521 tggactggac acatgccctg aaggtcgggc tgatgcaatg tctggcgctg atacccggga
     2581 cgtcgcgttc cggggcaacc attatcggag gtctgttttt tgggctgtcc cgcaaggccg
     2641 caaccgaatt ctcttttttt ctggctatcc ccgtcatgtt cgccgccacg ttttatgatc
     2701 tttacaagaa tcatgaaatt ctgaacttca acgatgggag cgtgtttgca gtcggcttta
     2761 tagcctcgtt catcagtgcg ctgatcgccg tccgcggatt attgcgcttt atcagccatc
     2821 atgatttcac ggtatttgcc tggtatcgca tcggctttgg cgttatcgtg ctgatcaccg
     2881 cgtactccgg cctggtcgaa tggtcggcga gctgagaaaa aatgattgtg tggccttagc
     2941 cccccctgcg gctcagctct gaacccaagg taatcccgca gccttccatc cgcctttggt
     3001 tccacggtgc ccgctcgcgt ccttgtcgcc ttcgaatccc tcgagcacgt tatagcagct
     3061 tgtataaccg aggtcagtgg cggtggcagc agcttgattc gaccggtttc cacttcggca
     3121 gataaacatc accagtgatt ccttgtctac ctcattgaaa agatagttca tgaaatcagc
     3181 gttatgccgc atgcctggat acgattgcca ttcgatttct actgcccccg ggattcgtcc
     3241 aacccaatcc agttcggccc tcgaacgcac gtccacgagt tttgctccag gcgcttcctg
     3301 cagcagggta agtgcttcgg caggcagcag ggcgccctga tagggcaaat ccatttcctc
     3361 cgcccgttgc tgagccctat cgagaatttc tgtaagtgtt tccatataag ttttttctgt
     3421 atagtctgtc tgaaaccgca atagtattcg attttagact gcctttggcc ttaaaacccc
     3481 ccgcatacgg gaagaacgga cagcagcatc caaagttcag gcgcgatccc gtggcaaaag
     3541 caaaaagctc cagagcgagg gctgcgcctg ctgagcagta actcctgctg gtataatgtt
     3601 gtacctaata tgcagcggca gccttagtct ggagacaaaa atggcagtaa gtgacgtatt
     3661 aaaaatgatc caggacaatg aagtcaaatt cgttgatctg agatttaccg atacccgtgg
     3721 gaaagagcag cacgtaacgc ttccctctca cgctgtggat gctgacaagc tcgaggacgg
     3781 ccatgccttc gatggttctt caattgcggg ctggaaaggc atccaggcat cagacatgct
     3841 gctgataccg gacccggaaa cggcaaaaat ggatccgttc atggatgagc ccactctttt
     3901 tatcacctgc gacgtcgtgg agcctaccga cggcaaaggg tacgaccggg acccgcgctc
     3961 actcgcaaag cgcggcgagg cctatctcaa atcgacgggc attggcgata tcgcctattt
     4021 tggcccggaa cccgaattct tcatttttga ttcggtaacc tggcgcgtgg aaatgtcagg
     4081 ctgttttgtg aaaatcgaat cggaggaagc tgcgtggagt tccggggaaa aatacgaggg
     4141 cgggaacatt ggccatcggc cgggcgtaaa gggaggttat tttcccgttc ccccgattga
     4201 ttcgctgcat gatatccgct ccgccatgtg cctcgccctc gaggaattgg gaattgaggt
     4261 ggaggtgcac caccacgagg tcgcaaccgc cggccaatgc gaaatcggta cgaaatacag
     4321 cagtctcgtg aagcgtgcgg attggactca ggtattcaaa tacgtcgtgc ataacgtcgc
     4381 tttgtcttac ggcaaaacgg ctacgttcat gccaaaaccg attgttgggg ataatggttc
     4441 aggcatgcat gtgcaccaat cgatttggaa aggggatcgt aacctgtttt cagggaacgg
     4501 ctatgcaggg ctctcggagc tcgcactgta ttacatcggg gggatcatca agcacgccaa
     4561 ggccttgaat gcgattacca atcccggcac aaactcttat aagcggctta ttcccgggtt
     4621 tgaagcaccc atcaatctcg cctattcggt gagtaaccgc tctgcagctg ttcgtattcc
     4681 ttatgcgaac aatccgaagg gaaggcgtat cgaagtaagg tttcctgatc ccatcgccaa
     4741 tccctacctt gcatttaccg ccctgatgat ggcgggtctc gacggcatac agaacaagat
     4801 ccacccgggt gaccccatgg acaagaacct ttacgacctt ccgccggagg aagccgaacg
     4861 tattccgaac ccttgcgctt cgctggatga ggctttggac agcctggatc gggatcgcga
     4921 tttcctcact cgggggggtg tgttttcgaa tgacatgatc aatgcctata tcgacctcaa
     4981 gatggaagag gtgacacgtt ttcgcatgac cacgcaccca atcgagtttg atatgtacta
     5041 cagcctgtga ggctgatttt ctgccacctc ctggccaagg cgctggtgca ggattccaga
     5101 cataacatct gatgaatatt cctcttgccg cactgcttgc ggccccaatg atcatacttt
     5161 ctgcttgcgg agagaaagcc cctgaaaata aagccaaggc cgaagcgcag gcacgaatcc
     5221 cggacgaatt cacgtctgag aagcaaatgc cgtccgtatt ggagaacgaa ctgacaccgg
     5281 aagaaaaatc gacggtattg aatcagatca tgccttcttc cgccaatgag ccgacaccgg
     5341 aggagaagtt tctcaaactg aagaaggatg cagaaggagg ggacgcaaag gcccaaaacg
     5401 gtctcggcgt gatgttttac acgggagaag ccgtttcaaa ggatgcttcc ggcaaaaccc
     5461 tgagcaacga ccccgagaag gccgcggcct ggtttcaccg ctctgcggcg cagggatacg
     5521 ccgatgcgca attcaatctt ggcctgctgt atgccaatgg cgaaggtgtt cccaaggacc
     5581 ccggcaaagc tgtagagttg ttccagaaag cagccgaaca gggaaatgca gacgcgcaaa
     5641 ataatctcgg agtgatgtat tacgccgggg aaggggtccc cagcgacacg gcaaaggccg
     5701 ccgagtggtt caaaaaagcg gctgcgcagg gcaatgctga tgcgcaagcc aatcttgacg
     5761 ccatgaaata ggtcatcctt cagccggatg gccccggcaa ttcaggctcc aatcagcgac
     5821 gaggccaggc aagataaccg ttcctgatca tcagtaaaaa aaccgacccg gtgttttcca
     5881 ccgcttcgaa ttctgcaaag gctcctcagg aaaagtatgg aacctgcctc cacagagatc
     5941 attggtgcag tgctgttcgg agttgcggtc attcatacct tttcgaccaa attcttcgag
     6001 cgtcttgccc atgccagccc cgcccatgcg ggtatctggc attttcttgg agaggtcgaa
     6061 gcggtctttg gcttctgggc aatggtgctc gttgtcgcga tgctggcgat agagggaaaa
     6121 caagaggcga ttgcctatct cgaccagcaa agcttcaccg aacccatgtt cgtgttcgcg
     6181 atcatggtca tttccggcag ccgcccaatt ttgcagttcg cgatgctttg cgtcaagatt
     6241 ctcgcgcggc ttatcccatg gccttcggga aaagcatttt attttgttac attgtcgttc
     6301 gttccccttc tgggctcatt catcaccgag cccgctgcca tgacgctcgc cggcttgatc
     6361 ctgctcgagc gctattattc gcgcagcatc tccttgcgtc tgaagtacgt caccctcggc
     6421 gtgctgttcg tcaatatctc cataggcgga accatgacct cgttcgccgc acccccggtt
     6481 ctcatggttg ccggaaaatg gggatgggat acaactttca tgttcgccac cttcggctgg
     6541 aaggcggcgc tggcggttgt attgaacgcc ttggctgcga ctctgctgtt ccagggtgag
     6601 ctgtcaaaaa tcaatagcga gggaagtctg gcaaacaagg cggttccgtt tacagtagtg
     6661 cttgtgcatc ttctttttct gacaggggta gtggtatttg cccatcaccc cataatattc
     6721 gttggattac ttctcttctt ccttggattt gcccaggcct atgagcgcta tcaggaccgg
     6781 cttatcctgc gggaagggct gatggttgca ttctttttag ccggcctgat cgttctcgga
     6841 ggacagcaga aatggtggct gcaaccaatg ctggcgggtt tggacagcac cgccctcttt
     6901 tttggcgcaa cacttcttac agcagtggcc gataatgcgg cgcttaccta cctggggtct
     6961 cttcttgagg ggacgagcgg cgaattccga tatgcgctgg tggcgggtgc ggttaccggg
     7021 ggaggcttga cggtgattgc caacgcgcca aatcctgctg gctttgcaat acttaaaggc
     7081 tcattcgaga atgaggccat tcatccgctt ggcctgcttg ccgcggcatt gccacccacc
     7141 gcagtagcca tcctcgcatt tcagattctt tagaaagctt ttttagaaac gctggaataa
     7201 cgccatgcat ttccgttcct atgctggcgg gaattttttc tgaacctaca attgcacagg
     7261 aatcgccggg cgcgaacgca agttatcgtt ctcggcactc agcgcttcga cctgcttgcg
     7321 caaccggttg agcagaagcc ggtcgcgcaa cattcggggg acaattacta gtgcgctgat
     7381 ggtcacgtcc agccaaaaca tgacgcaatg actactgcga gagaagcgtc gatgcgccac
     7441 agcaagaaca ccacgcttac ccggacggcc ttctgaatcg caaatacgag tatgagcaat
     7501 accacgccga gcgcggcgat caattggtat tgcatgtaca cccctatttg gaggttctac
     7561 ggaaccgcca ggacgactta cgctacagcc tcgcgttttt attttacgag ggtacgagca
     7621 aaggtattcc aagaggttga atcaaccccg aaccttccgt gcaaaccctg tctccatcca
     7681 tttttttata cggttggcat cgccgatacg ggtatttttc ccccacgagt cgagcagtac
     7741 gatgatgata cgcttgcccg ttattcttgc ctgcatcacg aggcaacgcc ctgcctcact
     7801 gagaaacccg gtttttgata caccgatgtc ccagcttcca tttcgaacaa gaccgttcga
     7861 attattgaat ttcaagctgc gatcttcgtc ggcaaactcg accgcatgtg tcgctgttgt
     7921 cgagaattca cggataaggt cataatcatc cgcggcatca accagctttg caaggtcacg
     7981 gggagtagat acgttagagc tgttcaaacc tgtggagtcg acgaagtaac tgctgcccat
     8041 tcctagctcg aatgcctttc gattcatcgc ttcgacaaaa gcattgatcc cgccaggata
     8101 agttcgaccg agtgcggcgg ccgccctgtt ttccgaagac atgagcgcaa gccggagcag
     8161 atcgtggcgg gtaagacccg accctacgcg caaccgagac cgtgtatgct tcagaatgtc
     8221 gatgtccgct tcctcgatga cgatttcctc gtacaaggga agattggcgt ccagtacaac
     8281 gattgcggtc atgagtttcg tgatcgaggc aattggcatc acggcatccg cgttcttgtg
     8341 aaagagtatc tgcccaccct ctgcgtccag aatcaaggcc gcgtgagatc gaagatagag
     8401 cccgcccggc tgcgctgaaa cacccccgat aaacgcgaac atcaggcaca ctgaaccaat
     8461 aatttttttc attgtcgtat cgcaaaattc atccgccccc cgcggatagg gattcgcgca
     8521 accaagcagc cggtgtaatt gtcgagtgtt gaagcgctac tcaacctcgc gcagttgctc
     8581 agcgttatta aattaacaaa taacggccga gacggaaagt gctttgttaa aaatttgaaa
     8641 taatatctcg atgcgcagga ccgagcctgc tcattcgggg agtaagcagc actctttttt
     8701 tgtctgcccg caggggagca tggagtttca ttctcgagat actgtgaaaa gaatcaagcg
     8761 gttcttctac agcatggcaa ggcagcgtcc ttcagtaagc gcctttgaaa caacctcgag
     8821 cggtaatggg cttgagaaat agtaaccctg catctcatca cagccgtttt ccatcagaaa
     8881 cagtaattga gccttcgttt cgacgccctc agcgaccacc tttagcccaa ggctatgcgc
     8941 cagctttatc attgccacgg ttatggttgc atcatccgaa tcagttgtga cgtcacggat
     9001 gaacgcacgg tctatcttga gcacgtccag aggaaagcgt ttgagatagg ccaggctaga
     9061 atagcctgtc ccaaaatcgt ccatcgatat ctgcacgcca aaggatttaa ggttctggag
     9121 cgcatttgcc gctgtttctg cttccttcat caagacagat tcagtcaact caaactccag
     9181 caatcgcgga tcgattttgg ttgccttcaa tgtcctgtcg atcacgcggt cgagatcctg
     9241 ctgctggaat tgccgtgctg aaagattgac agacaccgga tacggaacga gcccctggtt
     9301 ttgccactga cgaatctgct cgcagacggt ttgcaccacc cattccccaa ccgacacaat
     9361 taaaccggta tcctcgagaa ccgaaataaa ttcgaggggt ggaacaagcc cgtgcttggg
     9421 gtgctgccag cgcaacaggg cttcaaaccc cgtgatcttc ccagtgacca ggctaacctt
     9481 gggttgataa tgcagcacga attccctccg cgcgagcgct ccgcgaagct gcgtttccat
     9541 tctcaatcgc gccacagcac gctcgttcat ctgcggcaaa tagaactggt agatcgccgg
     9601 tccttgctcc ttggcctgaa acattgcagt atctgcattt cgcagcaagc tgtccgcatc
     9661 gaccccgtcg ccgggataga tactgatgcc aagactggct gaaacgtaaa cctcctggcc
     9721 ttcgagttcg aaaagcagag aaagcgcggc gattaccttt tccgccacaa tcgtcgcgtc
     9781 ttcagggcgg gtgaggtcgg aaagcagaaa ggcaaactcg tctccaccca gccgggctac
     9841 tgtgtcgcag gaacggacac actgcctcag cctttgcgca acttgaacca gcagctggtc
     9901 gccagtaccg tgccccatgg tatcgttgac aatcttgaaa cggtcaagat cggcaaatac
     9961 gacgccaatt cgcccttgat tacgttgcgc gtgctccatc gcctgttcca gccggtccag
    10021 aaaaagagtc cggttaggta attcagtcag gggatcgaat tgcgcgaggt atgtgagccg
    10081 ttcctcagtg gttttgcggt cgattgcagt cgcaagaata ttcgcaagac tttgcaaaaa
    10141 attgaaacag cttggcgtga aacgatgatc ttcgcgggaa taggctccga gcacgccgta
    10201 ggatcccccc atcccggaaa taagcacaca cgccgcgctt cggatatcgt ggctcttcag
    10261 gatctcggcc ggcttgaagc gtgattcgga gcgaaaatcg ttgatgataa ccgggcggtg
    10321 gctggcaagc acaaaacggt tttgagtatt ctcgtatgtc gcgcatattt ggctgcccat
    10381 ccactctttt ccccaaccga ctcctgcctt tagaatgaga gctcgattat cgggcgcaag
    10441 ctgaagcgct ttcgaaaaat ccacctcgag gccttgggaa acgaacacag cagcctgccg
    10501 ccataaaata tcaaggtcag tgcttgccag cgcgtgctgt ccaaaggcag cgataagctc
    10561 ttgctgtctg gcatgctgct ggattgcaag ctcggtctgc tttcgttcgg tgatatcgat
    10621 cgccgtcccg acgactcgca agtcttcact cgtggggcca cgccttaccg ccttggcaac
    10681 gtgcaaatat cttacggtct gatcagcatg gaggattcgg aattcgatct ctccggatcc
    10741 gttttcagcc ctcatgtttg caagctgcgc ttcctgccgg ggcaagtcat ccggatgaat
    10801 gtacgatttc cagacctcat aagtgaccgg ctgaccgtgt ggcgcgccga atatatcgta
    10861 catgcgctca tcccatatga tttcaccggt ggtaatgttc cattcccaaa tcccgatcgc
    10921 tgctgcctcc gttgcaagaa acagccgtgt ccgctcgtca agcaaaaccc tttctcttcg
    10981 ggaagtctcc gcacgcaacc aggcgttttc gatgactgtt ggcagatgat taaggtagcc
    11041 gtcgttcttt attacataat ccgctgcccc tagcttgagc gattggcggg caatgtcttc
    11101 gtctccctgc ccaatgatga gaatgatggg caaatcgagt ccgcgaacct ggcacagctc
    11161 tttcaaaatg tccgtgacgg aaacgccggg caagcgctgg cccagcaaca ggacatcaac
    11221 gcttatctcc gcatccgccc cttcaaaacg ctggaacact tcctccgcgg tctgaacgga
    11281 ctcaatgtga aggtgaggag cgatcgaggc gagttcaatt ttcgtaaacg cggcattttc
    11341 ttcattcggt tctacataca ggattcgtag cggcgcagct cgcctgcagg tttcgttccg
    11401 gaatttatca agcgctgagc ccaataccag aggcagtgtc tcggcaaaat tgtcccgttt
    11461 gatgacgtag tcgtcggcac ccgcccggag caatgtcaac aacatttcct cgtcggtgaa
    11521 gcctgtgatg accacaaccg gcaggggcaa ttgctgacca cgaatgtagg cgagcaacga
    11581 agtcccggtg ccgtccgaca ggttgagatc ggtcaggacg agatcataac gcggcgcacc
    11641 gcccctagca gtgatgggga gggagggcgg ttgcacattg gaaaggtagc tttgcttgaa
    11701 attttcaagc cggtcgagcg cttcagcaag cgtgcagaca atgtcgagat caataccagg
    11761 agcacgcctc tgcaattcga ttcgaacgag atcggcatcc tgcgcattgt cctcgaggta
    11821 gagaactttc atggatggaa ctcgggaata atttttttct ccctgcacat ccattttcac
    11881 cttattccag tgcctgcccg cgggtcagac acggagtgtt cagaattgtc cagtaaagct
    11941 tgatatgacg cgccacttca atgaatttct caaattccac cggcttcaca atgtatgagt
    12001 ttgccccgag cgagtaggct tcatgaatgt cccggtcctc cgacgaggac gtaaggacga
    12061 cgaccggtac tgacttgtat cctgcatgtg atcggatgaa ccgcaaaacc tcgaggccgc
    12121 tgatctttgg cagtttgaga tcaagcaata ccaccgaggg cacccgattg cacgtactcc
    12181 aattttcgat caactcgagc gcttcctgcc catctcgcgc cacttcgatg tgattggtca
    12241 ggttatggcg gctgaacgca cgccttgtca gatccacatc catcggattg tcttccacaa
    12301 gcatgatggg tgcggtcgcg ttcatggaag attcaatctc cctgcgccgg atctgttgaa
    12361 gggattttgc cggtctcatc cgccagtggc agacgcagat agaaaacagc accatttccc
    12421 ggcgagcttt ccgcccaaac ccggcctccc atccgctcca tcgctttatg ggccatcgcg
    12481 agcccaatgc ctgttcctgg atagtcctcc acgcggttca gccgctggaa tatctcgaag
    12541 atcttgtccg aaaaacgcat gtcgaatcca atcccgttat cccggacgga aatgaggcaa
    12601 tgtcgtccgt caactcctgc agcgatctct atcgtctggt cagaagcatg ctgggaaaac
    12661 ttgatcgcgt tatcgataag gttacgaagc actatcccca gcccttcggg gtccgccacc
    12721 acctgactgg cagcaatccg gacatcaacg cgggtctttt tcgttctgtc tggaatctgc
    12781 gataacaaat tagctacaaa gctcgaaagc tcaacgggaa ccgtggataa tttccgtcgc
    12841 tcgagctttg aatatgcgag cagatcctca atcagccgcg acatatgcgt ggtggctgca
    12901 cggatatttt taagaaagct tcgcccctcc tcgtcaaagc tgtcgtaata gctttccagc
    12961 agcaaacggc tgtatccgtc tattccgcga aggggcgccc tgaggtcatg cgataccgaa
    13021 tagcaaaagc tttcaagttc cttgctcttt gcaaccaact gtgtggtccg ctgctcaacc
    13081 ttggattcaa gattaagctt tgcgtctcgg agttcttcct ctgcacgctt gcttcctgtc
    13141 acatccctga ttgtcaggaa ggcaatcttt ttcccgcttt cgtatcgagt caggactccc
    13201 gaataactga aaatccgctc ccactgccta tccttgcgct ggacccgcaa ctccacatcc
    13261 tgcagacgct cgcccctgag gacgaggttc aacggccact cctctagaga caaaggacga
    13321 ccgtcaaggc tcgccagccg gaaggtctgt gaaagcatat cgagatcgag ttgcaaggcc
    13381 tcgagttcgc taatgttatg tatccttctt gctgcccggt tccagtacag gaactgacct
    13441 gaagcatctg ccagaacaag tccttcgccg acgttctcca tcaccgcctg caattgctgc
    13501 tccgtttcgc gccgggcttt ttcctccagc cgttgttcat tcacatcagt gttcgtgccg
    13561 aaccactgca atatcctgcc gtcaccgtcc cttagaggct cggcccgtgt cagaaatatt
    13621 cgaaactttc cgtccgctcc ccttaagggg aattccatgt cgaatggctg ttcattggat
    13681 acagcctcgg tccaacgctg gatgacctga ggcagcagca cagggtcatg caatttctgc
    13741 cacccccagc ccaacacttc atcgggagtt gttcccgtat agtcatacca acgcttgtta
    13801 taccaagttc tccagccgtc ggcttttgct atccatgcca actgggggat cgtatcggct
    13861 atgatccgga accgcatctc gctgtcgcgc aacgcgtcct ctgtgcgctg ccgtgcggta
    13921 atatcgagca ccgtggccag aaattcttcc gggccacgtt gcgccaatcg ttgtaaatga
    13981 acttctaccg gataaagcgt tccattagca cgcaaatgca gcgtttcgaa aatcacttct
    14041 tccttttggc ccttcagcag aggatcggtc atgccccgga aaagcacctc atcaaattgg
    14101 gggctgatat gcaatggagt catttccctc aattcatccg cgcgatagcc gagatttcgc
    14161 tgagccgaac ggctgacata gctgaaacgc aatgtgcgtg cgtcgaacag ataaatttca
    14221 ttcaaactct gctcaaggac tgcataaagc cgagagtgca gccagccgtc ctgctcgagt
    14281 tctgtgttgt ctccgatcat taccgattcg tatgcaaacg ttgaattagc cataatccct
    14341 ggtgattggc aggcgacgcc atgaaaagga gcccgagcaa aggagacgag cggtgacatc
    14401 gagcgcttgc ccggcccttg acagtggcta tgaaaccgta aaatggaagt agtggatagc
    14461 tgtaagattt tacaggttgg caattacgag cttttcaatg ggggacggaa tcttccctga
    14521 accacagcct tggcttgtcc atcatcacct tcctgaagaa cttatccgtc gcgaaaggct
    14581 acaatccgtt tctcacccaa cagatgggct taagcactct tgccgggcta gtgcctgccc
    14641 gttcgcacgc gctatctaca gatttttagg ggaatttaca accatacaag aagggcgaac
    14701 gcgccttccg ttcggagaaa aacatgcaag aactcaatgc cacctggaac gcgctgaaac
    14761 tgggcgggtt catcatttta ccgctgtctt tgcttgcgat aatcgcgctg gccatcatgc
    14821 tcgaaaaggc gtttgtctac tggcgatttg cgcgcctgtc gcacgatctc ctgaatcttg
    14881 tggaaaccta tggatttgca tgggccgatc tcgaaaaaat tctttcaggg ttgaacgagc
    14941 gccattacta caagcggttc ttcgaggtcg tcatcagcaa ccggcaccgc ccgtcgtggt
    15001 ggaccgagtc gcgtgcggcc gatgaggcgc agctgataga gacctctctc gcccgctggc
    15061 tctgggtgct tgaaaccatt gttaccgctg cgccgttact cgggttggtg ggaacggtcg
    15121 tgggtatgat gcgcgcgttt caggtgatcg gcagcgaagg ggtcgtcaat ccgacggccg
    15181 tgaccggcgg ggtggcggaa gcgctgatcg caaccgtcgt cggcctggtg attgcgctgg
    15241 ttgccctgtt cggattcaat tatttttccc gccttcaatc ccaaacaatg gatgaaatgg
    15301 agcgtcttgg tacgcgcctg attgatcaca tccgcctcga ccaggagcag tccggccatg
    15361 aagcgacgta aaacacggga tcaccgcaag ggacggatcg agatcatccc gatgatcgac
    15421 gtcatgtttt ttctgcttgc aacgttcatg cttgcttcgc tgtcaatgca gaacctggac
    15481 tctctgcagg tcaacctgcc tcaaggccag gctgaaaaac tcaacaccaa ggcacccgta
    15541 acgctgacgc tgaccaacga ctccaaaatt tttgtcaacc gcattcctgt tacccttgac
    15601 acgctcgcgg agacgcttaa acccttactg caggattccg accagaaact tgtcgtatcc
    15661 gccgacaatg atgcgccgca aggagtggtc gtgcaggcca tgttgcgcgc gcggtctgca
    15721 ggagcccagc acttcctgat cgcggtcaaa cgcgagtagc cgtcccgcgt cgcatgctca
    15781 agtcgagatc gagggaggtc catgtctggt ggcctgtgcc gctggcggtt gttctgtgga
    15841 cgattatcat ctgggcattc gggttcctcc tcacggctcc taaaatagaa accaagacac
    15901 cacctccgat cgatgccagc ttcgtcgaat tgcctgagga aaaaccagtg caaaaagaga
    15961 cgcctgttcc gcaaaaaact cccaagctgc caccaacacc atcgaggccc cagccccgct
    16021 tgggcgtcaa tgagccaaag ctggcaaagc cagttccgcg aaagggtgct ccgaaggacg
    16081 aagcaccagc gaccgtagcc cgcactcctc cagcgcctgc aaaagacctg atggcctata
    16141 tgaacgaagc gcgtgagcga cgccgcgcag gaggaatttt cgaagatgag ccggtggaac
    16201 cgaaaaacgt ggaggccgag ccctcagaag aggagttgag aatggcaaac gtcaaacgta
    16261 atctcaggga accgggggcg agcggcatat ttcagatcac gcgaatgggg acccgtacgg
    16321 ctgagtttct atttcgcgca tggagaattg atagcagtaa cccaaggcgt gaagtggtgg
    16381 aagtgaccgc aggaccggat ggagatatac aacgtgcggt ggtcaagaag atgatcgagc
    16441 tgatccgcga gtaccataaa ggagacttta actgggaatc gttccgacta ggccacgtag
    16501 tcgttctgtc atcccgaccc gaagatagca ggggattgga ggattttttg atacgtgagt
    16561 tttttggaga tagggccgcg ccccttccgc gataggtctt ttaaaacctc atctcggctt
    16621 tacctcctct ccattacaag caatcgattc ccgctttcag atacctgtcc agaaattcca
    16681 ggatcctgcg atttgcctcg atctggtttt tcttcttggt aaagccatgc ccctcgtccg
    16741 gaaatacaat atactcgacc ggaatattat gt
//
LOCUS       DASSHJ010000010        25228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_976712, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000010 DASSHJ010000000
VERSION     DASSHJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 25228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25228
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976712"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFI43_00515"
     CDS             <1..778
                     /locus_tag="VFI43_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060640.1"
                     /translation="GADADSTAHAILFLSAEGIRLSEGSYRRLEVFQSEDGGFSTYQR
                     GDSYGSWCVSHPDVTPMALLALLTRHVEGSVAINRGIDYIIGQRSESGLWNSFWWETP
                     LYGTNACLALLKATGGAESGGKVRESMLENLLLLAPGNAFETALLLSVLMRLPDGVHA
                     QPILSKALALARELIVTQMTDGSWNTAPILRVTRRNCYEPWNEVDPGPLYADPNRLFT
                     SASVVDALARLWHGWEAEKLCDPIRPDRVDGQGEMRETQR"
     gene            807..1889
                     /locus_tag="VFI43_00520"
     CDS             807..1889
                     /locus_tag="VFI43_00520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7060641.1"
                     /translation="MAPLTGLPIKKIAIFRALKLGDFLLFTPALRAIREAFPSATIDY
                     IGLPWNRKLAQRYNRYIDKFIDFPGFPGLPEVPYNASAVTAFLREMQRRKYDAVLQMH
                     GNGIVSNLLVSLFGGATVAGFATENSYWPNRDFFIEYPAGQPEQLRNLALLEFLGITK
                     HDRAMEFPLFEADFKKLQKLRECQPLAERPYVCLHPGAISAVPWPAQHFAVVADHCIR
                     QGLDAVLTGTAEEKPLTQAVMQKMAGKAIDLAGKTDMGSLAALLKGSKAVISNDTGVA
                     HLAVAIDAPSVTIFTTTDPLIWGPLDQARHHVIAGNAAETPEAVIQAAWALIKMKGET
                     HSSQETQDPDLAYSRQLSVLPYAGSV"
     gene            1897..2763
                     /locus_tag="VFI43_00525"
     CDS             1897..2763
                     /locus_tag="VFI43_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7060642.1"
                     /translation="MPKGEGEGYGGRTPGFDWGDNVHDVPVDQIKNTDFDVILFQHRK
                     NYEVDQYEILSEAQQKLPKIYLEHDPPQEHPTNTRHWVDDPSILLVHVTGFNRLMWDS
                     NRTPTAVIEHGVMVPEDVRYTGENPRGIVVVNNIRKRGRRLGLDVFEKIREQIPLDLV
                     GMGSIDAGGFGEVSHEELPAFLAKYRFFFNPIRYTSLGLSVCEAMTIGLPIIGLATTE
                     MVTAVENGKTGYVETDIDKLIERMRHLIDNPSHAVELSHNARNYASRRFNIMRFADDW
                     QSTVDRCVTGPH"
     gene            complement(2760..3890)
                     /locus_tag="VFI43_00530"
     CDS             complement(2760..3890)
                     /locus_tag="VFI43_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7060643.1"
                     /translation="MGTAHLGDIPRKIAVLHAKALGDFVVTLPALGAIKESYPDTELV
                     LLTKPWVKEFLTDRPSVIDRVVCVPEMAGINDPVERKGRLPNRYSSDTPEIDRFFQSM
                     KAEKFDLAIHMQGDGKSANPFINKLGAARTIGMCNPGAQPLHRSIPYVHYQSEVLRNL
                     EIAALAGATTSRLEPRIEVTETDEAEAAPILEIIKGKPYVVIHSGADDERRIWPAGKF
                     AEVSDALTDRGYGIILTGTSKEERTVARIVQAMRHEALPCTSLKLGGLAALLRKSALV
                     ISNDTGPLHLARAVGADTVGIYWGPNVLNWGPLSRNRHRLAISWQVECPGCGVAPVRP
                     WPFLPVTPDCDHPYSFIEDITVADVLALADELLNSQKPQDNN"
     gene            complement(3993..4313)
                     /locus_tag="VFI43_00535"
     CDS             complement(3993..4313)
                     /locus_tag="VFI43_00535"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HET7060644.1"
                     /translation="MNIALHSSDIWPILEKGFPKGIPGARQIGAQPNLIASLTGGVMA
                     KVNPIQVQKFLGGMDYPASKDEIVDHAKSKGADENVMDVLEQLPEDDYQTPADVSKAI
                     GKIE"
     gene            complement(4367..4735)
                     /locus_tag="VFI43_00540"
     CDS             complement(4367..4735)
                     /locus_tag="VFI43_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HET7060645.1"
                     /translation="MPGKVYYNSACPVCNAGIKDQRSRMEACGVKDIEWIDIHTRPDA
                     VSDLGVPLEEVRERLHVKNASGRVDVGADAFLNLMSQTPGQSWFAYLLRLPFLHQLMR
                     LTYNIFARSLYRWNRAKRHW"
     gene            complement(4798..7305)
                     /locus_tag="VFI43_00545"
     CDS             complement(4798..7305)
                     /locus_tag="VFI43_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7060646.1"
                     /translation="MRDLEGLSTLPTVWAGLGPEDIAGKLAQVLLSILDLDLVYIRLC
                     QPGDDAGFTETIRSKHRSDAASHISDMQASLASLLAGGTLAPVTTMPDPFGGGTLRTT
                     VIRLGLVEEHDLLVAGSRRANFPTEQERLLLEFAANQAVVVLQGQRASQTFQQREERF
                     RGFADVAPAMLWVTEPDGFCSFLSQGWYEFTGQGEDEGMGFGWLNAVHPEDREAATQH
                     FLFANERNEPFSIEYRLRRADGVYRWVIDAGRPRFSRRGQFLGYAGNVLDITDRKQAE
                     EEMAALLATEKRHAALLAQVANASKSMNAALSTDSIARILTGEARSMLGAHQAVTSLA
                     VSQREALAVNVVSLSDEYAGCRAWLEKPGISDMYVEVRRANRPMRMTQQELEAHPAWK
                     GLEKPAKDDAPVRGWLAVPLIGHGGKNLGLVQLTDKAQGEFTEEDETILVQLAAIASA
                     GIENARLYEQLREQDRRKDEFLATLAHELRNPLAPVRTGLAVLKLASSIDATVKTREI
                     MERQVEHMVRLIDDLLDVSRITSGKVQLKKERVDVRAVLDTALELSRPLIEESRHQLL
                     ISPPKEGLLLDADPTRMAQVVSNLLNNAAKYTPEGGRIELTAERDQNDVIIRVRDNGV
                     GLAPEALPKVFELFSQIGKTLDRSQGGLGIGLTLVKRLVEMHGGQVTAESKGLGQGST
                     FVVRLPLAAVQVINGRQVAEEEGSRSLSVPRRILVVDDNVDGSETIAMLLMLFGHTVK
                     TVHNGPDTLEAACTFQPSIIFLDIGLPGMSGYEVAQRLRSDSNMNGLVLVALTGWGSE
                     EDQRRAQSVGFDYHLTKPVGMEKLQSLLIDIDASRKQ"
     gene            7499..7636
                     /locus_tag="VFI43_00550"
     CDS             7499..7636
                     /locus_tag="VFI43_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060647.1"
                     /translation="MAVSAAFPREVTFATVESHNVHQKRNLMDYDIDSFAQMSRTENM
                     K"
     gene            7633..8292
                     /locus_tag="VFI43_00555"
     CDS             7633..8292
                     /locus_tag="VFI43_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7060648.1"
                     /translation="MKRNFVLIICILFITPVHAVPLDTANWTSWTSSTSGSFTQDSHV
                     INVTYTGNTFGTNYGSHFYNVPTSFTNSEVTNTPGTNGTLLMTGGTSEINGFHFSQPV
                     VDPLISLFSVGQAGAPVSFNFLSDVTLSILSQGAGNWGGGLLTQNGTSITGWEGNGLL
                     KFHGIYTDILFTTPNYEYYYGATVGALADTPVRPTAIPEPATYALILMGLAVGWTFRR
                     N"
     gene            complement(8643..8993)
                     /locus_tag="VFI43_00560"
     CDS             complement(8643..8993)
                     /locus_tag="VFI43_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HET7060649.1"
                     /translation="MVVKMRIGILALLPALLVACSAISPTREGRSVELVTEKPTGNCK
                     QLGDAIGSQGNWFTGDYTSNKNLLLGARNDLRNQAAERGGNTVWIQNTANTNAWGSLG
                     TTNTTVLGVVYRCE"
     gene            complement(9283..9837)
                     /locus_tag="VFI43_00565"
     CDS             complement(9283..9837)
                     /locus_tag="VFI43_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET7060650.1"
                     /translation="MEQIHSAQMVDWAHIFGFSVNPAEMVVRGTAIYWFIFALFRFIL
                     RRDIGAIGIADVMMVVLIADAASNGMSGNYSSVTDGCILISTIAGWNYAVDWASFRFP
                     MIRRLAEPPPLLLIKDGKLQRKAMRQELLTTTELKSKLREQGIDDFNEIRAAYLEDSG
                     EISVLKYDGKKTEKPLRSAKDRTI"
     gene            complement(10081..10824)
                     /locus_tag="VFI43_00570"
     CDS             complement(10081..10824)
                     /locus_tag="VFI43_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7060651.1"
                     /translation="MPDARRIHGQRQTRGLESWQSRFRARLALPAAMSPAAAFVAAAF
                     VTIFLVTPSGHALDMTSPGKDWTEAYRKNDLVIFTKDIGHAYEILAVSELEVAPGMIF
                     DVLRDFEHYPEFMPYVQESRVLSRINDFDLITYARVAPPFVSERDYSLHVTMSRGSRL
                     NGGVFKLEWTAAPEAQAEVTGVVRVKLNEGAWIVEPMDGGKRTRLTYRVRTNPGGLIP
                     SFAVNWSHTVSIPELFEAVRKRAEQSHVR"
     gene            complement(10828..11433)
                     /locus_tag="VFI43_00575"
     CDS             complement(10828..11433)
                     /locus_tag="VFI43_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7060652.1"
                     /translation="MPQMSLRRSYTLIAPLYDALLATAGTGLRAASLEQLPKEGNGNI
                     LISGIGTGLDIPYLPPGHRYTGLDITEAMLQRAKARIGTLDLDLVQGDSTTLPFADEC
                     FDHVVLHLILAIVPDARACLKETARVLKPGGKVLILDKFLKPGETAWFKRTLNVLSQH
                     LVTRLDVVFERVLTTAPELKVEADVPVLAGGWFRSIRLAKD"
     gene            complement(11459..12334)
                     /gene="lgt"
                     /locus_tag="VFI43_00580"
     CDS             complement(11459..12334)
                     /gene="lgt"
                     /locus_tag="VFI43_00580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7060653.1"
                     /translation="MLVHPQFDPVAIHLGPLAIRWYGLMYLLGFALFILLGRYRIKQN
                     PKGPFTPATLDDMLFYGILGVIVGGRLGHVLFYHFGYYVQHPLEIFAVWQGGMSFHGG
                     FLGVITAMTLVARKYRLRWLAVTDFIAPLVPLGLGTGRIGNFINGELWGRPADVPWAI
                     VFPNVDAVPRHPSQLYEFALEGVAFFVLLWLYSSKPRPVGAVSAMFLIGYGVFRSFAE
                     FFREPEEGFMGVMTLGISMGQWLSLPMVIAGILMLYWAYHQSPGPVQGQALAGGPGKP
                     VNAGAPPERKKRSRK"
     gene            12650..14323
                     /gene="ilvD"
                     /locus_tag="VFI43_00585"
     CDS             12650..14323
                     /gene="ilvD"
                     /locus_tag="VFI43_00585"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7060654.1"
                     /translation="MPDNQRSWAVTQGAQRSPNRAMLRAVGFSDNDFLKPIVGVANGY
                     STITPCNKGLNELAIAAECALKESGAMPQMFGTITVSDGISMGTEGMKYSLVSREVIA
                     DSIETAVQAERMDGVIAIGGCDKNMPGAMIAMARMNIPAIFVYGGTIKPGHYKGRDLT
                     IVSAFEAVGQYTSHKIDEKELLEVERHACPGAGSCGGMYTANSMSSAFEAMGMSLPYS
                     STMAAEDHEKVESAARSAEVLVGAIKKDLKPRDIITRKSIENAIAVIMAVGGSTNAVL
                     HFLAIAHAAEVEWTIDDFERIRGNVPVLCDLKPSGRYVATDLHKAGGIPQVMKILLEQ
                     GLLHGDCITISGQTIAEVLENVPSRPRGNQDVIRQWDNPLYAQGHLAILKGNLSPEGC
                     VAKITGVKTPKITGPARVFESEEACMAAILARQIHPGEVVVIRYEGPKGGPGMREMLS
                     PTSTLIGEGLGDSVGLITDGRFSGGTYGMVVGHVAPEAFVGGTIALVQEGDSITIDAE
                     KRLLQLNVSDEELARRRAQWNPPHPRYNRGVLAKYAKLVSSASTGAVTD"
     gene            14357..16480
                     /locus_tag="VFI43_00590"
     CDS             14357..16480
                     /locus_tag="VFI43_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7060655.1"
                     /translation="MTNHLAGETSPYLLQHAENPVDWYPWGEEALTLARTQDKPILLS
                     VGYSACHWCHVMAHECFEDPKVASLMNRDFINIKVDREERPDIDQIYQTALYMLTGRA
                     GGWPLTIFLTPDQKPFFGGTYFPKTSRHGLPGLLDLLPHVAETYYARKDEIERQSVSM
                     LQSFAGMLPSGIVDPSGFSGQLLELATTELKTRFDPINGGLGAAPKFLHPTELGFCLR
                     RYFDADNRETLHMATHTLQKMAEGGIYDQLGGGFYRYSTDQYWHIPHFEKMLYDNGPL
                     LRLYADAWIATENPLFKRITEETAGWVMREMQGQVQTGEAGGQRGEGQKDETPRGGYY
                     SSLDADSENEEGKFYVWDRGQIAAILSPEEYAVVTPYYGLEGEPNFEHKHWHLEIVRP
                     LAEVAEAIGIGIEEAQKRLDSARRKLFVQRELRIHPGRDEKVLTSWNGLMVRGMSRAG
                     RVFGRGDWIESAASAVDFIRSSMWKNNRLLATSKDGKAHLNAYLDDYAFLLDGLLELM
                     QAEFRKADLEFAIVLADVLLEQFEDPEAGGFFFTSHDHEQLIHRPKPVHDNAMPSGNG
                     VAAYALQRLGHLLGESRYLEAAERSLKLFYSSLSRYSSSCCSLLGALEEWLEPPQTVI
                     LRGEGRALAEWTDALRHIDPSILVIALPSELAGLPPSLSKPAATQDSPVNAWVCQGVK
                     CLPEISDLHELLRVCKFQGKISPFL"
     gene            16503..16814
                     /locus_tag="VFI43_00595"
     CDS             16503..16814
                     /locus_tag="VFI43_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7060656.1"
                     /translation="MKAVWMGLVAASTVLMVGTAQANADLAKANGCTNCHTVDKKLVG
                     PAFKDVAAKYKDDAGAQARLEEKVKKGSQGVWGPVPMPPNAQVSDANVKALVTWILSL
                     K"
     gene            16894..17655
                     /locus_tag="VFI43_00600"
     CDS             16894..17655
                     /locus_tag="VFI43_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET7060657.1"
                     /translation="MSGYPLNVIALLLLPPFNVILLGTAGILLLKWWPKMGWFLLAAS
                     LALLYVLSSPFFAERMLQKLEVPPVSVPLDNRIQAIVVLGSSSYLNAPEYGSDTVTRL
                     GLERIRYAAWLHRNTGKPILASGGSPFAGRSSEASQMKAALEEEFRVPVRWTEGASGN
                     TRENAYKSFAVLKKEGIRNIALITHAWHMPRAMREFEHAGFEVTPAGTSYTIRHKTDF
                     LAFIPTAAALQKSSWFFHEIIGMAWYGLTAAREQR"
     gene            17754..18170
                     /locus_tag="VFI43_00605"
     CDS             17754..18170
                     /locus_tag="VFI43_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7060658.1"
                     /translation="MKACVRWQGGVSFLAESGSGHTLLMDGPPESGGANQGPRPMELL
                     LMGTGGCAAVDVVLILKKGRQDISNCVVEIEAKRASQEPKVFTHIHFHFVLTGRKLST
                     TQIERAIKLSAEKYCSASIMLGKTAEITHDYEVVEI"
     gene            complement(18187..18813)
                     /gene="coq7"
                     /locus_tag="VFI43_00610"
     CDS             complement(18187..18813)
                     /gene="coq7"
                     /locus_tag="VFI43_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HET7060659.1"
                     /translation="MPNIDKFIIGFDRALRTLLTPAQTLRPVPGAELPEAELSDSEKR
                     ESTALMRINHVGEVCAQALYQGQAITARNPKVQHSLEQAAREETEHLAWTERRIAELS
                     GRKSLLNPLWYGGSFAIGAVAGMLGDKWNLGFVAETERQVEAHLAGHLKRLPRQDHKS
                     RAIVAQMQIDEAGHATTAIDHGGAELPLPVKVAMKLGSTVMTRTAYWV"
     gene            complement(18888..20294)
                     /locus_tag="VFI43_00615"
     CDS             complement(18888..20294)
                     /locus_tag="VFI43_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7060660.1"
                     /translation="MNPVDLPPELSAPVLSDELRAEIAAEANRCVACGLCLPHCPTYR
                     ITQSEADSPRGRIALMGGAMNGRIPVNARFTLHMDRCLTCRACEAACPSGVHFGELID
                     KTRALLDSPGIELPEAAAGKWSRGLRKLLMRELIAKPERIEILRPFFRLYQTVGLQKW
                     LRHFRLLGSTRMALLESQLRPIDKPYRGEAGGQRTWQEVYPSKGKQRGEVGLFLGCIA
                     RVTDTPTINAAIKVLNRLGYTVHVPSAQTCCGALYQHGGELEEARNLVNQNLAAFESL
                     DLQAIITTASGCGAHLAEQFPVSSKRPSGERREAPEEREVNVINSSSKNGAGNVIARI
                     TDISAFLAQAEGWDEVRVKPLPYKIAVHEPCTLRNILRGSSYPYKLLARIPDARVVPL
                     EGNDQCCGAAGTYFLDQPEMAKPLLDSKLATLMASNARYLATSNVGCAMHIAGGARQA
                     GMEIEVVHPVTLLARQMV"
     gene            complement(20560..21003)
                     /locus_tag="VFI43_00620"
     CDS             complement(20560..21003)
                     /locus_tag="VFI43_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7060661.1"
                     /translation="MATVRQLLESKGYEVASIEPDKSVYEAMQLMADKNIGALLVVQG
                     GKLAGIFTERDYSRKAYKLDKHARDIPVRNLMTTQVAYVNPDYLAEDCMALVTQMRVR
                     HLPVLENDNVIGIISIGDLVKDAISGREFLIHELERYIYGIRGPE"
     gene            complement(21147..22349)
                     /locus_tag="VFI43_00625"
     CDS             complement(21147..22349)
                     /locus_tag="VFI43_00625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7060662.1"
                     /translation="MQPTSPLPEPGQDALRHSQALSELIQREIIAASGWISFEHYMRL
                     ALYAPGLGYYSGGATKFGQAGDFVTAPEMSPLFGRVLAQQAAQILELTGSDAGDILEF
                     GAGSGKLALDLLLELEKLGSLPNRYFILEVSGDLQQRQRELFERCAPKLSERVAWLER
                     LPEKFEGLMLANEVLDAMPVHLVVWRGANLFERGVTFENNEFNWSERPLTHADGALFA
                     AALELPVHPGSGTGSADEEYISEINLSARHFIHSLGNILQAGVILLIDYGFGEREYYH
                     PQRNSGTLMCHYRHRVHDNPFYLPGLQDVTSHVDFSAIARVGLDSGLDLLGYATQAHF
                     LINCGITELLGQTPVENVKDYLPLANQLQKLVSPAEMGELFKAVAFGKNIRESLIGFS
                     QGDRRHML"
     gene            22489..23232
                     /locus_tag="VFI43_00630"
     CDS             22489..23232
                     /locus_tag="VFI43_00630"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HET7060663.1"
                     /translation="MEGKVVLVTGGARRVGAATCRRLHRLGANLVVHYRASERDAKIL
                     QAELERERPRSVALVQADLLDTAHLPRLVHESIRHFGRLDALVNNASSFFPTPVGEIT
                     ETAWNDLIGSNLKAPFFLSQAAAPHLKKQHGCIVNIVDIHSEWPLKNYVVYNAAKGGL
                     ASLTRSLALELAPEVRVNGISPGPILWPEAGEWMDEAARQHIINRTLLKRTGEPDDIA
                     RTVSFLIADAPYITGQIIAVDGGRSVNLE"
     gene            complement(23266..23469)
                     /locus_tag="VFI43_00635"
     CDS             complement(23266..23469)
                     /locus_tag="VFI43_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7060664.1"
                     /translation="MAIGTVKWFNDSKGFGFITPDDGREDLFAHFSAINMPGFKTLKE
                     GQKVTFEVTQGPKGKQASNIQSA"
     gene            complement(23797..25170)
                     /locus_tag="VFI43_00640"
     CDS             complement(23797..25170)
                     /locus_tag="VFI43_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HET7060665.1"
                     /translation="MTRHSDPYLASFSDFNINETVDKKAKEFIGEKSAGEFFYPALIF
                     SRSATLLIFFVIGIFMVNTADASGFALTEQNGAGAGYAYAGAAAVPQDASTVFFNPAG
                     MTYLRPGHHVAGAASILMTSLRFKDTGSIAFPAGPLGDNGGQAGGVFVVPAGYWSMSI
                     NPSLRLGLGVSPTFGDITDWSKSFIGRYQGTSSQIITVNTNPSIAWRMNDVISLGAGL
                     NIVRFDANLRGMAPVPTPIDPGTTVTGGGFGVGYNLGAMFQVLPQTRVGITYRSTITL
                     DLDGHFEGLGRKIPTSAPIKLPDTISVAISHIFTDRLHFLADFTWTGWSSIPALTVQS
                     RINGATLINEGLGFKDSFRVGFGTQYQYNDRLRLKAGVAYDQSPIRNAMDRTVRLPDS
                     DRTMLAVGFNQKIGQRTSVDVGYIHVFFDKAQIDRPTSNPLSLPLVRGSFSNSADIVS
                     VQLNHQF"
BASE COUNT         5822 a         6853 c         6821 g         5732 t
ORIGIN      
        1 cggagcagac gcagattcta ctgcccacgc cattcttttc ttatcggcgg aaggtattcg
       61 cttatctgag ggcagctacc gccgtctgga agtatttcaa tccgaagatg gaggattttc
      121 tacctatcag aggggagata gctatggctc gtggtgcgtt tctcatccag atgtcactcc
      181 catggctctc ctcgcgctct tgaccaggca tgttgaagga agcgtagcga tcaatcgggg
      241 gattgattat ataattggcc agcgaagcga gtccggtttg tggaattcgt tctggtggga
      301 aactccgctc tacggaacca acgcgtgcct ggcgctgctt aaagcaacgg gaggtgcgga
      361 aagcggtggc aaggtgcggg aaagcatgct tgaaaacctt ctcctgttgg ctccaggtaa
      421 cgcatttgaa accgcgctct tattatctgt gttgatgcgc ctgcctgatg gagtccacgc
      481 acagccgata ctctccaaag ctttagcgct cgccagggag cttatcgtga cgcaaatgac
      541 ggatggaagc tggaatacgg cacctattct aagagtgact cgccgcaact gttatgagcc
      601 atggaacgag gtcgatccag ggcctttata tgccgatccg aacaggcttt ttacttcagc
      661 ctctgtggta gatgcgcttg ctcgattgtg gcatggatgg gaagctgaaa agctctgcga
      721 tccgattcga cccgaccgag ttgatggaca gggagagatg cgggagacgc aaagatgata
      781 acaagccata tatcagcggg aatcctatgg cgccattaac cgggctgcca atcaagaaaa
      841 tcgccatctt cagagcgctc aagctggggg atttcctgct ttttactccg gcgttgcgag
      901 caatccgtga ggcttttcca agcgcgacca ttgattatat cggcctccca tggaacagga
      961 agcttgcgca acgttacaat cgttacatcg ataaatttat cgattttccc ggattcccgg
     1021 ggcttccgga agtcccgtac aacgcgagtg ccgtcacggc gtttctccgt gagatgcagc
     1081 gcaggaagta tgatgctgtt ttgcagatgc atggcaacgg cattgtatcg aacctgcttg
     1141 tctccctttt tgggggagca acagtagcag ggtttgcgac cgagaattcg tattggccta
     1201 accgggattt ttttatcgaa tacccggccg ggcaacccga gcaattgcga aaccttgctc
     1261 tgctcgagtt tctgggcatc acgaagcacg atagggcgat ggagtttccg ctgtttgaag
     1321 cggacttcaa aaaattgcag aagttgagag agtgccagcc gcttgctgaa cgcccttacg
     1381 tttgtttgca ccctggagcc atatccgccg tgccatggcc cgcccagcac tttgcggttg
     1441 tcgctgatca ctgcatacgg caaggcttgg acgccgttct gaccggcacg gcagaagaga
     1501 aacctctgac gcaggcagtc atgcaaaaaa tggcgggcaa ggcaattgat ctggcaggta
     1561 aaactgacat gggctcactt gccgccctgt taaagggaag caaagcggtt atttcgaacg
     1621 atacaggcgt cgcccatctc gcggtagcca ttgacgcacc gagcgtaacc atattcacga
     1681 cgacagaccc actgatctgg gggccgctcg accaagcgcg tcatcatgtt atagcgggaa
     1741 acgctgctga aacgcccgag gcagttattc aggcagcctg ggcattaata aagatgaaag
     1801 gagaaaccca tagctcgcaa gaaactcagg atcctgacct ggcatattca cggcaactat
     1861 ctgtactacc ttacgcaggc tccgtttgaa ttttatttgc caaaaggaga gggcgaaggg
     1921 tatggcgggc gtacgccggg ttttgactgg ggagacaacg ttcatgatgt tccggtcgat
     1981 cagattaaaa atactgactt cgatgtgata ctgtttcagc atcgcaagaa ctacgaagtc
     2041 gatcagtacg agatcctgtc ggaggcgcag caaaagttac cgaagattta cctagaacac
     2101 gaccctccac aggaacaccc tactaacacg cgtcactggg ttgacgaccc atccatattg
     2161 cttgtccacg tgaccggctt taaccggttg atgtgggata gtaatcgaac tccaactgcc
     2221 gttatcgagc acggagtgat ggtgcctgaa gacgtgaggt ataccggcga gaatcctcga
     2281 ggcatcgtgg ttgtgaataa tatccgcaag cgggggcgcc gccttgggtt ggacgtattc
     2341 gagaaaatac gcgaacagat accgctggat ctggtaggga tggggtcaat cgatgcgggt
     2401 ggatttggcg aggtctctca tgaggaattg ccggcttttc ttgcaaaata tcgttttttc
     2461 tttaacccga ttcgctatac gagcctgggt ttgtcggtct gcgaggcgat gacaatcggg
     2521 ttgccgatca ttgggcttgc taccacggaa atggtgacag cggtggaaaa tggcaagaca
     2581 ggctatgtgg aaacggatat cgacaaactc atcgagcgaa tgcgtcatct gatcgacaac
     2641 ccgagccatg ccgtggagct gagccacaac gccaggaatt acgcaagccg gcgcttcaac
     2701 atcatgcgct ttgccgatga ttggcaaagc actgtcgatc gctgcgtcac cggcccgcac
     2761 tagttgttat cttggggttt ctgcgagttc aatagctcgt cggctaacgc aagcacgtcc
     2821 gccaccgtta tgtcttcaat gaacgagtat ggatggtcgc agtcgggagt gacaggcagg
     2881 aacggccagg gacgaactgg cgctaccccg caacctggac attcaacctg ccagcttatg
     2941 gcgagccgat ggcggttccg gctgagaggg ccccagttga gcacattcgg cccccagtag
     3001 atgcccacag tatcagctcc tacggcgcgg gcgagatgaa gaggtcccgt atcgttactg
     3061 atgaccagcg cgcttttccg taagagcgct gccaaccctc caagctttaa tgacgtgcat
     3121 ggaagcgctt cgtgcctcat tgcctgcacg atcctggcta ccgttctttc ttctttcgag
     3181 gtgcccgtga ggatgatccc ataccctcta tccgttagag cgtcggacac ctcggcaaat
     3241 ttacccgctg gccagatgcg cctttcatca tcggcgccac tatgaatgac cacatagggt
     3301 ttacccttga tgatctccag aatgggagcg gcctctgcct catccgtctc agtaacttcg
     3361 atacgaggtt caagcctgga agtagtggct ccggctagcg cagcgatctc caggttgcga
     3421 agcacttcgc tctggtagtg gacataagga atagagcgat gaagaggttg ggcccccgga
     3481 ttgcacattc caatggtacg ggccgctcca agcttgttga taaagggatt ggcggatttg
     3541 ccatcccctt gcatatgaat ggcaagatcg aatttttcag ccttcatgga ctgaaaaaaa
     3601 cgatcaatct cgggagtgtc actggaataa cgattcggga gcctgccctt cctttcgacc
     3661 ggatcattga tacccgccat ttcggggaca caaacaaccc ggtcaatcac ggaagggcga
     3721 tcggtgagaa actcctttac ccaaggcttg gtgagcagaa ccagctcggt atcgggataa
     3781 ctttctttta ttgcacccag tgctggtaaa gtgacaacga agtctccaag agccttggcg
     3841 tgtaaaacgg caatttttcg cggaatgtca cccagatgag cggtccccaa gattgccagc
     3901 ggggagcgac aggaacagac cccacgcctt tatgaatccc gaactgaccc tgcccttcat
     3961 caagctcgct ctccctgtcc ctttactatc aattactcaa tcttgccgat tgccttgctg
     4021 acatccgccg gcgtttggta atcgtcttcc ggcagctgct caagaacatc cattacattc
     4081 tcatccgctc cttttgactt cgcatgatca acaatttcgt ccttgctcgc cgggtaatcc
     4141 atgccgccta aaaacttttg cacttgaatt gggttaactt ttgccatgac tcctcctgta
     4201 aggctggcga tcaggtttgg ctgagcgccg atttggcgcg caccaggaat tcctttggga
     4261 aacccttttt ccaaaatagg ccagatatcg gatgagtgca atgcgatatt cattaaattt
     4321 gtatgagcca gcgttcgcgt cacagaagct tcagcaaact cgatatctac cagtggcgct
     4381 tcgcgcgatt ccagcgataa agtgaccggg caaagatatt gtaggtcagg cgcatcaatt
     4441 gatgaaggaa gggcaaacgc aacaggtaag cgaaccatga ttggccaggt gtttgcgaca
     4501 tgaggttcag gaaagcgtcc gctcctacgt caaccctgcc cgaggcgttt ttgacatgca
     4561 gccgctcccg cacctcctcg agcggaacgc caagatcaga cacggcatca ggacgggtgt
     4621 gtatgtcgat ccactcgatg tccttgacac cgcatgcttc catgcggctt cgctgatcct
     4681 ttattccggc gttgcaaact gggcacgcac tgttgtaata gaccttcccg ggcatgtcag
     4741 cctccggctg tatgaaagat agaagcagca aacctgtcgg ctcctggtat tgattctcta
     4801 ctgttttctg cttgcatcaa tgtcgatgag taatgattgc agcttctcca ttccgacagg
     4861 tttggtaagg tgatagtcaa accccacact ttgtgctcgt cgttgatcct cttccgaccc
     4921 ccatccggtt aaagctacaa gaaccagccc gttcatgttg gaatcagaac ggagtcgttg
     4981 cgcaacttca tacccgctca tgcccgggag gccaatgtcg aggaatatga tgctgggttg
     5041 aaaggtacag gcagcctcca gcgtatctgg gccattgtga accgtcttta ccgtgtgtcc
     5101 aaacaacata aggagcattg caatggtctc ggagccatcc acattgtcgt ccactaccag
     5161 gatacgacga ggaacagata ggctacggga gccctcctct tctgcgactt gccttccgtt
     5221 gattacctgc actgccgcga gaggaagacg aacaacgaac gtactgcctt gcccgaggcc
     5281 tttgctttct gcggtaacct gtccaccatg catttcgaca agtcgcttga cgagggttaa
     5341 tccgatgccc aatcctccct gggatcggtc cagcgttttc ccgatctggc tgaataattc
     5401 aaagaccttt gggagtgcct ctggcgcaag cccgacccca ttatcccgca cccggatgat
     5461 cacgtcgttc tggtctctct cggcggtcag ctcgattcgt cctccttcgg gcgtatactt
     5521 cgcggcgtta ttaagaagat tgctgactac ctgagccatg cgggtcggat ctgcatcaag
     5581 caggagtccc tctttaggag gcgaaatgag caactggtgc cggctttcct caatgagcgg
     5641 gcggcttagt tccagtgctg tatccaatac tgctcgtaca tctacgcgct ctttcttgag
     5701 ctggaccttt ccgctggtga tgcgggaaac atcaagcaga tcatcgatga gacggaccat
     5761 atgctcgacc tgtcgctcca tgatctctcg tgtttttaca gtggcatcga tcgaggatgc
     5821 cagcttgagc acagcaaggc cagtgcgaac gggggcaaga ggatttctca gttcatgagc
     5881 cagtgtggcg aggaattcat ccttgcgtcg atcctgctcc cgcaactgct catagaggcg
     5941 ggcgttttcg atcccggcag aggcaatcgc agcaagctgt acgagaatcg tttcatcctc
     6001 ttcggtgaat tcgccttgag ccttgtccgt aagctgtacc agcccgaggt ttttccctcc
     6061 gtgaccgatc agcggtacgg cgagccatcc cctcacaggt gcatcatctt tggcaggttt
     6121 ttcaagccct ttccatgccg gatgcgcttc cagctcttgt tgagtcatcc gcatcggacg
     6181 attggcacga cgcacctcga catacatatc cgatatcccg ggcttctcca gccaggcgcg
     6241 gcagccggcg tactcgtccg acagcgacac tacgttaacc gccagagcct cgcgttgtga
     6301 aacagccaag gaagtgactg cctgatgagc gccaagcatg gaacgcgcct ctcctgtcaa
     6361 aattcgcgca atactgtccg tcgacagcgc cgcgttcatc gacttgcttg cattggctac
     6421 ctgtgccaga agcgcagcgt gtcgcttctc tgttgcgagg agagcagcca tctcttcttc
     6481 tgcttgtttg cggtcggtaa tatccagcac gtttcccgcg tagccgagga actggccgcg
     6541 ccgggaaaag cgggggcgcc cggcatcgat gacccaccgg tacacgccat cagcccgcct
     6601 cagccgatat tcgatggaga atggctcgtt ccgctcgttt gcgaagagga aatgttgcgt
     6661 tgctgcctcc cgatcctcgg gatgcacggc attgagccac ccgaaaccca tgccttcgtc
     6721 ctcgccctga ccggtgaatt cgtaccatcc ttgagaaagg aaagagcaga atccgtctgg
     6781 ctccgtcacc cagagcatgg ccggagcgac gtcggcaaaa ccgcggaagc gctcttcgcg
     6841 ttgttggaag gtctgcgagg cccgttgacc ctgcaagacg accaccgcct ggttggcagc
     6901 aaactccagc agtagccgtt cttgctcagt tgggaagttt gcgcgccggg aaccagcgac
     6961 caaaagatca tgctcttcaa ccaaacctag ccggatgacg gtcgttcgta gagtacctcc
     7021 gccaaacgga tcaggcattg tcgtaaccgg agccaaagtg cccccagcca ggagggaagc
     7081 gagtgaggct tgcatgtcag aaatatggct cgcggcatca gaacgatgct tgctacggat
     7141 agtctccgta aagccagcgt cgtcgcctgg ctggcaaagc cggatataaa ccaggtcgag
     7201 atcaagaata cttaagagaa cttgcgccag cttcccggcg atgtcctccg gtcccaggcc
     7261 agcccacacc gtgggcagag tcgaaagccc ttccagatct cgcattattc gccgcaagcg
     7321 gcacacttca tcactatcac tctcgccggc attcatagag ttttggcctt gagtcaccta
     7381 ggggtaagta gcatcggatc gtacctttgt ccaattgttc cggaacgtta aataactcat
     7441 agtgctctgc gctacctgtt ttctgctgag aaccgctgag acagccgggg gaggccatat
     7501 ggctgtaagt gctgcgttcc ccagggaagt cacttttgcc accgtcgaga gccacaacgt
     7561 gcatcagaaa aggaatttga tggattacga cattgacagc tttgcccaaa tgagcagaac
     7621 ggagaatatg aaatgaaaag aaattttgtg ctcatcattt gtattttatt tataacgccc
     7681 gtacacgcgg tgccactcga tacagccaac tggacgagct ggacgtcgtc gacaagcggc
     7741 agctttactc aggattccca cgttatcaac gtaacctata ccgggaatac ctttggaacg
     7801 aactacggtt cacacttcta caatgttcca acttcgttta cgaatagtga agtgacaaat
     7861 accccaggaa ccaatgggac gcttctgatg acgggcggta caagcgaaat aaacggattt
     7921 catttttccc agcccgtcgt cgatccactt atcagtcttt tcagcgtagg ccaggcaggt
     7981 gcccccgttt catttaattt cctgagcgat gtgaccttga gcatactgag tcaaggcgct
     8041 ggtaattggg ggggtggctt actcacccaa aacggcacct ctattaccgg atgggaaggc
     8101 aacgggctgc tcaagttcca tggaatctac accgatattt tatttacaac cccaaattat
     8161 gagtattatt acggggctac tgtcggggcg ctggccgata cccctgtgcg cccgacagct
     8221 atccctgagc cggcgaccta tgcattaatt ttgatgggcc tggctgtagg ctggacgttc
     8281 cgcaggaact aggcagacgt gatcctcgct tggacacgcg ggccacggac cccaccggga
     8341 caggaggcgt tgttgggcat caggagaaac tggggatgtt cactgtgggc gaactgaaac
     8401 tgaatagcgt ttgcagcctg tccagcacct gaggagctcg gccttgggca ggtagggcaa
     8461 ctgcgggggt tcgacctcgt tgttcgtaac catgaccccg actgcaagga tcggaaggag
     8521 ggccacatga gctcgacctc gcaggtctgc gacggttgct gcccgatgca atcggggtag
     8581 agcaaacgcc ggaggcgggg tgagcgcaag caatcgtccg gatcctgagt tgcgaaaact
     8641 tgctactcac agcgatagac gacccccagt acagtggtgt ttgtggtgcc caggctgccc
     8701 catgcgttcg tgttggccgt attttgaatc cagacggtat ttccgcccct ttccgccgct
     8761 tggttacgca gatcgttcct ggcacccagg agaagatttt tattcgaagt gtaatctccc
     8821 gtgaaccagt tgccctgaga cccgatcgca tcgccgagtt gcttgcagtt gccagtgggc
     8881 ttttcagtta ccagctcgac ggatctccct tcacgggtgg gagaaattgc cgaacatgcg
     8941 acgagaagag caggcagaag cgccaggatg ccgatcctca ttttcacgac cattattttt
     9001 ctcccactgt tcctcgtttc gccctcgttc caatggcatc cgctttcagt ctcgaggcaa
     9061 tcgatccagg aaaaaagatg atactccttc acgttcctcc cggaaaactg gaaagatgct
     9121 taagagaatt gtaaggttaa aaggaccaaa gtcaatcgcg gggaagtacc ttgccaagcc
     9181 tggttgacaa gctgccgata ctcacgaatg cccgccaata ctgaaattcg atgcccccca
     9241 tcccataccc tgttcatacg ctttccctgt atagcgccgg tattaaatag tcctatcttt
     9301 tgctgagcga aggggtttct cagttttttt tccgtcgtat ttcaggacac ttatttcgcc
     9361 gctgtcttcg agatatgcgg ccctgatctc attaaagtcg tcgatgcctt gttcacgcag
     9421 ctttgatttc aattccgtgg tagtaagcag ttcctgcctc atcgccttcc gttgtaactt
     9481 cccgtccttt ataaggagaa gcgggggggg ctcggccagc cggcggatca tcggaaatcg
     9541 gaaactcgcc cagtccaccg cgtaattcca ccctgcgata gtggagataa gaatgcaacc
     9601 atctgtgacc gagctataat tgcccgacat gccattgctg gcggcatctg caatcagcac
     9661 aaccatcatg acgtctgcaa tgccgatcgc gccgatatcc cggcgaagta tgaatctgaa
     9721 cagagcaaat ataaaccagt agatagccgt tccacgcacc accatctcgg cagggtttac
     9781 tgaaaagcca aagatatgag cccaatcgac catttgcgcg ctatgtattt gttccaatca
     9841 ttctactctt gctcccatta ctctctctat ccttgttttt gagtgccttg gcaatccttt
     9901 cgccgcttta tcaacccctt ccactgatgc tctcgcagag cttatcccga gggccgaaaa
     9961 cgtccagcgg cgccacttcc attcacagac ctaaaaggta tttacgcggt agcgatgggc
    10021 tgatctgtag gtcgcgttac actaaacttg cttctgcccg gccggacagc gatgctccta
    10081 tcaccggaca tggctctgtt cggcgcgttt tcttacggct tcaaacaatt cagggataga
    10141 aacggtgtgc gaccaattga ccgcaaaaga tgggatcaat ccgccggggt tcgtccgtac
    10201 cctgtaagtg aggcgagtgc gtttaccccc atccatcggc tcgacaatcc acgcaccttc
    10261 attgagcttg acgcgcacca ctcccgttac ctctgcctga gcctctgggg ccgcagtcca
    10321 ttccagcttg aaaactcccc cattcaacct ggaaccgcga ctcatcgtga catgaagcga
    10381 ataatcgcgt tcgcttacaa atgggggtgc gacacgcgca taggtgatca ggtcaaaatc
    10441 attgatgcgg cttagcacgc gggattcttg cacgtagggc atgaattcag ggtaatgctc
    10501 gaagtcccgg agaacatcga agatcatccc gggagccact tccagttccg aaaccgccag
    10561 gatttcatag gcgtgtccaa tatccttggt aaaaatgacg agatcgttct tgcgatacgc
    10621 ctcggtccag tctttaccgg ggctcgtcat gtcgagcgca tgcccggaag gagtgacgag
    10681 gaaaatggta acgaatgcag cggcaacgaa cgcagcggct ggggacatgg cggcgggcaa
    10741 cgcgagtcga gcacggaagc gactttgcca gctttccagt ccgcgtgttt gacgctggcc
    10801 atggattcgt cttgcatctg gcatcgctca gtctttcgcc aggcggatgc tgcggaacca
    10861 acctcctgca agaaccggca catctgcctc cactttcaac tctggagcgg ttgtaagcac
    10921 ccgctcaaaa acgacgtcga ggcgggtgac gagatgctga gatagcacgt tcagcgttcg
    10981 cttgaaccac gctgtttcac ccggcttcag aaatttgtcg aggatcagca cttttccgcc
    11041 gggcttcagc actcgcgccg tttccttcag acaggcgcgc gcatccggaa caatcgcgag
    11101 aatcagatgc aatacgacgt gatcgaagca ttcatcggca aatggcagcg ttgtgctgtc
    11161 accctggaca agatccagat caagcgttcc tatccttgcc tttgcgcgct gaagcatcgc
    11221 ctcagtaatg tccagccctg tatacctgtg ccctggcggc aaatagggaa tatcaaggcc
    11281 cgttccgata ccgctgataa ggatgttccc atttccttct ttcggcaatt gctccaggct
    11341 tgccgcgcgc agacccgttc ccgctgtggc cagcagcgca tcatagagcg gggcaataag
    11401 agtgtaggag cggcgcaggg acatctgggg cattgataaa gtacctttca gatatagcct
    11461 attttcggct ccgtttcttc cgttcaggcg gcgcacccgc attaaccggc tttcccggcc
    11521 cgcctgcaag agcttgccct tgaactggcc ccgggctctg gtgataagcc caatacaaca
    11581 tgagaattcc ggcaatcacc attggcagcg acaaccactg tcccatgctg ataccgagcg
    11641 tcattactcc catgaaaccc tcttccggct cgcgaaaaaa ttcggcaaac gagcggaaca
    11701 cgccatagcc gatcaggaac atcgccgaaa ctgctcccac gggcctgggt tttgatgaat
    11761 aaagccacag tagtacaaaa aatgcgactc cttccagtgc aaattcatag agctgtgaag
    11821 ggtggcgggg aactgcgtca acgttaggga agacaatcgc ccaagggaca tcagcagggc
    11881 ggccccataa ttctccatta atgaaattac caatgcgccc cgtccccagc cccagcggaa
    11941 ccaggggagc gataaaatcc gttacggcaa gccaccgcag tctatatttt cgggccacca
    12001 gcgtcattgc ggtaatgacg ccgagaaagc cgccgtgaaa cgacattcct ccctgccata
    12061 cggcaaaaat ctcgagcgga tgctgcacat aatatccgaa gtgataaaac agtacatgtc
    12121 cgagccggcc gcccactatg accccgagaa tcccgtaaaa cagcatatcg tcgagtgtgg
    12181 cgggcgtgaa agggcctttc ggattttgct tgatgcgata tcttccgagc aggatgaaca
    12241 gtgcgaatcc aaggaggtac atcaagccgt accagcggat ggcgagaggt cccagatgga
    12301 tggcaaccgg gtcgaactga gggtgaacaa gcatgggacc tcgggtttgg agtaaaatag
    12361 gtctgaatag gtgaataggt ctgatcgggg agcaccggat gaacggtacg tggttgctct
    12421 ccaggtgctg tagaccgcca ggcatagctg ggagaagtca agtacgctcc tggcaaccaa
    12481 ccggaatgca accaaccgga attatacata cggcaaagct gtcgcacagg gacagggaaa
    12541 acgcgttgcc ggtaaaaatg gatgcgtctt gcgattccca tcgatcttca tccaaactcg
    12601 atccctcttt gcttccacct catgccttca gtcatattaa ggagtattga tgccagataa
    12661 tcagcgcagc tgggccgtca cccagggtgc tcaacgttcc cccaaccgcg ccatgttgcg
    12721 cgcagtggga tttagcgata acgatttcct taaaccgatc gtgggcgtcg ccaacggata
    12781 ctcgaccatt acaccctgta acaaaggatt gaatgagctt gcgatcgctg ctgagtgcgc
    12841 actgaaagaa tcgggcgcga tgccgcaaat gttcggcacg atcacggttt cggacggcat
    12901 ttccatgggc accgagggaa tgaaatactc gctcgtgtcc cgcgaggtta ttgccgattc
    12961 gattgaaacc gcggttcagg cagagcgcat ggatggcgtg atcgccatcg gtggttgcga
    13021 caagaatatg ccgggcgcaa tgattgcaat ggcgcgaatg aacattcctg ccattttcgt
    13081 ttatgggggc acgatcaagc cgggccatta caaggggagg gatttgacga ttgtaagcgc
    13141 attcgaagcg gtcggccagt atacctctca caagatcgat gagaaggagt tgctcgaagt
    13201 ggagcggcac gcttgcccgg gagcaggttc gtgcggcggc atgtacaccg ccaacagcat
    13261 gtcctcggca tttgaggcaa tgggaatgag cctgccttat tcttccacca tggcggccga
    13321 agaccatgaa aaagtcgaga gtgccgcgcg gtccgccgaa gttctcgttg gggcaatcaa
    13381 gaaagacttg aagccgcgcg acatcatcac cagaaagtcc atcgagaatg cgattgcagt
    13441 gattatggca gtgggaggct caaccaacgc ggtgctgcat ttcctcgcga tcgcccacgc
    13501 ggccgaggtg gaatggacga tcgatgattt tgaacgcata aggggcaacg tgccggtact
    13561 ttgtgatctc aagccctcgg gacgttatgt tgcaaccgat ctgcataagg ccggcggcat
    13621 tccccaagtc atgaaaatat tgctggaaca ggggttgcta cacggagact gcatcaccat
    13681 tagcggtcag accatagctg aggtgctgga gaatgttccg tcaagaccac gggggaatca
    13741 ggatgtgatc cgccaatggg acaatccctt gtacgcccag ggccaccttg ccatactgaa
    13801 agggaacctg tctccggagg gttgcgtggc gaagattaca ggtgtcaaga caccgaaaat
    13861 aaccgggcct gcgcgagtgt tcgaatcgga ggaagcgtgc atggctgcaa tcctggcccg
    13921 ccagatccac ccgggggaag tggtggtgat acgttacgag ggcccgaaag gaggcccggg
    13981 gatgagggaa atgctctctc ccacctcgac tctgataggc gaggggcttg gcgactccgt
    14041 gggcctgata accgacggcc ggttttcagg gggaacatat gggatggtgg tcggacacgt
    14101 tgcgcctgaa gcgttcgtcg gcggcacgat cgcccttgtg caggaagggg actccatcac
    14161 tatcgatgcc gaaaagcgct tgctgcagct caatgtttcc gacgaagagc tggcgcggcg
    14221 ccgcgcgcaa tggaatccgc cccatccccg ttacaaccgg ggcgtgctcg cgaaatatgc
    14281 gaaactggta tccagcgcca gtactggcgc cgtcaccgat tgaacctgga ggagcgggag
    14341 tctccttgcg caagcaatga ctaaccacct cgcaggcgaa accagccctt atctgctcca
    14401 gcatgcggaa aacccggtcg actggtatcc ctggggtgaa gaggcactga cgctcgcgcg
    14461 aactcaggat aagccgatat tgctttccgt cgggtattcg gcttgtcact ggtgccatgt
    14521 catggcgcac gaatgctttg aagaccctaa agtcgcctca ttgatgaatc gggatttcat
    14581 caatatcaag gtggaccggg aagagcggcc agatatcgat cagatttacc aaacagcgct
    14641 ctatatgctc accggacgcg ccggcgggtg gccgctgaca atatttctga cacccgacca
    14701 aaagccgttt tttggcggta cctattttcc taaaacgtcg cgccacggat tgccgggtct
    14761 tctcgacctg ctgcctcacg tcgccgagac atactacgcg cgcaaggatg aaatcgagcg
    14821 gcagagcgtt tcaatgctgc aatcgttcgc cggcatgctt ccctccggta tagtcgaccc
    14881 ctcgggattt tccgggcaac ttctggaatt ggcgactaca gagctaaaga cccggtttga
    14941 ccccatcaat ggcgggttgg gtgctgcacc caagtttctt catcccactg agcttggatt
    15001 ctgcctgcgg cgttactttg acgcggacaa tcgggaaact ctgcatatgg cgacccatac
    15061 cctccagaag atggccgagg gtgggatata cgaccaattg ggcggcggat tttaccgcta
    15121 cagcaccgat cagtactggc atattccgca tttcgagaaa atgctgtacg acaacgggcc
    15181 gctgctccgt ctgtatgcgg acgcctggat tgcgacagaa aatcccttgt tcaagcgtat
    15241 cacagaggaa acggctggat gggtaatgcg cgagatgcag ggacaggtac aaaccgggga
    15301 ggctggaggc caaagaggtg aaggccaaaa agatgaaacc ccgagaggcg gttattactc
    15361 cagtctggat gcggattccg aaaacgagga aggcaagttt tatgtatggg ataggggcca
    15421 aatagcggca atactttccc cggaagaata tgcagtcgtg acaccctact atggactgga
    15481 gggtgaacct aatttcgaac acaagcattg gcatctggag atcgtccgac cgcttgccga
    15541 ggtagcggag gcaatcggta tcggcatcga ggaggcacaa aagaggcttg attcggcaag
    15601 aagaaaactt tttgtccagc gcgaattgcg catccatccg gggcgggacg aaaaggttct
    15661 gacaagctgg aacgggctca tggttagagg aatgtcccgt gccggacggg tttttggccg
    15721 cggggactgg atagagtccg cagcatccgc cgtcgatttc atccgctcat cgatgtggaa
    15781 aaataaccgc ttgctggcca ccagcaaaga tggaaaggcg catctaaatg cctatctcga
    15841 cgattacgcg ttcctgctcg acgggttgct ggaactgatg caagccgagt tccgcaaggc
    15901 tgatctggag tttgcgattg ttctggcgga cgtgctactt gaacaattcg aagatcccga
    15961 agcgggaggt tttttcttta ccagccacga ccacgagcaa ctgatacacc gcccgaagcc
    16021 agttcatgac aacgccatgc cgtcgggcaa cggcgtggct gcttatgcac tgcaacggct
    16081 cggccacctg ttgggcgaat cccgctattt ggaggccgcg gagcggagtt tgaagctgtt
    16141 ctattcctca ctgtcccgat attccagttc ctgctgcagt ctgctgggcg cactggagga
    16201 atggcttgaa cccccgcaaa cggtcatcct tcggggggag ggacgggcct tggctgaatg
    16261 gaccgacgca ttacgccaca tcgatccgtc gatactcgtg atcgccttgc cgtctgaact
    16321 tgcgggattg ccaccaagtt taagcaaacc ggcagcgacg caggatagtc ctgtcaacgc
    16381 ctgggtctgt cagggcgtta agtgcttgcc ggaaatttcc gacctgcacg aattgctgcg
    16441 cgtctgtaaa tttcaaggta agataagccc ctttctataa atcaacggat aaaggagtaa
    16501 gcatgaaagc tgtctggatg ggattggttg cagcatcaac cgtgttaatg gtgggtaccg
    16561 cgcaagcaaa cgcggatctg gcgaaagcca acggctgcac taactgtcat acggttgata
    16621 aaaaactggt cggccctgca ttcaaggatg tcgccgccaa gtataaagac gacgcaggtg
    16681 cccaagcccg tctggaagaa aaagtgaaga aaggaagcca gggagtctgg ggccccgttc
    16741 ccatgccccc gaacgcgcag gtcagcgacg ccaacgtcaa ggcactcgtg acatggatac
    16801 tgtcgctcaa ataaagaaga gcgattgaaa aaaaagccgg cacgtgccgg cttttttttt
    16861 aaacttttgc agtaatcact tatcggatgg tttgtgagtg gatacccgct taatgtcata
    16921 gccttgcttc tactgccgcc attcaacgtc attctgttgg ggacggcagg gattctgctt
    16981 ttgaaatggt ggcccaaaat gggttggttt ttgctggcag cctctctggc cctgctttat
    17041 gtgctatcaa gcccgttctt tgctgaacgc atgttgcaaa agctggaggt gccgcccgta
    17101 tccgttcccc tggacaatcg tatccaggcc atcgtggtgc tgggaagcag ctcatatctc
    17161 aacgctcctg aatatggcag tgataccgta acccgcttgg gcctggagcg cattcgctat
    17221 gctgcatggc tgcatcgaaa tactggcaag cctatactgg cttcaggcgg ttcccccttc
    17281 gcgggacgtt cgtctgaagc ctcgcagatg aaggccgcat tggaagagga gtttcgggtt
    17341 ccggtaagat ggactgaagg agcttcgggc aataccagag aaaatgccta caagagcttt
    17401 gcggttctga aaaaggaggg gattcgcaat atagcgctga ttacgcacgc atggcatatg
    17461 ccgagagcga tgcgtgaatt tgaacatgca ggattcgagg ttacccccgc gggcaccagc
    17521 tataccatcc ggcataaaac cgacttcctg gcgtttattc ctaccgcagc ggcattgcag
    17581 aaaagcagct ggttcttcca cgaaatcatc gggatggctt ggtacgggct gacggcagca
    17641 cgtgaacaac gctgaccggc aatccggagg cgttaatctc acacccatcg ttgcgcgcgt
    17701 tgttccggca aacaaagcta caatgacatt ggtacttcgg aaaggcgaaa aacatgaaag
    17761 catgtgtcag atggcaaggg ggagtgagct tcctggccga atcggggagc gggcatactt
    17821 tgctgatgga cggaccaccc gagtcaggcg gggcgaacca aggcccgcgg ccaatggagc
    17881 ttcttttgat gggaacggga ggctgcgccg cggtcgacgt cgtgctgatc ctgaaaaaag
    17941 gccgtcagga tatcagtaat tgtgttgtag agatcgaggc gaagcgcgct tcgcaagaac
    18001 ccaaggtttt cacccacatc cactttcact ttgttctaac tggcaggaaa ttgagcacga
    18061 cgcaaatcga gcgggcgatc aagctttctg ctgaaaaata ctgctctgcc tcgatcatgc
    18121 tgggtaaaac agcagagata acgcacgact atgaggtggt ggagatatag cgacccgcga
    18181 acaatcttaa acccagtagg cagtacgtgt catcaccgtt gatcccagct tcatcgcaac
    18241 cttcacagga agcggtagtt ctgcgcctcc atgatcaatg gcggtagtcg cgtggcccgc
    18301 ttcatcgatt tgcatttgcg ccacgatggc gcggctttta tgatcctggc gaggaagacg
    18361 cttgagatgg ccagccaggt gcgcttctac ctgtcgctcg gtttcagcaa cgaagccgag
    18421 gttccacttg tcgccgagca tcccggctac ggcaccaata gcgaatgatc cgccatacca
    18481 aagcggattc agcagacttt tgcggccgct caattccgcg atgcgccgct cggtccaggc
    18541 cagatgttcc gtttcctcgc gcgccgcttg ctccagactg tgctggacct tcgggtttcg
    18601 cgctgtaata gcttgccctt gataaagcgc ctgcgcgcag acttccccaa catggttgat
    18661 tcgcatcagc gcagttgatt cgcgtttctc cgaatcgctc agctcagcct cgggcaactc
    18721 cgcgcccggc acggggcgca gggtttgcgc cggagtcagc aaggtgcgca gcgccctgtc
    18781 aaaacctatg atgaatttgt ctatgttcgg cattcgatat ccgtaaatgg cttggacgtt
    18841 tttacatttt agcccattcg tattcgattt ttcgttttcc cctcttttta taccatttgc
    18901 cgcgccagca gcgtcacggg atgcacgact tcgatctcca tgccagcctg ccgtgccccg
    18961 cctgcaatat gcatcgcgca cccgacgttc gatgtagcga gatatcgcgc attgctggcc
    19021 atgagcgtag caagcttgct gtccaggagc ggctttgcca tttccggctg gtcaagaaaa
    19081 taggtaccgg cagcaccgca acattgatca tttccttcca gcggcacaac gcgggcatcc
    19141 gggatgcgcg cgagcagctt gtaggggtaa gaggaaccgc gcaggatgtt tcgcagggta
    19201 catggctcgt gtaccgcgat tttataaggc aatggcttta cccttacctc atcccatcct
    19261 tccgcctgtg cgaggaacgc gctgatgtcc gtgatcctgg cgataacgtt gcccgccccg
    19321 ttcttcgagc ttgaattgat gacgttaact tccctttctt caggcgcctc ccgccgttcg
    19381 cccgaaggtc gttttgaaga aacaggaaac tgctccgcca gatgcgcccc gcaacccgat
    19441 gctgtcgtta tgattgcctg taaatcgagg ctttcaaaag ccgccaagtt ctgatttacc
    19501 agattccggg cctcttccag ctcgccacca tgctgatata gcgcaccgca acatgtttgt
    19561 gcacttggca catgcactgt atatcccagg cggttcagca ctttgatggc cgcattgatc
    19621 gttggcgtgt cggtcacgcg cgcgatgcag ccaagaaaaa ggcccacctc gccgcgctgc
    19681 ttgcccttgg atggataaac ttcctgccac gtgcgttgcc ctccggcttc ccccctgtac
    19741 ggtttgtcaa tcgggcgtag ctgcgactcc agtaaggcca tcctggtact gcccagcagg
    19801 cggaagtgcc gaagccattt ttgcaagccg acagtttgat aaagccggaa aaacggtcgc
    19861 agtatctcga tgcgctccgg ctttgcgatc agttcacgca tgaggagttt tcgcagtccg
    19921 cgagaccatt taccggctgc cgcttcggga agctctatcc ctggcgaatc caggagcgcc
    19981 cgggttttat cgatcaattc tccaaaatgc acgccgctag ggcaggcggc ttcgcaggcc
    20041 cggcaggtca ggcatcgatc catgtgcaat gtgaaacggg cgtttacagg aatgcgcccg
    20101 ttcatcgctc cgcccatcag agcgatccgc ccgcgaggcg aatcggcttc ggattgggtg
    20161 atccggtagg ttgggcaatg cggcaagcaa aggccgcagg cgacgcaccg gtttgcttct
    20221 gcggctatct ctgctcgcag ctcatctgaa agtacgggtg ccgaaagctc gggcggaaga
    20281 tcaaccgggt tcatagggga tcgcggaaag ggagggtatg tccgcgttag agagaaagcg
    20341 ggccgaagag tttatttcag tgcgtttatt ttaaatggac gattaaaact ggcgggacgt
    20401 ttgctgactt gcttgaaaag cgtcccattg cagcaattgg ggagttgata gagccggtcg
    20461 cgctagccgc tctatatgta ctatgtgctc gccggaaaga gcggttaaat gagggatgga
    20521 gtaaggacac ggatcaaaac caagcgtccg cgccgcgggt tattcgggtc cgcgaattcc
    20581 gtatatgtag cgttccagct cgtgaatgag aaattcgcgt ccggaaatgg catcttttac
    20641 caggtcacca atcgaaataa tgccaataac attatcgttt tcaagtacgg gaagatggcg
    20701 tacgcgcatt tgggtcacca gggccatgca atcctccgcc aggtagtccg gattgacata
    20761 cgcaacctga gtagtcatca agttacgtac cgggatgtcc ctggcatgct tatccagctt
    20821 gtaggccttt cttgaatagt ctctttcagt gaagattccg gccagtttcc cgccttgcac
    20881 caccagcaac gcaccgatgt ttttatctgc catcaactgc attgcctcgt acaccgattt
    20941 gtcgggttca atgctggcaa cttcgtatcc cttgctttca agtaattgcc tgacagtcgc
    21001 cattgattcc tccctctcgc cttttccctt tcacccttgc ccggtttttg agttcccggt
    21061 ttcaaattgt tgcctttaat acctccgaag cctcatccaa tcatagcacg gcagtaaagc
    21121 aggcgccttc agacgggctc agtggattac agcatgtggc gcctgtcccc ttggctgaac
    21181 cctatcaaag attcccggat attctttcca aacgcaaccg ccttgaacag ctcgcccatt
    21241 tccgccgggc tcaccaattt ttgcaactgg tttgccaagg gaaggtaatc tttaacattt
    21301 tccaccgggg tttgccccag aagttcggtt atcccgcaat tgatgagaaa gtgcgcctgt
    21361 gtggcgtacc ccagcaagtc gaggccggag tcgagaccga ctctcgcaat agcgctgaaa
    21421 tcgacgtggc ttgtgacgtc ctgcaaacca ggaagataaa acggattatc gtgtacgcga
    21481 tgacgatagt ggcacattaa tgttccacta ttgcgctggg gatgatagta ctcacgctcc
    21541 ccgaaaccat aatcaatcag cagaatcacg cctgcctgga gaatatttcc caagctgtga
    21601 atgaaatgcc gcgcggaaag atttatttcg cttatatatt cctcatcagc cgacccggtt
    21661 cctgaaccgg ggtgaacggg caattcaagc gcagcagcaa aaagcgctcc gtcagcatgg
    21721 gtaagcggac gttcgctcca gttgaattca ttattttcga atgttacgcc gcgctcgaaa
    21781 agatttgcgc cccgccacac gaccagatgc accggcatcg cgtcaagcac ctcgttggcg
    21841 agcatcaatc cctcgaattt ttcaggcagg cgttcgagcc aggcgacccg ttccgaaagc
    21901 ttcggcgcgc atctttcaaa caattcccgc tgccgctgtt gtagatcgcc actgacctca
    21961 agaatgaagt atcggtttgg gaggctgccg agtttttcca gttcgagcag caagtcgagc
    22021 gccagtttgc cactgcccgc tccaaactcc aggatatcgc ccgcatcgct cccagtgagt
    22081 tcgaggattt gagcggcctg ctgcgccaag accctgccga acaggggaga catttcggga
    22141 gcggtgacga agtctccggc ttggccaaat ttcgtggccc ccccgctgta atacccgagg
    22201 cctggggcat acaaagccag ccgcatgtaa tgctcgaagg aaatccagcc actcgcagcg
    22261 ataatttccc tctgaatcag ttcgcttagc gcctggctgt gccgaagagc atcttgtccc
    22321 ggttcaggta acggcgatgt tggctgcaca gctactgtct accctgtact atatggattt
    22381 gcaatgcatt acgttttacc cgtagcgcgc tgttccgaag ggccaatcga gacgattgat
    22441 tgtcggccgg taaaagaaga gacgatttca ctgatagagg ggatcgctat ggagggcaaa
    22501 gtcgtacttg tcactggggg agcccggcgt gtaggcgcgg cgacctgcag aaggctgcat
    22561 cgactcggcg caaacctggt tgtccattat cgtgcttctg aacgggatgc caaaatattg
    22621 caggcggaac tcgagcgcga acgcccccgc tctgtggcgc ttgttcaggc ggacctgctg
    22681 gacactgcac acctgccgcg ccttgttcac gaaagcataa ggcattttgg aaggctggat
    22741 gcgctggtga acaacgcttc cagctttttc cctactccag tgggcgagat taccgagaca
    22801 gcctggaatg acctgatcgg cagcaatctc aaggccccgt ttttcctatc ccaggccgca
    22861 gcgccgcatc tcaagaaaca acacggctgt atcgtcaata tcgtcgatat ccattccgaa
    22921 tggcccctca agaattacgt ggtatacaac gcagcaaagg gcggcctggc ttcgctcacc
    22981 cggtcgctgg cgctggaact cgcgccggag gtccgagtaa acggcatctc acccggtccc
    23041 attctatggc ccgaggctgg ggagtggatg gatgaagcgg cacgccagca tatcatcaat
    23101 cggaccttgc tcaaacgcac gggcgaaccc gatgacatcg ctagaacagt ctcgtttctg
    23161 atagccgacg cgccttacat cacggggcaa atcatcgcgg tggatggtgg ccggagtgtt
    23221 aacctggagt gagggttctc ccgcatgcac taccggcctg aaatcttatg cggattgaat
    23281 gtttgatgct tgcttgccct tagggccttg tgtaacctca aacgttactt tttgaccttc
    23341 tttgagcgtt ttgaatccgg gcatgttgat tgcggagaag tgggcaaata aatcctctct
    23401 accatcatcg ggagtaatga aaccaaaacc ttttgagtca ttgaaccact ttactgtacc
    23461 aattgccatt tttatatcct ataaaaaacc cggtcgaaac ctggggaaac atgtcagtta
    23521 aaaaaaagcc aagaggcctc atttaggcct catttaaggc ctgacagcat tttgaccatc
    23581 ctcggaagag aaatatgttc ggtaccgtac ctacgaaatc catgtgtgag attgtcagca
    23641 gataccgctt ctgctgccag cgagtgaatc tgtttctggg gacgcacgag agataattct
    23701 ctggggagct cgatgttctc ccgtctccag cttgaacctg tgcgtacgtt cttcgtacca
    23761 gcctcgcaga gcttatagga cgtgctttac gaccggtcag aattgatgat tgagttgaac
    23821 ggaaacgatg tccgccgaat tgctgaaaga gccgcgaacg agcgggagcg atagcggatt
    23881 gcttgtcggc ctgtcaatct gggccttgtc gaaaaaaaca tggatatatc cgacatcaac
    23941 cgatgtccgc tgaccgatct tttgattaaa tccgaccgcc agcatcgttc ggtccgaatc
    24001 aggaagccgc accgtacgat ccattgcatt tcggatggga gactgatcat aggccacccc
    24061 cgctttcagg cgcagcctgt cgttatattg gtattgggtt ccaaaaccga cgcgaaagct
    24121 gtccttgaaa cccaatccct cgtttatcaa tgtcgcgcca tttatacggc tttgcaccgt
    24181 caatgccggg atgctgctcc agcctgtcca tgtaaaatca gccagaaaat gcaggcgatc
    24241 agtgaatatg tgggatatcg caacggatat ggtgtccggt aatttgatgg gagctgaggt
    24301 cggtatcttt cggcccaagc cctcaaagtg cccatcaaga tcaagcgtta tcgttgaccg
    24361 gtaggtgata cccacgcgag tctgaggcag tacctgaaac attgcgccca agttgtaacc
    24421 cacgccaaaa ccaccgccgg ttaccgttgt tcccgggtca atgggagtgg ggacgggggc
    24481 catgccgcgt agattcgcat caaatcttac gatatttaaa cctgccccca gagaaatgac
    24541 atcgttcatt cgccaggcaa tgctgggatt ggtattaact gtaatgattt gcgatgaagt
    24601 cccttgatag cgtcctataa aacttttgct ccagtcggtg atatccccaa atgttggcga
    24661 gacgcccagt ccaaggcgta gcgaaggatt gatggacatg ctccaataac cggcgggaac
    24721 aacaaaaacc ccgccagcct gccctccatt gtcgccaagc ggcccggcgg gaaatgcaat
    24781 actccccgta tctttaaacc ttagcgaggt catcaatatg ctggctgcac cggcaacatg
    24841 atgcccgggt cgaaggtagg tcattccagc ggggttaaag aacaccgtgg aagcatcttg
    24901 cgggacagct gccgcgccag catacgcata tccggccccc gccccattct gttctgtcaa
    24961 ggcgaatccg gaagcgtcgg cagtattgac cataaatatg ccgatcacaa aaaaaataag
    25021 cagagtggcc gatcgggaga agatcaaggc tgggtaaaag aactctccgg ctgatttctc
    25081 gccaatgaac tccttggcct ttttgtcgac tgtctcgttg atattgaaat cagaaaaaga
    25141 agccagataa ggatcgctat gcctcgtcat gacgattcac ttaccaaagt tttgtccaaa
    25201 gcaaaaatcg gcataaaccg gtcggctg
//
LOCUS       DASSHJ010000011        14298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1410808,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000011 DASSHJ010000000
VERSION     DASSHJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410808"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1056
                     /locus_tag="VFI43_00645"
     CDS             196..1056
                     /locus_tag="VFI43_00645"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET7060666.1"
                     /translation="MSFSHLLHDSPVFFISFCSFVGLAVGSFLNVVILRFPKMMERAW
                     VAQCSEFFGETVEPQPPYNIVTPRSACMHCGHKLKAIENIPLVSYLLLKGRCASCRAA
                     ISPRYPLVELLTAVISGLIAWRFGFGLTTFAALAFTWSLIVLAFIDIDIQLLPNDITI
                     PLLWSGLLVNLNETFTDIRSAVLGTVAGYAALWSIYWCFKLATRREGIGYGDFKLLAA
                     IGAWLGWQMLPLVVLVSSVAGTLVGVGLIMVAKRGRHAPIPFGPYLAVGGLIALFSGD
                     WLNSRYWNLI"
     gene            1060..1665
                     /gene="coaE"
                     /locus_tag="VFI43_00650"
     CDS             1060..1665
                     /gene="coaE"
                     /locus_tag="VFI43_00650"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646453.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7060667.1"
                     /translation="MRLIVGLTGGIGSGKTSAAKLFETLGADVVDTDVIAHELTQREG
                     AAIEAIRQAFGEKYVTDDAALNRESMRELVFSHEDARLRLEGILHPLIRAEVTQRIAD
                     FNGPYGIIVIPLLFEAGGYREIVRRVLVIDCSEDIQLARTTARKGMDERTARAIIAAQ
                     LPRLERIVKADDVIVNDLDMDHLRLEVEALHKKYRALTCTG"
     gene            1783..2538
                     /gene="zapD"
                     /locus_tag="VFI43_00655"
     CDS             1783..2538
                     /gene="zapD"
                     /locus_tag="VFI43_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HET7060668.1"
                     /translation="MICYEHPLNERIRTLLRLEDLFDRVAFFTARDDAVEHHAALVTL
                     FEILDVTSRADMKSDLLQELERQKHALEILRRKPGISEDALAQVLANIQAASHKLLNM
                     TGKIGEHMRENEWLMSIKQRAGIPGGACEFDLPSYHYWLHLDQDLRRSDLNEWFAPLL
                     PIEEGFRIVLHLLRNSGKTSQHTAYRGLFQQMGTGRVAHMLCLTLAEGFPCVPEISAN
                     KYALNIRFVIAGPRQKPKIWEEDVEFELTFCTL"
     gene            2613..3308
                     /gene="ispD"
                     /locus_tag="VFI43_00660"
     CDS             2613..3308
                     /gene="ispD"
                     /locus_tag="VFI43_00660"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7060669.1"
                     /translation="MTRFFALIPAAGTGSRMGNELPKQYLSLADRPMIYHALRRLCNS
                     SRIDRVLVVLARDDEEWRRHDWSDFSHKLTVVQCGGATRAESVINGLKAAREMLSIAD
                     EDWVLVHDAARPCLSESQLDRLMDDLAEDEVGGLLAVPVADTLKRSSADRRVGQTEPR
                     ENLWQAQTPQMFRHHLLMRALSMAGGTAVTDDAGAIEAMGLRPKLVLSDARNLKVTYP
                     QDLVLAELILGIP"
     gene            3446..3934
                     /gene="ispF"
                     /locus_tag="VFI43_00665"
     CDS             3446..3934
                     /gene="ispF"
                     /locus_tag="VFI43_00665"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7060670.1"
                     /translation="MTKFRIGQGFDVHQLVEGRKLIIGGVTIPHEKGLLGHSDADVLL
                     HAICDALLGAAGLGDIGRHFADTDHRYKNIDSRVLLRNVHALIEERDYEVVNIDATII
                     AQAPKMSPYIASMASNIAEDLRIAMTDINVKAKTAERLGPIGRGEGIEAEAVCLIVQS
                     DH"
     gene            3907..4503
                     /gene="thpR"
                     /locus_tag="VFI43_00670"
     CDS             3907..4503
                     /gene="thpR"
                     /locus_tag="VFI43_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7060671.1"
                     /translation="MPDSSERPLTQKIDRGKKNTLRLFFAIWPDSLGQEQMSGLTRRL
                     DLQSICCGRKTRAENIHLTLVFIGQVDASELEALLEAGHQTEELGIKAFDLVFDGIRY
                     WKHNRIVYVAVSALPPELMQLVNGLQFAVSDAGYQAEKRPYVPHVTLMKRASCAALPE
                     LPGPVIWQVREWMLVKSEQTSGGSVYSPVHRWSLGSPG"
     gene            complement(4510..4950)
                     /locus_tag="VFI43_00675"
     CDS             complement(4510..4950)
                     /locus_tag="VFI43_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HET7060672.1"
                     /translation="MSITWILVANASAAYLYANYGPKKGLRKLKEFRHEASREKGQDL
                     VSDRPGHNRSLGNGRGAYIPATDPKQHEARNFACQLARELEQGRITNSYQRLILVASA
                     PFIGLVNGNLGRNVRNLVSDTFEKDYTKTSEKYLTKHLETCIYL"
     gene            5094..5765
                     /gene="tsaB"
                     /locus_tag="VFI43_00680"
     CDS             5094..5765
                     /gene="tsaB"
                     /locus_tag="VFI43_00680"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7060673.1"
                     /translation="MKILALDTSTEYCSVALLSDNDVTSREDYAGQRHSELILPMVRQ
                     VLADSGIALTDLDGITFGSGPGSFTGLRIACGITQGLAFGADLPVVGICTLEALAQEA
                     GAEKVVTALDARMGEIYQAAYEKGPDEWQPVALPSLHRPENAPLLEGDNWTACGSGFD
                     VYEDRLRIRYEGCVKRIIRQMRPNACAIARLAVRKFVKGQSDDPSEAVPLYIRNKVAL
                     KENER"
     gene            5762..6223
                     /gene="rimI"
                     /locus_tag="VFI43_00685"
     CDS             5762..6223
                     /gene="rimI"
                     /locus_tag="VFI43_00685"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7060674.1"
                     /translation="MSARLNDMPTIRQMTQSDLDAVIAIEREVFLFPWTRGNFSDSVN
                     AGYHCLVLDQGGQLFGYGVMTVGADEAHLLTLSIAIESQRKGWGERLLRHFIWIARDL
                     NARSMFLDVRVSNVAAARLYERMGFKKVGRRRDYYPAMGGREDSVIMELRF"
     gene            6226..6990
                     /locus_tag="VFI43_00690"
     CDS             6226..6990
                     /locus_tag="VFI43_00690"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7060675.1"
                     /translation="MANEQRRKEILRELGLTPLWRAREAIPQERSAASAHAYPVEPQL
                     GAVRDGEEDGDEARRSRILRLEWDELEASVATCTACPLCEGRTRTVFGVGDKNADWLY
                     IGEGPGAQEDATGEPFVGQAGKLLDNMLAAIDLKRGHSVFIANIVKCRPPGNREPTHY
                     EARQCEPYLARQIDLIKPRLIIVLGKVAAQNLLGTDAGIGSLRGKLHQHAGIPVIVTY
                     HPAYLLRTPTAKAKAWEDLCFARSTMRKLIPLQYSD"
     gene            7032..7640
                     /locus_tag="VFI43_00695"
     CDS             7032..7640
                     /locus_tag="VFI43_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7060676.1"
                     /translation="MQKALLSLTLLLTFSLSGCGYNTLQTTDEQIKANWAEVVNQYQR
                     RADLVPNLVNVVKGFAAQEKDVLLGVTNARARVGSIQATPDLINDPEAFAKFQNAQGE
                     LSGALSRLLVVAENYPQLKSDANFRELQAQLEGTENRITVARNRYIKAVQEYNVTVRS
                     FPTNLTAMIFGYKVKPSFTVENEREISTAPKVDFSTPPPAAK"
     gene            7653..8537
                     /locus_tag="VFI43_00700"
     CDS             7653..8537
                     /locus_tag="VFI43_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HET7060677.1"
                     /translation="MARAGVKALAVAVLLLTACLAAADVAIPPLKSHVTDLTGTLSPA
                     EAAQLERKLADFEAKKGSQIAVLIVPTTQPETIEQYSIRVADAWKLGRKGVDDGALLL
                     VAKQDRTLRIEVGYGLEGVLPDAIAKRIIEETVVPRLRQGNFAAAINAGVDQMIGVIE
                     GEPLPPPKPRPGGRDASAETGGFLDHLIFIFIILLVAGRVLQSLFGRIIGATITGASG
                     GFLGWLLFSSVIAGAVVAVLGFLMGLIGTPGGFYRGGPGGWYGGGYGGRGGGGGFGGG
                     GFSGGGGGFGGGGASGRW"
     gene            8538..9044
                     /locus_tag="VFI43_00705"
     CDS             8538..9044
                     /locus_tag="VFI43_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET7060678.1"
                     /translation="MDLARIMRHFAIGHMAIRRTFPAAALTAIEKAIQKSETGHEGEI
                     RFAVEGALGTLSLLKGQTGRERAVEVFSDLRVWDTEENNGVLIYLLLADRNVEIVADR
                     GINTRVGNDEWESICRKMENAFTGGEFEAGVIAGIEAIGEHLRKHFPEKREHGENELP
                     DSPVILGE"
     gene            complement(9114..10388)
                     /gene="lplT"
                     /locus_tag="VFI43_00710"
     CDS             complement(9114..10388)
                     /gene="lplT"
                     /locus_tag="VFI43_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HET7060679.1"
                     /translation="MKRGFYTIMAAQFFSSLADNALLVVAIALLIELHAPAYLTPMLK
                     FVFVLFYVILAPFVGAFADSMAKGRVMLISNTIKILGCMLLFLASQQYFALAAYAVVG
                     LGAAAYSPAKYGILTELLPPEKLVIANGWIEGLTVASIVLGTVLGGLLISPAVSAVFL
                     SFDFPAIDTGVDTVPEAGILLIAIFYTIAAIFNLYIPNTGVDHRILKKNPVFLVHEFS
                     HCMKLLWTDKLGQISLAVTTLFWGAGATLQFIVLKWADAALGYPLSKAAQLQGVVAIG
                     IAIGAVIAGRFVSLRQSVKVIPLGIAMGIVVMSMTLARDLWVALPLLVLIGGLAGFFV
                     VPMNALLQHRGHILMGAGHSIAVQNFNENLSILTMLGLYAILISFDVHVYTVIILFGL
                     FVAVIMALIRQWHLRNQSIEDSLHLIGAHKGH"
     gene            10526..11605
                     /gene="alr"
                     /locus_tag="VFI43_00715"
     CDS             10526..11605
                     /gene="alr"
                     /locus_tag="VFI43_00715"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7060680.1"
                     /translation="MSRPVQALIDLSALEHNLSVVRRRAPRSRVMAVIKADAYGHGLL
                     RAAEALSRADGFAMLELDAAVRLRESGYRQKMLLLEGFFSMEELALIEQYNLSTVIHN
                     REQLDMLCAYNTRRRFKPDVFLKVNTGMNRLGFMPDHCSPVLEKIEANRAAGNITLMT
                     HFARADESDCDESIWEQLKHFTAIAAGRRFSCSLANSAAILRYPETHADWVRPGIMLY
                     GASPLWGTSAAELDLRPAMTISSKVIAVQTLKPGEGIGYGHIFRADRPMRVGVVAGGY
                     ADGYPRHAPTGAPVLVNNRRTRIVGRISMDMLHVDLSGLTDAGVGSPVTLWGKGLPVE
                     EVAQAAGTVSYELLCALAPRMRIVT"
     gene            complement(11853..12656)
                     /locus_tag="VFI43_00720"
     CDS             complement(11853..12656)
                     /locus_tag="VFI43_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7060681.1"
                     /translation="MHFKQIAPLMALCVSGLVAATTVTAQTQSTSTMAKVNGVAIPQS
                     RLEFIMKARAAQGQPDTPEARKALRDDLIAEEVIAQDAIKKGLDKDPDFAAQLDMARQ
                     SALVRAYQIDYVKNHPVSDEELRKEYEALKTQMGDKEYKAHHILVGSEAEAKDIIAKI
                     KKGAKFDKIAQEKSLDAGSKNKGGELDWAPAASYVQPFAEALTKLSKGQLTEQPVQSP
                     FGWHVIRLDDVRPLKIPPFDEVKNTLAQRVLQRQFAAYVMDLRSKAKID"
     gene            complement(12669..12950)
                     /locus_tag="VFI43_00725"
     CDS             complement(12669..12950)
                     /locus_tag="VFI43_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET7060682.1"
                     /translation="MTTVELITQKLAALEPQRIKIMDESARHSGHAGAKGGGGHYILT
                     IVSPKFAGRSQLERHRLVYDALQEMMHKDIHALGVKAYTPEEFDLILTH"
     gene            complement(12955..13263)
                     /locus_tag="VFI43_00730"
     CDS             complement(12955..13263)
                     /locus_tag="VFI43_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7060683.1"
                     /translation="MLYVINGEDVPDSLEKRMPARSAHLARIKALQEEGRLILAGPYP
                     AIDSLDPGPAGFSGSLIVAEFDSLEAAHEWARSDPYVAAGVYADLTVKPFKKVLPDNS
                     "
     gene            complement(13263..13808)
                     /locus_tag="VFI43_00735"
     CDS             complement(13263..13808)
                     /locus_tag="VFI43_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HET7060684.1"
                     /translation="MKFFFDLFPVILFFVTFKTYGIFAATAVAIGATFGQIGWVWFKH
                     GKVDTMLWVSLVIIVIFGSATLILQDEMFIKWKPSVLYWLFAVTLLLAQTIFKKNLIR
                     VMMKEQITLPDHVWTRVNASWATFFALMGAANLYVAFNYSTETWVNFKLFGFMGLMLA
                     FVVLQGLMLARYMEDKEDKET"
     gene            complement(13904..14296)
                     /gene="msrB"
                     /locus_tag="VFI43_00740"
     CDS             complement(13904..14296)
                     /gene="msrB"
                     /locus_tag="VFI43_00740"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7060685.1"
                     /translation="MKAEQDWKKQLTPQQYSVCREKGTEPAFTGEYWNCHENWVYVCA
                     CCGSPLFDSSAKFDSGTGWPSFSQPVNPANIASEQDNGFGMRRVEVLCSRCDAHLGHV
                     FEDGPFPTGLRYCINSASLKLEREHKKK"
BASE COUNT         3248 a         3648 c         4084 g         3318 t
ORIGIN      
        1 tgccggctat cctatggaaa tcattcagac ccaggaccgg gttgttaaag tggttcgcat
       61 tttttctatc ttgccccccg cgcacgaaat ggtagagaac tcggaaagtg acctgcccac
      121 agcctagtaa atcatgcttg atacaagcgc ataggaatta cggtttttta gaggccgatc
      181 gaatggctgg atcagatgtc ttttagccat ctgttgcatg attctccggt ttttttcatt
      241 tccttctgct cattcgttgg ccttgccgtt ggaagcttcc tgaacgttgt catcctgcgg
      301 tttccaaaaa tgatggagcg tgcgtgggtc gcgcaatgtt ctgaattctt tggggaaact
      361 gtcgagcctc agccgccgta taatatcgtt acgcctcgtt ccgcatgcat gcattgcgga
      421 cataagctga aggccataga gaatattcct ttggtgagct acctgttatt aaaaggccgc
      481 tgcgcctcct gtcgcgcagc aatttccccc cgctatcctc tggtagagtt gctcaccgcg
      541 gttatcagcg ggctcatagc ctggcgcttc ggatttggct tgactacatt tgccgcactg
      601 gcgttcactt ggtcgctcat tgttctggcg ttcatcgata tcgatatcca gttgcttccc
      661 aacgatatta cgatcccgct tttatggtcg ggattattgg taaacctgaa tgaaactttc
      721 accgatatcc gttcggcggt actgggaact gttgcgggat acgcggcatt gtggtccata
      781 tactggtgct tcaagctcgc cacgaggaga gagggcatcg gctacggcga cttcaaactg
      841 ctcgcggcga tcggcgcgtg gctgggttgg caaatgcttc cgctggtggt tcttgtgtca
      901 tctgtcgccg ggacccttgt cggggtgggt cttataatgg tcgccaaacg gggacgccat
      961 gcgcctattc catttggtcc ctatctcgcg gtcggcggcc tgatcgcgct attctcggga
     1021 gattggctta acagcaggta ttggaacctt atttaaccaa tgcgactgat cgtcgggtta
     1081 acggggggaa tcggcagcgg gaaaaccagc gcggcgaaac tgttcgagac tcttggggcg
     1141 gatgtggtgg acaccgatgt cattgcccac gagcttaccc agcgggaagg cgcagcgatc
     1201 gaggccattc gccaggcttt tggcgaaaaa tatgtaaccg atgatgccgc tctgaaccgt
     1261 gaaagcatgc gtgagctcgt tttctcccac gaggatgcgc gcctccgact tgaaggcata
     1321 ctccatccgc tcatcagggc tgaggtcacc caaaggattg cagatttcaa cgggccttac
     1381 ggaatcatag ttatcccttt gttgttcgag gcaggcggtt atcgcgaaat agtccgacgc
     1441 gttttggtca tcgattgcag cgaagacatt cagcttgccc ggaccactgc tcgcaaggga
     1501 atggatgagc gaactgcacg agccatcatt gcggcacagc ttccccgcct ggagcgaata
     1561 gtcaaggccg atgatgtgat cgtgaatgat cttgatatgg atcatctgag actggaagtg
     1621 gaagcgttgc acaagaagta ccgggcgctt acgtgcaccg gctgattccc gctggcgctt
     1681 gtcacttccg ccgtgatttt aattgtagaa ttccgttttg tagggcaata tcgcacttgc
     1741 tgttctgttg cttttcgttc agttcgatcc tgatccataa ccgtgatttg ctacgagcat
     1801 ccccttaatg aacgtattcg caccctgctg cggcttgagg atctgtttga cagggtcgct
     1861 ttctttaccg cgagagacga tgcagtcgag caccatgccg cgcttgtaac tctgttcgaa
     1921 attctcgacg taacaagccg ggcggacatg aaatcggact tgctgcagga actcgagcgt
     1981 cagaagcacg ccctggagat cctgaggagg aagccaggca tttccgaaga tgcgttggcc
     2041 caggtactcg ccaatatcca ggcggcatct cacaaactgc tgaacatgac cggcaagatc
     2101 ggcgagcata tgcgcgagaa tgaatggctc atgagcatca agcagcgcgc cggcatacct
     2161 ggaggcgcct gcgaattcga cctcccctcc tatcactatt ggcttcatct cgatcaagat
     2221 ctgcgccgca gcgatctcaa cgagtggttc gcgccactat tgcccatcga agaaggtttc
     2281 cgcatcgtcc tgcatctact gcgaaacagc gggaagactt cacagcatac cgcttaccgc
     2341 ggtctctttc agcagatggg aactggacga gtcgcgcata tgttgtgtct gactctggcg
     2401 gaaggctttc cctgtgtgcc ggaaatcagc gcgaacaagt acgcgctcaa cattcggttt
     2461 gttattgccg gcccccgtca gaaacccaag atatgggaag aggacgtgga gtttgagctg
     2521 actttttgca ccttatagaa ttcaggcaat cctcggtaaa ctcacccgat tacaatcttg
     2581 tccggtattc cacagttagg cagagcatcg caatgacgcg tttttttgca ctgattcccg
     2641 ctgcgggcac gggttcacgc atgggaaacg agctgccaaa acagtattta tcgttggcgg
     2701 accgcccgat gatttaccat gccttgcgca gattgtgcaa ttcctcgcga attgaccgag
     2761 tactggttgt gctcgctcgt gatgacgagg agtggcgaag gcacgactgg tcggattttt
     2821 cgcataagct caccgtcgtc cagtgtgggg gtgcgacacg tgcggaaagc gtgatcaacg
     2881 gcctgaaggc ggcgagggaa atgttatcga ttgcggatga ggattgggtg ctggtgcatg
     2941 atgccgcacg gccttgtttg agcgagagcc agctcgatag actgatggac gatctggccg
     3001 aggatgaggt aggtggactg cttgccgttc ccgttgcaga taccctgaaa cgcagttcgg
     3061 cggacaggcg tgtaggacag accgaaccca gggaaaacct gtggcaggca cagacgccgc
     3121 agatgtttcg tcatcacctg cttatgaggg cgctgagcat ggcgggtgga acagccgtga
     3181 ctgatgatgc aggcgcgatt gaggcaatgg gcctgcgccc aaaactcgtt ctgagcgacg
     3241 cccgcaacct gaaagtgacc tatcctcagg atctagtgct cgcggaattg atactcggaa
     3301 ttccatgagc ttctatatca agatgagctt ctgtagcgct tttcgctcgc cgcgggcgaa
     3361 acgacgcagt tgctgtacac cctgggctga tgcggacgac ggtctcattt gagaacgaaa
     3421 ttcaagaacg aaatttgaga aaacgatgac gaagtttaga atcggtcaag ggttcgacgt
     3481 gcatcaactg gtcgagggcc ggaagctcat catagggggt gtgaccattc cacatgaaaa
     3541 aggacttctc ggccactcgg atgcggacgt attactgcat gcaatatgcg atgcgttgct
     3601 gggtgcggca gggttgggcg acataggaag acattttgcg gatactgatc acaggtacaa
     3661 gaatatcgac agccgcgtac tgcttcgcaa cgttcatgcc ttgatcgaag agcgcgacta
     3721 cgaggtggtg aacattgacg cgaccatcat tgcccaggcg cctaaaatgt caccgtacat
     3781 tgcttccatg gccagcaata ttgccgagga tctgcggata gccatgacgg atatcaacgt
     3841 caaggcgaag acagccgagc gcctcgggcc gatcggccga ggcgagggca ttgaggcaga
     3901 ggcggtgtgc ctgatagttc agagcgacca ctgacacaaa aaattgaccg aggcaaaaaa
     3961 aacacgcttc ggctcttttt tgccatttgg ccggacagcc tcgggcagga gcagatgagt
     4021 ggactaaccc ggcgactgga tttgcagtcg atttgctgcg gaaggaaaac gcgagcagaa
     4081 aatattcatc tcactctcgt gtttatcggg caggtggacg caagcgagct ggaagcactg
     4141 ctggaagcag gccatcaaac tgaagagctt ggcataaagg cgtttgacct ggtttttgac
     4201 ggtatccgct actggaagca taaccggatc gtgtacgtcg cagtgtcagc cttgccgcct
     4261 gagctcatgc aacttgtcaa cggtcttcaa tttgcggtgt ccgatgctgg gtatcaggcc
     4321 gagaaaaggc cctatgtacc gcatgttacg ctaatgaaac gtgcgtcgtg cgccgctttg
     4381 ccagaattgc cgggtccggt gatctggcag gtaagagaat ggatgcttgt aaaatcggaa
     4441 cagaccagcg gcgggtccgt ttatagtcct gttcaccgct ggtcattggg ttcccccgga
     4501 tgaggcggct taaaggtaaa tgcaggtttc gagatgtttc gtcaggtatt tttcactcgt
     4561 tttggtatag tctttttcga aggtgtcgct cacaagattt cgtacgttgc gtcccaggtt
     4621 accattgaca aggccaataa agggagctga tgccaccagg ataagtcgct ggtagctgtt
     4681 ggttattctg ccttgctcca gttctctcgc caactggcac gcaaaattcc gcgcttcatg
     4741 ctgttttgga tcggttgcag ggatataagc gccccggcca ttgcctaagc tccgattgtg
     4801 gccgggccgg tcggaaacaa ggtcctgtcc cttttcccgg ctggcctcat gtctgaattc
     4861 cttgagtttc cttaatcctt ttttcggacc gtagtttgcg tagaggtagg ctgcacttgc
     4921 atttgcgacg agaatccatg tgatgctcat gactcctccg ttcaatgatg ttaaagtgaa
     4981 acgaggttta cgtcctgctc agcaacgaca ttgctcaacg gtggacattg gagttctatc
     5041 agagttttcc tctggaggca acctctaaat tatacgaatc cttcgccagt ttcttgaaga
     5101 tcctcgctct cgatacatct actgaatact gttccgtcgc cctattgtcg gataatgacg
     5161 tgacgagccg ggaggattac gcgggacagc ggcactccga actgatcttg cccatggtga
     5221 ggcaagtgct tgcagattcc gggattgcgc tcactgacct tgacgggatc acttttggtt
     5281 ccggccccgg atccttcacg ggcttgcgca ttgcatgcgg cattacgcaa ggactggcct
     5341 ttggcgcaga tttgcctgta gtgggcatat gcactctgga agcattggcg caggaagccg
     5401 gagcggaaaa agtcgtgacg gccttggacg cccgtatggg tgagatttat caggctgcct
     5461 atgagaaagg gcccgatgaa tggcagcctg tcgctctacc ttcgttgcat cgccccgaaa
     5521 atgccccgct gctggagggc gataactgga ctgcctgtgg aagcggtttc gatgtgtatg
     5581 aggaccggtt gcgcattcgc tacgagggat gcgtcaaacg catcatcagg caaatgcggc
     5641 cgaatgcgtg tgccatcgcg cggttagcag taaggaagtt tgtcaaaggg caaagcgatg
     5701 atccctcgga ggcagtacct ctttatattc gcaacaaggt tgctctgaag gaaaatgagc
     5761 gatgagcgcg cgcctgaacg atatgccgac aatccggcaa atgacccagt cggacctgga
     5821 cgcggtgatt gcaatcgaac gcgaggtttt cctctttccc tggacgcgcg gcaatttcag
     5881 cgattctgtc aatgccggct accactgcct cgtgctggat cagggggggc agcttttcgg
     5941 ttatggcgtg atgactgtcg gagcggatga agcgcatttg ctgacgttga gcatagcgat
     6001 cgagtcgcaa cgcaaggggt ggggcgagcg attgctgcgg catttcatat ggattgccag
     6061 agacctgaat gcgcgctcga tgtttctcga cgtacgtgtt tccaacgtcg ccgcggcccg
     6121 gctatatgaa cgcatggggt tcaagaaggt gggcaggcgc agagactatt accctgcgat
     6181 gggggggcgt gaggattccg tgataatgga actgaggttt tgaggttggc taacgagcag
     6241 aggcgtaaag aaatattaag ggagctcggc ctgacgcccc tgtggcgggc gagggaagcg
     6301 ataccgcaag aacgctcggc ggcatccgct cacgcatatc cagttgagcc tcaactggga
     6361 gctgtacgtg acggggaaga ggatggggac gaagcgcgac gatcaagaat tcttcgcctg
     6421 gaatgggacg agctcgaggc gagcgtcgct acctgcaccg cgtgcccgtt gtgcgagggg
     6481 cggacgagaa cggtttttgg agtaggagac aaaaacgcgg attggttgta catcggagaa
     6541 ggaccgggag cacaggagga tgcaacaggc gagccctttg tcggacaagc cggcaagctc
     6601 ctggataaca tgcttgctgc aatcgacctg aagcgcggcc acagcgtctt tattgcaaat
     6661 atcgtaaaat gccgcccgcc ggggaatcgg gaacccactc actacgaggc ccggcagtgc
     6721 gagccgtatc ttgctcggca aattgatctg attaagccta gactgattat tgtgctgggg
     6781 aaagttgcgg cacaaaatct gcttggtacc gacgcaggca tcggcagcct gcgtggtaaa
     6841 ctgcatcaac atgcgggaat tccggttatc gtaacctatc acccggccta tctgctgcgc
     6901 acgccgaccg ccaaagcgaa ggcttgggag gatctgtgtt tcgcccgcag cacgatgcgg
     6961 aagctgattc ccttgcaata ttccgattaa gccccacata cgcttggcgt attaataaga
     7021 ggagggaaga tatgcaaaaa gcgctcttgt ccctgacgtt gctcctgact tttagcttga
     7081 gcggctgcgg ctataacacg ctccagacga ctgacgagca aatcaaggcg aactgggctg
     7141 aggttgtgaa tcagtatcag cgtcgtgcgg acctcgttcc caacctggta aacgtggtca
     7201 agggtttcgc cgcccaggaa aaggacgtgc ttctcggagt caccaacgca cgcgcacggg
     7261 taggcagcat ccaggctacc cctgacctta tcaacgaccc cgaagcattt gcaaaattcc
     7321 agaacgccca gggtgaatta tccggcgctt tgtcccgctt gcttgtcgtt gccgagaatt
     7381 accctcagct caaatctgac gcgaacttcc gggagttgca ggcgcagctt gaaggcaccg
     7441 agaaccggat tacggtagcc cgcaatcgct acatcaaagc agtacaggaa tataacgtga
     7501 cggtccggtc ctttcccacc aacctcacgg caatgatatt cggttacaaa gtcaagccga
     7561 gctttactgt tgaaaatgag agggaaatat caactgcgcc caaggtggat ttcagcacgc
     7621 ccccacctgc ggccaaataa gccgtttcat ttatggctcg ggccggggtt aaggcgctag
     7681 cagtcgctgt actgctcctc accgcctgcc tcgccgcggc ggatgttgcg attccgcctc
     7741 tcaagtctca tgtcaccgat ctgaccggta cgctttcccc agccgaggct gcccaattgg
     7801 agcggaagct ggcggatttc gaagcaaaga agggcagtca gatcgcagtg ttgatcgtgc
     7861 cgaccactca acccgaaacc atcgaacaat attcgatacg ggttgcggac gcgtggaagc
     7921 tgggacgaaa gggcgtggat gacggcgctc tgctgctcgt tgcgaagcag gatcgaaccc
     7981 tgcgtatcga agtgggttac ggcctggaag gcgtgttgcc ggacgccata gcaaagcgca
     8041 ttatcgagga aacggtcgtt ccaaggctga gacaggggaa ctttgccgct gcaattaatg
     8101 cgggcgttga tcaaatgatc ggggtgatag aaggagagcc tcttccgcca cccaagccaa
     8161 ggccaggggg ccgtgacgct tccgcggaga cgggcggctt cctggaccat ctcatcttca
     8221 tcttcatcat cctgctggtg gcggggagag tcctgcagtc cttgttcgga cgcatcatcg
     8281 gcgcaacaat cacgggggcg tcaggcggat ttttaggctg gcttcttttc tcttcggtta
     8341 ttgcgggggc ggttgtcgcg gtacttggct tccttatggg cttgattgga actcccggag
     8401 gcttctatcg cggcggcccg ggcggctggt atggcggcgg ttatggcggc agaggaggag
     8461 ggggtggttt tggcggcgga ggcttcagtg gaggcggcgg cgggtttggg ggcggtggtg
     8521 cgtccgggag atggtgaatg gatcttgcgc gaataatgcg tcatttcgcg atcggacaca
     8581 tggctataag gcggacgttt cccgctgctg cccttaccgc gatcgagaaa gctattcaaa
     8641 aatcggaaac cggacatgaa ggcgaaatcc gttttgcggt agagggcgca ctgggtacat
     8701 tatccctcct gaaaggtcaa acgggacggg agcgcgccgt ggaagtcttt tcagatcttc
     8761 gcgtctggga tacggaggag aacaacggcg tgctgattta cctgctgctt gcggatcgta
     8821 atgttgaaat cgttgcggac cgcgggatca acaccagggt cggaaacgat gaatgggaaa
     8881 gcatctgccg taaaatggaa aatgccttta ccggaggtga gtttgaagcc ggagtcatcg
     8941 cgggaattga agcaataggc gaacatctac ggaaacattt tcccgagaag cgcgagcatg
     9001 gggaaaatga gttgccggat agcccggtca ttctgggaga ataagggaat ttatctgacc
     9061 gataccttag cgaaaggcag gcctggaagg agcgactttc agtattttca gtattaatgc
     9121 cccttgtgag ctccaatcag atgaagcgag tcttcaatgc tctgattgcg cagatgccac
     9181 tgtcgaataa gcgccatgat gacggccacg aacaggccga agaggatgat gacggtataa
     9241 acatgcacat cgaagcttat cagtatcgcg tacaatccca gcatcgtcag aatactcaag
     9301 ttctcgttga aattctgcac tgcgatggaa tgccccgccc ccataagaat gtgtccgcgg
     9361 tgctgcagaa gcgcattcat gggcactacg aaaaatcctg caaggccgcc gattaacacc
     9421 aaaaggggga gcgcgaccca caaatcgcgt gccagagtca tcgacatgac tacaatgccc
     9481 atggcgatac cgagaggaat caccttaacc gattgacgca atgacacgaa acgacctgca
     9541 attaccgctc ctattgcaat tccaatcgcc actacccctt gcaactgcgc ggctttgctc
     9601 aacggataac ccagtgcggc gtcagcccat ttcaacacga taaattgcaa ggtggccccg
     9661 gcgccccaga aaagcgttgt aacggccagc gatatctgtc cgagcttgtc tgtccacagt
     9721 agtttcatgc agtggctgaa ctcgtggacc aggaataccg ggtttttttt aagtatcctg
     9781 tggtccaccc ccgtattggg aatataaaga ttaaaaatgg ctgcaattgt ataaaatatc
     9841 gcaatcagga ggattccagc ttccggcacc gtatccactc ccgtatcaat tgcggggaaa
     9901 tcaaaggaga gaaacaccgc ggagactgcg ggcgaaatca gcagtccgcc aagcacggta
     9961 cccagtacaa tcgatgcgac agtcaggcct tcaatccatc cattcgcgat aaccagtttc
    10021 tcgggtggca gcagttcggt aagaatgccg tatttggctg gggaataggc cgctgctccc
    10081 agtccgacaa cggcataggc ggcaagcgca aaatactgtt gtgaggcaag gaagagcagc
    10141 atgcagccca ggatttttat cgtattgctg atcagcatga cccgcccctt ggccattgaa
    10201 tccgcgaatg ctccgacgaa cggcgcaaga attacgtaaa atagaacaaa tacgaacttg
    10261 agcatcggag tgagataagc gggggcatgc agttctatca ggagcgcaat tgcgaccacc
    10321 agcagcgcgt tgtctgccag cgaagaaaaa aactgcgctg ccatgatggt ataaaatccg
    10381 cgtttcaagc tatctcccct ccaaaaaccg tcggttgtgc aacccgaatt atacaatgcc
    10441 catccgtacc tttacgaagg catgtttccc tgaaatgtcc tggtttcgtc cctgaaaaaa
    10501 ctgaatttca ccgaagaagc tgcaaatgtc gcgtcctgtc caggcgctca ttgatctttc
    10561 cgcgctcgaa cataatttat ccgtagttcg ccggcgcgcg ccccgctccc gagtcatggc
    10621 ggttatcaag gccgatgctt acggtcatgg tttattgcgc gccgccgagg cgcttagccg
    10681 ggcggacggt tttgcgatgc tcgaactcga tgcggcggtt cggcttcggg agtcgggtta
    10741 tcggcaaaaa atgttattgc tcgaaggctt tttctccatg gaagagctgg cgttgatcga
    10801 acagtataat ttaagtaccg ttattcacaa ccgcgagcag ctggatatgt tatgtgccta
    10861 taacacgcgc cgccggttca agccggatgt atttctaaaa gtgaatacag gtatgaatag
    10921 acttggattc atgcccgatc actgttctcc cgttcttgag aagatcgaag caaatcgtgc
    10981 tgccgggaat ataacgctta tgactcactt tgcccgcgcg gacgaatccg attgcgacga
    11041 aagcatctgg gaacagttga agcacttcac ggcaatcgct gcaggtcgtc gtttctcttg
    11101 ttcattggcc aattccgccg ccattctgcg ttatccggaa acacatgcgg actgggtgcg
    11161 gcccggcatc atgctatatg gcgcatctcc cctctggggg acgagtgcag ccgagcttga
    11221 tttacgccct gcgatgacga tatcgagcaa agtcattgcg gtacaaacgc tgaagcccgg
    11281 tgaaggaatt ggttatggtc atatctttcg cgccgacagg ccaatgcggg tcggcgtagt
    11341 tgcgggcggt tacgcggacg gatatccgcg gcatgcccct accggcgcgc ctgtattagt
    11401 caataacagg cgtacccgta ttgttggccg catatcgatg gatatgctgc atgtggatct
    11461 aagcgggctg acggatgcag gggtaggcag tccggtaacg ctttggggaa aaggcctgcc
    11521 agtggaagag gtcgcacagg cagctggtac ggtcagctac gagctgctgt gtgcgcttgc
    11581 gccaaggatg cggattgtca cctgatccgg ttaagtcaag tcgtgcactc tcgttgtacc
    11641 cagaagggag ccaagccgct gatcaatcaa gaagcaggaa gctgagctaa gcgggagggg
    11701 tagtgggtct gaccaggaga taataaagga aaggataata aagaaacaaa ggacaacaag
    11761 gggataaagg acaacaaagg gctggggctg cgtccggagg tgctttccgg gcaacggcct
    11821 gcccggaaag ttaaatactg gcagtcggac gattaatcta tttttgcctt ggaacgaaga
    11881 tccatcacgt acgcggcaaa ctgcctttgc agcacgcgtt gcgcaagggt attctttact
    11941 tcatcgaatg gtggaatttt gagaggccgg acatcgtcca ggcggatcac atgccagccg
    12001 aaagggctct gtacaggctg ctccgtaagc tggcctttgc tcagtttcgt cagggcttcc
    12061 gcgaagggct gaacatagct cgcggctggg gcccagtcga gctcaccccc cttgttcttg
    12121 ctgcccgcat ccaatgattt ttcctgggcg attttgtcaa attttgcgcc tttcttgatt
    12181 tttgcaatga tgtctttcgc ttcagcctcg cttccgacga ggatgtggtg ggccttgtat
    12241 tccttgtccc ccatctgggt tttcagtgct tcatactcct tgcggagttc ctcatcgcta
    12301 accggatggt ttttcacgta gtcgatctgg tatgccctga cgagagcaga ttgccgggcc
    12361 atgtcgagct gagcggcaaa atcaggatcc ttgtcgagtc cttttttaat tgcgtcctga
    12421 gcgatgactt cctctgctat aagatcgtct cgcaacgcct tgcgagcttc cggcgtgtcg
    12481 ggttgtcctt gcgccgcgcg tgccttcatg atgaattcga ggcgagattg cgggattgcc
    12541 acgccgttca ccttggccat ggttgacgtc gattgggtct gcgcggtcac ggttgttgcg
    12601 gcaaccagtc cggaaacaca cagcgccatg agcggcgcga tttgtttaaa atgcatggaa
    12661 cttccttgtc agtgggtcag aataagatca aattcttccg gagtatacgc ctttacacct
    12721 agcgcgtgaa tatccttatg catcatctcc tgaagcgcgt catatacgag ccgatgtctt
    12781 tccagttgcg atctgcctgc aaattttgga gacacgatag tcaggatata gtgaccaccg
    12841 ccgccctttg cccccgcatg tccggaatgg cgggcgcttt cgtccattat cttgatccgc
    12901 tgcggctcaa gggcagcgag tttttgcgta atcaattcga cagtggtcat cgaatcatga
    12961 attatcagga aggactttct tgaatggttt taccgtcagg tccgcataaa cgccggcagc
    13021 gacgtacgga tcggagcggg cccactcatg cgccgcttcg agagagtcga attcggcgac
    13081 aatcaggctg ccggaaaaac ctgccggacc cgggtcaagg ctatcgattg cgggataagg
    13141 acctgcgagg ataaggcgcc cctcttcctg gagggccttt atccgcgcga gatgagcaga
    13201 tcgcgccggc attcgctttt ccaggctatc gggaacatcc tccccgttta tgacgtacag
    13261 catcaggttt ccttgtcctc tttgtcttcc atgtatctcg ccagcatcaa tccctgcagc
    13321 acaacaaagg cgagcatcaa ccccatgaac ccgaagagtt tgaaattgac ccaggtctca
    13381 gtggaatagt taaaggcgac ataaaggttt gcggcgccca tcagggcgaa gaaagtcgcc
    13441 cagcttgcat tcacgcgtgt ccacacatga tcgggcaggg tgatttgttc tttcatcatt
    13501 actcggatca agttcttctt gaatatcgtc tgcgccagaa gcagtgtcac ggcgaacagc
    13561 cagtagagca ccgaaggttt ccacttaatg aacatttcat cctgaagtat cagcgtggcg
    13621 cttccgaata tcacgatgat gacgaggctg acccagagca tggtatccac cttgccgtgc
    13681 ttgaaccata cccagccgat ctgcccgaaa gtggcgccaa tggcaaccgc ggtcgcggcg
    13741 aatatgccgt aggttttgaa cgtcacaaaa aacaggatta cgggaaacag atcaaagaag
    13801 aatttcataa gcgcttacgg aacagttcga gctttttgct ggctgggcaa ttatgaggct
    13861 tatcatggaa aaaggggaaa aagccagtat cttccctaaa gccttacttt tttttgtgtt
    13921 cccgctcaag cttgagtgag gcggagttga tgcaataacg caaacctgtg gggaaagggc
    13981 catcctcgaa cacgtgtccc aaatgtgcat cgcaccgcga gcacaaaact tccacacggc
    14041 gcatgccaaa gccgttgtcc tgttctgaag cgatattggc cggattgacc ggttgcgaaa
    14101 atgaaggcca tcctgtgccc gagtcgaact tggcactgga atcgaacagg ggagagccgc
    14161 agcaggcaca gacgtatacc cagttctcgt ggcagttcca gtattcgccg gtgaaagcgg
    14221 gttctgttcc cttttcccgg catacggagt actgctgcgg cgtgagttgt tttttccaat
    14281 cctgctcggc tttgatca
//
LOCUS       DASSHJ010000012        21884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_209486, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000012 DASSHJ010000000
VERSION     DASSHJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 21884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21884
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209486"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFI43_00745"
     CDS             complement(<1..213)
                     /locus_tag="VFI43_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060686.1"
                     /translation="MNHNPFNSLKDISLPEGRKASFYSLPALEAAGLGNVSRLPVSIR
                     IILESVVRNCDGNKITEGHVRRLAGWQ"
     gene            complement(309..1085)
                     /locus_tag="VFI43_00750"
     CDS             complement(309..1085)
                     /locus_tag="VFI43_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7060687.1"
                     /translation="MRITCDIEGKIAFVTGANRGIGKAIVDAFLNHGAAKVYAGVRRP
                     ASADHLIERYGAKVVPVQLDLGKPETITEAAGVAHDVQIVVNNAAVFKASTPLDPDAI
                     EALELGMHINVFGLIRMAQAFAPVLKQNGGGAFVQINSVASLVCASNFATHSACKAAS
                     YSITQALRNLLELQGTAVLSVHPGLIGSDMGKAAGLSGIPERPELVAEGIVDALRAGD
                     FHLFPDALARRVGQAYLGFSKTVVEPGIPEYRPEQGSTKA"
     gene            1770..1997
                     /locus_tag="VFI43_00755"
     CDS             1770..1997
                     /locus_tag="VFI43_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060688.1"
                     /translation="MAATRSYKIIFANNQGSFAVNGLALACDGSWRDAVQRYNGKHDL
                     AFIDVEEENAAHLEEMLEQDDNVLSYLCTDH"
     gene            complement(2154..2408)
                     /locus_tag="VFI43_00760"
     CDS             complement(2154..2408)
                     /locus_tag="VFI43_00760"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HET7060689.1"
                     /translation="MRFESSGWAEYSIEDFATSMDATEKQRSDFHDIKRRIIEPAVKE
                     AKKRIEMGRKIKNAPIQIGETQYQAVERERREYVQARAQA"
     gene            2817..3554
                     /locus_tag="VFI43_00765"
     CDS             2817..3554
                     /locus_tag="VFI43_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HET7060690.1"
                     /translation="MTDTRPQLALLHGFLGFVKCGPFEYFRGVREMLRRMDIDPLTPK
                     VPGDGTIAERAEALAHALFRTRAPAFALIGHSMGGLDARYLITHLDPDRRVKSLITVS
                     TPHHGTPLARWFLESPGPIEDYIRRIGRTGLGELTVEARAAMPIPDREDVDYSSYSGR
                     RPPAELPFWLHQYGRIIGEDNDGMVSVRSAQWGQFRGRMHADHIELIGWSVALPNRQT
                     GRPFNYLPFWARAANEALAVAKQKLQG"
     gene            3561..5012
                     /locus_tag="VFI43_00770"
     CDS             3561..5012
                     /locus_tag="VFI43_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060691.1"
                     /translation="MDQHEDKKPNMSWFDWLVFVLPSLLFITLGLESAFGSATDAQGH
                     PHRDFRAVAHWFDPVFFQYNLVMALLIVLVVPSLALSYIQSMASRKERRLYRDVPAER
                     RGEIRRRMGRRASFSSYRGSVWLTTIVVLLGVSILLLFKPVSSREEIGLDFSMGSNML
                     TMGPFTELFETNPSAYYSHLVRNMIAFEFGFLGAYVYFIGSVVRAYFTMDLTSHTFVD
                     GAIRMIVASVLALVLSFAFDFVAFGADSKVLSPHPASVASTANPTGSAQSSGSSHPND
                     ASAESTTSTDKSESASTQIPAGLSLLPVVAFFFGFYPKRASLAIERIVVKTMRNITPG
                     DSYRALPLSMLAGMSYSHELRLEREGFDNIENLSSADPVDLAIRTCFSYTQLKQWIDE
                     AWLAVHLREDYPEFVRRTGISTSEDLRCFLCNSGFANQDGIAQLVAALTSDPAVALSW
                     KVRLEALKMLLEANKAPSQKVVVPRPVDETKTD"
     gene            complement(5061..6512)
                     /locus_tag="VFI43_00775"
     CDS             complement(5061..6512)
                     /locus_tag="VFI43_00775"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HET7060692.1"
                     /translation="MFSSISNVPPQLDQVLVDIADYVVERQITSELAYETARYCLMDS
                     LGCAMEALTYSSCHRLLGPTVQGTTVLNGARVPGTSFQLDPVQAAFNLGTMIRWLDFN
                     DTWLAAEWGHPSDNLGGILAVADWVARNALAEGKEPLTMCDVLTAMIKAHEIQGGLAL
                     ENSFNKVGLDHVVLVKVASVAVVAHLLGANRGQIVDALSQAWVDGQALRTYRHAPNTG
                     SRKSWAAGDATSRAVWLALITLKGEMGYPAALTAKTWGFYDVLFKGRPFRFQVPIRQW
                     GSYVMENVLFKISFPAEFHAQTAAECAMQLHPWVRDRIDEISRITIRTHEAAIRIIDK
                     KGPLHNYADRDHCMQYIVAVSLIFGRLTAADYEDEIALDPRIDALREKMICVDEPQFT
                     RDYYDPSKRSIANALTVEFMNGEKLDEVVVEYPLGHRLRRSEGIPLLETKFRTNLARI
                     FPAEQQEGIFNLCFDARRLQSTPVDEFVDMFVI"
     gene            complement(6830..7312)
                     /locus_tag="VFI43_00780"
     CDS             complement(6830..7312)
                     /locus_tag="VFI43_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060693.1"
                     /translation="MTNGTAIQEVVIHRVEADVALGYLATEDWELARQSLIELEDFRS
                     TRSDLELEFDIDLANIEAIIDLPTDSFERLKQELTAALDAKLLLFSEAFRVKICVELD
                     YCNLKKGKRWRVVEYLIGALAIVFDKGLIPLAFYIFKREILDELCKCLPEQETGQIAG
                     "
     gene            complement(7372..8022)
                     /locus_tag="VFI43_00785"
     CDS             complement(7372..8022)
                     /locus_tag="VFI43_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060694.1"
                     /translation="MIQPVFPLIFIRTCVSLAALMFMSGAHAEKDSTDEWRLHRPDNH
                     ATQKESISGGACAVQTGAFPVGFNAYVVPEGDHPSYPPFCSPVPAGPLNLVIDILSQE
                     IREFPLAVKLMKLEDSGERKILSVPATAYTSGNIPLAVNLEPLAKYNVLLIGDGGGSN
                     NGNLVTIPIEVRRAGDFVHTGNSGMGIGFLIVVLALLGVVGGVMHFWKPASANRKT"
     gene            complement(8206..8898)
                     /gene="alkB"
                     /locus_tag="VFI43_00790"
     CDS             complement(8206..8898)
                     /gene="alkB"
                     /locus_tag="VFI43_00790"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HET7060695.1"
                     /translation="MIQFDNEPVTLALFQDEDAGQWLSEREELCPGAVVLRRLALPYQ
                     AEILDALQAVTAEAPFRRMSTPGGFRMSVALTNCGSCGWVSDRTGYRYDAIDPESGKP
                     WPRIPPVFLNLVQHAASLAGFENYRPDACLVNRYEPGARLSLHQDKDEHDLSSPIVSV
                     SLGIPAVFLWGGLRREHKTMRIPLGHGDVVAWGGPARLRYHGVLPVKEGYHSFAGRYR
                     INLTFRSVTLKG"
     gene            complement(9063..9950)
                     /locus_tag="VFI43_00795"
     CDS             complement(9063..9950)
                     /locus_tag="VFI43_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HET7060696.1"
                     /translation="MSFLRMLALLLVIIRTAFASDGVIERSHYLLNSFNVLSYNIYMR
                     PASLFADAQAERGAVLPSKLRGFDVIVFSEAFDNKVRDQLLADLAEEYPFRTSILGGD
                     HGFVQDGGVIVVSRWPITAETQRLFGDVCVGGDCWADKGVLYARFEKHAQTYHVFASH
                     AQAGREMGQRQTRMQQLGIIKSFVDDIGPSGTEPVFIAGDLNVDKYDAVEYAAMLRIL
                     DAWHPKPSGHPYTVDSAINKWAGYRLYLDYVLVSNRHLQPLDAIIETLIPRSRTLWGG
                     GYDLSDHFPVFGHFLFSSP"
     gene            complement(10030..11199)
                     /gene="prpC"
                     /locus_tag="VFI43_00800"
     CDS             complement(10030..11199)
                     /gene="prpC"
                     /locus_tag="VFI43_00800"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HET7060697.1"
                     /translation="MNQATDIGVLTSPPKAKKSVVLSGVVAGNTAISSVGRTGNDLHY
                     RGYDILDVAETCEFEEIAYLLIHEKLPTRVELDAYMEKLKTLRSLPAPLKIVLETIPA
                     TAHPMDVLRTGVSALGALLPEAEAHSLDRARDLADRLLACLGSMLLYWYHYSHHKRRI
                     ELETDDDSIGGHFLHLLHGKPPPPSWVRAMNTSLILYAEHEFNASTFTARVIASTGSD
                     FYSAITGAIGALRGAKHGGANEAAFEIQKRYQSGEEAEVDIRRRLAAKEIIIGFGHPV
                     YTLSDPRNRVVREVARKLSTEAGDMKMFEVAERLERVMWDAKNMFPNLDWYSAVSYHM
                     MGVPTPMFTPLFAIARTSGWAAHIIEQRIDNKIIRPSANYIGPEEREFVPVSERQ"
     gene            complement(11183..12091)
                     /gene="prpB"
                     /locus_tag="VFI43_00805"
     CDS             complement(11183..12091)
                     /gene="prpB"
                     /locus_tag="VFI43_00805"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HET7060698.1"
                     /translation="MTVSPGYRFREAIAQERPLQVVGCINAYAARLAELTGFKAIYLS
                     GGGVAAASLGMPDLGISTLDDVLTDVRRITDVTCLPLLVDADTGFGPSAFNIARTVRS
                     LIKAGAGAMHIEDQVQAKRCGHRPSKVLVGQDEMVDRIKAAVDARTDPGFAIMARTDA
                     LAGEGLQAAIERSRAYVEAGADMIFPEAVTELETYRKFSSAVNVPILANMTEFGVTPL
                     YTVEELADANVSLALYPLSAFRAMSAAALKTYQAIRGEGSQRSVIPAMQTRDELYEFL
                     DYHAYEDKLNELFSDPSIKPGNEPGN"
     gene            complement(12119..13108)
                     /locus_tag="VFI43_00810"
     CDS             complement(12119..13108)
                     /locus_tag="VFI43_00810"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7060699.1"
                     /translation="MNTPVRIAVTGAAGQIGYSLLFRIAAGDMLGRDQPVILQLLDIP
                     QLLPSLKGVLMELEDCAFPLLAGVTTTDDPKAAFKDAGIAILVGARPRSKGMERRDLL
                     EANGVIFATQGRALDEAARRDVKVLVVGNPANTNAYIAMKNARGLNPRNFSAMMRLDH
                     NRALWQVAAKLEKPVSSVRKMIVWGNHSSTQYPDLSHAEVGGDKVSDLIQDSGWIENH
                     FIPTVQNRGTEVIAARGLSSAASAANAAIEHVRDWVFGTREDDWTSMAIPSDGSYGIP
                     EGVMYGSPVVCRDGAYRLVSGLEISETDRKKMDITYQELVDERASVKHLLGEA"
     gene            13460..13837
                     /gene="sdhC"
                     /locus_tag="VFI43_00815"
     CDS             13460..13837
                     /gene="sdhC"
                     /locus_tag="VFI43_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET7060700.1"
                     /translation="MQKKRPKYLNLLEIRQPLPAIVSILHRISGALLFFPGIPLLLLA
                     LESLLSSPQTYAQFQSVFAHPLFKAAFVLAVWLFLHHLLAGIRFLGLDLHYAIEIHQA
                     RLTSKLVLAAGIVLTLLIGIAIW"
     gene            13831..14178
                     /gene="sdhD"
                     /locus_tag="VFI43_00820"
     CDS             13831..14178
                     /gene="sdhD"
                     /locus_tag="VFI43_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HET7060701.1"
                     /translation="MVRRVVTGAHYGLRDWLSQRVTALVMVVYVLCLVAGLLLSPPRD
                     YAGWKALFSNEWMRTASLLFLLSLFWHAWVGMRNILMDYVHATGLRLTLQVLVILSLV
                     FYAIWATEILWMS"
     gene            14207..15970
                     /gene="sdhA"
                     /locus_tag="VFI43_00825"
     CDS             14207..15970
                     /gene="sdhA"
                     /locus_tag="VFI43_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET7060702.1"
                     /translation="MAIGQRKFDVVIVGAGGAGMRAAILLSEAGLKVAVLSKVFPTRS
                     HTVAAQGGIAAALGNVTEDYWYWHMYDTVKGSDYLGDQDAIEVMCRQASEVVYELEHY
                     GMPFDRLENGKIYQRPFGGQSQNFGGAQATRSCAVADRTGHAMLHTLYQRNVRANTQF
                     YVEWMGLDLIRDEEGDVCGVTALDIETGEVMILQARATLFATGGAARIFSASTNAFIN
                     TGDGLGMAARAGVPLEDMEFWQFHPTGVHGVGVLISEAVRGEGGYLVNKNGERFMEHY
                     APHAKDLASRDVVSRALSTEIKEGRGCGKDADHLLLKLDHLGPEVITTRLPGIREIAI
                     KFANVDPIHDPIPVVPTAHYMMGGIPTNYYGQVVAPYKTSPEEVVQGFYAAGECACVS
                     VHGANRLGTNSLLDILVFGRAAANQIIEDLKKNSHHKPLPANAGDKALARLMRLDSQK
                     EGENVAQVGDAMRRTMQTHCGVFRFPDLLKEGVAMIEEIAERARNTVIGDKSRVFNTA
                     RVEALELDNLVEVAVATMVSAEARTESRGAHAREDFPFRDDNKWLKHTLYFAEDRRLD
                     YKPVRLKPLTVESFPPKPRTY"
     gene            16011..16706
                     /locus_tag="VFI43_00830"
     CDS             16011..16706
                     /locus_tag="VFI43_00830"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET7060703.1"
                     /translation="MKFSIYRYDPDKDDKPYMRDYDVALEPTDRMLLDALVRIKTMDD
                     SLSLRRSCREGVCGSDAMNINGRNGLACITPVKDLKEPVTLHPLPGLPVIRDLVVDMS
                     QFFKQYHSIKPYLINNDPPPETERLQSPAERAELNGLYECILCACCSTACPSFWWNPD
                     KFVGPAGLLQAYRFIADSRDQATAERLDYLEDPYRLFRCHSIMNCADVCPKGLNPTSA
                     IESIKDMMVKRTV"
     gene            16703..16933
                     /locus_tag="VFI43_00835"
     CDS             16703..16933
                     /locus_tag="VFI43_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HET7060704.1"
                     /translation="MKNQERARWRCRRGLLELDIVLQRFVDIHYEKLDEEGLRQFEAL
                     LGLADNDLWDMIAIKEAADNNVDPVLRLLRAV"
     gene            17005..18306
                     /gene="gltA"
                     /locus_tag="VFI43_00840"
     CDS             17005..18306
                     /gene="gltA"
                     /locus_tag="VFI43_00840"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7060705.1"
                     /translation="MEDNRTATLDLHNEAGPLEFPLLAGTMGPDVVDIRTLYSKAGVF
                     TYDPGFLSTASNSSAITFIDGDKGILLYRGYPIEQLAVQCDFLEVCYLLLNGELPTSE
                     QKAAFDHKIKDHTMVHEQLARFYTGFRRDAHPMAVMVGVVGALSAFYHDAMDIADASN
                     RELSAFRLIAKLPTITAMAYKYNIGHPFIYPRNSLSYAENFMHMMFATPAEEYKSNPI
                     LARALDRILILHADHEQNASTSTVRLAGSSGANPFACISAGIACLWGPAHGGANEACL
                     NMLQQIGNESRIGEYIERAKDKNDPFRLVGFGHRVYKNFDPRAKLMRETCYEVLDELG
                     LHDHPLFKLALKLEKIALEDEYFISRKLYPNVDFYSGIVQQALDIPPSMFTAIFAMAR
                     TAGWIAQWNEMISDPEHKIGRPRQLYVGATTRDVVPIGSRK"
     gene            18338..21160
                     /locus_tag="VFI43_00845"
     CDS             18338..21160
                     /locus_tag="VFI43_00845"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7060706.1"
                     /translation="MSQGLSTRSTDSVLFGGNAPFIEELYEEYRRDPYSVTPEWHAYF
                     DQLQQSGQAARSSSALSPQTPRRVTPATPAELDERALTMERKQIGVLQLINAYRFLGV
                     RHANLDPLGHQEKPHVPELDPAYYGLTDADMTTIFGTGSLVGAPRAPLGDIIQVLEKT
                     YCGSIGVEYMYISDTEQKRWIQNRIEGPRAQPDYSVEYKRHILERLTAAEGLEKYLHT
                     RYVGQKRFSGEGGESLIPLLDNLLQRAGQMGVQQLVIGMAHRGRLNVLVNTLGKVPAD
                     LFQEFEGKHPQELTEGDVKYHQGFSSAVSTPGGVMRVSLAFNPSHLEIVDPVVEGSVR
                     ARQHRLGDKEGDLVLPVLLHGDAAYSGQGVVMETLNLSQTRGFGTGGTVHVIINNQIG
                     FTTSDPRDARSTLYCTDVSKMIEAPIFHVNGDDPEAVLMVTEIALDFRVRFHKDVVVD
                     MVCFRTLGHNEQDEPMVTQPLMYKIIAKHSGTRELYAQKLEAEKVITPGDAERMVKSY
                     RDDMDAGRNPNKTVVYDYKAPDAVDWSPFLKNTPWDIPVRTGLPMDELKKLAECLTDI
                     PAKFKLHPRVEKIIADRRLMAEGKLPLDWGMAETLAYAALLKDGYGVRISGQDSGRGT
                     FFHRHAVLHDQDRERWNEGVYIPLRHIEPNQPDFTVIDSLLSEEAVLAFEYGYATAEP
                     DELEVWEAQFGDFANGAQVVIDQFIASGEAKWGRLCGLVMMLPHGYEGQGPEHSSARL
                     ERYLQLCAEYNIQVCVPSTPAQMFHVLRRQMIRPLRKPLILMTPKSLLRHKESVSSLE
                     DIADGSFQPVIPDMGNLDAGKVRRLIACSGKIYYDLAAYRREHKIEDVAIIRIEQLYP
                     FPHKDLQREVERYPNARDILWCQEEPGNQGAWHRIQHYLARHKRPDQVLGYALRPSSA
                     SPAVGYFAKHNDQQKKVVAAAFREKI"
     gene            21170..>21884
                     /locus_tag="VFI43_00850"
     CDS             21170..>21884
                     /locus_tag="VFI43_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7060707.1"
                     /translation="MMSVEVKVPLLSESVSEATLLKWHKKEGDYVDRDENLIDIETDK
                     VVLEVPAPAAGVLAKIIKGDGATVTSGEVIASIDTEPAKDTAVRGAQPSSQRETGTGQ
                     AARKQMSWPEPQSQRKPSPPPPAGAPAHMMPAARGAAAAAGLGAAEVGSIKGSGRGGR
                     ITKEDIVAHVEHKSPPAPPPVESGPPAVEPPPPPSDKQPTPAPAPSAKPISPPAPPAQ
                     PAGDRLERRESMSRLRARIA"
BASE COUNT         4976 a         6147 c         5940 g         4821 t
ORIGIN      
        1 ctgccatccc gccagccgcc tgacgtgccc ctcagtgatt ttatttccat cgcaattgcg
       61 cacgaccgat tccaggataa tgcggatgga aacaggcaag cgtgaaacgt ttcccaagcc
      121 agccgcttcc agcgccggca aagaataaaa actcgctttt cttccttccg gaagcgaaat
      181 gtctttaaga gaattgaatg ggttgtgatt catggaagtg ctcctgagaa tttcgctatc
      241 cgttattggg cgtttggcta agaactgttt atagggcata tgcgattatt tctcaaccaa
      301 agccaaattc atgctttggt cgaaccttgc tccggtcggt attccgggat tccaggttcg
      361 accactgttt tggaaaagcc aaggtatgcc tggcccactc gccttgcaag tgcatcgggg
      421 aaaagatgaa aatctcccgc tctcaaggca tccacgattc cctcggcaac aagctcaggc
      481 cgctcgggaa tcccggataa acctgctgcc ttgcccatat ccgacccgat aaggccgggg
      541 tgcacgctta gcacagctgt tccctgcaac tccagcaaat tccgcaatgc ctgggtgatt
      601 gaataagaag ccgccttgca agccgaatgg gtagcgaaat tggaagcgca aaccagtgat
      661 gcgacggagt tgatctgtac gaacgcgccg cccccgtttt gcttcagaac aggggcaaag
      721 gcctgcgcca tcctgatgag accgaatacg ttgatgtgca tccccagttc cagcgcctcg
      781 attgcatcag gatccagcgg tgtagaagcc ttgaatacag cagcattatt caccacgatc
      841 tggacatcgt gcgccacgcc tgcggcttct gtaatcgtct ccggcttgcc cagatcgagc
      901 tgtaccggaa cgaccttcgc cccatagcgc tcgatcaggt gatcggcgga agcgggacgt
      961 cgaacgcccg catacacttt cgccgctcca tggttcagaa atgcgtcaac tatcgccttt
     1021 ccaatgccgc ggttggcccc ggttacaaaa gcaatcttgc cctcgatgtc gcaggttatt
     1081 ctcattcctg ccgaagttct gcctgaggcc tatccattct gatgaaaccg tcgctcctgt
     1141 gcgttaataa ccgacacgtc ggtaaattat attcctggta aacggcccgt tctaacattg
     1201 gacggaaatt tcatgtttcc gctcgtgaac tgtgatcgca tcgggatcct ccgcttatga
     1261 aaatatccgc cccgtgttcc tattagacgg aacagaaaag aattttgctt atttttaagc
     1321 gtttttagct tctgcttgtg gatggataga gctgtctatc aggaatacat ccgtctacca
     1381 ggaactcgac ttttcagcaa gtatttaatt tatacaaata cgtaagcttt agcatcctaa
     1441 tactttaaaa cactgttaat acttacttaa aactcctgaa acgctactaa agatgccttc
     1501 acggtaaaag gtgtgaagac ttcgataccc gatccactgt ataggcatcc ttgcactcgg
     1561 ccggcacccg aactctacag tagaccggat aaggcttttc gattacgtga gtctggatca
     1621 cgggcttccc cgcacatcct tatagagcaa ggatagctat tagcaccagg attttgaact
     1681 ctttccctct tgttagcagg aacaatgttt ctgtagagtg tgaatcgcgc agcaaccgat
     1741 taacaagcca aatcaagagg agaaataaag tggcagcaac cagaagctac aaaatcatct
     1801 tcgccaacaa tcaggggtcg tttgcggtaa atgggctcgc ccttgcgtgc gacggctcgt
     1861 ggcgagatgc ggtacagcgc tacaacggta agcatgatct ggcgtttatc gacgtggaag
     1921 aagagaacgc ggctcacctg gaggaaatgc tggagcagga tgacaatgtt ctctcctact
     1981 tatgtacgga tcattaaaaa gagatgaccc tgacgaaatc ctcaaagccc ggcaattgct
     2041 cgggctgacg cagcccagtt tgggaaactc ctgcacgcca gaacgcaccg tgcgcatgtg
     2101 ggagagcggg caacgaaaca tggccccagc aaggcaagac ctgttccagc ccatcatgcc
     2161 tgagcgcggg cttgaacata ctctctccgc tcccgctcta ccgcctggta ctgagtctcg
     2221 ccaatctgta tcggggcatt cttgatcttg cggcccatct ctatccgctt cttcgcctct
     2281 ttcacagccg gttcgattat ccgacgcttg atgtcatgaa aatcgctccg ctgcttttct
     2341 gttgcatcca tggacgtggc aaaatcttcg atggagtatt ccgcccatcc cgaggattcg
     2401 aaccgcatca acagccaata gccgccatga gtaaattgaa cggaggatgg aagtctgttg
     2461 caactgataa ctcgtgaatt gatgctttaa tcccagaagt gcggaagcag ggggccacca
     2521 atgcaactca acctaagcct cgtgcttctc atagtcagct tggccgatcc atgccgcgac
     2581 cgtctgtgaa gacctggtcg atctcttttt cacttgtaca gatgcgaacg ataagcgaca
     2641 gggaacgagg acggcgcatg ttatacgcat aacagcggct caaattcgcg gatttcatat
     2701 attattggaa gatcaccatc ggaatctgag gacgcgactt ccgcaagtat ctgttccgca
     2761 cgttattcct tgcctccggc tgctgttgtt ttcgtcactt ttattgattc tcgatcttga
     2821 ccgatactcg cccgcaactt gcgctattgc acggctttct cggcttcgtg aagtgcggcc
     2881 ccttcgaata tttccgtggc gtcagagaga tgctgcgccg gatggatatt gatccgctca
     2941 cgccaaaagt cccgggcgat gggacgattg ccgagcgcgc agaggcgctg gcccacgcgc
     3001 tcttccgcac gcgcgcgcct gcatttgcct tgattgggca cagcatggga ggcttggacg
     3061 cacgatatct gatcactcat ctcgaccccg atcgccgtgt aaaaagcctg ataaccgttt
     3121 caacgccaca tcacgggacg ccgctggcac gttggtttct tgagtccccc gggccgatcg
     3181 aagattatat caggcgcatc ggcaggactg gcctgggtga attgactgtt gaggcgcgag
     3241 cagcgatgcc gattcccgac cgcgaggacg tggattattc ttcttactcg ggcaggcgcc
     3301 cccctgcgga gcttcccttc tggcttcacc aatatggtcg catcattggt gaggataatg
     3361 acgggatggt ctcggttcga tcagctcaat ggggacaatt tcgcggacgg atgcacgcag
     3421 atcatatcga gcttatcgga tggagcgttg ctttgcccaa ccgccaaaca ggacgtccat
     3481 tcaattatct tcccttctgg gcgcgcgcgg caaacgaggc tctcgcagtc gcaaagcaaa
     3541 aattgcaggg ttagaagaac atggatcagc acgaggataa aaagcccaat atgtcctggt
     3601 tcgactggct ggtgttcgtg ctgccatcgc ttctctttat caccctcggg ttggaatccg
     3661 cgtttggctc agcaaccgac gcgcagggac acccgcatcg ggacttccgt gctgtcgcac
     3721 actggttcga tccagtgttc ttccagtaca acctcgtgat ggccttgcta atcgtactcg
     3781 tggtgccgtc acttgccctg agctatatac agtcgatggc gagccgaaag gaacggcgcc
     3841 tgtatcggga cgttcccgcc gaacgccgcg gagaaatccg caggaggatg ggtcggcgcg
     3901 cgtccttcag tagctatcgg ggcagcgtct ggctgacgac cattgtggtt ctgctgggcg
     3961 tctccatcct gctgctcttc aaaccagttt catctcgtga ggaaataggc ctggacttca
     4021 gcatgggctc gaatatgctg accatgggac cctttaccga gctgttcgag accaatccca
     4081 gtgcctatta ctcgcatctc gtacgtaaca tgattgcttt cgagttcggc ttcctcgggg
     4141 cttatgtcta ctttattggc tcagttgtgc gcgcgtactt caccatggat cttacttcgc
     4201 ataccttcgt ggatggcgcg attcgcatga ttgttgccag cgttctggcc ttggtcctgt
     4261 ctttcgcttt cgattttgtt gcctttggcg ccgattccaa agttctgtca cctcatccag
     4321 caagtgttgc ttccaccgca aacccaaccg gttccgcaca atcctcgggg tcttcgcacc
     4381 cgaatgacgc ctcggccgaa agcaccacat ccacagacaa atcggagagt gcaagcacac
     4441 agataccagc gggcctcagc ctccttcctg tggttgcttt tttctttggc ttttatccca
     4501 agcgggcgtc gctggccatc gagcggatcg ttgtcaaaac aatgaggaac attactcccg
     4561 gcgacagcta ccgagccctg ccgctctcga tgctggcggg catgagttat tcgcatgagt
     4621 taaggctgga acgcgagggg ttcgacaata ttgagaactt gagcagcgcc gatcccgtgg
     4681 atctcgctat tcgcacctgt ttcagttata cccagttgaa gcaatggatc gacgaagcct
     4741 ggcttgcagt ccatctacgt gaggattatc cggagtttgt ccgccggaca ggcatttcca
     4801 cgagcgaaga cttacggtgc tttttatgta actccggttt cgctaatcaa gatgggatcg
     4861 cgcagcttgt ggccgcgctg acttcggatc cagcggtcgc cttatcgtgg aaggtaaggc
     4921 tggaagcact caaaatgctg ctcgaggcaa acaaagcccc ttcgcagaag gttgtggtgc
     4981 ctcgccctgt ggatgagacc aaaacggact gaaatctgtg ccgattcatc cttaacggat
     5041 ggcaacaacg ccggttaagt tcagatcacg aacatatcga caaactcgtc gacaggagtt
     5101 gattgcagcc gcctggcgtc aaagcagaga ttgaatatcc cttcctgctg ctcagccgga
     5161 aaaatacggg caaggtttgt tctgaattta gtctccagta acggaatccc ttcgcttcgc
     5221 cggagcctgt gtccaagggg atactccacc acaacttcat cgagtttttc tccattcatg
     5281 aactccactg tcagagcatt ggcaatggag cgcttcgaag gatcgtaata gtctcgggta
     5341 aactgcggct cgtcaacgca gatcatcttt tccctcaatg catctattcg cgggtcaaga
     5401 gcaatctcat cctcgtaatc tgcggcggta agccgtccga atataaggga cactgcaacg
     5461 atatactgca tgcagtgatc gcggtcggcg tagttgtgca gtgggccttt cttatcgatg
     5521 atgcggatgg cggcctcatg cgttcgaatg gttatcctgc tgatttcatc gatccggtcc
     5581 ctcacccagg gatgcagttg catcgcgcac tccgccgccg tctgggcgtg gaactcggcg
     5641 ggaaacgata tcttgaacag gacgttctcc attacgtatg aaccccactg cctgatcggt
     5701 acctggaacc ggaacggtcg ccccttgaac agcacatcat agaaacccca ggtcttggcg
     5761 gtgagggcgg cgggataccc catctccccc ttgagcgtga tgagggccag ccacacggcc
     5821 cggctcgtgg catcccctgc agcccaggat tttcgcgaac ctgtattggg agcgtggcga
     5881 taggtccgca gcgcttgccc gtccacccag gcttgcgaca gggcgtcgac gatctggccc
     5941 cggttggcac ccagcagatg cgcgaccact gcaactgacg caaccttcac cagaacaaca
     6001 tgatccagtc caaccttgtt gaaactgttt tccagcgcca ggccgccctg gatttcatgg
     6061 gccttgatca tggccgtcag cacatcacac attgtcaacg gctcttttcc ttccgctagc
     6121 gcattgcgag cgacccagtc cgcaaccgca aggataccgc ccagattatc ggaagggtgc
     6181 ccccattccg cagccagcca ggtatcgttg aaatccagcc agcggatcat ggtgccgaga
     6241 ttgaaggcag cctgcacagg gtcaagctga aacgatgtac cgggaacccg ggcgccattg
     6301 agaacagttg tcccctgaac agtcgggcca agcagacgat ggcaggagga gtaagtaagc
     6361 gcttccattg cgcagcccag cgaatccatg aggcaatagc gggcggtttc gtatgccagt
     6421 tcgcttgtta tttgtcgctc gacgacgtag tcggcaatgt cgacaaggac ctgatcgagc
     6481 tgggggggga cgttggaaat ggatgaaaac aaaagaggtg aggaagtggt agagatatta
     6541 gcgccgcgta ttggctcgta gcccaaaaac ctcgtcagat cctctctccg atttaatggg
     6601 agagagcggg agagagggaa accaaggcgg ggtcaagcag cttagcttat aagggcaata
     6661 attcgcttta gtcaaacaaa atcttggaga ttccaggcaa ggaaaagact gaccagcttt
     6721 acaaaattta ttcctttctg cagacttatt gaaaacattt atgaagtact tctaccgacc
     6781 ggcgcaatgc attgcacaga ttgcggtact ggtggttcgg cgtcccgatt caacctgcaa
     6841 tttgccccgt ttcctgttca ggcaaacatt tgcacaactc atccagtatc tcgcgcttga
     6901 agatataaaa agccaggggg atgaggccct tgtcgaaaac aatggccaag gcgccgatca
     6961 ggtattcgac aacgcgccat cttttgccct ttttcaggtt acagtaatcg agttccacac
     7021 agattttgac tcgaaacgcc tcgctgaata gtagaagttt cgcatccaga gcagccgtga
     7081 gttcctgttt caacctttcg aaagaatccg taggcaagtc gatgatcgcc tctatattcg
     7141 caaggtcgat gtcgaattca agctccaggt cacttcttgt acttctaaaa tcttccagtt
     7201 cgatgaggct ttggcgggcg agctcccaat cttcagtggc aagatagccc aaggcgacat
     7261 cggcctcaac gcggtggatg accacctcct gaatggctgt accgttagtc attttctctt
     7321 ttcataaaat acgtagccac tgacgcgttt acgcgcaccc cggctgacct actatgtttt
     7381 ccggttagca gaagcgggtt tccagaaatg cattacaccg cccacaactc ctaacagtgc
     7441 cagtaccact atcaggaacc ctatgcccat gccgctatta cccgtatgaa caaaatcccc
     7501 tgcccgcctg acttcaatcg ggattgtaac cagattaccg ttattgctgc ccccgccgtc
     7561 gccgatcagc aacacgttat atttggcgag cggctcgaga ttgacagcca gggggatgtt
     7621 tcctgaagtg tatgcagtag ccggtacgga taatatcttg cgttctccgg aatcttccag
     7681 cttcatcagt ttcacggcga ggggaaactc gcggatttct tgcgacagaa tatcgattac
     7741 aagattaaga ggtcccgctg gcacgggaga acagaacgga ggataggaag gatggtcgcc
     7801 ctcgggcacg acgtaggcat tgaaaccgac cggaaaggct ccggtctgga cagcacaggc
     7861 gcctcccgag atcgactctt tttgtgtcgc atgattatcg gggcgatgca ggcgccattc
     7921 gtccgtcgag tctttctcag cgtgggcgcc tgacataaac atcagtgccg ccagtgacac
     7981 gcatgttcgg ataaaaatca gcgggaagac gggttggatc atgccgggtt tcctgttctg
     8041 gatatagcag gtatgttaca gcgaatttta gtcgtaaaac gttactgaga cgcgcctata
     8101 aacatataac aacccgctat gcacgctttc attcttgcgt ggatacaggt tcttgctttt
     8161 acattcttag gttcgcgcgg gagatcgagc agacatatgt aagcgtcaac cttttaaggt
     8221 cacactgcga aaggtcaggt tgatccggta tcttcctgca aaagaatgat aaccttcctt
     8281 taccggcaac acgccatggt agcgcagcct cgctggtccg ccccacgcca cgacgtctcc
     8341 atgccccaac ggtattcgca tcgtcttatg ctcgcgccgc aagccacccc acaagaagac
     8401 ggccgggatt ccgagcgaaa ccgaaactat gggggaactc aagtcatgct catccttgtc
     8461 ctgatgcagg gataggcgcg cgcccggctc atagcgattg acaagacacg cgtctggccg
     8521 ataattttca aaacccgcaa gcgaggcagc atgttgcacg agattcagga aaaccggcgg
     8581 tattcgcggc cagggtttgc cgctttcggg atctatcgca tcatagcgat agcctgtccg
     8641 gtccgaaacc cagccgcagg aaccacaatt cgtcaacgcg actgacatgc gaaagcctcc
     8701 cggcgtactc atacggcgaa acggagcctc tgctgtaaca gcctgcagcg catcgagtat
     8761 ttcggcctgg tacggcagag caagcctgcg cagcacaacg gcgccagggc acaattcttc
     8821 ccgctccgag agccactgcc ccgcgtcttc atcctggaac agggccaatg tcactggctc
     8881 attgtcaaac tgaatcacga aatgatctcc aggttttgat ctccaaggca caacctcaag
     8941 tattttcatt cagctgcctc gacgaaaatc ttctccgcac ttcacggacg acgctgctgt
     9001 atctgtcgac gaccaagaaa accatgctcg ctgcctgaat ccccacgcgc agcaatagcg
     9061 gcttaaggag aagagaacag gaaatgcccg aacacgggaa agtgatcgga cagatcataa
     9121 ccgcctcccc aaagtgtccg agagcgagga attaacgtct cgatgatggc atccagcggt
     9181 tgcaggtgac ggtttgatac gagcacgtag tcgagataca aacgatagcc agcccatttg
     9241 ttgatggctg agtctacggt gtaagggtgc cccgagggct ttggatgcca tgcatccaga
     9301 attcgtagca tggcagcgta ctcaacagcg tcgtacttat ccacgttcag gtcccctgcg
     9361 atgaagaccg gctctgtgcc ggagggccct atatcatcaa cgaacgactt gataatgccc
     9421 agctgctgca tccgtgtttg ccgctgcccc atctccctgc cagcttgcgc atgagacgcg
     9481 aaaacgtgat aggtttgggc gtgcttctca aaacgtgcgt agagaacgcc tttgtcagcc
     9541 cagcagtcac ccccgacgca cacgtcgccg aagagtcgct gagtctcggc agtaataggc
     9601 caacggctta cgacgatgac gccgccatcc tgcacgaagc cgtgatcccc gccaaggata
     9661 ctcgtccgga acgggtactc ctcagccaag tcggccagga gctgatccct taccttgtta
     9721 tcgaacgcct cggagaaaac gataacgtcg aatccgcgca gcttcgaagg caacaccgcg
     9781 ccgcgttctg cctgggcatc agcaaacagg ctcgcagggc gcatataaat attgtaggac
     9841 agaacgttga agctgttgag gaggtaatgg cttcgctcaa taaccccgtc gctggcaaaa
     9901 gccgtcctga ttatgactag cagcagagcc agcatgcgaa gaaaactcaa gaaataaccc
     9961 tcgcagcaaa accagtttca gtataccggg taagaaggtg gacacttatt gaaataagct
    10021 aaacttcgtt tactgccgct ccgaaacggg cacaaactcc cgctcctccg gcccgatgta
    10081 attggctgac ggccgaatga tcttgttatc aatccgctgc tcgataatat gggcggccca
    10141 cccgctcgtt cgggcaatcg cgaatagcgg ggtgaacatg ggggtgggta cgcccatcat
    10201 gtgatagctt acggcggagt accagtccaa gttggggaac atgtttttgg cgtcccacat
    10261 tactctttcg agcctttccg ccacctcgaa cattttcata tctccggcct cggtggagag
    10321 ctttctcgct acttcccgga cgaccctgtt gcgcggatcg gagagcgtat ataccggatg
    10381 cccgaatccg ataatgattt ccttcgccgc gagacgacga cgaatgtcca cctcggcttc
    10441 ttccccgctt tggtagcgtt tctggatttc gaatgcggcc tcgttggcgc ccccatgctt
    10501 ggccccacgc agcgcaccaa ttgctccggt tatggcggag tagaagtccg agcccgtgct
    10561 cgctatcacg cgggcggtaa aagtcgacgc gttgaactca tgctcggcat ataaaatcag
    10621 ggaagtgttc atcgccctca cccaggacgg tggcggcggc ttgccatgca gcaagtggag
    10681 aaagtgtccg ccgatggaat catcgtcggt ctccagctcg attcgccgct tgtgatgtga
    10741 atagtgatac cagtaaagca acatcgaccc gaggcaagcc aacagtctgt ctgcaagatc
    10801 ccgtgcgcgg tctaaagaat gagcttcagc ttcaggaaga agcgcgccca aagcggaaac
    10861 gccagtccgt aggacatcca tggggtgcgc cgtggcggga atggtttcga gtacgatctt
    10921 caagggcgcg ggcagactgc gcagagtttt gagtttctcc atatacgcat ccagttccac
    10981 ccgcgtgggc agcttctcat gtatcagcag ataggcgatc tcttcgaatt cgcaggtttc
    11041 cgccacatcc agaatatcgt agccgcggta atgcaggtca ttaccggttc tgccaacgct
    11101 cgaaatggcc gtattacctg ctaccacgcc tgatagcaca acagattttt ttgccttggg
    11161 cgggctggtg agtacgccaa tatcagttgc ctggttcatt gccgggtttg atggatggat
    11221 cggaaaataa ttcgttcagc ttgtcttcgt atgcatggta gtcgagaaat tcatacagct
    11281 cgtcgcgagt ctgcatcgca ggaattacgc ttctctgcga gccttcgccc cgtatggcct
    11341 gatacgtttt caatgcagca gcgctcatcg cgcgaaatgc ggatagcgga tacaacgcca
    11401 gggaaacatt ggcatccgcc agttcctcga cagtataaag tggcgtgacg ccaaactcgg
    11461 tcatatttgc aaggatggga acattcaccg cggaggaaaa cttccggtag gtttccagtt
    11521 ctgtgacagc ctctggaaaa atcatatccg cgcccgcttc cacgtatgcg cgcgacctct
    11581 caatagcggc ttgcaaccct tcccccgcca atgcatccgt tcgggccatg atggcaaagc
    11641 cgggatcggt tcgagcatcg acggcagcct tgatccggtc caccatttca tcctggccca
    11701 caagcacttt gcttggccgg tgcccgcaac gctttgcctg tacttgatct tcgatatgca
    11761 tcgcccctgc cccggcttta ataagcgacc tcacggtgcg agcgatgttg aaggcgcttg
    11821 gaccaaaacc ggtatccgca tccacgagca aaggcaggca ggtcacgtcg gtgatccggc
    11881 gcacatccgt cagcacgtcg tcaagggtgg aaataccaag gtcgggcatg ccgagcgaag
    11941 cggccgctac ccctcccccc gagaggtata tcgccttgaa gccggtgagt tcagccaggc
    12001 gagcggcata tgcattaatg cagcccacga cctgcagagg tctctcctgg gcgatggcct
    12061 cacgaaaacg ataaccgggc gaaacagtca tcaacttcct accaaaccgg cgcgaaactt
    12121 acgcttcgcc cagcagatgc ttcacggacg ctcgctcatc caccagttcc tggtaggtga
    12181 tgtccatttt ttttcgatcg gtttcactga tctccaggcc agacacaagc cggtaagcgc
    12241 cgtcgcggca gacaacaggc gaaccgtaca tcacaccctc cggaatgccg tagctcccat
    12301 ccgaaggaat cgccatggac gtccagtcgt cctcccgagt accgaacacc cagtcgcgga
    12361 catgctcgat cgctgcgttc gccgcgctgg cggcgctgga tagtccgcga gccgcgatca
    12421 cttccgttcc acgattctgc acggtgggaa tgaaatggtt ttctatccac ccggaatcct
    12481 ggatgaggtc actgaccttg tcgccaccca cctccgcgtg gctgaggtcc gggtattgag
    12541 tggatgagtg attcccccag acaatcattt ttcggacgct ggaaaccggt ttttcaagct
    12601 tcgcggccac ctgccataag gcccggttat ggtcgaggcg catcatggcc gagaaattgc
    12661 gaggattgag accgcgcgca tttttcatgg cgatataagc gttagtattg gcgggattgc
    12721 cgacgaccag tacctttaca tctcgccgcg cagcctcgtc cagcgccctc ccctgggtgg
    12781 caaatatgac gccgtttgct tccagcaaat ccctgcgctc catgcctttg ctacgggggc
    12841 gcgcacccac caggatggct atgccggcat ctttgaaagc cgctttcgga tcatcggttg
    12901 ttgtgacccc tgcaagcaag ggaaacgcgc aatcctccaa ctccatcaat acgcctttga
    12961 gcgagggcag caattgcggt atatcgagca attgcagaat gaccggctga tccctcccca
    13021 acatgtcccc cgccgcgatt cgaaacagga gactgtaacc gatctggcca gcggcgcctg
    13081 tgaccgcgat gcggacggga gtgttcattc ttgcgctcct gtaggagatt gccggtaaga
    13141 aaactgtccg gatagctgag ctctcgggac tcgagcacga tagctggatg agcttgtttc
    13201 ctccacaggg cggctaatgc cagaacctat ggtcggaacg catccatgtg tggcaggcaa
    13261 attgtactga ctatagaaac attcatcggg gctgtcaatt caccaagtcc ctttacggaa
    13321 ccaagcctca tactccactt gtagttgagc cagagctatc ctgctggaga tgaatgacgg
    13381 caccgtccgg atgagcttgg ttgtatcccc agttataacc gtctaaactt caactggtct
    13441 agcatgggaa aagcgacaat tgcagaaaaa gcgcccaaaa tacctgaacc tcctggaaat
    13501 caggcagcct ttgcccgcga tcgtttcaat cctgcatcgt atcagcggcg cgctgctgtt
    13561 tttcccgggc atccccctgc ttctccttgc gctggagagc ttgttgagtt ctccacaaac
    13621 ctacgcccag tttcagtcgg tcttcgctca tcctttattt aaagcggcat tcgtgcttgc
    13681 cgtgtggctc ttcctgcatc acttgctcgc tggaatccgt ttcctcgggc tcgacctcca
    13741 ctatgcgatc gagatccacc aggcgcgtct gacgagcaag ctcgtcctcg ccgcaggaat
    13801 cgtcctgacc cttctgatcg gaattgcaat atggtgaggc gggtcgttac gggcgcgcac
    13861 tacggcctgc gcgactggct atcccagcgc gtcactgcgc tggtaatggt cgtctatgtt
    13921 ttatgcctgg tggcaggcct gctgctctct ccgcctcggg actatgcagg atggaaagcg
    13981 ctattcagca atgagtggat gcggacagcc tcgctgctct ttctgctcag cctgttctgg
    14041 cacgcgtggg tggggatgcg caacattctg atggactatg tacatgcaac cggccttcgc
    14101 ctgacgctac aggtactggt tattctttcc ctggtttttt acgccatctg ggcaacggaa
    14161 attctatgga tgtcctgaag acggatgaaa gccggatcga gctgacgtgg caataggtca
    14221 gagaaaattt gacgttgtca ttgtgggcgc gggcggtgcg ggaatgcggg cagcgattct
    14281 gctatcggaa gcaggtctca aggtcgccgt gctatccaag gtattcccca cccgctcgca
    14341 caccgtggcc gcccaaggtg ggatcgcggc ggccctcggg aatgtgacgg aggattactg
    14401 gtactggcac atgtacgaca cggtgaaggg ctccgattat ctcggcgacc aggacgccat
    14461 cgaggtgatg tgccggcagg cgagcgaagt tgtgtacgaa ctggaacact acggaatgcc
    14521 gttcgatcgc ctcgagaatg gaaaaattta ccagcgcccg tttggaggtc agtcgcaaaa
    14581 ttttggagga gcgcaggcga cacgctcgtg cgcagttgcc gaccgcaccg gtcacgcgat
    14641 gctgcacacg ctctatcagc gcaatgtccg cgccaacacc cagttttatg tcgaatggat
    14701 gggactggat ctgatccggg acgaagaggg ggacgtttgc ggagtgactg cactggacat
    14761 cgaaacgggc gaagtcatga tactgcaggc gcgtgccaca ctctttgcga ctggcggggc
    14821 cgcccgcatc ttcagcgcca gcacgaacgc attcatcaat accggtgacg gactggggat
    14881 ggcagcgcgt gcgggagttc cgctcgaaga catggaattc tggcagtttc atcccaccgg
    14941 agtccatggg gtgggcgttc ttatcagcga agcggtacga ggagagggcg gttatctggt
    15001 caacaagaat ggcgagcgct tcatggaaca ttatgccccg cacgcaaagg acctcgccag
    15061 ccgggatgtg gtttcccgcg cgctttcgac ggaaatcaag gaaggacggg gatgcggaaa
    15121 ggatgccgac catctgctgc tgaaacttga ccatcttggc ccggaagtga tcacgacgcg
    15181 cctgccagga attcgggaaa tcgccatcaa gtttgccaat gtagatccga ttcatgaccc
    15241 gattcccgtc gtacccactg cacattacat gatggggggc attcccacca actattatgg
    15301 ccaggttgtg gcgccataca agaccagccc ggaggaagtc gtccagggat tttatgcagc
    15361 cggagagtgc gcgtgcgttt ccgtccatgg cgccaaccgc cttggcacaa actcgctgct
    15421 ggacatcctg gttttcggcc gcgctgccgc aaatcagatc atcgaagacc tgaaaaaaaa
    15481 ctcgcatcac aaaccgttgc cagcaaatgc tggcgataag gcacttgccc ggctcatgcg
    15541 gctggacagt caaaaagagg gtgaaaacgt ggctcaggtc ggcgatgcaa tgcgcaggac
    15601 gatgcagaca cattgcggcg ttttccgttt tcccgatttg ctgaaagagg gcgtcgcgat
    15661 gattgaagag atcgcggaaa gggccaggaa cacagtgatc ggcgacaaga gccgtgtttt
    15721 caacactgcc cgggtcgagg cccttgagct cgataacctg gtggaagttg cggtcgcaac
    15781 catggtttcg gcagaagcgc gaacagaaag ccgcggtgcc cacgcacgcg aggattttcc
    15841 cttccgggat gacaataaat ggctcaagca taccctttat tttgccgaag ataggcggct
    15901 cgattacaaa ccggtgcggc tgaagccgct gacagtggag tcgttcccac ccaagccgag
    15961 aacttattga ctcgatagca ctcgactatg gaacaaccct gggaatatcc atgaaatttt
    16021 ccatataccg ctatgatcct gacaaggatg acaagcccta catgcgggat tatgacgttg
    16081 cgctggagcc taccgacagg atgctgctcg atgcgctggt acgcatcaaa acgatggatg
    16141 acagcttgag cctgcgccgt tcctgccggg aaggcgtatg cggatcggac gccatgaata
    16201 tcaacgggcg caatggattg gcatgcatca cgcctgtgaa ggacctgaag gagcctgtca
    16261 ctctgcaccc tctgcccgga ttgccggtga tccgcgacct ggttgtcgac atgagccaat
    16321 tcttcaagca gtatcattcg atcaaaccct atctcatcaa taatgatcct ccgccggaaa
    16381 ctgagcggct ccagtctcct gccgaaagag cggagttgaa tggtctttat gaatgcatcc
    16441 tgtgcgcctg ctgctcgact gcttgcccat cgttctggtg gaatccggat aaattcgtcg
    16501 ggcctgcggg actgctgcag gcatatcgct tcatagcgga cagccgcgac caggcaacgg
    16561 cagaacggct ggattatctc gaggatcctt accgcctgtt ccgctgccat tccatcatga
    16621 attgcgcaga tgtatgcccc aagggcttga atcccaccag cgccattgaa agcatcaagg
    16681 atatgatggt caagagaaca gtatgaagaa tcaggagcgg gcgcgctggc gttgccgaag
    16741 aggcctgctc gagctcgaca ttgttttgca gcggttcgtc gatatccatt acgaaaaact
    16801 tgacgaggaa gggctccggc aattcgaggc tttgctgggt cttgccgata acgacttatg
    16861 ggatatgatt gctataaagg aagcggcgga taataacgtc gaccccgtgt tgcgcctgct
    16921 tcgcgccgtt tgacgttttt gatcgctgct ccggtcatta taaagatcgc tgctccggtc
    16981 attataaaaa tacgaggaac aaaaatggaa gacaaccgca ctgccacgct cgatctgcac
    17041 aatgaggcgg ggccgctcga atttccgctg ctagctggca caatgggtcc cgatgtagtg
    17101 gatattcgca ccctttattc caaggcaggg gtctttacct acgatcccgg cttcctttcc
    17161 actgccagca acagttccgc gatcacgttc atagatggcg ataaagggat tttgctctac
    17221 cgcggttatc cgatcgaaca actggccgtg caatgcgatt tcctggaggt gtgctacctg
    17281 ctgctgaacg gcgaattgcc gaccagcgaa cagaaagcag cgttcgacca taagatcaag
    17341 gatcacacga tggtgcatga acagctcgcc cggttctaca cggggttcag gcgcgacgcg
    17401 catccaatgg cggtcatggt gggggtcgtg ggcgcattgt cggcgttcta ccatgacgca
    17461 atggatattg cagatgcctc gaaccgcgag ctgtcagcct tccggctgat tgcaaaactg
    17521 cctaccatta ctgccatggc ctacaagtac aacatcggcc accccttcat ttatccgcgc
    17581 aacagcctca gctacgcgga aaatttcatg cacatgatgt tcgcaacccc agcggaggaa
    17641 tataagtcca atccaatcct cgcgcgggca ctggacagga ttctgatcct gcatgcggac
    17701 catgagcaga acgcttccac atccaccgtg cgccttgcag gttccagcgg ggcaaaccct
    17761 tttgcctgca tttctgcagg tattgcctgc ctatgggggc ctgcgcacgg tggggcgaac
    17821 gaggcttgtc tgaacatgct gcaacagatc ggcaacgagt cgcggatcgg cgagtatatc
    17881 gagcgggcaa aagacaaaaa cgaccccttt aggctggttg gattcggtca cagggtctat
    17941 aaaaatttcg acccgcgcgc gaaactgatg cgcgagacct gctacgaagt attggatgag
    18001 ctcgggctac acgatcatcc cctgttcaag ctggcgctca agcttgagaa gattgccctg
    18061 gaggatgaat acttcatttc gcgcaagctg tacccgaatg tcgattttta ttcaggaatc
    18121 gtgcagcaag cgctggacat tccgccatcg atgttcaccg caatatttgc catggcccgt
    18181 acggcggggt ggattgctca atggaacgag atgatctccg atccggaaca caagatagga
    18241 cggccgcgtc agctttatgt aggggccacc acgagggatg tagtgccgat cggatcacgc
    18301 aaataaacgg ctctgccgga cgaagaggta tttggccatg tcacaggggt tatcgacgcg
    18361 ctctaccgac tcagtactat tcggcggaaa tgctcctttc atcgaggagc tttacgagga
    18421 ataccgccgc gacccgtatt cggtcacacc cgaatggcac gcgtacttcg atcagctgca
    18481 gcaatccggg caagcagcaa ggtcatccag cgccctttcc cctcagacgc ctcgacgggt
    18541 aacccctgcg actccagcag aactcgacga gcgggcgctg acaatggagc gcaagcagat
    18601 tggcgtcctg cagctgatca acgcctaccg ttttctgggc gtgcgccacg caaatctcga
    18661 tccgcttggg catcaggaaa aaccgcacgt gccggaactc gatcccgcct attatgggtt
    18721 gacagatgcg gatatgacta cgatatttgg caccggctcg ctggtcggag cccctcgtgc
    18781 cccgctcggc gacatcatcc aggtgctgga aaagacctat tgcggcagta ttggcgtaga
    18841 gtacatgtac atcagcgata cggagcagaa acgctggatc cagaaccgta tcgaggggcc
    18901 gcgcgcccag cccgactatt cggtggaata caagcgtcat atcctcgaga ggttgactgc
    18961 agcggaaggc ctggaaaaat acctgcatac ccgctacgtc gggcaaaagc gcttctccgg
    19021 cgaaggtggc gaaagcctca ttcccctgct ggacaatctg ctgcaacggg cggggcagat
    19081 gggcgtacag caacttgtga tcggtatggc ccatcgcggc cggttgaacg tgctggtcaa
    19141 caccttgggg aaggttccag ccgacctgtt ccaggaattc gaaggcaagc atccccagga
    19201 attgaccgaa ggagacgtca agtaccatca gggcttttcc tcggccgtat cgacaccggg
    19261 tggcgtgatg cgagtttccc tggcattcaa tccctcgcat ctggaaatcg tggatccggt
    19321 ggtagagggt tcggtacggg cgcggcagca ccggttgggc gacaaggaag gcgatctggt
    19381 gttgcccgtc ctgctgcacg gcgacgccgc gtactccggg cagggagtgg tcatggaaac
    19441 cctcaatctg tctcagaccc gtggttttgg aaccggagga acagtccacg tcatcatcaa
    19501 caaccagatc gggtttacca cttccgaccc gcgcgatgcc cgctcaacac tttactgcac
    19561 cgatgtctcg aagatgatcg aggcgcctat tttccatgtg aacggggatg atcccgaagc
    19621 cgtgctcatg gttacggaaa tagcgctcga tttccgcgtg agattccata aggatgttgt
    19681 cgtcgatatg gtctgcttcc gaacgttggg gcacaacgag caggatgaac cgatggtgac
    19741 ccagccgctc atgtacaaga tcattgccaa acactccggc acgcgcgagc tttatgccca
    19801 aaagctggaa gccgagaagg tgataacccc tggcgacgca gagcgtatgg taaaaagcta
    19861 tcgcgacgat atggatgccg gccgcaatcc caacaagacc gtcgtttatg actataaagc
    19921 gccggacgca gtggattggt cacctttcct taagaatacg ccttgggata ttccggtcag
    19981 gaccggactt cccatggatg aattgaagaa gctggccgaa tgcctgacgg acatccccgc
    20041 caaattcaag ctgcatccgc gcgtcgagaa aatcatcgcc gaccggcgcc tgatggccga
    20101 gggaaaattg ccgctcgatt ggggcatggc ggaaacactt gcttatgcag cgcttttaaa
    20161 ggatggttac ggcgttcgca tctcagggca ggactcgggg cgcggaactt tttttcaccg
    20221 ccacgccgtg ctccacgacc aggatcggga gcgatggaac gaaggggtct atattcccct
    20281 gcgtcatata gagccaaacc agccggattt taccgtgatc gattcgctgt tatccgaaga
    20341 agcagtgctt gccttcgaat atggctatgc aacggcggaa ccggatgagc ttgaggtttg
    20401 ggaagcccag ttcggcgatt ttgccaacgg ggcgcaagtc gtgatcgatc agtttattgc
    20461 gtccggcgaa gccaagtggg gccggctgtg cgggcttgtc atgatgctgc ctcacgggta
    20521 cgagggacag gggcccgaac actcttcggc gcgcctggag cgctatttgc aattatgcgc
    20581 ggaatacaat atccaggtat gcgtaccctc cacccctgcc caaatgtttc atgttctgcg
    20641 tcggcagatg atccgcccgc tccgcaaacc gcttattctc atgacgccga agagcctgct
    20701 gcggcacaag gaatcggtgt cgagccttga ggatatagcc gatggaagct tccagcctgt
    20761 cattcccgat atgggaaacc tggatgcggg caaggttcga cgcctgatcg cctgctcagg
    20821 aaagatttat tatgacttgg cggcttaccg ccgggaacat aagatcgaag atgtcgccat
    20881 tatccgcatc gagcagttgt acccctttcc tcacaaggat ctccagcgcg aggtcgagcg
    20941 ctatccgaac gcgagggaca tactttggtg ccaggaagag cccggcaacc agggagcatg
    21001 gcaccgtatc cagcattacc tggcgcggca caagcggccc gatcaggtct tgggttatgc
    21061 attgcgcccc tcttcggcat cgcccgcagt aggttatttc gccaaacaca atgatcagca
    21121 aaaaaaagtg gttgccgctg catttcgcga gaaaatctga caagaggtga tgatgagcgt
    21181 cgaagtcaaa gtcccattgc tgtccgagtc ggtttccgaa gcgacccttc tcaaatggca
    21241 taagaaggaa ggcgactatg tggacaggga tgagaacctg atcgatatcg aaacagacaa
    21301 ggttgtgctt gaggttcccg ccccggcggc cggagtgctt gcaaaaataa taaagggcga
    21361 cggggcaacc gtcacaagtg gagaggtcat cgcatcaatc gataccgaac cggcaaaaga
    21421 caccgcagtg cgaggcgcgc agccctcgtc acagcgggaa acgggaactg ggcaggcggc
    21481 caggaagcag atgtcatggc ccgagcccca gtcccagcgt aagccatctc ctcctccccc
    21541 tgccggagcg cctgcacaca tgatgccggc tgcacgtggg gcagctgcag cggccgggct
    21601 tggcgcagcc gaagtgggtt cgatcaaggg cagcggccgg ggtggcagga tcacgaagga
    21661 agatatcgta gcccacgtgg agcacaagtc tcccccagca cctccgcctg tcgagtcggg
    21721 acctcccgcc gtcgaacccc cgcctcctcc gagcgacaag cagccaaccc ccgctccggc
    21781 cccttccgcc aagcccattt ctccacccgc tccgcccgct caaccggcgg gcgatcggct
    21841 tgagcggagg gaatcgatgt cgcggttgcg cgcccgtatt gccg
//
LOCUS       DASSHJ010000013         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1297236,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000013 DASSHJ010000000
VERSION     DASSHJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297236"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..599
                     /locus_tag="VFI43_00855"
     CDS             222..599
                     /locus_tag="VFI43_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060708.1"
                     /translation="MPTSKPRITITLTEYQHAILRRISESSGRSMSGVISEFMLAAQP
                     TLERMAHTFQQLKQDRDEDHQRVAGLLAGLNADRDRKILCDQSDVRAERGEKNWGNET
                     TQGERDYSGGPADDQSTPEHLKQ"
     gene            789..1610
                     /locus_tag="VFI43_00860"
     CDS             789..1610
                     /locus_tag="VFI43_00860"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OBAP family protein"
                     /protein_id="HET7060709.1"
                     /translation="MKTRATNSPCSIFLSAFLFPVVLACGWAGSSLAEEPGPGIAKTI
                     PAGEEKTPKTRLLEAGAKLLQSKTPLKGFDIYLVGFHPMKESPNEQMEAHHYCRQVNE
                     DFAQCVLFASNDKNASLNGIEYIISERLFNTLPEKEKKYWHPHDGEILSGQLVAPGIP
                     EVAEKALMKEKMNSYGKTWHLWNTGFNDKPGDELPIGEPMLGWSFNREGEALPGLVER
                     RDEKMKIDTAEKRRQRAELKQLAKPQSGVDDLKGKFGRPTQEIPGVVDQKQATGD"
     gene            complement(1747..2940)
                     /locus_tag="VFI43_00865"
     CDS             complement(1747..2940)
                     /locus_tag="VFI43_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET7060710.1"
                     /translation="MRTAFLVAVALAVDISHLAYAQRESVSHAAGFSVTYIGQQIVPG
                     DMRFKGTVVGGLSSIDYNPRTGHYLVVSDDRSKKNPARFYELSLDLAKFRRSEAPGMS
                     GVTFHSFTVIQRPGGEPFKKNSIDPEGLRFDTARNKIYWSSEGRRGLFSSDNPTVSEM
                     NPDGSYSRSFTVPSYYFPRGSELGILPGNTGIYNNLGFESLAISPDGETLYTATENGL
                     IQDSPPATAFSGSRARILAFDISSGRSSEEYIYEVEPVIETPPLLRPFAINGLTDFVA
                     TGNRQFITVERSFTAEAVIPGGSTGYKIRLFYADARGANNVSAMESIAGRGIVPVRKW
                     LLLDLSDLKNADGSALSVGNIEGITFGPDFNGRHTLILVADNNFSTFQLTQFIALELN
                     PIRGN"
     gene            3054..3920
                     /locus_tag="VFI43_00870"
     CDS             3054..3920
                     /locus_tag="VFI43_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060711.1"
                     /translation="MMTALAIRAYRYALSGVAIVLLVSIFAGCASFREITAFASLSSS
                     AAANNAVVKDYIGSIDRRKQYEPEKFHRELDAQKARREAQRASLDMLQQSVTAYMQAL
                     AGLTSGNARSNDESLEEFSERLNQVALLSDTEKDAAYALSTLLSRTVTSVYREHEVKK
                     LIRDANQPLQEVLAATGRITGMDIVADLRGERALVERYYDNFMLAPGNPDEPVAMALA
                     REARVRALDRVDSKIQATQSYQIVLGKVARGHQYLYEHVDRIGDHELDRHFQPYVDEL
                     EAAYREFVEVAM"
     gene            3934..4359
                     /locus_tag="VFI43_00875"
     CDS             3934..4359
                     /locus_tag="VFI43_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060712.1"
                     /translation="MVKLTAEEAFELGNRIRDAAVALHRWRIENRDRLSRSQWEELDN
                     REVTLLNFASSNFTNGIGMRLDSSQISASRLEGSVKHAASAIEHIQSFKQVLDLASAL
                     ALFAGAVASGNVAEIPAGIVALEDALSAIDHPNRPQDDE"
     gene            4494..5522
                     /locus_tag="VFI43_00880"
     CDS             4494..5522
                     /locus_tag="VFI43_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET7060713.1"
                     /translation="MTKKNMLLLAGAAIIAIVGAIWYFSRQQSSPEELVLYGNVDIRQ
                     VSLAFNGSERVAEMRVQEGDRVVADQVLAKLDTRTLALQIAQAEAQIEVQQQVLLRLK
                     HGTRPEEVAQARAEVAAARADADLAAQQLRRLRDINRNSGGQAVSQQDFDAARARRTV
                     TRAQLETRKKALQLARIGPRQEDISQAEAQLEVSRANLALLKHQLSLYELKAPIDAVV
                     RSRLLEPGDMASPQRPVYALAITNPKWVRVYIREADLGLIKPGMSAGVTIDSYPDRSI
                     EGRIGYISSVAEFTPKTVQTEELRTSLVYEVRVYVDDPKDDLRLGMPATAHIPLPGPN
                     DKNLASYP"
     gene            5519..>6343
                     /locus_tag="VFI43_00885"
     CDS             5519..>6343
                     /locus_tag="VFI43_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7060714.1"
                     /translation="MNHRPALVARGLCKRFLRKETRQTITAVKDVSLDVGAGTITALV
                     GPDGAGKTTLLRMITGLLTPDDGQLTVLGIDVVAHPQEVQNRISYMPQRFGLYEDLSV
                     QENLDLYADLHGVGQDVREARFKKLLHMTDLARFTSRPAGKLSGGMKQKLGLACTLVH
                     PPELLLLDEPTAGVDPLSRRELWEIVEQMVQGERLTVLITTAYLDEAERCAKVFILLE
                     GKLIASETPEQIRRLALNLCFVATPPPGQPPRFLQSRLLDQTDYVVDAVPEGGAVRY"
BASE COUNT         1535 a         1643 c         1832 g         1333 t
ORIGIN      
        1 ccagccgtta acgggcatgc ccaggttgtt tccgagtcgt ccggttttca tcaatagcga
       61 cgagaataga catctcaata agacggagct agaccccatt cactcataat tctattgacg
      121 aaaattaggt gatgcactag catctggtgc atctggtgca tcaccaggaa catcaccagg
      181 aacgtaagtt ttcgagctaa cgaaaacaat aaggaggact catgccaact tcgaagccac
      241 gaattaccat caccctcacc gagtatcagc acgcaatcct gcgcaggatc agtgaatcaa
      301 gcggacgcag catgagcggg gtgatctcgg aattcatgct ggctgcgcaa cctactttgg
      361 agcgaatggc tcataccttc cagcaactca aacaggaccg tgacgaggat catcagcgtg
      421 tagctggttt gctggctgga ttaaatgcag accgcgaccg aaaaattcta tgcgaccaga
      481 gtgacgtgcg agccgaacgg ggcgaaaaga actggggaaa tgagacaacc caaggggagc
      541 gagattattc cggtgggccc gccgacgatc agtctacccc cgaacatctg aaacaataaa
      601 gcctccgttc tcctcctgcg aaacctcagc ccctccgcca agcgctgttc ggaacaagtc
      661 gtgaggacag tcactgagaa tgcgctacgt tgaagttcac gcggcaggga tttgaaaagg
      721 aatacggccc cggtcatttg tatgatgccg caccgacgat acgctggacc tgcctcgaca
      781 atggttgcat gaaaacccgc gccacaaaca gcccgtgcag tatttttctc agcgcttttt
      841 tatttccggt cgtccttgca tgcggatggg ctgggtcatc cttagctgag gaaccggggc
      901 cgggtatagc gaaaacaata cctgctggcg aggaaaaaac tccgaaaacc cgattacttg
      961 aggcgggtgc gaaactcctc cagagtaaaa ccccattaaa aggcttcgat atctacctgg
     1021 tcggctttca tccaatgaaa gagtctccca atgaacagat ggaggcgcat cattattgcc
     1081 gccaggtcaa tgaggacttc gcgcagtgcg tattgttcgc cagcaacgac aagaatgcca
     1141 gcctgaatgg aatcgaatac atcatttcgg aaaggctttt caatacgtta cccgagaagg
     1201 agaaaaaata ctggcacccg cacgatgggg agattctttc cgggcagctc gtcgcgcctg
     1261 gcatacctga agtggcggaa aaagcgctga tgaaagaaaa aatgaacagc tatggcaaga
     1321 cctggcattt atggaatacc ggtttcaatg acaaaccggg tgacgagttg ccgataggcg
     1381 agccgatgct cggctggtcg ttcaaccgcg agggtgaagc cctgccaggg ttggttgaaa
     1441 ggcgggatga aaaaatgaaa atcgacaccg ctgaaaaacg caggcagcgg gcggagctta
     1501 agcaattggc caaaccgcag tcaggggtgg atgacttgaa agggaagttc ggccggccta
     1561 ctcaagagat tcctggagtg gtggatcaaa aacaggccac cggagactga tcaggcagga
     1621 accaaggctt tgcggaagcc gtagggaagg gattccctcc gcgtgttgtc actcccatcc
     1681 tgcttgtaca cgtaatctgt ctaccgctcg gtgggtctcg atagccatca gcacatcatg
     1741 taaccactaa ttcccccgaa tggggttcag ttccagtgcg atgaattggg tgagttgaaa
     1801 ggtggagaag ttattatctg ctacgagtat aagcgtgtgc cgcccattga agtccggccc
     1861 aaaggtaatg ccttcgatat ttcctacgct cagcgctgat ccatccgcgt tcttcagatc
     1921 ggacaggtcg agcaatagcc acttgcgaac gggtacaatc cctctaccag cgatggattc
     1981 catggctgag acattgttcg ccccacgagc gtcagcataa aatagacgga tcttgtagcc
     2041 cgtggatccg ccggggatca ctgcctccgc ggtgaacgaa cgctcgacgg tgatgaattg
     2101 cctattccca gtcgcgacaa aatcagtcag gccattgatt gcaaatggtc gaagcagggg
     2161 cggagtctcg atgacgggct caacctcata gatatattcc tcacttgatc ttccgctgga
     2221 aatatcgaaa gcaagaatgc gcgcgcgcga accactgaaa gctgtggccg gcggagagtc
     2281 ttggatcaga ccattttccg ttgcagtata tagggtctca ccatcaggag agattgccag
     2341 gctttcgaat cccagattgt tataaatgcc ggtatttcca ggaaggatgc caagttccga
     2401 tccgcgcgga aaataatagg aggggacggt gaaactgcgg ctataactgc cgtccggatt
     2461 catttctgaa actgttgggt tatcggaact gaaaaggccg cgcctgcctt cgctgctcca
     2521 gtagatcttg ttgcgagccg tgtcgaagcg caggccttcg ggatcaattg aattcttctt
     2581 gaaaggctcg cctcctgggc gctggataac ggtaaaggag tgaaacgtca ccccggacat
     2641 gcccggcgct tcggagcgcc gaaatttcgc gagatccaat gataattcgt agaagcgggc
     2701 aggattcttt ttgctcctgt cgtcgcttac gacaagatag tggccggtgc ggggattgta
     2761 atcgattgaa gacaaaccgc cgacgaccgt tcccttgaag cgcatatcgc cgggtacgat
     2821 ctgctggccg atgtacgtga ctgaaaaacc cgccgcatgg gacaccgatt cgcgctgtgc
     2881 gtaagccaga tgcgagatgt cgacagccag ggcgacggca acgagaaacg cggttctcat
     2941 gcaggtatac tcatcaatta ggggggttgt ttccccagcc aagtttatag ctcaaagctg
     3001 cggcagagct actgcttcta gaataaaatg acttattact cacgatggcg aagatgatga
     3061 cggctcttgc aattcgcgca tacaggtacg ccttatctgg cgtggcaatc gtcctgctcg
     3121 tgagtatttt cgcaggttgt gcgagcttcc gcgaaatcac tgctttcgca tcgctttcct
     3181 ccagcgcggc tgccaacaac gctgtggtga aggactatat cggatcgatc gatcggcgca
     3241 agcaatatga gcccgagaag tttcaccgcg aactggacgc gcaaaaggcc cggcgtgagg
     3301 cgcaacgtgc ttcgctggat atgctccagc agtccgtaac ggcgtatatg caagcgctcg
     3361 ctggtctcac gagcgggaat gccagaagta acgacgaatc ccttgaggag ttcagcgaaa
     3421 ggctcaacca ggttgccctg ctgagcgata ccgaaaaaga tgcagcgtac gccttgtcca
     3481 ccctcctttc ccgaacggtg accagtgtat atcgcgagca tgaagtcaag aagctgatcc
     3541 gtgatgccaa ccaacccctc caggaggtgc ttgcagccac cggaaggatt accggcatgg
     3601 atattgtcgc ggatttgcga ggggaaaggg ccctggttga gcgatattat gataacttca
     3661 tgctagcgcc cggcaacccg gacgagccag tcgcgatggc gcttgcacgt gaagcgcgcg
     3721 ttcgggcact ggaccgcgtg gatagcaaga tacaggccac gcagagttac cagattgtac
     3781 tgggtaaggt ggcccgaggt catcaatacc tgtatgaaca cgtggacaga attggcgatc
     3841 atgagctgga tcggcatttt cagccttacg tagacgagct ggaggctgcg taccgcgagt
     3901 tcgttgaggt tgcaatgtga acccgggaag aacatggtca aactgacggc cgaagaggct
     3961 tttgaactgg gcaaccgcat acgggatgca gcagttgcac tgcacaggtg gcgtattgag
     4021 aatcgcgatc gcctgagccg atcgcaatgg gaagaactgg ataacaggga ggttacgctt
     4081 ctcaattttg cgagcagcaa ttttacaaac ggaataggaa tgaggctgga tagcagccag
     4141 atatccgcct ctcgcctgga aggaagcgtg aagcatgccg catccgcaat tgaacatatc
     4201 caatccttca agcaggtact cgatcttgct tctgcgcttg ctctttttgc cggagccgtt
     4261 gcgtcgggaa atgtggcgga aatcccagcc gggatcgtcg ccctcgaaga tgcgttgagc
     4321 gctattgatc accccaaccg cccgcaggac gacgaatagc ggggcagcac gcccttagct
     4381 tatatttttt ggtaattaat ggctgcacaa gcttgaaaga ggaggctatc aaaaaggctg
     4441 gcgaagccga tggcgggcct gggaagaagg accaggcccg ccattctggg ctcatgacaa
     4501 aaaaaaacat gctcctcctt gccggggctg ccattattgc catagtagga gcgatttggt
     4561 atttcagcag gcagcagagc tcgcctgaag agctggtgct gtacggcaac gtggatatac
     4621 ggcaggtttc gctcgccttc aacggcagcg agcgggtggc cgagatgcgg gtgcaggaag
     4681 gtgaccgtgt cgtagcggac caggtactgg ctaaactgga tacgcgaacg ctggctttgc
     4741 agatcgctca ggccgaggcg caaatcgagg tgcagcagca ggtattattg cgactcaagc
     4801 atggaacgcg gcctgaagag gtggctcagg cgagagccga ggtggctgcc gctcgggcgg
     4861 atgccgatct tgctgcccag caactccgac ggctccggga tatcaatcgg aattcgggag
     4921 ggcaagcagt cagtcagcag gattttgacg cggctcgcgc ccgtcgcacg gttacccgtg
     4981 cgcagctgga gacgcgaaag aaagcgctgc agcttgcgcg gattggacct cggcaggagg
     5041 atatttctca ggcagaggcg cagctcgagg tatcccgggc taatttagcc ttgctgaagc
     5101 atcagctcag tctctatgaa ttgaaggctc cgatcgatgc cgttgttcgt tcccgccttc
     5161 ttgaacccgg tgatatggct tcgccgcagc gaccggttta cgcattggcg atcaccaatc
     5221 caaaatgggt tcgcgtctat ataagggaag ctgatcttgg cctgataaag ccgggaatga
     5281 gcgcaggcgt gaccatcgac agctatcctg accgttctat cgaggggcgt attgggtata
     5341 tctcctcggt ggccgagttc actcccaaaa cagtgcagac tgaagaattg cgtaccagcc
     5401 tcgtctatga agttcgggtt tacgtcgatg accccaagga tgatcttcgg ctaggcatgc
     5461 cggcgaccgc gcatatccct ttaccgggcc cgaatgacaa aaaccttgcc agctacccat
     5521 gaaccatcgg ccagcgctcg tcgcgcgagg gctttgcaag cgatttctca ggaaagaaac
     5581 caggcaaacg atcacagcgg taaaagacgt ctctctcgat gtgggggcag gtacgatcac
     5641 tgctttggtg ggccccgatg gggcaggtaa aaccacgttg ctcaggatga taaccggtct
     5701 tctgacgcct gacgacggcc aacttacagt cctgggaatc gatgtcgtgg cccaccccca
     5761 ggaagttcaa aaccgcatta gctacatgcc tcaacgcttt ggtttgtatg aggatttgag
     5821 tgtacaggaa aacctggacc tctatgcgga tcttcacggt gtgggtcagg atgtacggga
     5881 ggcgcggttc aaaaagcttt tgcacatgac ggatctggcc cgcttcactt cgcgtccggc
     5941 tgggaagctg tcagggggga tgaagcagaa gctggggctg gcctgtaccc tggttcatcc
     6001 accggaactg cttctgctgg atgagccgac tgcgggagtc gatccgctgt cgcggcgtga
     6061 actgtgggag atcgttgaac aaatggttca gggtgaacgc ttgaccgtgc ttatcacgac
     6121 cgcatacctc gatgaggccg agcgctgcgc aaaggttttc attttgctcg aggggaagct
     6181 gattgcctcg gagacaccgg agcagatacg ccggctcgcc ctcaacctgt gttttgtcgc
     6241 gactccgcca ccaggccagc caccccggtt tctacagtcg cgcctcctcg accaaacgga
     6301 ttatgtggtg gacgccgttc cagagggcgg cgctgtccgg tac
//
LOCUS       DASSHJ010000014         7806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_534671, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000014 DASSHJ010000000
VERSION     DASSHJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534671"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..444)
                     /locus_tag="VFI43_00890"
     CDS             complement(280..444)
                     /locus_tag="VFI43_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060715.1"
                     /translation="MHAVSIQLEALDVLPHVADTTIKSFKGIVIFDIEHGVDIAVYEK
                     CVCEESHRES"
     gene            626..922
                     /locus_tag="VFI43_00895"
     CDS             626..922
                     /locus_tag="VFI43_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HET7060716.1"
                     /translation="MASVGYHEPIEELSDETRDMHRAITSLMEELEAVDWYNQRISAS
                     KDAQLKAILTHNRDEEKEHASMLLEWIRRRDHVFSNELKEYLFNENPIVHEQKT"
     gene            complement(1060..1965)
                     /locus_tag="VFI43_00900"
     CDS             complement(1060..1965)
                     /locus_tag="VFI43_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET7060717.1"
                     /translation="MISKLVIIGVGLIGGSLALALRQAGEVQYIVGMGRSPENVQRAL
                     KLGAIDEIGADFQSALKNAEIVFLSVPVGQTGAIMSEIAPHLQSGTIVTDAGSTKQNV
                     IIAAKNHLASHLAMFVPGHPVAGAEQSGAGAARPGLFRGKNVILTPGDETSESATKRV
                     IELWQACGARVSLMNAARHDEVLASVSHLPHVLAFALMHRFYASNLHSRDESGVDEAL
                     GPGDALGFAGSGFRDFTRIAESSPEMWRDICLANQDALCNEIDAYQHELAEIREMLAR
                     GDSNSLKQVFSDARQVRRNWLESLS"
     gene            complement(2035..3051)
                     /gene="aroF"
                     /locus_tag="VFI43_00905"
     CDS             complement(2035..3051)
                     /gene="aroF"
                     /locus_tag="VFI43_00905"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7060718.1"
                     /translation="MIIVMASGATNEQIDAVVDRIQGFGLDANISRGTERIVIGAIGD
                     EQKLDPEMFDALAGVEYSMHVVKQYKIVSRESHKHDSIINIGETQIGGNQVQVIGGPC
                     SVETQEQMDLAAEQVSGAGCTLMRGGAFKPRTSPYTFQGNGEEGLKMFRRAADKYNLG
                     IVTELMDARMLDTFLEYDVDVIQIGTRSMQNFELLKEVGRTNKPVILKRGMSATVSEW
                     LMAAEYIAAGGNHNIIFCERGIRTFETAYRNVMDVTCIPVLKKETHLPVIVDPSHAGG
                     KTWMVPALARAAVAAGADGLLVEMHPNPCEAWCDADQALDPAEFQSLMESLKGIAQAI
                     GRTL"
     gene            complement(3048..4235)
                     /gene="hisC"
                     /locus_tag="VFI43_00910"
     CDS             complement(3048..4235)
                     /gene="hisC"
                     /locus_tag="VFI43_00910"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7060719.1"
                     /translation="MNLCELAPEYIRAISPYQPGKPISELAREMGIDQRFIIKLASNE
                     NPLGTSPLALEAMNHALSEVALYPDGSGYELKTALSQRYAVTRDQIVLGNGSNDVLEL
                     AARVFLKPGASAVYSQHAFAVYPLVTQAVGASGISVPARDYGHNLDAMLDAVTPETRM
                     VFIANPNNPTGTLLPGSAVLRFLERASPDVLVVLDEAYNEYLPASVKADSISWLKRFP
                     NLLITRTFSKAYGMAGVRVGFGLAHEEVSNLMNRVRQPFNVNSIGLAGAVAALEDVEF
                     VKRSYALNQAGMLQITTGLRQLGIEYIPSYGNFLSFRVPGDAKAVNQSLLEQGVIVRP
                     IGIYEMPQHLRVTIGLESENAKFLKSLEIAVGKLSGDQPEELMPQVSKAAGTAKMAAG
                     ESA"
     gene            complement(4292..5359)
                     /gene="pheA"
                     /locus_tag="VFI43_00915"
     CDS             complement(4292..5359)
                     /gene="pheA"
                     /locus_tag="VFI43_00915"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7060720.1"
                     /translation="MMDQLKHLRDKIDVIDSELLRLISARAGLAREIGQIKNGVAYRP
                     EREAQVLSRLNALNEGPLANEHIGRIFTEIMSLCRSMEEPLSVAYLGPQGTFSEEAAL
                     KRFGSVVTALPCSSIDDVFRKVESGSAGYGVVPVENSTEGAVGRTLDLLLQTPLKVCG
                     EVALPIHQFLLARHPDVSRIKKIYSHPQSFSQCHEWLNLNLPHLTGDARINAASNADA
                     ARLAAEDENAAAVAGKKAGEVYGLAVCARNIEDDPNNTTRFLVIGAQEVGPSGKDKTS
                     MVMSTQNRPGAIHELLAPLAQNGVSMSRLESRPSRAGLWEYVFFVDIEGHQQDSKVSQ
                     ALREVTAKAAFLKVLGSYPAV"
     gene            complement(5414..6613)
                     /locus_tag="VFI43_00920"
     CDS             complement(5414..6613)
                     /locus_tag="VFI43_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HET7060721.1"
                     /translation="MPERAHKTFRILTLNQISPLGLSLFSPSRYVVGDDVADPDAILV
                     RSHNMLQMEIPRSVTAIGRAGAGTNNVPVKDMNLRGVPVFNAPGANANAVKELVLAGL
                     LIASRNLIPAIHFTENLQGDDAALNKQAEDNKKKFSGIELPGRTLGIIGLGAIGRLVA
                     EAALRLGMKVTGYDPNITVDAAWNLPSEVKRAESIQDLIRNNEFVTLHIPLLDSTRHL
                     IDHEVVQQMKKGTILLNFSREGIVDENAVLEGIESGKIKYYVCDFPSRIFQHHPNVIT
                     LPHLGASTLEAEENCAVMVVKQLIDYLENGNIKNAVNFPNLTIERESPYRLAVANANV
                     PNMVGQVSTSMARAGLNIHNMGNKSRGEMAYTLVDVDSPVPQKALEEIAEIKGVLMVR
                     YLPIPAK"
     gene            complement(6606..7700)
                     /gene="serC"
                     /locus_tag="VFI43_00925"
     CDS             complement(6606..7700)
                     /gene="serC"
                     /locus_tag="VFI43_00925"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET7060722.1"
                     /translation="MKHIYNFSAGPAVLPVEVLRQARDEMLDWHGSGMSVMEMSHRGK
                     EFMSIFAKTEADLRELAGIPDNYKILFLSGGASSQFAMVPMNLLRGKKSANYINTGEW
                     SKKAIKEAKRYCAVTVAASSEDKNFTYVPPQDTWNIAPDAAYLHYTPNETIGGVEFNW
                     IPDLTRMGTDMPLVADMSSSFMSRPLDVSRFGLIYAGAQKNVGPAGLCIVIVREDLLR
                     TTLPGTPTMFDYKIQADNDSMYNTPPTYPIYICGLVLEWLKQKGGLVEMEKTNIAKAN
                     LLYDFLDSTDFYHCPVVKADRSRMNVPFTLENTRLDEEFLKQAKEHGLIQLKGHRSVG
                     GMRASIYNAMPLEGVEALVEFMREFASKHA"
BASE COUNT         1735 a         2149 c         2045 g         1877 t
ORIGIN      
        1 ttattgttag atcggaaaca atgaatatat ttgcccgacg ctctcatggg tttgccgccg
       61 attgtttacc atgtctccat tgctagcgaa catggcacgg gcaaaaagcg gtctaggtgt
      121 aacagagcca acagttcagg atcaatatat aggcttcgat cttcccttta tctattcttt
      181 tcggcattct gccttggaac aaacgagagg tttttgtctt ctccagcgtt cttataaaac
      241 gctgggtcat ctgtggctag acgataatcc cacccggaat tagctttctc tgtgcgattc
      301 ctcacatacg catttctcat aaactgctat atccacgcca tgctcaatat caaaaatcac
      361 aatacccttg aagctcttga tcgtcgtatc ggcaacatga ggcaaaacat cgagggcttc
      421 aagctggata ctgactgcgt gcatgcggtt atcaggaata tcagatggaa gaaataaagg
      481 agcgcttcaa ggaggattct tacgcatggc atttataaga atgtgcaagc agcttgccgc
      541 gaatcgggaa tccctgcaaa atgcccctgc aaatgcattg gcagatcgaa caaatatttt
      601 tgaataacgt tcagggagga acacgatggc tagcgttgga taccacgagc cgattgagga
      661 gctatccgat gaaaccaggg atatgcacag ggcaatcaca tcgctgatgg aggaactgga
      721 ggcggttgat tggtacaacc agcgcatcag tgcatcgaaa gatgcccagc tcaaggcgat
      781 tcttacgcat aatcgcgacg aagagaaaga acatgcgtcg atgctgctcg agtggatccg
      841 gcgcagggac catgtatttt cgaatgaact gaaagaatac ctgtttaacg agaacccgat
      901 cgttcacgaa caaaaaacgt aaccgggcta gcagcctggc ttaggcttgc ctaaaagaga
      961 tcaccggcag agattacggc cgagtattcc ggccggaagg cgcagtgagg gattatttgg
     1021 tggcgttatt ggagttcggc ttcgataagc acgggcagtt caggacaggc tttcgagcca
     1081 atttcgccga acttggcgag cgtcgctaaa aacctgtttc agcgaattgc tgtccccgcg
     1141 tgcaagcatt tccctgattt cggcaagttc gtgctgatac gcgtcgattt cattgcagag
     1201 ggcatcctgg ttggctaaac atatgtcccg ccacatttca ggcgagcttt ccgcgatgcg
     1261 ggtaaaatca cgaaatccgc ttccggcaaa acccagcgca tcgcctgggc caagcgcctc
     1321 atcaacacct gattcatccc tggaatgaag gttcgaggcg tagaaccggt gcataagcgc
     1381 gaatgcaagc acgtgaggaa gatggctcac cgacgcaaga acttcatcat gccgggcggc
     1441 attcattagc gagacgcgtg cgccgcatgc ctgccatagc tcgattacac gtttggtcgc
     1501 actttcgctg gtttcatccc ccggcgtcaa tatgacgttt tttccccgga acaagccggg
     1561 acgtgcggct cccgccccag attgttccgc tcccgcgaca gggtgaccgg gaacaaacat
     1621 tgccagatga ctggccagat ggttttttgc cgcgataatc acattttgct tcgtactacc
     1681 agcgtcggta acgattgttc cggactgcag gtgaggagcg atttcgctca tgatcgcgcc
     1741 ggtttgcccg accgggacag atagaaatac aatttccgca tttttcaatg ctgattggaa
     1801 gtcagcgccg atttcatcga tagcccctaa tttcagcgcg cgctgaacat tctccgggct
     1861 acggcccata cccactatat actggacctc gccggcctgg cgaagcgcga gtgcaagcga
     1921 gccgccgatc aggccgactc cgataatcac cagtttgctg atcatgtgat cgtcattaca
     1981 gggtttgctc aaggtttcgt tttagaatcg cgttctacag ggttcggttc ggttctacaa
     2041 ggttcttccg atagcttgcg ctattccctt cagtgattcc atgagggact gaaattcggc
     2101 cggatcgagc gcctgatccg cgtcacacca cgcctcgcat ggattgggat gcatttccac
     2161 gagcaatccg tcggccccgg cagccaccgc cgcccgtgcc agagccggaa ccatccaggt
     2221 tttacctcct gcgtgagaag ggtccacgat caccggcaga tgcgtttctt tttttaacac
     2281 cgggatgcat gtcacatcca ttacattccg gtaggcggtt tcgaaagtac gtatgccccg
     2341 ctcgcaaaaa ataatgttgt gattgccgcc tgccgcgatg tattctgccg ccatcagcca
     2401 ctcggaaacg gttgcggaca tgccgcgctt gagtattacg ggcttgttgg tgcgccccac
     2461 ctctttcagc aattcgaagt tctgcatgct gcgcgtgccg atctggatca catccacgtc
     2521 atattcgaga aatgtatcaa gcatgcgggc gtccatcagt tcggtcacga tgccaagatt
     2581 gtatttgtcc gctgccctgc ggaacatttt cagcccttcc tcgccatttc cctgaaaggt
     2641 atagggactc gtccgaggct tgaaagcccc tccgcgcatc agcgtgcagc cggcgccgga
     2701 aacctgttcc gcagccagat ccatttgctc ctgcgtttct acagaacagg ggccgccgat
     2761 aacctgcacc tggttcccgc caatctgcgt ttccccgata ttgatgattg aatcatgctt
     2821 gtgcgattcg cgcgaaacga tcttgtattg cttgacgacg tgcattgaat actcgacccc
     2881 ggccaatgcg tcgaacattt cgggatcaag tttttgctca tcaccaatag cgcctatgac
     2941 gatacgctct gtaccgcgag agatattcgc gtccagaccg aagccctgaa tccgatctac
     3001 gactgcgtca atctgctcat tggtggcgcc gctggccatc acgataatca agcggattct
     3061 cctgctgcca tcttggcggt tccagcggct tttgaaacct gcggcatcaa ttcctccggc
     3121 tggtcaccgg agagcttccc cacggcgatt tccagcgact taaggaattt cgcattctcg
     3181 gattcgaggc cgatggtaac cctgagatgt tgaggcattt cgtaaatacc gatggggcga
     3241 acaatgaccc cttgctcaag gaggctttga ttcacggcct tggcgtctcc cggcacccgg
     3301 aaactgagga aattaccgta ggaggggatg tattcgatgc ccagctgccg caaaccagtg
     3361 gtgatctgga gcatacctgc ctggttgaga gcatacgatc gtttgacaaa ttccacatct
     3421 tccaatgcag caactgcacc ggcaaggcct atgctattga cgttgaacgg ctggcgcaca
     3481 cggttcatca aattactcac ctcttcatga gcgaggccaa agccgacccg aacgcctgcc
     3541 atgccgtagg ccttggaaaa agtccgcgtg atcagaagat tcgggaaacg ttttagccag
     3601 gaaatgctgt ctgcctttac cgaagcaggc aagtattcgt tatatgcctc atccagcacc
     3661 accagcacgt cgggcgacgc cctttcgaga aagcgcagca cggctgagcc cgggaggagc
     3721 gttcccgtag gattgttcgg gttcgcgata aataccattc gtgtctcggg cgtgacggca
     3781 tccagcatgg catccagatt gtggccatag tcccgcgcag gcacagagat tccgcttgcg
     3841 ccaactgcct gggtgacaag gggataaaca gcgaatgcgt gctgcgagta aaccgcggag
     3901 gcgccgggct tgaggaatac gcgcgccgcc agttccaaaa cgtcattgga cccattcccg
     3961 agcacaatct gatcccttgt cactgcatag cgttgcgaca gagccgtctt cagttcgtaa
     4021 ccgcttccgt cgggataaag cgccacctca ctgagcgcat gattcatcgc ttccagcgca
     4081 agcggactcg taccaagcgg gttttcgttc gatgccagct tgatgatgaa gcgttggtcg
     4141 atacccattt ccctggccag ttcagagatg ggtttgcctg gctggtaagg actgatagcg
     4201 cggatgtatt cgggggcaag ctcgcaaagg ttcataaggt catatccatg aattgctttt
     4261 ttagaaaaag gatggctgaa agcggcaggc gtcataccgc ggggtaagag ccgagcactt
     4321 tcaggaatgc cgcttttgcc gttacttccc gaagtgcctg cgatactttt gaatcctgct
     4381 gatggccttc gatatccacg aagaaaacat actcccaaag gcctgcacgg gaagggcggg
     4441 actccagccg gctcatgctt accccatttt gggccaaggg ggccagcagt tcgtgtatcg
     4501 cgccggggcg gttttgagtc gacatgacca tcgacgtttt atccttgccc gatggtccca
     4561 cctcctgcgc gccaatcacc agaaagcgag tggtattgtt gggatcatcc tcgatattcc
     4621 tggcgcaaac cgcgagccca tacacctctc ccgctttttt tccggctacc gcggcggcat
     4681 tctcatcctc tgctgcaagc cttgcagcgt cggcattgct tgcggcatta atgcgcgcat
     4741 cgccagtcaa atggggcaga ttgaggttaa gccactcgtg gcattgagag aatgactgtg
     4801 gatgagaata aatctttttt attcgcgaaa catcaggatg gcgagccagc aaaaactgat
     4861 ggatcggcag cgcaacttcg ccgcatacct ttagaggggt ttgaagcaac aggtcgagcg
     4921 tccttccgac cgctccctcc gtggaattct cgacggggac gactccgtac cccgcgctgc
     4981 ctgactccac ttttcgaaat acatcgtcaa tggaactgca gggtaatgcc gtaacgacac
     5041 tgccaaagcg cttcaatgcc gcttcttccg aaaacgtccc ttgcgggcct aggtacgcga
     5101 cagacaaagg ttcttccatg gatcggcaaa gtgacatgat ttctgtaaat atccgcccga
     5161 tatgctcgtt tgccagcggc ccctcattca gcgcattcaa ccgggagagt acctgggctt
     5221 cccgctccgg gcgatatgca accccattct tgatctgccc gatttcacgc gcaagacccg
     5281 cgcgcgcact tattaacctc aatagttcac tgtcaatgac atctatcttg tcgcgcagat
     5341 gtttgagctg atccatcatc aagggattgt gctgattctc gggggctggg cgaatttcat
     5401 cggccagtcc tgcttatttc gcaggtatag gcaggtagcg caccatcagc acgcctttga
     5461 tttccgcaat ctcttccagc gccttttgcg gtacgggact gtccacgtca acaagcgtgt
     5521 aggccatctc gccccgggac ttgttgccca tgttgtgaat attgagacct gctctcgcca
     5581 tgctggtcga tacctgtccg accatgttgg ggacgttggc gttggcaacg gccaggcggt
     5641 aaggagattc gcgctcgatc gtaaggttcg gaaaattcac cgcgtttttg atattgccat
     5701 tttcaaggta atcgatgagt tgcttcacca ccatgacggc gcagttctct tcagcttcca
     5761 gggtcgaagc gcccaggtgc ggcagtgtaa tgacgttcgg gtgatgctgg aatatgcggc
     5821 tggggaaatc gcagacgtaa tatttaatct tgccggattc gattccttcg agcacagcgt
     5881 tctcgtcaac aatgccctcc cgggagaaat tgagcaggat cgtgcctttt ttcatctgct
     5941 gcacgacttc gtgatcaatc aggtgtcgcg tcgagtcgag cagcggtatg tgcaacgtca
     6001 cgaactcatt gttgcgtatc aggtcctgga tgctctccgc cctttttact tccgatggca
     6061 gattccaggc agcatccacg gttatgttag ggtcatagcc agtcactttc atgccgagcc
     6121 ggagtgccgc ttcggcgacc agccgcccta tcgcgcccag gcctattatt cccagggttc
     6181 ttcccggtaa ttcgatcccc gaaaactttt tcttgttgtc ttccgcctgc ttgttgagcg
     6241 cagcgtcatc cccctgcaga ttttcagtga agtgaatggc tgggatcagg ttgcgcgacg
     6301 caatcagcag gcccgccagc accagttctt tcacggcgtt tgcgtttgcg cccggggcat
     6361 tgaatacagg aacgccgcgc aggttcatgt cttttacggg aacgttgttg gtgcccgctc
     6421 ctgcccggcc tatcgctgtc acgctgcggg gaatttccat ttgcagcatg ttgtgggaac
     6481 gcacaaggat cgcgtccggg tcggcaacat catcgccgac gacgtaccgg gatggggaaa
     6541 aaaggctcag gcccagcgga gaaatctggt tgagcgtcag tatccgaaag gttttatgcg
     6601 cgcgttcagg catgcttgct tgcaaactcc ctcatgaatt ccaccagcgc ttctactcct
     6661 tcgagtggca ttgcgttata gattgaagcg cgcattccgc caaccgaacg gtgccctttc
     6721 agctggatca ggccatgctc cttcgcctgc ttgagaaatt cctcatccag gcgggtattc
     6781 tccagcgtaa acggtacatt catgcgcgac cgatccgcct tgaccaccgg gcagtgatag
     6841 aaatcagtcg aatcgagaaa atcgtacaac aggttggcct tggcgatatt ggttttctcc
     6901 atctcgacca atccgccttt ttgcttcagc cactcgagca caagtccgca aatatatatc
     6961 ggatacgtgg gcggcgtgtt gtacatcgaa tcgttatcag cctgaatctt atagtcgaac
     7021 atggtcggcg tgccagggag ggttgttcgc agcaagtctt cccgtacgat gacaatacat
     7081 agccccgccg ggccaacatt cttttgcgca cccgcgtaaa tcaatccgaa acggctaaca
     7141 tccagagggc gtgacataaa gctggaggac atgtccgcga ccaggggcat atcggtaccc
     7201 atccgggtca ggtcgggtat ccagttgaat tccacgccgc cgatggtctc gttcggtgtg
     7261 taatgcaaat aagcggcgtc cggcgctata ttccaggtgt cctgcggcgg cacgtaggtg
     7321 aaattcttat cctcggacga agccgcaacc gttaccgcgc agtatcgttt ggcttccttg
     7381 atggcttttt tggaccattc acctgtattg atatagttcg cgcttttctt gccgcgcagc
     7441 agattcatgg gaaccatcgc gaactggctg gaggctccgc cggagagaaa aagaatcttg
     7501 taattatcgg gtattcccgc caactctctc agatcggctt ctgtctttgc gaagattgac
     7561 atgaactcct tgccgcggtg gctcatctcc atgaccgaca tgccgctccc gtgccagtcc
     7621 agcatctcat cgcgcgcctg ccgcaatacc tcaaccggaa gtaccgctgg ccccgcgctg
     7681 aagttataga tgtgtttcat tggaattttt taccttctgc aattcggcca cagaaaaatg
     7741 caaagaagag taagagatag gggacaccgg catcaggaac atgggccagg aaatctcttt
     7801 agtagc
//
LOCUS       DASSHJ010000015         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_100672, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000015 DASSHJ010000000
VERSION     DASSHJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100672"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFI43_00930"
     CDS             complement(<1..893)
                     /locus_tag="VFI43_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7060723.1"
                     /translation="MSDITKASQRFSYPFATLQQIAHDVLDHAKKGGASGCETTIDDG
                     FGQNVTVRRGEVETIEYNRDKGISVTVYIGQKRGHASTSDFSPQALRETVAAALSIAG
                     HTAEDSCAGLADRDLLAQEFPDLELYFPWDLPVEQAIELAKKCEAAAFAVDERITNSE
                     GASVHLSESQFIYANSLGFMAGYPASRHSISCAVIASEKDAMQRDYWYSTARDPSDLE
                     AAHSVGNQAGVRSVRRLGARKINTCEVPVLFEAPVASSLIGHFVSAVSGGNLYRRSSF
                     LVDSIGRQVFAPDIQIRELPH"
     gene            1118..1645
                     /gene="yjgA"
                     /locus_tag="VFI43_00935"
     CDS             1118..1645
                     /gene="yjgA"
                     /locus_tag="VFI43_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HET7060724.1"
                     /translation="MDKKPEDDREEHLPPSKTRQKKEMHARQDIGERLAQLDPKSLST
                     FDLPEGLADAINDARSMRAHGARRRQMQLIGKLMREVDPVPIQKKLDALNRPGVQQTA
                     RLHLLERWRDRLLADEKGLMDFAQAYPAADLQHLGTLVRDAKGERLANKPPRQFRELF
                     QELQRIVPENPGTKT"
     gene            complement(1635..2732)
                     /locus_tag="VFI43_00940"
     CDS             complement(1635..2732)
                     /locus_tag="VFI43_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET7060725.1"
                     /translation="MKRREFIKLAGVTAALIPVAPSVFGEQIIPRRPHSYRLTYQVDL
                     PGDGKKARLWLPLPDADDTPYQFTQGNIWKGNPDVARFENIPGTASPMFYAEWRHGGP
                     RTVTVSSIVKTTDRAVDLLTYKEQAGAAIPADVKHYLHATKHIPLDGIVRKTALSIVK
                     DANAQTPLQKAHAIYNWVVDKSYRDPNARGCGRGDIKSMLESGNLGGKCADLNTLFVG
                     LARAVGVPARDNYGIRIDESASHKTLGQAGDVTTAQHCRPEFYLAGLGWVPVDPADVR
                     QLVLDEGLPIGNARVVALRNKLFGSWEMNWVAFNHARDVKLAKDSVIGELPFFMYPEA
                     EVGGHVRDSLEPQDFAYKIISAELVGTGVKF"
     gene            2917..3606
                     /locus_tag="VFI43_00945"
     CDS             2917..3606
                     /locus_tag="VFI43_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7060726.1"
                     /translation="MTPASPALLPSVELKTGSAPAHTVIWLHGLGADGNDFVPIVKQL
                     VPTLNIPPVRFLFPHAPIRPMSINRGMMMRAWYDYDLLDPNLGLRENVPSLIASQQAI
                     EALIAREVEGGVSPQCIVLAGFSQGGALALHAGLRYPEKLAGIMALSAYLPAPATLHA
                     EGSRENLATSVFMAHGINDHVVPMILAETSRRQLIESGYPLEWHEYRMAHSVCDEELA
                     DISAWLNKVLQ"
     gene            3874..>4096
                     /locus_tag="VFI43_00950"
     CDS             3874..>4096
                     /locus_tag="VFI43_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060727.1"
                     /translation="MNTPLTPLQNLAAPAFPGQSSDLMTERNSTRTEHAGQRIAHIDD
                     DPDIRDTVKRILHVNGYAVESYQTMRDFTT"
BASE COUNT          943 a         1134 c         1090 g          929 t
ORIGIN      
        1 aaatgcggca gctctcggat ttgaatgtcg ggtgcaaata cctggcgacc gatgctgtcc
       61 acgaggaagg aggatcgcct gtagagattt cctccgctca ctgcgcttac aaagtgcccg
      121 ataagactcg aggcaacggg cgcttcaaac agtacgggca cttcgcaagt attgatttta
      181 cgtgcaccca gtcgcctgac actgcgcacc ccggcctggt tgcctacgct atgagccgcc
      241 tccagatcag agggatctcg agccgtcgag taccagtagt cgcgttgcat cgcgtccttc
      301 tcgctcgcaa tgacagcaca actgatactg tgccgcgagg caggatagcc ggccatgaat
      361 ccgaggctgt ttgcatatat gaattgtgat tcgctcaaat ggacgcttgc tccttccgag
      421 ttggtaatgc gctcgtccac tgcaaacgcc gccgcttcgc atttttttgc aagctcgatt
      481 gcctgctcca ccggcaaatc ccacgggaaa tacagctcga gatccggaaa ttcctgcgca
      541 agcaaatcgc ggtcggccaa gcccgcgcaa ctatcttctg ctgtatgacc cgcgatcgat
      601 agagcggcag ctaccgtttc tcgcagagcc tggggagaaa aatcggacgt gcttgcatga
      661 ccgcgctttt ggccgatgta gaccgtgacc gatattcctt tgtcgcgatt gtactcgatg
      721 gtctccactt cgccccgccg gactgtgaca ttctgaccaa acccgtcgtc aatggtcgtc
      781 tcgcagccgc tggcgcctcc ttttttcgca tggtcgagaa cgtcatgtgc gatttgttga
      841 agtgtcgcga aaggatagga aaatcgctgc gatgccttgg taatatcact catggaagat
      901 caagggttgc gaggttatgg aaccagcttc aagcaagcgt gcaatcagaa cgattcacgt
      961 tatctatatg caggcgtctc gggatgaaaa cattgaacgg cgttatgata acaggttttg
     1021 gaggggtaaa ttgacgaggc ggaaagagtc ctggcaatgg ggatttgaag ctttatgaaa
     1081 agctctgaca agatttatga ggaatatgaa ggatattatg gataaaaaac cggaagacga
     1141 tcgggaagag catctcccgc ccagcaagac gcgccagaaa aaggaaatgc atgcacggca
     1201 ggatatcggc gaacggctgg ctcagctcga tccaaagagt ctatccacgt tcgatctgcc
     1261 ggagggactg gcggatgcaa tcaatgacgc gaggagcatg cgagcgcacg gggcgcgacg
     1321 ccgccaaatg caattgatcg gcaaattgat gcgcgaggtt gaccctgtgc cgatacagaa
     1381 aaagctcgac gcgttgaacc ggccaggcgt gcagcaaacc gcaaggctgc atttgcttga
     1441 acgatggcgt gatcgactac tggcggacga aaaggggctc atggactttg cccaggccta
     1501 tcctgccgcc gatctgcagc acctaggcac cctggttcgc gatgcgaagg gggagcggct
     1561 tgcgaacaag ccgcccaggc agttccgtga attatttcag gaactgcaaa ggatcgttcc
     1621 ggaaaacccc ggcactaaaa cttgaccccc gtgccaacca gctctgctga aataatcttg
     1681 taggcaaagt cttgcggttc gagactatcg cgcacgtgcc ccccgacttc cgcctcgggg
     1741 tacataaaga atggcagttc gccaatcaca ctgtccttcg ccagtttaac gtctcgagca
     1801 tgattgaacg ccacccagtt catttcccag gaaccgaata atttattgcg aagcgccacc
     1861 actctcgcgt tgccaatagg caatccttcg tccaagacta gctgacgtac gtccgccgga
     1921 tcgacaggta cccagcccag gccggcgaga tagaactcag ggcggcagtg ttgcgcggtt
     1981 gttacatccc ccgcctgccc gagcgtcttg tggctcgccg attcgtcgat acggattcca
     2041 tagttatcgc gcgccggcac acccactgcc cgggccaacc ctacgaaaag ggtattcaga
     2101 tccgcgcatt tgccccccaa gttaccgctt tcgagcatcg atttgatgtc tccacgcccg
     2161 caaccgcgtg cattcgggtc gcggtaggat ttgtcgacta cccaattgta tatggcatgc
     2221 gccttctgga gcggcgtttg cgcatttgca tcctttacaa tggagagtgc agtcttgcgt
     2281 acgatgccgt cgagcggtat atgtttggtc gcatgcagat aatgttttac atcagctgga
     2341 atggccgctc ctgcctgctc cttataggtg agcagatcca cggcgcggtc tgtcgtcttg
     2401 acaatgctgc ttacggtaac ggtgcgcggg ccgccatggc gccattctgc ataaaacatg
     2461 ggggaagcag tgccgggaat gttttcaaac ctcgcaacgt ccggattgcc cttccatata
     2521 tttccctggg taaactggta aggagtgtcg tcggcatccg gcagaggcag ccataaacgc
     2581 gccttctttc catctccggg cagatcgacc tgataagtca agcgatagct gtgcgggcgc
     2641 ctcggtatga tctgctcacc aaataccgat ggcgcaacgg gaatcagggc cgcggttacc
     2701 ccggccagtt tgatgaactc cctgcgtttc atgatcttct cctgatggat cgattaatgt
     2761 aaaaaattgc aagtgcggaa tctaatcccg cgttccggtt aagtcaaggg agtatcaacg
     2821 cttgctttta catccctcga ttggcaaacc tggcgccagg aagtcaaccc aaagctgata
     2881 aaatccaggt ttctcccacc ggttgttttt gcatccatga cccctgcctc gcctgcgttg
     2941 cttccctctg tggaactcaa aaccggttcc gcacctgctc ataccgttat atggctacat
     3001 ggcctgggtg cggatggaaa tgacttcgtt cccatcgtaa agcagctcgt cccaactctg
     3061 aatatacccc ccgtgcgttt tctttttccc cacgccccga tccggccgat gagcatcaat
     3121 cggggaatga tgatgcgcgc gtggtatgac tacgacctgc tcgatccgaa tttgggcctt
     3181 cgtgaaaacg tgccgagcct gattgcatca cagcaggcaa ttgaagcgct catagcgcgg
     3241 gaagtggaag gaggcgtttc gccccagtgt atcgttctgg caggtttttc ccagggaggc
     3301 gccctggcgc ttcatgcggg attgcgttat cccgaaaaac ttgccggcat catggcgctt
     3361 tccgcctatc tgcctgcccc cgctacactc catgccgagg gcagtcggga aaaccttgcg
     3421 acgtcagttt tcatggcgca tggcattaat gaccatgtag tgcccatgat ccttgcggag
     3481 acctcgaggc gacaacttat cgaatcgggt tatccactcg aatggcacga atatcggatg
     3541 gcgcatagcg tctgtgacga agaattagcc gatatcagcg cctggctgaa caaggtcttg
     3601 cagtagccag acggtctcaa ggagagctag cccaagatgc ggagccacgc acaggtcttc
     3661 gctattggtc gcgcctgaag tttggtatta cccatcgcaa ccatctgatc gaatcggttt
     3721 cgcttcacga aatcttaaac ttctcctgac ggttccttca caacgcctgc attaccctgc
     3781 ttgaaacagg gtaattatgt gctatatttc ccttatggca ccatggaaat ttttacagca
     3841 ggggcgattt gataacttat agaaggagga aaaatgaata cccccctcac cccgcttcag
     3901 aaccttgcgg cacccgcgtt tccgggtcaa tcatctgatc tcatgacgga aaggaacagc
     3961 acccgaaccg aacacgcggg ccagcgaatc gcacatattg atgacgatcc ggatattcgc
     4021 gacacggtaa aacgcatatt gcacgtcaat ggttatgcgg tcgagagcta ccagacaatg
     4081 agggatttca cgactt
//
LOCUS       DASSHJ010000016         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1189599,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000016 DASSHJ010000000
VERSION     DASSHJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189599"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..178
                     /locus_tag="VFI43_00955"
     CDS             5..178
                     /locus_tag="VFI43_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET7060728.1"
                     /translation="MDTARGYISWISPMARALIKAREGDVVILNAPGGLQELEILEVK
                     YQTIEMEPFVGEE"
     gene            262..1068
                     /locus_tag="VFI43_00960"
     CDS             262..1068
                     /locus_tag="VFI43_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7060729.1"
                     /translation="MKFITSRHNAFFKQLMEVGESAQERKDLGLTLLDGAHLIEAYRQ
                     ALGQPTRLIVSETGWETSEIKCLLAEKGAADKEVVVLTDNLFQVASPVKTPTGIMALI
                     SIPRPENIPLRAAQRDESFSVLLEAMQNPGNLGSILRSAAAASASHVYLSEGCADVWS
                     PVTLRAAMGAHFLLRIYEQSDIANIAKTFNGKVVATSLKAKTSLYQTALTGPIAFVFG
                     NEGAGLSDEVLAATNEQIIIPMPGGTESLNAASAAAVCFFERVRQMKANS"
     gene            complement(1128..2159)
                     /locus_tag="VFI43_00965"
     CDS             complement(1128..2159)
                     /locus_tag="VFI43_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7060730.1"
                     /translation="MNNGAPPPDASRPKAKGEPLPGTIPVRRPVPHITDYEHGISAID
                     ADYLRPGLAAIHLVVEGGKAALVDTGTSSGAGGLIEVLKGRNLGPEDVLYVFVTHIHL
                     DHAGGAGELMHHFPNARLVVHPRGARHMADPARLVASAMSVYGEDQFRRMYGEIRPVD
                     ASRIIEAPDGFRLDFNGRPLLFLDTPGHARHHYCIFDSRSQSFFTGDTFGISYREFDV
                     DGMEFVFPTTSPVQFDPSAAHASLDKLMGFNPIHAFLTHYGRVGHLPQHAAELHELVD
                     AHVDIARGLRNAPDRHRAMVEKLGNLLLNRIIAHGCKLPEGEIRDLLSLDVGLNVQGL
                     ENWLDRSLP"
     gene            complement(2175..>3741)
                     /gene="acs"
                     /locus_tag="VFI43_00970"
     CDS             complement(2175..>3741)
                     /gene="acs"
                     /locus_tag="VFI43_00970"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET7060731.1"
                     /translation="RKGDRVIIYMPMSIEAVVAMQACARIGATHSVVFGGFSAKSLHE
                     RITDAGAVAVITADEQHRGGKINHLKAAVAEALAMGGTDSVKSVIVYKRGEGNLGLNS
                     PRDVWWHELIQGQSDGCEPEWVNAEHPLFTLYTSGSTGKPKGVQHSTGGYLLGTIVSM
                     LWVFDYKPTDIFWCTADVGWVTGHSYVAYGPLAVGATQVVFEGVPTHPDAGRFWKMIQ
                     NHKVTTFYTAPTAIRSLIKLGSDLPGQYDLSSLRLLGSVGEPINPEAWMWYYTAVGQS
                     RCPVVDTWWQTETGSHMIAPLPGAIPLKPGSCTLPLPGIKAAIVDEAGHDVPDGKGGF
                     LVIKRPFPSLARTLWGDPERFKKTYFPEELGGGYYLAGDSANRDLDGYFWIMGRIDDV
                     LNVSGHRLGTMEIESALVASPLVAEAAVVGKPDEIKGEAIVAFVVLKGSRVQGSDAQK
                     TAIELRNWVSGEIGPIARPDEIRFGDNLPKTRSGKIMRRLLRTLARGEEITQDVSTLE
                     NPAILEQLKQPIA"
BASE COUNT          800 a         1037 c         1088 g          816 t
ORIGIN      
        1 tgaaatcgat acggccaggg gttacatcag ctggatttcg ccgatggcgc gcgcactgat
       61 caaggcgcgg gaaggcgatg tggttatatt gaatgcgcca ggaggacttc aggaactcga
      121 gatactggag gttaaatacc agacgatcga gatggagcca tttgtcgggg aagagtgagt
      181 gcatggggtt attgcaggtc tgtcggcagg aaacttcatg tggctgagaa ttgaagctcc
      241 cgggagagga ggtgttctcc gatgaaattt atcacttctc gccataacgc atttttcaag
      301 caattgatgg aggtgggcga gtctgcgcaa gaacgcaagg accttggatt gacgcttctt
      361 gacggcgccc atctgatcga ggcctacagg caggcgctgg ggcagccgac acgactgatc
      421 gtcagtgaga ccggttggga aaccagcgag ataaagtgct tgctggcgga gaagggcgcg
      481 gcagataagg aagtcgttgt gttgacggac aatctgtttc aggtcgcttc tccggttaag
      541 acgcccaccg gcatcatggc cctcatatcc attcccagac ctgaaaatat tcctctacgg
      601 gctgctcaaa gggacgagtc tttttcggta ttgctcgaag cgatgcagaa tcccggcaac
      661 ctcggttcaa tccttcgctc cgctgctgcc gcaagcgcaa gtcatgtgta tctttcggag
      721 ggttgcgcgg atgtctggtc ccctgtaacg ctgcgcgcgg caatgggtgc tcactttttg
      781 ctgcgcatat acgagcagtc cgatattgca aatattgcga agaccttcaa tggcaaggtg
      841 gtcgcgactt cgttgaaggc gaaaacgagt ctctaccaga cggccttgac cggacctatc
      901 gcattcgttt tcggcaacga aggcgcaggc ctatcggatg aggtattggc ggcgacgaac
      961 gaacaaatta ttatccctat gccaggtggt acggaatccc tgaatgcagc atcagccgca
     1021 gcggtatgtt tttttgagag agtacggcag atgaaagcga actcgtaggc cgtgtcatgg
     1081 cacagcacct gctacccctg atgctgcttg tgttgcattg cagagtgtta gggaagcgag
     1141 cggtccagcc agttctccaa tccctggaca ttcagtccca catccaggct cagcaggtcg
     1201 cggatttctc cttccggcaa cttgcagccg tgcgcaatga tcctgtttag caggagattt
     1261 ccaagtttct ccaccatggc cctgtgcctg tccggcgcgt tgcgcaatcc cctggcaata
     1321 tcgacgtgcg cgtcgaccag ttcgtgcagt tcggcagcgt gttggggcag gtgccccacc
     1381 cgcccgtaat gggtcaggaa tgcatggatg gggttaaagc ccatgagctt gtccagtgag
     1441 gcatgcgccg ctgacgggtc gaactggacc ggacttgtgg tagggaacac gaactccatc
     1501 ccgtccacat cgaattcccg gtaggaaatg ccgaaagtat cgccggtaaa gaacgactgg
     1561 ctgcggctgt cgaaaataca ataatggtgg cgggcgtggc caggcgtatc aaggaaaagc
     1621 agcggcctcc cattgaaatc cagcctgaat ccgtctggcg cctcgataat ccggctggca
     1681 tcgaccggcc ggatttcccc atacatgcgc ctgaattgat cctccccgta gactgacatc
     1741 gcactggcta ccagcctggc tggatcagcc atgtggcggg ctcctctagg gtgcactaca
     1801 agcctagcat tggggaaatg atgcatgagt tcaccggctc cgcccgcgtg atcgagatga
     1861 atgtgcgtga cgaacacata taagacatcc tcgggaccga ggtttctgcc tttcagtact
     1921 tcgatgagcc caccggcccc ggacgaggtg ccggtatcca ccagggcggc cttgccgcct
     1981 tcaacaacca gatggattgc cgcgagaccc gggcggagat aatctgcgtc aatggcactg
     2041 attccatgct cgtaatcagt aatgtgtggg acagggcgcc gcaccggaat ggttccgggt
     2101 aaaggttcac cctttgcttt cgggcgggaa gcgtctggcg ggggagcccc gttgttcatg
     2161 acaagatgct gaagctacgc aatcggctgc ttcagctgtt caagtattgc cgggttttcc
     2221 agtgtcgaaa catcctgggt tatttcttcg cctcttgcca gcgtgcgcag caggcgccgc
     2281 atgattttac cggaacgggt ctttggaagg ttatcgccaa accggatctc gtcgggacgg
     2341 gcgatggggc cgatttctcc tgagacccag ttgcgtaact caatggctgt tttttgagcg
     2401 tcggagcctt ggacgcgcga gcctttcaac accacgaatg ccacaatcgc ttcgcccttg
     2461 atttcatcag gcttgccgac taccgcagct tctgcgacga gaggactggc tacaagagcg
     2521 gattcgatct ccatggttcc cagccgatga ccggatacgt tgagcacatc gtcaatgcgt
     2581 cccatgatcc agaagtagcc atcgaggtcg cggttggccg agtccccggc gaggtaatac
     2641 ccgcctccga gctcttccgg gaagtaggtt ttcttgaagc gctccggatc accccacaac
     2701 gtgcgggcca aggatggaaa aggtcgtttg atgacaagga atccaccctt gccgtcgggc
     2761 acatcatgcc ctgcctcgtc gacaatcgct gccttgatgc caggcagggg aagcgtgcac
     2821 gagcccggct tgagcggtat ggcgccggga agcggggcaa tcatgtggct ccccgtttcg
     2881 gtctgccacc atgtatccac gaccgggcag cgggattgac caacggccgt gtaataccac
     2941 atccaggctt ccggattgat cggttcacct accgaaccca gcaggcgcag agaggagaga
     3001 tcgtattgac cgggaagatc ggaacccagc ttgatgagtg aacgaatggc agtgggcgca
     3061 gtataaaagg ttgtgacttt atggttttgg atcatcttcc agaaccggcc cgcatcagga
     3121 tgcgtcggta cgccttcaaa cacgacttgt gtcgccccga cagcgagggg gccatacgcg
     3181 acatagctgt ggccggttac ccagccaacg tcggccgtac accagaaaat atcggtaggc
     3241 ttgtaatcga acacccacaa catgcttacg atggttccca ggagatagcc cccagtcgag
     3301 tgctggactc ctttgggttt tcccgttgaa ccggaggtgt agagcgtgaa caacggatgc
     3361 tccgcattca cccactccgg ttcgcagccg tcgctctgcc cctggatcag ctcatgccac
     3421 cagacgtcgc gcggcgaatt taaccccaga ttcccctcac cccgcttgta aacgatgact
     3481 gacttcactg agtctgttcc gcccatcgca agggcttcag ctaccgcggc cttaagatgg
     3541 ttgatcttgc ccccgcggtg ttgctcatca gcggtaatga cggctaccgc accggcatcc
     3601 gtaatgcgtt catgcaggct ttttgccgaa aagcctccaa acaccaccga atgtgttgcg
     3661 ccgatgcgcg cgcaggcttg catcgcaacc acggcttcaa tgctcatggg catgtagata
     3721 atgactcggt ctcccttgcg g
//
LOCUS       DASSHJ010000017        17171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1406330,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000017 DASSHJ010000000
VERSION     DASSHJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406330"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..548
                     /locus_tag="VFI43_00975"
                     /pseudo
     CDS             306..548
                     /locus_tag="VFI43_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043765841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            774..1538
                     /locus_tag="VFI43_00980"
     CDS             774..1538
                     /locus_tag="VFI43_00980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7060732.1"
                     /translation="MLVDSHCHLDFPQLANNLDEALEKMRANDVSHALCISVNLKDFP
                     RVRALAESHSNLFASVGVHPDYEDLTEPKAAELSMLASHPRVIAIGETGLDYFRLEGD
                     LEWQRERFRQHIRAARQCGKPLVVHTRSAAADTLRIMAEEGADRVGGVMHCFTESWEV
                     AQQAMEMNFYISFSGIVTFKNATTLKDVARKINLDRMLVETDSPYLAPVPYRGQMNQP
                     AFVRHVAEAIAELRGISLDEVASATTANFFNLFKAA"
     gene            1541..2197
                     /locus_tag="VFI43_00985"
     CDS             1541..2197
                     /locus_tag="VFI43_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7060733.1"
                     /translation="MIPFRKLSFLAALMLVSNLYQQPLAVAGVYEDLRWAVEDNNTGD
                     IAKLIARGADPNTPDERGNTLLMVAVGHKNADLVDLLVNAGAKLNLRNRRGETAIMLA
                     SYRGFKDIVEKLYIRGAEINHPGWNPLIYAASGGHADIIRLLLEGGALVNSVSTNGTT
                     ALMMAARGNHEEAVKVLLEKGADPNLRNEWDMTALDLALKKKHYRVAELLKVRVSDAR
                     "
     gene            2228..2617
                     /locus_tag="VFI43_00990"
     CDS             2228..2617
                     /locus_tag="VFI43_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7060734.1"
                     /translation="MGAVGFNHFNLRAPGALLDELKTFYIEILGLAEGPRPSLGTPGH
                     WLYVGKQCVLHLSRTRPGEIRHTHIPTTFDHIAFTCIDLLEMKARLERNNIQFRTGQV
                     PSLGITQIFIQDPAGNGIELSFASEQA"
     gene            2710..3453
                     /locus_tag="VFI43_00995"
     CDS             2710..3453
                     /locus_tag="VFI43_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7060735.1"
                     /translation="MVKPLKDKVAIVTGSSRGIGAEIARTLGGAGAKVIVNYALNRPA
                     ADAVCLDITKTGGESLAVRADVSHAAEARILFDAAIGRFGRVHILVNNAGVLVSRKIA
                     DITDEEFDRVLAVNTKGVFHMLREAATRMDDGGRVVSLSSTVTRLMLPNYAAYAASKG
                     AVEQLTRVFAREVGERGITANIVSPGPVNTEMFTTGKTEETIRRMAQMSFEGRVGEPE
                     DIARAVLFLVSHDAGWITGQNISVSGGAA"
     gene            3986..5950
                     /locus_tag="VFI43_01000"
     CDS             3986..5950
                     /locus_tag="VFI43_01000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7060736.1"
                     /translation="MIGKTQEVELLKIKGRLPAPKGVALAIMEICRRDDATIAEIAKV
                     IQSDNALSARLIRLANSGANTGRPVNNIPDAIMRLGLSAVRLLALGFSLVDQYSSGPC
                     QAFDYTRFWSHSLLMAVACQQLGGYIKGGSPDELFACGLLARIGCLALATVYPAEYAE
                     LLTQQDGNTDADPTLLELERESLHVDHNELTATILASCNIPASLVEPVCYHEAPDASG
                     FAEGSRPYQLTHLFDHAKQLADLGLAPETERYSKTSELMLLGSKIGLDDEKMGAEVDR
                     IFLQWHTWGELLKVRTSTTPAFSRMAATPSHQPREERVPNQLRVILVDDDASNRLVME
                     GILGRILGRAIHSAGNGEEALAKARDLMPQIVVTNRLMPVMNGIEFCRSLRAAEWGQF
                     MYVIMLADGELEDEMVEAFEAGVNDYITKPVNIPALSARIRAALHYVKLLAAWERDRA
                     QLKRFAAELASSNSRLEKIELTDLLTGLPNLRAGMNALSKALLNAHRSSESLSVIMID
                     IDHFKSINERYGHALGDVVLKEVAMALQAKVRGNDVVCRMGGEKFFVICHNADPPSAF
                     HAAERLHATLRSLNIEIETVAIQITASIGVASREPQMLNRDAMLEAADKAMQAAKNSG
                     RDRIAVISQDRIHHPASGLQPEFHFAGNAV"
     gene            complement(5997..6245)
                     /locus_tag="VFI43_01005"
     CDS             complement(5997..6245)
                     /locus_tag="VFI43_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060737.1"
                     /translation="MKTIFDILMAAPPAQTTRCKIAMVEIAHGHWAAAALTMENAALE
                     TESGEWIRDCMQIRDFCILMDKVKYHGVEACARVQEVA"
     gene            6460..7167
                     /locus_tag="VFI43_01010"
     CDS             6460..7167
                     /locus_tag="VFI43_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7060738.1"
                     /translation="MPGGLINIDHATIALHLGAAVIAGGIIGLERTYHGHPAGFRTYA
                     LVCVASSLLMLVSLYEWEASSPGMSLGGIGAGPSRMAQGIMTGIGFLGAGVIFKEGLS
                     VRGLTTAASIWVTAAIGIMLGLGLYFPAGLATALTLGILSLFRMLEDGIPRHVYAQYH
                     ISFDRNNVMPEDMVRKLVIDHGFEIANMSYCINHAGLFEYRMDIRTTNPGSASTLARI
                     LLEKESVREFGIWTAGG"
     gene            7235..7660
                     /locus_tag="VFI43_01015"
     CDS             7235..7660
                     /locus_tag="VFI43_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7060739.1"
                     /translation="MQISPYLSFNGQCEAAFRFYEQRVGGKIVAMLTYGNSPAAEQCA
                     ADMHGKIMHARLILDGQTLMGSDSPHGRYEETRGMSVALGIDNPEKAEWIFSALAENG
                     TIQMPIQETFWAHRFGMLVDQFGIPWMINCERTDNKPGA"
     gene            complement(7675..8691)
                     /locus_tag="VFI43_01020"
     CDS             complement(7675..8691)
                     /locus_tag="VFI43_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET7060740.1"
                     /translation="MSDLPTGSGPAMLAMEIRQPGGPEVLMPVSRPIPQPGNGCLLIR
                     VRAAGVNRPDVLQRCGLYPPPPGASDIPGLEIAGEVIGLGGGTVRYKLGDKVCALLPA
                     GGYAEFCTVHETNALPVPQGLSMVQAAAVPETFFTVWANLFQRAGLQAGETVLIHGGA
                     SGIGTTAIMLAKAFGATVIVTVGSEEKRNACLRLGADFAINYGNQDFVEQTRELTGGK
                     GADVIVDIVAGDYVERNYKAAALNGRIAQIGVQKGPATNLNLMIMLHKRLTHTGSTLR
                     SRTIEEKAQIAGDLERHVWPLIEQRKVAPVIFKVFPLEEAGAAHALMESGEHVGKLVL
                     AIYG"
     gene            9306..9524
                     /locus_tag="VFI43_01025"
     CDS             9306..9524
                     /locus_tag="VFI43_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060741.1"
                     /translation="MDREEWYFQVGYVEILYAAMVVGIIVALYYFRRLKQVSEFAATL
                     IEEHGMLLGLLGLAIVLFILNRLLGDLW"
     gene            9565..10281
                     /locus_tag="VFI43_01030"
     CDS             9565..10281
                     /locus_tag="VFI43_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7060742.1"
                     /translation="MNILMVLTSHSQLDDTGEKTGLWLEEFTTPYYVFRDAGASVALA
                     SPEGGEPPIDPKSQRAENEGSSVRRFRRDPVAQAEFVNTRKLDGVAAAEFDAVFYPGG
                     HGPMWDLPENTASIGLIENFVGAEKPVAAVCHGPAALVNVRGKDGDYLVKGKRVTGFT
                     DAEEEAVGLASDVPFLLEDRLKERGAIFREEADWASHVEVDGKLVTGQNPASAGPAAE
                     ALLKLLGGGWGPGAPVQPMT"
     gene            complement(10266..11756)
                     /locus_tag="VFI43_01035"
     CDS             complement(10266..11756)
                     /locus_tag="VFI43_01035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7060743.1"
                     /translation="MEAHIQPGVKGTDPGGAYHGVILGAGVSGLCMGRALLAAGIKSF
                     VIIEKSAGIGGTWWDNRYPGAECDVPAHLYSYSFDPNPDWSHVYASRREIQQYLTRFA
                     DKSGLLPHIRLNAKLLEARFDEQKALWQLRLANGEQLLARHFICSAAPLSEPKYPEIP
                     GIKTFQGRLFHSACWDRNYPLSGTRVAVIGTAASAAQLVPRIAPLARKLYVCQRSPAW
                     IVPRANRSYTRLEKALFKVRPLSRMHRAVIYAIHEMDRLAFNPGGIVAKIGRGLAEFQ
                     LWRQVRNRALRAALRPDYPFGCKRVLLSNEYYPALTRSNVELVTSAIERIESDAILSG
                     DGQRREVDAIICATGFNIKHVLTSTRITGRKGISLNEAWANEPEAYKGVAVVGFPNLF
                     LLLGPNTGQGHTSAILYIEAQVNYVLQCLKELLQQDRSFIEVKPEAMAGYNEVLQKRL
                     ASSVWAAPCRSWYKTESGKIIGIYPGFSFEYMRELRKPRFEDYVIG"
     gene            complement(11845..12570)
                     /locus_tag="VFI43_01040"
     CDS             complement(11845..12570)
                     /locus_tag="VFI43_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7060744.1"
                     /translation="MHLSIVIPAFNEERLIEQCLNSISTSLKANYKSGFTSEVIVVDN
                     NSTDKTADLARQSGAQVVFEPVNQIGRARNAGAAAATGEWLLFVDADSKLNSGLLADI
                     LHLIEEGKSVGCGSTIEMRGLPWWANSSLRLWNAISVRYRWAAGALVVCRSDAFRDVG
                     GFNQELYAADEITLSEELAKWGQQRSLEFRILTEHALETSPRKVLLYSASEIFGQFFR
                     VILNPRRSLQDKSQLPIWYDGRR"
     gene            12716..12931
                     /locus_tag="VFI43_01045"
     CDS             12716..12931
                     /locus_tag="VFI43_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060745.1"
                     /translation="MSELGIIQWLAVSAVLFVLPFWRIAKRAGFKPGFAFLALVPGGI
                     LVLLWVIAFAKWPIFQQTNRIEEDKTA"
     gene            13731..14237
                     /locus_tag="VFI43_01050"
     CDS             13731..14237
                     /locus_tag="VFI43_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060746.1"
                     /translation="MKLIVLSIFMVLHAAAWAIDTSPIFEELDAETKFKDAQLHVKKL
                     MDTGQYQEASKYMPVYQRLACEAAEQKAGRAPADGSANCARRQNQGGINASVLQRQKE
                     LFSSAPASSGTAVPGVTVMTRPVTGSPTMLDSSAPSLLMFDPRNTPGTSSSESRPEPP
                     VDPWTGKR"
     gene            complement(14542..14811)
                     /locus_tag="VFI43_01055"
     CDS             complement(14542..14811)
                     /locus_tag="VFI43_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060747.1"
                     /translation="MEPEVVAGALEAVAAIAGVVVAAAVVGMGVGVGVVLGAVVVLAG
                     GLVVVVAVAALGTLEVAAVGVVAGAVVVAAGCFGCSMGTPVLRGT"
     gene            15139..15483
                     /locus_tag="VFI43_01060"
     CDS             15139..15483
                     /locus_tag="VFI43_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfJ-related protein"
                     /protein_id="HET7060748.1"
                     /translation="MKRSNLAIAFIIGFSLLSPLHSLAEKTAGNSSAATANIPANEGK
                     VLATLDAPGYTYMELANTEKRFWIAAPTTRVKVGDRVRFDQSLVMKNFNSKTLNRTFD
                     QIIFVNSATVIN"
     gene            complement(15789..>17171)
                     /locus_tag="VFI43_01065"
     CDS             complement(15789..>17171)
                     /locus_tag="VFI43_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7060749.1"
                     /translation="GSQDCDLTINLYNEKSTVWDLRKIIHDGLKERYPGTEFTFQPAD
                     LIAKILNFGSPSPIDVQVKGMDIYANYEYARKLQSKLRKIPGSSDVVIQQTMRTPTLL
                     VEGLRTFGLDVDRRESDIALNMLITTNGTQQIDQKYWLDLKTGISYQINIYDPQPQVA
                     SINDLNTIPVKREGEAALYEKYELLGNLTQESPVGTPGLVTHKGVMPLIEVYVSAEGR
                     GLGGVLEDVKKIVDEMKDEVPRGSTVKISGQAETMVSTYGELLIGLVAAVVLIYLMLV
                     INFQSWLIPFIIITALPGALAGIAWSLFLTQTNISVPALTGAIMAMGTATANSILVVS
                     YALERIAEHGDKLQAAVEAGKARIRPVLMTASAMIIGMLPMALGGSTNAPLGRAVIGG
                     LMFATVYTLFFVPCMYALIYHTRSVPKAKESVPQHGKRSRRRKAFMATRKPLLNSGIP
                     DMEKLGKKTR"
BASE COUNT         3950 a         4679 c         4567 g         3975 t
ORIGIN      
        1 tgtcttccag actatacctt gagaccttgg atgttattgg tgaccgtctc agtaacgtta
       61 ggcgccgctg ggagctttgg gccgagagtg gcgatgaagt tatgaagctc cttgccccaa
      121 caattcaaat tccacccttg cgtcggtcga gcagctttgc gccatcggtt cgcaactcgc
      181 ctctcggatc gacaaactat agagagtctg tcgtgtgacc gcgagctcgg cgaaaggtca
      241 gccaccttcg tctcaagctt tcccatcgcc gcctgagcaa ggcgcagctt cgccggcgtt
      301 atcttcgact ggaggtgatg cgccatgtcg gcatatccaa ccatgtgctt caggccgtcc
      361 cggcgcacag cgtgcgacgg atggcgcagg aaggccgccg actcagggca cagaacttcg
      421 agcagatgcg gcccgggcgc cggcatgcga cacttacgtc cttcctcttg gaaaccgaga
      481 tcgcgctcac cgatgcggcg atcgtcatgt ttgaaacgtt gatcggcaag agcttccgtc
      541 aggcggaatc gtaatcgtca gctcctcccg ccggctcgtc ggctcagtta aagattttcc
      601 ctgaatgttt gtgtcaaaag aagcgacatg atgttttttt cctgtgccgc gcccggttat
      661 tcctgctacg ctttcatttg ttgccgatag tgggataatt gacgctgcaa agcctgattg
      721 ctactcctga ttgccctact gttcgtaaga ctttctcttg ccatttttcg tgcatgctgg
      781 tcgattctca ttgccatctt gattttccac agctcgccaa taaccttgat gaggcgctcg
      841 aaaaaatgcg cgccaatgac gtcagccacg ccctttgcat aagcgttaat ctcaaagatt
      901 ttccgcgagt gcgcgcgctg gccgaaagcc attccaattt gttcgcatcg gtcggcgtcc
      961 atcccgatta cgaggacttg accgagccca aggctgccga actctccatg cttgccagtc
     1021 atccccgggt cattgcaatt ggcgaaaccg ggctggatta cttccgtctg gaaggcgacc
     1081 tggaatggca acgggaacgc ttccgccaac atattcgcgc tgcccggcaa tgcggcaaac
     1141 ctctggtcgt gcatacacgc tcggccgctg cggacacgct gcggatcatg gcggaggagg
     1201 gcgcggatag agtcggcggg gtgatgcatt gcttcactga aagctgggaa gtggcacagc
     1261 aggcaatgga aatgaacttt tacatttcat tctctggaat cgtcacattc aagaacgcaa
     1321 ccacgctcaa ggatgtcgca cgaaaaataa atctcgacag gatgctggtg gaaaccgact
     1381 ctccctatct ggcgcctgtg ccctaccgtg gccagatgaa ccagccggct ttcgtacggc
     1441 atgtcgcaga agccatagcg gaactccgcg gcataagcct ggacgaggtt gcatcagcca
     1501 ctaccgctaa ttttttcaac ttattcaagg cggcttgacc atgatcccat tccgaaagct
     1561 ttcttttctc gctgctttaa tgcttgtttc caatttatac cagcaaccgc tggccgtggc
     1621 gggtgtctac gaggatttac gctgggcggt cgaagacaac aacacggggg acattgccaa
     1681 actgatcgcc cgcggcgcgg atcccaatac tcccgatgaa cgcggcaaca cactgctgat
     1741 ggtggctgtc ggccacaaaa atgcggacct ggttgatctc ctggttaatg cgggcgccaa
     1801 gctcaacttg cgcaacagac ggggggagac ggccatcatg ctcgcgagct accggggctt
     1861 caaagacatt gtcgagaaac tttatataag gggtgccgaa atcaaccatc ccggctggaa
     1921 cccgctgatt tacgctgcat caggcgggca tgccgacatt attaggctat tgctggaggg
     1981 aggagcgctt gtcaactcgg tttccacaaa tggaacgacc gccctcatga tggctgcgcg
     2041 cggtaatcat gaggaagccg taaaggttct ccttgaaaaa ggggcggacc ccaatctcag
     2101 gaatgaatgg gacatgactg cgctggacct ggctttgaag aaaaaacatt atcgagtcgc
     2161 ggaactgctg aaggtgagag tgagcgatgc cagataaccc caagaagtaa ctttccgggt
     2221 agttctgatg ggcgcagtgg gtttcaatca ttttaattta cgtgctccag gcgctctgct
     2281 ggacgagctg aaaacgttct atatcgaaat tttaggtctg gcggaggggc cccgtccatc
     2341 cctgggaact cccggccatt ggctatatgt cgggaaacag tgcgttctcc acctgagtcg
     2401 gactcggcct ggcgaaatac ggcacaccca tatacccacc acttttgacc atatcgcttt
     2461 cacctgcatc gacctgctgg aaatgaaggc acgtctcgag cgcaataata tccagttcag
     2521 gacaggccag gtaccttcgt tgggaatcac ccagatattc atccaggatc ctgcgggaaa
     2581 tggaattgag ctgagttttg cgagcgagca agcgtgatag catgatgagt caacgcggaa
     2641 acgagatcgc gaagtactgc ttacctgact gccgatagga ctcatggtca cgacgctgat
     2701 ggatacctta tggtaaaacc acttaaagat aaggttgcga ttgttacagg ctcatcaagg
     2761 gggattggtg cggaaattgc ccgtacactg ggtggcgctg gtgcgaaagt catcgttaat
     2821 tacgctctta atcgacctgc agcagacgct gtttgcctgg atatcactaa aacgggaggt
     2881 gaatcgcttg ccgttcgggc cgacgtaagt cacgctgcag aggcgcgcat ccttttcgat
     2941 gccgcaattg ggcgctttgg ccgcgtacat attcttgtca ataatgccgg ggtgcttgtt
     3001 tcccggaaaa ttgcagacat aacggacgaa gagttcgacc gcgtcctggc cgtaaacacc
     3061 aaaggcgttt tccatatgct gcgcgaggcc gccacgcgga tggacgacgg tggccgggtg
     3121 gtcagccttt cgagcactgt aacgcgactg atgctgccca attacgcggc gtatgcggcg
     3181 agcaagggcg cagtcgagca gctcacgcgt gtttttgcaa gagaggtggg cgaaagggga
     3241 attaccgcga atatcgtatc gcccggtccg gtcaataccg agatgtttac gaccggcaaa
     3301 accgaggaaa cgatcagacg aatggcgcaa atgtcattcg agggacgggt tggtgagccg
     3361 gaggacattg cccgagcagt tctttttctc gtcagccacg acgcgggctg gattacaggg
     3421 caaaacatca gcgtcagtgg aggcgccgcg taagcaacct gtagtaccat cgcccgcttt
     3481 cctatcttga gtcccacaag ttttgtccgc cctggccgag atcggagcag tgtatgggta
     3541 ataccttcca agcagtattg ctacgagctt tatccggtca gcagctgagc attttcaaac
     3601 aatcgcccgg agcgcagcgc tccgtggatg acgcctgata gcgctcgcgg ctgttcatgc
     3661 agttccccgc gtcctcgccg aagcccgttt tgagaaccgg ccttttccac agacatccaa
     3721 acttgcgttg ggccgtcggt tgcaccgtca attgcatggt tcttttgttt cttatcagcg
     3781 cgcgattacc tgagcttggc agtcacaata agggtttcga atacgtgtcg tgctggaatc
     3841 atgaaataca ctgtgactcg gctgctgcta cccatgcggt tccaggtcgt catactatag
     3901 tgcttgaaag caataactca ccgtataccc gtatacctat acacccgtac acctgtcagg
     3961 atttaaaacc tccgatggat aaggcgtgat cggaaaaaca caagaggtcg agctcctcaa
     4021 aatcaaggga cgacttccag cccccaaagg cgtggcgctt gccataatgg aaatctgtcg
     4081 tcgcgacgat gcgacgatag cggaaatcgc caaggtaata caaagcgata acgccctgtc
     4141 tgctcgcctg atccgcttgg cgaattccgg cgccaacacg ggtcgtcccg tgaataacat
     4201 tcctgacgct atcatgcggc tgggactttc agcggttcgg ctactggcgt tgggtttctc
     4261 cctcgtcgat cagtattcaa gcgggccatg ccaggcattt gactacacgc gcttctggtc
     4321 tcattcattg ctgatggcag tggcctgcca gcagctgggc gggtatatca agggaggatc
     4381 gcccgacgag ctatttgcct gtggtttact ggcccgtatc ggctgcctgg ccctcgcgac
     4441 cgtttatcct gctgagtacg ctgaattatt gacgcaacag gatgggaata cggatgctga
     4501 tcccactttg cttgagctgg agcgtgaaag tctgcacgtc gatcataacg aactcactgc
     4561 caccatcctg gcgagttgca acattccagc atctctcgta gagccggtct gctaccatga
     4621 ggcccctgac gcctcgggct ttgccgaggg ctcacgacct taccagttga cccatctttt
     4681 cgatcatgcg aaacagttgg cggatctagg cctggcgccg gagacggaac gttacagcaa
     4741 gacatcggaa ctcatgctgc tggggagtaa aatcgggctg gacgacgaga aaatgggtgc
     4801 cgaggtcgac aggattttcc tgcagtggca cacgtggggc gaactgctta aggttcgcac
     4861 ctccacaact cccgcgttct caaggatggc ggctactcca tcgcatcaac ctcgagaaga
     4921 acgggtaccg aaccagctac gcgtaattct tgtcgacgat gatgcgtcga accggcttgt
     4981 aatggaaggc attctgggcc gcatactcgg ccgtgcgatc cattctgccg gcaatgggga
     5041 ggaggcgctg gcgaaggcac gggacctgat gccccagatc gtcgtgacca atcgactcat
     5101 gccggtcatg aacgggatcg agttttgccg cagcctgcgt gcggcggaat ggggccaatt
     5161 catgtacgtc atcatgctcg cagatggcga gttggaggac gagatggtcg aggccttcga
     5221 ggcgggcgtg aatgattata taaccaagcc ggtaaatatt cctgccctga gcgccaggat
     5281 tcgcgctgca ctgcactacg ttaaactgct tgcggcctgg gagcgggacc gagctcagct
     5341 caagcgattt gccgcagagc tggcgagcag caatagcagg ctggagaaaa tcgagttgac
     5401 ggatctgctc accggcctac ccaatctacg cgccgggatg aacgcgcttt cgaaagcgtt
     5461 gctcaacgcg catcgctcga gtgaatccct atcggtcatc atgatcgaca ttgatcattt
     5521 caagtcgatc aacgaacgct acggtcacgc cctgggcgat gtcgtattga aggaggtcgc
     5581 aatggcgctt caggccaagg tgaggggcaa tgacgttgtt tgccgcatgg gaggtgaaaa
     5641 atttttcgta atctgccaca acgcggatcc gccatccgcg tttcatgccg cggaacgact
     5701 gcatgctaca ctcagatccc tcaatatcga aatagagacc gtagctattc aaatcactgc
     5761 cagcatcggc gtagcatcca gggaacccca gatgttgaac cgggatgcaa tgttggaagc
     5821 agccgataag gcgatgcaag cagccaaaaa ttctggccgg gaccggatcg ccgtcatcag
     5881 ccaggaccgg atacaccacc ccgcgagcgg gcttcagccg gaatttcatt ttgccggaaa
     5941 tgcggtttag ccggcgttgc cgggattacc gtacattaat cgaggccaag gggcggctac
     6001 gccacttctt gcacccgcgc acaagcctcg accccgtgat atttcacctt atccatcagg
     6061 atgcagaaat cccggatctg catgcagtct cgtatccatt ctccggactc cgtttccaac
     6121 gcggcgtttt ccatggtcag cgcggcggca gcccaatgtc cgtgcgcaat ttctaccatt
     6181 gcgatcttgc accgtgtggt ttgcgcaggt ggtgccgcca taaggatatc gaatatcgtt
     6241 ttcacggcga cctctattcg taaaacctaa accccctcaa acaatttgac catgcatagg
     6301 ggggccaagt gccgcacggg aaattatttt atgcaataac ccggatccgg agagacatgt
     6361 cccgggcagt ggacatatta tttctaattg agtacagtga gttatgccaa atatttaaat
     6421 atttcatgaa gaacggacga atgtgtctcg caacagcatg tgccgggagg tctcattaac
     6481 atcgaccatg ccaccattgc ccttcatctt ggcgcagcag tgattgccgg gggtatcatt
     6541 ggtctcgaaa ggacctatca cgggcatcct gccggatttc gaacgtacgc acttgtttgc
     6601 gtggcctcca gcttattgat gctggtttcc ctttatgaat gggaggcgtc ctctcctggc
     6661 atgtcactcg gcgggatcgg tgctggtccc agcagaatgg cgcaaggcat catgacagga
     6721 ataggatttc ttggcgcggg ggtaatattc aaggagggtt taagcgtacg tgggctcaca
     6781 accgcggcat ccatctgggt aactgccgct attggcatca tgctgggcct cggcctttat
     6841 tttccggcgg gacttgcaac cgcgctaacc ctgggaatcc tttcgctgtt ccgcatgctt
     6901 gaagatggga ttccacgtca cgtctatgcg caataccata tcagctttga tagaaataac
     6961 gtaatgcccg aggatatggt tcgaaaactt gtgatcgacc acggatttga aatagccaat
     7021 atgagctatt gcatcaacca tgcggggctg tttgaatatc gtatggatat ccggaccacg
     7081 aaccccggca gcgcatccac gttggcacga atattgcttg agaaagaatc tgttcgcgag
     7141 ttcggaatct ggaccgctgg gggataggag cgcaatcctc tccctcccag cgccatgagg
     7201 ctgacgattt tttcgataaa aaaggagttc aacaatgcag ataagtcctt atttaagttt
     7261 taacggtcag tgtgaggcgg cgttcaggtt ctatgagcaa cgtgtgggag gaaaaatcgt
     7321 cgccatgctg acatacggaa actcaccggc tgcagaacag tgcgctgccg atatgcacgg
     7381 aaagatcatg catgcccggc tgatcctgga cggccaaaca ttgatgggtt cagacagtcc
     7441 gcacgggcgc tatgaagaaa ccagaggcat gtcggttgcg ctggggatag acaatcctga
     7501 aaaggctgag tggatattca gtgcgttggc ggaaaatgga acgatacaga tgccgattca
     7561 ggagaccttc tgggcccatc gtttcggaat gctggtagat cagttcggaa tcccgtggat
     7621 gatcaattgc gagcgcacgg ataacaaacc cggcgcatag aactgcaagg cgccttaccc
     7681 gtaaattgca agaacgagtt tgcccacatg ctcgcctgat tccatcagcg catgcgcggc
     7741 accggcctct tccagcggaa aaaccttgaa tatcaccggg gccacttttc tttgctctat
     7801 gagaggccat acgtgccgtt ccagatcccc tgcaatctgt gccttttcct cgatagtgcg
     7861 cgatcgcagc gtggaaccgg tgtgcgtcag ccgtttatga agcatgatca tcaggttcag
     7921 gtttgtagcc ggccccttct gtactccgat ctgcgcgatg cgcccgttca gcgccgccgc
     7981 cttgtaattc cgttcgacat aatctcccgc gacgatatcc acaatgacgt cggccccctt
     8041 gccgcctgtg agctcccggg tttgctcgac aaaatcctga ttaccgtagt tgatggcgaa
     8101 atccgcaccc aaccgaaggc aggcattccg tttttcttct gacccgacgg ttacgataac
     8161 cgtcgcccca aatgccttgg ccaacatgat tgccgtggtt cctatgccag aagcgccgcc
     8221 atggatcaat actgtctccc cggcctggag tcccgctctc tgaaagagat ttgcccagac
     8281 ggtgaagaat gtttcgggca cggcggcggc ctgcaccata ctcaaccctt ggggcaccgg
     8341 gagcgcattg gtttcgtgta cggtacagaa ttcggcgtac ccccctgcgg gaagcagggc
     8401 gcaaaccttg tcacccagct tatagcgcac cgtaccacca ccaagcccga tcacctctcc
     8461 ggcgatttca agacctggga tgtcggacgc accaggggga ggagggtaga gcccgcaacg
     8521 ctgcaagacg tctggacggt tcacgcccgc cgcacgaact ctgatcaaca ggcaaccgtt
     8581 gcccggctgc ggaatcggac gcgagacggg catgagcacc tccggcccgc ccggctgcct
     8641 gatttccata gcaagcatcg caggaccact gcctgtcggc aaatcactca ctgtacgatc
     8701 atggaatcaa taactcatgc tgttgagagg cgagtccggg ataagcggct gtgtccgaac
     8761 tgtccggagc acccctcctg ttacctgatt gactcgtgtg gcatacatta tctttggccc
     8821 aattgccata atctgtctgc tgaaccacta acttttcatt cacagaattt cgaaaagaag
     8881 ttatgttcca ttgcaaacgg gagtgccaac acgatgaaaa aggccattcc tgaaatcttt
     8941 ctccggaatg acttattggg gaacaagggc aggccgggga aagaaacgcg taagttcctg
     9001 gcgaaaatcc gctacggagc taatatttaa ggaacctctc agtaagtccc gcatgggtgc
     9061 gctgctagca agatcgggat gatcacaagg cgtgacgaag gtcgccacgc gcgtaggatg
     9121 ccaggaagcg caacgccgag ggctattgat gtcgcaccaa gcagccgctg taccgttctc
     9181 tattccccat tcccgttgcc ggaaagacct taaccatttt tgaagcgctt ctttgactta
     9241 tcgataattc gtcggccttt ccagcgggtt tgataaaaac cgctccggga attggagaaa
     9301 aatgcatgga tagggaagaa tggtactttc aggtaggcta tgtagaaatt ttgtatgccg
     9361 caatggtggt cgggatcatc gtcgctttat attacttcag gcgactgaag caggtttccg
     9421 agtttgccgc caccctgatc gaagagcatg gcatgctgct cggtttgctc ggactggcta
     9481 tcgttctgtt tatcctgaac aggttgctgg gagacttgtg gtgattttcg taaaacaaca
     9541 aatgaatgtt aaaggagaaa ccctatgaat attctcatgg tcttgacatc tcacagccag
     9601 ctcgacgata cgggcgagaa aacaggctta tggctggaag aatttaccac gccttactac
     9661 gtattcaggg atgccggcgc cagcgtcgcg cttgcgtccc ctgaaggagg ggaacctccc
     9721 atcgatccga aaagccagcg cgcggagaat gaggggtcgt cggtacgacg cttccgcaga
     9781 gatcccgtcg cacaggccga gttcgtgaac accaggaagc tggatggggt ggctgccgcg
     9841 gaattcgacg ccgtcttcta cccgggagga cacgggccga tgtgggattt gcctgagaat
     9901 accgcttcta tcggactgat cgagaatttc gtgggcgctg aaaaacccgt cgccgcagtt
     9961 tgtcatggac ctgctgcact ggtgaatgtt cgcgggaaag atggggatta tctggtcaag
    10021 ggaaagaggg tgacagggtt taccgacgcg gaggaagagg cggtcggtct ggcctctgac
    10081 gtgccttttc tcctcgagga tcggctcaag gagcggggcg ctatctttcg ggaggaggct
    10141 gattgggctt ctcatgtcga agtggatggg aagcttgtaa ccggccagaa tccggcttcc
    10201 gcgggccccg cagcagaagc cttgctaaaa cttcttggtg gaggatgggg accgggagct
    10261 ccagttcaac caatgacata gtcttcgaat cggggtttcc gaagctcccg catatattcg
    10321 aaagaaaaac cggggtagat cccgataatc ttccccgatt cggtcttgta ccagctcctg
    10381 caaggtgccg cccagaccga tgaagcgagc ctcttctgta aaacctcgtt ataaccggcc
    10441 atcgcctctg gtttcacctc gataaaactc ctgtcctgtt gcaacagttc cttgaggcat
    10501 tgcaggacat agttcacctg cgcttcgata tacaggattg ccgaggtatg tccttgtccc
    10561 gtgttggggc cgagaagcag gaacaggttg ggaaagccga ctaccgctac ccccttatag
    10621 gcctccggct cattcgccca ggcttcattc agggatatcc ctttccggcc cgtaatcctt
    10681 gttgaagtca atacgtgctt gatattgaat ccggtggcac aaataattgc gtccacttcc
    10741 cgcctttgac cgtcgccgga taagatcgca tccgattcga tccgctcgat cgccgaagtg
    10801 acgagttcca cgtttgagcg cgtgagggca gggtagtact cgttggaaag gagaacccgt
    10861 ttgcatccga aggggtagtc agggcgaagg gcggcacgca gtgccctgtt gcggacttgc
    10921 cgccagagct gaaactccgc cagcccacgt ccgattttgg caacgatacc accgggatta
    10981 aaagcgagcc gatccatttc gtgaattgca taaatgacgg ctcgatgcat acgggacaga
    11041 ggcctcacct tgaacagagc tttttccagt cgcgtatagg atcggtttgc tctcggcacg
    11101 atccaggcgg gagagcgctg gcagacgtac aacttccttg ccagcggggc aatgcgtgga
    11161 accaactggg cggcgctcgc agcggttccg ataactgcaa ctcgcgttcc actcaacggg
    11221 tagttgcgat cccaacacgc agagtgaaac agcctgccct gaaaagtctt gataccggga
    11281 atctccggat atttcggctc gctcagcggc gccgcgctgc aaatgaagtg acgcgccaat
    11341 agttgctcgc cgtttgcgag ccgcagctgc cacagagcct tttgctcatc aaaccgtgct
    11401 tcaagcagct tagcatttaa ccggatatgc gggagcagac cggatttatc tgcaaaccgc
    11461 gtgagatatt gctgaatttc ccgccgcgac gcataaacgt gcgaccagtc aggattcggg
    11521 tcaaatgaat atgagtaaag atgtgcggga acatcacact ccgctcccgg atacctgtta
    11581 tcccaccacg tgcccccgat gccagcggac ttttcgataa taacaaagga cttgatgccc
    11641 gctgcaagca gcgctcttcc catgcaaagt ccagacacgc ccgcgccgag aattacccca
    11701 tgatacgcgc ctccagggtc agtacctttc actcccggct ggatatgtgc ttccatggcg
    11761 atactcgctc gctcagaggg attcaagtat ttccacttcg gcacattagc gtcaccacag
    11821 cctcaaaaag caaggcaaga acttctagcg ccgcccgtca taccagattg gtaactggct
    11881 cttgtcctgc aatgaccggc gggggttgag aataacccga aagaactgac cgaaaatttc
    11941 cgatgcagag tagagcaaga cctttcgcgg ggacgtttcc agggcatgct ctgtcaaaat
    12001 cctgaattca agacttcgtt gctggcccca tttggctaac tcctcgctca aggtgatctc
    12061 gtcggcagcg tagagttcct ggttgaagcc gcccacgtcg cgaaacgcat cgcttcggca
    12121 cactaccaat gctccagccg cccagcgata tctaaccgat atcgcattcc agagccgtaa
    12181 gctcgaattt gcccaccacg gaaggcctct catttctatc gtgctgccac agcctacact
    12241 cttgccttcc tcgatcaggt gcaatatgtc ggcaagcaat ccggaattga gcttgctatc
    12301 ggcgtctacg aacagcaacc actctcccgt ggcagcagca gcgcccgcat ttcgtgcgcg
    12361 accgatctga ttcaccggct cgaaaaccac ctgcgcgcca gattgcctcg cgagatccgc
    12421 cgttttatcc gtggaattgt tatccacgac gatcacctct gaggtaaagc cggacttgta
    12481 attcgctttc aatgatgtag aaatagagtt cagacactgc tcgatcaggc gttcctcgtt
    12541 aaacgctgga atgacgatgg agagatgcat aagggaaatt gacgttgatg tattgaagct
    12601 gaattatcac cgaatttagc cgttgccgtc cttttttctc tcggttccag gcctcgatga
    12661 ttgaaaaaac gagcacgagc cgagagaatg gattcaggat tgactggagt cgattatgag
    12721 cgagcttggg atcatacagt ggttggcggt atcggccgtt ctctttgtcc ttcctttctg
    12781 gaggatcgcc aaacgcgcgg gcttcaagcc cggcttcgct tttcttgcac ttgttcccgg
    12841 tggcatcctg gtgctcctct gggtgatcgc tttcgcgaag tggccaatat tccagcagac
    12901 aaacaggata gaagaggaca agacggcctg aacggcaaca ccgagtcgac tcggcaccgc
    12961 ctgcattcgt cacactggaa caatcctcag cctgaagcct gtgtcgctcg gggcggcgaa
    13021 attttacatg cagaaccagg tagaggcggg acactgttgc ccgataacca tgacatcagc
    13081 agccatcccg gcgctttgcc agcagccgga tatcgcgacc atacgggagg agaagatcct
    13141 tgatcgccac taccctcgga acgtgccggc gtgcgacacc gtcattggtc attgctgatg
    13201 ccgatgcgga cggtgaatcg tcgctcgcaa gcgacaaggt atagggggtt ttgcatatgc
    13261 tgttttttat tagccagtta ggatgtagcc ggtacacgac actcccttta ccttttgtcg
    13321 tttcttatac aatttaacat aatacacatt atacgcattt tgaacggctt ccaggaagga
    13381 acgcttcggg gccatgggat aaaaatcacc cttatgtgcg gttgggtaca gaatacatcg
    13441 gaccgtttcg ccagaataaa gtcagacgtc tgctttttct ccctcttgtg gtagacgtca
    13501 ttggcccggc aaaggccaac tactccttgc cgggcctttt tatgtctcag ggctccccgc
    13561 aggaacaccg gcgtcaaaag attcagcgct tctgatcgat tgcgaaaatc gctatccggg
    13621 agatttggaa caacttccgg aacaagtgct gtaatgcttc atcagacaac tatttctttt
    13681 ctgtatattc gttctataat tctcatgact ccacttgcaa gggggaatac atgaaactta
    13741 tagttctgtc tattttcatg gtattgcatg cagccgcatg ggcgattgat acgtcgccga
    13801 tcttcgagga actggatgca gagacgaagt tcaaggatgc ccagcttcac gtgaaaaaac
    13861 tgatggatac tggtcaatat caggaagcgt ccaaatacat gcctgtttac caaagattgg
    13921 catgcgaagc tgctgaacaa aaggccggca gagctcctgc cgacggctcg gcaaattgcg
    13981 cccgtaggca aaatcagggc gggatcaacg ccagcgtgct ccagcggcag aaggaactat
    14041 tcagctctgc gcctgcatct tccggtactg ctgtccccgg cgtcaccgta atgacacggc
    14101 ccgtcacagg gtccccgacg atgctggaca gttcggcgcc ttcgctgctc atgtttgatc
    14161 ctcgaaacac ccctggaacc tcttcttctg agtcgcggcc cgagccgcca gtcgatccct
    14221 ggaccgggaa gcggtgagat cccatccgtt ttaccatttc ctgccattaa gcaactgctt
    14281 tcgctttcgg caaacggctt gaccccgttt gtcgaagatt cttacacagg caaatcctcg
    14341 aatattacct catccacggc ctagggcgtg taacggcgct tttcgtgcgt ccttcagata
    14401 tggcctgatt cttgcttcat cattttcttt agaagaatcc ggttattcgt tatacccatc
    14461 gtgaggaata ctgattatgc aaagaatgat actggtagta tttgcctcat tcctggcaac
    14521 tgctgtagcg caggccgtgg atcaggtgcc gcgcaagaca ggtgtaccca tgctacagcc
    14581 gaaacagcca gccgcaacca ctactgcccc tgcaacgact cccactgcag cgacttcaag
    14641 tgttcccaag gcagcgactg caacgacaac tacaagtcca cccgctaata cgaccactgc
    14701 ccctaaaacc acacctacac cgacacccat acccacgacc gcagctgcta ccacgactcc
    14761 cgcgatggca gccactgctt ccaatgctcc tgcaacaact tctggttcca agccctggac
    14821 acctcccccg gggcgccttc cggattccgt ggtaacaaac ctcactttcg ccagcgacaa
    14881 aaatcagcaa ttggcttcag gccctcccgg atttcaggtt aatcaggtac ctgaaccggg
    14941 gacgatgttg cttattggta ttggtgtagt gggtttggca atctcccggc gccgcaaggt
    15001 ataaagttat tgcgcacgct ttcatcctgg gtaagggatg gtcggcgcat ctgattggga
    15061 attgcagtat ggaaaccata cggattgcgg tgtattatgc gtgcttccga aactcacacc
    15121 cgatcttggg aggctgtttt gaagagatcc aatctggcga ttgcttttat tatcggattt
    15181 tccctcctta gcccgttgca ctcattggcg gaaaagactg cgggcaactc ttcagccgcg
    15241 accgccaata taccagccaa cgaaggtaaa gtgctcgcaa cccttgatgc cccgggctat
    15301 acgtatatgg agcttgccaa caccgaaaag cgtttctgga tagcggcgcc cacaacgcgg
    15361 gtaaaggtgg gcgacagggt ccgattcgat caaagcctgg tgatgaagaa tttcaatagt
    15421 aaaacattga accggacctt tgaccaaatc atatttgtta actcggcaac tgttatcaat
    15481 tagtaaaagt aaagaagcct ggatcaccta tcgacatagc atttatccgc ctatcaagga
    15541 gcccccagca cttcagatac cctcttttaa gaaacctcac tcctccccgc tgacttgcac
    15601 cttttcgtaa tttcccttca catcgcttca agtaagtcga ttccgattcc actcttattc
    15661 ttacctcact ccgcgcgctt tggctgagcg ctggaaatac tgctgtcctt tcactgtgca
    15721 attctaatgg tgcggttagc catccggcgc tccattcata tagacaacac caagaacgat
    15781 cgcaataatt accgtgtttt tttacctaat ttttccatat caggaattcc cgagttgagg
    15841 agcggtttcc ttgtcgccat aaacgctttc cgtcgccggg aacgctttcc atgttgagga
    15901 acgctttcct tcgctttagg aacgctccgt gtatgataga taagggcata catacagggg
    15961 acaaaaaaca gggtatatac cgttgcaaac atcaaaccgc caataactgc acggcccaag
    16021 ggcgcattgg tggatcctcc caacgccatc ggaagcatgc cgatgatcat ggctgatgca
    16081 gtcatgagaa caggacgaat ccgggctttg ccggcctcaa cggcggcttg cagcttgtcg
    16141 ccatgctccg cgattcgctc cagcgcatag gacacgacaa gtatggaatt tgccgtggcc
    16201 gttcccatgg ccatgatggc gcctgtcagg gccgggacgg aaatattcgt ctgggtcagg
    16261 aacagggacc aggcaatgcc tgccaaagcg ccgggcaggg ctgtaatgat gatgaaggga
    16321 atcagccatg attgaaaatt gatgacaagc atgagataga tcagcaccac agccgctacg
    16381 aggccgataa gcagttctcc ataggtactg accatagtct cggcctggcc gctgatttta
    16441 acagtggagc cgcggggaac ctcatctttc atctcgtcga caattttctt gacgtcctcc
    16501 agcaccccgc caagcccccg tccttcggca gatacataaa cttcaatcaa cggcatgaca
    16561 cccttgtggg tgaccagacc gggcgtgcct accggcgatt cctgcgtcag gttgcccagg
    16621 agttcatact tctcatatag ggcggcttcg ccctcccgtt tgacaggaat cgtgttcaag
    16681 tcattgatgc ttgcaacctg aggttgagga tcatatatgt tgatctggta tgagatgccg
    16741 gtcttcaggt ccagccagta tttctggtca atctgctggg ttccgttagt cgttatcagc
    16801 atattcaagg caatatccga ttctctcctg tcaacatcga gtccaaatgt ccttaggcct
    16861 tcaacaagca gggttggcgt gcgcatggtt tgctggatca ccacatccga ggaaccgggg
    16921 attttacgga gcttgctttg caatttgcgc gcgtattcgt agtttgcgta tatatccatc
    16981 cccttaacct gaacatcaat cggagaaggg gagccaaaat tcaagatttt cgctatcaga
    17041 tcggctggct ggaatgtgaa ttcagttccc gggtagcgct ccttcagacc atcatggata
    17101 attttccgga gatcccatac tgttgatttt tcgttataca ggttgattgt cagatcgcag
    17161 tcctgtgatc c
//
LOCUS       DASSHJ010000018         5321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_543138, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000018 DASSHJ010000000
VERSION     DASSHJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543138"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFI43_01070"
     CDS             <1..377
                     /locus_tag="VFI43_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060750.1"
                     /translation="YGCLDFQIETPQLKIEQRSHSYRRAHPEYPDSAIGGEAQFAISM
                     VLGKECAGLALIIYQHCPVQSELIVEGRAMRAKDRVDEVSTVGRFLGLARGARDRGYQ
                     HSNTAELKFRLVDVGLPRARLK"
     gene            497..3106
                     /gene="lon"
                     /locus_tag="VFI43_01075"
     CDS             497..3106
                     /gene="lon"
                     /locus_tag="VFI43_01075"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7060751.1"
                     /translation="MGELIVARHDLPEDVIALVPMRNVVLFPHVLTAITVGRAKSIAA
                     LEHAIKAHVPLGIVLQRDPAVDDPGLDSLFSMGTLVNVVRHLASSDGLRHAVCQGIER
                     FVIEELVEGYPFLAVKIRRIGEPAEVSTEAEALAMQLRERAVEILSLLPGVPAELAQA
                     LQAARAPSHLADIVASLLDTEVNEKQILLETVSTEERLRKVLQILSRRIEVLRLSHEI
                     GERTKEHLEDRERKFLLREQLKTIQKELGETEEDDQEIAELSEAIDKAGMPEETEAQA
                     RKELQRLQRMPPASSEYSMLHTYLEWMTELPWKLPPDAPINLDAARQILEADHFGLER
                     VKQRIIEFLAVQKLKPQGRAPILCFVGPPGVGKTSLGQSIARALERPFVRVSLGGVHD
                     EAEMRGHRRTYIGAMPGNIIQSLRKAGARNCVMMLDEVDKMSASMHGDPSAALLEVLD
                     PEQNSTFRDNYLGVPFDLSRVVFITTANVIDNVPPPVRDRMEIIDLPGYTREEKLQIA
                     LRYLVNRQREANGLAEGQCEITIAALESIIANYTREAGVRQLEREIGRVMRSAAVRVA
                     EDANSQVRVDAADLDAILGAPKFEHEIGLRASLPGVATGLAWTPVGGDILFIEATRVN
                     GRGQLILTGQLGGVMKESAQAALTLVKARADDLGIPISIFDHIDVHVHVPAGAIPKDG
                     PSAGVAMFIALASLFTDRPVNHEVAMTGEISLRGMVLPVGGIKEKVLAAQRAGLRTVL
                     MPARNEKDLRDVPEGAREALQFIFLETVDDAIQASLGQRGVRRGGRSSGWFEVHWQKP
                     SRRETRCGSTEKTVFIPTLGEVGEKVILFVEGVGYIWKSLYPCGVSASPISWARRPIR
                     IHRKLASWQPKLR"
     gene            complement(4031..4759)
                     /locus_tag="VFI43_01080"
     CDS             complement(4031..4759)
                     /locus_tag="VFI43_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET7060752.1"
                     /translation="MMDRRKLLGLLGATGLDMVSGYSLGKADEKEAGPKMFSCVPTPQ
                     QTEGPYFVDERLRRADIRSDPSDGAIKEGVLLALNMHVFSVGKTACRPIANAIIDIWQ
                     CDARGIYSDVVDSHFNTMGKKFLRGYQVTDADGGAQFLTIYPGWYPGRTVHIHFKIRT
                     DPKTARAYEFTSQLYFDDALTDRVHEHPAYAQRQQKPGKLSRTKNEEDGIFRKGGMQL
                     ILKPAKTNHGYAGSFDVGLEMGDA"
     gene            complement(4954..>5321)
                     /locus_tag="VFI43_01085"
     CDS             complement(4954..>5321)
                     /locus_tag="VFI43_01085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7060753.1"
                     /translation="NKIVGTTRFYNVDESNRRPTLGYTWYAKSACKTSVNTESKLLLL
                     RYAFEQKAAIAVEFRTHYFNQVSRAAIERLGAKQDGVLRSHQIMRDGSIRDTVVYSIL
                     EHEWPAVKNNLMNKLALDV"
BASE COUNT         1169 a         1438 c         1489 g         1225 t
ORIGIN      
        1 tctacggttg ccttgatttt caaatcgaaa ctcctcagtt aaaaattgaa cagcggagtc
       61 actcttatag acgggctcac cctgaatacc cggactcagc aataggcgga gaagcgcaat
      121 tcgcgatcag catggtactc ggaaaagagt gtgctggact ggctctgatt atctatcaac
      181 attgtccagt tcagtcagaa ctgattgtag aaggtcgcgc catgcgcgca aaggataggg
      241 tcgatgaggt ctccactgtg ggacgctttc ttggtcttgc ccgtggtgca agggataggg
      301 gttatcagca ctcgaatacc gcagagctga agttccggct ggttgacgtg gggttgcccc
      361 gtgctagact gaaatgatca ttatctgttt cctcggcgaa ccgacacgtg acgcccggcg
      421 agccgcggga gtttccggtt ctcttgttta acatgagagt gatcaaaaaa ccgtattcat
      481 aaccgggagg gccgccgtgg gagagcttat cgtagccagg cacgacttac ccgaggacgt
      541 catcgcgctg gttcccatgc gcaacgtcgt attattcccg catgtgctga cggccatcac
      601 cgtggggcgg gcgaagtcca tcgccgcgct ggagcacgcc ataaaagctc atgtcccgct
      661 cggaattgtt ttgcagcgcg atccggccgt cgatgacccg ggcctcgatt cactgttcag
      721 catgggcacg ctcgtcaacg tcgttcggca tcttgcgtcc tcggatggat tgagacacgc
      781 ggtctgccag ggtatcgaaa ggtttgtcat cgaagagctg gttgaaggat atcccttcct
      841 ggccgtcaag atccgacgca ttggtgaacc ggctgaagta tccactgaag ccgaggccct
      901 cgccatgcaa ctgcgggagc gcgcggttga gatcctgtca ctgttgcccg gcgtgcccgc
      961 ggagctcgcg caggccttgc aggcggcacg ggcgccatcc catctggccg acatcgtcgc
     1021 cagtctgctt gacacagaag tgaacgagaa gcagatattg cttgagaccg tgagtacgga
     1081 agagcgcttg cggaaagtac tccagattct gtcgcgccgc atagaggtac tcaggctctc
     1141 ccatgaaatc ggcgaacgca ccaaggagca cctcgaggac cgcgaacgca agttcctatt
     1201 gcgcgagcaa ttgaaaacca tacagaagga gcttggggaa accgaagagg acgatcagga
     1261 aatcgcagag ttgagcgaag cgatcgacaa ggcgggcatg cccgaagaga ctgaggcgca
     1321 ggcacgcaag gaactgcagc ggctccagcg gatgcctccc gcgtccagcg agtattccat
     1381 gctgcatacc tatctggagt ggatgaccga gctgccctgg aagctgccgc ctgatgcccc
     1441 cattaacctg gacgcggctc ggcagattct tgaagcggat cactttgggc tggagcgcgt
     1501 caagcagcgc attatcgaat tcttggcagt acaaaaattg aagccgcagg gacgcgcccc
     1561 tatcctgtgc ttcgtaggtc cccccggagt gggcaagaca tccctcggcc agagtatcgc
     1621 ccgggcattg gagcggccgt tcgtccgcgt gtcgctgggt ggtgttcatg atgaggctga
     1681 gatgcgcggt cacaggcgca cctacatcgg cgcaatgccg ggaaacatca tccagagcct
     1741 tcgcaaggcg ggagcccgca attgcgtcat gatgctcgac gaagtcgaca aaatgtccgc
     1801 gagcatgcat ggcgacccct cggcggcgct gctcgaagtg ctggaccccg agcagaattc
     1861 gacttttcgc gacaattacc tcggcgttcc cttcgatctg agccgcgtgg tgttcattac
     1921 gaccgcgaac gtgatcgata acgtaccgcc ccccgtgcgc gatcgcatgg aaatcatcga
     1981 tctgcccggc tatacccgtg aggaaaagct ccagatcgcg ctgcgctacc tggtgaatcg
     2041 gcaacgcgag gccaacggat tggcagaggg gcaatgtgaa atcacaatcg cggccttgga
     2101 aagcattatt gccaactaca cccgggaggc gggcgtgcgc cagcttgagc gggagatcgg
     2161 gcgcgtcatg cgttccgcag cggtgcgtgt tgcagaagat gcaaacagcc aggtgcgagt
     2221 cgacgcggca gatctggatg cgatattagg ggcacccaaa ttcgagcatg aaatcggctt
     2281 gcgcgcgagt ctgccggggg ttgcgacagg gcttgcgtgg acgcccgtgg ggggcgatat
     2341 tctttttatc gaggctacgc gggtcaacgg tcgaggacag ctcatcctga ctggccagtt
     2401 ggggggcgtg atgaaggaaa gcgcccaagc cgcgcttacc ctggtgaaag cgcgcgcaga
     2461 tgatcttggc ataccgatat caattttcga ccatattgac gtgcatgtcc atgtgcccgc
     2521 gggcgccatt cccaaggatg gcccaagcgc aggcgtcgcc atgttcatag cgcttgcgtc
     2581 gctttttacc gaccgccccg tgaaccatga ggtcgcaatg acgggcgaaa tcagcctcag
     2641 gggaatggtg ttaccggttg gcggcatcaa ggaaaaagtg ctcgcggccc agcgcgcggg
     2701 cctgcgtaca gtactcatgc cggcgcgtaa cgaaaaagac ttgcgcgatg tcccggaagg
     2761 cgcgcgcgag gcgttgcaat tcatcttcct ggaaaccgtc gacgatgcca tccaggcgtc
     2821 tctcggtcaa cgcggcgttc gtcggggggg ccggagttca ggctggtttg aggttcattg
     2881 gcagaaaccg tcacggaggg aaacgcgttg tggcagtacc gaaaaaaccg ttttcattcc
     2941 cacgcttggg gaggttggcg agaaggtgat tctttttgtg gaaggggttg gctatatatg
     3001 gaagagcctt tatccatgcg gggtttcagc ctcgcctatt tcttgggctc gtcgccctat
     3061 aagaatacac agaaaattag catcctggca gccaaaacta cgctaaccac gagtaggccg
     3121 tagaacaagc gcgcacgcaa agggtgttca acgtctaatt tcaagatgaa gccaataaac
     3181 aggctaccca gcagcgagat aatcaaaata ctccaagcta ttggatgggt ggcgtaccgg
     3241 cactgttttt caggatcagg tacccctgtc atagtgacat tcgatgcaag aaccgggtca
     3301 gcggccagag ccaagcaaat aatcgccaaa acaaccgggg caaggcgcac agaatcgcgt
     3361 ttgaatatct ccagtatcgc ttcgccatgg gctgtcagtt ggtggcaagt ccaattcatc
     3421 atttccctca tcaagtgagc gcttccctat tcaaccgata aataaagaag aatcttcagc
     3481 ttttcgtgat ctcttttcgg cttaggtata ccctactcgg acagtaggtg tcggccgagg
     3541 cttactgttt tggcaatccc tttttctctt gtactttgtt tttaaatccc gtcatttctt
     3601 caaatagacg atctacctca cggccatccg gccccgcgtc aagttgtact tcgccacacg
     3661 ccaggcaatg ccaggcaata cttcccccgt gcctgttttt cctagtcgtt atacccctat
     3721 agctgtagga tatatcgtac atcacctgcc agttcatccg taccgcctga cccatatatc
     3781 atgatttttc cgtcccgtat ttctagtacc tcagtccatg ggagagtttt tagattactt
     3841 gtgatgaggc ggaagacagc atccccattt gattaacctt cagggattaa tacatgtcat
     3901 ctttttgaat gagatgacaa tttcagcggc cttaattgtt agttctttaa ttatcagtta
     3961 ctctcttcta gagaacggac gaagcgatcg ctttcagttg actgctactg tagctggatt
     4021 aagcgcagga ctacgcatcg cccatctcaa gcccgacatc gaatgagccg gcgtacccat
     4081 gattggtctt tgcaggcttc aggatgagct gcattccacc ctttcgaaat atgccgtctt
     4141 cttcattttt tgttctggaa agtttcccgg gtttttgctg gcgttgcgca taagccggat
     4201 gctcgtgcac ccggtctgtt aaagcatcat cgaaataaag ctgcgacgtg aactcatagg
     4261 cccgcgcggt ttttggatca gtgcgaatct tgaaatgaat gtgaacggtt cggcctggat
     4321 accagccggg atatatcgtg aggaactgcg ctccgccgtc tgcatcggtc acctggtatc
     4381 cgcgcagaaa ttttttcccc atcgtattga aatggctatc gacgacgtcc gaatagatgc
     4441 cccgcgcgtc gcactgccag atgtcgataa tagcattcgc aatcggcctg cacgccgttt
     4501 tgcccactga aaatacatgc atgttgagcg cgagcagaac gccttctttt atcgcgccgt
     4561 cggaaggatc ggagcggata tccgcgcggc gcaggcgctc gtcgacgaaa tagggcccct
     4621 cggtctgttg aggggtgggg acgcacgaga acatttttgg acccgcctct ttctcgtccg
     4681 ctttaccgag tgaatatccg cttaccatat caagtcccgt tgctccgagg agccccagca
     4741 gctttcgtcg atccatcatc tacctcccga ttggcctgag atgcaattgc tgactgcgac
     4801 cggtaatttg cgcatcatta cccatgaccc tatgctgcgg atgaagaatc aggcaatcgc
     4861 ttctctccag cttcgacgat tgcgaatctt ttgagttcct gcgaggccca agcgtgcttc
     4921 cagcagtgag agccgatttc ttgaagagtt tgcctacacg tcgagcgcca gcttgttcat
     4981 gaggttgttt ttcaccgccg gccactcgtg ttcaagtatt gaatagacga ctgtgtcgcg
     5041 gatcgaaccg tcgcgcatga tttgatggct tcgcagtaca ccatcctgtt tggcgccaag
     5101 cctttcgatt gcggctcggg aaacctggtt gaaataatgc gtccggaact ccaccgcaat
     5161 ggcggctttt tgttcgaacg catatcgaag caataaaagc ttgctttcag tattcacact
     5221 cgttttgcag gccgatttcg cgtaccaggt gtaaccgagc gtagggcgac ggttgctttc
     5281 atccacgttg tagaagcgtg tggttcccac gatcttgttc g
//
LOCUS       DASSHJ010000019         7709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_532103, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000019 DASSHJ010000000
VERSION     DASSHJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532103"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFI43_01090"
     CDS             complement(<1..188)
                     /locus_tag="VFI43_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET7060754.1"
                     /translation="MDELLLQDLAGQAGRLLSEQGLMLATAESCTGGLLGDRVTSIAG
                     SSGWYDRGFITYSVVSKQ"
     gene            complement(294..911)
                     /locus_tag="VFI43_01095"
     CDS             complement(294..911)
                     /locus_tag="VFI43_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET7060755.1"
                     /translation="MSTFATDSSQPPGSGSPIPERDAGQKTEPARHVSLLPAHPSGDA
                     APRRVNRSFVRNHPAHFIAFGGGIGLSPAAPGTAGTLATLPLFWILNYFLAPQTLLML
                     VGAMFIVGVWACATTGRALGEHDHGGMVWDEMTAFCLVLLFTPDNVVWQSFAFLLFRL
                     FDIIKPPPIGYYDAKLRGGFGVMFDDILAAFYALLCLAAWKAFAA"
     gene            complement(889..1863)
                     /gene="thiL"
                     /locus_tag="VFI43_01100"
     CDS             complement(889..1863)
                     /gene="thiL"
                     /locus_tag="VFI43_01100"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET7060756.1"
                     /translation="MLSEFDIIQRYFSRPTSGSILGVGDDAALIKLGARMALAVSTDM
                     LVADRHFFPNADPRDLGYKSLAVNLSDMAAMGARPRWATLSLALPEAVVSAHSHWLEA
                     FADGFFTLAQEYQVDLIGGDTTQGPLNICVTIMGEVEAGKALRRSGAKPGDDIWHSGE
                     LGSAALAVAHKKGDIKLEAEDLAACLRALDTPMPRVALGQRLIGVAHSAIDISDGLVA
                     DLGHIVASSKVSGMIRMDEINCSPCMKSQLSEPVAARCLLGGGDDYELCFTASPSKRK
                     KIDALAQALDIPLARIGEIGEGEGLVVLDAGGNPLIVREKGYEHFRNG"
     gene            complement(2016..2531)
                     /gene="nusB"
                     /locus_tag="VFI43_01105"
     CDS             complement(2016..2531)
                     /gene="nusB"
                     /locus_tag="VFI43_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7060757.1"
                     /translation="MTSKRPDKPSGASGHAAGDSKGSPAQRPGRKTKRRAARELALQG
                     LYQWCVAGGTAESIEAQLRETDQFARADAEYLGQLLKGVLADAVELEAMIEPQLDRSF
                     KELSPVEISILLLGTHELKSRLEMPYRAVINEAVELAKTYGGTDGHKYVNGVLDKLAT
                     KLRTVEIPAKT"
     gene            complement(2528..3010)
                     /gene="ribH"
                     /locus_tag="VFI43_01110"
     CDS             complement(2528..3010)
                     /gene="ribH"
                     /locus_tag="VFI43_01110"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7060758.1"
                     /translation="MATYDNILEFEPDLDGGELRIGIVMSRFNMDVSDGLLSACTAEL
                     TKHGVQEANMLLVTVPGALEIPPALQKMALSDQFDALIALGAVIRGETYHFEIVANQS
                     ANGIAAVQLDTGIPIANGVLTTDTDDQAMARMSRKGMEAAQAAIEMANLLRGLEEATE
                     "
     gene            complement(3108..4235)
                     /gene="ribBA"
                     /locus_tag="VFI43_01115"
     CDS             complement(3108..4235)
                     /gene="ribBA"
                     /locus_tag="VFI43_01115"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7060759.1"
                     /translation="MVISSIQEIIAEIKTGRMVILVDEENRENEGDLLIAADFVTPSA
                     VNFMATHGRGLICLTLTEERCRQLNLPLMVAANRSPLGTNFTVSIEAASGVTTGISAA
                     DRARTVQAAVNPNAQPEDLVQPGHIFPLMAQTGGVLVRAGHTEAGCDLASLAGLAPAS
                     VICEILKEDGSMARLPDLVEFAAKHQLKIGAIADLIHYRSRTESLVKRVTERPIHTIH
                     GEFQLVAYLDKTANATHLALVKGEIDRETETLVRVHEPLSVIDLLDVGDETHSWNIND
                     TLHVIAEAGRGVIVLLHRRESAAELLERAAPSRSKPRIATATDLRDYGIGAQILKDLN
                     VGKMRLLAVPRKMPSMAGFGLEVTGYLERRGISRQPRHNLA"
     gene            complement(4244..4852)
                     /locus_tag="VFI43_01120"
     CDS             complement(4244..4852)
                     /locus_tag="VFI43_01120"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7060760.1"
                     /translation="MFTGIIEAVGEIRRVQSLEKGKSLSIASGSLDLSDTRIGDSIAV
                     NGVCLTVISLAEREFTVDASLETLNCTEGLDKPEKKVNLERALRLSDRLHGHLVSGHV
                     DGVGEVAGLQPSGESHIVTIEAPSFLSRYIARKGSITVNGVSLTVNRVSGARFEVNLI
                     PHTLVVTNLADLSMGTRVNIEVDMLARYVEQLLGVSRRVDKD"
     gene            5097..6362
                     /locus_tag="VFI43_01125"
     CDS             5097..6362
                     /locus_tag="VFI43_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7060761.1"
                     /translation="MFSDLSQSPLSKNLQRLFLLRNISVAAQCLTFALAYWVLEMQLP
                     WMEMVLVTVVLALLNLATWVRLRRNWPVSSVEFFAQLMVDVFALTALLYFSGGSTNPF
                     ISLYLLPLTIAAAALPWAYTWVMAAITISCYTVLLFYYIPLPHDHEEHNSEFNLHVSG
                     MWLAFVLSTVLIAWFVVKMGISIRERDKDLASAREQALRNEQLIALGTLAAGAAHELG
                     TPLATMAIVSGELQDEYTGDSEFQNNVRILRDQITQCKRTLTQLLADAGQARAEDASG
                     QAVDLFLRQVLDKWQLMRPSVKYTYHGAGVQPAPQILNTQLLSQSILNLLNNAADASL
                     KHVEIEVSWNHQELHLQILNHGEGLSPEAVQRAGQAFFTTKAPGQGFGIGLFLANANI
                     ERFGGRVRLTNREGGACTQVILPLIRQTV"
     gene            6359..6916
                     /locus_tag="VFI43_01130"
     CDS             6359..6916
                     /locus_tag="VFI43_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7060762.1"
                     /translation="MSMPADEIDDRPALLVVDDDLTFCGVLAKAMTKRGFSVTCAYSV
                     DQAMECAETCMPEYAVVDLRLPGTSGLTLVEKLKSLDPGTRIVMLTGYASIATAVEAI
                     KLGATHYLAKPVDIDDILMAFERTSGDADVPISAHPLSVGRLEWEYIQRVLNENKGNI
                     SVTAKMLNMHRRTLQRKLTKYPVKT"
     gene            complement(6972..>7709)
                     /gene="ribD"
                     /locus_tag="VFI43_01135"
     CDS             complement(6972..>7709)
                     /gene="ribD"
                     /locus_tag="VFI43_01135"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET7060763.1"
                     /translation="EAGIEVEAGLIEAQARELNVGFVSRMTRNQPWVRMKIAASLDGK
                     TALNNGTSQWITGDAARQDGHRLRARSCAILTGIGTVLADDPELSVRHVPTPRQPLRV
                     VVDSRLEIPVDAKLLRGSGELIFTANAAEGRIGALHDVGARVIVMPDQNNQNNRVDLA
                     NMMRTLADLGINEVLVEAGSALNGSLVSAGLVDELVIYFAPCLIGDAAQGMMKLPELT
                     DLSEKRGLKIRDFLSVGEDLRIIARFS"
BASE COUNT         1599 a         2307 c         2145 g         1658 t
ORIGIN      
        1 tcctgcttcg agaccaccga atacgtgatg aaaccgcggt cgtaccagcc cgagcttccc
       61 gcgatcgagg taacccgatc tccaagcaaa cctccggtgc acgactctgc ggtggccagc
      121 atcaacccct gctcggaaag caggcgtccg gcttgtccgg cgagatcctg aagcaatagc
      181 tcgtccattc attcctccct ggaattgatc atttcccgcc tttcccaaga atcagaaacg
      241 ccgtgccgct aggctccatg atagtcatgg aaccttcctg cttattcacc ctgctatgct
      301 gcaaatgctt tccatgcggc aaggcacagc agcgcgtaaa atgcggcaag aatatcatcg
      361 aacattacgc caaagccgcc gcgtagcttc gcgtcatagt aaccgatagg aggcggtttg
      421 attatatcga acagccggaa caggagaaaa gcaaaggact gccacacgac attatcggga
      481 gtgaacagca gcaccaggca aaaggcagtc atctcatccc agaccatgcc gccatggtca
      541 tgttctccga gcgccctgcc tgtcgttgcg catgcccaga cacccacgat aaacattgcg
      601 ccaacgagca tcagcaaggt ttgcggcgcg aggaagtagt tgagtatcca gaacaatggg
      661 agcgtggcaa gtgttcccgc ggttccgggc gcggcagggc tcagtccaat tccgccgccg
      721 aacgcgataa agtgtgcggg atggtttcgc acgaatgacc ggttgacacg acggggagca
      781 gcatcgccgg acggatgtgc aggcagtagc gagacatgcc tcgccggctc ggtcttctga
      841 cccgcgtcgc gttccggtat cggggaaccg gaacctggcg gctggctgct atccgttgcg
      901 aaagtgctca tagcccttct cccgtacgat aagcggattg ccgcctgcat ccagaacgac
      961 caggccctcc ccctcgccaa tttccccgat gcgggccaat ggaatatcga gcgcttgcgc
     1021 aagcgcgtcg attttcttgc gcttcgacgg cgatgcggta aaacagagct cgtagtcatc
     1081 cccgccgccg agcaggcatc ttgcagccac cggttcggaa agctggcttt tcatgcacgg
     1141 cgagcaattt atctcatcca ttctgatcat tccgctgact ttcgaagagg cgacgatatg
     1201 tccaaggtca gccaccagcc catcagaaat atcgattgcg ctgtgcgcga cgccaatcaa
     1261 ccgctgaccg agcgcgacgc gcggcatggg cgtatccagc gctctcagac atgccgcaag
     1321 gtcttccgcc tcgagcttga tgtctccctt cttgtgtgcg accgcaagcg cggcacttcc
     1381 gagctcgccg gaatgccaga tatcatcgcc tggttttgcg ccgctgcgcc gcagggcttt
     1441 tccagcttcc acctcaccca tgattgttac acagatatta agcggccctt gagtcgtgtc
     1501 gccaccgatc aggtctacct gatattcctg ggcaagcgtg aaaaagccgt ccgcgaacgc
     1561 ctccagccaa tggctgtgtg cagataccac cgcttccggc aatgccagcg agagcgtggc
     1621 ccatcttggc cgggcgccca tcgccgccat gtctgagagg tttaccgcaa gagatttata
     1681 acccaggtcc cggggatcgg cattgggaaa aaagtgccga tccgccacca gcatatcagt
     1741 ggacacagcc agcgccatcc tcgctcccag cttgattaaa gccgcatcat ctcccacacc
     1801 cagtattgac ccggacgtgg gacgggagaa gtatctctgg ataatgtcga attccgaaag
     1861 catcggcaaa cctgggcgcg gaagcaccgc gagggggagg ccccaatgtt accgcaaatc
     1921 cccaagaatg aggatgcaag aggatggggg taaaaggtgt ggaagacggc agggcggcga
     1981 gaataccgga aggatgcttt cgctatcgct tcgaatcagg ttttcgcagg gatctccacc
     2041 gtcctcagtt tggtcgcgag cttgtccagc acaccattca catacttgtg accgtccgtc
     2101 ccgccatacg ttttggcgag ctcgaccgct tcattgatga ccgcgcgata gggcatttcg
     2161 aggcggctct tgagctcgtg cgtccctaaa agcagaatcg aaatctcgac cgggctcaat
     2221 tccttgaagc tgcggtcgag ctggggctcg atcatcgcct cgagctctac cgcgtccgca
     2281 agtacgccct tcagcaattg accgaggtat tccgcgtcgg cccgcgcgaa ttgatcggtt
     2341 tcgcgcagtt gggcttcgat actttccgct gtgccccccg caacgcacca ttgataaagc
     2401 ccctgcaagg caagttcacg cgcggcacgg cgcttggtct tgcggcccgg cctttgagcg
     2461 ggagaaccct tcgaatcgcc cgctgcatgc cccgatgcgc cagacggctt gtcgggccgc
     2521 tttgacgtca ttccgtggct tcttcgagac cgcgaaggag attggccatc tcgattgccg
     2581 cttgcgcggc ctccatcccc ttccggctca tgcgcgccat cgcctgatcg tcggtatccg
     2641 tggtgagtac gccgtttgct atgggaattc ctgtatcgag ctgcaccgca gcgatcccgt
     2701 tggctgactg gtttgccacg atttcaaaat ggtaggtttc gccgcgtatg actgcaccaa
     2761 gggcgatcag cgcatcaaac tggtcggata atgccatttt ttgcagcgca ggcggaattt
     2821 ccagcgcgcc cggcaccgtg accagaagca tgtttgcttc ctgaacgccg tgttttgtca
     2881 gttcggcggt gcatgcgctg agtagtccat cactcacgtc catgttaaaa cggctcatga
     2941 cgatgccaat gcgtagctcg ccaccgtcaa gatcgggctc gaattcaagg atgttgtcgt
     3001 aggttgccat cgccgttctc cgaaaataaa tgcgcactgc gtggatcaca cttgctgctg
     3061 ctgcggagat ggaagatcaa aaccgcagat agcttcattt tagcggttta cgccaggttg
     3121 tgccgtggct gtctgctaat tccgcgcctt tcaaggtagc ccgtaacctc gagaccgaac
     3181 ccggccatgc tcggcatttt gcgcgggaca gccagcagcc gcatttttcc cacgttcaga
     3241 tccttcagta tttgcgctcc gatcccatag tcccgcaggt ctgtagccgt tgcgatacgg
     3301 ggcttggacc gggatggagc agcgcgctcg agtaactcgg cggcgctttc acgccggtgg
     3361 agcaatacaa tcacgccccg tcctgcctcc gcaatcacgt gtagcgtatc gtttatattc
     3421 caggagtgtg tttcatcccc gacatcgagc agatcaatca ccgagagcgg ttcatgcacc
     3481 cgtaccaggg tttcggtttc ccggtcgatc tcgcctttta ccaaggccag atgcgtcgca
     3541 ttggcagttt tgtcgagata ggcaaccagc tggaattcac cgtgtatggt atggatggga
     3601 cgttcggtca cgcgcttgac aaggctttcg gtacgactgc gataatggat caggtcggcg
     3661 atcgcaccga tcttgagctg atgctttgcg gcaaattcaa cgagatccgg aaggcgcgcc
     3721 atgcttccgt cttccttcag gatttcgcaa ataaccgaag caggggcgag acccgcaagg
     3781 ctcgccaggt cgcaacccgc ttccgtgtga ccggctcgga caagcacccc cccggtttgc
     3841 gccattagcg gaaaaatgtg gcccggctgg acaaggtcct ccggctgcgc gttcgggttc
     3901 actgcggcct ggaccgtccg cgcgcggtcg gccgcggaga tgccggtcgt gacgccactt
     3961 gcggcttcaa ttgaaaccgt gaaattcgtg ccgagcgggg agcggttggc ggctaccatc
     4021 aatggcaggt tgagttgccg gcaacgctcc tctgtcaagg tgaggcaaat caatccacgt
     4081 ccatgcgtgg ccatgaaatt gacggcggat ggggttacga aatccgccgc gatgagcaga
     4141 tcgccctcgt tttcgcggtt ttcctcatcc acgaggatca ccattctgcc ggtcttgatc
     4201 tccgcgatta tttcctgtat cgaactgatg accatattaa attttagtct ttgtccacac
     4261 gtcgagaaac gcctagcaat tgctcgacgt agcgggcaag catatccact tcgatattca
     4321 ccctcgtgcc catgctgagg tccgccagat tggtcacgac cagcgtatgg ggaataagat
     4381 tgacttcgaa cctcgctcct gaaacgcgat taacggtcaa gctgacgccg tttaccgtga
     4441 tcgacccttt gcgggcaata tatctcgaca ggaaggacgg cgcctcgatg gtcacgatat
     4501 ggctttcccc tgacggctgc agcccggcca cttcgccgac cccgtccaca tggccgctca
     4561 cgaggtgccc atgaagcctg tcggatagac gcaaggctct ttccaggttg accttctttt
     4621 ccggcttgtc gagcccttcc gtacagttca gcgtttcgag cgatgcgtcg accgtgaatt
     4681 ctctttccgc aagagatatc accgtgaggc aaacgccgtt cacagcgata ctatccccta
     4741 tcctggtatc gctcaagtcc agcgaaccgc tggcgatact caagcttttt cctttctcga
     4801 gtgactgaac gcgccttatt tctccaactg cctcgataat cccggtaaac atcaatgtct
     4861 tccctttccg ctacaatact ctgccatcag ccaggcaggg gcgcactgcg gcaatatgtc
     4921 gcatggcgtt cagctgaaat atgcgctacc gttcgcggac ctacattcaa gggaacccgc
     4981 ggacgaccgc ctgccgaagg acgagtccat gggggaaccg acggaagaat tccatggcgc
     5041 acgtccagat tcctcattta atatacgaag cagccagccc cgaacgcccg agaggtatgt
     5101 tcagcgatct cagccagtcg cccctgagca aaaatctgca acgactgttt ctgctccgaa
     5161 atatttcggt tgccgcacaa tgcctgacgt ttgcgctggc gtactgggtg ctggaaatgc
     5221 agttgccctg gatggaaatg gtgctggtga cggtcgtgct ggcactgctc aacctggcaa
     5281 cctgggtacg gctgcggcgc aactggcctg tctccagcgt cgaattcttc gctcagctga
     5341 tggtcgatgt cttcgcgttg acagccttgc tgtatttcag cggcggctcg accaacccct
     5401 tcatttcact ctatctgctg ccgctgacga ttgctgcggc agccctgccc tgggcttata
     5461 catgggtgat ggcggccatc acgatcagct gctacacggt gctgctgttt tattacatac
     5521 ccctgccgca cgaccatgaa gagcacaaca gcgaattcaa cctgcatgta tctgggatgt
     5581 ggctcgcctt tgtcctgagc actgtattaa tcgcatggtt cgttgtcaag atggggatat
     5641 cgatccgcga acgcgacaag gacctggcgt cggcgcgtga acaggcgttg cgcaacgaac
     5701 aactcatcgc cctgggcacg cttgctgccg gcgcggcgca tgaactgggc acgccgctcg
     5761 caaccatggc gattgtatcc ggagaactgc aggacgaata tactggcgac agcgagtttc
     5821 agaataatgt caggatactt cgcgatcaaa tcacgcagtg taagcgcaca ctgacccagt
     5881 tgcttgcgga tgcgggacag gcacgcgcgg aagatgccag tggacaggcc gtggaccttt
     5941 tcttgcggca ggtactggac aagtggcagt tgatgcggcc ttccgtcaaa tacacttacc
     6001 acggggccgg ggttcagcca gcgccccaga ttctcaatac ccagttattg agccagtcga
     6061 tactgaatct tctcaataat gccgcggacg cgtctttgaa gcacgtcgag atcgaggtga
     6121 gctggaatca ccaggaattg catctgcaaa tcctgaatca cggtgaagga ctttctccgg
     6181 aggcggtgca gcgcgccgga caagcctttt ttaccactaa ggcgcccggt cagggattcg
     6241 gcatcggcct gtttctggcg aatgcgaata tcgagcgttt tggaggcaga gtccgcttga
     6301 ccaaccgtga aggcggggcc tgtacacaag tgattttacc cttgatacgg caaacggtat
     6361 gagcatgccc gcggatgaaa tcgacgaccg gccggcactg ctggtcgtcg atgacgacct
     6421 gaccttttgc ggcgtcctcg cgaaagccat gacaaagcgc ggattcagcg tcacttgcgc
     6481 gtattccgtc gatcaggcga tggaatgtgc ggaaacctgc atgccggaat atgcggtggt
     6541 cgacctgcgc cttcccggaa cttcgggtct gaccctggtg gaaaagctca agtcgctcga
     6601 tcccggcacg cgcatcgtca tgctgaccgg ttatgcgagc attgccactg cggttgaagc
     6661 gatcaaactc ggcgcgaccc actacctggc caagccggtg gatatcgatg atattctcat
     6721 ggcattcgag cgcacgagcg gcgacgcgga tgtgccgatc agcgcacacc ccctctcggt
     6781 gggacgtctg gagtgggagt atatccagcg tgttttgaac gagaacaaag ggaatatttc
     6841 ggtgactgcc aagatgctta acatgcatcg ccgcacgctg cagcgcaagc ttaccaagta
     6901 cccggtaaaa acctgaggct atggagactt ggagcctcgc agcctcgtgg ctcccctgaa
     6961 aatatttgtc actatgaaaa gcgggcgata atgcgtagat cttcgcccac gctgagaaag
     7021 tcgcggattt tgaggccacg cttttccgac aggtcggtca actccggcag cttcatcatt
     7081 ccctgggctg catccccgat caggcatggc gcgaaataaa taaccagctc atcgacaagt
     7141 cctgccgaaa cgagggagcc gttcagcgca cttcctgcct ccaccagtac ctcattgatc
     7201 cccagatcgg cgagcgtacg catcatgttc gcgaggtcga cgcgattatt ctggttattc
     7261 tgatcaggca tcacgatgac gcgcgcgccc acgtcatgca gcgcgccgat cctgccctcc
     7321 gcggcattgg cggtaaagat cagctccccc gagccgcgca gcagcttcgc gtccacgggg
     7381 atttccaggc ggctgtcgac cacaacgcgc agcggttgcc ttggcgttgg tacatggcgc
     7441 accgaaagct ccggatcgtc ggccagtacg gttccaatcc ctgtgagtat tgcgcaggaa
     7501 cgcgcgcgca ggcgatggcc atcctgcctg gccgcgtcgc cggtaatcca ttggctcgtg
     7561 ccgttgttga gcgcggtctt gccgtcaaga cttgcggcaa tcttcatgcg aacccagggc
     7621 tggttgcgcg tcatgcggga gacaaaaccg acattcaact ctcgcgcttg agcctctatc
     7681 agaccggcct cgacctcgat cccggcctc
//
LOCUS       DASSHJ010000020         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_969865, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000020 DASSHJ010000000
VERSION     DASSHJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969865"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFI43_01140"
     CDS             <1..902
                     /locus_tag="VFI43_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7060764.1"
                     /translation="LPNWDVTVGARQEWWSAMNGVVGSTAVPDRAVSALSPKVSIGYE
                     PGQWKVRYSFGRAHRFPVIAELFQSLSTPTSILTANALLKPENGTHHNLMVEYGLPRG
                     YIRVNIFRDDIKDAIQQVQRVSGVLTSSAFQNIDQTSTTGVELIFDQRRIMGSRFDFT
                     FNGTWMNAKVDKGGTVAFTDPTGANADYSLDGKQRIRLPHWRANFFATYNATEAWDIS
                     LSGRYTSDSFNDPDNRDHVNNVFGAQSSFFFMDFKTSYRYKFQNGLKSRFSFGITNLN
                     NDKAWVFHPYPQRTYLVEAAFSY"
     gene            complement(949..1779)
                     /gene="serB"
                     /locus_tag="VFI43_01145"
     CDS             complement(949..1779)
                     /gene="serB"
                     /locus_tag="VFI43_01145"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HET7060765.1"
                     /translation="MNLIIQGLDIQNSDLRALAKLSGADQIEQITGEAFRLKNANQRE
                     HVPNYCAEAELDFAFVDPSRQLSDFGLVAMDMDSTLLAIESIDEVADMHGIKPQVAEI
                     TQRTMRGEIIFEESLRQRTALLQGLDEHALEEVYNQRVRLSPGAERMLHQMKKAGVRT
                     MVISGGFTFFTDRIKSRLDFDYTAANRLEIVDGKLSGSVLGKIIGAEGKAEALIKVRE
                     ELGLQKDQVIAIGDGANDLRMMAEAGVSVAYHAKPIVQENATYALNHVGLDGVVNLFA
                     "
     gene            1960..>3139
                     /gene="purT"
                     /locus_tag="VFI43_01150"
     CDS             1960..>3139
                     /gene="purT"
                     /locus_tag="VFI43_01150"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HET7060766.1"
                     /translation="MNHKSRAETPLLGTPLSPAATKVMLLGSGELGKEVIIALQRLGI
                     ETIAVDRYPNAPGHQVAHRSHVIDMADGAALRALVERERPQVIVPEIEAIDTAMLMEI
                     ERLGWARVIPTARAAHLTMNREGIRRLAGETLALPTSRYAFANSLEELHFAIKNGVGY
                     PCVIKPVMSSSGKGQSKVDSVQEVEAAWDYAASAARVNQERVIVESFIAFDYEITQLT
                     VRAAGPDGAVETHFCEPIGHVQVQGDYVESWQSQEMSPLALQSARNIAGKVTDNLGGT
                     GVFGVELFIKGDEVWFSEVSPRPHDTGMVTMCSQVQSEFELHARAILGLPVDTTLREP
                     GASAVIYGRLEEQAIAFAGVADALRVPRSDVRLFGKPVSFLKRRMGVALANGSDTNEA
                     R"
BASE COUNT          690 a          860 c          823 g          766 t
ORIGIN      
        1 ttctcccgaa ctgggacgta acggtcgggg cgcggcagga gtggtggtcg gcgatgaatg
       61 gtgttgtggg aagcaccgcc gtgccggacc gcgcggtttc cgccctttca cccaaggttt
      121 ccatcggcta tgagcctgga caatggaagg tgcgttactc attcggccgc gcgcatcgat
      181 ttccggtaat tgccgagctg tttcaatcgc tgagcacgcc caccagtatc cttaccgcca
      241 atgctctgct caaacccgag aatggcacgc atcataacct tatggtggaa tacggcttgc
      301 ccaggggcta cattcgcgtg aacatcttcc gcgatgatat caaagatgct atccagcagg
      361 tacaacgggt ttcgggcgtc ctcacttcaa gtgctttcca gaacattgat cagaccagca
      421 cgacgggtgt ggagctgatt tttgatcaac gccgtatcat ggggtcgagg ttcgacttca
      481 ctttcaacgg aacctggatg aatgcgaaag tggataaggg cgggaccgtg gcattcacgg
      541 acccgactgg ggcgaacgca gattatagcc ttgatggaaa gcagcgtatc cgcttgccgc
      601 actggcgcgc aaatttcttt gccacctaca atgctaccga agcatgggat atatcgctct
      661 ctggccgtta taccagcgat tccttcaacg atcccgataa cagggatcac gtcaataacg
      721 tgtttggtgc acagagttcg ttctttttca tggacttcaa aactagttac cgctacaagt
      781 ttcagaatgg gttaaaaagc aggttctcat ttggaattac caatctcaac aatgacaagg
      841 catgggtttt tcatccctat ccgcagcgca cctacctggt tgaagccgct ttcagctatt
      901 agccattctt gcaagggcgc aaactgaata cagcttccac agcaaaattc atgcaaacag
      961 gttgaccact ccgtcgagtc ccacatgatt gagtgcataa gttgcgtttt cctgcacgat
     1021 gggcttagcg tgataggcga cgctgacacc ggcctcggcc atcattctca ggtcgttcgc
     1081 gccgtccccg atggctatga cctggtcttt ctgcaaaccc aactcctccc gtacctttat
     1141 taatgcttcc gcttttccct ccgcgcctat gatttttccc aagacgcttc cactaagctt
     1201 cccatccacg atttcaagcc tatttgccgc ggtatagtca aaatcgagcc tgctctttat
     1261 ccggtcggta aaaaatgtaa atcctcccga gataaccatt gtcctgacac ctgctttctt
     1321 catctgatgc aacattcttt ccgcgcctgg gctcaatcgg acccgctggt tgtacacttc
     1381 ctcgagcgcg tgctcgtcaa gcccctgcag aagcgctgtg cgctgcctca agctctcttc
     1441 gaagatgatt tcgccgcgca ttgttcgctg cgttatttcc gccacctgcg gcttgatccc
     1501 gtgcatatcc gctacttcgt caatggattc gattgcgagc aaggtggagt ccatatccat
     1561 cgccaccagg ccgaagtctg aaagctgccg cgatggatcg acgaaggcaa aatccagttc
     1621 agcttctgca caatagttcg ggacatgttc acgctggttc gcgtttttca accggaaagc
     1681 ctccccggtg atttgctcga tctgatcggc tccagagagt ttcgccagcg ctcgcaagtc
     1741 gctgttttga atgtcgagcc cttgaatgat gagattcata tacaatcctt cagttgggaa
     1801 agcgtccgaa ttgtgccatt aattgctgag ttcttccata cgtggtttgt tttatccgct
     1861 aacgctttcc ggattcgatt cgtaacgcaa ttgatgctgt ttcaaaatgt tcagcatgcg
     1921 cttgaacctt gcctcgatcc tgtcgtgttc cataaaagca tgaaccataa atcgcgcgcc
     1981 gaaactcccc tgcttggcac ccccctcagt cctgctgcga cgaaagtgat gctgctggga
     2041 agcggcgagc ttggcaagga agtcatcatt gcattgcagc gcctgggcat tgaaacaatc
     2101 gcagtggatc gttaccccaa cgcccctggt catcaggtcg cgcaccgctc gcacgtcatt
     2161 gatatggctg acggcgctgc attgcgcgca ctggtagaac gtgagcgccc gcaggtaatc
     2221 gttccggaga ttgaagcgat cgatacggcc atgctgatgg aaatagagcg tctgggatgg
     2281 gctcgggtca ttcccacagc tcgtgccgcg cacctgacga tgaatcgtga aggtatccgc
     2341 cgacttgcag gcgaaacact tgctttgccc acctcgcggt acgcattcgc aaattcgctt
     2401 gaagaactcc acttcgcgat aaaaaatggc gtcggctatc cttgcgtgat aaagccggtg
     2461 atgtcttcat caggcaaagg tcaatcaaaa gtcgacagcg tacaagaagt cgaggcagca
     2521 tgggattatg cggccagcgc ggcacgagtg aaccaggaac gcgtgatcgt ggaatctttt
     2581 atcgcgttcg attacgagat cacccaactc accgtacgcg cagctggacc cgacggcgcg
     2641 gttgaaacgc atttctgtga gcctatcggg catgttcagg ttcaaggtga ttatgtggaa
     2701 agctggcagt cacaggaaat gtctcccctg gcgttacagt cggcccggaa cattgctgga
     2761 aaggttaccg ataatctcgg tggcacgggg gtttttggcg tcgagctatt catcaaggga
     2821 gacgaggtgt ggttcagcga ggtcagtccc cgtccccatg ataccggcat ggttaccatg
     2881 tgcagccagg tccagagcga atttgaactg catgctcgcg caatactggg tttgcctgtc
     2941 gataccacgc tacgtgaacc cggtgcgagc gcggtgattt atggccgatt ggaggagcag
     3001 gccattgctt tcgctggcgt cgccgacgcg cttcgcgtac cgagaagcga cgtgcgcctg
     3061 ttcggcaaac ctgtttcatt cctcaagcgc aggatgggtg ttgccctggc caatggaagt
     3121 gacacgaatg aagcgagat
//
LOCUS       DASSHJ010000021         6188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1622530,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000021 DASSHJ010000000
VERSION     DASSHJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622530"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            831..983
                     /locus_tag="VFI43_01155"
     CDS             831..983
                     /locus_tag="VFI43_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060767.1"
                     /translation="MTKYYSQNRPDNPGQPTKPDEPDKPVDPRPFDPDPSPGDPIVDP
                     ADEPLL"
     gene            complement(1376..2797)
                     /locus_tag="VFI43_01160"
     CDS             complement(1376..2797)
                     /locus_tag="VFI43_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET7060768.1"
                     /translation="MAAHPGYAGIHPLPHALDSFAARVLLIRAAERSLDLQYYTWQKD
                     MTGTLLFEELHEAAARGVRVRLLLDDNNTSGLDSTFATLDAHPNIEVRLFNPFAIRGL
                     RVFKYIFDFSRANRRMHNKSFTADNQVTIVGGRNIGDQYFGAGGGVLFADLDIMAVGP
                     VVDAVSKSFDRYWASESSYPVANLIPRADSDTIAEVTAAVSRVKRDPATTVYTEAIRN
                     SPFIRDLSSNQLDLEWATTRVISDDPAKGLGQAAPEDLMIHTLKEIIGEPASEVDLVS
                     PYFVPTAAGTASFTALAERGVQVQVLTNSLEATDVALVHAGYAKWRKRLLEAGIKLYE
                     SRHLAPRVRRSGSGFGSGPDSDGFGSKGSSDTSLHAKTFSVDESRVFVGSFNFDPRSR
                     ELNTEMCFVIDSPVLAGHIADAFQHTIPDNAYELGLSNKGELYWIERVQGKEVRHDVE
                     PGTNFLQRAALRMMSLLPIDWLL"
     gene            complement(2970..4085)
                     /locus_tag="VFI43_01165"
     CDS             complement(2970..4085)
                     /locus_tag="VFI43_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7060769.1"
                     /translation="MREIRAAVVREKGGPFSVENLLMDEPYPEEVLVRVIATGMCHTD
                     MVARDQLYDVPLPIVLGHEGAGIVEHAGSSVKKVAAGDHVVLTFMWCGHCNPCLRGDL
                     TYCENFYPLNFGGAREDGSTSTYEDEGRRKPLHDHFFGQSSFGTFALVHERNVIKVPK
                     DVPLELLGPLGCGIQTGAGAVLNALKVSPGSSFAVFGGGAVGLSAVMAARAAGAAIII
                     VADVVPSRLTLAKDLGATHMVNSRESDAVAAVREITSEGANFTLESSGRPEVLRQAID
                     ALAIRGTCAIVGAPALGTEASFDVNGVMTTGKRIIGVIEGDSTPDLFIPSLVELYKLG
                     RFPFDKLVKFYTLEQINQAAEDSEKGVTIKPIIKLLS"
     gene            complement(4330..5874)
                     /locus_tag="VFI43_01170"
     CDS             complement(4330..5874)
                     /locus_tag="VFI43_01170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7060770.1"
                     /translation="MADKYIALPKGVSERTFDSAISDFRQALGDPSVLTKAEQLAPYV
                     KTMMPVPDSEHMPSAALMATTVEQIQKIVGICNRYKVPIWPISTGKNLGYGSAAPAER
                     GQVVLDLKRMNRILEIDKDLAFALVEPGVTYKQLYDHIAKHDLGLWLSVPAPSAIAGP
                     TGNTLDRGVGYTPYGEHFMFSCGMEVVLANGEVLRTGMGSLPNSNTWQVFKWGYGPYL
                     DGIFTQSNYGIVTKFGFWLMPAPPVYKPFTVQYQNDEDIVEIVETIRPLRINNVIPNA
                     AVIAYALYDAPTKMRRGDYFTGPGAIPDAAVRKIAKDHNFGIWNVYAALYGTQEQVDV
                     NWKIVTQAFGRSGKATILTEKEMAGDRTFNYRSEIMRGGMNLVEFALYNWRGGGGSMW
                     FAPVSQARGSETLKQMALTKQILAKHGLDYSGEFIVGMRDMHHIVDVLYDKTNPEQVK
                     AAYQCFDELLTVFSDKGYGTYRVNTAFMDKVAHTYGPVQRHVHKMLKDALDPNGIIAP
                     GKSGIR"
     gene            complement(5900..>6188)
                     /locus_tag="VFI43_01175"
     CDS             complement(5900..>6188)
                     /locus_tag="VFI43_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060771.1"
                     /translation="KSPGGWGPLPTAADQNQTAKNGTAADQGRRAVNWRPENWVEATG
                     YTVAAAAFGLGKASETCSQRAFVGRPQRDDAGQQKGRTFQGERFVSLILDT"
BASE COUNT         1382 a         1737 c         1687 g         1382 t
ORIGIN      
        1 aaaaagggca gcaatatcca cccgaatgcc agggagacga caattaacag gagcaggaag
       61 gctttttgct gaagctggag gggagttcat agatttgtgt gcttttcaat tatcggaatt
      121 gttatttgac atgaaaccat attagtatgc tatcgaaccg aggaattaaa aagcggagag
      181 tagtattaac acagcaattc cgctggagca aattaaggag cagtcatgaa gttttcctct
      241 aaatacgcaa gtcggtcagt ggcactcgca atagttattg gaagcgccct tggtttcagc
      301 acgggcgtaa cctttgccca atctggcacc ggacaagata gctccactgg acagggtagt
      361 tctgggtccg gcagcgctgc aaagccatcg aaggagaacc agggaatgga gtcagggaca
      421 gtagatcaaa acaaaaggaa tgagcgtaac aagagtatag aaccacgcgt ccctacggta
      481 cgtaaagaca agcaaggggc cccgaacaaa gaccccagcg atattcaagg cgggcctatc
      541 gggccaaatt aggcacctaa tatcggcatc aagcggtatc gacaccatag ctgaacttct
      601 tgccgagagg cgattggtaa cttctcgccg ctggattacc gttcacggat gcctgcccca
      661 gcaaatgact cattctttat accactgacc aaccgcgcaa ttaggtgacg ggagtgtaag
      721 gtaccttact gaatacaagg ttcgctaacg tacggaacta tttgatgctc agcgtattat
      781 tgaattgttc cccgaaaagt gggcaattat ctatttcatg gaaaagcctt atgacaaaat
      841 attattctca aaaccgccct gataaccccg gtcagcccac caagcctgat gagccggata
      901 aaccggtgga tccccggccc tttgatcccg atccgtcgcc cggtgatccg atcgtggatc
      961 ccgcagacga gccgctgctc tgactttgcg tctgctttcc aggcgcgtta ataccctgca
     1021 ggggattttt tgatttaagc atcccatgat aggcatcgat aggtgtctta gacttaactg
     1081 gtttagtgga aggttgtcgc gaatttcgga tactgatggt aacggtaaag actggttact
     1141 ggagtaagaa atgtacaaga ttctgccccc gtcacatgac gtgatggagg attattctaa
     1201 ttgaaccggt acaggtagac gtaggtgtcg acaagcgtag cgcgacgtca taaggcagag
     1261 tcgtttcgtg agggttttct gcgaggcaat aacccaatct ctgcaatata atcctagacc
     1321 ccgaatggcg gaagtaaaca agtgtggctg aacgaaaggt atcaacaggc agtcctcata
     1381 gcagccagtc tatgggcagc aacgacatca tcctgagagc cgctcgttgc aggaaattcg
     1441 tgcccggctc cacatcatgc cgaacctctt ttccttgcac gcgttcgatc cagtagagtt
     1501 cgcctttgtt ggacaggcca agctcataag cattgtcagg aatggtatgc tggaatgcat
     1561 cggcgatgtg cccggccagt accgggctgt cgatgacaaa acacatctcg gtattcagct
     1621 ccctggagcg agggtcgaaa ttgaacgacc cgacgaaaac tcgcgattca tccacggaga
     1681 atgtcttggc gtgcaggctg gtatcagaac tgcccttcga cccgaatcca tcggaatccg
     1741 ggccagaccc aaatccagat ccagatcttc tcactcgcgg cgccagatgc cgagattcgt
     1801 agagcttgat gcccgcctca agaagccgct tgcgccactt ggcgtatcca gcatggacta
     1861 gcgcaacatc ggtggcttcc agcgagttgg tgagcacctg gacttgaacg cctcgttcgg
     1921 ccagcgccgt gaatgaagcc gtgcccgctg cagtgggtac gaaatagggc gacacgagat
     1981 cgacttcgct ggcgggttct ccaatgattt ccttcagcgt gtggatcatc agatcctcag
     2041 gcgcagcctg gccaagtcct ttcgcaggat catcgctgat cacccgggtg gtagcccact
     2101 caaggtcaag ctgattgctg ctcaaatccc gtatgaaggg cgaattgcgt atcgcctcgg
     2161 tataaacggt agtggccggg tcacgtttga cgcgcgacac agcagcggtg acctccgcga
     2221 tcgtatcgga atcagcccgc ggaattaaat tcgcgacagg atacgaggat tcgctggccc
     2281 agtagcggtc aaaagacttt gataccgcgt ctaccacagg acccaccgcc ataatgtcga
     2341 ggtcagcaaa caagactccg ccgccggcgc cgaaatactg atccccgata tttcggccgc
     2401 caacgatcgt gacctggttg tccgcagtga aggacttgtt gtgcatgcgt cgattagcgc
     2461 gcgagaagtc gaaaatatac ttgaagacac ggaggcctcg tattgcaaac ggattgaaca
     2521 accgcacttc aatattcggg tgagcatcga gcgtggcgaa cgttgaatct agcccggatg
     2581 tattattgtc atccagcagc agccgcacac ggacgccacg cgccgcagcc tcatggagtt
     2641 cttcgaacag cagcgtccca gtcatgtctt tctgccaagt gtaatactgg agatcgagcg
     2701 aacgctcggc agctcgtatc aatagcacgc gtgccgcgaa ggaatcgagt gcgtgaggca
     2761 atggatggat tccggcgtag cccggatgag ctgccacccg cggcgtgcct tcaccgatac
     2821 cggcaatacc attttgggaa aggcggttgc gatgtgcgat gggtttcccc tgcgaaaact
     2881 cgtttcagcc gtgcgaaggg tcagaacaag cagacttgcc cgcgggattg caagtggaat
     2941 tgttttacca ggggcttacg gctgaagtct tacgacagta gcttgatgat cggcttgatc
     3001 gtcacaccct tctcgctatc ttccgccgcc tggttgatct gctcgagtgt atagaatttg
     3061 accagtttgt cgaaggggaa ccgtccgagc ttgtagagct caaccagcga aggtatgaag
     3121 agatcaggag tgctgtcgcc ttcgatgacc ccgataatgc gcttgcccgt ggtcatgacg
     3181 ccatttacgt cgaagcttgc ctcggtaccc agcgcgggcg ctccgacaat cgcgcatgtg
     3241 ccgcgtatgg caagcgcgtc gatagcctgg cgcagcactt ccggccgacc gctcgattcg
     3301 agcgtgaaat tcgcgccttc actggtgatt tcgcgaaccg ccgcaacggc atcgctttcg
     3361 cggctgttga ccatgtgcgt ggcgcccaag tccttcgcta gcgtcaggcg cgagggcacg
     3421 acgtcggcaa cgatgatgat cgcggccccg gcagcgcgtg ccgccattac tgcgctcaaa
     3481 cccacagcgc ccccgccgaa tacagcgaaa ctgctgcctg ggctcacttt cagggcgttg
     3541 agcactgcgc cggccccggt ttgaatgccg caacccaagg gcccgagcaa ttcgagaggc
     3601 acgtctttgg gcaccttgat gacgttgcgc tcgtgaacga gcgcaaatgt tccaaatgac
     3661 gactgcccga agaaatggtc gtggaggggt ttgcgccggc cttcatcctc gtaggtcgat
     3721 gtgctgccgt cttcgcgcgc gccgccaaag ttaaggggat agaagttctc gcaataggta
     3781 aggtcgccgc gtaggcaagg gttgcaatga ccgcaccaca tgaaggtcag gacgacatga
     3841 tcacccgccg ccactttttt tacgctgctg cctgcgtgct cgacaatgcc cgcgccttca
     3901 tgcccaagca caatgggtag cggcacgtca taaagctgat cccgtgcaac catatctgta
     3961 tggcacatgc cggtcgcgat cactcgcacc agcacctcct ccggatatgg ctcgtccatc
     4021 agcaggtttt ctacactgaa ggggcctcct ttctcacgaa ccaccgctgc cctgatttcc
     4081 ctcattctga atctcctgct gggtctctct catcaaaagc ttgtcttcga tacaccgcca
     4141 tttccgcgga agcggcaatc tatcgctttg aaccttaccg ttacattgca aatctggata
     4201 cctgctttcg cgggtatgat gcatacaaca aactcagcca gcatttcatc tacgggcctc
     4261 taccataagg agagaaggca ttgatgaggt tccagcctca tgtcagcacg tcacgggcta
     4321 ggtctttccc taacgaattc ccgacttgcc gggggcaatg atcccattcg ggtccagagc
     4381 gtccttgagc atcttgtgca catggcgctg caccggcccg taagtatggg cgaccttatc
     4441 catgaaggcg gtatttaccc ggtaggtacc gtagcccttg tcggaaaaga cggtcagcaa
     4501 ctcgtcgaag cattggtatg ccgccttcac ctgttccggg ttggtcttgt cgtatagcac
     4561 atcgacgata tgatgcatat cccgcatccc gacgatgaac tcgccggaat aatccagtcc
     4621 gtgcttggcc aggatctgct tggtaagcgc catctgcttc agggtttcgc tgccccgcgc
     4681 ctgcgagacc ggggcaaacc acatcgagcc tcctccgccg cgccagttgt acagtgcaaa
     4741 ttccacgagg ttcattcccc cgcgcatgat ctcggagcgg taattgaagg tccgatcacc
     4801 cgccatttcc ttttccgtca ggatggtcgc cttgccggac cgcccgaacg cctgggtcac
     4861 gatcttccag ttcacgtcaa cctgctcctg ggtgccatat agcgctgcgt agacgttcca
     4921 gataccgaaa ttgtgatctt tggcaatttt ccgcaccgcc gcatcaggaa tcgcccccgg
     4981 tccggtgaaa tagtcgccgc gccgcatctt cgtcggggcg tcatacagcg cgtaggctat
     5041 taccgctgcg ttcgggatta cgttgttgat acgcaacgga cgaatcgttt caacgatctc
     5101 gacgatatcc tcgtcattct ggtactgaac ggtgaatggc ttgtacacgg gcggtgcagg
     5161 catgagccag aagccgaact tggtgacgat gccgtaattg gattgggtaa agatgccgtc
     5221 gaggtagggg ccgtagcccc acttgaaaac ctgccaagtg ttggagttcg gcagcgaccc
     5281 catgcccgtg cgcagaactt ccccgtttgc caacacgacc tccattccgc atgagaacat
     5341 gaagtgttct ccatagggcg tatagccgac tccgcgatcc aacgtattgc cggttgggcc
     5401 cgcgattgcg gagggcgcag gcaccgacag ccacaagcca aggtcgtgct tcgcgatatg
     5461 gtcgtacaat tgcttatagg tcacgccggg ttcgaccagg gcaaaggcaa ggtctttgtc
     5521 gatttcaaga atgcggttca ttcgcttgag atcgagaacc acctggcccc gctcggccgg
     5581 cgctgcggac ccgtagccca ggtttttccc tgtggaaatg ggccagatcg gcactttgta
     5641 gcgattgcag attcccacta tcttctggat ctgctccaca gtcgtcgcca tcagagctgc
     5701 ggaaggcatg tgctccgaat ccggcaccgg catcatagtt ttgacgtagg gggcgagttg
     5761 ttccgctttt gtcaggacgc tggggtcacc gagcgcctgc cgaaaatcac tgattgcgct
     5821 gtcgaacgtc ctttccgaaa ccccctttgg aagcgcaatg tatttatcgg ccatgctcga
     5881 ccactccctc cgcgcgtgat taggtgtcta atatgaggga gacgaagcgt tctccctgaa
     5941 acgttcgtcc cttttgctga cctgcgtcgt cgcgttgagg ccggcctaca aacgcgcgct
     6001 gggaacaagt ttcactcgcc tttcccaggc caaatgccgc cgcggcgacc gtataaccgg
     6061 tcgcttcaac ccagttttcg ggcctccagt tgactgcgcg gcgcccctgg tctgcggcag
     6121 ttccattttt ggctgtttgg ttttgatctg ctgccgtggg aagcggaccc catccacccg
     6181 gtgatttc
//
LOCUS       DASSHJ010000022         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_640501, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000022 DASSHJ010000000
VERSION     DASSHJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640501"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2606)
                     /locus_tag="VFI43_01180"
     CDS             complement(<1..2606)
                     /locus_tag="VFI43_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7060772.1"
                     /translation="MNLSELCIRRPVMTMLLSASAVLAGVIAYGGLPIAALPSYDLPT
                     ISVTAALPGASPEIMSSSVATPLERQFSSISGLSLISSSSTLGNTSITLEFEQDRDID
                     AAAVDVQAALLRAQRALPVEMTSPPSYRKVNPADAPIIFLALTSPSMSLSELNSYAED
                     LISPTLSTLTGVALVQINGRKKYAVRVRANPEALAARGLTLDDVAAAIRTANANTPIG
                     TLEGPRQTLTIQANRQLSNAAQFAKLIVATSENGSPVRLSEVADVEDSVESVKTASWV
                     NGERAITLSVQRQPGANTVATVDAIKATIPTLTAQMPSSVQLKLTSDRSESIRHSIHD
                     VKVTLGITILLVLLIIFLFLRKAYATLIPALTLPISLLGTVAVMRVLDYSLDNISLLA
                     ITLAVGLVVDDAIVMLENIVRHIERGLSPLQAALLGSREVSFTIISISLSLVAVFIPI
                     FFMPGVIGLLFHEFAAVVGIAVLMSAVVSLTLVPVMTSRLMTGHQGESLSLRWTEWFE
                     RGFERTSRFYERMLDWSLRNRRVVLGIAAATFVLTGVLFTVLPKGFFPTEDIGQALVT
                     VEAVEDISFPAMAELLQRTGSVIRANRAVDTLIVNASDTNSARLFMTLKPRSERPHLD
                     KVMESLRRDVRVIPGVNVFITPIQNLRLGGRTSKSRYQYVMRSVQPAELRVGAEGLMA
                     KMREDKVFRDVTNDSQLKGLQVRLKINRDKVDLLGVPMADIRSTLYSAFGERQVSTIY
                     TSTDSYQVILQVTPEARADESAFGKIYLRGKDDMLVPLSSVASVEREVGPIAINHVGQ
                     LEALTVSFNLAPGASLGDASARIEQFKQELNMPASILTSYTGDAAAFQSSQASQVILI
                     VAALLVIYILLGV"
     gene            complement(2620..>3318)
                     /locus_tag="VFI43_01185"
     CDS             complement(2620..>3318)
                     /locus_tag="VFI43_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7060773.1"
                     /translation="RIARGFSEITAPIAGRTGAVSVYQGSLVQPNDVLVNITQIDPIN
                     VSFTLSEREFVPLQDALSKGEVPVYVELDTATEQTRAGHLIFMDNVIDTASGTIRLKA
                     EFPNADNHLWPGMFVTVTLSPRTLRGALTVPVQAVQTGPENKFVYVVAADNTVSLLPV
                     DVRLIQSEIAVVKGDGLKPGMRVIVEGAQNLRPGSIVKEAGAGERAEPSALNAGPSGR
                     RAFGGQRAQRSKMV"
BASE COUNT          757 a          946 c          934 g          681 t
ORIGIN      
        1 acacccagca atatatagat caccagcaga gcggcaacaa tcaggatgac ctggctggcc
       61 tgagaagact ggaacgcagc agcatccccc gtatagctgg tcaggatact cgcaggcata
      121 ttcaactcct gcttgaattg ctcaatgcgg gcggaggcgt cgcccagcga cgcgcctggc
      181 gcgagattga atgaaacggt caacgcttca agctggccga catgattgat tgcgatgggc
      241 cctacttcac gttcgacaga tgcgacgctg gatagcggga ccagcatgtc atccttgccc
      301 cgcagataaa tctttccaaa ggcgctctcg tcggcccgcg cttcgggagt tacctggagg
      361 atgacctgat aactgtcagt ggaagtatag attgtggaaa cttgccgctc gccaaacgcg
      421 ctatacagcg tcgaccggat atcagccatc gggacgccaa gcagatcgac cttgtcgcgg
      481 ttgatcttga gccgcacctg caatcccttc agctgggaat cgttggtgac gtctcgaaac
      541 accttgtctt cccgcatctt tgccatgagg ccttcggcac ccacccgaag ctctgcaggc
      601 tgcacgctgc gcatgacata ctggtagcgg ctcttgctcg tccgcccccc cagcctgagg
      661 ttctgtatcg gagtgatgaa cacattgaca cccggaatga cgcgtacgtc cctgcgcagg
      721 ctctccatga ccttgtcaag atgagggcgt tcactgcgcg gcttgagcgt catgaacaat
      781 cttgcgctat tggtatcgct ggcgttgaca atcaacgtgt cgaccgcccg attggcccgg
      841 atcacgctgc cagttcgctg aagcaattcc gccatcgcgg ggaaggaaat atcttcgacg
      901 gcctccactg taacaagcgc ctgcccgata tcctccgtcg ggaaaaaacc tttgggcaaa
      961 acggtgaata aaacgcctgt aagaacaaat gtagctgccg ctattcccag taccacgcgc
     1021 cggttgcgca acgaccagtc gagcatgcgc tcgtagaatc gcgatgtcct ctcgaaaccg
     1081 cgctcaaacc attcggtcca tctcaggctc aggctttcgc cctgatgccc cgtcataagg
     1141 cggctggtca taacaggcac gagggtgagc gatacgacgg cggacatcag gacggctata
     1201 ccgacgactg cggcaaactc gtggaaaagc agcccgatta cccctggcat gaagaagatg
     1261 ggaatgaaaa cggcgacgag cgagagggaa atcgaaatga tcgtaaaact cacctctctt
     1321 gatccaagaa gagcagcttg caagggagac aaaccccttt caatgtgacg gacgatgttt
     1381 tcgagcatga cgatggcgtc atcgactacc agtcctaccg caagcgtaat ggcgagcaac
     1441 gagatattgt ccaggctgta atcgaggaca cgcatgaccg cgacggtgcc cagcagtgaa
     1501 atcggcaggg tcagcgccgg gatcagggtg gcataggctt tgcgcagaaa gagaaaaatg
     1561 ataagcagaa cgaggaggat ggtgatcccc agcgtcacct tcacgtcatg gatggaatgc
     1621 ctgatagact ccgaacggtc gctggtcagt ttgagttgga cggaggaagg catttgtgcc
     1681 gtgagcgtgg gaatagttgc cttgatggca tctacagtgg caaccgtatt ggctcctggc
     1741 tgacgctgca cagagagtgt gatggcccgt tccccgttga cccagcttgc ggtcttcacc
     1801 gactccacgc tgtcttccac atctgcaacc tcagaaagcc gaacgggact gccattctct
     1861 gatgttgcga caataagttt agcaaactga gcagcattgc tgagctggcg attcgcctgg
     1921 atcgtgagcg tctggcgggg accctcaagg gtcccgatag gcgtgttggc gtttgccgtg
     1981 cgaatggcag ctgcgacatc atcaagggtc agaccgcggg cggcgagcgc ttcaggattc
     2041 gcgcgcaccc tcacggcata tttttttcgc ccgttgattt gcaccagggc cacgcctgtg
     2101 agcgtcgaga gcgtggggga aataagatct tcggcatagc tgttcagctc cgacagcgac
     2161 atcgagggtg aagtcaaggc aagaaaaatg atgggagcat ccgccggatt aaccttgcga
     2221 taggaaggcg gagaggtcat ctccacaggt agcgcgcgtt gggcacgcaa taacgctgct
     2281 tgcacgtcca ccgctgccgc gtcgatatcg cggtcctgct cgaactctag ggtgatcgat
     2341 gtgttgccca gtgtgctgct cgagctgatc agcgagaggc cggaaatact ggaaaactgc
     2401 cgctccagcg gcgttgccac cgacgaagac atgatttccg gactcgcccc aggcagtgcc
     2461 gctgtcactg aaatcgtggg caagtcatag ctgggaaggg ccgcaattgg caaaccaccg
     2521 taggctatga ctcctgccag cacggcggaa gcggacagca gcatcgtcat gaccggacgg
     2581 cgaatgcaca actcggagag attcatcact gaaagaccgt cataccattt tcgaccgctg
     2641 tgctctctgg cctccaaatg ctcttctacc tgatggcccg gcgttcaaag ccgagggttc
     2701 cgccctttca cccgccccgg cttcctttac gatacttccc ggcctgaggt tttgcgcgcc
     2761 ctctacaatc actcgcatgc ccggcttaag gccatccccc ttcacgactg caatctcgct
     2821 ttggatcagg cgcacgtcaa ccggcagcaa actgactgta ttatcggcag cgacgacata
     2881 aacaaatttg ttttccggcc cggtctgaac tgcctgtacc ggcaccgtga gcgctccgcg
     2941 cagtgtccgc ggcgaaagcg tcaccgtgac gaacataccc ggccatagat gattatctgc
     3001 attcggaaat tccgccttga ggcgaatggt gccgctcgca gtatcaataa cgttatccat
     3061 gaatatcagg tggcctgctc tcgtttgctc ggtggccgta tccagttcca cgtagacagg
     3121 cacttcaccc ttggacaatg cgtcctgtaa aggcacgaac tcacgttcag ataaggtaaa
     3181 actgacattg atcggatcga tctgagtgat gtttacgagc acatcattgg gttgcacgag
     3241 gctaccctgg tatacggaaa cagccccggt gcggccggcg atgggtgccg taatctcgct
     3301 gaaaccccgg gcgattcg
//
LOCUS       DASSHJ010000023        11846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1622028,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000023 DASSHJ010000000
VERSION     DASSHJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622028"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VFI43_01190"
     CDS             complement(<1..1484)
                     /locus_tag="VFI43_01190"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7060774.1"
                     /translation="MRVSHFFLSTLKEAPAEAELVSHRLMLRAGLIKRLGSGLYTWMP
                     LGLRVLRKVENIVREEMDASGALELLMPAVQPAELWEETGRWEAFGPQMLKIKDRHQR
                     DFCFGPTHEEVITDIARREIKSYRQLPLTFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHTDVASLKEAYGKMHDAYCRIFTRLGLGFRVVTADPGAIGGSGSQEFHVLADSGE
                     DAIAFCPDSDYAANIEMAEARSAGIAREAPDGPMNKVYTPGRKTCEEVSAFLGLRIEQ
                     TVKTLAVMADGKMYLLLLRGDHSLNETKVNKVPFLAGFRFANEEEIRANTGCLPGYIG
                     PVGLPLPVIADRTVAMMSNFVCGANEVDYHFTDVNFGRDLKEPDGVFDLRDVVAGDPS
                     PDGRGMLEICRGIEVGHIFELHTKYSRAMNAVYLDESGQARPMEMGCYGIGVSRIVGA
                     AIEQNHDARGIVFPAAMAPFQVAIIPIGLKKSTRVRDEAEKLYAA"
     gene            1669..2256
                     /locus_tag="VFI43_01195"
     CDS             1669..2256
                     /locus_tag="VFI43_01195"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HET7060775.1"
                     /translation="MIDRNGYRSNVGIILLNSKNEVFWGKRIKQDSWQFPQGGIKSGE
                     SPEQAMYRELTEEIGLHPRHVQIIGRTRDWLRYDVPDQWIRRDWRGNYRGQKQIWYLL
                     RLVGRDCDVSLRASVRPEFDAWRWNQYWVELESVVEFKRQVYRQALTELARLISRPSL
                     KLHGKAYERPMGRDREQPMTELQPLKNLSPTDKPI"
     gene            2540..3535
                     /locus_tag="VFI43_01200"
     CDS             2540..3535
                     /locus_tag="VFI43_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET7060776.1"
                     /translation="MILRTIIVSLLAIGTIAAPPAMSADEPIQPIEAHKPKNPKMVEL
                     GEMLFFDPRLSKSGFISCNSCHNLSMGGSDNLPTSIGHKWHQGPISAPTVLNSSLNFA
                     QFWDGRAKDLKDQAGGPIANPGEMGFSHALAGGVLQSIPQYRARFKELFGGDKIDIGM
                     ITEAIAAFEETLVTPDSRFDKWLKGDKNAITQTELEGYKLFKEKGCVSCHNGPNVGGG
                     SYQKMGVHEPHKTTSKAEGRFGVTGKESDRFLFKVPTLRNVELTYPYFHDGSAATLEE
                     AVNTMGRLQLGHKFTKDENAKIVAFLKTLTGKQPHIVLPILPPSTNETPRPKPFD"
     gene            3873..4118
                     /gene="hfq"
                     /locus_tag="VFI43_01205"
     CDS             3873..4118
                     /gene="hfq"
                     /locus_tag="VFI43_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET7060777.1"
                     /translation="MSAKGQLLQDPFLNTLRKEHIPVSIYLVNGIKLQGHIDSFDQYV
                     VLLKNSVTQMVYKHAISTVVPARAVNIPFEAPGTPDA"
     gene            4111..5295
                     /gene="hflX"
                     /locus_tag="VFI43_01210"
     CDS             4111..5295
                     /gene="hflX"
                     /locus_tag="VFI43_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7060778.1"
                     /translation="MLDRSAGSTPGAKIAADTAILVSIDFTDKTNDESLEELRQLAAS
                     DHLRISSVVKGKRSSPDPSTFAGAGKVNEIASIAEQTGASLVIFNHDLSPAQQRNLEQ
                     RLERRVIDRTSLILDIFAQRAHSHEGKLQVELAQLEHLSTRLVRGWTHLERQKGGIGL
                     RGPGETQLETDRRLLGKRVKLLKEKLKKVQSQRELQRRSRRRADVVSVSIVGYTNAGK
                     STLFNRLARAHTYVADQLFATLDPTTRKVFLPDHGPLVISDTVGFIRDLPHTLVAAFR
                     ATLEETVQADLLLHVVDASSPNRDEQIDEVNKVLKEIGADSLPQVLVLNKIDLTDLSP
                     EAAGYRRDECDRITQVRLSAKTGQGLELIKLALSEAIEQHASQHCKNQSRITDAVVAS
                     RA"
     gene            5392..6582
                     /gene="hflK"
                     /locus_tag="VFI43_01215"
     CDS             5392..6582
                     /gene="hflK"
                     /locus_tag="VFI43_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET7060779.1"
                     /translation="MGLNDPQWGRKKNSSGPPDLDQLWRNFNKKLNSLFKRKGNGGSG
                     GGEGQGPATPRQYGGGIGLLIALLAILWIASGFYIVNEGQRGVVLRFGKYVESTQAGL
                     RWHLPYPIEVVETVNVSQVRTVEIGYRNNVRSKVLKESLMLTDDENIIDIQFAVQYIL
                     KNPEDFLFNNREPENAVLQAAESAIREIIGKSKMDFVLYEGREQVAAKATQLMQEILD
                     RYKIGIAISKVTMQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDVIPKAKGNA
                     ARLLEESEGYKQRVIASSEGEASRFKQILVEYNKAPAVTRDRLYLDAMQQVLSSTSTI
                     FVDQKSGSNLLYLPLDKLIQMSGPTSPSSQASEVPVAPAQESAPDSAARTREAFRGRE
                     RETR"
     gene            6582..7460
                     /gene="hflC"
                     /locus_tag="VFI43_01220"
     CDS             6582..7460
                     /gene="hflC"
                     /locus_tag="VFI43_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET7060780.1"
                     /translation="MKKYASILLGTLIALIVLATASLYVVDERQQAILFQLGEIVDVK
                     TSPGLYVKIPLAQNVRYFDARILTLDTAEPERFITSEKKNVLVDLFVKWRIVDVKQYY
                     VSVRGDEMLAQTRLAQTVNSSLRDEFGNRTVHDVVSGERDKIMEIMRQKADADARKIG
                     VEVVDVRLKRVDLPQEVSESVFRRMEAERKRVANELRSTGAAESEKIRADADRQREVI
                     MAEAYRKAQEVKGEGDAKASAIYGAAFQPNPEFYSFYRSLEAYKQSFKNKNDLMVIDP
                     SSEFFKYLKSPNRSAK"
     gene            7620..7805
                     /locus_tag="VFI43_01225"
     CDS             7620..7805
                     /locus_tag="VFI43_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HET7060781.1"
                     /translation="MWDILGMAFALMLVLEGLLPFLMPGAWRDTFRKLTEISDNQIRF
                     VGLTSMLAGLLILYLVT"
     gene            7833..9002
                     /locus_tag="VFI43_01230"
     CDS             7833..9002
                     /locus_tag="VFI43_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET7060782.1"
                     /translation="MRQWILPEYVEDILPGEALKIEMMRRRIIDLLMSHGYELVGPPL
                     LEFVESLLSGSGGETNLRMFKVVDQLSGRMMGLRADMTPQVARIDAHLLNRKGITRLC
                     YAGSVLHTLPSGLTRTREPVQIGAELFGHSRLESDLEVQKLMLDSLAAGGIGKVHLDL
                     GHVAVFRGLIRGSGIPDELESELYRALQSKDFETLENIDTGFDRQVDRRARQALLLLP
                     QLYGGFEVLDRARSSLPDYPDIRQALDELETVGKELKPLVDTLAFDLADLRGYHYHSG
                     MVFAAYASNCPNAVALGGRYDEVGKAFGRARPATGFSMDLRELAGLVQTGPEKKGILA
                     PYPAGNERLGEKIEQLRQAGETVVIALPGSEHEQNVLTCDRKLIQQNGIWTLVTV"
     gene            9074..10381
                     /locus_tag="VFI43_01235"
     CDS             9074..10381
                     /locus_tag="VFI43_01235"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7060783.1"
                     /translation="MTKNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL
                     VIKGKQTVLHLIPSGILREEVACYIGNGVVISPQALLDELGMLEQAGVDVQNRLRISE
                     ACPLILPCHTALDSARETARGIDKIGTTGRGIGPAYEDKVARRAIRLQDLFHRDRFAA
                     KLGEMLDYHNFVLKNYFHAPSVDFQQIMDDTLSLADRIKPMVADVPRLLFEANRTGSN
                     LLFEGAQGTLLDIDHGTYPFVTSSNCIAGAATTGSGVGPQMLHYVLGITKAYTTRVGG
                     GPFPTELDDDVGKHLAKRGNEFGATTGRPRRCGWFDAAALKRSVQINGVSGLCVTKLD
                     VLDGVETLRLGVGYRLADDSARQSFHEILPVGADELARCEPVYEEMPGWKDSTVGVEK
                     FAQLPQAAQNYLKRIEEVCEVPVDMISTGPDREETIVRRHPFE"
     gene            10408..10890
                     /locus_tag="VFI43_01240"
     CDS             10408..10890
                     /locus_tag="VFI43_01240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7060784.1"
                     /translation="MRTSPGSSQNSTNACSVRLIDWQRDSRALRDIREAVFIREQNVP
                     AELEWDEFDVQCLHFLAFNPEGNPVGTARLLLNGTAGTLGRMAVLKEWRVRGVGSALV
                     HGLLGEAKKRGISELALHAQTHAADFYERFGFRIEGKQFMEAGIPHVKMILHLQASVC
                     "
     gene            complement(10957..>11846)
                     /locus_tag="VFI43_01245"
     CDS             complement(10957..>11846)
                     /locus_tag="VFI43_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7060785.1"
                     /translation="QVYAEAYVPVGNGLNVKVGHFYTPIGYESVPAPDNFFYTHAYTM
                     QYGEPFTHTGVIGNYKITNNWSVIGAAVTGSATGGWDGAFDKQLGNWGGIAGITWTSD
                     DMGSSANLSGTYGETSTRSSEPWGMYSLVLKHRFTEKTHFVFQHDHGYAGGVLLNGVN
                     QNAQWFGINMHLYYDLTPDLSVGVRTEWFRDRDGFRVFAPGRVAAATDNQGFSYALGR
                     NQLGNSTSVPADYYAITAGMNWKAARTLKLDWKPIRQFNVRPNVRYDNVDALHGAIYR
                     PFGGHKDQVVLSLDFMLPF"
BASE COUNT         2838 a         3132 c         3283 g         2593 t
ORIGIN      
        1 gcggcgtaca gcttttccgc ttcatccctt acccgcgtac ttttcttcag cccaatggga
       61 ataatggcaa cctggaaggg cgccatggct gcagggaaaa caattccgcg ggcatcgtgg
      121 ttctgttcaa tggcagctcc cacgattcgc gacacgccaa tcccgtagca tcccatttcc
      181 atgggccggg cctggccgga ctcatccaga taaacagcgt tcatcgccct ggaatatttc
      241 gtgtgcaact caaaaatgtg ccccacctct atgccacggc atatctcgag cattcctctt
      301 ccatccggcg aaggatcgcc ggccaccaca tcacggagat cgaacactcc gtcgggttcc
      361 ttcaggtcac gcccgaaatt aacgtcggtg aagtggtagt cgacttcatt cgccccacac
      421 acgaaattgc tcatcatggc gaccgtgcgg tcagcgatta ccggtagcgg caggccgaca
      481 gggccaatat atccgggaag gcaacctgtg tttgcgcgaa tttcctcctc gttcgcgaat
      541 cgaaacccgg cgaggaaggg aaccttgttg acctttgtct cattgagcga atgatcgccc
      601 cgcaacagca acagatacat ttttccatcc gccatcaccg ccagcgtttt cacagtttgt
      661 tcgatgcgaa ggccaagaaa cgcagagacc tcttcgcacg ttttcctgcc gggtgtataa
      721 accttgttca tcgggccatc gggcgcttcc cgcgcgatcc ctgccgaccg cgcctcggcc
      781 atttcgatgt ttgccgcata atctgaatca gggcaaaagg cgatggcatc ctcgcccgaa
      841 tctgccagga catgaaactc ttgtgagccg ctgcctccga ttgctccggg atctgcggtc
      901 acgacacgga accccaatcc caggcgggta aaaatgcggc aatatgcgtc gtgcatttta
      961 ccgtacgcct ctttcaggct cgcaacatcg gtatggaacg aatacgcgtc cttcatgatg
     1021 aactcgcgcg cccgcatgac cccgaaccgc ggccggatct catcgcgaaa cttcgtctgt
     1081 atctggtaga aggtgagcgg caattgacgg tagctcttga tttccctccg ggcgatgtcg
     1141 gtgatgactt cctcatgagt aggcccaaag cagaaatcac gctggtgcct gtccttgatt
     1201 ttcaacatct gcgggccaaa cgcctcccag cggccggttt cctcccataa ttccgccggc
     1261 tgtaccgcag gcatcagcaa ttcgagcgct ccactcgcgt ccatttcttc gcgcacgata
     1321 ttttcgacct tccgaagcac cctcaacccc aaaggcatcc atgtataaag gccgctgccg
     1381 agacgcttga tgaggcccgc gcgcagcatg aggcgatggc tcaccagttc tgcctccgct
     1441 ggagcctctt tcagggtgga aagaaaaaaa tgtgatacgc gcatgagtgt accgtgcctt
     1501 ggaaaaatcc ttgattctac cgtgaaaagg ggatgagaac gccagatgga agacgacaaa
     1561 tatcggcagc ccaagattgg ccgctggctt cgatatacag tttcgactgc ttcaaatgtg
     1621 ggataaaatg tggaaaaatt tccctattcg ggttaatggg tgaatcgcat gatcgatcgt
     1681 aacggatatc gctccaacgt tggcatcatc ctgctgaatt ccaagaacga ggtgttttgg
     1741 ggcaagcgaa tcaaacagga ctcctggcag tttccccagg gaggcatcaa atccggtgaa
     1801 agccccgagc aggcaatgta ccgggaattg acggaagaga tcgggctgca tccccgtcat
     1861 gttcaaatca ttggccgtac ccgggactgg ctccgctatg atgttcccga tcagtggata
     1921 aggcgtgact ggcgaggaaa ctacaggggc cagaaacaaa tctggtacct gctgcgcctg
     1981 gtggggcgtg actgcgatgt gtcgctgcgt gcaagtgttc gaccggagtt tgacgcctgg
     2041 cgctggaatc aatattgggt agagctggaa tcggtggttg aattcaaacg gcaagtgtat
     2101 cgacaggcgc tgactgagct tgcccggctg ataagccgac catcgctcaa gttgcacggc
     2161 aaggcgtatg aaaggccgat ggggcgtgac agggaacagc cgatgaccga actgcaaccg
     2221 ctaaaaaatc tctctcccac agacaagccc atatgaatcc ctttataaat ccctttcccg
     2281 aggccagacc tgttctggca gctttcatga aataccccta tctgtatcgg cccttcttgt
     2341 ctgttttatg cgctgtccgc cttccctcca actgtgagag atagccgggg catgggcaaa
     2401 ggtttgatta ccattcaagg gcaatgtgat tttcaaatgt cctggatttt atgtcagata
     2461 aaaacaagag gttcaataac ggataatttg taggcgtatc ggctgcgcca ccacagccgt
     2521 gtttttaggg agaccaacga tgatattgcg aacaataatt gtatcgctgc tggcgattgg
     2581 aaccattgct gccccaccgg caatgtctgc tgatgaacct atccagccca ttgaagcgca
     2641 caagccgaaa aatccaaaaa tggtcgagtt gggtgaaatg ctgtttttcg acccccgcct
     2701 ttccaaatcc ggattcattt cctgcaattc ttgccataac ctcagcatgg gtggatcgga
     2761 taatttgcca acctccattg ggcacaaatg gcaccaaggc ccgatcagcg ctccaaccgt
     2821 tctgaactcc agcctgaact tcgcccaatt ctgggatggc agagccaagg acctgaaaga
     2881 ccaggcgggt ggcccgattg caaatccggg agagatgggg ttcagccacg ctttagccgg
     2941 gggtgtatta cagtcgattc cccaataccg cgcacgtttc aaggagctgt ttggcggcga
     3001 caaaattgat atcggtatga tcacggaggc aattgctgcc ttcgaggaaa cactggtgac
     3061 gccggattcg cgtttcgaca agtggctcaa gggcgacaag aatgcaatca cccagacgga
     3121 actggaaggc tataagctct tcaaggagaa gggttgcgtg agctgtcaca atggccctaa
     3181 cgtgggtggc ggctcctatc agaagatggg cgttcatgag ccgcacaaaa cgacaagcaa
     3241 ggcggaaggc cggtttggcg tcactggcaa ggaatccgac cgcttcctgt tcaaggttcc
     3301 caccttgcgc aacgtggaac tcacctatcc gtattttcat gatggctccg cggcaaccct
     3361 cgaagaagcg gttaatacca tgggccgcct ccagctgggc cacaagttca cgaaggatga
     3421 aaacgcaaaa atcgtcgctt tcctgaagac gctgaccgga aagcaaccgc acattgtgct
     3481 gcccatcctg ccgccctcaa ccaacgagac gccaaggccg aagccgtttg attgacacac
     3541 ggctgaagct tatcccgcga gacgtttatt tatctgccgc ctttttttga cccacttatt
     3601 gcccgggcgt caggcccggg caatattcca tcatactaaa ccatactgct ccccctctcg
     3661 cgattcaaag ccttctgaac ttccctcgag ttcctcgctc atatcaaacc ggagagtctg
     3721 cgcaaagccg catgacgagc cgaatttcgc catctcgaac cattactttt ggcccagtgt
     3781 cgcctgtatt tcatcttccg tccaagaata tggatttact cctgaaaatc aagtagagtt
     3841 atttcttgaa taactaaaat acggagtctc ccatgagcgc taagggccaa ttgctacaag
     3901 acccgttcct gaatacgttg cgaaaggaac atattccagt atcaatttat ctggtaaacg
     3961 ggatcaagtt gcaaggacat atcgactcgt ttgatcagta tgtggtgttg ctgaagaata
     4021 gcgttaccca gatggtatac aagcacgcaa tttccaccgt cgttcccgcg agagcggtta
     4081 atattccatt cgaggcgccc ggcacacctg atgcttgatc gctccgcggg aagtacaccc
     4141 ggcgcaaaga tcgcagctga cactgcaata ttggtcagca tcgattttac cgataaaaca
     4201 aatgatgaaa gcctcgaaga gctgaggcaa ctggctgcca gcgaccatct ccggatttcg
     4261 agcgtggtca agggcaagcg ctcaagcccc gatccgtcga ccttcgcagg agccggcaag
     4321 gttaatgaga tcgcatccat tgcagagcag accggcgcat ccctggttat cttcaatcac
     4381 gatctgtctc ctgcccagca acgcaacctg gagcaacgcc tcgaacggcg agtaatagat
     4441 cgcacaagcc tgatcctgga catctttgcg caacgcgccc acagccacga aggcaagttg
     4501 caggtggaac tcgctcagct tgagcatcta tcgacgagac tcgtgcgcgg ctggacgcac
     4561 ctggagcggc agaaaggagg catcggtttg cgcggccctg gtgagaccca gttggagacc
     4621 gaccggcgcc tgctgggaaa gcgggtgaaa ctccttaaag aaaaactcaa aaaggttcag
     4681 tcccagcgag aattgcaacg ccgttcccgc cgtcgcgcgg atgtcgtgtc ggtatccatt
     4741 gtcggttata cgaatgccgg caaatccacc ttgttcaatc gcctcgcccg cgctcacact
     4801 tatgttgcgg atcaattgtt cgccaccctc gacccgacga cccgtaaggt atttttgcct
     4861 gatcacggcc cccttgtcat ttccgatacc gtcggattta ttcgcgacct gccacatacg
     4921 ctcgtcgcgg cttttcgcgc cacactcgag gagactgtgc aagctgatct cctgcttcat
     4981 gtcgtcgatg cgagcagtcc gaaccgggat gagcagatcg atgaagtgaa caaggtctta
     5041 aaggaaattg gggccgactc ccttccccag gtcctggtgc tgaacaaaat cgatttgacc
     5101 gatttatcgc ctgaggcggc cggttatcgt cgtgatgagt gtgatagaat tacgcaggtc
     5161 cgattaagcg ccaagaccgg ccaggggctg gaattgatca agcttgcgct ctcggaagcc
     5221 atcgagcaac atgcctcgca gcattgcaaa aaccaatccc ggattacaga tgcggtggtg
     5281 gctagccgcg cttagcttga aatcaaggcg aatttttgtg ctgggaaact tgaatttcgt
     5341 ttccattcgc tttggtcgtg gggccgcatt aaacaaaaga caggaatcat catgggatta
     5401 aacgatcccc agtgggggag aaaaaaaaat agttcgggtc cacctgatct ggatcagcta
     5461 tggcgtaatt tcaacaagaa actcaatagc cttttcaagc ggaaggggaa tggaggttcc
     5521 ggcggaggag aaggacaggg tccagcgacg cccaggcaat atgggggcgg gatcggcctg
     5581 ctcatcgcat tgctcgcgat cctgtggatt gcaagcggtt tctatatcgt gaacgaaggg
     5641 caacgaggcg ttgtattgcg ttttggaaaa tacgttgaaa gcacccaggc gggtctgcgc
     5701 tggcatcttc catacccgat cgaggtcgtt gaaacggtga acgtgagcca ggtgcgtacc
     5761 gttgaaatag gttaccggaa taacgtgaga agcaaggttc tcaaggaatc gctgatgctg
     5821 acggatgatg aaaatatcat cgatatacag tttgcagtgc aatatattct gaagaacccc
     5881 gaggactttc ttttcaacaa tcgcgagcct gaaaacgccg tgctgcaggc agccgagagc
     5941 gcgataaggg agatcatcgg caaaagcaag atggattttg tcctgtacga aggacgtgaa
     6001 caggttgccg cgaaggcaac tcaactgatg caggagatcc tggatcgcta taagataggt
     6061 attgcgatca gtaaggtgac gatgcaaaac gctcagccgc cggagcaggt gcaagccgcg
     6121 ttcgacgatg cagtcaaggc tggacaggat cgggagcggc agaaaaacga gggccaggca
     6181 tacgccaacg acgtgatacc caaggccaag ggtaacgccg cacggcttct ggaagagtcg
     6241 gagggataca aacaacgcgt cattgcaagc tccgagggtg aggcgagccg tttcaagcag
     6301 attctcgtgg aatacaacaa agcgccggct gtaacgcgcg atcgtcttta cctggatgcg
     6361 atgcagcagg tgctctccag tacgagcacc attttcgtag accagaaaag cggcagcaac
     6421 ctgctgtatt tgccactcga taagctgata cagatgagcg gtcctacctc gccatcttcc
     6481 caggcgtcgg aggttccggt cgcgccggct caggaatcag cgccggacag tgccgcacgg
     6541 acccgcgaag catttcgtgg acgcgagagg gagacccgat agtgaaaaaa tacgcttcca
     6601 tactgctggg tacgcttatc gccctgatcg ttctggcgac cgcttcactt tacgtcgtcg
     6661 acgagcgcca acaggcgatc cttttccagc tcggcgagat cgttgacgtt aaaacatctc
     6721 ccggcctgta tgtcaagatt ccacttgcgc agaacgtccg gtattttgat gcccgaatcc
     6781 tgacgcttga taccgccgag cctgaacgct tcattacttc tgaaaagaag aatgtgctgg
     6841 tggatctgtt tgtgaaatgg cgcatcgtcg acgtcaaaca gtattacgtc agtgtgcgag
     6901 gggacgaaat gctcgctcaa acacgcctcg cgcaaacagt caattcgagc ctgagggacg
     6961 aattcggcaa tcgtaccgtc catgatgtcg tttcgggaga acgcgacaag atcatggaaa
     7021 tcatgcgtca aaaagctgat gccgacgctc gcaagatcgg ggtggaagtc gttgatgttc
     7081 gtctgaagcg cgtggacttg ccgcaggagg taagcgaatc cgtcttccga aggatggaag
     7141 cggagcggaa acgcgtggcc aacgaacttc ggtcaaccgg cgcggctgaa tccgagaaaa
     7201 tacgggccga tgcggatcgc cagcgcgagg tcattatggc ggaggcttat cggaaagctc
     7261 aggaagtgaa gggtgaaggt gatgcgaagg cttcagcgat ctatggcgca gcgtttcaac
     7321 ccaacccgga gttctattcg ttctatcgga gcctggaggc ctataagcag agcttcaaaa
     7381 ataaaaatga cctgatggtg atcgatccca gttccgaatt tttcaaatac ctgaaaagcc
     7441 ccaaccggtc tgcaaagtag ccataggcgg gcggagccat agcgcgtgcc cgccatgccc
     7501 atcccctagc ccgaactaaa aaacaataaa tgaaatgctg ttgcctcgaa ttcccgcgat
     7561 ttcctcccga tgtgcttgca tgaagcagca aatttgcagg gcttaataag gactgcggaa
     7621 tgtgggatat tctgggaatg gcctttgcac tcatgctcgt gctcgaaggt ctccttccgt
     7681 ttcttatgcc cggagcatgg cgcgacacct ttcgaaagct caccgagatc agcgataacc
     7741 agatccgttt tgttggctta acctccatgc tcgcggggtt gttgatactt taccttgtca
     7801 cgtaaattct ccagacgtaa tcaggcgtaa ccatgcgtca gtggattctt ccagaatatg
     7861 tagaggatat cctccctggg gaggcgctga agatcgagat gatgcgccgc agaatcatcg
     7921 atctcctgat gtcccatggc tatgagctgg tgggaccgcc cttgctcgag ttcgtcgaat
     7981 ccctcctttc agggagcggg ggtgagacta atttgaggat gttcaaggtc gtggatcaac
     8041 tcagcggccg gatgatggga ctacgtgctg acatgacacc acaagttgcg cgaattgacg
     8101 cgcatttgct taatcggaaa gggattaccc gcctctgcta cgcggggagc gtattgcata
     8161 ccttgccatc cgggctgaca cgaacccggg agccggttca aatcggagcc gagcttttcg
     8221 gtcactcccg tctggagagt gaccttgaag ttcagaagct tatgctcgat tcgctcgcgg
     8281 ccggcgggat aggaaaagtc catctcgacc tcggccacgt cgctgttttc cgcggtttga
     8341 tcagaggctc agggataccc gatgagcttg aatccgagct gtacagggcg ctccagtcga
     8401 aggacttcga aacgcttgag aatatcgata ccgggttcga caggcaggtg gatagacgcg
     8461 cgaggcaggc attgttgctt ttgcctcagt tatacggcgg attcgaggtt ctcgatcgag
     8521 cccgctcgtc gcttccagac tatccggata tcagacaggc actggatgaa ttggaaacgg
     8581 tcggcaagga gctgaagccg ttggtcgaca cgctggcgtt tgacttggcg gacctgcgag
     8641 gttaccacta ccacagcgga atggtatttg cagcttacgc aagcaattgc cccaatgctg
     8701 tagctcttgg cggacgttat gacgaggtgg ggaaggcatt cgggagagca cgtccggcaa
     8761 ccggattcag tatggatctg cgggaacttg ccggtcttgt ccaaaccggg ccagagaaaa
     8821 agggaattct tgcgccctat ccagccggta atgagagact cggagaaaaa atcgagcagc
     8881 tgcggcaggc gggtgaaaca gtggtgatcg cattgccggg atcagagcat gaacagaatg
     8941 ttttaacctg cgaccggaag ttgatacagc aaaatgggat ttggacgctt gtgacggtct
     9001 agcaggcaga ccggtagtct gctggagtcc aggcagcggc ctgccaggga aaacgctttc
     9061 ttaagacagg aatatgacaa aaaacgtggt cgtcatcggt actcaatggg gcgatgaagg
     9121 caagggaaaa atagtagatt ggctaacgga tcacgcgcag ggcgtggttc gcttccaggg
     9181 ggggcataac gctgggcaca cgctcgtcat caaaggaaag cagaccgtac tccacctgat
     9241 cccttccggt atcctgcgtg aggaagtggc ctgctatatc ggcaatggcg tggtgatatc
     9301 ccctcaggca ctattggatg agctggggat gctggaacaa gcgggggttg acgttcagaa
     9361 tcggcttcgc atcagcgaag cttgtccatt gatattgccg tgccacactg cgctggatag
     9421 tgcgcgcgaa acggccagag gaatcgacaa gattggcacg acggggcgag gcatcgggcc
     9481 cgcctatgag gataaagttg cgcggcgggc catccgcctg caggatctgt ttcatcgaga
     9541 ccggtttgca gcaaagcttg gcgaaatgct tgactaccat aatttcgtcc tcaagaatta
     9601 ttttcatgcg cccagcgtcg atttccagca gatcatggat gatacgctct cgcttgcaga
     9661 ccgcatcaag ccgatggtcg ctgacgtacc caggttgctg ttcgaagcga acaggacagg
     9721 aagcaatttg ctatttgaag gcgcgcaagg aacgctgctt gatatcgatc acggcaccta
     9781 ccctttcgtg acctcgagca attgcattgc aggcgcggca acaacgggaa gcggcgtagg
     9841 cccccagatg cttcattatg tgctaggcat tacgaaggcg tataccacga gggtgggtgg
     9901 cgggcctttt cccacggagc tggatgatga tgttggcaag caccttgcga agcggggcaa
     9961 tgaattcggg gccactaccg ggcgcccacg gcggtgcggg tggtttgacg ctgcggcgct
    10021 gaagcgatcc gtccagataa acggggtgtc ggggttatgt gttacaaaac tcgacgtact
    10081 ggatggggtt gaaaccctgc gtttgggcgt ggggtacagg ttagcggatg acagcgctag
    10141 gcagagcttt catgaaattc tcccggtagg cgcggatgaa cttgcacggt gcgagcctgt
    10201 gtatgaagaa atgcccggct ggaaagacag tacggtgggg gtggagaagt ttgcccaact
    10261 cccgcaagcg gcgcaaaact acctgaagcg tattgaggaa gtctgcgaag tgccggtgga
    10321 catgatatca accggaccgg atcgtgagga aaccatcgtg cggcgacacc cttttgagta
    10381 agcgttaagt aagggtcaag caagggattg agaacttcgc ccggaagcag tcagaattca
    10441 acgaatgcgt gtagcgttcg cctgatcgac tggcagcgcg actcccgtgc cttgcgagac
    10501 atacgtgagg cggtcttcat tcgtgagcag aatgttcctg ccgaactgga gtgggatgag
    10561 tttgacgtac aatgcctcca ctttctcgct tttaaccccg aaggcaaccc tgtcgggacc
    10621 gcccgcctgt tgctaaatgg tacagctggt accctcgggc gaatggcggt gctgaaggaa
    10681 tggcgagtca ggggtgtggg cagcgcactg gttcatggtt tgctggggga agcgaaaaaa
    10741 cgtggaattt cagaactcgc cctgcacgct cagacccatg ccgccgactt ttatgaaaga
    10801 tttggtttca ggattgaagg caagcagttc atggaagccg gaattccgca tgtaaaaatg
    10861 atcctgcatc tgcaagcttc agtttgctga aacacgcaag cggaatgaaa ggcagatcgg
    10921 gctcttgatc ctatggatac tgccctatga gcaatatcag aacggcaaca tgaaatcgag
    10981 ggatagaacg acctgatcct tgtgccctcc aaagggccgg tagatcgccc cgtgcaacgc
    11041 gtcaacgtta tcatagcgga cgtttggacg gacattgaat tgccgtatcg gcttccagtc
    11101 gagctttaag gttctcgccg ctttccagtt catgcccgcc gtgatcgcgt aataatcagc
    11161 gggcacactg gtgctgttgc ccaactggtt tcgccccaat gcgtagctga atccctggtt
    11221 atccgtcgct gctgccacgc ggcctggggc gaagacacgg aagccgtccc tgtcgcggaa
    11281 ccactccgta cgtacgccga ccgagagatc aggggtgagg tcgtaatata aatgcatatt
    11341 aatgccgaac cattgcgcat tttggttcac tccgttcaac aacacccccc ccgcataacc
    11401 gtggtcgtgt tgaaagacaa aatgcgtctt ctcagtaaac ctgtgcttca gcaccaggct
    11461 gtacatgccc catggttcgc tgctgcgagt cgatgtttct ccataagtgc cgctgagatt
    11521 agccgagctt cccatatcgt cgctggtcca agtgataccg gcgatgccgc cccaattccc
    11581 tagctgctta tcgaacgctc catcccaacc ccccgttgcg cttccggtca ccgcagcgcc
    11641 aattacggac cagttgttgg taatcttgta attacctata accccggtat gcgtaaaagg
    11701 ctctccatat tgcatcgtgt atgcgtgggt atagaagaaa ttatcgggag caggtaccga
    11761 ctcatatcca ataggcgtat aaaagtgccc gaccttcacg ttcagtccgt tccccacagg
    11821 aacatatgcc tctgcataca cttggg
//
LOCUS       DASSHJ010000024        10353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1074202,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000024 DASSHJ010000000
VERSION     DASSHJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074202"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFI43_01250"
     CDS             <1..292
                     /locus_tag="VFI43_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7060786.1"
                     /translation="PTTHAFLGQVLDANNHPLMIDGLWAIGHGNGGAAGNTNLLYFTA
                     GPNDESHGLFGVLTPVPEPSEYTMMLAGLGAVLLLRRRRSGSDVGDKARVQV"
     gene            complement(497..655)
                     /locus_tag="VFI43_01255"
     CDS             complement(497..655)
                     /locus_tag="VFI43_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060787.1"
                     /translation="MLSIPLLILGALLMLLAPENIWIGVLLFCVGVGVELAGLVLAHR
                     DKRTGGGE"
     gene            868..4800
                     /gene="hrpA"
                     /locus_tag="VFI43_01260"
     CDS             868..4800
                     /gene="hrpA"
                     /locus_tag="VFI43_01260"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET7060788.1"
                     /translation="MKYFLNLEERLANLPHPVYSDDLPVVARREEIARAIGENQVVIV
                     CGETGSGKTTQLPKICLELKRGAAGMIGHTQPRRIAARTVAARVASELNSPVGHAVGY
                     KIRFSDKVSAQTYIKLMTDGILLAETQGDPSLQAYDTLIIDEAHERSLNIDFLLGYLK
                     QLLPRRPDLKLIVTSATINARRFSEHFNAAPVIEISGRMYPVEVRYRAFDSVHSSGGA
                     DLARDAGQPEAREKTGGKPDERDEGDIGQAILDAVDEAVREGPGDILVFLPGEREIRE
                     MAEALRKHAFGRPGRQMGGGGILGVDILPLFARQSYADQERVFKPGGSSVRRVVLATN
                     VAETSLTVPGIRYVIDTGLARLNRYSYRNKVEQLRVEKISRASASQRAGRCGRVMSGI
                     CMRLYSEEDYLSRPEFTDPEILRSSLASVILRMKALRIGAMEDFPFLDPPLPRMIADG
                     YQLLAELGAVDESNTLTKVGWRLAKFPIDPRIARMILAAKDENCLREVLVIAAALSIQ
                     DPRERPFERQEAADRAHQRFQDERSDFLAYLKLWEFYDELLKHKKSNRKLVTDCQIHF
                     LSHRRMREWREVHGQLHALVLETGLRPNEIPASYEEIHRALLAGLLGNIGFKTEEDGE
                     YLGARGIKFSIFPGSVLKKAKPKWVVAAELTETTRLYARVVAKIDPAWIEKIAGGLCK
                     KQYFDPHWEKATAQVAAFERVTLYGLTIVPKRRVYYGAVNPREAREIFIRSALVAGEY
                     ATRAPFLEHNQNLVESIAELEHKARRPDVLVDEAVIFSFYDDIIPDRIANGAAFEKWR
                     KQAERVNPCLLYLNRDDLMRHAANGVTEERFPDSVMVDGESVPLTYRFDPGHVLDGVT
                     VTVPLPLLNKLESTRFDWLVPGLIREKVTWYLKALPKQIRRHVVPVPDFVTAFLEYRE
                     SKPEVVNKTIPLTKALAKFILFKTGITVPPDIWQDEKPPPHLLVNYKVVDDAGQELAM
                     SRDLEQLRAQLGQAARLMFAGAVGQAPPERVPIERDDIKRWDFGDLPEKIAFSHGGKK
                     LTGFPALVSGEGKVAIQLFDTAEAAQASMRGGVRQLLRFELGEQMKQLEKDLSGQSRS
                     LSQAFLQLHMLIRPDALREDILNAIADRAFIGNDPLPRTEKEFAERRQRARTRLPAVT
                     DAVIRTVSDVARDVQALLTRISGTAGATRVGQPKSLVLPEAAARLKAELNEELRKLIY
                     PGFLEATPWEKLPHLPRYLKGMLLRLEKYPGNPERDQRHARAIAALWQQYEQRLEKHR
                     KSGISDATLAEFRWQIEELRVSLFAQELKTPQPVSVKRLQKLWEGMTA"
     gene            complement(4820..5803)
                     /locus_tag="VFI43_01265"
     CDS             complement(4820..5803)
                     /locus_tag="VFI43_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HET7060789.1"
                     /translation="MEYYPRWLGGIAGVGGFAKGEGSTMNVSQLNNEHGITDHIKFIE
                     GTGGFPYIRVDNAKASAIISVYGGQVLSYQPANEPHNLMFLSEKAYYQPGKSIKGGTP
                     VCWPWFGPHPEGRGRPLHGFARNRFWEVVRTEATPEEEGKVTLRLNDSEETRGLWPHS
                     FNLVLEITVGDTLNLELITRNTTSETFSITQALHTYFRVGDISQVIISGLAGAAYVDK
                     TDNGAIKFQAGPVTIDKEVDRIFRNVPAELVIDDKAFDRRIRINSKGSNSAVVWNPWV
                     KISSEMADLNDDDYEYFVCVETTNADLDVVQVPPGGEARLAANYRIECDIS"
     gene            6096..7316
                     /locus_tag="VFI43_01270"
     CDS             6096..7316
                     /locus_tag="VFI43_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET7060790.1"
                     /translation="MTGAADDDPSGIATYSQVGASFGYSMLWATFFTFPLMVGIQIIS
                     ARIGRVTGEGLAANIRKRYPSWLLYGIVCLVLIANTINIAANIGAMGAALKLLIGGPA
                     HLYAVLFALTSLVLQIFIPFPRYAPILKLLTFSLFAYVGTVFVVNVPWGEVLYKTILP
                     SISLEAEYAVAVVAIFGTTISPYLFFWQASQEVEEQQAAQGEEPLKKAPEQAEDNLKR
                     IDIDTYVGMGFSNLIAFFIMLTAAVTLNLNGVTDIETTEQAAEALRPIAGEFAFLLFS
                     GGIVGTGLLSVPVLAGSAAYAVAESFNWRIGLGRKLLEARGFYAILSVATLMGVALNF
                     TPINPIDALFWSAVINGVIAVPIMAVMMLMATRADVMGPFVVTTGLRILGWLGTGVMA
                     LAVLAMALTWNSGT"
     gene            complement(7393..7893)
                     /locus_tag="VFI43_01275"
     CDS             complement(7393..7893)
                     /locus_tag="VFI43_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HET7060791.1"
                     /translation="MKKSAETPHWGDQRFEQPGFEKQESKIPADVIDAYRSAHYQAGI
                     GRNSVTLRVDHYSESLSRLFSASDHRGAAFITACNPGGVRQRPQENRAACERLRERLN
                     QYVSGPDDIIEGMGLDPSGDWPGEESFLALGLDLEVSRTLGAEFQQNAVLWANADAIP
                     RLILLR"
     gene            complement(7890..9200)
                     /locus_tag="VFI43_01280"
     CDS             complement(7890..9200)
                     /locus_tag="VFI43_01280"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7060792.1"
                     /translation="MGQQPQSQGSQLPRIVILGAGFGGLTCAKALRNASAELTLVDQR
                     NFHLFQPLLYQVATAALSPADIAMPIRRILRYQLNAKVILGHVEAVNRQAKTVRLREN
                     ARIIPYDFLVVATGARHSYFGHDDWEAFAPGLKQIEDALDIRNRVLDALERAEQTKDT
                     EEQKRLLSFVIIGGGPTGVELAGAIAELAKIELAQASRTLKGLSAHIILIEAGPQILP
                     VFVPELAEKARQSLEHLGVEVLTGSAVEHCDAAGVIAGGRRIEARTLIWAAGVAASPA
                     AQWLDVEPDRAGRVRVQGDLSLPGDSSIFVIGDTAAVTGPDGKPVPGIAPAAKQEGKF
                     VAELLLQRINAAGDIGSNRERIHPVFHYHHAGNLATVGHKSAVLDFGWLRLSGFVAWL
                     LWGLVHIFFLIGFRNRMVVMLGWLWSYFTSDRSVRLITGSGREI"
     gene            9848..>10353
                     /locus_tag="VFI43_01285"
     CDS             9848..>10353
                     /locus_tag="VFI43_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060793.1"
                     /translation="MIKRAIQAGTSALVLAAAIYSSGAAAHGRVAMEEDTCMRRLGDN
                     SMVHLSAYQPQHEPSDQYCTDIPKGGNTFLVVDLVDPALRDIPIAMRVVRGTSELDGE
                     TVTYVNPSYHPDGVIRGETSLDKGQYTLLIEGQAVPPVRYQYPLRVEMINYAKTFRAA
                     VWPLIGMLL"
BASE COUNT         2316 a         2827 c         2851 g         2359 t
ORIGIN      
        1 cccgacgact cacgcctttc tcggccaggt tctcgatgcg aacaatcatc cccttatgat
       61 tgatggctta tgggcgatcg gccacggaaa tgggggagcg gcgggaaaca cgaacctgct
      121 ctacttcact gcgggaccca atgatgaaag ccatggatta ttcggagtgc taacgcctgt
      181 tccggagcct tcagaatata cgatgatgct cgccggactc ggcgctgtgc tgcttctgcg
      241 gcgacgccgt tctggcagcg atgtgggcga caaggcaaga gttcaggttt gacttttctc
      301 cagatcgacg gtttaaagac tcaagccctg ctcgttgagc agacctgcag ggcaatttta
      361 accagtaact cgaaaccccg tggcgggttc gatttcggag gtttttctat cgacctttta
      421 tttttctagc gaccgttgta acacatatct ggtcggctca cacctcccaa gccgccccgt
      481 tggccatacc cagccttcat tccccccctc ccgttctctt gtcccgatgc gcgagtacca
      541 gaccggcgag ctcgactcca acccccacgc aaaatagcag aacgccgatc cagatatttt
      601 ccggtgcaag gagcatcagc agtgctccca gaatgagcaa cggaatggat aacattcgct
      661 tcgtctgcgg atttcgtatc atatacaggg gaaacgcgtc gttgataaca tactatcaat
      721 atagacttca gccgtgaaaa ttgatggctg aaggcgtaat tgaggttccc tcacttcgta
      781 cggtatagat tcaaaggagg gtctctcttt cgaaaactcg tcgcatagtt atagcaaatt
      841 gtgaacaata aatcgtaacg aaatctaatg aaatattttc ttaatttgga agagcgcctc
      901 gccaatctgc ctcacccggt ctattccgac gatttgccgg ttgttgcccg cagggaagag
      961 atcgcgcggg caattggcga aaaccaggtg gtgatcgttt gcggcgaaac cggttcgggc
     1021 aagacaaccc aacttcccaa gatttgtctc gaactcaaac gcggagccgc ggggatgatc
     1081 gggcatacgc agccgcggcg tattgcggcg cgcaccgttg cggcgcgcgt tgcatccgag
     1141 ctgaatagtc cagtcggcca tgccgtgggc tacaagattc gattctccga caaggtgagc
     1201 gcgcagacgt atatcaagct catgacagac ggcattctac tggcggaaac ccagggcgac
     1261 ccttcgctgc aagcctacga taccctcatc atcgatgaag cgcacgagcg cagcctgaat
     1321 atcgacttcc tgctcggcta cctcaagcaa ttgctccccc gccgccccga tctcaagctg
     1381 atcgttactt cggccacgat caatgcacgc cgcttttcgg aacacttcaa tgcggcacca
     1441 gttatcgaga tctcaggtcg aatgtatccg gtggaagtgc gttatcgcgc tttcgattca
     1501 gttcattcat ctggcggtgc tgatcttgct cgggatgccg gtcagcccga agcacgggag
     1561 aaaaccggag gcaaaccaga tgaaagggac gaaggggata tagggcaggc tattctcgac
     1621 gcggtggatg aggcggtgcg ggaaggcccg ggcgatatac tggtgttcct gccgggggag
     1681 cgggaaattc gtgaaatggc cgaggcgttg cgcaagcatg cgttcggcag gcccggacgc
     1741 caaatgggtg gcggcgggat actcggggtg gacatccttc cactgttcgc ccgccagtcc
     1801 tatgccgatc aggaacgggt gttcaagcca ggcggtagta gtgtccgtcg cgtcgtgctg
     1861 gcgaccaatg ttgccgagac ttcgcttacc gttcccggca tacgttacgt gatcgatacg
     1921 ggattggccc ggctgaaccg ctatagttac cgcaacaagg ttgaacagtt gcgcgtggag
     1981 aagatttcgc gcgcttccgc cagtcagcga gcgggacgtt gcggccgggt gatgagcggc
     2041 atttgcatgc ggctgtattc ggaggaggac tatctttccc ggccggagtt taccgacccc
     2101 gagattctgc gctcctcgct tgcgtccgtg atactgcgca tgaaggcctt aaggatcggg
     2161 gcgatggagg actttccgtt tctcgatccg cctctgcctc gcatgatcgc ggatggctac
     2221 cagcttctgg ccgagcttgg cgcggtggat gagagcaaca cactgaccaa ggtgggttgg
     2281 cggcttgcga agtttcccat cgaccccagg atcgctcgca tgatcctggc cgccaaggat
     2341 gagaactgcc tgagagaggt gcttgtcatt gcggctgccc ttagcatcca ggatccccga
     2401 gaacggccgt tcgagcgcca ggaagcggca gatcgggcac atcagcgctt ccaggacgag
     2461 cgctctgatt ttctcgctta tctcaagctg tgggaatttt acgatgagct gctgaagcat
     2521 aagaaatcga atcgaaaact ggttacggat tgccagatac atttcctgtc acatcgcagg
     2581 atgcgggagt ggcgggaagt tcacggccaa ttgcacgcat tggttttgga aaccggcctg
     2641 cgtcccaatg aaattccggc ctcctacgaa gaaatccatc gggcgctgct ggccggcttg
     2701 ctagggaaca ttggctttaa aaccgaggag gatggtgaat atctcggcgc tcgcggaatc
     2761 aaattctcca tatttcccgg ctcggtgttg aaaaaagcca aacccaaatg ggtggtggct
     2821 gcggaactta ctgaaacaac caggctatat gcccgggttg tagcgaaaat agatcctgcg
     2881 tggatcgaga agattgccgg cgggctatgc aagaagcagt actttgatcc tcattgggaa
     2941 aaagctaccg cacaggttgc tgctttcgag cgcgttacct tatatgggct taccatcgtt
     3001 cccaagcgcc gggtttatta cggagctgtc aatccgcgcg aagcacgcga aattttcatt
     3061 cgttccgcac tggtagcggg cgagtatgca acccgtgcgc cgttccttga acacaaccag
     3121 aatcttgtgg aaagcatcgc ggaactggaa cacaaggcgc gaagacctga cgtactggtg
     3181 gatgaggcag tgattttttc tttttatgac gatatcattc ctgatcgcat tgcaaatgga
     3241 gcagcatttg aaaaatggcg caagcaggct gagcgggtaa atccatgcct gctctacctg
     3301 aaccgggacg acctgatgcg ccatgctgcc aatggggtta ccgaagagcg gtttcccgac
     3361 agcgttatgg tggacgggga atccgtgccc cttacttatc gcttcgatcc aggacacgta
     3421 ctggatgggg tcaccgttac cgtaccattg cccctgctca acaagcttga gtccacgcgg
     3481 ttcgactggc tcgtcccggg attgatccgt gaaaaggtga cgtggtacct caaggctctt
     3541 cccaaacaga tccgccgcca tgtcgtcccg gtgccggact tcgtgactgc attccttgaa
     3601 tatcgggaat cgaagcctga agtggtgaat aaaactattc cgctcaccaa ggcgctggcg
     3661 aaatttatcc tgttcaagac aggcattacc gttccaccgg atatctggca ggatgaaaag
     3721 cccccgccac acctattggt gaactataag gtggtggacg acgccgggca ggagctggca
     3781 atgagccgcg accttgaaca gctaagggcg cagctcggcc aggctgcgcg gttgatgttc
     3841 gcaggagccg tggggcaggc gccgccagaa cgtgtcccca tcgaacgtga cgatataaaa
     3901 cgttgggatt ttggcgattt gcccgaaaaa atagcgttct cccatggggg caagaagctt
     3961 accggttttc cggccctggt tagcggcgaa gggaaggtgg caatccagct gttcgataca
     4021 gcagaggccg cgcaagcaag catgcgcggc ggggtacgcc agttgctgcg attcgagctt
     4081 ggagagcaga tgaagcaatt ggaaaaggat ctgtccggac agagccgctc cttgagccag
     4141 gcatttttgc aattgcacat gctcatcagg ccggatgcgc tgcgtgaaga catattaaat
     4201 gctatcgctg accgcgcctt tatcggtaat gacccattgc cccgaaccga aaaggaattc
     4261 gccgagcgga gacagcgtgc gagaactcgc cttccagcgg taaccgacgc tgttatccgg
     4321 acggtgagtg atgttgcgag ggacgttcag gctttgctga ccaggatttc cggaactgca
     4381 ggagcaacca gggtcggcca acccaagtct ctggtgctgc cagaggctgc ggcaagactg
     4441 aaagccgaac tgaacgagga attgcggaaa ctcatttacc ccggctttct tgaagcaacg
     4501 ccttgggaaa agctccccca ccttccccgg tatttgaaag gcatgctcct gcgcctggaa
     4561 aaatatccag gcaacccgga gcgcgaccag cgccacgcgc gtgctattgc agcgttgtgg
     4621 cagcaatacg aacagcgcct ggaaaagcac cgaaaatccg gaatcagcga cgccaccctt
     4681 gccgaattcc ggtggcagat cgaggaactg cgcgtttcgc tgtttgccca ggaactgaaa
     4741 acgccccagc cggtttccgt aaaacggtta cagaaattgt gggaagggat gaccgcgtga
     4801 gaatggagtc tcccgggaat caactgatat cgcattcaat tcgatagttt gccgccaacc
     4861 gtgcctcgcc ccccgggggt acctgtacta cgtcgagatc cgcgttggtg gtttcgacgc
     4921 acacaaagta ttcgtagtcg tcgtcattca aatccgccat ctccgatgat atttttaccc
     4981 atgggttcca taccaccgcg ctattgctgc ctttcgaatt gatcctgatc ctgcggtcaa
     5041 acgctttatc gtcaatgacc aattcggccg gaacgttccg gaagatgcgg tcaacttcct
     5101 tgtcgatggt cactgggccc gcctgaaact tgatggcgcc attatctgtt ttatcaacat
     5161 acgcggcccc tgccagccct gaaatgatga cctggctgat atccccaacc ctgaagtaag
     5221 tatgcagcgc ctgagtaatg gaaaacgttt cactcgtcgt gttgcgcgtg atcaattcca
     5281 ggttcagagt gtctccaacc gtgatttcga gcacaaggtt gaatgagtgg ggccaaaggc
     5341 cacgcgtttc ctctgaatcg ttcagtctga gcgtgacctt gccttcctct tcaggagttg
     5401 cttctgttcg cacgacctcc cagaagcggt tgcgtgcaaa gccgtgtaac ggacggcctc
     5461 tcccttcagg atggggtccg aaccagggcc agcaaaccgg ggtgccgccc ttgatcgatt
     5521 tgcctggttg ataatatgct ttttcactga ggaacatgag gttgtgcggc tcattagcag
     5581 gctggtagga caagacctgt ccgccgtata ctgagatgat ggcgcttgct ttcgcattat
     5641 cgacccgaat ataggggaat ccacctgtcc cctcgatgaa tttgatgtga tcggtaatcc
     5701 cgtgctcatt gttcaactgc gaaacgttca tcgtgctgcc ctcccctttt gcaaagccgc
     5761 caacaccggc aatcccgcct aaccagcgcg gatagtattc cattatatgg caggcccgcg
     5821 tcctcgctga aaaaggtatt gattggtgct ggtgaacaaa aattgcctcc aactccagtc
     5881 gggccaagtt gtacgaaaaa caagcattga acaacggctg ccggtatagt atccacgtta
     5941 ccatcccgga ataccttccg cgaaccttca cagcggccaa ataaagcagc gaaagtttta
     6001 gtcttgccat ttcaataaat ggagcccttc tgagcaatca aacatcctct tcacaaaaga
     6061 agcagtcatt ctggaaaaag ctcggcccgg ggctcatcac tggcgcagct gacgacgatc
     6121 caagcggaat cgccacctat tcccaggttg gcgcgagctt cgggtattcg atgttgtggg
     6181 caactttttt tacctttccc ctcatggtgg ggatccagat cattagcgcg cgtattggcc
     6241 gtgtaaccgg cgagggcctc gccgcaaaca ttcgcaagcg ctacccctcc tggcttctgt
     6301 atggaatcgt ctgcctcgtg ctgatcgcaa acacgatcaa catcgctgcc aatattggcg
     6361 caatgggcgc cgcacttaaa cttctcattg gtggtcccgc ccatctgtat gccgtgttgt
     6421 tcgcgctcac gtcgctcgtt ctgcaaatct tcattccctt tccaagatat gcacccattc
     6481 ttaaactgct gacattctcg ctgtttgctt acgtggggac ggtgttcgtg gtgaacgtgc
     6541 cgtggggaga agtgctctac aagacgatac tgccgtcaat ctcactagag gcagagtacg
     6601 cggtcgcggt cgttgctatt tttggcacca ctatcagccc gtatctgttt ttctggcaag
     6661 cctcgcaaga agtcgaagaa caacaggcag cgcaaggcga agaacccctg aagaaagcgc
     6721 ctgaacaggc tgaagataac ttgaaaagga tcgatatcga tacctatgtc gggatgggct
     6781 tctccaacct cattgccttt ttcattatgc tgacggcagc agtaaccttg aatctcaatg
     6841 gcgtgacgga catcgagacg actgagcagg cggcggaagc gctgcgcccc atagcaggcg
     6901 aatttgcatt cctgctgttc tcggggggta ttgtggggac aggattgctg tcagtgccgg
     6961 ttcttgctgg ctccgctgct tacgccgtgg cagagtcgtt caactggcgc atcggcttgg
     7021 gccgcaagct gttggaagca agaggttttt acgccatcct cagcgtcgcc acgctcatgg
     7081 gagtcgcgct caatttcaca cccatcaatc cgatcgacgc gctcttctgg agtgcggtga
     7141 taaatggggt tatcgccgtc cccatcatgg cagtgatgat gttaatggcc acgagagccg
     7201 acgtgatggg accattcgtt gtaacaaccg gccttagaat tctcggctgg ctcggaacgg
     7261 gcgtgatggc gcttgcagtc cttgcgatgg ctttgacctg gaattcggga acgtagatcc
     7321 agccgccagt tccgctcaga caatttcgcc gtgctccgtt cgctgtgctc gttgtcatct
     7381 attgtttgcc cgtcaacgca gcagaatcag cctcgggatt gcgtccgcgt tggcccatag
     7441 tacagcgttt tgctggaact cagcgcccag cgtacgggac acttccagat ccaggccgag
     7501 tgcaagaaag ctttcttctc ccggccaatc tcctgaagga tcaagcccca ttccttcgat
     7561 gatgtcatcc ggaccggata cgtattgatt cagcctttcg cggagccgtt cgcacgccgc
     7621 ccggttttcc tgtggcctct ggcgaacgcc ccctgggttg caagccgtga taaacgcggc
     7681 accccgatgg tcagaagcgg aaaaaagccg agagagcgat tcagagtaat gatcgacacg
     7741 cagagtcact gaattacgtc caatccctgc ctggtagtgg gcggagcggt aagcatcgat
     7801 gacatcggca ggaattttcg actcctgctt ctcaaaacca ggctgctcga acctctggtc
     7861 accccagtga ggggtttcgg cagatttttt catatctcgc gtccgcttcc agtgatgagt
     7921 ctcacactgc gatcggaggt gaaataagac cagagccacc cgagcatgac taccatgcga
     7981 tttcgaaaac cgatcaggaa aaaaatatga acgagacccc atagcagcca tgcgacaaaa
     8041 ccactgaggc gcagccaccc gaaatccagt actgccgact tgtgcccgac ggtggcgagg
     8101 ttgcctgcgt ggtgataatg aaagacaggg tgaattcgct cgcgattgct tcctatgtcc
     8161 ccggctgcat ttattcgctg caatagcagt tccgcgacga acttgccttc ctgtttggcc
     8221 gctggcgcga tccctgggac aggttttccg tctggtcccg ttactgctgc cgtgtcaccg
     8281 atgacgaaaa ttgacgaatc gcctggcagt gacaagtccc cttgcacacg gactcgtccc
     8341 gcccgatctg gctcaacatc aagccactgc gcggctggag atgcggccac gccggctgcc
     8401 cagatcaagg tgcgtgcttc aatcctgcgt cccccggcaa tgaccccggc tgcatcgcaa
     8461 tgctcgactg ctgagccagt caatacctct actcccaggt gctcgagtga ttgtctggcc
     8521 ttttcggcaa gctcgggcac gaaaacgggt aatatctggg gaccagcctc gatcaggata
     8581 atgtgagcgc ttaagccttt caacgttcga gaggcctgcg cgagctcgat ctttgccaat
     8641 tcggctattg caccggcaag ctcgaccccg gtggggcctc cgccaataat cacgaaactg
     8701 agcagacgtt tttgttcttc agtgtccttt gtttgctcgg cgcgttcaag tgcatcgaga
     8761 acgcgattac gaatgtccag ggcgtcctcg atctgcttaa gtcccggtgc gaacgcctcc
     8821 cagtcgtcgt gaccaaaata ggagtgccgg gcgccggttg caacaaccaa aaagtcgtag
     8881 gggataatgc gagcgttttc gcgcagacga acagtcttgg cctgccggtt aacggcctcg
     8941 acgtggccca gaattacttt ggcattgagc tgatagcgca ggatgcggcg aatcggcatt
     9001 gcgatatcgg cgggagagag tgccgcagtg gcaacctggt acagtaaggg ttggaagaga
     9061 tggaagttgc gctgatcaac cagtgtcaac tcggctgagg cgttgcgcag cgcctttgcg
     9121 caggtcagtc cgccaaaccc ggcgccgagg atgacgatgc ggggtaattg cgagccttgg
     9181 ctttgcggct gttgccccac ttataaaaac cctcggagta cctgcattcc gtctcgcgct
     9241 cacgttgtct ggattgatat ccggatgcaa tcgtccagag aagtttgcct ttcaccagcc
     9301 agcccaaatt ggacaatcag gattcaacat aggctattga aaggaggatt gcctggaaat
     9361 agcgacgcgc tttgctgcca gtagcggccc ttatttctca tgtaatactt tatgaacgtc
     9421 ctataactca aggatggtat atattttata caactcatgt ttatttgagt ttagagccaa
     9481 ctgccgtcat ggagccataa ggaatcttct catcgttccc gtctttttcc gctctcggca
     9541 tcgaacgccg acctatcgtg tagctcgacc gcatatccag tttctctccg aaattgcgat
     9601 tgcatttctt ttttgttgca ttaggtaatt aagagattat actttcattg tgcaactata
     9661 gacgacgata acctgtccat aactccctgt cggcaggttg aattcaccta aaatttaggg
     9721 cgccggcgca atcctgtcgg ggcgttccct atattgctga ggcaaagatt ttcacacacc
     9781 tggccttgcg agggtatcga tcaatatcaa cgcgaaggca atcaacatat cggaggagac
     9841 agtcgaaatg atcaagcgag cgattcaggc tggcacgtcg gctcttgttc ttgctgcggc
     9901 gatatacagc agtggcgcgg cagcgcatgg aagagtagcc atggaagaag acacctgcat
     9961 gcgccggctg ggcgacaaca gcatggtgca tttgagcgcc tatcagccac agcacgagcc
    10021 gagcgaccaa tattgtacgg atataccgaa gggggggaat accttcctgg ttgttgatct
    10081 cgtcgacccg gcgctacgcg atatacccat tgcaatgcgc gtggtaaggg gtaccagcga
    10141 actggacggg gaaacggtga catacgtgaa cccaagctat catcccgatg gcgttatccg
    10201 gggcgagacg agtcttgata aagggcaata caccctcctc atcgaaggac aggccgtgcc
    10261 cccagtccgc tatcagtatc cgctgcgggt agagatgatc aattacgcca agactttccg
    10321 tgctgctgtc tggcctctca ttggaatgct tct
//
LOCUS       DASSHJ010000025         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1290824,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000025 DASSHJ010000000
VERSION     DASSHJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290824"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VFI43_01290"
     CDS             <1..1347
                     /locus_tag="VFI43_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET7060794.1"
                     /translation="HQDFSEDTYRVLTAVDAALMVIDAANGVEAQTLRLLEVCRARNT
                     PILTFVNKMDREVREPLDLIDEIERTLRMSAVPFTWPMGMGRGLKGVFDLQRDRMRVF
                     QPGEDKLNDDAEIIEGLDNPVIDARFPDLAEATRQEIELIRGASAAFDHAEFLAGKQT
                     PIFFGSAINNFGVREVLDALVELAPPPRSRKAFQRQVQPGETRFSGVVFKIQANMNPA
                     HRDRIAFVRVCSGRFERGMNLRVARNGKDLRTNSVVSFLSQRRELLETAYPGDIIGIP
                     NHGTLQLGDTLTEGECLQFTGLPFFAPEIFQTVEIADPMRSKQLKLGLVQLGEEGAIQ
                     VFRPHAGGSLLLGAIGTLQFDVVAHRLKHEYGVEARLMPAKYQLARWITSRNSRELKH
                     FIEVNAHRVAYDAVDAPTFLASYGAELSVAEENWEAIQFHKMREHAGLVFETHANG"
     gene            1537..2964
                     /gene="argH"
                     /locus_tag="VFI43_01295"
     CDS             1537..2964
                     /gene="argH"
                     /locus_tag="VFI43_01295"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7060795.1"
                     /translation="MTTSISDETPAPSGKGAWSGRFSEPVSELVQRYTASVGFDHRLA
                     EYDIQGSLAHASMLAAQGIITTEDFLAITRGLSQIREEIRSGDFIWRIELEDVHLNIE
                     KRLTALIGDAGKRLHTARSRNDQVATDIRLYLRHSIDGIINLIVEMQRALLDLAEKHA
                     DTLMPGFTHLQVAQPVVFGHHLLAYFEMLKRDAERFLDCRKRLNKLPLGAAALAGTSY
                     PIDRMMVAEQLGFEGPCENSLDAVSDRDFAIEFCAAAALVMTHLSRLSEELILWMSPP
                     FSFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRINGHLVALLTLMKAQPLAYNKDN
                     QEDKEPLFDTVGTLTDTLRIYADMMTGINVNKDAMRAAAMKGYATATDLADYLVRKGT
                     PFREAHEAVAQAVRFAAETGRDLSALALFELQKFSRLIDNDIFSVLTLEGSINSRNHI
                     GGTSPDQVRAAIRRGRDWMSGRHHP"
     gene            2974..>3274
                     /locus_tag="VFI43_01300"
     CDS             2974..>3274
                     /locus_tag="VFI43_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET7060796.1"
                     /translation="MVSWTPIAAQISGSTGSTFTIEKASPVAGGCINQAYKVEGKVEG
                     GGRTFFVKVNDPGSLAMFEAEADGLGELYNSNTLRVPAPVCWGADSSAAWLVLEYL"
BASE COUNT          729 a          848 c          935 g          762 t
ORIGIN      
        1 caccaggact tttcggaaga tacctatcgc gtcctgaccg cggtggatgc tgcgttgatg
       61 gtaattgacg ctgccaatgg cgtcgaggca caaaccctgc gactgctcga ggtatgccgg
      121 gctcggaaca caccgattct aactttcgtc aataaaatgg atagagaggt gcgggaaccc
      181 ctcgatctta tcgatgagat cgaacgaacc ttgcgcatga gcgccgtccc ctttacctgg
      241 ccaatgggta tgggtcgggg gttaaagggc gtatttgacc ttcagcggga ccgcatgcgt
      301 gttttccagc caggggagga taaattaaat gacgatgctg agataattga agggctcgac
      361 aatccggtca ttgatgcacg ttttccagac ttggctgaag ccactcgcca ggaaattgaa
      421 ttgatacgtg gggcgtccgc cgcattcgat cacgcggagt ttctcgccgg caaacaaaca
      481 cctattttct ttggttcggc gataaacaac ttcggggtac gcgaggttct tgacgcgctg
      541 gtcgagcttg ccccgcctcc acgatcgcgt aaggcgtttc aacgccaggt gcaaccggga
      601 gaaacccgat tttctggggt ggttttcaag atacaggcaa atatgaatcc ggctcaccgg
      661 gaccgcattg cttttgtgag agtgtgctcc ggaagattcg agcggggcat gaatctgcgc
      721 gtggcgcgca acggaaagga cctacgcaca aactcggtcg tatcgttttt atcccagcgg
      781 cgcgagttgc tggagaccgc ttatcccggc gatatcatcg ggattccaaa tcatggaacg
      841 ctgcaacttg gagatacgct cacggagggt gaatgccttc aatttacagg cttgcctttt
      901 ttcgcaccgg aaattttcca gacggttgaa atcgccgatc ccatgcgcag caagcagctc
      961 aagctggggc ttgtccagtt gggtgaagag ggcgcaatcc aggtcttccg gcctcacgcc
     1021 ggtggctcct tgttgcttgg ggcgatcggt acgttgcagt ttgatgtggt ggcgcaccgt
     1081 cttaaacatg aatacggggt agaggctcgc ctgatgccgg caaaatacca gctggcccga
     1141 tggatcacct ccaggaattc gcgtgagctg aagcatttta tcgaggtcaa tgcccatcgc
     1201 gtggcgtacg acgccgtcga tgccccgacg ttcctggctt catatggtgc ggagctcagc
     1261 gtagccgagg agaactggga agctatccag tttcataaaa tgcgagagca cgccgggctg
     1321 gtctttgaaa ctcatgcgaa tggttagcat gggcaggaga tgacgcctca gctttatcgc
     1381 tgtttttgtt tatactgatc gttcaaatat ccccaatggc agcattattc ccgcgatccc
     1441 tgcaaaaagc atgaagagca aggaccgtct attgctgccg ccccggattt atacggattt
     1501 ggggttttat atcgatccgg ggtttttata tggattatga caacttctat aagtgacgag
     1561 acccctgctc cgtcggggaa gggtgcctgg tccggccgct tcagcgaacc ggtttccgag
     1621 ctggtacaac gctataccgc ttcggttggt tttgaccaca ggcttgcgga gtacgatatc
     1681 cagggctcgc tcgctcatgc gagcatgctt gccgcccagg gaatcataac cacggaagat
     1741 tttttagcca ttacacgggg cttgagccag atccgggaag aaatacgtag cggtgacttt
     1801 atctggcgaa tcgaactgga agacgtgcat ctcaatattg agaagcgcct taccgcgctg
     1861 attggggatg ccggcaaacg tctgcatact gcacgctccc gaaacgatca ggtagccacc
     1921 gatatacggc tgtatttgcg acattccatt gacgggatta tcaatcttat cgtcgaaatg
     1981 caacgggcgc tgctggattt ggccgagaag catgccgata ccttgatgcc tggtttcacg
     2041 catctccagg tcgcgcagcc agttgttttc ggccaccacc tccttgccta ttttgaaatg
     2101 ctgaaacggg atgcggagcg tttcctggat tgccgaaaac gcctgaataa acttccactt
     2161 ggcgcggctg ctttggcggg aacgagctat cccatcgatc ggatgatggt agcggaacaa
     2221 ctgggattcg aaggcccatg cgagaactcg ctcgatgcgg tttccgacag ggatttcgca
     2281 atcgaattct gcgctgccgc ggcgctcgtc atgacgcacc tgtcgcggtt atcggaagag
     2341 ctgattttat ggatgagccc tccgtttagc tttattgatc tggctgaccg tttttgtacc
     2401 ggttcgtcca tcatgcctca aaagaaaaac cccgatgtgc ccgagttggt tcgaggcaaa
     2461 acgggacgca tcaacggaca tctggtcgca ctgctgacac tgatgaaagc tcagccgctc
     2521 gcgtacaaca aggataatca ggaagacaag gagccgctct tcgatacggt gggtaccctt
     2581 accgatacgc tacgtattta cgccgacatg atgacgggca taaacgttaa caaggacgcg
     2641 atgcgagcag cagccatgaa gggttatgca acggcgaccg atcttgccga ttacctcgtc
     2701 agaaaaggca cgccctttcg cgaagcgcac gaagcggtag cccaagccgt gcgctttgcg
     2761 gccgaaaccg gtcgcgacct cagcgctctc gccttgttcg agttgcagaa attttctcgg
     2821 ttgatcgaca acgacatctt ttccgtattg accctggagg gatcgatcaa cagccgcaac
     2881 cacatcggcg gcacctcacc agatcaagtg cgtgccgcaa ttcgccgtgg gagagactgg
     2941 atgagcggtc gacatcaccc ctagtttgcg ttcatggtct cctggacacc catcgctgca
     3001 caaatctccg gttcgacggg gtctacgttc actatcgaaa aagcctcccc cgttgcgggg
     3061 ggttgtatca atcaggccta caaggtagag ggaaaagtag aaggaggggg gcgcacgttt
     3121 tttgtgaagg tgaacgatcc gggaagtttg gcgatgttcg aagcagaagc ggacggtttg
     3181 ggggagttat acaattcgaa taccctccgt gtgcctgccc ctgtgtgctg gggtgcggac
     3241 agttccgcgg catggcttgt cctggaatat cttg
//
LOCUS       DASSHJ010000026         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1620485,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000026 DASSHJ010000000
VERSION     DASSHJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620485"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1410)
                     /locus_tag="VFI43_01305"
     CDS             complement(64..1410)
                     /locus_tag="VFI43_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7060797.1"
                     /translation="MLVTLLAPCAAFASAHEFTLANGLKLVVKEDHRSPVVISQIWYK
                     AGSTDELNGTTGVAHVLEHMMFKGTQKVPGGEFSRRIAAAGGRENAFTSRDYTAYFQQ
                     LQKSKLPLAMELESDRMRNLVLSEEEFAKEIRVVMEERRLRTDDQPRALVHEKMLATS
                     YQSHPYRHPVIGWMNDLENMTVRDAKDWYDQWYAPNNAVLVVVGDVDPKEVYALAQRH
                     YGPIKRRPLLPLEKRKPQTEPPQVGIKRILVKAPARLPYIAMGYHAPVLNKPATEWEP
                     YALEILAGVLDGNSSARLNKALVREQHIASSAGAGYDSTSRGPGMFYLDGTPSEGKTA
                     ADLEAGLRKEIEKLIHEGVTEEELVRVKAQVVAGHVFQLDSMFFQAMQIGQLESIGLS
                     HRDVDTMLKKLQAVTAEQVRHVARKYLKDDGLTVAVLDPQPLEQKTFAAPPTGLRH"
     gene            1593..2579
                     /gene="ftsY"
                     /locus_tag="VFI43_01310"
     CDS             1593..2579
                     /gene="ftsY"
                     /locus_tag="VFI43_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7060798.1"
                     /translation="MPSFFKSDNNSSAPDPTTESWASRMRAGLSRTRNSLGRQISGLF
                     GGGKIDEALYEELESILLTSDVGMDATLWLLEELRRKVKRENLSDASQLKGALQQVLI
                     ALLEPLAQPMETENHKPFVIMLAGVNGAGKTTTIGKLAHYFQLQGKTVLLGAGDTFRA
                     AAREQLLAWGERNNIAVIARESTAQQKADPAAVIFDAVNAAKARKIDIVLADTAGRLA
                     TQLHLMEEIKKVKRVIAKAEPGAPHETLLVLDANTGQNAISQVKAFDEALGLTGLIIT
                     KLDGTAKGGVIAAIARQRHGQSILPIRFIGVGEGLNDLRPFVASEFVEALFD"
     gene            2621..3277
                     /locus_tag="VFI43_01315"
     CDS             2621..3277
                     /locus_tag="VFI43_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7060799.1"
                     /translation="MISFSQVYKRYPNGFEALRNISFELEAGEMAIVTGHSGAGKTTL
                     LKLIAAIERPTSGSIVVNGQNVGKLRRGAIPFLRRNLGLVFQDQKILFDRNVFNNVLL
                     PLQISGFGSKAAAARVRAALDKVGLLNKEKARPITLSGGEQKRLCIARAIVNRPSILI
                     ADEPTANLDADYSKAIMDMFRSFHQVGVTVLIATHDPGLLGDDIQQRVFSLQHGELPA
                     "
     gene            3274..>3563
                     /locus_tag="VFI43_01320"
     CDS             3274..>3563
                     /locus_tag="VFI43_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7060800.1"
                     /translation="MKAWVSHHLHAFAMAFKRLGTAPLGSLLSIGVMGIAFSLPAGIY
                     VMLENLQGFSGEFSAAPQLSLFLKPEASAKDVAEIESSLKQNPGVAGFRFVP"
BASE COUNT          804 a          982 c          997 g          780 t
ORIGIN      
        1 gtgcgtagag gcagcggaac tggagaaaaa cttctgttcg atcaagaagc cgggcgcata
       61 gggttaatgg cgcagtccgg tgggcggcgc ggcaaacgtt ttctgctcca atggttgagg
      121 gtcgagcacc gcgacggtca acccgtcatc tttcagatat ttcctcgcaa cgtggcgcac
      181 ttgctcggca gtgaccgcct ggagcttttt aagcatcgtg tcgacgtcgc gatgggagag
      241 cccgatactt tcgagctgtc ctatttgcat cgcctggaag aacatcgagt caagctgaaa
      301 aacgtgacct gccacgacct gcgccttgac gcggacaagc tcctcctcgg tcacgccttc
      361 atggatcaat ttctcgattt ccttgcgcag cccggcttca aggtctgcag ccgttttacc
      421 ttcgcttggg gtgccatcaa gataaaacat gcccggacca cgggaagtgg aatcgtaacc
      481 tgcaccggct gaactcgcga tgtgctgttc gcgtaccagc gccttattga gacgggctga
      541 actattgccg tccagcacac ccgccaggat ctcgagcgcg taaggttccc attcagttgc
      601 cggcttgttc agaaccggag cgtgatagcc catggcaatg tacggcaggc gggctggcgc
      661 cttgacaaga atacgtttga tcccgacctg gggaggctcc gtttggggct tgcgcttttc
      721 gagaggcaat agcggccggc gcttgatggg gccgtaatgc cgctgggcga gcgcatacac
      781 ttctttcgga tcgacatctc ccaccaccac cagcacagca ttgttgggcg cataccactg
      841 gtcataccag tcctttgcat cccggacggt catgttctcc aggtcgttca tccagccaat
      901 tacagggtgg cgataaggat gagattggta ggacgtcgcc agcatctttt cgtgcacaag
      961 cgcgcgaggc tggtcatcgg tacgcaaacg ccgttcttcc atgacaaccc ttatttcctt
     1021 ggcaaactct tcttccgaca ggacaaggtt gcgcatccgg tccgactcca gttccatcgc
     1081 cagcggcagc ttcgattttt gcaactgctg aaagtacgcg gtgtagtcgc ggctagtaaa
     1141 ggcgttttcg cgcccgcccg ccgctgctat acgcctggag aattcgccgc cgggaacctt
     1201 ctgcgtccct ttgaacatca tgtgctccag cacatgagcc accccggttg tcccgttcag
     1261 ctcatcggtg ctcccggcct tgtaccatat ctgggaaatg actacaggtg agcggtgatc
     1321 ctctttgaca accagcttta gaccgtttgc caaggtaaat tcgtgcgcgc tggcaaaggc
     1381 ggcacaggga gccagcaaag tgaccagcat cgcaaatccc gaatggacat aaaaccgggc
     1441 tgcgcgacga aaaggcgtga ttttcataac taaaaaccgg gtggggaaat aaaatattcg
     1501 aataaaatga gcagataata ccgatgggct aaaatgagtt cttttgcatc aagttaagca
     1561 tgagtctcac ttgaagttga cagagtaaca gaatgccgag tttcttcaaa tccgacaata
     1621 actcttccgc ccccgatcca acaaccgaaa gttgggcctc gaggatgcgg gcgggcctgt
     1681 cgcgcacgcg gaatagcctg ggccgtcaaa tctcggggct gtttggcggc ggcaagatcg
     1741 atgaagcgct ctacgaggag ctggaaagca tcctgctgac ttcggacgtt ggcatggatg
     1801 cgactctctg gctgcttgag gagctgcgca gaaaagtcaa gcgcgaaaat ctttcagatg
     1861 cgagccagct caagggtgcg ttgcagcaag tgcttatcgc tcttctggaa cccctggccc
     1921 agccaatgga aacggaaaac cataagcctt tcgtcatcat gctggccggt gttaacgggg
     1981 caggaaagac cacaaccatt ggcaagcttg cacattattt tcagttgcag gggaaaaccg
     2041 tgctcctcgg agcgggagat acgtttcgcg cggcggcgcg cgagcaactg ctcgcctggg
     2101 gcgaacgaaa taatattgct gttatcgcac gggaaagcac ggcccagcaa aaagcggacc
     2161 ccgcggcagt catattcgat gcagtcaatg ccgcgaaagc gcggaaaatc gatatcgtac
     2221 ttgcagacac tgctggccgg ctcgccacgc aactccacct gatggaagaa ataaaaaaag
     2281 tgaagcgtgt cattgcaaaa gccgaacctg gagcgcccca cgaaacgcta ctggtgcttg
     2341 atgccaacac gggtcagaac gcgataagcc aggtaaaggc attcgacgag gccctcggcc
     2401 tcaccggcct cattatcacc aagctggacg gcaccgccaa gggtggcgtt attgctgcca
     2461 tcgcacgcca acgccatgga caatccatcc tgccaatccg cttcatcgga gtgggggaag
     2521 gactcaatga cctgaggccg ttcgtggctt cggaatttgt cgaggcgctc tttgactagc
     2581 ggcgatcatg gctggcgtcg gttggcggca ttgaaattta atgattagct tcagtcaggt
     2641 ttacaagcga tatcccaacg gttttgaagc gctcaggaat atcagcttcg aactcgaggc
     2701 tggcgagatg gccatcgtca ccggccattc cggcgctgga aaaacgacct tgctcaagct
     2761 tatcgcagcc attgaacgtc cgacttccgg aagcatcgtg gtgaacgggc agaatgtcgg
     2821 caagctcagg cgtggtgcga ttccttttct tcgccgcaac ctcggcctgg tgttccagga
     2881 tcagaaaatc ctgttcgatc gaaatgtatt caacaacgtg ctgcttccct tgcaaatcag
     2941 cggtttcggt tcaaaggcgg cggcggcgcg ggtacgcgcg gcacttgata aggtagggct
     3001 attgaataag gagaaggcac gtcctatcac gctctctggc ggagaacaga agcggctctg
     3061 catcgcgcgc gctatcgtca accgtccttc catactcatc gcagacgaac caacggcgaa
     3121 tctcgacgcc gattactcga aagccatcat ggatatgttc aggtcatttc atcaggtcgg
     3181 tgtcacagtg ctgatagcga cccatgatcc ggggctgctt ggcgatgata tccagcagcg
     3241 cgttttttct ctacaacacg gggaactgcc cgcatgaaag cgtgggtttc ccatcatctt
     3301 catgcttttg ccatggcgtt caagcggctc ggtacagcgc cgctgggaag cctcctcagc
     3361 attggcgtaa tgggaatcgc tttcagcctc cccgccggga tttatgtcat gcttgaaaat
     3421 cttcaggggt tttccggtga attttctgcg gctccccagc tgagcctttt tcttaaaccc
     3481 gaggcgagcg cgaaggatgt agcggaaatc gagtcaagcc tgaaacagaa tccgggagtc
     3541 gccggtttcc ggtttgttcc cag
//
LOCUS       DASSHJ010000027        11657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1626953,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000027 DASSHJ010000000
VERSION     DASSHJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626953"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="tsf"
                     /locus_tag="VFI43_01325"
     CDS             <1..738
                     /gene="tsf"
                     /locus_tag="VFI43_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7060801.1"
                     /translation="AAEGMVGAYISPDRKHGALVEVNCETDFVARNEDFINFVRSVAQ
                     LLTTENLADQEALSVAPLPGGEKVEDFRKALVMKLGENISIRRFVRYDTEGQLASYLH
                     GAKIGVMVDYNGGDEALGKDLAMQIAASKPICVSGDQVSPELLARERAIYGAQAAESG
                     KPADIVARMVDGRIAKYLAEVTLLGQPFVKNPDQTVEKLLASKSAKVNGFTIYVVGEG
                     IERKSDDFAAEVMAQVNQAKEDKAQPK"
     gene            743..1459
                     /gene="pyrH"
                     /locus_tag="VFI43_01330"
     CDS             743..1459
                     /gene="pyrH"
                     /locus_tag="VFI43_01330"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7060802.1"
                     /translation="MTTAAYRRILLKLSGEALMGDDSYGINRGVIERIVSEIAEVTAL
                     GVQVAVVIGGGNIFRGMSSAQDGMDRATADYMGMLATVMNALALQDAMRRGGLTSRVQ
                     SALRIDQVVEPYIRGKAIRYLESGKVVIFAAGTGNPFFTTDTAAALRGMEMNVDIVLK
                     ATKVDGVYDSDPQLNFGAVRYRTLSFDTAISQNLKVMDATALTLCRDQKLPLNVFSIF
                     RPGALKRVVMGEDEGTLVEV"
     gene            1545..2102
                     /gene="frr"
                     /locus_tag="VFI43_01335"
     CDS             1545..2102
                     /gene="frr"
                     /locus_tag="VFI43_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7060803.1"
                     /translation="MIPDIKKSAEQKMQKSLEALKLDLGKVRTGRAHTGLLDHITVDY
                     YGTPTAINQVANVSLADARTITVAPWEKKMLGAIEKAIRNSDLGLNPMTVGELVRVPM
                     PALTEERRRDLTRIVKHEAETARVAMRNIRRDANANLKERLKDKKIAEDEERRGQDEI
                     QKLTDRYIAEIEKILQTKEAELMAV"
     gene            2237..3034
                     /gene="uppS"
                     /locus_tag="VFI43_01340"
     CDS             2237..3034
                     /gene="uppS"
                     /locus_tag="VFI43_01340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET7060804.1"
                     /translation="MPPHISSTREIPETSIIPRHIAIIMDGNGRWAKKRFLPRVAGHK
                     KGVETVRTAIRACVDRGVEYLTLFAFSSENWRRPSDEVAFLMQLFMHVLEQEVSKLHE
                     NGIRFKVIGDLSKFEPRIVDFIRNGEALTAQNTKFTLTIAANYGGRWDMLQAVRRMLE
                     QRPELANCFDEQDLAPHFALNYAPEPDLFIRTGGEKRISNFLLWQLAYTELYFTDTLW
                     PDFDAHALDLAIQSYQQRERRFGRTSEQLQKTPSIHGGREVAIKRTA"
     gene            3085..3906
                     /locus_tag="VFI43_01345"
     CDS             3085..3906
                     /locus_tag="VFI43_01345"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7060805.1"
                     /translation="MVRTRIITAVIMLCLFLAAVFYLRAVFWMVLLLALTVTGSWEWS
                     RLAQFSARHSIIYLLLTALLGGELLYLLDRAVNADAFSHSFLSFYLASLVFWGLIVPY
                     LLKSRYAIRSRLLLALTGWLVLLPTSFALYQLRAIDPIVLLGFMATVWVSDTAAYFAG
                     RAFGKHKLAPNISPGKTWEGVAGALAAVFIYALLWRHLAGEEAQPKFLIPVLLVIAVL
                     GIIGDLFESLMKRHAGLKDSGNILPGHGGILDRIDALTSTLPVAALSILFLRPEL"
     gene            3903..5090
                     /gene="ispC"
                     /locus_tag="VFI43_01350"
     CDS             3903..5090
                     /gene="ispC"
                     /locus_tag="VFI43_01350"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7060806.1"
                     /translation="MISRRHLTILGSTGSIGANTLDVVARHPDRFAVVALAANKSAKK
                     VLEQCRYFNPSYAVLLDQASAEWLAAEIAAAGLDTEVLWGVESLEKVSSLPEVDVVMA
                     AIVGAAGIRPTFAAARAGKHVLLANKETMVMAGRIFMDVVKENSATLLPVDSEHNAIF
                     QSLPHHFKGDLQRVGVRRILLTASGGPFRQAPLATLEHVTPAQACAHPNWVMGQKISV
                     DSATMMNKGLEVIEARWLFEAEPEQIEVVIHPQSIIHSMVEYVDGSVLAQLGNPDMRT
                     PIAHALAFPERIETGVIPLDMFKVGRLDFEEPDFKRFPCLRLAYQALATGGSAPAILN
                     AANEVAVESFLNGHMPFTSIPLMIEEVMQNIGPGDITTLNDVLVADRAAREAAYEWLA
                     CLA"
     gene            5390..6763
                     /gene="rseP"
                     /locus_tag="VFI43_01355"
     CDS             5390..6763
                     /gene="rseP"
                     /locus_tag="VFI43_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET7060807.1"
                     /translation="MATTFLFTLAAFIVALGLLILFHEFGHYLVARWSGVKVLRFSIG
                     FGKPLYTRRIGDDQTEWVISAVPLGGYVKMLDEHEGHVEPQDLPRAFNRKPVAYRFAI
                     VAAGPLANFLLAILLYWALFILGVTALKPVLGPVQPSTPAASAGFHAGETIRTVDAEP
                     VSAWQDVRWLLLSKVAGTSSSVTVETQDADGRRAARSLDLSQLTADDLEGDFLKKVGL
                     VPYQPAIKPIIASVSSGSPGERAGLQPGDEIVAVDGKKVTFWEDLVQDIRESPGRALS
                     LEILRNNRTVDLDVVPDTVSEGGKKTGKLGIAPKVDHAELEKLLTEVKYSPGEAIVKA
                     VSKTWEMSLFTLRMIGKMVAGEMSWKNVSGPITIADYAGKSAQMGITSYLGFLAVISI
                     SLGVLNLLPIPVLDGGHLMYYVLEMIKGRPLSARAMQVGQQVGMALLFALMIFAIYND
                     ISRLIPT"
     gene            6766..9048
                     /gene="bamA"
                     /locus_tag="VFI43_01360"
     CDS             6766..9048
                     /gene="bamA"
                     /locus_tag="VFI43_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET7060808.1"
                     /translation="MKVRTLVALVSAFCASISWAREPFVVKDIRVEGIQRTEAGTVFS
                     YLPVKVGDTLDDEKAAAAIKALYATGFFKDVRLESENGVLLVVVEERPAIAQITVVGS
                     KEFEKDKLKEGLKQAGLAESRIFDRSMLEKAEQELKHQYITRGKYGVKITTTTTPLER
                     NRVAINFHIDEGKTAKIRGINIIGNKSFKERDLLSAFALRTPGLLTWFTKEDQYSKQK
                     LSGDLEALRSYYLNRGYLEFSIESTQVSITPDMQDIYITVNITEGPKYSVSEIKLAGE
                     MLVPEADLRKLIKMKVGDVFSREHLTESVKLITDRLGDDGYAFANVNASPQLDKDKQQ
                     VAFTFFIDPGRRVYVRRINITGNSRTRDEVIRRELRQFESSWYSTSKINRSKQRVDRL
                     NYFNAVSIETPAVPGTTDQIDVNVEVKEKPTGAVMFGAGYSNMEGLILSGSIAQDNIF
                     GTGKFVNLMVNTGSVNKTYSLSYTDPYFTKDGVTAGLDLYKRTLNTSTLVAVNSFNTD
                     TTGAGLRFGIPINENDSIMAGLGVENVIIHLGDNSPQRNIDFVQRFGKSTLNIPLTLN
                     WRRDGRDSAIWTTSGTTQRVALESGLPGGDLTYYKVSYDLRWYYPLTETFTLMLNGEL
                     GYGAGYNGKELPFFKNFFAGGNTSVRGYNISSLGPRDVNNRTLGGNRRAVGNAEILFP
                     MPGMRNDRSVRLSAFFDAGSVWGPDGVVPRQEGLRYSAGIAVQWISPMGPLKISIAQP
                     LNSQPGDNIQRFQFQFGQQF"
     gene            9103..9648
                     /locus_tag="VFI43_01365"
     CDS             9103..9648
                     /locus_tag="VFI43_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET7060809.1"
                     /translation="MNNPEIKWEQPVRVPLFKTGLVVFAFIWGAGAATADEIKIGVVD
                     TERILAESAPAVRALKKIEKEFLPRDQELGKMASQAKALQETLEKDGKTMPEADRRSK
                     ERELANLNREYQRAQRQMREDLSLRQNDEVAALQLTATKAIQVIAETEKYDLILPLRD
                     LVYRSQRVDITEKVLKALADK"
     gene            9768..10205
                     /gene="fabZ"
                     /locus_tag="VFI43_01370"
     CDS             9768..10205
                     /gene="fabZ"
                     /locus_tag="VFI43_01370"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET7060810.1"
                     /translation="MDIHEILRYLPHRYPFLLVDRVLSCEPGKDIAALKNVTMNEPFF
                     NGHFPGYPVMPGVLIIEALAQAAGILTLRSTSVKPNEDSLFYFVGIDAARFKKPVQPG
                     DQLILKATILRERIGIWKYSARAEVDGRVAAEAELMCTVRNKQ"
     gene            10344..10955
                     /gene="rnhB"
                     /locus_tag="VFI43_01375"
     CDS             10344..10955
                     /gene="rnhB"
                     /locus_tag="VFI43_01375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7060811.1"
                     /translation="MGADDFIRVCGVDESGRGPLAGPVFAACVVLNPARPIEGLADSK
                     TLSEQARDRLTVLIKENSLAWAISSASVGEIDRLNILQASLLAMRRAVKSLSLVPGRV
                     LVDGNHRPRLSYPVTAIVRGDSLVPEISAASIIAKTARDAEMVALHRRFPHYGFDRHK
                     GYSTEQHIEALRIHGASVVHRRSFAPVRELISGAWKHENKEAR"
     gene            11050..11535
                     /locus_tag="VFI43_01380"
     CDS             11050..11535
                     /locus_tag="VFI43_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7060812.1"
                     /translation="MRIEKDTVVSLDYKLYDMMGKVVEESGEPVSYLHGGYDAIFPAV
                     EEQLNDKEVGYSCSVLMEPEDSFGEYDSELVRVESRELFPDNIAVGMQFEGTQEGSDD
                     TLIYTVTDIAEDKVVVDGNHPLAGVGLRFDCTVTAVRPATPDEVSHGHAHGPDGHGHD
                     H"
BASE COUNT         2711 a         2984 c         3311 g         2651 t
ORIGIN      
        1 gcggctgaag gcatggtggg cgcgtatatc tcgcctgacc gcaagcatgg cgcgctagtt
       61 gaggtcaact gcgaaaccga tttcgtcgca cggaatgaag acttcatcaa ctttgtgcgc
      121 agcgtggcgc agctattgac aacagaaaat ctcgccgatc aggaagcgct ctcggttgct
      181 cctctccctg gcggcgaaaa ggtggaagat ttccgaaaag cgctggtgat gaagttgggt
      241 gaaaatatca gcattcggcg cttcgtacgc tatgacaccg aggggcagct tgcctcatat
      301 ctgcacggtg caaagatcgg agtgatggtt gattacaacg gaggtgacga agctctcggc
      361 aaggaccttg cgatgcagat tgcggctagc aagccaattt gtgtctcagg ggaccaggtg
      421 tcgcccgagt tgctggcgcg agagcgcgcg atctacggcg ctcaggccgc cgaaagcgga
      481 aagcccgccg atatcgtggc gagaatggtg gatggccgga tcgccaagta tctggcggaa
      541 gtaactctcc tcggtcagcc ctttgtcaaa aatcctgatc agacggtgga aaaattgctg
      601 gcgtcgaagt cagcgaaagt gaacggcttc acgatatacg tggtaggtga gggcatcgaa
      661 aggaaatcag atgattttgc cgccgaggtg atggctcagg tcaatcaggc taaagaagac
      721 aaagcccagc cgaaatagct ccatgacgac tgcggcctat aggcgaatcc tgctgaagct
      781 ctcgggtgaa gcgctcatgg gcgatgacag ctatggcatc aaccgcgggg tgattgaacg
      841 gatcgtcagt gaaattgccg aggtaactgc cctgggcgtt caggtagctg ttgttatcgg
      901 gggaggaaac atttttcggg gaatgtcctc cgctcaagac gggatggacc gggcgactgc
      961 cgattacatg ggtatgctgg ctacggtaat gaatgcgctg gcgctacagg atgcaatgcg
     1021 ccggggaggg ctgacaagca gggtgcaatc agcgcttcgc attgatcagg tggtggaacc
     1081 ttatatccgc gggaaagcca tacgctatct ggaatcgggc aaggtggtaa tttttgcggc
     1141 gggcacaggg aatcctttct ttactactga tacggcggct gcgcttcgag gcatggagat
     1201 gaatgtcgat atcgtgctca aggctaccaa agtcgacggg gtatacgact ccgatcctca
     1261 gttaaatttc ggcgccgtgc gataccgtac gttatcgttt gacacagcta tttcacagaa
     1321 tctcaaggtg atggatgcaa ctgcgctcac gttatgccgg gatcagaagc tcccactcaa
     1381 cgtcttcagc atcttcaggc ccggcgcgct gaagcgggtc gtaatgggtg aagacgaagg
     1441 gacgctggta gaggtttagc acagccctcc cgaatgggga tggccgatat cgttccttta
     1501 aattccctta atcacggttt tatactttcg ttcactgaac tatcatgatc ccggatataa
     1561 aaaagtcagc cgaacaaaaa atgcagaaaa gcctcgaggc tctgaagctg gatctgggca
     1621 aggttcgcac ggggcgggca cacacggggc tgctcgatca tattacagtc gactactacg
     1681 gcacgcctac cgctatcaac caggttgcca atgtcagcct tgctgatgca cgtacgatca
     1741 ctgttgctcc ctgggaaaag aaaatgctgg gtgccatcga aaaggccatt cgcaattctg
     1801 atcttggatt gaaccccatg accgttggag aacttgtccg cgtgccaatg ccggccctga
     1861 cagaggaacg gcggcgtgac ctcacccgta tcgtcaagca cgaggcggaa actgcgaggg
     1921 tggcaatgag aaatattcga cgcgacgcga acgcaaacct gaaggaacgg ctcaaggaca
     1981 agaaaattgc cgaggatgag gagcgccgtg ggcaggatga aatccagaag ctgacagatc
     2041 gttacattgc ggagatcgag aagatattgc agaccaaaga ggccgaactg atggcagtct
     2101 gaacgcgact tcgccgtgaa gccccaaatc taatctggat tcttctgtca ttaaacccac
     2161 gagcgccttg attcgtttcc gggagcgctc gatgttctca actatgcgta ctcactgtga
     2221 gtgctcaact gtggaaatgc ctccccatat cagttcaacc cgcgaaatac ctgaaacaag
     2281 catcattccc cggcacatcg ccattatcat ggatggtaac gggcgatggg caaaaaagcg
     2341 gttcctgccg agagtggctg ggcataaaaa gggagttgaa acggtccgta ccgcgatcag
     2401 ggcctgcgtc gatcgtggag tcgaatacct caccctgttt gctttcagta gtgaaaattg
     2461 gcgaagacct tcagacgaag ttgcgttcct gatgcagctc ttcatgcacg tgctggagca
     2521 ggaggtcagc aagctgcatg agaatggaat ccgctttaag gttatcgggg atctgtcgaa
     2581 atttgaaccg aggatcgtcg attttattcg aaacggggaa gcgcttaccg cgcaaaacac
     2641 gaagttcacg ctgacgatcg ccgcgaatta tggtggccgc tgggacatgt tgcaggcggt
     2701 tcgtcgaatg cttgagcaac gccccgaatt ggcaaactgt tttgacgagc aggatctggc
     2761 tccccatttt gccttgaatt atgcgcccga acccgatctc ttcattcgaa ccggcgggga
     2821 aaagcgtatc agcaattttc tgttatggca gctcgcctac acggagcttt acttcacaga
     2881 cactctttgg cccgattttg atgcgcatgc attagacctt gccattcaat cctatcagca
     2941 gcgcgagcgc cggtttggcc gaaccagcga gcaacttcag aaaacaccgt ccattcacgg
     3001 gggaagagaa gttgcgatca agagaacagc ttaaccctcg cccttcttta atatcccctg
     3061 gtttcttgtc cggctgtagc aggcatggtt aggaccagga ttattaccgc cgttatcatg
     3121 ctatgcctgt tcctggccgc agtgttttac ctgcgtgctg ttttctggat ggtattgctg
     3181 ctggcgctga ccgttacagg atcatgggaa tggagcaggc ttgcgcagtt ttccgcacgg
     3241 cactccatta tttacctgct gctcacggcc ctgctgggcg gagaactgct gtacttgctg
     3301 gacagggcag tgaatgccga cgcattcagc cactcatttt tgagctttta tctcgcatcg
     3361 ctcgtcttct ggggactcat cgtcccttat ctgctcaagt cgagatacgc gatcaggagt
     3421 cgcctgctac tggccctcac cggctggctg gtgctactgc caaccagttt tgccctatac
     3481 cagttgcggg cgatcgaccc catcgtactg ctgggattca tggcgacggt ctgggtgtcg
     3541 gacactgccg cttatttcgc cggacgtgct ttcggcaagc acaagctcgc gccgaatatc
     3601 agcccgggaa agacctggga gggggttgct ggggcattgg cggcggtttt tatttatgcg
     3661 ttgctatgga ggcatctcgc aggggaggag gcacagccga agtttctcat cccggtgttg
     3721 ctggttattg ccgtactcgg cattatcggc gacctgttcg agtctctcat gaagcggcac
     3781 gccggactga aggacagcgg caatattttg ccgggtcacg gtggtatact tgaccgcatc
     3841 gatgcactga cgtcaaccct gcccgtggct gccctctcaa ttttatttct ccgccctgaa
     3901 ttatgatctc ccgccgtcat cttactattc ttggctctac cggaagcatt ggcgccaata
     3961 cgttggacgt ggtcgcgcgt catccggacc ggtttgcggt tgttgcgctt gctgcgaaca
     4021 aaagcgcaaa aaaagtgctg gaacaatgcc gctacttcaa tccgagctac gccgtgcttc
     4081 tggatcaggc aagcgcggaa tggctcgccg cagagattgc ggcggcagga cttgataccg
     4141 aagtcctttg gggagtcgag tcgctggaga aggtctcatc actgccggaa gttgacgtag
     4201 tcatggcggc gattgtgggt gctgccggaa ttcgtcccac gtttgcggct gcccgtgcag
     4261 gtaaacacgt gttgctggcg aacaaggaaa ccatggtgat ggccggacga atcttcatgg
     4321 acgtggtcaa ggaaaactcg gccaccctgt tacctgtcga cagcgagcac aatgcaatct
     4381 ttcagtcact acctcatcat ttcaagggcg atttgcaaag ggtgggagtt cgccgaatcc
     4441 ttctgacggc atccggagga ccattccgcc aggcgcctct ggcaactctg gagcacgtga
     4501 ccccagcgca agcctgtgcc catccgaatt gggtaatggg tcaaaaaatt tcggttgact
     4561 ctgccaccat gatgaacaag gggctcgagg tcatcgaggc gaggtggcta ttcgaagcgg
     4621 agcctgagca gatcgaggtg gtcatccatc cccaaagtat tattcattcc atggtcgaat
     4681 atgtggatgg ttcggtactg gcgcaacttg gcaacccgga catgcgcacg cctatcgccc
     4741 atgccctggc tttccccgaa cggatcgaaa cgggggtaat acccctggat atgttcaaag
     4801 tggggcggct tgattttgag gagccggatt tcaagcgctt cccttgcctc aggctcgcat
     4861 accaggcctt ggcaaccggg ggaagcgcgc ctgcaatact gaatgccgcg aacgaagtcg
     4921 cagtggaatc cttcctcaat ggccacatgc cttttacgtc cattcctctc atgatcgaag
     4981 aggtcatgca gaacattgga cctggagata ttacgactct gaatgatgtg ctggttgccg
     5041 atagagcagc acgtgaagca gcctatgaat ggcttgcgtg cctggcgtaa agcgttgcaa
     5101 ccaaaggttc tggcaagcgt cagtttggga aagatttcac gaccgatatt tctatggttg
     5161 ggggcttcgc ggcaacgtac gcgaggcgcg aactcgccat aacccaaaat tcaagtatga
     5221 ataccttgtg cgggttgcca gcatgttcac ctgtatctcc ggtacatcca tctctttcac
     5281 cgttcgcctg accgtccgca ccttccttcg cgaacacaac gacaatcccg gcttgagcga
     5341 taactcccaa gcgcgtctca cgttttaagg ccttttagcg cagccctcca tggccaccac
     5401 attccttttt accctggccg catttatcgt tgccctcggg ttgctgatcc tgtttcacga
     5461 gttcggtcat tacctggttg ctcgctggag cggagtaaag gtgcttcgtt tttcgatcgg
     5521 tttcggcaag cccttatata caaggcgaat cggagacgac cagacggaat gggtcatatc
     5581 tgcagttcct ctcggcggat atgtaaagat gctggatgag catgaaggcc acgtggagcc
     5641 ccaggatctg ccgcgtgcat ttaaccgcaa gcccgtcgct taccggttcg cgattgtcgc
     5701 agctgggcct cttgccaatt tcctgctggc aattcttctt tattgggcgc tgttcatact
     5761 gggtgtcacc gctctgaagc cggttctggg gccggtgcag ccgtccactc ccgcagcatc
     5821 tgcgggtttt cacgcgggcg aaaccattcg gactgttgat gctgaaccag tctcggcatg
     5881 gcaggatgtt cgctggctgc tgctctccaa ggttgcgggc acatcttcct ccgtcactgt
     5941 cgaaactcag gatgcggacg gcagacgcgc cgcacgaagc ctggatctga gtcagctgac
     6001 cgccgatgac ctcgaaggcg atttcctgaa gaaggtgggc ctggttcctt accagcctgc
     6061 gataaagccc atcatagcaa gcgtgtcctc cggtagcccc ggcgaacgcg ccgggttgca
     6121 gcccggtgac gaaattgttg cagtggatgg caaaaaggtc actttttggg aggacctggt
     6181 gcaagacatc cgtgaaagcc ctggtcgggc attgtcgctg gagatcctgc gtaataacag
     6241 aacggttgac ctggacgttg tacccgatac cgtgagtgaa ggcggcaaaa aaacaggaaa
     6301 acttggaatt gctccaaagg ttgatcacgc cgaactggaa aaactgttga ctgaggtcaa
     6361 gtattcgccg ggtgaagcta tcgtgaaggc ggtgagcaag acctgggaga tgtcgctgtt
     6421 cacgttgcga atgataggca agatggtggc cggggaaatg tcgtggaaga atgtcagcgg
     6481 ccccatcacg attgcagact atgcgggcaa gtccgcacag atgggaatta cgtcatacct
     6541 ggggtttctt gcggttatca gcatcagtct tggcgtactg aacttgttgc ccattcccgt
     6601 actggacgga gggcatttaa tgtattatgt gctcgaaatg atcaaaggca ggcctctttc
     6661 tgccagagcc atgcaagtcg gccagcaggt cgggatggcg ctcttgtttg ccctgatgat
     6721 ttttgcaata tataacgata tcagtcggct tatcccaact tgagtatgaa agtcaggacg
     6781 cttgttgcgc tcgtttctgc cttttgcgcc tcaatttcat gggcccgtga accttttgta
     6841 gtcaaggaca ttcgggtcga ggggatacaa cgtaccgaag caggcactgt tttcagctac
     6901 cttcccgtga aggttgggga tacgctcgat gacgaaaagg cggcggctgc aatcaaggcg
     6961 ctttatgcaa ccggcttctt caaggacgtg cggctcgagt ctgaaaacgg cgtgctgctc
     7021 gtggtggttg aagagcgtcc ggctatcgcc cagatcaccg ttgtgggatc gaaggaattc
     7081 gaaaaggaca agctgaaaga aggtttgaag caggccggcc tggccgaatc caggattttt
     7141 gaccgctcaa tgctcgaaaa ggccgagcag gaactgaagc atcagtatat cacccgaggc
     7201 aagtacggcg taaagatcac gacgaccacc actccactcg aacgcaatcg tgttgcgatc
     7261 aatttccata tcgacgaggg taaaaccgcg aagatacgcg ggataaatat catcgggaat
     7321 aaatccttca aggagcgtga cctgctgagc gcttttgcgc tgaggacgcc gggtctgttg
     7381 acctggttta cgaaggagga tcagtattcc aagcagaagc tgtccggtga cctggaggct
     7441 ttgaggtctt actatctcaa ccgcgggtac ctcgagttca gcatcgagtc tacccaggtg
     7501 tcgatcaccc ccgatatgca ggatatttac attacggtaa atatcacgga gggtccaaaa
     7561 tattccgttt ccgaaatcaa gcttgccggc gagatgcttg tcccggaagc cgacttgcgc
     7621 aagctcatca aaatgaaggt tggcgacgtg ttctcgcgcg agcacctgac cgagtctgtc
     7681 aagctcatca cggacaggct gggtgatgat ggctatgcat ttgccaacgt gaacgcatcc
     7741 ccccagctcg acaaggataa gcaacaggtc gcatttacct tttttattga tcctgggcgc
     7801 agggtttacg tcaggcgcat caatatcacg ggcaacagcc gtactcgaga cgaagtgata
     7861 cgccgcgaat tgcgccagtt tgaaagctcg tggtattcga caagcaagat caatcgctcg
     7921 aagcaacggg tcgacagact gaattatttc aatgccgtga gtatcgaaac ccctgcggtt
     7981 cccggcacga ccgatcagat tgatgtaaac gtggaggtca aggaaaagcc gactggcgcg
     8041 gtcatgttcg gagcgggcta ttcgaacatg gaagggctga tactgagcgg gtccattgcc
     8101 caggacaata tattcgggac gggcaagttc gttaacctaa tggtgaacac ggggagcgtg
     8161 aacaagactt attccctctc ctataccgat ccttacttca ccaaggatgg cgtaacggcg
     8221 gggttggatc tctacaagag gaccttgaat acaagcaccc tcgttgctgt gaacagcttc
     8281 aacaccgata cgactggggc gggcttgcgc tttggcatac ccatcaacga gaatgacagc
     8341 atcatggctg gcctcggggt ggaaaatgtc atcattcacc tgggggacaa cagtccgcag
     8401 cgtaacatcg atttcgtcca gagattcggg aaaagcacgc tcaatatacc gctgaccctt
     8461 aactggcggc gtgatggccg tgacagcgcc atatggacga cttcggggac cactcagcgc
     8521 gtcgctctcg agtctggttt gccgggaggt gacctgacgt actacaaggt aagctatgac
     8581 ctgaggtggt attatcctct gacagaaacg ttcacgctga tgctaaacgg tgaacttgga
     8641 tacggggccg gatataatgg taaagaactc ccctttttca agaatttctt tgccggcggc
     8701 aacacatcgg tgcgcggcta caatatcagc tccctgggcc ccagggacgt caataaccgc
     8761 acgctgggcg gtaataggcg tgcagtcggg aacgcagaaa tactgtttcc catgccaggc
     8821 atgagaaacg accggtctgt tcgcctgagc gcctttttcg atgctggatc ggtatggggc
     8881 ccggatggcg tcgtacctcg gcaggaggga ctgcgttatt ccgcaggtat tgcagttcag
     8941 tggatttcac ccatggggcc gcttaaaatc agtattgcgc aacctctgaa cagtcagcca
     9001 ggcgataata ttcaacgctt tcaatttcag ttcgggcaac agttctagtt ctcattggaa
     9061 catcgcctac ccagcccaat acacgcttgt tcacaagtac ttatgaataa tccggagata
     9121 aagtgggagc agcctgttcg ggtgccgtta ttcaagacgg gactcgttgt cttcgcattc
     9181 atctgggggg cgggtgccgc gacagcggat gagatcaaga tcggcgtggt ggatactgaa
     9241 agaatcctgg ctgagtccgc tccggcggtg agggcgctca agaaaataga aaaggaattc
     9301 ttgccccgcg accaggaact tggaaagatg gcctcccagg ccaaggcgct tcaggaaacc
     9361 cttgaaaaag acggaaaaac catgccggaa gcggaccggc gaagcaagga acgcgagctt
     9421 gccaacctca accgggagta ccaacgggct caacggcaga tgcgggaaga tcttagcctg
     9481 cgtcagaacg atgaagtcgc ggcgttgcaa ttgacggcta ccaaggcaat ccaggttatt
     9541 gccgaaaccg aaaagtacga tctgatacta cccttgcgcg accttgtcta tcgcagtcaa
     9601 cgcgtcgata ttaccgaaaa ggtcctcaag gctctggctg acaaataatt ttttactcaa
     9661 gcaaccccaa ggtctcccaa agtccctatt ttttccattc acatcgtcca atttcgattt
     9721 caatctgtta cctcatgctc gacgccaaaa actccggcca cgtgctcatg gacatccatg
     9781 aaatactcag atacctgccc caccgctatc cattcctgct ggtggatcgg gtgttgagtt
     9841 gtgagccagg aaaagatatc gctgctctga aaaatgtaac aatgaacgag ccattcttta
     9901 acgggcattt tccaggttat cccgtgatgc ccggggtttt gatcatcgaa gccctggcgc
     9961 aggctgccgg catactcacc ctcaggagca ccagcgtcaa accgaatgag gattcgcttt
    10021 tttacttcgt tggcatcgac gcggcgcgct tcaagaagcc tgtccagcct ggagaccagt
    10081 tgatcctcaa ggcaacgata ttacgggaac gaatcggcat atggaaatat tcggctcgag
    10141 cggaagtgga tgggcgtgtt gcagccgagg cagaattgat gtgcactgtc cggaataaac
    10201 agtagccagt gtccgtgtat tgattacggc ggcggagaaa gcatctgtgc cagccgcaat
    10261 catatatcgc cgtgcgggtg aacggagaag ggcacgccgg gtaaaactgc ttttgaagca
    10321 ggacaataaa ggggagtaca agagtgggcg ccgatgactt catcagggtc tgcggtgttg
    10381 atgaatctgg ccgcggtcca ctggcgggac ccgtatttgc cgcctgtgtc gtactcaatc
    10441 ctgcacgtcc aatcgaaggg ttggcagatt caaaaacgct ttccgaacag gcgcgcgaca
    10501 ggctgactgt cctcatcaag gagaattccc tcgcatgggc cataagctct gcatcagtcg
    10561 gggaaattga tcgcctgaac attcttcagg cgagcctcct cgccatgcgg cgcgcggtaa
    10621 aatcgctttc actcgtgccc gggagggttc ttgttgatgg gaatcaccga ccgcgcctca
    10681 gttatccggt aactgcaatc gtgaggggag acagtctggt accggaaatt tcggcagctt
    10741 caattattgc gaagacagcc cgtgatgcgg aaatggtggc actgcaccgg cgctttccgc
    10801 attacggctt tgaccggcat aaggggtatt caacagagca gcacattgaa gcgttgcgga
    10861 ttcatggtgc ctcggtcgtg catcggcgta gtttcgctcc tgtaagagaa ttgataagcg
    10921 gcgcctggaa gcatgagaac aaggaagcga gatagctggc gctccgcgtc accgaaataa
    10981 agctcactac ctgtactttc gcctattatt caattgtatt gcttgcttaa aacctgactg
    11041 aggttaacta tgcgtattga aaaagacacc gtggtatcgc tggattacaa gctctacgat
    11101 atgatgggaa aggtggtgga agagtccggc gagcccgtga gctatctcca tggaggctat
    11161 gacgccatat ttcctgccgt ggaagagcaa ctgaacgaca aggaagtggg ttacagttgc
    11221 tcggttctga tggagccgga agactctttt ggcgaatacg acagcgagct cgtgcgggtg
    11281 gaatcgcgcg aactgtttcc ggacaatatt gccgttggca tgcagttcga gggaacgcag
    11341 gaaggctcgg acgatacact catctatacc gtaacggaca tcgctgagga taaggtcgtg
    11401 gtggatggta atcatccgct ggctggcgtc gggctgcgct ttgattgcac tgtcactgct
    11461 gtgcggccag cgacgccgga tgaggtttct catggtcatg cccatgggcc cgacggacat
    11521 ggacacgacc actaaaaact aaccgcctgc cttcgcgcag tcggcgtaac ctgttattgt
    11581 tcgactgttt ttttgagcgc ataaagttct tcaagcgctt gacgtgcact cagttcatcg
    11641 atatccagcg cgcgcaa
//
LOCUS       DASSHJ010000028         5192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1402190,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000028 DASSHJ010000000
VERSION     DASSHJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402190"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFI43_01385"
     CDS             complement(<1..616)
                     /locus_tag="VFI43_01385"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET7060813.1"
                     /translation="MPYSENKGSPSSKGAADLIRTILIMAGGTGGHVFPGLAVAEYMK
                     KAGWHVVWLGTEGGMETLLAPQQGFEIETIRFSGFRRKGAGAWAMLPLRLSLALWQSA
                     KVMLRVRPDVVLGMGGYPSFPGGLMASLLDKPLLIHEQNSIPGLVNRILTKLADRVLL
                     GFPVAKMKDQHTLFSGNPVRAEILQVEAPEKRYGSRSGKLRLLVI"
     gene            complement(606..1769)
                     /gene="ftsW"
                     /locus_tag="VFI43_01390"
     CDS             complement(606..1769)
                     /gene="ftsW"
                     /locus_tag="VFI43_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET7060814.1"
                     /translation="MIYQQDIGKSRNLPDFDHALIWSAIFLLSLGLVMVYSASISTAE
                     AGRGVDGFPAHFLVRHGGYVAVGLLAGVIAFQVPTRVWQKYSFHLFLLGLVLLVLVLI
                     PGIGREVNGSQRWISLLVVNFQPSEFMKLFMVIYVSSYTVRKAVYLDDFRKGFLPMLM
                     VMLTVGLLLLLEPDFGSFVVITAIVMSILFLGGINLKLFTGLIGFVVTGFLALIWAEP
                     YRLQRFFGFMNPWDDPYGKGYQLSHALIAFGRGEWFGVGLGGSVEKLFYLPEAHTDFL
                     LAVIAEELGFAGVATTVILFACLVFRAFGIGRQAARRESFFAALLAQGIGVWLGVQAF
                     INMGVNMGILPTKGLTLPLMSFGGSSIVASCLSLALLLRVDWENRQAIKGYAV"
     gene            complement(1766..3202)
                     /gene="murD"
                     /locus_tag="VFI43_01395"
     CDS             complement(1766..3202)
                     /gene="murD"
                     /locus_tag="VFI43_01395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7060815.1"
                     /translation="MNLCGTNVLVLGMGETGLSMIRWLLRAGAHVRAVDSRTAPPCLD
                     AIGKILPADSIYTGGFAAEAFVNIGLITISPGVAVSEPAVRNAASRGIPFMGDVELFA
                     LAIEELPFSKPKIVAITGSNGKTTVTAMVGSMLKNAGWDAEVAGNIGPAVLDVLMERL
                     DSGKLPQAWVLELSSFQLETTHNLCPDAAAVLNLSEDHFDRYAGMQDYARAKARIFQG
                     DADPGMRTSGGVQVINRDDMLVRAMALPGRKRMTFGLDLPAGADDFGLSRDGKRVWLM
                     HGDLRLMEASELLVGGLHNVANALAALALCRALALPFEPLLQALREFKGLPHRMERVA
                     SFGGVCFYDDSKGTNVGATVAALNGAAETVVLIAGGDGKGQDFSPLVRPVAEHARAVV
                     LIGRDAGRISEVLRHCEKPLHFAETMEEAVRSSFLLANEGDAILLSPACASFDMFKNY
                     VHRAQSFVAAVNRLQTENSAVVPVSEAQ"
     gene            complement(3211..4296)
                     /gene="mraY"
                     /locus_tag="VFI43_01400"
     CDS             complement(3211..4296)
                     /gene="mraY"
                     /locus_tag="VFI43_01400"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7060816.1"
                     /translation="MLLELAQWLAKDIRVFNVFHYITLRTVLAALTALMISFIVGPAM
                     IRKLTAYKIGQSVRDDGPQTHLVKAGTPTMGGALILVSMAITTLLWADLSNRYIWVAL
                     VTTLGFGIVGWVDDYRKVVYRNPKGLSARAKLLWQSVIAISVALYLGLSAELPAQTTM
                     IVPFFKQVAIPLGLAGFVALAYFVIVGTSNAVNLTDGLDGLAIMPTVMISSALAIFAY
                     VAGHAVFSKYLGIPHIPQAAELAVFCGALAGAGLAFLWFNAYPAEVFMGDVGALGLGA
                     ALGVVTVIVRQEIVLFIMGGVFVVETLSVMLQVASFKLIGKRVFRMAPLHHHFELKGW
                     KENQVVVRFWIITMMLVLFGLSTLKLR"
     gene            complement(4333..>5192)
                     /gene="murF"
                     /locus_tag="VFI43_01405"
     CDS             complement(4333..>5192)
                     /gene="murF"
                     /locus_tag="VFI43_01405"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7060817.1"
                     /translation="MNHACEISYLTRLAKPHIAVITNAGVAHLLGLGSVEAVARAKGE
                     IFEGLDSSGIAVINADDEHAPLWRELADGRSRLEFGLQGTADVSARYELGSFGARVTL
                     ILPDGTQETELRVPGVHNIRNALAAAAVASAMGIGKAAIALGLNQFGGVNGRMQKKYG
                     LHGATLIDDTYNANPESVRVAIDVLASAGGTTVLVLGDMGELGDSAADLHERIGTESR
                     EAGIDKLFALGELSRHSAASFGPGARHYETMEELIPDVLELLAPDVTLLVKGSRFMKM
                     ERLVKSIEL"
BASE COUNT         1235 a         1635 c         1327 g          995 t
ORIGIN      
        1 cgatcacgag caacctcagc tttccgctgc gtgaaccgta tcgcttctcc ggcgcctcca
       61 cctgcaggat ctcggctcgg acggggttgc cggaaaacaa cgtatgctgg tctttcattt
      121 tcgcgactgg aaaacccagc aacaccctgt cggccagctt cgtcaatatc cgatttacga
      181 ggcctggaat ggaattctgc tcatgtatca gcagtggctt gtccaacaat gaagccatca
      241 atccgccggg aaacgatggg taaccgccca ttcccagcac aacatccggg cgaacccgga
      301 gcatgacctt cgcgctctgc cataaagcca gcgaaagccg gagagggagc atggcccatg
      361 ccccagcccc tttccgccgg aagcccgaaa agcggatggt ctcgatctcg aatccttgct
      421 gaggtgcaag caacgtttcc atgcctccct cggtcccgag ccagacaacg tgccagcctg
      481 ccttcttcat gtattccgcg acggcgagcc caggaaacac atgaccgccc gtcccccccg
      541 ccatgatgag aatcgtacgg atcaagtccg ctgcgccctt tgacgagggc gaccctttgt
      601 tttcgctata cggcataacc tttgatcgcc tgccggtttt cccaatcgac gcgtaacaac
      661 aaggccagcg aaaggcaact ggcgacaata ctgctgccac cgaaactcat cagcggaagc
      721 gtcaatcctt ttgtgggcag aatgcccatg tttacgccca tgtttatgaa tgcctgtacc
      781 ccgagccata cgccgatccc ctgcgcgagc aacgccgcga agaaactttc acgtctcgcg
      841 gcctggcgtc cgatcccgaa agcgcgaaac accaggcatg caaacagtat gaccgtcgtc
      901 gcgacaccgg cgaagccaag ctcctccgca atgactgcca gcagaaaatc ggtgtgcgct
      961 tccggcagat aaaagagttt ctccacgcta ccgcccaacc ccaccccgaa ccattcgcca
     1021 cgtccaaaag caatcagcgc gtggctcagt tgatatcctt tcccatacgg gtcatcccat
     1081 gggttcatga atccaaagaa gcgttgcagg cgataaggct cagcccagat gagcgcgaga
     1141 aaaccggtca ccacaaagcc gatcagccct gtgaatagct tgagatttat tccacccaga
     1201 aacaggatcg acatcacgat cgctgtaatc acgacaaacg acccgaagtc cggctcgagc
     1261 aggagcagca aacctactgt gagcatgacc atcagcatgg gcaggaagcc cttgcgaaaa
     1321 tcatcgagat aaaccgcctt gcgaaccgta tagctggaaa cgtaaatcac catgaacaat
     1381 ttcatgaact cggagggctg gaaattgact accaggagcg atatccagcg ctgactgcca
     1441 ttgacttcgc gcccgatgcc agggatcaat accagcacca gtaacacgag accgagcagg
     1501 aaaaggtgaa aggaatattt ctgccatacc cgcgtaggca cctgaaatgc aatcacgccc
     1561 gctagcagcc caacggcaac atatccacca tgacgcacga gaaaatgagc aggaaagccg
     1621 tccacccccc gtcccgcctc ggcagtggaa atggaagcgg aataaaccat caccaacccg
     1681 agactcagca ggaatattgc ggaccagatc aacgcatgat cgaaatcggg gagatttcgg
     1741 gatttaccga tgtcctgctg ataaatcatt gggcttcaga taccggaaca acggcactgt
     1801 tttctgtctg aagacgattt actgccgcga cgaatgactg ggcgcgatga acgtagtttt
     1861 tgaacatgtc gaagctcgcg catgccggcg acaacaatat cgcatcacct tcattggcaa
     1921 gcaaaaagct gctacgtacg gcttcttcca tcgtttccgc gaaatgcagg ggcttctcgc
     1981 aatggcgcag tacctcggag atcctgcctg cgtcgcgacc gatcagcacg acggcgcgcg
     2041 cgtgctcagc gacgggtctc acgaggggag agaaatcctg gcccttgcca tcgccacccg
     2101 caatcagcac gactgtttct gccgccccgt tcagcgccgc aaccgttgcc cctacattgg
     2161 tgccttttga atcgtcgtag aagcagacgc ctccaaacga cgccacgcgc tccatccggt
     2221 gcggcaggcc cttgaattca cgcagagctt gcaataaggg ctcaaatggc aatgccaggg
     2281 cgcggcatag cgccagggca gcgagcgcat tcgccacgtt gtgcaaacca cccaccagaa
     2341 gctcgctcgc ttccatcagc cgcaggtctc cgtgcatcaa ccagacgcgc ttgccatcac
     2401 gcgacaaacc aaaatcgtcc gccccggccg gcagatccaa accgaacgtc attcgcttcc
     2461 ggcccggcaa tgccattgcg cgcaccagca tgtcgtcgcg attgatgact tgcaccccgc
     2521 cgctcgtgcg catgccggga tctgcgtccc cttggaatat tcgggctttg gcgcgggcat
     2581 aatcctgcat tcccgcataa cggtcgaaat gatcctcgct taaattgagc accgctgccg
     2641 cgtcagggca aagattatgc gtggtttcca gctggaagct ggaaagctcc agaacccacg
     2701 cctggggcaa tttcccggag tccagccgtt ccatgagcac atccagcacg gctgggccga
     2761 tattgcctgc tacctccgcg tcccatccgg cattcttcaa cattgatccc accatggcgg
     2821 tcaccgtcgt ttttccattt gaccctgtga tcgccactat ttttggcttt gaaaaaggaa
     2881 gttcttcgat cgccaaggcg aaaagttcca cgtcacccat aaagggtatg cccctgctgg
     2941 ccgcgttccg aacggcaggt tccgacaccg ccactcccgg gctgatcgtg atcagcccaa
     3001 tatttacaaa ggcctcggct gcaaatcctc cggtgtatat cgaatcggca gggaggattt
     3061 ttcctattgc atccaggcag ggtggagcgg tgcggctatc aaccgcgcgc acgtgggctc
     3121 ccgcgcgcaa aagccacctg atcatggaaa gcccggtctc ccccattcca agcaccagca
     3181 cgtttgtccc gcaaaggttc atgaccaggg ctacctcagc ttcaacgtcg acaggccgaa
     3241 cagtaccagc atcatggtga tgatccagaa tcgaaccacc acctgatttt ccttccagcc
     3301 cttcaactcg aaatggtgat gcaggggcgc catgcgaaat acgcgctttc cgatcagctt
     3361 gaaggaagcg acctgcaaca tcacggacaa ggtctccacg acaaataccc ctcccatgat
     3421 gaacagcacg atttcctggc gaacaatcac cgtaacgaca ccgagcgcag cgccgagccc
     3481 aagcgcgcca acgtcgccca tgaaaacttc tgccggatag gcgttgaacc agagaaaggc
     3541 caggcccgcg cccgccagcg cgccgcagaa aaccgcgagt tctgctgcct ggggaatgtg
     3601 aggaatgccg agatacttcg aaaacacggc gtggcccgcc acataggcaa aaatggccag
     3661 cgcgctgctg atcatcactg tcggcatgat cgccagccca tcgaggccat cggtgagatt
     3721 caccgcattg ctcgtcccga caatcacgaa ataagccagc gccacgaacc cggccaaacc
     3781 cagcggaatg gccacctgct tgaaaaatgg aacgatcatg gttgtttgtg cgggtagttc
     3841 cgccgacaag cccagatata gcgcaaccga gatcgcgata acagattgcc agagcagctt
     3901 ggcgcgcgcc gagagccctt tgggattgcg atacacgacc ttgcggtaat catcgaccca
     3961 gccgacaatg ccgaatccga gggtagtcac aagcgcaacc catatataac ggttactcag
     4021 atccgcccac aacagggtgg tgatcgccat ggagaccagt atgagtgcgc cgcccatcgt
     4081 aggcgttccc gctttcacca ggtgcgtctg cggtccgtcg tcgcgcaccg actggcctat
     4141 cttgtatgca gtcagcttgc gaatcatcgc gggaccgact ataaaagaga tcatgagggc
     4201 tgtcagcgcc gccagtaccg tgcgtaaagt aatgtaatgg aatacgttga atactcgtat
     4261 gtcctttgca agccattggg cgagctctag cagcatgggc gtcccgaaga tgagactctg
     4321 tatttcatga ggtcacaatt cgatgctctt gacaagccgc tccatcttca tgaaacgcga
     4381 tccctttacc agcagggtta cgtcaggcgc aagcagctcc aacacatctg gaatcaactc
     4441 ctccattgtc tcgtagtggc gggcgccggg tccgaaagaa gccgcgctgt ggcggctcaa
     4501 ttctccaagg gcgaacagct tgtcgattcc ggcctcccgg ctctctgttc cgatacgctc
     4561 gtggagatcg gctgcagaat cgcccagctc gcccatgtca cccaaaacca gaaccgtcgt
     4621 tcccccagcg ctcgccagca cgtcaatcgc cacgcggacg gattccgggt tggcgttgta
     4681 cgtgtcatcg atcaaggtgg cgccatgcag cccgtatttt ttctgcatcc ggccattcac
     4741 gccgccaaat tggttcaagc caagcgcaat cgcggctttg cctatgccca tggccgaggc
     4801 aaccgctgcg gcagccaatg cattgcggat attatgaacg cccggtacac gcaactcggt
     4861 ttcctgcgtc ccgtcgggca gaatcaaggt tacgcgggct ccaaacgaac cgagctcata
     4921 acgagcgctg acatcggcag tgccttgcaa accgaactcc agccggctcc tgccgtccgc
     4981 aagttcccgc catagcggag catgttcgtc atcggcattt atcacggcga ttccggatga
     5041 atcgagacct tcaaaaattt cccccttcgc gcgcgctacg gcttccacgg acccaagccc
     5101 cagaagatgc gctacgccgg cattggtaat cacagctata tggggtttcg ctaacctcgt
     5161 caggtaagag atttcgcaag catgattcat gc
//
LOCUS       DASSHJ010000029         8379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_527844, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000029 DASSHJ010000000
VERSION     DASSHJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527844"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..797)
                     /gene="rplC"
                     /locus_tag="VFI43_01410"
     CDS             complement(81..797)
                     /gene="rplC"
                     /locus_tag="VFI43_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7060818.1"
                     /translation="MSLGLVGRKVGMTRIFTGEGDSLPVTVLDVSNNRVTQIKTPGTD
                     GYSAIQVTYGKRRPVRVNKPSAGHFAKAGVEAGQVLKEFRVTSEVVENFKPGAVIGLD
                     LFKVGQRVDVTGTSIGKGYSGVIKRHGFSSNRASHGNSKSHNVPGSIGMAQDPGRVFP
                     GKRMSGHLGNVQRTIQNLEILRVDNDRGLLVLKGAIPGSKGGDVVIRPSVKVTKVRVA
                     PVSKGDVKPAGKSGTKSGKK"
     gene            complement(919..1227)
                     /gene="rpsJ"
                     /locus_tag="VFI43_01415"
     CDS             complement(919..1227)
                     /gene="rpsJ"
                     /locus_tag="VFI43_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7060819.1"
                     /translation="MQSQKIRIRLKAFDYRLIDKSAMEIVETAKRTGAVVKGPVPLPT
                     RIERFDVLRSPHVNKTSRDQFEIRTHLRLMDIMDPTDKTVDALMKLDLPAGVDVEIKL
                     "
     gene            complement(1316..2506)
                     /gene="tuf"
                     /locus_tag="VFI43_01420"
     CDS             complement(1316..2506)
                     /gene="tuf"
                     /locus_tag="VFI43_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7060820.1"
                     /translation="MAKSKFERKKPHVNVGTIGHVDHGKTTLTAAITMVLAKKFGGEA
                     KSYAQIDSAPEEKARGITINTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYMNKADMVDDAELLELVEMEVRELLSK
                     YNFPGDDTPIVIGSALKALEGDQSDIGEPSIYKLADALDSYIPEPVRAVDGAFLMPVE
                     DVFSISGRGTVVTGRIERGVIKVGEEIEIVGLRPTIKTVCTGVEMFRKLLDQGQAGDN
                     VGVLLRGTKREEVERGQVLAKPGTITPHTKFSAEIYVLSKEEGGRHTPFFQGYRPQFY
                     FRTTDVTGAIELPAGVEMVMPGDNVSVTVNLIAPIAMEEGLRFAIREGGRTVGAGVVA
                     KIIE"
     gene            complement(2557..2631)
                     /locus_tag="VFI43_01425"
     tRNA            complement(2557..2631)
                     /locus_tag="VFI43_01425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2597..2599),aa:Thr,seq:ggt)
     gene            complement(2740..2813)
                     /locus_tag="VFI43_01430"
     tRNA            complement(2740..2813)
                     /locus_tag="VFI43_01430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2779..2781),aa:Gly,seq:tcc)
     gene            complement(2934..3018)
                     /locus_tag="VFI43_01435"
     tRNA            complement(2934..3018)
                     /locus_tag="VFI43_01435"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2982..2984),aa:Tyr,seq:gta)
     gene            complement(3329..3793)
                     /locus_tag="VFI43_01440"
     CDS             complement(3329..3793)
                     /locus_tag="VFI43_01440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7060821.1"
                     /translation="MSRVNIRVKSALAILGIALLPFAAFSQADKGTANVTELIKKDVK
                     VGTGEEAVIGKMAEVQYTGWLYDPAAPDKKGKKFDSSRDRGTPFSFLLGAGRVIKGWD
                     RGVTGMKVGGERILIIPPAMAYGSQGAGNVIPPDATLIFDVELVGLRQGSTH"
     gene            3970..4422
                     /locus_tag="VFI43_01445"
     CDS             3970..4422
                     /locus_tag="VFI43_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET7060822.1"
                     /translation="MSPSRIHDSRLVRTLYLVAGFTSLILAVVGIVLPILPTTPFVLL
                     AAACFARSSKRFHDMLLANRVAGPIIHEWTEHRSVPRRVKRWVYFLLALSFGSSILVM
                     PSPWYQGMLALLGAVLAVFVWRIPVRETAQPSDRITETGMGKVSDENR"
     gene            complement(4472..5164)
                     /locus_tag="VFI43_01450"
     CDS             complement(4472..5164)
                     /locus_tag="VFI43_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7060823.1"
                     /translation="MHNQLAVISLATVLILSGCGSTPQLSNPTQDTATGAGVGTGAGA
                     LISAIAGGGKGADIGGAPGAVPAAPAASVGNVWSRRMEEQKKAMEQATAGTGVQVVKT
                     ADNRLKLDIPSDISFDAGSAKIKPDFRPVLDNLATSVVNNPGAEISIIGHTDSSGSDV
                     ANNPLSLNRAASTGDYLVSRGVSATRITIDGHGWRRPLVSDDTPANRAKNRCVEIFVA
                     EPQAPSQRISNK"
     gene            complement(5306..6211)
                     /gene="xerD"
                     /locus_tag="VFI43_01455"
     CDS             complement(5306..6211)
                     /gene="xerD"
                     /locus_tag="VFI43_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7060824.1"
                     /translation="MAESDNALLDEFLDTLWLEDGLSRNTLESYRSDLRQFGGWLATL
                     RTYDNTLVSAVRSDLLAFLAQKVSARVKASTTSRQLSSLKRFYRYLFQQGRIKVDPSL
                     NIDSPKLSRNLPASLTEEDVEALLNAPDVKQSMGVRDRTMLEVLYASGLRVSELVSLE
                     PGQINLSMGIVRVFGKGGKERLTPLGEEALEWVVRYTKEARLDLLNGKLSAALFPTSR
                     GSAMTRQAFWYLIKRYARQAGITKHLSPHTLRHAFATHLLNHGADLRVVQLLLGHADI
                     STTQIYTHVARERLKRIHEIHHPRG"
     gene            complement(6198..6704)
                     /locus_tag="VFI43_01460"
     CDS             complement(6198..6704)
                     /locus_tag="VFI43_01460"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7060825.1"
                     /translation="MSNSKTDPGVSPPEYQAKLATPFAVLGIRIEEDWLTDIEYLPPD
                     TDTLPPQTSLAAQVCEQLQAYVADPTFTFDLSLHIGGTNHQRRVWRAIQDIPSGRTRS
                     YAEIANELHSAPRAVGQACGANRLPIVIPCHRVITKSGGLGGFMNSSGGLPLDIKRWL
                     LCHERGGI"
     gene            6935..>8379
                     /locus_tag="VFI43_01465"
     CDS             6935..>8379
                     /locus_tag="VFI43_01465"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7060826.1"
                     /translation="MKFSIKKVNPEKQRAACVVVGVFEPRKLTEAALALDRAAGGSIS
                     NILNSGDMDGKAGTARLLHNVPNTLCERVLLVGLGKEEELSEKRYRDAIRAAFRALND
                     TGAADATFFLTGIPVRNRDSAWKVSHTAIVAMESSYRFDRLKSKVDDKPPHLRKITLG
                     ITTGEASEVPAAESALQQGIAIAEGMHLAKDLGNLAPNICTPTYLAEQAAGLAKTYKL
                     KATILEQKDMEKLGMGALLAVARGSRQPAKLIVLEYWGRSKKEKPVVLVGKGITFDTG
                     GISLKPAPEMDEMKYDMCGAASVLGAINAIAKMRLPLNVVGIIPTTENMPGGNATKPG
                     DVVTSMSGQTVEILNTDAEGRLILCDALTYAERFEPDTVVDIATLTGACVIALGHVAS
                     GLLTPDDDLAEELLHASEQAADPAWRLPLWDDYQEQLKSNFADMANIGGRAAGTITAA
                     CFLWRFTKKYRWAHLDIAGTAWKSGKEKGSTG"
BASE COUNT         1910 a         2413 c         2170 g         1886 t
ORIGIN      
        1 ttcacgggcg aacaacgcat cggtgccaga aatgctggcg gatgactctc cattatcgtc
       61 tatgagcttg agttccatgc ctacttcttc cctgatttcg taccactctt gccggccggc
      121 ttcacatcac ccttgctaac cggcgcgacc cggaccttgg taactttcac gctcggacgg
      181 attaccacat cgccgccttt tgatcccgga attgcaccct tgaggaccag caacccgcga
      241 tcattgtcca cacgcaagat ctcaagattc tggatcgtac gctgaacatt tcccaagtgc
      301 cccgacatcc gcttcccggg aaacacacgg ccggggtcct gcgccatgcc gattgaacct
      361 ggaacgttat gggatttgga attgccgtga cttgcacgat ttgaggaaaa accgtgccgc
      421 ttgatgacac cactgtaacc cttgccgatc gaagtacccg tcacatccac ccgctggcct
      481 accttgaaaa ggtcaaggcc gatcacggcg ccgggcttga aattttcgac cacctcggac
      541 gtgacgcgaa attccttgag tacctgtcct gcttcaactc cagctttggc aaaatgtccg
      601 gccgaaggct tgttgacgcg caccggccga cgctttccat aagtgacctg aatagcggaa
      661 taaccatctg tgcccggcgt cttgatttgc gtcacccggt tgttcgacac gtcgagcacg
      721 gtcacgggca ggctgtcacc ttcacccgta aaaatacgag tcatgccaac cttacggccg
      781 acaagtccta aactcatttt aattaccctt cctgaaaaag gggccgatta caattgatcg
      841 gccgtaacaa taatagcgtt ttggatcagc tacccaggca gtagcctgat atagctatag
      901 aaggtccgaa ttcaaagcct ataacttaat ctcgacatcc actcctgcag gcaggtctaa
      961 cttcatcaag gcgtccaccg tcttgtcagt ggggtccatg atgtccatca ggcgaagatg
     1021 agttcgaatt tcgaattgat ctcgtgaagt cttgttgaca tgaggagaac ggagtacgtc
     1081 aaagcgttcg atacgcgttg gcaaaggaac ggggcctttc actacagcac cggttcgctt
     1141 cgcggtttca acgatttcca tcgccgactt gtctattaac cggtaatcaa atgctttcag
     1201 acgaatgcgg attttttggc tttgcataaa tcaaacctca ggttgaggat ccggattgcg
     1261 aattgggaat ggatgtcccg catccactcc tgaatccgat ccttggccct tgttgttact
     1321 cgataatctt tgcgacgacg cctgcgccga cggttcttcc gccttcgcgg atggcaaagc
     1381 gcagaccctc ttccatcgca atgggtgcaa tcaggttgac cgtcaccgat acgttgtccc
     1441 caggcattac catctcaacc ccggcaggca gttcaatcgc gcccgtcacg tccgtggtgc
     1501 gaaagtaaaa ctgcggacga tagccctgga aaaaaggggt gtgccttccg ccttcttcct
     1561 tgctcagaac gtatatctcg gcagaaaact tggtgtgcgg cgtgatcgtg cctggcttgg
     1621 ccagcacctg cccacgctcg acttcttcac gcttggtgcc gcgaagcaga acacctacat
     1681 tgtctccagc ctgcccctga tccagaagct tgcgaaacat ctccacaccg gtgcataccg
     1741 tcttgatcgt cggacgaagt cctacaatct cgatttcttc cccaaccttg atcaccccgc
     1801 gttctatccg cccggtcact accgtgccac gcccggatat cgaaaatacg tcttcaacag
     1861 gcatcagaaa ggcaccatcc accgctcgca ccggctccgg tatgtagctg tccagtgcgt
     1921 ccgcaagctt gtagatcgag ggctccccga tgtcgctctg gtctccctcc agcgccttca
     1981 gggcagaccc gatcacgatc ggggtgtcgt ccccgggaaa attgtatttc gacaaaagct
     2041 cgcgaacttc catctcgacc agttccagaa gctcggcatc gtccaccatg tcggccttgt
     2101 tcatgtacac gacaatgtag ggtaccccta cctggcgcgc cagcaggatg tgctcgcgcg
     2161 tctgcggcat cgggccgtcc gcagccgata ccacaagaat cgccccgtcc atctgcgccg
     2221 cacccgtgat catgttcttc acgtagtcag cgtgcccagg acagtccacg tgcgcgtaat
     2281 gacgcttctc cgtctcgtat tccacatgcg ccgtgttgat cgtgatcccg cgagccttct
     2341 cttccggcgc cgagtcaatc tgcgcgtagc tcttcgcctc tccaccaaac ttcttcgcca
     2401 ataccatcgt gatcgccgcc gtcaacgtgg tcttgccatg atccacgtga cctatcgtcc
     2461 caacattcac atgcggcttc ttccgctcaa atttgctctt tgccataatg aatcctctta
     2521 aagcacttat ttctcgccgg tttcaattaa agcctatgga gcccatggcc aggattgaac
     2581 tggcgacctc tcccttacca agggagtgct ctgccactga gctacatggg caaatccgtt
     2641 ccacctgaat cactttctca gcagcattgc gactcaacga aacaactctg caataacttt
     2701 atataaattt atataaatat ggctttgcgt aagtaaatat ggagcgggtg aagggaatcg
     2761 aaccctcgtc gtaagcttgg aaggcttctg ctctaccatt gagctacacc cgcgtatcaa
     2821 ccaatcgacc catcacccgg gcacgcccta ttcgcatgac tgaacttgcg caccttttaa
     2881 agcagtttaa cgcgggcttt aaagttgaca gcaaggattc gctcgcctcc agatggtgga
     2941 gggggaagga ttcgaacctt cgaaggcaga gccgtcagat ttacagtctg atccctttga
     3001 ccgctcggga acccctccaa acgaaactgt ctattgtgaa tgctttatcc cgataagtca
     3061 atgtggattt tgcttgactg ttgagcccat ggcagcacag aaaacagaag gataaataca
     3121 tttgagggaa cgcaccggat ggaggtctga gtaacttatt gatgggagag aaacttcaat
     3181 aaaaaccccg ttttttccat ctcccgcagc ccgcaggcac gaaccccaag ttccggcgca
     3241 ggacgacagg acgttgagtg cgaatcgatt cattcatttg tcgaaagttc cgattccgcc
     3301 gattcgaacc tcgttgtccc ttgaaaagtt aatgtgtaga accttgcctt agacccacca
     3361 actctacatc gaagatcagc gtagcgtcgg gaggaatgac attgcccgcg ccttgcgacc
     3421 cataagccat ggcaggcgga ataatcaaga tgcgctcgcc cccaactttc attcctgtca
     3481 caccgcgatc ccaaccttta attacgcgcc cggccccaag gagaaaagaa aaaggcgtgc
     3541 cgcgatcgcg cgaactatcg aatttcttgc ccttcttatc cggcgccgcg ggatcatata
     3601 accagccggt atattgcact tccgccatct tgccaatgac cgcctcttct ccagttccaa
     3661 cctttacatc cttttttata agttcggtca cgtttgccgt tcctttgtca gcttgggaaa
     3721 aagcggcgaa gggtaatagc gctataccca agattgccag ggccgatttc acccgtatgt
     3781 ttactctaga catgattacc tcaattaaac acagaaacaa cgacaagctc ccaaccccac
     3841 gttatttgag gggcaaggtg tgccacaatt cgacattcgg gtgcaaactc ccagaccgat
     3901 atcagttcga tgcacttctc gaggagcagg tatggcagga ttgaaaagaa tcagccaacg
     3961 atcgatacga tgagtccgtc gagaatacat gattcccggc tggtgcgcac cctatatctg
     4021 gtcgccggct tcacctccct catattggct gtggttggca ttgttctgcc aatattaccg
     4081 acaacgccct tcgtcctctt ggcagcagcg tgttttgcac gcagctcgaa gcgttttcat
     4141 gacatgctac ttgccaatcg cgttgccggt cccatcatcc atgaatggac cgagcaccgc
     4201 agcgtccctc gccgggtaaa acgctgggtc tacttcctct tggcattgtc cttcggcagc
     4261 tcgattctcg tcatgccgtc tccatggtat caggggatgc ttgcgttgct cggtgcagtc
     4321 cttgccgtgt ttgtctggcg gataccggta agagagacag cgcaaccatc agaccggatt
     4381 acggagaccg gaatgggtaa agtctccgac gagaatcggt aagcggtcag gacgagatca
     4441 tccactcaag ctttcgccta ttgcgtcttg ctcacttatt gcttatccgc tgagaaggag
     4501 cttggggttc cgccacgaag atttcgacgc accggttttt cgcgcggttt gcaggcgtgt
     4561 catccgaaac cagcggtcga cgccaaccgt gcccatcgat cgtgatccgg gtcgccgaaa
     4621 cgcctctgct gacgaggtaa tcgccagtgc tcgcggcccg attgagcgac aggggattat
     4681 tggcgacgtc gctaccggaa ctgtcagtgt ggccgataat gctgatctcg gcccctggat
     4741 tgttgacgac gctcgtggcg agattgtcca gcaccggcct gaaatcaggt ttaatcttcg
     4801 cactgccggc gtcgaaggaa atatcgctgg gaatatcgag cttcagccgg ttatccgcgg
     4861 tcttgacgac ctgtacccct gtgccagcgg ttgcttgttc cattgccttc ttttgctctt
     4921 ccatcctgcg tgaccaaacg ttgccaacac ttgctgccgg ggctgccgga actgcgccgg
     4981 gggctccgcc aatgtctgcg cccttcccgc ctcctgcaat agcgcttatc aatgcgcccg
     5041 cacccgtgcc aactcctgcc ccagtggcag tgtcctgggt cgggttactc aactggggag
     5101 tactaccgca accagacaat atcaaaacag tggcaagcga aatgaccgca agttgattat
     5161 gcatgatctc tcctcattta tcggtgattt cttatggcaa aacacgaact cttcctttaa
     5221 ttttaccaga agagaatgac gacgccagga gctcgcgtgc gcaggcaacg gatgcgagga
     5281 gagttgacgt aaaatgctgt tgccatcatc cgcgtggatg atgtatttca tgaatgcgct
     5341 tcaagcgttc tcgcgccaca tgcgtgtaga tctgggtggt ggaaatatcg gcatggccaa
     5401 gcagcagttg cacaacgcgc aggtcggcac catggttgag aaggtgggtg gcgaacgcgt
     5461 ggcgcagagt atgcggcgac aggtgcttgg taataccggc ctgcctggcg tagcgcttga
     5521 tcagatacca gaatgcctgg cgcgtcatgg ctgaaccgcg agaggtggga aacaaggccg
     5581 cgctcagttt cccgttcagc aagtccagcc gggcttcttt cgtataacgg accacccact
     5641 cgagcgcctc ctctcccaga ggagtgagac gctcttttcc gccttttccg aaaacccgca
     5701 caatccccat gctcaaattg atctgtcccg gttcaaggct aaccagctct gatactcgca
     5761 atccgctcgc atacagaacc tcgagcatgg ttcggtcgcg tactcccatc gactgcttta
     5821 cgtccggcgc attcagcagt gcctcaacgt cctcctcagt caagctcgct ggcaaattgc
     5881 gcgacagctt gggtgaatcg atattcaagc ttggatcgac cttgattctt ccctgttgaa
     5941 agagatagcg gtagaaccgc tttaagctgg aaagttggcg gctagtggtg ctcgccttga
     6001 ccctggctga gactttttgc gcaaggaacg ccagcaggtc cgaacggacg gcagaaacca
     6061 gcgtgttgtc atacgtcctg agggtggcga gccatccgcc gaactgccgc aagtcgctgc
     6121 gataactttc aagcgtgttc cgggataaac cgtcctccag ccagagggta tccagaaatt
     6181 catccagcag ggcgttatca gattccgcca cgctcgtgac acaaaagcca gcgcttgata
     6241 tcgagaggaa ggccgccgct cgaattcatg aaccccccga gcccgccgct ttttgtaatg
     6301 acacggtggc agggaataac aataggaaga cgattcgccc cgcacgcctg ccccaccgcc
     6361 cgcggagcgg aatgcaactc attggcgatt tcggcatagc tgcgtgttct accgctggga
     6421 atgtcctgga tggcccgcca tacccggcgc tggtgattgg ttccgccaat atgcagggag
     6481 aggtcgaaag taaaggtcgg gtcagctacg tatgcctgca gttgctcgca aacctgtgct
     6541 gcaagagacg tctgtggcgg aagcgtgtcc gtatcgggag gaagatactc gatatccgtc
     6601 agccagtctt cctcgatacg gatgcctaaa acagcaaacg gagtagcgag cttcgcctgg
     6661 tattcaggag gcgaaacccc tggatcggtc tttgaattgc tcatggaaac ggtctatgaa
     6721 ggatatgtcg tcaaccatat tttgacgtga taacagcggc aggccgtgca tgagcaggct
     6781 cttttcatgc tttactttgc ccaagcagac cactgataat cggttattat cccgcttgag
     6841 ccatttatgg gcaagcctcc gagcgaagcc ttccgcgaga aggtaaccgg caccggatca
     6901 tccctcagca agactttgaa ttaaggagca ccccgtgaag ttcagcataa aaaaagtaaa
     6961 tcccgagaag cagcgcgccg catgcgtagt cgtaggcgta ttcgaaccgc gcaagctgac
     7021 tgaagctgcg ctagcgctcg acagggccgc gggcgggtct atcagcaata tattgaatag
     7081 cggggatatg gacggtaaag ctgggactgc aagactgctg cataacgttc ccaacacgct
     7141 gtgcgaacgc gtgctgctag ttggccttgg gaaagaggaa gagctaagcg aaaaaagata
     7201 ccgcgacgcg attcgcgctg cgttcagggc attgaatgac accggtgcgg cggacgcaac
     7261 ttttttcctg acgggaatac ccgtcaggaa ccgggactct gcctggaaag tctcacatac
     7321 cgcgatcgta gcaatggaaa gcagctatcg tttcgaccgc ctcaaaagca aagtcgacga
     7381 taaaccgcct catctgcgca agattacgct aggaataaca acgggcgaag cgtcggaggt
     7441 acctgcggct gaatcggcct tgcaacaagg gattgccatt gccgaaggca tgcatcttgc
     7501 gaaggacctg ggtaatctcg cccccaatat ctgcacgccc acttatctcg ccgagcaggc
     7561 agcaggcctt gccaagacct acaagctgaa ggcgactatt ctcgaacaga aggatatgga
     7621 gaagctcggt atgggagcgc ttctcgccgt agcgcgcgga agccggcagc cggcaaagct
     7681 gatcgttctc gagtactggg ggcggtcaaa gaaggaaaaa ccagtcgtac tggtgggcaa
     7741 gggaatcact ttcgacaccg gcggcatttc cctcaagcct gcgccggaaa tggatgaaat
     7801 gaagtatgac atgtgcgggg cggccagcgt gcttggcgcc ataaacgcga ttgcaaaaat
     7861 gcggctgcct ttaaacgtgg tcggcatcat cccgactact gaaaacatgc cgggcgggaa
     7921 cgccaccaaa ccgggggatg ttgtcaccag catgtccggc caaacggtcg aaatcctcaa
     7981 cacggacgcg gaggggcggc tcatcctgtg cgacgcgctg acctatgccg agcgctttga
     8041 gccggacact gtcgtcgata tcgcgaccct gactggagct tgcgtcattg cgctgggtca
     8101 cgtcgcgtcc ggcctgttga cccccgatga tgaccttgcg gaggaattgc tccacgcctc
     8161 ggaacaggcg gctgatcccg catggcgctt gccactatgg gacgattacc aggaacagct
     8221 caaaagcaat tttgcggaca tggcgaatat tggcggcaga gcggccggaa ccattaccgc
     8281 cgcatgcttt ctctggcgtt ttaccaagaa ataccgctgg gcccatttgg acatagccgg
     8341 gactgcatgg aaatccggca aggaaaaagg ctctacggg
//
LOCUS       DASSHJ010000030         7036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1179554,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000030 DASSHJ010000000
VERSION     DASSHJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179554"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1849
                     /gene="pgi"
                     /locus_tag="VFI43_01470"
     CDS             101..1849
                     /gene="pgi"
                     /locus_tag="VFI43_01470"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET7060827.1"
                     /translation="MLSFDQSEVVYYQAMLNPDPIDLQNQEETSGERSPDSGVSARSL
                     TQRPAWKALEKHYRAIHLVHLREFFANDPKRGERLTAEAAGLYLDYSKNRVTDETLQL
                     LVRLAEECGLRERIDAMFSGEHINVTENRAALHIALRAPREDRIFVDGVNVVPEVHAV
                     LDRMAAFSEQVRDGLWRGYTGKQIRNVINIGIGGSDLGPVMAYEALRYYSARDISFRF
                     VSNVDGTDFREATRDIDPEETLFIICSKTFTTLETLTNAQAARAWVLDRMKDAGAVRK
                     HFVAVSTNAEAVLQFGIDPSNMFEFWDWIGGRYSMDSAIGLSTMIAIGPENFHEMLTG
                     FHEMDEHFRTAPFERNLPVLMALLSIWYNDFFGAQTTAVLPYAEYLKRFPAYLQQLTM
                     ESNGKHVTVEGVRVDYQTSPIVWGEPGTNGQHSFYQLIHQGTRLIPCDFIGFCQTLQP
                     LGDQHDLLMANFFAQTEALAFGKTEDEVRSEGVPDWLCPHRSFEGNRPTNTILIDRLT
                     PHSLGTLVALYEHSVFTQGMIWNIDSFDQWGVELGKALAKRIVGELENTGTANPSLPD
                     HDSSTNALIRRYRGCK"
     gene            2041..4143
                     /gene="tkt"
                     /locus_tag="VFI43_01475"
     CDS             2041..4143
                     /gene="tkt"
                     /locus_tag="VFI43_01475"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7060828.1"
                     /translation="MPEQKTLDQICIDTLRSLSIDAVEKANSGHPGTPMDAAPTVYCL
                     WQRFLQYDPRDPEWLNRDRFILSAGHASALLYSLLYLAQVKAMAPSYETKDRLAVTLD
                     DLKTFRQLGSRCTGHPEYGWTSGVETTTGPLGQGAADSVGMAIAGFWLAATYNRPDFK
                     VFDYNIYALCSDGDLMEGVSSEAASLAGHLKLSNLCWIYDDNAITIEGSTNLTFTEDV
                     GARFESYGWEVAHVADANDLDALTNAYREAIDTRDRPTLIIVHSHIGYGAPHKHDTRE
                     AHGEPLGAEEVRLAKEFYGRDPDKQFYVPEGVVEHFDAQLGERGATLHDNWKMLFSDY
                     RARYPDLAEQIDYMQERGLPSGWDSKLPTFPADSKGMATRESSGKVLNAIAANVPWLL
                     GGAADLAPSTKTELKGEFFGDFQAPGQPAKGDYRGRNFHFGLREHAMCAIANGLSLSK
                     VRPYAASFLIFSDYCRPAIRLSAMMEIPVIYIWTHDSISLGEDGPTHQPIAHLAYFRA
                     SIGLIMFRPADANEVVESWRVVMGLRDRPACLVLTRQAVPTLDRSKLAPASGVSRGAY
                     ILADAPDGKPDVLLLSTGSEVSLCVTAYDQLKSEGIKARVISMPSWALFEEQSQEYRE
                     EVLPPHVLARVAVEEASSFGWERYTGMEGCIIGIDRFGLSAPGKVVAQHFGFEPQHIV
                     AAAKEQIARHASNSHQAD"
     gene            4209..5918
                     /gene="pgm"
                     /locus_tag="VFI43_01480"
     CDS             4209..5918
                     /gene="pgm"
                     /locus_tag="VFI43_01480"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HET7060829.1"
                     /translation="MKISPLAGKPAPPEMLVNIPRLVTAYYTQIPDVAVAAQRVMFGT
                     SGHRGSSLETSFNEWHVLAITQAICHYRNEQRITGQLFLGIDTHALSEPAYATAMEVL
                     AANGVEVRVAGNGEYTPTPAISHAILTHNRGRTEGLADGIVITPSHNPPESGGFKYNP
                     PHGGPASVEVTSSIESLANEYLKKRLAGVKRIPFEAALRASTTRHHDFLKCYVEDLGA
                     VIDMDVIRGSDIHMGVDPLGGAGVNYWPAIAERFKLNLETVRTEVDPTFRFMTVDWDG
                     RIRMDPSSRYAMQGLISLKDRFDIAFACDTDHDRHGIVTRSVGLMPADHYLSVAIDYL
                     FRNRRQWAGAVGVGKTVVSSRMIDKVTSRLARRLFEVPVGFKWFVDGLHDGSLGFCGE
                     ESAGASFLRFDGSVWTTDKDGIISALLSAEITACMGHDPGELYGGLERELGHSVFERL
                     DAPASPEERKILSNLSPEQIDCADLAGEKIESVLTRAPGNNAPIGGIKVIAKDGWFAA
                     RPSGTEDIYKIYAESFIGMDHLRHVIEQAQALVSRALATAAERDTQPEKRPAAETAVA
                     RTV"
     gene            5979..6578
                     /locus_tag="VFI43_01485"
     CDS             5979..6578
                     /locus_tag="VFI43_01485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET7060830.1"
                     /translation="MDDNCVIHECKVALLLIDVINDLEFPGGDKLVDSAEAAAENTAA
                     LKRQAKAHRVPCLYVNDNFGQWQSDFKKTVARCAREGVRGSGMSRKLAPEANDYFVLK
                     PRHSGFYQTPLDLLLRHLRAARLILCGLTTDSCVSFTANDAYLRGYEILVPEDCSAAI
                     SQSRHSGALQQMRNTLKARTEESGQIDFAKLLEKTGDPP"
     gene            complement(6737..6889)
                     /locus_tag="VFI43_01490"
     CDS             complement(6737..6889)
                     /locus_tag="VFI43_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060831.1"
                     /translation="MSIRRLESGVIIINQAQVAPRRGHVAIAALVATTAWTGEGLSSS
                     PGDPGY"
BASE COUNT         1598 a         1941 c         1988 g         1509 t
ORIGIN      
        1 tgattaaagt gcgggataac aaaaagctct gaatctgcgc cctcgcgcag tccgccctgc
       61 ggattattca cacctccttc acttttcgct gacacctgct atgctaagtt ttgaccaaag
      121 cgaggttgtt tactatcaag cgatgctgaa tcccgatcca atagatcttc aaaatcagga
      181 agaaacgagt ggcgaacggt cgcctgattc cggcgtatcc gcccggtctt tgacccagcg
      241 gcccgcatgg aaagcgctcg aaaaacacta tcgggcgatt cacctggtgc acttgcgcga
      301 gttttttgcg aatgatccca aacgaggtga gcgtttgacg gcggaagctg ccggtctcta
      361 cctcgattat tcaaagaacc gcgtcacgga tgagacactc cagttgctcg tccggcttgc
      421 cgaagaatgc ggtttgcgcg agcgcatcga tgccatgttc tcaggcgagc atatcaacgt
      481 caccgaaaat cgtgccgcac ttcatattgc gttgcgcgcg ccgcgggaag accgcatctt
      541 tgtggatggc gtgaatgtag taccggaggt gcacgccgtc cttgaccgca tggctgcgtt
      601 ttccgagcag gtgcgcgacg gactctggcg ggggtatacc ggcaagcaaa tccgcaatgt
      661 catcaatata ggaatcgggg gctccgacct cggcccggtc atggcctacg aagcgctgcg
      721 ttattacagt gcgcgcgaca tcagtttccg cttcgtatcc aatgtcgatg gtacggactt
      781 cagggaagcg acaagggata tcgacccgga agaaacgctt ttcatcatct gctcaaagac
      841 gttcaccacg cttgaaaccc tcacgaatgc ccaagcggcc cgggcatggg ttctcgacag
      901 gatgaaggac gcgggggcag ttcgtaaaca tttcgtcgct gtttcaacaa acgccgaggc
      961 agtacttcag ttcggaattg acccttccaa catgtttgaa ttctgggact ggatcggggg
     1021 acgttactcg atggactccg cgatcgggct ttcaaccatg atcgcgattg gcccagaaaa
     1081 ctttcacgaa atgttgaccg ggtttcatga aatggacgag cactttcgca cggctccttt
     1141 cgaacggaat ctgcccgtac tcatggcgtt gctctccatc tggtacaacg atttcttcgg
     1201 ggcgcaaacg accgccgtgc tgccttacgc cgaatatctc aagcgcttcc ctgcttattt
     1261 acagcaattg acgatggaga gcaacggcaa gcatgtcacg gtcgagggtg ttcgcgttga
     1321 ctatcagact tctcccatcg tttgggggga acccggcacc aatgggcagc attcatttta
     1381 ccagctcatc catcaaggaa cccgcctgat cccctgcgac ttcatagggt tctgccagac
     1441 attgcagccg ctgggggatc agcacgatct cctcatggca aacttttttg cccaaacgga
     1501 ggccctcgca tttggaaaaa ccgaggatga agtacgatca gagggtgttc ctgattggct
     1561 ctgcccccac cgcagctttg aaggcaatcg cccgaccaat acgatactga tcgatcggct
     1621 gacgccgcat tcgctcggga cgctggttgc tttatacgag catagcgtct ttacgcaggg
     1681 aatgatctgg aatatcgact cgttcgatca gtggggggtg gaactgggga aggcactggc
     1741 caagcgaatc gtgggagaac tggagaacac aggaactgct aatccttctc tcccggatca
     1801 tgacagctcc accaatgcct tgatcaggcg ctaccgcgga tgcaaataag caatcacgcg
     1861 actggaaccg ggtatagaag attcgggagg gtggggagct tataaagaac tgatgggtta
     1921 gggctacaga tagggctgct tggtttgggg cgacttcatg aagcgctttt taacagtaac
     1981 acgaggcgca ataaaaggtc ttcaatctaa tacagaaata tctttctgtt ggaatatgtc
     2041 atgcctgagc aaaaaacact agaccaaatc tgcattgata cactacgcag tctttccatc
     2101 gatgcggtgg aaaaagccaa ctcaggtcat cccggcacgc ccatggatgc tgcccccact
     2161 gtctattgcc tgtggcagcg cttcctgcaa tatgatcctc gcgatccaga gtggctgaat
     2221 cgcgaccgtt ttatcctctc cgccggtcac gcctccgctt tgctttacag tcttctctat
     2281 ctcgcccagg ttaaagccat ggccccgagt tacgaaacga aagacaggct ggcggtgacg
     2341 ctcgatgatc tcaaaacttt ccggcagctt ggcagccgct gcacaggtca ccctgagtac
     2401 ggctggacct ccggggtgga aacgacgacg ggcccgctcg gacaaggcgc agccgatagc
     2461 gtcgggatgg caatcgccgg cttttggctg gccgccacct ataatcgtcc ggatttcaaa
     2521 gtattcgatt acaacattta cgcgctctgt agcgatggtg acttgatgga gggcgtcagt
     2581 agcgaggccg catcactcgc cggtcatctc aagttatcca atctctgctg gatttacgat
     2641 gataacgcga tcaccatcga gggatcgacg aacctcacat ttacggagga tgtgggcgcc
     2701 cgatttgaaa gctatggctg ggaggttgcg catgtagccg atgcaaatga cctcgatgcg
     2761 ctgacaaacg cctatcgcga ggctatcgac acgcgtgacc ggcctacgct cattatcgtg
     2821 cacagccaca tcggctatgg cgcgccgcac aagcatgaca cgcgtgaggc gcatggagag
     2881 ccgctgggtg cagaagaagt ccgtctggca aaggaatttt acggccgcga tccggacaag
     2941 cagttctatg tgcccgaggg cgtggttgag cacttcgacg cacagcttgg cgagcgagga
     3001 gcgaccttgc atgacaactg gaaaatgctg ttttcggact atcgtgcgag atatcccgat
     3061 ctggcagagc agatcgatta catgcaggag cgaggactgc cctcgggctg ggatagcaaa
     3121 ttgccgacgt ttccggcgga cagcaaaggg atggcgaccc gcgaatcatc gggaaaggtt
     3181 ttgaacgcga tcgctgcaaa tgtcccatgg ttgctcggag gtgcggcaga tcttgctccc
     3241 tccacgaaaa cagagctgaa aggcgaattt ttcggcgatt ttcaagcgcc cgggcaaccg
     3301 gcgaaaggtg actatcgtgg ccggaatttt cacttcggct tgcgggaaca tgcgatgtgc
     3361 gccattgcaa atggcttgag cctgtcgaaa gttcgccctt acgctgcaag ttttctcatt
     3421 ttttccgact attgccgccc tgcgatccga ttgagcgcca tgatggaaat acccgtcatt
     3481 tacatctgga cgcatgactc gatcagcttg ggagaagatg gcccgaccca tcaacccatc
     3541 gctcacctgg cctactttcg ggccagcatt ggtttgatca tgttcaggcc ggcagatgca
     3601 aatgaggtgg tcgagtcctg gcgagttgtc atggggctgc gggatcgtcc cgcttgtctg
     3661 gtgctgaccc ggcaagccgt gcccaccctg gatcggagca agctcgcccc cgcgagcggc
     3721 gtttcgcggg gagcctatat tctcgccgac gcccccgatg gaaaaccgga cgtgcttctg
     3781 ctttcgacgg gaagcgaggt ttcactctgc gtgaccgctt acgaccaact caagtctgag
     3841 gggatcaagg cacgcgtgat cagcatgccc tcctgggcat tgtttgagga acagagccaa
     3901 gaatatcgtg aagaggtgtt gccaccccac gtccttgcgc gtgtcgccgt tgaggaagcg
     3961 tccagtttcg ggtgggaacg ctataccggg atggaaggat gcattatcgg aatcgatcgc
     4021 ttcgggctgt ccgctcccgg aaaagtcgtt gcccagcatt tcggattcga gcctcagcat
     4081 atcgttgccg cagccaagga acagattgcg cgtcatgctt caaactccca tcaggcagac
     4141 tgaaaaaaac acgctcggaa tcccttgcct cgcccgtgcc cggcaagccg cctaacccag
     4201 gatgattcat gaagatcagt cccctcgcag gaaagcccgc ccccccggaa atgctggtca
     4261 acattccccg gcttgttacg gcgtattaca cacaaatacc tgacgtagcg gtggcggctc
     4321 agcgtgtcat gttcggaacc tcgggacatc gaggctcttc gctggaaacg agcttcaatg
     4381 aatggcatgt tctggcaatt acccaagcca tttgccacta tcgcaacgag caacgcatta
     4441 ctggtcagct ttttcttggc atcgacacgc atgcgctttc cgagccagcc tatgcaaccg
     4501 ccatggaagt gctggctgcc aacggtgtcg aggtaagggt ggcaggcaac ggcgaatata
     4561 cgcccactcc agccatatcc cacgcaatac tgacgcacaa tcgtggacgt acggagggcc
     4621 ttgcggacgg gatcgtcatc acgccctcac acaacccccc cgagagcggg ggcttcaaat
     4681 acaacccgcc acatgggggc cccgcgagcg tggaggtaac ctcgtctatc gagtcgctcg
     4741 ccaatgaata cttgaagaaa aggctcgcgg gagtaaaaag aattccattc gaggcggctc
     4801 tgcgggcctc gaccacccgc catcatgact tccttaaatg ttatgtcgaa gatctcggtg
     4861 cggtgatcga catggacgtc atccggggat cggacattca tatgggcgtt gatccgctcg
     4921 gcggtgcagg agtgaattat tggcccgcca ttgcagagcg cttcaaactg aatctcgaga
     4981 cagtaagaac ggaagtggat ccaaccttcc gcttcatgac tgtggactgg gacggaagaa
     5041 tccggatgga cccgtcgtcg agatatgcaa tgcaggggct gatcagcctg aaagaccggt
     5101 tcgacattgc tttcgcctgc gatacagatc acgatcgcca tggaatcgtg acccgaagcg
     5161 tgggtctgat gccggcggat cactatcttt ccgttgccat cgattatctg ttccgcaacc
     5221 gcaggcaatg ggcaggcgct gtgggagtgg gcaaaacggt ggtcagcagc cgaatgatcg
     5281 acaaagtgac ttcccggctt gcccggcgtc ttttcgaagt gccggtcggc ttcaaatggt
     5341 tcgtggatgg gctacatgac gggtcgctcg ggttctgcgg cgaagaaagt gctggagcct
     5401 cattcctgcg cttcgacggc tccgtatgga ccacagacaa ggatgggatc atctcggcgt
     5461 tgctctccgc ggaaattact gcctgtatgg ggcatgaccc gggcgagctg tacggcgggc
     5521 ttgaacgcga gctgggtcac tcggtattcg agcgcctgga tgcgcccgcc tctcccgaag
     5581 aaaggaaaat cctgtcgaac ctgtcccccg agcagatcga ctgtgccgac cttgccgggg
     5641 agaaaatcga gagtgttctc acgcgtgctc caggcaataa tgcccctatc ggtggaataa
     5701 aggtgattgc gaaagacggc tggttcgctg cccggccctc cggaacggaa gatatctaca
     5761 agatctatgc ggagagcttc atcgggatgg atcacctgag acacgtcatt gagcaggcgc
     5821 aagcgctggt ttcccgtgca ctcgccactg cagccgagag ggatactcaa cctgaaaagc
     5881 ggccggctgc cgaaacagct gtcgcgcgca ccgtctgatc caacccgttc tccaggataa
     5941 gcagggggtg cagttgatga tggatttgag aatcgggaat ggatgacaac tgtgttattc
     6001 acgaatgcaa ggtcgcactt ctcctgatcg atgtcatcaa cgatctggag tttccaggag
     6061 gtgacaagct ggtggatagc gcggaagctg cggcagaaaa taccgcggcc ctcaaacgcc
     6121 aggcaaaggc acatagggtg ccctgtttgt atgtaaatga taacttcggc cagtggcagt
     6181 ccgattttaa aaagaccgta gcgcgttgcg cccgcgaggg cgtgcgcgga tccgggatga
     6241 gccggaaact ggctcccgaa gcaaacgatt acttcgttct caagccccgc cattccggct
     6301 tctaccaaac gcccctggac cttttgttgc ggcatttgcg ggccgcgaga ctgattctct
     6361 gcggattgac aactgattcc tgcgtttcat ttactgccaa cgacgcgtat ttacgtggat
     6421 acgaaattct tgtccccgag gattgcagcg cggcgataag ccaaagcagg cactccggcg
     6481 cgctccagca gatgcgaaat actctcaagg cgcgcacaga agaatccgga cagatcgact
     6541 ttgcaaaatt gctggagaaa accggagatc caccctagtg acagcatgtg cctccttctc
     6601 ggctgagaca atcgccagat tgaactgaat cggaaacgct tcctgctcgc tctccgagcc
     6661 gcgctgcttg aaaagccagc aggaacaaac gaaggggcag aggctgtttc agaagcgaca
     6721 gacggattca gatcgatcaa tatcccggat cgcccggcga agacgagagg ccttccccgg
     6781 tccaagcagt ggtcgctaca agggcagcga tggcgacatg gcctcgacgg ggcgccactt
     6841 gcgcctggtt gatgattatc actcccgatt ccagacgccg gatcgacatg gcttccagtt
     6901 cgccgaaaaa caagcttgtc atttatgtag ccattgcagg caatcttgcc attgccattg
     6961 tgaaattcat tgcagccggc ctcagcggca gctccgccat gttatcagag ggtattcact
     7021 cgatggtcga tacagg
//
LOCUS       DASSHJ010000031        26405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_89216, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000031 DASSHJ010000000
VERSION     DASSHJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 26405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26405
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89216"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFI43_01495"
     CDS             <1..345
                     /locus_tag="VFI43_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060832.1"
                     /translation="YRKLVDMVVDDGEGEAAVQQVVRFVHPRLRGTLAALELRRAAAP
                     ITYESLKEIVNHWAATAMTLTDRDLRLMERLARAQIRKIGGFGDGAIEEIKKLELSIA
                     WERHHVAPWTIS"
     gene            427..2013
                     /locus_tag="VFI43_01500"
     CDS             427..2013
                     /locus_tag="VFI43_01500"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7060833.1"
                     /translation="MTHGAATTLTMATIIEDHAHKRPHQTAIIYNDHRFSYGQLNAMA
                     NMIANGLVELGVKPGDHVAISCPNLPYFPMVYFGILKVGAVVVALNVLLKPREIAFHL
                     QDSDAKAFICFEGTAELPMGQMGKAGFDAVPECRTMIMITANPASPSPTEGVKTLGQL
                     MHNQLPMFATYKPKPTDTAVIFYTSGTTGRPKGAELSQINLLMNAVVVRDLAASAMEL
                     GADAQNVVLITLPLFHATAQQAQMNANLLAGATLTLMPRFEPGSVLEVMKRDKVNLWT
                     GVPTMFHALLDYATANSIDIVPVAASLRLISSGGAPMPVELMNRFQDTFGVRVLEGYG
                     LSEASPVVSFNHADTPSKVGTVGQALWGVDIRCVDDEDEPVPIGEKGEVVIRGHNIMK
                     GYYKQPEATAEAMRNGWFHTGDIGILDADGYLSIVDRKKDMIIRGGYNVYPRELEEVL
                     STHPAVSAVAVVGIYHEKLGEEVKAFVVKKTDSEITEDELIAWCKEEIAAYKYPRHIE
                     FRDQLPMNATGKILKRELRQ"
     gene            2303..3115
                     /gene="dapD"
                     /locus_tag="VFI43_01505"
     CDS             2303..3115
                     /gene="dapD"
                     /locus_tag="VFI43_01505"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET7060834.1"
                     /translation="MCELESTIERAWAAGVSTSHSQAVRDAVETTLSGLDCGRIRVAE
                     RQGVGQWAVNQWVKKAVLLSFRLSDNKLLRAGDLSFFDKVASKFANLNEDDMHASGVR
                     VVPPAVVRRGSFLAHNVVVMPSFINIGAYVDEGTMVDGWANVGSCAQIGKNVHIAAGV
                     CIGGVLEPMQANPTIIEDDCFIGANSSVVEGVIVEENSVIAMGVHLGQSTKIIDPETK
                     QISYGRIPAGSVVIGGTLPSTDGSYSTACAVIIKRVDAQSRAKTSINELLRT"
     gene            complement(3197..3727)
                     /locus_tag="VFI43_01510"
     CDS             complement(3197..3727)
                     /locus_tag="VFI43_01510"
                     /inference="COORDINATES: protein motif:HMM:NF022152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HET7060835.1"
                     /translation="MTLTGDPFSSVQLVGYGAMIVSLGAYAMREDKMMKTGVGVGLVL
                     WALHYAMLLAWTASAICLLIASRQILAFLAPAMSAAGRRATAVSYCLVFTGVLWETWA
                     GPVSLLPWISAINATYAYFYLAGTRLRARVMLSTGLWLLNALALGSIGGVAMNAASLG
                     ISLWTIARLKRTAPVL"
     gene            4041..4604
                     /locus_tag="VFI43_01515"
     CDS             4041..4604
                     /locus_tag="VFI43_01515"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HET7060836.1"
                     /translation="MKGKKVWERGSILVFIFSGSVAAGVPEGPFSHNYDIGTLTSTPY
                     ENVFSGETNLLSHSYTFDLDAPATVDARAVNARMETGFIGGLFPVTHTLFDISLFDSG
                     NHKLYEGTTTQAWTFGSTLEAAVSGELPAGKDYFVLITGSQTNDTALAYAIKISVIPT
                     TVPEPETYAMFLAGLGMLVWRIRRQRS"
     gene            4881..6050
                     /gene="carA"
                     /locus_tag="VFI43_01520"
     CDS             4881..6050
                     /gene="carA"
                     /locus_tag="VFI43_01520"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7060837.1"
                     /translation="MSQHPHAVLALADGTVFRGSSVGAEGVTVGEVVFNTSMTGYQEI
                     LTDPSYCQQIITFTYPHIGNTGVNGEDVESGNINRVYAAGMVIRDLPLVSSSWRSTGD
                     LPGYLKRQGVIAIADVDTRKLTRILREKGAQAGCIVAGEAFSQADAVHRARQFPGLSG
                     MDLAKVVSCAEPYEWTEGEWSLREGRKPRKDTSFHVAALDFGIKQNILRKLAQRGCRV
                     MVLPAQTSADEVLQLKPDGVFLSNGPGDPEPCDYAITATREILQAEVPIFGVCMGHQL
                     LGLAIGARTVKMKFGHHGANHPVQDLDTGRVLITSQNHGFAVDTGSLPDYARVTHVSL
                     FDGSLQGFELIGKNVFCFQGHPEASPGPHDLDYLFDRFVASMENRKKEGIQTRVK"
     gene            6183..9440
                     /gene="carB"
                     /locus_tag="VFI43_01525"
     CDS             6183..9440
                     /gene="carB"
                     /locus_tag="VFI43_01525"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7060838.1"
                     /translation="MPRRNDIKSILILGAGPIVIGQACEFDYSGVQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIIWPMVAKIIAAEHPDALLPTMGGQTALNCALDL
                     AKNGVLDQYGVELIGASREAIDKAEDREKFKQAMTRIGLGSARSAIAHSIEEALQVQA
                     MVGYPAIIRPSFTLGGSGGGIAYNREEFLDICERGLEASPTNELLIEESVIGWKEFEM
                     EVIRDRKDNCIIVCAIENLDPMGVHTGDSITVAPAQTLTDKEYQILRDASIAVLREIG
                     VETGGSNVQFAINPKDGRMLVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNDITGGMMPASFEPVIDYVVTKIPRFAFEKFSQANDRLTTQMKSVGEVMAIGRTFQ
                     ESLQKALRGLEIGADGLDEKTTDIDVITAELGDPGPERIWYVADAFRCGMPLEEIHEH
                     THIDPWFLVQIEDLVKQEQSVLGKDLGSLDRLELFKLKRNGFSDQRLAKLMRTTQGAV
                     RSRRHQLELRPVYKRVDTCAAEFATQTAYMYSTYEEECEALPSDRKKIIVLGGGPNRI
                     GQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     FVEKPLGVIVQYGGQTPLKLARDLEANGVPIIGTSPDMIDCAEDRERFQKMLHQLGLK
                     QPPNRTARNPEAALAAAREIGYPLVVRPSYVLGGRAMQIIHEQTHLERYMQEAVKVSH
                     DSPVLLDRFVNDAVEVDVDAVCDGETVLIGGIMEHIEQAGVHSGDSACSLPPFSLSKP
                     LQDELRGQTAAMARALGVVGLMNVQFAIQGETVFVLEVNPRASRTVPFVSKATGLPLA
                     KISARCMAGISLVRQGVVKEIIPSYYSVKEAVFPFIKFPGVDPILGPEMKSTGEVMGV
                     GQTFAEAFVKSQLGAGVKLPRKGKVFISVRQVDKPRAVEIARNLAQLGFTLYATRGTA
                     TVLSSAGLTVTPVNKVAEGRPHIVDMIKNGEMDLIINTVEDKRSAVHDSYSIRNAVLQ
                     ARVTYYTTLAGARAACIGMANMGELRAYSLQELQGSAAEAPCEASDEGHLLVKQF"
     gene            9461..9937
                     /gene="greA"
                     /locus_tag="VFI43_01530"
     CDS             9461..9937
                     /gene="greA"
                     /locus_tag="VFI43_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7060839.1"
                     /translation="MGKIPLTVIGAEKLRAELHELKTVQRPAVITAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRIAELETKLSNAQIIDPALLDAGGACVFGATIDLEDMTNSTT
                     VTYQIVGDDEADIKAGKISVSSPISRALIGKYPGDVAEVIAPGGVREYEILDVKYV"
     gene            complement(9999..10409)
                     /gene="yhbY"
                     /locus_tag="VFI43_01535"
     CDS             complement(9999..10409)
                     /gene="yhbY"
                     /locus_tag="VFI43_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET7060840.1"
                     /translation="MLELSSSHRATLKALSHSLKPVVWIGGGGLSEAVIRELDQALKS
                     HELIKIKVSNDERETREALLEQICGRLRAAPIHHIGRMFVIYRPVPNEIPVENIRKTT
                     SGATSKGKSRELIASANKDPSLRPKRKMAPRRGR"
     gene            10452..11072
                     /locus_tag="VFI43_01540"
     CDS             10452..11072
                     /locus_tag="VFI43_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET7060841.1"
                     /translation="MKRTKTSKAWMKEHVNDFFVKQAKKEGYRSRAAYKLVEIAERDR
                     LIAPGMTIIDLGAAPGGWSQVAAEKLAGTGMVIAVDLLEMQAIPGVLFMQGDFREPQV
                     LAEVKKKLEGRPPGLVISDMSPNITGISIVDQARGMELAELALDFSVDQLNSGGNFLV
                     KVFQGSGFEAFLNAMRTTFDRVVTRKPGASRDRSSEIYLLGLGKRG"
     gene            11192..13087
                     /gene="ftsH"
                     /locus_tag="VFI43_01545"
     CDS             11192..13087
                     /gene="ftsH"
                     /locus_tag="VFI43_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7060842.1"
                     /translation="MNNLIKNMAIWLVIALVLMTVFNQFSTRQSAQAPMEYSQFIEEV
                     KQGRIAKVTIEGRTIKGTKSDGRRFTTYTPSDPWMVSDLLKAGVIIDAKPEEEPSLLM
                     NIFVSWFPMLLLIGVWIFFMRQMQGGGRGGGAFSFGKSKARMLDESTNQVTFADVAGC
                     EEAKEEVSELVEFLRDPSKFQKLGGRIPRGVLMVGNPGTGKTLLARAIAGEAKVPFFS
                     ISGSDFVEMFVGVGAARVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGLGGGNDEREQ
                     TLNQLLVEMDGFEGTAGVIVIAATNRPDVLDPALLRPGRFDRQVTVPLPDIRGREQIL
                     HVHMRKVPIAPDVHAEIIARGTPGMSGADLANLVNEAALFAARSNKRLVDMDDFERAK
                     DKIFMGAERRSVVMPERERRNTAYHESGHAVVAQLLPKTDPVHKVSIIPRGRALGVTM
                     QLPTEDRFSMDREEILQSIAVLFGGRIAEEIFMGQMTTGASNDFERATEMARRMVTQW
                     GMSDQLGPMVYGENEGEVFLGRSVTTHKNVSEATMQKVDIEIRRIIDAQYGLARKLIE
                     ENRDKIEVMAQALLEWETIDSDQIGDIMAGRTPRPPRPTKVTSTPPKDNTPPGAPAAT
                     ATPAQEV"
     gene            13235..14032
                     /gene="folP"
                     /locus_tag="VFI43_01550"
     CDS             13235..14032
                     /gene="folP"
                     /locus_tag="VFI43_01550"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7060843.1"
                     /translation="MGIINVTPDSFSDGGLYASLGQALRHAELLFEEGADLLDVGGES
                     TRPGSIPVSVDEELRRIMPVVDGLVRMNVPVSVDTTKPEVMRAAIRAGAVMINDVNAL
                     RAEGALEAAASGDVAVCLMHMQGEPRSMQVQPRYNDVVKEVKDFLRQRLDAARASGIP
                     DKRLVVDPGFGFGKTLEHNLELLRNLDQFADLGVTLMAGLSRKSMLGKITGNDVAARV
                     HASIAAALLAVAKGARIVRVHDVKATREALAIYNAVNNPVPGEEGWS"
     gene            14128..15489
                     /gene="glmM"
                     /locus_tag="VFI43_01555"
     CDS             14128..15489
                     /gene="glmM"
                     /locus_tag="VFI43_01555"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7060844.1"
                     /translation="MTRKFFGTDGIRGRVGEVPITPEFVMHLGYSAGKVLASANWHLA
                     QGERPAVLIGKDTRISGYMLESALQAGLSAAGVDVLLSGPTPTPAVAYLTRALRLQAG
                     IVISASHNPFDDNGIKFFSASGTKLPDSVEHEIEAGLNQPIKSMPSAQLGKARRVNDA
                     RGRYIEFCKSTFPNHLDLRGFRIVVDCAHGATYQIAGHVLHELGAEVVAIGAQPDGLN
                     INHECGATHSATIGNAVKQYKADIGIALDGDGDRVIMADNNGVLYDGDQLIYIIAVHR
                     KRNGLLRGGVAGTLMTNLAVENGLKKLNVPFARANVGDRYVLELMQKNAWQLGGEGSG
                     HIICTDKHTTGDGIISALQVLYAVRDSSSTLAELARDVTLYPQQLINVKVPKGFDSGK
                     SAVIKNVQAEAEQDLNGSGRVLLRASGTEPLIRVMVEGQSSQKVSHWAEKIADAVRTA
                     AAS"
     gene            15626..16627
                     /locus_tag="VFI43_01560"
     CDS             15626..16627
                     /locus_tag="VFI43_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7060845.1"
                     /translation="MLKLPRWMLGISVAAAALIAGISMAASGRMVKIDGSSTVFPITE
                     AVAEDFQNAKRGAVRVTVGISGTGGGFKKFCRGEIDIVDASRPILEKEMADCRAAGVQ
                     YVEMPVAFDALTVVVNPKNTWSNVITVAELKKLWEPAAQRTIMRWNQVNPAWPDEPIK
                     LYGAGADSGTFDYFTEAIVGKAKSSRGDFTASEDDNVLVQGVASDKYGLGFFGFAYYI
                     ENQKKLKAIAVDAGKGPVMPSARTVEDGTYQPLSRPIFIYVNVKSAERPEVRELVEFY
                     MKNGPELVKEVKFFPLPSKVYTTNLEHLDKKKVGTVFGGKAEVGLRIEEVLKREAKL"
     gene            16802..18544
                     /gene="argS"
                     /locus_tag="VFI43_01565"
     CDS             16802..18544
                     /gene="argS"
                     /locus_tag="VFI43_01565"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7060846.1"
                     /translation="MSPPGYPNFKFHFTELLRQLLSGMLPKGLEPDIEFARPKQANHG
                     DYSCNIAMQLAKPLRRSPRDIATLLAEGLAASPYLEKVEVAGGGFINLFLTLSAKQQF
                     PNYVLSEGEGYGRSNIGASRKIQVEFVSANPTGPLHVGHGRGAAFGASLANVLEAAGY
                     SVTREYYVNDAGRQMDILALSTWLRYLQLNGLAISFPPNAYQGEYVRDMARLIYQAHA
                     ARYVHEPELLFEGIGHEAEGEARLDHLIANAKKLLGEDYAYIHNFVLAEQLGDCRNDL
                     MEFGAVFDVWFSEQSLFDSGSVSRAIDFLDQSNHIYQQDGAKWFKSTDFGDEKDRVVQ
                     RENGQFTYFASDIAYHLNKFERGFERVIDIWGADHHGYIPRVKGAMKALSLDPEKLEI
                     ALVQFAILYRDGKKASMSTRSGEFVTLRELRQEVGNDAARFFYVLRKSDQHLDFDLDL
                     AKSQSTDNPVYYVQYAHARICSVLEQWAGDASVLARADMSPLTGAAELSLLQKLIDYP
                     ELLESAARELAPHLVAFYLRELAGEFHSYYNSTRFLVPEVPVRLARLGLAAALRQVLG
                     NGLKLLGVSAPAKM"
     gene            18568..19167
                     /locus_tag="VFI43_01570"
     CDS             18568..19167
                     /locus_tag="VFI43_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7060847.1"
                     /translation="MSRDYKTSPNSLNPFKGRNSLVIGIFIGYALGLLTAIATWMYIN
                     KGPSPFISADKPADSATAKSGAGIPQKGAQGLASPDKSTDGKPRFEFYNMLPNEGETI
                     TEQQLKQGAQQPASKDKYFLQAGSFQNASDADNLKAKLAMLGVEATVQAVNVPEKKGT
                     WHRVRVGPFSSIDDLNQVRASLQQNGVQSSVIKVQDGKQ"
     gene            19233..19883
                     /locus_tag="VFI43_01575"
     CDS             19233..19883
                     /locus_tag="VFI43_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HET7060848.1"
                     /translation="MKRMRLFALILFFAGTTFATAFTAHAELVEGRDYTVLRNPQPTH
                     SDNNVEVIEFFWYGCPHCYQLHPYISEWLKRKPKDVSFRYVPAIFRPSWVPAARIFYA
                     LEVLGARDKLHDKVYDAIHVEKIDLNKEDVLFDWVAKQGIDRQKFIDAYNSFSVQNQD
                     IPRSTNMSKSYGLTGVPAVVVDGKYMTSGRLGGTPEDTIRIMEELIEKARAERVKK"
     gene            20011..20490
                     /gene="msrA"
                     /locus_tag="VFI43_01580"
     CDS             20011..20490
                     /gene="msrA"
                     /locus_tag="VFI43_01580"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7060849.1"
                     /translation="MATEKAIFGAGCFWGVEAAFRSVKGVREAISGYAGGHTPNPTYP
                     EVCTGETGHAEAVQVEYDPSQVSYDRLLDVFWNCHNPTTRNRQGPDIGTQYRSAIFFY
                     TPEQEEAAKASKEMLQRGGRWQAPVVTEILPALEFYPAEEYHQRYFEKHGIAGHCHT"
     gene            complement(20628..21884)
                     /locus_tag="VFI43_01585"
     CDS             complement(20628..21884)
                     /locus_tag="VFI43_01585"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HET7060850.1"
                     /translation="MKTYLVGGSVRDEILGLPVQDHDYVVVGSTPEEMLSLGYRPVGK
                     DFPVFLHPESQEQYALARTERKISRGYKGFEVYASPEVTLQEDLARRDLTINAIAKDQ
                     SGVIIDPFAGIADLEAGVLRHISPAFNEDPVRVLRTARFAARFGFRIAPETLALMNEM
                     VHNGEIDSLVPERVWQEFARGLMEEKPSRMFYALRECNALIRIMPEVDALFGVPQPIK
                     YHPEVDTGVHVMMVIDHAAAKNYSLPVRFAALTHDLGKGTTPLEEWPHHYGHEARSVR
                     LVRGLCERIRVPNEARDLALLVARYHGDVHRALELRASTIARILQGVDAYRKPERFEE
                     FLQACSCDFHGRPGYAEKSYPQAQRLSEALLAARSVDAGAIAAATAGTVSEASRLPDV
                     INAKVSEARIAAIRNRLSMLDQQAVE"
     gene            complement(21914..22852)
                     /locus_tag="VFI43_01590"
     CDS             complement(21914..22852)
                     /locus_tag="VFI43_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET7060851.1"
                     /translation="MAVNSVCVFGGTGFVGGHLANFLTAEEILLRIPARSRERSKELL
                     VLPTVEVVQADVYDDSTLDRLLAGMDAVINLVGVIHGDFQAAHVELPRKIVAACQRNG
                     IRRLLHMSALNAGEGRPSAYLRTKGEGERIVKSSGLETTVFRPSVIFGPGDSSLTLFA
                     RLGRFPILALASPAAKFQPVFVEDVVRAFAASLDAPHTIGKSYDLCGPKCYSLRQMVE
                     YAAQATGGEPTIIGLNSSFSYLEALLLEFLPGKLMTRDNYFSMKIDNICDCEGYSVLE
                     DVWAIHPVAMEEVAPFYLTGELPRERYDEFRQRAGR"
     gene            complement(22869..24797)
                     /locus_tag="VFI43_01595"
     CDS             complement(22869..24797)
                     /locus_tag="VFI43_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7060852.1"
                     /translation="MKKFVAGLSLVLYAGALHAATDDDFMSMRQAFQKGDRARVSFYA
                     QRLQGHMLEPYAAYYQLRPQLEDSVLSPDSVKNFLLRYQDTPVADRLLLEWVKILGRN
                     EQWDLFGETYAGVTSLDTELACYSYRHRVAAGDAIALAEARPLWFNPRDLPESCAPLF
                     DTLVNAGVLTVEDIWARLRLALEAGNVGVAKRINEYLPDTEALDEGRLSNAQEGPLRY
                     LENHRNEMARRADREIALFAMLRMSRISPDQAYVYWPRVREQFNEAERSYFLVQLADQ
                     AARRLNPRALEWFAEAGRSTQPAPLSDAQLGWKVRSALRAGNWALVLDTIQAMSEEEQ
                     GTAVWRYWKARALKADNKVIEANAIFAPLSVEHHFYGQLAEEELGLTLQAPIEPLKIT
                     REDVSRIERLPGIRRALALYRLNLRTEASREWGWAIRDFDDRRLLAAAEVARQNGIYD
                     RAIHTADKTTQNHDFSLRFLAPHRDTLRAILKQQELDEAWVYGLIRQESRFVTDARSS
                     AGAMGLMQLMPATAKWAAKRMGMRKHPKTLSEVNTNLKLGTYYLKHVLDLFDNHPLLA
                     SAAYNAGPSRALQWRDDKPLEGAIYAESIPFSETRDYVKKVMSNSTYYARTFGHQLPP
                     LKQRLGIVPPRVSLEKFQ"
     gene            24916..26280
                     /locus_tag="VFI43_01600"
     CDS             24916..26280
                     /locus_tag="VFI43_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET7060853.1"
                     /translation="MPYISLNPATNKTIKTFTSWDSRRLAAALKEADNAGRLWAERSF
                     GERAECMHRAATLFHERVETYAQLMAVEMGKPLREGRAEVEKCALTCDYYAVHAEHFM
                     QTELVQTDASKSFVCYAPLGVLLAVMPWNFPFFQVIRFSAPALMAGNACVLKHASNVP
                     QCALALEQLFQDAGFPPHLFTTLMIEASDVADAIASRHVHAVTLTGSEAAGREVASHS
                     GQHLKKCVLELGGSDAFVVLKDADLDLAATFAVKSRFQNCGQSCIAAKRLIVVPEIAD
                     EFVSVFIEKAGDLKMGDPLNEATQIGPMARLNLRDGLHQQVMDSIAQGAQPVLGCQLP
                     PGEGSFYPVSVLDHVTARTRAYHEELFGPVAAVIRVGNEEEAISIANDSRYGLGSSIW
                     SRDIERAQKLSEEIEAGCCFVNGMVRSDPRLPFGGVKASGFGRELSYHGIREFVNIKT
                     IWVR"
BASE COUNT         6175 a         6868 c         7525 g         5837 t
ORIGIN      
        1 tatcgcaagc ttgtggatat ggtcgtggat gacggggagg gagaggccgc agtacaacaa
       61 gtcgttcgct tcgtccaccc gcggctgcgc ggcactttag ctgccttgga attacgtcgc
      121 gccgccgcac ccattaccta cgaatcattg aaggaaatag tgaatcattg ggcggcgacc
      181 gcaatgacgt tgaccgaccg cgaccttcgt cttatggagc gccttgctcg ggcacagata
      241 cgcaaaatag gaggtttcgg ggacggagcc atagaggaga tcaagaagtt ggaactctcc
      301 atcgcatggg aacggcacca cgtcgctcca tggacaattt catgaatgat gcctatcagc
      361 tctgctacac ctgcatattc aggagcgata tgggatacct tttttaactt aatttgagga
      421 ttgctgatga ctcacggggc cgctacaacg ctgactatgg ctactattat tgaagatcat
      481 gcgcacaaac gtcctcacca gacggcaatc atctataacg atcaccgttt ttcttacgga
      541 cagttgaatg cgatggccaa tatgatcgct aacggtcttg tagagcttgg ggtaaagccc
      601 ggagatcacg ttgccatctc atgtccaaat ttgccgtatt ttccaatggt ctattttggg
      661 attctcaagg tcggagcggt ggttgttgcc ttaaatgttt tgctcaagcc acgtgaaatt
      721 gccttccatc tgcaagacag tgatgcgaag gcttttattt gttttgaggg caccgccgaa
      781 ttgccgatgg ggcagatggg gaaggcaggg tttgatgccg tgccggaatg ccgaaccatg
      841 atcatgatta ctgccaatcc cgcatcgcct tcacccacgg aaggcgttaa aacactgggc
      901 caattgatgc ataaccagct gccgatgttt gccacctata aaccgaaacc gaccgacaca
      961 gcggtgatat tttacacctc gggtacgacc ggtcggccta aaggggcaga gctaagccag
     1021 ataaacctat taatgaatgc cgtagtcgtg cgggatctgg ctgcttcagc aatggaactc
     1081 ggtgctgatg cacagaatgt tgttctcatc accttaccgc tttttcatgc caccgctcag
     1141 caagcacaaa tgaacgccaa cttgctagca ggtgcaacct tgacactcat gccgcgtttt
     1201 gagccggggt cggtgctaga ggtgatgaag cgggacaagg ttaatctgtg gaccggcgtt
     1261 ccaaccatgt ttcatgcttt attggactat gcaacagcga acagtatcga tatcgtccct
     1321 gttgcagcca gtttgcggct gatttcttcg ggaggagcgc ctatgccggt cgagttgatg
     1381 aatcgatttc aggatacctt tggcgtccga gttctggaag gttatggact atcggaagct
     1441 tctccggttg tctccttcaa tcatgcagac acgccttcca aagtaggaac ggtgggtcaa
     1501 gcactatggg gggtggatat taggtgcgtc gatgatgaag acgagccggt cccgatagga
     1561 gaaaaaggtg aagtggtcat tcgtggccat aacatcatga aggggtacta taaacaaccc
     1621 gaagctactg cagaggccat gcgcaacggg tggttccata ccggagatat aggcattctc
     1681 gatgcggatg gctatctttc cattgtggat cgaaaaaaag atatgatcat ccgcggaggt
     1741 tataacgtat atccgcgcga gctggaagaa gtcttatcga cgcatcccgc ggtatcagct
     1801 gtagcggttg ttggcattta ccatgaaaaa ctgggtgaag aagtaaaagc ctttgtcgtg
     1861 aaaaaaacgg atagcgaaat taccgaagat gagctgatag cttggtgtaa ggaagaaatt
     1921 gcagcttata aataccctcg ccacattgag tttcgtgatc aattaccaat gaacgccacc
     1981 ggtaaaattc tcaagcgcga attaagacag taatgatatg aagtggagcc taagcttata
     2041 ttcgggctag aaccaataaa aatccggggt gctaaagatt tgatgcgatt taacatcaac
     2101 acaaatatta aattattctt aaaggggttc gtccttaatt aggccgtgca gacgtctgcc
     2161 ccccacacag gctcgcattc accccagatc acttccacca atgaccacta accagactgc
     2221 tggtcagcct cgacctaata ctcaacaagc cttcatgcca aaccggtccg ccacagcgcc
     2281 ggttgacgtc gaagagtggc ccctgtgtga actggaatcc acaatcgaac gtgcatgggc
     2341 cgcaggcgtt agtacatcgc attcccaggc cgtgcgcgac gctgtcgaga ccaccctctc
     2401 gggactcgac tgtggacgca ttcgcgtggc tgagcgccag ggcgtgggcc agtgggccgt
     2461 taatcagtgg gtgaagaagg ccgtgctact gtcgttccgg ctgagtgaca acaagctgtt
     2521 gcgtgccgga gacctgtcgt ttttcgacaa agttgcatcc aagtttgcca acctcaacga
     2581 ggacgatatg catgcgagtg gcgtgcgcgt ggtgccgcca gcggtcgttc ggcgcggcag
     2641 cttcctggct cataacgtcg tcgtgatgcc aagcttcatc aacatcggtg cttacgtcga
     2701 tgaaggcacg atggttgatg gctgggccaa tgtaggttcg tgcgctcaga tcggaaagaa
     2761 tgtacatatc gccgccggcg tctgtattgg cggggtgctc gaacccatgc aggccaaccc
     2821 gacaattatt gaggacgact gcttcatcgg cgccaactca tcagtggtcg agggcgtcat
     2881 cgtcgaggaa aactcagtta tcgccatggg tgtgcacctg ggccagagta ccaaaatcat
     2941 cgaccccgag actaagcaga tcagctacgg gcgcattcca gcaggctcgg tggtcattgg
     3001 gggaacgctc ccgtcgactg atggcagcta tagcactgcc tgtgctgtga ttatcaaaag
     3061 agtcgacgcg caaagccgtg cgaagacgag catcaacgag ctgctgcgca cctgacccaa
     3121 gcagaattca agggcgtcct cgacctacgg aacagatatc tggttccaga ctttggtcgt
     3181 atgggcgctt cgctgcctac agcacggggg cggtacgctt aagccgggca atagtccaca
     3241 agctaattcc cagcgaagca gcattcatcg ccactccacc gatggagccg agcgccaggg
     3301 cattcagcag ccacaagccg gtggatagca taactcgtgc gcgcagacgg gtgccagcca
     3361 ggtaaaaata cgcataggtg gcgttgatgg cagaaatcca gggcagcagt gagaccggac
     3421 ctgcccaggt ctcccacagg acgcccgtaa acaccaggca gtacgaaacg gcggttgcgc
     3481 gccgcccagc tgcagacatc gcaggtgcga ggaacgccag gatctggcga gatgcaatca
     3541 gcagacatat tgcggaggcg gtccacgcta atagcattgc atagtgcaag gcccacagta
     3601 ccaagcccac gcctacgccg gtcttcatca ttttgtcctc acgcatagcg tacgcgccga
     3661 ggctcacgat catggctccg tagcctacca gctgcacgga ggaaaacgga tcaccggtaa
     3721 gagtcacgtt gtagctcctc tagccatggc catcggataa ttgatgagga ttggggggca
     3781 cctgatttgt aagctggagc cttcgaagac acaaggactc aaaaaattta accaaaattt
     3841 taccattccg ttacaacttc ttctttaagt cggattattg ttaccgagcc tcgggaaagg
     3901 ctgaagcttc gctactttta tccagcggga ttgctttaca ggtcagcgga tatagcccgc
     3961 tgcaagaggt gtgaggagag gattcgaatt caatctcaca tttggcccca agctcttaat
     4021 atcaacaaaa ggagataaac atgaaaggta aaaaagtatg ggaacgcggt tcaattttgg
     4081 tttttatatt ttcaggcagt gtagcagctg gagtacctga ggggccattt tcacataatt
     4141 acgatatcgg aaccctgact tcgactccgt atgaaaacgt atttagtggg gagaccaatt
     4201 tactttcaca tagttacaca ttcgatcttg acgccccggc aacggtcgat gcgcgtgcgg
     4261 taaacgctag aatggagaca ggatttatag gtggcctctt ccccgtgact cacacgctgt
     4321 tcgatataag tcttttcgat agcgggaatc acaaattgta tgaaggaaca acaactcaag
     4381 cctggacttt cggaagcacg ttggaagcag ccgtttccgg tgagcttccc gctgggaaag
     4441 attattttgt gcttataacc gggtcccaga caaatgatac tgctctggca tatgcgatca
     4501 agatctcggt tatcccaact accgttcctg aacctgaaac ctatgcaatg tttttggccg
     4561 gcctcgggat gcttgtgtgg cgtatacgac gccaacgaag ttaatcgggc ggatgttgcg
     4621 catgcgaatg tgccggggtg tcgggcgtat ccatcagcag acctttgtgg aaacctattc
     4681 caggtgagct acggccaagc tctacacgac caagacactg actgcaagcg ctatctgctc
     4741 aatgattggg caagttctct tccgggatct cgccttcgat tgaagcgatc cctgtttcga
     4801 cgtataatac gctctcaata aaaacgggac gaggtcactt gtcccgtttt ccatatcttg
     4861 tcctcaatca ggagttcccc gtgtcgcaac acccgcacgc cgtccttgcg ctcgctgacg
     4921 gcacagtatt ccgtggctcg tcggtcggcg ccgaaggggt tactgtgggt gaggtcgtgt
     4981 tcaatacctc gatgacaggc taccaggaaa tactgaccga cccttcctac tgtcagcaga
     5041 tcatcacatt cacctatcct cacatcggca acacgggcgt gaacggagag gatgtcgaat
     5101 ccggcaatat caaccgggtt tacgccgcag ggatggttat tcgcgatctg ccgctggttt
     5161 cgagcagctg gaggagtacc ggagatttgc cgggatattt gaagcgccaa ggcgtgatag
     5221 cgattgcaga tgtcgatacg cgaaagctca cgcgcattct gcgtgaaaag ggtgctcagg
     5281 ccggatgcat agtggcgggc gaagcatttt cccaagcgga tgcagtgcac cgggcgaggc
     5341 agttccctgg actttcgggg atggatctcg ccaaagtggt gagctgcgca gagccttatg
     5401 aatggacgga aggtgaatgg agtttgcggg agggacgcaa gccgaggaag gatacgtctt
     5461 tccacgtcgc agcgctcgat tttgggatca agcagaatat ccttcgaaaa ctagcccaac
     5521 gcggttgtcg agtcatggtt ttaccggcac agacaagtgc cgatgaagtc ctccaattga
     5581 agccggacgg cgtattctta tccaatggac cgggtgatcc cgaaccctgc gattacgcca
     5641 tcactgccac ccgggagatc ctgcaagccg aagtaccgat ttttggggta tgcatgggtc
     5701 atcaattact cggtctggcg attggcgcaa gaaccgtcaa gatgaaattc gggcaccacg
     5761 gcgccaatca tcccgtgcag gacctcgata cagggagagt gctcatcacc agccagaatc
     5821 atggctttgc ggtcgatacg ggctccttgc cagactatgc tcgcgtcacg catgtgtcct
     5881 tgtttgacgg gagcttgcag ggttttgaat tgataggcaa aaatgtcttt tgtttccagg
     5941 ggcatccaga ggcaagcccg ggtccccatg acctcgacta tttgttcgac aggtttgttg
     6001 cctccatgga aaaccgcaag aaggaaggaa tccaaacccg agtgaagtag ctttgccgag
     6061 acgagttctg cccccattta tcggacgttc atggttcgct acccgggtat ccacctatcc
     6121 tcacgagcat ggcacaacaa ttgttcagag cgatatcggc taaagcaagc ctaaactaaa
     6181 taatgcctcg acgtaacgac ataaagagca ttctcatact cggtgcggga ccgatcgtta
     6241 ttggtcaggc gtgcgagttt gattattccg gcgtgcaggc gtgcaaggcg cttcgagagg
     6301 aagggtaccg ggtcatcctg gtgaactcca atccggcgac catcatgacc gaccctgaaa
     6361 tggccgatgc aacgtatatc gagcccatta tctggcccat ggtcgcgaaa atcatcgccg
     6421 cagagcaccc cgacgccctc ttgccgacga tgggaggaca aacagcgctt aactgtgcgc
     6481 tcgatctggc aaaaaacggc gttcttgacc aatacggcgt cgagttgatc ggcgcatcgc
     6541 gcgaggccat tgacaaggct gaagaccgtg aaaagttcaa gcaggccatg actcgaatcg
     6601 gactgggctc cgctcgttcc gccattgccc atagcatcga ggaagccctt caggtacagg
     6661 caatggtggg atatcccgca atcatccgcc cgtccttcac attgggtgga agcggtggcg
     6721 ggattgccta caatcgcgag gagtttctcg acatctgcga gcgcggactg gaagcgtctc
     6781 caaccaacga gttgttgatc gaggaatctg ttatcgggtg gaaagaattc gaaatggagg
     6841 tgatacgcga cagaaaagat aactgcatta tcgtctgcgc gattgaaaac cttgatccga
     6901 tgggcgtgca taccggtgat tccattacag tcgcgccagc tcagacgctg acagacaagg
     6961 aataccagat tttacgggat gcatcgattg cggtcttgcg tgaaatagga gttgagacag
     7021 gcggatccaa cgtccagttt gcaatcaacc cgaaggatgg gcgcatgctc gtcattgaaa
     7081 tgaaccctcg ggtgtctcga tcctccgccc ttgcctcgaa agcgacaggg tttccgattg
     7141 ccaaggtagc ggcaaagctt gccgtgggtt ataccctgga tgagctcagg aatgacatta
     7201 ctggcggcat gatgcctgcc tcattcgagc cggtgatcga ctacgtcgtc acaaaaattc
     7261 cccgttttgc tttcgagaag ttttcccagg cgaacgatcg gctgaccacg cagatgaagt
     7321 cggtcggcga ggtgatggcc atcgggcgga cattccagga gtccctgcaa aaagctttgc
     7381 ggggcctgga aattggtgca gatggtctcg atgaaaaaac cacggatatc gatgtgatca
     7441 ccgctgagct gggtgatccg ggccctgagc gcatatggta tgttgccgat gcatttcgct
     7501 gcggcatgcc gcttgaggaa atacatgagc acacgcacat tgatccctgg ttcctggttc
     7561 agatagaaga tctggtgaaa caggaacagt cggtccttgg aaaagacctg ggttcgctcg
     7621 accggctgga attattcaag ctgaagcgta atggcttttc cgatcagcgg ttggcaaagc
     7681 tgatgcggac aacccagggg gctgtccgct cgcgccgtca tcaactcgag ttgcggcccg
     7741 tatacaagag agtcgataca tgcgctgctg aatttgcgac ccagacggcc tatatgtact
     7801 ccacctatga agaggagtgc gaagcgcttc ccagcgacag gaaaaagatc attgtgctgg
     7861 gtgggggccc caaccgtatc gggcagggca tcgaatttga ttactgctgc gtccatgcag
     7921 cgcttgcctt gcgcgaagat ggcttcgaaa ccatcatggt gaactgcaat ccggagacag
     7981 tctcgaccga ctacgatacc tcggaccggc tttatttcga accgctgaca ctcgaggacg
     8041 tacttgaaat tgtcttcgtc gagaagccct tgggggtaat tgtccaatac ggcggacaga
     8101 cgcctctcaa gcttgcgcga gacctcgagg ccaacggagt tcccatcatt ggcacgagtc
     8161 cggacatgat cgactgtgcc gaagacaggg agcgctttca gaaaatgctg catcagctcg
     8221 gactgaagca gccgccaaac cgtactgcac gaaaccctga ggcggccctg gccgctgccc
     8281 gagagatcgg ctacccgctc gtggtccgcc caagttatgt tctcgggggc cgggcgatgc
     8341 aaatcattca cgaacagacc cacctcgaac ggtatatgca agaggcggtg aaagtttccc
     8401 atgattctcc ggtattgctg gatcgtttcg tgaacgatgc cgtcgaggta gacgtcgatg
     8461 cggtgtgcga tggcgagaca gttctgattg gcggcattat ggaacacatc gaacaggcgg
     8521 gcgtccactc gggtgattca gcctgctcct tgcctccctt cagcctttcg aagccgcttc
     8581 aggacgagtt gcgggggcag acggcagcaa tggcccgagc cctcggagtc gtcgggctta
     8641 tgaatgtcca gtttgccatt cagggtgaaa ccgtcttcgt gcttgaggtg aatccgaggg
     8701 cttcccggac cgtacccttc gtatccaagg ccacggggct gccactggca aaaatatccg
     8761 cccgctgcat ggccggtatc tcgctcgttc gccagggcgt agtgaaggaa ataatcccat
     8821 cctattattc cgttaaggag gcggtcttcc cattcatcaa gtttccgggc gtagacccta
     8881 tactcgggcc agaaatgaaa tccacgggcg aagtgatggg tgttggtcaa acctttgcgg
     8941 aagcattcgt caagtcgcaa ctcggcgcgg gggtgaaatt acctcgcaag ggcaaggtat
     9001 tcataagcgt gcggcaggtc gacaagccgc gtgcggtcga gatagcgcgc aatcttgcac
     9061 aacttggatt cacgctttat gctacacgcg gaaccgccac tgtgctgagc agcgcgggct
     9121 tgaccgtcac tcccgtgaac aaggtcgcgg aaggacgccc gcacatcgtg gatatgatca
     9181 agaatggcga aatggacttg atcatcaata cggtggaaga caaacgcagc gccgtgcatg
     9241 actcgtattc gatccgcaac gctgtattgc aggccagggt aacgtattac accacgcttg
     9301 caggtgctcg ggcggcctgc atcgggatgg cgaatatggg cgaattgcgg gcatacagtc
     9361 tgcaggaact ccagggatcg gcggcagagg caccgtgcga agcgtctgat gagggacatc
     9421 tcctggtaaa acaattttga tagatggcag caacaggact atgggtaaaa ttccattaac
     9481 agtaattggt gcagaaaaac ttcgcgcgga attgcatgaa ctcaagacgg tgcagcgtcc
     9541 cgctgtgatt acggcaattg ccgaagcacg ttctcatggc gacctttcgg aaaatgcaga
     9601 gtatgaagcg gcgaaggaac ggcagggttt tatcgagggg cgtattgcag agctggaaac
     9661 taaactttcc aatgcccaga ttatagaccc ggcgttgctc gacgctgggg gggcctgcgt
     9721 atttggcgct acgatcgacc ttgaagacat gaccaattca accacggtaa cctatcagat
     9781 cgtgggagac gacgaggctg acatcaaggc ggggaagata tccgtcagct cccccatttc
     9841 acgggcgctt ataggtaaat acccaggaga tgtggcggag gttatagccc cgggtggtgt
     9901 tcgagagtac gaaattctgg atgtgaagta cgtttagtgg gagcgcctca atctctcaag
     9961 aactgaaatc ctgaagacaa cgagttcgct tccgtacttc atcgcccgcg tcttggagcc
    10021 atctttctct tagggcggag agacggatcc ttgttcgcgg aagcgatcag ctccctgctc
    10081 tttcccttgg aagttgcgcc gcttgttgtt ttccggatat tttcaacggg tatctcattg
    10141 ggaaccgggc ggtagattac gaacatcctg cctatgtggt ggatcggagc agctctgagc
    10201 cgcccgcaaa tctgttccag caatgcctct cgcgtttccc gctcatcgtt tgaaaccttg
    10261 attttgatca gctcgtggct tttgagagcc tgatccagtt cacgaataac ggcttccgac
    10321 aaacccccgc cgccgatcca tacaacaggc ttaagggaat gggaaagggc tttgagggtg
    10381 gctcgatggc ttgaagagag ttccagcatg agatcaaatc aatccaggac gatatcttgc
    10441 cattttaagc aatgaaacgc accaagacca gcaaggcctg gatgaaagaa cacgtaaacg
    10501 attttttcgt caagcaggca aaaaaggagg gctaccgctc ccgtgccgcc tacaagctgg
    10561 tcgaaattgc ggagcgggat cgtttgattg caccgggcat gacaattatc gatctcggtg
    10621 cggcaccagg gggatggtcc caggtcgcag ccgaaaagtt ggcggggacg ggaatggtga
    10681 ttgctgtcga tctgcttgaa atgcaagcga tccccggcgt cctgttcatg caaggggatt
    10741 tcagggagcc gcaggttctg gcggaagtga aaaaaaagct ggagggccga cctcccggcc
    10801 ttgtaatttc ggacatgtcg cctaatataa caggcataag catagtggat caggcgaggg
    10861 gaatggagct ggctgagctg gctctcgatt tctccgtcga tcaattgaac tctggtggaa
    10921 attttctcgt caaagttttt cagggcagcg gtttcgaggc atttttaaat gcaatgcgca
    10981 ctacgtttga tcgagtggta acacgcaaac ctggagcatc ccgcgatcga agcagcgaga
    11041 tatatttgct gggactgggg aagcgaggct gattttcacc ggagtggctt caataaaacg
    11101 ggagccagcg accagcgtgc tactataaat ctcgaaatgg gtttagaatg aagcattcaa
    11161 ggatattgac ggcgcaagcc aaggagctat cttgaacaat ctcatcaaaa acatggccat
    11221 ctggctcgtg attgctttgg tgctcatgac ggtattcaac cagttcagca cccggcaatc
    11281 ggcgcaggcc cccatggaat attcgcagtt catcgaagag gtcaagcagg gcagaatcgc
    11341 caaggttacc atcgaagggc gtaccataaa aggcacgaaa tccgatggaa ggcgcttcac
    11401 gacctatacc ccttcagatc catggatggt gagcgacttg ctcaaggcgg gggtcatcat
    11461 agacgccaaa ccggaggaag agccttctct cctgatgaat atttttgttt cctggttccc
    11521 aatgctgctg ctgatcggcg tgtggatttt tttcatgcgt cagatgcagg gaggcggacg
    11581 gggtggcggc gccttttcgt tcggcaagag caaggcgcgg atgctggatg aatccaccaa
    11641 ccaagtgacc tttgccgatg ttgccggttg cgaagaagcg aaggaagagg tctccgaact
    11701 ggttgaattc ctgcgcgacc cgagcaagtt ccagaagctc ggcgggcgca ttccccgcgg
    11761 cgtattgatg gtgggcaacc cgggaacggg taagacgctt ctggcccggg ccattgccgg
    11821 cgaagcaaaa gtccctttct tcagcatttc cggttcggat tttgtggaga tgtttgttgg
    11881 ggtgggcgcc gcacgtgtac gggacatgtt cgagcaagcg aagaagcatg ctccctgcat
    11941 catctttatt gatgaaattg atgcagtcgg acgtcagcgt ggcgcaggtc ttggaggtgg
    12001 caacgatgag cgtgagcaaa cgctgaacca gttgctcgtg gaaatggacg gctttgaggg
    12061 aactgccgga gtcatcgtga ttgcggccac caaccgccct gacgtgcttg atccggccct
    12121 cttgaggccg ggccgcttcg accggcaggt aacggtgccg ctccccgata tccggggacg
    12181 cgagcagatc ctccacgtcc atatgcggaa ggtgcccatt gcaccagacg tccatgcgga
    12241 aattattgcg cggggaacgc cggggatgtc gggtgcggat ctcgctaacc tggttaacga
    12301 ggcggccctg tttgctgcac gcagcaacaa gcgtctcgtg gatatggatg actttgagcg
    12361 cgcaaaggac aagatattca tgggtgcgga gcgtcgctcc gtcgtgatgc cggagcgcga
    12421 acggcgcaac actgcttatc atgagtccgg gcacgccgta gtggctcagc tgttacccaa
    12481 aacagatccc gtgcacaagg tatcgatcat tccacgggga cgggcgctgg gcgtaacgat
    12541 gcaattgcct accgaggatc gtttcagcat ggatcgggag gaaatcctgc agagtatcgc
    12601 ggtcctgttt ggaggtcgca tcgctgaaga gattttcatg gggcagatga ccacgggagc
    12661 ttcgaacgat ttcgagcgtg ctactgagat ggccaggcgg atggtgaccc aatggggcat
    12721 gtctgaccag cttgggccaa tggtatatgg agagaacgag ggagaggtat tcctgggtcg
    12781 ttccgtaacg acacacaaaa atgtcagcga agcaaccatg caaaaggtcg acatcgaaat
    12841 tcgccggatc atcgatgcgc agtatggctt ggcgcgcaag ctgattgaag aaaaccggga
    12901 caagatcgaa gtcatggccc aggctttgct cgagtgggaa accatagatt ccgaccagat
    12961 cggcgacatc atggcagggc ggactccccg ccccccgcgg cctacaaagg tgacctcgac
    13021 gcctcccaag gacaacacgc ctcctggggc gcctgcagca acagcaactc ctgcccagga
    13081 ggtttaaatc cgaggcagga agcgttaaga acagtcacga atgacaaggt ggggcttgcc
    13141 ccgccttttt tttgtacctt gataccttcc catcttcctt tcttccttgc ctggatttga
    13201 ctctaatatt tcccccgtcg gtcgtcccct catcatgggg attatcaacg taactcccga
    13261 ttctttttct gacggtgggc tgtatgcttc acttgggcaa gcgctccgcc acgctgagct
    13321 gctttttgaa gagggtgctg acctgctgga tgtgggaggg gaatcgacgc gtcccggcag
    13381 tattccggtt agcgtggatg aggaattgcg gcgaatcatg cccgtggtgg atgggttggt
    13441 gcgcatgaat gtacccgtat cggtcgatac caccaagcct gaggtgatgc gggccgcgat
    13501 acgcgccggc gcagtaatga tcaacgacgt gaatgcgttg cgagccgaag gagcccttga
    13561 agcggctgcg tcgggagatg tcgcagtctg cctgatgcat atgcagggcg agccgcgctc
    13621 gatgcaagtt cagccacgct acaatgatgt ggtgaaggag gtaaaggatt ttttgcgtca
    13681 acgactggat gcagcgcgtg cgtcaggtat tccggacaag cgcttggtgg tcgatccggg
    13741 ttttggtttc ggcaagacgc tggagcataa cctcgagctg ttgcgcaatc tggatcaatt
    13801 cgccgatctt ggcgttacgc tgatggcggg actatcgcga aaatcaatgc tgggaaaaat
    13861 aaccggaaac gatgtggctg cccgggttca tgccagtatc gccgcggcac tgctcgcagt
    13921 tgcgaaaggt gcccgaattg tccgggtgca tgatgtaaaa gcgaccaggg aagctctcgc
    13981 catttataat gcggttaaca acccggtacc gggtgaagaa gggtggtctt aaaaagtctg
    14041 cccaggaggg taatgtgggg gtgattcaag cccgtcgctg gaacaccctt ttgcccgctt
    14101 ggactctatt tcagggagtg attggaattg actagaaaat tttttggtac cgacggaatc
    14161 cgcgggcgtg taggagaagt ccctatcacg cccgagttcg tcatgcacct cggctactcg
    14221 gccgggaagg ttctggcttc tgcaaactgg catcttgccc agggcgagcg tccggctgtg
    14281 ctgatcggga aagatacgcg catttccgga tacatgctgg aatcggctct gcaagccggg
    14341 ttatcggcgg ccggcgtgga cgtgctgctt tcagggccaa cccctacccc ggcggttgct
    14401 tacctcacgc gcgcattgcg gctgcaggca ggcatcgtca tctccgcgtc gcacaatccg
    14461 ttcgatgata acggaatcaa gttcttttcc gcctcaggca ccaagctccc cgattccgtg
    14521 gagcatgaga tagaggccgg tctgaatcag ccgataaaat ccatgccatc tgcacagctg
    14581 ggcaaagcgc gccgtgtcaa cgacgcgcgc ggacgctata tcgagttttg caagagtacc
    14641 tttcccaacc atctcgattt gcgaggcttc aggatcgtgg ttgactgtgc gcatggggcg
    14701 acataccaga ttgcaggaca cgtgcttcac gaactgggag cggaagtggt cgctatcggg
    14761 gctcagccgg acggcctcaa tatcaaccac gagtgcggcg cgacacacag cgctacgata
    14821 ggcaatgcgg tcaagcaata caaagctgat attggcatcg cgctggatgg cgatggcgat
    14881 cgcgtgataa tggcggacaa taacggcgtt ctttacgatg gcgaccaact gatctacatc
    14941 atcgctgtgc accgcaagcg aaatggcctg ctcaggggcg gcgtcgccgg aacattaatg
    15001 acgaatctcg cggttgagaa tggcttgaag aaattgaacg ttccctttgc ccgcgccaac
    15061 gtcggtgatc gctacgtgct tgagttgatg cagaagaacg cctggcaact ggggggagaa
    15121 ggctcggggc atatcatttg cactgacaag catacgacgg gtgacggaat catttccgcg
    15181 ctgcaagtcc tttatgcggt tcgcgattct tcgagcacgc tcgccgagct tgcgcgagac
    15241 gtcacgcttt atcctcagca gctcataaac gtgaaagtgc cgaaggggtt tgattcgggc
    15301 aagagcgccg tcatcaaaaa cgtgcaggct gaagcggagc aggacctgaa cggaagtggc
    15361 agggttttgc tgcgtgcatc aggtacagaa cccctgatac gggtgatggt ggaggggcag
    15421 tcttcgcaga aggtgagtca ctgggcggaa aaaatagcgg atgccgttcg gactgccgca
    15481 gcatcgtgat agccggggtt cggcatcagg gtggagtggg caggactgag atagctcacc
    15541 tggattgaat ctggcttcac aaaacggtaa taccggtgga ctaacatagg cacttttaat
    15601 cgatcaacaa taccggggaa acgagatgtt gaaattgccg agatggatgc tgggaatatc
    15661 cgttgcagca gccgctttga ttgctggcat cagtatggcg gcttcgggca ggatggtaaa
    15721 aattgatggc tccagcacgg ttttccctat taccgaagcc gtggcggaag acttccagaa
    15781 tgcgaagaga ggtgcggtaa gggtgaccgt cggcatttct ggcacgggcg ggggattcaa
    15841 aaagttttgc cggggggaaa ttgacatcgt ggatgcatct cggcccatcc tggaaaagga
    15901 aatggccgac tgtagggccg caggggtgca gtacgtcgaa atgccggtcg cgtttgatgc
    15961 cctgaccgtc gtggtcaacc cgaaaaatac ctggagcaat gttattaccg tggcagagct
    16021 gaaaaaacta tgggagcctg ctgcgcaacg aactatcatg cgctggaacc aggtaaaccc
    16081 cgcatggccg gacgagccaa taaagcttta tggagcgggc gcggactcgg gcacctttga
    16141 ttattttacg gaagcaatcg tcggcaaggc aaaatcgagc cggggagatt ttacggcatc
    16201 tgaagatgac aacgtgctgg tacagggagt tgccagcgac aagtacggac ttggtttttt
    16261 tggttttgcc tattacattg aaaaccagaa aaagctgaag gcgatcgctg tcgacgcagg
    16321 gaagggacct gtgatgccct ccgccaggac ggtggaagat ggcacttacc agccactgtc
    16381 ccgccccatc tttatttatg tgaacgtcaa gtcagcggaa agacctgaag ttcgggagct
    16441 tgtggagttt tacatgaaaa acggccccga actggtgaag gaagtgaagt ttttcccatt
    16501 gccttcaaag gtttatacga ccaatctgga acacctcgat aagaagaaag tgggaacggt
    16561 atttggtggc aaggcagaag tgggcctcag gattgaagaa gtcctcaagc gcgaagccaa
    16621 actctgagcg tatcctctat aaacccgctt cacatgtcga gcacatgaag cggcagggtg
    16681 tcggcctaaa agatgcggat gttgcataat tcgaggtttt cagccgcgag acgatcaaac
    16741 tgccaggcgc agctctatcc cagcgttata atagcggctg cgtccatttc tcgtgcagct
    16801 catgagcccc ccaggctacc caaactttaa attccacttt accgaactcc tccgtcagct
    16861 cctaagcggc atgttgccca aggggctgga gcctgatatc gagtttgcgc ggccgaagca
    16921 ggcaaaccac ggggattatt cctgcaacat tgccatgcag ttggccaagc cgctgcggcg
    16981 cagtccccga gatatcgcga ccctgctcgc cgaagggctc gctgcttcgc cttatctcga
    17041 aaaagttgaa gtcgcgggtg gcggattcat caacctgttc ctcacgctgt ctgccaagca
    17101 gcagttcccg aactatgtgc tgtcggaggg tgaggggtac ggacgcagca atatcggtgc
    17161 ttcccggaag atccaggtgg agttcgtctc ggctaatccg accgggccac ttcacgtagg
    17221 tcacgggcga ggtgccgcat ttggcgccag tcttgcgaac gtgctggaag ctgcgggtta
    17281 ttccgtcacc cgggagtatt atgtgaacga cgcgggacgc cagatggata tcctggctct
    17341 gtcaacctgg ttgcgctatc ttcaactgaa cggcctcgcc atttcatttc cccctaatgc
    17401 ctaccagggc gagtatgtgc gggatatggc ccggcttata taccaagccc atgccgcccg
    17461 ttatgtccat gaacccgaat tgcttttcga ggggatcggt cacgaagcgg agggcgaggc
    17521 gcgtctggat caccttatcg ccaatgcaaa aaaattgctg ggcgaggact atgcgtatat
    17581 tcacaatttt gttttggcgg agcagttggg agattgccgc aatgatctga tggaattcgg
    17641 ggcagttttc gacgtctggt tttcagagca atctttattt gatagcggaa gtgtgtctcg
    17701 cgcaatcgat ttcctcgatc aaagcaatca catataccag caagacgggg caaaatggtt
    17761 taaatcgacc gatttcggcg atgagaaaga cagggtcgtc cagcgggaga acggacagtt
    17821 cacctatttc gcctccgata ttgcctatca tctcaacaag ttcgaacgcg gtttcgagcg
    17881 cgtcatcgat atctggggag cagaccacca tggatacatc ccccgggtaa aaggggcgat
    17941 gaaggccttg tcgctcgacc cggaaaaact ggaaattgca ctggtacaat tcgccattct
    18001 ttaccgcgac ggaaaaaaag catcaatgtc aactcgttcc ggcgaatttg tcacactgcg
    18061 ggagttgcga caggaggtag ggaacgatgc tgcccgtttt ttttacgtcc ttcgcaagag
    18121 cgatcagcac ctggactttg atctcgatct tgcaaagtcg cagagcaccg ataatccggt
    18181 ctattatgtc cagtatgccc acgcaaggat ttgcagcgtg cttgaacaat gggccggaga
    18241 cgcttccgtt ctcgcaagag cggacatgtc gccgcttacc ggcgcggcgg aactttcgct
    18301 gctgcaaaaa ctgatcgatt atccggaact gttggaatcg gcagcccggg aactcgcgcc
    18361 gcacttggtt gctttttatc ttcgggaact ggcgggcgaa tttcacagtt actacaattc
    18421 cacacgcttc ctggtaccgg aggttccggt gaggctggcg cggctcgggt tagctgccgc
    18481 tctgcggcag gtgttgggta acggcctgaa actgctgggt gtcagcgctc cagctaaaat
    18541 gtagattgtc ccatccgcag gttttacatg agccgcgatt acaaaacaag ccctaattct
    18601 ttgaatccct tcaaaggcag aaattcgctg gtcatcggca tattcatcgg ctatgcgctc
    18661 ggtctgctga ctgctatcgc gacatggatg tacatcaaca aagggccgag cccctttatt
    18721 tcggcagaca aacctgccga tagcgcgacg gcaaaaagcg gtgcaggtat accgcaaaaa
    18781 ggcgctcaag gcctggcctc accggataag agcacggacg gaaagccgcg cttcgagttc
    18841 tacaacatgc tgccgaatga gggggaaacg atcacggagc agcagctcaa acaaggggct
    18901 cagcagccgg cctccaagga caagtatttc cttcaagccg gatctttcca gaatgcaagc
    18961 gacgccgaca acctgaaagc caagcttgcg atgctcgggg tggaggcaac ggtgcaagcc
    19021 gtaaatgttc ctgaaaaaaa gggtacttgg catcgggtac gggtagggcc attctcaagc
    19081 attgacgacc tgaaccaggt gcgcgcgtca ttgcagcaaa acggggttca aagcagtgtt
    19141 atcaaggttc aggacggaaa gcagtagctc gcacatctta accagatgcg tacgtatccc
    19201 ttattgtatt tcttcatgaa gaaggagaaa aaatgaagcg catgcgttta ttcgcgttga
    19261 tcctgttttt cgccgggacg acctttgcca ccgctttcac cgctcacgcc gagcttgtcg
    19321 aggggcgcga ctacactgtt cttcgtaacc cgcagcctac tcacagcgat aacaatgtcg
    19381 aggttataga gtttttctgg tacggctgtc cccattgcta ccagctccat ccctacatta
    19441 gcgagtggct gaaacggaag ccgaaggatg taagtttccg gtacgttccc gccattttcc
    19501 gcccctcatg ggttccggcg gccaggattt tctatgcact ggaggttctg ggtgcaaggg
    19561 acaagctcca tgacaaggtt tacgatgcca tccacgttga aaagattgac ctgaacaagg
    19621 aagatgtttt gttcgattgg gtggcgaagc agggaataga tcgtcagaaa tttatcgacg
    19681 cctataactc gttctcggtg cagaaccagg atataccgcg ctcaaccaac atgtcgaaaa
    19741 gctacgggtt gacaggagtt cccgcggtgg tcgtggatgg caaatacatg accagcggga
    19801 gactgggggg cacgcccgag gacacgatac ggataatgga ggaattgatc gaaaaggcac
    19861 gggccgagag ggtaaagaaa taggaaatgc atgcaatagg aaatgcatgc aagggtgccg
    19921 attaatttaa atcggatcgg atcaagtccg gatgacaagc ttgtagtatt ttcagcctgg
    19981 tgatcagaaa atataagagg agctgaaacg atggcaaccg aaaaagcgat tttcggcgcg
    20041 ggatgcttct ggggggtgga ggcagcattt cgctcagtaa agggcgtgcg tgaagcaata
    20101 tcaggatacg ctggcggcca tacgccaaat ccaacctacc ccgaggtctg cacgggagaa
    20161 acggggcacg cggaagcggt gcaggtggag tatgatccgt cgcaggtgag ttacgaccgt
    20221 ttactcgacg tattctggaa ctgtcataat cctaccactc gcaatcgcca ggggccggac
    20281 atcggtaccc aatatcgctc cgcaatattt ttttatacgc ctgagcagga ggaggccgca
    20341 aaagcatcaa aggaaatgtt acagcgcggc ggccgttggc aagctcctgt tgtcaccgag
    20401 attttgcctg ccctcgagtt ttatccggcc gaggaatacc accagcgtta ttttgaaaag
    20461 catggtattg cgggacattg ccatacctga gcgatacctc gcgaacaagt ctgaaaatta
    20521 aacgttgccc gtgcaaggcc ggacaggacg agcaagacca agaggtgagg cgaaagtatc
    20581 gctttttccg tgtggagaga atagcgttcg cttacaatct tcccagccta ctctaccgcc
    20641 tgttgatcga gcattgatag gcggtttcgg atcgcagcaa tacgcgcttc gctgaccttg
    20701 gcgttaataa cgtctggcaa ccggctggct tctgatactg ttccggccgt tgcagccgcg
    20761 attgcccccg catccaccga tcttgctgcc agtaacgcct cactcaaccg ttgggcttgc
    20821 ggataggatt tttcggcata cccaggccgc ccgtgaaagt cgcatgagca ggcctgcaga
    20881 aactcctcga aacgctcagg cttgcggtaa gcatccaccc cttgcagtat gcgtgcgatg
    20941 gtagatgccc ggagttcgag cgcccggtga acgtccccgt gatagcgggc gacaagcagg
    21001 gccaggtcgc gcgcctcatt gggtaccctg atgcgttcac ataagccgcg caccagcctc
    21061 acgctacggg cctcgtgccc ataatgatgc ggccattcct caagaggcgt tgtgcctttg
    21121 ccgagatcat gtgtcagcgc cgcaaaccgt accggaagcg agtaattttt tgcggctgcg
    21181 tggtcgatta ccatcatcac atgaacgccc gtatcgactt cagggtgata tttaatgggt
    21241 tgggggactc cgaaaagcgc gtccacctcg ggcatgatgc ggattagcgc gttgcactcg
    21301 cgaagcgcat aaaacatgcg cgaaggcttt tcttccatca accctcgcgc aaattcctgc
    21361 cagacccttt ccggcaccag cgaatcaatc tctccgttat gtaccatttc attcattaac
    21421 gctagagttt ctggcgcgat acgaaacccg aagcgtgcgg cgaagcgtgc agtacgcaag
    21481 acacgtacgg gatcttcgtt gaatgccggg ctgatgtgac gaagtacgcc tgcttcgagg
    21541 tcggctattc cagcaaatgg atcgatgatt acgccactct ggtccttggc tattgcattg
    21601 atcgtcagat cacggcgggc aagatcttcc tgcagtgtga cttcagggga tgcataaact
    21661 tcaaacccct tataaccgcg cgaaatcttg cgctcagtgc gagccaaagc atactgctcc
    21721 tggctttccg gatggaggaa tacgggaaag tcttttccta cggggcgata ccccagactc
    21781 aacatctctt ccggcgttga tccgaccacc acgtagtcat ggtcctggac gggcagaccg
    21841 agaatctcgt cgcgcacaga accgccaacc aagtaggttt tcatcaaatc aacccgatcc
    21901 caggcttgct gtatcaccgc cctgcgcgct ggcgaaactc atcatagcgt tcgcggggca
    21961 gttcgcctgt cagataaaac ggcgctactt cttccatggc aacaggatga atggcccaca
    22021 cgtcctctaa aacgctgtag ccctcacagt cgcatatgtt gtcgattttc atcgaaaagt
    22081 aattgtcccg cgtcataagc ttgccgggca ggaattcgag caatagcgcc tcgagatagg
    22141 agaagctgct gttgaggcct atgattgtcg gctcaccgcc ggtggcctgg gcagcgtact
    22201 cgaccatctg ccggaggcta tagcattttg ggccgcagag gtcatagctc ttgccgatag
    22261 tatggggagc gtccaggctg gcagcaaagg cgcgcaccac atcttccacg aataccggtt
    22321 gaaatttggc agccggcgag gccaaagcca atatcgggaa ccttcccaat cgggcaaaaa
    22381 gcgtcaatga tgaatcacct gggccaaaaa taaccgaggg ccggaaaacc gtggtttcca
    22441 ggccggatga tttcacgatg cgttcgccct ctcccttcgt tcgaaggtaa gcgctgggcc
    22501 gtccctcgcc cgcattcaaa gcgctcatgt gcagcaggcg ccggatacca ttgcgttgac
    22561 acgcagctac gattttcctc ggcagttcga cgtgcgcggc ctggaaatcg ccgtgaatca
    22621 cacccaccag gttgatgacg gcgtccatgc cagccagcaa cctgtcaagc gtggaatcgt
    22681 catatacgtc agcctgtacg acttccacgg taggcagaac cagaagttct ttggaacgct
    22741 cgcgactgcg tgcagggatg cgcagaagga tttcctccgc cgtcaaaaaa ttggcaagat
    22801 gcccgccaac aaaacccgtc ccgccaaaaa cacaaacact attgaccgcc acggctcatc
    22861 cctccgccct actggaattt ttccaggctc acccgtggcg gtacaattcc caaccgctgc
    22921 ttcaaggggg gcaactgatg gccaaacgtg cgcgcgtaat aggtcgagtt actcatgact
    22981 ttcttgacat aatcgcgcgt ttcgctgaag ggaatgcttt ccgcgtaaat cgccccttcc
    23041 agcggcttat catcgcgcca ttgcaatgcc cggctggggc ccgcgttata ggcggccgaa
    23101 gccagcaggg gatggttgtc gaacagatcc agcacgtgct tgaggtaata tgttcccagc
    23161 ttcaggttgg tgttcacctc agaaagcgtt tttggatgtt tcctcatccc catccttttc
    23221 gcagcccatt ttgctgtcgc gggcatcaat tgcatcaggc ccattgcgcc tgcgctggag
    23281 cgggcgtccg tcacgaaccg gctttcctgc cggataaggc cgtataccca cgcttcgtcc
    23341 agttcctgtt gcttcaggat ggcgcgcagg gtgtcgcggt ggggagctag aaagcgcaaa
    23401 ctgaagtcat ggttctgcgt cgtcttatcc gccgtatgaa tggcccgatc ataaattcca
    23461 ttttgccgcg cgacttccgc agcggccaga agccggcgat catcgaaatc acggatggcc
    23521 cagccccact ctcgcgacgc ttcggtgcgc aaattcagac gatacagggc aagcgcgcgc
    23581 cgtataccgg ggagccgctc gatcctggaa acatcctccc gagtaatctt gaggggctca
    23641 atgggggctt ggagggtcaa acccagctcc tcttccgcca gttggccgta aaaatgatgc
    23701 tcgacgctga ggggtgcgaa aatggcgttc gcctcaatta ccttgttgtc ggctttgagc
    23761 gcccgcgctt tccagtagcg ccagactgct gttccctgct cctcctccga cattgcttga
    23821 atagtatcga gtaccagcgc ccagttgccc gcacgcaacg ccgagcgcac tttccatccc
    23881 agttgcgcat cggacaatgg tgccggttga gtgctcctgc ctgcttccgc aaaccactcc
    23941 agcgcacgag gattgagcct tcgcgccgcc tggtccgcaa gttgcaccag gaaataggaa
    24001 cgttctgcct cattgaattg ctcacgaacc ctgggccagt atacatacgc ctgatctgga
    24061 ctgattcggc tcatccgcag cattgcaaac agcgcaattt cgcggtcagc gcgcctcgcc
    24121 atctcattcc ggtgattttc caggtagcgt agagggccct cctgggcgtt cgatagcctg
    24181 ccttcgtcaa gcgcctcggt atcaggcaag tactcgttga tacgtttggc gaccccaacg
    24241 tttcccgctt ccagtgccag gcgcagcctg gcccatatat cttcgaccgt cagcacccct
    24301 gcattcacga gcgtatcaaa tagaggagcg cagctttccg gcaaatccct ggggttgaac
    24361 cacagcggtc gggcttcggc aagtgcgatg gcatcgccgg cggccacgcg gtgccgatag
    24421 gaataacagg caagctccgt gtcgaggctg gtaacgcctg cgtatgtttc cccgaacaga
    24481 tcccactgtt catttcttcc cagaattttc acccactcga gcagcaaccg gtcggcaaca
    24541 ggagtatcct gatatcgaag aagaaaattt tttacggagt caggacttag gacagaatcc
    24601 tccagttgcg ggcgcaactg ataataggcg gcgtatggct ccagcatatg cccttgcagc
    24661 cgctgggcat aaaatgaaac gcgggcacga tcgcccttct gaaaagcctg cctcatggac
    24721 atgaaatcgt catccgtggc ggcgtgcaaa gctccggcat acaatacgag gcttaaccct
    24781 gcgacaaact ttttcattac ctgctatacc taaacaaaat gtttaatgca aaatccatcg
    24841 ggagtgcttc gagcgcttca ggcagttttc gaagcggcgt tttcgaagcg atgttttaag
    24901 tgaagaatat cacagatgcc ttatatcagc ttaaacccgg caaccaataa aacgatcaaa
    24961 acatttacca gctgggacag ccggcgcctc gcggcggctc tgaaagaggc tgataacgcc
    25021 gggcgcctgt gggcggagag atctttcggt gagcgtgcgg agtgcatgca tcgggcggcc
    25081 accctattcc acgagcgggt tgaaacatac gcccagttga tggccgtgga gatgggcaag
    25141 ccgctgcgcg agggccgcgc cgaagtggag aaatgcgcgc tcacttgtga ctattacgcc
    25201 gttcacgctg aacatttcat gcagaccgag ctggtgcaga ccgatgcaag caagagcttt
    25261 gtctgttacg ccccgttagg cgtgctactc gcggtaatgc cgtggaattt cccgttcttc
    25321 caggtgatcc gttttagcgc cccggcgctg atggcaggaa acgcttgtgt cctgaaacac
    25381 gcatcgaacg tgccgcaatg cgcgctcgca ctggagcagt tgtttcagga cgccggattt
    25441 cctcctcatc tgtttacgac gctcatgatc gaagcgtcag acgtcgccga cgcaatcgcc
    25501 agccggcatg ttcacgcggt gacccttacc ggctccgaag ccgcggggcg tgaggtcgca
    25561 tctcattccg ggcagcattt gaaaaagtgt gtgctggaac tcggtggctc ggatgccttt
    25621 gttgttctca aggatgctga cttggacctg gcggcgacct ttgccgtgaa gtcacggttc
    25681 cagaactgcg gacaatcgtg tatcgctgcc aagcgactca ttgttgtgcc ggagattgcg
    25741 gacgagtttg tgagcgtgtt catcgagaaa gccggggatc tgaaaatggg tgatccattg
    25801 aacgaagcca cacaaatagg cccgatggct cgtcttaatc tgcgtgatgg cttgcatcaa
    25861 caggttatgg attcgatagc gcagggcgct cagccggttc tcgggtgtca gctgccaccg
    25921 ggggaaggat cattttatcc cgtgtccgtg ctcgatcatg ttactgcgag aactcgggct
    25981 tatcatgaag agttgtttgg ccccgttgct gccgtgattc gcgtgggaaa cgaggaagaa
    26041 gcgatcagta ttgcaaacga cagccgctat ggcctcggtt cgagtatctg gagccgcgat
    26101 atcgagcggg ctcaaaaact gtcagaagaa attgaggcag gctgctgttt tgtgaatggg
    26161 atggtaaggt cagaccctcg tctgcctttt ggcggcgtca aggcttccgg tttcggcagg
    26221 gagctttcct atcacggcat acgcgagttc gtgaatatca agacaatttg ggtgcgctaa
    26281 ccctggccgg ggattcagca gaatctctag caggattgat tccaaatccg agcaaaataa
    26341 ctggctttgg ctgcactcgt tcaccggttg gtttcgaggg ttttcgcgga gacaccagtg
    26401 aaaat
//
LOCUS       DASSHJ010000032         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_631727, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000032 DASSHJ010000000
VERSION     DASSHJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631727"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="VFI43_01605"
     CDS             complement(<1..1789)
                     /locus_tag="VFI43_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7060854.1"
                     /translation="MVYIGDEVVEAGGLSHNAHDVLAPFGAPISLAAHMKRYTIEQFM
                     NSTSILGASFSADERSILFSSNASGVFNVYTLAIPKGEKGCGIGEPQPLTRSTTDSTF
                     AVSFFPSDNRILFTRDRGGNENFHLFVLGEDGNEMDLTPGENLKAQFIGWRADGSAFF
                     VSSNERDARFFDLYRYDTRNYTRTLLYKNEQGFDLGPISRDERWIALNRSNTTSDGDI
                     YLFDVEKQQTRHLTEHEGSIGFHAETFDPASEKLFFLTNQGSEFKRVCTYDLAGGGIE
                     EKESADWDVISTYFSRNGRFRVTAINQDGSIVIRVVEIVDEDGRKREIPLAIPRLPEG
                     EIRGVVFSESGNFMAFYVNGDRSPDNLFVQDLHTGSLQQLTCSLSPDIDPMDLVEGQV
                     VRFKSFDGLVIPSIFYKPQGASSSNKVPALVYVHGGPGGQTMHGYSAQIQYLVNHGYA
                     VLGINNRGSSGYGKTFFTAANRKHGREPLWDCVEAKTYLASLGYIDPERIGIMGASYG
                     GYMTLSALAFRPEVFKVGVDIFGVSNWLRTLESIPPYWESVRKAIYDEIGDPVRDIDI
                     LVATSPLFHAKEIRKPLMVIQGANDARVVK"
     gene            1953..2498
                     /locus_tag="VFI43_01610"
     CDS             1953..2498
                     /locus_tag="VFI43_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7060855.1"
                     /translation="MFKKIVTLAALAAGGLMLTKYFRKNHGSEKMSTVEESIEVNVPV
                     STAYNQWTQFTDFPKFMKGVQEVRQIDPTHLHWRAIVGGKEEEWDSEITEQIPDTRIA
                     WRSTTGPKNCGVVTFHKISDSTSRIMLQMDYAPQNVAEGIGDMLGIVKAEIHNNLKSF
                     KEFLEERGKETGAWRGAVPQQ"
     gene            2670..3254
                     /locus_tag="VFI43_01615"
     CDS             2670..3254
                     /locus_tag="VFI43_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7060856.1"
                     /translation="MTGDPTLLILLYFILPVWLLAGFADWLCHRASHIATTTGAKESL
                     LHLLMFAEIGLPLLAAIFLQINAGIIALMIVAFFIHQATALWDVSYAVTARNVSPIEQ
                     HVHSFLEMIPLMAILFVVTRHWDQFLALFGFCAEPPRFSLAWKEEPLPATYVISIMAA
                     TLLFEIIPYVEELARGLRANHGMLVPPKARSKNE"
     gene            complement(3525..>4566)
                     /locus_tag="VFI43_01620"
     CDS             complement(3525..>4566)
                     /locus_tag="VFI43_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7060857.1"
                     /translation="ARSRTSWGAFHPEAEEEVRSTLCQKQFIAAAGGLDAAMMVAPCT
                     AYGSRKHPTLAETIENYDLKLEGLRATVEKLIEAHGIHVIAFQEVKSEEVVRHALGKF
                     SGHFEVCVAPHSTFQTVAFAWDKSVLASEYTCIPHRDLAIAEHVGEKHRRVRPGLALE
                     LIVNGLPVTFLNIHLKSGCANLHGSRRWPSRLLTDPEAACTVLNRQVPILEDWIEQVA
                     SRSPRFILLGDFNRRIDQEARARIARTEVRADGSDPAEANIKDEAGGVKSDYLWQEIA
                     DGSPPMYQVYLKSLEPGCKGLAGLDHIVVSGPIKRAQRERLYSSKIAPVKRSRQPIPT
                     SDHCPTITTVEL"
BASE COUNT         1087 a         1250 c         1166 g         1063 t
ORIGIN      
        1 ccttgacaac tcgagcatca ttggctccct ggatgaccat caacggcttc cgtatctctt
       61 ttgcgtgaaa aagtggcgag gtcgcgacaa ggatgtctat atccctcacc ggatcaccta
      121 tttcatcgta aattgccttt ctcaccgact cccaataggg tggaatactt tccagtgtcc
      181 tgagccagtt gctgacccca aatatatcga ctccgacttt aaaaacttcc ggacggaagg
      241 ccagggcgga tagtgtcata tagccgccgt agctcgctcc catgatgccg atccgctccg
      301 gatcgatgta gccaaggctc gccaggtaag tttttgcctc gacgcaatcc cacaacggct
      361 cacgcccatg cttgcgattt gcggccgtaa aaaaagtctt gccgtagccg gagctacccc
      421 ggttgttgat cccgagcaca gcgtacccat ggtttacgag atactgaatc tgcgcgctgt
      481 agccatgcat agtctgcccg ccgggcccgc catggacgta cacgagggca ggaaccttgt
      541 ttgatgagga cgccccctgc ggtttataga atatggaggg tataaccagt ccgtcaaaag
      601 atttgaaacg aacaacctgt ccctccacca gatccatagg atcaatatcc gggctcaggc
      661 tgcaagtcag ctgctggagg cttcccgtgt gtaaatcttg cacgaacagg ttatcaggcg
      721 aacgatctcc gttcacataa aaggccatga aattgccgct ttctgaaaac acaaccccgc
      781 ggatttcacc ctcggggagt cgaggaatag caaggggaat ctctcttttt ctgccatcct
      841 cgtcgacgat ctcgacaacc cgtatcacga tgcttccgtc ctggtttatc gcagtgaccc
      901 ggaatcgccc atttcgggaa aagtacgtgc tgatcacgtc ccaatccgcg ctttccttct
      961 cctcgattcc gcctccggcg agatcataag tacacacccg cttgaattcg cttccctgat
     1021 tggtgagaaa aaacagcttt tccgacgctg gatcgaatgt ttccgcgtgg aaaccgatcg
     1081 acccctcatg ctcagtcaga tgccttgttt gctgcttctc aacgtcgaag aggtatatgt
     1141 ccccgtcaga ggttgtgttg gatcggttca gcgcaatcca gcgttcgtcg cgactaatgg
     1201 gtccgagatc aaacccttgc tcgttcttgt agagcagcgt ccttgtgtag ttccgagtat
     1261 cgtagcgata gagatcaaag aacctcgcat cacgttcatt gcttgaaaca aaaaacgcac
     1321 tgccatcggc tcgccagccg atgaactgcg ccttcaggtt ctcgcccggc gtgaggtcca
     1381 tttcattgcc atcctcgccg agaacgaaca ggtggaagtt ctcattgccg cctcggtcac
     1441 gggtaaaaag gatgcggtta tcggagggga agaaactgac tgcaaaggta ctgtcggtag
     1501 tggaccgcgt gagtggctgt ggctctccga tgccgcatcc cttttcccct ttaggtattg
     1561 ccagggtata gacattgaag acacccgacg cgttgctgct gaaaaggata ctgcgctcat
     1621 cggcactgaa cgaggctccc agaatggaag tcgagttcat gaattgttcg atggtgtaac
     1681 gcttcatatg agcggccagt gatatagggg caccgaaagg ggcaaggacg tcatgggcat
     1741 tatggctcaa accacctgct tcaaccactt catccccaat gtataccacc gaacagaaca
     1801 attaccacca tgaaggcatc atatgcacaa aaaattgcgc ctggaagtgc tggggaatca
     1861 gcgcagtgtg tgaagcttat tctttggccg ggcaagaatg gatcgatcat caaggggatt
     1921 ttaattaccg tgaataattg gaggaatctc aaatgttcaa gaagattgta acccttgccg
     1981 cgcttgcagc cggtggattg atgctgacaa agtatttccg taaaaatcat ggatcggaaa
     2041 agatgtccac tgtcgaagaa tcaatcgagg tgaatgtacc agtcagcact gcctacaacc
     2101 agtggacaca atttacggac ttccccaaat tcatgaaagg agtccaggaa gtgcgacaga
     2161 tcgatccaac acacttgcat tggcgggcga ttgtaggagg taaggaagag gaatgggact
     2221 ccgagatcac ggaacagatt ccggataccc gtattgcatg gcgcagtacg accgggccaa
     2281 aaaattgcgg agtagtcacc tttcacaaga tatccgattc aacctcaaga atcatgctgc
     2341 aaatggatta cgcaccgcag aatgtagcgg aaggaattgg agatatgctg ggaatagtaa
     2401 aggcggagat acacaacaat ctcaagtctt tcaaggaatt cctggaagaa cgcgggaaag
     2461 aaacgggtgc gtggcgagga gcagttcctc agcaataaac agctggcaac aagcaggcta
     2521 tcgggggccc gctatcctgc cagatagcct gcgacccgtg gtcgatgtac ggtaacattt
     2581 agcgagcatc cgggggtcag cagcctgctt gatggcggcc gtaaattcga aaaaaatcct
     2641 gacaattcaa ggacccgggc agggatggaa tgacgggaga cccgacactt ctcatactct
     2701 tgtacttcat cctgcctgtc tggttgcttg ccggtttcgc tgattggctc tgtcatcgag
     2761 cttcccacat cgcaacaacg actggcgcca aagagtcgct actccacctc ctgatgtttg
     2821 cagaaattgg cctgccgctc ctcgcagcga tttttctgca gataaacgct ggaatcatcg
     2881 cactcatgat tgttgcgttt ttcattcacc aggcgaccgc cctttgggat gtgagttatg
     2941 ctgtcaccgc gaggaacgtc tctcctattg agcagcacgt tcacagcttt cttgagatga
     3001 tacccctgat ggcgatcctg tttgtcgtaa cgcgccactg ggatcaattt ctggcgctct
     3061 ttggcttctg cgccgaaccg ccgcgattta gccttgcgtg gaaagaagaa cccctgccag
     3121 ccacctatgt gatctcgatc atggcagcca cgctcctgtt cgaaattatt ccttacgtgg
     3181 aagagcttgc gcggggatta agagccaacc acgggatgct tgtgccaccc aaagcgcgca
     3241 gcaagaacga gtagcacgca ttagcaactc atcgcaataa aacggcccgg caacttgcac
     3301 cgccgggccc ttattccggt catcgatctt atcaggtcca gcttacgccc aagagtccca
     3361 aaccggccac gagtacaaga atgtatgtac tccaaaacat caatacactg ttcatggcgc
     3421 acctccttgc gtgcaggttg atacgcctcc atattacgat tccagcttct cagttctgtt
     3481 cgaaaggccg cacaagacac gtttccttgc aaattgcgcc ctggttataa ctcaacggta
     3541 gtgatggtgg gacaatgatc gctggttggg ataggctgcc ggcttctttt cacgggtgca
     3601 atcttgcttg agtaaagacg ctctcgctgc gcccgcttga tcggcccgct cactacgatg
     3661 tgatccaagc ctgcgagacc tttgcaacca ggttccaggg atttcaggta cacctggtac
     3721 atcggtggag atccatcggc aatttcttgc catagataat cgcttttcac cccgccggct
     3781 tcgtccttta tattggcttc cgccggatcg gagccatccg ctcgaacttc ggttcgggca
     3841 attctcgctc tggcctcctg atctattcgc cggttaaaat ccccaaggag gatgaagcga
     3901 gggctccggc tcgcaacttg ctcgatccag tcctcaagga tgggaacctg gcggtttaag
     3961 acggtacagg ccgcttcagg gtcggtgagc agacggctcg gccagcggcg agaaccatgg
     4021 agattggcgc atcctgactt gagatggata ttcaagaatg taacgggtaa accattgacg
     4081 atcagttcga gggccaagcc gggcctgact cggcggtgct tctcccccac gtgctctgca
     4141 atcgcaaggt cccgatgagg tatgcacgta tactcgctgg cgagtaccga cttgtcccac
     4201 gcgaatgcca ctgtctgaaa ggtgctgtga ggagcgacgc aaacctcgaa gtgcccagaa
     4261 aacttaccta gcgcgtgccg gacaacttct tccgatttca cttcctggaa ggcaatcaca
     4321 tgaatcccgt gcgcttcaat gagcttctcg accgttgccc ttaagccctc cagctttaaa
     4381 tcgtagtttt caatagtttc tgccagagtc ggatgcttcc tgctcccgta agcagtacag
     4441 ggggctacca tcattgccgc atcaagcccg cctgcggctg caataaactg tttctggcat
     4501 agcgtggacc tgacttcctc ctcagcctct ggatggaagg cgccccacga agttctgctc
     4561 cgcgca
//
LOCUS       DASSHJ010000033         8385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_738758, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000033 DASSHJ010000000
VERSION     DASSHJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738758"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..129
                     /locus_tag="VFI43_01625"
     CDS             13..129
                     /locus_tag="VFI43_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HET7060858.1"
                     /translation="MRTMDTMWVSGLMQTRASETEMGQAGYQLKGERIEPYK"
     gene            171..986
                     /gene="ppk2"
                     /locus_tag="VFI43_01630"
     CDS             171..986
                     /gene="ppk2"
                     /locus_tag="VFI43_01630"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HET7060859.1"
                     /translation="MNKENNTKNSTSEELPDREHAKKVYKDTLYKLQVELVKLQRHLI
                     KHDDKILILFEGRDAAGKDGTIKRIIQHLSPREVRVVALGKPSDKDRSSWYFQRYVPH
                     LPTADELVLFNRSWYNRAGVEHVMGFCTEAEYEEFMGTVLPFEHMLIQSGIKLLKYYL
                     DISKSEEAKRLEERKTDPLAQWKLSALDEYALKKWDQYTLARDKMLVHTHDLISPWTI
                     VRADDKKIARLNVIKDLLSRLDYPDKDERLILPDPAIVAQFEAAFIEDGMLAS"
     gene            1455..1883
                     /locus_tag="VFI43_01635"
     CDS             1455..1883
                     /locus_tag="VFI43_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060860.1"
                     /translation="MKKKFAGVLFMLGFLASCAPMSRYEAIDSPVLRQTVENARTRED
                     HEALTRHFETLADEMRSKAEEQRKLLEHYEEKSYLYGRQAQDRQSHTWALMVKYEKAA
                     RASQVYAASHRIIAAKLEQGGYRPGQQAAGVTGKSSSKNN"
     gene            2298..2501
                     /locus_tag="VFI43_01640"
     CDS             2298..2501
                     /locus_tag="VFI43_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060861.1"
                     /translation="MQRWVPLLLLIIFTIGATLALVGMAKNFAQKRTVFADPLCKGAW
                     EKMYRVEHNSPHGKSLEEKSKKS"
     gene            2778..4724
                     /locus_tag="VFI43_01645"
     CDS             2778..4724
                     /locus_tag="VFI43_01645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7060862.1"
                     /translation="MPDINSVFSADGALARNVPGYTVRAQQREMAQAIADTIAANSIL
                     VAEAGTGTGKTFAYLVPALLAGGKVIISTGTKTLQDQLFNRDIPTVRAALKAPVTVAL
                     LKGRANYVCHYHLERTIQDERPSLVSRDEVQYLKLIERYTRTSKTGDKSALSEVPENA
                     AIWQQVTSTRENCLGSECSHYKDCFVMEARKQALIADVVVVNHHLFFADVMLRDEGLT
                     ELLPSCNTLIFDEAHQLPETASLFFGESVSTSQLLELARDTEAEAVRSAKDFAPLPAA
                     VAAMEKATRDFRLTVEGENIRIPMATTMKNGKFSSGLDELMEKLEILAGQLESQAERS
                     EGLENCWQRARELAAGIKRWRDQAEAEGYVRWLEVFSQALQLNATPLSIAEIFNRQLT
                     GSPRAWIFTSATLSVKGDFSHYKSEMGLNSETPVSQSACWESPFDFANQALLYAPSGM
                     PDPSSSHYIQEIVKFALPVLKASGGRAFFLCTSLRAMKRVHELLQEAFARDKLDYPLL
                     LQGQGSRSYMLERFRKMGNAVLIGSQSFWEGVDVRGEALSLVVIDKLPFAPPDDPVLA
                     ARIDKINKEGRNAFMEYQLPRAVINLKQGAGRLIRDEADRGVLMICDPRLITRPYGKK
                     IWQSLPPMKRTRELAEVEVFFGKK"
     gene            complement(5432..7357)
                     /locus_tag="VFI43_01650"
     CDS             complement(5432..7357)
                     /locus_tag="VFI43_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7060863.1"
                     /translation="MQPDQELDVIPLDEAGTLSGLFRIRKERSPESVAYIQFDSESGK
                     WRSYTWQEMGRLVGRWRTALASEGFGAGERVAILLRNSVEWVGFDQAALSLGLVVVPL
                     YPSDSPDNIAYILADSGARLLLVQTQGRWETLAPLCAGLTHLKKVLTVQNLGETGKAL
                     STDEKILLESLDDWLAKVASPFAEEPNPLAKSAGEKYPNEQGEYKYVTHEPDPHALAT
                     LVYTSGTTGRPKGVMLSHHNVLWDAGATLNAIRGYRSDIYLSLLPLSHMFERTVGYYV
                     PMMAGSTVGFARSLKDLPSDIDVIRPTLLVAVPQIYDGIRSKIQKQLDQKGRFARLLV
                     DRAIDFGWERFRAKQHHSGQTPPRRWLNSLIGAVLHPLVADEILSRFGGRLRIGVSGG
                     GPLYAETARFFIGLGLPLIQGYGLTEASPVLAANRLDDNIPASVGLALAGVEIRLGEN
                     DELQARSPGVMLGYWNKPEETRAAIDAEGWLHTGDKASISNSHIFITGRIKEILVTST
                     GEKIPPSDVEMAITQDPLFEQAMAVGENKPYLTALLVLNRGEWEKLAVSLKLEAHEPG
                     SLVNPAARAAVLKRIKSVLKGFPRYARIRAVHLTIEPWRVEDGLLTPTMKLKRGELQK
                     RFAEQIAELYKKRPEAV"
     gene            complement(8138..>8385)
                     /locus_tag="VFI43_01655"
     CDS             complement(8138..>8385)
                     /locus_tag="VFI43_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7060864.1"
                     /translation="DKLISVVALYMGIQALETYVLTPLLTQKMVDLPPALVITMEILM
                     GVLAGGLGLVLATPLTAAGMVMVEMVYVEDVLGDHSS"
BASE COUNT         2019 a         2205 c         2222 g         1939 t
ORIGIN      
        1 cctgtggcaa acatgcgcac catggatacg atgtgggtga gcgggcttat gcaaaccagg
       61 gcaagcgaga ctgaaatggg acaggcgggc tatcaactca agggcgaaag aatcgaaccc
      121 tacaagtagc agtactcccg cgcgcgtgca gcattggaag ggaaacggcg atgaacaaag
      181 agaacaacac aaagaacagc acgagtgaag agctgcccga tcgcgagcat gcaaaaaaag
      241 tttacaagga tacgctctat aagcttcagg tcgagctggt caagctgcag cgccacctca
      301 tcaaacacga cgacaagatc ctcatccttt tcgaaggaag agacgcagcg ggaaaggatg
      361 gtacgatcaa gcggatcatc cagcacctga gtcccaggga agtgcgggta gtcgcactgg
      421 gtaaaccgtc cgacaaagac agaagctcat ggtatttcca gcgttacgtt cctcatctgc
      481 ccactgccga cgaactcgtg ctgtttaacc gaagctggta caacagggct ggagtggagc
      541 acgtaatggg tttttgcact gaggccgagt atgaagaatt catgggcact gtgcttccat
      601 tcgagcacat gctgatccaa tcaggtatca aactgctcaa gtactatctc gatatcagca
      661 agtccgagga ggcaaagcgg ttggaagaac gcaaaacaga cccgctcgcg caatggaaac
      721 taagcgcgct ggatgaatat gcgttgaaaa aatgggacca gtacacgctc gctcgggaca
      781 aaatgctcgt tcatacccat gatctgatct cgccctggac tatcgtgcgc gcggacgaca
      841 aaaagattgc gcgcttgaat gtcatcaagg atttgctcag ccgtctggac taccctgaca
      901 aggatgaacg cttgatcctg ccggacccgg cgatcgtcgc acaatttgaa gcagccttca
      961 tcgaggacgg tatgctggct tcatagctgt gctggtgaag cgtttcaagg caaaataatt
     1021 taccttctta ccaccccgtc ttgacctgat ggggtgcggc ccttcgggct ccacctcctg
     1081 cccattttaa tactcccctt ttagcatctc ccgtagcaca gatgcgcttg atcttttatc
     1141 tgaaggccaa aaagaacgtc tgaacctgca acgccgatag cgggtctgcg catgcaatgg
     1201 gatgtgccag aaaagggtcg gcgcgataag caagatgcgg atgtaatccc cggaatctta
     1261 tagcagtcac ggctcggatg tccgtattcg cctcttcata ttccccatta cctgccttca
     1321 aacttgatcg cccctcactt aacttacttt taacatatga aagttgacaa agtagctaca
     1381 gaaaggtaag cttgtttcaa ctgcccgggg attctgtcag gcagccaggt tttagactca
     1441 caggggtgcc gaaaatgaaa aaaaaatttg caggtgtttt attcatgctc ggttttttag
     1501 cttcatgcgc tcccatgagc cggtacgaag caatcgacag cccagtcctt cggcaaactg
     1561 tcgaaaatgc aaggacgcgt gaggatcacg aggcgttgac gaggcatttc gagacattag
     1621 ccgatgagat gcgaagcaag gccgaggaac aaagaaagct ccttgagcac tacgaagaga
     1681 agagctacct ctacgggcgg caggcccagg acaggcagtc tcatacatgg gcattgatgg
     1741 tcaagtatga gaaagcagcg cgggcgagtc aggtatatgc tgcctcacac cgtataattg
     1801 ccgcaaaact cgagcagggc ggctatcgtc ccggacagca agccgcgggt gtgactggta
     1861 agtcaagctc taaaaataac tgaaagagga gtacgtcgct ctcgaattct ggaaagcctt
     1921 gcatgtcgcg atacctgttg caagtcgttg cttgtttgtt ttttggtcgc ttctttattt
     1981 aaaggaaacc aaggcaaaat gaaaagctat agcggcagtg acccggccaa aatgttttgc
     2041 tgtcccttcg atcagggatc ggaagtgttt cggcagaaga tgttcaggca ttttgaattc
     2101 catgattgtc gagggacagt agaagctgga acaacgccgg aatgggaaac accggccgcg
     2161 ggttcgggat ttgtctcctg attccaacaa cccagccgag tcctctgcaa attccaattt
     2221 ctagttcaag tcaggtgctt gacgcaagca cctgaagcat tacaattgaa ttgatccttc
     2281 cctgaacgga ggaaaaaatg cagagatggg ttccattatt gctactgatt atctttacga
     2341 ttggcgcaac gcttgcgctg gtcggtatgg cgaagaattt cgctcagaaa cggaccgtgt
     2401 ttgcagatcc tctttgcaag ggcgcctggg aaaaaatgta ccgggttgag cataattcgc
     2461 ctcatggaaa atcattagag gaaaagagca aaaaaagctg acgtttgttc ttatcgagat
     2521 gctgacgctc cgcatctcgt ttcccccgca ctttatttct ggggcaggcg agagcgtatc
     2581 aggagttatc tccaacctcc ctgccgtgca cctggatcag gaaaggaggt ctgcttcgcc
     2641 tcgctcagcc ttgacatcct gtgcaaagcc tgccgaagca acttttcccc gggcctcgtt
     2701 tactttttca cacccgtgcg cttctcctag aactgctgca tgaggtatga tggcgggctg
     2761 cttcgccgaa ttacttgatg cccgacatca actctgtttt ctctgccgat ggtgcgcttg
     2821 cgcgcaacgt tcccgggtat accgtgcgcg cgcagcagcg cgaaatggcc caggcaatcg
     2881 ctgacaccat cgcggcaaat tccattctcg tcgctgaggc cggcaccggc acaggcaaga
     2941 cctttgccta cctggtgcct gccttgcttg caggcggcaa ggtcatcatc tccaccggaa
     3001 ctaaaactct tcaggatcag cttttcaatc gcgacattcc taccgtgcgc gcggcgctga
     3061 aggcgcctgt caccgtagcg ctgctcaagg gccgcgccaa ttatgtttgt cattatcacc
     3121 tggaacgcac tatccaggat gagcgcccga gccttgtcag ccgcgacgag gtccaatacc
     3181 tgaagttgat cgagcgctat acgcgcacca gtaaaaccgg tgacaaaagc gcgctgagcg
     3241 aagtcccgga aaatgctgca atctggcagc aggttacctc aacccgggag aattgtctgg
     3301 gctcggaatg ctcccattac aaggattgtt ttgtcatgga ggcgcgtaag caggcgctca
     3361 ttgcggatgt cgtggttgtc aatcatcacc tgtttttcgc ggacgtcatg ctgcgcgacg
     3421 aagggctgac ggaactgctt ccgtcctgca ataccctcat attcgatgag gcgcaccagc
     3481 ttcccgaaac cgcgagcctg tttttcggcg aatcagtgag tacaagccag ttgctggaac
     3541 ttgcacggga tactgaagct gaagcagtga gaagcgcgaa ggactttgct cccctccccg
     3601 ccgccgtggc tgcgatggaa aaggcaacgc gagatttccg cctgactgtc gaaggtgaga
     3661 atatccgcat accgatggct accacgatga aaaacggaaa attcagcagc gggctggatg
     3721 agctaatgga aaaactggaa atactggctg gacagctgga aagccaggct gagcgctcgg
     3781 aagggctgga gaattgctgg cagcgggctc gcgaacttgc tgccggaatc aagcgctggc
     3841 gggatcaggc cgaagctgag gggtatgtac gatggttgga agtattcagc caagcgttac
     3901 agctcaatgc gacgccgctc tcgattgcag agatattcaa caggcagctg acgggctcgc
     3961 ctcgcgcatg gatatttact tcggccacgc tttccgtaaa aggtgacttc tcacattata
     4021 aaagcgagat ggggctgaat tcggagacgc ccgtttccca atcggcctgc tgggaaagcc
     4081 cgttcgactt tgccaaccaa gccttgcttt atgccccttc cggcatgccg gatccgagca
     4141 gctcgcatta tatccaggag attgtcaaat tcgcgctgcc ggtattgaag gccagcggcg
     4201 gtagagcttt ttttctatgc accagtttga gagcgatgaa acgcgtgcat gagttgcttc
     4261 aggaagcgtt tgccagggat aagctcgatt acccgttact tttgcaggga caaggctcac
     4321 ggtcatatat gctggaacgg tttcgtaaaa tggggaatgc cgtgcttatc ggaagtcagt
     4381 ctttctggga gggcgtggat gtccggggcg aagctttatc actggtggtg atcgacaagc
     4441 tacctttcgc gcccccggat gaccccgtcc ttgccgcacg catcgataag atcaacaagg
     4501 aggggcgcaa cgccttcatg gagtatcaac tccctcgagc ggtcatcaat ttgaagcagg
     4561 gagctggacg gctgatccgt gacgaagcgg accggggtgt gttaatgatt tgtgaccctc
     4621 gcctcatcac caggccttat ggaaagaaga tctggcagag cctgccacct atgaagcgca
     4681 cgcgggaact cgctgaagtg gaagtgtttt ttggcaaaaa atagacttcc gcagctccag
     4741 ccctttcatt ggggttaggg tatcaagagc gatcgcgtag acctgtaacg ccttcctgct
     4801 cagcgcaatg ggcgcagcag aacatcaccc cctccttttc cagtccgtgg cctattattc
     4861 gtacaccgca gtgcgtgcaa ctgggggcaa gggtttggat tgcgcattcg aagcaatcaa
     4921 acgtgtaggt tttatccgaa acaatgatct gaaaggattt gtcgtagttg tttccgcatg
     4981 cgtcgcattt tggcataagg ccccctctat cgagcgttga aaagtcacta taggcatagc
     5041 atactttcag ggacaagcag tttcatcatg attcgatacc gctctcaccc cattcggttg
     5101 catcccgatc acggaagagt gaagttctcc ccgggctcca ttgttttcga ttgatgcttt
     5161 gcttaccgca ccattgcgct aatccgttcc cggctaatgc ccctctcgaa accgcagatc
     5221 tgacttctac ttccgaatca ttaaatggtt taaccgaaat ttaacagtct gctaaccttc
     5281 gggcgagatc gcctgatgga cctggccaat ggtggaactt acccttaaaa ggagacggga
     5341 caggttatag aaccgcggct acgagaagaa gtttaccaat aacccgctat tagaccggtt
     5401 attcaagagg ccgtattgga cagcaacgcg atcacacggc ctcaggccgt ttcttataga
     5461 gctctgcaat ctgttctgca aaccgcttct gcaattcgcc gcgcttcaat ttcattgtag
     5521 gggtaaggag cccgtcctca acccgccacg gctcgattgt aaggtggaca gcgcgaatcc
     5581 gcgcatatct tggaaatccc ttcaagacag atttgattct cttcaagacc gccgctcgcg
     5641 ccgccggatt tacaagtgat ccgggttcgt gagcctccag tttgaggctc actgcaagtt
     5701 tctcccactc ccctctgttg agtaccagga gcgcggtgag gtaaggttta ttctctccca
     5761 ctgccattgc ctgctcgaat agcggatctt gcgtgattgc catctcgacg tcgctgggag
     5821 gaattttttc tccggtcgaa gtgacgagaa tttccttgat gcgaccggtg atgaatatgt
     5881 gactattcga aatgctggcc ttgtcacccg tatgcaacca gccttcagca tcaatcgcag
     5941 cccgggtttc ttccggcttg ttccaatacc cgagcatgac gcctgggctg cgcgcctgga
     6001 gttcatcatt ctcacctagc cgtatctcaa ctccagccaa ggccaggccg accgatgctg
     6061 gaatattatc atccaggcgg tttgccgcca gtacgggtga agcctctgtc aatccgtagc
     6121 cctgaatcag cggtagtccg agaccaatga agaatcttgc agtttcagcg taaagcggtc
     6181 cgccaccgct cacccctatt cgcaaccttc cgccgaaccg tgacaggatt tcatccgcga
     6241 ccagtgggtg aaggactgcg ccgatgagag aattaagcca acggcgaggt ggcgtctgcc
     6301 ctgagtgatg ctgtttcgcg cgaaagcgct cccagccaaa atctatcgcc cggtcaacca
     6361 gcaggcgggc gaacctcccc ttctgatcca actgtttttg tatttttgat cgaataccat
     6421 cgtaaatttg gggcacggca acaagcaagg ttggacgaat aacgtcaatg tcgctcggca
     6481 aatccttgag cgagcgtgca aagcccaccg tgcttcccgc catcatgggt acgtaatagc
     6541 cgacagtgcg ctcaaacatg tgagaaagcg gcagcagcga gagatagatg tcagaacgat
     6601 agccgcgtat cgcgttgagt gttgcccccg catcccacaa aacgttgtga tgggaaagca
     6661 tgacgccttt tggccgcccg gtagtgcctg aggtgtaaac aagtgttgcc agggcgtggg
     6721 gatcgggctc atgggtaacg tacttgtatt ccccctgttc gttcggatat ttttcgcccg
     6781 cgcttttagc aagcgggttg ggctcttcag caaagggtga agccactttc gcgagccagt
     6841 catccaggct ctccagaagg atcttttcat ccgtgctcaa cgctttcccg gtctcgccca
     6901 aattctgtac cgtcaaaacc ttcttcagat gggtcagccc agcgcataat ggagccagcg
     6961 tttcccaccg gccctgcgtt tgcacgagga gcaggcgggc acccgaatca gcgagaatgt
     7021 acgcgatatt gtccggtgaa tcggatggat aaagcggaac gacgaccagt ccgagagaca
     7081 gcgcggcctg gtcgaaaccc acccactcca cggagtttcg caagagtatg gccactcgct
     7141 ccccggcacc gaagccctcg cttgccaaag ccgtcctcca gcggccgacc agacggccca
     7201 tctcctgcca ggtgtagcta cgccatttgc cgctctcaga atcaaactgt atgtaagcga
     7261 cggattcggg gcttcgttct ttgcgtattc tgaacaggcc ggataatgtc cccgcctcat
     7321 caagtgggat cacatccagt tcttggtcag gctgcatctt taaatgagac gagcacgttg
     7381 aaaactcgcg atcaacagga gacagagata ttgaagcaac atttcctcat attcttttca
     7441 ccgacaaagc tgcttccacg aattcgcata ccggtttttc gctttgccgg ctttctggtt
     7501 ttgcgtcagt tttcatagta gattaaatcc ctttaaaatt ctgtgcctaa gcggcatgca
     7561 tcaagtcata cggcggcgcg tcaactgcaa gcgaggcatg tattcgcgag aatggtcacc
     7621 ggtggatact ggacaacccg acgatggaat gcagggggta tgtgcgcgtt cgtctagtgt
     7681 cttgccggga gcgaatttga cagggaggtc ggatttgagg gaagtgaagg atcaatgtac
     7741 cgaataagct ttttacttgc ctccgttata atgcctgcga agcaagtgcg gagtcggcta
     7801 tatcgatcta tccatcgctg ataatacgta acatgcagat tcaggagcgc tcccttccac
     7861 tcctgaggaa gggagcggct tagataaaaa agagcgctca gtggctctag gagaaaataa
     7921 agtcggcggt gccgttcagt ttcagcgtct catatgtatc cacttgcctt cgggtcttgt
     7981 taaaggggcg gttacgactt aaatctgcct taccgctata gctgagtatc ggcttgccag
     8041 ttatgctgtc tgctgaaaag tcaatttgct tattctctct ttcgagaatg aaatggttca
     8101 ccaaggttaa aatcggctgg ttgtaaaggt cgtttactta cgacgagtgg tctccgagaa
     8161 catcctccac atacaccatc tcgaccatta ccatgcctgc agctgttagc ggagtggcca
     8221 gaacaaggcc gaggcctccc gccagcactc ccattaatat ctccatggtg atgacaaggg
     8281 caggtggtag atcaaccatc ttctgcgtta acaacggcgt cagtacatag gtttcgagcg
     8341 cctggattcc catatacagc gcaaccacgg aaataagctt gtcag
//
LOCUS       DASSHJ010000034         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1282023,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000034 DASSHJ010000000
VERSION     DASSHJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282023"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFI43_01660"
     CDS             <1..274
                     /locus_tag="VFI43_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HET7060865.1"
                     /translation="LAEYPDFGGELLNHFGGDVEEAKTSAKDNYCGCYRSLADYAQEL
                     TEETTQIPEHLAYYIDYERMGRDMEMSGDIFTIETGFETVHVFWSR"
     gene            complement(225..989)
                     /locus_tag="VFI43_01665"
     CDS             complement(225..989)
                     /locus_tag="VFI43_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060866.1"
                     /translation="MHRKQVYILARGDRRGFVAVDHRGEVFAVSKWAGIKTKEVRPRL
                     TDEKNLPSVDEARSQIAKDMTARLETFSQEQITVFATHRAELEEKRQALVRQYQAERQ
                     KLIEAQQSYWRDKQQEWRNNFNKGIRGLFDRLTGRRKQIEERNEQDAWQVGMRQQQER
                     DALIFEQLETRRTLQARIERLEALKSYRFNELEHDRSQYQAMRDQRLEQLETQRQEKN
                     RDRPAASPARPGMDALAMGSINGSRIHAPFQSRFRL"
     gene            988..1287
                     /locus_tag="VFI43_01670"
     CDS             988..1287
                     /locus_tag="VFI43_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060867.1"
                     /translation="MGESILCFTERPAFLKYGFNLLRVFANTLGLLPFRQREIKWITR
                     MRMLKSFRHCPAVFQKQFTGQVEQSSFGERKLYRFHFISLILDQTAWQCLRPFFS"
     gene            complement(1248..1583)
                     /locus_tag="VFI43_01675"
     CDS             complement(1248..1583)
                     /locus_tag="VFI43_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060868.1"
                     /translation="MILVGNQRGGAKDLALHLLKQENEHVEVHEVRGFASDNLMAALN
                     EAYAISRATHCKQFLFSLSLNPPKHENVSTETFKQAIERAEKKLGLNDQPRAIVFHEK
                     NGRRHCHAV"
     gene            complement(1580..2023)
                     /locus_tag="VFI43_01680"
     CDS             complement(1580..2023)
                     /locus_tag="VFI43_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060869.1"
                     /translation="MSGFTNIPSAVQAFSAATAPLPKKSKRRYTPPFSLRLTVDERKR
                     LDELAGNQPLGSYIRDRLLGEQTEKRRKTKKPTPDAALLALVLGEFGRSRLASNINQL
                     AKAANIGTLDVTPETEREIVQACAEIQATRVLLITALGVAPVEDE"
     gene            2132..2944
                     /locus_tag="VFI43_01685"
     CDS             2132..2944
                     /locus_tag="VFI43_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7060870.1"
                     /translation="MTKAVLTTKVSPVYDDLPEIRYHFPKTYLRQLIAAVDDWIVYYE
                     PRRSSGDLSSRGGRQVYFATARVDRIIPDPVHENHYYAEVSNYLPFTVPVPFKEGSHY
                     YEKNLKKADGSTNKGAFGRAVRNLEDVEFDRIWKAGFGQILGFEERVGVDPDMPEKPT
                     ERIEVFFARQPESSSYVPMEQDRPIVQQLISRPFRDKAFSAAVKTAYGDTCAVTGLKI
                     INGGGRSEVQAAHIRPVQHQGPDSIRNGIALSGTVHWMFDRGLYLSMTITSF"
     gene            3259..4161
                     /locus_tag="VFI43_01690"
     CDS             3259..4161
                     /locus_tag="VFI43_01690"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HET7060871.1"
                     /translation="MTAPSLKLAIVVLVLVLSTLAGCSVVRFGYNQADHLTYWWIDRF
                     VDFNDAQKPRVREALAQWFSWHRRSHLPGYAALLVNSQNLVLEDITPERTCGLWTDLR
                     GYMDSAFERALPMTAEFVLTLTPQQIQNIERRYAKINEEFSDEHLQSNEARRLKKSIK
                     RTIERAEFFYGDLDKAQEAFIAEWVTRSPYDVNIWNKERHRRQQDLVQVLQRLNVENS
                     TQDKAKAVLRAYLERIYSSSDENYRKYAEQVWIYNCAFVANLHNTMTVAQRKNAVKKL
                     KDWESEIRALLPRTDAAQLSPSFP"
BASE COUNT         1023 a         1104 c         1128 g         1131 t
ORIGIN      
        1 ccttgccgaa tatccggatt tcggtggtga actgctgaac cattttggtg gtgacgtgga
       61 ggaagcaaaa acaagcgcca aagacaatta ttgcggctgc taccgatcac tggccgatta
      121 cgcgcaggaa ttaaccgagg aaacgacgca gattcctgaa catctggctt actacatcga
      181 ttatgaacgc atgggccggg atatggagat gagcggcgat attttcacaa tcgaaaccgg
      241 ctttgaaacg gtgcatgtat tctggagccg ttaatgctcc ccatcgctag cgcgtccatt
      301 ccgggccgcg ctggcgaggc tgcgggacgg tcccggtttt tctcttgacg ctgtgtctca
      361 agctgttcga ggcgctgatc gcgcatggct tgatattgcg acctgtcgtg ttccagttca
      421 ttgaaccggt aggatttcag cgcttccagc cgctcgatcc gtgcttgaag ggtacggcgc
      481 gtttcaagct gctcaaaaat cagcgcgtcg cgttcctgtt gctggcgcat tccgacttgc
      541 catgcatctt gttcattgcg ttcttctatt tgcttgcgcc ttcctgtgag acggtcaaac
      601 aggccgcgta tgcctttgtt gaaattattt cgccattcct gctgtttgtc ccgccagtag
      661 gattgctggg cttcgattag tttttgccgt tctgcctgat actgacgtac caatgcttgc
      721 cgtttttctt ccagctcagc ccggtgagtt gcaaataccg ttatttgttc ttgggagaat
      781 gtttccagcc gggccgtcat gtctttggca atctggcttc tcgcttcatc gaccgatggc
      841 aggttttttt catcggtaag acggggacgc acttccttgg ttttgattcc agcccacttg
      901 gatacggcga acacttcgcc ccggtgatcc accgcgacaa agccgcgccg gtcgccacgg
      961 gcgaggatat atacttgttt tcggtgcatg ggtgaaagca ttctgtgttt tactgaaaga
     1021 ccagcattcc tgaaatacgg ctttaatttg cttcgggtct ttgccaatac gcttggcttg
     1081 ctgccattcc gccagcgtga aattaagtgg atcacgcgca tgagaatgct taaatccttc
     1141 cgccattgtc cagccgtgtt ccagaaacaa ttcacgggac aagtcgaaca atcttcgttt
     1201 ggagaaagaa agctgtaccg ctttcatttc atcagcttga ttcttgatca aaccgcatgg
     1261 caatgcctgc gcccgttttt ctcatgaaaa acgatggcgc ggggctgatc gttcagtcca
     1321 agttttttct cggcccgctc gatggcctgt ttgaatgttt cggttgaaac attctcatgc
     1381 tttgggggat tgagacttaa ggagaataaa aactgtttgc agtgcgtggc gcggctgatc
     1441 gcataggctt cattaagcgc agccatgaga ttgtctgagg caaacccgcg cacctcatga
     1501 acttcgacat gctcattctc ttgtttgagc aaatgtaagg ccaagtcctt ggctccgcca
     1561 cgctggttgc cgacaaggat cattcatctt ccacgggcgc tacacccagc gctgtgatca
     1621 atagcacgcg tgtggcttgg atttccgcgc atgcctgtac aatctcacgt tccgtttcgg
     1681 gtgttacatc cagcgttcct atattggccg ctttggcgag ttggttgata ttggaggcca
     1741 gccgcgaccg accgaattcg cctaatacca gagccagcaa ggcggcgtca ggcgtaggtt
     1801 ttttggtttt acggcgcttc tcggtctgtt cgccgagcaa gcgatcacga atatatgacc
     1861 ccagcggctg atttcccgcc agttcatcca gccgtttacg ttcatcgacg gtgagccgca
     1921 gggaaaacgg aggcgtataa cgccgtttag atttcttggg caggggcgca gtagcagccg
     1981 agaatgcctg aactgctgag ggaatattgg tgaagccgct catggtgacg gctcctgata
     2041 atacgagaca ttactcttga gatagtcgtt ccattcctct agaatttcca gaacgcggtt
     2101 cgaaggttca cactccgtct ccgccattta gatgacaaaa gcggttctca caacgaaggt
     2161 ttcgcctgtt tatgatgacc ttccggagat tcgctatcat ttcccaaaaa cctacctaag
     2221 acaactgatc gcggctgtcg acgactggat cgtctactat gagccgaggc gttcgagcgg
     2281 tgatctgtcg agtagaggtg ggaggcaggt ttactttgcc accgcgcgcg tggaccgtat
     2341 tattcctgat ccagtccacg aaaatcacta ttatgcggaa gtctctaatt acctgccatt
     2401 taccgttccg gtgccgttta aggaaggctc gcactactac gaaaaaaact taaagaaggc
     2461 cgatggctcg accaataagg gcgcgtttgg tcgtgctgtt aggaacctgg aggatgtaga
     2521 gttcgatcgt atctggaaag ctggcttcgg acagattctt ggcttcgagg agcgggtagg
     2581 ggttgatcca gatatgccgg aaaaaccaac ggaaaggatt gaagtttttt tcgcacgaca
     2641 acctgagtct tcctcttatg ttccaatgga gcaggaccga ccgattgtcc agcagctcat
     2701 atcaagacct ttccgggaca aggcattctc agcagcagta aaaaccgctt acggggacac
     2761 atgtgctgtt accggcctta agatcatcaa tggaggagga cgctcggaag tgcaagcggc
     2821 acacattcgg cctgtccaac atcaagggcc cgatagtatt cgtaatggta ttgcactttc
     2881 tggaactgtc cactggatgt ttgaccgggg tttatatcta tcgatgacga ttacaagctt
     2941 ctaatagcaa gggatcgcct ccccgatacg atcgaacgat tgctaaacga taattccaag
     3001 ctccgcctcc ctgcacggcc agatttatat ccgcatccga aatttcttga ctatcatcgg
     3061 cgggaaattt tcaaaggcta aaagaccgtt cactaccatc gcgacgatcg ctgccccgag
     3121 cgaagctcgc tgcgatttca gcccgcattg atagcatact ccagctagag catgcaggat
     3181 cacagtgatt tgaccgaggc gcgactgaag gtagaatatc gtagatgtga attctgatgt
     3241 aatgcccgtg ttatcgttat gaccgcacct tctttaaaat tagcgattgt cgttctggtt
     3301 ttagtgctgt cgacacttgc cggatgcagc gtagtccgtt tcggctataa ccaggcagac
     3361 cacctgactt attggtggat tgaccggttt gtggatttta acgacgcgca gaaaccgaga
     3421 gtccgagagg cgctcgcgca atggttttca tggcatcggc gttcgcacct acccggttac
     3481 gccgccttac tcgtaaactc gcagaatctc gtgctggaag atattacacc cgagcgcacc
     3541 tgcgggttat ggaccgacct gcgcggttat atggacagcg cgttcgaacg tgcgctgccc
     3601 atgacggccg agttcgtgtt aacgctgacg ccgcaacaga tacagaatat cgagcggcga
     3661 tatgccaaaa tcaatgaaga gtttagtgat gaacaccttc agagcaacga agcccggcgt
     3721 ctaaaaaaat ccatcaaacg gacgattgaa cgcgccgagt tcttctacgg cgatctcgac
     3781 aaagcgcaag aggcgtttat tgcggaatgg gttacgcgct caccctacga tgtcaatatc
     3841 tggaataaag agcgccatcg gcgccagcag gacctcgtgc aggtgttgca gcggttgaat
     3901 gtggaaaatt caacccagga taaggcgaaa gcggtgctac gcgcttactt ggaacgtatc
     3961 tacagctcat ccgatgaaaa ttatagaaaa tatgccgaac aggtatggat ttataactgc
     4021 gcctttgtag caaatctcca taatacgatg accgtggcac aacggaaaaa tgctgtaaag
     4081 aaactcaagg attgggaatc cgaaatccgg gcactcttgc cacgcacgga cgcagcccag
     4141 ctcagtccat cgtttcctta aatagtattt ctatggaata agccgacttt tagaccagtt
     4201 aatatggccg aagaagtacg tactgaagtc agattcattg cgccatcata gtggcggtcg
     4261 tccgctcaat cgagttgagg cagttacctg tcgtgacttt gaaaaagata ttcgatctgc
     4321 tataaagctt taagaccccc gctttatcct cccgcccatt tcaaggtcga gctattgcaa
     4381 ttcttt
//
LOCUS       DASSHJ010000035         9200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1062823,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000035 DASSHJ010000000
VERSION     DASSHJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062823"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFI43_01695"
     CDS             <1..393
                     /locus_tag="VFI43_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET7060872.1"
                     /translation="PANRFVADFIGQGVFLPGKMLDPRNVEIELGVLSGTVAHECTVD
                     HCTLCRPGSPVDVLIRPDDIVHDDASPLQAVVLKKMFRGADILYTLRLPGGATVLSLV
                     PSHHNHPIDGKIGIKLEVDHVVAFCPSK"
     gene            739..1467
                     /locus_tag="VFI43_01700"
     CDS             739..1467
                     /locus_tag="VFI43_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7060873.1"
                     /translation="MAKMNKILIVVTNADEFEKAGYRTGLWLSELTHFWDVVQEAGYQ
                     MDIASISGGKIPIDPESLIIAEVGQAVGMEGSVSKRYKDKAFMKLLDDTLRVSDVDVS
                     TYDAIYMTGGHRVMFDFPKSSALADLVARFYESSKIVSAVCHGPSGLLEAKLSNGSYL
                     IENKDVTGFSWNEEVLAKRDNAVPFSLEEELQKRGAKYSKALLPFNAHVIEDGILITG
                     QNPGSARAVGKAVVGKLTEMARSR"
     gene            complement(1519..2511)
                     /gene="mltB"
                     /locus_tag="VFI43_01705"
     CDS             complement(1519..2511)
                     /gene="mltB"
                     /locus_tag="VFI43_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HET7060874.1"
                     /translation="MRLLTCAIGFFALAGCVTSARIPASELRPEIREFIGEVAERHGF
                     PEADLENILRQAQFQPGIINTISRPATSKPWHEYRLLFITPKRIAGGVAFWNAHAETL
                     GRARREYGIPEEIIAAIMGVETFYGAQTGKHRVLDALTTLAFDYPRRAVFFRDELEQY
                     LLLQREKNADLFGTKGSYAGAIGIPQFMPSSYRKYAVDFDNDGKIDLAGSTADAIGSV
                     ANYLKSYGWEAGLPVAVPALVNSEKYREALFTGNWPSYTVADVRTLGIAPQVNVPGNP
                     YATVIELENQGGNEYWLGFNNFYVITRYNRSVNYAMSVLQLAQEIRVARNSGLP"
     gene            complement(2636..6235)
                     /locus_tag="VFI43_01710"
     CDS             complement(2636..6235)
                     /locus_tag="VFI43_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7060875.1"
                     /translation="MSNPLSGTQTAPSPYIPDTAHPGNMPQAPQASSVPDLAERRRAL
                     DPTQSFIVQAPAGSGKTGLLIQRYLKLLAYVNEPEEIVAITFTRKAAGEMRERVTKAL
                     ARARESAQPAGAQTEHERTMRELASAAAARDTRAGWHLAENPVRLRIMTFDSLCASLN
                     RQMPVLSGFGHQPEIVEDASGLYLSAASATIDLIETDGSAASEVRRLLEHLDNDVPRV
                     QSLLAGMLARRDHWLRHIHGRDRDELEAALRRICRDGLKRLREVIELHPRFIHEELVE
                     LLRYSAGNLAASGKNGGGLDYEQLSQFDALPGQEEKDFDAWCALAGLLLSKEGMWRKQ
                     HTVNDGFPTVTKGRKDTAKEWKERACNLIQSLAAGDARDLLRLALHDVRQLPPHSYSD
                     NQWDVLGAIIRLLPHAVAQLRLVFQSHNKVDFSEMAQGALRALGDADLPTDLALALDY
                     QVRHLLIDEFQDTSISQYELIQRLTAGWEPGDGRSILAVGDPMQSIYRFREAEVGLFL
                     RVRTAGMGNIPLQQVSLSANFRSQRGIVEWVNNTFARILPEHEDVTLGATPYTPSVAV
                     RETLDGIAVAIHPFFNDDHFAEALRVTEIVKRVRSDDPSGSVAVLVRNRSHLHEIAPK
                     LRDAGLRFRAIDIEGLAFRPVVQDLLALTRALIHLGDRIAWLAVLRAPWCGLTLADIH
                     ALMAGRPETSVTTSAIAADNDSEGKNAVANDRTVWEWLNDDSRVAAVSVDGRARLSRL
                     RDVLKVCIGNRCRQSLRNTVEAAWLALGGPACLDSEGDLGDAEAYLDYLEARENSGEM
                     EMDGLEEGLTRLYALPDLSADGLLQVMTIHKAKGLEFDTVILPGLGRSPRSDARKLFM
                     WMENLHGDSGKDEVSRGDELLLAPIQETGGEPDRIYDWLEKLNCEKARFEDERLLYVA
                     ATRARKRLHLLGSTGLTVNPDGRYELKEPARKSLLKKIWTVAEPVYEDSCRSVSLSLH
                     AGSQELGDSAIDQSLRRLAPDWALPESPGAVRWKAGMRANPMHVDIEYSWAGETARHI
                     GNVVHRWLKIIAETELEGWTVARIQALHGVFRKQLMAEGMDQNNDDDLAAGRVAKALI
                     HAVSDARGRWLLGPQNDAANELRMTAIIEGRPQDVVIDRTFCDASGQRWVVDYKTSSH
                     EGAGLEGFLSREQDRYRSQLDRYAGLLRKNGQSVRRGLYFPLLRGWREWGDEI"
     gene            complement(6232..8988)
                     /locus_tag="VFI43_01715"
     CDS             complement(6232..8988)
                     /locus_tag="VFI43_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7060876.1"
                     /translation="MSSKTDFPSIPLSDVFDDLASGSRGSLTVLTPNRRLARAVKSEF
                     DRIQAKSATAWNSADILPISAFVERIYSEALYSSEAAALPVLLSPIQERVLWEDAINC
                     SDSGRELLDVGEAARLASEAWQLAHAWHLIPRLDRHLLNEDARAFRQWSRQYEETARR
                     RTDKARVGGLVLELLARPEIAKPRRLVCYGFDIVTRQHAALLTRLKEVGCEVVIAQAP
                     RLPVTTRRKLQRLECDDVRDEMQRAAQWARANLETVPSARIGIVAPDLAARRSAIFRI
                     FSAVMEPDVRQGLAQPDRELPFNISLGEPLVSYPLVNAALLLLDLSTGKVAFEQVSVL
                     LRSPFISGGEVEMIQRAWLDIRLRKRAEEMVTLDRLLVLLERERNAIRCPDLLRILAA
                     LHGFSKTALGGLQKPSALARGIAEALRLIGFPGERRLDSPEYQVLKKWQEVLADFATL
                     DSVISRTTCKHAVTRLRRMAAEVIFQPETADVPIQILGALESAGMTFDHLWVMGLSNE
                     AWPPQARPNPFLPVELQREVPWSSAQATLELARTLTDHWLGAADDLVLSHPRHGQGSE
                     TQELSFSPLIESISAGALAPPLYPAYRDLIHEARKLERLDESDVPALRIAPDARVSAE
                     VHGGTAVIKDHAACQFRSFALYRLRGERPERPPFALGPAERGTLIHHVLAEVWRKLKT
                     KDALDAINEPDLEVLLRGAANDAIDRLRRDHPAAMSRHFSEIERRRLIRLAREWLSQD
                     RKRADFSVVAVEEKRTIEIGGLALTARLDRVDELADGRRVIIDYKSRAPTAAAMLDER
                     PEEPQLPLYLVAGESEAVAVAFAQVKPGDSRFVALARDDDLLPGAVAFPGPRLKVKHT
                     SWNELVDGWRTNLVSIATSFSAGSARMNPRKYPHTCRNCEFRSLCRIDERVGGAFGQD
                     EESE"
     gene            complement(9112..9192)
                     /locus_tag="VFI43_01720"
     CDS             complement(9112..9192)
                     /locus_tag="VFI43_01720"
                     /inference="COORDINATES: protein motif:HMM:NF023379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3470 domain-containing protein"
                     /protein_id="HET7060877.1"
                     /translation="MIEKKDALPEADEWAKVTDKMEKLER"
BASE COUNT         1966 a         2695 c         2485 g         2054 t
ORIGIN      
        1 cccgctaacc gctttgtcgc ggactttata gggcaaggcg tctttctccc gggaaaaatg
       61 cttgatccac gtaatgtgga gatagagttg ggtgttctgt caggaacggt cgcccatgaa
      121 tgcacagtcg accattgcac gttatgccgc ccagggtctc ctgttgacgt gcttatccgt
      181 ccagatgaca ttgtgcatga cgatgcgagt cccttgcagg ccgttgtcct gaaaaagatg
      241 tttcgaggcg ccgacatttt gtacacgctg cgcctgcccg gcggcgcgac agtattatcc
      301 ctggtgccca gccatcacaa tcacccgatt gacgggaaaa taggtatcaa gctggaggtg
      361 gatcatgttg tggcattctg cccatcgaaa taaaaatcag gcatacgtac ctttcccctt
      421 catcgccttt cccccttacc acctttcgcc cctctttacc tccctgatcg cctttttcag
      481 atcacttccc tatggcaaga caccctgctt tgtgaaatcc gagtatccgc accgcgtatt
      541 cataaaaact gtcattattt tgcgtttccc tcttgaaatt cgaattcgac gatggacgac
      601 gaccgtaatt gccctgcgtg cagagaacaa ggaagtttcc tcatgctatc gttaatgaag
      661 ggcgcgtttt gcgcttccgg cacaaataaa atttgctgat atttaagggc ttatcgatcc
      721 ggatttgagg agaccgacat ggcgaagatg aacaagatac tcattgtcgt cactaacgcg
      781 gacgaattcg agaaagctgg ctacagaacc ggcttgtggc ttagtgagct aacccatttt
      841 tgggatgttg tgcaggaggc aggttatcaa atggatattg ccagcatatc aggcggtaaa
      901 attcccattg atcccgagag cctgattatc gctgaagttg ggcaggcggt tggaatggag
      961 ggcagcgttt ccaagcggta caaggataag gcgttcatga aactgctcga cgatacctta
     1021 agggtatcgg atgtggacgt ctcgacttat gatgccattt acatgacagg gggacatcgt
     1081 gtcatgtttg atttccccaa gagttccgcc ctggctgatc tggtggccag gttctacgaa
     1141 tccagcaaga ttgtttccgc cgtatgtcac ggaccctctg gtttactcga agcaaaatta
     1201 agcaatggta gttatctgat cgagaataag gatgtaactg gcttttcctg gaatgaagaa
     1261 gtgttggcca aacgcgacaa tgcagttccg ttcagtcttg aggaggagct gcaaaagaga
     1321 ggagccaagt acagcaaggc actattacct ttcaacgctc acgtcataga ggacggcatt
     1381 ttgattacag gccaaaatcc tggaagtgcc cgtgctgtcg gaaaggctgt cgttgggaag
     1441 ctcacggaaa tggcgcgatc gcgctgaagg catgtgcggg gagctagagc agttgtcttc
     1501 tggcccccga ttcccttgct atggtaaacc tgaatttctc gcgacccgga tttcttgcgc
     1561 cagctgcaat acagacatcg cataattcac gcttcgattg tagcgggtaa taacatagaa
     1621 gttattgaac ccgagccagt actcattccc gccctggttt tccagttcga tcactgtggc
     1681 ataaggatta cccgggacat tcacttgagg tgcgatgccc aacgtacgta catcggcaac
     1741 ggtataactg ggccagtttc cggtaaaaag tgcctcccgg tatttttcac tgtttaccag
     1801 cgcgggaact gctaccggca agccagcttc ccagccgtaa ctcttcagat agttggcaac
     1861 gcttcctatg gcgtccgccg tactgcccgc tagatcgatt ttgccatcat tgtcaaaatc
     1921 caccgcgtac ttgcgataac tgctgggcat gaattgcggg atgccgattg cgccagcgta
     1981 acttcccttc gtaccaaaca gatcagcgtt tttctcccgc tgcaacaaca ggtattgctc
     2041 cagttcgtcc ctgaaaaata cggcgcgcct ggggtaatcg aaagccaaag tggtcaaggc
     2101 atcaagaaca cggtgtttgc cggtctgcgc cccgtaaaaa gtttccaccc ccattattgc
     2161 cgcaatgatt tcctcgggaa tcccgtattc cctgcgtgcc cgccccagtg tttctgcgtg
     2221 ggcattccag aaagccaccc cccctgcaat gcgcttcgga gtaataaaca agagccgata
     2281 ttcatgccag ggttttgaag tagctggccg ggaaatcgta ttgataatcc cgggctgaaa
     2341 ctgggcctga cgaaggatgt tttccaggtc ggcctcaggg aacccgtggc gttcggccac
     2401 ttcgccaatg aattccctga tctcgggcct cagctcggac gcgggtattc ttgcgctcgt
     2461 cacgcatccc gcgagcgcaa aaaatccgat tgcacaagtc agtagacgca tgatttttcc
     2521 agagacggca actccgactt atccccttgc cacccggaat gagcgggcac tgtaactctt
     2581 cgtactttca ttcgcgcaat tgaatccaat aattttctcc ctcgactttt ctcatctata
     2641 tctcatcccc ccattcacgc caccctcgca gcaaggggaa ataaaggccc cgtctcaccg
     2701 attgaccgtt ctttcgcagc aagcctgcat aacggtcaag ctggctgcgg taacggtcct
     2761 gttcccggct aagaaagcct tccaggccgg ccccttcatg gctgcttgtt ttgtagtcca
     2821 cgacccaccg ctgaccactt gcatcacaaa aagtgcggtc aatcacaacg tcctgtggcc
     2881 gcccctcgat gattgccgtc atgcgcagct cgtttgcggc atcattctgg ggtccaagca
     2941 gccagcgccc tcgcgcatcg cttactgcat ggatcagtgc cttggcaacg cgccccgccg
     3001 ccaaatcgtc gtcattgttc tgatccattc cttcggccat taactgtttc cgaaagaccc
     3061 catgcagagc ctgaattcga gcaactgtcc agccctcaag ctcggtctcg gcaattattt
     3121 tcagccagcg atggacgacg ttcccgatgt ggcgagctgt ctcccccgcc cacgaatact
     3181 cgatatcaac gtgcatcgga tttgcgcgca tacctgcttt ccaccgcacc gccccgggcg
     3241 attccggcag ggcccaatca ggcgccaagc ggcgcagaga ttgatcaata gcgctatcgc
     3301 ccaattcttg agagcctgcg tgcaaactca aagaaactga tcgacatgaa tcctcataga
     3361 ccggttcggc gaccgtccag atttttttca gcagcgattt tctggccggt tccttgagct
     3421 cgtaccgccc gtccgggttg acggtaaggc ctgtgcttcc gaggagatgc aatcgcttcc
     3481 gggcacgcgt tgcagccaca tagagcaagc gctcatcctc aaaacgtgct ttttcgcagt
     3541 tcagcttttc cagccagtca taaatgcgat ccggctctcc acccgtttcc tggatgggag
     3601 cgagcaataa ttcatcgcct ctgctcacct catccttgcc tgaatcgcca tgcaagtttt
     3661 ccatccacat gaacagcttc ctggcatcgc tgcggggcga gcgcccgagg ccaggcagaa
     3721 tcaccgtatc gaactccagg ccctttgctt tatgtatggt catcacctgc agcaggccgt
     3781 cagccgagag atcgggtaat gcataaagcc gggtgagacc ttcttcaagt ccatccattt
     3841 ccatctcacc cgaattctcc cgcgcttcga ggtaatcaag atacgcttca gcgtcgccca
     3901 ggtccccctc actgtcgagg catgcaggac cccccaatgc gagccaggcc gcctcgactg
     3961 tgttgcggag agactggcgg caacggttgc cgatgcatac cttcagcacg tctcgaaggc
     4021 gggacagccg ggcacgacca tcaaccgaaa ccgcagccac gcgcgaatca tcattgagcc
     4081 actcccagac cgttcgatcg tttgcaactg cattttttcc ttcggaatca ttatcagccg
     4141 caatagcgga agtcgtcacc gatgtttccg ggcgccctgc cataagcgcg tggatatcag
     4201 cgagcgtgag cccgcaccac ggggcgcgca gcacggcgag ccaggctatg cggtccccca
     4261 gatggatcag ggcgcgcgta agagcgagca ggtcctgcac aacaggtcga aaagccaatc
     4321 cttcaatatc tatcgcacga aatctcaaac cggcatcccg cagctttggc gcgatctcgt
     4381 ggagatggct tcggttgcgt accagaactg ccactgagcc agagggatca tctgacctca
     4441 cgcgcttgac gatctctgtg accctgagcg cctcggcgaa gtgatcgtcg ttaaaaaatg
     4501 gatgtatggc tacggcaatg ccatcaaggg tctcccggac ggctactgaa ggtgtataag
     4561 gtgttgcccc aagcgtgacg tcctcatgct cgggtagaat gcgcgcaaaa gtgttgttca
     4621 cccattcgac gatcccgcgc tgtgatctga aatttgcgga taaggagacc tgttgtagag
     4681 gaatgttgcc cattcccgca gtgcgaacgc gaaggaagag gccgacttct gcttcccgga
     4741 accggtagat cgactgcatc ggatcgccga ccgccaggat gctccggcca tctcctggtt
     4801 cccatccggc agtaagtctc tggatgagct cgtactggct gatcgacgta tcctgaaact
     4861 cgtctatgag cagatgacgg acctggtagt cgagcgcgag tgcaagatcg gtgggaaggt
     4921 ccgcatcgcc gagggctcgc aacgccccct gcgccatctc gctgaaatcc actttattgt
     4981 gcgactggaa aacaagccgg agttgagcga ctgcatgcgg cagcagccgg atgatggcgc
     5041 ccagcacgtc ccattgattg tcgctgtatg agtgcggcgg cagctggcgg acatcgtgca
     5101 gtgcgagacg cagcaaatct ctggcgtcac ccgcggcaag cgactggata aggttgcacg
     5161 ctctttcctt ccattctttc gccgtatctt tcctgccctt tgttacggta ggaaaaccgt
     5221 cgttcaccgt gtgctgcttg cgccacattc cttcctttga caggagcagt ccagcgagcg
     5281 cacaccacgc gtcgaaatcc ttctcctctt ggccaggcag cgcatcgaac tggctaagct
     5341 gctcataatc cagcccgccc ccattcttgc cgcttgcggc caggttcccc gctgaatatc
     5401 gaagcaactc aacaagttct tcatgtataa aacgggggtg caactcaatc acctcgcgca
     5461 agcgtttgag cccgtcccgg cagatacgtc tcaacgcggc ctccagttcg tcacggtctc
     5521 ttccgtgaat gtggcgcagc cagtggtcgc ggcgcgccag catgccggca agcagcgatt
     5581 ggacacgggg cacatcgttg tctagatgct cgagcaatcg tcgcacttcg cttgctgcac
     5641 tcccatccgt ttcgatcaga tcgatggtcg cacttgccgc ggaaagatat aggccggagg
     5701 catcttcgac gatctccggc tggtgtccaa atcccgacaa aaccggcatc tgccggttca
     5761 gggaagcgca taatgaatcg aacgtcatga tccggaggcg gactggattt tcagcgagat
     5821 gccagcctgc ccgagtgtcg cgcgctgctg ctgcacttgc cagctcacgc atggtccgtt
     5881 catgttctgt ttgcgcccca gccggttgag cggattcgcg cgcgcgtgca agtgctttcg
     5941 tgacgcgctc gcgcatctcc ccggctgctt ttcgggtaaa tgtaatcgct acgatctcct
     6001 cgggctcgtt cacgtacgca agaagcttga ggtaacgctg gatcaaaagt cccgtctttc
     6061 cagatccggc cggcgcttga acgatgaaag actgggttgg atcaagcgca cggcgacgct
     6121 ccgcgaggtc tggaacagaa gaagcctgtg gggcttgagg catattgcct ggatgagccg
     6181 tgtcgggaat atagggtgaa ggtgcggtct gcgtgccgct cagcggattg ctcattcgct
     6241 ttcctcgtct tgcccaaacg cgcctccgac gcgctcatca attcggcaga gggagcgaaa
     6301 ttcgcagttt cggcacgtat gaggatattt cctgggattc atcctggcgc tgccagccga
     6361 aaaactggtc gcaatactga cgagatttgt tcgccaacca tcaaccagtt cgttccaaga
     6421 ggtgtgcttc acttttaacc gcggtcccgg aaatgcaacc gccccgggga gcaagtcgtc
     6481 atcgcgagcg agcgcgacaa agcggctatc gcccggttta acctgggcaa aggcaacggc
     6541 tacggcctcg gattcccccg cgacgagata caaaggcaat tgaggttcct ccggcctctc
     6601 gtcgagcatt gcagctgccg tcggcgcccg cgatttatag tcgattatga ctcgacgtcc
     6661 gtccgcgagt tcgtctaccc gatcgaggcg tgccgtcaac gcaagccctc cgatttcaat
     6721 ggttcgtttc tcttcaacag ccaccactga gaagtcggcc cgctttcgat cctggctcag
     6781 ccattcacgc gccaaccgta tgagccgtct acgctcgatc tcggaaaagt gtcgcgacat
     6841 tgcggcggga tgatcgcggc gcaagcgatc gattgcatcg tttgccgctc cccgaagcag
     6901 cacttcgagg tctggttcat ttatggcatc gagggcatct ttcgtcttga gcttgcgcca
     6961 cacctccgcc agcacgtggt ggatgagcgt gccgcgctcg gcgggtccga gcgcgaaggg
     7021 aggtctctca ggacgctccc cgcgtaatct gtacaacgcg aaagagcgga attggcatgc
     7081 cgcatgatct tttatgactg cagttccgcc gtgaacctcg gcactgaccc gggcatcagg
     7141 cgctatcctg agcgcaggca catcgctttc gtccagacgc tcaagcttgc gtgcctcatg
     7201 aatcagatcc cgataggccg ggtataaggg aggtgccagc gcgccagcgc tgatgctttc
     7261 gatcagcggg ctgaatgaaa gctcctgcgt ttcactgcct tggccgtgcc ggggatggct
     7321 gagaaccaga tcatccgcag ccccgagcca gtgatcggtg agcgtccttg ccagctcaag
     7381 ggtagcttgt gcagaactcc acggaacctc ccgttgcagc tcgacaggaa gaaatggatt
     7441 cggccgcgcc tgaggaggcc acgcttcgtt ggataatccc attacccaaa ggtgatcgaa
     7501 tgtcatgcct gcggattcga gcgcgcccag tatctgtatt ggaacatctg ctgtttccgg
     7561 ctggaagata acctcggcgg ccatgcggcg caggcgtgta accgcgtgtt tgcaggtcgt
     7621 gcgggagatg acggaatcga gggtagcgaa gtcggccaga acttcctgcc atttttttaa
     7681 cacctggtac tcgggtgaat cgagtcgccg ttctcccgga aaaccgatga gtcgaagtgc
     7741 ttcggcaatc ccgcgggcga gcgcggaagg tttttgcaaa ccacccagcg ctgtcttgct
     7801 gaagccgtga agcgccgcca aaatgcgcag gaggtcgggg cagcggattg cgttgcgctc
     7861 gcgttcgagc aaaaccagaa gccgatcgag cgttaccatc tcttctgccc gcttgcgcaa
     7921 ccgaatatcc agccaggcgc gttggatcat ttcaacctcg cccccactga tgaaaggcga
     7981 tcgaagcaga acgcttacct gttcaaacgc cacctttccc gtggaaagat cgagcaatag
     8041 aagcgcggca ttaaccagcg gataagagac aaggggctcg cccagcgaaa tattgaacgg
     8101 aagctccctg tccggctgcg caagcccttg ccggacgtca ggctccatca ctgcgctaaa
     8161 gattctgaag attgcgctgc ggcgcgcagc caaatccgga gctacaatcc cgatccgcgc
     8221 cgacggtacg gtctccaggt tcgcccgcgc ccattgggcg gctcgctgca tctcatcgcg
     8281 gacgtcatcg cactcaaggc gctgcagctt tcgtctggtg gtaaccggca gccgcggcgc
     8341 ctgcgcgatg accacctcac aacccacctc cttgagcctg gtcagcagcg ctgcatgctg
     8401 acgagtaacg atatcgaaac cgtagcatac cagcctcctg ggtttggcga tttccgggcg
     8461 tgcgagcaac tcgagcacaa gcccgccgac tcgcgcctta tccgtccggc gacgggccgt
     8521 ttcttcatac tgccgggacc attgacgaaa agctctcgca tcttcattca ggagatggcg
     8581 atcaagtcgc gggatgagat gccatgcatg ggcgagttgc caggcttcgc ttgccaggcg
     8641 agcagcttcc ccaacgtcaa gcagttcccg cccggagtct gagcaattaa tcgcatcctc
     8701 ccataacacc cgctcctgga taggggagag aagaacaggc aatgccgcgg cttccgacga
     8761 gtagagcgct tcactataga tacgctcgac gaatgccgag ataggtaaga tgtcggccga
     8821 attccaggcc gtcgcacttt tagcctggat tcggtcgaat tcgcttttaa ccgctcttgc
     8881 cagccgacgg ttgggcgtaa gtaccgtcag gctgccccgt gaaccggagg caagatcgtc
     8941 aaaaacgtca gaaaggggaa tagaaggaaa gtccgtttta gaggacaggc agggatatag
     9001 tgcgccgaag cgttgcccta gggacccgcg aagcgggacg gaaagcggag ggcgggcctc
     9061 gaccgcgttg tcatatgccg aggtgcctgc atcctcggca gctatgctgt ttcagcgttc
     9121 cagcttctcc atcttgtctg ttacctttgc ccactcatct gcttcgggta gagcgtcttt
     9181 tttctctata ataggcttcc
//
LOCUS       DASSHJ010000036         9754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_191657, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000036 DASSHJ010000000
VERSION     DASSHJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191657"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..165
                     /locus_tag="VFI43_01725"
     CDS             13..165
                     /locus_tag="VFI43_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060878.1"
                     /translation="MFAVGFPITLITGMTALMLSLPYFIPVLEQLVSKGLETMLEIAQ
                     ATRSAP"
     gene            547..1179
                     /locus_tag="VFI43_01730"
     CDS             547..1179
                     /locus_tag="VFI43_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060879.1"
                     /translation="MNEHLKILVTLLVTPLVTGCAQIKYFSVAPTTICPGEAVQVDWK
                     ASDRVLLSTLPPLPGTGEGPPEGSLSFTPVQDTRFTLKVPALLKSAQREWDVSVIPAQ
                     SSRLMGGVAQCSGNPASVSATFSVQQKDTSSRVRVTSIQNNYQRTLKVGKDNVEAEIA
                     PGTATDQFKNVPVSGTWTIRAPVAGGETCDSVLDSVRSRLTINVQMSCKE"
     gene            1185..2534
                     /locus_tag="VFI43_01735"
     CDS             1185..2534
                     /locus_tag="VFI43_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7060880.1"
                     /translation="MGGIDQLRTIVILMLENRSFDHILGHLSMPRFGNRKDVAGLVDP
                     EVNLDYTNFLDGQGYQPFELKDAPFAHDLPHSRGLVDVQLAKSGNRFTMSGFVDAYYQ
                     ATGSKVSDPPTMGFLSPADTPVSSFLAAQYAVCDNWFAPLPTDTQPNRSVAFSGYSLI
                     DDTKPRPIPTVQGSFVFEWLNAHNVSWRVYHCGISFFALFDRFEEVLGPAFRSFRQFP
                     GDWEAENVSEMPQVIFIEPEYTDSPVHLGWTPNDNHPPTAVGPGEHFVRSVYQVLTKD
                     PEKWKQTLLVLTNDEHGGFFDHVPPLPISAPVPAGSLFTIPFQSTGPRVPGIIASPWI
                     SAGSVFKGPMDHTSILQLLSEKFAGTPDYNDEVKRRREAGIQSVSQVLAEPLAQARED
                     IPALPEESISASVSLGPTVKPQTDSEQAFDRAARKLLEYDRKRALEKYPELVHLPEK"
     gene            complement(2546..3739)
                     /locus_tag="VFI43_01740"
     CDS             complement(2546..3739)
                     /locus_tag="VFI43_01740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7060881.1"
                     /translation="MELSNRVQAIKPSPTLAVTARAASLKAEGKDIIGLGAGEPDFDT
                     PQHIKDAAIAAINRGFTKYTAVGGTPGLKNAIVAKFRRENGFDYTPGQILVSCGGKQS
                     FFNLVLSVINPGDEVIIPAPYWVSYPDIALIAEGKPIIVEAGIEQGFKITPAQLEEAI
                     TLRTRMFVVNSPSNPSGAVYTLDELKALGDVLLKYPQILVATDDMYEHILLSGGRFVN
                     ILNACPGLYERTVVLNGVSKAYAMTGWRIGYCGGPAQIVAAMEKIQSQSTSNPTSISQ
                     VAAEAALNGDQSCIQPMVAAFKERNQFVTDSLNAIPGIRCLPSEGAFYAFSDVREVIA
                     RLNTSGLLNEASDLALSEYLLERGGVAVVPGSAFGSEGYIRLSFATSMDNLKNALGRI
                     AKVLS"
     gene            3876..5972
                     /gene="uvrB"
                     /locus_tag="VFI43_01745"
     CDS             3876..5972
                     /gene="uvrB"
                     /locus_tag="VFI43_01745"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7060882.1"
                     /translation="MIVTFPNSPYKLHQSFKPAGDQPSAIEKLIDGMEDGLAFQTLLG
                     VTGSGKTFTMANVIARLARPAIIMAPNKTLAAQLYAEMREFFPENAVEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLLERDDAIIVATVSCIYGIGDPVDYH
                     GMILHLREHEKITQRAAIQRLIEMQYERNEFEFARGTFRVRGDVLDIFPAENSETAVR
                     VSLFDDEVESMTLFDPLTGRTHKKVSRYTVYPSSHYVTPRSTTLRAIETIKAELRDRL
                     AFFQEHNKLVELQRLEQRTRFDLEMLNELGFCKGIENYSRHLSGKKPGEPPPTLIDYL
                     PRNTLMFIDESHVTVPQVGGMYKGDRSRKENLVDYGFRLPSALDNRPLRFDEFSKMMP
                     QTVFVSATPAEFERQHSGQVVEQVVRPTGLVDPKIEVRPASTQVDDLMSEVNLRTAKG
                     ERVLVTTLTKRMAEDLTDYFSDHGIKVRYLHSDIDTVERVEIVRDLRLGKFDVLVGIN
                     LLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHINGTALLYADRITGSMR
                     LAMDETERRRNKQSEFNQMHGITPRSVHKRVKDLIDGVYDMETAQQQLKAAQVRARYE
                     SMSEAELAKEIRLLEKQMLEAAKNLEFEKAAEYRDEIKKLRSKLFVGFVEPEAGDDQK
                     AARKGRVRKSTQKTVS"
     gene            6732..6896
                     /locus_tag="VFI43_01750"
     CDS             6732..6896
                     /locus_tag="VFI43_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060883.1"
                     /translation="MALQLTTRTIDVEQRESALVVAADVAARRHWAILIDGGLLRYAE
                     TIHSFIDPQG"
     gene            7250..8257
                     /locus_tag="VFI43_01755"
     CDS             7250..8257
                     /locus_tag="VFI43_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060884.1"
                     /translation="MALQLTIHTTDIGPGESALVVAADVAAGRHRTILIDGGLLGYAE
                     TVRNFINPILRGYGSPLDRILVSHYDKDHSGGVIGLLNADNMRVVSEAISNAVAKAVQ
                     WAAAGIRPQQIACGALAASATILGACGLNAEQILTMCQVVFPLAVGKTDDEAAEIGSN
                     YALEHINKLQTFPTLPVGETGRGKNIAKISSIAASIAIASGNNPATAAFGAIFLLIAR
                     TLPNQPRFDTGGRYCNVHLIDIGQSVWPSQSAEISADAATGQCVTKTNRMIASGGATP
                     APGDEIPWSTRPDPAPAPAHSPETFCLAINSWVWQGIGNNPVQTVWRRKAGGRMSKRA
                     F"
     gene            8414..>9754
                     /locus_tag="VFI43_01760"
     CDS             8414..>9754
                     /locus_tag="VFI43_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7060885.1"
                     /translation="MNQIVIVALRKPYTFVVMSILIVIFGIRAIRHSPTDVFPTIKIP
                     VISVVWAYAGMLPTDVSGRITFFFERFLTSTVEGISDIVSQCYYGISITNIFLQPHTN
                     LPGAEAEVTAIAQTIVKALPPDISPPMIMRLEASQVAVASLQVTSDEMTPADLYNLSY
                     NQLRPLIVVIPGAIVPHPYGGKPKQLLVSLDQNKMLARNISPTDVHAAFDRQNMVLPA
                     GDMKIKQTDWMVQTNAMPPEIPEFNDIPIKREGNSFVFLRDVADVQLAGPPQTNSVMV
                     DGKQAVIIVVMKSGEASTLDVVDGIRKTLPRIKEIVPPHVDVRIITDASVFVRESIDE
                     VMHEMMIAGLLVGFIVMLMLGSWRPMIVVTTSIPLCILTSLICLQVLGESMNVMTLGG
                     LALAVGILVDDATVMIENIDTHLEMGKELDEAIIDAANQIVIPTLLATLCICIVW"
BASE COUNT         2286 a         2652 c         2595 g         2221 t
ORIGIN      
        1 cctcagctca atatcttcgc cgtgggtttc ccgatcacct tgataacggg catgaccgcg
       61 ctgatgctat ctctgcccta ttttattccg gtactcgagc aactcgtttc gaaagggctg
      121 gaaaccatgc ttgagatagc acaggcaaca cgcagcgcgc cttaacgctc cagctctttc
      181 gatgcaggtg gcaaaaggct ggagcagcca tgcgaaatgc tgaaatctcc gccgtgtggc
      241 atcctattgc acttaacgaa attgctgcta cagtgtaata gaagctgaac gttgttctcc
      301 cggctcgctc gtttgagccg tctcttcact ctttccagag gtcggcatgc cccgtcaatc
      361 ctgctatttt cattgattgc ggcagacact cgctgacccc agagcaaccg ggatatctga
      421 tacttgaata ccattttcgc cgcatttaca acatcatcat ctttttgctg atttatcaaa
      481 acagaggagc aggcgaggtg tttcccgtca ggcaaaaggt tttaggaagg cgatgagatg
      541 caccggatga acgaacatct caagatcctg gtgacgctac tcgtcacccc gctggtcacg
      601 ggctgtgccc agatcaaata cttctccgta gccccgacga cgatatgccc cggagaggcg
      661 gtccaggttg actggaaagc ttcggacagg gttttgctga gcacgcttcc tccgcttccc
      721 ggaacaggag aaggtcctcc tgaaggatcg ctatccttta ctcccgtgca ggacacccga
      781 ttcacactca aggtgccggc attgctcaag agtgcgcagc gggagtggga cgtctcggtc
      841 attcccgcgc aaagttcccg gttgatggga ggcgttgccc agtgcagcgg aaatcccgct
      901 tcggtatcgg caactttttc tgtccagcaa aaagatactt cctcacgtgt ccgggtgacc
      961 tccatccaga acaattacca gcggacgctc aaggtcggta aggataacgt cgaggcagag
     1021 atcgcgcctg gtacggcaac cgaccagttc aagaatgttc ccgtttcggg aacatggaca
     1081 attcgtgcgc cggtagccgg cggcgagacc tgtgattcgg ttcttgactc ggttcgcagc
     1141 cggctgacga tcaacgtgca gatgagctgc aaggagtgat ggtgatgggt ggaatcgatc
     1201 agcttcggac aatcgttatc ctgatgctgg aaaaccggtc attcgaccat attctcggac
     1261 acctcagcat gccgcggttt ggaaaccgga aggacgtcgc aggacttgtc gaccctgagg
     1321 tgaacctcga ctatacaaat tttcttgatg gtcagggcta tcagccattt gaactgaagg
     1381 acgcgccctt tgctcatgac ctgcctcata gccggggtct cgtggacgtc cagctggcaa
     1441 aaagcggtaa ccggtttacc atgagtggtt tcgtcgacgc atattaccag gcgaccggaa
     1501 gcaaagtgag cgatccgccg acaatgggat tcctctcacc cgccgatacg cccgtgtcaa
     1561 gctttctggc tgcgcaatac gcggtctgcg acaactggtt tgccccgctt ccaaccgaca
     1621 cccagcctaa ccggtcagta gcattcagtg gctattcatt gattgatgat acgaagcctc
     1681 ggcctattcc caccgtacaa gggagcttcg ttttcgagtg gttgaatgct cacaatgtca
     1741 gctggcgggt ctaccactgc ggaatatcat tttttgcgct gtttgaccgg tttgaagagg
     1801 tgctgggtcc tgccttccgc agcttcaggc agtttcccgg cgactgggaa gccgaaaacg
     1861 tgtccgagat gccgcaggtc atcttcatag aacccgagta tacggattcg cctgttcatc
     1921 tcggctggac gcctaatgac aatcatccgc ctacagccgt tggccctgga gagcactttg
     1981 tgcggagcgt ttatcaagtg ctgaccaagg atccggaaaa atggaaacag accttgctgg
     2041 tgctcacgaa tgacgagcac ggagggtttt ttgaccacgt tccgccattg cccatttctg
     2101 cgccggtacc cgcagggtcc ctgtttacga tcccctttca gagcaccgga cccagagtgc
     2161 ccggcataat tgcctcaccc tggatttccg ccggctccgt gttcaagggg cccatggatc
     2221 atacgtccat cctgcaactg ctgtcggaga agttcgcggg aacaccggac tataacgacg
     2281 aggtaaaacg gcggagagaa gccggaatac aaagcgtctc gcaggttctc gccgaaccgc
     2341 ttgcccaggc gagggaggat atccctgcgc ttcctgaaga gagtatttct gcttctgtca
     2401 gcctggggcc aacagtgaag cctcaaacag atagtgaaca ggcatttgac agggcggcgc
     2461 ggaagctttt ggaatatgac cgcaagcggg cgctggaaaa ataccctgaa ctcgtacatc
     2521 tgcccgagaa atagcttcta ctttttcagg acagcacctt cgcgatcctg cccaaggcgt
     2581 ttttcaggtt gtccatgctc gtggcgaagg aaagccgtat gtatccttcg ctcccgaacg
     2641 cggaacctgg cactactgca actcctcccc gttccagcag atattcgctc aaggcaagat
     2701 cgcttgcttc attcagcagt ccgctcgtat tcaatctcgc gataacttcc cggacgtctg
     2761 agaaggcata gaaagcacct tcagaaggta ggcacctgat gccgggaata gcattgaggg
     2821 aatcggttac aaattggttc cgctccttga aggcggcaac cattggctgg atgcacgatt
     2881 gatcgccgtt aagggcggct tccgcagcaa cctgggaaat ggacgtggga ttggacgtgc
     2941 tctgggattg aattttctcc atggccgcca cgatttgtgc cggaccgcca cagtaaccga
     3001 tgcgccagcc tgtcatcgca tacgccttgg agaccccgtt cagcacgact gttcgctcgt
     3061 aaaggccggg acaggcgttg agaatgttta cgaaccggcc cccggaaagc aggatgtgtt
     3121 cgtacatgtc atcggtcgca accagaatct gcgggtattt tagcaaaacg tcccccagcg
     3181 ccttgagctc gtccagtgtg taaacggcgc cggaaggatt gctggggcta ttgacgacaa
     3241 acatccgggt gcgcagcgtg attgcttctt cgagctgtgc tggcgttatt ttaaaaccct
     3301 gctctatacc ggcttcaaca ataatcggct tcccttcggc aatgagagcg atatcaggat
     3361 aggaaaccca atagggggcg ggtatgatga cttcatcgcc agggttgatg acggaaagta
     3421 caaggttgaa gaaactctgc tttccgccgc atgacacgag aatttgcccg ggcgtgtaat
     3481 caaatccgtt ttcacgtctg aacttcgcta cgattgcatt cttcaggcca ggcgtcccgc
     3541 cgaccgcggt atattttgta aaccccctgt tgatggcggc gatggccgca tccttgatgt
     3601 gttgcggcgt gtcgaaatcc ggctcgcccg cgcccagccc tatgatgtct ttgccttccg
     3661 ccttcaggct ggctgccctg gcagtaacgg ccagggtagg ggagggcttg atggcctgaa
     3721 cacggtttga aagttccacg ataaaagtcc ttgcgccggt agaggagcgc gtgcatgata
     3781 aaatggcttg atcgaatacc cgttaacggt caagcagtag gtagctgaac ccgaaattat
     3841 actgtgatcc ttgccaatag tgatctttac caatagtgat cgttaccttc ccaaatagtc
     3901 cttataagct ccaccagtcg ttcaagcccg ccggggatca gccaagcgca atcgaaaaac
     3961 tgatcgatgg gatggaggat ggcctcgcct tccagacatt gcttggcgtg acggggtccg
     4021 gcaagacgtt cacgatggcg aacgtcattg cacggcttgc gcgccccgcc atcatcatgg
     4081 cccccaacaa gacactggcc gcgcagctat acgcggagat gcgcgagttc tttcccgaga
     4141 atgccgtcga atacttcgtg tcgtattacg attattatca gcccgaagcc tacgttccgt
     4201 cgcgcgatct tttcatcgaa aaggattcca gcataaacga gcatatcgag cagatgcggc
     4261 tctctgcgac caaatccttg ctcgaacggg atgatgcaat catagtcgcc actgtttcct
     4321 gtatatacgg aatcggcgat ccggtcgatt atcatggaat gatcctgcac ttgagggagc
     4381 acgaaaaaat aacccagcgt gcggccattc aacgcctgat cgaaatgcaa tatgagcgca
     4441 acgagttcga atttgcacga gggactttcc gggtgagggg agatgtcctg gatatatttc
     4501 cggcggagaa ttcagaaacg gcggtcagag tttccctgtt cgacgacgag gtcgaaagca
     4561 tgaccctttt cgatcctctc accggccgca cgcacaaaaa ggtatcgcgt tacacggttt
     4621 atccttcgag ccactacgtc accccgcgaa gcacgacttt gcgggcgatc gagacaatca
     4681 aggcggaatt gcgtgatcga cttgctttct ttcaggaaca caacaagctg gtcgaactcc
     4741 agcggctcga gcagcggacc cgctttgatc ttgaaatgct caatgagctt ggattctgca
     4801 aaggcatcga gaattattca cggcatcttt ccggaaaaaa accaggagaa ccaccgccta
     4861 ccctgatcga ttatttgccg cgaaatacgc ttatgtttat cgatgaaagc catgttaccg
     4921 ttccccaggt cggagggatg tacaaaggag atcgctcccg aaaggaaaac ctggtggatt
     4981 atggctttcg gctaccctcg gccctcgaca accgcccgtt gcggttcgat gaattcagca
     5041 aaatgatgcc gcaaaccgta tttgtttcgg caaccccggc ggagtttgag cgccagcaca
     5101 gcggccaggt cgtggagcag gtggttcggc cgaccgggct ggtggatccg aagatcgagg
     5161 ttcgcccagc atcgacgcag gtggatgacc tcatgtcgga ggtcaatttg cgtactgcga
     5221 aaggtgagcg cgtgctggta acgacgctga ccaagcggat ggccgaggac ctcaccgatt
     5281 acttttcaga ccatggtata aaggtacgtt atcttcattc cgatattgat actgtggaac
     5341 gcgtcgaaat tgtgcgcgac ctgaggttgg gaaaattcga tgtactcgtt ggaatcaatt
     5401 tgctgcgcga aggactcgat attccggaag tatcgcttgt agccatcctc gatgcggaca
     5461 aggagggatt cctgcgttcg gaacgttccc tgatccagac cattggacgg gcggcccgcc
     5521 acatcaacgg gactgcgctg ctatatgccg accgcattac cggctccatg cgtcttgcaa
     5581 tggatgagac agagcgccgc cgaaataaac agagcgagtt caaccaaatg cacggcatca
     5641 ccccgcgcag cgtccacaag cgggtcaagg acctgattga cggggtttat gacatggaaa
     5701 ccgcgcagca acaactcaag gccgcccagg ttcgagcccg ctatgagtcg atgagcgaag
     5761 ccgagcttgc aaaagagatc aggctattgg aaaagcagat gctcgaggcg gcaaaaaatc
     5821 tggagttcga gaaagcggcg gagtaccggg atgaaataaa gaaactcagg agtaaattgt
     5881 tcgtggggtt cgttgaacct gaagctggcg atgatcagaa agcagcccgg aaaggcaggg
     5941 tacgaaaatc gactcagaaa acagtgtctt gaaaggtgtt tttagcagtc cctctttttt
     6001 atagttgcag agatagcagt ctgccgggat ttgtaatttt gttcccgcaa agttcacgca
     6061 gttgtcccat ccgactgtgc gcccgaatcg ctcgcagagg gtgccatcaa gatcactacc
     6121 aatccaggca tgaaggcatt gcgatcggat cttgtccgtt gcatgaactc ggcattgaaa
     6181 ttgctgggcc ttcaacctct cgcattccca acactttccc gtcagggcaa cagctttctc
     6241 cccactcccg tcgcctccag ggcgagacga ggagagtacg agcgagagga tgtattttta
     6301 agagcaactt tctttaaatc ccgtaccaaa agaacccgac ctgcccctta ccgttaatat
     6361 ctcgggccac gatgtaaata tcgcctgcca aggtcttaca tacccatcct cggggaccgg
     6421 tgataaaaca atgagtgttt ccagtggtga aagacagact ctcctgcttc ttctccagag
     6481 gagaaacctg cgcttcggtc agcatttccc cctctcaccg agctctacag ccgccaacgc
     6541 ttcttgagcg atcaggtcga gtattcacaa attgatcact tatacatcac actaccataa
     6601 cacgggttca caaattgacc acttatatac gatattaccc caacaaggag tgtagtaatg
     6661 tgatttcaac ttggccacgc tctcgggtgt gtgaattact tgtttatctc cgaaggaaaa
     6721 acttgcagct catggccctg cagctcacca cccgtacgat tgacgtcgag cagcgagaaa
     6781 gtgcgctcgt tgttgcggca gacgtggcag ctaggcggca ctgggcgatt ttgatcgatg
     6841 gcgggctact ccggtatgcg gagacaatac acagctttat tgatcctcag gggtgaggtt
     6901 accccctcga tcacattccc gtcagccact gcgacaagga cctcagggtg agtcgtaaga
     6961 ccctgcatgc gaacaatatg cgagtcgaaa gcgaggcgga acccttcgct tcggtcagca
     7021 tttccccctc tcatcgcagc gccgcagccg ccgatgcttc ttgaatgctc aggtctagta
     7081 ttcacaaatt gactacttat acatcacatt accgtaacac ggattcacaa attgaccact
     7141 tatacatgat attaccgtta caagggctgt ggtaatgtga tttcaacctc gccacgctcc
     7201 cgcgtgtgcg aattgcttgt ttatcttgga agggaaaacc tggaagccca tggccctgca
     7261 actcaccatt catacgactg acatcggtcc gggagaaagt gcgctcgttg ttgcggcaga
     7321 cgtggcagcc gggcgacacc ggacgatatt gatcgatggc ggactgctcg ggtatgcgga
     7381 gacagtccgc aactttatca atccaattct caggggttac ggttcccccc tcgatcgcat
     7441 tctcgtcagc cactacgaca aggaccattc agggggagtc ataggactcc tgaatgctga
     7501 caacatgcga gtcgtaagcg aggccatctc gaacgcagtg gcaaaggcgg tgcaatgggc
     7561 cgcagcaggc attcgcccac agcaaatcgc ctgcggagct ttggccgcat cggcaacaat
     7621 cctcggagct tgcggcctga acgcagagca aatcctgaca atgtgccaag tcgtttttcc
     7681 cctggccgtc gggaagacag acgacgaggc cgcagagatc ggatctaact atgccctgga
     7741 gcatattaac aagcttcaga cctttcccac ccttccagtc ggtgaaaccg ggagaggtaa
     7801 aaatatcgca aaaatatcct cgatcgctgc gtcgattgct atcgcgtcgg gaaacaatcc
     7861 cgctactgca gcgtttggcg ccatcttcct tttgattgcc agaacacttc ctaatcagcc
     7921 ccggttcgac accgggggcc gatactgtaa cgtgcatctt atcgatatcg gtcaatcagt
     7981 ttggccatca caatctgccg agatatccgc cgacgcggcg accgggcaat gcgtaacgaa
     8041 aacgaatcgg atgatagcgt ctggcggggc gacgccagcg ccaggggacg agattccctg
     8101 gagcacgcgg cccgatccgg ctcccgctcc cgcccattct cctgaaacat tctgcctggc
     8161 gatcaactcc tgggtttggc aagggatcgg caataaccct gttcagaccg tctggagacg
     8221 caaggctgga ggccgcatgt ctaaacgcgc gttctaataa tttccgctga caattccttt
     8281 ctttatgcgg cgtggacaat gaattgtctt ataaaggttt gcaaaagaat cgcttataaa
     8341 agagttgcct cacccttaca tgcaatattg tagtaagtga attttttacc gaaggtataa
     8401 gaggaaacag agtatgaacc agattgtgat cgttgcgtta cggaaaccct acaccttcgt
     8461 ggtcatgtca attctgatcg tcatattcgg aatcagggcc atacggcact cgccgacgga
     8521 cgtctttccc actatcaaga ttcccgtgat ctccgtagtc tgggcttacg ccggcatgtt
     8581 gccgacggat gtgtcgggcc gtatcacttt tttcttcgaa cgcttcttga cctcgacggt
     8641 ggaaggtatc tcggacatcg tcagccagtg ttactacggc atcagcatca cgaatatttt
     8701 tctacagccc cacaccaatc tccctggcgc agaagcggaa gtcacagcca tagcccagac
     8761 gattgtcaag gcgctgccgc cggatatttc gccgcccatg atcatgcgcc tggaagcctc
     8821 tcaggtagcg gtagccagcc tgcaggttac ctcggacgag atgacgccgg cagatctcta
     8881 taatctttct tataaccaac tcaggcccct gattgtcgtg atcccggggg caattgttcc
     8941 gcatccttac ggtggaaagc ccaagcagct tctggtatcc ctcgatcaga acaagatgct
     9001 ggctcgcaat atatcgccaa ccgatgtcca tgcggctttc gatcgccaga atatggtgct
     9061 gcccgcgggc gatatgaaga tcaagcagac cgactggatg gtccagacaa acgcgatgcc
     9121 gccggaaatt ccggaattca acgatatccc gataaagcgg gaaggaaatt cgttcgtttt
     9181 tttgcgtgac gtcgccgatg tccagctcgc ggggccaccg caaacaaact ccgtcatggt
     9241 ggacggtaaa caggctgtca ttattgtcgt catgaaaagc ggcgaggcat cgaccctgga
     9301 cgtggtcgac ggcatcagga aaacccttcc gcgcatcaag gagatcgtgc cgccccatgt
     9361 ggacgttaga atcatcaccg atgcgtcggt cttcgtgagg gaatcgatag acgaggtgat
     9421 gcatgagatg atgatcgcgg gcctgcttgt tgggttcatc gtcatgctca tgctcggctc
     9481 gtggcgtccc atgatagtcg ttacgacttc cattccgttg tgtattctga cctccctcat
     9541 ctgcttgcag gtgctgggcg aatcaatgaa cgtcatgacc ctggggggat tggcgctggc
     9601 ggtagggatt cttgtcgacg acgcgacagt gatgatcgag aacatcgata ctcacctgga
     9661 aatgggcaag gaactcgatg aggcaatcat cgatgcggca aaccagatcg ttattccaac
     9721 gctgctcgcc acattatgca tatgcattgt ctgg
//
LOCUS       DASSHJ010000037         9714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_956642, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000037 DASSHJ010000000
VERSION     DASSHJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956642"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            619..1560
                     /gene="pstC"
                     /locus_tag="VFI43_01765"
     CDS             619..1560
                     /gene="pstC"
                     /locus_tag="VFI43_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7060886.1"
                     /translation="MPLPATQQPAGDGEPRKLGYRYSRHLGERAIEGILFLTAFASVA
                     ITVAIVVMLVRESLIFFEHVPLWNFLTDTQWTPLFDDAHYGILPLVSGTLVSSAVALG
                     VAIPLGTITAIYLSEFAPFAVREIAKPFLELLGGVPTVVYGYFALLFVTPLLQKVLPD
                     LPGFSLLSAGLVMGIMIIPYVSSMSEDAMRAVPMNLREGSYAMGATRFQTAIRVVMPA
                     AFSGIAAAYILGISRAVGETMVVAIAAGMQPNLTWNPLEPAATITAYIVQVSLGDLPH
                     GSIGYQTIFAAGLTLLLLTLTFNIIGHVLRKRYREVY"
     gene            1633..2559
                     /gene="pstA"
                     /locus_tag="VFI43_01770"
     CDS             1633..2559
                     /gene="pstA"
                     /locus_tag="VFI43_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7060887.1"
                     /translation="MNDERDLGEIRGIIARHKLWDLIFMIVGIVVLMIAVLTFMALFS
                     HMLIEGLPRLSWDFFTSFPSRRPASAGILSAWVGTTLVMLVTAIAAVPMGIGAGIYLE
                     EYGPKNLITEIIEINVSNLAGVPSIIYGLLALGLFVHQLGFGQSILSAGLALALLILP
                     VVIVATREAIRAIPVGIREGAYALGATKWQTVSDHLLPYSAAGIMTGVIIGLARAIGE
                     TAPIITIGALTFIAFLPPSPITPEFPFISFDWLMAPFTVMPIQMFNWISRPEEEFHIN
                     AAAAGLVLVMMTLTMNALAITLRYRMRKNLKW"
     gene            2580..3365
                     /gene="pstB"
                     /locus_tag="VFI43_01775"
     CDS             2580..3365
                     /gene="pstB"
                     /locus_tag="VFI43_01775"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET7060888.1"
                     /translation="MPVAPSAQPRYKSEVRNLSFYYGSFSALKNINMLLHERQVTALI
                     GPSGCGKSTFLRCFNRMHDLYPGNRYVGEIILYPDNVNVLSRNVDPIEVRMRISMVFQ
                     KPNPFPKSIYENVAYGLRVRGVRKRAILDEKVEEALRGAALWDEVKDRLNQLAFNLSG
                     GQQQRLCIARALATDPEILLFDEPTSALDPIATASIEELIADLKDKVTILIVTHNMQQ
                     AARVSDYTAYMYLGEVIEFDVTDTIFIKPKNKQTEDYITGRFG"
     gene            3626..4378
                     /gene="tpiA"
                     /locus_tag="VFI43_01780"
     CDS             3626..4378
                     /gene="tpiA"
                     /locus_tag="VFI43_01780"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7060889.1"
                     /translation="MRSKLVAGNWKMHGETVQNQALLSAILDSARDLDGMETAVCVPY
                     PYLQQVQTALQASDISWGAQNVSQYDKGAYTGEVSAGMLMDFGCRYVIVGHSERRALY
                     GEDSRTVALKFKAAQAAGLTPILCVGETLDQREAGITERVVAEQLDAVSELAGVEALA
                     KSVLAYEPVWAIGTGKTATPGQAQDVHTFIRNRIAARNPEVGGSLKILYGGSVKAGNA
                     GELFAMPDIDGGLVGGASLVADEFLAICRAAK"
     gene            4396..4746
                     /gene="secG"
                     /locus_tag="VFI43_01785"
     CDS             4396..4746
                     /gene="secG"
                     /locus_tag="VFI43_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7060890.1"
                     /translation="METIVWILHILSAVSVIVLVLLQHGKGADMGAAFGSGSSGSLFG
                     ASGSANFLSRTTGVLAGVFFITSLGLTYLSSHKIEGGGVMDKEVPIQAPKAAEQAPEI
                     PSDGGASKATEIPK"
     gene            4825..4909
                     /locus_tag="VFI43_01790"
     tRNA            4825..4909
                     /locus_tag="VFI43_01790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4859..4861,aa:Leu,seq:gag)
     gene            4991..5347
                     /locus_tag="VFI43_01795"
     CDS             4991..5347
                     /locus_tag="VFI43_01795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET7060891.1"
                     /translation="MLENYFPILLFIFVGLAVGVVPMLLGRMFGPNRPDSEKLSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLNEIGLFGFIAMVVFLGIL
                     IVGFIYEWTKGALEWD"
     gene            5351..5827
                     /locus_tag="VFI43_01800"
     CDS             5351..5827
                     /locus_tag="VFI43_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET7060892.1"
                     /translation="MSANGGVEKGFITTSLDNVINWGRTGSMWPMTFGLACCAVEMMQ
                     AGASRYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGTC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIHRTNTIAR"
     gene            5834..6451
                     /locus_tag="VFI43_01805"
     CDS             5834..6451
                     /locus_tag="VFI43_01805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET7060893.1"
                     /translation="MSSSRLEALSFCLQNVLPERLAGIRERLGELTITLRPSDLLGVM
                     QTLRDHSDLAFEVLIDLCGVDYSTYGAVSVLDNERKGQRFAVVYHLLSIKHNTRLRVR
                     TFAEDDELPVVDSVIDIWPVANWFEREAFDLFGIVFKGHPDLRRILTDYGFIGNPFRK
                     DFPLSGNVEMRYDPDQGRVIYQPVTIEPRQIIQRVIREEHYGDSE"
     gene            6520..7773
                     /locus_tag="VFI43_01810"
     CDS             6520..7773
                     /locus_tag="VFI43_01810"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET7060894.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAESKSFIQSVPYMDRLDYVSMMCNEHAYVMAIEKLLQIEVPLRAQYVRVMFD
                     EITRILNHLLWLGAHALDVGAMTVFLYAFRDREDLMDCYEAVSGARMHAAYYRPGGVY
                     RDLPDSMPQYQPSRIRDEKSTALRNENRQGSLLDFIEDFTNRFPGYVDEYETLLTDNR
                     IWKQRLVGIGVVSPERAKALGFTGPMLRGSGVEWDLRKKQPYEVYDRMDFDIPVGVNG
                     DCYDRYLVRIEEFRQSNRIIKQCVDWLRKNPGPVITDNHKVAPPPRLSMKKNMEELIH
                     HFKLFTEGIHVPEGEAYAGIEHPKGEFGIYLISDGANMPYRLKIRAPGFAHLAALDEM
                     SRGHMIADVVAIIGTQDIVFGEIDR"
     gene            7811..8287
                     /gene="nuoE"
                     /locus_tag="VFI43_01815"
     CDS             7811..8287
                     /gene="nuoE"
                     /locus_tag="VFI43_01815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET7060895.1"
                     /translation="MLSAESLQKIDREMAKYPADQKQSAVMSALAIAQDEKGWLATET
                     MDFVARYLGMPPIAVYEVATFYNMYNLQPLGKYKITVCTNLPCALSGANGAVARIRQR
                     LGIGFNETTTDNRFTLKEGECMGACGDAPVLLVNNKRMCSFMSDAKIDELLDELSK"
     gene            8284..9561
                     /gene="nuoF"
                     /locus_tag="VFI43_01820"
     CDS             8284..9561
                     /gene="nuoF"
                     /locus_tag="VFI43_01820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7060896.1"
                     /translation="MSQPTQILLAGLDPSDPDNWRLKSYEQREGYAALKKILNEKIPP
                     EKIIEEVKKSALRGRGGAGFPTGLKWSFMPKQYEGEKYLVCNSDEGEPGTFKDRDIMR
                     HNPHILIEGMLIGAYAMGIRAGYNYIHGEIWDVYERMEEAVDEARAAGYLGNNILGSD
                     FSFQLHNHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETF
                     ASVPWIIRNGGEKFLQLGKPNNGGTKLFSVSGHVNKPGNYEIPLGTPFATLLEMAGGM
                     RGGRKLKGCIPGGSSMPVLPGDVMMQTDMDYDSIAKAGSMLGSGAVIIMDDTTCMVRA
                     LERLSYFYFEESCGQCTPCREGTGWLYRVVHRIETGKGRMEDLDLLNNVADNIQGRTI
                     CALGDAAAMPVRAMINHFRDEFAYHIEHKKCLV"
BASE COUNT         2202 a         2526 c         2679 g         2307 t
ORIGIN      
        1 acctgcatcg attgccacca gaacctggtg cacaaggaag tggccgagac cgacctgaac
       61 gaaagcaaga aggcaggcaa gatggtcctc aagcccgaaa agaaatccaa ggatgaggac
      121 gaggacgacg aggaagagta ataatagctg gagcctttga cgaagcgacg agggtgcggc
      181 agaaaacgga taactacgaa tgccccgatc tcgccatcac gtcgaatcaa ccagcgattc
      241 atcgtccttc ggaagtaact gccgctttcg gtcgacctcg cacccaaatg attcaatgat
      301 tcgctgatct gccccttcag gtctggttac caggcctgga ggggcagatc acgttttaac
      361 ccgctgccgg gcctacccga ccttcaccca gcttttggtt gctggatgaa acccatgcgg
      421 aatgtgcgct atccaacgct acaggcttgc gtattggaaa agttcaagcg agaattcttc
      481 cactacccgt tctcgtttcc cttggggaac gttagaatgg cacgtcagtc tgggaaacct
      541 ccgcaccctc actcatagct gtcacgaggc tgtaacaaat ccatcataat atctcctgct
      601 caggaacagc cccctagaat gcctttacct gcaacccaac agcctgctgg agacggcgag
      661 cccagaaaac tcggctaccg atattcgcgt catctgggtg agcgggctat cgagggtatt
      721 ctgtttctaa ccgcatttgc ttctgttgca ataacagtcg cgatcgtcgt gatgctggta
      781 agggaatcgc tcattttctt cgagcatgtt ccactttgga atttccttac tgatacacaa
      841 tggacaccat tattcgacga tgcgcactat gggatactcc cccttgtttc cggtaccctc
      901 gtaagctctg ccgtcgcgct tggtgtcgcc attccgctgg gcaccataac tgccatatac
      961 ctgtccgaat ttgcgccatt tgctgtacgg gaaatagcca agcccttcct tgagctgctg
     1021 ggtggtgtgc ctacggtcgt atatggctac ttcgcgctgc tgttcgtcac cccgttgcta
     1081 cagaaagttc tgccggatct gccgggtttc agcttactgt cggcgggcct ggtaatgggc
     1141 atcatgatca ttccctatgt cagctcaatg tcggaagatg ccatgcgggc ggttcccatg
     1201 aatttgcgcg aggggtctta tgcaatggga gctacccggt tccagacggc catccgggtg
     1261 gtaatgccag ctgcattttc cggcattgcc gccgcatata ttctcggtat ttcccgcgcc
     1321 gtcggagaaa cgatggtggt agcgatagca gcgggcatgc aacccaacct tacctggaat
     1381 ccgctcgaac cggcggccac cattaccgcc tacatcgtcc aggtcagcct gggggatctg
     1441 ccccatggca gcattggtta tcaaacaata ttcgcagcag gactgacatt gctgctattg
     1501 accttgacat tcaacatcat cggtcatgtc ctgcgcaagc gctaccggga agtctattaa
     1561 ggtcctctgg ctaacccagg gtgaaaggat ttccgtgatc tccagcagaa ccagcatgaa
     1621 aagaggcgat cgatgaacga tgagcgggac ctgggcgaga ttcgcgggat cattgcgcgc
     1681 cataagcttt gggatctgat tttcatgatt gtcggcatag tcgttttgat gattgccgtc
     1741 ctgaccttca tggccctgtt ttcccatatg ctgatagaag gattgccgcg cctctcctgg
     1801 gatttcttca cttcttttcc ttcccggcgt cctgcctcag ccggtattct ctccgcgtgg
     1861 gtgggtacta cgcttgtcat gctggtgacc gccatcgcgg ctgtgcccat gggaattggc
     1921 gcgggtatct acctggagga atacggccca aaaaatctca ttaccgagat catcgaaatc
     1981 aacgtctcaa accttgccgg ggttccatcg attatttatg gcctgcttgc attagggctt
     2041 tttgttcatc aactcggatt tgggcaaagt attctttccg ctggccttgc gctggctttg
     2101 ctcatccttc ctgtagtaat tgtcgcaacc cgggaagcaa tacgggccat tcccgtgggt
     2161 attcgcgagg gcgcttatgc gcttggcgca acaaaatggc agacagtctc agatcatctt
     2221 cttccctatt ctgccgcagg tatcatgaca ggggtgatca tcgggcttgc ccgagcgatt
     2281 ggagagacag ctcccatcat tacaatcggt gcattgactt ttattgcgtt tttgccacct
     2341 tctcccataa cgcccgagtt cccgtttata tcttttgatt ggctgatggc gccctttacc
     2401 gtcatgccca tccagatgtt taactggata tcgcgacctg aagaagaatt tcacatcaat
     2461 gccgcagccg caggattggt attggtgatg atgactctga cgatgaacgc gcttgctatc
     2521 actttgcgtt atcgcatgcg caagaatctc aaatggtagc gaatgctgaa gaaccggaaa
     2581 tgccggttgc tccgtccgct cagccgcgat ataaatcaga ggtacgaaac ctgagctttt
     2641 attacggctc cttttcggct ctcaaaaaca ttaacatgtt gctgcatgaa aggcaggtga
     2701 ccgcattgat tggtccttcc gggtgcggca aatccacttt tctgcgctgc ttcaaccgca
     2761 tgcacgatct ctatccgggc aatcgttatg tgggtgagat catactgtat ccggataacg
     2821 taaacgtact ttcacggaac gtggatccga tcgaggtgcg gatgcgcatc agcatggttt
     2881 ttcaaaaacc caacccattc ccaaaatcca tttacgaaaa tgttgcatat ggcttgcggg
     2941 tgcgaggggt gcggaagcgt gcaatcctgg atgagaaagt agaagaggcc ttgcggggtg
     3001 ctgcgctatg ggatgaggta aaagatcgtt tgaaccagtt ggccttcaat ctctcgggcg
     3061 gacagcagca gcgtctatgt atagctcgcg cgcttgccac cgacccggaa atcctgctgt
     3121 ttgatgagcc cacttctgcg ctcgacccca ttgcgacggc aagtatcgag gagcttatcg
     3181 ctgaccttaa ggataaggtg accatcctca tcgtgaccca taacatgcag caggccgcgc
     3241 gagtatccga ctatacggcc tacatgtacc tgggtgaggt catcgagttc gacgtcacgg
     3301 acacgatttt catcaaacca aaaaacaagc aaaccgaaga ttacattact ggccggttcg
     3361 gttaggagtt acgatcctcc gtcgaattag ccccgcaact agccccgctc tggcttgtcg
     3421 gaagcatttg gcacgttgcc cctgctcgac gacacgaggt tccatctggt tcctcgccgg
     3481 gttcacggca atccacgcca tcaaccatcg tgctcgccga ggctttcgac acgtcgagct
     3541 ctggctcgcg attgaccgcg tattcattca cgggtaacat tcgcagcttt ccaagttaag
     3601 aagaggggcg gcagctctca tggctatgcg ttcgaagctg gttgcaggca attggaaaat
     3661 gcatggcgaa acggtgcaga atcaggcttt gctgagtgca attcttgata gcgcaagaga
     3721 tctcgatggc atggaaacgg cggtttgtgt gccatatcct tatctgcagc aggttcagac
     3781 agcattgcaa gcgagcgata tttcctgggg cgcgcagaat gtgagccagt atgacaaggg
     3841 agcgtataca ggagaggtat ctgcgggaat gctgatggat tttggctgcc gttatgtcat
     3901 cgtggggcat tctgagcggc gcgccttgta cggcgaggac agccgcacgg ttgcgcttaa
     3961 gttcaaggcc gcccaggcgg ctggactgac tcccattcta tgcgtgggtg aaacactgga
     4021 tcaacgcgag gctggcatta cggagcgggt ggtggcggag caactggatg cggtgagcga
     4081 gttggcaggc gtcgaggcct tggcgaagtc tgtactcgcg tatgagcccg tgtgggcgat
     4141 cggaacaggc aagaccgcaa cgcccggcca ggctcaggac gtgcacacgt tcatccggaa
     4201 caggattgcc gctcgcaatc ctgaagtggg cggcagcttg aaaatccttt atggcggcag
     4261 cgtcaaggct ggcaatgcag gtgaactatt cgcaatgccg gatatcgatg gcggtctggt
     4321 aggtggagca tcccttgtcg cggatgaatt ccttgcaatc tgccgcgccg cgaaatagct
     4381 tttattggag ttgacgtgga aacaatcgtc tggatattac acatcttatc ggctgtcagc
     4441 gttattgtcc tggttctgct tcagcatggg aagggagcgg atatgggagc agcgtttggt
     4501 agcggttcgt ccggcagcct gttcggagca agcggctcgg cgaatttcct gagcagaaca
     4561 accggtgtcc tcgctggcgt ttttttcatt accagtctgg gccttactta tctttccagc
     4621 cataaaatcg aagggggtgg agtgatggat aaagaggttc ccatccaggc cccgaaggca
     4681 gcggagcagg ctcccgaaat accttctgac ggaggtgcgt cgaaggccac ggagataccc
     4741 aagtaattta aatgatgagt taattgaaca attgattaaa gccgcgttct aactaatcag
     4801 cttcagactg ggactcaact aaccgccgac gtggtgaaat tggtagacac gccgtcttga
     4861 gggggcggtg gcgaaagctg tgcgagttcg agtctcgccg tcggcaccag ttcaacagat
     4921 agggctgaga acctgagcat cgcagatcaa tgtaatgccc agattctagt cctttatttt
     4981 tgattgcgag atgctagaaa attatttccc tatcctgttg tttatttttg ttgggcttgc
     5041 cgtcggggtc gtgccgatgt tattagggcg gatgttcggg cccaatcgtc cggatagcga
     5101 gaagctctct ccctacgaat gcggatttga ggcattcgaa gacgcacgca tgaaatttga
     5161 cgtgcgctat tatttggtcg ccatcctgtt catccttttc gatctggaaa tcgccttcct
     5221 gttcccttgg gcggtggtat tgaacgagat cggcttgttc ggtttcattg caatggtggt
     5281 ctttcttggc atcctcatcg ttggtttcat ctacgaatgg accaagggtg ccctcgagtg
     5341 ggattaaagc atgagtgcta acggcggagt agagaaaggt ttcatcacga ccagcctgga
     5401 taacgtcatc aactggggac ggaccggttc catgtggcct atgacctttg gtctcgcatg
     5461 ctgtgcagtg gaaatgatgc aggctggcgc ctcacgctac gacctcgacc gtttcggcgt
     5521 cgtgtttcgt cccagccccc gccagtccga cgtaatgatc gtggcgggca cattatgcaa
     5581 taaaatggcg cccgccctac gcaaggtata cgaccagatg gcggagccgc gctgggtaat
     5641 ttccatgggc acatgcgcga atggtggagg ctactaccat tattcctact ctgtcgtccg
     5701 tggctgcgat cgtatcgttc ccgtcgatat ctacgttccg ggctgccctc ccactgctga
     5761 agctctgctc tacggcatca tccagctgca gaacaagatt caccgcacaa atactattgc
     5821 acgttaaggc aaaatgtcgt cctcccgact ggaagcgctc tccttttgtc ttcagaatgt
     5881 gctgcctgaa cgcctggctg gcattcgtga acggctgggc gagcttacaa tcacactgcg
     5941 tccgtccgat ctgctcggcg tcatgcagac tctgcgcgac cactccgacc tcgcgtttga
     6001 ggttttgatt gatttatgcg gggtcgatta ttcgacctat ggcgccgttt cggttttgga
     6061 caatgagcgc aaaggtcagc gttttgcggt tgtttatcac ctgctttcca taaagcataa
     6121 caccagactg agagtcagga cttttgctga agatgatgaa ttgcccgtgg tggactccgt
     6181 aattgacata tggcccgtgg cgaactggtt tgagcgggag gcattcgatc tttttggcat
     6241 tgttttcaag ggccatccgg acttgcggcg aatccttacc gactatggat tcattggcaa
     6301 ccccttccgc aaggatttcc ctctcagcgg aaacgttgag atgcgctatg acccggatca
     6361 ggggcgagtc atttaccagc ccgtcacgat agaaccccgc cagatcatcc agagagtaat
     6421 ccgggaagaa cattacggag acagcgaatg atggcggacg ctgcggcaga tcgtggacct
     6481 gctccgtgcc ttgttcaaat gttttcaagc ttatagatca tggctgaaat acgaaactac
     6541 accatgaact tcgggcccca gcatccggct gcgcacgggg tgcttcgcct ggtgctggaa
     6601 cttgacggcg aagtgatcca gcgcgccgac cctcatatcg gcttgttgca tcgcgcgacg
     6661 gaaaagctcg ccgagagcaa aagctttatc cagtcggttc cttacatgga caggctcgat
     6721 tacgtgtcca tgatgtgcaa tgagcatgct tacgtcatgg ccatcgaaaa gctgttgcag
     6781 atcgaggttc ctttgcgggc gcaatacgtt cgcgtgatgt tcgacgaaat cacccgaata
     6841 ctcaatcacc ttttgtggtt gggcgcccac gcgctggacg tcggggcaat gaccgttttt
     6901 ctctatgcgt tccgggaccg cgaagacctg atggattgct acgaagcggt atcgggggca
     6961 agaatgcatg ctgcctatta tcgccctggc ggcgtttacc gcgatcttcc cgattcgatg
     7021 ccgcagtatc agccctcgag aattcgtgat gagaaaagca ccgcgctgcg caacgaaaat
     7081 cgtcaaggtt cgctgctgga tttcatagaa gattttacca accggtttcc aggctatgtc
     7141 gatgagtatg aaaccctgct gaccgacaat cgtatctgga aacagcgtct cgtagggatt
     7201 ggggtggtgt cgccggaacg tgccaaggct ctgggtttta ccggtcccat gctgcgcggc
     7261 tccggcgtgg agtgggatct gcggaagaaa cagccttacg aagtatatga ccgcatggat
     7321 tttgatatac cggtcggggt gaatggagat tgctacgacc gctatctggt aagaatcgag
     7381 gaattccgcc agtcaaatcg tattatcaag cagtgcgtgg attggctgcg caagaaccca
     7441 ggcccggtga tcacggataa tcacaaagtg gcgcctcccc ctcggctatc aatgaaaaaa
     7501 aatatggagg agcttattca ccacttcaag ctctttacag aaggcattca tgtgcctgag
     7561 ggcgaggcct acgcagggat cgagcatcca aagggcgaat tcggcattta tcttatctcc
     7621 gatggggcca acatgcctta tcggttgaaa atcagggcgc cgggctttgc ccatttggca
     7681 gcgctggatg aaatgtcgcg tggccatatg atcgccgatg tcgtggcgat aattggcacg
     7741 caggatatcg tattcggtga aatcgacaga tgacatctca ctacattttc agcagaaccg
     7801 taatatcgag atgttaagcg ccgagtcctt acagaaaatc gaccgggaaa tggctaaata
     7861 tcccgccgac caaaagcaat cggcggtgat gtcagcgctt gccattgcac aggatgagaa
     7921 agggtggctg gcgaccgaaa ccatggattt tgtggcccgt tacctcggca tgcctccgat
     7981 agcggtgtac gaagtggcga ctttttacaa tatgtacaac ctccagccgc tcggtaaata
     8041 caagattacc gtgtgcacca acctcccgtg cgccctgtcc ggcgctaatg gggcagtggc
     8101 gcgcatacgg cagcggctgg gaatcggctt caacgagacg accaccgaca acaggtttac
     8161 gctgaaagag ggcgagtgca tgggcgcttg cggcgacgcg cccgtgctcc tggtcaacaa
     8221 caagcgcatg tgcagcttca tgtccgatgc gaagatcgat gaattactgg acgaactgag
     8281 caaatgagcc aaccgacgca aatactgctt gccgggctcg atccatcgga tcccgacaac
     8341 tggcggctca aaagctacga gcagcgcgaa ggctatgccg ctctgaaaaa gattctcaat
     8401 gagaaaattc cgccggaaaa aatcattgag gaagtcaaga aatccgcact gcgcggacgg
     8461 ggtggcgccg gttttccgac cgggctgaag tggagtttca tgcccaagca atatgagggt
     8521 gaaaaatacc tggtatgcaa ctctgacgag ggcgaaccgg gaaccttcaa agatcgcgat
     8581 atcatgcgcc acaacccgca tatcctgatt gaaggcatgc ttatcggcgc ttacgcgatg
     8641 ggcatccgcg ccggctacaa ctatatccac ggcgaaatct gggacgtcta cgaacgcatg
     8701 gaagaggcgg ttgacgaggc ccgcgctgcg ggctatctcg gaaacaatat attgggttcg
     8761 gattttagct ttcagctaca caatcaccat ggctatggtg catatatctg cggcgaggaa
     8821 acggcactgc tcgaatccct cgagggaaag aaggggcagc ctcgtttcaa accccctttt
     8881 cccgcaagct tcgggctcta tggcaagccg acgaccatta acaataccga aacattcgcc
     8941 tcggtgcctt ggatcattcg caatggcggg gagaaatttt tgcagttggg caagcccaat
     9001 aatggaggca caaagctttt ttcggtatct ggacacgtca acaagccggg aaattatgag
     9061 atcccgctgg gtacgccgtt tgcgacactg ctcgaaatgg caggaggcat gcgtggcgga
     9121 cgaaagctga aaggttgcat tcccggcggc tcatccatgc cggtactgcc aggtgatgtc
     9181 atgatgcaga cggacatgga ctacgactcc atcgccaaag ccggttctat gctggggtcc
     9241 ggtgccgtca tcatcatgga cgatacgacc tgcatggtga gggcactgga gaggctttcg
     9301 tacttttatt tcgaggagtc ctgcggtcaa tgcaccccat gccgcgaagg cacaggctgg
     9361 ctctatcggg tggtgcaccg aatcgagacg ggaaaggggc gcatggaaga tttggatctg
     9421 ctcaacaatg tcgctgacaa catacagggg cgtaccatct gcgcgctcgg agatgctgcg
     9481 gcaatgccgg tacgcgcaat gatcaatcat tttcgcgatg agttcgcata ccacatcgag
     9541 cacaagaaat gtctggtgta agagtgccgc tacctgatct gctcgttagc acgagagtgt
     9601 atagacaatg attaatttcg aaatagacgg caaacccgtt tccgttccca agaacgccac
     9661 cgtcatggac ggggccaatc agctggggat attcatcccg catttctgct atca
//
LOCUS       DASSHJ010000038         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_410789, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000038 DASSHJ010000000
VERSION     DASSHJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410789"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFI43_01825"
     CDS             complement(<1..1135)
                     /locus_tag="VFI43_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7060897.1"
                     /translation="MPFKLSLPSPGAIARYEPLNGSGDAFVLAELARQSRPLAIITGS
                     APAAQRLLEEIPFFAPELRIYLLPDWETLPYDTFSPHQDLISERLATLYQLMNGGCDV
                     LIVAATTALYRMPPREYLAAHTFFLKRGEVLDLNAFRAQMTLAGYDNVTQVLSPGEYS
                     LRGGLIDLFPMGSPLPYRIDLLDNEIETIRTFDVDTQRSVYPAKEIRLLPAREFPLDE
                     EGRTRFRGSFREKFEGDPSKSRIYRDISKGSTPAGIEYYLPLFFENTASLFDYLPQST
                     LLCLHQDIHSTVENFWRDTQSRYQLLRGDSDRPVLKPGELFLTTESFFSSLKPYRRVA
                     IEQGSTSGEATSQGLTRPLPPLQIDRRATNPAERLASFTKNFAE"
     gene            complement(1339..>2559)
                     /locus_tag="VFI43_01830"
     CDS             complement(1339..>2559)
                     /locus_tag="VFI43_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7060898.1"
                     /translation="GVMFYVMLALRAELRTRGLFAHPKKEPNLAHLLDLVALGTVADV
                     VKLDHNNRILVHKGLQRIRKGLGCAGIQALLEIARRDFRSVSAYELGFVLGPRLNAAG
                     RLDDMARGIECLITDDPSEARKIALELDGLNRKRKEIEQDMQTAALTMLESLLSKEPR
                     QQLPAISADRNSLCLFDPSWHQGVIGILAARMKEKFHRPAVIFASGNQGELKGSGRSI
                     PGFHLRDALDLISKRHPGLLLKFGGHAAAAGLTIRASDLETFGNAFEETAQALLTPAD
                     LKRFVATDGDLDEAELNLALAEQLEEEVWGQGFPQPTFCARFIVEDQRVVGDRHLKLK
                     LKKYEAGSFRGDAGRAPRSYKAVLFGRNVPLPALVEVVYRLQINEFNGNSTIELIVDH
                     WFQFDASCVTEQLT"
BASE COUNT          582 a          670 c          717 g          590 t
ORIGIN      
        1 gctcagcaaa attcttggtg aatgaagcaa gcctctctgc ggggttcgtc gcgcggcggt
       61 cgatttgaag tggaggcagc ggtctcgtca aaccctgact cgtggcctcg ccacttgtgg
      121 aaccttgctc gattgccacc cgacggtagg gtttcagcga actgaagaaa gattctgtcg
      181 tgaggaacaa ttcgcccggc ttcaacaccg gccggtcgct atccccccgc aacaactgat
      241 aacgggattg cgtatcccgc cagaagtttt ccacggtgga atgaatgtcc tgatgcaggc
      301 aaagcagcgt gctttgaggc aggtaatcga agagggaggc cgtgttttcg aaaaagagtg
      361 gaaggtaata ctcgatacct gcaggggtgg agcctttgct tatgtctcta tagatgcggc
      421 ttttggatgg atcaccctcg aatttttcac gaaaactgcc cctgaaacgg gtacggccct
      481 cctcatcaag aggaaattcg cgtgctggca ataagcgaat ttccttggcg ggatagacgc
      541 tcctttgcgt atcaacatca aaagtccgaa tagtttcgat ttcgttgtcc agcaggtcga
      601 tgcggtaggg cagggggctt cccattggaa acaaatcgat cagtccgccc cgcaggctgt
      661 attcgcctgg cgaaagcacc tgggttacgt tgtcataacc ggcaagggtc atttgcgccc
      721 gaaaggcatt caggtcaagt acttcgcccc gtttgagaaa aaaggtatgg gcagcaaggt
      781 attcccgcgg tggcatgcgg tataacgccg tggtcgccgc gacaataagc acatcgcagc
      841 cgccattcat gagttgatac agggttgcaa gtctttccga aatcaggtct tggtgagggg
      901 agaaggtgtc ataaggcaat gtctcccagt cgggcaataa atagatcctc aactcaggcg
      961 cgaaaaaggg tatttcctcc agcagccgtt gcgccgcggg agcgctcccc gtgatgatgg
     1021 cgaggggccg tgactgccgg gccagttcgg cgagcacgaa cgcgtccccg gaaccattca
     1081 gaggctcgta ccgtgcgatt gcaccgggag aaggaagaga aagcttgaaa ggcatttttt
     1141 taaacgagat gggcgcctta ctggccatgc tcaggttaag ggttgatggc gcgcacatta
     1201 tatgccactt gttccaaaca gatttcctgg acgagtagcg cggggaacgc ggaggttaag
     1261 agcaaaaaat gggcgttgct gtagaactca gtcgaagatc aatcctgagc cgttttgcag
     1321 ttgatctttg actcagcgtt aggtcaattg ttcagttacg catgacgcat cgaactgaaa
     1381 ccagtgatcc acgatgagtt caatggtcga attgccgttg aattcattga tctgaagacg
     1441 atatacgact tctaccagcg cgggcagggg gacatttcgg ccaaacaata ccgctttata
     1501 cgagcgcggc gctctgcctg cgtctcccct gaaggatcca gcttcgtatt ttttcagctt
     1561 caatttcagg tgccggtcgc cgaccacacg ctggtcctcc acgataaagc gtgcgcaaaa
     1621 ggtcggttgc gggaatcctt gtccccatac ctcttcctcg agctgctctg cgagcgcgag
     1681 attcagttcc gcctcatcca gatcgccatc tgttgcaacg aagcgtttga gatcggctgg
     1741 cgtaagcaat gcctgtgctg tctcttcgaa agcgttccca aaagtttcca gatcggaagc
     1801 ccgtattgtc agcccggcag cggctgcatg ccccccgaat ttcaacagaa ggccgggatg
     1861 gcgcttggat atcaggtcca atgcatcgcg gaggtgaaat ccggggatgg atcgtccgga
     1921 tcctttcaat tcaccctggt ttccgctcgc aaatatgact gccggacgat gaaacttctc
     1981 tttcatacga gctgcaagga tccctatcac cccctgatgc catgagggat cgaacaggca
     2041 taggctgttt ctgtcagcgc taatggcagg caactgttgc cgcggctcct ttgaaagcaa
     2101 actttcaagc atggtcagcg cagcggtctg catatcctgt tcgatttcct tacgcttacg
     2161 gttaagccca tcaagctcca gggcaatttt ccgggcttcg gatgggtcgt cagtaatgag
     2221 acactcgatt cctcgcgcca tgtcgtccag tcggcctgcg gcgttcaatc tggggccgag
     2281 aacgaagccc agttcgtatg ccgatacgct gcgaaaatct ctacgtgcta tttcgagtaa
     2341 ggcctggata cccgcgcaac ccagcccctt gcgtattcgc tggagacctt tgtgtacaag
     2401 aatacggttg ttgtgatcga gcttgacgac gtcggcgacc gtgcccagcg caaccagatc
     2461 gagcagatgt gcgagattag gttctttttt agggtgtgca aacaagcctc gggtccgcag
     2521 ttcggcgcga agggccagca tgacgtaaaa catcacgcc
//
LOCUS       DASSHJ010000039         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_736332, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000039 DASSHJ010000000
VERSION     DASSHJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736332"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFI43_01835"
     CDS             complement(<1..724)
                     /locus_tag="VFI43_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET7060899.1"
                     /translation="MKFKQKGGKNFWAPLMPMAFALGLASCGDGKGPSDSGSGSGSAS
                     SASSSTEKGPTTGRLLDTAVEGVAFTASSGATGTTDKQGTFKYSHGDTVEFKLGSLVL
                     GKPKGAAIITPMELAGESPARLQNILILLQSLDVDGNPDNGISIPASAAAAVTASINL
                     ESDPAAFSTSPELQKAREGGSVAGAAKTPEQAEAHFLSQGVKLLSTNIWAQYDDATAS
                     IIRASTAEGGEYLEGEATPDDSC"
     gene            complement(909..1454)
                     /locus_tag="VFI43_01840"
     CDS             complement(909..1454)
                     /locus_tag="VFI43_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HET7060900.1"
                     /translation="MKKILLALILVAPVTLTGCMGLPKGVAPVGNFDISRYLGTWYEI
                     ARLDHSFERSLSDITATYSLRDDGGIKVVNRGCDVRKGEWRTAKGVAYPVKDPYTGYL
                     KVSFFGPFYGSYVVFDLDKIGYQYSFVTGPDRSYLWLLSRSPRVEDEVLKKFIGEAGE
                     LGFDTGKLIYVEHDSQSCEMK"
     gene            complement(1527..3233)
                     /locus_tag="VFI43_01845"
     CDS             complement(1527..3233)
                     /locus_tag="VFI43_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060901.1"
                     /translation="MNIAKVLFLILAFGLSVALPVQATTLKVLQDIGSPNSVQFLQND
                     WNPADGDTHWQTVAPDSMSVATDPSVLDFNGAIVAGTFVVGDDPWETVSIKSNAGQQF
                     DLRKTQEFQLLARPQYVTANPYITVVLRLTMQDGSIWDQPKVLANNVWQKGAFPVQDG
                     SFTRVEWGPEGIFDLGKVASWEVILVDLPPSSSHRMDFRAMQMKGNYDAPAGTDVAEP
                     FGVDFFNGDENILFRRADWNPSDGDTDWLYHVRSTPIYYVPYGFLRALYRYGGDAWET
                     VSIRKEAQTYFDLSANQDRQIVALVDAGLNPDPNALILSLTMEDGSVWQQGRPVDVVR
                     KTALRHYTHAGDIVLPYRFPLDPNGKGWTMAAWAPPGSFNLNRVKAWELYFNNLEQGS
                     HGVVLSTVDGTTASSLVRADNTLSAGGQAQWLDTSFASDGVVANINSAGGITDNLSIA
                     SKNPVGPLGLGFNLEVTTSYVNASPDALVFKLTASDGKVWQQAFDLPQPGRSVLRIYT
                     SCPSTYPQMVRPDPDDLRCKGILGSFYGINPADISKWEIILNRPAAGQHQVKVNLLST
                     ID"
     gene            complement(3230..3526)
                     /locus_tag="VFI43_01850"
     CDS             complement(3230..3526)
                     /locus_tag="VFI43_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060902.1"
                     /translation="MVVRLFVVERWAKKDERICRPAVLGAFCALSRFCQENGKKSCRS
                     ISSAQPFLLNEKFELELIYAYGLSSRGMKYKYEIWGVSKIEAGNFPISKGGRKI"
     gene            3698..4906
                     /locus_tag="VFI43_01855"
     CDS             3698..4906
                     /locus_tag="VFI43_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7060903.1"
                     /translation="MKRRLCLILWGVVNLFLLCFSAWASEGSGELNEARQVAQYNYVI
                     HILAMLLVGFGFLMVFVRRYGFGATTGTYLVVAVGLPLYMLMRANGIFAHEIAPNTVK
                     ALMYAEFAVASALIAMGAVLGRLRVFQYALLALFLVPSYLLNEWLVLDNGMGLTEGFQ
                     DTAGSIIIHAFGAYFGLALSLALTTAPQRNQPIESDPTSDRFAMLGSMVLWLFWPSFA
                     TAIVPFEEMPQTVVNTVLALCGATLSTYFLSSWFHKGKTSIVDMANAALAGGVAIGST
                     CNIVSPTGAFGIGLLAGGVSVIGYVFIQPALEARLKLVDTCGVHNLHGMPGLLGAVVA
                     IFVVPGIAGAQIIGIVFSVVLALITGTVAGLLIKASGTTRLAYEDSEEFTHVEGPETE
                     HMPELKAGTH"
BASE COUNT         1129 a         1390 c         1308 g         1211 t
ORIGIN      
        1 cgcatgaatc gtccggtgta gcttcccctt ccaagtactc tcctccctcc gcggtggaag
       61 cacggatgat ggaagcggtt gcatcgtcgt attgagccca gatattggtg ctgaggagct
      121 tgacaccctg ggaaagaaag tgagcctcgg cctgctccgg cgtctttgcc gcaccggcaa
      181 cgctcccccc ctctctcgcc ttctgtaatt cgggagacgt tgaaaatgcc gcaggatcac
      241 tctccaggtt gatcgaagca gtaacagccg ctgcagcact cgccgggatc gatatgccgt
      301 tgtcagggtt gccgtccaca tcgagggatt gcaacaggat gagaatattt tgcagccgcg
      361 cagggctttc tcctgccaac tccatgggag ttatgatggc tgcgcccttg ggtttgccaa
      421 gcacgagact tccgagcttg aactcaaccg tatccccatg actgtacttg aaggtcccct
      481 gcttgtccgt ggttccggtt gcccccgatg aggcggtaaa agccaccccc tccactgccg
      541 tatcgagcaa gcggccggtt gtcgggcctt tttctgtcga gctggacgca ctgcttgcac
      601 taccactgcc gctcccagaa tctgacggac ctttgccatc gccgcatgaa gcgagaccga
      661 gagcaaatgc catgggcatc aaaggcgccc agaagttttt cccacccttt tgtttgaatt
      721 tcattccgcg aatccattcc ctgtttaatg acgcgtaatg atatcaaact ttatctcggc
      781 aagacttaaa agcagaggtg gttcgcagag cagctatcaa tcaatcttcg gggagaagat
      841 ttactctgac tttatgctcc cctgcgggcg cctgctcgga agcatttccc acttcaagat
      901 tttggagctc acttcatctc acaggattgg gaatcgtgct cgacgtatat aagcttcccg
      961 gtgtcaaagc cgagttcgcc cgcttccccg ataaactttt tcaaaacctc atcttcgaca
     1021 cgaggactcc ttgagagaag ccacagatag cttctgtccg gcccggtcac aaaggaatac
     1081 tggtaaccga tcttatccag atcgaatacc acgtatgaac catagaaagg accaaaaaaa
     1141 gaaaccttga ggtagcccgt gtaagggtct tttacagggt acgccacgcc ttttgccgtt
     1201 cgccactcgc ctttccggac gtcgcacccc cggttcacga ccttgatgcc gccatcatcg
     1261 cgcaagctat aggttgcagt aatgtcgctc aaactccttt caaacgaatg gtccagccgg
     1321 gcaatttcat accatgttcc caagtagcga gagatatcaa aattccccac aggagcgacg
     1381 cccttcggaa gccccatgca cccggttagc gtgacaggcg caaccaagat taacgcaagt
     1441 aatatttttt tcatcaattg acaacaatca ttctgcaaac cggcttaaca gaagccgaga
     1501 cggaatacct cattcgagcg cccggttcaa tcaatggtcg agagaagatt aaccttgact
     1561 tgatgttgtc ctgcggccgg tctgttcaag atgatttccc atttcgaaat atcagcaggg
     1621 ttgattccat aaaaacttcc cagaatgccc ttgcaacgta ggtcgtccgg gtccggccgc
     1681 accatctggg ggtatgtgga aggacaagac gtataaatcc tcaggaccga tcgtccaggc
     1741 tgcggcagat caaaggcctg ctgccacacc tttccatcgc ttgccgtgag tttgaatacg
     1801 agcgcgtctg gagaggcatt tacatagctg gtggtgactt caaggttaaa gcccaatccc
     1861 aacgggccaa ccggattttt cgaagcaatg gagaggttgt cggttatacc accggctgaa
     1921 ttaatgttcg caacaacccc atccgaagca aagctggtgt cgagccattg cgcctgcccc
     1981 ccggcgctga gtgtattgtc tgctcgcacc agagaagagg cggtcgtgcc atcaacggtg
     2041 ctgagcacca caccgtgaga tccttgctca agattattga aatacagttc ccatgccttg
     2101 acccgattga ggttaaagct accgggaggt gcccacgctg ccattgtcca gccttttccg
     2161 ttaggatcga ggggaaaccg gtagggaaga acaatgtcgc cagcatgagt atagtgtcgc
     2221 aaggcagtct ttctaaccac atccaccggc cttccctgct gccagacgct tccgtcctcc
     2281 atcgtcaggc tcaagatgag agcgttagga tccggattga ggccagcgtc tacaagggcc
     2341 acgatctggc ggtcctgatt ggccgacaaa tcaaagtagg tttgtgcctc ctttcgtatc
     2401 gataccgtct cccacgcgtc gcccccataa cggtagagcg ccctcagaaa tccgtacggc
     2461 acataataga taggggttga acgtacgtga tacagccagt cggtatctcc atcagacggg
     2521 ttccagtcgg ctcggcgaaa caggatgttt tcgtccccgt tgaaaaaatc taccccgaat
     2581 ggctcggcca cgtcggtacc agcaggcgca tcataatttc ctttcatctg catcgcgcga
     2641 aagtccatgc gatggctact gcttggaggc agatcaacga ggataacttc ccatgaggca
     2701 actttgccaa gatcaaatat accttccggc ccccattcga cccgggtaaa tgagccgtcc
     2761 tggacgggaa acgccccttt ttgccagaca ttgtttgcca aaaccttggg ctggtcccag
     2821 atactgccgt cctgcattgt caggcgtagc acgaccgtga tatatgggtt ggccgtgacg
     2881 tactgcgggc gagcgagcag ctgaaattcc tgcgttttcc tcaggtcgaa ttgttgtcca
     2941 gcattgcttt ttatcgagac ggtttcccag gggtcatctc caacaacgaa ggttcccgcc
     3001 acgattgctc cgttaaaatc cagcacagaa gggtccgtag caacagacat gctatctggc
     3061 gcaacggttt gccagtgtgt atcgccgtct gcagggttcc agtcattctg cagaaactgt
     3121 accgaattcg gtgaaccgat gtcctgaagc accttgagcg tggttgcctg cacaggcaaa
     3181 gccacagaca gaccgaatgc cagaatcaaa aagagaacct tcgcgatatt catatttttc
     3241 ttcccccttt tgagatagga aaatttcctg cctctatttt agaaactccc catatttcat
     3301 atttatattt catccccctg ctcgacaacc catatgcata aattaactct agctcgaatt
     3361 tttcattaag caagaaaggc tgagccgaag aaatggatcg acaggatttc ttgccatttt
     3421 cctggcaaaa gcggcttaat gcacaaaaag cgcccaagac agcaggccga catatccgct
     3481 catctttttt ggcccatctt tcaactacga aaagtctgac gaccatccgc ccgttctgac
     3541 acctctgccg cgcctgctgc ttcttccctc tttttcagct ttattgcttc acgaaatcag
     3601 aataacaaat cgcataaatc cccattgtgt tcgaggctat tgaggttcaa actcttgcat
     3661 gggtatgcca aataattgct gaacatgggg ggtcaaaatg aaaagaaggc tctgcttgat
     3721 actgtggggt gtcgtaaacc tgtttctgct gtgttttagt gcctgggcga gtgaaggtag
     3781 tggggaactc aacgaagcca ggcaggtggc ccaatataac tatgtgattc acatcctcgc
     3841 gatgttgctg gtgggtttcg gttttctgat ggtgtttgtc cgaagatacg gctttggggc
     3901 tacgaccggc acgtaccttg tggtggctgt cggtctaccc ctctatatgc tcatgcgagc
     3961 gaatgggata tttgctcatg agattgcgcc gaatacggta aaggcgctga tgtatgcgga
     4021 attcgcggtg gcgtccgcgt tgattgccat gggcgcagtg ctcgggcgac tgcgcgtttt
     4081 ccaatatgca ctgctcgcac tattcctcgt gccttcgtac ctgctgaacg aatggctggt
     4141 gctcgacaac gggatgggtc tgacggaagg atttcaggat actgccggat caatcattat
     4201 ccatgccttt ggcgcctatt tcggattggc gctgtccctt gcgctgacca ctgcgcctca
     4261 gcgcaaccaa ccgatcgagt cggaccccac ctccgaccgg ttcgccatgc tgggttcgat
     4321 ggtactctgg cttttctggc cgagcttcgc cactgccatc gttccgtttg aggaaatgcc
     4381 ccagaccgtg gtcaacacgg tgcttgcgct gtgcggcgcc acgctgagca cgtactttct
     4441 aagctcgtgg tttcataagg gcaagacatc gatcgttgac atggccaatg ccgcgctggc
     4501 aggcggtgtg gcgatcgggt cgacctgcaa catcgtgtcc cccaccggag cattcggaat
     4561 cggcttgctc gcagggggtg tgagcgtcat cggttatgta tttattcagc ccgcgctcga
     4621 agcgcggttg aagctggtgg atacttgcgg cgtccacaat ctgcacggga tgccgggatt
     4681 gctgggtgcg gtagtcgcta tcttcgtcgt gcctggcata gccggcgctc aaatcatcgg
     4741 cattgtgttc agcgtggtgc ttgcacttat cacaggtacc gtcgcaggcc tgctgataaa
     4801 agcctccggc acgactcgtc tggcctacga ggacagcgag gaattcacgc atgtcgaagg
     4861 cccggagaca gagcatatgc cggagttgaa ggccggcacg cattgacgag tctctgagcc
     4921 agactgggga gtgacggcct ttatatgtga gattggatgc gcgaggagcg gatgggggcg
     4981 acgccaggag caacgtagta tcgcccttat cgaggccgcc gctttctgca cgcttgcc
//
LOCUS       DASSHJ010000040        11535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1500455,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000040 DASSHJ010000000
VERSION     DASSHJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11535
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500455"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..621
                     /gene="smbP"
                     /locus_tag="VFI43_01860"
     CDS             199..621
                     /gene="smbP"
                     /locus_tag="VFI43_01860"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HET7060904.1"
                     /translation="MISIAKTSAEGEMGFPRTRKLMLFAICCMCSHISAHAEDHGKKA
                     REHLQEAIESGKKGNAADVLFHTEEARRELIEDNKEHPYTQLQKPIYGEHEKAEHDKE
                     AFEDIDEAIDEAEDGDAKDAAEAAARASAHLKEKEEAK"
     gene            complement(712..3312)
                     /gene="ligD"
                     /locus_tag="VFI43_01865"
     CDS             complement(712..3312)
                     /gene="ligD"
                     /locus_tag="VFI43_01865"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET7060905.1"
                     /translation="MTTRTSRTPDSSRADPSEGDPLKAYRDKRDFSITSEPSGVEKAP
                     RAKKATPLAFVIQKHWATRLHYDLRLELEGTMKSWAVPKGPSFDPNDKRMAVHVEDHP
                     ISYNTFEGEIPAGEYGAGKVIVWDKGIWVPLENAHTGYREGKLKFELHGYKLKGHWTL
                     VRMRNDREQKQDAWLLIKEKDMYARPSSEYSVVDEMPDSVSPLKALTLGESAELQGKD
                     ANHAVDEEIQATKNKVALPSGAIKAKLPKLLEPELATLIDSPPGDPEEWVFEIKFDGY
                     RLVTRIDEGGIHVFTRNGHDWTDKLPDLVKSIQKLELKPCWLDGEIVVMDENNLPDFQ
                     ALQNAFDNERTQNISYFLFDLPFYDGYDLRNCSLLERKQWLKAVLKKNTQENIHVSEF
                     FDAPPGEVLNSACRIGLEGIIGKRKNSTYVSRRTTGWVKLKCGRRQEFVIGGYTDPKG
                     TRFGIGSLLLGYYDENGALQYAGNVGSGFNEKTLRELREKFDDLATEKCPFVNERGIE
                     KKAHWVKPVLVAEVSFSEWTRGNHVRQAVFRGLRTDKKPDSIVREKATHVDPPKTAIR
                     TSIPETLKITNPDRVVDPLTGFRKIDLIRYYALVAPLMMEHLKGRPVSLVRAPEGIGG
                     ELFFQKHWEKGNTEGINQLDPALDPDHESLTEISNAEGLLTAAQMNVVEFHTWNAKKD
                     LIGKPDRMTFDLDPGEGVEWPAVQESAELVSIFLNQLGLKSFLKTTGGKGLHVVVPIR
                     RTHGWDTVKDFSQAIVQHLANTIPGRFVAKSGPRNRVGKIFIDYLRNGFGATTVSAWS
                     VRARPGLGVSVPITWQELGSVSSSSHWTALNIHQRLDKGNEPWADYDHVKQSITPAMK
                     TLGFEADKKA"
     gene            complement(3319..4212)
                     /locus_tag="VFI43_01870"
     CDS             complement(3319..4212)
                     /locus_tag="VFI43_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7060906.1"
                     /translation="MVASTSRALWKGAISFGLVHIPVALHTATSEQGVNFDWLDKRTM
                     DPVGYKRINKKTGKEVSKEDIVKGIEYEDGQYVILSPEEIAAAYPKTTQTIGIETFVG
                     ADDIPFIYLERPYYVVPINKGEKVYALLREALSRTKRVGIARVVIQTKQHLAALMPSG
                     PLLILNLLRWSDEIRSTKSLSVPPEGTDALGISDRELKMAEQLVTEMSSTWNPEDYRD
                     SFRDEIMKLVEEKAKKGEIQAVSRPEEAEGVRAGAQILDLTELLQRSLKGGKKEEPEK
                     DTGAKAERKSPAKASHKRRAG"
     gene            4704..4928
                     /locus_tag="VFI43_01875"
     CDS             4704..4928
                     /locus_tag="VFI43_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060907.1"
                     /translation="MDISLLQWPAMAVNVLSVWLLTSQARRTRHVGFSLSLISNVLWI
                     IWGLHIQAFAVLVLQVALAALNIRGVRKTD"
     gene            5325..7076
                     /locus_tag="VFI43_01880"
     CDS             5325..7076
                     /locus_tag="VFI43_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7060908.1"
                     /translation="MRILLTYCALFPRRTTYVLIALLAAGIAEGLSLTALLPLLSIAV
                     GDPGNSDLGRRILELLRAGGIEPTIGVMLLLIVTGMVLKSLILLLANRQVGFTVAHVA
                     TALRLELIDALLGSRWQYYLRQPMGSLANSVATEAYRAANGFEHGVTVLALVVQVIVY
                     GTVAMFISWEATLTSFMIGIIILHLLHRLIRSTRKAGAKQTQLLRHLLTYLSDVLGSV
                     KSLKAMGRDKVADAILRDQTKQLEKATRREVMAREALTALQEPILAIIMALGLYIALV
                     MWELSLPSVMVIAFLLTRILGLLNKTQRKYQNLSAQESAYWALRAAIEEARTAAERTT
                     GSRQPSLREEISLRRVNFAYGPKIIFQELDLEIPVKSLTTIAGASGIGKSTLLDLLCG
                     LAEPESGEILIDGVPLGQINPREWRRMIGYVSQENILLHDTILNNILVGAPDLKEADA
                     ERALREAGAWDFVCALPEGVRTIVGERGGLLSGGQRQRIAIARALAHQPVLLLLDEPT
                     SALDPETERKVCETLQKLARDITVVAVSHQPVLINAADNIFTLSKGKAERLQRTTKVE
                     EDFSAMPASRISAVASH"
     gene            complement(7136..8512)
                     /gene="cls"
                     /locus_tag="VFI43_01885"
     CDS             complement(7136..8512)
                     /gene="cls"
                     /locus_tag="VFI43_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET7060909.1"
                     /translation="MTILKYPALLCLLLTFGCSATQLTQRITLRHPTSSIPPVLVEGA
                     HGPLSVQQTREILARLKQSAPETSIFTRHLALEEEIAGSPLMTGNKITLLIDGPATYK
                     SMYAAIQGAKNHINLETYDIEADEVGRKFAGALMAKQKSGVQVNLIYDAFGSSNTPDS
                     FFKQLADTGVRILEFNPINPLMLTREWEVGRDHRKLLVVDGRVAFVGGVNISSVYSSG
                     SFTSKPHGKNTQPWRDTHIRIEGPVVAEFQKFFLTTWVTQKGGPLGTEVFFPPPIVKG
                     TEVVRAISTRPIDHYSPIYVTLVSAINSAETYAYITNAYFVPDVDLLEALKNAARRNV
                     DVRLLLPGKTDSNIVFYASRSYYDELLSAGVKIYERQDALLHAKTAVIDGVWSTIGST
                     NLDWLSSEHNQEINAVILGQEFGEQMKSLFGKDMDASTLVTLEEWRKRPIELRLKEFA
                     ARLLSGWI"
     gene            complement(8726..9328)
                     /locus_tag="VFI43_01890"
     CDS             complement(8726..9328)
                     /locus_tag="VFI43_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7060910.1"
                     /translation="MKKIRFQTITVLLGMILTYSLGLVIEDSGAGQPSQTGKKQQGAT
                     SMKIESSSFPNNGPIPARLTCDGHDISPGLFWADVPQGTKSLVLIIDDPDAPDPAAPQ
                     RTWVHWVLYNIPPDAPGLPEGIERRALPPGILEGVNDWNRTGYGGPCPPIGNHRYFHK
                     LYALDVVLPDLNHPTKPELEKAMQGHVIGHSELIGRYQKQ"
     gene            complement(9357..9869)
                     /locus_tag="VFI43_01895"
     CDS             complement(9357..9869)
                     /locus_tag="VFI43_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7060911.1"
                     /translation="MLDTLKQAGKHISRSLGRTWDNISEGWRELVHHSSDALTHFSRR
                     EDQGKEKQQAANAFPVFRNWSLLAGDVEETDKEIVVRMEVPGMEKKDCEITIDGNILY
                     VRGEKRFERVTQDSTYHIMERAYGSFERAIPLPRNVDTEKAEASYTNGVLTVRLPKSD
                     TGAGRMIPVS"
     gene            complement(9909..10358)
                     /locus_tag="VFI43_01900"
     CDS             complement(9909..10358)
                     /locus_tag="VFI43_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7060912.1"
                     /translation="MPIGEICSREVIVVRRSDSVLEAAKLMRRHHVGDVVVVDERDGI
                     RVPVGLVTDRDVVVEIIATEIDPGVITVEDIMEPDLACARDSTGIFETIQYMQDRKVR
                     RLPIVDERGALLGILSLDDLLELLSEELLAMAKLVRYQQQTETRFRQ"
     gene            complement(10464..10970)
                     /locus_tag="VFI43_01905"
     CDS             complement(10464..10970)
                     /locus_tag="VFI43_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HET7060913.1"
                     /translation="MGTHTTPPPGFHRIKRLDEMLQERVHDPYRARSKPKEPSTCPRC
                     GAVFHDGRWEWREAPEGAHQEMCPACHRINDQYPAGFLTLKGQFFATHREEILHLARN
                     VEKRERTEHPLNRIIAIEEEDDGMLLTTTDIHLVRGIGEAIHDAYQGDLDFHYNPDEY
                     LLRVAWER"
     gene            complement(11228..>11535)
                     /locus_tag="VFI43_01910"
     CDS             complement(11228..>11535)
                     /locus_tag="VFI43_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7060914.1"
                     /translation="VTEDDNAYHVRAEIPGVKKEDVKVQVEGNRVSISAENKQENEEK
                     EGERVLYRECHQGSSYRTFTLGSEVDASKAEAKYDNGTLELTLPKKEGKTAKRIEVK"
BASE COUNT         2464 a         3222 c         2987 g         2862 t
ORIGIN      
        1 gccgcctaca gcatgttaat gatgggatgg acggaaggcc aggcttactc tgagatggaa
       61 ctgtcaaaaa ttctgacatc cgtgggattt caggatatcc agatccgacc tacattcggg
      121 tattacagca ttgtcagcgg agtcaaggcc tgagcaggcg tatattgcac agtttgagca
      181 tgggtcgggg gtaatgttat gattagcatt gccaagactt cggcggaggg tgaaatggga
      241 ttcccacgta cacgcaagct gatgttgttc gccatttgtt gcatgtgcag ccatatttct
      301 gcccatgctg aagatcacgg aaagaaggcc cgggagcatt tgcaggaggc tattgaaagc
      361 gggaaaaaag ggaacgctgc ggacgttctg ttccataccg aagaggcgcg ccgggaattg
      421 atcgaggata acaaggaaca tccgtatacc cagctgcaaa agcccatata cggggaacat
      481 gagaaggcgg agcatgacaa ggaggccttt gaagatatag acgaggccat tgacgaagcc
      541 gaggatggtg acgccaagga tgctgcggag gccgccgcaa gagcttccgc tcacctcaag
      601 gaaaaagaag aagccaaata gctcgatgtt ctctttgcgc tgccaggttg aatcgctcgc
      661 cgcgttctgg cgattcacgg ttcaggttct gcgggcaagc cggttgacgc ttcaggcttt
      721 cttgtcagcc tcgaacccca gcgttttcat tgcgggggtg atggattgct tcacatgatc
      781 gtaatccgcc cacggctcgt ttcccttatc caggcgttgg tggatattca gggccgtcca
      841 atggctgctg ctggagactg acccgagctc ttgccatgta attggcactg aaacccccaa
      901 cccggggcgc gcccgtaccg accatgctga gacggtggta gccccaaagc cattgcgaag
      961 atagtcaata aaaatttttc ccacgcgatt ccggggaccg cttttcgcta caaaccgtcc
     1021 tgggatcgtg ttggcgagat gctgaacgat ggcttgagag aagtccttta ccgtgtccca
     1081 accgtgcgta cgcctgatgg gaacgacgac gtgcagtccc ttccctccgg tggttttaag
     1141 gaaggatttc aatccgagct ggttcagaaa tatcgaaacg agttctgccg attcctgcac
     1201 cgccggccac tccacgcctt cacccggatc aagatcgaat gtcatcctgt ccggcttgcc
     1261 aatcaagtcc tttttcgcgt tccaggtgtg aaactcgacc acattcatct gcgctgcggt
     1321 cagaaggcct tccgcatttg aaatttcggt gagggactcg tgatcaggat cgagtgcagg
     1381 gtcaagctgg ttgattccct ccgtgtttcc cttttcccag tgcttctgga aaaacagctc
     1441 gcctcctatt ccctctggcg cccgtacaag ggatacaggc ctgcctttca agtgctccat
     1501 catcaagggg gcgaccaggg cgtaataccg gatgaggtct attttcctga atcccgtcaa
     1561 tggatcgaca acccggtcag ggttggttat ctttaacgtt tcaggaatcg aagtcctgat
     1621 tgcagttttt ggcggatcga catgcgtcgc cttctcccgg acgatggaat ccggcttctt
     1681 atctgtccgc agccctcgga acacggcttg ccggacgtgg ttgccccggg tccattcgct
     1741 gaaggatacc tccgctacaa gcactggttt gacccagtgc gctttttttt caatcccacg
     1801 ctcgttcacg aaggggcatt tctcagttgc aagatcgtca aatttctccc tcagctcccg
     1861 caatgtcttt tcgttgaaac ccgatccgac atttcctgca tactgcaggg ctccgttttc
     1921 atcataataa ccaagcaaaa gagagccgat gccgaatcgc gtccctttgg gatctgtata
     1981 gcccccgata acgaattcct gccgccggcc gcatttgagc ttcacccatc cggtagtgcg
     2041 gcgggaaaca tacgtggagt tcttgcgttt gccaatgatg ccttccagcc cgatccggca
     2101 cgcagagttg agcacttccc cggggggcgc atcgaaaaat tcactgacgt gtatattttc
     2161 ctgggtattt ttcttgagca ctgctttcaa ccactgtttt cgctcaagca atgagcagtt
     2221 ccgcaggtcg tatccgtcgt aaaaaggtag atcaaagaga aaataagaaa tattctgggt
     2281 tctttcgtta tcgaacgcgt tctgcaatgc ctgaaaatcc ggaaggttgt tttcatccat
     2341 taccacgatt tcgccatcca gccaacacgg cttcagctcg agcttttgta tggacttgac
     2401 cagatcaggc aatttgtccg tccagtcatg gccgttgcgg gtaaaaacgt gaattccacc
     2461 ttcatctatc ctcgtcacca accggtagcc gtcgaatttg atttcaaata cccactcttc
     2521 aggatccccg gggggcgaat cgatcagggt agcgagctca ggttcgagta attttggcaa
     2581 cttcgccttt atcgcgcctg atggcaacgc taccttgttt ttcgttgcct ggatctcttc
     2641 gtccacggca tggttcgcat ctttgccctg cagctcggcg gactcaccca aagtaagcgc
     2701 tttcaggggg gaaacgctgt ctggcatctc gtccaccacg ctgtattcgc tggatgggcg
     2761 cgcgtacatg tccttttcct tgataagcag ccaggcgtcc tgcttctgct cgcggtcatt
     2821 tctcatgcgc acgagcgtcc agtgcccttt aagcttgtat ccgtgcaatt cgaatttgag
     2881 cttaccctcc cggtaaccgg tatgggcgtt ctcaagcggc acccatattc ccttatccca
     2941 gacaatgacc ttgccggctc catactctcc agccggaatc tcgccttcga aggtattgta
     3001 ggagatcgga tgatcctcca cgtgaacggc cattcgtttg tcgttgggat cgaagctcgg
     3061 gcctttggga accgcccagc ttttcatggt tccctcaagc tccagcctca ggtcgtaatg
     3121 caaacgcgtg gcccagtgct tctgtatgac gaaggccagt ggagtggcct tcttcgccct
     3181 gggtgccttt tcgacgcccg agggttcgga cgttatcgaa aaatcgcgct tatcccgata
     3241 cgccttgaga ggatcccctt ctgaaggatc agccctggag gaatcaggtg ttcgggaagt
     3301 gcgtgttgtc attccaaact accccgctct ccgcttgtgg cttgccttgg cgggtgactt
     3361 gcgctcagcc ttggcgcccg tgtccttttc gggctcttct ttcttgccac ccttgaggct
     3421 gcgctgaagc agttcggtga gatcaagaat ttgagcaccg gcacgcaccc cttctgcctc
     3481 ttccggccgt gacaccgcct ggatttcgcc ttttttggcc ttctcttcta cgagcttcat
     3541 gatctcgtcc ctgaatgaat cgcggtagtc ttcggggttc catgttgaac tcatctcggt
     3601 aaccaactgc tcagccatct tcaactctct gtcgctgatg cccaatgcgt cggtcccttc
     3661 cgggggaacg ctcaaactct tcgtgcttct tatttcatcg ctccaacgca acagattcag
     3721 tatgagcagg ggacccgagg gcatgagcgc ggccaggtgc tgctttgtct ggatgactac
     3781 ccttgcgatc ccgacacgct tcgtcctcga aagcgcttcc cgaagaagcg catacacttt
     3841 ttcacctttg ttgatcggaa cgacataata gggacgctca agatagatga agggaatatc
     3901 atcagcacca acgaaagttt caatacctat ggtttgcgtg gttttgggat atgccgcagc
     3961 gatttcctcc gggctcagaa tcacgtattg cccatcttcg tattcgattc ccttgacgat
     4021 atcttccttt gacacctctt tccccgtttt tttattgatc cgcttgtaac ccactggatc
     4081 catcgtgcgc ttgtccagcc agtcaaagtt caccccttgc tcggacgtgg cggtatgaag
     4141 cgcgacaggg atgtgaacga ggccgaagct aatggctcct ttccataaag cccggctggt
     4201 ggaagcaacc ataagtctcc tctattaaag ggaaaatgag gcaaaacggc aattccaata
     4261 acattttcca tcacatcgat cacgaggtat gtacgattac ccacagaaaa caccaagctg
     4321 gactgtcaga ctgctgacgg caataaaggg acaaggactt cgtcgagttc agaagaaggg
     4381 cgttgcgtga aagggggcga aaaaacgatc agttattggc cgcgctaatc ctctgtcagc
     4441 gacccaggcc aaggcttcta tggctgatct tgaaaaaaat gcgccgatac caccgcgcct
     4501 gggcgggatt tctgttcgtc cgaaacctcc aggtgcttga aatcccctgg accctgcatg
     4561 tgaaccaggc cagaagcaat tctgacgcgc taatatggta cgagcaggcg gggcagtgcc
     4621 gattacgtct ggcccaagct tacagactcc acttcgaaaa aaacaagcgt gatttcttcg
     4681 catcatgcat ttaaaggctg aacatggata tatcattgct acagtggccc gccatggccg
     4741 tcaacgtact ttcggtctgg ctgctgactt cgcaagccag gcgcacgcgc cacgttggtt
     4801 tttcactttc tctcatcagt aatgttctat ggattatctg gggcttgcac atccaggcgt
     4861 ttgcggtact cgtcctccag gttgcgttgg cagcgcttaa tattcggggc gtaagaaaga
     4921 cggattagat aaataccgga tcccaagcat aatcgtcacg tttatcgacg aaatgcgttc
     4981 gtgattccgc ttattcacgt gcaaggctgt gctttgcgtg tcaataagtg ataaaatgat
     5041 attgaatgtc ggaaccgacg gcatttcagc ggagcgaatg cttgaggaag cctccataca
     5101 ggccggggtc gaagacgttc cccggcttcc ttgagcgccg ccgggcaaat ggaagcattt
     5161 aaatatctac ggcctgctcc tcttcataat cggcggggaa accttcgcga tatctccgtt
     5221 tccccacttc tgccacttcc ttttcccgga cagcccttgt tcaagtgcct cagatttaag
     5281 ccgctcggca cgcccataat tacggactac acgcaaacct ggccatgcgt atcctcctaa
     5341 cctactgcgc gctgttcccg cggcgtacga catacgttct aattgcgcta ctagccgccg
     5401 ggattgccga aggtctcagc ttgacggcct tgctcccgct gctctcgatc gccgttggcg
     5461 accctggcaa ttccgacctt ggacgccgaa tcctcgaatt gctgagagcc ggaggcatag
     5521 aaccgacgat cggcgtcatg ttgctgctca tcgtaacggg catggtgctg aaaagcctga
     5581 ttctcctgct ggccaacagg caggtaggct ttactgtcgc gcacgtcgcg accgccctgc
     5641 gcctggagct catcgatgcg ttgctgggaa gccgctggca gtactatttg cgccagccca
     5701 tgggctcgtt ggcgaactcg gtggcgacgg aagcgtaccg ggcggcgaat ggcttcgagc
     5761 atggcgtgac cgtacttgcg ctggtcgtgc aggtcatcgt gtacggcacg gtagcaatgt
     5821 tcatctcgtg ggaggcgacc ctgacctcct tcatgatcgg aatcatcatt ctgcacctcc
     5881 tgcaccggtt aatacgctct actcgcaagg ccggcgcgaa gcagacccag cttttgcgcc
     5941 accttctgac atatctttca gatgtgctcg gctcggtgaa atcactaaaa gccatgggac
     6001 gcgacaaggt tgccgacgct atcctgcgcg atcaaacgaa gcagctcgag aaggcgacga
     6061 gacgagaagt catggcccgc gaagcactta ccgcattgca ggagcccata ctggccatca
     6121 tcatggccct gggcttgtat atagccttgg tgatgtggga gctttccttg ccttcggtaa
     6181 tggtcattgc cttcctgctt acccgtattc tgggcctgct gaataaaacg cagcggaaat
     6241 accagaacct ttcagctcag gaaagtgctt actgggcgtt acgggcggct atcgaagaag
     6301 cgcgaacggc agccgagaga acgaccggct cgcggcaacc ctcgcttcgc gaggagatca
     6361 gcttgaggcg cgtcaacttc gcctatggtc ccaagataat atttcaggaa ctcgatctcg
     6421 agatccctgt taagtcgctt accactatcg cgggagcatc cggaatcgga aaaagtacgc
     6481 tccttgacct tctgtgcggg ctggcggagc cggagtcagg ggaaattctg atcgacgggg
     6541 ttccactggg ccagatcaat ccgcgggagt ggcggcgaat gatcggttat gtatcccagg
     6601 aaaacatttt gctgcatgac actatcctca acaacatact cgtaggcgca cccgatctga
     6661 aggaggctga tgcggaaaga gcattgcgtg aagcgggagc atgggatttc gtctgcgctc
     6721 ttccggaagg ggtgaggacc attgtaggcg agcgcggggg tctcctctca ggggggcagc
     6781 gccaacgcat cgccattgcg cgagccttgg ctcatcagcc tgtcctgctg ctgctggatg
     6841 aaccgacgag tgcactggat cccgaaaccg agcgcaaagt ctgcgagacc ctgcaaaaac
     6901 tcgcccgcga tatcaccgtg gtggcggtat cccaccaacc ggtgctgatc aatgcggcgg
     6961 ataacatatt tacgttatcg aaaggcaaag ccgagcggct gcaacggaca accaaggtgg
     7021 aggaagattt ttccgccatg cctgcttcgc gcatcagtgc cgtggcctcc cactgatgct
     7081 ggcgctattt tcggggctcg gttcagggag cattcaggtg gcattcagcc gctcgtcaga
     7141 tccatcccga caataacctt gctgcaaatt ccttcaacct gagctcgata ggtcgcttgc
     7201 gccactcttc cagtgtgacg agcgtggatg cgtccatatc ttttccaaac aggcttttca
     7261 tctgctcgcc gaattcctgg cctagaatga cggcgttgat ttcctggttg tgttcactgc
     7321 taagccagtc gagattggtt gaaccgatcg tcgaccacac tccatcgatc accgcagtct
     7381 ttgcatgcag cagcgcgtcc tgtcgctcgt aaatcttgac ccccgcactg agcagctcgt
     7441 catagtagga gcgtgaagcg taaaaaacaa tgttcgaatc tgttttcccg ggtaacagaa
     7501 gtcgtacgtc aacattacga cgggcagcgt ttttcaaggc ttccagcagg tcgacatcag
     7561 gcacgaaata tgcgttcgta atatacgcgt aggtctcggc gctattgatc gcggagacaa
     7621 gcgtgacata aatggggcta tagtgatcga taggacgtgt gctgatggcc cggaccactt
     7681 cagtaccctt gacaattggc ggtggaaaaa aaacttctgt tccaagcggt ccaccttttt
     7741 gtgtaaccca cgtcgtcagg aaaaattttt gaaactcggc aaccacaggc ccttcgatgc
     7801 ggatatgggt atcgcgccag ggctgagtat ttttgccgtg gggctttgac gtaaacgacc
     7861 cactcgaata cacgctgctg atattcacgc cgcctacgaa ggccacacgg ccatcaacca
     7921 caagcagttt ccggtgatcc cggcccacct cccattccct tgtcagcatg agcggattga
     7981 tgggattgaa ttccagtata cggacgcctg tatccgccaa ctgtttgaag aagctatccg
     8041 gcgtgttgga ggatccgaac gcgtcgtaaa tcaggttcac ctggacaccg cttttttgct
     8101 ttgccattaa cgctccggca aatttgcgcc cgacctcatc ggcctctatg tcgtatgtct
     8161 ccaagttaat atgatttttt gccccttgta tggctgcata catcgacttg taagtagcgg
     8221 gcccgtcaat cagaagcgtg attttattgc cagtcatcaa ggggctgccc gcgatttcct
     8281 cttcgagggc gaggtgtcgc gtaaaaatac tggtttccgg ggccgattgc ttgagtcttg
     8341 cgagaatctc ccttgtttgc tgtaccgaaa gaggtccatg cgccccctcg acaagaacgg
     8401 gaggtatcga cgatgtgggg tgccggagcg ttatacgctg ggtcagctgg gtcgcgctgc
     8461 atccgaaagt gaggagcagg cagagtaaag ccgggtactt caaaatggtc atgggtccgc
     8521 ttcctttggc tggtaatgtc aaaaaacagg gtaaccggaa gtcttctttg cattaattag
     8581 attgtatttt cgatatcgat atccttaact gtcaaatttc atatgataaa taggacgcat
     8641 ccgaaagctt cagtaagcgc ctcccgattt aataatagct catccgcaaa ttttttttag
     8701 aaaagccggg aggttatctc tcccattatt gcttctgata ccgcccgatc agctcggaat
     8761 gtccgatcac gtgaccctgc attgccttct caagctccgg cttggttgga tggttcagat
     8821 cgggcagcac gacgtcaagg gcgtagagct tatgaaaata ccggtgatta ccaatgggcg
     8881 gacacggtcc accgtaacca gttcggttcc agtcattaac gccctcgagg atgcctgggg
     8941 gaagcgcgcg ccgttctatc ccttctggca gccctggcgc gtccggcgga atattgtata
     9001 acacccagtg cacccaggtt cgttgcggcg ccgcgggatc aggggcatcc gggtcgtcaa
     9061 taatcaggac gaggctcttt gttccctgag gcacatccgc ccagaaaagt cctggcgaaa
     9121 tatcatggcc gtcgcaggtg agacgggctg gaatcgggcc gttgttcgga aaagatgaag
     9181 attcgatttt catggatgtg gctccttgct gctttttccc cgtctggctc ggttgtcctg
     9241 ccccggaatc ctcgatcacc agtccaagag agtaggtgag aatcattccg agcagcacgg
     9301 ttattgtctg aaatcggatt tttttcaagg cgcgcctgcc tgggctgctt gacagatcag
     9361 gaaaccggta tcatccgccc tgccccggta tccgacttgg gcaggcgtac cgtcaataca
     9421 ccattcgtat agctggcttc tgccttctcc gtatcgacgt tgcggggcaa aggaattgct
     9481 cgctcaaagc tgccgtaggc gcgctccatg atgtggtaag tgctgtcctg cgtcacgcgc
     9541 tcaaaccttt tttcccccct cacgtaaagg atattgccgt caatggtaat ttcgcaatcc
     9601 tttttctcca tccccggcac ttccatccgt accacgattt ccttgtccgt ttcttccacg
     9661 tctcccgcaa gcagactcca gtttctaaaa acgggaaaag cattcgcagc ctgctgcttc
     9721 tctttacctt gatcttcccg tcgtgaaaaa tgggtcagcg cgtcgctgct gtggtgaacc
     9781 agctcacgcc agccttcgga aatattatcc caggtacgac caagactccg gcttatgtgc
     9841 tttcctgcct gtttcagtgt gtcgagcacg tcatcctcct atttttaaag accctcacag
     9901 gccgaatctc attgccggaa tcttgtttcc gtctgctgct gatatctcac gagctttgcc
     9961 atcgcaagca gctcctcgga taaaagctcc agcaaatcat caagcgagag aattccaagc
    10021 agcgcacccc tctcatccac gattggcaat cggcgaacct ttctgtcttg catatactga
    10081 atcgtttcga atatgcccgt gctatccctt gcgcaggcga ggtccggttc catgatatcc
    10141 tctacggtaa taacgccggg gtcgatctca gttgcgatga tttccaccac gacatcccgg
    10201 tcggtgacca gcccgaccgg aacacgtatc ccgtcgcgct catcgaccac taccacgtcc
    10261 cccacgtgat gccgacgcat aagctttgcc gcttccagta ctgaatcgct acgtcgcaca
    10321 actatgactt cacggctaca aatttcacct ataggcataa tagcgttccg cttgttttaa
    10381 gccgatgcgt tatccgcgta gtccgggatc gattcttcga cctggcggaa gtggattgct
    10441 agttcgcagg ctcagtgatc cgattagcgc tcccaggcga cacgcagcaa atattcatcc
    10501 gggttatagt gaaagtcgag atcgccctgg tacgcgtcat gtatcgcctc tccgatcccc
    10561 cgaaccagat ggatatcggt tgtcgttagc agcatcccgt cgtcctcttc ttcaattgca
    10621 atgatccggt tgagcggatg ctcggtacgt tcccgctttt ccacatttcg cgcgagatgc
    10681 aatatttctt cacgatgggt cgcaaaaaat tgccccttca gtgtcaagaa accagcagga
    10741 tactgatcgt ttatacgatg gcaagcaggg cacatttcct gatgcgcgcc ctcgggagct
    10801 tccctccatt cccagcgccc atcgtgaaag acggcgccgc accgcggaca agtactcggc
    10861 tccttcggct tggatctcgc ccgataggga tcgtgcacgc gctcctgtaa catttcgtcg
    10921 aggcgtttga tccggtgaaa tccgggaggg ggagtggtat gggtgcccat ggaaaactcc
    10981 tgttgtcatc aattgcggct ctaaggctat gcaccctcgt acacaaaggc gcgtgtacgg
    11041 ggccggggat gatcctcatc gagcagaacg ttgggctgaa acgctcgctg tggcaagtct
    11101 cgcgctgata gccattgcct tcgcttgaat caccgcgcgt ctgctctttt ccagctcgtc
    11161 tgtgggaaaa caaaaggcag gcccgttcct ctcgggacgg gccatgtgtg gcgttaaaac
    11221 aggcaggcta tttcacctcg atccgtttgg ccgtcttccc ttccttcttt ggcagcgtca
    11281 attcaagagt gccgttatca tacttggcct cggccttgct ggcgtccact tcgcttccca
    11341 atgtgaaggt cctgtagctt gagccctgat gacactcgcg gtagagcact ctctcgcctt
    11401 ccttttcttc gttttcctgt ttgttttccg cacttatgga aacacgattc ccttccacct
    11461 gcactttcac atcctctttt tttacgcccg gaatctccgc acgcacgtga taagcattat
    11521 cgtcttccgt tacat
//
LOCUS       DASSHJ010000041         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1172191,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000041 DASSHJ010000000
VERSION     DASSHJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172191"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2547)
                     /gene="mutS"
                     /locus_tag="VFI43_01915"
     CDS             complement(<1..2547)
                     /gene="mutS"
                     /locus_tag="VFI43_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7060915.1"
                     /translation="MENLSRHTPMMQQYLRIKAQHPDMLMFYRMGDFYELFFEDAEKA
                     ARLLDITLTRRGTSAGEPIRMAGVPYHSAEQYLAKLIKLGESVVICEQVGDPAKSKGP
                     VDRQVTRIITPGTLTDSALLEEKGESLLLALCVQSSALGLSWLNLAGGQFYVMETGRN
                     GLLSELERLTPAEILLPESLDLPELGSMLASRQFNLKRLPEWQFDVEAGVRNLSSHFG
                     TRDLSGFGCEGLQASLGAASALLDYARLTQGTALDEAGQEKGLSHIQTLRVERNEAYL
                     RMDAATRRNLEISKTILGEDSPTLLSLLDSCSTNMGSRLLRHWLHHPLRDRETIQKRL
                     DAVAFLIGENGSGPYLDIRDALKRTADIERIATRIALRSARPRDLSGLRDTLKRLPAV
                     VVAVISPHDISGEIPGEADERAIHVARSMTPHPELVLLLDQALREEPALVLREGGVIA
                     DGYDMELDELRALQNNCGEFLVELEAREKARTGIANLKVEYNRVHGFYIEVTHAHTEK
                     IPLDYRRRQTLKNAERYITPELKAFEDKALSAQDRALEREKFLYETLLDLLSSHIEQL
                     QQIARSVAEMDVLATFAERALALDYAKPTFTGDAAIELESGRHPVVENQVENFIPNDT
                     RLGLLDGSGRQMLVITGPNMGGKSTYMRQVALIALLAHCGSFVPAKSARMGPIDQIFT
                     RIGASDDLAGGRSTFMMEMTEAANILNNATPQSLVLVDEIGRGTSTFDGLALAFAIAR
                     YLLENNRSFTLFATHYFELTRLTEEFPYAANVHLDAVEHKHHIVFLHAIKEGPASQSY
                     GLQVAALAGVPQSVIKTARKYLLKLERESIGSQTSNAALQRDLFSEAPVAL"
     gene            complement(2712..>3057)
                     /locus_tag="VFI43_01920"
     CDS             complement(2712..>3057)
                     /locus_tag="VFI43_01920"
                     /inference="COORDINATES: protein motif:HMM:NF018339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HET7060916.1"
                     /translation="DKWKFFDQELLANLGVLVDGHGKMPDVIIYSTVRNWLLLIESVT
                     SHGPVDGKRHDELANLFANCNAGLVYVSAFPNRKVMNKYLDVISWETEVWVADAASHI
                     IHFNGSRFLGPY"
BASE COUNT          669 a          878 c          831 g          679 t
ORIGIN      
        1 tagcgcgacc ggagcttcgg aaaacaaatc ccgctgcaat gcggcgttgc tggtctggct
       61 gccgatgctt tcccgctcaa gctttaacag gtactttctg gcggttttga tcaccgattg
      121 aggcaccccc gcgagtgccg cgacctggag tccgtagctc tggctcgctg gcccctcttt
      181 tatggcgtgc aggaacacaa tgtgatgctt gtgctctacc gcgtcgaggt gcacgttcgc
      241 tgcgtacgga aattcttcgg tcaaccgggt taactcgaaa taatgcgtgg cgaagagtgt
      301 gaagctgcgg ttgttttcca gcaggtaccg ggctatggcg aaggccagcg cgagcccgtc
      361 aaaggttgat gttccccttc cgatttcatc cacgagcacc aggctttgag gagtggcatt
      421 attcaggata ttggcagcct cggtcatttc catcatgaac gtggaacggc cgcccgcaag
      481 atcgtcagag gcgccgatgc gagtgaaaat ctgatcgatc ggccccatcc ttgcgctttt
      541 ggcgggaaca aagctgccac aatgagcaag cagcgcgatc agagcgacct gccgcatgta
      601 agtggatttc ccccccatgt tgggcccggt gatgacgagc atttgccgcc cgctcccgtc
      661 cagtaaaccc agccgcgtat cgttcgggat gaaattctcc acctggtttt ccaccactgg
      721 atggcgcccc gactcgagtt caatcgcagc gtcaccggta aacgtgggtt ttgcatagtc
      781 gagtgcgagc gcgcgctcgg caaaagtggc gagtacatcc atctcggcca cgcttcgcgc
      841 aatctgttgc aactgctcga tatgagatga cagcagatcc agcagcgttt cgtataaaaa
      901 tttctcgcgc tccaaggcgc gatcctgtgc agacagcgcc ttatcttcaa aagccttgag
      961 ttcaggcgta atgtagcgct cggcattctt gagcgtctgc cttctgcggt aatcaagggg
     1021 aatcttttcg gtatgcgcgt gggtcacctc gatatagaag ccatgcaccc ggttgtattc
     1081 caccttgaga ttcgcgatcc cggtacgtgc tttttcccgc gcttcgagtt ccaccaggaa
     1141 ttccccgcaa ttgttctgga gggcgcgcag ctcatcgagt tccatatcat agccatcggc
     1201 aatcactccg ccctcacgaa gcacaagcgc aggctcttcg cgcaacgcct ggtcaagaag
     1261 caatacaagc tcgggatggg gcgtcatcga tcgagccacg tggatggcgc gttcatcagc
     1321 ttccccgggt atttccccgc taatatcatg aggggaaatg acagccacta caacggcggg
     1381 caatcttttg agggtatcgc gcagccctga caaatcgcgc ggacgggcgg atctaagcgc
     1441 aatgcgggta gcgatgcgct cgatgtcggc tgtacgcttc agtgcgtccc gtatatccag
     1501 gtagggccct gatccattct ccccgatcag gaacgcaacg gcatccaggc gcttttggat
     1561 ggtttcgcgg tcgcgcaacg gatggtgcag ccagtgccgc agcaggcggc tgcccatgtt
     1621 cgtggaacag ctatcgagca gggagagcaa cgtcggggag tcctcgccca gtatggtttt
     1681 cgaaatctcc aggttccggc gggtggctgc gtccatgcgc aagtaagcct cgttccgctc
     1741 cacgcggagc gtctgaatat ggctcaggcc tttttcttgc ccggcctcat cgagggctgt
     1801 gccctgggtc aggcgtgcat agtcgagcag agcgctggct gcgccgagtg atgcctgcaa
     1861 gccctcgcaa ccgaatcctg aaaggtcgcg ggtaccaaaa tgagaagaca gattgcgtac
     1921 acccgcctcg acatcaaatt gccactcggg gagacgcttc aagttaaatt gccgggatgc
     1981 gagcatactg ccaagctccg gaagatccag agattcggga aggagaattt ccgcgggcgt
     2041 gagccgctcc aactcgctca acaagccgtt gcggccggtt tccatgacat aaaactgacc
     2101 cccagccaag ttgagccatg acagtcccag cgccgagctt tgcacgcata aggcgagcag
     2161 caggctttcg cccttttctt cgagaagcgc cgagtccgtg agcgtgcccg gcgtgatgat
     2221 ccgcgtgacc tggcgatcca ccggcccctt gctcttcgcc gggtccccca cctgttcgca
     2281 aatcactacc gattcaccca gcttgatgag ctttgcgaga tactgctcag ccgagtgata
     2341 gggcacgccc gccatccgga tcggttcacc cgcagacgtt ccccggcgcg tcagggtgat
     2401 gtcgagcagc cgggcggctt tttccgcatc ctcaaagaac aactcgtaaa agtctcccat
     2461 gcgataaaac atcagcatgt ccggatgctg cgccttgatg cgcagatact gctgcatcat
     2521 gggggtgtgg cggcttagat tttccatttt gtcatcaagc tacgacaaaa gaaaaagatt
     2581 ttacttagac gcaaaaaatc aaccttgccg aaagctctca aacactcttt aaacgtcaaa
     2641 gcctcttctt tgcccagcga tctaacatct aacgccggaa tcggtgttta tcttgcagta
     2701 gcaccacgac ttcagtacgg gcccagaaag cgggagccgt taaaatggat aatgtgggag
     2761 gccgcatctg ccacccacac ctcggtttcc caagaaatga cgtccagata cttattcatt
     2821 accttccggt tgggaaaagc cgacacatag acgagaccag cgttacaatt tgcaaataaa
     2881 ttagcgagct catcatggcg cttaccgtca acaggtccat ggcttgttac tgactcgatt
     2941 aaaaggagcc aatttctgac cgttgagtag atgatgacat caggcatttt tccatgaccg
     3001 tcgaccaaga ctccgaggtt tgctaaaagc tcttggtcga aaaacttcca tttatcg
//
LOCUS       DASSHJ010000042         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1279000,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000042 DASSHJ010000000
VERSION     DASSHJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279000"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFI43_01925"
     CDS             <1..578
                     /locus_tag="VFI43_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HET7060917.1"
                     /translation="NAEAQTKLAMIKDLFAKRSRVAAVSPPATPVPVPGSSVAIEEKP
                     SSKPDHKSAAKTEKPAEKIASVEKLESAKPDVQGESGEILDTITAWAKAWSDKDVPTY
                     LGFYSSEFRAPGSASRNAWEKTRRDRIIKPKSIEVSIANPKVSFTDATHAKVSFRQSY
                     HSDAIKSNTTKTLEMVKNGQKWQILHEQVGR"
     gene            575..1825
                     /locus_tag="VFI43_01930"
     CDS             575..1825
                     /locus_tag="VFI43_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7060918.1"
                     /translation="MMKPDALIGTGARVIKPVTAARMLMAGLFYLGASCVLASGPTLP
                     REPHQSAPEAMLMKSLEQIAESRMDSAMSGIDELLKSNPNFRLAHLIKGDLLLARTRP
                     ISSIGNAPQAPEQHVADLREEARVRIQRHREQVPRDRVPRYLLQMQPEQKYAVIADTD
                     KSRLYVFQNVGGKPRYIADYYISTGKNGSVKIKEGDKKTPIGVYFVTANVPREKLTDF
                     YGRGAFPLNYPNEWDKRNGRSGFGIWLHGTPSDTYSRPPRASDGCVVLANQDLDRVGA
                     TLQVGVTPVIISNAVEWVDPADTIVLRDKIASNLESWRRDWESGNTQSYIRNYRKDFS
                     TGSQDLAEWSKHKRQVNAGKNWIKVGISNVGMFLYPGRDDLVVVNFDQEYSSNNLSNK
                     MRKRQYWLRESKNGPWKIIYESAA"
     gene            1866..>2243
                     /locus_tag="VFI43_01935"
     CDS             1866..>2243
                     /locus_tag="VFI43_01935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7060919.1"
                     /translation="MHTRLVAAFFTFLSLFLSIPASAANPQVELKTNFGNITIELYPE
                     KAPKTVENFVGYIKDGYYNGTIFHRVIPGFMIQGGGFDKSFKQKPTRPPVQNEAANGL
                     KNEVGTVAMARTSDPHSASSQFFI"
BASE COUNT          604 a          593 c          608 g          438 t
ORIGIN      
        1 gtaatgccga ggcccagacc aagcttgcga tgatcaagga tctgttcgcg aaaaggtcaa
       61 gggttgcagc tgtctccccg cccgccacac cggttcccgt gcccggttct tccgtagcga
      121 ttgaggaaaa accatcttcc aagccagatc acaagagcgc ggcaaagacc gaaaaacccg
      181 cggagaaaat agcttccgtc gaaaaacttg agtcggccaa gccggatgtg caaggagaat
      241 ccggcgaaat actcgataca atcacagcct gggccaaggc atggtcagac aaggatgtgc
      301 ccacctacct cggattttac tcgagcgaat ttcgcgcgcc cggaagcgca tcacgcaacg
      361 cgtgggaaaa gacccggcgg gacaggatta tcaaacccaa atcaatagaa gtatcgatag
      421 caaatccgaa ggtcagcttt accgatgcaa ctcatgcaaa agtgagcttc aggcagtcct
      481 accactctga tgcgatcaag agcaatacga cgaagaccct tgagatggtc aagaatgggc
      541 aaaaatggca gatactgcac gagcaggttg gtcgatgatg aagcctgatg ccctgatcgg
      601 gacaggggcg agggtgatca agcctgttac tgcggcgcga atgctgatgg caggcttgtt
      661 ttatcttggc gcttcctgcg tcctggctag tggccctacg ctcccccggg agcctcatca
      721 aagcgctcct gaagccatgc tcatgaagag tctcgagcag atcgcggaaa gtcgaatgga
      781 ctctgccatg agtggaatcg acgaactgct gaaatccaac ccgaacttcc ggcttgccca
      841 tctgatcaag ggagatttgc tgcttgcccg gacccgccct atcagcagca ttggaaatgc
      901 gccgcaagca ccggagcagc atgttgctga cctgcgcgaa gaagcgcgag ttcgaatcca
      961 gcgtcatcgc gaacaggttc ctcgcgatcg cgtgcctcgg tatctcctgc aaatgcagcc
     1021 cgagcaaaaa tacgccgtca ttgcggacac ggacaaatca cgcctttatg tattccagaa
     1081 tgtgggcggc aagccgcgct atatcgccga ttactatatc agcaccggaa agaacggctc
     1141 agtcaagatc aaggaaggag acaagaaaac gccaataggc gtatattttg taaccgccaa
     1201 tgttccccgg gaaaaactca ctgattttta cggccgcggc gccttccccc tcaattatcc
     1261 caatgagtgg gataagcgta acggaagaag cgggttcggc atctggctgc acggcacccc
     1321 ctcggacact tatagccgcc cgcctcgcgc aagcgatgga tgtgtagttc tcgcgaacca
     1381 ggacctggac agggtgggcg ccacgctgca ggtgggcgtc actccggtga ttatcagcaa
     1441 tgccgtggaa tgggtggacc cagcggatac gatcgtgctg cgtgacaaaa tagcaagcaa
     1501 cctggaaagc tggcggcgtg actgggaaag cgggaacacg cagtcataca tcagaaacta
     1561 ccgaaaggat ttttccacgg gcagccagga cctggcggaa tggtcgaaac acaaacgtca
     1621 ggttaatgcg gggaaaaact ggatcaaggt gggaatcagt aatgtaggaa tgtttctata
     1681 tccagggaga gatgatctgg tagtggtcaa tttcgatcag gaatactcca gcaacaatct
     1741 ttcaaacaag atgagaaaac gccagtactg gctaagagaa agcaagaacg gcccgtggaa
     1801 gattatttat gagagtgcag cgtgaatccc cggacacgcg gcttcaaacc acttattacc
     1861 taatcatgca taccaggctt gtagccgcat tttttacctt cctatccctc tttttgagta
     1921 ttcctgcgag cgccgcgaat ccccaggttg aattaaagac caatttcggt aatatcacga
     1981 tagagcttta cccggaaaag gcgccaaaaa ctgttgagaa cttcgttggt tacataaaag
     2041 acggctatta caacggcacg atatttcatc gcgtcatccc gggattcatg atccaggggg
     2101 ggggcttcga caaatcgttc aaacagaagc cgacgaggcc gccggttcag aacgaagcgg
     2161 cgaacgggct gaagaacgag gttggcaccg tggcaatggc ccgcacttca gacccgcact
     2221 cggcatcctc gcagttcttc atc
//
LOCUS       DASSHJ010000043         7783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_516169, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000043 DASSHJ010000000
VERSION     DASSHJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516169"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFI43_01940"
     CDS             complement(<1..1126)
                     /locus_tag="VFI43_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7060920.1"
                     /translation="MKLGKILCLGLISNPLCLAVANDVPALPELTIYGGNRIPMDREL
                     TNHRVNTVRLKVEDNDWKTVLQEQVQINELGPGGPLALFMRGSNSNQAVFLLDGVKMS
                     SATTGSPNFAAIAPGLLSSVDVIQGGVSAAFGSNAVGGVIRGNIDIPVGMLVSAGGGS
                     RFSERAAASLGVRGESFYAGIRVVQDIIQQGSATNSNSVFSFNPDKDPRRRIGVVAKA
                     GGWITPDFQVEATALGSGTRTSFDASPTTDDVNRQSIAMANVRAIYSLPHDLSIMGTY
                     GTSLDRSNVLGAFPAIFNSRSLQGSLQLEKKLTSDKLTGRVFAGMDHEIQSVGSTTEF
                     TKKSRTNVAGFVGTQLNLGEHVAEAMYRHDENSQFGDFNTY"
     regulatory      complement(1392..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1833..2033
                     /locus_tag="VFI43_01945"
     CDS             1833..2033
                     /locus_tag="VFI43_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060921.1"
                     /translation="MEPELKALEDKIHQFVLLCQQLRSENIGLRQQLAAAISENKRLT
                     EKISSATGQLEAILAQFPESEE"
     gene            2030..2338
                     /locus_tag="VFI43_01950"
     CDS             2030..2338
                     /locus_tag="VFI43_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7060922.1"
                     /translation="MKSGKAIDISIMGRDFCITCPDEEREELLKAVAYLDKKMREIRD
                     AGKVIGSERIAIMAALNITHELLIARHKEGFDTEEFRRRIDCMQAALDAAMPGQENLF
                     "
     gene            2421..2597
                     /gene="ssrS"
                     /locus_tag="VFI43_01955"
     ncRNA           2421..2597
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFI43_01955"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2768..4258
                     /locus_tag="VFI43_01960"
     CDS             2768..4258
                     /locus_tag="VFI43_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7060923.1"
                     /translation="MPREPADIRNDPEGMRLPIKLDSTSNGEFEPVPLTPANQAANHL
                     ASQAASDNAKRLGLSRRKFLISACGAASTLLAFNRANAAAGRTGGFFDLPSTAALEPT
                     LAQSRIEGEKSEFIFDVQGHFVDPNGDWVKKLPGGSNPLSQMPKAGCALASGPESRSY
                     LRCLGPDEFIKDVFLDSDTDMMVLSFVPSKPDAEPLTIQTADAVRRIIDRMEGMHRLL
                     LHGRVNPNQPEDLESMDELKERWGVSAWKTYTQFGPGGKGYFLSDDIGTRFIEKARKL
                     GVKVICVHKGLPFGKQSYEHSKCTDIGVVAKRFPDVAFLVYHSGFVTSVPERAFTGRG
                     AGDGIDTLIQSLIENGVPPNSNVYAELGSTWRFLMRDPEAAAHALGKLLKYCGENNVL
                     WGTDSIWYGSPQDQIQAFRTFQIAREFRERYGYPEITPGLRANIFGLNAAKVYSISPD
                     EIKKYSGRDRIAEERQAYLEQPDPHFLTYGPKTRRDFLRLTAAGGF"
     gene            4964..5410
                     /locus_tag="VFI43_01965"
     CDS             4964..5410
                     /locus_tag="VFI43_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7060924.1"
                     /translation="MEEKPKPRIVGINHVALEVGNIEEALAFYSRIFDFNLRGRGSGS
                     AFIDMGDQFIALMEGRSHPTNDERHFGLVVDDRSRVRELAAAAGAKLLEGGRMDFLDP
                     WGNHIQVVEYRDLQFMKTESVLKFMGLHLDKTEAAQAELRQKGIEP"
     gene            complement(5501..6835)
                     /locus_tag="VFI43_01970"
     CDS             complement(5501..6835)
                     /locus_tag="VFI43_01970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET7060925.1"
                     /translation="MPFFQESNKKPGENSALSSGLNYKLGRRKFVFYALSAGLAVVAH
                     SFSTQSSAQSAKEGNSAPVMNDQDGVNLLPQDESLRVINIFLCGDVMTGRGIDQVLPY
                     PGNPILYEGYMKSAVGYVQLAEAKNGPIPKPVDFSYIWGDALDELERRKPDLRIINLE
                     TAVTRSNDVQDKAVNYRMNPDNIPCILAAKIDCCALANNHILDWGYAGLAETLDTLKK
                     VGIKTPGAGRNLGEARAPALMPVSRKGRILVFSFGSETSGIPWSWGAKPDKPGVNLLP
                     DFSLTTVKEIRRQIGQEKRTGDVVVASIHWGGNWGYQVPHEQQEFAHWLIDDGGVDIV
                     HGHSSHHVKGIEVYRGKLILYGCGDFLNDYEGISGHESYRGDLGLMYFAAVEPANGKL
                     VRLTMVPMQMKRFRLNYASKKDVAWLKDVLDREGHKFGTAVEVVSNTMLLRW"
     gene            6877..7323
                     /locus_tag="VFI43_01975"
     CDS             6877..7323
                     /locus_tag="VFI43_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HET7060926.1"
                     /translation="MRLWTIHPCYLDPQGLTAAWREALLAQKVLTDTTKGYRAHPQLV
                     RFRLHPQPLAAIGVFLFALAEEAERRGYKFETKKILEKEPVEQIEETEGQLFYEWAHL
                     RAKLHKRAPDLHRQFHSIVIPQSHPLFRIVPGKIREWEKSRSIRID"
     gene            complement(7479..>7783)
                     /locus_tag="VFI43_01980"
     CDS             complement(7479..>7783)
                     /locus_tag="VFI43_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060927.1"
                     /translation="HVFPDTPQSDLERAEANLYYVQVAVPFSKTELNENSIRLSIKGD
                     DAWLPDRLFLFGLDEDETGPAAGFVVPLVHLPEWPLGTLSTDPEEGQGSVVLPLSM"
BASE COUNT         1930 a         2123 c         1992 g         1738 t
ORIGIN      
        1 aataggtgtt gaagtccccg aactggctat tctcgtcgtg ccgatacatc gcctcggcaa
       61 cgtgttcgcc taggttgagc tgggtgccca caaatcccgc gacgttcgtc cggcttttct
      121 ttgtaaactc ggtcgtactg ccaaccgact gaatctcatg atccatgccg gcaaagaccc
      181 gaccggtgag cttgtcggac gtcaactttt tttccaactg aagcgaaccc tgcagagagc
      241 ggctgttgaa tatcgcgggg aacgcgccta gcacattgga acggtcgagc gaggtgccat
      301 acgttcccat aatgctgaga tcatgaggaa gcgagtagat cgctcttacg ttggccatcg
      361 ctatggactg gcgattcacg tcgtctgttg tgggcgaagc atcgaacgac gtcctcgtgc
      421 cagagcccag cgccgttgcc tcgacctgga aatccggagt tatccatcct ccagcttttg
      481 cgacgacgcc aatacgccta cgagggtcct tgtccggatt gaagctgaaa acactgttac
      541 tgttcgtggc tgatccctgc tgaataatgt cctgcaccac gcggataccc gcataaaacg
      601 actctccccg tacccccagg ctagccgccg cccgctccga aaatcgcgag ccgccccccg
      661 cagataccag catccccacc ggaatatcaa tgttgccgcg tatcacgccg cccaccgcgt
      721 tggacccgaa cgcggccgac actccccctt gaataacatc gacggatgac aagagacccg
      781 gcgcaatggc ggcaaaattg ggtgagcccg ttgttgccga agacattttg accccgtcca
      841 gcagaaatac cgcctggttt gagttggaac cacgcatgaa gagcgctaac ggcccacccg
      901 gcccgagctc gttgatctgc acctgctcct gcaacaccgt cttccagtcg ttatcttcga
      961 ccttcaatct gaccgtgttc accctatgat tcgtgagctc acgatccatc ggaatccggt
     1021 taccgccata gatcgtcaac tccgggagag ccggcacatc gtttgccaca gccagacaaa
     1081 gcggatttga tatcaatccc aggcacaata tttttcccaa tttcatcttg atccttccag
     1141 ccaagcctgg cgtaaatttc ccaccccaaa tccgcctgct cgcaaaaatg caccggatca
     1201 cgacagcaca tttgcggatc gtttaatccg cagcttattt gccaatgggt aatatttcag
     1261 atacgggcag tgcaggcagc ccggatcgaa ccgcggaaac tacgtgggag taaaatgaaa
     1321 ggtcaaaagc cgcgcgacat cgcaaggata tcgataagcg gcttggctat cagcacaccg
     1381 ccccacgcgg catttccaga tgcggtcaag gccggtatcc gggcttgcag gacttgatgt
     1441 atcgccttcc catgaattgg aggattcaca gtggctcggg gatgccatgc tggaggacat
     1501 ccgatacacc cacacctgct taccgttgcg ggggcagccc aggcatttca aggaagctct
     1561 gaaaaaacct tttaacagtg cattcctaac ctggttcccg tttaacttgg ccgtttatgc
     1621 agccaggcac cttgaacgag ccgaattcta ccagaaccgt tgctcggcag ttgaaaatcg
     1681 ggtgatacgg aaggcaggag gacaattatc aagaaagggt acgctcaggc tgtgagatgc
     1741 tcgaaattac tttcagagag tcaccttaac agttgacgca gtaaggaggc ccgaactata
     1801 gtgcgccagc agaatagaag aaatataagt agatggaacc tgaactgaag gctctcgaag
     1861 ataaaattca ccagttcgta ctgttgtgcc agcaactgcg atcagaaaac atcggcttgc
     1921 gccagcaatt ggcagcagcc ataagcgaga acaagcggct gacggaaaaa atcagctctg
     1981 caaccggtca gctcgaagcg atactggccc agtttcctga gagcgaagaa tgaaaagcgg
     2041 caaagccatt gatataagca tcatgggacg ggatttttgc attacctgtc ctgacgagga
     2101 acgcgaggaa ctgttgaaag cagtcgcata tctcgacaaa aagatgcgtg aaatcaggga
     2161 cgcgggaaaa gtgatcggat ccgaacgtat cgcaatcatg gctgccctca acattacgca
     2221 tgagctgttg atcgcccggc acaaggaagg ctttgacacg gaggaattta ggcgtagaat
     2281 cgattgcatg caggctgcgc ttgatgccgc gatgccagga caagagaact tgttctgatt
     2341 cacatccgaa tgcaggccag tccgaacgat ttccggcctt cgttgctcga tcgtttcaac
     2401 gaacgaagat gggtaaaggt tgttccctgc ggtgttcgtt ttaggcctat attctttgaa
     2461 ccaactttag cattggttgc aaaccaaagt gatgctgttg tgcgcgtccc atgcggaagc
     2521 gcctgatgca tccggtgagt gaccaccttg aaccatctgg ttcaagataa tggcctgacg
     2581 gcatttgcgg ggaccttgtt ttccttggca acttctgaat aatcctcagg gatcaggatt
     2641 attcagaaga tttcccagaa accccggttc aagtttgcgg aatctgaccg ctttagaagt
     2701 accttgagga tgtgcgcgcc cgcgataacg tcttttccca gctctgcatt tatcgccatt
     2761 acaggaaatg ccaagggagc ccgctgacat tcggaacgat cctgagggaa tgcgccttcc
     2821 gatcaagctc gattccactt ccaatggcga gttcgagcct gttcccctga cacccgccaa
     2881 tcaagccgca aaccaccttg cctctcaagc ggcaagtgac aacgccaaac gcctgggtct
     2941 gtcccgaaga aaattcctga tatccgcttg cggtgctgcc tccaccttgc ttgcattcaa
     3001 cagagccaat gcggccgcgg gcagaactgg aggtttcttc gatcttcctt ctacagccgc
     3061 ccttgagcca accctcgccc agtcccgaat cgagggtgaa aaaagcgagt ttatcttcga
     3121 cgtacagggt cattttgtcg acccgaacgg agactgggta aagaagcttc ccggcggctc
     3181 gaaccctctg agccaaatgc ctaaagccgg ctgcgccctg gcctcgggac cagaatcgcg
     3241 aagctatttg cgctgcctgg gacccgacga attcatcaag gatgtgtttc tcgactccga
     3301 caccgacatg atggtgctct cgtttgtgcc ctccaagccg gacgccgagc ccctaacgat
     3361 acagactgcg gatgcagtgc ggcgaattat cgaccgtatg gaaggaatgc atcgcctgct
     3421 tcttcacggc cgcgtcaacc ccaaccagcc agaagacctc gagtcgatgg atgagttgaa
     3481 ggaacgctgg ggcgtgtccg catggaagac ctatactcag tttggccccg gcgggaaggg
     3541 ttacttccta tccgacgata tcggaacgcg attcatcgag aaagcccgca agctcggcgt
     3601 gaaggtcatc tgcgtgcaca aggggttacc cttcggaaag cagtcttacg agcacagcaa
     3661 atgcaccgac attggggtcg tggcaaagcg gtttcctgac gtcgcttttc tcgtttatca
     3721 ctcaggcttt gtcaccagtg tgcctgaacg tgcattcaca ggaagagggg cgggagacgg
     3781 catcgatacg ctcatccagt cgctcatcga gaatggcgta cctccgaaca gtaatgtgta
     3841 cgccgagctc ggaagcactt ggcgtttcct gatgcgggac cctgaagctg cggcccatgc
     3901 gttgggaaag ctcttgaaat actgcggcga gaacaatgtc ctgtggggca cggattccat
     3961 ttggtacggg tcgccacaag accagatcca ggcatttcgg acgttccaga ttgcgcgcga
     4021 atttcgggaa aggtatggat atcccgagat tacaccgggg cttcgcgcaa acatttttgg
     4081 actcaacgcg gcaaaggttt actcgattag ccccgacgag ataaagaaat atagtgggcg
     4141 cgaccggata gccgaggaac gacaggctta tctcgaacaa cctgacccgc acttccttac
     4201 gtatggacct aaaacccgaa gggattttct gcgattgacg gcggctggcg ggttttgaca
     4261 cactcgttgt catcttatct actcgctacc catcaccgtc atgcaggcgg gaatcgagcc
     4321 cacacctctt ctgcttctca tatccgtgca agcggaatcc gacccttccc tcttctgtct
     4381 cgtcatcccc ggcgaagcgg gaatcaagct tccccctctg cgtcattacc acgaagcagg
     4441 ttgtgaaaaa actcgagaaa acagcacctc aatcgaaaat attcctactt atatgctcac
     4501 tactcgacat taataggttc cgcataccac ttagatttcg gtcatattcg caaaactcac
     4561 ttgccttccc acctcggaaa atccttcgag agttttttca aaacctgttt cgggggaatg
     4621 acgaagggaa cgtgtagagc tgggggcacc gttgggaata acacacaaca attatcgtct
     4681 gatgcgctat tttgaacctt ccatcaacgt cgtgagcaag gacaactatt atccggttag
     4741 gaaacggagc ggaatctggc gcaatagcgc ggcacgcaac agcctcttca atttcccgtc
     4801 ccagctccat gaaattgctg tagatgaaaa aaccatgagg attacatcta tgctataggt
     4861 gacgtttttt tgccatagct ctgacccaat gccgaaaagg aaatggcaaa cgtaaaaaag
     4921 aaaaacagca attcgggtgg caatcactcc aaggaggagg aatatggaag agaaaccgaa
     4981 accgcgcata gtcggcatta atcacgttgc cctggaggtc ggcaatatcg aggaagcgtt
     5041 ggctttctat agccgcatct tcgacttcaa tttacgaggc cgcggcagcg gttccgcctt
     5101 cattgatatg ggcgatcaat ttattgcgtt gatggaagga agatcgcatc cgacgaatga
     5161 cgagcggcac ttcggcctgg tcgtggacga ccgttcaaga gtgcgagagc tggctgccgc
     5221 ggccggcgca aaactgctgg aaggcggcag gatggacttt ctcgacccgt ggggcaacca
     5281 cattcaagtg gtggaatacc gcgatctgca atttatgaag acagagtcgg tactgaaatt
     5341 catggggctt catctggata aaacagaggc cgcccaagcc gagctgcggc aaaaaggaat
     5401 cgaaccttga ggcggggttc aaataagaac cgaatatgag agggggtata aaaagctttg
     5461 aacaggattg cggcaaggca agccttcacg ctgaagagtg ctaccagcgt aacagcatcg
     5521 tgttggaaac aacttccacc gccgtcccga atttgtgccc ttccctgtcc aaaacatctt
     5581 tgagccaggc aacatctttc tttgaagcgt aattcagcct gaagcgtttc atctgcattg
     5641 gaaccatggt gagccgcacg agcttgccat ttgccggctc gacagcagcg aaatacatca
     5701 gcccaagatc gccccggtag gattcatgtc cggaaatgcc ttcatagtcg ttcaggaaat
     5761 ccccgcagcc gtaaaggatg agttttcccc gataaacctc aatccccttc acatggtggg
     5821 aagaatgacc gtggacgatg tcgacgcctc catcgtcgat cagccagtgg gcaaactcct
     5881 gctgttcatg aggtacctgg tacccccagt ttccacccca gtgaatagag gcgacaacga
     5941 catcacccgt tcttttttcc tgcccaattt gacgcctgat ttcctttacg gttgtcaggg
     6001 agaagtcagg caaaaggttc actcctggtt tgtcgggctt ggcaccccag ctccatggta
     6061 tgccgcttgt ttccgaaccg aatgaaaaaa cgagaattct tcccttccgg gaaacgggca
     6121 tcaaggcagg agctcgagct tcccccaggt tccggcctgc ccccggcgtc ttgattccca
     6181 ctttttttag cgtatccaac gtttcagcga gtccagcata tccccaatca agaatatgat
     6241 tgttggccaa ggcgcaacaa tctattttgg cggcgaggat gcaggggata ttgtccggat
     6301 tcatccggta attgaccgcc ttgtcttgaa catcgttact tcgcgttacg gcggtttcca
     6361 gattgatgat gcgcaggtca ggcttgcggc gctcgagttc atcaagggcg tccccccaga
     6421 tatatgaaaa atcgaccggc ttgggaatcg ggccgttttt ggcttcagcc agctgaacat
     6481 acccaactgc gctcttcatg tagccttcgt acaagatggg gttgcccgga tagggcaaga
     6541 cttggtcgat tccccgccct gtcatcacgt ctccacatag aaatatattg attacgcgca
     6601 ggctctcgtc ctgtggaagc aaattgacac cgtcctggtc gttcatgaca ggagccgagt
     6661 tcccttcctt tgcggattgc gccgaagatt gggtggaaaa tgaatgagcc acaaccgcaa
     6721 gtccggccga aagcgcataa aaaacgaatt ttcgccgacc aagcttatag ttaaggccgc
     6781 tactcagtgc cgaattttcc cccggttttt tgttcgattc ttgaaagaat ggcatgcttc
     6841 catcctagac tatattcaga agaaacactg tttgagatgc ggctctggac tattcacccc
     6901 tgctatcttg atccgcaagg cctcacggct gcgtggagag aggcattatt ggctcaaaag
     6961 gtgcttaccg atacaacaaa gggatatcgg gcacatcccc aacttgtgcg gttcaggctg
     7021 catccgcagc cacttgccgc aataggggtc tttctattcg ctcttgcaga ggaagctgaa
     7081 cggcgaggct ataaattcga aacaaagaag attcttgaaa aagagcccgt agagcaaata
     7141 gaagagacag aggggcagtt attctacgaa tgggcgcact tgagagcgaa actgcacaag
     7201 cgagcgccag accttcaccg acagtttcat agcattgtta tccctcagtc ccacccgtta
     7261 tttcgcatcg ttccaggaaa aatcagagag tgggaaaaat cacgatcaat acggatcgat
     7321 tagcatcaag ggttgcgggc gcatgggctg gagagcactt cgctgggggc tcggccctcg
     7381 ccgttcccag ttcctggaca cagcacaacc gctcgtcccc ggcagctgca acgaaaaacg
     7441 gtcatcgttc gtgttcaaca attgttatct cttcacgatt acatcgacaa aggcaataca
     7501 acggatccct gcccctcctc cggatcggtg ctcagcgtcc cgagtggcca ttcagggaga
     7561 tgcacgagag gcacaacaaa tccggcggct gggcccgttt catcctcgtc aagtccaaaa
     7621 aggaataagc gatccggcaa ccacgcatcg tcacctttga tacttaaacg aatcgaattt
     7681 tcattcagtt cagttttact gaaggggaca gcaacttgca cgtaatagag attcgcttcc
     7741 gccctttcca gatcgctctg cggcgtgtcc ggaaaaacgt gat
//
LOCUS       DASSHJ010000044         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_79438, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000044 DASSHJ010000000
VERSION     DASSHJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79438"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFI43_01985"
     CDS             <1..386
                     /locus_tag="VFI43_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET7060928.1"
                     /translation="GRSNIVGKPMALMLLQKGATVTICTSKTRDLGEHTRRADILVVA
                     VGKPGTVTAEMVKRGATVIDVGINRMPDGKITGDVDFESVKEKAANITPVPGGVGPMT
                     ITMLLGNTVEAAERAYKRKTAPIQA"
     gene            598..3261
                     /gene="aceE"
                     /locus_tag="VFI43_01990"
     CDS             598..3261
                     /gene="aceE"
                     /locus_tag="VFI43_01990"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET7060929.1"
                     /translation="MEPQPDVDPTETQEWLDAIQSVLTYEGTERAHYLLERLIEKARR
                     SGAYLPYSATTAYINTIPPGKEEPSPGNHALEHRIRSYVRWNSMAMVLRANRHSNVGG
                     HIASFASAATLYDVGYNHFWHAASDKHAGDLIFAQGHSSPGLYAYAFLLGELTEEQLD
                     NFRREVGGKGLSSYPHPWLMPDFWQFPTVSMGLGPLMATYQARFMKYLDSRGLVNTQG
                     RKVWAFMGDGEMDEPESMGGISLASREKLDNLIFVVNCNLQRLDGPVRGNGKIIQELE
                     AAFRGSGWNVIKVIWGSYWDPLLAKDTKGLLQQRMMECVDGEYQTFRARDGAYVREHF
                     FGKYPELLAMVANMSDDDIWRLNRGGHDPHKVYAAYAAAVKHTGQPTVILAKTIKGYG
                     MGEAGEAQNITHQQKKMGTTSLKAFRDRFALPIPDDKMDEVPYLKFDKDSPEYIYMHE
                     RRTALGGFLHRRRGKAEALQVPPLSAFENLLKASGEGRESSTTMAFVRILNTLVKDKN
                     IGKRVVPIVADESRTFGMEGMFRQLGIWSSTGQLYTPQDADQLMYYKEDKNGQILQEG
                     INEAGAMASWMAASTSYSTHGVQMVPFYIFYSMFGFQRVGDLAWAAGDMRCRGFLLGG
                     TAGRTTLNGEGLQHEDGHSHLAASTIPNCISYDPTFAYELAVIIHDGLRRMNEQQEDV
                     FYYITVMNENYSHPEMPAGAEDGILKGMYLFRESKTAEEAGLRVQLLGSGTILREVIA
                     AGDILEKEFGVVADIWSVTSFNEVRREALDVARWNMLHPASEPRISHVTACLKDRQGP
                     VVAATDYMKIFADQIRAFVPGRYKVLGTDGFGRSDTREQLRLFFEVNRYYITIAALEA
                     LAEDGKMPIEKVTQAIEKFGINPDKPNPATV"
BASE COUNT          821 a          846 c          983 g          698 t
ORIGIN      
        1 ttggccgcag caacattgtc gggaagccga tggccctgat gttgttgcag aaaggggcga
       61 cagtgacaat ctgtacgtcg aagacgcgcg acttgggaga acatacccgg cgtgccgata
      121 ttctcgtggt tgctgtcggg aaaccaggaa cagtaacggc cgaaatggtg aagcggggcg
      181 cgaccgtgat cgacgttggc ataaaccgca tgcctgacgg taaaatcacc ggtgatgtgg
      241 atttcgaatc agtaaaggaa aaagcagcaa acatcacccc tgtaccgggc ggagtcggcc
      301 caatgaccat tacaatgttg ctcggcaaca cagtggaagc ggcagagcgg gcgtacaagc
      361 ggaaaacagc cccgatccaa gcgtgatttt atagcgggtg aactggcggc ccacaatgca
      421 tatgggcgag ctttcgggtg aagcagcgta tttggtttcc tgttgaagga catcccgcta
      481 ccggtattct ggatgcaact ttgctgaagg ctgccagtaa acaggttgaa gtttgctgga
      541 agtttcccga atctaagcaa tcggtgacgt gaaattagcc gagaacgcga gacaaacatg
      601 gaaccccaac cggacgtaga tcccactgaa acccaggaat ggctcgatgc gatccaatcc
      661 gttcttacct acgaaggtac ggaaagagcc cattatcttc tcgaaagact gatcgaaaaa
      721 gcccggcgct caggagccta ccttccttac agcgccacta cggcttatat caatacgatc
      781 ccacccggca aggaagagcc atctcctggc aatcacgccc ttgagcatcg aatacgctca
      841 tatgtacgct ggaactcgat ggcgatggtg ttgagagcca atcggcactc gaatgtcggg
      901 ggacacatcg ccagttttgc gtcagccgcc acgctctatg acgtgggcta caaccatttc
      961 tggcatgctg catccgacaa gcatgccggg gacctcatat tcgcacaggg ccattcgtct
     1021 ccaggacttt atgcatatgc tttcctgctt ggcgaattga ccgaggagca actcgataac
     1081 ttccggcgcg aggttggagg aaaagggctt tcatcatatc cccacccctg gctcatgccg
     1141 gatttctggc aatttcccac ggtctccatg ggtcttgggc cgcttatggc gacttaccag
     1201 gcccgcttta tgaaatatct ggatagccgg ggtcttgtca acactcaggg acggaaagtc
     1261 tgggccttca tgggtgatgg agaaatggac gagccggaat cgatgggagg catttctctc
     1321 gcctcgcgcg agaaactgga taacctgatc tttgtggtga actgcaacct ccaacgtctg
     1381 gatggacctg tacgaggtaa tggaaaaatt atccaggaac tcgaagccgc gtttcgaggg
     1441 tcaggatgga atgtcatcaa ggtaatctgg ggatcctact gggatccgct cctggctaaa
     1501 gacaccaagg gactcctgca gcaacgcatg atggaatgcg tggatggcga gtaccagacg
     1561 ttcagggcac gggatggtgc ctacgtgcgc gaacactttt tcgggaagta tcctgagctg
     1621 cttgcaatgg tggccaatat gtcggatgac gacatctggc gcttgaatcg cgggggacat
     1681 gatcctcata aagtgtacgc agcgtatgcc gcggcagtaa agcataccgg gcagccgacc
     1741 gtgattcttg cgaaaacgat caagggctac gggatgggcg aagcaggcga agcccagaat
     1801 attacccatc aacagaaaaa gatgggcacg acgtcgctga aggccttccg ggaccggttc
     1861 gcgttgccca ttccggatga caagatggac gaagtccctt atctgaaatt cgataaggac
     1921 tcgcccgagt atatctatat gcatgagcgg cggactgcgc tgggaggctt tttgcaccgc
     1981 cggcgcggta aagcggaggc actacaggta cccccgctgt ccgcgtttga aaacttgctc
     2041 aaggcgagtg gagaaggacg cgagtcatcc acaacgatgg cttttgtccg catactcaat
     2101 accctggtca aggacaagaa catcggtaag cgagtggtac ccatcgttgc cgacgaatcg
     2161 cgtacgttcg gcatggaagg tatgtttcgc caattgggca tatggtcctc aactggccag
     2221 ctctataccc ctcaagacgc tgaccaattg atgtattaca aggaggacaa gaacggccag
     2281 attctgcagg agggaatcaa cgaggctggg gcaatggcct cctggatggc cgcgtcaacc
     2341 tcgtacagca cccatggcgt gcagatggtg ccgttctaca ttttctattc aatgtttggc
     2401 tttcagcgcg tcggtgacct tgcgtgggcg gccggggata tgcggtgccg gggctttctg
     2461 ctgggaggaa ctgccggacg tactaccctg aatggcgaag gcctccagca tgaggatgga
     2521 cacagccatc ttgccgcttc aaccattccc aactgcatat cctacgatcc gaccttcgcc
     2581 tatgagcttg ctgtcatcat ccatgatggc ctgcgccgca tgaatgagca gcaggaagat
     2641 gtcttctatt acatcactgt gatgaacgag aactattcgc atcccgagat gcctgcaggt
     2701 gcggaagatg gcatattgaa aggcatgtac ctgtttcgcg aaagcaaaac cgcggaggag
     2761 gcgggcctgc gcgtgcaact gctcggttcg ggtactatcc tgcgggaagt cattgcagcc
     2821 ggggatatac tggaaaagga attcggagtc gtggccgata tctggagcgt gaccagcttc
     2881 aacgaggtgc ggcgggaagc actggatgtt gctcgatgga acatgctaca tccggcgagc
     2941 gagccaagaa tctcgcatgt cacggcctgc ctgaaggacc gccaggggcc agtggtcgca
     3001 gccacggatt atatgaaaat tttcgcggat cagattcgag cgttcgtgcc ggggagatac
     3061 aaggtgctgg gtacagatgg tttcggccgt tccgacacac gggagcagct gcggcttttt
     3121 tttgaggtta atcgttatta catcactatt gccgctctcg aagcgcttgc agaagacggc
     3181 aagatgccaa ttgaaaaagt gacgcaagca atagagaagt ttggcatcaa tcccgataag
     3241 ccgaacccgg caacggtttg aagcggatgt gagaggaaca ggtgggcgcg caccgggacg
     3301 gccaagcggc cgagtgcagg ttgtgctaaa tacgaacaaa gggaaaag
//
LOCUS       DASSHJ010000045         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_952000, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000045 DASSHJ010000000
VERSION     DASSHJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952000"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..888)
                     /locus_tag="VFI43_01995"
     CDS             complement(124..888)
                     /locus_tag="VFI43_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET7060930.1"
                     /translation="MNTRYFLISCEHGGNRIPSAYRRFFVGFSGLLRSHRGYDMGALR
                     MARDFAVMLHAPLFASTTSRLVVDLNRSIGNPRLYSEATRDAPRAIREEILECCYFPY
                     RNGVEDDIAEAIRQGHRVVHISSHSFTPELNGEIRNADIGLLYDPGRRLEQDFCNRWR
                     ASIRTRQPTLKVRRNYPYRGTSDGFAAYLRRRFSPDVYTGIELEVNQKHVFEGHRPWA
                     AFRRLLLETLREVISASKYVARDKSRYSSKGAAARP"
     gene            complement(916..2199)
                     /locus_tag="VFI43_02000"
     CDS             complement(916..2199)
                     /locus_tag="VFI43_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET7060931.1"
                     /translation="MSAPLPAFSGYGIELEYAIVDRENLSIMAIADEFLHKLSGSYNS
                     AVSVGEVGWSNELVEHQIELKNIKPHAAIESLLPAFQAEIQRADQILESMGAKLMPAG
                     MHPWMNPLVETNVWKGDNSEIYNAYDRIFGCRSHGWANLQSMHLNLPFGDDIQFERLH
                     AAIRILLPILPALAASSPIADGVYSGFLDFRMKNYCDHQMKFPITMGRVIPDTVTSPA
                     AYTDEILAQMYREVAGSDPEGILQYEWLNARGVIPRFDRNALEIRVIDSQECLQADLA
                     IAAAVTGIVHAFYDEVYATLEEQQAIGTDALAEILQSCIRDGELAIVDNAAYLQLMGF
                     PSRHCEARELWRNLTVMMHQSQVERSPNLEKAVDTILKQGPLARRILRTLGPVWQEAQ
                     LRAVYDELCTCLEEGRMFLGLKQSPERQAGSLSRS"
     gene            complement(2196..2927)
                     /locus_tag="VFI43_02005"
     CDS             complement(2196..2927)
                     /locus_tag="VFI43_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060932.1"
                     /translation="MNQMLRNTLYLPVKTLRQPDETTCGPTCLHAIYGYWGKTEPLGG
                     IIMRTRKLESGVGTFAVFLGCDALRNGFGATIYTYNLTVFDPTWFTTSVDIAERLRKQ
                     RDIKQDSRLRHATEGYLEFLRLGGRLRLTDLSQSLVHGLLRRNLPILTGLSSTFLYRT
                     AREYGPDDTPDDIRGFPAGHFVVIMGYNRDERSLLVADPYGPSQEYWSNIDRVLGAVL
                     LGVVTQDANLLVIHSRRSNGIGKKA"
     gene            complement(3123..>3729)
                     /locus_tag="VFI43_02010"
     CDS             complement(3123..>3729)
                     /locus_tag="VFI43_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7060933.1"
                     /translation="ADERLSQREPVLIITHGLPGSGKTTFAQAALEKLQLIRIRSDVE
                     RKRLFGLSKLWDSGTHPESIYSADATSLTYQRLHELSENLLRSGVGVIVDAAFLQEDE
                     RESFRRLANKMNVPFAIASLQATPAILPIRIVQRRESGNDASEADLSVLEKLRSHQQA
                     LTSDERVRAVEFSNEGNGFSDDAAAWRRLGNLISPLGPPDI"
BASE COUNT          829 a         1017 c         1037 g          846 t
ORIGIN      
        1 catgcactgt cgatgacttc gttcagatct tccatccctg cgaggtaaca aactggaagt
       61 gcctcggctc ctggagtgcg aaagttgcat atccgccgag catcgggctc attttattac
      121 cggctaaggt cgtgccgcag cgcctttact tgagtaccga gacttgtcgc gcgccacata
      181 tttgcttgct gaaatgactt cccggagcgt ttcaagcaac agcctgcgaa atgcagccca
      241 aggtctgtga ccttcaaata catgcttctg attgacctcc agctcgattc cagtgtaaac
      301 gtcgggggaa aagcgccggc ggaggtaagc tgcaaagcca tcggaggtgc cccggtaggg
      361 gtaattacgc cgcactttaa gggttggttg ccgagttctg atacttgctc gccaccggtt
      421 gcagaagtct tgctcaagcc gccggccggg atcgtagagg agcccgatgt ccgcgttgcg
      481 aatttcgcca ttgagttcag gcgtgaagct gtgcgaggaa atatggacga ccctgtgtcc
      541 ctgccggatt gcttccgcaa tatcgtcttc cactccatta cgatatggga agtagcagca
      601 ttccagaatt tcctcgcgga tggcgcgcgg ggcatcgcgg gttgcttctg aataaagccg
      661 cggattaccg atggagcggt tgagatccac gactagccgg ctggtagtgg aagcgaacaa
      721 cggcgcatgg agcatgaccg cgaaatccct ggccatcctt aacgcgccca tgtcataacc
      781 acggtggctg cgtagcaggc cgctgaagcc cacaaagaat cgacgataag cggaagggat
      841 ccggttgccg ccatgctcgc agctaatcag gaagtatctt gtattcactt ttttcttcct
      901 ctcggttaag attcattacg atcgtgacaa gcttccggcc tgtcgttcag gtgactgttt
      961 caagcccaga aacatccgtc cttcctccag gcaagtgcat agctcgtcat aaacagcgcg
     1021 aagctgcgct tcctgccaaa cggggccaag ggtgcgcagg attcggcgcg ccaatggccc
     1081 ctgtttaagg atggtatcga ctgctttttc caggttcggc gatcgttcaa cttggctctg
     1141 gtgcatcatt acagtcaggt tccgccatag ctcgcgcgcc tcgcagtgcc gggaggggaa
     1201 acccatgagc tggagatacg cggcgttatc gacgatggcc agctcgccgt cccgaatgca
     1261 ggactgcagg atttccgcaa gagcgtcggt tccgatcgcc tgctgctcct cgagggtggc
     1321 atatacctcg tcatagaatg catgaacaat gcctgttaca gccgcggcga tcgcaagatc
     1381 cgcctgcagg cactcctgcg agtcgatgac acggatttcg agggcattgc ggtcgaagcg
     1441 gggaatgacg ccccgggcgt tgagccattc atattgcaga atcccttccg ggtcggatcc
     1501 cgcgacttca cggtacattt gcgccagaat ttcgtcggta taagccgcgg gactggtaac
     1561 cgtatcggga atcacccttc ccatcgtaat gggaaacttc atctggtggt cgcagtagtt
     1621 cttcatgcga aaatcgagaa aacccgagta gacgccgtcg gcgattggcg aacttgccgc
     1681 gagcgcggga aggatgggca acaagatccg gatcgcagca tgcaagcgct caaactggat
     1741 atcgtcgcca aagggcaggt tgagatgcat gctctgcaga ttcgcccatc cgtgagacct
     1801 gcagccgaag atccggtcat aggcgttata aatctcgctg ttatcgccct tccatacgtt
     1861 tgtttcgacc agcggattca tccatgggtg cattccggcg ggcatgagct tcgcccccat
     1921 ggattcgaga atctggtctg cccgttggat ctccgcctgg aaagcaggca aaagagattc
     1981 gatggctgca tgcggcttta tatttttcag ctcgatctgg tgctccacca gttcgttcga
     2041 ccatcctact tcaccgactg aaactgccga gttatagctt ccggacagct tgtggaggaa
     2101 ctcatcggca atggccatga tggaaaggtt ctcccggtcc acaattgcat actccagctc
     2161 gatgccatag cctgagaatg ctggtaatgg cgcgctcatg cttttttgcc tatgccattt
     2221 gaccggcgcg aatgtatgac gagcaaattg gcgtcctgcg tgacaacccc aagcaggaca
     2281 gcccccagca cccggtcgat gttgctccag tattcctgtg aaggcccata tggatcagcc
     2341 accagcaacg agcgctcgtc acggttgtat cccatgatga cgacaaagtg gccggccggg
     2401 aaaccgcgta tatcatccgg ggtatcatcc ggtccatatt cacgggcggt gcgatagaga
     2461 aaggtggaac tcaacccggt cagtatgggg agattgcgcc ggaggaggcc gtgaaccaaa
     2521 gattgcgaga ggtcagtcaa ccgaagccgc ccacccaggc gcaggaattc gagatacccc
     2581 tcggttgcgt gccgcagccg ggaatcttgc ttgatatccc tctgcttcct tagccgctcc
     2641 gcgatatcca cgctggtagt gaaccaggta ggatcaaaaa cagtgaggtt gtaggtgtat
     2701 atggttgcgc caaaaccatt ccgcaaggca tcgcaaccca ggaagacggc aaatgtgccc
     2761 acacccgatt ccagcttccg cgtgcgcatg ataatgcccc ccagcggctc ggtttttccc
     2821 caataaccgt atatcgcatg gaggcaggtc ggtccgcagg ttgtctcatc aggttgtcgt
     2881 agtgtcttga caggcaagta aagcgtgttc ctgagcattt ggttcatttg gcagacagct
     2941 ggaaatggag taaccccagc ctgagccgca tttgttcggt agtattatta ttaaggttag
     3001 caggttgaag tgaattcgct tgccagcagc cgcttacgaa gtcatccccc agttacaaca
     3061 gccagaagtt aaacaactat ctttaaaaga cgctttcgtt gaaaaacggt tcctcaagac
     3121 ttttatatgt cggggggtcc gagggggctt atcaagttac ctaatctccg ccatgcggcc
     3181 gcgtcatcag aaaacccgtt tccttcgttg ctgaactcaa ccgcccgcac gcgttcatcg
     3241 gaggttagcg cttgctggtg ggaacgcagt ttttccagta ctgaaagatc ggcctcggaa
     3301 gcatcatttc cactctcccg gcgttgaaca atacggatgg gcaggattgc cggggtggct
     3361 tgcagcgacg caattgcaaa cggaacattc attttattgg caagccggcg aaagctttca
     3421 cgctcgtcct cctggagaaa ggcggcgtcg acaatcacgc ccacaccgga gcgcaggagg
     3481 ttttcggata gttcatgcag gcgctggtag gtgagcgatg ttgcatcagc actatagatg
     3541 ctttcaggat gagtcccgct atcccacagc ttggataatc caaacaggcg cttgcgctca
     3601 acatcggagc ggatgcgtat taattgcaac ttttcgagcg cggcttgagc aaaggtcgtt
     3661 ttaccggacc caggtaaacc atgtgtgatg atcagcacag gctcgcgctg tgacaaccgc
     3721 tcgtctgcg
//
LOCUS       DASSHJ010000046         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_840365, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000046 DASSHJ010000000
VERSION     DASSHJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840365"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFI43_02015"
     CDS             complement(<1..734)
                     /locus_tag="VFI43_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7060934.1"
                     /translation="MSNPAKIDTLLEAKWVIPVEPARVVLHDHAVAIDRGTIQAIVPR
                     AEAQIRFAPNERVVLGNHAVIPGFFNLHTHAAMSLMRGIADDLPLMEWLNERIWPAEA
                     KHVDPGFVFDGTRLACAEMLKGGITSFNDMYFFPEATVRAVLESGMRATIGMIAIDFP
                     SAYASDVSDYLAKGLALRDEFKSHPLLSFCFAPHAPYTVSNRSFASILAFAEQLDLPI
                     HIHLHETGDEILNSLQAHGVRPLERL"
     gene            complement(808..1485)
                     /locus_tag="VFI43_02020"
     CDS             complement(808..1485)
                     /locus_tag="VFI43_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7060935.1"
                     /translation="MKNMVKKKLLLGAAILPLLFSGAAMADEQPEGYASDGRGDVATD
                     SSGDCWRTGYWTPAMATYQCDPDLLPKPVKQVEAPAPAPAPEPAPVPAPVTEPEKLTL
                     SADELFDFDKAVLRPEGKQALDDLVSKLEGYKYDTIVAVGYADRLGSDAYNKKLSMRR
                     AEAVKTYLVQQKGIPADRVFTDGKGEANPVTGDTCKGNKKTKALISCLQPDRRVEVEV
                     AATRTPQ"
     gene            1805..4348
                     /gene="gyrA"
                     /locus_tag="VFI43_02025"
     CDS             1805..4348
                     /gene="gyrA"
                     /locus_tag="VFI43_02025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7060936.1"
                     /translation="MDQFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELSNDWNRPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYML
                     VDGQGNFGSVDGDNAAAMRYTEIRMSRISHELLADLDKETVDFGPNYDGSEKEPLILP
                     TKIPNLLINGSSGIAVGMATNIPPHNLNEVVNACLALLKNPEIGIDELIEIIPAPDFP
                     TAGIIYGVSGVKEGYRTGRGRVVMRARTHFEDMEKGSRQSIIVDELPYQVNKANLLIR
                     IGELVRDKKIEGISDLRDESDKSGMRVVIELRRGEVPEVILNNLYKETQMQDTFGMNM
                     VALLDGQPRLLNLKQILSAFLRHRREVVTRRTVFELKKSRERGHLLEGLAVALSNVDE
                     VIALIKAAPTPAVAKQSLMGRSWRSQLVEEMLARAMMDAQAFRPEGLAPEFGLSKDGY
                     HLSDAQAQAILDLRLQRLTGLEQDKIVGEYREVMEKIADLLDILAKPERITAIITDEL
                     AAMKQQFGDKRRSEIVIDTQDLSMEDLIAPEDVVVTLSHTGYIKSQPLADYRAQKRGG
                     RGKQATGTKEDDFIDNLFIANTHDYILCFSSRGRVYWIKVYSVPQGGRASRGKPIVNL
                     VQLEQGEKINAILPIKAFDENRYIFMATAFGTVKKTPLSDFSRPRPSGIIAVGLDEDD
                     YLIGVALTEGKHDVMLFSDSGKAVRFDENDVRPMGRGARGVRGMKLGKGQKVISLLVA
                     ESDHVTILTATENGYGKRSPIDEYTRHGRGSQGMIAIKTNQRNGKVVAARLVHDDDEI
                     MLITTGGVLIRTRVNEVREMSRVTQGVILINLDPGEKLAGLERVVETDE"
BASE COUNT         1083 a         1172 c         1234 g          955 t
ORIGIN      
        1 tgcaggcgtt cgagcgggcg gacgccgtgt gcttgcaggc tgttgagaat ttcatcgccc
       61 gtctcatgta gatgaatatg gatgggcagg tcaagttgct cggcaaacgc gaggatgctg
      121 gcgaacgacc tgttgctgac agtgtagggt gcatgtggag cgaagcaaaa agacagcaac
      181 gggtgagact tgaattcatc ccgcaaagcc aaacccttcg ccaggtagtc tgacacgtcg
      241 ctggcatacg cgctgggaaa atcaatggcg atcatgccta tggttgcgcg catgccggat
      301 tcaagcacgg cgcgaaccgt tgcttcaggg aaaaaataca tgtcattgaa gctggtaatg
      361 ccgcctttca gcatttcggc acaagccagt cgcgtcccat caaaaacaaa gccggggtcg
      421 acatgctttg cttctgcagg ccagatgcgt tcattcagcc attccatcag tgggagatcg
      481 tccgcaattc ccctcatcaa gctcatggct gcgtgagtat gcaaattgaa aaaacccggt
      541 atgacagcgt gattaccgag cacaactcgt tcattcggcg caaatcggat ctgcgcttcc
      601 gctcgtggaa cgattgcctg gatcgtgccc cggtctatgg caacagcatg atcatgcaat
      661 accaccctgg cgggttcgac ggggattacc cactttgctt ccagcagagt gtcgatcttc
      721 gcaggattgc tcatattcca aagcttatca aaaaaaaacc ctgccgactg gcagggtttt
      781 ttaatttctt actttaatgg agatttacta ctggggtgtc ctcgtagctg ccacctcaac
      841 ttcaacacgg cggtcgggct gcaagcatga gatcagcgct ttggtcttct tgttgccctt
      901 gcaggtatcg cctgttactg gattcgcttc acccttgccg tcggtaaata cacgatcagc
      961 aggtatgcct ttctgttgga ccaggtaggt tttgaccgct tcggcacgac gcatggaaag
     1021 cttcttgttg taggcatccg agcccaggcg gtctgcatag ccgacggcga cgatcgtgtc
     1081 gtatttgtag ccttcgagtt tcgatacgag gtcatccaaa gcttgcttgc cttcgggcct
     1141 cagcacggcc ttgtcgaaat caaacagctc atccgcagac agcgtgagtt tttcaggctc
     1201 ggttacagga gcaggaacag gagcaggttc gggagcagga gcaggagcag gcgcttccac
     1261 ttgttttacg ggcttgggaa gcaggtcagg atcacattgg tatgttgcca tggcaggcgt
     1321 ccagtagccc gtgcgccagc aatcccccga agaatctgta gctacgtcac cacggccatc
     1381 gcttgcatac ccttctggtt gttcatccgc cattgctgct cccgaaaaca gcagtggcag
     1441 aatggctgct ccaagcaaga gttttttctt taccatattt ttcatgctat gccccttttt
     1501 cagaaaaact tcaattcaaa ttaaccatcc cttagtccta caaattcagc aacagagcca
     1561 ctaacaaagc cacttattgt aatggcaatt attacacatc ttgatttgat tatgtattta
     1621 taaacctgct cttgtcaaat ggtggtgttg cattcggcat gaaaatcgga atatgggcgt
     1681 gacgttgttg ccgccggcat gataaaatta cgggttttta cacgctctca tccgtgccca
     1741 gccgggcctc aaaccgggac aggaccttcg caataagcct ttgtcccgga ttagaaaatc
     1801 atcaatggat caattcgcga aagaaactct tcccatcagc ctcgaagagg agatgcgccg
     1861 ctcttatctc gactacgcaa tgagcgtaat tgtaggacgg gcgttgccgg acgtgcgcga
     1921 tggcttgaaa cccgtgcacc gcagggtcct cttcgccatg cacgaactct ccaacgactg
     1981 gaaccgccct tacaagaaat ccgcccgcat cgttggcgat gtcatcggta aatatcatcc
     2041 ccatggggat accgcggtgt acgacaccat agtccggatg gcgcagaatt tttcgctgcg
     2101 ctacatgctc gtggacggac agggcaattt cggatcggtg gatggcgata atgcagcggc
     2161 aatgcgctac acagaaatcc gcatgtcgcg catctcgcat gagctgctcg cagacttgga
     2221 caaggaaacg gtcgacttcg gtccgaacta tgacggatcg gaaaaagagc cgctgattct
     2281 acccaccaag atacccaacc tgctgatcaa cggctcgtcc ggcatagctg tcgggatggc
     2341 aaccaatatt cccccccata atctgaatga agtggtaaac gcctgcctgg cccttcttaa
     2401 aaacccggag atcggcatcg atgaactcat cgagatcatc cctgcaccgg atttcccgac
     2461 cgcgggaatc atttacggcg tttctggagt aaaagaggga taccggacgg gacgaggccg
     2521 ggtcgtcatg cgcgcccgga cgcactttga ggacatggaa aaaggcagcc gccagagcat
     2581 catcgtcgac gaactgccct accaggtgaa caaggcgaat ctcctgatac gtatcggcga
     2641 actggtgcgt gacaagaaga tcgagggcat ttcagatctg cgcgacgaat cggacaagtc
     2701 gggaatgcgc gtggtcatcg aactgcggcg aggcgaagtg cccgaagtga tcctgaacaa
     2761 tctgtacaag gaaacacaga tgcaggatac cttcgggatg aacatggtag ccctgctgga
     2821 cggacagcct cgccttctga acctgaagca gatactgagc gcattccttc gtcatagacg
     2881 cgaagtcgtc acgcggcgaa ccgtcttcga gctgaagaaa tcccgcgagc gcggccacct
     2941 gcttgagggc ttggctgtag cactctcaaa cgtggacgaa gtcatagcgc tcatcaaggc
     3001 ggcgccaact cccgctgtgg ccaagcaaag cttgatgggg aggtcctggc gctcgcaact
     3061 cgtggaggaa atgctcgccc gagccatgat ggatgcacag gcgtttcgtc cagaggggtt
     3121 ggcgcccgag ttcggcttgt ccaaagacgg atatcacttg tccgatgcgc aagcgcaggc
     3181 gatcctggac ctgcgcctgc aacggctgac cgggctcgag caggacaaga ttgtcggtga
     3241 atatagagaa gtcatggaaa aaatcgccga tctgctcgat attttagcca agccagagcg
     3301 catcacggcc atcattaccg atgagcttgc tgcaatgaaa cagcaattcg gtgacaagcg
     3361 gcgcagcgag atcgtcatcg atacccagga cttgagcatg gaagacctga tcgcgccgga
     3421 ggacgtggtc gtgacgctat cgcacaccgg ctatatcaag tcccagcctc ttgcggatta
     3481 ccgggcgcaa aagcgcgggg gacgcgggaa gcaggcaacc ggcacgaaag aagatgactt
     3541 catcgacaac ctgttcatag ccaatacgca cgactacatc ctctgctttt ccagccgcgg
     3601 gcgcgtgtac tggataaagg tttactcggt tccgcaggga gggcgcgcat cgcgtggcaa
     3661 gccgattgtc aatctcgtcc aactggagca gggggagaaa ataaatgcca ttcttcccat
     3721 caaggcattc gacgagaacc ggtatatttt catggcgacg gccttcggca cggtgaaaaa
     3781 aacgccgcta tccgatttct cacgtccgcg gcctagcggg atcatcgcgg tgggcctcga
     3841 tgaggatgat tacctgatcg gcgttgccct gaccgagggc aagcatgacg tcatgctgtt
     3901 ctcggacagc ggaaaagcgg tgcgattcga tgaaaacgat gtccgcccaa tggggcgggg
     3961 agcgcgcggc gtgcgcggaa tgaaactggg caaaggccaa aaggttatct ctctgctcgt
     4021 tgcggaaagc gatcatgtga ccatcctgac ggcgaccgaa aatggttacg gaaagcgctc
     4081 tcctatcgac gaatacactc gtcatggccg gggcagccag gggatgattg cgatcaagac
     4141 caaccagcgc aatggcaagg ttgtggcggc aagactggtc catgatgatg acgaaatcat
     4201 gctgatcacc acgggcggcg tgctgatccg cacccgggtc aatgaagttc gcgaaatgag
     4261 ccgtgtaacg caaggcgtga ttttgatcaa tcttgacccg ggcgaaaaac ttgccggact
     4321 cgaaagagtg gttgagacgg acgaatagga caaaaagtgt ttttcgcaga tataagcatt
     4381 tctcagcggc gagtactacc atagtaggcg aaggtactaa gtaggcgaag ctactaaaga
     4441 gatt
//
LOCUS       DASSHJ010000047         7797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_185000, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000047 DASSHJ010000000
VERSION     DASSHJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185000"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="hprK"
                     /locus_tag="VFI43_02030"
     CDS             complement(<1..706)
                     /gene="hprK"
                     /locus_tag="VFI43_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET7060937.1"
                     /translation="MSRINVAQLFEERKQKLGLTWEAGRSGGNNWLDDDLLSHSTQGV
                     IGHLNFIHPNWIQVLSETEVNYLNQLDEPSLRENMQRLGQSRLFCIIVTGGAEIPASI
                     RELADSSDTPLLRSPQSSLEVIWLLRPYLGRALAPSTSRHGVLLDVLGLGIMITGESG
                     VGKSELALELVSRGHGLVADDVVELYRIAPETLEGSCPPMLRDFLEVRGLGMLNIRTI
                     FGETAVRRKKNMKLIVH"
     gene            complement(850..1185)
                     /gene="raiA"
                     /locus_tag="VFI43_02035"
     CDS             complement(850..1185)
                     /gene="raiA"
                     /locus_tag="VFI43_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7060938.1"
                     /translation="MNLTLTGHHVEITPAMRDYVTTKFTRITRHFDNVIDVSVILSVE
                     KLRKKAEANVHVRGKDIFVETDGADMYASIDNLVDKLDRQILRHKEKNIEHRNHHNGL
                     SKNPEADQI"
     gene            complement(1205..2662)
                     /locus_tag="VFI43_02040"
     CDS             complement(1205..2662)
                     /locus_tag="VFI43_02040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET7060939.1"
                     /translation="MKPTLQFKLSPHLTLTPQLQQSIRLLQLSTVELNQEIERITQEN
                     PLLELDEIADYRGAPEDSQPESISQDPVEIMDESTAQALAPDTEFDIGKDSVEEHDWR
                     DDGALRAGRDDDDERDFPQQAAEPPSLRDHLYLQLSLSQIPEHDRRIVGLLIDSLDDD
                     GYLAQDLEELASLLPPELEIDIDDLRIALEHLQHLDPPGVGARDLRECLIMQLRALPQ
                     ETPYREQALVLVSNHLESLAARDFCAIKKALRCADDCLRSIQQMIVRLNPRPGISFSS
                     TVARYIIPDVIVTKVSGGWAAHINPEAMPRLRVNRLYADILKRCHDDSARRLAGQLNE
                     AKWLIKNVQQRFSTILKVSSAVVERQQQFFEHGAVAMRPMVLREIADTLELHESTISR
                     VTTQKFMRTPRGIFELKYFFGSHVSTDSGGACSATAIRALIKQMINTENSRKPLSDSQ
                     ISEILGQQGIMVARRTVAKYRESIQIPPVNLRKSF"
     gene            complement(2711..3433)
                     /gene="lptB"
                     /locus_tag="VFI43_02045"
     CDS             complement(2711..3433)
                     /gene="lptB"
                     /locus_tag="VFI43_02045"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET7060940.1"
                     /translation="MSEFKADNLKKRYKSRTVVKDVSLAVNSGEVVGLLGPNGAGKTT
                     CFYMMVGLVPLDGGCIHLDERDLSQMPMHQRARLGLSYLPQEASIFRRLSVSDNIRAV
                     LELKNYTNGEMQEHLDDLLHDLHITHIRASQAASLSGGERRRVEIARALATAPRFILL
                     DEPFAGVDPIAVLDIQKIIHFLRDRDIGVLITDHNVRETLGICDRAYIINDGTVLANG
                     TPNEIIYNENVRKVYLGEHFRL"
     gene            complement(3461..4009)
                     /gene="lptA"
                     /locus_tag="VFI43_02050"
     CDS             complement(3461..4009)
                     /gene="lptA"
                     /locus_tag="VFI43_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HET7060941.1"
                     /translation="MCIRQTFIVLLTGMFSSAPVFAEKADRDKPVHLEADRATVQDSN
                     KLATFTGNVVLTQGTLTIRADKMTVKEDANGFQHATAYGNLASFRQKRDGKDEYVEGW
                     SERMEYDGKADKVQLFKKAKLRRGQDEVHGDYISYDAINEFFQVIGGSANEPTAQVRP
                     DGRVRAVIQPKKKEPNPDAESQ"
     gene            complement(4035..4610)
                     /gene="lptC"
                     /locus_tag="VFI43_02055"
     CDS             complement(4035..4610)
                     /gene="lptC"
                     /locus_tag="VFI43_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET7060942.1"
                     /translation="MISRFISALPFILFALFVVGVPLWLGQSPRAPSETRETVPTRVP
                     DYLMENITAVQMGDDGVARQMLFAKRVIHYADDDSIYLEEPYFVETQPGKPAVQAKSD
                     QAEIVNDNKDIYLKGDVMLIRNGNKSRDETTLSTSLLHLIPADDIAKTDKPVVIRESN
                     AVIKAIGMEMNNRTGVTKLVSQVKVVHGKSR"
     gene            complement(4607..5146)
                     /gene="kdsC"
                     /locus_tag="VFI43_02060"
     CDS             complement(4607..5146)
                     /gene="kdsC"
                     /locus_tag="VFI43_02060"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HET7060943.1"
                     /translation="MAAEMMQNVLEKARKIRVVIFDVDGVFTDGSLYLTDGGEEIKAF
                     NSRDGHGIKMLKATGVKVALITGRESQCVNLKATDLGITTVYQGAKNKLPAFEALLRE
                     LELDASCCAYAGDDLIDVPVMLRCGFAITVPAAPAFVKKHADYVTRMEGGRGAVREIC
                     EVIMDAQDTLSAHLETYLK"
     gene            complement(5540..6484)
                     /gene="ispH"
                     /locus_tag="VFI43_02065"
                     /gene_synonym="lytB"
     CDS             complement(5540..6484)
                     /gene="ispH"
                     /locus_tag="VFI43_02065"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7060944.1"
                     /translation="MIKVLLANPRGFCAGVDRAIEIVERALSMHGAPIYVRHEVVHNR
                     FVVENLEKKGAVFVENLDEVPVGSILIFSAHGVSHAVRREAEAQQFKVFDATCPLVTK
                     VHIEVAKMREQGREIIMIGHAGHPEVEGTMGQAEGDGPAMYLVETEEDVENLKVNDPT
                     HLAYVTQTTLSVDDATRVIKALKKRFPKITGPKKDDICYATQNRQDAVKMMVKQCDLV
                     IVVGSPNSSNSNRLCEVARNAGVDAYMIDRPEHLQEAWLEGRDRIGITAGASAPEVLV
                     QEVIARLRQLGAGPKGREVVVEELSGVVESVVFPLPKN"
     gene            complement(6640..>7797)
                     /locus_tag="VFI43_02070"
     CDS             complement(6640..>7797)
                     /locus_tag="VFI43_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET7060945.1"
                     /translation="SGRPDRVTIFIGFAAISLTIKLTFKVSDTCPGACIPVKGRRQRR
                     RRAFELLSVLMLPLFADIAFAGAVDSKLPASPLTPRNQETHGLELVVPLKLVDDVGRA
                     IRLARPAGRIVSLSPSITELVFEAGAGAKLVGIARHSDYPESARSIPEVGDASNLDLE
                     RIVSLKPDLVIAWRSGNAFSEVQKLEKLGLIVFATEANRLEDIPRLLRNTGYLAGTSL
                     QAEASATRFEEALSEIRRDYANRSKLKVFQMVWHQPLMTINGNHLMSNIIEMCGGVNI
                     FASSPFLTPVISVEDLLEANPEVIISSVSREGAEIEVKARMSRFPGVKAIRHNHMYSI
                     HPDLLHRQTPRLIEGVKAVCARMENARSARADEASTRGGAQAGLERKVLLR"
BASE COUNT         1714 a         2200 c         1973 g         1910 t
ORIGIN      
        1 ggtgcacgat aagcttcatg tttttctttc gccgaacagc tgtctcacca aaaattgtac
       61 ggatgttcaa cattccaagt ccccggactt cgaggaaatc gcgaagcatc ggagggcaac
      121 tcccttccag ggtttccggc gctatccggt aaagttcgac cacatcatcg gcaaccagtc
      181 cgtgtccccg gctcaccagt tccagcgcaa gttcactttt accgacaccg ctttctcctg
      241 tgatcatgat gccaaggccg agcacatcca gcagtacgcc gtgtctgctg gtcgatggag
      301 caagcgcccg tcccagatag ggccgcaaca gccagatgac ctcgagactg gactgcggcg
      361 aacggagcag aggcgtatcg gaagaatctg ccagttcgcg tattgatgca ggtatctccg
      421 cgccccctgt cacgatgatg cagaacaatc tgctctgccc gagccgctgc atgttctcgc
      481 gcagggaagg ctcatccagt tggttgaggt aattgacttc ggtctcgctg agtacttgta
      541 tccagttcgg gtggatgaaa ttgagatgac cgattacgcc ctgggttgag tgggaaagca
      601 ggtcatcatc gagccagtta ttcccgccac ttctgcctgc ctcccatgtg agacccagct
      661 tttgtttcct ttcctcaaat agctgcgcaa cattgattcg ggacattgaa acgatcccct
      721 tcagatatgt aagaaaggga agagctgacg cattctatgc ccgacactct ttggtattat
      781 tggcagaatg aatcgcgctc tccgggcttt cgccgggttg ggggattaaa agcaggacag
      841 cttttgccgt cagatttgat ccgcttccgg gtttttagac aggccgttgt ggtgattccg
      901 gtgctcgata tttttttcct tgtgcctgag tatttgccga tcaagcttgt ccacaagatt
      961 atctatagag gcgtacatgt ctgcaccgtc ggtttcgaca aaaatgtcct tgccgcggac
     1021 atgcacgttg gcttcagcct ttttcctgag cttttccact gaaagaatca cactgacgtc
     1081 gatgacgttg tcaaaatgac gcgtaattct ggtgaacttc gtggttacat agtcccgcat
     1141 cgcgggcgtg atttccacat ggtgacccgt tagagtgaga ttcattcgcg gctccttggc
     1201 ttggttaaaa cgacttccgg agattaacag gaggaatctg tatggactcc cggtattttg
     1261 ccacagtacg gcgcgcaacc ataatgccct gctgacccag aatttcggaa atttggctgt
     1321 cactcagcgg tttccgtgaa ttttcggtat tgatcatctg cttgatcaag gcgcgaatcg
     1381 ccgtggccga gcacgcgccc ccgctatctg tcgagacatg gctaccgaag aaatacttca
     1441 gttcgaatat cccacgaggc gtgcgcatga acttctgcgt ggtgacgcgc gaaattgtgg
     1501 attcatgcag ctcaagggta tctgcaatct cccgcagcac catgggacgc attgcgaccg
     1561 cgccatgttc gaaaaattgt tgctgtcgct cgacgacagc ggaagaaacc ttgaggatgg
     1621 tgctgaaccg ctgttgaacg ttcttgatga gccatttggc ttcgttcaat tggccagcca
     1681 gacgccgggc ggagtcgtcg tggcaccgct tgagaatgtc ggcatacaag cggtttaccc
     1741 gcagacgagg cattgcctcg ggattaatat gggcggccca gcccccgctc acctttgtaa
     1801 cgatgacgtc agggatgatg taccgggcaa ccgtggagct gaatgaaatg cctggcctgg
     1861 gattgaggcg cacgatcatt tgctggatcg aacgtagaca atcgtcggca caacgcaacg
     1921 ctttttttat tgcacaaaaa tcccgcgcag cgagactttc aagatgattt gaaaccagaa
     1981 ccaaggcctg ttcccggtag ggcgtttcct ggggcaaggc cctgagctgc atgatcaggc
     2041 attccctcag gtcccgggcg ccaactccgg gaggatcaag atgctgcaaa tgttcgaggg
     2101 caatacgcag atcatcgatg tctatctcga gctcgggggg caagagactg gcaagctcct
     2161 cgaggtcctg cgccagataa ccatcatcgt cgagactatc gatgagtaag cccacgattc
     2221 ttctatcatg ctcgggaatc tggctaaggc tgagctgcaa ataaagatga tcccgcaagc
     2281 tcggcggttc ggccgcctgt tgaggaaaat cacgctcatc gtcatcatcc ctgcccgcgc
     2341 gcaacgcgcc gtcgtcccgc caatcgtgct cctcgaccga atctttaccg atatcgaact
     2401 cggtatccgg agcgagcgcc tgagcggtcg attcatccat gatctccacc ggatcctgcg
     2461 agatcgattc gggctggcta tcctcgggag cgccgcgata atcggcaatt tcgtcaagtt
     2521 ccagcaaggg attttcctgg gtaatccgct cgatttcctg attgagttcg accgtcgata
     2581 attgcagcaa ccggatcgat tgttgcaact gcggtgtaag tgtcaggtga ggcgataact
     2641 tgaactgtag agtcggtttc atagttcgga aaaacagcca gaattgctgg cctggattaa
     2701 aactccgttt tcacaatcga aaatgttctc cgagatacac ttttctcacg ttttcattat
     2761 aaattatttc gttcggcgtc ccgttagcca gcaccgtgcc gtcattgatg atgtacgcac
     2821 ggtcacatat gcccaacgtt tcgcgcacat tgtgatcagt gatcaggacg ccaatatccc
     2881 tgtctcggag gaaatggatg atcttttgta tgtctagaac agcgatcgga tcgacgccgg
     2941 cgaaaggctc atccagcagg atgaaccgcg gggctgtcgc aagggcgcgc gcgatctcca
     3001 cgcgccggcg ctccccgccc gacagactcg cggcctggct tgcgcgtatg tgggtaatgt
     3061 gcaagtcgtg cagcaaatca tcgaggtgct cctgcatctc tccattcgtg taatttttaa
     3121 gttccaggac tgctcgaata ttgtcggata ccgaaagacg tctaaaaatc gacgcttctt
     3181 gaggcaggta acttaacccg aggcgggcac gctgatgcat tggcatctgg ctcaagtcac
     3241 gctcgtccag atgtatgcag cctccatcca gtggaaccaa acccaccatc atgtaaaagc
     3301 acgtcgtttt tccggcgccg ttagggccaa ggagaccgac aacctcgccg ctgttcacgg
     3361 ccagcgagac atccttcacc acggttcggg acttataacg ctttttcagg ttgtcagcct
     3421 tgaactcgct cattttgact tataaattgg tggttcgaat ctattggctc tctgcatctg
     3481 gattcggctc tttcttttta ggctggatta ccgcccgcac ccggccgtcc gggcggacct
     3541 gggccgtcgg ttcgttcgcg ctccccccga tcacctggaa gaattcgtta attgcgtcgt
     3601 aggaaatata atcgccgtgc acttcatcct gcccccgcct caatttcgcc tttttaaaaa
     3661 gctgcacctt gtccgccttc ccatcgtatt ccatgcgttc gctccagcct tcgacatatt
     3721 catcctttcc gtcgcgcttc tggcggaaac tcgcaagatt tccatatgct gtcgcgtgct
     3781 gaaatccgtt ggcatcctct ttcacagtca tcttatccgc gcgaatcgtc aaggtgccct
     3841 gcgtcaggac gacatttccg gtaaaggtgg cgagcttgtt ggaatcctgc actgtcgccc
     3901 ggtcggcctc cagatgaacc ggcttatccc ggtccgcctt ctccgcgaac actggagcac
     3961 tcgaaaacat gccagtgagc agaacgatga aagtttgcct gatacacatg cgcggcgagg
     4021 aaaacgagag agatttaacg tgacttccca tgcacgacct tcacctgcga caccagttta
     4081 gtgacacccg tgcgattatt catttccatg ccaatcgcct tgataactgc attcgattcc
     4141 ctgatcacga cgggtttatc cgtttttgcg atgtcatcag cgggaatgag atgcagcaag
     4201 ctggtggaca gcgtggtctc atcgcgactc ttgtttccat tccggatgag catgacgtcg
     4261 cccttcaaat aaatatcctt gttatcgttc acgatttctg cctggtctga tttcgcttgc
     4321 acagccggct tgccgggctg tgtctccacg aaatagggct cctccaggta aatcgaatca
     4381 tcatccgcgt aatggatgac tcttttggcg aagagcatct gacgagcaac tccgtcgtcc
     4441 cccatttgaa cggccgtgat attctccatc aggtagtcag gaacacgcgt cgggactgtc
     4501 tcacgcgttt cagaaggcgc tctgggcgac tggcccagcc acaaggggac ccccactaca
     4561 aacaacgcga ataaaatgaa gggaagcgcg gaaataaacc ggctgatcat ttgagataag
     4621 tttccaggtg ggcgctcagc gtatcctggg catccatgat cacttcacaa atttcacgga
     4681 ctgcaccccg gccgccttcc attctggtga cgtagtccgc atgttttttt acgaaagcag
     4741 gggcggcagg caccgtgatg gcgaatccgc accgcaacat gacgggaacg tcaataagat
     4801 cgtcgcccgc ataggcgcag caggatgcat ccagttctag ttcccgcagc aatgcctcaa
     4861 acgccggcag cttgttcttc gccccctggt aaacagtcgt gatgcccaga tccgttgcct
     4921 tcaggtttac gcactgcgat tccctgcctg tgatcaaggc aactttgact cccgtcgctt
     4981 tgagcatttt tatgccgtgg ccatcacggc tgttgaatgc cttgatctcc tccccgccgt
     5041 cagtgaggta gaggctcccg tccgtgaaaa ctccatccac atcgaaaatg acgacgcgaa
     5101 tctttctagc cttttccagc acgttctgca tcatctccgc tgccataaaa ctgagacatc
     5161 cggcaacgga gaaagcgctc tccttttcct gcgagcggaa agtatcttca tttttcgtcc
     5221 ctgacattgc atatggaaga gcccgatttt tcagatgaac ttataaaagc gcggggaatg
     5281 ccgagccgcc tgccgccggg gccaggacag cctatgctaa ttgtcgattg accttctacg
     5341 gacagctttc ttgaaacctt caggacgatt aacgccattt ttacagcttt cttaaagcct
     5401 ggcggggaga tgaaaccagc cgagatgcat taaaggttgg tttcgttcgg acacgaatac
     5461 ttgaaagagc gttttatagt cgcctttttg ccacccgatt ttttgtactc aaattttagg
     5521 tacccagtct ttttggcact caattttttg gcaggggaaa caccaccgac tcaacaacgc
     5581 cgctaagttc ctccaccacc acttcccgtc cttttggtcc cgctccaagc tgcctgagcc
     5641 gtgcgatcac ttcctgcacc agcacttcgg gagccgatgc tccggccgta attccaattc
     5701 gatcccttcc ttccagccat gcttcctgca agtgctccgg acgatcaatc atataagcat
     5761 ccaccccggc atttctcgcc acttcgcaaa gacggttgga gttcgaacta ttgggtgaac
     5821 cgaccacgat gacgagatcg cattgtttca ccatcatttt gaccgcgtcc tgtcggtttt
     5881 gcgtggcata gcagatgtcg tcctttttag gacccgttat tttcgggaag cgcttcttca
     5941 acgccttgat cacgcgggtc gcgtcatcca cggacaatgt agtctgagtg acataggcca
     6001 ggtgggtggg gtcgttgacc ttcagatttt cgacgtcttc ctctgtttcc accagataca
     6061 tcgccgggcc gtcaccttcc gcctgcccca tcgtgccttc cacttcggga tgtccggcat
     6121 gacctatcat gatgatctct cttccctgtt cacgcatctt ggccacttcg atatgcacct
     6181 tggtaacaag cgggcaagtg gcgtcaaata ccttgaattg ctgcgcttcc gcttcacgcc
     6241 tcactgcgtg ggatactccg tgcgcgctga agatcaggat gcttcccact ggaacctcat
     6301 cgaggttttc gacaaatacc gccccttttt tctccaggtt ctccactaca aagcggttat
     6361 gtaccacctc atgccgtacg taaattggcg caccgtgcat gctaagcgcc cgctcgacaa
     6421 tttcaattgc ccgatccacg cctgcacaga aacccctggg attggcaagc agtaccttga
     6481 tcattggtcg ttctccataa gactgttgtt taattgagga catcgtttca ctccaagcgc
     6541 ttgaatatcg gttccggaca ataccgttcg ttgcggacgg atgcaaaaaa atgggcaata
     6601 aagggggaca gagacaaaac gcgcatttta gagcatccgt tatctcaaga gaacttttct
     6661 ctcgaggccg gcttgagcgc ccccccgcgt tgacgcttca tccgcacgcg ccgacctcgc
     6721 gttttccatc ctggcgcaga ctgccttgac gccttcgatc aggcggggcg tctggcgatg
     6781 cagcagatcg gggtggatag aatacatatg gttatgacga attgccttca cgcccggaaa
     6841 gcggctcatt cgagccttta cctcaatctc ggcgccttca cgcgatacgc tactgatgat
     6901 gacttcaggg ttggcctcga gcaagtcttc cacggaaatt acgggggtta agaaaggtga
     6961 agatgcgaat atgttgaccc ctccgcacat ctcaatgatg ttgctcatga gatgattccc
     7021 atttatagtc atgagcggct ggtgccagac catctggaac actttcagtt tcgaccgatt
     7081 ggcgtagtcc cgccggattt ccgataatgc ttcctcaaac ctggtcgccg acgcttccgc
     7141 ttgcagggat gttcctgcca gataacccgt attgcgcaat agccggggaa tatcttccag
     7201 gcggtttgcc tcggtagcaa agacaatcaa tccaagcttt tccagcttct gcacctccga
     7261 gaaggcattg ccgctacgcc aggcaatcac gagatcgggc ttcaagctca cgatgcgctc
     7321 caggtccaga ttcgacgcat caccgacttc aggaatcgac ctggcgctct cggggtagtc
     7381 actgtgacgg gcaatgccta ccagcttggc tcccgctccg gcttcgaata ccagctcggt
     7441 aatactggga gacagtgaaa cgatacgccc cgcgggccga gccaggcgaa tggcgcgccc
     7501 cacgtcgtca acgagcttca gcggcacgac caactccaag ccatgcgttt cttgatttcg
     7561 gggagtcaaa ggcgaagccg gaagtttgct gtccacggcg ccggcaaagg caatatccgc
     7621 gaaaagcggt aacatcaata cggacagcag ttcaaaagcg cggcgacgcc gttggcgcct
     7681 acctttaaca gggatacaag ctccagggca ggtatcggaa actttaaaag ttaatttaat
     7741 agttaaagaa atcgcggcga acccgatgaa gattgtgacc cgatcaggcc gcccgct
//
LOCUS       DASSHJ010000048         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1410973,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000048 DASSHJ010000000
VERSION     DASSHJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410973"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFI43_02075"
     CDS             <1..1107
                     /locus_tag="VFI43_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET7060946.1"
                     /translation="TWMAKILPEDEHLSNDGRVMEILSEHICQGWLEGYLLTGRHGLF
                     SCYEAFIHIVDSMFNQHAKWLKVTSKEIPWRRPIASLNYLLTSHVWRQDHNGFSHQDP
                     GFIDLVVNKSADVIRVYLPPDANTLLFITDKCLRSRNFINVVVAGKQPEPQWLDMDSA
                     IKHCTTGIGIWSWASNDQDGETDVVMACAGDVPTLETLAAVDLLHQHVPDLRIRVINV
                     VDLMTLQPRQEHPHGLSDRDFDVLFTTSKPVIFAYHGYPWLIHRLTYRRTNHENLHVR
                     GYKEEGTTTTPFDMTVLNDLDRFHLVMDVIDRVPKLGYKAAYLKQMMRDKLIDHKQYV
                     TRYGEDMPEIRNWRWSASNGKPADGRGGREAGEA"
     gene            1347..1970
                     /locus_tag="VFI43_02080"
     CDS             1347..1970
                     /locus_tag="VFI43_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060947.1"
                     /translation="MVIFIVNGIAIFASLSLNYLNALYWGPILPALYALVVAPHALIG
                     RPGIPRSAILKTLIAKWNNAEDLSGYIVKYWMAFAYPESSWKKRRNAVTLYVTSFFLC
                     ACYFLNELFVAGVSMFAAGYVLYQMSLRVDRPRLVYANRSFRESGSYPSGRAEWELAA
                     MSIIAFADLFPEDRALKESAGRILEDSDVKNLLAHHRYEYGASWVVV"
     gene            1993..4413
                     /gene="ppsA"
                     /locus_tag="VFI43_02085"
     CDS             1993..4413
                     /gene="ppsA"
                     /locus_tag="VFI43_02085"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HET7060948.1"
                     /translation="MGDSPCVIGFDKVGISDVNRVGGKNASLGEMITQLARAGVRVPG
                     GFATTAEAYRDFLAQAGLAQRIEDLLGKLDTEDIAALADAGAKIRAWIKETPLPSRLR
                     QDIASAHDALARKAGTPDFPMAVRSSATAEDLGDASFAGQQETFLNIRGLDNLLEAVR
                     NVFASLYNDRAISYRVHKGFTHSDVALSVGVQRMVRSDIGSSGVMFTLDTETGFDKVV
                     FITSSYGLGETVVQGAVNPDEFYIYKPALEQGKFPILRRNLGSKLIKMELTAEGIAGR
                     SVQTVDVDEAARRRFSITDAEAEELARQALIIERHYDRPMDIEWGKDGVDGKLYILQA
                     RPETVQSRSRMDVFQRYRLKEKSETLVSGRAIGQKISGGPVRLIPDAKEMSRVQEGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIVARELGIPAVVGCGDATERLSENDE
                     VTVSCAEGDIGNIYRGKLKVEVTDLSLEKMPKPPVKIAMNVANPQLAFAFQRIPNDGI
                     GLARLEFIIAQMIGIHPKAALNYSNLSPELKREIDDRAAGYPDPVSFYVEKLAEGIGM
                     LGAAFFPKPVIVRMSDFKSNEYSSLIGGEQYEPKEENPMLGFRGASRYVADSFRDCFE
                     LECRALKKAREAIGLTNLEIMIPFVRTVAEARHVVESLEKNGLKRGENGLRLIMMCEI
                     PSNALLADQFLQYFDGFSIGSNDLTQLTLGLDRDSSLVAQSFDERDPAVKALLSAAIR
                     SCRKAGKYVGICGQGPSDHPDLALWLMDEGIESLSLNPDSVVETWLYLAKEVKKKESK
                     KESPRTVH"
     gene            complement(4580..>4883)
                     /locus_tag="VFI43_02090"
     CDS             complement(4580..>4883)
                     /locus_tag="VFI43_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7060949.1"
                     /translation="IATTLGARIVCEQALAYSKTHHIKSVVLPDKIAVSASLQFLQDH
                     GIVVEPACGVALAAVYEKVAALSGYKTVLVIVCGGVSSTADHLVELARLECDVAKC"
BASE COUNT         1153 a         1300 c         1348 g         1082 t
ORIGIN      
        1 acatggatgg caaaaatcct gcccgaggac gagcatctct cgaatgatgg acgagtgatg
       61 gaaattctct ctgaacacat atgccagggg tggctggaag gctatctgct gactggacgg
      121 catgggttgt tttcatgcta cgaggctttt atccatatcg tcgattccat gttcaatcag
      181 catgcgaaat ggctgaaggt gaccagcaag gagattccgt ggcgcagacc catcgcctcg
      241 ctcaattacc tgctcacttc gcatgtatgg cggcaggacc acaacggttt ctctcatcag
      301 gatcccgggt tcatcgacct cgtcgtcaac aagagtgcgg atgtgatccg cgtttacctg
      361 ccgcccgatg ccaacacgct actttttatt actgacaagt gcctgcgttc gcgcaatttt
      421 atcaatgtcg tcgtggcagg aaagcaaccc gagccccagt ggctcgatat ggattccgca
      481 ataaagcact gcacgacggg aatcggtatc tggagttggg ccagcaatga tcaggatgga
      541 gaaaccgacg ttgtaatggc gtgtgcaggc gatgtcccca cccttgaaac gcttgctgcg
      601 gtcgacctgt tgcaccagca cgtgcctgat ctcagaataa gggtaatcaa cgtggtggac
      661 ctgatgacgt tgcagcctcg gcaggagcac ccgcacgggt tatcggatcg cgatttcgac
      721 gtcttgttca cgacctccaa accggtcatt tttgcgtatc acggttatcc ctggcttatt
      781 caccgcctca cttaccggcg aaccaaccac gaaaacctgc acgtgagagg atacaaggag
      841 gaaggaacga ctaccactcc tttcgatatg acggtactta atgatcttga tcgcttccac
      901 ctcgtcatgg atgtgatcga ccgtgtcccc aagctcggct acaaagcggc ttatctcaag
      961 cagatgatgc gcgacaagct gatcgaccac aagcaatatg tcacccggta cggggaggat
     1021 atgcctgaaa taaggaactg gcgctggtct gcatcaaatg gaaaaccggc ggatgggaga
     1081 ggaggccggg aggcgggcga ggcctgatcc atattggttt ataacctttg ccacaacaac
     1141 ctccgccaca tgtgcctgtt ttcccgtctt gccattcgct ttcaattgca tcaaacatcg
     1201 acatggttga tcattacgtt tgttgatcat taggtttggg atcaggctca aaccgcgcaa
     1261 agcagacctg cccgccgtca agttctggcc gggattgccg gatttataag gagcaaagca
     1321 ggacaacttt gcgatcatgg aggtgggtgg tgatattcat cgtcaatgga atagcgatat
     1381 ttgcttcgct gtcgctcaat tatttgaacg ccctttattg gggccccatc ctgccagccc
     1441 tgtatgctct cgtcgttgcg cctcatgcgt tgatcggcag gcctggcatt ccccgctcgg
     1501 cgatactgaa aacgcttatt gcaaaatgga acaatgccga ggatctgtcc ggatacatcg
     1561 tcaagtactg gatggctttt gcatatccgg aaagctcctg gaaaaagcgg cgcaacgccg
     1621 tgacacttta tgtcacatca ttcttcctgt gcgcctgcta tttcctcaac gaactgttcg
     1681 tggcaggggt ttccatgttc gcagcgggat acgtgctcta ccagatgagc ctcagggtgg
     1741 atagaccgag actggtttac gcgaaccgat cattccggga gagcggtagc tatccatctg
     1801 gaagggcgga atgggaactc gccgcgatgt caatcattgc attcgccgac ctgttccccg
     1861 aagatcgggc tttgaaggaa tcggcaggcc ggatcctgga ggatagcgac gtaaaaaacc
     1921 tgctggcgca tcatcgctat gaatacggcg cgagctgggt ggttgtctga caaaaaacgg
     1981 ggagcaatcg gaatgggtga ttcaccgtgc gtaattggct tcgacaaagt gggtatatcc
     2041 gacgtgaacc gggtcggggg gaagaacgcg tcccttgggg aaatgatcac ccaactggct
     2101 cgagcaggcg tacgcgtgcc aggtggtttt gcgaccactg cggaagctta tagggatttt
     2161 ctcgcccagg cgggccttgc ccaacgtatc gaggacctgc ttggaaagct ggatacagaa
     2221 gacattgccg cgctggctga tgcgggcgcc aagattcgcg catggataaa ggaaacgccc
     2281 ctgccatcca gactccggca ggacatagca tccgcacatg acgcattggc tcgtaaggct
     2341 ggaactcccg attttcccat ggcagttcgc tcctcagcaa cagcggaaga tctcggtgac
     2401 gcttcttttg caggacagca ggaaaccttc ctgaacatcc gcggactgga caatctgctt
     2461 gaggcagtca ggaatgtttt tgcctccctt tacaatgacc gcgccatctc ttaccgcgta
     2521 cataaaggtt ttacccattc cgatgtcgcg ctgtcggtcg gggtgcagcg gatggtgcgc
     2581 agtgacatcg gttcatctgg cgtcatgttc accttggata ccgaaaccgg cttcgacaag
     2641 gttgttttta tcacgtcttc ctatggtctc ggtgaaacag tggttcaagg cgcagtgaac
     2701 cctgatgagt tttatatcta caaaccggca ctggagcagg gcaaattccc tatcctgaga
     2761 agaaacctcg gctccaagct catcaagatg gagcttacgg cggaagggat cgctgggcgg
     2821 tcggtccaga cagtggacgt ggatgaggcc gcacgaaggc gcttttcgat taccgatgca
     2881 gaggcggagg aactggcgcg gcaggctctg atcatcgagc ggcactacga caggccgatg
     2941 gatatcgagt ggggcaagga cggcgtggat ggaaagctct acatcctgca ggcaaggccc
     3001 gaaaccgtgc aatcccgctc gaggatggat gtctttcagc gttaccggtt gaaggaaaaa
     3061 tcggaaacac tggtttccgg gcgcgccatc ggtcagaaaa tcagcggcgg cccggtccgc
     3121 ctcatacccg atgcaaagga aatgtctcgc gttcaggaag gggatgtatt ggttaccgac
     3181 atgacggacc ccgactggga accggtgatg aaacgggctt ccgccattgt caccaaccgt
     3241 ggcgggcgca cctgtcacgc ggccatcgtg gcgcgcgaac tgggcatccc ggcggtggtc
     3301 ggttgcggag atgccactga gcggctatcg gagaacgatg aggtaaccgt gtcctgtgcc
     3361 gaaggtgata tcggcaatat ttaccgaggc aagctcaagg tcgaagtgac cgacctctcg
     3421 ctggaaaaga tgccgaaacc tcctgtcaag atcgcaatga atgtcgcaaa tccgcaactg
     3481 gcctttgctt tccagcgcat acccaacgat ggcatcggcc tggcaagact ggagttcatc
     3541 attgcgcaga tgatcggcat ccatcctaag gccgctctca actattcgaa tctttccccg
     3601 gaattgaagc gggaaataga tgaccgggca gcaggatacc ccgatcccgt cagcttttac
     3661 gttgaaaaac tcgctgaagg aatcgggatg ctgggtgcag cgttttttcc caagccagtg
     3721 atcgtgcgga tgtcggattt caaatccaac gaatactcga gcctcattgg tggggagcag
     3781 tacgaaccca aagaagagaa tcccatgctc ggcttccgtg gagcctccag gtatgttgcg
     3841 gacagcttcc gcgattgctt cgaactggaa tgccgcgcgt tgaaaaaagc ccgcgaggcc
     3901 atcggactga ccaatctcga aatcatgatc ccctttgttc ggacggtagc tgaagcgagg
     3961 cacgtcgtcg aatcattgga gaagaatggc ctcaagcggg gcgaaaacgg gctgcggctt
     4021 atcatgatgt gcgagattcc gtcaaacgcc ttgctggcgg accaatttct ccaatacttc
     4081 gatggatttt cgattggctc aaacgatctc acccagctca cgcttggact ggatcgcgac
     4141 tccagcctgg ttgcccaatc cttcgatgag cgtgatccag cagtcaaagc cctgttgagt
     4201 gcggcgatca ggtcatgccg caaagccgga aaatatgtcg gcatttgtgg tcaggggcct
     4261 tccgatcatc ccgatctcgc cctctggctg atggatgagg ggatcgaaag tctctcactc
     4321 aatccggatt cggtagtgga aacgtggcta taccttgcaa aggaagtaaa aaagaaagag
     4381 tcgaagaaag agtcccccag gacggtgcat tagccctggc tcgcaggggt aaggttttgg
     4441 ccgtaggccc ttccctgcac ccctgcccaa gtggcgaacg cggggggacg tgaacatcca
     4501 ctgccccatg cgcctctgcg ctattcccaa ccatttttgc caacgttaga aacgatacca
     4561 ccgcaagcat actgaatact caacattttg ctacgtcaca ttccagacgt gccaactcga
     4621 ctaaatgatc agcggtggaa ctaactcccc cgcacacgat aaccaatacc gttttatatc
     4681 ctgataacgc cgcaactttt tcgtaaactg cagcaagcgc aacgccgcac gcaggctcaa
     4741 ccacaattcc atggtcttgc aaaaactgca aggaagcaga aacggctatc ttgtccggca
     4801 acacaacgct tttgatatgg tgagttttag agtacgccaa tgcttgttca caaactatgc
     4861 gcgctcctaa cgtcgtcgcg ata
//
LOCUS       DASSHJ010000049         6905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_950419, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000049 DASSHJ010000000
VERSION     DASSHJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950419"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(520..1206)
                     /locus_tag="VFI43_02095"
     CDS             complement(520..1206)
                     /locus_tag="VFI43_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HET7060950.1"
                     /translation="MQIVTALLVWTLVFSSPLASATDALTSEDMSGGVKQLLDQSANA
                     AVDKLGVPNGFLDNPKVRITLPASLQKAEGVMRGLGMNKPVDNLVGSMNHAAEAAAAE
                     AKPVLLEVVQKMSLEDARKILTDGGDAATQYFRDTTSDALAQKLLPIVKNAAEQAGVL
                     KKYNEFAGKGAKLGLISEKDANVEKYITRKTMDGIYLMMAEEERAIRSNPMGQVKELQ
                     NVFNFLKKSR"
     gene            1331..2103
                     /locus_tag="VFI43_02100"
     CDS             join(1331..1646,1646..2103)
                     /locus_tag="VFI43_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558226.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET7060951.1"
                     /translation="MEITEIQYQQIESCLPRQRGNVSLSNLQVLNAFLYVAEHGCKWR
                     GLPKRFGNWHTIYTRMNRWSKSGVLDRLFEQLQHAQIIRIKIEAVSLDSTIVKVHPDG
                     TGAFKKNGLQSIGKSRGGWTTKIHLVAANARTAITFALSPGQAHDAPEGRALLNRLGP
                     TSYPLYLVMDRAYEGDETRQLAFDLGYVPVVPPKSSRINAWEYDREMYKRRNEIERLF
                     RRLKGFRRIFSRFEKLDVIFIGFIHFALIVEAFRSVNTL"
     gene            complement(2100..3068)
                     /gene="lipA"
                     /locus_tag="VFI43_02105"
     CDS             complement(2100..3068)
                     /gene="lipA"
                     /locus_tag="VFI43_02105"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET7060952.1"
                     /translation="MKTEKTEIPQTETRQKGAAKTARNPVKIQPQGLAERLPKPSWIR
                     ARSSNSKEFYEVKRILREQKLHTVCEEASCPNIGECFGKGTATFMILGDLCTRRCPFC
                     DVAHGKPFAPDPDEPLHLARSIAKMRLKYVVITSVDRDDLRDGGAQHFADCIREVRAH
                     SPLTKIEILVPDFRGRLDVALEKLFECPPDVLNHNLETVPRLYRQCRPGADYVHSLKL
                     LKDFKARFPSIPTKSGLMLGLGETDEEILQVMRDLRAHEVEMLTIGQYLQPSGGHLPV
                     TRYVPPEGFKEFERAAAEMGFSNAACGPMVRSSYHADQQAHEAGIT"
     gene            complement(3107..3838)
                     /gene="lipB"
                     /locus_tag="VFI43_02110"
     CDS             complement(3107..3838)
                     /gene="lipB"
                     /locus_tag="VFI43_02110"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET7060953.1"
                     /translation="MQSAQRGNGTLTHPRLKQHPASSETQGDIQVRYQGIVEYLSAWQ
                     AMKEFTACRTASTPDEIWLLQHLPVYTQGVACRPEHLLYEPGIPVIRTDRGGQITYHG
                     PGQVIAYLLLDMRRLKLGVRELVRKMEGAVIDLLDEYSVHAEGRLDAPGVYVRGAKIA
                     ALGLKIKNGCCYHGVALNVEMDLAPFEAINPCGYPGLRVTQTSNIGISDGLEPLSIKL
                     FEKLHARLRATSESPAGTALGATQE"
     gene            complement(3805..4119)
                     /locus_tag="VFI43_02115"
     CDS             complement(3805..4119)
                     /locus_tag="VFI43_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HET7060954.1"
                     /translation="MDSSDRPSLIEYPCEFPIKIIGRVASSDSASPAELEQARQEFTR
                     SILEIVKFHAPDFNDVNLEARISKKNTYLSLTCTIPATSREQLDSLYRDLCNQPNVVM
                     VL"
     gene            complement(4128..5000)
                     /locus_tag="VFI43_02120"
     CDS             complement(4128..5000)
                     /locus_tag="VFI43_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HET7060955.1"
                     /translation="MIYLNGKFMPIEEARIPVLDRGFIFGDGVYEVIPVYSHKPFRLP
                     EHLERLQHSLDGIRLRNPHSDSEWSALVQQLIDLNESDDQSLYLHVTRGVARRDHAFP
                     KDVPPTVFMMSNPLLAPPQELLETGVAAITAADNRWLRCDIKSISLLPNVLLRQTAID
                     SGAVETVLFRDGFMTEGAASNIFVVKNETLLAPPKNNLMLPGITYDVVLELVDAHQIR
                     HEVRAVSEYEVRTADEILLTSSTREIMPITLLDGKPVGNGRPGLVFEVLYDLYQQYKL
                     TVMRGGEAKLRVEG"
     gene            complement(5027..6166)
                     /locus_tag="VFI43_02125"
     CDS             complement(5027..6166)
                     /locus_tag="VFI43_02125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7060956.1"
                     /translation="MKRLPLLLFCLFVLPATAQQPQLTRPPQDFSIAAKSYALIDLQS
                     GQTLVGQNVHDRVEPASLTKLMTAYVVFGALYQKRISLTQAVPVSDQARRAPGSRMFI
                     DPSKPVTVDELIRGVIVQSGNDASIALSEAVAGSESAFTQLMNKEAGRLGMKNTRFVN
                     STGLPDPDHYSTAHDLGLLAAAIVRTFPEYYPLYSLREYTYNKITQTNRNRLLWLDPN
                     VDGIKTGFTEGAGYCLISSAKRGERRLVSVVMGTISDSARTIESQRLLNYGFQYYDTV
                     RLYAKEREVTAIQLWKGTQDKLRTGFAQDVYFSLPRSQADKLKAKMEFKQPLIAPVSA
                     GQKVGTVKFMLDGKLMGERPLVALEAVSSGNLLGRAVDSVRLLFN"
     gene            complement(6328..>6905)
                     /locus_tag="VFI43_02130"
     CDS             complement(6328..>6905)
                     /locus_tag="VFI43_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7060957.1"
                     /translation="RVQHALRAFTPRDQKAVRSAAATLRANPRIDTAEAITELSVGEA
                     LISLLDENGRPAMVERAFILPPASRIGPISDAERSAIIASSRLRNRYEQAIDRESAYE
                     IINNGASTGNEAGRKVASRSTGRTDNGSQEDLKHVLSEVLLGKTGPRGGHQPGILDSV
                     AKSAARSIGSQVGREIFRGVLGSIFGGSRRR"
BASE COUNT         1541 a         1883 c         1810 g         1671 t
ORIGIN      
        1 catttcccgg aatgagcatg cctcgtttgc tagtttcgag atgatttgtt cgagcatatt
       61 gatgtgctca tctcgaattt ctatcatact tccaacatgc ctctcaaatg ggaagcttac
      121 gaggaaactt tatttccgtt gtttaacata acctgcaatt gacaattccc cagtgaatgc
      181 aaccaatgaa gggtaaggat gtaacagtct taattgggat tcttggatga tactgcttgc
      241 aacaaggcaa tggaaatgtc ggccacatct agagaaatca gttgagccga cttaaccaga
      301 catttcacta gaaaccagtg gacgaagctt gctggctttc tgagcgggga aaccaagtgt
      361 aaagcgttat ggaaatcgtt cgctccaatg cgagatcgcc ctgtcactga aagggcccgg
      421 tcggtgactt cacaggcgac tcgggcagtg ttcagcaatg aatacggaat taagttttgc
      481 aacccgaaag ccattcaaac cgatgtgcgc attcccttgt tacctgctct ttttaaggaa
      541 attaaaaaca ttctgtagct ctttgacctg ccccattgga ttgctgcgaa tggcgcgctc
      601 ttcctccgcc atcatcaaat aaattccatc catcgttttt cgggtgatgt acttttcgac
      661 gttcgcgtct ttttccgaaa tcaatcccag ctttgctcct ttacctgcaa attcgttata
      721 tttctttaac acgcctgcct gttccgcagc attttttaca atcggcaaca atttctgagc
      781 cagtgcatcc gacgttgtgt cacgaaagta ttgcgtcgca gcatcgccgc cgtcggtaag
      841 gattttccgc gcgtcttcca gcgacatttt ctgcaccacc tccaatagca cgggtttagc
      901 ttctgcggca gccgcctctg ccgcatggtt catgctgcca accaggttat ccacgggctt
      961 attcatgcct aaaccgcgca tcacgccctc cgccttttgc agcgaagcgg gcagcgtgat
     1021 tctaaccttg gggttgtcaa gaaatccgtt gggaacgccc agcttgtcta ctgctgcatt
     1081 ggcgctttga tccagtagct gcttcactcc gcccgacata tcctcgcttg taagggcatc
     1141 ggtagctgaa gcaagggggg atgagaaaac aagagtccag acaagtaagg cagtgacgat
     1201 ttgcattccc aattcctccg gacttacgtt aaccttcagt ctcgaacaac gtctggacgg
     1261 aactagggcg tgttaacact aatggttgag cttctgatat acttcggata taaaaaccac
     1321 ggggagccgt atggaaatta ccgaaattca atatcagcag atcgaaagct gcttgcctcg
     1381 gcagcgaggc aacgtgagtc tttcgaatct tcaagttctc aatgccttcc tgtatgtagc
     1441 cgaacacggc tgcaagtggc gcggtctgcc aaaacgcttt ggcaattggc acacgatcta
     1501 tacgcgaatg aaccgttggt ccaagagcgg cgtgctggat cgattgttcg agcaattgca
     1561 gcatgcccag atcatccgta tcaagatcga ggctgtgtct ctggacagca cgatagtaaa
     1621 ggttcatccc gatggcacgg gggctttaaa aaaaacggcc tccaatcgat cggcaagtcg
     1681 cgcggaggat ggactaccaa gattcatctg gttgccgcga atgctcgaac ggctataacc
     1741 tttgcattgt ccccaggtca ggcacacgat gcccctgaag gccgggctct gctcaatcgg
     1801 cttggcccaa caagctatcc tctttatctt gtgatggatc gcgcctatga aggcgacgaa
     1861 actcgccaac tcgcgttcga tcttggttat gtgcctgtcg tcccaccaaa gagcagtcga
     1921 atcaatgcct gggaatacga tcgagagatg tacaagcgcc gcaatgaaat cgagcgcctg
     1981 tttcgcaggc tcaagggctt tcggcgaata ttctcgcgtt tcgaaaaact cgatgtcatt
     2041 ttcatcggat ttattcactt cgctctgatc gtcgaagcat tccgtagtgt taacacgctc
     2101 taggtaatgc ctgcttcgtg cgcctgctgg tcggcatggt agctggaacg taccatcggc
     2161 ccgcaagccg cattactgaa tcccatctcg gccgctgcac gttcaaattc cttgaaccct
     2221 tccggcggca cataccgtgt gaccggcaaa tggccgccac tcggctggag atattggccg
     2281 atcgtaagca tctccacctc gtgggcgcgt agatcgcgca tcacctggag gatctcttca
     2341 tccgtttctc ccagccccag catcaagccc gactttgtgg ggatcgatgg gaagcgtgct
     2401 ttaaaatcct tcagcagctt gagtgagtgt acatagtccg cgccgggacg acattggcga
     2461 taaaggcggg gaacggtttc caggttatga ttcaggacgt caggcgggca ctcgaataat
     2521 ttctccagcg caacgtccag ccttcccctg aaatccggaa cgagaatctc gatctttgtg
     2581 agcggagaat gagcccgcac ctcacggatg cagtccgcga aatgctgggc gcctccgtcg
     2641 cgcaggtcgt cgcggtcaac actggttatg acaacatact tgagcctcat tttcgcgatg
     2701 gagcgcgcca gatgcagagg ctcatcgggg tcaggggcaa atggcttgcc atgggcgacg
     2761 tcgcagaagg ggcagcgccg ggtacacaaa tccccaagga tcatgaaggt ggcggtgcct
     2821 ttgccgaaac actccccaat gttcgggcac gaagcttctt cgcaaaccgt gtgcaatttt
     2881 tgctcacgca gaatgcgctt gacctcgtaa aattccttgc tgttggatga gcgggcacga
     2941 atccaggacg gtttgggcaa ccgttccgca agcccctgcg gctggatctt gacggggttg
     3001 cgcgctgtct tggcagcacc tttctgccgg gtctctgttt gagggatttc tgtcttttct
     3061 gtcttcatag taaatacttt ttacccatac cttctatcca tgccttctat tcctgcgtgg
     3121 cacccaaggc agtacctgca ggggactcgc tggtggctcg aagtcttgca tgcagttttt
     3181 caaaaagctt gatgctcaat ggctccaatc cgtcactaat tccaatgttg cttgtctggg
     3241 tgactctcaa gcccggatag ccgcacggat tgattgcctc gaagggcgcc agatccattt
     3301 ccacgttcag ggccaccccg tgatagcagc aaccgttctt aattttcaga cccagcgcag
     3361 cgatttttgc gccacggaca taaacacctg gtgcatccag gcgtccttcc gcatgaacac
     3421 tgtattcgtc cagcaagtcg atcaccgcgc cttccatttt tctgaccagc tcccgcacgc
     3481 cgagtttaag gcggcgcatg tcgagaagca gataggcaat gacctgcccc gggccatgat
     3541 aagtgatttg cccgcccctg tccgtcctga ttaccgggat cccgggttcg taaagcaaat
     3601 gttcgggcct gcaagccacc ccttgcgtgt ataccggcag gtgctgcaat agccatatct
     3661 cgtccggcgt gcttgctgta cggcaagccg taaattcttt catggcttgc caggcggaaa
     3721 gatattcaac aatcccctgg tatcttacct ggatgtctcc ctgcgtctct gaagaagcgg
     3781 ggtgctgctt caagcgtgga tgggtcagag taccattacc acgttgggct gattgcatag
     3841 gtcccgatac aaggaatcaa gttgttcacg cgaagtcgcg ggaatcgtgc aggtcagact
     3901 gagataagtg ttttttttgc ttatccttgc ttccagattt acgtcgttga aatcaggagc
     3961 gtgaaacttg acgatttcca atatgctgcg tgtgaattcc tggcgggcct gctccagttc
     4021 tgccggactt gccgaatcgg atgaggcgac ccgcccgatg atcttgatgg gaaattcgca
     4081 cgggtactcg ataagggatg gccgatcgga tgaatccata gttggggtta gccttcaacc
     4141 ctgagcttgg cctctccgcc gcgcataacg gtgagcttat attgctgata gaggtcatat
     4201 aacacttcga aaaccagccc gggtctgcca ttgcccaccg gcttgccatc gagcagcgtg
     4261 atgggcatga tttccctggt tgaggaagtc agcagaatct cgtcggcggt tcggacttcg
     4321 tactcggata cagcgcgcac ttcatgccgt atctggtgtg cgtcgaccaa ttcaagcacg
     4381 acatcgtagg taatgccggg cagcatcagg ttatttttcg gcggagcaag caatgtttcg
     4441 ttctttacca cgaaaatatt gcttgcagct ccttctgtca taaaaccgtc gcgaaacaat
     4501 acggtctcta cggcgcctga atcaatggcc gtctggcgca gcaatacgtt gggcagcagc
     4561 gaaatcgact tgatgtcgca gcgcagccat cggttatccg cagccgtaat agcggcaacc
     4621 cctgtttcca gcaattcctg cggcggcgca agcagtggat tgctcatcat gaatacagtg
     4681 gggggaacgt ctttaggaaa agcatgatca cgccttgcaa ccccccgggt gacatgaaga
     4741 taaagagact gatcgtcact ctcgttcaaa tcgatcaatt gctgcaccag tgcactccac
     4801 tcactgtctg aatgaggatt tctcaggcgg attccatcca ggctgtgctg caggcgctcc
     4861 aggtgctcag gcaagcggaa cggcttgtgc gaatagacag gaatgacttc gtacacgccg
     4921 tcgccgaaaa tgaatcccct gtccagtacg ggaattcgcg cttcttcgat tggcatgaat
     4981 ttgccgttga gataaatcat tatgccttgt caatcctcat caattcctag ttgaataata
     5041 ggcgaacgct gtccactgcc cggcccaaaa ggtttccgct gctcacagcc tccagcgcga
     5101 ccagggggcg ctcacccata agcttgccat ccagcatgaa ttttactgtg ccgacttttt
     5161 gcccggcact gacgggtgcg atcaacggct gcttgaattc catttttgcc ttcaacttgt
     5221 ccgcctgaga tcggggaagc gaaaaataga catcctgagc gaacccggtc ctgagcttgt
     5281 cctgagttcc tttccatagc tgaatagcgg ttacctcgcg ctccttcgcg tataagcgaa
     5341 cggtatcata gtactggaag ccgtagttca gaagccgctg gctttcgata gttcgcgcgc
     5401 tgtccgagat tgtgcccatc acgacggaaa ccaaccgacg ctcaccgcgt ttggcggacg
     5461 aaatgaggca gtagcccgct ccttcggtaa atcccgtctt gatcccgtct acgttgggat
     5521 cgagccacag caagcggttg cggttcgtct gggtgatctt gttgtaggtg tattcgcgca
     5581 atgaatacag gggataatat tcgggaaaag tccggacaat ggcggcggcg agcagaccaa
     5641 gatcatgggc ggtgctgtaa tgatccggat ctgggagccc tgtcgagttg acgaaccgcg
     5701 tatttttcat ccccagacgt ccagcctcct tgttcatgag ctgagtgaaa gcgctttccg
     5761 aacccgcaac ggcttcagac agtgctatgg aggcatcgtt gcccgactga acgatcaccc
     5821 cgcgtatcag ttcatcaacg gttacaggct tgctcggatc aatgaacatg cgcgagcctg
     5881 gagcacgccg agcttggtcg gacacgggca ccgcctgggt gagcgagatg cgcttctgat
     5941 agagagcacc gaaaaccacg tacgcggtca tgagtttggt gagcgacgca ggctcaactc
     6001 gatcgtgaac gttttgccca accagagtct gtccgctctg gagatcgatg agagcgtagg
     6061 atttggcagc gatggaaaaa tcttgcggcg gtctggtcag ttggggctgc tgggctgttg
     6121 caggcaaaac gaacaagcag aacaacagaa gaggtaaacg tttcattgac aactaaacgt
     6181 tcgtcaaaac gatattatac cctagccacc tgaccggcgt gacctgtggc tccggcaggg
     6241 gcagccagaa ttccttcgcc aacccgtcga ccttttgaat gcaaatctgg accaatacct
     6301 gagccttcag tacgaggaac gcctccttca ccgccttcga cttcccccga agatactgcc
     6361 cagcacgccc cggaagatct cgcgtccaac ttgagagcca atggaacgtg ccgcgctttt
     6421 cgctaccgaa tccaggatgc ccggttggtg cccgccccgt ggccctgttt tcccgagcaa
     6481 aacttcgctc aggacatgct tcagatcctc ttgggagccg ttgtcggtcc tgcctgtcga
     6541 tcgggacgcc acttttcgcc cggcttcatt gccggtactc gcaccgttat taatgatctc
     6601 atacgccgat tcgcggtcaa tggcctgttc ataacggtta cgcaggcggg aagatgcgat
     6661 aatggctgag cgctcagcat cggaaatcgg cccgatccgg gatgcgggtg gcagaataaa
     6721 agcgcgctcc accattgccg gccggccatt ttcgtccagc agcgagatga gggcttcgcc
     6781 gaccgagagt tcggtaatag cttccgctgt atcaatccgg ggattggcac gaagggtggc
     6841 ggctgccgat ctcaccgctt tctggtcgcg cggggtaaat gcacgtagtg catgttgcac
     6901 tcgat
//
LOCUS       DASSHJ010000050         3832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1494889,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000050 DASSHJ010000000
VERSION     DASSHJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494889"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFI43_02135"
     CDS             <1..199
                     /locus_tag="VFI43_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060958.1"
                     /translation="LLEQYENKSYLYGRQAQDLQSHTSALIRDYEKSVKASEKLATLH
                     RQMAARLNQNHAANSHSASGL"
     gene            complement(557..1525)
                     /locus_tag="VFI43_02140"
     CDS             complement(557..1525)
                     /locus_tag="VFI43_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060959.1"
                     /translation="MSGNSDGVLPPPEALIRQIALETSEAVSEDFIVLAEAMRSRFGA
                     SLDAILLYGSCLRSREIGDGIADFYVVVSSYHKAYPQNYLRLMNDWLPPNVFYLEVER
                     DEKKFRAKYAVVSMGHFEEGTRHWFHPYLWARFAQPSRLLHARDDAIRRRIHESLAHA
                     VLKFLKSSLPMLGSSAVDAEAIWTNGLMQTYAAELRPERATRARQLVQLNLEDYTLLT
                     RNALPALDKVLEALPGDSYHPLADAHERRRASLRWRLRRWQGKILTVLRLAKAAFTFN
                     DSINYAAWKIERHTGVRVEVTPMLRRHPVLWGFKVSWKLLRRGVLR"
     gene            complement(1527..2522)
                     /locus_tag="VFI43_02145"
     CDS             complement(1527..2522)
                     /locus_tag="VFI43_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7060960.1"
                     /translation="MDRFSAGTARVGFLFNPLSGRIRKRRDVLRRMLDGIPGATRREV
                     TNASEINGSIDAFIRDDTDLLVIVGGDGTVQGALCHLFATQPLTNWPLLAIVPGGTTN
                     MTALDLGHSGSPEQILQQLKDSLLKRALPALLQRHVLCIEQTGAVKVYGMFFGVGLIA
                     RAVIFSRSPIKRLGITGEIYSGIITLGYLAGVLLGLRSGAWAPVQMSIMEGDDERHHG
                     SFAFLFASTLDRLLFGMRPYWGQEQEPLHITFVRQQQKHPLRSLMQLLSGGRNVLNDK
                     NGYHSCNKRVLELLIDDDYIVDGESYHAARERGPLRITAVGPVTFLAGSTSSVRS"
     gene            complement(2515..3732)
                     /locus_tag="VFI43_02150"
     CDS             complement(2515..3732)
                     /locus_tag="VFI43_02150"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7060961.1"
                     /translation="MTFAFISGDSETEIWGLSGRERLKRMLSSFQQCRLINEPAQMPE
                     HEPGLILRGDHVFDARVLSALMKAEQDCLLVSDTGQVIAMRTSDKGSYALAYLMDEGT
                     GQSFSTLPRYTVEDLNLLNVRQNLKKRDQPYVFPIFEENRGKLESELFARSYKGVTDL
                     VTKWLWPVPAYWAVRFCVGTGLRPNHVTLLSLLFAVLAGVAFWYGFYGFGLVMGWLMT
                     FLDTVDGKLARVTVTSSRAGDVLDHGLDIVHPPLWYIAWAVGLPEAPAWMPDMPVLIV
                     LILAGYIGGRLCEGAFQFWVASFDIFIWRPVDSFSRLITARRNPNLILLTLGWLAGRP
                     DVGLISVIVWHLISTVFLALRVAMGWLARRTNGPLRSWFEEILPGRDDGKLATRIFTP
                     VMVKVNDRKVADG"
BASE COUNT          917 a         1116 c          995 g          804 t
ORIGIN      
        1 gctgctggag caatatgaaa acaagagcta tctatatggc cggcaagctc aagatctcca
       61 atcgcatacc tccgcgctaa tccgtgatta cgagaaaagc gtgaaagcga gcgaaaaatt
      121 agcgaccttg catcgtcaaa tggctgccag gctcaatcaa aatcacgccg ccaactcgca
      181 ttccgcgagc gggttgtaac aatttcggag aaacggcctg agggtgcgtt cgcttgcatg
      241 ccttaggcct ggtcggaaag ctgagaggtc ccatctgttt gaaacgaaac ctccgttcaa
      301 tcaagtacaa ggccttcctc ctaagcgccg agttccactt ggctagcatg gaattgatgt
      361 gctgccgcgt cacggaggac gttcaaggcg gcggtctgga ttgccaaagc aatccggttc
      421 gctgctgcag ccctccgcga gcgcggcatc agtcccgccg tctcccatga ccttcactcg
      481 tcggcccctg ataccctgct tcgcttgccc acgagcctag tacgctgaga tatcgaagta
      541 gcggtggtaa cgcgcgtcaa cgcagcactc cccgccgcaa taacttccag gaaactttga
      601 aaccccacag aacgggatgc cggcgcagca tgggcgtcac ttccacacgc accccggtat
      661 gtcgctcgat tttccatgct gcgtaattga tgctgtcatt aaaggtgaat gccgccttgg
      721 cgaggcgaag caccgtgaga atttttccct gccaccggcg caatcgccag cgtagtgatg
      781 ctcggcgacg ctcgtgcgcg tccgccaagg gatggtaact atcccccggt aaagcttcga
      841 gcaccttgtc taatgcgggc agggcattgc gtgtaagaag cgtgtaatcc tccagattga
      901 gctgcacaag ctggcgcgca cgtgttgcgc gttcaggcct caactccgct gcataggtct
      961 gcatcagacc gttcgtccag attgcttccg catcgacagc ggatgagccc agcatgggga
     1021 gactggattt aaggaatttc aagacagcgt gggccaagga ttcgtggata cgtctgcgaa
     1081 tggcatcatc gcgggcatgc agcaatcgcg aaggctgtgc aaagcgcgcc catagatagg
     1141 gatgaaacca gtgccgcgtc ccttcttcga agtgccccat ggatacgacc gcatacttcg
     1201 cgcggaactt cttttcatcc cgttcgactt ccagataaaa cacattcggc ggcagccagt
     1261 cgttcatcaa acgcaagtaa ttttgaggat atgctttgtg atagctgctt acaacaacgt
     1321 aaaaatcggc gattccatca ccgatctcac gcgaacgaag gcatgagcca tagagaagaa
     1381 tagcatcgag cgaggcgcca aagcgcgacc tcatcgcttc ggccaggaca ataaagtctt
     1441 cggatacggc ttcggaagtt tccagggcta tttgccgaat caatgcttcc ggtggtggca
     1501 gcacgccatc tgaattgccg gacatatcag ctccgtacac tgcttgtgga ccccgccaga
     1561 aaggtgaccg ggccaactgc agtaatgcgc aggggccccc gctcacgcgc ggcgtgatag
     1621 gactctccat cgacaatata gtcatcgtcg atcaaaagct ccagcacacg cttattgcaa
     1681 ctgtgatagc cgtttttatc gttgaggacg ttcctgcccc ccgagagcaa ctgcatcagg
     1741 gaacgcaacg ggtgcttctg ctgttgacgt acaaaagtta tatgcaaagg ctcctgctcc
     1801 tgtccccagt atggccgcat tccaaacagg agtcgatcga gggtgcttgc gaacagaaat
     1861 gcaaatgacc cgtgatgacg ttcatcatct ccctccatga tcgacatctg aaccggcgcc
     1921 caggcgccgc tgcgcaatcc gagtagcacg cccgccaggt agccaagagt gataatcccg
     1981 gaataaattt cgccggtaat acccagccgt ttgatcgggc tccgggagaa tatcactgcc
     2041 cgcgcaataa gtcccacgcc gaaaaacatc ccgtaaacct ttaccgcgcc tgtctgctcg
     2101 atgcatagca cgtgccgctg caacagtgcg ggcaaggcgc gctttaacaa agaatccttc
     2161 aattgctgga gaatctgttc cgggctgccg ctatgcccga gatccagcgc agtcatattg
     2221 gtcgtacccc cgggtacgat ggccaggagt ggccagtttg tgagcggttg cgttgcgaac
     2281 aaatggcaca atgcgccctg caccgttcca tccccaccta ctattaccag caaatcggta
     2341 tcgtcacgga taaaggcatc aatcgatccg ttaatctcgg acgcattcgt aacttccctg
     2401 cgggtggcgc ccggtattcc gtcaagcatt cgtctcaaga catccctgcg cttgcggatg
     2461 cgtccgctga gggggttgaa gagaaatccg acgcgggccg ttccagcgct gaacctatcc
     2521 atccgcaact tttctgtcat ttaccttgac catcactggc gtaaaaatac gggtagcaag
     2581 cttcccatcg tctcggcctg gcagaatttc ttcaaaccat gagcgcagag gaccgtttgt
     2641 tcggcgtgcc aaccagccca tcgcaacccg caaggcaaga aaaacggttg agattaaatg
     2701 ccagacgatg acagatatca agccgacatc aggtctgcct gccagccagc cgagtgtaag
     2761 caggatcagg ttgggatttc gccgcgctgt gataaggcgg ctgaacgaat caacagggcg
     2821 ccagatgaag atatcgaacg acgcgaccca gaactggaat gcgccctcgc acaaccgccc
     2881 cccaatataa ccagcaagta tcagcacgat cagaaccggc atgtccggca tccatgccgg
     2941 ggcctcgggt aagcctactg cccaggcaat gtaccagagc ggtggatgta cgatgtcgag
     3001 cccgtgatcg agaacgtctc cggcccggct tgaagtaacc gttactcttg ccagttttcc
     3061 atccaccgtg tccaggaaag tcatcaacca tcccatgacc aggccgaacc catagaagcc
     3121 ataccagaaa gcgaccccag ccaacactgc gaataacagg ctcagcaatg tgacatgatt
     3181 cggtctcaag cctgtgccta cgcaaaaacg gactgcccag tatgcaggaa ctggccacaa
     3241 ccattttgta accaggtccg ttacgccctt gtaagaccgg gcaaacagct ctgattcaag
     3301 cttgccgcgg ttttcttcga agataggaaa gacgtagggc tgatctcttt tcttgaggtt
     3361 ttgccgaaca ttcagaaggt tcaaatcctc gaccgtgtag cggggcaggg ttgagaagga
     3421 ctgccccgtt ccttcatcca tcaagtacgc caaagcataa gaccccttat ccgaggtccg
     3481 catggcgatc acctggcccg tgtcgctgac gaggaggcag tcctgttccg ccttcatcaa
     3541 tgcggacagt acccttgcgt cgaaaacgtg atctccgcgc aagatgaggc cgggctcatg
     3601 ttccggcatt tgagcgggtt cgttgatcag gcggcattgt tgaaaagaac tcagcatgcg
     3661 cttgagccgc tcccgcccgc ttaaacccca gatttctgtt tcactgtcac cgcttataaa
     3721 tgcaaatgtc atcaagatat tacctgtacg gcctgtacag ccggtctgtc gatcaaatgg
     3781 cttaatccct cccgtcgctg cctgaaatgg gccgtctcaa acaggggtat ta
//
LOCUS       DASSHJ010000051         4357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1383091,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000051 DASSHJ010000000
VERSION     DASSHJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383091"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1461)
                     /gene="tilS"
                     /locus_tag="VFI43_02155"
     CDS             complement(58..1461)
                     /gene="tilS"
                     /locus_tag="VFI43_02155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7060962.1"
                     /translation="MSSRVENVLRQRVQSGDHLVVALSGGVDSVVLLNLLASFSSRIS
                     FSLSAIHVDHGISPNASRWAEFCSELCRAEEIPLKISKVRVLKQPGTSLEASARDQRY
                     FEFGTLEADYVVLAQHQDDQAETLLLQLLRGAGVKGLGSMPVVRDHSPGSAVLDTGAF
                     LSTGKEQRGPTRPKLLRPMLEVSRREIEDYAEEHRLSWISDESNIDLSFDRNFLRHQI
                     FPLLERRFPGYRQTLSRVSRHMAETSILLDELAEADGKGCAASGRLEIAALRKLSLRR
                     AKNLLRYSLVQQGAILPSTAKLDDLLRQLMSSRPDSKMHVIFGDTEIRCFRGELHVRK
                     ARNEKDRITGTPTPDWQLKWNGEEQLEVRELGGIVRFSRSEGVGINLQKLVESPVWVR
                     PRRGGERLRPHYNRPRRSLKNLLREAGLPHWERQRLPLIFSGEHLVCVPGIGVDSDYQ
                     AAPGEQGLVVSWHRDGG"
     gene            complement(1463..2431)
                     /locus_tag="VFI43_02160"
     CDS             complement(1463..2431)
                     /locus_tag="VFI43_02160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET7060963.1"
                     /translation="MKTTFLDFEQPVAELEAKIDELRFAQEDSAVDISSEITRLQKKS
                     QSLTQSIYTKLTPWQISQVARHPQRPYTLDYIQHLFTDFEELHGDRSFADDFAIIGGL
                     ARFNDQSVLVIGHQKGRDTKEKIHRNFGMPKPEGYRKALRLMRLAEKFSIPLLTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYVLSELRVPVICTIIGEGGSGGALALAVGDVVQMLQ
                     YATYSVISPEGCASILWKSADKAPEAAEIMGITAQRLASLGLVDKIINEPVGGAHRDY
                     EGVMQSVSKALQDSLGQLQQYSIEALLERRFERLMGYGKFKEAKVK"
     gene            2795..3916
                     /locus_tag="VFI43_02165"
     CDS             2795..3916
                     /locus_tag="VFI43_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7060964.1"
                     /translation="MTETLFTRANEVMAAAFCLLISANVCARDGIGETNGSRSPFNLS
                     GYAEAYYGVDFNDLDSNRKPSFFVSYTKNDKVSVNLGFVKGSYVTNNTRINLALAAGS
                     YVRANYAAEPEILKHIYEANIGIKLGGRSNLWVDLGVFPSHIGFESAIGKDNWTLTRG
                     IGADNTPYFETGAKIGYTSNNEKWFFSALILNGWQHIKPVPGNSIPAFGTQVTYKPSP
                     RIILNSSTFIGSDKPDSNRQMRYFHNFYGSLKLNDELAAMVGFDIGFEQKSKESSAMN
                     MWFNPEAMLRYTPTAKIALAFRVEYYDDRHGVMIPSGTPGGFKTWGFSTNFDYKLTNN
                     ILWRVEARTLRSKDKVFTRDDGSAADSMLFVTTSLAVNF"
     gene            4136..>4357
                     /locus_tag="VFI43_02170"
     CDS             4136..>4357
                     /locus_tag="VFI43_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7060965.1"
                     /translation="MISTANITMQFGARPLFENVSVKFGDGNRYGLIGANGSGKSTFM
                     KILGRDLEPTSGNVAVDAGERVGKLRQDQF"
BASE COUNT         1037 a         1188 c         1087 g         1045 t
ORIGIN      
        1 gcttaagccc caggtaggac gcttcttcga cgccattcgt ctgttgtgac ccttctccta
       61 tcctccatcc cggtgccagc tcaccaccaa cccctgctcg cccggcgctg cctggtagtc
      121 actatccacc ccgatacctg gcacacagac caaatgctct ccgctgaata tcagcggcaa
      181 tctttgccgt tcccaatggg gaagtcctgc ctcgcgtaaa agatttttca ggctgcggcg
      241 cggtctgtta tagtgaggac gaaggcgttc ccctccccgg cggggccgga cccaaacggg
      301 tgattccacc agcttttgca ggttgatgcc tacaccttca ctgcgtgaga accggacgat
      361 tccgccgagc tccctgacct cgagctgctc ttcgccattc catttcaact gccaatctgg
      421 agtcggtgta cctgttatcc gatccttttc gttccttgct ttgcgcacat gaagctcccc
      481 gcggaaacaa cgaatttcgg tgtctccgaa aatcacatgc atcttgctgt ccgggcgcga
      541 ggacatgagt tgccttagca gatcatcgag tttcgccgta ctgggcaaaa tggcgccttg
      601 ctgcacaagg ctgtagcgca acaggttttt ggcgcgccgc aggcttagtt tgcgcaatgc
      661 cgctatctcc agcctgccgg aagcagcgca tcctttgcca tcggcctcgg ccagttcgtc
      721 cagcaggatg gaagtctcgg ccatatgacg gctcacgcga gaaagggtct gccggtagcc
      781 cggaaagcgt ctttcgagca gtggaaaaat ctgatggcgc aggaaattac gatcgaacga
      841 aagatcgatg ttgctctcgt ccgaaatcca gctcaaacga tgttcctcgg cgtagtcctc
      901 gatctcgcgc cgtgaaacct ccagcatggg ccgcaataat tttgggcggg taggcccgcg
      961 ttgttccttg cctgtcgata agaatgcacc agtgtcaaga actgcgctgc ctggggagtg
     1021 atctctcacg accggcattg atcccaggcc ttttaccccg gcccctctca ataattgcaa
     1081 aagcagcgtt tcagcctggt catcctgatg ctgcgccaat acgacgtagt cggcttcaag
     1141 tgttccgaac tcgaaatagc gctggtcgcg agctgaagct tccagactcg tgcctggctg
     1201 cttaaggacc cgtaccttgg agatcttgag ggggatttct tcggcccggc ataactcact
     1261 acaaaactca gcccatctgc ttgcatttgg actgatgcca tggtcgacgt gaatagcgga
     1321 aagcgaaaag gatattcgcg atgaaaacga cgccagcaga ttcaatagta cgactgaatc
     1381 gactccaccg ctcaaagcca caaccagatg atcgcccgat tggacgcgtt ggcgcaggac
     1441 gttctctacc ctgctggaca aactatttga ctttagcttc cttgaacttg ccatacccca
     1501 tcagccgctc aaagcgcctt tccaggagcg cctcgatcga atactgctgc aactgcccaa
     1561 gcgagtcctg cagggccttg cttacagatt gcatgacacc ctcataatcc cgatgcgcgc
     1621 cgccaaccgg ttcgttgata attttgtcta caaggccgag tgaggcaagc ctttgggctg
     1681 tgatacccat gatctcggcc gcttccggcg ctttgtcggc acttttccat aatattgagg
     1741 cgcaaccttc aggggaaata accgagtagg ttgcatactg caacatctgg accacgtcgc
     1801 cgaccgccaa agccaacgca ccgcccgaac cgccctcgcc gatgatagtg caaatcaccg
     1861 gcaccctgag ttcggataaa acgtacaggt ttttcccaat agcctccgat tgcccgcgct
     1921 cttccgctcc tatgcctgga tatgcacctg gagtatcgat gaaagtgagc agcgggatcg
     1981 agaatttttc tgccaggcgc atcaggcgca atgccttgcg gtaaccttcg ggcttgggca
     2041 tgccgaaatt acgatggatt ttttccttgg tgtcgcgtcc cttctggtgc ccgatcacca
     2101 gcacgctctg gtcattgaaa cgtgccaagc caccgattat ggcgaagtca tcagcaaagc
     2161 tccggtcccc atgaagctct tcgaaatcgg tgaacaaatg ctggatatag tccagcgtat
     2221 atggcctctg tggatgccgg gccacctggg atatttgcca gggggtaagt tttgtataga
     2281 tgctttgggt gagggactgg cttttctttt gcagacgcgt gatctcggag gaaatatcaa
     2341 cagcggagtc ttcctgggca aagcgaagct cgtcaatctt tgcctcaagc tcggcgacgg
     2401 gctgctcgaa atccagaaat gttgttttca tgaagcgagg ggcaattttg aaaccgcaat
     2461 gatacaggat cgggcgaaac ccgagaaacc tccgctaagc cttcgatcga ctcttaacca
     2521 aatccgtgtg gcaagccgcc tttttttcac gcctctgaag agcatgaggc cttggttgta
     2581 tttgcgaagg ttatttgctt tgctctgtgt ttagaccggg gaatttttaa tgctcgaagg
     2641 cgcgataagt cagtcagcct ctaccatacg cccccaactg ccgataagat gggctcgctc
     2701 acgtcgttca taacctttat tcatactcga tcccacaatc tttcatatct gaaaaactcg
     2761 caatgatagc gatcgattcc gcaatgatgc gattgtgacc gaaacccttt tcacgcgagc
     2821 aaatgaggtt atggcggcag ccttctgtct gctgatcagc gcgaacgtct gtgcgcggga
     2881 tggcatcggc gagacaaacg gctcacgatc gccgttcaac ctctcggggt atgccgaagc
     2941 ctattatggt gtcgacttca acgatctcga cagcaacaga aagccgtcat tctttgtcag
     3001 ctacaccaaa aacgacaagg tttccgttaa tttgggtttt gtaaaaggat cgtacgtcac
     3061 aaacaacacc aggatcaatc tggcgttggc ggccgggagc tatgtaagag cgaattacgc
     3121 ggcagaacct gaaatactga aacatatata cgaggcaaac attggcataa aacttggggg
     3181 aagaagcaat ttgtgggtgg acttaggagt ctttccctcg cacattgggt ttgaaagcgc
     3241 gataggcaag gacaactgga cactaacgcg aggcatcggg gcggataaca cgccctactt
     3301 tgaaacgggc gccaagattg gctacacctc aaataatgaa aaatggtttt ttagcgcgtt
     3361 gatcctgaat ggctggcagc atatcaagcc tgtgccagga aattccatcc ctgccttcgg
     3421 cacacaggtt acttataaac cttctccccg gataatcctg aatagcagca cctttatagg
     3481 cagcgacaag ccggatagca acaggcagat gcgttatttt cataatttct atggcagttt
     3541 gaaattaaat gacgaacttg ccgcgatggt gggtttcgat atcggtttcg aacagaaaag
     3601 caaggaatct tcggcaatga atatgtggtt taatcccgag gccatgctga ggtacacacc
     3661 caccgcaaag atcgctctcg cgttcagagt tgaatattat gacgatcgcc atggggtaat
     3721 gatcccatcc ggcacacccg gtggttttaa aacatgggga ttctcaacca atttcgatta
     3781 caaactcaca aataacattc tttggagagt cgaagcgaga acattgcgca gcaaagacaa
     3841 ggtctttacc agggatgatg gcagcgctgc agacagcatg ctatttgtga ccacatccct
     3901 ggcggtcaat ttttgatcaa ttctccattc cgcgaatcgg gtggagcata tcaaataaaa
     3961 actctttcat aaaaaccctt ccatgccttg cttgtcttac cactttgctt ttccaggcaa
     4021 aggtacatcc ggtttttgcc tctgcccctg cgggatacct tgaatatact cggaattgcc
     4081 tgaaaaatag tataaaatac aactccttat aatcccattt tgcaggaccc aatcattgat
     4141 ttccaccgcc aacatcacca tgcaattcgg ggcaagaccc ctgttcgaaa acgtttccgt
     4201 caagttcgga gacggcaacc gttacggctt gatcggcgct aacggctcgg gtaagtcgac
     4261 gttcatgaaa attctgggcc gcgatctgga gccgacttcg ggcaatgttg ccgtcgacgc
     4321 cggcgagcgt gtcggaaagc tgcgtcagga tcagttc
//
LOCUS       DASSHJ010000052         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1409418,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000052 DASSHJ010000000
VERSION     DASSHJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409418"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="VFI43_02175"
     CDS             <1..2067
                     /locus_tag="VFI43_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7060966.1"
                     /translation="DTLDQKLWVALSCPTRGIEFDSRTLDLIDSDHDARVHANEVLGA
                     ITWAGRLLKNPDLLVSGSDYLSLAEINDGTEEGQDLTASAQYILKSLGKPEATEISIS
                     DLDDIEKFVAGLEFNGDGVIDPLQVKDAKLRATIEDIIACRGSVPNADGKAGISQEIC
                     DAFFAEVTAYVNWLDSADGDTNVHFLGEKTTEAGAAFNAVKDKVNDYFTRCRLAAYDP
                     RAAIPLSRSAEDYERIAAENLSAQNPDIADFPLATVEAGKPLPLRSEINPAWQGRIAA
                     LREQAINPILGEKENLSASEWEDLCIKFAPFEAWQEAKTTCCAERLGDSRLREIARSG
                     DKEKIDHLISQDKAVEGEVKSIQSVEKLLRFHRDLFKLVNNFVSFRSFYTGRDKAMFQ
                     LGTLYLDSRSCDLCVRVDDVAKHAEYANMSGIFLAYCECVRKDGAEKMNIAAAFTDGD
                     SDFLMVGRNGIFYDRRGRDWDATIVRILDHPISIRQAFWSPYKKLVKFISEQMEKMAA
                     ARAAAADEKLLKTAAEAAKPVAEGTPPPPTPARQAFDVGKFAGIFAAIGLALGAIGGV
                     LATIVSGILSLRIWEIPLAIIGLILLISLPSMLIAWFKLKKRNLGPILDANGWAINAR
                     ARINIPFGKSLTEMAVLPEGSRRSLVDPYADRKPIWPYYLLIAAAVIAALVTLWYFGV
                     FGNRTT"
     gene            2089..>2846
                     /locus_tag="VFI43_02180"
     CDS             2089..>2846
                     /locus_tag="VFI43_02180"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET7060967.1"
                     /translation="MDMDELNSVATAMVAKPKGLLAADESTPTITKRFGSVGVESTEE
                     TRRRYRELLFTTQGIEKYISGVILFDETLRESAHDGTPFANLLSGRGIIPGIKVDKGA
                     KPLALYPREKITEGLDGLRDRLAEYKQLGARFAKWRAVIEIDELNIPSSYAISANADA
                     LARYAALCQEAGLVPIVEPEVLMDGPHDIERCEAVTSRVLEKVFAELDAHRVVLEGIL
                     LKPNMVVAGKKCSHQPGVEEVAQATLRCLRRYVPA"
BASE COUNT          681 a          754 c          816 g          595 t
ORIGIN      
        1 gacacgctcg accaaaaact atgggtagcc ctcagttgcc ccacgcgcgg aatcgagttc
       61 gattcgcgga cgcttgatct aatcgatagc gatcacgacg cccgcgtgca cgcgaacgaa
      121 gtgctcggcg ccatcacatg ggccggccgc ttgctcaaaa atccggattt gcttgtcagt
      181 ggctcggact acctgtcact ggccgagatc aatgatggga cagaggaggg ccaggatctg
      241 actgcttccg ctcagtacat cctgaaaagc ctgggcaagc cggaagcgac tgagatctcg
      301 atctcagatc tggatgatat tgaaaaattc gtagcggggc tcgaatttaa tggtgatgga
      361 gtgattgacc ctctccaggt caaggatgcc aagctgcgcg caacaattga agatattatc
      421 gcttgtcgcg gttcagtgcc gaatgccgac gggaaagcgg gaatcagcca ggaaatctgc
      481 gatgcttttt ttgccgaggt tacagcttat gtgaattggc tggacagcgc tgatggcgac
      541 acgaacgttc attttctcgg agagaaaacg actgaagccg gggccgcctt taatgccgtc
      601 aaggataaag tcaacgacta tttcacccgt tgccgtcttg ccgcctatga ccctcgcgca
      661 gctatcccgc tgagccgctc agccgaggat tatgagcgca tcgcggcgga aaatctgtcc
      721 gcccagaacc ccgacattgc cgatttcccg ctggcgactg ttgaagccgg caaaccgctg
      781 ccgttacgct cggaaatcaa tcccgcttgg caaggccgta tcgcggcctt gcgcgagcag
      841 gctatcaacc ccattcttgg agaaaaggaa aacttgtctg cctcggagtg ggaagacctt
      901 tgcatcaaat tcgcaccctt tgaggcctgg caagaagcca agaccacttg ctgcgccgag
      961 cggttgggcg actcacgcct gcgagaaatt gcaaggagcg gggataagga gaaaatcgac
     1021 cacctgatca gccaggacaa ggcggttgag ggcgaagtca agtctatcca atcggtggaa
     1081 aaacttctgc ggttccaccg cgacctgttc aagctcgtca acaattttgt ctcgttccgc
     1141 agcttctata cggggcgcga caaggcgatg tttcagctag ggacacttta cctggatagc
     1201 cgtagttgcg atctctgcgt ccgagtggac gatgtcgcga agcacgcgga gtacgccaat
     1261 atgagcggca ttttccttgc ttattgcgaa tgtgtgcgaa aggacggcgc ggaaaaaatg
     1321 aacattgctg cggcctttac ggatggggac tcggatttcc tgatggtcgg ccgcaacggt
     1381 atcttctacg atagaagggg ccgggactgg gatgccacca ttgtacgcat cctcgatcat
     1441 cctatcagta tccggcaggc gttctggtcg ccatacaaaa agctggtcaa gttcatcagt
     1501 gaacaaatgg agaagatggc cgcagccagg gctgccgcag cggatgaaaa actcctcaaa
     1561 acagcggcag aggccgctaa acccgtagca gagggaacgc cgccgccccc gacgcctgcc
     1621 cgccaagctt tcgacgtggg taaatttgcg ggaatctttg ccgcaattgg attagccctg
     1681 ggggcgatcg ggggagttct ggcgacgatc gtcagtggaa tactgagcct gagaatatgg
     1741 gagattccat tggccatcat cgggctgata ctgctgattt cgttgccatc catgcttatc
     1801 gcatggttca agctgaaaaa acgcaatctg ggacctatac tcgatgccaa cggctgggcc
     1861 atcaacgcac gggctcgcat caatattccg tttggcaaat cccttacaga aatggcggta
     1921 ttaccggaag gctcacgccg ttcgctggtt gatccctacg ccgaccggaa acccatttgg
     1981 ccttattatt tactgatcgc tgccgccgtg atcgcagcac tagtcacgtt atggtatttt
     2041 ggagtttttg gaaaccggac aacttaaagc gtgctgcggg agtcagggat ggacatggat
     2101 gaattgaatt cagtggcgac ggcaatggtc gcaaagccca aaggtctact ggcggcagac
     2161 gaaagcaccc cgacaattac gaaacgtttc gggtcggtgg gtgtagagtc gaccgaggaa
     2221 acgcgccgga gataccgtga actgcttttc acgacacaag gcattgaaaa gtatatcagc
     2281 ggcgtgatcc tgtttgacga aaccctgcga gagagcgcgc acgatggtac acctttcgcc
     2341 aatctcctct ctggtagagg gatcataccg ggcataaagg tggacaaggg tgcgaaaccg
     2401 ctcgcgctat accccagaga aaaaattacc gaaggcctcg acggattacg cgaccggctc
     2461 gccgaataca agcaattggg agcgcgcttc gcaaaatggc gtgcagtgat agaaatcgac
     2521 gaactgaata ttccttcatc atacgcaatc agcgcgaatg ccgatgccct cgcccgctac
     2581 gcagccctct gccaggaagc gggcctcgtg cctatcgtgg aacccgaggt gcttatggac
     2641 ggcccccatg acatcgagcg gtgcgaagca gtcacttctc gcgtgcttga gaaagtgttt
     2701 gccgaactgg atgcacacag ggtcgtgctc gaggggattt tactcaagcc gaatatggtg
     2761 gtggctggaa agaaatgctc gcaccagcca ggagtggaag aagtagcgca ggctactctt
     2821 cgctgtcttc gccgctacgt gcctgc
//
LOCUS       DASSHJ010000053         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1162123,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000053 DASSHJ010000000
VERSION     DASSHJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162123"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFI43_02185"
     rRNA            complement(<1..737)
                     /locus_tag="VFI43_02185"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1374..2576)
                     /gene="tyrS"
                     /locus_tag="VFI43_02190"
     CDS             complement(1374..2576)
                     /gene="tyrS"
                     /locus_tag="VFI43_02190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7060968.1"
                     /translation="MAKPISEQLEIIKRGCGELLLEDELEQKLAANAPLRIKAGFDPT
                     APDLHLGHTVLLNKMRHFQDLGHHALFLIGDFTGMIGDPSGKNATRPPLTREQVVENA
                     KSYQNQVFRILNPEQTEVVFNSAWMDRLGAADVIRLAATHTVARMLERDDFGKRYRSN
                     KPIAIHEFLYPLIQGYDSVALKADVELGGTDQKFNLLMGRELQKHFGQPPQCILTMPL
                     LEGLDGVNKMSKSAGNYIGITESSTDIFGKLMSISDELMWRYIELLSFEPLSVIRQWQ
                     SEVEQGRNPRDIKVLFAQEIVARFHGKQKAERVLADFEARFKRGATPDDIVERLLHAD
                     KEQLPIPQLLKLAGLTASTTEALRMIEQGGVKINGERVSDKTLSVGRGQTAVLQVGKR
                     KFARVTVK"
     gene            complement(2593..3207)
                     /locus_tag="VFI43_02195"
     CDS             complement(2593..3207)
                     /locus_tag="VFI43_02195"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7060969.1"
                     /translation="MSNQTGDAKHTLPPLPYPENALEPVISANTLSFHYGKHHKAYVD
                     NLNKLIVGTEFADKSLEEIITETAGKADKAGIFNNAAQVWNHTFYWNSLSPNGGGEPP
                     SSLKQKIEASFGGLDAFKKEFANAAVTQFGSGWAWLVKDGDGLKIIKTSNADMPLTKG
                     LKPLLTIDVWEHAYYLDYQNRRPDYANALIDKLINWGFAEDNDV"
     gene            3573..>4615
                     /locus_tag="VFI43_02200"
     CDS             3573..>4615
                     /locus_tag="VFI43_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET7060970.1"
                     /translation="MNEPLITCPRCKAEIPLTESLAAPLITATRQQYEQQLLQKDGEI
                     AKRERGIREREKEVAEAKRTLDDQVASQVAKQLNSERSRLIAEETRKAKLAAAAELDS
                     RTREIAELHEVLKGRDEKLAAAQKAQAELVKKQRELDDARRELELTIEQRVQHGLTAA
                     RMLAKQEAEESLRLKVMEKDQTIAAMQQKIEDLKRRAEQGSQQLQGEVQELMLESLLR
                     AKFPFDAIEPVPKGEFGGDVLHRVIATTGQTGGTILWETKRTRNWSDLWLAKLREDQR
                     AAKAEIAVIVTQTLPKGIETFELIDGIWVTHPRAALPVALVLRHSLLELALARQSSEG
                     LQTKTELVYQYLTG"
BASE COUNT         1073 a         1292 c         1167 g         1083 t
ORIGIN      
        1 cccagggggc tgccttcgcc atcggtgttc ctccacatct ctacgcattt cactgctaca
       61 cgtggaattc cacccccctc tgccacactc tagccttgta gtttcaaacg cagttcccag
      121 gttaagcccg gggatttcac atctgactta caaaaccgcc tgcgcacgct ttacgcccag
      181 taattccgat taacgcttgc accctacgta ttaccgcggc tgctggcacg tagttagccg
      241 gtgcttcttc ttccggtacc gtcagtgatc acaggtatta gccgtgaccg tttcgttccg
      301 gctgaaagag ctttacaacc cgaaggcctt cttcactcac gcggcatggc tggatcaggg
      361 tttcccccat tgtccaaaat tccccactgc tgcctcccgt aggagtctgg gccgtgtctc
      421 agtcccagtg tggctggtcg tcctctcaga ccagctactg atcgtcgcct tggtaagcct
      481 ttacctcacc aactagctaa tcagacatcg gccgctcaaa gagcgcaagg tcttgcgatc
      541 ccctgctttt ctccgtagag attatgcggt attagcacac ctttcggtgc gttatccccc
      601 actcgaagac acgttccgat gcattactca cccgttcgcc actcgccacc agggttgccc
      661 ccgtgctgcc gttcgacttg catgtgtaaa gcatgccgcc agcgttcaat ctgagccagg
      721 atcaaactct tcagttcaat actggtaaaa ctctcgcttg acgaatttcc cgatcccggc
      781 ttcctaagaa acaaggttgc cagaatcgta ttacatcgtg cgagcacttc aactttaaag
      841 acctgactat gtccccagag gggatagcca gttgtcccgg tcaagtgccc acacctatcg
      901 gctgtaaatt tttaaagagc agtgctggcc ttaaacaaac ctatcaaggc aagcaacgaa
      961 aggcggcatt atacagatac caggaacgcg gtcaagagtt tttatttctt tcttcgtcag
     1021 gtaaccgcac cccgtcgaat ttttcctgct tgctgctttt tacttatcct gctgtttcac
     1081 gccttcgctg tcaggagcca ttcgaccacg ggccacctaa acaagttcca cggtttcagg
     1141 ttttatctca acctggccga ggcatatgaa gctcggttgc tttctccagc aagcctgaga
     1201 gtatcacccc gattgccaaa cagcaagtct attacattaa tatagacagt acactcgtac
     1261 gttggttcac gcctccttgc gcttcctcgc aacgtcctct ccctgatagg atgaaggaag
     1321 aaggaggagg gaaaagcggc cgggcaagca gaggcggcgg taacagcacg tatttatttt
     1381 accgttaccc tggcgaattt ccgcttgcct acttgcaaca ctgcggtttg accacggcct
     1441 actgaaaggg ttttgtcgct cactctttcg ccgtttatct ttaccccgcc ttgctcgatc
     1501 atccgcaggg cttcggtcgt actcgcagtc aaccccgcca gcttgagcaa ttggggaatg
     1561 ggcaattgct ctttgtcagc gtgcagcagt ctttccacaa tatcgtcagg agtcgctcca
     1621 cgcttgaacc gcgcctcgaa atcggctagc acacgttcgg ccttctgttt gccgtgaaaa
     1681 cgtgcaacaa tttcttgcgc gaacaggacc ttgatgtctc gggggttgcg accctgctcg
     1741 acctcgcttt gccactgccg gatgacgctc aagggttcga acgacagcaa ctcgatatat
     1801 cgccacatca actcatcgga aatggacatt aacttgccaa aaatatcggt cgagctttcg
     1861 gtaatgccaa tatagttgcc tgctgatttc gacatcttgt tcacgccatc cagtccttcg
     1921 agcaacggca tggtgaggat gcattgaggc ggctgcccaa aatgcttctg cagctcacgg
     1981 cccatcagca aattgaactt ctggtcagtt ccgccgagct ccacatctgc cttcagagcg
     2041 accgaatcgt agccctggat gagcggatat aaaaattcgt gaatcgcgat aggcttgttg
     2101 cttcgatagc gcttgccaaa atcatcgcgc tccagcattc gggcaacggt atgcgttgcg
     2161 gccagtctta tcacgtccgc ggctcccaat cgatccatcc aggctgaatt gaatacaact
     2221 tcggtctgtt cggggttcag tatccgaaaa acctggttct ggtaggactt tgcattctcg
     2281 acgacctgtt cgcgcgtgag aggaggacgt gtggcgtttt tgccgctggg atcaccgatc
     2341 atccccgtaa aatcaccgat cagaaacagg gcatggtggc ccagatcctg aaaatggcgc
     2401 attttattga gtaaaacagt atggcccagg tgcaggtccg gcgccgtagg atcaaacccg
     2461 gccttgatcc ggaggggagc gtttgcagcg agcttctgct ccaactcgtc ttccagcaga
     2521 agctcgccac agccgcgctt gataatctcc agttgttccg aaatcggttt cgccacaatc
     2581 gatcaggatg ctttatacat cgttatcctc agcaaaaccc cagttgatca gcttgtcgat
     2641 caaggcgttc gcataatcgg gacgccggtt ctgataatcg aggtaatacg catgctccca
     2701 cacgtcgatt gtcaggagag gcttcagccc cttggtcaag ggcatgtctg cattgctcgt
     2761 cttgattatt ttcagaccgt ctccatcctt gactagccat gcccaaccgc ttccgaattg
     2821 agtgaccgct gcattcgcaa actctttttt aaaggcatcc aggccgccaa acgacgcctc
     2881 gattttctgc ttcagggaag aaggaggttc tccgcctcca ttgggcgaca ggctgttcca
     2941 atagaacgta tggttccata cttgtgccgc attattgaag atgcccgctt tgtccgcctt
     3001 gccagcggtt tcagtgatga tctcctccag cgacttgtcg gcgaactcgg tgcccactat
     3061 aagtttattg agattgtcga cgtatgcctt gtgatgcttg ccataatgga aacttagcgt
     3121 atttgccgag atcaccggtt caagagcgtt ttcaggatag ggcaacggtg gcagcgtatg
     3181 tttcgcatct ccagtctgat tactcatctt cttctccttt gaagtgacat aaaatcggag
     3241 cccccttatt ctcgatccag agagaacctg ggaaccgaac gcgtgagata caatgctgtc
     3301 tctgcgaaag gcccctctcc gttcttgtag taccagtcca aatgatagac gctaaagagg
     3361 ggtgtgagtc aggtttcgtg caaacacaag cgggaaggac tgaggcagcg cgcttaggtg
     3421 tacatgattc gcgagtgtct gttttgtggg acaagtatat cggaaaaagg tctccctcac
     3481 acatgccggc caatcctgac ctggttgccc gcaagtactc ggcaccgcaa ctgaaagggc
     3541 gatcgtccgg caccaatttc cagggaaata tcatgaatga acctttgatc acctgcccca
     3601 ggtgcaaggc ggaaattccg cttaccgagt cgcttgccgc accacttatc actgcgactc
     3661 gccagcaata cgagcaacag cttctgcaaa aggatgggga aatcgcgaag cgggaacgcg
     3721 gtattcgcga gcgtgaaaag gaggtggcgg aagcaaagcg cacgcttgat gatcaagtag
     3781 ccagccaggt cgccaagcag ttgaattctg aacgttcacg cttgattgcg gaggaaacgc
     3841 gaaaggcaaa acttgctgcg gctgccgaac ttgacagcag aacgcgagaa atcgcggagc
     3901 ttcacgaggt gctcaagggt cgcgacgaaa aactggccgc cgctcaaaaa gcccaggcgg
     3961 aactcgtgaa aaagcagcgg gaactcgatg atgcgagacg ggaactcgaa cttaccattg
     4021 agcagcgggt ccagcatggg ctaaccgcgg cgcgcatgct tgcaaagcag gaagcggaag
     4081 agagcctcag gctaaaagtg atggaaaaag accagaccat agcggccatg cagcaaaaaa
     4141 tcgaggatct gaagagaaga gcggagcagg gctcacagca attacagggg gaagtccagg
     4201 aactcatgct cgagagcctt ttgcgcgcga agtttccttt cgatgcaatc gagccggttc
     4261 ccaagggaga gttcggggga gatgttcttc accgtgttat agccacaacc ggacaaaccg
     4321 gaggcacaat tctttgggag accaaacgga cgcgaaactg gagcgatctc tggctggcca
     4381 aattacgcga ggaccagcgc gccgccaaag cggagatcgc ggtaatcgtg acccagactc
     4441 tgccaaaagg catcgaaacg tttgaattga tcgatggcat ctgggttacg caccctcgcg
     4501 ccgcactgcc cgtagcgctt gtcttgcgcc attcattgct ggagcttgcg ctcgcgcggc
     4561 agtcttccga aggcttgcaa accaagaccg aactggttta ccagtacctc acggg
//
LOCUS       DASSHJ010000054        22813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1383518,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000054 DASSHJ010000000
VERSION     DASSHJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 22813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22813
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383518"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..2221
                     /gene="htpG"
                     /locus_tag="VFI43_02205"
     CDS             293..2221
                     /gene="htpG"
                     /locus_tag="VFI43_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET7060971.1"
                     /translation="MEATVTKEHLNFQTEVKQLLKLMIHSLYSNKEIFLRELISNASD
                     AADKLRFEALTDGALYESDSDLKIRVSYDPKERTITVTDNGIGMSRQEVIDHIGTIAK
                     SGTREFFSSLTGDQARDAHLIGQFGVGFYSAFIVADQVTLITRRAGLTPEHGVRWESG
                     GEGEYTLETVEKADRGTQVILHLREGEDDLLNGWRLRSIIRKYSDHITLPIVMKKEQW
                     SQEKNENTITDEDETVNQASALWARPKSEISREQYEEFYKHVAHDFEPPLAYVHAKVE
                     GKQEYTLLLYIPSRAPFDLYDREQRHGIKLYVRRVFIMDDAKQLLPSYLRFVRGVVDS
                     NDLPLNVSREILQQSKDTETIRAGAIKKVLGLLEDLAQSEQEEEKSKYKTFWKEFGQV
                     IKEGVAEDYANRERIAKLLRFTSTHADSDEQTVSLADYISRMKEGQEKIYFVTADTLK
                     AARSSPHLEIFRKKGIEVLLLHDRVDEWLVAHLTEFEGKPLQSVAKGSLDLGKLEDEA
                     EKKEQDKEAGEYKELTEKIKGVLGDQVKDVRVTLRLTESPACLVADSQDMGGNLERLL
                     KSSGQKVDHAKPILEINPHHPMIQSLKYEQKHFDDWSHILFDQALLAEGGQLDDPASF
                     VKRINELLFTKPSVIITP"
     gene            complement(2248..3168)
                     /gene="nhaR"
                     /locus_tag="VFI43_02210"
     CDS             complement(2248..3168)
                     /gene="nhaR"
                     /locus_tag="VFI43_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HET7060972.1"
                     /translation="MINYKQLHYFWTVAKAGSIVRASERLHLTPQTLSGQIGLLEEAL
                     GVPLFSRVGRRLELTETGRLALSYADEIFQIGNELEMALRSRPESPAIQFRVGVDDAV
                     PKSIAYKLLTPAMSLDESVRIVCREDKLVRLTVELAIQRLDMVLADRPMPPGSNVKGY
                     SHKLGECGLTFFADPKLASRFKGKWPNVLNGAPLLIPGKEAAVRAPLMRWLDMRQLKP
                     RIVGEFDDSALMQAFGQAGNGIFIAPSVITNEVQREHGVAVVGQTDEVAEQFYAISLE
                     RRLTHPAVVAINDAAQKKLFGNPLTPPSPG"
     gene            3707..4993
                     /locus_tag="VFI43_02215"
     CDS             3707..4993
                     /locus_tag="VFI43_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7060973.1"
                     /translation="MAPGGPVFAPTGPGVDRAKGEAREAAKASGLAGAGPVLSIDTVQ
                     RAGLEANGLVRAARSQIDMAEAGVVGATAYPNPQLTFMGGPQHARIPSALPANIHRQF
                     TVAQTIENPFLRSARIGSAEAAVDSSRANLDQVRADLAAQLRVRAYELLLRQEIARME
                     ASIYDIMQDVRRRIAIGVSVGETARFELTRAEAEVMNAASRKEAAELNSERARIGLMQ
                     LTAGALPSNFQIKGSLYDPVTVPALEELRQEVPAVNPEVLRLEAEQNRARLRIDQERA
                     SVFPSVQILLSNFQEAQYTSNLAGMHVTIPLFYRRRGEIDTAVADSARVKDTLDYRRY
                     EISQLLESAWQALQIARRRVEMFEGGIVKEAETAFRIAQSAYVLGERGLIEVLDTQRV
                     LRGILAESLQARFDLHTAAAEIDRLRAHYPKEQITE"
     gene            4990..6156
                     /locus_tag="VFI43_02220"
     CDS             4990..6156
                     /locus_tag="VFI43_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7060974.1"
                     /translation="MKAGLIFFISIVASLLMSGCDNGEPPASPEKTVKQETETARDVN
                     SITIRPELVPRIKIGHPTMVDLADKLQVPSRVEVDEERLVRVGSYVTGRIIDLYVTLG
                     ESVNAGEALARITSPELTQAQLAYLRASSRTLLAQKGAERAHRLLEADVIPVAEVERR
                     ESELEVARAELEAAKDQLRLLGVDSSVLKELARHARILPSVTINAPRNGIIIGRNVIV
                     GQVVQPADQLFQIADLSSVWVVGDVPEQIARNVRVGQHVEIHVPALGDRGFDGLIIFV
                     ADTVDPATRTVMVRTMVENPQRRLKPDMLANMHITDTLHKSLVVPEQAVVREGNRDYV
                     FLSKGNNRFLRVPVELGPEVADMRPVINGVTVDQSIVVEGAFHLDNERKLAELE"
     gene            6160..9378
                     /locus_tag="VFI43_02225"
     CDS             6160..9378
                     /locus_tag="VFI43_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET7060975.1"
                     /translation="MLASIVRTMLKQRLLVVVVALGLCAAGVFAAKNLTVDAFPDVTN
                     IQVQVATVAVGRSPEEIERLITVPVEIAMTGLPGLVEMRSLNKSGLSLITLVFTDQTD
                     VFFARQLVMERLIEVSPRLIPGITPVLGPVSTGLGEVYQYTIEHPDDGKRPLSVEELT
                     ERRTIQDWVVRPMLRSIRGVAEVNSIGGHVKEYQVFVDPNKLRHHDLTLAAVDRAIAS
                     NNANASGNILPLHDEQYLIRGVGLIRTLDDIRNIVLKEVEGVPIFVRDVAEVKFGGEV
                     RVGASIKEGYTEAVTGIVMMLRGGNAKQIVEQVKEKVAEINERKLLPHGLQIVPFYDR
                     TDLVDAALGTVYKTLMEALVLVVAVLFVFLGNLRTSLVVCSTLVITPLVTFMVMNHYG
                     MPANLMSLGGLTIALGMMVDPTVVVVENIFQQLGQARRTGESKFDIIVRATAEVGTPV
                     IFGVIVIILVFLPLMTLQGMEGKMFAPLAITIAISLTVALIVSVLLSPVLSAYLLKGG
                     SEKDTKIVAVLKSVYLRVLNRALGSQKATIVIALVLLSLAFVVYPLLGKSFIPVMREG
                     SITPVIIRVPSISLEESIELEAEAMRLIAAIPGVKTVVSKLGRGESPADPASPNESDP
                     IAALDLEGSGRTQQEIEEDIRKALTVLPGVNIVLSQPIEQRVDEMVTGVRSQVAVKIF
                     GDDLEELRKLSEQVARIIRSTRGARDIRIERLSGQQELTIDIDRRAIARHGLNVADVN
                     ELISTAIGGKAVTQVFEGERRFTLLLRFPERFRHDIEAIRDLLLRPPGNGGTNGLLAR
                     GGQLVPLSAVADIRVVDGPAVVSREFAKRRVVVGANVHERDLGGFVAELQERTAKEVI
                     LPPGYYFAWGGQFENMERAMATLSVIVPVTLAVIFFLLFMLFNSIKLAVLIFLALPFA
                     SVGGVVGLYLTGEYLSVPASVGFIAVWGTSILNGVVLIEFIRTLRFQGLSVPEAVRMG
                     CEQRFRPVLMTAATTVLGLAPFLIATGLGSEVQKPLAIVVISGMITATMMTMVLMPLL
                     YRWFDDQPKLEEEAIDDDTSPRLSLLGRIRSRFRKNSPAVGT"
     gene            9448..10335
                     /gene="argB"
                     /locus_tag="VFI43_02230"
     CDS             9448..10335
                     /gene="argB"
                     /locus_tag="VFI43_02230"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET7060976.1"
                     /translation="MPLSPSTAKDKARILAEALPYIRRFHGKTIVIKYGGNAMVEENL
                     KQGFARDVVLLKLVGMNPVIVHGGGPQIDHVLKRLGKHGEFIQGMRVTDAETMNVVEM
                     VLGGVVNKEIVNLINQQGGRAIGLTGKDGNFICAKKMRVRNKEKAGEWLDIGQVGEIE
                     SICPTLIELLEANDFIPVIAPIGVGRNGESYNINADLVAGELAKVLCAEKLILLTNTP
                     GVLDNSGNLLTGLTAQKVDELIADGTISGGMIPKISSALDAVKQGVKSCHIVDGRVED
                     GLLLEVLTDEGVGTLIKAN"
     gene            10407..11027
                     /gene="slmA"
                     /locus_tag="VFI43_02235"
     CDS             10407..11027
                     /gene="slmA"
                     /locus_tag="VFI43_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HET7060977.1"
                     /translation="MPGRSGDRKDQILQVLAEMLESPRPEKITTAALAAKLNVSEAAL
                     YRHFASKAQMFEGLIEFIEKTLFSLINKLTAEEKSGLSGPGQVEGILSLLLGFARKNP
                     GMTRVLIGDALVNENDRLQARVNQLHDRLETTLKQALRFQTNDKENPVSIDVSAQANM
                     LMCYVVGRWHQFAKSGFKRDPMEYWEAQRKEVLLGLAIKVPAHNRG"
     gene            complement(11024..11779)
                     /locus_tag="VFI43_02240"
     CDS             complement(11024..11779)
                     /locus_tag="VFI43_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HET7060978.1"
                     /translation="MNDQQLLRYSRHILLPQIGITGQESLIRSHVLIIGAGGLGSPVA
                     MYLAASGVGKLTICDSDAVDLTNLQRQIVHSTDLIGAPKASSAQKTLARINPEVKVVA
                     LDERIDENRLQELALHANVVVDASDNFSTRYAINRACTILKKPLVSGAVVRFDGQVSV
                     FDLRYPNSPCYHCLFPENGDDQDMRCAVMGVFAPLVGIVGCMQAAETLKILLEIGKTL
                     NGRLLMLEGLTMEWRSTKLNKDPSCEVCRQSGR"
     gene            complement(12201..13637)
                     /locus_tag="VFI43_02245"
     CDS             complement(12201..13637)
                     /locus_tag="VFI43_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7060979.1"
                     /translation="MGNKMRQFGLVIFGAIAGVMLSLNFSAVANKDTQEVLHPLPVEE
                     LRAFTEVFGRVKSDYVEPVEDKKLITEAINGMLTGLDPHSAYLDSDAFKELQIGTQGE
                     FGGLGIEVSMEDGFVKVISPIEDTPAFRAGIKPGDLIVKLDDTAVKGLSLTDAIKRMR
                     GKPNTPITLTIVRKGESKPMAFPLVRAVIKIQSVKSKMIEPGYGYIRITQFQEQTGEN
                     LTKAIDKLFKESPVPMKGLVLDLRNDPGGLLNGAVAVSAAFLPANSLVVYTDGRTEDA
                     KMKLKASPEFYLRDTKNDYLKRLPPGIKTVPMVTLVNGGSASASEIVAGALQDHKRSI
                     VMGTQTFGKGSVQTILPLGNNTAIKLTTARYYTPNGQSIQARGITPDIVDESAKDETE
                     RLREADLDRHLSNGKDDEIKQSLEAIKNAPAPRPVIKTPKSEVDEEKNKKREPPAEFG
                     SSGDLLLAQAMNYFKENAAKKNAAKVAN"
     gene            complement(13664..14929)
                     /locus_tag="VFI43_02250"
     CDS             complement(13664..14929)
                     /locus_tag="VFI43_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7060980.1"
                     /translation="MALLVMMLPVPHLLHAAAKGGKEEPRKVDKEDLKKVDKEDLKQL
                     RSRIEALQKELADTEESKSEVADALRDSDRAISATNRQLNKLAQEQDKAVEKLGRLRA
                     QSEQLARDIEIQQSQLEKLLNHRYIAGGGQKEYLALLLNHQEPNQIARNLHYYSYISR
                     ARSETIDALRDNLEQLDSLALESQEKSAEIAKIKVKKADYKKQLEREKTDQASLLTRI
                     SLQADQQRREISRLKQDEERLTRLVEKLAKMLARKNRAARSAPAVRPSAPSSSTAPLT
                     NHSVPDGSTDGNPFAALKGRLSLPVRGELANRFGSPRHEGGLTWKGLFIRSPTGADVK
                     AIAAGRVVFADWLRGFGNLLIVDHGANYMSLYGNNEAIHKQVGDAVAGGDTIATVGDS
                     GGVSKGAGLYFELRHQGKPFDPLNWVRIK"
     gene            complement(15000..15755)
                     /gene="gpmA"
                     /locus_tag="VFI43_02255"
     CDS             complement(15000..15755)
                     /gene="gpmA"
                     /locus_tag="VFI43_02255"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET7060981.1"
                     /translation="MKKLVLLRHGESTWNKENRFTGWTDVDLTRTGLEEARNAGILLR
                     GGGFRFDVAYTSVLKRAIRTLWIVLDEMDQMWIPVNLSWRLNERHYGALQGLNKLETA
                     LKYGEEQVLIWRRSYSIRPPALTCDDDRYPGFDPRYHDLDPSEIPLTECLEDTVARFL
                     PYWNETIAPQVRAGQRVLISAHGNSLRALVKYLDNLSAEEVLELNIPTGVPLVYELDD
                     DLKPIRSYYLGDQTQVEQAMRAIANQGKIAPHL"
     gene            16090..16497
                     /locus_tag="VFI43_02260"
     CDS             16090..16497
                     /locus_tag="VFI43_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7060982.1"
                     /translation="MSFVQNNIALIVAAAASGILLLWQIFRKSRNEIGTMVAVQLINY
                     KEALVLDVRERSEFESGHIPNAKHIPADKLEERLQELEKFKNKPIVIIHRAGVSTTGK
                     AGSILRNNGFAHVHDLGGGIDAWKQANLPIVKK"
     gene            16508..16765
                     /gene="grxC"
                     /locus_tag="VFI43_02265"
     CDS             16508..16765
                     /gene="grxC"
                     /locus_tag="VFI43_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HET7060983.1"
                     /translation="MAKVIMYSTAFCPYCVMAERMLRARGVTEIEKIRVDLEPARRSE
                     MIEKTGRRTVPQIFIGNTHVGGYDDLAKLDRSDGLMKLLAV"
     gene            17044..17553
                     /gene="secB"
                     /locus_tag="VFI43_02270"
     CDS             17044..17553
                     /gene="secB"
                     /locus_tag="VFI43_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET7060984.1"
                     /translation="MESNTNSQPGGEQPQPLFSIEKIYVKDLSLEIPNAPRIFLERET
                     PEVNIQLHTKAERIDEGMFESVLTITVTAKIKDKTMFLVEVQQAGVFQIRNVPEADME
                     PVLGIACPNVIFPYLRETVSDIVTRAGFHAVVLNPVNFEALYYQGKQQGEKSLSTEAP
                     DGDSQKVTH"
     gene            17560..18057
                     /locus_tag="VFI43_02275"
     CDS             17560..18057
                     /locus_tag="VFI43_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7060985.1"
                     /translation="MKYAGAVLAFLGDRTFRRAVPAMCGFLLAFQMAGPAFAALEFLS
                     IKEPAVIMYDAPSLKADKLYVASKHLPVEAVVKVEGWVKVRDSSGSLAWVEEKQLSDA
                     RYVIVTAPSAEIHQTANSNSPLVFRALQNVVLELLEPAASGWAKVEHPDGQTGYVRTT
                     QVWGT"
     gene            18054..19055
                     /locus_tag="VFI43_02280"
     CDS             18054..19055
                     /locus_tag="VFI43_02280"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7060986.1"
                     /translation="MRIAVLGAGAWGTALAISLSSNNSSSHEVMLWTRDPEHLAELES
                     DHINRRYFPAFPLPDCLGLTSVLGAAIEFAELALIAVPTYGLRETLRGIAAHGKDLPL
                     IWGCKGFESLSAKLPHQVVQEEYGNAAPYGVLSGPSFAQEVAQGLPTALTLASHDEQF
                     SRVMAAELRTPRLRVYSCKDIVGVETGGAIKNVISIAAGISDGMGFGYNARAALITRG
                     LAEIARLGLKLGGRMETFMGLTGAGDLILTCTGDLSRNRRVGLMLAAGRCLPDILHDL
                     GHTAEGVHTAAEVGRLSEILKVEMPITKAVCSILYEGTPAGLAVEALLNREPRNEIY"
     gene            complement(19518..19982)
                     /gene="trmL"
                     /locus_tag="VFI43_02285"
     CDS             complement(19518..19982)
                     /gene="trmL"
                     /locus_tag="VFI43_02285"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HET7060987.1"
                     /translation="MLDIVLYQPEIPPNTGNIIRLCANTGTRLHLVKPLGFALEDKQL
                     LRAGLDYHEFASIMVHESWEACAAHMQGRRIFPVSTKGMQRYDRAAYSNGDVFLFGPE
                     TRGLPMWLLESLPSQYRIRVPMVAGSRSLNLSNTVAVVIYEAWRQLGFQDGI"
     gene            complement(20149..20889)
                     /locus_tag="VFI43_02290"
     CDS             complement(20149..20889)
                     /locus_tag="VFI43_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7060988.1"
                     /translation="MNNRLSFVQALLGSDCLLCGAPAGPAFIPGLDPGADSGSADFCL
                     PCRKTLPYLSSSHCSVCAVPVTGASTCGACLAKPPAFDRTVAAFEYAFPVDALLQSLK
                     YRSNLAMARVLAGFLSARITSTVNPAELPDYIIPMPLHAKRLRERGFNQAVEIARYVS
                     KSTHIPLFARACQRTRDTPSQTELPWKEREKNIRGAFTCEADFSGKRVVILDDVMTTG
                     ASVNELAKLVSNRGAMRVSVWVVARTLP"
     gene            21019..22047
                     /gene="bioB"
                     /locus_tag="VFI43_02295"
     CDS             21019..22047
                     /gene="bioB"
                     /locus_tag="VFI43_02295"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET7060989.1"
                     /translation="MSQFHFDVTDNRETCGNGTSDSSATGPGASAGSLRWSIPAIESL
                     LDSPFNDLIYRAQVVHRQHHDANAVQLSTLISVKTGGCPEDCGYCPQAARYHTNVENQ
                     DMLRLDEVVASASAAKAQGATRFCMGAAWRGPKQRDIEKMTEMIGAVKALGLETCVTL
                     GMLKPGQAEQLREAGLDYYNHNLDTSPEFYGEIITTRTYEDRLDTLGKVRSAGINICC
                     GGIVGMGESRRARAGLIAQLANLDPYPESVPINHLVQVEGTPLHGTETLDPLEFVRTI
                     AAARITMPKAMVRLSAGRREMSDAVQALCFLAGANSIFYGDKLLTTGNPEAERDKALF
                     GKLGLSSI"
     gene            22122..>22813
                     /locus_tag="VFI43_02300"
     CDS             22122..>22813
                     /locus_tag="VFI43_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7060990.1"
                     /translation="MSADLIGGLAEQLRSREKKNLFRERVALEGPQDIRVSIDGREHL
                     AFCSNDYLGLANHPELIVAACEGARRYGVGAGASHLISGHSLAHDVLEDALARFIGFP
                     RALLFSTGYMANAGVVAALMGRTGAIFGDKLNHASLNDAVLVSKARFSRYRHGDLAGL
                     KHQMAASNARSKLVLTDAVFSMDGDLAPVARLLELCEKYDAWLMLDDAHGFGVLGEKG
                     KGILGHVDVQSPR"
BASE COUNT         5149 a         6012 c         6509 g         5143 t
ORIGIN      
        1 agtgccaatt cccgcgcgtt aggaacctct gcccagagtt cgatggaaaa cccggggggg
       61 agcttgatac ggtcgagcgg caacgggcgt gactgggcat gagtgccggg cgcgaaggag
      121 aatagtgata tagcgataaa atgagtgatg agctttatca tggagtatta gtgttgaacg
      181 gcttttcgtc gggcgaatga atgaacggga ctggcgaaaa tgcgttgaaa tcgccgcttg
      241 aaaatctcga ttccgccctc attattttag cctgattctt tcaggaggaa acatggaagc
      301 aacagtgacc aaagaacatt tgaatttcca gacggaagtc aagcaattgc tcaagctgat
      361 gatccactcc ttgtacagca acaaggaaat cttcttgcgc gagctcattt ccaacgcgtc
      421 ggatgctgcc gacaagctcc gcttcgaggc gttgaccgac ggcgctctgt acgaatccga
      481 ttcggacctc aagatccgtg tcagctacga tccgaaggag cgcaccatca cggtcaccga
      541 taatggcatt ggcatgtccc ggcaggaagt catcgatcat atcgggacga tcgcgaagtc
      601 tggaacgcgg gaattcttca gttcgttgac gggcgatcag gccagggacg ctcacttgat
      661 cggtcagttc ggcgtaggct tctattcagc ctttatcgtg gctgaccagg tcaccctcat
      721 tacccgccgc gctgggctga cgcccgaaca tggcgtccgc tgggaatcgg ggggagaagg
      781 cgagtatacg cttgagaccg tcgagaaagc ggaccgcggc acccaggtta ttttgcattt
      841 gcgggaaggc gaggatgact tgctcaacgg ctggcgtctg cgttcgatca ttcgtaaata
      901 ttcggatcat atcacccttc ccatcgtgat gaaaaaagag cagtggtctc aggaaaagaa
      961 tgagaacacg atcaccgatg aggacgaaac cgtcaaccag gcgagtgcgc tctgggcccg
     1021 ccccaagagt gagatctcgc gtgagcagta tgaggaattc tacaagcatg tagcccacga
     1081 tttcgagcct ccgctggcct acgtgcatgc caaggtcgag ggcaagcagg aatatacgct
     1141 gctgctttac atcccgtccc gtgcaccctt tgatttgtac gatcgcgagc agcgccacgg
     1201 catcaagctg tacgtccgcc gggttttcat catggacgac gcgaagcagt tgctgccaag
     1261 ctatctgcgt ttcgtgcgtg gcgtggtcga ttcgaacgat cttccgctca atgtttctcg
     1321 cgagatactg cagcagtcga aggacactga aacgattcgg gcaggggcaa taaagaaagt
     1381 gcttgggctc ctggaggatc tcgctcaaag cgagcaggaa gaggaaaaat caaagtacaa
     1441 gacgttctgg aaggagttcg gccaagtcat caaggagggt gtagcggaag attacgccaa
     1501 tcgcgaacgc attgccaaac tgctgcgctt tacgtccacc cacgcggatt cggacgagca
     1561 aacggtatct ctggccgact acatcagccg gatgaaagaa gggcaggaaa aaatatactt
     1621 cgtcactgcg gacaccctga aagcagccag gagtagccct cacttggaaa tcttccgtaa
     1681 aaagggcatc gaggtattat tactgcacga tcgcgtcgac gaatggctgg ttgcgcatct
     1741 gacggagttt gaaggcaaac ccctgcagtc cgtcgcaaaa ggcagtcttg atctcggcaa
     1801 gctggaggac gaggcggaaa agaaggagca ggacaaggaa gcgggggaat acaaggagct
     1861 tacggaaaaa ataaagggcg tgctcggcga tcaggtgaag gacgttcggg tgacattgcg
     1921 actgacggaa tctcccgcgt gcctggtggc agacagccaa gacatgggcg gaaacctgga
     1981 acggttgctc aaatcgtcag ggcagaaggt ggaccacgcc aagccgattc ttgaaatcaa
     2041 cccgcatcat cccatgatac aaagcctcaa gtacgagcag aagcacttcg acgactggag
     2101 ccatatcctt tttgatcagg ctttgcttgc ggagggcggg cagctcgatg atccggccag
     2161 tttcgtcaag cggatcaatg aactcctgtt taccaagccg tcagtcatca ttacgccttg
     2221 accggccggg ctgtttttga atatccttca gccaggactt ggtggagtta acgggttgcc
     2281 gaacagtttc ttctgtgccg cgtcgttaat cgcgacgact gccgggtggg ttaatcgccg
     2341 ctccagagag atggcataga actgttcggc aacttcatcc gtctgtccga cgactgcgac
     2401 gccatgctca cgctgtacct cgttggttat aacggacggg gcgatgaaaa ttccattacc
     2461 ggcctgcccg aaagcctgca tgagcgcgct atcgtcaaac tctccgacga tgcgcggctt
     2521 tagctggcgc atgtccagcc aacgcatgag tggcgcgcgg accgcggctt cctttcctgg
     2581 gatcagcaaa ggcgccccgt tgagaacatt cggccatttc cccttgaacc tggaagccag
     2641 ctttggatcg gcaaaaaatg tgagtccgca ctccccaagt ttgtggctat agcctttcac
     2701 attagagcct ggaggcattg gccgatccgc aagcaccatg tccaggcgct ggattgcgag
     2761 ctcgaccgta aggcgcacca gcttgtcttc gcgacagacg atgcgtacgg actcatcaag
     2821 gctcattgca ggggttaaca atttgtaggc gatcgatttt ggaacggcgt catcgacgcc
     2881 gacacgaaat tgaatggctg ggctctccgg tcggcttcga agggccattt ccagctcgtt
     2941 gccgatctgg aagatttcgt ctgcataaga aagcgcaagc cgccccgtct cggtcaattc
     3001 aagccgtctg ccaacacggg aaaacaacgg gacgccaagc gcctcttcca gcaagccaat
     3061 ctggccgctg agggtttgcg gcgtaaggtg aaggcgttca ctagcgcgaa caatgctgcc
     3121 tgccttggcc accgtccaga aatagtgcaa ttgcttgtag ttgatcatgc ggcgagcgag
     3181 gttacgtgaa acggagccgg atgcgatagt tcgtaaaaac cgatcatatc atcagaagta
     3241 tacgactttt cttgatgcaa attctcattt aaactgccat tatgaatttt ttaaacccgg
     3301 attttttaac cccggaagtt ctcaaaccca aagtccttaa tccgggaatc tccgacccgg
     3361 aaatccttga ctcttgtttc caacccgttt tccggtaaga ccgtggcgaa ccgggatcag
     3421 gctcttttgg aaaattttta aaaggcagtc gatgcgagca ccggtagtca gttgcatttt
     3481 gctgagcttg attttcagtt tccgatttac gtctgcttcg gctcaacagc cctcaacgat
     3541 accttttatt accgctcccg agccatcttc tccggcgccc ttgtactctc cagagccatc
     3601 tcttggtccg cgcacgggga ggcctgccgt tgcgcctcct gccataaccg caccgcctat
     3661 accggctgat ccgggtgttc cttcgacttt gccggatgta cctccaatgg cgccaggggg
     3721 tccggtattc gctccgacgg gccctggcgt agacagggcc aagggagaag ccagggaagc
     3781 ggccaaggcg tctggacttg ccggggcagg gccggtgctt tccatcgaca ctgtacaacg
     3841 tgccggactc gaggccaacg gcttggtacg cgccgccagg tcgcaaatag acatggcgga
     3901 ggcgggcgtc gtgggcgcca cggcctaccc caaccctcag cttacattca tgggaggccc
     3961 ccagcatgcg cggattccct ccgcgctgcc ggcgaacata catcggcagt tcactgttgc
     4021 gcagaccatc gaaaacccgt tcttgcgaag cgcccgaatc ggttccgccg aagcggcagt
     4081 ggattcgagc cgcgcgaacc ttgaccaggt gcgtgccgat ctggcggctc aactgcgcgt
     4141 tcgcgcttac gagctattgc tgcggcagga aatagcccgt atggaagcca gtatttatga
     4201 catcatgcag gacgtgcgga ggcgcatcgc gataggagtc agcgtaggag aaacagcacg
     4261 tttcgagttg acaagggcag aggccgaggt gatgaatgcg gccagccgaa aggaagcggc
     4321 tgagctgaat tcggaacgcg cgaggatcgg cctgatgcag cttaccgccg gcgcgttgcc
     4381 ttcgaatttc cagataaagg ggtcgctgta cgaccccgtc accgtgccgg cgctcgaaga
     4441 acttcggcag gaagtgcccg ccgtcaaccc agaggtgttg cgcctcgagg ctgagcagaa
     4501 ccgcgcccgg ttgagaatcg atcaggaacg cgcctcagtg tttccttcag ttcaaatttt
     4561 actcagcaac ttccaggaag ctcagtacac ctccaatctg gccggtatgc acgtaaccat
     4621 accgctgttt tatcgacgtc gcggggagat cgataccgcg gtggcagatt cggcgcgcgt
     4681 gaaggacaca ctggattatc gccgctacga gatcagccag ttgctcgaat cggcctggca
     4741 ggcgttgcaa atcgcgcgcc gcagggtcga gatgttcgag gggggaattg tcaaggaggc
     4801 cgagaccgcg ttccgcattg cccagtctgc ctatgtcctg ggcgagcgtg ggctgatcga
     4861 agtgctcgac actcaacgtg tgctcagggg aatactcgcc gagtccttgc aggctcgctt
     4921 tgacttgcac accgctgctg ctgaaatcga ccggttgcgt gcccactacc ccaaagagca
     4981 gattaccgaa tgaaagcagg tctcattttt tttatttcca ttgtggcatc gcttctgatg
     5041 tcgggatgcg ataacggtga accgccggcc tccccggaaa aaacagtcaa gcaggagaca
     5101 gagaccgcaa gggatgtgaa cagcatcaca attcgtcctg agctcgtgcc ccgcatcaag
     5161 atcgggcatc ccacaatggt cgatcttgcg gataaattac aggttccaag ccgggttgag
     5221 gtggacgagg aaaggctcgt tcgcgtggga tcatacgtta cggggcgaat catcgattta
     5281 tatgtcacgc tcggcgagag cgtcaatgcg ggcgaggctc ttgcacggat taccagtcct
     5341 gagttgaccc aggcacaact tgcctatttg cgcgcgtctt cgcgcacact gctggctcaa
     5401 aagggggctg agcgggcgca tcgcctgctt gaagcggatg tcatccctgt tgctgaagtt
     5461 gaacggcgcg aatcggagct ggaagtcgca cgcgccgagc tcgaggcagc caaggaccaa
     5521 ttgcgcttgc tgggcgtgga cagcagcgtg ctgaaagaat tggcgaggca tgcgcgaatt
     5581 cttccctccg ttaccattaa tgctccgagg aatgggatca tcattggacg gaacgtaatc
     5641 gtgggacaag tggtgcagcc cgctgatcag ctgtttcaaa tagctgatct ttcttccgtg
     5701 tgggtggtgg gtgacgtgcc ggagcagatc gcccgcaacg tcagggtggg gcaacatgtg
     5761 gaaatccacg tgcctgccct tggcgatagg gggttcgacg gactgatcat attcgtggcc
     5821 gacaccgttg atccggctac ccgcacggtc atggtgcgta caatggtgga gaacccccag
     5881 cgcaggctca agccggacat gctggccaat atgcatatca ccgatacgct gcataagagc
     5941 ctggtcgttc ctgagcaggc agtggtgcgc gagggcaatc gtgactatgt cttcctgtcg
     6001 aaaggcaaca acaggtttct cagagtccct gtcgaattgg gtccggaagt tgccgatatg
     6061 cgcccggtaa ttaatggagt caccgtcgat cagagcatcg tggtggaagg cgcctttcac
     6121 ctggacaacg agcgcaagct cgcggaactg gaataagtga tgcttgcttc gatcgtgcgt
     6181 acgatgctca agcagcgcct gctcgtcgta gtggttgcgc tcggcctgtg cgctgctggc
     6241 gtatttgcgg ccaaaaatct taccgtcgac gcattccccg acgtgaccaa catacaggtg
     6301 caggtcgcga cagtagcggt gggacgcagc ccggaggaga tcgagcggct gattaccgtg
     6361 cctgtggaaa tcgcgatgac cggcctgccg gggctggtcg aaatgcgctc cctgaacaag
     6421 agcgggctgt cgctcattac cctggttttt acggaccaga ccgacgtttt cttcgcccgc
     6481 cagctggtca tggaacggct gatcgaggtt tcgccacggc tgatacccgg tattacaccc
     6541 gttctcggac ctgtgtctac cggactcggc gaggtgtatc agtacacgat cgagcatccg
     6601 gatgatggca aacgtcccct tagtgtcgag gagttgaccg aacggcgtac gatccaggat
     6661 tgggtagttc gcccgatgct tcgctcgatc aggggcgttg ccgaagtcaa ttctatcggc
     6721 ggtcatgtca aggaatatca agtattcgtc gatcccaaca aattgcgtca ccatgacctg
     6781 acactggcag cagtcgaccg ggcgatcgcg agcaacaatg ccaatgccag cggcaatatc
     6841 ctgcccttgc acgacgagca atacctgatt cgcggtgtcg ggctgatccg tacgctcgat
     6901 gacattcgca atatcgtgct caaagaggtg gagggcgtgc caatcttcgt gcgcgacgtg
     6961 gccgaggtga agtttggcgg cgaagttcgc gtgggggcga gcatcaagga aggctacacc
     7021 gaggcggtga cggggattgt gatgatgctc cgcggtggaa atgccaagca aatcgtcgag
     7081 caggtcaagg aaaaagtggc cgagatcaac gaacgcaagc tgctgccgca cgggcttcag
     7141 attgttccgt tttatgaccg caccgacctc gttgacgccg cgctggggac cgtttacaaa
     7201 accttgatgg aggcgctcgt cctggttgtc gcggtgcttt tcgtctttct cgggaattta
     7261 cgtacgagcc ttgtcgtatg ttcgacgctc gtcatcactc ctttggtcac cttcatggtg
     7321 atgaatcact atggcatgcc ggcgaatctg atgtcgcttg gaggcctgac gattgctctg
     7381 ggcatgatgg tggacccgac tgtggtcgtg gtggaaaata tttttcagca actgggccag
     7441 gcgcgccgta cgggtgagtc caagtttgac attattgtca gggcgacggc ggaggtggga
     7501 acgccggtaa ttttcggcgt tattgtcatc attcttgttt tccttccact gatgacgctg
     7561 cagggaatgg aagggaagat gtttgcgcca ctcgctatta cgatagccat ctcgcttacg
     7621 gtcgcactga tcgtttcggt gttgctgtca ccggtgctga gtgcttacct tttgaagggg
     7681 gggagcgaaa aggacacaaa aattgtcgct gtcctcaagt cggtatattt gcgggtactc
     7741 aaccgtgcgc tcggctctca gaaagcgact attgtcattg cgctggtctt gctgtcgctc
     7801 gcattcgtcg tatacccctt gctcggcaag tcgttcattc cggtgatgag agaaggctct
     7861 atcacgcctg taatcatccg ggtaccctcg atctcgctcg aggaatcgat cgaactggaa
     7921 gcagaggcga tgagactgat tgcagctata ccgggtgtga aaaccgtggt atcgaagctc
     7981 ggacgtggcg agtcccccgc cgaccccgca tccccgaacg agtcggatcc gattgcagcc
     8041 ttggatctgg aaggttcagg ccgtacgcag caagagatcg aagaggatat acgcaaggct
     8101 ctgacggtat tacctggagt aaacatcgtg ctgtcccagc caatcgagca gcgcgtggac
     8161 gagatggtca ccggagtgcg atcccaggtt gccgtgaaga ttttcgggga tgaccttgag
     8221 gaattgcgca aactgtcgga acaggtagcg cgcatcatcc gatccactcg aggagcacgc
     8281 gacatccgta tcgagcgcct gtcaggccag caggaattga cgatcgatat cgaccggcgc
     8341 gccatcgcgc gtcatggatt aaacgtagcg gatgtgaacg aattgatttc caccgctatt
     8401 gggggcaagg ccgtgaccca ggtgttcgag ggagaacggc gctttacgct tctcctgcgt
     8461 ttccccgagc gtttccgcca tgatatcgag gctatccgcg atctcctgct acgtccgcct
     8521 ggaaatggcg gtaccaatgg cctgcttgct cggggggggc agttggtgcc attgagcgcg
     8581 gtcgccgata tacgggttgt cgacggtccg gctgttgtct cccgagagtt cgccaagcgc
     8641 cgggtcgtag tgggcgcaaa cgtgcacgaa cgcgatctgg gcgggttcgt ggccgaattg
     8701 caggagcgca cggcaaagga ggtaatactg ccccctggat attactttgc atggggcggg
     8761 cagtttgaga acatggagcg tgccatggct acgctttccg tgatcgttcc cgttacactg
     8821 gcggtaatct tcttcctgtt attcatgctg ttcaattcga tcaagctcgc ggtgctcatc
     8881 tttctggctt tgccgttcgc ttccgttggt ggcgtggtcg gattgtacct gaccggcgag
     8941 tatctctcgg taccggcgtc ggtgggtttc atcgcagtct ggggtacatc gatactgaat
     9001 ggggtagtat tgatcgaatt catacggacg ttgcgcttcc aagggctaag cgtgcctgaa
     9061 gcagtgagaa tgggttgcga gcaacgtttt cgtcccgtgc tcatgacagc cgcaacaaca
     9121 gtgctcggtc tggcgccatt cctgatcgca accggccttg gctcggaagt gcagaaaccg
     9181 ctcgcgatcg tggtaatttc tggcatgatc accgcaacga tgatgacgat ggttttaatg
     9241 ccgctgctat accgttggtt tgacgaccag ccgaagcttg aggaagaggc tattgacgat
     9301 gacaccagtc ctcgcctgag tctgcttgga agaatacgtt cgagattcag gaaaaattct
     9361 cccgccgttg ggacgtgatg tccctcggaa tgcgttttcc ccaaaatcgg agcatcgaat
     9421 aacatgaccg attttatctg ctcaaccatg cccttatctc cgtctaccgc aaaagataaa
     9481 gccagaatac tggctgaagc gctgccttat atccggcgtt ttcatggaaa gacgatcgtt
     9541 atcaagtatg gcggcaacgc gatggtcgag gaaaacctca aacaagggtt cgcccgagat
     9601 gttgtattgc ttaaactggt ggggatgaat ccggttatcg tgcatggcgg aggtccacag
     9661 atcgatcacg tgctgaaacg gctcggtaaa cacggagagt ttattcaggg catgcgcgtg
     9721 accgatgcgg aaacaatgaa tgtcgtcgaa atggtgctgg ggggcgtggt caacaaggag
     9781 atcgtcaatc tcatcaatca gcaggggggt cgggcaatcg gcctgacggg caaggacggt
     9841 aattttattt gcgcgaaaaa aatgcgcgtg cgcaacaagg agaaagcggg cgaatggctg
     9901 gatatcgggc aggtcggaga aatcgaaagc atttgtccca ccttgatcga gctgctggaa
     9961 gccaatgatt ttattccggt cattgcgccc attggtgtag gcaggaacgg cgaatcctat
    10021 aacatcaatg cagatctggt cgcaggcgag ctggccaagg tattgtgcgc cgaaaagttg
    10081 atactgctga ccaatacacc tggcgtcctg gataatagcg gcaatttgct gaccggatta
    10141 actgcgcaaa aagtggatga attgattgca gatggtacga tctcgggagg aatgataccc
    10201 aagatcagct cggctctgga tgcggtaaag caaggcgtca agtcctgtca tattgtcgat
    10261 gggagggtgg aggacggatt gcttctggaa gtgcttacag acgaaggtgt tggcacgctg
    10321 ataaaggcga actagccaag gtacaggcga accacgtgga cgcctgtccg cgtcgactag
    10381 atatgcggta gctgatcgag gaattaatgc caggcagatc cggcgataga aaagatcaaa
    10441 tccttcaggt tctggcggaa atgctggaga gccctcgtcc ggaaaaaata acgaccgcgg
    10501 ctctggccgc gaagctgaat gtttcggagg cggcgctgta ccgacatttc gcgagcaagg
    10561 cccaaatgtt cgaggggttg atcgaattca ttgaaaaaac tctatttagc ctgatcaata
    10621 agctcacagc ggaagagaaa agcgggctaa gcggtcccgg gcaggtcgaa ggcatattat
    10681 ccttgttgct cggttttgcc aggaaaaacc ccggtatgac gcgggttttg atcggggacg
    10741 cgctggtgaa cgagaatgac cgcctgcaag cgcgcgtaaa tcaattgcac gaccggctcg
    10801 agacgacctt gaagcaggcg ctgcggtttc aaacaaacga caaggaaaac ccggtcagta
    10861 ttgatgtctc agcacaggca aatatgctga tgtgttatgt tgtgggacga tggcatcagt
    10921 ttgcgaaaag cggcttcaag cgcgatccga tggagtattg ggaagcgcag cgaaaagaag
    10981 tgcttttggg cttggctatc aaagttccgg ctcacaatcg gggttagcgg ccggattggc
    11041 ggcatacctc gcacgagggg tccttattca acttcgttga acgccattcc atcgtcaggc
    11101 cctcaagcat tagcagccgc ccattcaagg tttttccgat ttccagcagt attttcaggg
    11161 tttccgctgc ttgcatacat ccaacaattc ccaccagcgg ggcaaatacc cccatcacgg
    11221 cacagcgcat gtcctgatcg tctccgtttt ccggaaacag gcagtgatag caggggctgt
    11281 tagggtagcg caaatcgaac acagagacct gtccgtcgaa gcgcacgact gcacctgata
    11341 ccagcggttt tttcaaaata gtacaggcac gattaatggc gtagcgcgta gaaaaattgt
    11401 cgctcgcatc caccaccacg ttcgcatgca gggccagttc ttgcagccgg ttttcatcta
    11461 tgcgctcgtc cagtgcaacc acctttactt caggattgat gcgcgcgagc gttttttgag
    11521 cggaacttgc cttgggcgct ccaataaggt ctgtactgtg aacgatttga cgttggaggt
    11581 tggtcaggtc gactgcgtcg ctatcgcaga tggtcagttt gccgactccg ctggcagcaa
    11641 gatacattgc gacgggcgag cccagacccc cggcccctat gatgagcaca tgggatcgaa
    11701 tcagggattc ctgccctgtg ataccaattt gcggtaaaag tatatgacgg ctgtaacgga
    11761 gaagttgctg gtcgttcatc gatggggggc ttgattcgtg ggccagggca agagacaggc
    11821 cgcgctcgat agatgtttca gaaacacgcg gcgtaatgcg ttacaccggg cgcttccata
    11881 gcgctctacg ctttactgct cttcttcatc atttttgcag tgcagctgca agtgagcgca
    11941 agggagcaga tcatggttag cagacagccc aacccgtttt attcattgcg tctcccagtt
    12001 catgaacggg gaagccaggc aaaacaagag ggaaagcagg attaagaagg ttaaataacc
    12061 cgacaaggga agggcgcaat gaatcaagtg ccatcacgcc ccgaaatctt gatcacaatt
    12121 acggaagcgg ctctctaacc tgatttattg cgaggttaca tagtagagaa tagcaagcaa
    12181 agtcacttcg cccgccacgt tcagttcgca accttcgccg cattcttctt tgccgcattt
    12241 tccttgaaat agttcattgc ctgggccagc agcagatcgc cgctggatcc gaactctgca
    12301 ggcggttcgc gcttcttgtt tttctcttca tcaacctctg acttgggtgt cttgatgacg
    12361 ggcctgggcg ctggtgcatt ttttatcgct tcaaggcttt gcttgatttc gtcatctttc
    12421 ccattcgaca aatgccggtc cagatccgct tcacgcaaac gctcggtttc gtctttcgca
    12481 gattcgtcca cgatatcggg agttatcccg cgagcctgga tggactggcc attaggtgtg
    12541 tagtatcgcg ccgtagtgag cttgatagct gtattgttgc ccaggggaag tatggtttgc
    12601 accgaccctt tgccaaaagt ttgagtgccc atgactatgg aacgcttgtg atcctgcaag
    12661 gcgccagcga caatttcgga agctgaagcc gaacccccgt taaccaaggt caccatcggc
    12721 actgtcttga ttcccggagg caagcgcttg aggtaatcat ttttcgtgtc gcgcaggtag
    12781 aattcaggac tggccttgag cttcatcttc gcgtcctcgg tgcggccatc tgtataaacc
    12841 acaagcgaat tggcaggcag gaaggccgcg gaaactgcta cggctccgtt cagcaagccg
    12901 ccaggatcat tgcgcagatc gaggaccagg cctttcatcg gcaccgggct ttccttgaat
    12961 aatttatcga tcgccttggt caggttttca ccggtttgct cctgaaactg cgtaatccgg
    13021 atatatccgt accccggctc gatcatcttt gacttgacgc tctggatctt gatgacggct
    13081 cgcaccagag ggaacgccat gggcttgctc tcgcctttcc gtactatggt cagcgtaatg
    13141 ggagtgttag gcttcccgcg catccgcttg atcgcatcgg tcagcgacag gcccttgacg
    13201 gccgtatcgt ccagcttcac gatcagatcg cccggcttga tacctgcacg gaatgcgggc
    13261 gtatcctcga tgggagaaat gaccttgacg aaaccatctt ccatgctgac ttcgatacca
    13321 aggccgccga actcgccctg cgtgccaatc tgcaattcct tgaatgcatc cgaatccaga
    13381 taggccgagt gcggatcaag cccggtcagc attccattga tcgcttccgt aatcagcttt
    13441 ttatcttcga ccggctcgac gtaatcgctt ttcacacgtc caaatacctc agtaaaggcc
    13501 cgcagctcct caaccggaag agggtgcagg acttcctggg tgtccttgtt cgcgacagca
    13561 gaaaaattga ggctcagcat cacgcctgcg atggcgccga aaatgaccaa gccgaattgc
    13621 cgcatcttgt tacccataga agaatctcca gacggtgtgt tcctcatttt attctgaccc
    13681 agttcaaggg gtcaaacggt ttgccctggt gccgcaattc aaagtataga cctgcgcctt
    13741 tgctcacgcc gccgctgtca ccgaccgtcg cgattgtgtc tccgccggca accgcgtcgc
    13801 cgacttgttt gtgtattgct tcattgttgc cgtaaaggct catgtaattt gctccatgat
    13861 ctacaatcag aagattcccg aatcccctca gccaatcggc aaatacgact cgacctgccg
    13921 caatggcctt gacatccgca ccggtgggcg aacggatgaa taatcctttc cacgtaagac
    13981 caccctcgtg acgtggactc ccaaaacggt ttgcaagttc ccctcgcacc ggcaaactca
    14041 ggcgtccctt caatgccgca aacggattcc cgtcggtcga gccatcaggg acgctgtggt
    14101 tggtaagagg agcggtcgag gacgagggag cagaagggcg aaccgcagga gcagagcgag
    14161 ccgcgcggtt tttccgggca agcatcttcg ccagtttttc caccagccgt gtaaggcgct
    14221 cttcatcctg cttgagcctg gagatttcgc gccgttgctg gtcggcctgt agcgagatgc
    14281 gggtgagcag gcttgcctga tcggtttttt cccgctcgag ctgtttttta taatcagcct
    14341 tctttacctt gatttttgca atttcagcac ttttttcctg gctttcgaga gcaagggaat
    14401 cgagttgctc aaggttgtcc cgaagcgcat cgatggtttc ggatcgagcc ctcgagatgt
    14461 agctgtaata atgcaggtta cgtgcaatct ggtttggttc ctggtgatta agcaggagag
    14521 caagatactc tttttgcccg cctccggcta tgtagcgatg attgagcaat ttttcgagct
    14581 gcgattgttg tatttcaata tctcttgcca gttgctcgga ttgggcgcgt aaccggccca
    14641 gcttttcgac cgccttgtcc tgctcctgcg caagcttgtt cagttgacga ttcgtcgcgc
    14701 ttatcgcccg atccgaatca cgcaacgcat ccgccacctc ggattttgat tcttccgtat
    14761 cagccagttc tttctgcagt gcttcgattc ggctgcgcaa ctgtttgaga tcttctttat
    14821 cgaccttctt aaggtcttcc ttatcaacct tcctgggttc ttccttgccc ccctttgcgg
    14881 cggcatgcag gagatgcggc acaggcagca tcattaccag aagagccacg atgagcttgc
    14941 ggatcatctg caccacgcag cagagttggc tatgtatctg gtgccgacga gcgtgacagt
    15001 cacagatgag gcgcgatctt cccctggttg gcaatcgccc gcatagcctg ttcgacttgc
    15061 gtctggtcgc ccagatagta actccggatc ggcttgaggt catcatcaag ctcatacacc
    15121 aagggaactc ctgtgggaat gttcagctca agcacttcct ccgcggagag attgtcgagg
    15181 tacttgacga gtgccctcag tgaattcccg tgcgctgaaa tgagtacgcg ctgtcccgcc
    15241 cggacctggg gcgcgatggt ttcattccag tagggaagaa accgcgccac cgtatcttcc
    15301 agacactcgg tgagtgggat ctcggaggga tccagatcat ggtagcgcgg atcaaaccct
    15361 ggataacgat cgtcgtcgca agtgagtgcg ggaggcctta tgctgtagct tctccgccag
    15421 atgagcacct gctcttcacc atacttcaaa gctgtctcaa gtttgttcaa accttgcaga
    15481 gcgccgtaat gccgctcgtt cagccgccat gacagattga cgggaatcca catctgatcc
    15541 atttcgtcca ggacaatcca caaagtcctg atggccctct tcaggaccga agtgtaggcg
    15601 acgtcgaacc tgaacccgcc accgcgcaat aatattcccg cgtttctggc ttcttccaga
    15661 cctgttcgcg tgagatccac atccgtccag ccagtgaagc gattctcctt gttccacgtg
    15721 ctttcaccgt gacgcaggag aacgagtttt ttcatatata tttacaaaaa tgtttacaaa
    15781 tgtttacaaa aggatgactt acacaaggat tagtttttac ggcgagaaga ttggattgat
    15841 gtgacgattt taatgcattt cggccctcta cgcagtacgg gaacaggtgg taaaaggctc
    15901 gtttgctttt gagacccttt tttggtcctt aatcgggaac agaacagctg acgagctttt
    15961 aaacaggacg agcttttctt gaatcgacca aagggacagc gcaacggata tgtcctttgc
    16021 ggagcgaagc ggttcctcag aatgcgggtc tctgataaaa tctccggttg agaaaaaaag
    16081 gagatcgtcg tgtcatttgt tcaaaacaat attgccctga tcgtggctgc tgcggcaagc
    16141 ggtattcttc ttttatggca gatttttcgc aagtcccgca atgagatagg aacgatggtg
    16201 gcggttcagc tcatcaatta caaggaggca ttggtgctgg acgtgcggga gcgcagtgaa
    16261 ttcgagtcgg gacatattcc caatgcaaag catattccag cggacaaact ggaagaaagg
    16321 ttgcaggaac ttgaaaaatt caagaacaag cccatcgtaa tcatacatcg agcgggggtt
    16381 agcacgaccg gcaaggcagg ttctatcttg cgaaacaatg ggtttgcgca cgtgcatgac
    16441 ctcgggggcg ggatcgacgc atggaagcag gcaaatctgc cgatcgtcaa gaaataaggc
    16501 attctctatg gccaaggtta tcatgtactc gactgcgttt tgcccgtatt gtgtaatggc
    16561 ggagagaatg ttgcgcgcgc ggggcgttac tgaaattgaa aaaatacggg ttgatctgga
    16621 acctgctcgt cgtagcgaga tgattgaaaa gacgggacgc cggacagtcc ctcaaatctt
    16681 cataggcaat acgcatgtgg gcggttatga cgatcttgcc aaactcgacc gtagtgacgg
    16741 gttgatgaaa ctcctggcgg tctgacgtcc gaaacgtaaa gtttgccgtt gtacgacccc
    16801 ggggtctcta tcgtcgaatc actgattcac tacccatgct tcgagcaaag tccggtttga
    16861 tatctctgtc ggattccaac gcgttgcttt ctgacgtcgc ttgcgccgtt atattgagaa
    16921 taataataaa ccaatcaggc gacgaccttc ctttgctaca ctgactgcct catcgaatca
    16981 tcctcgttga tgcgaaacga gcacaaaaaa tcgaataacc ctttaataga ctgatacgtc
    17041 aacatggaat ccaatacgaa tagccagccg ggcggcgagc agccgcaacc tcttttcagc
    17101 atcgaaaaaa tctacgtcaa ggatctatcc ctggagattc ccaatgcgcc gcgaattttc
    17161 ctggagcggg aaacacccga ggtaaacatc cagttgcaca ccaaggcgga acggatcgac
    17221 gaaggcatgt tcgaatcggt attgactata acggttaccg ccaagatcaa ggataagacg
    17281 atgtttctcg ttgaggtgca gcaagcaggc gttttccaga ttcgcaacgt accggaagcc
    17341 gatatggaac ctgtcctggg aattgcctgc ccaaacgtca tctttcccta tttgcgcgaa
    17401 accgtttccg acatcgtcac ccgtgccggg ttccacgcgg tggttttgaa ccctgtcaat
    17461 ttcgaagcgc tttattacca gggaaagcag cagggcgaaa agagcttatc gacagaggcg
    17521 cctgacggag attcccaaaa ggtaacacac tagaaaaaaa tgaaatatgc tggggcggta
    17581 ctggcgtttc tcggcgatag aacctttcgg cgcgctgttc cggccatgtg tggcttcttg
    17641 ctggcgtttc agatggcggg acctgctttt gccgccctgg aatttctgtc gatcaaggag
    17701 ccggctgtca tcatgtatga tgcgccatcg cttaaagctg acaagctgta tgtcgcgagc
    17761 aagcaccttc cagtggaggc ggtagtaaag gtagagggtt gggtcaaggt tcgcgacagc
    17821 agcggcagcc tcgcttgggt ggaagaaaag cagttaagcg acgcgcggta cgtcatcgtt
    17881 accgcgccat ccgcggaaat tcatcagacc gctaactcaa actcaccgtt ggtcttccgg
    17941 gccctgcaaa atgttgtgct ggaattgctt gagcccgccg cgtcgggttg ggccaaggta
    18001 gagcatcccg acggccagac ggggtatgta agaacgaccc aggtctgggg tacatgagaa
    18061 ttgcagtgct gggcgcgggt gcgtggggca cggcacttgc gatcagcctc agttccaaca
    18121 atagttcttc tcacgaggtt atgctgtgga cgcgagaccc cgagcacttg gccgagcttg
    18181 agtcagacca tatcaatcgc cggtatttcc ccgcgttccc gctccctgac tgcctgggcc
    18241 tgacttcagt tcttggggcg gccatcgaat ttgctgaact tgcccttatc gccgttccca
    18301 cgtatggact gcgcgagacg ctgcgcggaa ttgcggccca tgggaaagat ctgccgctga
    18361 tatggggatg taaaggattc gaatccctgt cggccaagtt accgcaccag gtggtccaag
    18421 aggagtatgg caacgctgca ccctatggcg ttctctctgg gccgagcttc gctcaggaag
    18481 ttgcgcaggg gcttcccacc gccctgaccc tggcatctca tgacgagcaa ttttcacgcg
    18541 taatggcggc cgagttgcgc accccccggc tgcgcgtata ttcatgcaag gatattgttg
    18601 gcgtggagac gggtggagcg atcaagaatg tcatatcgat cgctgctgga atttcagacg
    18661 gcatggggtt cggctacaac gcaagggcag ccttgatcac ccgtggcctt gccgaaatag
    18721 cccgcctcgg cctcaagctc ggcgggcgaa tggaaacttt catgggttta acgggtgctg
    18781 gcgacctgat actgacctgt accggagact tgtcgcgaaa ccgcagggtg ggtctgatgc
    18841 tcgccgcagg ccgttgcttg cctgatatcc tgcatgatct ggggcatact gctgaaggtg
    18901 tgcatactgc ggcagaggtg ggacggctaa gcgagatatt aaaggttgaa atgccaatta
    18961 cgaaggcagt atgcagcatt ctttacgagg gcactcctgc agggctcgcg gtggaagcat
    19021 tgctcaaccg tgaaccgagg aatgaaattt actaaaacag ggatctgagg aacctcttaa
    19081 taagtcatta tgggcgcgac gaccgctttg cgggatggga tgcgcgaaag gcgacgaagc
    19141 gaatggtacg accgcggagg gcgaagcggt ttcttccgca ggaagatgcg accccgtagc
    19201 gggatgaacg cctgccaatt tcatagccgc ggtcgccgca tcccctccgc tgcccgatcc
    19261 cgctttgcga gcccctcgac aacgcttcgg ggcacactgc cttgcgctcc ttggcgtcgt
    19321 agcatggcta cgacctgcgt cgcgcgcctt gtgatcatcc cgattttacc agcaacgcgc
    19381 tccacgagga cttgttcagg ttcccgaggg taccaagcaa ggaacaatcc aaacgagcct
    19441 cggaacgctg tccttgtatc ttgaaatatc ttgtctcgaa atcctgtact ccgtcccgag
    19501 ctatattccg tcccgatcta tattccgtcc tgaaaaccca gttgccgcca ggcctcgtaa
    19561 atcacaactg cgacggtatt ggacagattg aggctccggc ttcccgcaac catgggcacc
    19621 cgtatgcggt attgcgaagg aaggctctcg agcagccaca ttggcaatcc ccgggtctca
    19681 ggcccgaata aaaaaacgtc gccgttcgaa taagcggccc gatcgtatcg ctgcatgcct
    19741 ttggtcgaga ctggaaaaat gcggcgtccc tgcatatgcg ccgcgcaggc ttcccagctt
    19801 tcgtgaacca ttatgctggc gaactcgtga taatcgagac ccgcacgcag caattgcttg
    19861 tcttccaacg cgaatcccag cggcttgacc aggtgaagtc gcgtgcccgt attggcgcac
    19921 aatcttatga tattgcccgt attgggggga atttcaggct ggtagaggac gatatccaac
    19981 ataatcactg cgaagtatag caatcgctca attacgttac accctcggtt ccagagtagc
    20041 gtggggtggg caagggcaat ttcatactgc atttcgagcc aggtctatcc ggatggagct
    20101 ggtaaggcaa ggacggaagc cactcaggct ttggcaaggg gacgcgtctc agggcaaggt
    20161 tctcgcaacc acccagacag acacacgcat tgcgccgcgg ttactcacga gttttgcgag
    20221 ttcattcaca ctcgccccgg tcgtcatcac atcatccagg atgacaacgc gcttccccga
    20281 aaaatcagct tcgcaggtaa aagcgccgcg aatgtttttt tcacgttctt tccatggcaa
    20341 ttccgtttgg gagggcgtat ccctggtgcg ctgacaggca cgagcgaata gcgggatgtg
    20401 agtgcttttc gaaacatacc gggcaatttc cacagcctga ttgaaacctc tctcgcgcaa
    20461 tcgtttagcg tgcagcggca ttgggataat gtaatcaggt aactccgcag ggtttaccgt
    20521 cgaagttatc ctcgcggaca gaaaaccggc cagaacacgt gccattgcca ggttcgagcg
    20581 atatttcagc gactgaagca gagcgtcaac aggaaaagcg tattcgaatg cggcaacagt
    20641 tcggtcaaac gcaggaggtt ttgcaaggca ggctccgcag gtactggccc ctgttaccgg
    20701 gaccgcgcag acactgcaat ggctggaaga gaggtagggc agggtcttgc ggcagggaag
    20761 gcaaaaatca gcactgcctg aatcagcacc aggatcgagg cctgggatga aggccggacc
    20821 ggcaggtgct ccgcaaagca gacaatcgct ccccagcagt gcctgcacaa aacttaatct
    20881 gttgttcaat atcgacagtt aaaaaattga caacggaagc agattccgta atgatttcgc
    20941 atctcaacga tcgacgcgac ccagggctat tgtagcgcct ggaccaaccc gagcaataca
    21001 gcgataaaag gcaaccgcat gagccagttt cattttgatg tgactgataa tagggaaacc
    21061 tgcgggaacg gcacatctga cagcagcgca acggggcctg gcgcttccgc tggatcgttg
    21121 cgctggagta ttcctgcaat cgagtcatta ctggactcgc ctttcaacga tctgatctat
    21181 cgcgcccaag tcgttcatcg tcagcatcac gacgcaaatg ctgttcagct gtcgacgctt
    21241 atttccgtca agacgggcgg ctgccctgag gattgtggct attgcccaca ggctgcacgt
    21301 taccatacca atgtcgagaa tcaggatatg ctgcggcttg acgaagtggt cgcgtcggct
    21361 tcggccgcca aggcgcaggg cgcaacaagg ttttgcatgg gcgcggcctg gcgtggaccg
    21421 aagcagcgcg atatcgaaaa aatgactgaa atgatcggag cggtcaaggc gcttgggctg
    21481 gaaacctgtg tgacgttggg gatgctgaag ccggggcagg cagagcagct ccgcgaagcg
    21541 ggtctcgact attacaacca taatctcgat acatctcccg agttttacgg cgaaatcatc
    21601 acgacccgga catatgagga tcggctcgac accttgggga aagtccgcag cgcggggata
    21661 aatatctgct gcggaggtat cgtcggcatg ggggaatcca ggcgggcccg tgctggattg
    21721 atcgctcagc ttgccaatct cgacccgtac cctgaatccg ttccgatcaa tcacctcgtg
    21781 caggtggaag gaacgccgct acacggcaca gaaacactcg atcccctgga attcgtacga
    21841 acgatcgccg ccgctcgcat aacaatgccc aaagcaatgg taaggttgtc ggcgggcagg
    21901 cgggagatgt cggatgcagt acaggcgctg tgtttcctcg cgggggccaa ctcgatcttt
    21961 tacggagata aactgcttac cacaggtaat cctgaagcag agcgggacaa ggcgctattc
    22021 gggaagctgg ggctgtcttc gatataaggc gatcactcga aaagagtgtt tcgctttatt
    22081 ccccttattc ccttattccc tttattaata ccatgtaagc aatgagcgcg gatctcatcg
    22141 gtggcctcgc cgaacagctg cgcagccgcg agaaaaaaaa tctgttccgc gagcgcgtgg
    22201 cgctggaagg gccgcaggat atccgcgtat cgatagatgg acgtgaacac ctcgcctttt
    22261 gcagcaatga ttacctcggg cttgccaatc atccggaact gatcgtcgcg gcctgcgagg
    22321 gagcgaggcg gtatggagtc ggcgcggggg catcacatct catcagcggg cactccttag
    22381 cacatgacgt attggaagac gcactcgcgc gcttcatcgg gtttcccagg gcactgctgt
    22441 tttcaacagg atatatggca aatgccgggg tggttgctgc gctgatgggc cgcacgggag
    22501 caatattcgg cgacaagctc aaccacgcct cgctcaatga cgccgttctg gtttcaaaag
    22561 cccggttcag ccgttaccgg catggcgacc tggctggact gaagcatcaa atggcagctt
    22621 cgaatgcccg ctccaagctt gtattgaccg atgccgtatt cagcatggat ggcgatctgg
    22681 cgccggtcgc ccgattgctg gagctttgcg aaaaatatga cgcttggctg atgctggatg
    22741 atgcccacgg tttcggcgtc ctgggtgaga agggaaaggg aatcctgggg cacgtggatg
    22801 ttcaatctcc ccg
//
LOCUS       DASSHJ010000055         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_726040, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000055 DASSHJ010000000
VERSION     DASSHJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726040"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFI43_02305"
     CDS             complement(<1..402)
                     /locus_tag="VFI43_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7060991.1"
                     /translation="MKTIKILVITAGTGLLSVAAFIYSGIFNIAADDPHWPFTYKILT
                     VLRDRSIEARSAAISPPDLDDRTRIRLGAGNYDAMCTPCHLSPAIKESELSRGLYPKP
                     PSWREISQVDARQAFWIIKHGIKMSGMPAWGK"
     gene            complement(725..1513)
                     /gene="rsmA"
                     /locus_tag="VFI43_02310"
     CDS             complement(725..1513)
                     /gene="rsmA"
                     /locus_tag="VFI43_02310"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7060992.1"
                     /translation="MTRHIPRKRFGQNFLVDTQVIAAILEAFDPDRDDCLVEIGPGLG
                     ALTWPLLRSVNHLHVVEIDRDIVERLRSEFPEEKLIVHACDALEFDFSTLGKNLRVLG
                     NLPYNISTPFLFHLSQFAPTIRDMHFMLQKEVVARMVAAPSTRDYGRLSVMLQNCFEM
                     EQLFIVPPECFHPAPKVESAFVRMIPRSKPLVESNIQQLFTRIVLAAFAQRRKTLRNT
                     LRDYLHPDDFAELEIDPGQRAENLSVLQFVSIAEYLKDKMQPPQ"
     gene            complement(1583..>2063)
                     /locus_tag="VFI43_02315"
     CDS             complement(1583..>2063)
                     /locus_tag="VFI43_02315"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7060993.1"
                     /translation="TQSLLEQKLKIIHRDLITWFRVPNPRIAVAGLNPHAGESGHLGR
                     EEIDIIIPTLDRLRAEGMDLVGPLPADTLFNPSRLKEYDCIFAMYHDQGLPVLKHASF
                     GTAVNVTLGLPIIRTSVDHGTALELAGTGEADPGSLVAAIEMAAMLAENQKRLTSNG"
BASE COUNT          485 a          527 c          577 g          474 t
ORIGIN      
        1 cttgccccat gcaggcatgc cgctcatctt gatgccgtgc ttgataatcc agaacgcttg
       61 gcgagcgtca acctggctga tctcccgcca ggagggcggt ttggggtaaa gcccgcggct
      121 cagttcggat tccttaatag cgggcgaaag atggcaaggc gtgcacatcg catcgtaatt
      181 ccctgcaccc aatctgatgc gcgtacggtc atcaagatcg ggtgggctta tcgcagcgga
      241 gcgagcttcg atcgaccgat cgcgcaaaac cgtaagtatc ttatatgtga acggccaatg
      301 cggatcatcc gcagctatgt taaaaatgcc ggaataaata aacgcggcaa cgctcaagag
      361 gccagtaccg gcagtgatga cgaggatttt tattgttttc ataagcgttc ctcttcaggc
      421 agactaaatt tcgaacttgt cgctgagttt gacctctctg atcagcagtt agtgctatcg
      481 cagcctggaa aaacaaacct cgctaccgcg agcttctctg gcacggaaaa ggcggggtaa
      541 agaggtggct caaagatgcg caggtttctc tcgcctttac tgacgaggcg ctacgacaag
      601 tcgggacaag gcgattattt ttcgtaaagc ggagaatccg aacaagaaag cgcgggaagg
      661 gcacgagaat gggggaatga tcgagagctt cttccttcgc aggtgctcct gttgacaatc
      721 aggttcactg tggaggctgc attttatctt tcaggtattc agcaatcgag acaaattgca
      781 gcaccgacag gttttcggcc cgctgcccag ggtcgatttc cagctcagca aagtcgtctg
      841 ggtgaagata atctcgaagc gtatttctca gtgtcttccg ccgctgtgca aacgccgcta
      901 aaacaatccg ggtgaacaac tgctgtatgt ttgactctac gagtggcttg ctcctcggga
      961 tcatgcggac gaaggccgac tctaccttgg gcgccgggtg gaagcattcc ggtggcacta
     1021 taaagagttg ttccatttca aagcagttct ggagcatgac cgataatcgc ccgtaatccc
     1081 gggttgaagg agcagccacc atgcgcgcca ccacttcctt ttgcagcatg aagtgcatgt
     1141 cacggatggt gggtgcgaac tgactcagat gaaacaggaa tggagtggaa atgttgtagg
     1201 gaagattacc cagaacgcga agattcttgc ccagcgtgga gaagtcgaat tcaagggcgt
     1261 cgcacgcgtg tacgataagt ttttcctccg gaaattcact acgcaagcgc tcgacaatat
     1321 cccggtcgat ttcaaccacg tgaagatggt tgaccgatcg caacaagggc caggtcagag
     1381 cgcctaatcc cggcccaatc tccacgagac aatcatccct gtctgggtcg aacgcctcca
     1441 ggatcgccgc gattacctga gtgtcaacga gaaagttctg gccgaagcgc tttcgcggga
     1501 tgtgccgcgt catatgctcg gggtcgagtg aagagttgat attctattga tcagacagca
     1561 agccttggga tttcatggcc ctctatccgt ttgacgtcag ccgtttttga ttttccgcaa
     1621 gcattgctgc catttcaatc gctgccacca gactgccggg atcggcctca ccggttcctg
     1681 ccagttccag cgccgtgcca tgatctacag aggtgcgaat gataggcaag cccagggtaa
     1741 cattgacagc tgtaccgaag cttgcatgtt tcagcaccgg aaggccttgg tcgtggtaca
     1801 tcgcgaaaat gcagtcatac tcctttagcc tggaaggatt gaagagcgtg tcggccggca
     1861 gggggccaac cagatccatt ccttcggcgc gcagcctgtc gagggtcggg ataatgatat
     1921 caatctcttc tcttccaaga tgacccgatt ccccggcgtg ggggttgagt ccggcaacgg
     1981 caatgcgtgg attgggaacc cgaaaccatg tgatcaaatc gcggtgaatg atcttgagct
     2041 tttgttccag cagagactgg gtg
//
LOCUS       DASSHJ010000056         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1490312,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000056 DASSHJ010000000
VERSION     DASSHJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490312"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1834)
                     /locus_tag="VFI43_02320"
     CDS             complement(<1..1834)
                     /locus_tag="VFI43_02320"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET7060994.1"
                     /translation="MSHLKYLANDEEINAFDQALEDYRGLRMDADRFTASRLQMGIYG
                     QRQEGVNMVRIKLPGGRLNSAQLNVIADMLEKYARHDVVHITTRQDIQMHYVPLEDTP
                     AVLRHLAIAGLTTREACGNTIRNVTACPLAGICPRQHVDVTKHLDGAVQHFLRNPLTQ
                     QMPRKFKISFSACESDCAQGMMHDMGIVAVRNGDRFGFKVLAGGGLGHKPHEAIVVEE
                     FIEEKDLLLVMEAVISLHNRYSDRTKRAKSRIKFLVDKFGPEGFVEKYREEFVRTKEA
                     LAAQDYPKGEWSPGLDSEVPGMGAPRHVFAQKQSGYYVFPISVPMGNLNVSQLRGLAR
                     IAEEHQLDEVRATQDQNMFLPNIPEAKIEVLRAELARLGLGEPKTGDDVVACPGTSTC
                     RLGITSSTVVGPKLNGGPHDLKIRVSGCHNGCAQPEAGDIGIYGEGKRMHGKLVPHYQ
                     MYFGGNAMAGGALAIKGPSIPSARIETAVERVKTSYASSRSLDESFSSWIRRVGKDQI
                     TILLKDLTEVKPEELDSVLRDHGKDADFKVLQLGGGECAGVSQVKIGSSFFEAAHERN
                     YRDALKFQRKFEDSVKCAEEIARLIGQGVSELLGGQKFEDLAQQS"
     gene            2074..3012
                     /gene="cysB"
                     /locus_tag="VFI43_02325"
     CDS             2074..3012
                     /gene="cysB"
                     /locus_tag="VFI43_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HET7060995.1"
                     /translation="MKLQQLRYLCEVANQGLNLSKAAQILHTSQPGISKQIRLLEDEL
                     GVDIFVRNGKRVVETTPPGRVIIEIAERILRDAKNLKQIGQEFANEATGSLTIATTHT
                     QARYALPTAIKNFTARYPDVKLILRQGSPTQISELVTSGAADIAIATEAIELFAELVM
                     LPCYEWNRCIVVPPRHPLLKLDKLTLEALAQYPIITYDFAFTGRSKINQAFDEKKLIP
                     NVVLTAIDADVIKTYVELGLGVGILAQMAFEPKRDRHLRSIDASHLFEPSTTRIGIGR
                     NSYLRGYVYDFIEIFAPHLTHDVVMTAMKEKEHRSS"
     gene            3068..4927
                     /gene="mutL"
                     /locus_tag="VFI43_02330"
     CDS             3068..4927
                     /gene="mutL"
                     /locus_tag="VFI43_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7060996.1"
                     /translation="MSPIRPSAIKPLSNLLISQIAAGEVVERPASALKEMLENSLDAG
                     STEISINLLQGGMKLIRIADNGQGIPGEDVPLALARHATSKIGTLDDLQQVASLGFRG
                     EALASIAAVSRLTLASRVGEEKHGWKVQAESGRVSEPEPVALARGTTVEMHDIYFNTP
                     ARRKFLKTEATEFSYCEETFKRIALSRPDINFIVQHNGIVRRQLRAGDMQQRISALLG
                     EDFSHTSVFIDESAGDIRLWGLAALPAHARNARDTQYFFVNGRFVRDKLIAHALREAY
                     RDVLHLDRHPAFVLFLEIKPEGVDVNVHPSKIEVRFRDPRALHQFVFHVIDKALAAPA
                     ATRAGVAMAVPAYTASRGIPGKQQEEVASEKEGKPFTYARQSTIPWGQRFEQRPLGVA
                     QPKAAYTTVLFGADPAISESHHVVPEEDTSGHEIPPLGFALGQLRGIYILSQNERGLL
                     IIDMHAAHERLMYEKLKSALDNRSLAMQTLLIPVTFHADSLDIATAAENRAALGELGF
                     EISVISPTVLAVRSIPAALQPADVINLVREVLGDIRQFGSTQAWTGRRNELLATMACH
                     GAVRANRMMTIPEMNALLRDMEATERSGQCNHGRPTWFEISLADLDKMFMRGK"
     gene            5005..>5393
                     /locus_tag="VFI43_02335"
     CDS             5005..>5393
                     /locus_tag="VFI43_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7060997.1"
                     /translation="MKLELLGGISPKKFLREWWQKKPLLIRQAIPGFTGFLNREQLIE
                     LACQDDAHSRVITQAKGKWKVKNGPFRPRAFSRLPETQWTLLVQDVNHFIRPARDLLL
                     RFDFIPYSRLDDLMVSYAPEGGGVGPH"
BASE COUNT         1212 a         1528 c         1437 g         1216 t
ORIGIN      
        1 ccgattgctg cgccagatcc tcgaatttct gtcctccaag caattcggat acgccctggc
       61 cgatcaagcg ggcaatctcc tcggcgcact tgaccgaatc ctcgaatttt cgctgaaact
      121 tgagcgcatc ccggtaatta cgttcatgcg ccgcctcgaa aaagctcgag ccgattttta
      181 cctggctcac gcccgcgcat tcgccgccac ccaattgcag caccttgaaa tccgcgtcct
      241 tgccatgatc ccgtagaacg gagtccagct cttccggttt gacttcggtc aagtctttca
      301 aaagaatagt gatttgatct ttaccgaccc ggcgaatcca cgatgaaaac gattcgtcca
      361 ggctccggct tgatgcgtaa ctcgtcttta cgcgctcgac cgcggtttcg atgcgcgctg
      421 acgggatcga agggcctttg atcgccaacg ctccgcctgc catggcatta ccgccaaagt
      481 acatctggta atgcggcacg agcttgccat gcatccgttt gccttcgccg tagatgccga
      541 tgtcgcccgc ctccggctgc gcacagccgt tatggcatcc cgaaacgcga attttgagat
      601 cgtgcggccc tccgttcaac ttgggcccga ccacggtgct cgaggtgatt cccagccggc
      661 atgtcgaggt tcccgggcat gccacgacgt catctcctgt ttttggctca cccaagccca
      721 gcctcgccag ctccgctctc aggacttcaa tcttggcctc gggtatgttg ggcaggaaca
      781 tgttttgatc ctgcgtcgcc cggacttcat ccagttgatg ttcctcggcg atcctggcaa
      841 ggccccgtag ttggctaacg ttcaaattgc ccatcgggac actgatggga aatacatagt
      901 agccggactg tttctgggcg aaaacatggc ggggtgcgcc catgccgggc acctcgctgt
      961 ccaggccggg actccactcg cctttcgggt agtcctgcgc tgccagggcc tcttttgtgc
     1021 ggacgaattc ttcgcgatac ttctcgacga acccttcggg gccaaattta tcgaccagga
     1081 acttgatccg ggattttgca cgcttggtgc gatcggaata gcggttgtgc agggagatga
     1141 ccgcctccat gacgagcagt agatcctttt cctcaatgaa ttcttccacg acgatcgcct
     1201 cgtgcggctt gtgcccgaga ccacctcccg cgaggacctt gaacccgaag cggtcgccat
     1261 tgcgtactgc gacaattccc atatcgtgca tcatgccttg cgcgcaatcc gattcacagg
     1321 ccgaaaaact gatcttgaat ttgcggggca tctgctgggt caacggattg cgcaaaaaat
     1381 gctgtaccgc gccgtccagg tgcttcgtga cgtcaacgtg ctggcgaggg catatgcccg
     1441 ccagggggca ggcggtaacg ttacggatgg tgtttccgca agcttcacgg gtggtcaatc
     1501 ctgctatcgc cagatggcgg agtacggccg gcgtgtcttc gagcggaaca tagtgcatct
     1561 ggatatcctg gcgcgtcgtg atatgcacga catcatggcg cgcatatttc tccagcatgt
     1621 ctgcaataac gttcagttgc gcggaattca gcctaccccc ggggagcttg atgcgaacca
     1681 tgttgacgcc ttcctgccgc tggccataaa tccccatttg caggcggcta gcggtgaaac
     1741 gatcggcgtc catgcgcaaa cccctataat cctccagcgc ctgatcgaac gcgttgatct
     1801 cttcgtcgtt agcaaggtat tttaaatgac tcatctatat ttctccgcaa attaaggcaa
     1861 catcggccag cacgtccttt taatgcttta ttgcaggcgg cttacctttc gtaactgcac
     1921 ttcgtattac gattgcgcgt tacacgttgc gtttagccgt ccgcagacag ccctatatga
     1981 tgccgcgaaa ttcgcatgat agcaacgcag atatatatct caaaagaata ttatgttaga
     2041 ttgttataat atattcttat ttaaaatccg aagatgaaat tgcaacagtt gcgttatctc
     2101 tgtgaagtcg caaaccaggg tttgaacctg tccaaagccg cccaaattct tcatacatcc
     2161 cagccgggaa tcagcaaaca gatacgcctt ctcgaagatg agctgggagt ggatattttt
     2221 gtgcgtaacg gcaagcgggt ggttgaaacg acgcctccgg gaagagtcat cattgaaatt
     2281 gccgaaagaa ttcttcgtga tgccaaaaac ctgaaacaga ttggacagga atttgcgaac
     2341 gaagccaccg gctcactcac tattgccacc acccacacgc aagcccggta tgcgctgccc
     2401 accgccatca agaatttcac tgcacgctat ccggacgtca agctgatttt acgccagggc
     2461 agccccacac agatatccga gctcgtgaca tcgggagcgg cagatatcgc aatcgctacc
     2521 gaagcaatcg aactgttcgc tgaactggtg atgctccctt gctatgagtg gaaccggtgc
     2581 atcgtagttc cgccgcgtca ccctcttctc aaacttgata aattgacgct ggaagccctc
     2641 gctcaatacc ccatcatcac atatgacttc gcgttcacgg gccgttcaaa gatcaaccag
     2701 gcgttcgacg agaaaaagct cattcccaac gtcgtgctga ccgccatcga tgcggatgtg
     2761 atcaagacct atgttgaact ggggttgggg gtaggtatcc tggctcaaat ggcatttgag
     2821 ccaaagcgag acagacacct tcgatccatc gacgcaagcc acctgttcga gcccagcacg
     2881 acgcggatcg gtatcggacg caacagctac ctgcgcggat atgtttacga ttttatcgag
     2941 atatttgccc cgcacctcac gcatgacgtc gtgatgactg ccatgaagga aaaggagcac
     3001 cgcagctcgt gagtctcgga taattcaggc aaaatgtggt tttggaaaca gcggccaggg
     3061 tccctgtatg agtccgatca gaccaagtgc gattaaaccc ctctccaacc ttctcatcag
     3121 ccagatcgca gcgggagaag tcgtggagcg ccccgcctcg gcactgaagg aaatgctgga
     3181 gaacagcctg gatgctggct caaccgaaat cagcatcaac ctgcttcaag gagggatgaa
     3241 gctgattcgt atcgcggata acggacaggg aattcccggg gaggatgtcc cgctcgcact
     3301 ggcccgccac gcgaccagca agattgggac tctcgacgat ttgcaacagg ttgcgagctt
     3361 ggggtttcga ggtgaagcgc ttgcgagcat tgcggcagtt tcgcggctca ccctcgcgag
     3421 ccgcgtgggg gaggaaaaac acggctggaa agtgcaggcg gaaagtggcc gggtatcaga
     3481 accagaacct gttgcgcttg cccgtggcac gactgtcgaa atgcacgaca tctacttcaa
     3541 cacgcctgct cgccgcaaat tcctgaagac ggaggctacc gaattttcct attgcgagga
     3601 aacattcaaa cggattgctc tctcacgccc cgacatcaac ttcattgtgc agcacaacgg
     3661 aattgtgcgc cgccagttgc gcgcggggga tatgcagcag cgaatttcgg cgcttctggg
     3721 agaggatttc agccacacgt cggtatttat cgatgaaagt gcgggagata tccggctttg
     3781 gggcttagcg gcattgcctg ctcatgcccg gaacgcgcgc gacactcagt atttttttgt
     3841 caatggacgc tttgttcgcg acaagcttat tgcccatgcc ctgcgcgaag cttatcgcga
     3901 cgtgctgcat cttgaccgcc atccggcgtt tgtgctcttt cttgaaatca agccggaagg
     3961 cgtggatgtg aacgtgcatc cgagtaaaat cgaggtcagg ttccgggacc ctcgcgcatt
     4021 gcatcagttt gtctttcacg taatcgacaa ggccctcgcc gcgcctgcgg cgacgagagc
     4081 gggagttgca atggctgtcc cagcttatac ggcaagccgc ggcatcccgg ggaagcaaca
     4141 ggaagaggta gccagtgaaa aagaaggcaa gccttttacc tatgcacgcc aaagcacaat
     4201 cccttggggc caaaggtttg agcagcggcc cctgggcgtt gcccagccga aagctgctta
     4261 tactaccgtg ctattcggtg ctgatcccgc catctcggaa tctcatcacg ttgtgccgga
     4321 ggaggatacc agcgggcatg aaataccacc gctcggattt gccctggggc aacttcgggg
     4381 catttatata ctgtctcaaa atgaacgagg tttgctgatt attgatatgc acgctgcgca
     4441 cgagcggcta atgtacgaaa aactcaagtc agcgctggac aatcgctcgc ttgccatgca
     4501 aacgctgctc attcccgtca catttcacgc ggatagcctc gacatcgcca cggccgcaga
     4561 gaacagggcg gcattgggcg agctgggttt tgaaatatca gtgatatcgc cgaccgtgct
     4621 ggcagtgcgc tcaatacctg cggcgcttca acctgcagac gtaataaacc tggtgcgcga
     4681 agtgcttggc gatatccggc aatttggctc aacgcaggca tggaccggac ggcgcaatga
     4741 gttgcttgca accatggcct gccacggcgc tgtacgggcc aaccgcatga tgacgattcc
     4801 cgaaatgaat gccctgttgc gcgatatgga agcaaccgaa cgctccggtc aatgtaatca
     4861 tggaagaccg acctggttcg agatcagcct ggccgatctt gataagatgt tcatgcgcgg
     4921 aaaataggat atgcccagct tgctccaatt cgccgcacta ctgctttccc ccctcacccc
     4981 gaggccgagc gtaataccta ccatatgaaa ctcgaactgc tgggcgggat ctcgcccaaa
     5041 aaatttcttc gtgaatggtg gcaaaaaaaa cccctgctga ttcgccaagc tatacccggt
     5101 tttaccggtt ttctgaaccg ggagcagctt atcgaactcg cctgccaaga cgatgcacac
     5161 tcgagagtga ttacgcaggc gaaggggaaa tggaaggtca aaaatggacc gttccggcct
     5221 cgtgcctttt ccaggcttcc tgagacgcaa tggactttgc tggtgcagga tgtcaatcat
     5281 ttcattcggc cagcacgcga tttactgctt agattcgatt tcatcccgta ctcccggctg
     5341 gacgatctga tggtcagcta cgcgcctgag gggggcgggg tcggtccgca ttt
//
LOCUS       DASSHJ010000057         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1489623,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000057 DASSHJ010000000
VERSION     DASSHJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489623"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..541)
                     /locus_tag="VFI43_02340"
     CDS             complement(254..541)
                     /locus_tag="VFI43_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7060998.1"
                     /translation="MKLFEEISNPREIRRKLGLNQHDFWSKVGVTQSGGSRYESGRNM
                     PRPVRELVRLVHIEHIDLSKIKKDDLMVAALLKSQNPDLYKSLKKAAKQNK"
     gene            1062..2291
                     /locus_tag="VFI43_02345"
     CDS             1062..2291
                     /locus_tag="VFI43_02345"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7060999.1"
                     /translation="MGLIVQKYGGTSVGSTERIKNVARRIAKFKSREHRVVVVVSAMS
                     GETNRLIALAREVQANPDPRELDAMVSTGEQVSVALLSMALMDQGVKAKSYTGAQVRI
                     LTDSSHTKARILEIDDNRIHADLDEGYVVAVAGFQGVNDRGSITTLGRGGSDTTAVAL
                     AAALHADECQIYTDVDGVYTTDPRIVPEARKLATITFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVKLRVLSSFEEEGEGTLITFEEQEDMERAIISGIAFSRDEAKITVLGVPDRPG
                     IAYQILGPVADANIDVDMIIQNVGHDGMTDFSFTINRNEFTRTMDILRNQVQPHIGAR
                     GVIGGERIAKVSVVGVGMRSHVGIASTMFRALAEEGINIQMISTSEIKISVVVDEKYM
                     ELAVRVLHKVFELDQAA"
     gene            2377..2469
                     /locus_tag="VFI43_02350"
     tRNA            2377..2469
                     /locus_tag="VFI43_02350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2411..2413,aa:Ser,seq:gct)
     gene            2588..>3631
                     /locus_tag="VFI43_02355"
     CDS             2588..>3631
                     /locus_tag="VFI43_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HET7061000.1"
                     /translation="MEFAYPRPQMQRSNWVSLNGTWRFCYDDTRKFAQPSQIKSWPLQ
                     IQVPFPPESKASGIENRGFHSWCWYERDFDCLPLSDRVILRFGAVDYIARVWINGCLA
                     VTHEGGHTPFWADITDMLDPSGKQIVTVQVGDDPHELAKPRGKQDWQLEPHAIWYPRT
                     TGIWQTVWLERVPENYIEKIRWTPRVEIFAIGFEARVIGPEANDLTVDVSLRHGERLL
                     ARDRYQVVDREVDRVIVLSDPGIDDYRNELLWSPERPTLIDATVRLMRGEEVVDEFTS
                     YTAMRSVHILRDRFMLNGRPYMLKMVLDQGYWPDTLLAAPSDEALRRDVELAKAMGFN
                     GVRKHQKIEDPRYL"
BASE COUNT          831 a          928 c          992 g          880 t
ORIGIN      
        1 gaaagaagac gtgccactcc agggagtatt gttatacggt cttgctcgcc catccatgca
       61 gcctgaggca gcaagacttt caaaagtcag tccggaatgg atggatgcgt ttgcatccca
      121 aattcgggcg ctggggctcg aggtaagatc aaatccctga gccgcctccc gtttgattga
      181 ctcctttcga gaacgatttc actctcccgc tgcccggcaa aagcagccat ctttcgttgc
      241 tttcatggac agatcacttg ttctgcttgg ccgccttttt gagactcttg tacagatcgg
      301 gattctgact tttcaggagc gcggcgacca tgagatcgtc cttttttatt ttgctcagat
      361 caatatgctc aatgtgcacc aatcgcacaa gctccctcac agggcgaggc atatttcgcc
      421 cgctctcata acgcgagccc ccgctttggg taacgccgac tttactccag aaatcatgct
      481 ggttcaggcc cagtttgcgc cggatttcac gagggtttga tatttcttca aagagtttca
      541 tgagtcagct cccgagtcgc ttatggttca gtttatggtt gaagttatgt tatagcatta
      601 ttgcacgtac ataaatttaa tttgttttat tattctatgc aagtatccga tacagctaaa
      661 aacggacttt tatcatgtta tccttcggta cgtttttgcc atattgcata ataagtgagt
      721 acttgcaccc gtgcttcttc aattaaaaat tccccctcct ttcacagcat tcacgctgtg
      781 atcggcaaag cactgcgatt gcggtctcca agttaaattt tgcttgagga aaatttatgg
      841 aagagcttta ccatcttccg agatttgtga ttacggataa ggtagaattt acgattagtt
      901 ctcattcgtt acactgcggg gttgtgcagc ccttttcaaa ggcaagagag aggcggctat
      961 tggagaaaga cagccgtaga tgactgccgt cgcgccgttc atcttttgcg cgccagattc
     1021 ggttctcaac gctggagaaa tcaggagaag catcagaaat cgtgggactt attgttcaga
     1081 aatacggcgg cacgtccgtc ggtagtactg agcgcatcaa gaatgtcgct cgtcgaatag
     1141 caaaatttaa atctcgagaa catcgagttg tagtcgtggt ttcagcaatg agcggcgaaa
     1201 ccaatcgcct gattgcgctg gcaagggaag tccaggcaaa ccctgacccg agagaactgg
     1261 atgcaatggt ttcgacgggc gagcaagtat ccgtcgcatt gttatcgatg gcgctgatgg
     1321 atcagggagt caaggcaaaa agctatactg gcgctcaggt tcgtatcctt accgacagct
     1381 cacatacaaa agcccgcata ctcgagatcg atgacaacag gatacacgcg gacctcgacg
     1441 aaggttatgt tgtcgcagtg gccgggtttc agggcgttaa cgaccggggc agcatcacga
     1501 ctttggggcg aggcggttcc gataccaccg ccgttgcgct ggccgccgcg cttcatgccg
     1561 acgaatgtca gatctacacg gatgtggatg gcgtttacac gacggatccg cggatcgtgc
     1621 ctgaagcccg taaacttgcg accatcacct tcgaggaaat gctcgagatg gcaagcctgg
     1681 gctccaaggt gttgcagatc cgctcggtcg agtttgccgg caaatacaag gtgaaactta
     1741 gggtcctctc cagcttcgag gaagaaggcg aaggcacttt aattactttc gaggaacaag
     1801 aagacatgga gcgcgcgatt atttcgggca tcgcgtttag tcgtgacgag gccaagatta
     1861 ccgtgctggg cgtgccggac cgcccgggta tcgcttacca gattctcggg cccgtcgcgg
     1921 acgcgaatat tgatgtcgac atgatcatcc agaatgtcgg ccatgacgga atgaccgatt
     1981 tttcttttac gatcaaccgg aacgagttta cgcgcacgat ggatattctc aggaaccagg
     2041 tccagcccca cattggcgcg cgaggggtga tcggcggcga gaggatagcc aaagtgtctg
     2101 ttgtcggggt gggtatgcgc tctcacgtgg gcattgccag tacaatgttt cgggcgcttg
     2161 ctgaggaagg catcaatatc cagatgattt cgacatcgga aataaagatt tcagttgtcg
     2221 tggatgagaa atacatggaa cttgcagttc gcgtgctgca caaggtattc gaactggatc
     2281 aggcggcctg aacgtttgcc ccgctggatc atcattcatt gcagatatcc atcgtttgac
     2341 cggggaatgg ttaaacgcta ttcttaaagc gtccttggag agatggccga gtggtcgaag
     2401 gcgctcccct gctaagggag tataggctca aaaggcttat cgagggttcg aatccctctc
     2461 tctccgccag atcaatcaac gcggtgattt gccgataaac cctggcgatc tgcgcctatc
     2521 agcccgctcg cttctgagcc cctgatctga tcgctgtaga gtagagtcgt taatcaggag
     2581 aagcccattg gagttcgcct atccgcgccc gcaaatgcaa cgctcgaact gggtctcgct
     2641 taacggaacc tggcgcttct gttacgacga cacccggaaa ttcgcccaac cctcccagat
     2701 aaagtcctgg cctctccaaa tccaggttcc ctttcctcct gagtccaagg caagtggaat
     2761 agagaaccgg ggtttccatt cctggtgctg gtatgagaga gacttcgact gccttcccct
     2821 ttcagaccgc gtcattcttc gcttcggcgc ggtcgactat attgcgcgag tctggatcaa
     2881 cggctgcctg gcggttactc acgagggtgg ccatacgccc ttctgggccg atatcaccga
     2941 tatgctcgat ccatcgggca aacagatagt tacggtccag gtaggggatg acccgcatga
     3001 gcttgccaag ccacgcggca agcaggattg gcaacttgaa ccgcacgcca tctggtatcc
     3061 gagaacgacc ggaatctggc agaccgtgtg gcttgagcgg gtgccggaaa attacattga
     3121 aaagatccgt tggacgcctc gggtcgaaat ttttgccata ggttttgagg cccgcgtcat
     3181 cgggccggag gcgaacgatc tcacagtgga cgtctccttg cggcatggcg aacggctgct
     3241 ggcgcgcgat cgttaccagg tcgtggatcg tgaggtagat cgcgtcatcg tgctatccga
     3301 tcccggaatt gacgattatc gcaatgagct gctgtggagc ccggagcggc ccacgctgat
     3361 tgatgcaaca gtgcggttga tgcgcggcga agaagtcgtg gatgaattca cctcgtatac
     3421 cgccatgcgc tcagtacata tcctgcgcga ccgcttcatg ctcaatgggc ggccttacat
     3481 gctcaagatg gtgctggacc agggctattg gcccgatacg ctgctggctg ccccgagcga
     3541 tgaagccctc aggcgcgacg tcgagttggc caaggccatg gggttcaatg gcgttcgcaa
     3601 gcatcagaaa atcgaagatc cacgttatct t
//
LOCUS       DASSHJ010000058         6630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1160965,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000058 DASSHJ010000000
VERSION     DASSHJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160965"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFI43_02360"
     CDS             complement(<1..259)
                     /locus_tag="VFI43_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET7061001.1"
                     /translation="MNDLEPLENVRIVLSHTSHPGNIGGAARAMKTMGLSTLYLVNPK
                     CFPDKDAERRAAGAGDILDKAQICENLDQALSGTVFAAAVTA"
     gene            301..1122
                     /locus_tag="VFI43_02365"
     CDS             301..1122
                     /locus_tag="VFI43_02365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7061002.1"
                     /translation="MHPMLTIAVKAARRAGGIINRAAQNLDLLHVSKKAHSDFVSEVD
                     GAAEEAIIRTLLEAYPSHSILAEESGARGDPVKSEYQWVIDPLDGTTNFLHGFPHYSV
                     SIALTHKNVLAQAVVYDPFANELFTASRGGGAYLNDHRLRVSKRTQLSECLIGTGFPF
                     REFSHEETYVAMFRDIMPKVAGIRRPGSAALDLAYLAAGRYDGFWEFGLSPWDMAAGC
                     LLITEAGGLLGDMNGNPTHMQSGNLLAGNPKIYEQLLQVFSPHLSKERNIKERPA"
     gene            1179..3251
                     /locus_tag="VFI43_02370"
     CDS             1179..3251
                     /locus_tag="VFI43_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET7061003.1"
                     /translation="METPDFIRFGRAICCDLEQASQREWWLADGLGGYAGGTIAQCLT
                     RCYHGLLIAPADSYQDRSLLFAKADATLYDGDERWSLFTNCWGSGAVEPNGLIHIESF
                     QLEGSIPVWRFAIGDLLVEQCIWMEQEQHSTCIAWRLAHAVDRSIHLDIDLLVNKRSH
                     HEANVSHAFSPAIEGGGHHLAICYGDSRALHFYAEGGQLFARHQWIDDFYLAREHERG
                     LPDREAHLCAAHLRLQLTDKWSGVTATLATGSRPRLAESLRKRQARDASCLTAALVVT
                     PELREAPDWIRQLILSANTFPITKHMPQQKEAGLGTQESHESRNAPYGDSMIAGYPWF
                     GEWGRDTMISLPGLLLTAGRQSAARNILMTYARFVDQGMLPNTLPLDGKNGAPSYNAA
                     DAALWFFEAWRAYLSASHDVGALRDIYPLLEDMVDWHVRGTRHGIGMDKNDGLLHAGE
                     PGLQLTWMDAKVDGCVITPRRGKPVEINALWFNALMCMAQFSHLLGKKADLFTMLAAR
                     TRTGYQRFQKHEGGLYDVLDGPDGDDSSIRPNQILAATLHYSPLPPAAHARIVAETRE
                     HLLTSYGLRSLSPDHSAYHERYEGNVWERDLAYHQGTAWSWLLPHYAMAEYRVTGNAE
                     LAQSCLTPMRDHLLDSGLGSISEIFDGAAPHRARGTPSQAWSVATVLEAWVKLERTRR
                     TPKQKEES"
     gene            3255..6008
                     /locus_tag="VFI43_02375"
     CDS             3255..6008
                     /locus_tag="VFI43_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HET7061004.1"
                     /translation="MSVEAGAEHRRLKNQQHRAENWRLWGPYLAERAWGTVREDYSAG
                     GTAWEYFDHDQSRSRAYRWNEDGLAGISDESQWLCFGLALWNGADAILKERAFGLSGN
                     QGNRGEDVKECYFYRDAVPSHAWLRHLYKYPQSAFPYEQLVHENLRRSREDRPFGLID
                     SGAFALNRYWDVETLYAKASPEELHIRIVATNRGSEHATLWLLPQLWFRNTWSWSDEP
                     CEKPVLYAEPDGKNHSRPIHESERVTPDHEGAPQWAITAEHPLLGNYHLYGREQADLL
                     FTENETNNMQLWGVANAGHYVKDAFHRYLIEGDRAAVNPDQRGTKCAAVYSLTAAPGK
                     SRVIDLVLTAKREAYPFSRHEQVLAERRTEADAFFTALLPQATQQDQRILRQALAGMI
                     WNKQFYHYDVLTWLKGDRMPPPSSRLQGRNHRWTHLKARDVISMPDTWEYPWFAAWDQ
                     AFHCAVLALVDVDFAKDQIELLLRETYLHPNGQVPAYEWAFGDANPPREAMGALKVFR
                     AERVQRGYGDVPFLKRVLHKLLLNHTWWINRKDATGMNVFEGGFLGLDNISVYDRSRP
                     LPAGYILKQADATGWMAMFSLQLTVMALEIATEDSAYEDIAIQCYSQFLAIANTIAGN
                     TGAHVPLWDEVDGFFKDLVVTPDGSTRRIDVFSWVGIVPLFACEVVDARLLAGRIRFN
                     ELLWKHKGGMFNGSEVCACPVTTNDRGEHLLSLVTPSMLVKMLPRLFNENEFLSPHGV
                     RSVSKLHATHQDLGHIPGIGRAVIEYIPGESSSGMFGGNSNWRGPVWMPTNYLLVQAI
                     EKMHRYLGNAFTFPAPCLNGYEINLKYAANLLAERLVDIFRRDECDLIPAFPPESPHQ
                     SDPHWRNLLLFHEYFHGETGQGLGAAHQTGWTGLVANLVMRRYHTDIPEYWRRQGAIT
                     REK"
     gene            6070..>6630
                     /locus_tag="VFI43_02380"
     CDS             6070..>6630
                     /locus_tag="VFI43_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7061005.1"
                     /translation="MESFASDAIIRAMRHQRHSIDQIRIAASARLDPIVRSALGQYMT
                     PWSIARFMASLFSRQTPASVLDAGAGIGSLSVAFLDRFPESRIEAWEIDPILRDYLHD
                     SLAEANASIHRSDFIEDAINNIQFGLGTRFSHAILNPPYKKISSSSRHRGLLSQIGIE
                     TVNLYTAFLALAILLMETKGEIVAIVP"
BASE COUNT         1465 a         1824 c         1861 g         1480 t
ORIGIN      
        1 gagcagtgac agcggctgca aataccgttc cgctcaaagc ttggtcaaga ttctcgcaaa
       61 tctgcgcttt gtccaggatg tcccccgcgc ctgccgcgcg tctttctgca tccttgtcag
      121 gaaaacactt tggattcacc aggtagagcg tactcagacc cattgttttc attgctcgtg
      181 ccgcgccgcc tatattgccc ggatgactgg tatggctcag aacgatgcgg acattttcaa
      241 gcggttcgag gtcattcata ttaaaatccc cagcctgttt aaatttcacg gcttaaaaca
      301 atgcatccca tgcttaccat agcggtgaaa gccgcgagac gcgccggcgg tattatcaat
      361 cgcgctgcac aaaaccttga tttgctgcac gtctccaaaa aagcgcacag cgattttgtc
      421 agcgaagtgg acggcgcagc ggaggaagcg attatcagga cgttgctgga ggcttatccg
      481 agtcattcga ttctagcaga agagagcggt gctcgaggcg acccggtcaa gtctgaatat
      541 caatgggtga tcgacccgct cgatggaacg acaaattttc tccatggatt tccccattac
      601 tccgtctcta ttgcgctcac gcataagaac gtgctggcgc aggcggttgt ctacgatccc
      661 ttcgcaaatg agctgtttac tgccagtcgc ggggggggcg cctatctgaa cgatcatcgt
      721 cttcgcgtaa gcaagcgcac gcagctttcg gaatgcctga tcggaacggg ctttcctttt
      781 cgcgagtttt cgcatgagga aacgtacgtg gcaatgttcc gggatatcat gcccaaggtt
      841 gcaggcatcc gccggcctgg ttccgccgca ctcgatctgg cctacctggc ggcggggcgc
      901 tacgacgggt tttgggaatt cggactttcg ccatgggaca tggcggcagg ctgccttctg
      961 attaccgaag ccggaggatt gctgggagac atgaacggaa atccgacaca catgcaaagc
     1021 ggaaatcttc tcgccggcaa ccccaagatc tatgagcagt tgcttcaagt gttttcgcca
     1081 catttatcaa aagaacgtaa tatcaaagag aggccggcat aagcttccac gggctgcttt
     1141 cccaaggctg tggcagcctg ccgggttgga aataaagtat ggaaacgccc gatttcattc
     1201 gtttcggtcg ggcaatctgc tgcgacctcg agcaagccag ccagcgtgaa tggtggctgg
     1261 cggatggcct tggcggatac gccggcggca ccattgcgca atgtctcacc cgctgctacc
     1321 acgggctcct gattgcgcca gcggattcct accaggatcg aagtctcctg tttgcaaagg
     1381 cggatgcaac gttgtacgac ggcgatgaaa gatggtccct ttttaccaat tgctggggca
     1441 gcggagcggt cgagccaaac ggcttgattc acatcgaatc gttccagctt gaaggcagca
     1501 ttcccgtatg gcgttttgca atcggcgatc tgctcgttga gcaatgcatc tggatggaac
     1561 aggaacagca ttccacctgt attgcctggc gcctggctca cgctgtggac cgttcaatcc
     1621 atcttgacat tgatcttctg gtcaacaaac gcagccacca tgaagcgaac gtttcacatg
     1681 cattcagccc cgcgattgaa ggtggcgggc atcatctcgc catttgctat ggcgacagtc
     1741 gcgcactgca tttttatgcg gagggcggcc agctttttgc aagacatcaa tggatcgatg
     1801 atttttatct cgcgcgcgaa cacgagcgcg gcctgcccga tcgcgaagcc cacttgtgcg
     1861 cggcacactt gcgactacag ttgaccgata aatggagcgg ggtgaccgcc acgctcgcaa
     1921 ctggttcgcg accccgtctt gcagagtcgc tacgcaagcg tcaggcgcgt gacgcctcct
     1981 gcctgacagc agccctcgtg gttacgccag aactgcggga agcaccggac tggatccgtc
     2041 aactcatcct gtcagccaat acttttccca taacaaagca catgccccag cagaaggaag
     2101 ccggactggg cacgcaagag tcccacgagt cgagaaatgc tccatacggc gactcgatga
     2161 tcgccggtta cccctggttc ggcgaatggg ggcgggatac gatgatttca cttccgggct
     2221 tgttgttgac ggcggggcgg cagtctgcgg ccagaaatat tctaatgacc tacgcccgct
     2281 ttgtggatca gggcatgctg ccaaatacct tgccacttga tggaaaaaat ggtgcaccca
     2341 gttataacgc tgccgatgct gcgctttggt tcttcgaagc atggcgtgcg tatctctcgg
     2401 caagccatga cgtgggagcc cttcgggata tctatccctt gctggaggat atggttgact
     2461 ggcatgtgcg tggtactcgc cacggcattg gaatggataa gaatgacggg ctgttgcacg
     2521 ctggggagcc gggcctccag ttgacctgga tggatgccaa ggtcgacggc tgtgtgatca
     2581 ctccgaggcg cgggaagccg gttgaaatca acgcattgtg gttcaatgcg cttatgtgca
     2641 tggcgcaatt cagccatttg cttggcaaga aggcagactt atttacgatg ctcgcagcac
     2701 ggacgcgcac cggataccag cgctttcaaa aacatgaagg cggattatat gacgtacttg
     2761 acggaccgga tggtgacgat tccagtatcc gccccaatca aatccttgct gccacattgc
     2821 actattcccc tcttccaccg gctgcacatg caagaatcgt tgcggaaacg agagaacacc
     2881 tgctgacttc ctacgggctt cgttcgcttt ccccggacca ttcggcttac cacgagcggt
     2941 acgaaggaaa tgtatgggaa cgggacttag cctatcacca gggaacggcc tggagctggc
     3001 tcctccccca ttacgcgatg gccgaatacc gggtgacagg caatgctgaa ctcgcgcaat
     3061 cctgtctcac acccatgcgc gatcatttgc tggattccgg cctcggcagt atctcggaga
     3121 ttttcgacgg tgcggcgccc cacagggcac gcggcacgcc atcccaggcc tggagcgttg
     3181 ccacagtgct ggaagcatgg gtcaagctgg aacgtacccg ccgcacaccc aaacaaaaag
     3241 aggagtcgtg acgcgtgtcc gttgaagccg gcgcggagca caggcggctt aaaaaccagc
     3301 agcatcgcgc agagaactgg cgcttgtggg gtccttatct cgcggagcgg gcctggggca
     3361 cggttcggga agactatagc gcgggtggca ccgcatggga atatttcgac cacgatcaat
     3421 cccgctcccg cgcttatcgc tggaacgaag atggcctcgc gggtatttcg gacgaatcac
     3481 aatggctctg ctttggactg gcgctatgga acggcgcgga tgcaatcctg aaggagcgcg
     3541 cgttcggcct gtcaggcaat cagggcaacc gcggcgagga tgtcaaggaa tgctatttct
     3601 atcgtgatgc cgttccttcc catgcgtggc tccgccatct ttacaaatac ccgcaatcgg
     3661 ccttccccta cgagcagctt gtccatgaaa atttgcgccg ctcgcgcgag gatcgcccct
     3721 tcgggctgat cgatagcgga gctttcgcat tgaatcgcta ctgggacgtc gaaacgttgt
     3781 acgcgaaagc atcacccgag gaactgcaca tccggatcgt tgcaaccaat cgggggtcag
     3841 agcatgcaac cttatggctg ttgccgcaac tctggttccg aaatacatgg tcgtggtccg
     3901 acgaaccgtg cgaaaaacct gttctctatg cggaaccgga cggcaaaaat cactcccgac
     3961 ccattcatga aagcgagcgc gttaccccgg atcacgaggg tgctccccaa tgggcgatca
     4021 ctgcagaaca ccctctgcta gggaattacc acctgtatgg gcgggagcaa gcggaccttc
     4081 tgtttactga aaatgaaacc aataacatgc aattgtgggg ggtggcgaat gctgggcatt
     4141 atgtcaagga tgcatttcac cggtacctga tcgagggcga tcgggcggcg gtgaacccgg
     4201 accagcgcgg cacgaaatgc gcggccgtgt actcgctgac agcggcgccg ggcaaatcgc
     4261 gcgtaattga tctggtatta acggccaaac gggaagcgta tccattttcg aggcatgagc
     4321 aggttcttgc ggagcgccgg accgaagccg atgcgttttt tacagcattg ctgccgcaag
     4381 ccacccagca ggaccaacgt attctccgcc aggcgctcgc gggcatgata tggaacaagc
     4441 agttttatca ctacgacgtg ctcacctggc tgaaaggtga ccgcatgccg ccaccttctt
     4501 cgagactaca gggccgaaac cacagatgga ctcatttgaa ggcccgcgac gtgatttcga
     4561 tgccggatac ctgggaatac ccctggttcg cggcatggga tcaggcgttc cactgcgccg
     4621 tgctggcatt ggtcgatgtc gattttgcca aggatcaaat cgagcttcta ctgcgcgaaa
     4681 cgtacctgca cccaaatggc caggttcctg cttatgaatg ggcgtttggg gatgcgaatc
     4741 ccccccgcga ggcgatgggc gctctcaagg tgttccgggc agaacgggta cagcgcgggt
     4801 acggcgatgt cccatttctc aaacgcgtgc tgcataagct gttgctgaat catacctggt
     4861 ggatcaaccg caaggatgcg acgggcatga acgtgtttga aggcggcttt ctcgggctgg
     4921 acaacatctc ggtttacgac cgctcgcgtc cgttgcccgc cggctatatc ctgaaacagg
     4981 ccgatgcgac cggatggatg gcgatgttct ctctgcaact gaccgtcatg gcgcttgaaa
     5041 tcgcgaccga agactcagcc tacgaggata ttgccatcca gtgctacagc cagtttttgg
     5101 cgattgccaa tacgattgcc ggtaataccg gcgcacacgt gcctctgtgg gacgaggtgg
     5161 atggtttttt caaggacctc gtggtaactc ccgatgggag cacgcgtcgt atcgatgttt
     5221 tttcctgggt cgggatcgtt cccctgtttg cctgcgaagt ggtggatgcg cgcctcctcg
     5281 ccggacgaat ccgtttcaac gaactgctct ggaagcacaa gggaggcatg ttcaatggta
     5341 gcgaagtatg cgcctgtcca gtgacgacca atgatcgggg cgagcattta ctgtcgctcg
     5401 tgacgccatc catgctggtc aaaatgttgc cccggctttt caacgagaac gaattcctct
     5461 cgcctcatgg tgtgcgcagc gtatcaaaac ttcatgccac ccatcaggac ctggggcaca
     5521 taccgggaat cggcagggcc gtcatcgaat atataccggg tgaatcgagc tcagggatgt
     5581 ttggaggcaa ttcgaactgg cggggacccg tttggatgcc cacgaattac ctgctggtcc
     5641 aggccatcga aaagatgcat cgctacctgg gaaatgcctt tacgtttccg gctccgtgcc
     5701 tgaatggcta cgaaatcaat ctgaagtacg cagccaactt gctggcggag cggctcgtgg
     5761 atattttccg tcgtgacgaa tgcgacctga ttcccgcctt cccaccggag agcccgcatc
     5821 agtccgaccc tcactggcgc aacctgctgc tgttccatga atattttcat ggcgaaaccg
     5881 gacaagggct tggggccgcc catcagaccg ggtggacagg gctggtcgcg aacctcgtga
     5941 tgcgccgcta ccacacggat attccggagt attggaggcg gcaaggggct ataacaagag
     6001 aaaagtgacg cctagtattt gtaacttccc ctcctttagt agcgattgaa atagtggctg
     6061 ttccctcgta tggaaagttt tgcaagtgat gcgataataa gagccatgcg ccaccagcga
     6121 cattctattg atcaaattcg aattgcagca tctgcccggc tagatccgat agttagatca
     6181 gccctcgggc aatacatgac gccctggtca attgctcgtt ttatggcttc cctattttct
     6241 cgacaaaccc ctgcatccgt ccttgatgca ggcgctggaa taggatctct gtcagtagca
     6301 tttctagatc gctttcctga aagccggatt gaagcctggg aaatcgatcc tatccttcga
     6361 gactatcttc atgactcgct ggcagaggca aatgcaagca tccaccgcag tgattttatc
     6421 gaagacgcaa ttaataatat tcaattcggt ttgggtacgc ggttcagcca cgcaattcta
     6481 aacccgccct acaagaagat cagcagcagt agtaggcatc gtggcttgtt aagccaaatt
     6541 ggtatcgaaa ctgtcaatct ctatacagct tttttagcct tagcgattct actcatggag
     6601 actaagggcg agattgtagc gattgttcct
//
LOCUS       DASSHJ010000059         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_724483, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000059 DASSHJ010000000
VERSION     DASSHJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724483"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /gene="egtB"
                     /locus_tag="VFI43_02385"
     CDS             <1..1442
                     /gene="egtB"
                     /locus_tag="VFI43_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET7061006.1"
                     /translation="YGIAIFPRSQRLHPGGRVTLRIKIPAAGRVNERIRMQKNAILRI
                     SRLPSSRAPSKTHWIERYGSTRAQTLKLVEPLTPEDHMVQSMPEASPAKWHIAHTTWF
                     FETFILVPHMRGYVPFDERFGFLFNSYYKQLGNHPYRGVRGTFSRPSLDEIYLYRSHV
                     DEAIHKLLDQELSAELLRLIELGLNHEQQHQELIVTDIKHGFWTNPLRPGYQPSSPKR
                     LEDLSRLAPSIWRNFDGGIHEVGHDMHGFAFDNESSRHKTCLQAFSLASRLVTNGEYL
                     AFMDDGGYRRAELWLSDAWDCVCANRWSAPLYWEQSGQSDNAWVQFTCSGMEALQIDE
                     PVCHISFYEADAYARWARARLPTEFEWEIAARSVPDSEKRSANLLEQEIFHPIPARQA
                     NGRSERLLQIYGDTWEWTSSPYTPYPGYRPRPGAEGEYNGKFMCNQIVLRGGSCATPR
                     SHIRSTYRNFFPPDARWQFSGIRLAHDCA"
     gene            1429..2418
                     /gene="egtD"
                     /locus_tag="VFI43_02390"
     CDS             1429..2418
                     /gene="egtD"
                     /locus_tag="VFI43_02390"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET7061007.1"
                     /translation="MTAREQFIARRGAQSPVPVPAGSIAADVFEGLSRAPKMLPPKLF
                     YDAEGSALFEQITKLPEYYPTRTEAAILAENADEICSRLDNDITVSELGAGTATKTRI
                     LLRSLLQHQRSIDYFPLDVSDAALQLAKHEIQRELSTVSVHPQLGDFEDLTFLSQHSP
                     PRLVLYIGSSIGNLEYEEAVALLQDVACHLSRGDRLLLGVDLVKESEVLRAAYDDSAG
                     VTAAFNKNLLVRINRELGGHFELDSFSHISVWNRALSRIEMHLESECRQTVKVDALNV
                     ELDFYQGERIHTENSYKYTIESLEELLGRAGFALEHTWTDSRGWFAVSLGMVI"
BASE COUNT          618 a          717 c          673 g          595 t
ORIGIN      
        1 gatatggcat tgcaattttc cctcgcagcc agcgcttgca cccaggcggg cgagttactt
       61 tacgaataaa gataccggcg gcaggccgcg ttaacgaaag gataaggatg caaaaaaacg
      121 caattctacg tatatctcgc cttccgtcca gtcgagcccc atcgaagacg cattggatcg
      181 agcgctatgg cagtactcgc gcccaaacct tgaagctcgt cgagccactt acgccggaag
      241 atcacatggt gcaatccatg cctgaggcga gtccggcaaa atggcatatc gcccatacca
      301 cttggttctt cgagacattt attctcgtac cgcacatgcg gggttatgtt ccattcgacg
      361 aacggtttgg tttccttttc aattcatatt acaaacaact gggcaatcat ccttatcggg
      421 gcgtgcgggg cacattttcg cgcccgtctc tcgatgagat ttatttatac aggtctcacg
      481 tagacgaggc gatacataag cttttagacc aggagctttc cgccgagctt ttgcggttga
      541 tagagctcgg tcttaatcac gagcaacagc atcaggaatt aatcgttacc gacatcaagc
      601 acggcttctg gaccaaccca ttgagacccg gctaccagcc cagcagtcct aaacgcctcg
      661 aagatctgtc tcgcctagcc ccctccatat ggcgcaattt tgatgggggg atccatgaag
      721 ttggccacga tatgcacggc ttcgcattcg ataacgaatc atccaggcac aagacatgtc
      781 tgcaagcctt ttctctggcg tccaggctcg ttaccaatgg tgaatacctg gcattcatgg
      841 atgacggagg gtaccgccgg gccgagttgt ggttatccga tgcgtgggac tgcgtctgtg
      901 ccaatcgctg gagtgctcct ctttactggg aacagtcagg gcaatccgat aacgcgtggg
      961 ttcaatttac atgcagcggc atggaagctt tgcagatcga cgagcctgtc tgccacataa
     1021 gcttttatga agcggatgct tacgcccgat gggcccgggc gcgtcttcct accgagttcg
     1081 agtgggaaat cgctgcgcgg agcgtccctg attccgagaa aagatcggca aaccttctgg
     1141 agcaagagat ttttcatccg attccggccc gtcaggcaaa cggtcgatct gaaaggctcc
     1201 tgcaaatcta tggggatacc tgggagtgga catcaagccc ctacacccca tatccgggct
     1261 atcggccacg tccgggagct gaaggcgaat acaatggcaa gttcatgtgc aatcagatag
     1321 tgttgcgcgg aggctcctgc gcaacacccc gatctcatat ccgcagtact taccgcaatt
     1381 tttttcctcc agacgcccgc tggcagtttt cgggcattag gttggcccat gactgcgcgt
     1441 gaacagttca ttgcccggcg tggggcgcaa tctccagtgc cagtcccggc gggatcgatc
     1501 gcagccgatg ttttcgaggg cctctcccgg gcgcctaaaa tgctgcctcc gaagttgttc
     1561 tatgatgcgg aaggttcagc attatttgag cagatcacaa aattgcccga gtattatccg
     1621 acacgaacgg aagcagccat attggcggaa aatgcagatg aaatatgctc gcggcttgat
     1681 aatgacatca ccgtgagcga actgggtgcg ggaaccgcaa caaaaacccg gattttgctg
     1741 cgctcattgc tgcaacacca gcggagcatc gattattttc ccctggatgt ctcagatgcc
     1801 gcactgcagc ttgcaaaaca cgagattcaa cgggaactct caactgtcag tgtgcatccc
     1861 caacttggag acttcgaaga tctgacgttt ctcagccagc actcaccgcc gcgcctggta
     1921 ctttatatcg gctcaagcat tggcaacctg gaatatgaag aagccgttgc cctgctccag
     1981 gatgtcgcat gccacctgtc acgcggcgac cggttgctgc tcggcgtgga cctcgtgaag
     2041 gaaagcgaag tattgcgggc agcctatgat gacagtgctg gcgttaccgc cgcattcaac
     2101 aaaaatctgc tggtacgtat caaccgcgag ctgggcggcc atttcgaact tgactctttt
     2161 tcgcacatat cggtctggaa tcgcgcctta tccaggattg aaatgcacct ggagagcgag
     2221 tgccggcaaa ccgtaaaggt tgacgcgcta aatgtggagc tggacttcta tcaaggtgaa
     2281 cgcattcaca ccgaaaacag ctacaagtac accattgagt ctcttgagga actccttggg
     2341 cgcgccggct tcgccttgga acatacctgg accgattccc gaggctggtt tgcggtcagc
     2401 ctcggcatgg taatataggc agcactgtag aatgcgggaa tacctggaat attccggccc
     2461 tcctcatact accgaataat tcctctcgcg ctaaagtagc aacggaagcg gaaaacgtcc
     2521 tgtcacaggt ttcatccgca agctcatcgc gcttgcaccc atcccctatt tagacgaaat
     2581 gccctgctac agaaaaacaa tac
//
LOCUS       DASSHJ010000060         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_287083, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000060 DASSHJ010000000
VERSION     DASSHJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287083"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFI43_02395"
     CDS             complement(<1..631)
                     /locus_tag="VFI43_02395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7061008.1"
                     /translation="MAGRKFDSSRRNWLRTMLAAAGVIAADGLVGATSWSSIFTLVTF
                     GDSILDCARYNEFGVHPGQLIVRNDDDLFPEFEHRDLFSLGLAHLDHRAVDGATVSSL
                     PRQAQGLLVEGPAVALVAIGGNDLIEGLAADRGGEGIQRFKQELDAFLHRLPIRPILL
                     GNVYDPTFGDDSRNFLTVDASIARPNFNRVNAVIKELAPRYGQFVDLHQH"
     gene            complement(677..934)
                     /locus_tag="VFI43_02400"
     CDS             complement(677..934)
                     /locus_tag="VFI43_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7061009.1"
                     /translation="MTYDSDVSRMKWVIGGAAVGALTMFLLDPDRGNRRRAMAKDKVH
                     SAVTKTRKEIDAKSRDLANRAKGLGHEVTHIFSSPQGSSPN"
     gene            1408..2037
                     /locus_tag="VFI43_02405"
     CDS             1408..2037
                     /locus_tag="VFI43_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7061010.1"
                     /translation="MSSPGPATHFSMIRGFQLADFFTLGNAACGVAAVFFAMLYIESR
                     SSAYVLGSAGMMAAAFVFDMLDGRIARWRHKHSALGRELDSLADVLSFGIAPAALGFA
                     AGLRGGWDWLALIYFVCCGVSRLARFNITAETLSVGSDKVAYFEGTPIPTSIVLAGVL
                     AWAGWADRLGNNLYGGLWSIGPWELHPLALLFVISGTMMISKTLRIPKI"
     gene            2062..3303
                     /locus_tag="VFI43_02410"
     CDS             2062..3303
                     /locus_tag="VFI43_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7061011.1"
                     /translation="MASPYENVSAAVFCDFENVALGVRDAKYEKFDIKRVLERLLLKG
                     SIVVKKAYCDWDRYKGFKDTMHEANFELIEIPHIRQSGKNSADIRLVVDALDLCYTKS
                     HVNTFVIISGDSDFSPLVSKLRENAKQVIGVGVKKSTSDLLIANCDEFIFYDDLVRES
                     QRAAAKRPAKEQQAAAKQPSGEEQRRKEELEARKSEAIEIAVDTFDALVSERGDSGKI
                     WASTLKQAIKRRKPGFNESYYGFRAFGNLLEEAQARGLLELGRDEKSGAYVYRPGSAK
                     PGGEPIVEQAQSSPPPPEDSATVAGDGSAGKPARQGRRTRKPSTAKKTETGPSVPAQP
                     QVHENQGAVESETNTPEPPQIAVSKPQVPASEPEREAATVEAPAQLASEEMPADKKPA
                     RRKSPTGSRRPRKPKTLPDAA"
     gene            complement(3512..3799)
                     /locus_tag="VFI43_02415"
     CDS             complement(3512..3799)
                     /locus_tag="VFI43_02415"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HET7061012.1"
                     /translation="MNDKNVFISAVASLLVVGLVGGCGRSQAPHEVRPVEWYEANPAE
                     RAAKLEECKSSTQKLDATPDCINASRAENNTKATTKWGEGRGEVRTVPTIP"
     gene            4386..>5105
                     /locus_tag="VFI43_02420"
     CDS             4386..>5105
                     /locus_tag="VFI43_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061013.1"
                     /translation="MPTGLSTPALGFSAAYAINNAGQIAGSSSLSPQNPLVAHQASLW
                     NGAKVVDLGPPGSEQPSVGNAVNDSGQVAGWSENPRDAGYQATVWNGPKATALDTLAG
                     WSFSSAQAINDSGQAAGYSTLRVGEDPSTPTTYRATLWNGTVATDLGTLGGQYSFANA
                     INETGEVAGWAQLAEPGAYHAALWSGSAVSDLGTLGGTNSAANAINSEGVAVGWAVTA
                     GDIQHAALWDGATAADLNSLLD"
BASE COUNT         1091 a         1501 c         1412 g         1101 t
ORIGIN      
        1 agtgttgatg cagatccacg aattgcccat accggggagc gagttctttt atcacggcgt
       61 tcacccggtt aaagttggga cgggcaatgc tcgcatcgac ggtcagaaaa ttgcgcgaat
      121 cgtcgccaaa cgtggggtca taaacgttgc cgagcaaaat cggccggatg ggtagcctat
      181 gcaggaaagc gtccagctct tgtttgaatc gctggatgcc ttcaccccct cgatcggccg
      241 caagcccctc gataaggtca ttgcctccaa tcgctaccag ggccacggct ggcccctcca
      301 ccagaagccc ttgtgcttgc cggggaaggc tgctgactgt ggcgccgtcc accgcgcggt
      361 gatcgaggtg tgccaggccg agtgaaaaca ggtcacggtg ctcgaattcg gggaacaggt
      421 catcatcgtt gcgaacgatg agctggcccg ggtggacgcc aaattcgtta tagcgggcgc
      481 agtcgaggat cgaatcgcca aacgtaacga gtgtaaatat gctgctccag ctcgtggccc
      541 ctactaaccc atcggcggcg atcaccccag ctgcagcgag catcgttcgc agccagttac
      601 gccggctgct atcgaacttc cgtcctgcca tccaggccaa tcgccactgc tgtctaaaat
      661 agttttaaag ggaatgtcag ttggggctgg aaccctgcgg ggaggagaag atatgggtta
      721 cttcatgtcc cagccccttt gcccggttgg caagatcgcg ggattttgca tcgatttcct
      781 tgcgtgtctt cgtcaccgcg ctgtgcactt tgtccttagc catggcccgg cgcctgtttc
      841 ccctgtccgg atcgagcaaa aacatcgtga gagcgccgac tgccgcaccg ccaatcaccc
      901 atttcatgcg agacacatct gagtcataag tcatcttcgt tctccttttc tcgacaaaaa
      961 aggtcattat tctcttgtca gcttcgccta tcggtgcgtt aatccacata taaaccttta
     1021 gccatacctt tgatgctgga ttctcgccgc gcccgcttgc gtatcgacca catccaattg
     1081 cactctcgcc agaatctgat cttccacata acgtcattca ggagctgatc tccacacaac
     1141 gtcatttccg tgaaaacgga aatccattgc tttaacctta ccgtttcgtc ttgtagatcc
     1201 gactactgct ttcgcgagtt catcacactc aacggacgtt tgttcatccg gaaagggcgc
     1261 cggcaggatc ggttgccaag gtgtaacgat ctttttatcg ggaggccctg ctcccttttt
     1321 aagcactcgc cgccttggct ggctacaata aaaatgagta aaagcctatc aatatgaatg
     1381 aagccacagc caaataagct tgaaccgatg tcctcgcccg gtcccgcgac gcatttctcg
     1441 atgatacgcg gcttccagct cgcggatttc ttcaccctcg gcaatgctgc ctgcggtgta
     1501 gccgccgtct ttttcgcaat gctgtatatt gaaagccgtt cttcggctta tgttctcggc
     1561 tcggcaggga tgatggcagc ggctttcgtc tttgacatgc tcgatgggcg cattgcacgt
     1621 tggcgccata agcactcggc cctcgggagg gaacttgatt cgctcgccga cgtgctctca
     1681 ttcggcattg cgcctgccgc cctcggtttt gctgccggtc ttcgcggcgg ttgggactgg
     1741 ctcgcgctga tctactttgt ctgctgcgga gtgagccgct tggcgcggtt caacataaca
     1801 gcggaaacgc tttcggtagg gtctgacaag gtggcgtact tcgaaggcac gccgatcccg
     1861 acaagcatcg tgctcgcggg tgtgctcgct tgggctggct gggcggatcg cctgggaaat
     1921 aatctctacg ggggtctatg gtccatcggc ccctgggaac tgcatcccct ggcgctcctg
     1981 ttcgtcattt ccgggacgat gatgatcagc aagacattgc gcattccaaa aatctaacga
     2041 aaaacaggag aaacgtatca catggcttca ccctacgaaa atgtcagcgc cgcagtattc
     2101 tgcgacttcg agaacgtcgc gctcggcgtt cgcgatgcga aatatgaaaa attcgacatc
     2161 aagcgcgtgc tcgagcgtct gctgctcaag gggagcattg tcgtcaaaaa ggcgtattgc
     2221 gattgggatc gttacaaggg attcaaggac acgatgcatg aggccaattt cgaactgatc
     2281 gaaattcccc atatccgcca gtccggcaag aactcagccg acatccgcct ggtcgtcgat
     2341 gctctcgacc tttgctatac caaatcgcat gtcaatacgt ttgtcattat cagcggtgac
     2401 tccgattttt cacccctggt ttcaaagcta cgagaaaacg ccaagcaggt gatcggcgta
     2461 ggagtcaaga aatcgacctc cgacctgctg attgccaact gcgatgaatt catcttttat
     2521 gacgatctgg tgcgcgagag ccagcgggcg gcggccaaac gcccggcgaa ggaacagcag
     2581 gctgcagcga agcagccatc gggcgaggaa cagcgccgca aggaagagct ggaggcgcga
     2641 aagagcgagg cgatcgaaat tgcggtcgac acctttgacg cattggtatc cgagcgtggc
     2701 gatagcggca agatctgggc ctccacattg aaacaggcaa tcaagcggcg caagccgggc
     2761 tttaacgagt cttactatgg ctttcgtgcc tttggcaatc ttctcgaaga agcgcaggcg
     2821 cgcggtctgc ttgaactggg gcgcgatgaa aaatcgggcg cttacgttta ccgcccgggt
     2881 agcgcaaagc cgggcggcga accgattgtg gaacaggcgc agtcttcacc tcctccaccc
     2941 gaagactccg ccacggtcgc aggtgacggt tcagccggaa agccagcacg ccagggtcgt
     3001 cgaacccgca agccctcaac cgcgaagaaa actgaaaccg gaccatctgt accggctcag
     3061 ccacaagtac atgagaatca gggagcggtc gaaagcgaga ccaatacacc cgagccaccc
     3121 cagatcgcgg tgtcaaagcc tcaagtacct gcatctgaac cagagagaga agcagcgact
     3181 gtcgaagccc cggcccaact ggcatcagaa gaaatgccgg cagataaaaa accggcgcgg
     3241 cgaaaatccc cgacgggaag ccgccgtcct cgcaaaccca aaaccttacc ggacgccgct
     3301 taagagcgac cgcggaggga ggaacagtat cttgcccgca ggaagatact gcctgagaaa
     3361 attacctggt atgacgaagt tcagcgagta cagccgtggc atccccttcg ctggcgatcc
     3421 gcttcgcggg tccctcgcct acgctacgag gcggccaccc ctaccgggat accggcacgt
     3481 cgataaacga tgcgtcgaga taccccctac attaagggat ggtcggcact gtcctgacct
     3541 cgccgcgccc ctcaccccac ttggtagtcg ctttcgtgtt gttttctgcg cggctcgcgt
     3601 tgatgcagtc aggcgtagcg tcgagtttct gcgtgctgga tttacattct tcgagttttg
     3661 ccgcccgttc agcgggattg gcctcgtacc attcaacagg ccgcacttcg tggggggcct
     3721 gtgaccttcc acagcctcca accaggccga ccaccaagag gcttgccacg gcggagataa
     3781 acacgttttt atcgttcatg accgaactct tcaggaagac gaattgagtc tgttcacccg
     3841 gagagtttgc tccatcatgt ttttgctatg cttctctcgg gcaacgtcct ggtttgagca
     3901 ttgcaaacat tccccgtctc ctctgtgcgg ttgcgcactg attcattgcc tatcaaaaac
     3961 ctataaatcg gcgtacggga tgctgtctcc tgagagtttg cgaaaatcca gggacaaaat
     4021 cagatcctcg tcacctgtca catgagccat gccgcttgca ctgaagcttt gaagtgaacg
     4081 tttcgcggta ttgcgcaatc ctcatcacgg agtctgaaat gcatacagga tcgtgctgct
     4141 ctgcctcgcc ctcctcagtt gttctagccg ctgccctgtt tgccgccctg tccgccggcc
     4201 cgctctctag tacttcgcga gccagcagcc ctccggccag cctctcctac agattcacaa
     4261 acctcgcccc gctgggagga gcttttagtt ccgcccgcgc tatcaataat aacagtctca
     4321 tagcagggtt ttcctctttc cccggagggg gttaccatga tcaggcgaca atttgggatg
     4381 gctccgtgcc caccggtctc agcacaccgg cgcttggctt cagcgcggcc tatgccatca
     4441 ataatgccgg gcagatagca ggcagttcat ccctttcccc gcaaaaccct ttggtcgcac
     4501 atcaggcatc actgtggaat ggcgccaagg tggtggatct cggcccgcct ggatcggagc
     4561 agccgagcgt aggtaacgcg gtgaacgatt ccgggcaagt ggcaggatgg tccgaaaatc
     4621 cccgcgatgc ggggtatcag gctaccgtgt ggaatggccc caaggccacc gctctggaca
     4681 cgctggccgg ttggtctttc agttctgccc aggccatcaa cgactccggt caagccgcgg
     4741 ggtattctac gctccgggtt ggcgaagacc cttccactcc gaccacatac cgtgcgacgc
     4801 tctggaacgg tactgtggca acggacctgg gcacattggg ggggcagtac agcttcgcga
     4861 acgcgattaa cgaaaccggg gaggtcgcgg ggtgggccca gttagctgag ccgggcgctt
     4921 accacgcagc cctctggagc ggatcggcag tcagtgacct ggggacactt ggcgggacaa
     4981 acagcgcggc caacgccatc aatagcgaag gcgtggctgt cgggtgggcc gttacggccg
     5041 gtgacattca gcatgccgca ctctgggatg gagcaacggc ggcagacctc aacagtttgc
     5101 tggat
//
LOCUS       DASSHJ010000061         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_722859, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000061 DASSHJ010000000
VERSION     DASSHJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722859"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(543..1883)
                     /gene="msrA"
                     /locus_tag="VFI43_02425"
     CDS             complement(543..1883)
                     /gene="msrA"
                     /locus_tag="VFI43_02425"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7061014.1"
                     /translation="MKAVIAMAAGLSVIMGVYAMEKTAASREATDYVVLGMGCFWGAE
                     KRMGEIPGVVDVESGYAGGNLPNVGYRDILNHEKALRAGKTAARNHAEVVKVVFDPAK
                     VTLETVLAKFWENHDPTQGNRQGNDVGSNYRSAIYYHNENQKAAALATQAVYQKALDA
                     ADYGKITTEILPLKNYITAEEYHQNYLKKNPDGYCGLGGTGVKYPDSAKKVAQAGRSP
                     VSSETSSASSASKITSEPLKGSDLNATQQLVAFEAEHCEFCERFEKDIMSHWQAEVPI
                     TRTLNSNVPSGWTLEKPLFAAPTIVLFKNGKEAARYTGYNGEKSNFWKWLGFQMLSPE
                     QQKIAFKQGTEPPFTASNLDEKRPGRFVDPITGATLFYSNTKFESGTGWPSFFDPVEG
                     AVTLHEDNSHGMRRVEVRSASSGIHLGHLFNDGPPPSHKRYCINGNVLKFVADR"
     gene            2663..2956
                     /locus_tag="VFI43_02430"
     CDS             2663..2956
                     /locus_tag="VFI43_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061015.1"
                     /translation="MSARNIRPFVDHDELARRYENRAGKLLEIAAEHEKRLQYYEDKS
                     YLYGRHQQDSQAHTIALVQKYKRAAEKATESAFHHRMVSQLAKRDYIASGASH"
     gene            3077..>3849
                     /locus_tag="VFI43_02435"
     CDS             3077..>3849
                     /locus_tag="VFI43_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7061016.1"
                     /translation="MPLHIETPLIESPSISHKVGQSIWLKMESAQPSGSFKARGIGYA
                     CEEYLKRGAKRFIASSGGNAGLAVAFAGRSLSVPVVIVVPKTASKRAVNLMGLENAEV
                     IVHGESLDEAHRFALSLIQPADAYIHPFDDPLLWKGHASLIDEIFRAGLKPDAIVLSV
                     GGGGLLCGVVEGLKQNGMGDVPIIAVETEGADSYGKSLNAGFLVELPNISSIATTLGA
                     RIVCEQALAYSKTHHIKSVVLPDKIAVSASLQFLQDHGIV"
BASE COUNT          921 a          965 c          989 g          974 t
ORIGIN      
        1 ccaataccaa cataccttta gcggcttcga aggccgccac ccaccgaaga ttggcgcgct
       61 tgtatctcat tcttttacgg ttctttcgtt gatggcccgc caactgcctt ttttcctgct
      121 cccttttcgc tagctggaaa atgtacgggc agagccttga gttattgatg cgtatcatcc
      181 gtgatttttt ttggaattaa tgacaagttt cagagcgagg ggagagccac taatatagcc
      241 aaaaagaaat atctcgccgg gatttttatg tagaggcgcc ccccggaccc cttcaagtgc
      301 ggagaaaagc tatccgggac aagaaagttt aaggaagagg caagggaaga aattgtcgag
      361 cgggcttagg tgtttagaag agagatatag ggtattaaag cctcgtagcg tgatgctgaa
      421 aaagttgcat ccggataaga aacgagcgcg ggtaataaaa tactcgcgat tgccgccact
      481 ccggtccttc cgcgtacacg gtataaaaga ctcgaccgga gggtcaatgc atagaggccg
      541 atttatcgat ccgcgacgaa cttcaggaca tttccgttga tgcaatagcg cttgtggctg
      601 ggtggcggcc cgtcgttgaa tagatggccg agatggatgc cggaactcgc gctacgcact
      661 tcgacccgcc tcatgccgtg cgagttatct tcatgcaggg tgaccgcgcc ttccacggga
      721 tcgaagaaac tgggccagcc cgttccgctt tcaaatttgg tattgctgta aaagagcgtc
      781 gcgcccgtga tagggtcaac aaatcttccg ggccgctttt catccagatt ggatgcggta
      841 aaaggaggct cggtgccttg cttgaaggcg attttctgtt gttcggggga cagcatctga
      901 aagccgagcc atttccagaa attggatttc tcgccattgt atccggtgta gcgggcggcc
      961 tccttgccgt tcttgaacaa cacgatggtg ggtgccgcga acaggggttt ttccaacgtc
     1021 cagccgctgg gcacattcga gttcaatgtc ctggtaattg gcacttcagc ttgccaatgg
     1081 ctcatgatgt ccttttcgaa gcgctcgcaa aattcgcaat gctcggcctc gaatgccacc
     1141 aattgctggg tggcgttcaa atcgcttccc ttgaggggct cggatgttat tttcgacgct
     1201 gacgaggccg aagaggtctc cgaggagacg ggcgacctgc ccgcctgcgc aaccttcttc
     1261 gctgaatcag gatatttcac cccggtgccg cccaggccac aataaccgtc cgggttcttt
     1321 ttcagatagt tctggtggta ttcctcggcg gtgatgtaat tcttgagcgg caggatttca
     1381 gtcgtgatct tgccatagtc agcggcatca agcgccttct gataaaccgc ctgggttgcc
     1441 agtgccgcag ccttctggtt ctcattatgg taatagatgg cgctgcgata attgctgcct
     1501 acatcattcc cctgccggtt accttgagta gggtcatggt tctcccagaa ttttgccagc
     1561 actgtttcca gcgttacctt ggctggatcg aaaaccacct tcaccacttc cgcatgattg
     1621 cgcgcagcgg ttttaccggc tcgaagcgct ttttcatgat tcagaatgtc ccggtaaccc
     1681 acgttgggaa gattgccacc tgcgtagccg ctttccacgt cgactacgcc gggaatttct
     1741 cccatgcgct tttccgcacc ccagaaacaa cccatgccca gaacgacgta atccgtcgcc
     1801 tcgcgggacg ccgcggtttt ctccatcgca tatactccca taataacgct caggcctgct
     1861 gccatggcta taacagcctt catttgcaca ctcacctcca tccgggtctg atcttgtaat
     1921 actcgtagta cgagcactta cagcttcacg aaattctgtt gctaactgtt aaaacaggaa
     1981 tgccagcgca tcgccgatct cggtgcatct gtcgatcttg tcaaaaatat ctttatgttg
     2041 ctcgttctac ccacatccac gaactgcggc acatgtctcg gtgaatttga aacctgaaat
     2101 gaaaataaat tccgttgggt tacaaacgaa aagatgattc aggcctcccg tcctttggtt
     2161 ggcctgtttt cgcctgccta ttgatgggct tttaggggga ttaaacgacc tatcaccagg
     2221 ctagatctag tgatagatcg gtttgctcgc tcacaccctg ccttcattaa agctcgagca
     2281 gcaaaagtga gcagggatgc cattttttgg cgtattccgg ggggaactgc agttcggact
     2341 caaggcccgt aatatataaa taaaatgtta tggtaacaat caaaacgagt gacttttatt
     2401 tatagtaata ctgagtacaa ttagcaaact tcttcatgca ttgcgcatca gagttccacc
     2461 tagtataaaa ccagagtttc cctaatgttc caatgtcata gaccgcatta ggatggagcg
     2521 actttcactt catccatttc attggagttt acctatcatg gaaaaagaaa taaggaagtt
     2581 tgtagcggct atatctacgc tgggcctact agcagcctgc tcggggaccg atgcaatcga
     2641 agcaaggaag gacgacacaa gtgtgtccgc acgaaatatc aggccttttg tcgaccatga
     2701 cgagcttgcc aggcggtatg aaaaccgggc cgggaaactg ctggaaatag cagcggaaca
     2761 cgagaaacgg ctccagtatt acgaggacaa aagctacctc tacgggagac accagcagga
     2821 ttcacaagcg cataccatcg ccctagtgca aaaatacaag cgggcggcag agaaagccac
     2881 cgaatctgct tttcaccaca ggatggtgtc gcaacttgcg aaacgcgatt atattgcttc
     2941 gggggcgtca cattgatttg gattgccccg ggagctcagc ggtgaacctg gctggaactg
     3001 gatgaaacag tacgacttga ttccgtggca ctggccgcag attagctgtt cgcatctcat
     3061 tggggatttc ttttgaatgc cgctacatat tgaaacacca ttaattgaat ccccttcgat
     3121 tagtcataaa gtggggcaat ccatttggct gaaaatggag tccgcccaac catccggctc
     3181 tttcaaggcc cgaggaatcg gctatgcatg cgaagaatat ttaaaacgag gggcaaaacg
     3241 cttcattgca tcatcgggcg gtaatgcggg gctggcagtt gcttttgctg gacgcagcct
     3301 ttcagtgcct gtagtcatcg tggtgccgaa gacagcttca aagcgggcag tgaacctgat
     3361 gggtttagag aacgcggaag ttatagtcca tggggagtct ttggacgaag cacaccgatt
     3421 tgcgctgtca ctcatccaac ctgccgatgc ctatatacat cctttcgatg acccgctgct
     3481 ttggaaaggt catgccagtc taattgatga aatattccgt gcgggtctaa agcctgacgc
     3541 tatcgttctg tcagtcggcg gcggcggcct cctgtgtggt gttgtagaag gattgaagca
     3601 gaatgggatg ggcgatgtgc cgatcatcgc tgttgaaact gaaggcgccg actcctacgg
     3661 gaaatcgtta aacgcgggat tccttgtcga gcttccaaac atatcgagta tcgcgacgac
     3721 gttaggagcg cgcatagttt gtgaacaagc attggcgtac tctaaaactc accatatcaa
     3781 aagcgttgtg ttgccggaca agatagccgt ttctgcttcc ttgcagtttt tgcaagacca
     3841 tggaattgt
//
LOCUS       DASSHJ010000062        24546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_396307, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000062 DASSHJ010000000
VERSION     DASSHJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 24546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24546
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396307"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..1472)
                     /locus_tag="VFI43_02440"
     CDS             complement(450..1472)
                     /locus_tag="VFI43_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET7061017.1"
                     /translation="MQKSIGIWLLACCALVFAMVVVGGVTRLTDSGLSIVEWQPIVGT
                     VPPLSEKDWDELFDKYHQTPQYKKVNLGMSLDEFKGIFWWEYFHRLLGRVIGLAFFIP
                     FVYFLAKKAIDRSLGLKLTGIFVLGGLQGAMGWYMVKSGLVDNPHVSQYRLTAHLGLA
                     FLIYAAMFHVALELLSPARSSFQNIRLQGLHRFSTTITGLIFVMVLSGGFVAGIRAGL
                     AYNTFPLMNGHFVPPDIFVLEPWYRNFFDNMATVQFDHRMIAWALAILIPIFWYKSRG
                     VPLSGSARLACTLLLIMLAIQITLGISTLLLAVPLPLAAAHQAGALLLFTAALWVNHQ
                     LCGSQL"
     gene            complement(1550..1993)
                     /gene="smpB"
                     /locus_tag="VFI43_02445"
     CDS             complement(1550..1993)
                     /gene="smpB"
                     /locus_tag="VFI43_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7061018.1"
                     /translation="MSIVQNKKAFHDYFIEEKYETGVVFQGWEVKAIRAGRVQLKEAY
                     VIIRDSELFLIGCHISPLLAASTHINPDPVRTRKLLLHAEEIKRLIGKAERAGYTLVP
                     LDMHYKKGKIKLEIGLAKGKKQHDKRESEKQKEWDRDRQRLLRAR"
     gene            2084..2521
                     /locus_tag="VFI43_02450"
     CDS             2084..2521
                     /locus_tag="VFI43_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HET7061019.1"
                     /translation="MADIEKSVLIGYSASQMFALVDAVEDYPKFLPWCGGSSVTRQSD
                     KVTHATILIDYHHIKHSFTTENVRTPPELITMTLLNGPFKNLDGQWRFIPLSENACKI
                     EFRLHYTFSHIFLEKLVGPVFFMIANSFVEAFIERAEDIYGES"
     gene            2508..2843
                     /locus_tag="VFI43_02455"
     CDS             2508..2843
                     /locus_tag="VFI43_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HET7061020.1"
                     /translation="MESRDETIAIEVVYALPGHQVIRELRLPSGTTVKGAVERSGIAD
                     LYPEIDFARNKLGIFGKLVDPGRRLCAGDRVEIYRPLARDPKEIRRERARLRHNTSVA
                     KTFDRPDTA"
     gene            2925..4385
                     /gene="guaB"
                     /locus_tag="VFI43_02460"
     CDS             2925..4385
                     /gene="guaB"
                     /locus_tag="VFI43_02460"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7061021.1"
                     /translation="MRLVEKALTFDDVLLLPAHSAVLPRDADLRTRLTRSITLNVPLV
                     SAAMDTVTEARLAIALAQEGGIGIVHKNMPVEAQASQVANVKRFESGVVKEPITIPPD
                     MTVREVFNLIRRYKISGLPVVEGSKVVGIVTNRDLRFETNLDQPIKNIMTLKDRLVTV
                     NEGTTREEAMALLHKHRLERVLVVNADFELRGLITVKDIIKTSEHPNACKDEQGRLRV
                     GAAIGVGEGTDERAEALAEAGVDVIVVDTAHGHSQSVLDRVAWVKKRFPQIQVIGGNI
                     GTAAAAKALVEQGADAVKVGIGPGSICTTRMVAGVGVPQVTAIKNVADALAETGIPLI
                     ADGGIRYSGDIAKAIAAGAHSVMLGGLFAGTEESPGEIELYQGRSYKTYRGMGSLSAM
                     QQGSSDRYFQQTEGDSKKLVPEGVEGRVPFKGSVVAVIHQLIGGLRSGMGYVGCSTID
                     EMRAKAEFIEITSAGMRESHVHNVQITKEAPNYHVE"
     gene            4598..6160
                     /gene="guaA"
                     /locus_tag="VFI43_02465"
     CDS             4598..6160
                     /gene="guaA"
                     /locus_tag="VFI43_02465"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7061022.1"
                     /translation="MHQKILILDFGSQYTQLIARRVRESSVYCEIHPWDSAPHFIQAF
                     APTGIVLSGGPASVPVAETPRAPQVVFELGVPVLGICYGMQTMAAQLGGTVENGKIRE
                     FGYAEVEIIAPNVLLRDLNDRTGADGHEMLEVWMSHGDTVIALPSGFKVILQNAATPI
                     AGMADEERKFYGIQFHPEVTHTLQGKAIIERFVHEICGAGYDWNMPDYVAEAIGRIQS
                     EVGLDEVILGLSGGVDSAVAAALIHGAIGKQLTCVFVDTGLLRLREAEQVMETLNRNL
                     GVKVIHVDASKAFLGELHGVSDPETKRRVIGREFVEVFQREAAKIPNARWLAQGTIYP
                     DVIESAGGKMKKAQTIKSHHNVGGLPETLHLKLLEPLRELFKDEVRELGLTLGLPHDM
                     IFRHPFPGPGLGVRILGEVTEEYANLLREADAIFIEELRISGWYEKVSQAFAVFLPVK
                     SVGVMGDGRTYEYVVALRAVQTEDFMTAHWAELPYALLGKISNRIVNEVRGVNRVVYD
                     ISGKPPATIEWE"
     gene            complement(6352..6984)
                     /locus_tag="VFI43_02470"
     CDS             complement(6352..6984)
                     /locus_tag="VFI43_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7061023.1"
                     /translation="MLVDDHAMFRHGMEMLINREPDMAVAAEACDGEEALALLTPDHH
                     FDIVLMDVSLKTHSGLEVIKRVHNILPELPMLVVSMHDEAVYAERALRAGARGYVMKQ
                     EPGKVLVGAIRELLNGNLYLNKHIQARLLKRITAGNSKSEPLVSSLSPTEFEVLHLIG
                     AGHSTQEIARLLNRSIKTIETHRYNIRTKLNLKDAPELVRYAIQWVSEGQ"
     gene            complement(7017..9161)
                     /locus_tag="VFI43_02475"
     CDS             complement(7017..9161)
                     /locus_tag="VFI43_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7061024.1"
                     /translation="MIRKNVPSAIAPTTRAEESPGSAKELRERLKEITCLYESRHHMG
                     PELPLEDICRQIFDHLIPAMHFPAVASATVELDGRRFASENYREGSTHALQSNIAVRD
                     RLCGHLRVFYPDNTSFLLPQEQELVDAIAGDLEMWLKEINCLYEIRRSAELELPLEDV
                     CQEIFRHLIPALQFPKSATVVIELDDRRFVGEDDGHEGTHELRSKISANNKLCEQGRA
                     HELHADITVNNKLCGHLRVFYPLDKPFLLPEEQELIDAIAHDLERWLKEVNCLHEIRR
                     AASLELQVKDICQQIFEHLMPALQFPEAATVVIELDDNRVATGSHEKGLTHELHAKIS
                     ADNKLRGQLRVFYPLDKPFLIPEEQRLIDAVGVELGKWLEAKRIDETLLERLKEITCL
                     YEIRRNLGQELSFDNVCQHIFAHLIPAMQFPKIATAIIEVGSRRFISENHAQNLTHEL
                     VSAITVNKRLCGHLRVFYPENKPFLLPEEQRLIDAVARDLERWLEGKQLEQTVVSVAE
                     KYQRTIGQELHDNLGQQIAAIGYQAETLEQDMSASGITEAAKVAASIATQARSAVARC
                     KELAQGLLPFELETYGLIIALQALANRVGDAYKISCEFVRKNDIAIDDNKVALNLYRI
                     AQEAISNAIRHGGARHIDILLGSDSGVLYLSICDDGTGISGIDTEQGSSTGVGIKIMH
                     YRARQLSAELKFLSSPRGGTEVRLEMPLGREP"
     gene            9762..10169
                     /locus_tag="VFI43_02480"
     CDS             9762..10169
                     /locus_tag="VFI43_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061025.1"
                     /translation="MKTEIRKFIVAVSALGQLTSCVQTSAFELKNDDTSIPAQNSRTF
                     TDHDSIASRYENIAKELLVKAEEHKKLLEHYEDKSYLYGKRGQDFQSHKIALVRKYTL
                     AAEKAATQAAFHYRMASELTKRDYAAFREPPHH"
     gene            complement(10206..10427)
                     /locus_tag="VFI43_02485"
     CDS             complement(10206..10427)
                     /locus_tag="VFI43_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061026.1"
                     /translation="MIQINQVMGSLRKASAPHYTGLNTRRAIAPRLTSIFMLCRHIVI
                     VRVFRGFDITVPGIVRSLTPNAVPSKSGF"
     gene            complement(10897..11316)
                     /locus_tag="VFI43_02490"
     CDS             complement(10897..11316)
                     /locus_tag="VFI43_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7061027.1"
                     /translation="MQKIREVMSSDVHTISPDSSIEEAAQEMRDGDFGLLPVANNGQL
                     LGVITDRDITVRAVAEGKSPDTQIKEVMSGGVISANEDDTVEDAARIMSEHQIRRLPI
                     VDGKHHLVGIVSLGDFAVDASDIEPVMEALEGISSPP"
     gene            complement(11363..11512)
                     /locus_tag="VFI43_02495"
     CDS             complement(11363..11512)
                     /locus_tag="VFI43_02495"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET7061028.1"
                     /translation="MLYTIAVVLIILWLLGLVSSYTLGGFIHVLLVIAIVMILLRIIS
                     GRKPL"
     gene            complement(11775..11999)
                     /locus_tag="VFI43_02500"
     CDS             complement(11775..11999)
                     /locus_tag="VFI43_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7061029.1"
                     /translation="MNWDQIEGNWKQLKGKIKEQWGKLTDDNIDTIAGKKDQLSGRIQ
                     ESYGITKEEADKQISEWARQQKEDYPPRHH"
     gene            complement(12063..12647)
                     /locus_tag="VFI43_02505"
     CDS             complement(12063..12647)
                     /locus_tag="VFI43_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7061030.1"
                     /translation="MNIPNKLFVLTLSLAGALLISGCGKKEEGPKSGAPKTEAKTTVG
                     TEADDAAITTKVKSAFMGDADVKGSDIKVETRKGVVQLSGFVDNQAQMDRAVALAKGV
                     EGVKSVDNKMDLKTGSGTVGEKIDDAVITTKVKAALLGDSQVNSGDIGVVTQNGEVQL
                     SGFVNNQTQIDRATEVAKGVEGVKKVTNEMSVKK"
     gene            13590..15665
                     /locus_tag="VFI43_02510"
     CDS             13590..15665
                     /locus_tag="VFI43_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7061031.1"
                     /translation="MINIKSLTRYSNISLVLLLIVIGALFFHELTIREEITNGEHRRY
                     QALMLADELVQSSDDLTRMARSYVTTGDPKYKQHFYEISDVRAGKLPRPLNYSPAYWH
                     LVEAGRASAFQPGEAVPMLEMFRRAGFSEEEFSLLREALMNSNGLIEMETRAFAAVEG
                     LKDDGMGNFTVKAKPDPALALRLLFGPEYTDRKADIMAPIQEFMDLYNLRMENELNRK
                     LARLERQSLYEMFAVIVALIATMAIVFYTRLGILRPLAELGRQVATGTREMSAVRYGR
                     ASSNEVEKLGEALALADEEKRQLLENERAARNEAERASRLKDEFLATLSHELRTPLNA
                     ILGWSQLILSGNMKKEDVHRGLETIERNAYAQNRLIEDLLEMSSIISGKVRLDMHLLD
                     ADSLIEAAIESVRPTAQAKQVTLTRTIYPQSHLIMGDSSRLQQVFWNLLSNSIKFTPS
                     GGSIDVVVGQSALFLEIRITDSGMGISPEFMPYVFDRFRQADASLTRQHGGLGLGLAI
                     VKQLVELHGGSVRAESPGTGKGSSFIVQLPLVARNLDLKESVNAAGERNVRGARAPSF
                     KGLTILVVDDELDAREIIKRILTSRGAAVITAASAGEGLDIVRAKKPDLIISDIGMPG
                     KNGYEFLREVRVFQMKAGTYTPAVALTAFAHPDDACKALDAGYQRHLIKPIDSFRLIT
                     TIAELARQK"
     gene            complement(17130..17357)
                     /locus_tag="VFI43_02515"
     CDS             complement(17130..17357)
                     /locus_tag="VFI43_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061032.1"
                     /translation="MEIEWKVVDENHQEVFVEKHVRGLLWHTSAGFSFWHSYPNPGVD
                     VSQAGTLDEAKKTVEEALRMEEYQDPATLKN"
     gene            17691..17891
                     /locus_tag="VFI43_02520"
     CDS             17691..17891
                     /locus_tag="VFI43_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061033.1"
                     /translation="MRNYDYDDEYARYSGLLDQGAEVFTKKMFRHSQAKAEENWNDMK
                     KNRQTKKMFSDRSRLRRASYRT"
     gene            complement(18096..19472)
                     /locus_tag="VFI43_02525"
     CDS             complement(18096..19472)
                     /locus_tag="VFI43_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET7061034.1"
                     /translation="MIVGFGSIGQAVLPLILRHFEIAPERVTLVSADRGGEEIAREFG
                     VSFMLQALTPQNYESLLGSRLNKGDFVLNLSVDVSSLALIELCSRRGALYLDTCIEPW
                     SGGYTDTSIPASLRSNYALREQALSFGREKREGPTALLNHGANPGLASAFVKKALLDI
                     ASDTGCHTPKPVSPNGWAELAFRLNIRAIHVSERDTQIAEQRKKRDEFVNTWSVDGFV
                     GEGLQPSELGWGSHERHWPEEGERYGFGCDAAIYLNRPGASTRVRSWTPLAGSYHGFL
                     ITHGESISIADHLTLREGEGAIYRPTVHYAYHPCDDAVLSIHELAGRNWHVQRNKRIL
                     RDEITGGIDELGVLLMGHAKTAYWYGSRLSVDEARRLAPYNNATTLQVAAGILAGMAW
                     VLEHPREGVVEPDDIDYEASLEIARPYLGQLVGIYSDWTPLRDRDWLFKENLDTGDPW
                     QFKNVRVT"
     gene            19803..20156
                     /locus_tag="VFI43_02530"
     CDS             19803..20156
                     /locus_tag="VFI43_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061035.1"
                     /translation="MTNCNLPQHKDHTHPHGPECGHTGVIHEDHVGYLHEGHLHCAHD
                     DHFHEHAIAVSTANPQNCTPEHQCGGHSQGHVHGPECGHEAVPHGDHVDYLVEGHLHH
                     PHGGHCDNHGQVKIA"
     gene            20388..21071
                     /locus_tag="VFI43_02535"
     CDS             20388..21071
                     /locus_tag="VFI43_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7061036.1"
                     /translation="MIKAAETVVLVHGLWMHGLVMRLLGARLERCGFHTVCFSYPSMR
                     SSLSQNASELARFAAKIEAPRIHFIGHSLGGLLILQMLAEFPEIRPGRIILAGSPYNT
                     SCVAEKLSRHRAGRYILGRSMRQWLEQEPPPGAKDYEIGVIAGAKSIGVGRLISRLAT
                     PNDGVVTVGETRIPSTAEQIVLDVTHAQMLLSAELARQACSFLRWGRFTNDRKTAGYR
                     GLTERQAGR"
     gene            21068..22150
                     /locus_tag="VFI43_02540"
     CDS             21068..22150
                     /locus_tag="VFI43_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7061037.1"
                     /translation="MMRTGHFARLGLIWMCLWLGGCANLGYYAQAVDGHMDIHKRVRP
                     INEIIADPASDPQLKRTLSLVERLREFAKEELRLPDNQSFTTYADLGRRYAVWNVSAA
                     PELSVEPEKWCFVAAGCVSYRGFFSEAEANRFAEELKAKGYDTALGGIRSYSTLGWFK
                     DPVLNTFLGYSDIELAQLIFHELAHQLIYVQDDSTFNESFATAVELEGIDRWLKQQGD
                     ERQRAAFNAAQQRKTDSTALMLEYRKRLEAMYGSSLSPTEKRAAKASILAELREKIST
                     LEPGKSSVNYQKWLATHLNNAYLASVSTYTEWVSEFHTLLAKEEGDINQFYDRVRELS
                     ILPKAERIALLRKANERRLSASNTNP"
     gene            complement(22147..24060)
                     /locus_tag="VFI43_02545"
     CDS             complement(22147..24060)
                     /locus_tag="VFI43_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HET7061038.1"
                     /translation="MFLSSAAIGLAYNYSRTAIYRSSATLLTSAMTPIDRESNDADIQ
                     HVAIQRQILLGHELIVATLSRLKTSANDKSVLALTPADLQSLLTVDPVEDTNLVEIKA
                     EGPDPKLLPLLINTWIDVYLDARSDEVKRRTGTTTRILEDELKSLTAKINAARLDLES
                     FRKAYDISSTERDENEALARLKGLTDSLNKASEEEVKARANLNAIRTAISRGQAVVPE
                     DEKGSLLEIEKRLQDLREKLAELDKKFTREYLNLQPELRSIPEQIKELEAAVRTKRQH
                     GKNVMLTDAEMSYAAAQQTVREIRAQLDQHKKQASAFTSRFIQHDALKTDLEGLEKLY
                     RDTQERLVQVKASNREKYPQVSVISRAYEARDPIRPDYSRDALFVLAGSLLLGLFGVW
                     ISEYLTQKKEQQPPVAIFGIQKYAEPPATGLIDHSPGALSNPGHKAIETKTNHALAGP
                     VERELSSHQLRVLLDSANLKGKQLIGLLLSGLAIDEIISLTPDQIDLEAGTITVPGRA
                     PRTVPMGAMLKSFFRQSGGQPAWDPDDARSPIDLSAALVCAAVDSGLPEPDHITADAI
                     RHGYIAYLVRQGLRLSDLEQIVGYLDPDVLASYRAYSPPQQGRHLYDIELLHPALMSG
                     SGGLPAKGQTGHV"
BASE COUNT         5791 a         6559 c         6415 g         5781 t
ORIGIN      
        1 attgcaaggt cgggcgtgca gctttccacc ttccccaatg aggtcatcgt gggcgccagg
       61 atctcaaagt cgggatcccc aatgccgcag agtggtgatc ttcagggtct cagcgagccg
      121 atcagggtag gagcgtccgg aatcaacgtc gtgatcgacc agcaactgcc ttaacggcgc
      181 tcgcttgcgc tcccgctcga ttgagaatga tctgagcttc cctctcccgc tcgactgaga
      241 gtgatttgag cctccctctc ccgcttgggg gagagggaaa gagggagagg gtgttttaaa
      301 aagaaagcac gtccagccca tcccttcagt actgatcctt tcctcgatcc cacttcgcgc
      361 cccccttacc cttcactggc agtaggcgaa cgtaaacata ctgcggaatc accctcctgg
      421 tctttatcct gctaacgacc ctactcgagc tataactggg agccgcacaa ctggtgattg
      481 acccataagg ccgcggtaaa cagcaggagt gcaccggcct ggtgggctgc tgcgagcgga
      541 agcggaactg ccagcaataa ggtggaaatg cccagggtga tctgtattgc cagcatgatc
      601 agcagaagcg tgcaggcaag gcgtgccgac ccggaaagcg gcacccctct cgatttatac
      661 cagaaaatcg ggatgaggat ggccagggcc caggcgatca ttcggtggtc aaactgaaca
      721 gttgccatat tatcgaagaa gttacggtac catggctcca gcacgaatat gtcgggcgga
      781 acgaaatggc cgttcatcag cggaaaggta ttgtaggcga ggcctgcgcg aattcccgct
      841 acgaaaccgc cggataaaac catcacgaag atcagccctg tgatcgtggt ggaaaaccgg
      901 tgcaagccct gcaaccggat gttttgaaac gatgagcgtg ctggcgacag aagttccagc
      961 gctacgtgga acattgcggc ataaataaga aaagccagcc ctaaatgcgc agtcagtcgg
     1021 tattggctga catggggatt atcgaccagg ccgcttttta ccatgtacca gcccatcgct
     1081 ccctgcaacc cccccagcac gaaaatacca gtcagcttga ggccaaggga ccggtcgatc
     1141 gcctttttag cgaggaagta aacaaaggga ataaaaaagg caagcccgat gacccggccg
     1201 agcaagcggt gaaaatattc ccaccagaaa atacccttga actcgtccag gctcatgccc
     1261 aagtttactt ttttatattg cggtgtctgg tggtatttgt caaaaagctc gtcccaatcc
     1321 ttctcactga gggggggaac agttcccacg atgggctgcc attcaacgat tgaaaggccg
     1381 gaatcagtga gccgggtcac ccctccaaca acaaccatgg caaataccag ggcgcagcac
     1441 gccagcagcc agataccgat agatttctgc atttccggat gagaaattaa caatagacgt
     1501 tgaccatgct caccaagtgc cgaagccccg gatcgatgat cggctcagat cacctcgcgc
     1561 gcagaaggcg ttgcctgtcc cgatcccact ccttctgctt ctcggattca cgcttgtcat
     1621 gctgtttctt gcctttggca aggccgatct cgagcttgat tttgcccttc ttgtagtgca
     1681 tgtccagcgg caccaacgta tagccggcac gctcggcttt cccgatgagc cgctttattt
     1741 cctccgcatg caggagaagc tttcgagtcc ggacggggtc agggttgatg tgggtcgaag
     1801 cagccagcaa ggggctgata tggcaaccaa tgaggaataa ctcgctgtct cgaatgatga
     1861 cgtaggcttc ttttagctgt acgcgcccgg cgcggatcgc tttgacctcc catccctgga
     1921 aaacaacgcc tgtttcgtat ttctcctcga taaaatagtc gtggaaggct ttcttattct
     1981 ggacaatact catttacagg aggaaaaagg atgagttccg cttggttttg gtgtatttta
     2041 acagcttttg ccgcttgtct ggcttgatca ggcagcttct cctatggcgg acatcgagaa
     2101 atcagttttg atcggttatt cggctagcca gatgtttgca ttggtggatg cagttgagga
     2161 ttatcccaag ttcctgccgt ggtgcggcgg ctcttccgtg actcgccaga gtgacaaggt
     2221 gactcacgct acaattctga tcgattatca ccatatcaag cacagcttca cgactgaaaa
     2281 cgtacggaca cctccagagc tgatcacgat gacgctgctc aatggtccct tcaaaaatct
     2341 cgatgggcaa tggcgtttca tccctctttc cgaaaatgcc tgcaagattg aattccgcct
     2401 gcactacacc ttctcgcata ttttcctgga aaagcttgtc ggacccgttt tcttcatgat
     2461 cgcgaacagt tttgtcgaag catttatcga gcgggcggaa gatatctatg gagagtcgtg
     2521 acgaaacaat cgcgatcgaa gttgtatacg ctctgcctgg gcaccaggtg attcgcgaac
     2581 tgcgccttcc cagcggtaca acggtaaaag gagctgtgga gcgttcagga atcgccgatc
     2641 tataccccga gatcgacttt gcacggaaca agctgggaat ctttggaaag ctggtcgacc
     2701 cgggcagaag gctgtgcgcg ggagatcgcg tggaaatcta tcgtccgctg gctcgcgatc
     2761 cgaaagaaat tcgcagggag cgcgcaagac ttcgtcacaa tacaagtgtg gccaagactt
     2821 tcgacagacc tgatactgct tgaagtcccc ctccggcgtt gcatgaagcc caaccgcatg
     2881 cttcgcggtt tcgctataat gcgagtttgc aaaggatcaa taccatgcga ctggttgaga
     2941 aagccctgac ctttgacgat gtactgctgc ttcccgccca ttccgcagtt ttgccgcgcg
     3001 acgccgatct caggacaagg ctgacgcgct cgatcactct caatgttccc ctcgtttctg
     3061 ccgccatgga caccgtcact gaagcgcggc ttgccattgc gctcgcccag gaaggcggaa
     3121 tcggaattgt ccacaagaac atgccggtcg aagcgcaggc ttcccaagtg gcaaatgtca
     3181 agcgattcga aagcggcgta gtgaaggaac ccatcacgat cccacccgac atgaccgtgc
     3241 gggaagtttt caacctcata cggcgataca agatatcggg gctaccggtg gtcgagggat
     3301 cgaaggtcgt aggtatcgtc accaatcggg atcttcgctt tgaaaccaat ctcgatcaac
     3361 ccatcaagaa tatcatgacc ttgaaagacc gcttggtgac cgtgaacgaa ggcacgacgc
     3421 gggaagaggc catggcgctg ctgcataagc accggttgga aagggttctt gtggtcaacg
     3481 ccgattttga gctgcgcggg ctgattacag tcaaggatat catcaagacg tccgagcatc
     3541 ccaacgcatg caaggatgag cagggccgtc tgcgtgtagg agcggccatc ggagttggcg
     3601 agggcaccga tgaacgtgcg gaagccttgg ctgaagcagg cgtggatgtc attgtggtgg
     3661 atacagccca tggacactcg cagagcgtgc tggatcgcgt ggcctgggtt aagaagcgct
     3721 ttccgcagat ccaggtcatt ggcggcaata tcgggaccgc cgccgcggcc aaggctcttg
     3781 tggaacaggg agcggatgct gtgaaggtag gcattgggcc tggctcgatc tgtactacgc
     3841 gcatggtcgc aggcgttggc gtaccacaag taaccgcgat caagaatgtg gctgatgcct
     3901 tggctgaaac aggcattccc ttgatcgccg atggcggcat ccgctattcg ggtgatatcg
     3961 ccaaggcaat tgcggcgggc gcacattcgg tcatgctggg tgggctgttc gctggaaccg
     4021 aggaatcgcc tggggaaatc gaactttatc agggacgttc atataaaacc taccggggca
     4081 tgggatcgct ctccgccatg cagcagggtt cgagcgaccg ctattttcag cagaccgagg
     4141 gggattcaaa aaagctcgtg ccggaagggg tggaaggccg tgttcctttc aagggaagcg
     4201 tggttgcggt gattcaccag cttatcggag gattgcggtc cggcatgggg tatgtgggct
     4261 gcagcacaat tgatgaaatg cgcgcaaaag ccgagttcat tgaaataacg tctgccggaa
     4321 tgcgggaatc gcacgttcat aatgtgcaga tcaccaagga agcaccgaat tatcacgtcg
     4381 aatagtctcc cgctcgaggg ggccgacccc tgaaaaattc ccgataacgg gatgtagacc
     4441 tttttccgag ctgattcccg aaacccttcc cgcggccccc tctcctgcca cttatttttg
     4501 atttttccct ggcttcgacg aacgccaaag actttccagt attctttcta tccgtgttct
     4561 atttcaacta cccgtccgtt ctcctgtccg cttacccatg caccaaaaga tactcattct
     4621 tgacttcggc tcacaatata cccaactgat tgcacgacgc gtacgtgaaa gcagcgtgta
     4681 ttgcgagatt catccgtggg attcggcacc tcactttatc caagcgttcg cacccactgg
     4741 aatcgtcctt tcgggaggac ctgcctctgt ccccgttgcg gagacgccgc gagcacccca
     4801 ggtcgtgttc gaactggggg ttccggtgct gggcatctgc tatggaatgc agaccatggc
     4861 ggcgcaattg ggcggcacag tcgaaaatgg caaaatccgg gagttcggat atgcagaggt
     4921 agagataatt gccccaaatg ttctgctgcg cgatctgaat gacagaacag gtgcagatgg
     4981 gcatgaaatg cttgaggtat ggatgagcca tggcgataca gttattgctc tgccatccgg
     5041 attcaaagtt atccttcaaa atgccgctac gcctatagcg ggaatggcgg acgaggaacg
     5101 taaattctac ggaatccagt tccacccgga agtcactcat actctccagg gaaaagcaat
     5161 tatcgagcgc ttcgtccatg aaatctgcgg agccggttat gactggaata tgcccgatta
     5221 tgtggcggaa gcgattggcc gaatccagtc agaagtgggc ctggatgaag ttatcctcgg
     5281 cctgtctggg ggggtggatt cagcggtcgc cgctgccctg atccatggcg ctatcggtaa
     5341 gcaactgacg tgcgtttttg ttgacacggg cctgcttcgg ctcagagaag cggagcaggt
     5401 catggagacg ctcaaccgca acctgggcgt gaaagtcatc cacgtcgacg cgagcaaggc
     5461 ttttctaggc gaattgcatg gcgtgtccga ccccgagacc aagcgacgcg tcatcgggcg
     5521 cgaatttgtc gaggtatttc agcgcgaagc agcgaaaatt cctaatgccc ggtggctggc
     5581 ccaaggaaca atatatccag acgtcataga atcagcggga ggcaaaatga agaaagccca
     5641 gacgatcaaa tcgcaccata acgtaggcgg actccccgaa acgctgcact taaaactgct
     5701 tgaacccctg cgtgaactgt tcaaggacga ggtgcgagag cttggcctga cgctgggcct
     5761 tccgcatgac atgattttcc gccatccctt tcccggcccg ggcctcgggg tgcgcatcct
     5821 gggcgaggta acggaggaat acgcaaatct gttacgggag gctgacgcga tcttcatcga
     5881 agaattgcgc atttctggct ggtacgaaaa agtctcccaa gccttcgccg tcttcctgcc
     5941 ggttaaatca gtaggggtta tgggcgacgg acgcacctat gagtatgtgg tcgcgctacg
     6001 cgccgtgcag accgaagact tcatgactgc gcactgggcg gagctcccct atgcactcct
     6061 ggggaaaatt tccaaccgca tcgtgaacga agtgcgcggg gtcaatcgag ttgtttacga
     6121 tatctcgggc aagccgcctg ccaccataga gtgggaatga ttttttcacg caattaacgg
     6181 catcaggggc taggacgagc aggagaagag agatctgatg cctgccttgc acgccatact
     6241 tgacaattgc acggttagac ggcaactgcc ggatgaggtg gccgctcgct actaccacta
     6301 agcctattgg cttttatatc cggagccgtt ttgccacccg tgcaacgatt tctattgccc
     6361 ttccgaaacc cactggatgg catatcgtac caactctggc gcgtctttca aattcagttt
     6421 ggtacgtatg ttgtagcgat gggtttcaat agttttaatg ctgcggttga gcaagcgggc
     6481 aatttcctga gtgctgtggc ccgctccaat taaatgtaat acttcaaact ccgtagggct
     6541 aaggctgcta accaatggtt cagattttga atttccagcc gttatccgct tcaataatct
     6601 cgcctgtatg tgcttgttca ggtataaatt tccattcaat agttcgcgga tagcgcccac
     6661 gagcaccttg cccggctctt gcttcatcac gtaacctcgc gcgccggccc gcaaggcacg
     6721 ctctgcatat actgcttcgt catgcatcga cacaaccagc atgggcaatt cgggaagtat
     6781 gttatgcacc ctctttatga cctcaagtcc ggaatgggtt ttgagagata cgtccatcaa
     6841 aacaatatca aaatgatgat ccggcgtgag aagcgccagc gcctcctcac catcacacgc
     6901 ctccgcagcc actgccatgt cgggctcgcg attgataagc atctccatgc catgccggaa
     6961 catcgcgtga tcgtccacga gcatcacccg tgctttcgcg gccgaggctc ccactcttaa
     7021 ggctcccgac ccagcggcat ttctaatcgc acttccgtcc cacctctggg agaagacagg
     7081 aatttcagtt cagcactcag ctggcgggcg cggtaatgca tgattttaat tcctactccc
     7141 gtggaggagc cctgttccgt gtcaataccg gaaatgccgg tgccatcgtc gcatatggac
     7201 aggtagagca ctccgctgtc ggagcctagc aaaatgtcaa tatgacgcgc accgccatgg
     7261 cgtatcgcat tgctgatcgc ttcctgcgca atccggtaaa ggttaagggc aaccttgtta
     7321 tcatcgatgg cgatgtcatt cttccggacg aattcgcagc taatcttata ggcgtcccca
     7381 accctgtttg cgagtgcttg cagtgcgatt atcaagccat aggtttccaa ttcaaacggg
     7441 agcaagccct gcgcgagttc cttgcatcta gcgactgcgc tccgcgcttg cgttgcgatc
     7501 gacgcggcaa ctttcgcggc ttctgttatg ccggaagccg acatgtcctg ctccagggtc
     7561 tccgcttgat aaccaatcgc tgcaatctgc tgcccaagat tgtcgtgcaa ttcctgcccg
     7621 atcgtacgct ggtatttttc cgcaacggaa actactgtct gctccagttg cttgccctcg
     7681 agccatctct ccagatcacg cgcgaccgcg tcaatgagtc tctgctcctc aggtaacagg
     7741 aagggcttgt tttccgggta gaaaaccctc aaatgcccgc acagcctctt gttcactgta
     7801 atggccgaca ccagttcgtg cgtaagattc tgagcatgat tctcagaaat gaaacgcctg
     7861 ctgccgactt cgatgatggc agtagcaatt ttggggaact gcattgccgg gatcagatgc
     7921 gcaaaaatat gctggcatac attatcgaac gataattcct gccccaggtt gcgtcgaatc
     7981 tcgtagaggc aagtgatttc cttcaggcgc tcgagcaagg tttcatcgat tctttttgcc
     8041 tcgagccatt tccccaactc aaccccgacg gcgtcgatca gtctttgttc ctccggtatg
     8101 agaaagggct tgtccaaagg atagaatacg cgtagctgcc cacgtaattt gttatccgcg
     8161 ctaatctttg catgcaactc atgcgtaaga cccttttcgt gacttccagt agcaacccga
     8221 ttatcgtcga gttcgatcac gacggtcgct gcttctggga attgcagcgc cggcatcaag
     8281 tgctcaaaga tttgctggca aatatccttc acctgcaatt cgaggcttgc ggcgcgtcga
     8341 atctcgtgca ggcagttcac ttccttaagc catctctcca ggtcgtgcgc gattgcatca
     8401 attaactcct gctcttcagg cagcaggaat ggcttatcta gagggtagaa cacacgcaga
     8461 tgtccgcaca gcttattgtt tactgtaatg tccgcgtgca gctcgtgcgc ccttccctgc
     8521 tcacacaatt tattgttcgc gctaattttc gagcgcagct catgtgtacc ctcgtgccca
     8581 tcatcctcgc ccacaaaccg tcggtcatcg agttcgatga cgacagtggc ggattttggg
     8641 aattgcaatg cgggtatcag gtgccggaag atttcctggc agacgtcctc cagcggcaat
     8701 tcaagctccg cgctgcgtcg aatctcgtac aggcaattaa tttccttaag ccacatctcc
     8761 agatcgcccg cgatcgcatc aacgagttcc tgctcctgcg gtagtaaaaa cgacgtattg
     8821 tcaggataaa acacgcgcaa gtgcccgcac agcctgtcgc gcactgcgat gttcgattgc
     8881 agcgcgtgcg tactaccctc acgatagttc tccgaggcaa aacgccgccc atcgagttcg
     8941 accgtggcgc tcgcgactgc cgggaagtgc attgccggta tcaaatggtc aaaaatctgc
     9001 cggcaaatat cttccagggg taattccggc cccatgtggt ggcggctctc gtaaagacat
     9061 gtaatctcct tcaaccgctc acggagctcc tttgcgctgc caggagactc ttcggcccgc
     9121 gtagtgggag ctatagcact cggtacattt ttgcgaatca tgccggctct cctccttccc
     9181 tcaatctgat gtcttttccc gttccatgga ggttgtcgac tcttaattga tcatgctttc
     9241 ggaaatagct tactgaaact tttttacaac gctgcccggc tggtaacgcg ccgtctgacg
     9301 acctattttc agctgtcaat gaaagactag ttatttctga atggaaagtt gtaataaaac
     9361 ggttaaattt tggttaaatt ttcatggaag agaggaaagc tatggaagaa gcggcggaag
     9421 gccggcatca agctgcttgc gcaaattgaa cctctacaaa gaggcaaggt gaggatggga
     9481 ggctttgggg caggccgtcg aggcagccat gactgatgcg tgcgcaagct attgcccatc
     9541 gtaaacgtgg tcatgaaaac tcaaaccccc gggaagaaat taccgaccgc ttgacgcagt
     9601 tgctctctcg tcgcttcttt ctgcgttgct aagggtttca cctagtcttg ctcaaggttt
     9661 cgcttagcaa aaaacgagag tttcccccaa tgttcccatg gtcttagctg tattaggatg
     9721 aaaccgcttt acttcattca cttcattgga gggcatccat catgaaaaca gaaataagaa
     9781 agtttatagt ggctgtttcc gcactcggcc aactgacttc gtgcgttcag accagtgctt
     9841 tcgaactaaa gaacgacgat acgagcatac ccgcgcaaaa tagcagaact tttaccgatc
     9901 acgatagtat tgccagccgg tatgaaaata ttgcgaagga acttctggtg aaggcagagg
     9961 agcacaagaa actgctcgag cattacgagg acaaaagcta cctctacggg aagcgggggc
    10021 aggatttcca atcgcataaa attgctttag tgcgaaaata cacgctggca gcggagaaag
    10081 ccgctacgca ggctgctttc cactacagga tggcgtcgga gcttacgaag cgcgattacg
    10141 ccgcttttcg ggagccgccg catcattaag caacaggtaa aacaaggcca agatccgatc
    10201 ggattctaaa aacccgactt ggaaggaaca gcattgggag taagggagcg gacgattcca
    10261 ggaaccgtga tatcgaagcc tcgaaaaacc cgaacgatga caatatggcg gcatagcatg
    10321 aagatgctgg tcaatcttgg ggcgatagcg cggcgggtgt tgaggccagt gtagtgagga
    10381 gcactggcct ttctcaagct gcccatcacc tgatttattt ggatcactgg tctctaaaac
    10441 ccattgaaag cttgaagtaa tgaagaggag acatagtaat tcctggccgc gccagacggg
    10501 gaaatgctga ttgaaatggg gctaacacgt ggagaagaag caggatgagg gcaaggtgtg
    10561 gtgcaagcaa acaccgatgc aacactcaga cggaaaagag gaatggaacg ataaaatcac
    10621 actaccgaac aattgaatcg gatctgacaa ctgcaccagt caaatcgggg taactgccag
    10681 atgaaatcgc gactgctaca gataagatca cacactaaaa attgaatccc atctcatctc
    10741 tcaaatcggg caactgccaa accgagtaat ttcggcgagc ttactactac acgtatgtcc
    10801 acaactggaa catccgttac ttgaggaaga cagcaggaga tcgcactccg ctatacataa
    10861 gacgcatagc gcgatcttcc tgacgtttta ccgcgttcac ggtggactcg agattccttc
    10921 aagcgcttcc atcactggtt caatatcgga agcgtcgacc gcgaagtcgc cgagtgacac
    10981 gattccgacc agatggtgct tcccgtccac gataggaagc cgccggattt gatgttcgct
    11041 catgatacgg gctgcatctt ccacagtgtc atcctcgttg gccgatataa ctcccccgct
    11101 catgacttcc tttatctgtg tatccgggct tttgccctcg gcaactgccc tcaccgtgat
    11161 atcacgatcg gtgatgacgc cgagcaactg accattgttc gccacgggca gcagtccgaa
    11221 atccccatcc cgcatttcct gcgcagcttc ctcaatggaa gaatcgggac tgatagtatg
    11281 tacatccgaa ctcatcactt ccctgatttt ttgcatgtcc gtctccttgt ctgaaagtcg
    11341 ggcggcggcg cttgatatac ttctatagcg gctttcgccc actgatgatt ctgaggagga
    11401 tcattacaat tgcgattacc agaagaacgt gtataaagcc gcccagtgtg taggaactta
    11461 ccaatccgag cagccataag atgatcaaga caacagcgat tgtatagagc atgatgttcc
    11521 tctcttttat gaattatcca tgactctcca gatggttgcc ttgctgccgt tgttaaccag
    11581 ccggggagtg catccgccac tttttctgct tggtcgtctc tccttgttct ctgcaccgtc
    11641 agacttgagc tgctgccgcg gctttctgct tttattatta ttgtttgaag gtttaaagct
    11701 gaactcgtcc tgaactgcct tcttccctca tttgatctcc cccatgagac aaggggagat
    11761 caacgggatt atgcttagtg gtgccgtggg ggataatcct ccttctgctg cctcgcccac
    11821 tcggagatct gcttgtcggc ctcttccttt gtaataccat aggattcctg gatccttccg
    11881 gatagctgat cctttttacc ggcaatagta tcgatattat catcagtgag cttgccccat
    11941 tgttccttga tcttgccctt caattgtttc cagtttcctt ctatttggtc ccagttcatt
    12001 cttttactcc ttcgtgttca agttaataaa gctgctgaac gcttccttcg ttatcggcat
    12061 ggctatttct tgacgctcat ctcattggtc accttcttga cgccctcgac gccttttgcg
    12121 acctcagtag ccctgtctat ctgggtctga ttgttcacga atccgctaag ctggacttct
    12181 ccgttttggg tcacgactcc gatatcgcca ctatttactt gagaatcacc cagcaacgct
    12241 gcctttacct ttgttgtgat cacggcatcg tctatctttt cgcccacggt cccgctaccg
    12301 gttttcaaat ccatcttgtt atccacgctc ttcacgcctt caacaccctt ggccaatgcc
    12361 actgcgcgat ccatttgagc ctggttgtca acgaagccgc tcaattgcac gacacccttg
    12421 cgcgtctcca ccttgatgtc gcttcctttc acatccgcgt cgcccatgaa cgcggacttc
    12481 accttcgtgg taatggcagc atcatcggcc tcggttccta cggtagtctt ggcttccgtt
    12541 tttggggccc cggattttgg gccttcctct ttttttccac agcctgaaat caacagtgca
    12601 ccggcgagcg acaaggtcag gacaaataac ttatttggga tattcatgct tgttttctcc
    12661 aaattaaagt aaaaattaac gaaatggttg gctgccaatg ctgtcgcaga gaaaccgacc
    12721 tctgcccccc tttggagcgc atttggcagc tgaatggttc ggggtggaaa gcgctcagca
    12781 gaacggctat aacttggcca actctcgcgt tacccttgag cctttagcag ctccgagtta
    12841 accaatatca gtcaaataaa tcccgctgcc attggccttt ctacggtttc catcatgcga
    12901 cttgcaggcc aacggctcag tgcgctgtcg cacataaatg caaatcgccg tttgttttga
    12961 acggaaaaaa tgggtcgacg gaggtggaag ttaatagata aggtgaggat tcatcattat
    13021 ttttctcctt tacggaaaaa aaaagcaaac atgctttcag catgtcactc attcaggaaa
    13081 tgacagggga aggaacgaac atgaatcgcc aaggatcctg gaggcagatt gagcagccaa
    13141 aggtttcaag gagaaggcct catggttcca acagcctgca gcggtaatag gggacattct
    13201 tgttttgcaa tttccgtctg atcaagcagg tgttagcgtg agaagtctca tttttgtcgg
    13261 ccctttctac gaaataagga ttgaacaatc cacagctgtg atttccggga tacaagtatt
    13321 gaagacggct cccacaggtc gtgcacatag ccagacctcc aactgctaga ttgattacgt
    13381 acgaccgcat tttgttttgt cttgcctgtc ggccagcgaa ttgcccggat ctgacgaagg
    13441 cttgggctgc gaactttaaa cactccggca ccccagcagt actggcgaaa acaaggtaat
    13501 cgggaagtgt gaaaacagga gtagacacaa tctgaaaccg gacatataga attccatctg
    13561 ctcacccatt agatcgcacg gaatacctcg tgataaatat caaaagcctc acccgatatt
    13621 caaatatctc tcttgtcctg ctgctgatcg ttatcggtgc gctcttcttc catgaattga
    13681 caatccgaga ggaaatcact aatggcgagc atcgccgcta ccaggcattg atgttggcgg
    13741 atgaattggt gcaaagttcg gatgacctga cccgaatggc gcgctcttat gtcaccactg
    13801 gagatcccaa atataagcaa catttttatg aaatatctga cgttcgagcc gggaaattac
    13861 cgcggccctt gaactatagc cctgcttact ggcacttggt ggaagcagga cgagcatcag
    13921 ccttccaacc gggtgaagct gttcccatgc tggagatgtt ccgtcgagca ggtttcagcg
    13981 aggaggaatt ttccctgctc agggaagcgc tcatgaattc aaatgggttg atcgaaatgg
    14041 aaacacgggc ctttgccgct gtggaagggc ttaaagatga cggcatgggc aattttacgg
    14101 taaaggcgaa acccgacccg gcacttgctc tccgcctcct gttcggtccc gaatacactg
    14161 accgtaaagc tgacatcatg gcgcctattc aggaattcat ggatttgtat aatctgcgca
    14221 tggaaaacga actgaaccgg aagttggccc gccttgaacg tcaaagcctt tatgaaatgt
    14281 ttgccgttat cgtcgcgctg atcgccacca tggcgattgt cttttatacc cggcttggaa
    14341 ttttgcggcc tcttgcggag cttggccggc aggtagcgac gggaacccgg gagatgtccg
    14401 ctgttcgtta tggaagggca tccagcaatg aggtagagaa gcttggcgaa gcactggcgc
    14461 tggctgatga agagaaaagg cagctgctgg aaaatgagcg cgcggcaagg aacgaagcgg
    14521 agcgcgcgag ccggctcaag gacgagttcc tggccactct ttcccatgag cttaggaccc
    14581 cgctcaacgc catcctcgga tggtcccagc tcatcctgag cgggaatatg aaaaaggaag
    14641 atgttcatcg cgggcttgag acgatcgaac gaaacgccta cgcgcaaaac cggcttattg
    14701 aagatcttct tgaaatgagc agcatcattt cgggaaaagt caggctggat atgcatctgc
    14761 tggacgcgga cagcctgatc gaggcagcca ttgaatccgt cagaccaaca gcgcaagcga
    14821 agcaggttac cttgaccaga acgatctatc cacaatccca cctgatcatg ggcgattcca
    14881 gccggcttca acaggtcttc tggaatcttt tatcgaactc catcaaattt acgccgagcg
    14941 gtgggagtat cgatgtcgtt gtggggcaat ccgcactttt tcttgaaatc aggataaccg
    15001 attcaggcat gggaatttcg ccggaattca tgccctacgt cttcgatcgc ttcaggcaag
    15061 ccgatgcttc gcttacgcgt cagcacggcg ggctcggtct tggactggca atcgtcaagc
    15121 aactggtgga acttcatggg gggtctgtcc gggcggaaag tccgggaacc ggaaaaggtt
    15181 cgtcatttat cgttcagctc ccactggtgg caaggaacct tgatctgaag gagagtgtga
    15241 acgccgcggg ggagcgaaat gtccgcggcg cgagagctcc ctccttcaaa ggcctgacaa
    15301 tactggtcgt tgacgatgaa ctggatgcac gggagatcat caaacgtatt ctgacgagcc
    15361 gcggggccgc ggtaatcacg gctgccagcg cgggagaagg actcgacata gtcagggcca
    15421 aaaagccgga cttgatcatc agcgacatcg gaatgccggg taaaaacggt tacgagttcc
    15481 ttcgggaggt acgggttttt caaatgaagg caggcaccta cacacctgcc gtagcgctga
    15541 cagcatttgc gcaccctgac gatgcctgca aggcgctgga cgccggctac cagagacatt
    15601 tgatcaagcc catcgattca ttcaggctga tcacgaccat tgcggagctt gcccggcaga
    15661 agtgaaagtg cgccggaggt caccagcgat aacctcaccg agcgcaaaag ctcaagtgtc
    15721 ctgcctccgg atccccactg ctagattcct acccgttcat ccactgttga aagcgtcaaa
    15781 tcatatgagc atccaacgag gcgtctggtc gaagggatat agaggcccac cgcgtctgtg
    15841 ctgcctcgaa catagttaca cgttttataa atccctcaac aacttgaggg atattcctga
    15901 gatattggat gaattccttg agaagccagc ccaatctcga tggaaaagtt ttcttgacat
    15961 ttgaaaataa tccacatcgc caataaaagg aatgtcatta gcgtccagct ccgcgaaaag
    16021 gcgcatggat gcttggtctt atatatatgt atctaattct aaagaagttt ttaaaagtac
    16081 aaaaattaac cacaagtgaa aatactgttt cgaaaggccg acttaggagt agtgttcatg
    16141 ccttatcaac aaacttatcc acagcaactg tggaaaataa aagctctgca aagcagtcga
    16201 caaggataaa tcgttccgtg cttttaccgt cgtcagaacg gacaatgtaa ttcgacgcgg
    16261 aactgacgcg gaactgagtt aggcgcagaa acaagagccc ttggtacata cgctctaatt
    16321 tttagtggag tgttcaccgg ggtttcgaag gccgaacggt cgatgagaaa gcaattggag
    16381 aactggcagt aacaaaatca ttctggccca aagaaaaaga tgagcccgac cgcaagccat
    16441 gagagaaacg catgggtggc taagtttaga acgtgcttcc cctcgtcaca ccgcctcagc
    16501 agtggagcaa attatcaagg accggttacc ctctccctca gaggcgtaaa ccctgacttc
    16561 ggctcaccgc tcggataatt ccttacaaaa ctgttgtgcc gcaagaaatg gcataaagag
    16621 agcattcttc tcgctgattg aagcatgctc cacgtcacac tgtaaaggat tccgaaaaag
    16681 ccaagcataa tcacatctat tgatggctaa aaaagcaaaa attccgcttg acaatttcac
    16741 agtaatccac atctgcccct tagccccggc aacgcgtcca aagcccatcc atgcagacac
    16801 gcatgaacat tagcgcttca tttatttatt tgatttataa gtattttttt ctcagcgcaa
    16861 aaattagcca aaaaagaaaa tatccttacg aaaaggcgac ttatacgccg tgtcaatagc
    16921 ttatcaacaa acttatccac agcaattgtg gacaacggca gttcgccttg tgatgcgatt
    16981 tcagaacata gaggagagca gataactcga aaatcaccaa gtccgctagt tgcggccaac
    17041 ttctatcttc cccgattacc cgggaagctc tctttttccc cggagaccag cagaccgaaa
    17101 gcggtcctca caggcatccc ttagtcatgt caattcttta gtgtcgcagg gtcctggtat
    17161 tcctccatac gaagggcttc ttcgaccgtt ttttttgctt catccaaggt gccggcctgg
    17221 gaaacgtcca cgcccggatt tggatagctg tgccaaaagg aaaaacccgc gctggtatgc
    17281 cacagcaagc ctcttacatg cttttccacg aacacttcct gatgattttc atcaacgact
    17341 ttccattcaa tttccatcgc tcgctccctg atattatgcg gtcaaagccc gcggttgctt
    17401 ctacacaccg gatctcaagt atacccgtcc gatatctttt ccccaaacat cagccacata
    17461 ccaagcgcta ccaatactac ggttccgttg ccgcctcccg cttttacaga tgacagcgaa
    17521 aagcctgctt gcgtctctgc acgacagaac gtaccgcgcg ttactaagtt cggtatcgca
    17581 cagtatttcg ttctggatgg attatcttat cgtgcaagca cgaagtacga cattaacgat
    17641 ggaaacctgt tcgttttaca tcaatttctc gattgaagac ggaggcggaa gtgagaaact
    17701 acgattacga tgatgaatac gctcggtaca gcggcctgct tgaccaaggg gccgaggttt
    17761 tcacaaaaaa aatgttcagg cactctcaag cgaaagcaga ggaaaactgg aacgatatga
    17821 aaaaaaaccg ccagacaaaa aaaatgtttt ccgaccggtc gcggttacgc cgggcaagct
    17881 accgtaccta gctctcctgg cccgcaagca gggccactgt cggttacgcg agacgcttgc
    17941 ggctgttccc ctcggctggg gtgagtcctt agccttgagg atgagtatct cgtccttggc
    18001 tgccatcctg aatggaacgg actctggccg ggaacagtca tagagcgttt ctatgacagg
    18061 actcgtgacg gtagcgagta ttgtggccgt ttccttcagg ttacgcggac gttcttgaac
    18121 tgccatgggt cgccggtatc gagattctcc ttgaatagcc agtcacgatc gcgcaggggt
    18181 gtccagtcgc tgtatattcc taccaattgc ccgagatacg gccgggcaat ctcaagagaa
    18241 gcttcatagt ctatatcgtc aggctcgacg acgccctcgc gaggatgctc caacacccaa
    18301 gccatccccg ccagaatccc ggcagccacc tgcagagttg tcgcattgtt atacggagcc
    18361 aaccgccgtg cttcgtctac agaaaggcgc gacccgtacc agtaggcggt ttttgcatgc
    18421 cccatcagca agactcctag ctcgtcgatt cctccagtga tttcatcccg caggatcctc
    18481 ttgttccgct gcacgtgcca attcctcccc gccagttcgt ggattgagag cactgcgtcg
    18541 tcgcaggggt ggtaagcgta gtgaacggtg ggccgataaa tcgctccttc accctccctc
    18601 aaggtcaaat gatcggcgat ggaaatggat tcgccatgcg tgataagaaa gccgtgatag
    18661 gagcccgcaa gcggtgtcca gctgcgcacg cgcgtggatg cgccaggacg attgaggtaa
    18721 atcgccgcat cacagccaaa cccgtagcgc tcgccttcct cgggccagtg acgttcgtgg
    18781 ctgccccatc ccagctcgga aggctgaaga ccctcgccca cgaacccgtc caccgaccat
    18841 gtgttcacga attcgtcccg tttcttgcgt tgctcggcga tctgcgtgtc acgctcggag
    18901 acgtgaatcg ccctgatatt caagcggaaa gccagttcgg cccagccatt ggggctcacc
    18961 ggtttcggcg tatggcagcc ggtatcagag gcaatatcaa gcagcgcctt tttaacaaac
    19021 gccgaggcca accctggatt tgcaccgtga ttcagcaacg ccgtaggtcc ttcgcgcttt
    19081 tcgcggccga acgagagggc ctgctcccgc aaggcgtagt tagaacgcaa agaggcagga
    19141 atcgaggtat ccgtgtaccc ccccgaccac ggctctatgc aggtatcgag atacagcgct
    19201 ccgcgccgcg agcataattc gatgagggcg agactggaaa catcgacgga gagattgagc
    19261 acaaaatcac ccttattcaa gcgcgagcca agcaagctct cataattttg tggtgtcaac
    19321 gcctgaagca tgaagcttac gccgaattcg cgcgcgatct cctcgcctcc tctgtcggcc
    19381 gagacgagcg tgacacgctc cggtgctatt tcaaagtggc gcaagatcaa aggaagcaca
    19441 gcctgcccga tactgccgaa accgactatc atcaggctac ccggcaaata tccatgtttt
    19501 atatgtcctt caattcccat tgttgttttc ctggttttta aaacctgttt ttcttttcta
    19561 ttggggttgc cttgtctcac gcaaggcaga agtttgcccg ccctcctcac gcgaggaccg
    19621 aacgaccgga cgaccagtag atatgaacag gaactgtgcg ttcccaaagg ggtcgaaccg
    19681 ttctaatata caaagaacag ttaagcagtc tgttatttat ccgcttgcct gaacatgcct
    19741 gctcttcgct gtgcttgcgc ggccgcgggc tgacaggctc gattcacttg gagggaacga
    19801 taatgaccaa ttgtaacctg ccacagcaca aagatcatac tcatccgcac ggccccgaat
    19861 gcggccatac tggggtcatc cacgaggatc atgtcggtta tctgcatgag ggccatctgc
    19921 attgcgcgca tgacgaccat tttcatgagc acgcgatagc ggtctcaacc gccaatccac
    19981 aaaactgcac tcccgagcat caatgcggcg ggcattcgca ggggcacgtg catggccccg
    20041 agtgtggaca tgaagccgtg ccccacggcg atcatgtcga ttaccttgtc gaaggacacc
    20101 tccaccatcc gcatggcggg cattgcgaca atcacggtca ggtaaagatt gcctgacaag
    20161 aggattgcgt gcttgcgatt gccaagccag tatctcccag cgcagcctgc tcacaggtgg
    20221 gaacgaaaga gcttcaagcc tgagtcctgc tggcgtttct aatttagcct tcgcattatg
    20281 gattcgggcg gcatttatct tccacttgag acattcggga gaatcaagca gagagccggc
    20341 tgagaattga actttcgagc ggacgaagaa gcagtggaat cattgttgtg ataaaggcgg
    20401 ctgaaacagt cgtgctcgtg catgggctct ggatgcatgg cttggtgatg agactgctag
    20461 gcgcgcggtt ggaacgttgc ggcttccaca cggtctgctt ttcctacccc tccatgcgtt
    20521 cctccttatc ccaaaacgcg tcggagttgg cacggtttgc cgccaaaatc gaagcgccac
    20581 gcatccattt tatcggccac agcctcgggg gtctcctgat actgcaaatg cttgccgagt
    20641 ttcctgaaat ccggcccggc cggataattc tggccggaag tccctataac acaagttgcg
    20701 tcgctgaaaa actctcccgt cacagagccg ggcgctatat tttggggcgc agcatgcgcc
    20761 agtggcttga gcaggagcct ccccccggtg caaaagacta cgaaatcggg gtaatcgcgg
    20821 gagcaaagag cataggcgta ggcaggctga tctcccggtt ggcgacaccg aacgatggag
    20881 tggtcactgt cggtgaaacc agaatcccca gtactgccga acagattgtg ctcgatgtca
    20941 cccacgccca gatgctacta tccgctgaac ttgcccggca agcgtgttct ttcttgcggt
    21001 gggggcgttt cacaaatgac cggaaaaccg cgggataccg cggattgaca gaaaggcaag
    21061 caggcagatg atgagaactg gacactttgc gcggctcggc ttgatatgga tgtgcctttg
    21121 gctgggtgga tgcgccaatc tcgggtatta tgcccaggca gtggatggcc acatggacat
    21181 tcacaagcgg gtgcggccga taaacgaaat tatcgccgat cccgcttccg atccgcagtt
    21241 gaagcgaacg ctctccctgg tagagaggtt gcgtgaattt gcaaaggagg agctcaggct
    21301 cccggacaac cagagcttta ccacttacgc tgatctagga cgccgttatg ccgtctggaa
    21361 tgtgtcggct gcgccggagc tatcggtaga gcctgaaaaa tggtgctttg tggcagccgg
    21421 ttgtgtcagt taccgcggct ttttttcaga agctgaagca aaccgttttg cggaggaact
    21481 taaggcaaag ggttatgaca cggcgttggg ggggatacgc agttattcga cgttggggtg
    21541 gttcaaggac ccggtattga atacttttct cggttattcg gatatcgagc ttgcccagct
    21601 tatattccat gagctcgcgc atcagcttat ctacgtgcag gatgacagca cgttcaacga
    21661 atcgtttgcc acggcggtcg aactcgaagg gatagaccgc tggctgaagc agcagggcga
    21721 cgaaagacag cgcgccgcat ttaatgctgc gcagcaaaga aaaaccgact caacggcgtt
    21781 gatgctggag tatcgcaagc ggctggaagc catgtatggg tcgagtttga gcccgacgga
    21841 aaagcgggcg gcaaaagcaa gtattcttgc cgagctgcgt gagaagatat ccacgctcga
    21901 acccgggaaa tcctccgtca attatcaaaa atggctggcg acacatctca ataatgccta
    21961 cctggcttcc gtctccactt acaccgaatg ggtatcggaa tttcacacgc tgctcgcaaa
    22021 ggaagaggga gacatcaacc agttctacga tagagtaagg gagcttagca tattgcccaa
    22081 agccgagcgg atcgcgctgc tgcgcaaagc gaatgagcga cgcctttcgg cgagcaatac
    22141 gaacccttag acgtgtccgg tctgtccctt tgctggcagc ccgccactcc cactcatcaa
    22201 ggccggatga agcagctcga tgtcgtaaag gtgccgtcct tgctggggcg gcgagtaagc
    22261 acggtatgag gccaggacgt ccggatcgag gtaaccgacg atttgctcga gatccgataa
    22321 tcgcaagcct tggcgcacga ggtaagcgat gtagccatgc cggatcgcgt cggcggtgat
    22381 gtggtccggt tctggcaatc ccgaatccac tgccgcgcag accagggccg cggaaaggtc
    22441 aatgggcgac cgggcgtcat cgggatccca tgccggctgc cccccggatt gcctaaaaaa
    22501 tgatttgagc atggccccca taggaacagt tctgggcgct ctaccgggca cagtgatggt
    22561 tcccgcttca aggtcgatct ggtcgggcgt caaggatatg atttcatcga tggcgagccc
    22621 gctgagcaag agtccaataa gctgctttcc tttcaggttg gcagagtcaa gcagaaccct
    22681 gagctggtgg cttgagagtt cccgctccac cggaccggcg agtgcatgat tggttttcgt
    22741 ctcgatcgcc ttatgtcccg ggttactcaa ggcaccggga gaatgatcga tcaacccggt
    22801 agctggaggt tcggcatatt tctgtattcc gaaaattgct accggcggct gctgttcctt
    22861 tttctgtgta aggtactcgg atatccatac gccgaaaagc cctaatagca gcgaacccgc
    22921 gagtacgaac aaggcatcgc ggctataatc gggccggatt ggatcccgtg cctcgtatgc
    22981 gcggctaatg acactgacct gcggatattt ctcgcgattg ctggccttca cctgcaccaa
    23041 ccgctcctgc gtgtcgcgat agagcttttc cagaccttca agatcggttt tcagcgcgtc
    23101 atgctgaata aacctggacg tgaaagccga ggcctgtttc ttgtgctggt ccagttgagc
    23161 cctgatttca cggactgtct gctgggctgc cgcataactc atttcggcat ccgttagcat
    23221 cacgttcttg ccgtgctggc gcttcgtgcg gactgcggct tcgagctcct tgatctgttc
    23281 ggggatcgat cgcaattccg gctgcaggtt gaggtattcg cgcgtaaatt tcttgtccag
    23341 ttccgcgagt ttctcccgca gatcttgcag ccgcttctca atctcgagca gactgccttt
    23401 ctcatcttcc gggacgacgg cctgccctct cgaaatcgct gttctgatcg cattcaggtt
    23461 agccctcgcc ttcacttctt cttcgctcgc cttgttaagt gaatcggtca ggcctttgag
    23521 gcgcgccagc gcctcgttct cgtcccgctc ggtcgatgaa atatcatagg cttttctgaa
    23581 agactccaga tccagtcgag ctgcgttgat ttttgcggtc aagctcttca gctcatcctc
    23641 gagaattcga gtcgtcgtgc ctgtccgcct tttcacctca tcggagcggg cgtcgaggta
    23701 aacatcgatc caggtattga tgagcaaagg caggagtttg gggtcgggac cttccgcctt
    23761 gatctcgacg aggttggtgt cttcgactgg atcgacggtg agcaggctct gcagatcggc
    23821 gggcgtcagg gccagcacgg acttgtcgtt cgccgatgtc ttgaggcgtg acaacgtggc
    23881 gacaatcaac tcgtgtccca gcaaaatctg acgctggatc gccacgtgct ggatgtcagc
    23941 gtcattgctc tcccggtcga tgggggtcat cgccgacgtg agcagggtgg cgctgctgcg
    24001 ataaatggcc gtgcggctgt aattgtatgc cagccctatc gctgcactgc tcagaaacac
    24061 gatgccaaaa atgattaatc ttcgagaccg gtaccacggc ttctcactct gagtggcgga
    24121 cggatacatt cttcctgtga tgcgctgatt gatcatttct ataattgacc gttaaccgga
    24181 gtgcctcgaa agtatctcgt tcgtatacct gatgcaaaca atcgttctgt acggtttcgc
    24241 acaaacaact gctttgcgtc tgctcacaat acgatcataa gtccaaagaa aagcgttttt
    24301 tcacattcct gtaaaggttc tgatttaagg tgagggtggc aacagtattg taaaacaacc
    24361 ggatttgacg gggatatcgc tattcttagg gctctgacca agtccgccat ggggtaattc
    24421 gatggagagg gagaacttgt tcagagagat tcgcgtttcg gtcgcgtcat acgcatgttt
    24481 cgtcgattgc gttatggttt gttcagcatt ttggttttca cccctttaac aggaatagat
    24541 gatgaa
//
LOCUS       DASSHJ010000063         5394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_612531, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000063 DASSHJ010000000
VERSION     DASSHJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612531"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3031)
                     /locus_tag="VFI43_02550"
     CDS             complement(<1..3031)
                     /locus_tag="VFI43_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7061039.1"
                     /translation="MTLTILEKGARMSRSIGLKSRGALGYPSRQVKYTPRQIAIHIVL
                     LAVGYFAGGKLGLALPYLNGEISANITLFWPPTGIALATLLTWGFSCWPGIYLGSIAI
                     NLTTGDLSVPAALAIGIGNTLAPVCAVFLLEQAARFQASFVKGRDVVSFMLFASGAML
                     ISATGGTLSLLAGGKLEPATALQAWLGWWLGDILGVLIFAPLLLLWATRRLDSLDNII
                     RSPQLRMEFGAVVFVCILTTWLVFGNSWSIGPLQLSLGFLVFLPLIWAGLRFDALGAS
                     ISTLIISLIAVWATARGLGPFARGGVWLDHVILCTFITTVSLVSLVIIVIQAARRQSE
                     HTTKDSESRLRLALKAANQGLFDLDVRSGVMKVSPEYARMLGYDAETFMETAPTWLDR
                     VHPDDREQFHKIYNDYIAGLRGNYQAEFRQYTRQGNWKWILSLGNVVEWDSHGFPSRM
                     LGTHTDISRQKQVEEELLKAMEDFRASEKLQREQRSLAEREQSRMRALLSAMNIGILF
                     EDNEHRIEYVNPAFLKMWGIGEHVDLVGKPTRAVLEQSVEYAARPPLTSKDILADEPG
                     NRLELDLSNGRTLTQTSHGVSDAEGHSLGRLWIYEDITYQRQAAQQLLYMAERDPLTG
                     LYNRHRFQEQLENMIASSIRNKTQFALLYFDLDDFKYINDTFGHRAGDLVLVRAAGEI
                     SSIVRHIEMFARVGGDEFAILSGIQPGEDISSLPARIVAAIASIPLQLGDTNVHLTSS
                     VGVAIFPTHGETAEDLVAHADAAMYQAKNLGKNTWTMYDSARDSSRAMVHHMSWHNKI
                     TQALEKNLYELHFQGVFHAATSTLSHLEVLIRMRDLNDPSNLIMPGQFIPVAEKSKQI
                     LHIDRWVIRRSIELLGHYPNLPPLAINISGRTFDEPEIPQFIHNLLNEWNVSPNRLII
                     ELTETAAVSDIQDAQRFIEAIGQTGCRVCLDDFGSGFSTFGYLKYLNAQILKIDGLFI
                     CNLPDNRDNQIFVKAMVEVARGLGKITVAECVEDAATL"
     gene            complement(3211..3285)
                     /locus_tag="VFI43_02555"
     tRNA            complement(3211..3285)
                     /locus_tag="VFI43_02555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3251..3253),aa:Val,seq:cac)
     gene            3503..4042
                     /locus_tag="VFI43_02560"
     CDS             3503..4042
                     /locus_tag="VFI43_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7061040.1"
                     /translation="MKSLIILAIALMAPNLASAIDKANLSDAQVAAIVVAANQIDIDM
                     GQLAESKSPNPEVKAFAHRMITDHTDVNKQATDLVTKLKVTPEENTISRALKSDAERN
                     RERLRKLSGKDFDKLYVADEITFHKQVLDTLDNKLLPSAKNDELKALLVKVRPAIVSH
                     LEHAEKLLTIVHEMNYKEK"
     gene            4320..>5394
                     /gene="gltX"
                     /locus_tag="VFI43_02565"
     CDS             4320..>5394
                     /gene="gltX"
                     /locus_tag="VFI43_02565"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7061041.1"
                     /translation="MVRTRFAPSPTGYLHIGGARTALFSWAFAKRHGGKFILRIEDTD
                     LERSTAQSTQAILDGMAWLGLDYDEGPFYQMQRLERYHEVAEALLNSGDAYYCYCTRE
                     ELDAMREQQRAAGLKPRYDGRWRDSTATPPAGVKPVVRLKNPVDGVVAFNDLVKGEIT
                     VANSELDDLVLLRADGVPTYNFGVVIDDLDMGITHVIRGDDHVNNTPRQINILRALNA
                     PLPQYAHVPMILGADGERLSKRHGAVSVMQYHEDGYLPEALINYLARLGWSHGDEEIF
                     SREQLVEWFDLASINRSPAKFNPEKLKWLNQQYLKNTDDDRLARLLTPYLEADGCDPA
                     SGPDLQKIAALLKERASTVAELAD"
BASE COUNT         1264 a         1428 c         1514 g         1188 t
ORIGIN      
        1 ccagggttgc cgcatcctcg acgcattctg caaccgtgat tttgcctaac ccgcgcgcga
       61 cctctaccat ggctttgacg aagatctgat tgtcacggtt gtcgggcagg ttgcatatga
      121 acaagccgtc tattttcaat atctgtgcgt tcaaatactt gagatagcca aacgtggaaa
      181 aaccgctgcc gaagtcatcg aggcaaaccc ggcatcccgt ctggccgata gcttcgataa
      241 aacgttgcgc atcctggata tctgacaccg cagctgtttc ggtcagttca atgataaggc
      301 ggttcgggct tacattccat tcattcaaca gattatggat gaactggggt atctctggtt
      361 cgtcaaatgt gcgcccggat atattaatgg ccagtggagg taaatttgga taatgcccca
      421 gcagctcaat gcttcgcctg atgacccacc gatcgatatg caagatctgc ttgcttttct
      481 cagccaccgg gataaattgc ccaggcatta tgaggttgga agggtcgttg aggtctcgca
      541 tccgaatgag tacttccagg tgactgagcg ttgaggtggc cgcatggaag actccctgga
      601 aatgcagctc gtatagattc ttctcaagcg cctgagtaat tttgttatgc caggacatgt
      661 ggtgaaccat ggcgcgcgaa gaatcccgag ccgaatcgta cattgtccac gtgttcttgc
      721 cgagattttt ggcctgatac attgccgcgt cggcatgggc aaccaggtct tcggcagtct
      781 cgccatgcgt tggaaagatg gcgaccccga cgctgctcgt gaggtgcaca ttggtatccc
      841 cgagctgcaa agggatggag gctatggctg ctacaatgcg ggcgggcagt gagctgatat
      901 cttctccagg ctggattccg ctcaaaatcg cgaattcatc accgcccacc cgcgcgaaca
      961 tttcgatgtg acgaacaatg ctggagattt ctccagccgc gcgcaccagt accagatcgc
     1021 cagcgcgatg cccaaaggtg tcattaatgt acttgaaatc gtcgagatcg aagtagagca
     1081 acgcaaactg cgttttattg cgtatcgagg acgcaatcat gttttcaagc tgctcctgga
     1141 agcggtgcct gttgtacaag ccagtgagcg gatcccgctc tgccatataa agcagttgct
     1201 gggcggcctg gcgctgatac gtgatgtctt cgtaaatcca gagccgtccg aggctatggc
     1261 cttccgcatc ggatacccca tgtgacgtct gggtaagcgt tcgtccattg ctcaaatcga
     1321 gttccagcct gttccccggt tcgtctgcca ggatatcttt cgacgtcagg ggcggcctcg
     1381 cggcgtactc gaccgattgc tccagcactg ctcgcgtcgg ctttcccacg agatccacgt
     1441 gttccccaat cccccacatt tttaaaaaag ccgggttgac atactctatg cggtgctcat
     1501 tgtcttcaaa caggataccg atgttcatgg ctgagagcag ggcgcgcatc cggctttgct
     1561 cgcgttcagc cagcgatcgc tgttcgcgtt gcagtttttc ggacgcgcgg aaatcctcca
     1621 tcgcctttag cagctcttcc tcgacttgtt tctgcctgct gatatcagta tgggtaccaa
     1681 gcattcggct cgggaatccg tggctatccc attcaaccac gtttcccagc gaaagaatcc
     1741 atttccagtt gccctgccgc gtgtactgcc ggaattctgc ctggtaattc ccgcgcagcc
     1801 cggcaatata gtcattgtag attttatgaa actgttcgcg gtcatcgggg tggacacgat
     1861 caagccatgt tggagctgtc tccataaaag tctcggcatc atatcccagc attctggcat
     1921 attccgggct cactttcata acgccggaac ggacatcgag atcgaacagt ccctggttgg
     1981 cggccttgag cgcgagccga aggcgcgact cgctgtcctt ggtagtatgc tcggactgcc
     2041 tgcgcgcagc ctggatgaca atgatgacaa gggacacgag cgaaacggtg gttatgaacg
     2101 tgcatagaat gacgtgatcg agccagacac caccccgtgc aaaggggccg agtcctcgcg
     2161 ccgtggccca gacggctatg agcgagataa tcagcgttga gatggaggct ccgagcgcat
     2221 cgaagcgaag acccgcccag atcaggggca gaaacaccag gaagcccagg gaaagctgta
     2281 gcgggccgat gctccagcta ttgccaaaaa ccagccatgt cgtcaggata cagacgaaaa
     2341 cgaccgctcc gaattccatc ctcagttgcg gcgagcggat gatgttgtcg agcgagtcca
     2401 gccgccgggt tgcccacaga agcagcaggg gagcgaaaat gagtacgccg agaatatcgc
     2461 cgagccacca gcctaaccac gcttgaagcg cggtggccgg ctccagcttt cctccggcca
     2521 gcagggagag ggtgccgcct gtcgctgata tcagcattgc gccggaggcg aacagcatga
     2581 aggacacgac gtcccggccc ttcacgaagc ttgcctggaa gcgtgcggct tgttcaagca
     2641 gaaatacggc gcagacagga gcaagtgtgt tacctatgcc aatggcgagg gcagcaggaa
     2701 ctgaaagatc tccggttgtc aggtttatcg caatcgatcc gagataaatt ccgggccaac
     2761 aggagaaccc ccaggtcaga agcgtcgcca gtgcaatgcc cgttggcggc cagaacaacg
     2821 ttatatttgc actgatctcg ccattgaggt atggcaaagc aaggccgagc tttcctccgg
     2881 caaaataccc gactgccagc agtacgatat gaatcgcaat ctgtcttggg gtatatttta
     2941 cttgcctcga gggatagccc aaggctcctc ttgatttcaa gccgatagaa cggctcatgc
     3001 gagcaccctt ttcgaggatt gtcagagtca tgggtgggag cagcaggaga aactgctcca
     3061 ggagaagcta ctctttcatg gcttcgggtt gcgcacagcg gagcgcggga ttaccttaca
     3121 gtagcgacaa tgtacagccc ttggtccggg gaaaaaggga cgaggagagg cagctttata
     3181 ccccctttga gggcctttga gaacgtctga tggtgggcgg tactgggatc gaaccagtga
     3241 cttccaccgt gtgaaggtgg cactctaccg ctgagttaac cgcccggtct gccagccccg
     3301 ggatgttgaa agggctgagg agaggccgca attagagcat gaacaagcgc ttccggtcaa
     3361 cgcgaatggc agatggctca tggttgaaga actgctagct gatctgtatg ttatcgtaca
     3421 gaaaatctcc ttgaaaagcg cgaggatgag ttacacctct tgaaggagag gtgccgccga
     3481 tttttaatct aaaggagccc caatgaaaag tctgattatt ctggccatcg ctctgatggc
     3541 ccccaacttg gcgagcgcaa tagataaagc caatctcagt gacgcgcagg tggcggctat
     3601 cgtggttgcc gcaaaccaga tcgatattga catgggtcaa cttgccgaat cgaaatctcc
     3661 taatcccgaa gtcaaggcgt ttgcccaccg aatgatcact gaccatacgg atgtcaacaa
     3721 acaggcaacg gatctggtaa ccaagctcaa ggtgacaccc gaggaaaata cgatcagtag
     3781 agccctcaaa tcggatgccg aaagaaaccg tgaaaggctg agaaaattaa gtggcaagga
     3841 tttcgacaag ctttatgtcg ctgacgaaat tacttttcat aagcaggtgc ttgatacact
     3901 tgataacaag ctgctgccca gtgccaagaa tgacgagttg aaagccctgc tggttaaagt
     3961 aagaccggca atcgtttcac acttggaaca tgcggagaag ctcctcacca ttgttcacga
     4021 aatgaattac aaggaaaaat aaaactctcc cgccgtggtc gaaatgcgaa gtatccgcct
     4081 gatttcaatc cagtgagatc ggcttatggc cgatctcctg gcgaccggcg tttaattttt
     4141 acctggattg tttagagtcg ggaaggcaaa taggctgggc gacgttcgcg cttaatatct
     4201 tgtctgggtt tcacgcttcc tcccgggaag tgcgttatga agaacgggag cgcggagctg
     4261 gcgtagaata gtgacctgtc tcttcgtctc atctggtttc aataatcttg ttcctcacca
     4321 tggtacgcac ccgtttcgcc cccagtccca ccggttatct ccatataggc ggggcgcgca
     4381 ccgccctgtt ttcctgggct tttgccaagc ggcatggagg caagttcatc cttcgaattg
     4441 aagataccga tctcgagcgc tcgaccgcgc agtcaacgca ggcgatccta gacgggatgg
     4501 cctggctggg tctggattat gacgaagggc cgttttacca gatgcagcga ctggagcggt
     4561 atcacgaggt ggcggaggca ttactgaact ccggggacgc atattactgt tactgcacaa
     4621 gggaagagct cgacgcgatg cgcgagcagc agcgtgctgc cgggctgaag ccgcgttacg
     4681 atggccgctg gcgcgattcc acagcaaccc cgccggcagg agtgaagcca gtggtgcgtc
     4741 tgaagaatcc ggtggatggc gtggtggcct tcaacgatct tgtgaaaggc gagatcaccg
     4801 ttgccaacag cgagctggat gatcttgtgc tgctgcgcgc cgacggcgtg cctacctaca
     4861 actttggcgt agtgatcgac gatcttgata tgggcatcac gcacgtgatc cggggcgacg
     4921 accatgtgaa caatacaccg cggcagatca acatcctcag ggcgttgaat gcgccgctgc
     4981 cgcagtatgc gcatgtcccg atgatccttg gcgccgatgg ggagcgtctt tcaaaacgcc
     5041 acggcgcagt ctcggtgatg cagtatcacg aagatggtta tctccccgag gcgctgataa
     5101 attaccttgc gcggctcggc tggtcacatg gtgatgaaga gatattcagc cgggagcaac
     5161 tggtggaatg gttcgatctt gcatcgatca accgctcgcc agcaaaattc aatccggaga
     5221 aactgaaatg gctgaaccag caatatctca agaatactga cgacgatcgc ctcgcacggc
     5281 ttcttacccc ttatctggag gcagacgggt gtgacccggc atcgggaccg gatctgcaaa
     5341 agattgcagc acttctcaag gaacgagcga gcacggtagc ggaactggcg gacg
//
LOCUS       DASSHJ010000064         6452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1375146,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000064 DASSHJ010000000
VERSION     DASSHJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375146"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1254
                     /locus_tag="VFI43_02570"
     CDS             115..1254
                     /locus_tag="VFI43_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7061042.1"
                     /translation="MKLELLGEISPKKFLRDCWQKKPLLIRQALPGFTGLLSREQLVE
                     LACRDDAQSRLIVQAKGKWKVKHGPFQQRALSRLPETHWTLLVQDVNHFFRSARDLLL
                     RFNFIPYSRLDDLMVSYAPEGGGVGPHFDSYDVFLLQGMGRRQWQISAQQDSELVANA
                     PLKILRNFKPEQEWLLEPGDMLYLPPGYAHNGIAKGECMTYSIGFRAPSYQELATQFL
                     VYLQDHCNLPGMYQDPDLKPRSHPGKISSAMLRQAGAALDKIKWTSEDVERFMGIYLT
                     EPKPHVFFKRPSQPVNEQEFTLRAEQEGLELDLKSRMLWKGSNFFLNGEFCRAGNAAG
                     TVLKALADHLEIQPAEPLNEQVLPILYQWYLDGFVTFSERVKKLQ"
     gene            1742..1975
                     /locus_tag="VFI43_02575"
     CDS             1742..1975
                     /locus_tag="VFI43_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061043.1"
                     /translation="MKYSLLAIALATLTLGACDQMKEHKPGEGKPGQYPPSLWEKREG
                     QVSDAEIEAAQKADAEAAAKQQQQSGSSTEPPK"
     gene            2101..2373
                     /locus_tag="VFI43_02580"
     CDS             2101..2373
                     /locus_tag="VFI43_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061044.1"
                     /translation="MKYFPLLAISLATLTLSACEYKKHPMDKPPPSAMAERDSAPKFD
                     ELDKEESKGGAGSGEAMTTAPGPSAAGARTEPPGTGAPGAMTEAPK"
     gene            2535..2837
                     /locus_tag="VFI43_02585"
     CDS             2535..2837
                     /locus_tag="VFI43_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061045.1"
                     /translation="MKYSLLAALLVALSLTACGRPKQALPENSTTYGTRTEEGTTLDQ
                     ERGAAATAPAAPGDKTAAAPGAQEDEYSGQAVLPGHALDGNGNPIKEGQSAIPAGH"
     gene            3083..3289
                     /locus_tag="VFI43_02590"
     CDS             3083..3289
                     /locus_tag="VFI43_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061046.1"
                     /translation="MRLTLVAAALTVLTLTACGKPSQALPEQEKANFSKITGQASPTS
                     GAPATPPASGNAAGGSSDKTEGSK"
     gene            complement(3408..4175)
                     /locus_tag="VFI43_02595"
     CDS             complement(3408..4175)
                     /locus_tag="VFI43_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7061047.1"
                     /translation="MRFACLGSGSRGNGLVVEAGKTRVLLDCGFTLKETIMRVSRLGI
                     EPDVISGIVVTHEHDDHIAGVARFARRFGIPIWLTYGTLRSAERSFAGWPGIKIIEDY
                     QRFAIGDIEVQPYPVPHDAQEPAQFVFSNGATRLGVLTDTGISTPHIEATLSRCHALV
                     LECNHDAELLANSDYPWRLKQRIASRFGHLENAASANLLASLDCSRLQHIIAAHLSQK
                     NNTPLLARTVLSSALNCTLDWIGVAEQDDGFGWRQLI"
     gene            complement(4244..>6452)
                     /locus_tag="VFI43_02600"
     CDS             complement(4244..>6452)
                     /locus_tag="VFI43_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7061048.1"
                     /translation="SILIVDDRETDVAVMKSMLRAGGYVSVSSTTNPLEVCDLHRAHR
                     YDLILLDLMMPRMDGFQVMEALREIENIGPGETLPVLVTTAQSENKKRALQAGAKGFI
                     AKPFDQFDVLTHIRDVLEIRLLQKELHRSAIGLRGEQSERPVARAASSRETEELFDQL
                     AVSLPNIFWIWDVKDEMFRYVNPSWENMTGRRVTSGDRLEKLFDAIHPEDKQRVMHES
                     RDLSDISMDHDYRLLLADNSVRWVHSRTFPVKDSKGVHRVVGIMDDISHRKEAHQPVW
                     PLPHYDSLTSLPNRTLFYESLRKTIKQAEAGHRIVSVLYLDIDNFKNINDTLGRAAGD
                     EVLRQFSLRLLECLRVTDTVARVGGDEFGCILVTPDGSGDAGMVASKIREALRLPFNI
                     EGTSVILTASMGISVYPSDSMDADTLVKNGDTAMYRSKKAGRDTYRFFTAEMNARAVQ
                     KLDQENALRKALELNQFVLHYQPKVELTQGRITGVEALLRWDRPGYGFTQPDEFISIL
                     EQTGLISQVGAWVIETACKQIAEWRNMGIGEIAVSVNVSGRQFSQATLSQDVVRFIEE
                     NGVHPQLLEFELQTERALRQNSIDSDLLELELTEGSVAMHATKMIGILNRLKKLGIRI
                     SVDDFGTGYSSLAYVKRFPIDVLKIDRSFITDVTTNAADAAITGAIIDMAHSLNVRVV
                     AEGVETIEQLDFLRQRGCDEIQGYYFSEPLSAAEITNLLKSKTLLRPAQFLVS"
BASE COUNT         1590 a         1756 c         1586 g         1520 t
ORIGIN      
        1 ggctgatctt gataaaatgt tcatgcgcgg aaaataggat atggccagct tgctccaatc
       61 tgccgcccac tgcctctcac ccctcaccca gggaacaaac gtaataccta tcacatgaaa
      121 ctcgaactgc tgggcgagat ctcgccaaaa aaatttctcc gcgactgttg gcaaaaaaaa
      181 cccctgctga tccgtcaagc tctgccgggc tttacgggtt tactgagccg cgagcagctt
      241 gttgagcttg cctgccggga tgatgcgcaa tcgaggctga ttgtccaggc gaaggggaaa
      301 tggaaggtca aacatggccc gttccagcag cgcgcccttt caagacttcc ggagacgcat
      361 tggactttgc tggtacagga cgtcaaccat ttctttcggt cagctcgcga tttactgctc
      421 agattcaatt tcatcccgta ctctcggctt gacgatctga tggtcagcta cgcacccgag
      481 ggtggtggag ttggcccaca tttcgattcg tacgacgtct ttttgttgca ggggatgggg
      541 cgaaggcaat ggcagatctc ggcccagcag gatagtgaac tcgtcgcaaa cgcgccgctg
      601 aaaattctca ggaatttcaa gcccgagcag gaatggctgc tggagccggg cgatatgctg
      661 tatctgccgc cgggctatgc gcacaacggg atagcaaaag gcgagtgcat gacctattcg
      721 atagggtttc gggcaccgtc gtaccaggaa ttggcgacgc agtttctcgt ttacctgcag
      781 gaccattgta acctccctgg aatgtaccag gatccggatt taaaacctcg atctcaccct
      841 gggaagataa gctcggcaat gctccggcaa gcgggcgccg cgctggacaa aatcaaatgg
      901 acatctgagg acgtagaacg atttatggga atctacctta ccgaacccaa accccatgtt
      961 ttttttaaac gaccctcgca acctgtgaac gagcaagagt tcaccctccg ggccgagcag
     1021 gagggactgg agctcgatct taaaagccgc atgctgtgga aaggatcgaa cttctttctg
     1081 aatggagaat tttgcagggc tggaaacgcc gcaggaaccg tactcaaagc acttgccgat
     1141 catcttgaga ttcagcctgc cgagccgctt aacgagcaag tcctcccgat cctttatcaa
     1201 tggtatctcg acgggtttgt gactttctcg gaaagggtta aaaaactgca ataatgggcg
     1261 tgatcgactc gacgcgttcg taccattgcc tgagctcgtc tgttaacact tgtttcgcgc
     1321 cggcgacggt gacggatttc aactggaacg aagcgcaatc cgcccagcag agcaagcgct
     1381 ataagcggcg agtaacgatg gactgattga aatttcgtgg gcggcggttt ttcaagaaca
     1441 gctttgctgt tccgcccaaa ctctagccct gccaaagttt taaatgcatg gggtataatg
     1501 ctgagctagc gttttttggt catccggaaa accttgtcaa gcgcggcttt gccttgctat
     1561 ttatggctgc gggagacaac gactagacaa catttccgaa ttctgctatt aagatagtgg
     1621 ctattaagat acggtcttct ccgattttga atggccgaaa cccgcttata aaatcattat
     1681 tggttatctt attggttatc agttggtacg ttataaactt cttattaagg agattaaaaa
     1741 catgaagtac tcgctcctcg ctatagcctt ggcaacactg acacttggtg catgtgatca
     1801 aatgaaagag cataaaccgg gtgaaggaaa acccgggcaa tatccgccca gcctttggga
     1861 gaaacgtgaa ggacaggtaa gtgacgctga aatcgaagca gcgcaaaagg ccgacgctga
     1921 agccgctgcc aaacaacaac agcaatccgg cagttcgaca gagccgccaa agtaagccga
     1981 tggatgcgca agccctttaa aaagccgtcc ttcgggacgg ccttttttta gggactctaa
     2041 tagatgggct ccctgctttg tgctacacta ctaccctgga tcaaacagga tgaccgcaca
     2101 atgaaatatt tcccgctcct tgccatctcg ctggctacac taacgctatc cgcttgtgaa
     2161 tataagaagc accccatgga taagccacct ccctccgcca tggctgagcg tgattcagcg
     2221 cctaaatttg atgagctcga taaagaggaa tccaaaggag gggcgggttc cggcgaggcg
     2281 atgacaacag ctcctggtcc cagtgctgct ggtgctcgaa ctgagcctcc tggcaccggt
     2341 gctcccggcg ccatgaccga agctcccaag tagatggcag gaatcccgac catgtggcga
     2401 tagtcgtctt tgcaggcatt cctgtcttgc tttgattttc ggcgaaaaac gaacatttag
     2461 ctggatttcg tcgttttgct gtatactgtc gcctgcatga cctctttaat ttttctagga
     2521 aggaagatct tataatgaaa tattcacttc tcgccgccct tctggtggct ctcagcctga
     2581 cagcgtgtgg gagaccaaaa caggccctgc cggaaaattc gaccacctac ggaacaagaa
     2641 ccgaagaagg cactacgctg gatcaggaac gcggcgctgc agctactgcc ccagccgctc
     2701 cgggcgacaa aaccgccgct gcaccaggcg cgcaggagga tgaatactcc ggtcaggccg
     2761 ttcttccagg acatgcgctt gacggaaatg gcaatccgat aaaggaaggc caaagcgcta
     2821 tcccggcggg ccactaggaa caacagctta gagcaaacct gaaagccatc ctcctggatg
     2881 gcttttttct ttttctttct ctttttgccc aaaaaagtaa taccggttga aaagcaagtg
     2941 aatgtatccc tgcagcaatt cactgtatat cagcatggtg agactttggg tgagccttgc
     3001 tcgcttgaaa aatgaaacaa tttcgatata ctatggcttt ctcaacttct ctctcaatcg
     3061 acactttaaa gggattaaac taatgagact tacgcttgtc gctgctgccc tgaccgtact
     3121 gaccttgact gcctgtggca aaccaagtca agcacttccg gaacaggaaa aggcaaattt
     3181 ttcgaaaata accgggcagg ctagtcccac ttcgggcgct cctgctacgc ctcccgcttc
     3241 cggaaatgcc gccggtggat catcagataa gactgaagga tcgaagtaac atatctggat
     3301 aaaacaggca gctaaataat catagtaatt taaggctgac agttttataa aaaagccgtc
     3361 ccccaaagcc gtcctccgga cggctttttt attgccttgc tttcggctca aataagttga
     3421 cgccagccaa aaccatcgtc ttgctcggcc acgccgatcc agtccaacgt acagttcaaa
     3481 gcactgctca agactgtacg agcaagtagt ggagtattgt ttttctggct caaatgggcg
     3541 gcaatgatat gctgaagacg gctgcaatcc aggctagcga gaaggttggc agaagctgcg
     3601 ttctcgaggt ggccgaagcg gctggcgatg cgctgcttga gcctccacgg gtaatcacta
     3661 ttggcaagca actccgcgtc gtgattacat tcaagcacga gtgcatggca acggcttaaa
     3721 gtcgcttcga tatgcggagt cgaaatcccc gtgtcagtca gcacgcccaa gcgggttgcg
     3781 ccgttgctga agacaaactg ggcgggttcc tgcgcgtcat gcggaacagg gtaaggctgg
     3841 acctcgatat cccctatggc gaagcgctgg tagtcttcta tgattttaat cccgggccat
     3901 ccggcaaaac tcctttccgc gctacgcaag gtaccgtatg tcaaccatat cgggatgcca
     3961 aacctgcggg caaacctcgc gacgcctgcg atatggtcgt cgtgctcatg agttacaaca
     4021 ataccgctga tcacgtcagg ctcaattccg agccgggaca ctcgcataat agtttccttg
     4081 agcgtgaagc cgcaatccag caatactcgc gttttgcccg cttcgaccac aaggccattc
     4141 ccgcgtgaac cactacccag acaggcaaag cgcatgctat cggagagagg aagaaccggc
     4201 ttctacagat actgccgacg agacaaggtg ttcgcagcca agatcaggaa acaagaaatt
     4261 gcgccggcct gagaagagtc ttgcttttca gcaaattggt tatctcggca gccgaaagcg
     4321 gctcggagaa ataataccct tgaatttcat cgcatccccg ctgacgcaaa aaatcaagct
     4381 gctcaatcgt ctcgacgccc tccgccacca cccttacatt aaggctgtgc gccatatcaa
     4441 tgatcgcgcc agttattgcg gcatccgcgg catttgtcgt gacgtcggta atgaatgagc
     4501 gatcgatttt cagcacatca atgggaaaac gctttacgta agcgagactc gaatagcctg
     4561 tgccaaagtc gtcgaccgaa atgcggatgc ccagcttttt caatctattt agtattccga
     4621 tcattttcgt agcatgcatg gccactgacc cttccgtcaa ctccagctcc agaagatcgg
     4681 aatcaatact attctgcctc aaagcccttt ccgtctggag ttcaaactcc agcagttgcg
     4741 gatgcacacc attttcctct atgaatcgga cgacgtcctg acttaaggtg gcctgggaaa
     4801 attgccttcc tgacacgttt accgatactg cgatctctcc aatccccata ttcctccact
     4861 cggcgatttg cttgcacgcg gtttctatca cccatgcgcc aacctgactg atcagccccg
     4921 tctgctcgag gatggaaata aactcatcag gctgcgtaaa accgtagcca ggccgatccc
     4981 agcgcagcag cgcctccacg cctgtgatcc ggccttgggt cagttccact ttcggctggt
     5041 aatgtaggac gaactggttc aattcgaggg ccttgcgaag cgcattttcc tggtccagct
     5101 tttggacggc ccttgcgttc atttcggctg taaaaaaacg gtatgtatct ctgcccgcct
     5161 tcttggagcg atacatggcc gtatcgccat tcttgaccag cgtatccgcg tccatggaat
     5221 ctgaaggata aacgctgatg cccatgcttg ccgtcaggat gacagaagtc ccttcgatat
     5281 taaaaggtag gcgtagcgcc tccctgatct tgcttgctac catccccgca tctcccgatc
     5341 cgtcaggagt aaccagaatg cagccgaact cgtcgccccc cacgcgcgca accgtatccg
     5401 ttacgcgcaa gcattcgagt aaccgcaagc tgaattggcg caacacttcg tctcccgcag
     5461 cacggcccag ggtatcattg atatttttga aattatcgat atccaggtac agtaccgaaa
     5521 caatcctgtg acccgcctcg gcctgcttga ttgtcttcct gagtgattca taaaacagtg
     5581 tccgattggg aagactggta agtgaatcgt aatgcggaag cggccatacc ggctggtgag
     5641 cctccttgcg atgcgaaatg tcatccatga taccgactac ccggtgcacc cccttcgaat
     5701 ccttgactgg aaaggtgcgt gaatggaccc aacgcacgct gttatccgcc aacaacagac
     5761 ggtagtcatg atccatgctg atatcggata aatctctcga ttcatgcatg acccgctgct
     5821 tgtcttccgg atgaatcgca tcaaaaagtt tttccagccg gtcgccactg gtgacccgcc
     5881 tgcccgtcat gttctcccac gaaggattaa cgtagcggaa catctcatcc ttgacatccc
     5941 atatccagaa aatgttggga aggctgactg ccagctgatc gaatagctct tctgtttccc
     6001 gtgaagacgc cgccctcgct actggcctct cactttgctc acctctcaat ccgattgcag
     6061 atcggtgcaa ttctttttga agcaacctga tttccagaac gtcgcgaata tgtgtgagca
     6121 catcgaactg gtcaaacggt ttagcgataa atccttttgc tccagcctgc agggcgcgtt
     6181 tcttattttc tgactgagcg gtagtcacga gtacaggcag cgtttctccg ggtccaatgt
     6241 tctcgatttc cctcaacgcc tccatgacct ggaacccatc cattcttggc atcatcagat
     6301 ccaacaagat gagatcgtag cgatgcgccc ggtgcaaatc gcaaacctca agtggattcg
     6361 ttgtggatga gacagaaaca taaccacccg cccgaagcat gcttttcata actgcaacat
     6421 cggtttcgcg gtcatccacg atcaggatgc tg
//
LOCUS       DASSHJ010000065         6470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_719519, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000065 DASSHJ010000000
VERSION     DASSHJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719519"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFI43_02605"
     CDS             <1..345
                     /locus_tag="VFI43_02605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7061049.1"
                     /translation="RMASLYRCADIMINPSLADNMPISVLEALASGVPVVSTSVGGVP
                     YLVEHGKTAMLVPVQSPEAMANAVLALLNDSSRTKRIREAGIESVQKYTWPNVRARLL
                     GVYSEAMEKRVN"
     gene            565..1917
                     /locus_tag="VFI43_02610"
     CDS             565..1917
                     /locus_tag="VFI43_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7061050.1"
                     /translation="MDLYTSLVSSLVFPLQEQLKKHQSVAIRRGMEATQWWDASRLEE
                     LQVSKLKRFLNEITEHVPYYRTLFSRLGFRAGDIRYLSDLACLPLLDKSTIRSNVEAL
                     KSENARDLARFNTGGSSGEPLVFYISKERVSHDVAAKWRATRWWDVDIGDKEIVVWGS
                     PIELGAQDGLRALRDRIFRTTLLPAFEMTEQRLDGFLRDMRAVRPKMLFGYPSALSHI
                     AAYAESAGQRMDDLGIRVAFVTSERLYDEQRNKITRTFGCPVANGYGGRDAGFIAHEC
                     PSGGMHITAEDIIVEIVDQNGKRVGDGEAGEIVVTHLATRDFPFIRYRTGDMGVLDSK
                     ACACGRGLPLIKEIQGRSTDFVVAEDGKIMHGLALIYVLRELSEIQHFKIVQESLDLT
                     RIYVVSDGMLSPSAAEKIRQGFRKRLGENVTVAIEEVERIPSEKSGKFRYVTSKVAAT
                     "
     gene            2069..3538
                     /locus_tag="VFI43_02615"
     CDS             2069..3538
                     /locus_tag="VFI43_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET7061051.1"
                     /translation="MSNENNEIVDKVLSGARWATGLRLAGQAISWLSTIIVVRFISPE
                     DYGLNAMVEAPLELLALLCTFGLDLALVRSKHIEEHELRSAFGWLLLINGALFLAYFF
                     GSGLIASYFHEPRLEPRAKVLAFIFLLTPFRVIPNALLDRNLKFKLRAAVELVAGVCS
                     AVATLLLALWGAGVWALVIGVLINRILLAIILMILQPWFIMPSLSFSAARGMMIFGGK
                     WSLLGVVVLITDKLATLIGAPILGAEALGIFAVTFQFALLPLAKLMPVINTIIFPAFS
                     KFQDQPDVAAYYLGRALRIISLGLFPIMIGLACVSEGFVATVLGERWSGVILPLALLS
                     SVMPFRMATSLLRPVLGSMGRPDLALKSAVIGFMTLLPLMFIGTHYGVLGMVVALMIS
                     ELIVAFVTIGMSKVVLGTSVTKVAECFSPAIVSSLVMAACLFGIRALSLNGISLGDLF
                     IEVALGALVYFLTLRIFYEKLLQESIKLVLGRRNAGYSA"
     gene            complement(3908..4507)
                     /locus_tag="VFI43_02620"
     CDS             complement(3908..4507)
                     /locus_tag="VFI43_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061052.1"
                     /translation="MNKTFGRVMKTVGIALVALVAWPAHAQLMLAHEGHHSGDCAIKN
                     GAFPVAFSAYEKPKGSLPPMHAFCDHVPEGAGELHLTVDLTDPDSREIPIAVRLVMEG
                     HGEGGHEILSLPAKAYPSGSITFEATFDDPGQYAVLLDTEKDGKMTQAVRIPVHVGGG
                     GGHGSHGSGFGATEIALLVAVAGAAAFFLMRRRTSAKAS"
     gene            4881..5987
                     /gene="nirK"
                     /locus_tag="VFI43_02625"
     CDS             4881..5987
                     /gene="nirK"
                     /locus_tag="VFI43_02625"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HET7061053.1"
                     /translation="MNKAVRGGVIGLGMLGMCSSVAALAATDISKLPVVTQELVAPPF
                     VPKHDQVAKGGPKVVKVRMETIEKQVQIDPSGTKMWALTFNGTVPGPLIVVHEGDYVE
                     LTLVNPKTSTMSHNVDFHASTGALGGAGLTLVAPGEDVVLRWKAVKPGVFVYHCAPGG
                     TMIPFHVISGMNGAVMVLPRDGLKDAKGKPVRYDRAYYIGEQDLYIPKDKDGKYKTYG
                     SPAEGMHEMLELSKGLIPTHVVFNGAVGALTGDNALKAKVGEKVLFIHSQANRDTRPH
                     LIGGHGDLVWVGGKFADPPIQGQETWFIPGGAAGAALYEFRQPGLYVYLNHNLIDAVL
                     LGAAAHMNVEGKWDDDLMKQLKKPNENSTPAMDH"
BASE COUNT         1437 a         1639 c         1868 g         1526 t
ORIGIN      
        1 cgcatggcat cgctttatcg atgtgcggac atcatgatca acccgagcct ggccgacaac
       61 atgccgattt cggtattgga agcgcttgca agcggtgtgc ccgtggtcag cacgagcgtg
      121 ggaggggttc cctatctcgt ggagcacgga aagaccgcga tgctggtgcc tgtgcaaagc
      181 ccggaagcaa tggccaacgc tgtcctcgca ctgctgaacg attcttcgag gacgaaacgg
      241 atcagggaag ccggtatcga gtccgtgcag aaatatacat ggccgaacgt acgcgcccgt
      301 ttactcgggg tttactctga agccatggag aaacgggtaa attgaaaaaa cggaacatcc
      361 tgtccgctca gccaccgatg aaccgatgca tttttagcaa ctgaatgggt gcccgctgga
      421 tcgcggcttc cagcaaatca gctactcatc gaacctttga gccacctgat cgaaatcgcc
      481 caagttcttt tatccccaac atcgagctga acggctttcc ctggcagatc gcaaggtgaa
      541 tcggaaagca gtgatataca ttaaatggat ttatatacct cactcgtttc cagtcttgtt
      601 tttccccttc aagagcagct aaaaaagcac caaagcgtag cgatccggag gggcatggaa
      661 gctacccagt ggtgggatgc gagccgcctg gaggaacttc aagtctcaaa gctcaagcgg
      721 ttcctgaacg agatcaccga acatgtgcct tattaccgta ccctgttttc ccgcttggga
      781 tttagggcgg gggatattcg ctatctttcc gatcttgcat gtttgccact tctggataaa
      841 tcgacgattc gttcaaatgt cgaggcgttg aaatcggaaa acgcacgtga ccttgctcgc
      901 ttcaataccg gcgggtcctc aggtgagccc ctggttttct atatcagcaa agaacgcgtc
      961 agccatgatg tggccgccaa atggcgcgcc acccggtggt gggacgtcga tatcggggac
     1021 aaggaaatcg tggtgtgggg ctcgccgatc gaactcggcg cccaggatgg attgcgcgct
     1081 ttgcgggatc ggattttccg gaccacgctg ctgcccgctt ttgaaatgac tgaacaaagg
     1141 cttgatggat ttctaagaga catgcgcgcg gtccgtccga aaatgctttt cggataccct
     1201 tcagccttgt cgcatattgc tgcatatgca gagtcggcag gccaacgcat ggatgatctc
     1261 ggtattcgcg tcgcttttgt tacatcggag cgcctctatg acgagcaacg caataaaatt
     1321 acccggacat tcggatgccc ggtcgccaac ggttatggcg gccgtgacgc gggctttatt
     1381 gcacacgagt gcccgagcgg cggcatgcat attactgctg aggatatcat tgtcgaaatt
     1441 gtcgatcaga acggcaaaag agtaggcgat ggggaggctg gagagatcgt ggtgacgcac
     1501 ctggctaccc gcgatttccc ttttatccgt taccgcaccg gcgatatggg agtgctcgac
     1561 agtaaagcct gcgcctgtgg cagaggcttg ccgctgatca aggagattca gggtcgcagt
     1621 acggattttg ttgtagcgga ggacggcaag atcatgcatg ggctggccct tatatatgtg
     1681 ctgcgcgagc tatccgagat tcaacacttc aagattgttc aggaaagcct tgatctcacc
     1741 cggatatacg tggtttcaga cggcatgctg agtccatctg cggcagaaaa aatccggcag
     1801 ggattcagaa aaaggctcgg ggaaaacgtt actgttgcaa ttgaggaagt agaaagaatc
     1861 ccctcggaaa aatccggaaa gtttcgctat gttacgagca aagttgccgc aacgtgactg
     1921 agaacacgat atcttgcaag atgatgacga ctgaaagaaa tcccgggagg acaaggatgc
     1981 gatgtgccgg aggaaagctg ccccctgtct ttttttcctt gcccgttcca ggaaaccggc
     2041 ccgtcgcggc aggaaggacg cgacttgcat gagcaacgag aataacgaga ttgttgacaa
     2101 agtcttgtcc ggggcccggt gggcaacggg actgcgcctc gccgggcagg caataagctg
     2161 gctcagcacc atcatcgtgg tgcggttcat cagcccagag gattacggac tgaacgcaat
     2221 ggtggaagca ccgctggagt tgctggcgct tttatgcacg tttggtcttg acctcgcgct
     2281 ggtgcgatcc aagcatatag aggagcatga actgcgtagt gcctttggct ggctgctgtt
     2341 gatcaacgga gctctttttc ttgcctattt ctttggctcc ggccttatcg ccagctactt
     2401 tcatgaaccc cggctcgagc cgcgtgcaaa ggtcctggcg ttcatttttt tgctgacgcc
     2461 attcagggtg ataccgaatg ccctgctgga tcgcaatctg aaattcaagc ttagagcagc
     2521 cgtggaactg gttgccggcg tttgctcggc cgttgctacg ctgttactcg ccctttgggg
     2581 ggcaggtgtg tgggcattgg tcataggcgt actgatcaac cgcatacttc tcgcaatcat
     2641 cctcatgatt ctccagccat ggttcatcat gccttcattg agcttttcgg cggcgcgcgg
     2701 gatgatgatt tttgggggaa agtggtcgct gctcggcgtt gtagtgctga tcaccgacaa
     2761 gcttgccacc ttgatcggcg cccctattct cggtgcagaa gccctcggaa tttttgcggt
     2821 gacctttcag tttgccttgc tgcccctggc caagttgatg ccggttatca acacgatcat
     2881 ttttcccgca ttttccaaat ttcaggatca gccggatgtc gcggcgtact atctcggaag
     2941 ggcgctgagg ataatctcgc tcggtttgtt tccgatcatg atcggccttg cctgtgtatc
     3001 ggaggggttt gtggcgacag tattgggaga aagatggtcg ggagtcatcc tccctctggc
     3061 gctgttgtcg agcgtaatgc cgttcaggat ggcaacatcg ttgctgcgcc ccgtactggg
     3121 cagcatgggg cggcccgatc tggcattgaa gtcagcagtc attggattta tgaccttgct
     3181 tcccctgatg ttcatcggca ctcactacgg ggtgcttggc atggtggtgg ccctgatgat
     3241 tagcgagctc attgtcgctt ttgttacgat tgggatgagt aaggttgttc tagggacttc
     3301 ggtgacaaag gttgcagaat gcttcagtcc cgcaatcgtt tcctcgcttg tgatggccgc
     3361 atgcctgttc ggaatacggg ccctgtcctt gaacgggata agccttggcg atttgtttat
     3421 tgaagtcgct ctcggggcgc ttgtttattt tctgacactg cgaatttttt acgaaaaatt
     3481 gctgcaggaa tcgatcaaat tggtcttggg cagacggaac gcaggctatt ccgcataaaa
     3541 tcgtagttcg acccgctgtt ttcgtgtaaa cgaaaacccc ctcaccccgt tcgtttcgga
     3601 aaatattcaa taaaatgctg gcgtcgggag gctcgagcgg acagcacgtg ttgccaggga
     3661 tttgctgcgc gccttatttg gagcaggaat gcctggatga atatccagac ggtcacatct
     3721 gcagggaggt gtagcaggat tttttactat ctaccgacgc tctcaagcgc ttgctgcaag
     3781 cgcccggctt ccgtatcaat gaccgggcgc tcctggagaa gttttggaag tttcggaatt
     3841 ggattatctg cgtaaccttg atgaagtagt tgaccaacca gaaggggaag aaactaaatc
     3901 agcactgtca ggaggccttt gcgctggttc ggcgacgcat caggaagaag gctgccgcac
     3961 ctgccaccgc aaccagcagc gcgatctcgg tcgctccaaa acccgaacca tggctgccat
     4021 gccccccgcc tccccccaca tgtaccggga tgcgtaccgc ttgcgtcatc ttcccgtctt
     4081 tttcggtatc gagaagcact gcgtactggc cggggtcgtc aaaagttgcc tcaaaagtga
     4141 tgctgccaga tgggtatgcc ttggctggaa gcgacaaaat ttcgtgtcca ccctctccat
     4201 gtccttccat taccagacgc acggcgatcg ggatttccct ggaatcagga tccgtcaagt
     4261 cgacggtgag gtgcaattca cccgcccctt caggcacgtg atcgcaaaat gcgtgcatgg
     4321 gaggaaggct tccctttggc ttttcataag cgctgaacgc tacgggaaac gctccgtttt
     4381 ttatcgcaca atcccctgaa tgatgacctt catgagccag catcagctgc gcatgggctg
     4441 gccacgcgac caacgccacg agtgcgatcc caacggtttt cataacccgc ccaaacgttt
     4501 tgttcatact ctctccgcaa taaaagtccg atattatgca ttccagcata atgtataacg
     4561 acggattgta taacaaacgc aggtcaaaat gaaaacatcg tatgacggca taacaggaac
     4621 ggatgagctt ctgcgagctg gcgtccatat cgaggcaata tgtcattgaa tttttcccgg
     4681 ttacgccctg ccgcgcctgg cgctaggctc caggccaagc cccataacgg ttttcgccgg
     4741 gccaacgccc tagcctggct gaataattct tcatggacat gcaaattgta acgcggtata
     4801 gttgccgagc cttcgctaca tggatcgagc agatgaagtg ttgcatgatg ggtgtgaata
     4861 acattacatg gagggaaaag atgaataaag cagttcgagg gggggtaatt ggactgggga
     4921 tgcttggcat gtgcagcagt gtcgctgcgc tggcggcgac cgacatatcc aagttacctg
     4981 tagtgaccca ggagttggtt gcgccgccat ttgtgcccaa gcacgatcag gtggccaagg
     5041 gcgggccgaa ggttgtcaag gttcggatgg agacgataga aaagcaggtt cagattgatc
     5101 cgagcggcac gaagatgtgg gcgttgacgt tcaacggcac ggttcccggg ccgctgattg
     5161 tggtgcatga gggtgactat gtggaactga cgctggtcaa tcccaagacc agcaccatgt
     5221 cgcacaatgt ggactttcat gcatcgacgg gtgcgctggg gggagccggg ctgaccctgg
     5281 tcgcgcctgg cgaagacgtc gtattgcgct ggaaagccgt caagcctggc gtatttgtct
     5341 atcactgcgc cccgggcggg accatgattc ctttccacgt catctcgggc atgaatggcg
     5401 ccgtcatggt gttgccgcgc gatggcctga aggacgccaa gggcaagccg gtgcgctatg
     5461 accgtgccta ctatattggc gaacaggatc tttacattcc gaaggacaag gacggcaaat
     5521 acaagaccta tggctcgcct gccgaaggca tgcatgaaat gctcgaactg tccaaggggc
     5581 tcatcccgac ccatgtcgtc ttcaatggcg cggtgggtgc tctgacgggc gacaatgcac
     5641 tcaaggccaa agtgggcgag aaagtattgt tcattcactc ccaggccaac cgtgatacac
     5701 gtccccacct gattggcggc catggcgacc tggtatgggt gggcggcaag tttgccgatc
     5761 cgcccataca aggacaggaa acctggttta ttcctggtgg agcagcgggt gcggccctgt
     5821 atgaattcag gcagccgggc ctgtatgtgt acctgaacca caacctgatc gatgcggtat
     5881 tgctgggtgc ggcggcgcac atgaatgtcg aaggcaagtg ggacgatgat ctcatgaagc
     5941 aactcaagaa acccaacgag aattcaaccc cggcaatgga tcactaacag caacatccgg
     6001 agcaggctcc ataagggctt taaactccat caaacctaaa agcggcttca acaaacgttg
     6061 aagccgcttt tttcttatga ttttcctctt ggcttgggga gtcgtttctc tcctttccca
     6121 agtcataacc tcctgatata aaggacccgt tctcgaacca ggttcaatct ccttgacaca
     6181 gccgtatgta gcgggttagg ctaggcgggt tcgggttggc cctatgggga tacctggacg
     6241 ggtactgggt atgagggagt ggagagtctg cctgaggtat cagggtgtgg ttgaggaagg
     6301 gcgggcggga tacctggtat tgagaacgaa caactattac ggaacgaagg agaagaagcg
     6361 atgttattga agccctggct gaggggagct gtggcggggt tgggggtttt gtttgcgtcg
     6421 atggcgcagg cggactttcc gagcgtgccg aaggagacgt acaaggcgct
//
LOCUS       DASSHJ010000066         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_393275, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000066 DASSHJ010000000
VERSION     DASSHJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393275"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFI43_02630"
     CDS             <1..701
                     /locus_tag="VFI43_02630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7061054.1"
                     /translation="HPNVHCFPSSVDVDHFSQALNRSISHPGHDRIPKPRLGYYGVID
                     ERLDLSLIAQLADSHPEWQLMLVGPIAKIEKIELPRRQNIHYFGQQSYEELPRILASW
                     DVCLLPFARNESTRFISPTKTLEYLAAQLPVVSTDIADVVELYGKAVSIAATSAGFVS
                     ACEKVLGESVEERSQRIRAADSILSSGSWDKTAGKMQELLSALPQRASVSIGPSRPKN
                     CASGTPAGGNHKFH"
     gene            992..1606
                     /locus_tag="VFI43_02635"
     CDS             992..1606
                     /locus_tag="VFI43_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HET7061055.1"
                     /translation="MARSSEPRHVGFIPDGNRRWALSKGLQKQEGYAAGILPGILLME
                     RCREMGIVEASVYCFTQDNTKRPRDEKIAFTNAAVSFASEIARRNIALLVVGDETSAQ
                     FPNALKPFRERQGIGMKVNLLVNYGWEWDLDGLKTGALRSSDVSRIDLIVRWGGGRRL
                     SGFLPAQSVYADFYVRDEYWPDFELSHFDHALAWFRKQDRTFGG"
     gene            complement(1852..3681)
                     /locus_tag="VFI43_02640"
     CDS             complement(1852..3681)
                     /locus_tag="VFI43_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061056.1"
                     /translation="MFAFFEQLVRPTVVPPKSPPSGLLAFYWHFVSQTKGVYAALFAT
                     GLTVALIDTLIPVFIGQLVDLMEATDRAKALKTATPTLVGMLVLVLIGKPLAILIDSF
                     IRHNAVVPGVSSLIRWQSHWHVIRQSWGLFQNDLAGRIASRVMQTSDAVRECVVSSIH
                     AIWYITVYWISSLWLMASADWRLAVPTAIWLVAYIFFLYYFVPRMRDLSRKSSEQRSV
                     LMGRVVDSYTNILTVKLFSRTSDEDAYVRDAINDHKGAIAAHMRLVTKFSTTLSALNA
                     LLLVTTTAIGILLWARGDVEAGTVAMVVPLAWTIANAGGWVSWEMNGVFENMGVVQEG
                     MQTIAVPNALPDTPGARDLDAADVRGEIRFERLNFTYGRSDGRKVLDNLSFTIRPGER
                     VGLVGSSGAGKSTLISLLLRFHEIRSGSIRIDGHDTRELTEESLRHSIGVVAQDTSLL
                     HRSIAENIAYGCPGATRAEIEAAARRAQAHEFIMRQRDDKGRTGYDAHVGERGAKLSG
                     GQRQRIAIARVVLKNAPILVLDEATSALDSEVEASIQEQLLDVMQGKTVIAVAHRLST
                     IACLDRLLVLNEGRVVEEGTHEELLRLDGQYAMLWRHQSGGFL"
     gene            complement(4123..5952)
                     /locus_tag="VFI43_02645"
     CDS             complement(4123..5952)
                     /locus_tag="VFI43_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061057.1"
                     /translation="MFAFFEQLVRPTVIPSKSPPSGLLAFYWHFISQTKSLYAALFAT
                     GLTVALIDTLIPVFIGQLVQLMEATDRISALKAATPMLTGMLVLVLIGRPLAILIDSF
                     IRHNAVVPGVSSLIRWQSHWHVIRQSWSLFQDDFAGRIANRVMHTSDAVRECVVSSIH
                     AVWYIGVYAVSSLWLMASADWRLAVPTVLWFGAYILFLYYFVPRMRDLSKTSSEQRSV
                     LMGRVVDSYTNILTVKLFSRMGEEDAYVRDAINDHTGAIAAHMRLITKFTTTLAVLNA
                     LLLVGTTAIGITLWAQGNLEAGTVAMAVPLAWTIANAAGWVSWEMSSAFENIGVVQEG
                     METIAVENSLPDIPAARNLDVSSVHGEIRFERLDFTYGRSDGRKVLDSLNCTIRPGER
                     VGLVGSSGAGKSTLINLLLRFYEIESGSIRIDGHDIRELTQESLRRSIGVVAQDTSLL
                     HRSIAENIAYGSPGATPAEIEAAARRAHAHEFIMRQRDDKGRTGYDAHVGERGVKLSA
                     GQRQRVAIARVVLKNAPILILDEATSALDSEVEAAIQDQLLDLMEGKTVIAIAHRLST
                     IACLDRLLVLNEGRIIEEGTHEELLRHDGQYAMLWRHQSGGFL"
BASE COUNT         1376 a         1722 c         1640 g         1308 t
ORIGIN      
        1 gtcatcccaa cgttcactgc tttccaagca gcgtcgatgt cgaccacttt tcacaagcat
       61 tgaaccgcag catcagtcat cctggacatg atcgcatccc caaacctcgg ctggggtatt
      121 acggagtgat tgacgaacgg ctggatctga gtcttatagc tcaacttgct gattcgcatc
      181 ccgaatggca actgatgctg gttggcccaa ttgctaaaat tgaaaaaata gagctgccga
      241 ggcgtcaaaa cattcattat ttcgggcagc aaagttatga agaattgccg agaatactgg
      301 ccagttggga cgtttgtctt ctcccattcg cccggaatga gtcgactcgc ttcatcagcc
      361 cgaccaagac acttgaatac cttgccgccc agttgcctgt ggtaagcact gatattgcgg
      421 atgtagtgga actttatggc aaggccgttt ccatcgcagc tacatcagcc ggatttgttt
      481 ctgcctgtga aaaagtactg ggtgaatcgg tcgaggagag gtctcaaagg atcagggcag
      541 ccgacagcat attgtcctcc gggtcatggg acaaaacggc tggaaaaatg caggagctat
      601 tgtcggctct gccccagcgg gcgtctgtga gcatcgggcc atcccggcca aagaactgtg
      661 cttctgggac gccggcggga ggtaaccata aattccattg atgaggccag agcttgaact
      721 ttctgccgca tgttggcgtg cataagaccg ggaaactgca aagccatgga aaacaaaacc
      781 ttttccatac ctgcccaatc ggctgcctgt tgctggtttt gtcgcatcat cgcgccggag
      841 cgcgacccgg gggctgaagg atatgatcgc gggtctgcgg tagatcatag gcgtgcacaa
      901 gcagaaaacc gcgagaggtg taaaaagccc attgccagaa cttacccccc atgcaggctt
      961 gtgaagtatc accaacaaaa aaggttgccc gatggctaga agtagcgagc cccgtcacgt
     1021 cggcttcatt cccgacggaa accggcgctg ggcgttaagc aaaggcctgc aaaaacagga
     1081 aggatacgca gccggtatcc tgcctggaat attgttgatg gaaagatgcc gcgaaatggg
     1141 gatagtcgag gcgtctgtgt attgctttac tcaggacaat accaagcggc cgcgagacga
     1201 aaaaatcgca tttaccaacg cggctgtttc atttgcttcc gagatagcgc gccggaatat
     1261 cgccttgctt gtcgtgggcg acgagacttc cgcgcagttt cccaacgctc tgaaaccttt
     1321 cagggaacgc cagggtattg gcatgaaagt gaatttgctg gtgaattacg gctgggaatg
     1381 ggatcttgat gggcttaaaa caggagcgct tcgctccagc gatgtctccc gcatagacct
     1441 cattgtgcga tggggaggag gacgccgctt gagtggcttt cttcctgccc agtcggtcta
     1501 tgctgatttc tatgtccgcg atgagtactg gccggatttc gagctgagcc acttcgacca
     1561 tgccttggcc tggttccgaa agcaggaccg gacgtttggt ggttgaccac tagcagaatt
     1621 tcaacaactg ccattaattg atccattttt tacctctccg gccctctccg atcttcagtt
     1681 cacatcttcc ttgcggaaga cgctggtcag gctacccgcg gcgctgaatg cgtttatcgc
     1741 ccgataatct cggtaccccg gaaactgaaa ctgacccatc tttcccccac ctctctccgt
     1801 tctggcgaga gaggggaaga ggcaaattgc cttgctcgct cgctcattgg attatagaaa
     1861 tccacccgat tgatgacgcc acagcatcgc atactgtcca tccaatctca gcagttcttc
     1921 atgcgtccct tcctcgacga ctcgaccctc attgaggaca agcagccggt ccaggcaggc
     1981 aatggtggac agacgatgcg ccacggcaat caccgtcttg ccctgcatca cgtcgagaag
     2041 ctgctcctgg atcgaggcct cgacttcgct gtccagcgct gaagttgcct catccagaac
     2101 aaggattggc gcgttcttca gcacgacgcg cgcaatcgca atgcgctgcc gctgcccccc
     2161 gctgagcttc gcgcctctct cccctacatg agcgtcatac ccggtacgcc ctttatcgtc
     2221 tctctgccgc atgatgaatt cgtgtgcttg tgcgcgccga gctgcggctt caatttcggc
     2281 ccgtgttgcc cccgggcatc cgtaggcaat gttttcagca atcgagcgat gcaacagaga
     2341 ggtatcctgc gcgaccactc caatcgagtg gcgcaagctt tcttcggtca gctcacgcgt
     2401 atcgtgcccg tcgatccgga tgctgccgga tctgatctcg tggaagcgaa gcaacaggct
     2461 gatcagtgtt gatttccccg ctcccgagct tccaaccagt cctacccgtt cgccgggccg
     2521 aatcgtaaag ctcaagttat ccagcacttt tcgcccatcg ctgcgtccat aagtaaaatt
     2581 caggcgttcg aagcgaattt caccccgtac atctgcagca tcgagatcgc gtgcacccgg
     2641 ggtgtcaggc agcgcattgg gcaccgcaat tgtctgcatg ccctcctgta ccacccccat
     2701 gttttcgaag acgccgttca tttcccagct cacccacccg cccgcgttgg cgatggtcca
     2761 ggcgagaggc acaaccatcg cgaccgttcc cgcctcaaca tccccgcggg cccagagcaa
     2821 aataccaata gcggtcgtgg taacgagcaa caacgcgttg agcgccgaca aggtagtcga
     2881 gaacttggta acgagacgca tatgcgcagc aatcgcaccc ttatgatcgt tgatcgcgtc
     2941 ccgcacgtac gcatcttcgt cgctcgttcg cgagaagagc ttgactgtca gaatgttcgt
     3001 gtagctatcg actacccggc ccatcagtac agaccgctgc tcgctgcttt tcctggacaa
     3061 atccctcatt cgaggaacga aatagtataa gaagaagata tatgcgacca gccatattgc
     3121 ggtgggcaca gccaggcgcc agtcggcgct ggccatcagc cataacgacg atatccagta
     3181 gacggtgata taccagatgg catggatgct cgatacgacg cactctcgca ctgcgtcgct
     3241 tgtctgcatg acacggcttg caatgcgtcc cgctagatcg ttctgaaaaa gcccccagct
     3301 ttggcgtatc acgtgccaat gactctgcca tcgaataagg ctgctcacgc ctggcacaac
     3361 ggcgttatgg cggatgaaac tgtcgatcag tatggccagc ggcttgccga tcagcacgag
     3421 taccaacatt ccaaccaggg ttggcgtcgc ggtctttaac gccttggcgc gatcagttgc
     3481 ctccatcaga tcgacgagct gcccgataaa gaccgggatc agcgtatcga tcagcgcgac
     3541 tgtcaggcca gtggcgaaca acgccgcata aacgcctttc gtctggctca caaaatgcca
     3601 atagaatgca agaaggcctg aaggcggtga cttggggggt accacggtgg gtcgaacaag
     3661 ctgctcaaag aatgcaaaca tatgcaaagg gagtttgaag ttattgtcgt aaatacatgc
     3721 tgaaaaattt ccggatatca caacgcccgg gctcttgctg ttcggcaggt tcagtccaga
     3781 ggcacattga actgaccctg ctcgatgcgc gacgacaata acagatggtt gtggagcgaa
     3841 atgccgggag ttacgaccaa aatagtctta tgcttcgaag attgccccta aagcaaaagc
     3901 aggttttttg gcaaagaagt gacgtggaat gggattggga tgaaaagtgg attacggagt
     3961 gaatcctctg gccgcacggc tgggaattgc gactagccag gtacaggcgc cttatactgt
     4021 tcgaaagccg ctattcgggc atggatcaga aagtcgctat ccggcatgcg gtcaataggc
     4081 aagacaagca ggggcgccca taaagctgtc gcgccctcat gattaaagaa agccgccgga
     4141 ctggtgtcgc cagagcatgg cgtattgtcc gtcgtgcctt aaaagttctt catgtgttcc
     4201 ttcctcgata atacggccct cgttgaggac aagcagccgg tcgaggcagg cgatcgtgga
     4261 cagccggtgc gcaatggcga tgacggtctt gccttccatc aagtcaagca actggtcctg
     4321 gatggcagcc tcgacctcgc tgtccagcgc cgaggttgcc tcgtcaagga tcaggatagg
     4381 ggcattttta agtacgacgc gcgcgattgc aacgcgctgc ctctggcccg cgctgagctt
     4441 cacgccgcgt tcacccacat gggcatcgta gcccgtgcgg cctttatcat ctctctggcg
     4501 catgatgaac tcatgcgcat gcgcccgccg tgcggcagcc tcaatttccg ccggtgtcgc
     4561 gcccggactt ccataggcga tgttttcagc aatggaccga tgcaacagag aggtatcttg
     4621 ggcgacgacg ccgatcgagc ggcgcaggct ctcctgggtc agttccctga tgtcgtggcc
     4681 atcgatacgg atgctgccgg attcaatctc gtagaaccgc agcaacaggt tgatcaaggt
     4741 cgactttccg gcgcccgaac ttccaacgag gccgacgcgt tctcccggcc ggatagtgca
     4801 attgaggcta tccagcactt tgcgcccatc actgcgccca taggtgaaat cgagtcgttc
     4861 gaagcggatt tccccatgga cgctgctgac gtcaaggttt ctcgccgcag gaatatccgg
     4921 gagcgaattt tccaccgcga tggtttccat gccttcctgc acgaccccta tattctcgaa
     4981 ggcgctgctc atctcccagc tcacccaccc ggctgcattg gcgatcgtcc aggcaagagg
     5041 cactgccatg gctaccgtcc cggcctcaag gttgccttga gcccagagcg tgatgcctat
     5101 agccgtggtt ccaaccagca gcaaggcatt cagtaccgcc agcgtggttg taaacttggt
     5161 aatcagccgc atgtgcgcgg cgatggcgcc ggtatgatca ttgatagcat cgcgtacata
     5221 ggcatcctct tcgcccatgc gggaaaagag cttcacagtc aatatgttcg tgtagctatc
     5281 caccacgcgg cccatcaata cagagcgttg ctcgctgctt gtcttcgaca ggtcgcgcat
     5341 ccgcggaacg aagtagtata aaaaaaggat ataggcacca aaccacagga cggtcgggac
     5401 cgccaaccgc cagtccgcgc tggccatcag ccacaaggat gagactgcgt acactccgat
     5461 ataccatacc gcgtggatgc ttgataccac gcactcccgc acggcgtcgc tggtgtgcat
     5521 cacccggttt gcgatacgcc cggcaaagtc gtcctgaaaa agggaccagc tctgccggat
     5581 gacatgccaa tgactctgcc agcggatgag gctgctcacg ccaggcacca ctgcattatg
     5641 gcgaatgaag ctgtcgatta gtatggccag tggccttccg atcagcacca gcaccagcat
     5701 tcccgtcagc atgggagtcg ccgccttcaa cgcgctgatc ctgtcggtgg cttccatgag
     5761 ttgaacgagt tgtccaataa aaacaggtat cagcgtatcg atcaacgcaa cagtcagtcc
     5821 ggtggcgaac agcgcggcat aaaggctctt ggtctggctt atgaaatgcc agtaaaacgc
     5881 gaggagtcct gacgggggtg acttggaggg tatgacggtg ggacgaacaa gctgctcaaa
     5941 gaatgcgaac atatgtttag gaagtccgag gttgtttggt taagagcacg ggcacttcac
     6001 cgtagccacc gcattaacgc aagccatccc tcccgggacg acacgc
//
LOCUS       DASSHJ010000067        10689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_172860, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000067 DASSHJ010000000
VERSION     DASSHJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172860"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFI43_02650"
     CDS             <1..325
                     /locus_tag="VFI43_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7061058.1"
                     /translation="FAEKNGYSIVREFCGHGIGTNFHEEPQVLHYGKANTGLTLEAGM
                     IFTVEPMINAGKAPIRQMADGWTIVTKDHSLSAQWEHTVLVTDNGYEVLTLSAGAPAK
                     PAFRA"
     gene            328..3003
                     /locus_tag="VFI43_02655"
     CDS             328..3003
                     /locus_tag="VFI43_02655"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET7061059.1"
                     /translation="MTRSSPPEGPLPIEAARTELLEGRVRLRQRYCETHNSRLLLRDH
                     ANLIDQLLRKAWQDMSMPDSIALIAVGGYGRLELFPHSDIDLLVLLPKMEGAGGQLGT
                     ETRMRLEHWVRFLWDIGLEIGHSVRTVPECIEEAAKDITVQTSLLEARPLAGSQKLFR
                     EFSREMQVNLEPRTFFAAKQLEQQQRHGRYRGATYNLEPNLKEGPGGLRDLQNVLWVS
                     RAAGLGKSWRDLAKRGFITQREAQLAQRHETVLRDLRICLHYLSGRREDRLVFDYQTP
                     LADELGIAAKPPRRADEVMMQRYYRAARGARQINTILLVTIHAELFPNTSEAPVIINE
                     RFQKRGQLLEARHENIFCEHPGAIFESVLLLQQNPELKARSAATLRAMWRAAPLIDSR
                     FRREPRHRQMFMQILRQPRGLTRELRLMNRFGILGRYLPAFGRIVGQMQHDLFHVYTV
                     DEHILMVIRNLRRFMAPEFAHEYPLCTDLINNFERPEVLYLAGLFHDIAKGRHGDHSA
                     LGKEDARRFCGQHGMLSEDVELVAWLVENHLVMSMTAQKKDISDVNVIEDFASRVASE
                     RRLIALYLLTVADIRGTSPKVWNAWKAKLLENLFWATRLRLSGHATDAGTALRNRKDE
                     ALELLQPDGVPREAHERLWSALDSTYLLLHEPEEIAWHTRHLRQHVKPSRPIVRARLA
                     PSGSGIAVLVYTADQKDLFARICGFFDHIDYNIVQAKIHTTRHGYALDTFLVLDSLEL
                     VLDPLDLAERHRDMSGYIQHELARQLEQREPLQPPLRGRLSRQLKHFPMKPEVDIELD
                     EKGMFYILSIVAGDQAGLLSRVAQVLVQFGVDVHSARINTLGDRAEDTFLITGQPLSD
                     AKTLIRLETKLVKALETSDPSNGKPAALLIREEVA"
     gene            complement(3124..5433)
                     /gene="metE"
                     /locus_tag="VFI43_02660"
     CDS             complement(3124..5433)
                     /gene="metE"
                     /locus_tag="VFI43_02660"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET7061060.1"
                     /translation="MALTHNLGFPRIGARRELKHALEAYWRGEISEDRVQAAAAELRK
                     KHWSIQHGAGVDLVPVGDFALYDHVLNMTALLGAVPPRFGPPRDCDIGLDLYFAMARG
                     TAHQPAMEMTKWYDTNYHYIVPEFSADTVFRIASSRLFDEVAEVQALGARPKVVLIGP
                     VTYLHLGKEVGPGFSRLNLLPRLLPVYRDILARLATAGVEWVQIDEPSLGLDLSEEWL
                     RALEQAYVYLHQSIESRPRLLLTTYFEAVDNHAGRLKNLPVEGLHIDLSRAPGQLAAF
                     IKDYPVDKILSLGIIDGRNVWRADLAQAFSILSEAQSILGERLWLAPSCSLLHSPVDL
                     ELEMRLEPEIKDWLAFSVQKLDEISILGRGLNQGEETIREQLDAATRARQARARSPRI
                     HSPVVQERLQHLTWRDSQRASPFPERRLLQRKRFQLPLLPTTTIGSFPQTPEIRRVRA
                     ALKKGEASNLQYLESIRNQIVLAIKRQEEIGLDVFVHGEAERNDMVEYFGEQLWGYAF
                     TENGWVQSYGSRCVKPPILYGDIYRPEPMTVDWIRYAQSQTEKPVKGMLTGPVTMLMW
                     SFVRDDQPRPVTALQLALAIRDEVFDLEQAGISMIQIDEPAFREGLPLKKASWEAYLD
                     WAVKAFRVASSGVKNDTQIHTHMCYSEFNDILPAIAEMDADVITIETSRSRMELLKGF
                     AEFKYPNEIGPGVYDIHSPRIPETVEMVELLEKACLVIDPEKLWVNPDCGLKTRTWEE
                     TSTALKRMVAAAGKMRARLQARELAEQSV"
     gene            5580..6509
                     /locus_tag="VFI43_02665"
     CDS             5580..6509
                     /locus_tag="VFI43_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7061061.1"
                     /translation="MSLASILELRHLRSLMALHETGSVSRAARRVHLTQSALSHQIKA
                     LQDHYGLPVVQRKGQSVELTDAGKQLVTLAEKVLGEIQATERDIAKLSQNVSGSLRIA
                     LECHTCFDWLMPLMDEFRQHWPDVELDLVSGFHSDPVKLLEEGKADVVIGSESKPCRG
                     VVHEPLFRFEILAVLATEHPLSAKRILHPTDFASETLITYPVPEDRIDLILQVLRPAG
                     IHPQRRTAELTVAILQLVASRRGVAALPSWGIKNYLDHDYVIARRIGAQGLWSDLYAS
                     TLKETASRAYMRDFLTTARNTCFATLEGIIPLE"
     gene            complement(6695..7444)
                     /locus_tag="VFI43_02670"
     CDS             complement(6695..7444)
                     /locus_tag="VFI43_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HET7061062.1"
                     /translation="MKTNVSLKQKIDSIIDARHLLKHPFYVAWTEGKLSKEQLRHYAE
                     QYFHNVLAEPTYLSAVHFNTPHFHSECNSGDISVRQEVLKNLISEEHGEKNHPALWKS
                     FALALGSSDSSLANATALPTTARLVSTFRDICLNEPFYAGLAALHAFESQVPSIAAVK
                     IDGLARFYGMSNPDSYEFFSVHQEADVYHSQAEWALIERFADTPEKQAEVVAATARAC
                     DALWGFLDGIYEKYCANLACEEEEEAAVTLH"
     gene            7669..8091
                     /locus_tag="VFI43_02675"
     CDS             7669..8091
                     /locus_tag="VFI43_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HET7061063.1"
                     /translation="MDEQSSLFALFTSSFLAATLLPGGSEAVLFGVLTAYPQLYWPAL
                     GVATLGNTLGGMSSYAIGRLLPDEKALEKKLGGNLRGIEWVSKHGSPVLLLSWVPLIG
                     DLLCIAAGWLRINWVLATLFMALGKFGRYWAIGAAMNI"
     gene            complement(8288..8617)
                     /gene="grxD"
                     /locus_tag="VFI43_02680"
     CDS             complement(8288..8617)
                     /gene="grxD"
                     /locus_tag="VFI43_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET7061064.1"
                     /translation="MDTQEVIKEQITTHPVVIYMKGTPQAPQCGFSAHAVKMLNACGV
                     NNLFAVDVLAEPEFRQGIKEYSHWPTIPQVYINGEFVGGSDIVAEMYQSGELQKLIQE
                     QKDKAPS"
     gene            8794..8928
                     /locus_tag="VFI43_02685"
     CDS             8794..8928
                     /locus_tag="VFI43_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061065.1"
                     /translation="MVKSTRREYLETIRKRYRRACKKDKKPILDEFCVNCGYHRKYAI
                     "
     gene            complement(9261..10103)
                     /gene="prmC"
                     /locus_tag="VFI43_02690"
     CDS             complement(9261..10103)
                     /gene="prmC"
                     /locus_tag="VFI43_02690"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7061066.1"
                     /translation="MITVHEALCQAQGIIDNTDARMLLQHVLKLNLAHMIAHSEQELS
                     VEQAQQFAELLGRRRQGEPVAYLTGEREFYSLDFEVTPAVLIPRPETELLVDLALERV
                     PVNRAITILDLGTGSGAIALAIAKQRPLARVVAVDISPEALIVARSNAMQLNVDNVRF
                     MQVDWFNGLAGERFDLIVSNPPYVANGDSHLTQGDLRFEPSIALTDGGDGTRCLRSII
                     ASAPAYLMPGGMIMVEHGYDQGFRCCQLLNEAGFHDVFSHPDLAGIMRVSGGVAYHFS
                     LENE"
     gene            complement(10235..>10689)
                     /locus_tag="VFI43_02695"
     CDS             complement(10235..>10689)
                     /locus_tag="VFI43_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HET7061067.1"
                     /translation="ADEIMDVVLNPAELRIDTFRASGAGGQHINKTDSAVRITHLPTN
                     TVVECQDGRSQHKNKAQAMSILAARIRDRQMQEQQSKQAATRKSLVGTGDRSGRIRTY
                     NFPQGRVTDHRINLTLYKIEQIMDGDLNELCSVLMSEHQAEQLAAMGE"
BASE COUNT         2543 a         2914 c         2815 g         2417 t
ORIGIN      
        1 atttgccgaa aaaaatgggt acagcattgt aagggaattc tgcggtcacg gcatcgggac
       61 aaacttccat gaagagccac aggtacttca ctatggaaaa gccaatactg gactaacact
      121 tgaggcagga atgattttca ccgtggaacc aatgatcaac gcggggaaag cccctatcag
      181 acagatggct gatggctgga ccattgtaac gaaggatcac agtctttccg cccagtggga
      241 acatacggta ttagtgactg ataacgggta cgaggtgctg acgctctcgg caggggcccc
      301 tgcaaagccc gcctttcgtg cctgaaaatg acccgctcca gcccccccga aggcccactg
      361 ccaatcgagg ctgcaaggac agagctgctc gaaggccgag tgcggttgcg ccagcgttat
      421 tgcgaaactc acaatagccg cctcctgctg agggatcacg ccaacctcat cgatcaactc
      481 cttcgtaagg catggcagga catgtcgatg cctgactcca ttgcattgat agcagtggga
      541 ggatacggcc ggctggagct gtttccgcac tccgatatcg atttgctggt gctactccca
      601 aaaatggaag gcgcgggcgg ccaactgggt accgagacca ggatgcggct ggaacactgg
      661 gtgcgctttt tatgggacat tggcctggaa atcgggcata gcgttcgtac cgttccggaa
      721 tgtattgaag aagcagcgaa ggacataaca gttcagacca gcttgctgga agctcggccc
      781 ctcgcaggtt cgcaaaagct attcagggaa ttttcgaggg agatgcaggt gaatctcgaa
      841 ccacgcacct ttttcgccgc aaagcagctt gagcagcaac agcgtcacgg cagataccgg
      901 ggcgcaacat ataacctgga acccaacttg aaagaggggc ctgggggcct gcgggattta
      961 cagaacgtcc tatgggtcag ccgagcggcg gggcttggaa aatcatggcg tgatcttgct
     1021 aaacgcgggt ttatcactca acgcgaggca cagcttgcgc agcgccatga aaccgttttg
     1081 cgagaccttc gcatttgtct tcattatctt tccggaagac gtgaagatcg ccttgtattt
     1141 gattatcaga ctccccttgc cgatgagctt gggatagccg caaaaccgcc gcggcgcgcc
     1201 gatgaggtga tgatgcagcg ctactaccgt gcggcacggg gcgccaggca gatcaatacc
     1261 attcttctgg tgacaatcca tgctgagctg tttccgaata cgagcgaagc gcctgttatc
     1321 atcaatgagc gattccagaa gcgaggtcag ctgctcgaag cgcgacacga gaacattttc
     1381 tgcgagcatc ccggagccat attcgagagt gttctgctgc tgcagcagaa tcccgagctg
     1441 aaagcgcgga gcgctgcaac cctgcgtgcg atgtggcggg cggcgccgct catcgattca
     1501 agattccggc gcgaaccccg ccatcgccag atgttcatgc aaatcctgcg ccagcctcgc
     1561 ggattgaccc gtgaactgcg gctgatgaac cgcttcggga tcctgggacg atatttgcct
     1621 gcgtttgggc gcatcgtcgg acagatgcag catgacctat tccatgttta cacggttgat
     1681 gagcatattc tgatggtcat caggaatctt cgccgtttca tggcgccgga atttgcgcat
     1741 gaatatccgc tgtgcactga tctcatcaat aattttgaaa gacctgaagt gctttatctc
     1801 gccggactgt ttcacgatat tgcaaagggg cgtcacggag accactccgc gctgggcaag
     1861 gaggatgcgc ggagattttg cggacagcac ggcatgctct ccgaagatgt cgaactggtc
     1921 gcgtggctgg tggaaaatca tcttgtgatg tcgatgacag cgcaaaaaaa agacatatcc
     1981 gacgtgaatg tgattgagga cttcgcgagt cgcgtagcga gcgaacgccg ccttattgcc
     2041 ctttatctgc tcaccgtcgc ggatattcgc ggcaccagcc ctaaagtatg gaatgcctgg
     2101 aaggccaagc tgctcgagaa tctgttctgg gcaacacggc tcaggttgag cggccacgca
     2161 acggatgccg gaacggcttt acgcaatcgc aaggacgagg ccctggagtt gctgcaacca
     2221 gacggagttc ctcgggaggc gcacgagcgg ctatggtccg cgctcgattc gacttatctt
     2281 ctcctgcacg agcccgaaga aatcgcctgg cacactcggc atctgcggca gcacgtcaag
     2341 ccatcccggc ccatcgtgcg agcacgtctg gcgccgtccg gatcgggaat cgcagtgctg
     2401 gtctatacgg ctgaccaaaa agatctcttc gcgcgcatct gtggattttt cgatcatatc
     2461 gattacaaca ttgtgcaggc gaaaattcac acgacacgcc atgggtatgc tcttgatact
     2521 ttcctggtcc tcgactctct tgagctggtc ctcgacccgc tcgacttggc ggagcgccac
     2581 cgcgacatgt cgggctacat acagcatgaa ctcgcccggc aactggagca acgcgaacca
     2641 ttgcaaccac cattgcgtgg ccgcctgagc aggcaactca agcattttcc gatgaagccg
     2701 gaagttgaca tcgaactgga tgaaaaaggc atgttctaca tcctttcgat tgttgctggt
     2761 gaccaagcgg gacttctatc ccgcgtcgca caagtactcg tgcaatttgg cgtggatgta
     2821 cacagcgcgc ggataaacac gcttggggat cgcgccgaag acacttttct gattaccggc
     2881 cagcctctca gtgatgcaaa aacgctgatc cgtctggaaa caaagcttgt gaaggcgcta
     2941 gagacttcgg acccatcaaa cggaaaacct gccgctctcc ttattcgaga agaggttgca
     3001 tagctccgtt atgtaagtct gcgatcgacg gcaggggaag cccggtcaat ccgatcagca
     3061 agcgtcccca tacgaacaat catactcacg atttttcctg ctttccctgt tctactcctc
     3121 gtctcagaca gattgctcgg ccagttcgcg ggcttggagg cgagcgcgca tcttgcccgc
     3181 cgctgcaacc attcttttca aggcggtact cgtttcctcc caggtgcgag tcttaaggcc
     3241 gcaatcagga ttcacccata acttttccgg atcgatcacc agacaagcct tttcgagcaa
     3301 ctcgaccatc tcgacagttt ccggaatcct tggcgaatgg atgtcgtaga ccccaggccc
     3361 gatctcgttg ggatacttga actccgcaaa ccccttcaat aactccatac gcgagcgaga
     3421 ggtttcgatt gtaatcacgt ccgcatccat ctcggcaatg gctggcaaaa tatcgttaaa
     3481 ctccgagtag cacatgtggg tgtgtatctg agtatcgttt ttgacacccg agctggctac
     3541 gcggaaggcc ttgactgccc agtccagata ggcctcccag cttgcttttt ttaacggcag
     3601 tccttcgcga aaagcgggct catcgatctg gatcatacta atgccggcct gctccaggtc
     3661 gaacacctca tcccgaatgg cgagcgcgag ctgcaaggcg gtgactggcc gcggctgatc
     3721 gtcgcgcaca aacgaccaca tcagcatggt gacggggccc gtcaacattc ctttgacggg
     3781 cttttcagtc tggctctgcg catatcgtat ccagtccacc gtcatgggtt cggggcgata
     3841 aatgtctcca tagagaatgg gcggcttgac gcagcgcgaa ccatagcttt gtacccaccc
     3901 gttttcggta aaggcgtagc cccataattg ctcgccaaaa tactccacca tatcgttacg
     3961 ctcagcttcc ccatgcacaa acacgtcaag accaatctcc tcctgccttt taatcgccaa
     4021 cactatctgg ttgcgtatcg actccagata ctgcaagttg ctggcctcgc cttttttgag
     4081 tgctgcacga accctgcgga tttctggagt ttggggaaat gagcctatgg tcgtggtagg
     4141 taaaagggga agctggaagc gtttccgttg caacaaccgg cgctccggaa aggggctggc
     4201 gcgttgacta tcccgccagg tcaggtgctg aagcctttcc tgcacgaccg gactatgtat
     4261 tcgaggggat cgcgcccggg cctgtcttgc acgcgtagcg gcatccagct gctcgcgtat
     4321 cgtctcttca ccctggttca atcctcggcc aagaatcgat atctcatcca gcttctgaac
     4381 cgagaacgca agccagtcct tgatctccgg ttcaagccgc atctcaagct caaggtcaac
     4441 cggagagtgc agcagggaac aacttggcgc aagccataac cgctcaccca gaatcgattg
     4501 agcctctgac aatatcgaaa acgcctgtgc aaggtctgct cgccatacgt tgcgaccatc
     4561 aatgatcccc agcgacagga ttttatccac ggggtagtcc ttgatgaagg cagcaagctg
     4621 cccgggagca cggctcaagt cgatgtgaag gccctctacc ggaagatttt tcagccgccc
     4681 ggcgtggtta tccaccgctt caaaataagt cgtcaacagg agcctgggtc gcgattcgat
     4741 tgattggtgt aaatatacgt acgcctgctc cagggcgcga agccactctt cactcaagtc
     4801 gagtcctagc gagggttcgt caatctgcac ccactcgacg ccggctgtag cgagccgagc
     4861 cagaatatct cgataaaccg gtagcagacg cggtaacaga ttgagacgac tgaagcccgg
     4921 tccgacttct ttgcccagat gaagatacgt gacgggtccg atcagcacga ctttcggacg
     4981 cgctccaagg gcctgaactt cggcaacctc atcgaaaaga cgggacgaag caatgcgaaa
     5041 cactgtgtcg gcactgaatt cagggacaat gtagtggtag ttcgtgtcgt accacttggt
     5101 catctccatc gcgggctgat gcgcggttcc acgcgccatc gcaaagtaca aatccaaccc
     5161 gatgtcgcaa tctcgtggag ggccgaagcg gggaggcaca gcacccagta gtgcagtcat
     5221 gttcagcacg tgatcataga gcgcgaaatc tccgacagga acgaggtcaa cgcccgcacc
     5281 gtgctggatt gaccagtgtt tcttccttag ttccgctgcc gccgcttgca ctcggtcttc
     5341 actaatttcc ccgcgccagt aagcctcaag cgcgtgcttg agttctcgtc gtgcgccaat
     5401 acggggaaat cccaggttat gcgtcagtgc catgaaagtc tccttgttgc ccggttaggc
     5461 cattgcatca ggagacgatt gtgtggcaga ctttcttatg aatcaaatga aatattatta
     5521 aaaatgtatg aaaaaaacta atatcttacc tgataatttt attgctcata gttctactta
     5581 tgtcgctcgc atctatcctt gaattgcgcc acctgcgttc gctcatggcc ttgcatgaaa
     5641 cgggcagcgt gtcccgggcc gcgagacgcg tccacctcac ccagtcggcg ttatcacatc
     5701 agatcaaggc gctgcaggac cactatggat tgcctgtcgt acaacgcaag gggcaatccg
     5761 tcgaactcac cgatgccggc aagcagctcg ttacgcttgc cgaaaaagtg ctgggagaga
     5821 tacaggcaac cgagcgagac atcgcaaaac tgtcccaaaa tgtctccggc agcctgagga
     5881 ttgcactcga atgccatacc tgttttgact ggctgatgcc acttatggac gagttccgcc
     5941 aacactggcc cgacgtggag cttgacctgg tatcaggttt tcacagcgat cctgtcaagt
     6001 tgctggagga aggcaaagcg gatgtggtga tcggctccga aagcaaaccg tgccgcggcg
     6061 tcgtccacga gccgttattc cgttttgaaa ttctcgcggt attggcaact gaacaccccc
     6121 tctccgccaa aagaatattg caccctacag atttcgcgag tgaaacgctt atcacatacc
     6181 ccgttcccga agatcgtatc gatctgattc tccaggtgct gagaccagca ggaattcatc
     6241 cccagcggcg cacggcggaa ctcaccgttg ccattttgca gttggtagcg agtcgccggg
     6301 gagtggcggc attgccgagc tggggaatca agaattacct cgatcatgat tatgttatag
     6361 cacgccgcat cggcgcccag ggtttatgga gcgacctgta tgcatccacg cttaaggaaa
     6421 cggcatcacg ggcgtatatg cgcgattttt tgacgactgc aagaaatacg tgctttgcaa
     6481 cgctggaggg aatcattcca ctggaatgat cagtaaaaaa ccgggaaggg cgggctgaac
     6541 gaaaacgaga acgaacggaa aacaggggag gaaggaaaaa gagtagaaaa gaaggagtag
     6601 ctgaaaggtt ggttgaagcg cagaggttaa tatgatgagt tcatgatgag gggtgagcaa
     6661 acagactgcc tgctcatccc tcatttgcaa acggtcagtg caatgtcact gcggcttcct
     6721 cttcttcctc gcaggcaaga ttggcgcagt acttctcgta aattccatcc agaaaacccc
     6781 ataaggcatc acacgctcgg gcagtcgcgg ccacaacctc agcctgtttc tccggcgtgt
     6841 cggcgaaacg ctcgatcaac gcccattcgg cttgcgagtg atatacatcg gcttcctggt
     6901 gcaccgaaaa gaactcatag ctatccggat tactcatccc atagaagcgg gcaagaccgt
     6961 caattttgac agctgcgatt gaaggtacct gggattcaaa cgcatgtagc gccgcaaggc
     7021 cggcatagaa cggctcattc agacagatat cgcggaatgt cgataccaat cgcgctgttg
     7081 ttggcaaggc cgtggcattc gccagactgc tgtcactgga acccaaagcc agcgcgaacg
     7141 acttccatag ggcaggatgg tttttttcgc cgtgctcttc gctgatcaga tttttcagta
     7201 cttcctggcg aacactgata tcgccgctat tgcattcact atggaagtgc ggtgtgttga
     7261 aatgcacggc actcagataa gtgggttctg cgagaacatt atggaaatac tgttcggcat
     7321 aatggcgcag ctgttccttg gagagcttgc cttctgtcca ggcaacgtag aaaggatgct
     7381 tgagcagatg cctcgcatca atgatggaat cgatcttttg cttaagagat acattagttt
     7441 tcatggtttt cctcttgaac agagatagtg gacggtgggc gtatcatcgc gaaataatgc
     7501 cttcaagtca aatttgtaaa aagaactccg aacgcaaggc ttgcatccgg ctaaccagag
     7561 tccgaacgga ggttggttta tctcttgggt agaacctgtt gtcccttcaa tcgcctataa
     7621 tcctccgcct cgccggacta acgaccagcc ggcttaaacc tataaatcat ggatgagcaa
     7681 tccagcttgt tcgccctgtt taccagcagt tttctcgccg ccacgctatt gccgggcggg
     7741 tccgaagccg ttctgttcgg ggtcctgacc gcctaccccc agctatattg gccggctctc
     7801 ggagttgcaa cgcttggtaa caccctcgga gggatgagct cttatgcgat cgggcgactg
     7861 ctgcctgatg aaaaagcact tgaaaaaaaa ctagggggca atcttcgggg gatcgaatgg
     7921 gtaagcaagc acggcagccc tgttttgctg ctctcgtggg tgcccctgat cggtgatctg
     7981 ttatgcatcg ccgcaggctg gctccgcatc aactgggtgt tggcaacact gttcatggcc
     8041 ctcggcaagt tcgggcgcta ttgggcgatt ggggctgcaa tgaacatctg agatgaggct
     8101 gaaatcccct acgagccagc gaaccggatg ccctgcccac tccaaaaaaa cggcggtttt
     8161 ctaccgccgc ttcctgtcag ccagatcgtt cttccgcaac cataactctt gaccaactgg
     8221 acatagccca gcctcgtcgg ggcgggctaa ccggaagatt caagtaatcc ggtgcgcgag
     8281 aatccgccta tgacggtgcc ttgtccttct gctcctggat cagtttctgc agttcgccgc
     8341 tttgatacat ctcggccacg atgtccgaac caccaacaaa ttccccatta atgtacacct
     8401 gggggatagt tggccagtga gagtattcct tgattccctg ccggaactct ggttctgcga
     8461 gcacatctac ggcaaacagg ttatttacac cacatgcgtt gagcatcttt accgcatggg
     8521 cggaaaaacc gcactggggt gcctggggcg tgcccttcat ataaattacg acgggatgag
     8581 tggtaatttg ctctttaatg acttcttgtg tatccatgtc cggctcgcct ccttttgaaa
     8641 ttttgtaaga ccggcgagtc agcataggcg aaacgagcgt tgttttcaag tgccattaac
     8701 gcaaacgaat tgcgccgaat taaatgtagc cgaaaggtat tctgagaaat aacgttgcgc
     8761 catattgaag gttaccgaaa aggagacctt gatatggtaa aaagtactcg gcgggaatac
     8821 cttgaaacga ttcggaaacg ttatcgcagg gcgtgcaaga aggataagaa gccgatcctg
     8881 gatgagtttt gcgtcaattg cggctatcac cgcaaatatg ccatttagct gctgggccat
     8941 cgcgccagcc tgcacattaa aaagccaggc gccaagccaa tttatgactc aactgaactg
     9001 cttatagtgc tcagcgcatc tggtttgtca gcgcctgctt gaatccgatc acatcggttc
     9061 agaaacaaaa gaccgccctg atgaatacct tcctcaatac ccatccgttt gtcctgaaga
     9121 aaaccattca ggcaaaactc aaaatcatct tcaatgccct aaaatgaatt cccctccctt
     9181 atggcacaac gctatcttct ctatttcggt aacctttttc gatggcgcaa cgcgcaaata
     9241 aaaaaagaca aagcttgcag ctattcgttc tccaaggaaa agtggtatgc aactcctccg
     9301 ctcacgcgca ttattccggc aaggtcagga tgggaaaata catcatggaa gccggcctcg
     9361 ttcaataact ggcaacacct gaacccctga tcgtatccat gctcaaccat tatcattcct
     9421 ccaggcatca ggtaggcagg cgcggacgcg ataatcgagc ggaggcaacg ggtaccatca
     9481 ccaccatcgg tgagggcgat ggatggctca aagcgcaggt ctccctgggt caaatgagaa
     9541 tctccatttg ctacataggg agggttcgat acaatgagat cgaatctttc ccccgccagc
     9601 ccattgaacc aatcgacctg catgaaccgt acgttatcca cgttgagctg catggcattc
     9661 gaacgcgcga caatcagtgc ttcaggcgag atatctaccg cgaccactcg agccagagga
     9721 cgctgtttcg ctatcgcgag cgcgatggct ccactgccgg ttccaagatc aagaatggta
     9781 atggctcggt ttacaggaac acgctccagc gccaaatcca ccagtagctc cgtttccgga
     9841 cggggaataa gcacggcagg ggtaacctcg aaatccaggc tgtaaaattc ccgctctccc
     9901 gtaagataag caacaggttc accttgccga cggcgtccca gaagttccgc aaactgctgg
     9961 gcttgctcga ccgagagctc ttgttccgag tgcgcaatca tatgcgcaag attcagcttg
    10021 agcacatgct gcaacaacat ccgggcatcg gtattgtcga taatcccttg cgcctggcac
    10081 agcgcttcat ggacagtgat cagggcacct cccgattatc ctgatcacgc ttcgagaaaa
    10141 tttttcaggc gcaattgagc ggagatagtg aaaaaaaggg cgatatttga acatggaaat
    10201 ccgaaaaccg gatatgagga atattctctg gtccttattc tcccatcgcg gccaattgct
    10261 ccgcctgatg ttcggacatg agcactgagc acaattcatt caaatcgcca tccatgatct
    10321 gctcgatttt gtagagcgtg agattgatgc ggtgatccgt tacgcggccc tgaggaaaat
    10381 tgtaggtacg aatgcgaccc gagcggtcgc ccgtgcctac gagtgatttt cgggtggcag
    10441 cctgcttgct ctgttgctcc tgcatttgcc tgtcacgaat acgtgcagca agaatactca
    10501 tggcctgtgc cttgttttta tgctgcgatc gcccatcctg acactccacc accgtattgg
    10561 taggcaggtg ggtgatgcgc accgcggaat cggtcttgtt gatatgctgg ccacccgcgc
    10621 ctgaggcgcg gaatgtatca atccgtaatt cagcaggatt caggacgacg tccataatct
    10681 catccgctt
//
LOCUS       DASSHJ010000068        13323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_718334, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000068 DASSHJ010000000
VERSION     DASSHJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 13323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13323
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718334"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFI43_02700"
     CDS             <1..269
                     /locus_tag="VFI43_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET7061068.1"
                     /translation="ELVYQYLTGPRFRQRVEAIVEAFSTMRDDLEKERKVITKQWAKR
                     EEQIMRVMGATVGMYGDLQGIAGKSLQEIEGLELKSLGLYEEDL"
     gene            complement(381..812)
                     /locus_tag="VFI43_02705"
     CDS             complement(381..812)
                     /locus_tag="VFI43_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HET7061069.1"
                     /translation="MSQLEFIAPDPNLCTEEEISKLVHTFYARARRDPSLGPIFESHV
                     ADWDAHFVQMINFWSMKLRGTSRFRGMPMPKHVALPGLSAPLFERWLELFRQTTSEMG
                     NPILQFNADMLAHHIGTRLWQRYQMERYPDREMVELFAAES"
     gene            complement(1109..2071)
                     /gene="rpoS"
                     /locus_tag="VFI43_02710"
     CDS             complement(1109..2071)
                     /gene="rpoS"
                     /locus_tag="VFI43_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HET7061070.1"
                     /translation="MNQLDYSSDEEPFEQPDPLEPEDDVPLEAVEAAPDGAAEIHTDY
                     LLTDVTQIYFNEIGQNSLLTAKEEAELTRLVKQNDFQARQRMIECNLRLVVNIAKHYT
                     NHGVALLDLIEEGNMGMMHALEKFDPERGFRFSTYATWWIRQNIERCIMNQSRTIRLP
                     VHVIKDLNIILQAARHLEAHTGKEPKADDVAHLLGRPVKEVQRMLSLNMSMMSLDAPL
                     DVDPLLSMGDIMPDDNTPGPDLMLEQSEIKDRVQEWLHRLNEKQRYIIEKRYGLNGYE
                     IQTLEQLAATLDLTRERVRQIQLEALQTLRMILRCDGVSREGVF"
     gene            complement(2068..3099)
                     /locus_tag="VFI43_02715"
     CDS             complement(2068..3099)
                     /locus_tag="VFI43_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7061071.1"
                     /translation="MSYRLLAFYTIRKGRFDFAPLQAIFVSLMLAACASTQQNTAPIV
                     DRSTPLPEGAVENLPAGSVPGVPLQQRKTYMVQKGDTLYAIALNHGVSQKDLAEWNNI
                     QDPASLHIGQQLYLSPPSGQAQPTLFGISEPGPQLKVVTTPGAIPQPEGKPGVNTDKL
                     KSEPRAFKVPYSEQALAQFRGMGSAPPTVIPVIAKVNVAPEKSPEALAAAQPQAEAIR
                     TDDGVGWIWPTKGKLLDGFSEATKGIDIAGKPGQAVTAAAGGKVVYSGAGLRGYGKLI
                     IIKHNNIYLSAYAHNDKILVKEGQTVTKGQKIAEMGSTDSDLVKLHFEIRKNGKPVDP
                     LKHLPGAPG"
     gene            complement(3181..3843)
                     /locus_tag="VFI43_02720"
     CDS             complement(3181..3843)
                     /locus_tag="VFI43_02720"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET7061072.1"
                     /translation="MHSRHSGIGMTSQRTRARMIERLRAQGITDEAVLASMGTIPRHI
                     FVEEVLSTRAYDDVALPINYGQTISSPFTVARMSELVRAGANPQKVLEIGSGCGYQTA
                     VLARFAREVYSVERVGPLLTRARARLRELGLGNIRLRHGDGLLGWPDCAPFDAIIVTA
                     AAAQVPRLLLEQLSVGGRMVLPKGDLKQCLCVIEHNSQGFNEIILEQVKFVPIVRGTW
                     KR"
     gene            complement(3876..4616)
                     /gene="surE"
                     /locus_tag="VFI43_02725"
     CDS             complement(3876..4616)
                     /gene="surE"
                     /locus_tag="VFI43_02725"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET7061073.1"
                     /translation="MRILLSNDDGYFAPGLICLAEALSTIAEIIVVAPERDRSGASNS
                     LTLDRPLSLRKSHNGFYYVNGTPTDCVHLAVTGMLDTLPDMVVSGINDGANMGDDTLY
                     SGTVAAATEGFLLGIPSLAVSLAGFSKGNFATAARVAKEIIQRFGNDRLHGPVLLNVN
                     VPDIAHGELQGLEVTRLGRRHKAEPVVKSKSPRGETVYWVGAAGPAQDAGEGTDFFAI
                     KHNRVSVTPLQIDLTRYGQLDAVREWIA"
     gene            complement(4725..4801)
                     /locus_tag="VFI43_02730"
     tRNA            complement(4725..4801)
                     /locus_tag="VFI43_02730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4765..4767),aa:Pro,seq:ggg)
     gene            complement(4939..5319)
                     /locus_tag="VFI43_02735"
     CDS             complement(4939..5319)
                     /locus_tag="VFI43_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7061074.1"
                     /translation="MESTTASNATLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFT
                     QLKPVKRRGNRRYYQHHEVLLVRRIRDLLYEQGFTIHGARSRLDQGGVAVSPAAVSIN
                     PQSKKMDLALLRSEIENVVSLLRS"
     gene            complement(5300..5605)
                     /locus_tag="VFI43_02740"
     CDS             complement(5300..5605)
                     /locus_tag="VFI43_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HET7061075.1"
                     /translation="MTLTKAELADLLFEKVGFNKREAKDMVESFYEEVRVALQNGDGV
                     KLSGFGNFQLREKPQRPGRNPKTGEEIPITARRVVTFHASQKLKAMVEKNQHGKHNS"
     gene            complement(5808..8183)
                     /gene="pheT"
                     /locus_tag="VFI43_02745"
     CDS             complement(5808..8183)
                     /gene="pheT"
                     /locus_tag="VFI43_02745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7061076.1"
                     /translation="MKFSENWLRTFVNPPVPANELADALTMAGLEVEAVEAVAAAFEG
                     VVVAKILAVQKHADANHLAICRVDAGTGAEPLQIVCGAANVRAGIKVPCALPGARLGG
                     NTIRHTRIRGAESGGMLCSAKELGLQEEVEGLLVLPQDAPEGADFREYYELDDTIFTL
                     KLTPNRGDCLGMTGIAREVAAITGETAHPIRIRTVTTESTNELAVCIEAPDACPLYYG
                     RVIRDISMNVSAPKWMVRRLERSGLRSINAVVDVANYVMLEMGQPLHAFDLGRISGGL
                     SGTINVRYAKAGEKIQLLNGENLMLQPDMLVISDEAQPLALAGIMGGSDSGVTNDTTD
                     IFLESAFFSPPAIAGKSFTLGFSSDSAHRFERGVDFGATRDAMERASELILDICGGKA
                     GPVTEAWGGALPRRDPVWLRPQRTRRVLGIDLDEAAMAEILQRLQFAFEYADESFRVS
                     PPTYRFDIAIEEDLIEELARVHGYNRIPSTLPVGELGMLSDPETRRTPSQLRQVMVDR
                     DYQEVINYGFVETSWELELSGNRAPVELKNPLSSQMEVMRSSLLGGLISNLKFNLNHK
                     QARVRLFEIGRCFWKDNEGYLQREMLGGLCYGDTAGEQWGIPARPVDFYDVKGDLEVL
                     CWPVPIDAEPAPHPALHPGKSARISVGGRIAGYLGELHPRLQRKFDLPRPPILFELEL
                     DALIIQALPRAEEISKYPSIRRDIAIVVDEDIDFEVMLASMKSGSSPIISDISLFDLY
                     RGKGVGEGKKSLAFRILLQDTEKTLTDAEADMEVAKLLGILEERFNAALRN"
     gene            complement(8307..9332)
                     /gene="pheS"
                     /locus_tag="VFI43_02750"
     CDS             complement(8307..9332)
                     /gene="pheS"
                     /locus_tag="VFI43_02750"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7061077.1"
                     /translation="MNDLDHLLREAIDLFEGIDDLAELEQAKGQYLGRHGRLTTLLKG
                     LGSVPPEERPSMGMRINRVKEALEEALNSRREAIRQKELEAQLAEERLDVTLPGRGGH
                     SGGLHPVTRVLERICTLFHSIGFDVASGPEIETDFYNFTALNIPENHPARAMHDTFYI
                     GNEAGNYLLRTHTSPVQIRHMQNTQPPLKIIAPGRVYRRDSDLTHTPMFHQVEGLWVA
                     ESATFSGLKGVLVDFMQRFFERDDLRVRFRASFFPFTEPSAEMDIGCVMCQEGCRVCS
                     YSGWLEVLGCGMVHPNVFKHVGIDSEKYTGFAFGMGVERLAMLRYGVSDLRLFFENDL
                     RFLRQFN"
     gene            complement(9429..9788)
                     /gene="rplT"
                     /locus_tag="VFI43_02755"
     CDS             complement(9429..9788)
                     /gene="rplT"
                     /locus_tag="VFI43_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7061078.1"
                     /translation="MPRVKRGVTAHARHKKVLDLAKGYRGRRKNVYRIAKEAVMKAGQ
                     YAYRDRRQKKRDFRALWIARINAAARECGLSYSIFMNGLKKAAIEVDRKVLADLAVFD
                     KQAFAKIAEQAKASLAA"
     gene            complement(9799..9996)
                     /gene="rpmI"
                     /locus_tag="VFI43_02760"
     CDS             complement(9799..9996)
                     /gene="rpmI"
                     /locus_tag="VFI43_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7061079.1"
                     /translation="MPKMKTKSGAAKRFKVRAGGSVKRSQAFKRHILTKKSTKTKRQL
                     RGTVSVNCADVASVRAMMPYA"
     gene            complement(10180..10809)
                     /gene="infC"
                     /locus_tag="VFI43_02765"
     CDS             complement(10180..10809)
                     /gene="infC"
                     /locus_tag="VFI43_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7061080.1"
                     /translation="MWGITIVQEKAARINQEINAPEVRLIGTEGEQIGVVTLAVANRM
                     AEEAEVDLVEIAPTAQPPVCRLMDYGKFRYQESKKKHEAKLKQKQIQIKEVKFRPNTD
                     EGDYAIKLRNLIGFLEEGDKAKITLRFRGREMAHQEFGIRLLERIKADLEPYAIVEQF
                     PRMEGRQMVMVLSPRKKEVKIGKPKEAKEPKQNLHNETGGSPDSGTAHL"
     gene            complement(10814..12724)
                     /gene="thrS"
                     /locus_tag="VFI43_02770"
     CDS             complement(10814..12724)
                     /gene="thrS"
                     /locus_tag="VFI43_02770"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7061081.1"
                     /translation="MAVIRLPDGSERRYENPVTVGQVAASIGSGLGRAALAGKVNGKL
                     VDVSAPIESDSDLKIITDKDAEGLEVVRHSTAHLLAHAVKELFPDAQVTIGPVIEDGF
                     YYDFSYKRPFTPEDLAAIEKRMVEISKRDLKVERQVWDRSEAINFFKNQGEHYKAQII
                     ESIPGDEDVSLYAQGNFTDLCRGPHVPSTSRLKVFKLMKLAGAYWRGDSHNEMLQRIY
                     GTAWIRKEDQDAYLHRLEEAEKRDHRRLGKQLALFHTQEEAPGMVFWHPKGWVIWQQV
                     EQYMREIFRDNGYLEIRTPSVLDKSLWERSGHWENFRENMFITRAEDRDFAVKPMNCP
                     GHVQVFRQGLKSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRGFTQDDAHIFCTESQV
                     QTEAIQFIDLLQKVYSDFGFDEILVKLSTRPIKRVGADAQWDRAEAALQSALEHKKLT
                     WELQPGEGAFYGPKIEFSLKDSIGRVWQCGTLQLDFSMPERLGAEFVAEDNSRQVPVM
                     LHRAILGSMERFIGILIENYAGALPLWLSPDHAVVLNISEGQADYAQKVAEELRRNGI
                     RAQADLRNEKITYKIREHSLQKLPYQIIVGDKEVAAEMVSVRNRAGANLGQVPLTALI
                     ERLKGEIAAKTH"
     gene            complement(12931..>13323)
                     /locus_tag="VFI43_02775"
     CDS             complement(12931..>13323)
                     /locus_tag="VFI43_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HET7061082.1"
                     /translation="LEAGATASYGTVVEPCNHVQKFPFPGIAMFHYALGASALEAYWK
                     SVAWPGEGVFIGEPLARPFAPRIRKAGPGQFELHVFSPRAGRLRFERSISAAGPFKPL
                     LRQVMLGRGENVIRFNFSESEGYLRLRW"
BASE COUNT         2907 a         3800 c         3417 g         3199 t
ORIGIN      
        1 ccgaactggt ttaccaatac ctcacgggtc cccgcttccg tcaacgcgtg gaggccatcg
       61 tcgaagcatt ttcgacaatg cgggacgacc tggaaaagga acgcaaggtt atcacgaagc
      121 aatgggctaa acgggaagag cagatcatgc gcgtgatggg cgccacagtg ggaatgtatg
      181 gcgacctgca gggaatcgct ggcaaatcgc tgcaggagat cgagggactc gagctgaaaa
      241 gcctggggct ctacgaagag gatttataac aacaggaacc tggaccagcc cgaggcccgg
      301 gaggagcgag ccgaaaggtg ttacaaagga acgtcttgtt atagacgcac ggccggctcc
      361 atcctcttct ttcgatcaga ctagctttcc gcggcgaaga gctcgaccat ctctctgtcg
      421 ggatagcgct ccatctggta gcgctgccac aatctcgttc cgatatgatg cgcaagcata
      481 tccgcattga attgcaggat cgggttcccc atttcgctgg tcgtctgacg aaacagttcc
      541 agccatcttt cgaacagtgg cgcgctgagt ccaggcaaag ctacatgctt gggcatgggc
      601 atgcccctga atctgctcgt tccgcggagt ttcattgacc agaaattgat catctggaca
      661 aagtgggcgt cccagtccgc gacatgcgac tcgaatatgg ggcctaaaga aggatcgcgt
      721 ctggctcttg cataaaacgt gtgaaccagc ttggaaattt cttcttctgt gcatagattg
      781 ggatcaggag caataaactc tagctgtgac atggctataa attaaataag gagatcagat
      841 atcgaaatgg atgctcggga atttaaaaag cgaggggaga gcgctcattc tcgcttctcg
      901 caacgccttt tatcacaagt ttatgtcaaa aggcggagca agtaaagacc ttgctgcctt
      961 cggctttctc gtatcgggga gttgcggttg agttgtcagg aaagcagtat acttcacctg
     1021 cccgggaagg gagtatggat gatgaaaaag cgggaaaaag gccgcaagcg gcgagccagt
     1081 agcccgaata acgccttatt gaaactcgct aaaacacccc ctcccgcgat acgccatcac
     1141 accgcaatat catgcgtagc gtttgcagcg cttccagttg aatctggcgt acgcgctcac
     1201 gcgtaaggtc gagtgttgcg gcaagttgtt ccagggtctg gatttcgtaa ccattgagac
     1261 cgtagcgctt ctcgataatg tagcgttgct tctcattgag ccggtggagc cactcctgga
     1321 cccggtcctt tatttcagat tgctcgagca tgagatcggg accgggcgtg ttgtcatcgg
     1381 gcatgatatc ccccatcgat aacagcggat caacgtccag cggagcgtca agtgacatca
     1441 tgctcatgtt caaggacaac attcgctgga cctccttgac cgggcgcccg aggagatggg
     1501 caacgtcgtc agccttgggt tccttccccg tatgggcttc gaggtggcgc gcggcctgca
     1561 ggataatgtt caaatccttg atgacgtgaa cgggcaaacg aatcgtgcgt gactgattca
     1621 tgatacagcg ttcgatgttc tggcgtatcc accaggtcgc gtaagtcgag aacctgaagc
     1681 cgcgctccgg atcgaatttt tcgagggcgt gcatcatgcc catattgcct tcttcgatca
     1741 aatccagtag cgctaccccg tgattcgtat agtgctttgc aatattcacc accaaccgga
     1801 ggttgcactc gatcatcctc tggcgggcct gaaaatcatt ctgcttgacc aaccgcgtca
     1861 actccgcttc ttctttcgcc gttagcagcg agttttgtcc gatctcgtta aaatagattt
     1921 gcgtgacatc ggttagcaga tagtcggtgt gaatctctgc cgcgccatcg ggtgctgcct
     1981 cgacagcctc gagcggtacg tcatcttccg gttccaatgg atcgggttgt tcaaaaggct
     2041 cttcgtcgct gctgtaatcg agttggttca tcccggcgct cccggtaagt gtttaagtgg
     2101 atccacaggc ttaccattct tgcggatttc gaagtgaagc tttaccagat cgctatcggt
     2161 gcttcccatt tcggcaatct tttggccttt ggttactgtt tgcccttctt ttacaaggat
     2221 tttgtcgtta tgtgcatatg cgcttaggta aatattgttg tgcttgataa tgatgagttt
     2281 tccgtatcct cgaaggccgg ccccgctgta aaccactttt ccccctgcag cagccgtgac
     2341 cgcctgtccg ggcttgcccg caatatcgat tccttttgtg gcttccgaaa acccatcaag
     2401 caattttcct ttcgttggcc agatccatcc gactccatca tcagttctaa tggcctcagc
     2461 ctggggttgc gcagcagcca gggcttcagg gctcttttcg ggtgcaacat tcaccttcgc
     2521 gataacgggg atcaccgttg gaggagcact ccccatcccc ctgaactgag cgagcgcctg
     2581 ctccgaataa ggcaccttga atgccctggg ttcacttttc agtttgtcgg tattcacccc
     2641 cggcttccct tccggttgtg gaattgcacc aggagtggta acgactttta attgcggacc
     2701 aggttcagag ataccgaaaa gggtgggttg cgcctgccct gagggtggcg aaagatagag
     2761 ttgctgcccg atgtgaagcg aagcggggtc ttgtatattg ttccactccg ccaggtcttt
     2821 ctggctgacg ccatggttaa gtgcgatcgc gtaaagcgtg tcgccctttt gtaccatgta
     2881 agtcttccgc tgttgtaagg gcactccggg gactgaaccg gccgggaggt tctcgacggc
     2941 cccttcggga agcggtgtag agcgatcaac tatcggagca gtgttctgtt gcgtcgaggc
     3001 acatgcagcc agcatcagtg acacgaaaat ggcttgcaaa ggggcaaaat caaaacgtcc
     3061 ttttcttatt gtataaaacg cgagaagcct atagctcatg acactcttag ataataattt
     3121 tagtaaacct ttgcttgaat ccccgcagtt gtgcactcct tccttctcac attcttgtct
     3181 tcaacgcttc catgtgcccc gcacgatcgg cacgaacttg acctgttcga gaattatctc
     3241 gttgaatcct tgagaattat gttcgataac gcacaaacac tgcttcaaat cacctttagg
     3301 taaaaccatc ctgcccccaa cggagagttg ttcgagcaac aagcgcggaa cttgtgcggc
     3361 ggcggcggtc acaatgattg cgtcgaaagg ggcgcagtcc ggccaaccga gcaagccatc
     3421 cccgtgtctg agccgaatat tgccaaggcc cagttcgcga agacgcgcgc gagcgcgggt
     3481 cagtaagggg ccaactcgtt cgacggagta cacctcgcgc gcgaaccggg ccagcacggc
     3541 tgtctgatat ccacagcccg aaccgatttc gagcactttt tgcggatttg cgcccgctcg
     3601 cacgagttcg ctcatgcgag cgacagtgaa aggactggaa atggtctggc cgtaattgat
     3661 aggcaacgca acatcgtcat aagcccgggt tgagagaact tcttcgacga aaatgtgccg
     3721 gggaatggtg cccatcgagg ccagcactgc ttcatcagtg atcccttgcg cccgtaagcg
     3781 ctcgatcatg cgcgcacggg tgcgctggga ggtcatccca attcccgaat ggcggctatg
     3841 caagctcggc gtacctttta aagggctcct gaaggttatg cgatccattc tcttacggcg
     3901 tcgagttgtc cgtaacgagt caggtcgatc tggagtggag tgacggagac gcggttgtgt
     3961 ttgatcgcaa aaaagtccgt tccctcacct gcatcctgtg caggcccggc cgccccgacc
     4021 caatatacag tttccccccg gggactcttt gatttgacca ccggttccgc tttgtgccgg
     4081 cgaccgagcc gcgtcacctc aaggccttga agttcgccat gggcaatatc gggaacatta
     4141 acgtttaaaa ggaccgggcc gtgcaaccgg tcattcccga aacgctggat gatctccttt
     4201 gccacgcgcg cagctgttgc aaaattcccc ttggagaaac cggcaagcga aaccgcaaga
     4261 gacggtatac cgagtagaaa gccttctgtc gccgccgcta ccgtaccgga atagagggta
     4321 tcgtcgccca tgttggctcc atcgttgatg ccggatacta ccatgtcagg taatgtatcg
     4381 agcatcccgg taacagcgag atggacgcaa tcggtgggtg ttccgttgac gtaataaaag
     4441 ccgttatggg atttgcgcag gctcagaggc ctgtcgagag tcaaggaatt gcttgcgccg
     4501 ctgcgatcgc gttcgggagc aacgacgatg atttcggcaa tggttgaaag cgcttcggca
     4561 agacagatca ggccaggcgc aaaatagccg tcatcgttgc tcagcaatat gcgcatttga
     4621 acgttacccc aggttttttt gcttatcagg gaaggacaac gggaaacagt acgaacagca
     4681 ctgcttcccg ccgggaatat atagatatta cggatatgct tgactggtcg gggcgagagg
     4741 atttgaacct ccgaccactt gcaccccatg caagtacgct accaggctgc gctacgcccc
     4801 gaaggttgaa attataacaa aaacgggcaa cccaggaatg gttgcaggca cctccgcgac
     4861 gatagagacc aggtggctga cctgaaatcg gttctgaagc tgagaatgat cgtccccgac
     4921 aagcagtaac tcgctctatc aggagcgaag caaggaaaca acgttttcga tctcgctccg
     4981 tagcaacgcg agatccattt ttttggactg gggatttatc gagaccgcag caggcgaaac
     5041 agcaacccct ccctggtcca atcgactgcg tgcgccgtga atagtaaagc cctgctcgta
     5101 gagcaaatcg cggatgcgcc gcacaagcaa gacttcatga tgctgataat agcggcggtt
     5161 accgcggcgc ttgacgggct tgagttgcgt gaactcctgc tcccagtagc gcagcacgtg
     5221 cggcttgacg ccgcacagtt cgctcacttc gccaatagtg aagtaccgtt ttgcaggaat
     5281 gggaggcaac gtcgcgttgc tagctgttgt gctttccatg ctggtttttt tccaccattg
     5341 ccttcagttt ctggctcgcg tgaaaagtaa caactcgccg ggctgtaata ggaatctcct
     5401 cgccggtctt tggattgcga cctgggcgct gcggtttttc gcgcaactga aaatttccaa
     5461 agcccgaaag tttcacgccg tccccgttct gcagcgcaac gcgaacctcc tcatagaagg
     5521 attccaccat gtctttggcc tcacgcttgt taaagccgac cttttcgaac agcaaatccg
     5581 ccagttcagc ctttgttaac gtcatgtctc ttcacccgtt tacccgttgt ctatttttaa
     5641 atcttcctga tgaaaggccc gtagagggcg ttgaggaatc tccaagcaca tcctccgcgc
     5701 ctgttgactt gttcgcagtt ccttgggatc agcaactcgc catttttgga attattaacc
     5761 ggcgcgcgca ggcgcaccca ggtttcttga ttgtcagcct gactttttca attccgcagt
     5821 gcggcattga agcgctcttc caggattccg agcagttttg cgacttccat atctgcttcc
     5881 gcatctgtca gcgttttttc agtatcttgc aataagatac ggaatgcaag actttttttg
     5941 ccttccccaa cccctttgcc gcggtagaga tcgaacaggg aaatgtccga aataatggga
     6001 gagcttccac tcttcatgga ggccagcatg acctcaaaat cgatatcctc gtccacgacg
     6061 atcgcaatgt ctcgccgtat ggatggatat tttgaaatct cctctgctct cggtagagct
     6121 tggataatca gtgcgtcaag ctccagttca aacaggattg gaggtcttgg cagatcaaat
     6181 tttctctgca ggcgcgggtg gagttctccc aggtaacctg caattctccc gcccacgctt
     6241 atccttgctg acttccccgg atgcagcgct gggtgcggtg caggctcggc atcgattggt
     6301 accggccagc aaagtacctc gagatcccct tttacatcgt aaaaatctac tggacgggcg
     6361 gggattcccc attgctctcc ggccgtatcg ccgtagcaga gacctcccag catttcacgc
     6421 tgcaaataac cttcattatc tttccagaaa caacgtccga tttcaaagag gcggaccctc
     6481 gcctgtttat ggttcaggtt gaatttcaga ttggagatca agcctccaag aaggctgctg
     6541 cgcatcactt ccatctggct ggataaaggg tttttcaact ccacgggagc tctattaccc
     6601 gacagttcca gctcccatga agtctccacg aaaccataat tgatgacttc ctgataatcc
     6661 ctgtcaacca tcacctgccg caattgtgag ggggttcgcc tggtttcagg atcgctcaac
     6721 atgccgagtt caccgacggg cagagtggat ggaatgcggt tgtacccgtg cactcgcgcc
     6781 agctcttcga tcagatcctc ttctatggca atatcgaagc gataagtcgg tggcgagacg
     6841 cgaaagcttt catccgcgta ctcgaacgca aattgcagcc gctggagtat ttctgccata
     6901 gccgcctcgt caagatcgat cccgagcacg cgacgcgttc tctgcggccg cagccatact
     6961 ggatcacggc gaggcaaagc tccaccccag gcctccgtga ccggcccagc cttgcctccg
     7021 cagatgtcga gtataagttc gcttgcccgt tccatcgcat cgcgcgtcgc gccaaaatcg
     7081 acgccccgct cgaagcgatg tgcagaatcg gagctgaagc ccagggtgaa ggacttgccg
     7141 gcgattgctg ggggcgaaaa aaaagcgctc tccagaaata tatcggtcgt atcgtttgtc
     7201 acgccactgt cgctcccgcc catgattccc gctaacgcca atggctgggc ttcatccgag
     7261 atgaccagca tatccggctg aagcatcagg ttttcaccat tcaacagctg tattttttcc
     7321 cctgccttcg cataacgcac gtttatagta cccgagagcc cacccgaaat acggccaagg
     7381 tcgaacgcat gaagcggctg ccccatctcc agcatgacgt aattggccac atccacgacc
     7441 gcgttgatgc tgcgcaaccc gctgcgttcc aggcggcgta ccatccactt cggggcggat
     7501 acgttcatgg agatatcgcg aatgactctg ccataataaa gcggacaagc gtctggcgct
     7561 tcgatgcaaa cggccagttc attagtactc tcggtagtga ccgtgcgaat tcgaatggga
     7621 tgagcggttt cgccggtaat agctgcgact tcccgcgcaa tccccgtcat cccaaggcaa
     7681 tcgccacggt tcggcgttag cttgagggta aagatcgtgt cgtccagctc gtaatactcg
     7741 cgaaaatcgg ctccttccgg agcatcctgg ggaagcacaa gcaacccttc aacctcttcc
     7801 tgcaacccga gttctttcgc ggaacataac atgcctccgg actcggcccc acgtatcctc
     7861 gtgtgtctta ttgtattgcc gccgagccgc gcacccggga gtgcgcacgg caccttgatg
     7921 cccgcccgca catttgccgc cccgcagacg atctgcaacg gctcggcacc tgttccggcg
     7981 tctactcgac atatggcaag atggttggca tccgcatgct tttggacggc aagaatcttc
     8041 gcaacgacca ctccctcaaa ggctgccgca acagcctcga ccgcctcgac ctccaggcca
     8101 gccatcgtta gcgcgtcagc cagttcgttc gctggtacgg gcgggtttac gaatgtgcgc
     8161 aaccagtttt ctgaaaattt catcgaattt attttctatg acagggcaat gcaaagaaca
     8221 tccgggattg ttgcagatca tcctgccttt cgtgcaacct ccaggaggcc gacacagcta
     8281 tcctgacctg aactgctgcg ggattgctag ttgaattgcc tcaggaaccg gagatcattc
     8341 tcgaaaaaca atcggaggtc gctcacgccg tatctcagca tcgccagacg ctccacgccc
     8401 attccgaacg caaagccagt atatttttcg ctgtctatac cgacatgctt gaaaacgttc
     8461 gggtgcacca tgccacatcc cagcacttcc agccatccgc tatagctgca gacccggcac
     8521 ccctcctggc acatcacgca cccgatgtcc atttcagccg aaggctcggt aaaaggaaaa
     8581 aaagaggcgc ggaaccgcac ccttaaatcg tcgcgctcga aaaaccgctg catgaaatcc
     8641 accagcaccc ccttcagacc ggaaaacgtt gcgctttcgg caacccacag gccttccacc
     8701 tgatgaaaca tgggggtatg ggttaaatct gaatcacgcc ggtacacccg tcccggtgcg
     8761 atgatcttca agggaggttg agtgttctgc atatgccgaa tctggacggg agacgtatgc
     8821 gtgcgcagca ggtaattgcc tgcctcgttc cctatgtaaa aagtgtcgtg cattgctcgc
     8881 gccgggtgat tttccggaat gttcagcgcc gtgaaattgt aaaaatccgt ctcgatttcc
     8941 ggcccggatg ccacgtcaaa gccgatcgaa tgaaacagcg tacatatccg ctccaaaacg
     9001 cgtgtcacgg gatgcaagcc ccctgaatga ccgccgcgtc caggcaaggt aacatccagc
     9061 ctttcctccg ccagttgcgc ttcaagctct ttttgccgta tggcctctcg gcgcgagttg
     9121 agcgcctctt ccaacgcttc cttcacgcgg ttgatgcgca ttcccatgga aggacgttct
     9181 tccgggggca cgctgcccag ccccttcagc aaggtggtca gcctgccatg ccggcccagg
     9241 tactgcccct tcgcctgctc cagctccgcg agatcgtcaa taccctcaaa cagatcgatt
     9301 gcttcgcgaa ggaggtggtc cagatcattc atgaaaatat agtgggaaca agggctgagc
     9361 ccgtaacaac aaaaaaggag gcaaggcgct tctgccttca gcctcctcaa gtcctattta
     9421 tggggaatct atgctgccag gctggccttt gcctgttccg ctatttttgc aaaggcctgc
     9481 ttgtcaaata ctgcaaggtc ggccaacacc ttgcgatcga cctcgatcgc ggctttttta
     9541 agcccgttca tgaatatgct ataggacagg ccgcactccc gtgccgccgc attgatgcgg
     9601 gcaatccaca gagcgcgaaa gtcgcgtttc ttttgacggc ggtcacggta ggcatattgc
     9661 ccggccttca tgaccgcttc cttggcaatc cggtatacgt tcttccggcg tccgcgatag
     9721 cccttggcca ggtccagcac cttcttgtga cgggcatgtg ccgtcacccc acgttttact
     9781 cttggcattt tcccctcctc acgcgtaagg catcattgcg cggacggatg cgacgtcggc
     9841 gcagttgaca ctgacggttc cgcgcaactg gcgcttggtt ttcgtcgatt tcttcgtgag
     9901 gatgtggcgt ttgaatgcct gcgagcgctt gacactcccg ccggcgcgca ctttgaagcg
     9961 cttcgccgcc ccactcttcg ttttcatctt tggcatgaat gctcctgctt tagcatgacg
    10021 tcgggcgctt cctgcaataa tgacccagga aagcttgatg gcccgaaaat cacttgtttt
    10081 aaaaaagcat accggatggc atgctcgcgt gagaaaaact tctgcaacgt ccgattagtt
    10141 ccctttttga tgggaagagc ttttcgcctc ttgttctgct cagaggtggg ccgtaccgga
    10201 gtccggcgag cctcctgttt cattgtgcaa attttgcttg ggttccttgg cttccttcgg
    10261 cttcccaatt ttcacttctt tcttccttgg ggataggacc atgaccatct gcctgccctc
    10321 cattcgggga aactgctcaa ctattgcata cggctccagg tccgccttta ttcgttccag
    10381 aaggcggatg ccgaattcct ggtgcgccat ttcccgtccc cgaaaacgta aggtgatttt
    10441 ggctttatcg ccctcttcaa gaaaaccgat caagttccgc agcttgatgg cgtaatcacc
    10501 ctcatccgta ttgggccgga acttgacttc ctttatctgg atttgtttct gcttgagctt
    10561 ggcttcgtgc tttttcttgc tttcctgata acgaaatttg ccataatcca tcaaacggca
    10621 aacgggtggc tgagcagtag gtgcaatttc caccaggtcc acttccgctt cttccgccat
    10681 gcggttcgca acggcaaggg tgaccactcc gatctgttct ccctctgtgc caatcagtcg
    10741 cacttccgga gcattgatct cttggttaat tcgtgctgct ttttcctgaa ctatagtaat
    10801 tccccagatt tggttagtgt gttttcgcgg ctatttcgcc cttcaaacgc tcgatcagcg
    10861 ccgtcaaggg cacctgtccc aagttagcgc ccgcgcggtt tcgaaccgaa accatctctg
    10921 cagccacctc cttgtcaccc acgataattt gataaggtaa tttctgcaaa ctatgctccc
    10981 tgattttata ggtaattttt tcatttctca agtctgcctg cgcccttatg ccattgcgcc
    11041 ggagctcctc ggcgactttt tgtgcataat ccgcctgtcc ctccgagata ttgagcacga
    11101 cagcgtggtc aggcgacagc cataagggaa gcgcgccggc gtaattctcg ataagaatgc
    11161 cgataaagcg ctccatcgat cccagaatcg ctcgatgcag cataacgggg acctggcgcg
    11221 aattgtcttc ggccacgaat tccgccccca gccgttccgg catcgagaaa tcgagttgca
    11281 atgtgccgca ctgccacacg cgcccgatgc tgtccttgag cgaaaactcg attttcggtc
    11341 catagaaagc gccttctccg ggttgcaact cccaggtcag cttcttgtgt tccagcgccg
    11401 attgcagcgc agcctccgcc cggtcccatt gcgcgtcggc gcccacgcgt tttattgggc
    11461 gtgtcgacaa cttcaccagg atttcgtcaa agccgaaatc ggaatagact ttttgcagca
    11521 aatcgatgaa ctgaattgcc tctgtttgaa cttgggattc ggtgcaaaag atatgggcat
    11581 catcttgagt gaatccgcgc acccgcatga tgccatgcaa cgctccggag ggctcgtttc
    11641 gatggcatga accgaattcc gccaaccgca agggtaaatc cctgtaactt ttcagtccct
    11701 gcctgaatac ctggacatgt cccgggcagt tcatcggctt tactgcaaag tcacggtctt
    11761 cagcgcgggt gatgaacatg ttctcccgga aattttccca atgcccggag cgttcccaca
    11821 aactcttgtc aagaaccgag ggcgtgcgaa tctccagata accgttatcc cggaatatct
    11881 cccgcatata ctgttccacc tgctgccaga tcacccatcc tttgggatgc cagaacacca
    11941 tgcctggggc ttcttcctgg gtatgaaaca aggcgagctg cttgccgagc cggcggtgat
    12001 cgcgcttttc cgcctcttcc agccgatgga gataggcgtc ctgatcctcc tttctgatcc
    12061 aggcagtgcc gtagatgcgc tgcagcattt cattgtgcga gtccccgcgc cagtaggcgc
    12121 ccgcgagctt catgagcttg aaaaccttga gcctcgatgt actcggaaca tggggccccc
    12181 ggcacaggtc tgtaaaattt ccttgagcat aaagcgacac atcctcgtct ccgggaatgg
    12241 actcgatgat ctgggcctta tagtgttctc cctgattctt gaaaaaattg atcgcttccg
    12301 accgatccca tacctgccgc tccactttta ggtcgcgctt gctgatttcg accatccgtt
    12361 tttctatcgc ggccaggtct tcaggcgtaa agggacgttt ataggagaaa tcgtaataaa
    12421 agccgtcttc aataacgggc ccgatggtaa cctgggcgtc cggaaagagt tccttgactg
    12481 catgcgccaa cagatgggca gtggagtgac ggacaacttc cagcccctcg gcatccttgt
    12541 ccgtaatgat tttcaggtcg ctgtcgctct caatgggagc ggaaacgtcc acgagcttac
    12601 cgttgacttt gcctgcgagt gcggcgcgcc caagcccgct gccgattgaa gccgccacct
    12661 ggcccactgt caccgggttt tcataacgtc gctcagaccc atccggtaag cgaatgacag
    12721 ccacgattct tcctttttta aaaaagtttc aagagatttg taattgcccg gagatcaacc
    12781 aaaagcggga caccgatcaa caactgtgct gataacattt tcacacagat tggcctcaga
    12841 ccaaaatcgg acgacgggtt ttctttcgat gtgctcttca cgttgcatct tccgggaaga
    12901 caagccgtgc ctctcttggc gccgttccct tcaccaacgc aaccttagat aaccctcgga
    12961 ttcagagaaa ttgaagcgta tgacattttc gccccggccc agcatcacct gccgcagcag
    13021 cggcttgaag gggccggctg cggaaatcga tcgttcaaag cggaggcgcc ctgcgcgggg
    13081 agaaaaaaca tgaagctcga actgtcctgg tcctgcctta cgaattctcg gcgcgaacgg
    13141 ccgggcaagg ggctcgccta taaaaactcc ctcccccggc cacgccacgc ttttccagta
    13201 agcttcaagc gcacttgcgc caagcgcgta atgaaacatg gcaattccag ggaagggaaa
    13261 cttctgaacg tgattgcatg gttcaacaac cgtcccatag ctcgctgtag cacccgcttc
    13321 gag
//
LOCUS       DASSHJ010000069        15022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_404664, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000069 DASSHJ010000000
VERSION     DASSHJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 15022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15022
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404664"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..1098
                     /locus_tag="VFI43_02780"
     CDS             391..1098
                     /locus_tag="VFI43_02780"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET7061083.1"
                     /translation="MTKLVLLRHGESISNRDGHFTGWDDVELSEKGEREAVRAGTWLK
                     EAGFKFDACFTSELKRATRTLHIVLGEMGLDCLPIRRSWRLNERHYGALEGIDRLSAI
                     RKFGIFPVLDSQLRFNASPPPLDPSDVRFPGNQSRYSGIDKSELPRSESMGQALLRVL
                     PYWRETIVPELQGGKQVLVVAHKHILRALIMQLDSLSAVQLIKLSIANGRPLVYELDK
                     KLNPIRHYYADQKDGFR"
     gene            1185..3827
                     /locus_tag="VFI43_02785"
     CDS             1185..3827
                     /locus_tag="VFI43_02785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7061084.1"
                     /translation="MTQFSIVIPTLNEAENIDPLLTRLFSPELEKKSFEVIIVDDGST
                     DDTQDKVRAWETRKNVRLVARHGKPDLTASVLEGVALARGEVIVVMDADLSHPPERLL
                     ALTAPILEGTHDVAIGSRYVPGGSTEGWPVHRQWLSRLGGWLARPLCDVNDATSGFFA
                     FRRDLAATIAERARGYKILLELLMASQGKLRVIEVPICFTDRTRGKSKLSFSHQWTYL
                     QRLMTLAGGTVSVGTASRFAAVGLFGVVVDALVFQLLMSNGTRLATAHIVSFLVAASV
                     NYTLNSKWSFRPHHQGRLQWQQFSRYLTVAVFALLMRGGVLALLVYNWHVPSFLSIFP
                     AIAATAVINYLGSAFYVFPAEQDRPSPDVSWRVASFGIIVFSLLLRLIYLDMVELIPD
                     EAYYWNYAQHMDLSFYDHPPMVAWLIWLGTSIFGNSEFGVRFGAFCCGLVTMGYMYEL
                     ARNLYDKTTAIRTVLLLAVLPFFFATGSVMTADAPLVAAWAATLYYMERALLADRRLA
                     WLGMGIAFGLGMLSKYTLALLGLAALVFVIADPSSRRWLRRPHPYLAAMLAFLLFLPV
                     IIWNMRHNWVSLSFQSSRIKGVGDDQFSVHLLLLHLMVLLTPVGLVAAFVALAPNRDR
                     NRSLSADRRQLFIWSFAGVPLAVFVMLSISDSLRFHWTGPLWLALLPTIAWMMGQTSS
                     ASAIVRRLQAVWKPTIAICLFLYAFSMHYLVLGIPGIPYQGIYKGFPEHYFWREATKG
                     VEQIAEEVRQETGQEPIVVGMSKWSVASIMSFYNRGKPMEIRSRNMFGDSGAMYDFWY
                     PSEPPTARPIILVGMWPHNLERTREDRDIGTMLVEPGPVKDRAISQDGKPLRNVYYRV
                     AHGYVGNTKLGESRVADVKGKPDN"
     gene            complement(3897..5162)
                     /locus_tag="VFI43_02790"
     CDS             complement(3897..5162)
                     /locus_tag="VFI43_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7061085.1"
                     /translation="MTHEIARQPVAHDDEPASASELIRSARAWQNALVKFALFFFGIA
                     LWAKWLSLGAGILLAVAWILDNGPRRLDRAIKEPLVLAILILCALLMLGLLWGEYPES
                     GRLKWKRYFAFLVFIPFLSLLNKERLFWAITGLVTGYAMVLLIGVYHRLLGEQGVPLL
                     GMSYLTFSSMLGIGVILSIYAAGEETGKKIKAGCWLLALLLLYLQFNEYGRGALLATL
                     LGSALLVVARYKTEKKVLLAVTGLLAAIIIAFSYSGVLQSRLSQLSADIELARLGKYD
                     TSLGYRLALWDVGVRGIAEKPLFGHGTGAPESYFEKTVKTYKDGVYKDLPQYMETSHY
                     HNDWIEMGMHLGALGVLSFTFLLWSWFQTFKRHGLPVLGAALICFIFISGLADTFALY
                     TKILTFLLAFTAVAISGKEEARGSETIAV"
     gene            5512..6846
                     /locus_tag="VFI43_02795"
     CDS             5512..6846
                     /locus_tag="VFI43_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061086.1"
                     /translation="MKALFFLRHYNDIDHITPVISKWVEAGHSCDVILVGNPKFRSDY
                     RIRFVSSLRNVTVDVIRDVLPSTEFLRWRLQMLLLSNSLRRSFIGPLVSALARFYDAK
                     RREPAWRDSAQRLLERSFKDVEDGVVVFDWITKDSPVSVEWVEHVVATARSRGIGAVS
                     LPHGDSPHANRMIREGEWTLKPDTLFSAAKIFDKLVIPNELCAGRFRPFLENSAIEVL
                     GSPRYCDEWLARLATLSPPAVLPSRPGIRLKIVLFLRKSNFTTFWEEVGEVIGMISTF
                     PDVELIIKPHTRGGWRQPLTRNSSLRHMRNVSVAADNVHSIPLMNWADVIIDLATSVV
                     FEAVKAKKPVLAADYLHCGRSALAYYIPETELKCRDDVYRKIDEFLTIGCESFYVEEH
                     RQRFIEEMLHVGGADVLPRYVALLESQARDKRGTGEVLMLDGRGSIAEQTST"
     gene            6856..7944
                     /locus_tag="VFI43_02800"
     CDS             6856..7944
                     /locus_tag="VFI43_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7061087.1"
                     /translation="MTSGQQRVLQSARSPEIHFGGPAELPGHLRNVLAERIAAVPGGG
                     SIDWVTYYFRDLQLAQALIKAKNRGVRVTVILEGRPRIPHANDSVIAMLSGADGLGEG
                     FRLVALPGIPSPSTKSWKPQLHEKLYCFSHPRPIAFIGSFNPSGNMPDEDPEIIREIG
                     DQDRGYNVLVGFIEPDLVEKLVDHARQIHQVSPSIFYRFSPDANRAIHGSDTAIHFWP
                     RANPHPVIRFLHQVSSKARVRVVASHIRTASAVETMITLARRGVALEIFAEATCRRVT
                     PRVERLLSSAGIRFRRIRISGELPMHLKFVLIEDGNQVNSIFGSFNWTKPSFWLNHEI
                     AAISSNREIFEAFAERWDMLESEKSDKT"
     gene            complement(8070..8906)
                     /locus_tag="VFI43_02805"
     CDS             complement(8070..8906)
                     /locus_tag="VFI43_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7061088.1"
                     /translation="MSQAYKGTPFDLAGRVVLISGASGLLGREFALAAASAGAHLVLG
                     DLDSDKLESLKGEIVSAYPDGEVLAHVLDVVSPESCRSIAEACETRFNRIDAVIHSAA
                     IDPKFEPGSDTSRFSRFTEFPVELWQTSLEVNLTGAFLLAQATCKVMEKSGKGSIIFL
                     GSNYGLVGPDQRIYRKAGQEQQTYKPAVYSVCKSGLLGLTKFLAAYYMNTSIRVNMLT
                     PSGIWNQHDKEFAANYSARTILGRMSEKNEYRGAIIFLASDASSYMTGANLVVDGGWT
                     AL"
     gene            complement(8903..9922)
                     /locus_tag="VFI43_02810"
     CDS             complement(8903..9922)
                     /locus_tag="VFI43_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7061089.1"
                     /translation="MKALVVGLGSIGVRHLNNLHALGVQELAVVRSRNLVPFHAIVPK
                     DITVFHDLDLALSQGYDLVVVANPTSLHLETLVRALEAGSHVYVEKPLADENGPLFEL
                     APYLSTNDSKVLVGCQLRMHPGLRKIEEWVRQDKLGQIYSVQVDLGEYLPDWHPWEDY
                     RQSYAAREDQGGGVVLTFIHELDYLHWLFGKPKEVYAIGGHRTPLEITVEDTALITFE
                     TELGICVQLRMDYWRKPPVRHMNVVAEKAIIDWDYPDRRTTLKKEGKIADEIELPQTW
                     DRNELFLSMMKEFLEVISHGGSPRVTLRDGIDVLKTALAVKKSLQTGEKIALEEGRDI
                     KRVAL"
     gene            complement(9919..10632)
                     /locus_tag="VFI43_02815"
     CDS             complement(9919..10632)
                     /locus_tag="VFI43_02815"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HET7061090.1"
                     /translation="MLIYSLIPARGGSKGVPHKNIRPLQGKPLIVHSIEISLKCPSIQ
                     RTFVSTDSQQIAEVARNAGAEAPFQRPPELAQDDTRDLPVFQHFLGWLAQNDIPLPDA
                     IFQFRPTSPSRSVSKIEEAVELLKRHPEADSVRGVVEPGQNPYKMWTVADGLMHPLLS
                     ISGVAEPFNEPRQSLPKVYWQVGYLDLIRTQTILQQNSLTGIRILPLKIDSKDSIDID
                     DEFSFQLAEFLMEKRGSAT"
     gene            10961..12361
                     /locus_tag="VFI43_02820"
     CDS             10961..12361
                     /locus_tag="VFI43_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface carbohydrate biosynthesis protein"
                     /protein_id="HET7061091.1"
                     /translation="MASPASTLIIPVENQVRELDAKLLLSCIAAERGFPVIIGSRAFI
                     HFEVASIPRGVYLAKSMRSLSSSMFKILRMLGHEIVAWEEEALVHPPADTYFTLRLSP
                     STITKVSHVFAWGQENVDLLEQYPHLPANMPIHVTGNPRGDILRPEMRAYFDVEVKRL
                     RDLYGDFILINTNFTDVNPFIPSIGLFLPAEDGGKGTRRGQAGIGMSDEFAEGLWDHK
                     QAILEDFKKLIPALARAFPRTTIVVRPHPSESFKVYNDIAAKYDHVKVISEGNVIPWL
                     LASKTMVHNGCTTGLEAYALGVPAISYLSTFNEYYDYDFQGLPTKLSYQSFSFDELET
                     TLSGILKGELGVAESEERKALIDYYLSAQDGPLACERIVDVLEKSGYATGQPPPSPPA
                     TYAQGWMFTKVKATLTNLNMRRPGPNRLSYHDHRFPEVSVADIEQKIARFGRLLNRFD
                     DIKVKQHSRHLFRINA"
     gene            12491..13369
                     /locus_tag="VFI43_02825"
     CDS             12491..13369
                     /locus_tag="VFI43_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET7061092.1"
                     /translation="MKTPKVIAEIGCNHKGDIEIAHEMIGIAATFAKCDFVKFQKRSN
                     RELLTPEEYDARHPNPQNSYGDSYGAHREFLEFDVEQHRQLQQWCSEFGIGYSTSVWD
                     LTSAKEIASLNPEFIKIPSACNLNFPMLRYLAMEYGGDIHISVGMTTPAEQEQIVQLM
                     EECNAARRVVLYACTSGYPVPFEQVCLLEIQALNEKFGSRVKEVGFSGHHLGIAIDIA
                     TVVLGASWIERHFTLDRTWKGTDHAASLEPDGLRRLVRDVRNVCKTLKLKENDMTEIE
                     LTQRDKLKRVQGVHFS"
     gene            13369..14568
                     /locus_tag="VFI43_02830"
     CDS             13369..14568
                     /locus_tag="VFI43_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET7061093.1"
                     /translation="MRWVAVISSGEGGGEKNIARRMAGRPLFAWALEQAIASDCFDDI
                     YIATGSPEICEQLLGELPWAQGSIKILDDSALAFAVGRSSDNMLLPLREDSPFDVLCM
                     IGARCPLTHADDFRTAKDKFLAGNFDSLVTTVRLKRPLWLPRGAPITAAPAGVSGSDG
                     AGEYLMENGAFSIMRATALGKDELRLNDRIGIHEMPLETAIEITDEAGWFAVEQLLSI
                     RKLVSTKARAQQIGVLVLDVDGTLTDAGMYYGPGGEALKKFNTRDAHGLQLLRENGIT
                     VCVITTEESPSVQARMRKLQIEEYYPGVGNKFPLLLDLAKRWGVSLQNIGYVGDDLSD
                     LECLRKVGCAFCPADAVPQVKQQVHHICEQRGGHGAVREVCDLILEAKESATGNISAP
                     TLASTSA"
BASE COUNT         3468 a         4000 c         4011 g         3543 t
ORIGIN      
        1 attgtctcct gctggtcatg aacaatagcg gaacttcgtc ctatggaaat aatgtcttac
       61 acgtacgcag ggcgcactcg ataaggggga tgcaaagatg ctgccaggaa ggctccgatc
      121 gtcttttttt tcgcacgccc aggtaacgtg tcgagggaga gatacttcgt ctgattctga
      181 ccactgtcgc ggttattgga agacagtggg aggacatcat tctgcagcaa atgacccgtt
      241 gcttgtgcac gcgctccgct agctttccag ccgtattttc tctatgattg cgtacccgca
      301 gaccaccgca ttgcatacac gtgcagcaac agcaagcaag tttccctttt tcaagatcat
      361 caataacagt accgggcagt ttatccacgc gtgacaaagc tcgttctgct gcgccatgga
      421 gaaagcatct caaaccggga tggacacttt accggttggg acgacgttga attgagcgaa
      481 aaaggtgaac gggaggctgt gcgcgccggt acttggctca aggaagcagg cttcaagttc
      541 gacgcatgtt ttacgtcgga gcttaagcgg gctacccgta ccctgcatat cgtattgggc
      601 gaaatgggcc tggattgcct gcctattcga cgtagctggc gtttgaatga acggcattat
      661 ggggcccttg aaggtatcga tcgtctgagc gccatcagga agttcgggat ttttcctgtt
      721 cttgactctc aacttcgctt taacgcgtct cccccacctc tggatccctc cgatgtacgg
      781 tttcctggca accagtcgcg ctattccggt atcgacaaaa gtgaattgcc ccgatccgag
      841 agcatggggc aagcactctt gcgggtattg ccgtactggc gcgaaacaat tgtgccggaa
      901 ctgcagggtg gaaaacaagt tctcgtcgtc gctcacaagc atatcctgcg tgccctgatc
      961 atgcagctcg acagcttgtc cgctgtacaa cttataaaac tgtccatagc gaacggacgg
     1021 cctctggtat acgaactgga caaaaagttg aaccccattc gccactatta tgcggatcaa
     1081 aaagacggct ttagataagc tttaccgtcc cgtatatgcc agttagccgc aggcaagact
     1141 gcccgccgct taattcccga atgcacctac acaatttggc acctttgaca caattctcga
     1201 tcgtcatacc cacgctcaac gaagcggaaa atattgatcc attgctgact cgtctgtttt
     1261 cgccagagct tgagaagaag agttttgaag ttatcatcgt tgacgatggc tcgacagacg
     1321 acactcagga taaggtaagg gcctgggaaa cccggaagaa cgtccgcctg gtcgcgcgcc
     1381 atggaaaacc ggatctcacc gcttccgtac ttgaaggtgt agcgctcgcg cgcggcgagg
     1441 tgattgttgt catggacgct gatctcagtc acccgcctga acggttgctg gccctgactg
     1501 cgcctatcct tgaaggaacc cacgacgttg cgatcggaag ccgctatgta cccggaggca
     1561 gcacggaggg ttggccggta catcgccaat ggttatcccg gctcggaggg tggctcgccc
     1621 gtcccctctg cgatgtaaat gatgcgactt cgggattttt tgcattccgc agggatctcg
     1681 ccgcaacaat tgctgaacgt gcgcgaggct acaaaattct gcttgagctg ctgatggcca
     1741 gccagggaaa gcttcgggtg atcgaggttc ccatctgttt tacggaccgt acccgaggca
     1801 agtcaaagtt gtctttttcg caccagtgga catacctgca gcgtttgatg acgcttgccg
     1861 gaggaacggt ctcagtaggc acggccagcc ggttcgccgc ggtcggactg ttcggcgtgg
     1921 tcgtcgatgc gctggtattt caactattga tgagtaatgg aactcggctt gcgacagccc
     1981 atatcgtcag cttccttgtt gcagcttccg taaactatac gctcaattcg aagtggtcgt
     2041 tccgtcccca tcatcaaggt cgcctgcagt ggcagcaatt cagccgctat ctgacggtcg
     2101 ccgtattcgc gctccttatg cggggaggcg tattggccct gctcgtctat aactggcacg
     2161 ttccatcctt tctatcgatt tttcctgcca ttgcagcgac ggctgttatc aactacctcg
     2221 ggtctgcttt ctacgttttc cctgcggagc aggacaggcc ctctcccgat gtcagctggc
     2281 gggttgcttc gttcggaatc attgttttta gcctcttgct gcgcctgatc tatctcgaca
     2341 tggtagagct tattccagat gaggcatatt actggaatta tgcgcagcat atggatttga
     2401 gtttctacga ccacccaccc atggtcgcat ggctgatctg gctcggcacc tcgatctttg
     2461 gaaacagcga gtttggcgtt cgattcgggg cgttttgttg cggtctcgtt acgatgggct
     2521 acatgtacga gttggcgcgc aatctctacg ataaaacaac cgctattcgt acagtgctcc
     2581 tgcttgccgt gctgccattc ttttttgcca caggctctgt catgacagcg gatgcgccac
     2641 tcgtcgccgc gtgggcggcg acgctgtatt acatggagcg tgccctgctt gccgatcgca
     2701 gactcgcgtg gctgggcatg ggaatcgcgt tcggccttgg catgctgtcg aagtacacgc
     2761 ttgcgcttct tgggcttgcc gccctggttt ttgtgatcgc agacccgtcg tcccgccgct
     2821 ggctgcgccg tccgcacccc tacctcgcgg caatgctcgc ctttctgctg ttcttgccgg
     2881 tgatcatctg gaatatgagg cacaactggg tttcgctttc gttccagtcg agccggatca
     2941 aaggcgttgg cgacgatcag ttttccgtgc acctgctgct ccttcatctt atggtactgc
     3001 tgacgccagt gggacttgtg gccgcattcg ttgcactggc gccgaatcgc gacagaaaca
     3061 ggagtttgtc tgcggacagg cgccagctgt tcatctggtc attcgccggg gtgcctttgg
     3121 cagtatttgt catgcttagc atttccgact cgctgcgctt ccactggaca ggaccgttgt
     3181 ggctggcgtt gctgcctaca atagcgtgga tgatggggca gacgagttcc gccagcgcaa
     3241 ttgtccggcg gctgcaggcg gtttggaaac ccactatagc tatttgcctc tttctctatg
     3301 ctttttcaat gcattatctc gtgcttggca tacctgggat tccataccag gggatttaca
     3361 agggctttcc cgaacattac ttctggcggg aagcaacgaa gggcgttgaa caaatcgcgg
     3421 aagaggtgcg acaggagacc ggccaagagc ccatcgtcgt gggcatgagc aaatggtcag
     3481 tggcaagtat catgagcttc tacaaccgtg gaaaacccat ggagatccgc tctcgcaaca
     3541 tgttcggcga cagtggcgca atgtacgatt tctggtatcc gtcggaaccg cccacggcac
     3601 ggcccattat cctcgttgga atgtggcctc ataatctcga acgaacgcgg gaagaccgtg
     3661 acatcggaac gatgcttgtg gagcccggtc cggtaaaaga tagggcaatc tcacaggatg
     3721 gcaaaccgct acgtaacgta tattatcgcg tggcccatgg atatgtcggg aatacgaaac
     3781 tgggagaatc ccgcgtggcc gacgtaaagg ggaaaccgga taactgaatt gacggccaaa
     3841 cgccacgtcc tgcccaccat cagcaggaac aaggacgtat acgggttatg tcgaggtcag
     3901 actgctatgg tttcagagcc ccgagcttct tctttaccgg atatggcaac agcggtaaag
     3961 gcaagaagga aggtgagaat tttggtatac agcgcgaaag tgtctgccag gcccgaaata
     4021 aaaatgaaac atatcagggc ggccccaaga acgggcagac catgacgctt aaacgtttga
     4081 aaccaactcc acagaagaaa agtgaaagac agaacaccaa gcgcgcctag atgcatcccc
     4141 atctcgatcc agtcgttatg gtagtgagag gtttccatgt actgcggcag atccttgtaa
     4201 accccatctt tgtatgtctt caccgtcttt tcaaaatagc tttcgggcgc cccggtgcca
     4261 tgtccaaaca ggggcttctc ggcaattcca cgcacaccaa cgtcccatag cgcgagccgg
     4321 tatcccagac tggtatcata ttttcccagc cgggcaagct caatgtcggc gctcagctgg
     4381 gagagtctcg attgcaggac gccactgtat gaaaacgcta tgattatggc ggccagtaat
     4441 cctgtaacgg ccaacagcac ttttttctcc gttttatagc gagcaacaac gagtaatgct
     4501 gaaccgagaa gagtggccag aagcgcgccc cgcccatatt cgttgaactg taggtatagc
     4561 aacagcaatg ccagcagcca acaccccgct tttatttttt tgcctgtttc ctcgccggcc
     4621 gcgtagattg ataggatgac accgattcca agcattgacg aaaaagtcag atagctcatt
     4681 ccgagtagag ggactccttg ctccccaagc aggcgatggt aaaccccgat cagcagtacc
     4741 atggcatagc cggtgacgag accggttatg gcccagaata agcgctcttt attcaaaagg
     4801 gacagaaagg ggatgaagac cagaaatgca aaatacctct tccacttcaa acgccccgat
     4861 tccggatatt ccccccacag gagcccgagc atcaacagtg cgcacagaat gagtatcgcc
     4921 agtacgagag gttctttgat agcccgatcc agccgccgag ggccattatc gagaatccat
     4981 gcaacggcta gcaggatacc cgctcctaga ctcagccatt tagcccataa cgctatgccg
     5041 aaaaaaaaca gcgcaaactt gacaagagcg ttttgccacg cgcgcgcact tcgtatcagt
     5101 tcggatgcgc ttgccggctc atcatcatgt gcgaccggct gtctcgcgat ctcgtgagtc
     5161 atatcgtgct gaagccgcaa gtatctttat gcgttaaaag tgcgatgagc tgtgagcggc
     5221 ttaaaaagag gggagccttc aaaaaaaaag gaggatgcac gcattacggt gtagagccca
     5281 cggcccgatt gcccggaaac cgggaaagtc ccaattattt cgtctctttc cggctgcatt
     5341 tgcaatattg ttgcttacct cgtagtattt atggccgcgt ttgcatccct ggacaatacc
     5401 cgcctgcaca tccgcctgat tccagtcctc tcgaagcagt taaccttgat tatcctgaag
     5461 cgttgcccgc agcagcatta tcagcgcgag tgtgtgcaag aacattaaat aatgaaagcc
     5521 cttttttttc ttcgtcatta taacgacatc gaccatatca cgccggtgat ctcaaagtgg
     5581 gtggaagctg gccacagctg tgatgtcatc ctggttggca acccgaagtt tcgcagcgat
     5641 taccgtatca ggtttgtcag cagtctcagg aatgtaacgg tcgacgtcat tcgcgatgtg
     5701 ttgccttcga ccgaattcct tcgatggcga ctgcagatgc ttctcctcag caacagtctg
     5761 cgccgctcgt tcattggacc gctcgtaagc gccctggctc gcttttacga cgctaaacgg
     5821 cgcgagccgg cttggcgaga ttccgcgcag cgcttgcttg aacgcagctt caaggacgtt
     5881 gaggacggcg tggtggtatt cgactggatt accaaagatt caccggtttc tgtcgagtgg
     5941 gtagagcatg tggtagcaac cgcgcggagc agaggcatcg gagcggtgtc actgccccac
     6001 ggtgacagtc ctcacgcaaa ccggatgata cgggagggtg aatggacgct taagcctgat
     6061 accttatttt cggcagcaaa gatattcgat aagctcgtga tccccaacga actatgtgcc
     6121 ggtcgctttc gcccctttct tgaaaacagt gctatcgaag ttctcggttc cccgcgctac
     6181 tgtgatgagt ggctcgccag gctggcaacg ttgtcaccgc ccgccgtatt accatccagg
     6241 ccgggtattc ggctcaagat cgtgcttttc ctgcgaaaaa gcaactttac aacgttctgg
     6301 gaagaagtcg gtgaagtgat tggcatgatt tcaacttttc ctgacgtcga attgatcatc
     6361 aagccccata cccgtggtgg ctggagacaa ccgcttaccc ggaattcttc ccttcgtcac
     6421 atgagaaacg ttagtgtcgc agcggacaat gttcattcga tccccctgat gaactgggcg
     6481 gatgtaatta tcgaccttgc cacttctgtg gtattcgaag cagtcaaggc aaagaaaccg
     6541 gtgctggcgg cagattatct gcactgcgga cgctccgccc tcgcttacta cattccggag
     6601 accgaactga aatgccggga tgacgtgtac cgcaagatcg atgagtttct tacgattggc
     6661 tgtgaatcgt tttacgtgga ggaacaccgg cagcgtttca tcgaggagat gcttcatgtc
     6721 ggcggagcgg acgttttgcc acgttatgtt gcactgctcg agtcccaggc ccgcgataaa
     6781 cgtggaactg gcgaagttct catgcttgat gggcgaggca gcatcgccga gcagacatcc
     6841 acttgattgg gaatagtgac ttcaggacag cagagggtat tacagtcggc gcgttcaccg
     6901 gaaatccatt tcggcggacc tgctgagctc cctggccacc tacgcaacgt gctggcagaa
     6961 cgtattgcgg ccgtacccgg cggtggctca atcgactggg ttacttatta ctttcgggac
     7021 ctgcaacttg cccaagcgct cattaaggct aaaaacaggg gtgtcagggt gacggtaatt
     7081 ctcgaaggca ggccgcgcat tcctcacgca aatgattcgg tgattgccat gctttcggga
     7141 gcggacggac tcggagaagg gtttcggctg gtagcgctcc cgggaatacc atcgccgtcc
     7201 acgaaatcct ggaagccgca gttgcatgaa aagctctatt gtttttccca tccccgaccc
     7261 atcgccttca tcggctcatt caatccttca ggaaacatgc ccgacgaaga ccccgagatc
     7321 atacgggaga taggggatca ggatcgaggc tacaatgtcc tggtcggttt cattgagcct
     7381 gacctggttg aaaaactcgt ggatcacgcg aggcaaattc atcaggtatc accgagcatc
     7441 ttttaccggt tttcgcccga tgcaaaccga gcgatccatg gcagcgacac ggcgattcac
     7501 ttctggccga gagcgaaccc tcatcctgtc atccgattcc tgcatcaggt atcgagcaag
     7561 gctcgtgtgc gtgttgttgc ttcacatatc agaacggcga gcgccgtgga aaccatgata
     7621 acgctggcca gacgcggagt cgccctcgaa atatttgccg aggcaacctg cagacgggtg
     7681 acgccaaggg tcgaacgact cctgtcctca gccggaatcc ggttcaggcg gatcaggatc
     7741 agtggcgaac tgcctatgca cctcaagttc gtgctaattg aggacggtaa tcaggtgaac
     7801 tcaatttttg gtagtttcaa ctggaccaag ccttcgttct ggctaaatca tgagattgcc
     7861 gctatttcca gtaatcgcga gatctttgag gcgtttgcag agcgctggga catgctggaa
     7921 agcgaaaaat cggataagac gtaaagcgcg tcgctaaagc ttcccaatcg gatataactg
     7981 cagtatgacg ttccacagca agtaggctga ttcttctgct ttagcatcag acgcagcttc
     8041 gaccattatg gctcgtcatc tgatcttctt cacagtgccg tccacccgcc atcaacaacc
     8101 aggtttgcac ctgtcatata gcttgaggca tccgaagcaa gaaaaataat cgcgccccgg
     8161 tattcgtttt tttccgacat cctgcctagg atggttcggg ccgaataatt ggcggcgaac
     8221 tccttgtcat gctggttcca gatgccgctt ggggtgagca tgtttacccg aatggatgta
     8281 ttcatgtagt aagcggcgag aaactttgtc agtcccaaca ggcctgactt gcatacggag
     8341 tagaccgccg gcttgtacgt ctgctgctcc tgcccagctt tcctgtaaat acgttgatca
     8401 ggccctacga gcccgtagtt ggatcccaaa aaaataatcg aaccctttcc cgatttttcc
     8461 atcaccttgc aggtcgcttg agcaagcagg aaggctcccg tgagattgac ttcaagcgaa
     8521 gtctgccaga gctcaactgg aaattcggta aatcttgaaa agcgcgaggt atccgatcct
     8581 ggttcgaatt tgggatcgat agccgcgctg tgaattacgg cgtcaatccg gttaaaccgt
     8641 gtttcgcaag cttcggcaat cgatcggcag gattcggggg aaacgacatc caggacgtgt
     8701 gcaagcactt ctccatcggg gtaggcggag actatttcac ctttcaagga ttcgagcttg
     8761 tcactgtcta aatcccccag caccaggtga gcccccgcgg acgcggcagc cagggcgaac
     8821 tccctgccca gaagacccga ggctccactg atgagcacga cccgtccggc cagatcgaat
     8881 ggcgttcctt tgtaagcctg actcataacg caactctctt tatatcgcga ccctcttcta
     8941 gtgcaatctt ttcgcccgtc tgaagggatt ttttgactgc caaagccgtc ttcagcacat
     9001 cgataccgtc ccgcaacgta acgcggggag aacctccatg ggagataact tcaagaaatt
     9061 ctttcatcat cgagagaaac aactcattcc ggtcccaggt ttggggcagt tcgatttcat
     9121 cggcgatttt cccctctttt ttcagagtgg tgcgtcggtc gggataatcc caatcgatga
     9181 ttgctttttc cgcgacgaca ttcatatggc gaacaggcgg cttgcgccaa taatccatgc
     9241 gaagctgaac acaaatgccc aactcggtct cgaaagtgat tagagcggta tcctccactg
     9301 tgatttcaag gggtgtgcga tggcccccga tcgcatagac ttccttgggt ttcccgaaaa
     9361 gccagtgaag ataatccagt tcatgaatga aggttaaaac aacacctccg ccttgatcct
     9421 cccgggctgc gtagctttgc cggtaatctt cccagggatg ccagtcaggt aaatactccc
     9481 cgagatccac ctgcaccgaa tagatttgac caagtttatc ctggcgaacc cactcttcaa
     9541 ttttgcgcaa gccggggtgc attctaagct ggcatccgac cagcaccttg gaatcgttgg
     9601 tcgaaaggta gggggccagt tcgaacagag ggccattctc gtcggccagc ggtttttcta
     9661 cataaacatg tgaacccgct tcaagcgcgc gaaccagtgt ctcaaggtga agcgatgtcg
     9721 ggttggcgac cacgacgaga tcatatcctt ggctgagagc gagatcgaga tcgtgaaaga
     9781 cagtaatgtc cttcgggacg attgcatgga aaggaaccag attgcgactg cgcaccactg
     9841 caagttcctg cacgccgagg gcgtgcagat tgttgaggtg cctgacgccg attgacccca
     9901 aaccgactac cagcgccttc aagtagcgct cccccgtttt tccatcaaaa actcggcaag
     9961 ctgaaatgag aactcatcat cgatatcgat cgagtccttg ctgtcgatct tgagcggcag
    10021 gatgcgtatt ccggtgagcg aattctgctg caagatggtc tgagtgcgaa tcaggtcgag
    10081 ataccccact tgccaatata ctttaggcaa gctttgcctg ggctcgttaa agggctcggc
    10141 aacgccggaa atgcttagca agggatgcat caaaccatct gcgacggtcc acatcttgta
    10201 cggattctgc ccgggttcta cgacgcctcg aaccgaatct gcctccgggt gtctcttgag
    10261 caactcaacc gcttcttcta tctttgaaac gctacgggag ggagacgtag ggcgaaactg
    10321 gaaaatggcg tcagggagcg gaatatcgtt ttgcgccaac caaccgagaa aatgctgaaa
    10381 tacaggtaaa tcgcgcgtat catcctgcgc cagttcaggc ggtcgctgga acggagcttc
    10441 cgcacctgcg tttctagcga cctcggcaat ttgctgcgaa tcagtgctga cgaaggttcg
    10501 ctgaatggaa ggacatttga gtgagatttc tattgaatga acgatgagcg gcttgccttg
    10561 caaaggtcgt atattcttgt ggggtacgcc ttttgaccct cctcgtgcgg gaatcaggga
    10621 ataaatcaac atctatgata agctgccaca cttccattca gcgagcccga gcggccctgc
    10681 ggcccgcaat gatgcgcttg ggataccgca ttgtcaagca accgtccttc gatgatgttc
    10741 ccttgtccta cgctgccagc cgcgctaaca tccatcgctt gccttccaga gaatgcacgg
    10801 ccggtccacc ggcaagtccc atatccttgt tattgcctga gaatgattgc ctgtactgca
    10861 tacaatgcga taataacggg tttgaaatcg gttactggaa gatggaattg aaaggctgga
    10921 attgaaaggc tggaaataga caagattcct gaattagtat atggcaagcc ccgcttcaac
    10981 ccttattatt cccgttgaaa accaggttcg ggaactcgac gccaagctcc tgctctcctg
    11041 catcgcagcg gagcgcggct ttccggttat tatcgggtcc cgcgcattta ttcatttcga
    11101 ggtcgcatcg atcccgcggg gtgtctacct ggctaaaagc atgcgtagcc tgagcagctc
    11161 catgttcaaa attctccgca tgttggggca tgagatcgtg gcttgggagg aagaggccct
    11221 ggttcaccct cccgcggata cctactttac gcttcggctc tccccgtcta cgataacaaa
    11281 ggtttcccac gtgttcgcgt ggggacagga aaacgtggat ttgctcgagc agtatccgca
    11341 ccttcccgcg aatatgccca ttcatgttac gggaaacccg agaggcgaca tattgcgtcc
    11401 ggagatgcgc gcctatttcg atgtcgaggt aaagagatta cgggacctgt atggggattt
    11461 catcctcatc aacacgaact tcactgatgt aaatcctttc attcccagca tcggcctgtt
    11521 cttgcccgcc gaggacggag gcaagggcac gcgccgcgga caagccggga ttggaatgag
    11581 cgacgagttc gccgagggat tatgggatca taaacaggcc atactggaag acttcaaaaa
    11641 gctcattccc gccttggcgc gcgcttttcc ccgaacgaca atcgttgtac gcccgcatcc
    11701 aagcgagagt ttcaaggtct acaatgacat cgccgcaaaa tatgatcacg tgaaagtcat
    11761 tagcgaggga aatgtcatcc cctggcttct ggcttccaag acgatggttc acaatggctg
    11821 caccactggg ctcgaggcat atgcgctcgg tgtcccagcg atatcctatc tttccacctt
    11881 caacgaatat tacgattatg actttcaggg gctgccgacc aagctgagct atcagtcctt
    11941 cagcttcgat gagctggaga cgactttgtc cgggattctg aaaggtgaac tgggagttgc
    12001 ggaaagcgag gaacgcaagg cgctgatcga ttattatttg tcggcccagg atggacccct
    12061 ggcttgcgag cggatcgtcg atgtcctgga aaaaagcggc tacgccaccg ggcaaccacc
    12121 tcccagtccc ccggccacct atgctcaagg gtggatgttc accaaggtca aggcaaccct
    12181 gacaaacctt aacatgcgac gccctggccc gaacaggctt tcttatcacg accaccgttt
    12241 tcccgaggtt tcggtagccg acatagaaca aaaaattgcc cgctttggtc gcttgctgaa
    12301 tcgtttcgac gacataaagg tcaaacaaca ctcccggcac ctgttcagga ttaacgcttg
    12361 acaagctgag caagtcatcc cccgtttttt caaaagagtt cccgattcag tatgctatag
    12421 tggcggcctg caaaaacaca ggcacgaatt tagtcaattt tccctttcca gaggactgct
    12481 cgcacgtttt atgaaaacac caaaagtcat cgctgaaata ggttgtaatc ataagggaga
    12541 catcgaaatt gcccatgaaa tgataggaat cgctgctaca tttgcgaaat gcgatttcgt
    12601 caagtttcaa aaacgctcaa acagggagct tctgactcct gaagaatacg atgcacgcca
    12661 tcccaatcct cagaacagtt atggggacag ctatggcgcg caccgcgagt tccttgaatt
    12721 cgatgtagaa cagcatcgcc agttgcagca gtggtgcagc gagttcggta tcgggtattc
    12781 gacatccgta tgggacctga cctcggcaaa agaaatcgcc agccttaacc ccgaattcat
    12841 caagataccc tcggcctgca acctcaactt tcccatgctg cgttaccttg ctatggaata
    12901 cggtggggat atacatatat cggtagggat gacgacgcct gcggagcagg agcagatcgt
    12961 gcagctgatg gaggaatgca atgcggctcg gcgggtggtg ctatacgcct gcacttccgg
    13021 ttatccggtt cctttcgaac aggtctgcct gctcgaaatc caggcgctca atgagaagtt
    13081 cgggtcccgc gtcaaggagg tcggattttc gggccaccac ctcggcattg cgattgacat
    13141 tgccacggtc gtgctaggcg cctcctggat tgaacggcac tttacgctgg accgcacatg
    13201 gaaggggacc gaccacgcag cctcgttgga gccggatggg ttgcgccgcc tcgttcgcga
    13261 cgttcgaaac gtctgcaaga ccttgaaact caaggaaaac gacatgacgg aaatcgaact
    13321 aacgcagcgt gataagctta aacgcgtgca aggcgttcat ttcagctgat gcgctgggta
    13381 gccgtcattt cgtctggcga agggggcggt gaaaaaaaca ttgcacgcag gatggcaggg
    13441 cgccccctgt ttgcctgggc gctcgaacaa gccattgcat cggattgttt cgatgatatt
    13501 tatatcgcta ctggatctcc ggaaatttgc gagcaactat tgggtgagtt gccctgggcg
    13561 cagggatcca tcaagatcct ggatgactcg gcgctcgctt ttgcggtcgg gaggagcagc
    13621 gacaatatgc tccttccact tcgggaagac tctccatttg atgtcctatg catgatcggg
    13681 gcgcggtgcc cactgacgca tgcagacgat ttccgcacag ccaaggataa gtttcttgcg
    13741 ggaaacttcg attcactggt caccactgtg cgcctaaagc gcccgctgtg gctacctcga
    13801 ggtgcaccga taacggccgc tccggcagga gtttctggtt cggacggcgc aggggaatac
    13861 ctgatggaga atggggcttt ttctatcatg cgggcaacgg ctcttggaaa ggacgagttg
    13921 cgcctgaatg atcgtattgg cattcatgaa atgccgctgg aaaccgcaat tgaaataacg
    13981 gatgaagcag gctggtttgc agtggagcag cttctgtcca tccgaaaact tgtgtcaaca
    14041 aaagcccgtg cccagcagat cggcgtcctg gtgctggatg tggacggtac gcttaccgat
    14101 gcggggatgt attacgggcc aggcggtgag gctttaaaga aattcaatac ccgcgatgcc
    14161 cacggactgc aactactgcg ggagaatgga attaccgttt gcgtcatcac caccgaagaa
    14221 agcccatccg tccaagcaag gatgagaaag cttcagattg aagagtatta cccgggcgtc
    14281 ggaaataaat ttccgcttct tctggacctt gccaaacggt ggggtgtttc gctccagaac
    14341 attggctatg tcggtgacga tctcagcgac ctcgagtgcc tgaggaaagt cggttgtgct
    14401 ttctgtccgg cggacgctgt tccgcaagta aaacagcagg tgcatcacat ctgcgaacaa
    14461 cggggggggc atggagcggt acgggaagtg tgcgatctga ttcttgaagc taaagagagc
    14521 gctaccggca atatctccgc accgacgctt gcgtcgacgt ctgcttagtc gggattaagc
    14581 atcactttgc gggcggctga tagaaattta accgccccag gttttgagga ggctccaaca
    14641 tctgagagca tggggccatg aaaaatcaaa caggttttcc ctcgaagtgc gtgagcgccg
    14701 tacgcatggg tgcgcatagt gttcgaacat cgcactatgg ctatcccaat cctcttgatt
    14761 ggaaagaact ttctcctaag ggaaacgctt agaaaagaat gagggtttcc ccaatatacc
    14821 catggtattg actgaggtag gatgaagtca ctttcactct atttgagttg tcgccatgaa
    14881 aacaaatttt ggaaatatct tggcagcatt gtccatgatt ggtgtcctgg cgtcagtaag
    14941 ctttccggtt gtggctgccc agcccatcga taccccggaa attcgtgctg cggctcagag
    15001 tgctgccacc cgcgatgagc at
//
LOCUS       DASSHJ010000070         9466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1154119,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000070 DASSHJ010000000
VERSION     DASSHJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154119"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VFI43_02835"
     CDS             complement(<1..154)
                     /locus_tag="VFI43_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061094.1"
                     /translation="MNATVPNEAQFSSFTSKAAQVDKAATKPLPKSRKVYIQGSHPDV
                     RVPMREI"
     gene            340..1005
                     /locus_tag="VFI43_02840"
     CDS             340..1005
                     /locus_tag="VFI43_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET7061095.1"
                     /translation="MDLEQSRFNMVEQQIRPWDVLDQEVLNLLFEVRREEFVPAAYRS
                     LAFVDMEIPLGHGAVMLAPKLEARMLQELQIKNTDKILEIGSGSGYFTALLASKGGYV
                     HSLEIVPELKAMAERNLKRHGIGNVSLEVGDGARGWPEHAPYDAIVLTGSTPVLPEAF
                     QKSLRPGGRLLAVVGDAPAMEAMLVNCIAQDEKGGLYNRVSLFETVVSPLKNAEQPER
                     FVF"
     gene            1117..2436
                     /locus_tag="VFI43_02845"
     CDS             1117..2436
                     /locus_tag="VFI43_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HET7061096.1"
                     /translation="MKLARYIGPLFIAGVLLHGPSYGRDLMAVYREALEQDAQYRSAH
                     AAYQAAQEKLPQGRAGLLPTLTFSGIRRRQLIDRMGAPERTIDNQSLTITATQPIYRK
                     ENFVVYEQAKIQLAQADSQFILASQDLILRVAQAYFDILTSHVNVEVAEAQKKAIGEQ
                     LEQAKRNFQVGTSTIVDTYEAQARYDLTLSQEIAARNDLEIRKRTLQQIIGRMPDELI
                     RPNELTFDLLTLKHPRMEDWITLAEQNNLTLKIQQAIYNIAQQDVERAKAGHYPTLDL
                     VAVYSDQKGVGGTITGQGINLTSQEIGLQLNVPIFQGFAVQSRVREALANQERAHQDL
                     NNTRRNSVLLVSQQYLNVTNGMAQVKALQQAVASSKSQLESTRLGRDVGVRTEVDVLN
                     AQQLFYSARRDLAQARYNLLMSRLRLEAEAGELDEDDLRQINEVLVQ"
     gene            2625..4271
                     /locus_tag="VFI43_02850"
     CDS             2625..4271
                     /locus_tag="VFI43_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7061097.1"
                     /translation="MNQPSESHSYWAQRPILSIADRVAAIGDTRPTDMDNAVLDAKTF
                     DAIEADEMFESLDHTGTHIGKCVLYRSLARPGTNPGVIREKQEAVRELAANPGLRDSL
                     QRLIDEAGRGEQALYDLLYGTFTGGLAVDNSRMGKDGMEFSGYGYDQFIDGTGFVVDV
                     VETVKTLPPPQSKYLKTLFDAIRGFERSRVYSLISGPVYLSEGKFKTREEKSGYMPLP
                     RFRPSVIKPIPMLFALGAISVALYVFQTLLASFGIAYLGYGILVLIVPLLPVLLIAIG
                     ASDRDSVIYPLRKLFKHSPELAQWLDTLGMIDELNCFHRYSVAFGGDMTLPDILESER
                     HSLRASEARNPILGRSNPTYVPNDILLGDPERLLVITGPNSGGKTAYCKTVVQIQLLG
                     QIGCYVPADAAQLVLAEHIFYQVPDPGHLDEGMGRFGHELKRTREIFFNSTARSLVVL
                     DELSEGTTFEEKMELSEYVLMGFHELGASTLLVTHNHELCGRLQEKGIGRYLQGEFLA
                     QGPTYRLIPGVSRVSHADRVAAALGFSKEDVEKHLAERRS"
     gene            4434..5423
                     /gene="hpnA"
                     /locus_tag="VFI43_02855"
     CDS             4434..5423
                     /gene="hpnA"
                     /locus_tag="VFI43_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HET7061098.1"
                     /translation="MKSLVTGGNGFVGSAVARRLLDAGYEVRCLVRPGSDRRNLHSLP
                     IEIVEGDLRSVPSLKRAVAGCDNLFHVAADYRLWVPNPGTMYDINVKGTEALILAAAE
                     AGMKRMIYTSSVATLGPSRDGVPANENTPSTLESMTGHYKRSKFMAEQVVQHITREHK
                     LSIVIVNPSTPVGPRDVRPTPTGRIVVETLLGRMPAYVNTGLNVAHTDDIAHGHFLAY
                     KHGKPGERYILGGENMTLLQILRTIDEIQGRRRTRFNLPANLVLPAAWLMEKIAAVTH
                     VEPAATVDGVRMAKKMMFYSSDKAVRELGYRFRPAAEALRDATNWFRENGYCG"
     gene            complement(5459..6751)
                     /locus_tag="VFI43_02860"
     CDS             complement(5459..6751)
                     /locus_tag="VFI43_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7061099.1"
                     /translation="MNRIIAVAALAGLTGLGINVSISLPAANEGLPVAMDAAEDVAAQ
                     RKWQQARGAYLAKIGNCMGCHTAQPGQPYAGGHNLDTPIGTFVTPNITPDRETGIGLW
                     SEDDFWRALHEGKRRDGSALYPAFPYTEYTKITREDSNAIFAYLQSLPPVQQHNPPNQ
                     VRFPFNLRPLIYLWRIVYFDEGIYRAKSDKSKEWNRGAYLIQGLGHCNACHTSRNPLG
                     GSRDGALGGGQIMGSNWYAPSLTSLQEASTSDWQVPEILNLLRTGLSSRAAVAGPMAD
                     VTSQSLQYLTHDDARAMAVYLQSLPRTAPHSRGVAPELTQEVDEQLREGGKIYETYCQ
                     DCHGRFGEGAPGAYPALAGNRGVTMASPVNAIRSILYGGYGPVTESEPRPYGMPPFSQ
                     ILYDKEIALVLSYIRNAWGNRGSLVTAVQVDRSRKGVQ"
     gene            complement(6748..7503)
                     /locus_tag="VFI43_02865"
     CDS             complement(6748..7503)
                     /locus_tag="VFI43_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HET7061100.1"
                     /translation="MRSFLLIMLITCLFFNLSATATAIPPAKLPGNKVPNTMVERVKA
                     CVACHGPEDKEGRDAYYPRIAGKPEGYLFNQLRNFRDGRRYYQPMAWLLKGLPDQYLK
                     EMAVYFASLEQAFPPPEPMTLSSAEAERVRRLIYEGDAARNIPGCVECHGKDLMGTAP
                     FIPGLLGLPRVYIIAQFGNWQNGGLIRGQTPDCMSEIAKQLAPEEAVGVAKWLAAQPV
                     SEIAPRPGSPAATLPQKLAKRCSTIIRPATQQK"
     gene            complement(7596..7907)
                     /locus_tag="VFI43_02870"
     CDS             complement(7596..7907)
                     /locus_tag="VFI43_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET7061101.1"
                     /translation="MKSQNKKESCHAPRTVVQPDKEALIKRLNRIEGQVRGVNKMISE
                     DRYCVDILNQVAALQSALDAVAMLLLESHTHGCMQGAIKSGKGDEAIDELMAVVRKFA
                     R"
     gene            complement(7891..8100)
                     /locus_tag="VFI43_02875"
     CDS             complement(7891..8100)
                     /locus_tag="VFI43_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET7061102.1"
                     /translation="MQTEVIKVNGMTCQGCVNSVKNVLEKIPGVQNADVSLEQKQVKI
                     QYDAGKTGAEQFKNAIEGAGFEVAK"
     gene            complement(8139..>9466)
                     /locus_tag="VFI43_02880"
     CDS             complement(8139..>9466)
                     /locus_tag="VFI43_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7061103.1"
                     /translation="YAATVNRQGMLKCRATSVGSHTQLAAIVRLVEEAQGSKAPIQRM
                     ADTISGVFVPIVVIVSAATFAGTWWLTGGFVPALVNAVAVLVIACPCALGLATPTAIM
                     VGMGQGAQAGVLVKNAAALEHAEKIRTLIVDKTGTLTEGEPTVTDVIPVGGTAEHDLI
                     QTAATLEQGSEHPLARAVVEYAARAGVQPGTMKGFTAITGSGISAVIDNVEYLLGSPK
                     FLTEKGAVVDQRLIDALQADGKTVVALSALPGNSPRILGYVAIADRLRATSAAAVHRL
                     RAMDIEVVMLTGDNPATAAAIARQAGIPDYRAEVLPQEKAAQVREMKAGGRCVGMVGD
                     GINDAPALAMADVSFAIGAGSDVAIEAADITLMRNDLMSVADAISLSRATLRKIRQNL
                     FFAFIYNVLGIPLAAMGLLNPVIAGAAMAMSSVSVVSNSLLLKRWRAGA"
BASE COUNT         2098 a         2609 c         2643 g         2116 t
ORIGIN      
        1 tgatttcacg catcggcact cttacatccg ggtgtgaacc ctggatataa accttgcggg
       61 atttgggcaa aggcttcgtt gccgctttat ccacttgcgc cgccttgctt gtgaacgatg
      121 aaaactgcgc ttcgttcgga acggttgcat tcatatatgc tcctgaagat tattcagagg
      181 ttcctcaagg aaaggttttt ctcaaggaaa ggttcctcaa gggactgtct caccggttct
      241 ccgagcattt ccgggaacgg accccctagc tgctgctgtg aagctaatgg aaatgtgaaa
      301 taattgcaag tctgtcacgg cattatctgg aaggtggaaa tggatctcga acaaagccga
      361 tttaacatgg tggagcagca aattcgtcca tgggacgtgc tcgatcagga agtccttaat
      421 ctattgtttg aagtgcggcg cgaggagttt gtccccgctg cctatcggtc tctcgcattc
      481 gtggatatgg aaattcccct cggacatggt gccgtcatgc tggcgcccaa gctcgaggca
      541 cgcatgttgc aggaactgca gataaagaac accgacaaga tcctggagat cggcagtggc
      601 agcgggtatt tcacggctct gctcgccagt aagggcgggt atgtccacag cttggaaatt
      661 gtgccggaat tgaaagcgat ggcggagaga aacctcaaga gacatggaat cggtaatgtg
      721 tcgctggaag tgggtgatgg agcgcgcgga tggccggagc atgcgcctta cgatgccatt
      781 gttttaaccg gttcaacgcc cgttttaccc gaagcattcc aaaaaagcct tcgacccgga
      841 ggccgtctgc tcgcggtggt tggcgatgcg ccggcaatgg aggccatgct ggtcaattgc
      901 atcgctcaag acgaaaaagg aggtttgtat aacagggtca gcctgttcga gaccgtcgtt
      961 tcccctctca aaaacgcgga gcagccggag cgattcgttt tttgaaactt cgggccagtt
     1021 cgattgatag gaaccggacg aatcgcgtct ccatgctcgt gaaacacccc gagacgcttg
     1081 tgcgctggac cggtcattca gcagaaaacc cttaaagtga agctcgcgcg atatattggc
     1141 ccattgttta tcgccggtgt cctgctgcat ggcccatcat atggccgcga cctcatggcg
     1201 gtgtatcgcg aggccctgga gcaggatgcg caatacagat ctgcccatgc cgcatatcag
     1261 gcggcccagg agaagttgcc ccaggggcgg gcggggttgc ttcccacgct cacattttcc
     1321 ggtatcaggc gaaggcagtt gatcgacagg atgggcgccc ccgagcgcac tatcgataac
     1381 cagagcctga cgattactgc cacccagccg atctaccgca aggaaaattt tgtcgtttac
     1441 gagcaggcaa aaatccagct tgcgcaggca gattcgcaat tcatccttgc atcgcaggac
     1501 ctcatactca gggtggcgca ggcttacttc gacattctga cttcccacgt gaacgttgag
     1561 gttgcggaag cgcaaaaaaa ggcgataggg gagcaactgg agcaggcaaa gcggaacttt
     1621 caggtcggta cctccactat tgtggacact tacgaggcgc aagcgcgcta cgatctgacc
     1681 ctctctcagg aaatagccgc ccggaacgac ctggaaatcc ggaagcgcac gctgcagcag
     1741 atcataggca ggatgcctga tgagctcata cggccgaacg agcttacctt cgatttgctc
     1801 accctcaagc atccgaggat ggaggattgg attaccctgg ccgagcagaa taaccttacg
     1861 ctcaagatcc agcaggccat ctataacatt gcgcagcagg atgtggaacg ggccaaggct
     1921 gggcattatc caacgctcga tctggttgcg gtatacagcg accagaaggg agttggcggt
     1981 accattaccg gtcaggggat caacctgact tcgcaagaaa tcgggcttca gctcaacgtg
     2041 ccgatatttc aggggttcgc ggtacagtca cgggtgcgcg aagcgctcgc caaccaggag
     2101 cgggcgcacc aggatctgaa caacacgcgc cgcaacagcg tgttgctggt aagccaacag
     2161 tatctgaacg taaccaacgg catggcgcag gtaaaagcat tgcagcaggc agtagcatcc
     2221 agcaagagtc aactggagtc gacgaggctt ggccgggatg tcggtgtcag aacggaggtc
     2281 gacgtattga atgcgcaaca actgttttat tctgcccggc gcgatctcgc ccaagcacgc
     2341 tataaccttc tcatgtcaag gttgaggctt gaggctgagg cgggggaact ggatgaagac
     2401 gatctgaggc agattaatga agtgttggtg cagtagtttt agaagtaatc gcacaactga
     2461 cgcgagggaa acggggttgt tcaacccggg ctttatcagg ccgagtaacg gttgcaacca
     2521 tgattcgctg gcttaaaccc caaatttagg tcccggattt aaaccccgaa tttaaacccc
     2581 gaatttaaac cccttgtatc tccaggtttt cgccgtttaa gatcatgaac caaccatccg
     2641 aatcccatag ctactgggcg caacggccca ttctctccat agcggatcgg gtggcggcga
     2701 tcggggacac gcgccccaca gacatggata acgctgtact ggacgcaaaa acattcgatg
     2761 cgatcgaagc ggatgagatg ttcgagtcac tcgatcacac cggcacccat ataggcaaat
     2821 gcgtactcta ccggtcactg gcacgcccgg gtaccaatcc tggggtcata cgggaaaaac
     2881 aggaagcggt acgcgagctg gcagcaaacc ctggactccg ggattcgctg cagcgcctta
     2941 tcgatgaggc gggacgtggg gagcaggcgc tttatgactt gctctatgga acattcacgg
     3001 gaggcctggc ggttgataat tccaggatgg gaaaagacgg aatggaattc agcggctatg
     3061 gatacgatca attcatcgac ggcactggct ttgtggttga tgtcgttgaa accgtaaaga
     3121 cattaccgcc gccgcaaagc aagtatctaa agaccttgtt tgatgccatt cgcggttttg
     3181 agcgttcgcg cgtttactcg ctgataagcg gaccagttta tctctcggag ggtaaattca
     3241 agacacgcga agaaaaatcg ggttatatgc ctctcccgcg cttcagaccg tcggttatca
     3301 agcctatccc catgcttttt gccctgggcg cgattagcgt agccttatat gtatttcaga
     3361 ccctgctggc aagctttgga atcgcttacc tgggctacgg aatactcgtg ctgatcgtcc
     3421 cgcttctacc cgtcctgctg atcgcgattg gcgcctccga tcgcgattca gttatctacc
     3481 cgctgcgcaa gctgttcaag cacagtcccg aactggcgca atggcttgat accctgggga
     3541 tgatcgatga gctgaactgt tttcatcgat attcagtggc tttcggggga gatatgacct
     3601 tgccggatat actggagagc gaacggcact ccctgcgcgc atcggaggcc cgcaatccca
     3661 tactgggacg ttcaaatcca acctacgtgc ccaacgacat cctgcttgga gatcccgagc
     3721 ggttgctcgt gattaccgga ccgaacagcg gcggcaagac cgcttattgc aaaactgtcg
     3781 ttcagattca attgctgggt cagatcggct gctatgtgcc tgcggacgcg gcgcaactgg
     3841 ttctggcgga gcacattttc taccaggttc ccgacccggg gcatctggat gagggaatgg
     3901 ggcggttcgg gcatgagctc aaacgcacgc gcgaaatttt cttcaattcc actgcccgta
     3961 gtctcgtcgt gcttgacgaa ctttcggaag ggactacatt tgaagaaaag atggagcttt
     4021 ccgaatacgt cctcatgggg ttccacgagc ttggagccag cacgttactt gtgacccata
     4081 accacgaatt atgcgggcgc cttcaggaaa aagggattgg ccgttacctc cagggcgaat
     4141 ttttagctca agggcccacc tataggctta tcccgggtgt ttcgcgggta agccacgcag
     4201 accgggtagc ggcggcattg ggcttcagca aggaagacgt tgaaaagcac cttgcggagc
     4261 gtaggtcctg atacagcgga agcaattttt accggtccgc gaattcgagt tgattggcac
     4321 gactgcccat cgtatcccgc aaggcggcgt cgggaactgc gagcaacagc gattcgtatg
     4381 actaattcta aagccttctg tagtaacctg tggagggtta ctactctaac gtgatgaaat
     4441 cattagtgac agggggaaac ggttttgtag gttccgcggt ggcgcgccgc ttgctcgatg
     4501 ccggatacga agtgcgctgt cttgtcaggc ccggaagtga ccggagaaac ctccacagcc
     4561 tgcccataga aatcgtggaa ggcgatcttc gttctgttcc ctcattaaaa cgggcggtcg
     4621 ccgggtgcga taacctgttc catgttgccg cggattacag gttatgggtg cccaatccgg
     4681 gaacgatgta tgacatcaat gtaaagggga cagaagcact gatactggcg gcggccgaag
     4741 cgggaatgaa acgaatgatc tacaccagca gtgtggccac cctgggacca agtcgggacg
     4801 gagttccggc taatgaaaac acgccttcga cgctggaatc catgacaggg cactataaac
     4861 gctcgaagtt catggcggag caagtcgtgc agcatatcac ccgggagcac aaattatcga
     4921 ttgtcatcgt aaatccctct acaccggtgg ggccgcgtga tgtcagaccg acgccgacgg
     4981 gacgcatcgt ggtggagaca ctcctcggcc gcatgcctgc ctacgtgaat accggcttga
     5041 atgtcgcgca tacggatgac atagcccatg gtcattttct cgcatacaag catggaaagc
     5101 ctggcgagcg atacatactc gggggcgaaa acatgactct gctgcagata ctacggacga
     5161 tcgacgagat tcaaggcagg cggcgcacgc ggtttaacct ccccgccaac cttgtgttac
     5221 cggccgcctg gctaatggaa aaaattgcgg cggtcaccca tgttgagcca gcggcgacag
     5281 ttgacggcgt tcggatggcg aagaagatga tgttttattc gagcgacaag gctgtgcggg
     5341 agcttggcta ccgatttcgt cccgcagcag aagcgcttcg ggacgcaacg aactggtttc
     5401 gggaaaacgg ttactgcggc taggtttttg gcaagacttc gcagggagat ggatcattct
     5461 actggacgcc cttgcggctc ctgtccacct gaacggcagt aaccaggctg cctcgattac
     5521 cccatgcatt acgaatgtac gataggacca gggcgatctc cttgtcatac aagatctgtg
     5581 agaacggcgg catcccgtaa gggcgtggct cactttccgt aaccggccca taacctccat
     5641 aaagaatgct tcggatcgca ttaacgggcg atgccatcgt cacgccgcgg ttgcccgcaa
     5701 gagcggggta cgcacctgga gcgccttcgc cgaagcgacc atggcagtcc tggcaatagg
     5761 tttcgtatat cttgccacct tcccgcaact gttcgtccac ttcctgggta agttccggag
     5821 ccacccctct cgaatgcggc gccgttcttg gcagcgattg caggtagaca gccatggcgc
     5881 gcgcatcgtc atgcgtcaga tactgaaggc tttggctggt cacatctgcc atgggtcctg
     5941 ccacagctgc ccttgaggac agccctgtcc tgagcagatt caagatctca ggaacctgcc
     6001 agtcggaagt gctggcctcc tggagcgaag tcaaggacgg cgcgtaccag ttggaaccca
     6061 tgatctgccc gccgcccaac gccccatccc tgctccctcc taaaggattg cggctcgtgt
     6121 ggcaggcatt gcaatgcccg agtccctgta tgagataggc accccggttc cactccttgc
     6181 ttttgtcgct tttggctcgg taaatacctt catcgaagta cacgatccgc caaagataga
     6241 tgagcggacg caggttgaat ggaaagcgga cctggttcgg cggattatgt tgctggacgg
     6301 gcggcaggga ttggagatag gcaaaaatcg cgttggagtc ctcgcgcgtg attttcgtgt
     6361 attcggtata aggaaacgcg ggatatagcg cgctgccatc cctgcgcttg ccctcatgca
     6421 acgcgcgcca gaaatcgtct tcgctccaga ggccaattcc ggtttcccga tccggcgtga
     6481 tgttgggcgt aacgaatgtt ccgatcggtg tatcgaggtt gtggcccccc gcataaggct
     6541 ggccgggctg cgccgtatgg catcccatgc aattaccgat ttttgcgagg taggcgcctc
     6601 gtgcttgctg ccacttccgt tgagcggcaa catcttctgc cgcgtccatt gctactggca
     6661 atccttcatt cgccgccggt aggctgatcg atacattgat acccaaaccg gtaagtccgg
     6721 ccagtgcggc aactgcgata attcggttca tttctgctgg gtcgccggcc gaataatggt
     6781 gctgcagcgc ttggcaagtt tttgggggag tgttgctgca ggagaccctg gcctgggcgc
     6841 tatttctgaa acgggctggg cagccagcca tttcgctacg ccaacggctt cttcgggagc
     6901 gagctgtttt gcgatttccg acatacagtc gggtgtctgg ccacgtatca agccgccgtt
     6961 ctgccagttg ccgaattgag caatgatgta aacacgcggc agacccagca atccgggaat
     7021 gaaaggagcg gtccccatca aatccttgcc atggcattct acgcaccccg gaatatttcg
     7081 cgcggcatcg ccctcataga taagcctgcg aacgcgttca gcctccgcgg aggataaagt
     7141 catcggctct ggcggaggaa acgcctgttc cagcgaggcg aagtagacgg ccatctcctt
     7201 caggtattga tcgggtaaac ctttcagcag ccaagccatg ggctgatagt accgtcggcc
     7261 atccctgaaa ttgcgcagct ggttgaaaag gtagccttcg ggtttgcccg cgatccgggg
     7321 ataataggca tcccttcctt ccttatcctc gggtccatgg caggcgacgc acgctttcac
     7381 ccgctctacc atcgtgttgg gaaccttgtt cccgggaagc ttggccggtg gaattgccgt
     7441 tgccgttgcc gaaaggttaa aaaacaggca agtgatcagc atgatcaaga gaaaggatct
     7501 catctgcgta cgtctttgtt ccctgtcagt tttactcaaa ccgttttact caaaccctct
     7561 gcgggctggt taaaatggaa ggcctggagc ttccatcacc gcgcaaattt ccttaccacc
     7621 gccatgagct cgtcaattgc ctcgtcaccc ttgccggact tgattgcgcc ctgcatgcag
     7681 ccgtgggtat ggctttccag cagcagcatg gcaactgcat cgagcgcgga ttgcaatgcg
     7741 gctacctggt tgaggatgtc aacgcaatag cgatcttccg atatcatctt gttgacgcca
     7801 cgcacctggc cctcgatccg gttgaggcgc ttgatcaaag cctctttgtc cggttgcacc
     7861 accgtgcgtg gcgcatggca cgattccttc ttattttgcg acttcaaaac cggcaccctc
     7921 gattgcgttc ttgaactgct ccgcgccggt tttgccagcg tcatactgga tcttcacctg
     7981 tttctgttcg agggaaacgt ctgcgttttg aacgcccggt attttctcca ggacgttctt
     8041 cacgctgttg acgcaccctt gacaggtcat tccatttacc tttatgactt ctgtttgcat
     8101 ttcgcgactc ctttcagtca gttgatttga tgaaccggtc acgcgccagc ccgccatcgt
     8161 ttcagcaaca gtgaattact gaccacggat actgaactca ttgccattgc cgcgcctgca
     8221 atgacaggat tcagcaatcc catcgccgca agcggtatgc ccaatacgtt gtagatgaag
     8281 gcgaagaaca gattctggcg aatttttctc aatgtcgcgc gggaaagcga aattgcatcc
     8341 gccacgctca tcaagtcatt tcgcatcagc gttatatcgg ccgcttcaat cgccacatca
     8401 gagccggctc cgatagcaaa actgacatcc gccatagcca gggctggagc atcattaatg
     8461 ccgtctccca ccattccgac gcatcgcccg cctgctttca tttccctgac ttgcgcggct
     8521 ttttcctgtg gcaacacttc tgccctatag tctggaatac ccgcttgcct ggcaatggcc
     8581 gcggcagtgg cgggattatc tccagtcagc attacaactt cgatatccat tgcccgcagg
     8641 cgatggacgg cagcggcgga tgtggcgcgc aggcgatccg cgatggcaac atatcctaat
     8701 atccgcggcg aattgccggg cagggcggat agggccacaa cggtttttcc gtccgcctga
     8761 agcgcgtcta tcaggcgttg atcgaccaca gctcctttct ccgtcagaaa tttgggcgaa
     8821 ccaagaaggt attcaacgtt atcgataact gcgctgattc cactgccggt aatagcggta
     8881 aaacctttca tcgtccccgg ttgaacgccc gcgcgagcag cgtactccac caccgctctg
     8941 gccaaagggt gttcagaccc ttgctccagc gtagctgcgg tctggatgag gtcgtgttcg
     9001 gcagtgccgc caacgggaat aacgtctgtt accgtaggtt ccccctcggt cagcgtgccc
     9061 gttttatcca caatcaaggt ccgaattttt tcggcatgct ccagggcggc agcattcttc
     9121 accagcacgc cggcttgcgc accctgtccc attcctacca ttatcgcggt tggcgtcgct
     9181 aacccgagcg cacagggaca ggcaatgacg agcactgcca cggcgttgac cagcgccgga
     9241 acaaagccgc ctgtgagcca ccatgtgcct gcaaaggtgg ctgcgctgac aatcaccacg
     9301 ataggcacaa atacgccgga aatcgtatcc gccatcctct ggatgggcgc cttggaacct
     9361 tgcgcttctt cgaccagccg aacgatggct gcgagctgcg tatgagatcc cacgctcgtg
     9421 gcgcggcact tgagcatacc ctgccgattc acggtggcgg cataaa
//
LOCUS       DASSHJ010000071        11041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_858819, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000071 DASSHJ010000000
VERSION     DASSHJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858819"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFI43_02885"
     CDS             complement(<1..899)
                     /locus_tag="VFI43_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7061104.1"
                     /translation="MSKTKEHIREATSAARVMAAQAFSRAAGAPLVHGNSIRLLKDAA
                     ENYPAWFEAIRSAEKTVYFENYIIRDDKIGQEFSDVFISKAKEGVRVRVLYDWMGALT
                     ETSSSFWRRLAENGVEVRCFNPPRLDSPLGWLSRLHRKSLCVDNRVAFVSGLCVGQMW
                     VGYPERGIPPWRDTGVEVRGPIVADVARSFSLVWAEAGPELPVDELPSQASIPPVGDV
                     DLRVLGSATNTAALYRLEHLITVLAQKTLWLTDAYFVGTTSYVQALRGAAMDGVDVRL
                     LVPGSSGDLKFLRPISRSGYRPL"
     gene            1367..1549
                     /locus_tag="VFI43_02890"
     CDS             1367..1549
                     /locus_tag="VFI43_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061105.1"
                     /translation="MKARFFVYLGMLLLVTPALSGCWLLAAGGVGAYGGYKAKEEGYE
                     VRSPVTKKPQTEDTKD"
     gene            complement(1608..4919)
                     /locus_tag="VFI43_02895"
     CDS             complement(1608..4919)
                     /locus_tag="VFI43_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7061106.1"
                     /translation="MSRPPTWLIILLYAASSVLWVAVAGYIISLTLDDPVLRSRAYLA
                     NGLILVGISTALFYLLLKIGKDTESAPIIHPPRPARLNRVLLMLVPLAMVAPLVSIGI
                     VNMYAPELERGAYADLQTIGDLKAEQIELWLDERHGDAETLAVSHTLIERVAHWKREK
                     DERVRRLVVNRLDAVRKAYSYESVLLQDPDGETLLSLGEQRKLPSITEALRSTVLQTN
                     RIQSTDLFLDEAGKPRLDILVPLTPKPTGEQPVAAIILRANLELFLIPLIEKWPGNSA
                     SAEILLIRQTGNTESHLNNLRNPDKTGTGNSDSRSIVSTNITTSLAGGERGTAQGTDY
                     RGETVFAAYRPIAGSEWRLLAKIDRAEVFAQLWTLVFWVTVVILAAIAAVSMILLLLW
                     RQQREAQQLAFMVHTAEQDKLLKYFYELPFIGMAITSPDKRWLRFNDQLCSILGYSRE
                     ELEKKTWAETTHPDDVAKDISEYERVIRGESEGYAMDKRYIRKDGSVVLANLEVKCVR
                     KSDGRVNYFIAMIRDITEQRRQQNELLAARSQLQATLDAIPDLLFELDPQAYCLAWHS
                     ARTSLAALQVEDLVGRRISDIFSPDTADVIASALQEAQDKGFSSGKQVKLELPFGILW
                     FELSVSRKQVAPGSEPRFIMLARDVTERKASEQKIKNLAHFDVLTGLPNRALLADRLK
                     VAIRRAARQSGRLAVLFVDLDRFKPINDSLGHDVGDKVLKVVAERMQDSVRSEDTVSR
                     VGGDEFVVLLTEIGSAEDVARVAEKLIAELSRPYKIEEHDLALGASIGICIYPDNGSE
                     PGVLLRNADASMYSAKQAGRNGYQFYSEEMTSRAIERLSLERDLRVASPQKEMFLVYQ
                     PQIELATGSVVGVEVLLRWRHPVHGLISPLRFIPVAEDTGLILSIGEWILRESCQHAK
                     RWLRRGLLDACICVNVSAVQFRQIDFVQLIRDALEDTRLPPDRLELELTERVLMQTVE
                     PSLEKLHELKTLGVKVAIDGFGTGYSNLSCLRQFMIGRLKIDQSFVRRLPGNGDAESV
                     TAAIIAIGERLSLRTVAEGVETQEQAECLEGMSCKEAQGYLFAAPMARAEFEAWIEGR
                     RRAPGKTGGSPRGNA"
     gene            5167..6006
                     /locus_tag="VFI43_02900"
     CDS             5167..6006
                     /locus_tag="VFI43_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061107.1"
                     /translation="MKTRFIIFALPIFVVAATASAAEEACRNVLSRVFYNQYSTIEPR
                     TRDRAVYAELCASEYPQAQQIVKRARQSGDDGSLGLWYGLFNLDDIEPGGKGTSALSL
                     SEDRFRQWKAGYCSKTSEVEAYQAAGLFMQSAMTAKGGQNKGLDGWSSCMRKQEGLAC
                     WATPGLQQSNVVLNVHWMAPSSTDAASHAQPEVQYSFVTRGIVSKFDGAPAKRLLPDG
                     YKLKPGTTQIPVTREADTTGTANLKVNFGGREHNCKVFIPGDRDFTMSEPFVNRLKIK
                     YPG"
     gene            6211..7170
                     /locus_tag="VFI43_02905"
     CDS             6211..7170
                     /locus_tag="VFI43_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HET7061108.1"
                     /translation="MDHTEHPAGHDHPQTFDEVIAEGFPHDKLKQLAEAHEKRISENP
                     PAHAPKLFARRAFAAAPVVKVTREDQAALSPEKRSAFRDAIVRLVEEGKYLEIVGNHM
                     DMSHMMHGTMGEVGLYRFLPWHRRYLLELERELRRVDAELRPEATEKIGIPYWRWEDP
                     FPEWLSDFLPANDPDTGDAPAPRRNAAPPPKPNAADIDIIVNQFSIQRTGLAGENDYT
                     KFTYGIEGWGRRPDGSPLPAHNNGHAWVGGIMNNTRTSPTDPVFWLHHAEIDRLWQVW
                     RDSHPAPEPSLSGTDRIMDPWGESYNDLLDIDALGYTYDSVSL"
     gene            complement(7206..8711)
                     /locus_tag="VFI43_02910"
     CDS             complement(7206..8711)
                     /locus_tag="VFI43_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061109.1"
                     /translation="MSPDGSKTTWKPAAREAGIRVRMYRVGFGDFFLVTFLDRNGKPL
                     HVIVDCGVFRGKDQTGDLHSIEAAVSDMAQTTEGHVALVVITHRHADHIAGFARCEDA
                     FRKLSVDAIWMPVWESEYEPVALKYQMELTRTALALEQHFAALGADATEADAVARAYM
                     ENAIGEVPTGASAVAGKGTNAKALELLKHGFADTKPQYYKSGDKPELPQALVDAGLEC
                     EILGPPPVTALELMKLMNLQKNVGQYLAAVGQASGSTDDLKPFGSEWEIDPALEPEKS
                     EREFYTPESFREWNEERKSWGDITGEEATRARLKMEANLQQLQPAAALTAAKQLNTFL
                     NNQSLVVLFKFKGKKLLFVGDAQAGNWEHWLFDTDVPDKKAGGELSTDARQILTSLDF
                     YKVGHHGSGNATPKVAAETMGKQQKFISMCSTQADVYGNEDPDNPAKGTEVPRLPLLE
                     KLASESALVRSDQISITVNEKEIEAKVAAPLPAPPPGSRLEKGTLWVDCYL"
     gene            complement(8714..10543)
                     /locus_tag="VFI43_02915"
     CDS             complement(8714..10543)
                     /locus_tag="VFI43_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061110.1"
                     /translation="MNNKPGSTRLLAPRRRPLRVFAFDPSHGRLLGNEMQVDVRYRGL
                     APGPVDRSGAHDQIAVVDYDISRKKYYRPVDLDDPFILISNGIAPSEADPRFHQQMVY
                     AVASDTIEQFENALGRRIHWRRAERPLDSPPGWLPDDILTLTLFPHAMQQANAYYSPE
                     AHGILFGYFKAGLENAGRNIPGQTVFTCLSHDIIVHELTHAIVDGMRSHFTEQTNPDV
                     AAFHEAFADLAALFRHFTHREVLLDAIQRTGGRLYTSAMNGDMLADLAAQSWLTVGEA
                     ERNPLVELASQYGEATGKNQGLRSAIGKPKTMAELRGTTESHERGSIMVAAVFDAFFT
                     VYLQRAAKHFQIYRSGGGNESANLPSPLAEACCEEAVATAEYFFRTCVRALDYLPPVD
                     VTFGDFLRAVMTAESDYDPEDPEGIRSAWMQAFRHRGILPDDALSFSEAALCWPPCPH
                     DIKVESLPFGGPLGPSYEERKQIQEALKKFMDKAENKKILDLDADMGYAIPSFHTSYR
                     MNRSGNVRWDMVVEVVQTATGESGTYPRRGGTTMIISSGSTGGGGNKDIVFLRYAISK
                     PLGGDKGLIRAERQQSFLAEQGIKPGGSASDLRINFALLHSED"
     gene            10976..11041
                     /locus_tag="VFI43_02920"
     CDS             10976..11041
                     /locus_tag="VFI43_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061111.1"
                     /translation="MLAGLALLGWTVRRKNSLREG"
BASE COUNT         2399 a         3094 c         3033 g         2515 t
ORIGIN      
        1 aacagtgggc gataaccgga acgggaaata ggtctcagga atttgagatc cccgcttgaa
       61 cccgggacga gcagccggac gtcgacgccg tccattgcag cgcctctcaa ggcctgaaca
      121 tagctggttg ttccaacaaa ataggcatcc gtgagccaca gtgttttctg ggccagcacg
      181 gtgatcaggt gctcgagccg atacagcgcc gcggtgttcg tcgcgctacc taatacgcgc
      241 agatcgacat cacctacggg agggatggaa gcctgggatg gtagttcatc gacgggtagc
      301 tcgggacctg cctctgccca gaccagggaa aacgagcggg cgacatcggc gacaataggc
      361 ccgcgtacct ccactccggt gtcgcgccag ggagggatgc cgcgttcggg atatcccacc
      421 cacatttgcc cgacacacaa tccgctcaca aatgcgacac gattatcgac gcagagggat
      481 tttcgatgca gcctgctcaa ccatcccagc ggactgtcca ggcgaggcgg gttgaaacag
      541 cgcacttcaa cgccgttttc cgccaagcgg cgccagaagc tgctcgatgt ttcggtgagt
      601 gcccccatcc agtcatacag cactcggacg cggacacctt ccttcgcctt ggatatgaaa
      661 acatcggaaa actcttgtcc gatcttgtca tcgcggatga tatagttttc gaagtaaacc
      721 gttttttccg cggagcggat tgcttcaaac caagccgggt aattctcagc tgcatccttg
      781 agaagccgaa tactgtttcc atgcacaagc ggcgcgccag ccgcgcgcga gaacgcctgc
      841 gccgccatga cgcgcgctgc gcttgttgct tcgcgaatgt gttcttttgt ttttgacatt
      901 ctattgtccg gggcgggaac ggttccggct gcctcacgag tacgggacga gtggggtgcg
      961 ggacagtttt attgctcccg ccaacgcaag tgacgactcg ccagcactgt aaactggcaa
     1021 tccaggctgt gaaaaaacct gagaaaacag cacctcaatc gaaaatattc ccacttatat
     1081 gctcactact cgccattaat aggttctgca ttccactcag atttcggcta tattcgcaaa
     1141 actcacttgc cttcccacct cggaaaatcc ttcgagagtt tttcacagct tgtttcgcgg
     1201 ggctgactta aacccctcga tttttacgca tgaactcgag gaagtgtttg taaccgtaca
     1261 gatgaataat ggccctcgcc ttatattaac tgtaggcaat aggcaggagt gtagcaaaaa
     1321 gagttcccga tccagggttt gcggcataaa tttgagggag acgttaatga aggcgagatt
     1381 ttttgtttat ctgggaatgc tgttgcttgt gactccggcc ctgtcgggtt gctggttgct
     1441 tgcggcaggc ggtgtagggg catacggggg atataaggcg aaggaagagg gatacgaggt
     1501 tcgatcgccc gttacaaaga aaccccagac cgaggacacc aaggactaag tatctatcat
     1561 tgcatgcgcg gctgggaagg ggcattttca caggttactg aggcctttca agcgtttccc
     1621 cgcggactgc cacccgtctt ccctggcgcg cgacgcctgc cctcgatcca cgcttcaaat
     1681 tccgctcggg ccatgggtgc ggcaaaaaga tatccctggg cttccttgca cgacataccc
     1741 tccaggcatt cggcctgttc ctgcgtttcg acaccttcgg caacggtacg caagctgagc
     1801 cgctctccaa tcgctattat cgcagcggta acggattcgg catcgccgtt cccaggcagt
     1861 ctccgcacaa atgactgatc aattttcagc cggccaatca tgaattgacg caggcaggag
     1921 agattcgaat atcctgttcc aaaaccgtcg attgctacct ttaccccaag cgttttcagt
     1981 tcatgaagtt tttcaaggga aggttcaacc gtttgcatga ggacgcgttc ggtcagttcc
     2041 agctcaagcc tgtcaggtgg gagacgggta tcctccaacg catcccggat gagttgcacg
     2101 aagtcaatct ggcgaaattg cacggctgaa acattcacgc agatgcatgc atcgagcaaa
     2161 cctcgccgga gccagcgttt ggcatgctga caggactcgc gcaggatcca ctcaccaatc
     2221 gacaggatga gaccggtatc ctccgccacg ggaataaagc gaagtggtga aatcaatcca
     2281 tggacaggat gccgccagcg aagcaatact tcaacgccca cgacactgcc tgtcgccaac
     2341 tcgatctgcg gctggtacac caggaacatt tctttctggg gcgaggcgac ccgaagatct
     2401 cgctccaggc ttaaacgttc aattgcccgt gacgtcattt cttccgagta aaactggtat
     2461 ccgttgcgtc cggcctgttt ggcgctgtac atggaagcat cggcattccg gagcagaaca
     2521 cctggttccg agccattatc cggataaata cagataccga tacttgcgcc gagggccagg
     2581 tcatgctcct cgattttgta aggacgggat agctcggcga tgagcttttc ggccacgcgc
     2641 gccacatcct cggcgcttcc aatttccgtc agaagaacaa cgaattcgtc tccgccaacc
     2701 ctgcttaccg tatcttcgct ccgaacagag tcctgcatgc gctctgccac aaccttcagc
     2761 accttgtctc ccacatcgtg gcccagtgaa tcattgatgg gcttgaagcg gtcgagatcg
     2821 acgaacaata cggcaagccg gccggactga cgcgctgcac gtctgattgc aactttcaat
     2881 cggtctgcga gtaacgcacg attgggaagc cccgtcagaa catcgaagtg ggccaaattc
     2941 ttgatctttt gctccgacgc cttgcgctcg gttacatcgc gtgcaagcat gatgaaccgc
     3001 ggctcggatc ccggagcgac ctgtttgcgg gaaaccgaga gctcgaacca gaggataccg
     3061 aaagggagct ccagctttac ctgttttcct gacgagaaac ccttgtcctg tgcctcctgc
     3121 aatgcagagg caataacatc cgcagtatcg ggagaaaaaa tatctgagat cctcctgccg
     3181 acaagatctt caacctgtaa agcagccaga gatgtgcggg ccgaatgcca ggcgaggcaa
     3241 taggcctgag gatcgagttc gaacagcagg tcgggtatcg catccaatgt ggcttgcagt
     3301 tggctacggg cggcgagaag ttcgttttgc tgccgtcgtt gctcagtaat gtcccgaatc
     3361 attgcgatga agtagttgac ccttccgtcg ctcttgcgga cgcatttgac ttcgagattc
     3421 gccaggacta cggaaccgtc cttgcgaatg taacgcttat ccatggcgta accttcagat
     3481 tctccacgga tgacccgctc gtattcggaa atatccttcg caacgtcatc tggatgagtt
     3541 gtttcagccc aggttttttt ttcaagctct tcacgagaat aaccaaggat ggagcagagc
     3601 tggtcgttaa accgcagcca gcgcttgtcc ggtgaggtga tcgccatacc gatgaacggt
     3661 aattcataaa aatatttaag gagcttgtcc tgctccgcag tatgaaccat gaaggccaat
     3721 tgctgcgcct cgcgctgctg tcgccacagg agcagaagaa tcatgctgac agctgcgata
     3781 gctgccagga ttaccacagt gacccagaaa accagggtcc aaagttgcgc gaagacttcg
     3841 gcgcggtcta ttttcgcgag caggcgccat tcgctacccg ctatgggtcg ataggcggca
     3901 aagaccgtct cgccccggta gtctgttcct tgtgcggttc ctcgctctcc cccggcaagg
     3961 gacgtggtga tattcgtgct gacgattgac ctgctgtccg agttgccggt acccgttttg
     4021 tcggggttgc gtaaattatt gaggtggctt tcggtgtttc ctgtctgccg tatcagcaga
     4081 atctccgcgc tcgcgctatt gcccggccat ttctcgatga gcggtatcag gaaaagctcc
     4141 aggttcgcgc gtaaaatgat ggctgcgacc ggttgttcgc ctgtgggttt cggcgtcagc
     4201 gggaccagga tatccaggcg aggctttcca gcctcatcca gaaacagatc agtgctctgt
     4261 atgcggttcg tttgcaggac ggtagagcgc aatgcctcgg tgatggaagg aagcttgcgc
     4321 tgttctccca ggctcagcaa agtctcaccg tctgggtcct gtagcagcac ggattcataa
     4381 ctgtacgcct ttcgaaccgc atccagccga ttaacgacaa gccggcggac ccgctcatcc
     4441 ttttcgcgct tccagtgagc cacccgctcg atgagcgtat ggctcacggc cagcgtctct
     4501 gcgtcgccat gtctttcgtc cagccaaagc tcgatttgct ccgccttgag gtcaccgatg
     4561 gtttgcaggt cggcataggc tccacgttcg agctcgggcg catacatgtt cacgattccg
     4621 atgctcacca gaggggcgac catcgcgagc ggcaccagca tgagcaacac tcggtttagc
     4681 ctggcaggac ggggagggtg aataatcggc gcagattcgg tgtctttgcc tattttgagc
     4741 aggagataga agagcgcggt gcttatcccg accaggataa gtccattcgc aaggtatgcg
     4801 cggctgcgca acacggggtc atccagggtg agggaaataa tgtagccagc aaccgctacc
     4861 cacaggacgg aagaggctgc atagagcaaa atgatgagcc acgttggggg gcggctcatc
     4921 agttaacggt tgctcctgga aaacctatta ctgcgaggga aagttgcatt gctgaaaata
     4981 agcctcccgg tttagtgata ggtgccagcg agcgagccag agactatctt ggactgttac
     5041 gctaacatat gcatgttctg gctttccgtc tgtatgctcg cgaacaatgg cggcggtaat
     5101 ggcggaaaca atgtcagcag gaatgcaatc ccggtcacta tattattgat ggattattcg
     5161 aacattatga aaactcggtt tattattttt gccttgccga tatttgttgt ggcggcaacg
     5221 gcgtccgcag ccgaggaggc gtgccggaac gtcctttctc gcgtttttta caatcaatac
     5281 tcgaccatcg aacctcgcac gagggatcgg gcggtgtatg ctgaattgtg cgcatccgag
     5341 tatccccagg cgcaacagat cgtaaaacgt gcccgccagt caggcgacga tggttccctc
     5401 ggtctttggt atgggctatt caacctggat gatatcgagc cgggcgggaa gggcacgtct
     5461 gcgctttctc tcagcgaaga ccggttcagg cagtggaaag cgggatattg ctcgaagacc
     5521 tctgaagtcg aggcctatca ggcggccggg ctgttcatgc aaagcgcgat gacggcgaag
     5581 ggagggcaga ataaaggcct tgatggctgg tcgtcgtgca tgcggaaaca agaggggctg
     5641 gcctgttggg ctaccccggg cctacagcag agcaatgtcg tgctgaatgt ccattggatg
     5701 gcaccatcct ccacggatgc tgcgtcccat gcgcagccgg aagtccaata ctccttcgtg
     5761 acgcgaggga ttgtttccaa atttgacggc gcgcctgcga agaggctgct cccggatgga
     5821 tataaattga aacctggaac gacacagatc cctgtcacgc gggaggcgga tacaacaggc
     5881 actgcgaacc tcaaggtgaa ttttggcggc cgtgagcaca actgcaaagt tttcattcct
     5941 ggcgatcggg attttacgat gtctgagcct ttcgttaacc ggttgaagat caagtatccc
     6001 ggctgagtcc agccttaatc atattcaagg aaatctcgcg catttcccca tgcgaatccc
     6061 cgcagttccc atccagcccg accctcgggt ttggcttggg aaacgataaa tttaaccaaa
     6121 attttaccgt tccattacga cttcgttttt tgggtcgcgt atcgttattt ttggctatga
     6181 tgtcaacgtt gaagacaaca aggagaaaga atggatcata cagaacaccc tgccgggcat
     6241 gatcacccgc aaacatttga tgaggtgatc gccgaaggtt ttccgcatga caagctgaaa
     6301 caacttgcgg aggctcatga gaaaaggatc agtgaaaatc cccctgctca cgcgcccaag
     6361 ctgtttgcaa gacgtgcctt tgctgctgcg cccgtcgtca aggtcacccg ggaggaccaa
     6421 gccgcgttga gcccggaaaa gcgctccgct ttccgcgatg ccatcgttcg gctcgtggag
     6481 gaaggcaaat acctcgagat tgttggcaat cacatggata tgtctcacat gatgcatgga
     6541 acaatggggg aggttggtct ctatcgtttt ctgccctggc accgccgcta cctgctggaa
     6601 cttgaacgcg aactccgccg cgtggatgcc gagttgcggc ctgaagcaac tgaaaagatc
     6661 ggaattcctt actggcgctg ggaagaccct ttccccgaat ggttgagcga tttccttccg
     6721 gccaacgacc ccgataccgg agacgccccc gcccctcgga ggaacgcggc ccctcctccc
     6781 aagcccaatg ctgccgacat tgacatcatc gtcaaccagt tttctatcca gcgaacgggg
     6841 ctggctggcg aaaacgatta caccaaattt acttatggaa tagaaggctg gggtcgccgt
     6901 ccggacggca gccctctgcc ggcgcataat aatgggcatg cctgggtcgg cggcatcatg
     6961 aataatacgc gcacctcgcc cacggacccc gtgttctggc tacatcatgc ggagattgac
     7021 cggctgtggc aggtgtggcg agacagccat cccgcgcccg agccctcgct aagcggcacc
     7081 gaccggatca tggatccttg gggagaatcc tacaatgatc ttctcgacat cgatgccttg
     7141 ggctacacct atgattcggt gtcgctctag cggaaagtat ttctcgtgat gccgcgcttt
     7201 gttcgtcaaa gataacaatc aacccagagc gtgccttttt ccaatcggct gccgggcgga
     7261 ggcgccggca ggggcgcggc aaccttagcc tcgatctctt tctcgttaac ggtgattgaa
     7321 atctggtcgc tgcgaacgag cgcgctttcg gaagcaagtt tctccagaag aggaaggcga
     7381 ggcacctccg ttcctttcgc gggattgtcc gggtcttcgt ttccgtagac gtcggcttgt
     7441 gtggaacaca ttgatatgaa cttttgctgt ttccccatcg tttccgcagc tacttttggc
     7501 gtcgcgttgc cactgccatg gtggcccact ttgtagaaat ccaggctggt cagtatttga
     7561 cgcgcgtctg tcgatagctc gcccccggcc ttcttatccg gcacatcggt atcgaatagc
     7621 cagtgctccc agttgccagc ctgcgcatca ccgacgaaca agagcttttt gcccttgaat
     7681 ttaaacagca cgacgaggct ttgattattg aggaacgtgt tgagctgttt tgccgccgtc
     7741 agggctgcag cgggttgcaa ctgctgaaga ttcgcttcca ttttcagcct cgcccgtgtg
     7801 gcttcctcgc ccgttatgtc cccccaggat ttgcgctctt cattccattc ccggaatgac
     7861 tccggggtat aaaattcgcg ctccgatttc tccggttcaa gcgccggatc gatttcccat
     7921 tccgagccga aaggtttcaa gtcatcggtg gagcctgacg cctggccgac tgcggcaaga
     7981 tactgcccga cgtttttctg aagattcatc aacttcatca gttccagcgc agtgacagga
     8041 ggaggcccca atatctcgca ctcaaggcct gcatcgacga gcgcttgggg gagttccggt
     8101 ttatcgcccg acttataata ctgaggcttg gtatcggcaa atccatgttt gagaagctcg
     8161 agggctttgg cattcgtccc ctttccagcc actgccgagg cgcccgtcgg aacctcgccg
     8221 attgcattct ccatgtacgc ccgagctacg gcgtcagctt ccgtcgcatc ggcgccgagc
     8281 gcggcgaaat gctgttccag ggcaagagcc gtccgcgtca gttccatttg gtatttcagc
     8341 gcaaccggct cgtattcaga ttcccagaca ggcatccaga tcgcatccac tgacagcttc
     8401 ctgaaagcat cttcacaccg ggcgaatccg gcaatatgat cggcgtgccg atgcgtgatc
     8461 acgacaaggg ctacgtggcc ttctgtcgtc tgagccatgt cggaaaccgc cgcctcgatg
     8521 ctatgcaggt cccccgtctg atcttttccc ctgaagacgc cgcagtccac gatcacgtga
     8581 aggggctttc cattgcggtc gaggaaggta accaaaaaaa agtctccaaa acctacccgg
     8641 tacattctta ccctaattcc cgcttctcgg gcagcgggtt tccatgtggt cttactcccg
     8701 tctgggctca tattcagtcc tctgaatgca ggagcgcgaa gttgatgcgc aggtcgctgg
     8761 ccgatccgcc cggcttgatt ccctgttcgg ccaggaagga ctgttgcctt tcggcacgaa
     8821 tcaaaccttt atccccgcct aagggtttgg aaatcgcata gcgcagaaat acgatatcct
     8881 tattgccacc gccccccgtg ctgcccgacg aaatgatcat cgtggttcca ccgcgccgag
     8941 ggtatgtccc cgattcgccg gtcgccgtct ggacaacctc aaccaccata tcccagcgca
     9001 cgttaccgct gcggttcatc cggtacgaag tatggaacga gggaatggca tatcccatat
     9061 ccgcatccag gtcgaggatc ttcttgtttt ccgccttatc catgaatttt ttcagcgcct
     9121 cctgtatctg cttgcgttcc tcgtagctcg gccctaacgg gcctccgaag ggaagcgact
     9181 ccactttgat gtcgtgagga catggtggcc agcagagcgc tgcctctgaa aaggagaggg
     9241 cgtcgtccgg caggatgccc cggtggcgga atgcttgcat ccaggcgctt cgtattcctt
     9301 cagggtcttc gggatcgtag tcgctttccg ctgtcattac agcccgcagg aaatccccga
     9361 acgtaacatc caccgggggc aaataatcca gggcgcggac acaggtgcgg aaaaaatatt
     9421 cggctgtcgc gaccgcctct tcgcagcaag cctcggccag aggggaggga aggttcgcgc
     9481 tctcattgcc accgcccgaa cggtagatct ggaagtgttt tgctgcacgc tgcaagtaga
     9541 cagtaaagaa ggcgtcaaat accgccgcca ccattatcga gcctcgctcg tggctctcgg
     9601 ttgtgccgcg caattccgcc attgtcttcg gcttgccgat tgccgagcgc aacccctggt
     9661 ttttgcccgt cgcttcgccg tattggcttg ccagctcgac cagcggattg cgctctgcct
     9721 cgcccacggt tagccatgac tgcgccgcca gatcggcaag catatccccg ttcatcgctg
     9781 aggtgtacaa gcgtccaccg gtacgctgaa ttgcgtcgag cagaacttcc cggtgcgtaa
     9841 aatgccggaa aagggccgcc agatcggcaa aagcctcgtg aaaggctgca acatccggat
     9901 ttgtctgctc ggtaaagtgg ctgcgcatgc catccacgat ggcatgtgtg agctcatgca
     9961 caataatgtc atgagagagg caggtaaaaa ccgtttgacc aggaatgttt cgtccagcgt
    10021 tctctagtcc tgccttgaaa taaccgaaca ggatgccatg cgcttccgga gaatagtacg
    10081 catttgcctg ttgcatcgcg tgcggaaaca gggtcaatgt aagaatgtcg tcagggagcc
    10141 agccaggtgg gctgtcgagc gggcgttcgg cgcgtctcca gtgaattcgc cgcccgagcg
    10201 cgttctcgaa ttgctcgatg gtatcgctcg ctaccgcgta gaccatctgc tgatgaaagc
    10261 gcgggtccgc ttccgagggc gcaattccgt tggaaatgag tataaacggg tcgtccagat
    10321 cgacgggccg gtaatatttt tttcgggaga tgtcgtagtc cacaaccgcg atctggtcgt
    10381 gtgctccgct tcggtccacc ggtccgggag ccagaccgcg gtaacgcacg tcaacctgca
    10441 tctcgttgcc gagcagccgg ccatggctcg ggtcgaaggc aaaaacccgc aagggacggc
    10501 gcctcggggc aagcagtcgg gtcgagccag gtttgttgtt catcgtcctc ccctttaaaa
    10561 cccgaaaaag ctaaaaggct tcgaaaaccg cttctgtcgt tctaacgttg tgttcatgct
    10621 gccttttcag ctctcttcag ttgaccactg tatcaccttg ggtgtagcag atagcttcag
    10681 aagatcaagc cagagaaatt aagggatgcc ggagtcaacc gtcccggttt ttagaaggct
    10741 ccaacatcgg agaatggagc catgataaat caaacaaagt ttccccctaa gtgcgtgagg
    10801 gtgcggtacg gatggtattc gaatgccggg cgagcatgcc ttcatgtagg caaccaatga
    10861 atacattgcc caagatcggt tgggtcctcc agaccccgaa tgatctggtc cgactgtcgt
    10921 gcggatcaaa ttgctcgatc gagcgcctat tccagaacct gagtcttatg ccctaatgct
    10981 tgccggtttg gcccttctag gatggacggt acggaggaag aattcgctgc gcgaaggttg
    11041 a
//
LOCUS       DASSHJ010000072         7709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_631316, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000072 DASSHJ010000000
VERSION     DASSHJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631316"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..746
                     /gene="plsY"
                     /locus_tag="VFI43_02925"
     CDS             138..746
                     /gene="plsY"
                     /locus_tag="VFI43_02925"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7061112.1"
                     /translation="MMPLVFILLAYLLGSVSFGVLASWIFDLPDPRTYGSRNPGATNV
                     LRSGRKAPAVFTLLGDAGKGWLAVAAAQYFAPILGFGSWTVALVALAVFLGHLFPIFL
                     GFQGGKGVATALGVLLGLNPWMGLLAAAIWIAVAATWRISSLAALTAAVLAPIYAVFF
                     LGFEARTAAVVVMSLLLIWRHKSNIAGLIAGTETRIGKRGTS"
     gene            complement(776..1837)
                     /gene="tsaD"
                     /locus_tag="VFI43_02930"
     CDS             complement(776..1837)
                     /gene="tsaD"
                     /locus_tag="VFI43_02930"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7061113.1"
                     /translation="MLVLGVETSCDETGVALYDSDRGLLSHALYSQTEMHSEYGGVVP
                     ELASRDHIRRVLPLIRQTLASVEADLKNLDAIAYTQGPGLAGALLVGGSIAAALGFAL
                     NIPVLGVHHLEGHLLSPLLSTPAPAFPFIALLVSGGHTQLMEVSGLGQYKLLGETLDD
                     AAGEAFDKTAKLLGLGYPGGPALSRLAEEHLQSGAPRYFHLPRPMLRSGDLDFSFSGL
                     KTAVSTLMQKNEITAQTRGAIALAFQEAVVDVLTEKSLAALSKTGHAQLVVAGGVGAN
                     RQLRQNLRRRAEEIGATVFFPELEFCTDNGAMIAFAGALRLQNAKAASGDNEDKRTDV
                     FTINARWDLEMQRLPSEIG"
     gene            2043..2255
                     /gene="rpsU"
                     /locus_tag="VFI43_02935"
     CDS             2043..2255
                     /gene="rpsU"
                     /locus_tag="VFI43_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7061114.1"
                     /translation="MTTIKVKENEPFEVAMRRFKRTIEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRTYKRLRSQLLPPKLY"
     gene            2308..2754
                     /locus_tag="VFI43_02940"
     CDS             2308..2754
                     /locus_tag="VFI43_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET7061115.1"
                     /translation="MSLKQQIAEDMKDAMRKGEAKRRDAIRLLQAAIKQREVDERIML
                     DDAAIIAVIEKMLKQRRDSIAQYESANRHDLADAEKYEVRVLQAYMPQPLSESEVEAA
                     VSSAIESVGARGQQDIGRVMAVLKAELSGRADMGKVSSLVKMKLSS"
     gene            2867..4648
                     /gene="dnaG"
                     /locus_tag="VFI43_02945"
     CDS             2867..4648
                     /gene="dnaG"
                     /locus_tag="VFI43_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7061116.1"
                     /translation="MIPQSFIQDLLARVDIVDVVERHLPLKKAGANYSACCPFHSEKT
                     PSFTVSPTKQFYHCFGCSAHGSAIGFLMEYDGLSFVEAVNDLARRVGMQVPSDQVELQ
                     GHLAQEPSESSKSVQGLVEVMETAGRFYRQQLKSSNAAINYLKKRGLTGETAARFGIG
                     YAPPDWQNLDFAFEEPPAEARTELLVDAGLVIRNDDGKCYSRFRDRIMFPILNLKGKL
                     VGFGGRVLGSGEPKYLNSPETALFQKGRELYNLFHARKAIRDAGKVIVVEGYMDVVAL
                     SQHGIEHVVATLGTAITSFHVQKLLRQTDEVIFCFDGDDAGRKAAWRALEDSLDQLVD
                     GKNVRFLFLPQGEDPDSYVRKHGKNAFDELAAQSLPLSIFLFRELSARVDLKSSEGRA
                     RLVKDARPLLARVAAPGLALMLVKQLVEISGFSQRELEDLLKIRRTSFNPIRERLPRP
                     KPASPYRWLIQILLHDPGYIGKLDRELLLPDPEYAEEIAALLALIEFIDAHPHVKENT
                     AIPSCIAYFEESPHRLLLQKAESETLAWDSDIDLEAEFDGAMARLGNMRRKRRMTSLQ
                     NKALSALTPEEKQELQLLAVSWQGQPG"
     gene            4907..6919
                     /gene="rpoD"
                     /locus_tag="VFI43_02950"
     CDS             4907..6919
                     /gene="rpoD"
                     /locus_tag="VFI43_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET7061117.1"
                     /translation="MVAKSGGKSPAGMADIASAVMAAKDPTLSSKIARVKAVKEGKPA
                     QPKDGDKSSLGPQDLEARRTRLKNLIVQGKERGYLTYAEINDHLPDDMLDAEQIENII
                     SMINDVGISVYDEAPDAETLLMSETAPTVADEDVVEEAEAALSTVDSEFGRTTDPVRM
                     YMREMGSVELLTRESEIEIAKRIEDGLKHMIQAISACPTTIAGILDLADRVAKDEMRV
                     DELIDGLIDPDVDEMVSEDITEESLEQELIAESGEDEDITAVANANLLKLKNDALERF
                     AVIQQAYAEMQKALEKKGPSNKAYKDIQEKISSELMAIRFSAKMVEKLCDIQRGLVDE
                     MRGYERRIMELCVNRAGMPRNYFIKVFPGNESNSDWVNQEIAAGKPYSQALERYLPAI
                     LEQQQNLLTLQKQVGIPLKDLKEINRRMSTGEAKARRAKREMTEANLRLVISIAKKYT
                     NRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIP
                     VHMIETINKMNRISRQILQETGQEPEPALLAEKMEMPEEKIRKILKISKEPISMETPI
                     GDDEDSHLGDFIEDSATMAPSDAAVYASLRDVTKDILDSLTPREAKVLRMRFGIEMNT
                     DHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDTES"
     gene            6964..7040
                     /locus_tag="VFI43_02955"
     tRNA            6964..7040
                     /locus_tag="VFI43_02955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6998..7000,aa:Ile,seq:cat)
     gene            7567..>7709
                     /locus_tag="VFI43_02960"
     CDS             7567..>7709
                     /locus_tag="VFI43_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HET7061118.1"
                     /translation="MKTPKRIEPLVQDGLVDEVIRQLMSGKEATVYVVRCGEEIRCAK
                     VYK"
BASE COUNT         1936 a         1943 c         2143 g         1687 t
ORIGIN      
        1 atcaaggttt ttaccttgat ctcctgcaaa aaaataatgt ccattaatta tcgctgatat
       61 gccttcgcgg gttcgaacag gcccttttca cgtaaaatca gcgattttat tctatttgcc
      121 cgggcgccgc tgactagatg atgccgctgg tctttatact tctggcttat ctgcttggat
      181 ccgtttcctt cggcgtcctg gcaagctgga tcttcgattt gcccgatccc cgcacttatg
      241 gctcccgaaa tcctggcgct acaaatgtgc tgcgtagcgg aaggaaagcg ccagccgttt
      301 tcacgctcct gggtgacgcc ggcaaggggt ggctggctgt ggcggctgca caatacttcg
      361 cgccgatact cgggtttgga agctggacag tcgctctggt tgcgctagct gtttttctag
      421 gtcacctgtt tccgatattc ctgggtttcc agggtggcaa gggcgtggcg acggcgttgg
      481 gcgtgctgtt gggactcaat ccatggatgg ggctgctggc ggctgcgatc tggattgctg
      541 tggcagcgac ctggaggatt tcatcgctgg ccgcgctgac ggccgcggta ttagccccca
      601 tttatgctgt attttttctc ggcttcgaag ccaggaccgc tgcggtggtt gtgatgtcgc
      661 tattgctgat ttggcggcat aagtcaaaca ttgccggact gattgcgggt actgaaacgc
      721 ggataggcaa gcgcgggacg tcctgatcca gcgcacgcaa cttcgcacgc aactttcatc
      781 cgatttccga cggcaatcgc tgcatttcga gatcccatct agcgtttatc gtaaacacgt
      841 ccgttcgctt atcttcgtta tctccgcttg ccgcctttgc attctgcagc cgtaatgctc
      901 cggcaaacgc aatcattgca ccgttgtccg tgcaaaattc cagctcagga aaaaaaacgg
      961 tcgcaccgat ttcttctgcc cttctgcgaa ggttttgccg cagttggcga tttgctccca
     1021 ctccgcccgc tactaccaat tgagcgtgcc cggtctttga cagcgctgcc aaggactttt
     1081 cagtcagaac atcgactacg gcttcctgaa atgccaaggc gatcgcaccg cgagtctgag
     1141 ctgtgatctc gtttttttgc atcagggtgg aaacggccgt tttcaatcca ctgaagctga
     1201 agtcaagatc accgctacga agcatggggc ggggtagatg aaagtaacgg ggtgcgccag
     1261 attggagatg ttcttccgca agtctcgaga gtgccggtcc tcctggatag ccaagtccga
     1321 ggagttttgc ggttttatcg aaagcctcgc ctgccgcgtc gtcgagggtc tcgcccagca
     1381 atttatattg ccccaggcca ctcacttcca tcaattgagt gtggccgccg gatacaagca
     1441 gggcaatgaa cggaaaagcg ggcgccggtg tggacagaag aggagaaagg agatggccct
     1501 cgagatgatg tactccaaga acagggatat ttaatgcaaa gcccagagcg gcagcaatac
     1561 tgcctcccac gagcagcgcg cccgcaagcc ccggcccttg cgtatacgca atggcatcga
     1621 gatttttgag atcagcctcg actgaagcaa gggtttgccg gatgagagga agcacgcggc
     1681 gtatgtggtc tcgggaggca agctcgggca ccactccacc atattcgctg tgcatctcgg
     1741 tttgcgaata cagcgcatgg ctcagcaggc ccctgtcgct gtcgtagagg gcaacgccgg
     1801 tttcatcgca ggaagtttca acgccaagaa caagcaatcg atgtggggcg aaagaaaaag
     1861 cgcctcaaga aaaagagctt ctttggaaga aaattacctg tctgctatag tataataatc
     1921 ggctttttat gccggtacgg cgtccaaggc tgttcaatta aggctgttta attgattttc
     1981 aacggctact atacatatta attgaactct taatcttaca tttcacgagg aactgcatct
     2041 tcatgaccac tattaaagtt aaggaaaacg aaccatttga agtcgcaatg cgtcgcttta
     2101 agcgaaccat agaaaaaacc gggttgctca cagaactgcg ggctcgggaa ttttatgaaa
     2161 aaccaactgc tgagcgcaag cgcaagctgg ccgcagcggt gaaacgaaca tacaaacgcc
     2221 tgcggagtca attgcttcca ccgaaacttt actagtcctc gtcaagtcac agaatcatcc
     2281 aatttattgt ctagctggtc cctgatcgtg agtctgaaac agcagatcgc cgaagatatg
     2341 aaggatgcaa tgcgcaaggg cgaggcgaaa cggcgcgacg cgatcaggct cttgcaggcg
     2401 gcaatcaagc agcgtgaagt ggacgagcgc atcatgctgg atgatgccgc gattatcgcg
     2461 gtaatagaaa aaatgctgaa gcagcgccgg gactcgattg cccagtatga atcggcaaat
     2521 cggcacgatc tggctgacgc tgaaaaatat gaagtgcgcg tgttacaggc ttatatgccc
     2581 cagccattga gcgagagcga ggtggaggcg gcggtatcca gtgcgatcga atcggttgga
     2641 gccaggggtc agcaggatat tggccgagtc atggcggtac tcaaagcaga actttcgggc
     2701 cgcgccgata tgggaaaagt atcttcattg gttaaaatga aactgtcttc ctgacacccc
     2761 cctggatggg tttctcatca gaacgggcgt cattcttcat cattgcttga tccaagcggg
     2821 ttttgtactt ttttcagtca atgattctgt gcggtctgtt ttataaatga ttccacaatc
     2881 attcattcag gatctgctgg cccgggtaga tatcgttgat gtcgtcgagc gtcatttgcc
     2941 gctcaagaaa gcaggtgcaa attactcagc ctgctgtcca tttcatagcg aaaaaacgcc
     3001 ttccttcacc gtgagcccca cgaagcagtt ctatcactgc tttggttgca gtgcgcatgg
     3061 aagcgccata gggttcctga tggaatacga cggactaagc tttgtcgagg cggtgaacga
     3121 tcttgcgcgc cgggtaggaa tgcaagtgcc ctccgatcaa gtcgaattgc aggggcattt
     3181 ggcgcaagag ccgtctgaaa gctcgaagtc cgttcaaggg ttggtggaag tgatggaaac
     3241 ggccgggcgt ttttatcggc agcagctcaa gtcctcaaac gcggctatca attatttgaa
     3301 gaagcgcggc ctcactggtg aaaccgcggc tcgatttggc atcggttatg ctccacctga
     3361 ctggcagaac ctggatttcg ctttcgagga gcctccagcc gaggcacgta ccgagttact
     3421 tgtagatgcc ggattggtaa taaggaacga tgatggcaag tgttatagcc gattccgcga
     3481 ccgcatcatg ttccccattt tgaatcttaa aggcaagctc gtaggttttg ggggaagagt
     3541 tcttggctcg ggggaaccaa agtatctaaa ctcgccggag accgccttat ttcagaaagg
     3601 gcgcgaactc tacaaccttt ttcacgcacg taaagccatt cgcgacgctg ggaaggtgat
     3661 tgtcgtagaa ggatacatgg atgtggtcgc gcttagccag catggcattg aacatgtggt
     3721 cgcaacgttg ggtacggcca ttacttcctt tcatgtgcaa aaactgctgc gccagaccga
     3781 tgaggtgatc ttctgcttcg acggcgatga cgcggggagg aaggcggcgt ggcgggcgct
     3841 ggaagatagc ctcgatcagc ttgttgatgg caagaacgtc aggtttctct ttttgcccca
     3901 gggtgaggac cctgacagtt acgtcagaaa acatggaaaa aacgcgtttg atgagttggc
     3961 ggcccaatca ttgcccttat ctattttttt attcagggaa ctgtcagcgc gcgtcgatct
     4021 caaaagcagc gaagggcgcg cccggctggt aaaggatgca agacctctgc ttgcacgggt
     4081 agcggcgccc gggctcgcac tgatgctggt taaacagctg gtggaaatca gcgggttcag
     4141 ccagagagaa ttagaggatc tgctaaaaat caggcgaacg tcattcaatc cgattcgcga
     4201 acgtcttccg cggccaaaac ccgcctcacc ataccgctgg ctgatacaga tactcctgca
     4261 cgatccgggc tatataggaa aactggatag ggaacttctc ctacccgatc ccgagtacgc
     4321 cgaggaaata gccgccctgt tagcgctcat tgaatttatc gacgctcacc cccatgttaa
     4381 ggaaaatacg gcaataccct catgcatcgc ctattttgag gaaagtcccc accgtctact
     4441 gcttcaaaaa gccgagagcg aaacgcttgc atgggatagc gatattgatc tggaagcaga
     4501 gtttgacggc gccatggcgc gcctaggaaa catgaggcga aagcgacgca tgacaagcct
     4561 gcagaacaag gcattgagcg cgcttactcc cgaagaaaag caggaactcc agttgctggc
     4621 tgtctcatgg caggggcaac cgggatgagc agggcttggg atgagcaggg taagcgaacg
     4681 gggcaagcta aatagagtaa atagatattg atataatagg aaaattttag tttcaactac
     4741 cataatattg gaagagcaca tggtcaagcc aaaagcgcaa gttaaaacga tggaaaaaac
     4801 agaagcaaac acaaggaaat cgtcgagacc ggcaaaagca gcagaaatag agaacgtaga
     4861 cccctttgaa aaaattgaag cggaaacgct ggaaaacagg ataacgatgg ttgcgaaatc
     4921 aggcggtaaa tctccggcgg gaatggctga tattgcaagc gcagtcatgg cggccaagga
     4981 ccctactctt tcctccaaga tagcgcgggt aaaggcagta aaggaaggca agcctgccca
     5041 gccaaaggat ggggacaagt catctcttgg gccccaggac ctcgaggcaa ggcgtacgcg
     5101 cctcaagaat ctgatcgtgc aggggaagga acgcggctat ttgacctatg ccgagatcaa
     5161 tgaccatctg ccggacgaca tgctggatgc ggagcagatc gaaaatatca tcagcatgat
     5221 caacgacgtg gggatctctg tctacgacga ggcccccgac gccgaaacgt tgctcatgtc
     5281 ggaaacggcc cccacagtcg cagacgagga cgtcgtggaa gaagcggaag cggccctgtc
     5341 caccgtggat tcggaatttg gccgaaccac cgatccggtt cgcatgtata tgcgtgaaat
     5401 ggggtccgtg gaattgctga cgcgcgagag cgagatcgag attgcgaagc gcatcgagga
     5461 cgggttgaag cacatgatcc aggccatttc agcctgtccg accacaatcg ccggaattct
     5521 cgaccttgca gacagggttg ccaaggatga gatgcgcgtg gacgagctga ttgatgggct
     5581 gatcgacccc gatgtcgacg agatggtgag cgaagacatc accgaagagt cgctggagca
     5641 ggaacttatc gccgaaagcg gcgaggatga ggacattact gctgtcgcga acgcaaatct
     5701 gctcaaattg aaaaacgacg cgctggagcg cttcgcggtg atccagcagg catacgctga
     5761 aatgcagaag gcgctcgaaa agaaaggccc ttcgaacaag gcctacaaag atatacagga
     5821 gaaaatttcc tccgagctca tggctatccg tttttccgcc aagatggtgg aaaagctctg
     5881 cgacatccag cgcgggctgg tggatgaaat gcgcggatat gagcgcagga tcatggaact
     5941 gtgcgtcaat cgggcgggaa tgccgcgcaa ttattttata aaagtctttc ccggcaacga
     6001 gagcaattcc gactgggtga accaggaaat tgcggcaggc aagccttata gccaggcgct
     6061 ggaacgctat ctcccggcca ttcttgaaca gcagcagaat ctgttgacct tgcaaaagca
     6121 ggtcgggatt ccgctcaagg atctcaagga aatcaatcgc cgcatgtcaa cgggcgaggc
     6181 caaggcacgc cgggcgaaac gtgaaatgac cgaggccaac ctgcggttgg tgatctccat
     6241 cgccaagaaa tatacgaacc gcgggttgca gttcctcgat ctgatccagg aaggcaacat
     6301 cgggctcatg aaagcggtgg acaaattcga atacaggcgc gggtacaaat tctcgaccta
     6361 tgccacatgg tggattcgcc aagccatcac gcgctcgatt gccgaccagg cgcgaaccat
     6421 tcgcattcca gtgcatatga tcgagacgat caacaagatg aatcggatat cgcgtcagat
     6481 tttgcaggag accggtcagg aacccgagcc ggctctactg gccgagaaaa tggaaatgcc
     6541 ggaagagaag attcgcaaga ttctcaagat ttccaaggag ccgatttcca tggagacccc
     6601 tatcggggac gacgaagatt ctcatctcgg cgatttcatc gaagactcag ccaccatggc
     6661 gccctctgat gcagcggtgt acgccagcct gcgcgatgtg acgaaggaca tactcgactc
     6721 gctgacccca cgggaggcga aagtgctgcg catgcgcttc ggcatcgaaa tgaacacaga
     6781 ccatacgcta gaagaagtcg gcaagcagtt cgacgtgacg cgtgagcgca tccgccagat
     6841 cgaagccaag gcattgcgca agctgcgcca tccgtcacgt tccgaacggc tgcgcagctt
     6901 cctggatacc gagagctgat cgacccctgc aactgttata ctttcgcact tgcagtcagt
     6961 gcagggtctg tagctcagtc ggttagagca ggggactcat aatcccttgg tcgttggttc
     7021 gagtccaacc agacccacca aaggcttgcg tgaaaaaccg taagcctttt tctttaaatg
     7081 cggcacaaga ggggcaatat ccaacaaata gtcggcgctc gcaaggaacc aacttctaaa
     7141 cggcactaaa cgtcacaaag gaaaactcac tgaggtctgg gttgtttatg agattgagta
     7201 tctagcgatt tgttgcggtt cagaccaagt caaacggtga gggtcagtgg caactaccgg
     7261 aaagtgattt acgacgccga cttgggcgca agtttcctca agatcatcgc cgtgcgtttg
     7321 aggtctaact ccttgcttaa ccagagggct cacagcgatc cgcagttgtt tcccggttag
     7381 cccgaacgtt aggcctcaat ctaggtccca cctttacgca attcctcttt taccccaacc
     7441 accgctcgct tggcggctac tacggtccac ctgcgatgga tcttatgttt aggcacaggg
     7501 gtacttcaaa acgttgacct ccgctatcat gctatccatc gtacttgcga ccgcagcact
     7561 acatcaatga aaaccccgaa aagaattgaa ccactggttc aggatggcct tgtcgatgag
     7621 gttatccgtc agctgatgag cggcaaggaa gccacggtct acgtagtgcg ctgcggagaa
     7681 gagatacgct gcgcgaaagt ttataaaga
//
LOCUS       DASSHJ010000073        17948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1043777,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000073 DASSHJ010000000
VERSION     DASSHJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 17948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17948
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043777"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..355)
                     /locus_tag="VFI43_02965"
     CDS             complement(227..355)
                     /locus_tag="VFI43_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061119.1"
                     /translation="MTVTSVVAILVQLEKATQSYLKYDYVSVLAITNFSATFRLNG"
     gene            597..1601
                     /gene="sbnA"
                     /locus_tag="VFI43_02970"
     CDS             597..1601
                     /gene="sbnA"
                     /locus_tag="VFI43_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HET7061120.1"
                     /translation="MILRSILDGIGKTPLVSLSRLFKGSGCEVLAKLEMFNPAGSIKD
                     RPARYIIERGLVEGSISPQSHIIESSSGNFAIALAMVCRLHGLRFTAVVDPKLSVANR
                     QIIQCYGGNIELVTEKDRQGGYLETRIERVKRMLCEQPDAIWTNQYANELNWQSHYDG
                     EGEEILRALDRPVDYLVLGVSTSGTIHGITRRLREAWPGMKVIPVDAYGSILFGSPPA
                     VRELPGLGASRVPELLKRDELGQVIHIDDYESAVACRQLVKHEGIFAGGSSGSVIAAI
                     QRLSTHLLEPARILTILPDRGERYLDTVYNDQWLLRMKERHFARVSKQFVATPAGQPV
                     "
     gene            1598..2623
                     /gene="sbnB"
                     /locus_tag="VFI43_02975"
     CDS             1598..2623
                     /gene="sbnB"
                     /locus_tag="VFI43_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnB"
                     /protein_id="HET7061121.1"
                     /translation="MTISNSSPTLLYLNRADLGLLGGDHSQPYVDAIVEGLTSHARGD
                     YVQPLKPYLRWAGADHIADRIIAMPCYLGGEQPIAGLKWVGSRQHNPVRFGIERASAV
                     IVLNDTETNYPVAILEGGLISGMRTAAITGVATRHLARPGFTDVACIGCGPIARMQML
                     TLLEQFANIERIYLFDLSLDAAGALAGVLGESHGQVDFLLADSAESAVRAAEVIVTCT
                     VADSPYLAYDWLKPGVFISNVSIMDVHKDVYEKADKVVVDDWDQSNREKKIINQLVLE
                     GRFNRERLHAELGEIVIGAKPGRENDDEIILLNPMGMAIDDLICARHFYRLADERGIG
                     TRLPLYG"
     gene            2583..4451
                     /locus_tag="VFI43_02980"
     CDS             2583..4451
                     /locus_tag="VFI43_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HET7061122.1"
                     /translation="MNAESELDYRYMVEPLIMPFHPYGSGLAQDLFDALWLEDLYGFR
                     SHCSMRSIADDKAMLEVRLDSIRSLRWQGHLADGLRPFRILAPDAALRTGTEEVGLEF
                     GDAVEILQTADWWNDGTDRFASLFHLAYDQATFTAAHERNIVARLLAVPRDLLSWEAL
                     SCLKDRPFHPLARAKDWNEAPGHAYAAETMALLPLRWVAVPRNRIVTASGNRSTGQPL
                     VENLLDPAQQDILANAAHACHADDEAWLWMPVHPWQWSWLKRSSPAKLNGCIELCTGP
                     GTAIPTASLRSLVIPGKPDTHLKVALSVRTLGAVRTLPLRYLHNGVLASACLETLRKR
                     DAWLGANLLLCQENEWWALSQSLQRHQPGQYDTLVSEPGELACMLRRYPPLPGATLIP
                     MAALPATTADGALPAFDHLVGTIKQEEDAWRLFRDIARALLELGLRCFAHGVMPELHG
                     QNVLLAFKDRRIAALVLRDHDTLRVCQGMLEQRGIKAPDYVIDRSTPNTLELDTPWEL
                     LAYLQTLAIEVNLYAIIVALASHYSRDEAQGWHIVRQVLEICLASIPLPPEIASQTKN
                     LLLNEAEWPFKQVLAPLLGAESFGTGMPSAMGRVKNPLLTGNLPSYGGGSRDISYA"
     gene            4444..5628
                     /locus_tag="VFI43_02985"
     CDS             4444..5628
                     /locus_tag="VFI43_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7061123.1"
                     /translation="MHNLTLLLVCQAVTTLGLMVFVPIMPLYIETLAGVDTVDAAYWS
                     SLALAAPAVGTLCLASFAGKWCDRFGYRPMLLASLGVFIASMLLMALSPSVHGFMLGR
                     LLQGVSAIGVVLTAFIGYVSDDASRGRSLGLQESAIACGSLAGPILGGLMLDSWSLKP
                     LLLWSAVITGIAGGILWTQLSEPVKALPISGSSFSGLRTMLSHAYLRNWMLAACLIQA
                     AGFAFVNAFPLYLTTLFPRSEAMASKIGLLHALGWLAAMLAAPMWGHLNDRGEPERYF
                     VLAAMGCALSASLLAVVDQIWLVVLLRLSQGACYAALMQSVFLACSRQSPASSHGHVT
                     GLSRSFMVVGQLLGPLLIMLLLPILSPINLVWPVAFLFFLAGLLVLNARIAEYPLAEA
                     EK"
     gene            5625..7394
                     /locus_tag="VFI43_02990"
     CDS             5625..7394
                     /locus_tag="VFI43_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HET7061124.1"
                     /translation="MMPMPSYQPELAPIERRLVEQYLNTYCRETACFDPRLAADDQGP
                     AELHSQINTWRASGWMPYLVTFGQTQYQLAGAFTYFSPIGYHRLAPCAWIGLNGDWQA
                     LTTDATPFTDLIADALVAMSNPAGRNQPGSSRPGEMEESGSRLKALMRNSIERVRRFH
                     LHKPARGCPPFVTAEQGLRFGHIFHVTSKAAEGFSEEDIQRYAPELGASFRLHYFAVS
                     ADILETRIVGGQHLAVDPEVALFTKDLLCGGNCELIPCHPWQANYLLGLAEIKALIES
                     GKLISLGPQGEVAWPTSSLRTVWLPRQNLFLKLSLNIRITNFIRNNPSEQVRRALDAS
                     LALSMLPRHFLEQPTFRILPELASQTLRLPTESLRAACAVVYREAMPPTEAEHVQTIV
                     TVLEEPAHGEIPLLALLRLSAARKPLREPLVRSWWKHYLDATLLPLLRLFMGYGVSLE
                     AHLQNTLIAFHNGWPVRGYVRDMEGASISRERFPFSGRLPGDSPALYPEEQAWHRFQY
                     YVLVNHIGHVIACLAREGLATEAMLWRDTAFALSTIDDPLIRRLLIQPTLPAKANMLS
                     SFYQRGESPSWVEIPNPMAQRQE"
     gene            7391..9187
                     /locus_tag="VFI43_02995"
     CDS             7391..9187
                     /locus_tag="VFI43_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HET7061125.1"
                     /translation="MNPVDYKNLINSLAYRQASRRVLRQLLEALLFEGAVSHSVWNGE
                     TLSLPGLRVDRVPVQYQCQARHTFSFGRVRVMTPLLRIDGGVTQEASDPALFLAEIAP
                     WLAAERNRVGEFAAELLATQIKHAQTLSAREGVPLRDADYDTVEARLTDGHLYHPAYK
                     SRIGFTLNDNAMYAPEIACFVAPLLLAVRKDCCRWGASSKLTGKEKQAPGLGSFLLGE
                     AGWTYFCDELAKLALQSENYMPLPVHPWQWDRVLVSGYHQAMARGEILVIGALTDRYL
                     PQQSIRTLSNLSDRNRLSLKLAMNLVNTSTSRVLAPHTVQNAQLISDWLHELVERTDW
                     QLPLARPVMLGEVAGVSFMPLAPAHGQYGALSCIWRESVHSHLRPGESAVPMTAVTHI
                     DIDGKPFIDPWVQRYGPEEWLTRLVERVYLPVLHLLWEHGTALESHAQNMVLLVEEGL
                     PVRVALKDFHDGVRFCKSLLSRPAPALIAPPPQHAQVNPNSFLETESADELRDFTFDA
                     LFFVNLAELAWLFQSHYAIAEKRFWQVTREVLQAHQKRNPQSAARYALFDCLADEVGI
                     ELLAARRFQPEIRLRTRLTPNPLAQPGEAPCI"
     gene            9178..9939
                     /locus_tag="VFI43_03000"
     CDS             9178..9939
                     /locus_tag="VFI43_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7061126.1"
                     /translation="MYLKKRLQANETVYGVFCSTPAALSIEIIAAASYDFVVIDLEHT
                     LIDARQLAAMLLAARSSRMAALVRVASGYQALQVLDAGAEGVVFPRVASAQAAQEAVR
                     ACYFAPLGERGLNATWHSGYGRDDLAEFTNGANEHTLVVAMIEDSEGLGNVEEIAAVE
                     GIDVLLEGAADLSQSFGLPWQTRHPDVRAAISQIRAAARAHDRTFCAIPRVPHDCASW
                     HCDGVHMFILGDDRGITRRAMAAHLMQHQIQVFKP"
     gene            9936..11171
                     /locus_tag="VFI43_03005"
     CDS             9936..11171
                     /locus_tag="VFI43_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HET7061127.1"
                     /translation="MNLQHVRDYLDGHARRRTQSSERHASGIPDPQPVCAYLYDLAHL
                     GRRASRLRCRLPAQCKLFYAVKANSDEPILKSLLDQTDGFEVASLGEIQRVRAVDDSV
                     LIVFGGPGKSDMELEGALEHRVMLIHVESEHQLRRLDWIARFRNQTADILLRVNPAHV
                     PQQATLRMGGQPIQFGVEENRIPDLFELARNLHGIRLKGLHFHAISNNLDAAAHLALV
                     EHYFNLAEAWQRRYGLKFGWLNVGGGIGINYADPGSPFDWENFCAGLSALIAARQPCF
                     QVIFECGRFLAADCGCYVSEVLDVKTAYGKHFAILRGGSHHFRLPVSWQHNHPFVILE
                     RDSWRYPFPRPGVHDAPVTLCGEQCTPKDTLARDIHVERLRAGDCVAFLLAGAYGWHI
                     SHHDFLSHPHPERVFLDNY"
     gene            11185..11715
                     /locus_tag="VFI43_03010"
     CDS             11185..11715
                     /locus_tag="VFI43_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET7061128.1"
                     /translation="MLCSLPIDFVPVDQIRPNECHYPDHAVTLADTILREQLWRVPVV
                     LERFSLAVMDGHHRLEAARKLRLKYVPCLLLDYDSVEVDATRPGYVVNPCEIIRRARA
                     SDLYPPKTTRHRFPSPLPICNISLLLLEGHAAIAFPAASTRFSNHCAATTVEAANDRT
                     STSDVPVECRVNGEAS"
     gene            11712..13814
                     /locus_tag="VFI43_03015"
     CDS             11712..13814
                     /locus_tag="VFI43_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HET7061129.1"
                     /translation="MKMYLRSAVAAFSLASSIQQAGAQTIAKDGPESGSKLPAVTVMG
                     ERTGSFKSDTVQVGTFRDMAPLDVPQTSNVITREVLDAQATTGLFGALRNTAGVTRSQ
                     LNGSTYDNISIRGILVENRSNYRLNGSLPVINLIDIPMENKERVEVLKGASSLYYGFV
                     PPSGIVNMVTKRAGKDPVTNLSMLLNQYGGANMHIDVGRRFGDQQQFGARINLLVGRE
                     DIGIHNFSGYRALTSGAFDWRVNDKLSFKFDVEHYRKEVSEQAAIQAPAAVNGVITLP
                     DVPDNRLNLAGEWQKYDAEATNLLLRGDYAINDNWGLLFEVGKARTTRDRNFSQFQNY
                     NLVTGQGTLRTFFNRGQEFDNENVRAELFGRLPGKWITHEASVGFTGNERSQDPRTSS
                     SVDIPQNLYNPIDIAKRSPTVPFRSNSSKITDFGFYLFDRVFIGDKLQAMLGVRGSFY
                     ESVAATTHFKADSVNPALSLLYKPLPNISIYGSYLEGVEESGQAPANRANAGQLLAPA
                     LSTQKEIGIKAVVAQGILLQVAAFDIKRASTTVDSANRFVLNGMAQYRGIELAASGEI
                     TKQLSVIASALFMDARQLNRANAETFDQIPDNTPERTASMFAEYRLQSIPGLALSSGV
                     YYVGRRPVNNANQAFVDDYVLLSLGARYASRVAGKRTTIQAFLDNATNEDYWSTAGNG
                     LLGVGPPILLKIAGRVEF"
     gene            13985..15145
                     /locus_tag="VFI43_03020"
     CDS             13985..15145
                     /locus_tag="VFI43_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HET7061130.1"
                     /translation="MKIRRIVFNFHLYAGLAAGLLLVLSGLTGSMVVFREEIEELMYP
                     ELLETVVRDEQVPIQAVLSAAREAYPRDKLLSIRMPRAPQQTYLLKMNGPHDLFVYAD
                     PYTGAMLGSHRQEDTFMGWIALVHTELLIGERGKNILGLSALLLVCMIVTGFFLWWPP
                     GGKERNGRARKILRGFKIDLSGPWKKMLFDTHRAIGIYTSIFLLIVAFTGVSLVFNKA
                     VAELANLLTASPPRPAPPVSIAVATGRETPSLDELLPKADRVLPGAVTWINFPQTPQA
                     PLVVRKKLPEELHPNGRSFVYFDQYSGEVLFVEKASASPIGSRIYNTFYPIHTGVIGG
                     AYTRILQVIVGFSPLVLLVTGYVMWRNRKKAKAYKAGSRKLGFNSVGLGRDK"
     gene            15590..16018
                     /locus_tag="VFI43_03025"
     CDS             15590..16018
                     /locus_tag="VFI43_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061131.1"
                     /translation="MKSSILTLFFLLTFSSIASAQAIPPPDPRSPPRPQDVRIAQLQT
                     RLNAINQAQQSMYQQFQMLQEMRRNEIAGTFPQVLPGGGLSNLPPISYDETVRLQRER
                     QDRLQRYERDLDELYARYAELDEQKKAILDEIVELAKPEE"
     gene            16054..16461
                     /locus_tag="VFI43_03030"
     CDS             16054..16461
                     /locus_tag="VFI43_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7061132.1"
                     /translation="MQILEAAVSTVKAGNVKMKRAYEAAEPTDGIRILVDRLWPRGVS
                     KDEAAVDQWMKDVAPSTELRKWFGHDPDRWDEFCKRYVAELEQHPDQLKELRELARKS
                     PITLVYSARDETHNDAVVLRDFILRRVKSVDHE"
     gene            complement(16559..17440)
                     /locus_tag="VFI43_03035"
     CDS             complement(16559..17440)
                     /locus_tag="VFI43_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7061133.1"
                     /translation="MKFTATFSCIAAWLICLFAYTLQVPRVLAQSAPTIEFRKDNQTV
                     KTFSVDDLAAIAPPVPLKVFEAHEKEERVYRAIPARPVFERIFGKNWEKAQQIVFTSI
                     DGYQPSVPVEKFLAHNAYLALAHDDGSPFIMLNKLQNNEKVPLGPLYLIWDNIGSKAL
                     LESGASDMPYQIKTIEIKSEDPFAGLAPPKGSSKQVLRGFDHFRKYCVSCHTINGEGG
                     GKAPELNYPVSVSEYIKPEYLKRWIEQPQTIRYNTMMPALGHEVPNRDKVADEIIAYL
                     KMMSIRKRVPEKSSGKQ"
     gene            complement(17505..>17948)
                     /locus_tag="VFI43_03040"
     CDS             complement(17505..>17948)
                     /locus_tag="VFI43_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7061134.1"
                     /translation="LLNEWNVSPNRLIIELTETAAVSDIQDAQRFIEAIGHTGCRVCL
                     DDFGSGFSTFGYLKYLNAQILKIDGLFICNLPDNRDNQIFVKAMVEVARGLGKITVAE
                     CVEDAATLDMVRDLGVDMVQGYYLERPKPQSAMQLQFDAGVAEPE"
BASE COUNT         3839 a         4901 c         5044 g         4164 t
ORIGIN      
        1 aattactccg ataataatat atccttaccc agcgagagtg aaagtattaa cgttgacttg
       61 tcgcaatatc cacacaagca tggcacttta gttcgatgta cgcgaagcgg atgtttcact
      121 gaaagccgga agctaaaact cgaagattta tttgctttca atcgctcttt ataagtagtt
      181 atacgttata gctcgccagc ccgtccaggg caagccagcc aaatttttat ccgttaagcc
      241 ggaacgtcgc ggagaaattg gtaatggcca agacactcac ataatcgtat ttcaagtagc
      301 tctgcgtcgc cttttccagt tggacaagga ttgcgacgac tgaggttact gtcattgctg
      361 cgccaagagt tcggcaagag ctcgctaatt ccagtagttg cacttgatta ttttaggggc
      421 cggagctgga aggctgtcga gggcaaccag ccggctaaaa gctggaacac aaggcggaaa
      481 cggacataag gctggaaatg aggtgccgaa agggcgttct acctataaat acatctcatt
      541 cttgttatca tttttatttt tactaagatt atcttgactg attcaaaggg atcaaaatga
      601 ttcttcgcag cattcttgat ggcataggaa agactccttt agtcagcctc tcccgcttgt
      661 tcaagggttc gggttgtgaa gtactggcca aactcgagat gttcaaccca gcgggcagca
      721 ttaaagacag acccgcgcgc tacatcatcg agcgagggct ggtggaaggc tccataagcc
      781 cccagtcgca cattatcgaa agctcctctg gaaatttcgc gattgccttg gctatggttt
      841 gccgcttgca tggattgcgt ttcactgccg tagtggaccc caagctttcc gtcgccaatc
      901 gccagattat tcaatgttac ggcggcaata tcgaacttgt tacggagaag gatcgccagg
      961 gcggctatct ggaaactcgt atcgagcggg tcaagcgcat gctgtgcgag cagcctgacg
     1021 caatatggac caaccagtat gccaacgagc ttaactggca gagccattac gacggggaag
     1081 gggaggagat tctccgtgca ctggatcgac ccgtcgatta tctggttttg ggcgtcagta
     1141 cctcaggcac tatccatggc attacccggc gtttgcgcga ggcatggccc ggcatgaagg
     1201 tcatcccggt cgacgcatac ggctcgatat tattcggttc accgccagcc gtccgcgaat
     1261 tacccggact cggtgcgagc cgcgtacccg aattattgaa gcgggacgaa ctggggcagg
     1321 tcattcacat tgatgactat gagtcggccg ttgcttgtcg gcaattggta aagcacgaag
     1381 gaattttcgc tggaggctcg tccggatctg tcatcgcagc tattcaacgg ctctccaccc
     1441 atctgctcga gcctgcgcgc atcctcacca ttctccccga ccggggggaa cgctatctgg
     1501 acacggtata caacgaccaa tggcttttac gcatgaaaga gcgccatttt gcgcgtgtat
     1561 caaagcagtt tgttgcaact cctgcaggac aaccagtatg acaatttcaa attcctctcc
     1621 caccttgctc tacctcaatc gggccgacct cggcctactg gggggcgatc actcgcaacc
     1681 ttatgttgac gcaatcgttg agggattgac atcgcatgcc cgtggtgact acgtgcagcc
     1741 gctcaaacct tacctgcgct gggctggggc cgaccatatt gcggaccgca ttatcgcaat
     1801 gccttgctat ttaggtggtg agcagccgat cgcgggcctg aaatgggtag gcagccgcca
     1861 gcacaatccc gtcaggtttg gtattgagcg tgccagcgcg gtcattgtgc tgaacgatac
     1921 ggaaaccaat tatccagtcg ctattctcga gggggggctg atcagcggta tgcgaactgc
     1981 cgccattact ggtgtagcta cccgacacct ggcgcgcccc ggttttaccg acgtggcctg
     2041 tattggctgt gggccaatcg cacgcatgca aatgctgacc ttgctggagc agtttgcgaa
     2101 tatcgagcgt atttatctat tcgatttgtc tcttgatgcc gccggcgccc tcgctggcgt
     2161 gctgggggag agccatgggc aagtagattt tctcctggct gactcggcag aatcggcggt
     2221 acgagcggct gaggtgatcg tgacctgtac ggttgccgat tctccttacc tggcctacga
     2281 ctggcttaaa cccggcgttt tcatcagcaa cgtgtcgatc atggacgttc acaaagatgt
     2341 ctatgagaag gccgacaagg tggttgtaga cgattgggat cagtccaacc gcgagaaaaa
     2401 gataatcaat caactggtac tggaaggccg cttcaaccgc gagcgcttgc atgcggaact
     2461 gggcgaaatc gttatcggag ccaagcctgg ccgggagaat gatgacgaaa taatcttgct
     2521 caatccaatg ggcatggcga tcgatgatct catctgcgcc cgccatttct atcgcctggc
     2581 ggatgaacgc ggaatcggaa ctcgattacc gctatatggt tgagccgctc atcatgccct
     2641 ttcatcccta cgggagtggt ctggcacagg acttgttcga tgcgctctgg cttgaagacc
     2701 tttacggctt ccgctcgcat tgcagcatgc gttctattgc ggatgacaaa gcaatgctgg
     2761 aagtgagact cgacagcata cggtcattgc gttggcaggg gcatctggct gatggtttga
     2821 ggccatttcg gatcctcgct cccgatgcgg cgctgcggac gggcacggag gaggttggtc
     2881 ttgagtttgg cgatgccgtc gagatattgc agacggcaga ttggtggaat gacgggacag
     2941 accggtttgc aagtttgttc cacctcgcat atgaccaggc taccttcacc gcggcgcatg
     3001 aacgcaatat cgtggcgcgc ctcctcgccg taccccgcga cttgctatcg tgggaagcct
     3061 tgagttgcct gaaagatagg ccatttcatc ccctggctcg tgcgaaggac tggaatgaag
     3121 cccccgggca cgcatacgcc gccgaaacga tggctttgct cccgctccgt tgggttgcgg
     3181 tgccacgaaa tcgaatagtg acggcatcag gaaaccgatc cacaggccag ccgctggtgg
     3241 aaaatctgct cgaccccgcg cagcaggata tattggctaa tgcggctcat gcctgtcacg
     3301 ctgatgatga agcctggtta tggatgccgg tccatccatg gcaatggtca tggctcaaac
     3361 ggtcgagtcc cgcaaagctg aatggctgca ttgagctatg tacagggcct ggaacagcca
     3421 ttcccaccgc ctcgctgcgc tcgctcgtca tccccggaaa gccggatacg catctcaagg
     3481 tggcgttatc tgttcgcacg ctcggtgctg tccgtaccct tccattacgc tatttgcata
     3541 acggggtgct cgcgagcgcc tgcctggaaa cattacgtaa acgcgatgca tggctgggag
     3601 cgaatctttt gctatgccag gagaacgaat ggtgggcgct gagccagagc cttcagcggc
     3661 atcagccagg gcaatacgac acactcgtat ccgaacccgg tgaactcgcc tgcatgctcc
     3721 gccgctatcc cccattgccc ggtgcaacgc tgattccaat ggcggcattg ccggccacca
     3781 cggctgatgg tgcgctccct gctttcgatc atctagtcgg tacgataaaa caggaagagg
     3841 atgcctggcg cctgttccgc gatatcgcca gggcgttact cgagctgggc ctgcgatgtt
     3901 tcgcgcacgg cgtgatgccg gagcttcatg ggcagaatgt cttgctggcc ttcaaagacc
     3961 ggcggatcgc agcactggtt ctgcgcgacc acgatacgct gcgcgtatgc caggggatgc
     4021 tggaacagcg tggtatcaag gcgccagact atgtcatcga tcgcagtacg ccaaacacgc
     4081 tggaactgga tacgccttgg gaactgctgg cctacctgca gactttggcg atcgaggtca
     4141 atctgtatgc catcatcgtc gcactggcca gtcactacag ccgggacgag gcccagggct
     4201 ggcacatcgt tcgccaagtc ctcgaaatct gccttgcctc cattccccta ccgcctgaaa
     4261 ttgccagtca gacaaaaaac ctgttgctga acgaagccga atggcccttc aaacaagtgc
     4321 ttgctccact gcttggcgct gaaagtttcg ggacgggcat gcccagcgca atgggccgag
     4381 tgaaaaatcc tctgctgacc gggaaccttc caagttacgg cggtggatcc cgcgatatca
     4441 gttatgcata acctcacgtt gctgctggtc tgccaggccg ttacgactct tggtctgatg
     4501 gtttttgtgc cgatcatgcc gctgtatatc gagacgctgg caggggtgga tacagttgat
     4561 gcggcttact ggagcagtct cgcactggca gcaccggcgg tgggcacatt gtgcctcgct
     4621 tctttcgccg gaaaatggtg cgaccgtttt ggctacagac caatgcttct tgcgtcactg
     4681 ggcgtgttca ttgccagcat gttgctgatg gcattgagcc ccagcgtgca tggtttcatg
     4741 ctgggccgtt tgctgcaagg cgtgagcgcc attggcgtgg ttcttaccgc attcatcgga
     4801 tatgtgagtg acgatgcctc gcgcggacgc tccctgggtt tacaggagag cgccattgcc
     4861 tgcggttccc tggcgggccc catattagga ggtctgatgc tggactcctg gtctctcaag
     4921 ccgctgctgc tgtggagcgc cgtgattacg ggtattgcag gcggaatatt gtggacccag
     4981 cttagcgaac cggtaaaagc attaccaatt tccggatcat ctttctccgg tttgcgcacc
     5041 atgttgagtc atgcgtattt gcgtaactgg atgctcgccg cctgtttgat ccaggcagcc
     5101 ggattcgcgt tcgtgaatgc cttcccgctt tacctgacga cgcttttccc gagatctgaa
     5161 gcaatggcga gcaagatcgg actgttgcac gcgttgggat ggctggccgc catgctggct
     5221 gccccgatgt gggggcatct gaacgaccgg ggcgaaccgg agcgttattt tgtgcttgcc
     5281 gcgatgggct gcgcgctatc tgcgtccctg ctggcggttg tcgatcagat ctggcttgtc
     5341 gtcttgcttc gcctgtccca aggcgcttgt tatgcggcac tgatgcaatc cgtatttctg
     5401 gcctgcagcc gtcaatcacc tgcatcctca catgggcatg tgacgggatt gagcagaagc
     5461 ttcatggttg tcggccagct gctgggcccg ctgcttatca tgctcctcct gcccatcctt
     5521 tcgcctatca atctcgtctg gccggttgcc ttcctgttct tcctcgccgg tttgctcgtt
     5581 cttaatgctc gcatcgctga atatcccctt gcggaggcgg agaaatgatg ccaatgccgt
     5641 catatcagcc ggaactggcg cctatagagc ggcgcctggt cgagcagtac ttgaatactt
     5701 attgccggga aaccgcttgt tttgatccgc ggttggcagc agatgaccaa gggcctgctg
     5761 aacttcattc tcagatcaac acctggcgag cgtccggatg gatgccctat cttgtgacgt
     5821 tcgggcaaac ccaataccaa ttggccggcg cgtttaccta tttttcacct atcggctatc
     5881 accggctggc tccctgcgcg tggatcggac tgaatggcga ctggcaagcc ttgaccaccg
     5941 atgcaactcc attcaccgat ctgattgccg acgcacttgt tgctatgagc aacccggccg
     6001 ggaggaatca gcctgggagc agtcgccccg gggaaatgga ggaaagcggt agtcggctga
     6061 aagcattaat gaggaatagc attgaacgag tccgccgttt tcatctgcac aagccagccc
     6121 gcggatgccc gcctttcgtc actgccgaac aaggcctgag gttcgggcat atttttcatg
     6181 ttacgtcaaa ggcggcggag ggtttcagcg aggaagatat tcagcgctat gcccctgagc
     6241 tgggcgcctc gtttcgcctg cattacttcg ccgtaagcgc cgatattctg gaaacccgta
     6301 tcgtaggcgg ccagcattta gccgttgacc cggaagtagc cctgttcaca aaggaccttc
     6361 tttgtggagg caattgcgag cttataccct gtcatccatg gcaggctaat tatctgctgg
     6421 gcctggcgga aatcaaggca ttgatagaga gcggcaagct tatctcgctg ggtccacaag
     6481 gtgaagtggc atggcctacc tcctcgctgc gcacggtttg gctaccccgc caaaatctgt
     6541 tcctcaaact gtcgctcaat atcaggatca ccaatttcat aaggaataat ccgtccgagc
     6601 aggtgcggcg tgcgttggat gccagcctgg cgctatcgat gcttcctcgc cacttcctgg
     6661 aacaaccgac attccgcatt ctcccggaac tcgccagcca gactctgcga ttacccactg
     6721 aatccctgcg tgctgcatgc gcggttgttt atcgcgaggc gatgccgcca accgaggcgg
     6781 agcacgtcca gacaatcgta acggtgcttg aagagccggc tcacggcgaa attccattgc
     6841 tggctttgtt gagattatcc gcggcgagaa agccgcttcg cgagcccctg gttcgcagct
     6901 ggtggaaaca ctatctcgat gcgacgctgc tgcctctact gcgcctgttt atggggtatg
     6961 gggtcagtct tgaggcgcac cttcaaaata cgctgatcgc ttttcacaat ggctggcccg
     7021 tgcgtggtta cgtccgcgac atggaggggg cgagtatcag ccgcgaacgc tttcctttct
     7081 ctggccgcct gcctggggat agtccagcgc tctatccgga agagcaggca tggcatcgat
     7141 ttcagtatta cgtacttgta aaccatattg ggcacgtaat tgcctgcctt gcccgtgagg
     7201 gattggccac tgaagccatg ctgtggcgcg acacggcatt cgcgttgagc acaattgacg
     7261 acccactgat acggcgactg ctgatccagc caaccttgcc tgccaaagca aacatgctca
     7321 gcagctttta ccaacgtggg gaaagccctt catgggttga gatacccaat cctatggccc
     7381 aaagacaaga atgaatccgg ttgattataa gaacctcatc aatagccttg cttaccgaca
     7441 ggccagccgc cgcgtcttac gtcagctgct ggaggcgctg ctattcgaag gggcggtatc
     7501 acattccgtg tggaatggcg aaacactcag tttgccgggc cttcgggtgg accgcgttcc
     7561 cgtgcagtat caatgccagg cgcgtcacac tttcagcttt ggccgcgtgc gcgttatgac
     7621 gccattattg cgtattgatg gcggcgttac ccaagaagcc tccgacccag ccttattcct
     7681 ggcggaaatc gcgccgtggc tggctgccga gcgaaaccgg gtcggcgaat ttgcggccga
     7741 gctcctggcc acgcaaatca agcatgcaca gacactgtct gcgcgcgagg gcgtgccgct
     7801 acgcgatgcc gattacgata ccgtggaggc gaggctgacc gacggtcacc tctaccatcc
     7861 tgcatacaag tcgcggattg gctttacatt gaatgataac gcgatgtacg cgccggaaat
     7921 tgcatgtttt gtcgcaccgc tgctcctcgc cgtacgcaag gactgttgcc ggtggggggc
     7981 cagcagcaaa ttgaccggga aggaaaagca agcgcccggc cttggctcct tcctgcttgg
     8041 tgaagccggt tggacatatt tttgcgatga actggcaaag ctcgcattgc aatcagagaa
     8101 ctatatgccg ctgccggttc acccatggca gtgggataga gttctcgtat ccggttatca
     8161 ccaggccatg gcccgcggag aaatactcgt cattggcgcg ttgaccgatc gttatctccc
     8221 ccaacagtcc attcgcacct taagcaatct cagtgaccga aatcgactgt cgctcaaact
     8281 ggcgatgaat ctcgtcaata cgtcaacgtc gcgggtactg gcgccgcata cagtccagaa
     8341 cgcacagctc atcagcgact ggctgcatga actggtagag cgcaccgatt ggcagttacc
     8401 tctggcccgt cctgtgatgc tgggcgaagt agccggcgta agcttcatgc cgttggcccc
     8461 cgcccacggc caatacgggg ctttgtcgtg catctggcgc gaaagcgtgc acagccactt
     8521 gcgaccgggt gaaagcgccg taccgatgac tgcggttaca catatcgata tcgatggaaa
     8581 acccttcatc gacccttggg tgcaacgcta cggtcctgag gaatggctga cccggttggt
     8641 ggagcgtgtg tatctgccgg tgctgcatct gctttgggaa catggaacgg cgttggagtc
     8701 tcatgcccag aatatggtcc tgctggtgga agagggtttg ccggtacgcg tggcgctcaa
     8761 ggatttccac gacggggtcc gtttctgcaa gtcactcctg tcacggcctg cgccggcgct
     8821 catagcaccc ccacctcagc atgcgcaggt caatcccaat tccttcctcg agacggaaag
     8881 cgctgatgaa ttaagggatt tcactttcga cgcccttttt ttcgtcaacc tggccgaact
     8941 ggcctggttg tttcaaagcc attacgccat cgctgagaaa aggttctggc aggtaacccg
     9001 tgaagtactg caagcccatc agaaacgtaa tccgcaaagc gcggcccgtt acgcgctgtt
     9061 cgattgctta gcggatgagg taggaatcga gctgcttgcc gcccgccgtt ttcagccgga
     9121 aatccgcttg cgtacgcggc ttaccccaaa cccgctggcg cagcccgggg aggctccgtg
     9181 tatctgaaga agcgcttgca ggcgaacgaa acggtgtacg gggtgttctg ttcgactccg
     9241 gccgccctga gcatcgagat catcgcggcc gcgagttatg actttgtcgt aattgacctg
     9301 gaacatacat tgatcgatgc gcggcagctt gcagccatgc tgctggcagc tcgatccagc
     9361 cgcatggctg cgctggtgcg ggtggcaagc ggttatcagg cgctacaggt gctggatgcg
     9421 ggcgcggaag gggtggtatt tccaagggtt gcttcggcgc aagcggcgca ggaggccgta
     9481 cgtgcctgct atttcgcgcc acttggggag cgcggcctga acgcgacatg gcatagcggt
     9541 tatgggcgcg acgacctcgc ggagttcacg aatggtgcaa atgaacatac tttggtggtg
     9601 gcaatgatcg aggacagtga agggctgggt aacgtggagg aaatcgcggc agtggagggg
     9661 atcgatgtgc tcctggaggg cgcggcagat ctctcgcaat cattcggtct gccttggcaa
     9721 acgcggcatc cggacgtgcg ggcagcaata tcgcaaattc gggcggcagc acgagcccat
     9781 gacaggacat tctgtgcaat ccctcgcgta ccccatgatt gtgcttcctg gcattgcgac
     9841 ggagttcaca tgttcattct cggtgatgat cgcggcatta cacgccgagc gatggcagct
     9901 cacttgatgc agcaccagat ccaggttttc aagccatgaa tcttcagcat gtgcgtgatt
     9961 atctcgacgg ccacgcgcgc cgccgaacac agtcttccga gaggcatgct tccgggattc
    10021 ccgacccgca acccgtatgt gcctatctct atgatctcgc gcaccttggg aggcgagcaa
    10081 gcagactgcg ctgccgcctg cctgctcagt gcaagctttt ttacgccgtc aaagcgaaca
    10141 gcgacgagcc gatattgaaa tcgctgctcg atcaaaccga tggttttgaa gtcgcgtcac
    10201 tgggtgaaat ccaacgggta cgcgctgtcg atgactcggt tctcatcgta ttcggtggcc
    10261 ctggcaaaag cgatatggaa ctggaaggcg cattggagca tcgcgtcatg ctgattcacg
    10321 tggagagcga gcatcagttg cggcgcctcg actggattgc gcgattccgc aatcagaccg
    10381 cggatatcct gctgcgtgta aatccggccc acgtcccgca gcaagcaaca ctccgaatgg
    10441 gcgggcagcc aatccagttc ggagtcgaag aaaaccgtat tcccgatttg ttcgagctgg
    10501 cgcggaacct gcacggaatc cggctcaagg ggctgcattt tcatgccatt tccaacaacc
    10561 tggacgccgc cgcccatctt gctctcgtag agcattattt taacctggca gaggcgtggc
    10621 agcggcgcta tgggttgaag ttcggctggt tgaatgtcgg cggcggcatt ggtatcaatt
    10681 atgctgatcc cggttctcct tttgactggg aaaatttctg tgcgggacta tcagcactca
    10741 ttgccgcacg ccaaccctgc tttcaggtga tcttcgaatg cggacgcttc cttgccgcag
    10801 actgcggttg ctacgtcagc gaagttctcg atgtgaaaac cgcttacggg aagcacttcg
    10861 ccatactgcg cggcggatcg catcacttcc ggctgccggt ttcgtggcag cacaaccatc
    10921 cctttgtcat actggagcgc gatagctggc gctacccgtt tccacgtccc ggagtgcatg
    10981 acgcgccagt cacgctttgc ggcgaacagt gtacccccaa ggacaccctg gcgcgggata
    11041 ttcatgtcga acgcctgcgt gcaggcgact gtgtcgcatt cctgctggcc ggggcttacg
    11101 gctggcatat ttcgcatcat gattttttaa gccacccgca tcctgaacgc gtttttctcg
    11161 ataactatta gaaaaaggaa cgtcatgctg tgctcgctcc cgatcgattt tgtgcctgtc
    11221 gaccagatcc ggccaaacga atgtcattat ccggatcatg ccgtaactct ggctgacacc
    11281 atcttgcgcg agcaactctg gcgtgtcccg gttgttcttg aacgtttcag cctggcggtc
    11341 atggatggtc accatcgcct ggaagcagcg cgcaagctcc ggctgaaata cgttccctgc
    11401 ttgttgctcg actacgatag cgtggaggtc gacgcaaccc gtcctggtta cgtcgtcaat
    11461 ccttgcgaaa tcatccgtcg tgccagagcc agcgacctat acccgccgaa aacgacgcgc
    11521 cacaggtttc cgtcgccact tccgatctgc aatatttcgt tgctgctgct cgaaggccac
    11581 gcggcaatag ctttccccgc agcatcgact cgtttttcca atcattgcgc ggccacaacg
    11641 gtcgaagctg ccaacgaccg cacgtcaacc tctgacgtcc ccgttgaatg ccgagtcaat
    11701 ggggaagcat catgaaaatg tatctgcgtt cggcagttgc tgcgttttcc ctggcatcat
    11761 caatccagca agccggcgcc cagaccatag ccaaggatgg gccagaatcg ggaagcaagc
    11821 ttcccgctgt aaccgtcatg ggcgagcgta cgggaagctt caagtcggat accgtccagg
    11881 ttggaacatt tcgcgatatg gcgccactgg atgttccgca gaccagcaac gttatcacgc
    11941 gcgaagtcct cgatgcgcag gccacgaccg gactattcgg cgcattgcgc aatacggcgg
    12001 gcgtcacgcg ttcacagttg aacggatcga cctatgacaa catctcgatc cgaggcatcc
    12061 tggttgagaa tcgcagcaat taccggctca atggatcgct accggtcatc aacctgattg
    12121 acattcccat ggagaacaag gagcgcgtgg aagtgctcaa gggtgcttct tcactgtatt
    12181 acgggttcgt cccgccgtcc gggatcgtca acatggtgac aaagcgcgca ggcaaagatc
    12241 ccgttaccaa tctttcaatg ctgctcaatc aatatggcgg ggcgaacatg cacatcgacg
    12301 tgggcaggcg ctttggcgac cagcagcagt tcggtgcgcg gatcaacttg cttgtcgggc
    12361 gtgaagacat tggaattcat aatttttccg gctatcgtgc cctgacctcg ggtgcctttg
    12421 actggcgagt caatgacaaa ctctctttca agttcgacgt cgagcattat cgcaaggagg
    12481 tttcggaaca ggcagcgatc caggcgcccg ccgcggtcaa tggtgttatt accttgcccg
    12541 acgtgcccga taaccgcctg aatctcgccg gcgaatggca gaaatacgac gcggaagcga
    12601 ccaacctcct gcttcggggc gattatgcga tcaatgacaa ctggggactg ttattcgaag
    12661 tcggcaaggc gcgtactacg cgcgatcgta atttttcgca attccagaat tacaatctcg
    12721 tcacggggca ggggacgctg cgtacatttt tcaatcgcgg gcaggagttc gataatgaga
    12781 acgtcagggc ggaattattc ggccgcttgc cgggcaagtg gattacgcac gaggcatccg
    12841 tgggctttac gggaaacgag cgcagtcagg atcctcgtac atcctcatcg gttgatattc
    12901 cccaaaatct gtacaacccg atcgatatcg caaagaggtc tccaaccgtt cccttccgtt
    12961 caaattcgtc caagataacc gatttcggtt tctacctgtt tgatcgggtg ttcattggcg
    13021 acaagctgca ggccatgctg ggcgttcgcg gcagtttcta cgaaagtgtg gcggccacca
    13081 ctcacttcaa ggcggacagc gtcaatccag cgctctcgct gctatataag cctctcccca
    13141 acatttcgat atacggcagt tacctcgagg gggtcgagga aagcggccag gcaccggcta
    13201 atcgcgccaa tgccggccag ctattggcgc ctgcattgtc cacgcagaag gaaattggaa
    13261 tcaaagccgt cgttgcgcaa gggatattgt tgcaagtcgc agctttcgat atcaaacgcg
    13321 cttcaacgac agtggattcg gcgaatcggt tcgttctgaa cggaatggcg cagtatcggg
    13381 gtattgagtt ggcggcttcc ggtgaaatca caaagcagct gtcagtcatc gcctccgctc
    13441 tgttcatgga tgcgcggcaa ttgaacaggg cgaatgccga gaccttcgac cagatacccg
    13501 ataatacccc cgagcgtacc gccagtatgt tcgccgagta tcgtttgcaa agcatcccgg
    13561 ggctcgcgct cagcagcggc gtctattatg taggacggcg gccggtcaac aatgcaaacc
    13621 aggcattcgt ggatgactat gtgctgctgt ctctcggcgc ccgctatgcc tcccgcgtcg
    13681 cgggcaaacg caccacgatc caggcatttc tggacaatgc gacgaacgaa gattactgga
    13741 gtactgcggg aaacggcctc ctcggcgtcg gccctcccat tctgctgaag atcgcaggaa
    13801 gggtcgaatt ctgactatat cttgttccgg ttgcaggaga ttgaattttc agaggtgtgt
    13861 tttgcaggcg taagtctgta cccggcgtgc agggaacgtg atggccgggg atagattccg
    13921 ggtcaagccc ggaatgacga gtcgcggcgt tttttaagga gaagagccca gttgggtatt
    13981 ggtcatgaag atacgcagga tcgttttcaa ctttcacctt tatgccggtc tggcggcagg
    14041 gttgcttctc gtgttgagcg gattgactgg cagcatggtg gtttttcggg aagagataga
    14101 ggagctcatg tatccggaat tactggaaac tgtcgtccgg gacgagcagg ttcccataca
    14161 ggcagtgctg agcgcggcca gggaggctta tccgcgagac aagcttcttt ctatccgaat
    14221 gccgcgcgcg ccacagcaga cttatttact gaagatgaat ggccctcatg atttatttgt
    14281 atatgccgat ccctacactg gagcaatgct gggatcccac cggcaggagg ataccttcat
    14341 gggctggatc gctcttgtgc atacggagct tctgatcggt gagcgcggta aaaacattct
    14401 gggtctcagc gctttgctgt tagtctgcat gatcgttacc ggcttcttcc tctggtggcc
    14461 ccccggtgga aaggaaagaa atggaagggc aagaaaaatt ttgcggggct tcaagatcga
    14521 tttgtcaggg ccgtggaaaa agatgctctt tgatacgcac cgagcgatcg gtatctacac
    14581 ttcgattttt ctcctgattg tcgcctttac cggtgtttct ctggtgttca acaaagctgt
    14641 ggctgaactg gctaatttat tgaccgccag tcctccaagg ccagcgcctc ccgtctccat
    14701 tgctgtggca acaggtagag aaacgccttc actcgacgag ttgctgccca aggctgaccg
    14761 tgttctgccc ggtgccgtca cctggattaa ttttccgcaa acgccgcagg caccgcttgt
    14821 ggttcgaaag aaactgccgg aagaattgca tcctaacgga agaagtttcg tttattttga
    14881 ccagtactca ggcgaagtat tatttgtcga aaaggcgtcg gcgagcccta ttggctcgcg
    14941 catctacaat accttttacc caatccacac gggtgttata ggaggcgcat atactcgaat
    15001 cttacaggtc attgtcgggt tttcgccgct cgtcctgttg gttaccggtt atgtcatgtg
    15061 gagaaatcga aaaaaggcca aggcatataa agcgggatcg cggaaattgg gtttcaattc
    15121 agtagggctg gggcgggata agtgaggtat ttcccaggaa attactccgt cttctcgcgg
    15181 aagatacggc ttcaatgttc tatggaaatg gcctggaagg tcacaacaac agcactgaat
    15241 aatacatccg gtaaaccact tgcttgttga aaggcgctaa actgaaggag gcgattttta
    15301 acgattgaag ttatattcgc ttacgatacg caagtccaga tgccagccca gatgaagccc
    15361 taaatgtccg ataactcgtc aaaagcggaa gttaaattta gcatctgatt catttcctcc
    15421 cctgattcat tttcgtccgg gggttgtaaa accaggtctt aaagatcaga ctatctccgg
    15481 cgttgattat tcgtggtttg gcagcgggcg aggcggaaca agcggcatcg ttccgggcct
    15541 tctcacaccg gcggaccgcc gcgctctact gcactatatc gggcgaatcg tgaaatccag
    15601 catcctcacg ctttttttcc ttcttacctt ctccagcatt gcttctgcac aagctatccc
    15661 gcctcccgat ccaagaagcc ccccccggcc tcaggatgta cggatagcgc aattgcaaac
    15721 acgattaaac gccattaacc aggctcagca atccatgtac cagcagttcc agatgttgca
    15781 ggagatgcgc cggaatgaaa tcgcaggaac ttttccccag gtgctcccag ggggagggct
    15841 aagcaatctg ccgcccatca gttatgatga gaccgtcaga ttgcagcgcg aacggcagga
    15901 ccgcctgcaa cgatatgagc gcgatctcga cgagctgtat gcgcgatatg cagagctgga
    15961 tgaacagaag aaagctattc tcgatgaaat tgtggaactg gctaagcccg aggaatgagc
    16021 aggaacgtgc aatgaggagt ggctgccagt tccatgcaaa tattggaggc ggcagtgagt
    16081 acggtcaagg cgggaaatgt caagatgaaa cgcgcctatg aggcggctga gccgaccgat
    16141 ggtatccgga tactagttga ccggttatgg ccgcgtgggg tctccaagga tgaggctgcc
    16201 gttgaccaat ggatgaagga tgtggccccc agcactgaac tgcggaaatg gtttggacac
    16261 gatcccgacc gctgggacga attctgcaag cgctacgtgg ccgaactcga gcagcacccc
    16321 gatcaactca aggaactgcg tgagctggcg cgcaagagcc cgattacgct ggtgtattcc
    16381 gcccgtgacg aaacgcataa cgatgcggtc gtgctgagag attttatcct ccggcgagtg
    16441 aagtcggtcg accacgaata gagcgaaccg tcccccgttc gtttgtgcac gtaacaggtc
    16501 attattcaag aacaggtcgt tattcaagaa ttgctaaccg atcccttcgg cctgctattc
    16561 actgcttacc cgatgatttc tccggcaccc gcttccggat actcatcatt ttgagataag
    16621 caataatctc atccgccacc ttgtcgcggt taggaacctc gtgcccgagc gcgggcatca
    16681 tcgtgttgta ccggatggtc tgcggctgct cgatccatcg cttgaggtat tccggtttga
    16741 tgtactcgct tacactcacc ggataattga gttcgggcgc tttgccgccc ccctcgccat
    16801 tgattgtatg gcaggagacg cagtatttgc ggaaatgatc aaatccgcgg aggacctgct
    16861 tagatgagcc cttgggcgga gccaggccgg caaaaggatc ctccgacttg atctcgattg
    16921 tttttatttg gtagggcatg tcggatgcgc cggattcgag tagggctttc gagccgatgt
    16981 tgtcccatat caggtaaagc ggtccaagcg gcactttttc attgttttgc agcttgttga
    17041 gcatgatgaa tggagaacca tcgtcatgag caagtgcgag gtaggcgttg tgggccaaaa
    17101 acttctcgac cggaacgctg ggctgataac cgtcgatcga ggtgaaaacg atttgctggg
    17161 ccttttccca gttttttccg aatatccgtt cgaatacagg ccgggcggga attgcgcggt
    17221 agacgcgttc ctccttctca tgcgcctcga aaaccttcag cggcacgggg ggagcaatgg
    17281 cagcaaggtc atcgacagag aaagtcttga ccgtctggtt gtccttgcgg aactcaatcg
    17341 tgggcgcgct ttgtgcgagc acgcgaggga cctggagcgt gtaggcgaaa aggcaaatga
    17401 gccaagcagc aatacatgaa aacgtagctg taaatttcat attgttaccc atacttccca
    17461 ttcaaaggga tgacgtcact gcacgtttgc aaccggctcc agcgttattc cggctctgcc
    17521 acgccggcat caaattgcag ttgcatcgca gactgaggtt ttggccgctc aagataatag
    17581 ccctgaacca tatccacgcc gaggtctcgc accatgtcca gtgtcgccgc atcctcgacg
    17641 cattctgcga ccgtgatttt gcctaatccg cgcgcgactt ctaccatggc tttgacgaag
    17701 atctgattgt cgcggttgtc gggcaggttg catatgaaca gtccgtctat tttcaatatc
    17761 tgggcgttca aatacttgag atagccaaac gtggaaaaac cgctgccgaa gtcatcgagg
    17821 caaacccggc atcccgtgtg gccgatagct tcgatgaaac gttgcgcatc ctggatatct
    17881 gacaccgcag ctgtttcggt cagttcaatg ataaggcgat tcgggcttac attccattca
    17941 ttcaacag
//
LOCUS       DASSHJ010000074         5419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_320600, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000074 DASSHJ010000000
VERSION     DASSHJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320600"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFI43_03045"
     CDS             <1..304
                     /locus_tag="VFI43_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HET7061135.1"
                     /translation="AAIDDPRRADKAGAMIALVGVVNVPIIYFSVKWWNTLHQGASVS
                     LSQSPKMATTMLTGMLIMSIACWMYAIAVILVRTRAIILERERHTAWVGQLQGAVQ"
     gene            301..498
                     /gene="ccmD"
                     /locus_tag="VFI43_03050"
     CDS             301..498
                     /gene="ccmD"
                     /locus_tag="VFI43_03050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HET7061136.1"
                     /translation="MNWTSVSDFLDMGGYGLYVWGSYLVFLVCIAGEVILVFNRKRTL
                     LKHLSLIHQSTKQEKRHETAS"
     gene            482..931
                     /gene="ccmE"
                     /locus_tag="VFI43_03055"
     CDS             482..931
                     /gene="ccmE"
                     /locus_tag="VFI43_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET7061137.1"
                     /translation="MKPRHKKLAIIASSVTALGVASVLVLNAFQSNLVFFFSPTQVAN
                     KEAPIGKSFRIGGLVEEGSVKRERDGVTVNFVVTDTAKVIPVVYKGILPDLFKEGKGV
                     VAQGKLASDGVFRADEVLAKHDENYMPPEAAGAIDQADKARKTVLVQ"
     gene            1033..3063
                     /locus_tag="VFI43_03060"
     CDS             1033..3063
                     /locus_tag="VFI43_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET7061138.1"
                     /translation="MIPELGNFALILALLLALIQGTLPIIGAARGIPSWIALARPVVQ
                     GQFVFVIIAFFCLAYSFATSDFSVINVVQNSNSKLPLEYRLAATWGSHEGSLLLWTTM
                     LATWSVAVSIFSRHLPDDMVARVLGVMGLVSVGFLIFMLFTSNPFERMLPGAMEGSDL
                     NPLLQDPGMVFHPPMLYMGYVGFSVAFAFAISALLSGQMDATWARWSRPWTTVAWLFL
                     TIGIMMGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALVHSLAVTEKRGSFKSWTV
                     LLAICAFSLSLLGTFLVRSGVLTSVHAFATDPKRGIFILTFLVIICGGSLLLFAWRAP
                     KIGLGGKFDVVSRESMLLANNLLMMVAAGSILLGTLYPLFMDALELGKISVGPPYFEA
                     VFVPLMTPAIFLIGLGPLARWKQASIPELAVRLRWAFAVSVITALLMPFVMGEWKPFV
                     SFGLLLAFWVIASIVTSLVHRVQSSGKQGLFAKLSAQSRSYYGMHAAHLGIAVFIIGV
                     TLVGGYETEKDVRMDVGDTVEVGGYTFRFNGATRGPGPNYVADIGHVDVLRNGEKMST
                     LEPEKRTYNASGMAMTEAAIDTGFLRDLYVALGEPLENGAWIVRVYHKPFVDWIWFGC
                     MLMAFGGFLAVTDRRYRLSSRKQHEQVEDKPVVSKPRRSPAAPVIARSKKGA"
     gene            3060..3584
                     /locus_tag="VFI43_03065"
     CDS             3060..3584
                     /locus_tag="VFI43_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET7061139.1"
                     /translation="MMRFLVPLAIFVVLVGFLVVGLGLKPREVPSPLIGKPAPEFQLR
                     QLHDSDKMLTSKENLGKVWLLNVWASWCVSCRQEHPLLVELAKSGIVPVYGLNYKDQR
                     DDALRWLKQFGNPYTISIVDPEGRTGLDYGVYGVPETYVIDKNGVIRYKQIGPVTVDA
                     LQTKILPLVKELQG"
     gene            3652..4122
                     /locus_tag="VFI43_03070"
     CDS             3652..4122
                     /locus_tag="VFI43_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET7061140.1"
                     /translation="MKKFGLLLALMLLLPVSWAKEAAPVAEDPELERRMVTLSEELRC
                     LVCQNETIAASRADFANDVRREIREQMKADKSDKEIVDFLVARYGDFVLYRPPVKSST
                     FFLWFGPFIFLVIGAIVLFVYLKRRRKQIEEPMLTEQQLKQAESLLKEGKGNNA"
     gene            4119..>5419
                     /gene="ccmI"
                     /locus_tag="VFI43_03075"
     CDS             4119..>5419
                     /gene="ccmI"
                     /locus_tag="VFI43_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET7061141.1"
                     /translation="MTTFWVVAGIFIVGALLFVLPTLWSKRERRSGVARDATNIDVYR
                     DQLAELDNDLRSDVLTREQYEQSKRELQQRMLQDVPFAGAGAGGGGVLPSGMLSSGSN
                     AATITLTTLALPVLAVSLYLWLGNTRALLPQRAMEQAAMEQAPMSAEGGHPDFSSVLQ
                     NLVARLKDQPDDMEGWLMLGRTYAMMQRFNEAKEAYEKVLALAPENPEVLTDYADIVA
                     MTNNGSLMGQPLELVNKALRIDPQNPKALALAGTAEFERRRYKEAAGYWEKLLALIPP
                     SETELVQSVNNSIAEAKSLAAGKGSTVARAGQSPGGQMPPPAEKPASVAAAGAQGSGT
                     LSGKVTLSPAMASKVSPADSVYVFARAKTGPRAPLATLRLSVKDLPATFTLNDSMARS
                     GVQLSTFPNEVIVGARISKSGSPMPQSGDLQGLSQPVSVGAS"
BASE COUNT         1128 a         1498 c         1578 g         1215 t
ORIGIN      
        1 ggcggcgatc gatgatccac gccgggctga caaggctggc gcaatgatcg ccctggtcgg
       61 ggtggtgaac gttcccatca tttatttctc ggtcaaatgg tggaatacct tgcatcaggg
      121 tgcctcagtc agcctgtccc aatcgccgaa gatggcgaca acgatgctga ccggcatgct
      181 gatcatgtcg attgcatgct ggatgtacgc gatcgccgtg atactggttc gcacccgcgc
      241 catcatcctt gagcgcgaac ggcatacagc gtgggtaggc cagttgcagg gggcagtgca
      301 atgaactgga cgagcgtgtc cgattttctc gatatggggg gatacgggct ctacgtgtgg
      361 ggctcatacc tggtctttct cgtatgcatc gcgggagaag tcattctggt cttcaaccgt
      421 aagcgaactt tgctcaagca tctcagtctg attcaccagt caaccaaaca agagaagaga
      481 catgaaaccg cgtcataaaa agctggcgat tatcgcttcg agtgtcaccg cactgggcgt
      541 tgcttctgta ctggttttaa atgcctttca aagtaatctc gtttttttct tcagccctac
      601 ccaggtggca aataaagaag cgccgattgg caaaagtttc cggatcggcg gtctagtgga
      661 agaaggcagc gtcaagcgcg aaagagacgg ggtaactgtc aactttgtcg tgacggatac
      721 tgcaaaggtc ataccagtcg tatacaaagg catccttccc gatctcttca aggaaggcaa
      781 gggtgtggta gctcaaggaa aacttgcttc tgacggggtt ttccgcgcgg acgaggtact
      841 ggcgaaacac gacgaaaatt acatgccgcc ggaagcggct ggcgccatcg accaggcgga
      901 caaggcgcga aaaacggtcc tggttcaata acaaacagtg tgtggcctga aagacaggga
      961 tcggaaagtc ttccccaacc cgaatcctgg gccctgcggt ccttttctca ctcctaatcg
     1021 atagccccga ccatgattcc agaacttggt aatttcgctc tgatcctcgc gttgctgctg
     1081 gcgctcattc aaggaacgct gcccattatc ggggcagcac gcggcatccc ttcctggatc
     1141 gcgctggccc gcccggtggt acagggacag ttcgtattcg tcatcatcgc cttcttctgc
     1201 ctcgcctatt cttttgcaac gagtgatttc agcgttataa acgtagtcca gaactccaat
     1261 tccaagctgc ccctggaata ccgtctcgcg gcaacctggg gctctcacga aggctcactg
     1321 ttgctgtgga cgacgatgct tgcgacctgg tctgtggccg tgagcatatt cagccgccat
     1381 ctgcccgatg acatggtggc ccgggtgctg ggagtcatgg gccttgtcag cgttggcttc
     1441 ctgatattca tgcttttcac atccaacccg ttcgagcgga tgctgccagg agcaatggag
     1501 ggaagcgatc tgaaccccct gctccaggac cccggaatgg tattccaccc ccccatgctg
     1561 tacatgggct acgtgggctt ttcggtcgcg tttgcctttg cgatttcggc gttgctcagc
     1621 ggccagatgg atgcgacctg ggcgcgctgg tcgcgtccct ggacaaccgt ggcctggctg
     1681 ttcctgacga tcggcatcat gatgggaagc tggtgggcct attacgagct cggctggggc
     1741 ggctggtggt tctgggaccc ggtggaaaac gcctcgttca tgccatggct cgtgggtacc
     1801 gccctggtcc attcgcttgc cgtgaccgaa aaacgcggca gcttcaaaag ctggacagtg
     1861 ctgctggcaa tctgtgcgtt ctcgctgagc cttcttggca ccttcctcgt ccgctcgggc
     1921 gtgttgacat cggtgcatgc cttcgcgacc gatcccaagc gcggaatttt catcctcacc
     1981 ttcctcgtga tcatctgtgg aggctccctc cttctattcg cgtggcgcgc gcccaagata
     2041 ggcttgggcg gcaagtttga tgtggtgtcg cgggaatcca tgctgcttgc caacaacctc
     2101 ctgatgatgg tagcggcggg cagcattctg ctgggcactc tctatccgct tttcatggat
     2161 gcgcttgaac ttggcaagat atccgtcggc cccccctact tcgaagccgt tttcgtgcct
     2221 ttgatgactc ccgccatttt cctgataggg cttgggccgc tcgcccggtg gaagcaggca
     2281 agcataccag agctggccgt gcgtctgcga tgggcgttcg cggtgagcgt gataaccgcg
     2341 ctcctgatgc cttttgtcat gggagaatgg aaacctttcg tgagctttgg cctccttctg
     2401 gctttctggg tgattgccag cattgtgacc agtctcgtcc accgtgtgca aagcagtggc
     2461 aagcagggcc tgttcgccaa gctgagtgcg caatcgcgaa gctactacgg gatgcacgct
     2521 gctcacctgg gcatcgcggt attcattatc ggcgtgacgc tggtgggcgg ctatgagacg
     2581 gaaaaggacg tgcgcatgga cgttggcgac acggtcgagg taggcggcta tacgttccgt
     2641 ttcaacggcg cgacaagagg gccggggccc aattacgtgg cggatatcgg acatgtggac
     2701 gtgcttcgca acggtgaaaa aatgagtacg ctcgagcccg agaaacggac gtacaacgcg
     2761 tctggaatgg ccatgaccga agcggcgatc gacacgggct tcctgcgcga cctttacgtt
     2821 gcgctgggcg aacccctgga gaatggcgcc tggatcgtgc gggtgtatca caagccgttc
     2881 gtggactgga tctggttcgg atgcatgctt atggcatttg gaggtttcct cgcggtaacc
     2941 gaccggcgct accggctgtc gtcgcgcaag caacatgagc aggtggaaga caaaccggtt
     3001 gtcagcaagc cccgcagatc ccctgcagca cctgtgatcg cgaggagtaa gaagggggca
     3061 tgatgcggtt cctggttcca ctggcaattt ttgtcgttct cgtaggtttt ctggtagtgg
     3121 ggctcgggct caagcccaga gaagtccctt ccccgctaat cggaaaacct gcgccggaat
     3181 tccagcttcg gcaattgcat gattctgaca agatgctgac ctcgaaggaa aacctgggca
     3241 aggtctggct attgaacgta tgggcttcct ggtgcgtttc ctgccgtcag gagcacccgc
     3301 tgcttgtgga actcgcaaaa tccggaatcg ttcctgttta tggactcaac tacaaggacc
     3361 agcgtgacga tgctttgcgc tggctgaagc agtttggcaa cccgtacacc atcagcattg
     3421 tcgatcccga agggcgtact ggactcgatt atggcgtgta cggcgttccc gagacttatg
     3481 tgatcgacaa aaacggcgtc atccggtaca agcagatcgg ccctgtcacg gttgatgcgc
     3541 tccagacaaa aatcctgcct ctggtaaaag agttgcaggg ataacccctt cttcgtccag
     3601 actctctctg tgtagccata tacacagcat ttaacgaatc actctttcag catgaaaaaa
     3661 ttcggattat tgctggcttt gatgcttttg ctgcctgtct cgtgggcaaa ggaggctgct
     3721 ccggtcgctg aggatcccga gcttgaacgg cgcatggtca ccctctcgga ggagttgcgc
     3781 tgccttgttt gccagaatga aaccatcgca gcgtcacgcg cggattttgc caatgatgtg
     3841 cggcgggaaa ttcgcgaaca gatgaaagca gataaaagcg ataaggaaat cgtcgacttt
     3901 ctggtcgccc gctatggcga ttttgtgctc taccgccccc cggtcaagtc cagcaccttc
     3961 ttcctgtggt ttggtccttt cattttcctt gtaatcgggg caatcgtact cttcgtttac
     4021 ctcaagcgcc gccgcaagca gattgaagag ccgatgctca ctgaacagca actcaagcaa
     4081 gctgaatccc ttctgaagga aggcaaaggt aataacgcat gacgacattc tgggttgtcg
     4141 ccggcatatt cattgtcggc gcactgttat ttgttctccc tacgttgtgg agtaaacggg
     4201 agcgcaggtc aggggttgcc cgtgacgcaa ccaatatcga tgtttatagg gatcagcttg
     4261 ctgaactcga taacgacttg cgcagcgacg ttctgacgcg agagcagtat gagcagagca
     4321 aacgcgaatt gcagcagcgc atgttgcagg acgtcccgtt cgcgggagca ggggcaggag
     4381 gaggcggtgt ccttccttcc ggtatgctga gcagcggcag caatgcggca actatcacac
     4441 tgactaccct agcgctaccg gtactggcgg tttcccttta tctctggctg ggcaatacca
     4501 gggccttgct gccgcaacga gccatggaac aggcagccat ggaacaggct ccgatgtcgg
     4561 cagagggcgg tcatcctgat ttttcttccg tactgcaaaa cctcgttgca cgcctgaagg
     4621 accagcctga cgacatggaa ggctggctca tgctgggacg aacctacgcc atgatgcaac
     4681 gtttcaacga ggcaaaggaa gcctatgaaa aagttctggc gctcgctcca gaaaatcctg
     4741 aagtgctcac ggattacgcc gacattgtcg cgatgacgaa caacggcagt ctgatggggc
     4801 agccccttga gctggtcaac aaggcgctgc gtatcgatcc gcaaaatccc aaggcgctgg
     4861 ctctggcagg aacggcagag ttcgagcgca ggagatacaa ggaggccgcc ggctactggg
     4921 aaaagttact ggcgcttatt ccaccatcag agacggaact ggttcagtcc gtgaataaca
     4981 gcattgccga agccaagtcg cttgctgcgg gcaaaggcag tacagtcgct cgggcagggc
     5041 agtcgccggg cggacagatg ccacccccag ccgaaaaacc tgcctccgtt gctgcggcag
     5101 gcgcgcaggg atcaggcacg ctatcgggca aggtgacgct gagtcctgcc atggcaagca
     5161 aagtctcccc cgcagacagc gtctatgtgt ttgcgcgcgc caagactggg ccgcgggccc
     5221 cattggcaac actccgccta tccgtcaagg atttaccagc cacgttcaca ttgaacgact
     5281 cgatggcaag gtcgggggtg cagctctcca ccttccccaa tgaggtcatc gtgggcgcca
     5341 ggatatcaaa atcgggctct cccatgcccc agagtggtga ccttcaaggc ctcagccagc
     5401 ccgtcagcgt aggcgcctc
//
LOCUS       DASSHJ010000075         6671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1153245,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000075 DASSHJ010000000
VERSION     DASSHJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153245"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFI43_03080"
     CDS             complement(<1..812)
                     /locus_tag="VFI43_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET7061142.1"
                     /translation="MLLVTLSVSFALTQIIPGASAGSVGPPVSPQPPLTESSSMNVTV
                     GKSALLRLPAPIARLSVGNPDIADVALINPAQIYVLGKTIGTTNVIIWTRDSQTSVID
                     VTVTIDAPALQRKLEELMPGESRVKVSAAGDSLVLSGTVSDTLKADRIVSLADAYIRA
                     ATGAKGSSQRPTAQATAQGSGPDNNQAGAQGGSEGAGQGLGSTRVINLLQIGSNQQVM
                     LEVKVAEINRTVAEKLGFDFQRAARKAGSAWTQIMTGVIGGAPAQLFQSLGNP"
     gene            complement(923..1744)
                     /gene="cpaB"
                     /locus_tag="VFI43_03085"
     CDS             complement(923..1744)
                     /gene="cpaB"
                     /locus_tag="VFI43_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET7061143.1"
                     /translation="MKNTRAIWMIAISLLLGLAAVLVAGKWVIQRTTVEGSRVVIAAQ
                     DIDVGTRLTPDLLQSADWPRASVPPGSFQETKMLDSRVAKVNLVRGEPLLESKLAPVG
                     AMGVLSGVIADGKRAITVKVNEVVGLAGLALPGNKVDILVNTKDESDKPISKIVLEQI
                     LVLAVGQDLGRDETKPKTVSAVTLEVTPEEAEKLDLARSVGNLSLVLRNQVDKSPGTT
                     AGVRASDLLKLAPPTAAAPVIAPAIRRKAVPPEETVEIIRGIDKTEASLGAKGSS"
     gene            complement(1915..2463)
                     /locus_tag="VFI43_03090"
     CDS             complement(1915..2463)
                     /locus_tag="VFI43_03090"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET7061144.1"
                     /translation="MYPLTIGILLLLLLVAALHDVRTRRIPNVLVLTGVIAGLSFNSL
                     LPKGMGGLGAFASLAGVAAGLLLLLPLYLFRAMGAGDVKLMSMIGAFLGVAEVMHVFI
                     YVLLAGGLLAMGTIWARRKPQAIPVKLRKMRVAGTLPIGPVSRPEGLEDSASAPAKTV
                     TLPYGVAIALGTAWYLVAVPLL"
     gene            complement(2589..2780)
                     /locus_tag="VFI43_03095"
     CDS             complement(2589..2780)
                     /locus_tag="VFI43_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET7061145.1"
                     /translation="MNMIVQYVKQFLSEEDGVTAIEYGLIAALIAVVIIGGVQLAGTN
                     LQGVFSFVAGKLTTGGGGS"
     gene            3095..4420
                     /gene="bioA"
                     /locus_tag="VFI43_03100"
     CDS             3095..4420
                     /gene="bioA"
                     /locus_tag="VFI43_03100"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET7061146.1"
                     /translation="MDTRSTKHESLPQRSLKAVWHPCTQMKQHEKLPPIPIARAEGVW
                     LYGPDGQGYLDAIGSWWVNLFGHSNPRINAAIRQQLDKMEHTMLAGFTHEAVVELSER
                     LSSLISGKLGHCLYSSDGASAVEIALKMSFHYWRNCGRPDKANFVSLENGYHGETLGA
                     LSVTNVELFAEAYAPLLRPSTQVRTPDWRFAADSESPEEYALRAAGVLEAHLAQHHTR
                     TAAFIVEPLVQGATGMGMYHPVYLTHAREICDRYEVHLIADEIAVGFGRTGTMFACEQ
                     AGIAPDILCLGKGLSGGYLPLSTVMATDEIYQAFYDDDITRGFLHSHTHSGNALACRA
                     ALATLDIFDDGDIITANRAKADYLNQSLLPLADHLRVKNFRNCGMIWAFEVETGDPAF
                     SLNFFQAGLREGLSLRPLGNTVYFMPPYIIDQGEMDHLAAGSLRALESC"
     gene            4466..6031
                     /gene="dacB"
                     /locus_tag="VFI43_03105"
     CDS             4466..6031
                     /gene="dacB"
                     /locus_tag="VFI43_03105"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET7061147.1"
                     /translation="MINIALPKSSYTGLVLPLLLLPLFLPVAPAFSRDLPAPVHRTLR
                     QAGILESAVSVYIHEIGTSQPLLQHNAGIPMNPASVMKLVTTYAGLELLGPAYTWKTE
                     LYADGLRDGDTLRGNLIIKGYGDPKLNLENFWLLTRRLRQTGLREIAGDLILDTNYFQ
                     LPSVNPAEFDGKPHRAYNVLPEALLVNYRAVSLRLIPTPENGNVRVIVDPLPALLDLR
                     NSLTLTPGACGDWRDALATDIRANRENDGENHGRVSIALNGTYATDCGEKNLFLSVHE
                     NAYYIQSLFKQLWEEQGGTFHGRGIPGTVPDGLLPIEIHQSPPLADIVRDINKFSNNT
                     AARQLYLALGVAEPVVESTAIVRATTLNPAVAGGQASSEPMAPATLAKADASIRRWLA
                     SKRLHFPELVMENGSGLSRNERISARHVGQLLLTAFQSPVMPEFISSLPIAAVDGTMK
                     KRLNGSATAGQAHIKTGSLEGVQTMAGYMRDQSGKRLVVVFFVNHPNAGRAQAAMDML
                     LHWLYEQSVRSRG"
     gene            complement(6124..>6671)
                     /locus_tag="VFI43_03110"
     CDS             complement(6124..>6671)
                     /locus_tag="VFI43_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET7061148.1"
                     /translation="GVSNEQRRYAKVINFGLIYGMSEFGLASQLGVDRSAARAYMDRY
                     FARYPGVADYMQRTRQMARQSGYVETVFGRRLWLPEINSSNVNRRQASERAAINAPMQ
                     GTAADIIKLAMIAVNSWLKINGLRSKLIMQVHDELVLEVPDEELEKVISELPSIMTAV
                     TCLDVPLLVEVGVGPNWEKAH"
BASE COUNT         1478 a         1942 c         1789 g         1462 t
ORIGIN      
        1 ggatttccca gggactggaa cagttgcgct ggtgcaccgc ctataacccc tgtcatgatc
       61 tgcgtccagg cgctcccggc tttccgggca gcacgctgga agtcgaaacc aagcttttca
      121 gccacggtac ggttgatttc agctaccttc acttccagca tcacctgctg gttgctgccg
      181 atctggagca ggttgatgac cctcgtgctt ccaagcccct gacccgcgcc ctcggaacct
      241 ccctgtgcgc ctgcctgatt attgtccggg ccactacctt gtgcggttgc ctgcgcggtc
      301 gggcgctggc tgcttccctt tgctcccgtt gctgcacgta tgtaggcgtc ggccagcgag
      361 acaatgcggt ctgccttgag cgtatcggac accgttcccg acagtaccag cgaatctcct
      421 gccgcgctga ccttgacacg cgactctcca ggcatcaatt cttccaattt tctctgtagt
      481 gcgggcgcgt cgattgttac ggttacgtct atcaccgagg tctggctgtc ccgcgtccag
      541 atgatgacgt tggttgtccc gatagttttg cccaacacgt atatctgcgc cggattgatg
      601 agggcgacgt cagcaatatc cggattgccg acggacagac gtgcgatcgg cgccggtaac
      661 cggagcagcg ctgacttgcc caccgtcaca ttcatgcttg aggattcggt cagcggcggc
      721 tgcggcgaaa cgggaggacc aaccgaaccg gcggatgcgc cgggaatgat ctgggtcaac
      781 gcgaagctca cggaaagggt tacaagcaac aagatggatc ggagtttgcg aatacacgca
      841 aacgcgtgtg ggttcggggt tatcacaagt gataaccttc cagcgcgatt tgcaggaaga
      901 ccgttaagca gccggcgcgg gatcatgagg atcctttcgc tccgaggctg gcttcggttt
      961 tatcgattcc cctgataatt tcaacggtct cctcgggcgg gaccgctttc ctgcgaatcg
     1021 caggcgcaat cacaggcgct gctgcggttg gaggagccag tttcagcaga tcgctggccc
     1081 gaacacccgc cgtcgtaccc gggctcttgt ccacctggtt gcgcagcacg agggacaggt
     1141 ttcccacgct gcgcgccagg tcaagctttt ccgcctcctc cggagtcacc tccagggtta
     1201 cggcagacac ggtcttcggc tttgtttcat cgcgcccaag atcctgaccc acggccagca
     1261 ccaaaatctg ttccaggaca atcttcgaga tgggcttatc gctttcgtcc ttcgtgttta
     1321 ccaggatgtc caccttattt cccggcaagg ccaatcctgc caggccaaca acctcgttca
     1381 ctttcaccgt aatggcgcgc ttgccatccg caatgacacc tgataataca cccattgcgc
     1441 ccaccggagc cagcttcgat tccagcagtg gttcgcctcg caccagatta accttcgcaa
     1501 cgcgagagtc gagcatcttc gtttcctgga aggatccggg gggcacgctg gcgcgaggcc
     1561 aatctgccga ttgcaacagg tcgggcgtga gccgggttcc tacgtcgata tcctgggcag
     1621 cgatcaccac cctggatccc tctacggtcg tacgctggat cacccatttt ccggcaacca
     1681 gtaccgcagc cagccccagc aacagggaaa tcgcgatcat ccatattgcc ctggtgtttt
     1741 tcattctgct tccttttacc cttacagctc gtcgttatat caaccgctct ttataatcct
     1801 ttattccggg tacacgcttt ccaagcagaa atgaaaaatg actgctttta gcggcttaga
     1861 aagaagttcg agaaaaaaag ccaaggaaag caaagtccgc ttcccggcgg ccagtcagag
     1921 gagagggaca gcaaccaggt accatgcggt accgagggcg atggctacac cgtagggcaa
     1981 ggtgactgtt tttgccggcg cacttgcgga atcttctaaa ccttcgggtc gagatacggg
     2041 tcctatgggg agggttcccg caacccgcat tttcctgagc ttgaccggga tcgcttgagg
     2101 cttgcgccga gcccagatcg ttcccatcgc gagcaagccc cccgcgagaa gaacatagat
     2161 gaatacgtgc attacctcgg ctacgcctag aaaagcccca atcatggaca tgagcttgac
     2221 gtcgcccgcg cccatcgcac gaaacagata gagcggcagc agcaacagca ggcctgcagc
     2281 cacgcccgcc agggaagcga aagctcccag tcctcccatt cccttcggca gcagagaatt
     2341 gaatgacaag ccggcgatga caccggttag caccagcaca ttcgggatac gacgggtacg
     2401 aacatcatgc agggctgcaa caagaagcag caacagcagg attccaatgg tcaacggata
     2461 cactgcatca ctcggccaag gcgggatgag aatgcagcgg cgcgtataag acttcgagaa
     2521 cgttatcagg actgccaagg gtggtcagaa aggaccatac cctggcattt tgatgaagcg
     2581 cccttcgttt aacttccacc acctccggtc gtcagcttgc ctgcaacaaa gctgaaaacg
     2641 ccctgaaggt tggtgccagc aagctgtacc ccgccgatga tcaccactgc aatcagggcc
     2701 gcgatcagac cgtattcgat cgcggtcacg ccatcttctt cgctcagaaa ctgttttacg
     2761 tattgcacaa tcatattcat aactttctcc cgttcgttga gttcaccgat taaataatcc
     2821 aatcaatgcg cgagactcat caaaaacctc ttgtcactct tgatgggtcc ttcacgtttg
     2881 cttaacggcg agcactttgg gaaacttgaa cagatttttg ggtacggaaa taacgctttc
     2941 ttcccgattc gggacgctat cgatggcagc cctattcagg aagccaacta cgtccggata
     3001 atcggcgccg attacgtgca ataatgcgac ggcgcaaaca ggcgaagacc cctcacgact
     3061 gatgcattcg tttaaacgct tcgggaaccg cagaatggat actcgctcga cgaaacacga
     3121 atccctcccg caacgcagcc tgaaggcagt ctggcatcca tgcacgcaga tgaagcagca
     3181 cgaaaaactg ccgccgatac ccattgcccg ggctgagggg gtgtggctct acggccctga
     3241 cggacaaggc tacctggatg caatcggttc gtggtgggtg aatcttttcg gtcattcaaa
     3301 cccacgcatc aatgcagcga tccgccagca actggataag atggaacaca ccatgctggc
     3361 cgggtttacg cacgaagccg tggtggagtt atccgagcgc ctcagcagtc tgatatcggg
     3421 caaactcggc cattgcttgt acagcagcga cggtgcatcc gcagtggaaa tcgccttgaa
     3481 gatgagcttc cattactggc gaaactgtgg ccgccccgac aaagccaatt tcgtgagcct
     3541 tgagaatggc taccacggcg aaacgctggg agcgctgtca gtcaccaacg tggaactgtt
     3601 tgcggaggct tatgccccgc tgctgcgccc cagcacacag gtcaggacgc ctgattggcg
     3661 ctttgcagcc gacagtgaat ctccggagga gtatgcgttg cgcgcggcgg gtgtgctcga
     3721 agcccatctt gcgcaacacc acacccggac agccgccttc atcgtcgagc cgcttgtaca
     3781 gggggcaacg ggtatgggaa tgtaccaccc ggtttatttg acacacgcaa gggaaatctg
     3841 cgaccggtat gaggttcacc tgatcgcgga tgaaattgcc gtgggtttcg ggagaacggg
     3901 caccatgttt gcctgcgaac aagctggcat tgcgcctgac atcctgtgcc tgggcaaggg
     3961 tttgagtgga ggctaccttc ccctctcgac cgtgatggcg accgatgaaa tttaccaggc
     4021 attttacgat gatgatatta cgcgcggttt cctgcattct catactcatt cgggtaatgc
     4081 ccttgcctgc cgggccgcgc ttgccacgct ggatattttc gatgacggcg atatcattac
     4141 cgctaatcgt gccaaggcgg attacctcaa tcagtcattg cttccgttgg ctgatcacct
     4201 tcgcgtaaaa aatttccgta actgcggcat gatatgggct ttcgaggtgg aaacaggcga
     4261 tcccgctttt tcgttgaatt tctttcaagc gggccttcgg gaaggcctgt ctttacgccc
     4321 gttaggaaat actgtttatt tcatgccgcc ctatatcatc gatcaggggg aaatggatca
     4381 tcttgccgcg ggcagcttac gggcactgga gtcttgctaa ataagtacgc gagccatgga
     4441 attcacgaat tcattggaac ggcgagtgat taacattgct ctccccaagt cgtcatatac
     4501 gggattggtc ctgcccttgc ttcttctacc cttgtttctt ccggtggcac ctgcattctc
     4561 gcgcgacttg ccggcgcctg tgcaccgaac ccttaggcag gccggcatcc tggagtcggc
     4621 agtgagcgtc tatattcacg agattgggac atcccagcct ctgcttcagc ataatgccgg
     4681 cattccaatg aaccctgcat ccgtcatgaa gcttgtcacg acctatgcgg ggctcgaact
     4741 gcttgggcca gcttatacct ggaagactga attgtacgca gatggtctgc gcgacgggga
     4801 tacgcttcgc ggcaacttga tcatcaaggg gtatggcgat cccaagctga acctggagaa
     4861 tttctggctg cttacgcgcc gcctgcggca gacgggactg cgcgagatcg ctggcgacct
     4921 catcctcgat accaattatt ttcaactccc cagcgtaaat ccggcggaat tcgacggcaa
     4981 gccccatcgt gcttataacg ttctaccgga agcattactg gtgaattacc gggctgtcag
     5041 cctacgcctc atcccaacac ctgaaaacgg gaacgtgcgt gtcatcgttg acccgctgcc
     5101 tgcattactc gatctcagga acagtttgac actgactccc ggagcatgcg gcgactggcg
     5161 cgatgcgctt gccacggata tacgcgccaa cagggagaac gatggggaga accatggccg
     5221 agtatcgatt gctcttaatg gaacctacgc cactgattgc ggcgaaaaga acctgttcct
     5281 gagcgttcac gaaaacgcct attacataca aagccttttt aaacagctgt gggaagaaca
     5341 gggggggact tttcatggca gaggaatccc ggggacggtg cccgacggac tgcttccgat
     5401 cgaaattcat cagtcgccac cgcttgccga tatcgttcgc gacatcaata aattcagcaa
     5461 caatacagct gcgcggcaac tctatcttgc gctcggggtg gctgaaccag tggtcgaatc
     5521 tacggcaata gtgcgggcga ccacgctgaa cccagcggtg gcagggggcc aggcaagcag
     5581 cgaaccaatg gcacccgcga cgcttgccaa agcggatgct tccatccgac gatggcttgc
     5641 ttcaaaaagg cttcattttc cggaactcgt catggagaac ggttctggcc tatcgagaaa
     5701 tgagcgcatc agcgcacgcc atgtcgggca acttctcctc accgcgttcc agagcccggt
     5761 catgcccgag tttatatcct ccctccctat cgcagcggtg gatgggacca tgaaaaaaag
     5821 gctcaacggc agtgccacgg cgggtcaggc ccatatcaag acgggctccc tggagggagt
     5881 gcaaacgatg gccggatata tgcgcgatca atcgggcaag cgcctcgtcg tggtattttt
     5941 cgtcaatcat ccgaatgcag gaagagcgca agctgcgatg gatatgctac tgcactggct
     6001 ttacgaacaa tcggtcagat cgcgaggata aagctcgtaa aagtcaaagc gcgacatcgg
     6061 ggaacaacat cgctttgccg gcccttgaag ttttgcaagg ccttgaccgt cgagctgccg
     6121 cgttcaatgc gctttctccc agttggggcc tacgcccacc tccaccagca gcggaacgtc
     6181 gaggcaagtc accgcagtca taatggatgg cagttcggag attacttttt ccagttcctc
     6241 gtcagggact tccagcacca actcatcatg aacttgcatg atgagcttgc tccggaggcc
     6301 gttgatcttg agccatgaat tcactgcaat cattgcaagc ttgatgatgt ctgccgcagt
     6361 tccctgcata ggtgcgttta tcgctgcacg ctccgacgct tgccttcgat tcacattgga
     6421 gctattgatc tccggcagcc atagcctccg cccaaacacg gtttccacgt aaccgctctg
     6481 cctcgccatt tgccgggtgc gctgcatgta atctgcaaca ccgggataac gggcaaaata
     6541 acgatccata taagctcgcg cagcagagcg gtcgaccccg agctgggaag caagaccgaa
     6601 ctccgacatg ccgtagatca acccgaaatt tatgaccttt gcataacggc gctgctcatt
     6661 gcttactcct t
//
LOCUS       DASSHJ010000076        24317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1279357,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000076 DASSHJ010000000
VERSION     DASSHJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 24317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24317
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279357"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFI43_03115"
     CDS             complement(<1..455)
                     /locus_tag="VFI43_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061149.1"
                     /translation="MPASLFPSIDLTTGCILTGMAIVAFSGSVSARENSSRELVVTNR
                     YTIPLLEISGLASAPALDSKGAQTVNPSLRLYAVGDATREVVTFYIDGSSGGIVISVK
                     NAALPSGRRAVSASQWEAVAADGVDTVCMLSETDSKVSCFDDRIDENRG"
     gene            complement(679..1107)
                     /locus_tag="VFI43_03120"
     CDS             complement(679..1107)
                     /locus_tag="VFI43_03120"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7061150.1"
                     /translation="MTTRKGWAVWKGGIKDGSGTISTETGVLHDAPYGFKSRFEKGEG
                     TNPEELLAAAHAACFSMALSLMLGEAGMTPERIDTHADVTIEKVGEGFEITQSHLNVS
                     AKVPGADDTKFQEIAHKAKSGCPVSKLFKADITMEARLEH"
     gene            complement(1104..2978)
                     /gene="nhaA"
                     /locus_tag="VFI43_03125"
     CDS             complement(1104..2978)
                     /gene="nhaA"
                     /locus_tag="VFI43_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET7061151.1"
                     /translation="MTPESPTNKLDRPVDNTYDHVLGPADAEITLVEYGSYADPASRL
                     AHDRIAQMRNRFGNRLRYVFRHRPLAGNQIARQAAEMVESSDDPSRFWRMHVALIGRS
                     DELTEEDLRKVAAEFQLGDVSPAAALLAKTRVDADIESAAASGAIVTPTFFINERRYD
                     GPWDANSLSEAMLGSLGHVVGAAALDFAKWAPSTGVLLLITTGMAVILSNSPLGPSFN
                     YLWQQPVGLSFGTAAFSMPLRQWINDGLLVVFFLVVGLEIKREFTVGHLASLQAAMFP
                     IVAAIGGMAVPALLYLLWVPAGPEGEWLRGWGVPTATDTAFAVALIAMMGNRVPIELR
                     VFLTAAAIVDDIGAIIIVALFYSGELHLAYLGAAAGIVGLLAVLNKGGVYRATPYVLL
                     GIGLWICVHASGIHSTLSGIVLAVFIPTRPPPNLKALMMQADAILTAETQRGQEILRF
                     GPSEPALEALDAIHDRLESPADRMLRQVAPRSSYLVLPLFALANAGVEMSTGVFSGHA
                     PLMIGTAIALAIGKPLGFVIATAAAARSGISVKPQGYSWVQLAGAGCLAGIGFTMSLF
                     IASEAFAVPEDFAAAKIAIFAGSIFSAAAGVAILWNAERISELPVPIRKILRQQRRQS
                     "
     gene            complement(3082..3348)
                     /locus_tag="VFI43_03130"
     CDS             complement(3082..3348)
                     /locus_tag="VFI43_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061152.1"
                     /translation="MSEDLFQFPITGWEIKAEPVEGILSVRFPFLSHEQQKLSDADPG
                     RPYIMQAEQARELRDALSRAIDRLEKQESASPLSTDLSNSLLVY"
     gene            3495..4376
                     /locus_tag="VFI43_03135"
     CDS             3495..4376
                     /locus_tag="VFI43_03135"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7061153.1"
                     /translation="MIVVTAASGKLGRAVAEELGKRVPASDIKLAARTPAKLEFWKNR
                     GFGVVRADYDDPATLNAAFAGAEVVLLISSDGPNDARIRHHKNAIDAAKRTGVKRLVY
                     TSFVNPTPRSKFIWAIAHAETEPYLKASSVPYTILRDNQYASNLDGLIAKAKETGTFA
                     LPGATGKVAYVTHADVAACAAAVVTQRGHEYKTYELTGPEALDGFQIAGVISEASGRE
                     IKTVDLSPEIAAANLRAAGLPEFAVEGIVSMYAAAKAGEYATVTHDVKLLSGRPATSV
                     RSHIKTIVRRELASATG"
     gene            4616..6220
                     /gene="nadB"
                     /locus_tag="VFI43_03140"
     CDS             4616..6220
                     /gene="nadB"
                     /locus_tag="VFI43_03140"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET7061154.1"
                     /translation="MPQFNFDTLIIGSGLAGLTLALNLAETRKVGLVTKRALMDGASS
                     WAQGGIAATLSEEDSPEAHIRDTLMAGAGLCDEEVTRYVVENGPRAIQWLIGQGVPFT
                     RDQGNETGYHLTREGGHSVRRVIHAADATGQAVQLTLIEKARAHPNIRVLEHHVAIDL
                     ITNAKLGHLAGHLSEARNRCRGAYVLDSKAGEVRTIGASSTVLATGGAGKVYLYTTNP
                     DTATGDGIAMGWRAGCRIANMEFVQFHPTCLYHPHAKSFLISEAVRGEGGVLKLPDGS
                     RFMHWHDPRAELAPRDIVARAIDFEMKKRGLDCVYLDISHKPADFLTEHFPNIYKRCL
                     EFGIDITKQPIPVVPAAHYTCGGVVTDFNGKTDIDNLYAIGETAHTGLHGANRLASNS
                     LLECLVFGQAAAKDIMAQPAEPPLELPRWDESRVTNADEEIVISHNWDELRRFMWSYV
                     GIVRTTKRLERAGHRIELLHEEINEYYAHFRVTSDLLELRNLVDTADLIVQSAMLRHE
                     SRGLHYSRDYPRPLLEARDTVLRRSG"
     gene            complement(6303..9641)
                     /locus_tag="VFI43_03145"
     CDS             complement(6303..9641)
                     /locus_tag="VFI43_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061155.1"
                     /translation="MAEQSNDKPKFPLAGLFALLGMISGLLFYEGISLKTSRPVDKEK
                     ATNILVKKSVVQSRLWQDPFEAMEAHQALEQKLTGQPEEKNGEHTLDKLIEVIEQSGI
                     SNLRILPVFVDGSPYVNGAESRLNDRYAVVSALGAAGYLPESGEYIRFFKWRRTERGK
                     QENDQEKKTGTRSATPAEEIETLVPAEFYLPKAELRHQPYGKPVLVLWLKEQDANANP
                     LLFLNDLLAYFRKGTSKASPGISLAYDVVGPHSSATFSAMLKELEPARSGAPALPFNM
                     LAEARFFSPWATAEDTFLLDYSPEPEASVGLDKGGQRKERERKPADELLAEAKIHITR
                     TIGTDAALAEQLLQELRRRQIDLKPCMDKHCNPKVALISEWDTLYGRALPRTFAAVTM
                     NKGSGQTGPDLEAEINKLRRDEWPGWIYRHSYLAGLDGELPVRAGDRSDDKDKDSNQS
                     GARMLPGGKPQATTRNSAPRPEGRGQLDYVVRLAAVLKQEEAKNGEEFKAIGVLGSDV
                     YDKLLILQALRHTFPRAIFFTTDLNARLSYPTEWQWTHNLVIASHFGLELQPALQIPI
                     PPFRDSYQTSLFYSTLWALDHFIAAGSPECPECFRLRRAAPELGTGKPAGPVLKFSSH
                     DKPRLYEVGRQGAFDISMSAHSSLANAASIHPPRHDLGAYTDPTRNLRLALGATATIA
                     ILVLGAMLLSNTAAAAVFKLTSRVGFWIFLILTGLGVYGAAIWVRRSTPDLSQNEPFV
                     LTEGISAWPTAVIELLALLMSLIFLWSSWRKLKGNEKILTHEFGLEGRAIKGTNGKSQ
                     NDFPMDYLPENKLSATGPLSGSLPFGSFRRGVEYLTGLNTWRSPAAGEVDAAQLWSEY
                     ETLGSLKNVFIRCLPQALVALCFIWLMMALFGFPNIPCRGGACFAINKILLAASITTL
                     VFLVCYVLDVTILCRRWVSCIAANRISWPEATLEKISAEQGVARQNLNEWLGIELIAE
                     RTKVIGNFIYFPFITMLVLGIARYRYFDNWDFPSALILVFALNAILLVGNALTLRRSA
                     EAARREAIRRLKSKLTGISGQIPEEIKQRQQIAWAMDTIRNNQRGAFLPFTQHPIFGA
                     AIALPSGGYGLVLLLEYLATGVQN"
     gene            complement(10941..11072)
                     /locus_tag="VFI43_03150"
     CDS             complement(10941..11072)
                     /locus_tag="VFI43_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061156.1"
                     /translation="MIKYGGCVWGHLEAHDFFSLGPAAVIETIAIFRFKAGRGWFSA"
     gene            11358..13841
                     /locus_tag="VFI43_03155"
     CDS             11358..13841
                     /locus_tag="VFI43_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HET7061157.1"
                     /translation="MKSDKTDHATGNQVPHRHHHFHYTTLYHSLACLVLAVSFISPPV
                     ALADPGILPQAAGKRYNIAEGDLGEALERFAAQADISVFFHPEDVKGKTGALNGEFTV
                     QAGLRQLLSGSGLEAAPEADGYRVKKSTGATPDQPAMLPAIKVSASAATGPTDGYMAT
                     KSFSATRTNTPLRDVPQSITVVTQDLIKDQTMQNMGDVVRYVPGVGISQGEGNRDAVI
                     FRGNNSTGDFFVDGLRDDVQYFRDLYNIDRVEVLKGSNGMIFGRGGAGGVINRVTKEA
                     NWAPVNEVAAQYGSFAHKRIAVDVGRPINDAVAVRLNAMYEHSDSYRHGVDLERGGVN
                     PTLTFSPSGQTKIVVGGEYFTDNRTADRGIPSFGTMTNVVGSGRPADTDRSTFFGNAA
                     NSPTHVDVWALNSLIQHTFDNNLTFRNRTRFASYDKFYQNIFGNGVATAEDATGTVAI
                     SAYNNATQRDNIFNQSDFLYDVSTWGIKHEFLTGVEYGRQVTDNLRNTGFFNNTSTSV
                     NVPYFNPLSLTPVTFRPLPFNPANLTGDANNHGVVEVVGIYIQDQITLLPQLKAVLGL
                     RYDNFDLNFLDNRNGQRLHTNDGLLSPRAGLIYKPIETVSIYGNYSLAFVPRAGDQLS
                     SLNLNNAALQPEKFMNLELGAKWDIRPDLALTTALYQLDRSNVIAPVPNDPTRTMLVD
                     GQRVRGAEISLMGRITPQWSVMGGYAYTDAEITRAITGVRAGATVAQVPKHTVSMWNR
                     YDFTPFLGLGLGVVHRSSMYAALDNTVLLPGFTRLDAAMFVRLNKVLRVQANIENIAN
                     IQYIASANNNNNIMPGAPRIFRLTLVANF"
     gene            14421..15566
                     /locus_tag="VFI43_03160"
     CDS             14421..15566
                     /locus_tag="VFI43_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7061158.1"
                     /translation="MRLADFILRDMEAILAAWEAFAAVQLPAARHMDPLALRDHARQI
                     LQAVSRDLSTSQSREAQTAKSLGRAPILIGAPETAAQIHALLRARSGFDINQLTAEYR
                     ALRASVLRLWGDDSPLESTHLDDIIRFNEAIDQALAESVAFFSREVDQGRNLFLGMLG
                     HDLRNPLQTIQMTASYLAALNAGAKVSEAAGYLINSGRHMHALLDDLLDFNRTNLGLG
                     INITPSDIDLSQLCASALDQLRAANPGRKIDLEVNGDTCGNWDSQRLQQLLGNLVRNA
                     IKYGAADAPVCVDLNGDAAGIVLEVRNSGPVIEPLDLDQIFNPLKRGSDNENKRAADG
                     SLGLGLYIAREIAKGHGGSIEARSDETETVFAVRLPRTYTPSQLCRS"
     gene            complement(15688..15882)
                     /locus_tag="VFI43_03165"
     CDS             complement(15688..15882)
                     /locus_tag="VFI43_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HET7061159.1"
                     /translation="MLKRINQTIRYMGQSVRLMVGIPAYDAYVAHMKKSHPDQPVMSY
                     ESFFRERQEARYGGKVSRCC"
     gene            complement(15918..17990)
                     /locus_tag="VFI43_03170"
     CDS             complement(15918..17990)
                     /locus_tag="VFI43_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET7061160.1"
                     /translation="MNSMTTKLGWLLFAILGAFAFSNVALSRGEPVGAIWIVIATVCI
                     YTIAYRFYSRFIASGVLRLDPTRMTPAYKFNDGLDYVPTNKYVLFGHHFAAIAGAGPL
                     VGPILAAQMGYMPGMLWLLAGVVFAGAVQDFIVLFISTRRDGRSLGDLIKSELGQIPG
                     MIALFGTFFIMLVLLAVLALIVVKALAESPWGTFTVAATIPIALFMGVYARYFRPGSI
                     GEVSLIGFVLLMLSIVAGQYVQEIPAMASLFTLTGEQLTWLLIGYGFVASVLPVWLLL
                     APRDYLSTFLKIGTIFGLAIGVIFVSPQLKMPAFTQFVDGSGPVWSGSLFPFLFITIA
                     CGAVSGFHSLISSGTTPKMIRNETDARFIGYGAMLMESFVAIMALVAASTLEPGVYFA
                     MNSPAAIIGSTAESASQAISQWGFYVTPEMITQTAKDVGENTVISRTGGAPTLAVGMA
                     HILSEFAGGRTMMAFWYHFAILFEALFILTAVDAGTRAGRFMLQDLLGTFIPSMKRTD
                     SMIASLIATAICVAGWGYFLYQGVIDPLGGINTLWPLFGISNQMLAGIALILCTGVLF
                     KMKLDRFAWVTIVPAAWVLVTTLAAAWQKIFHSNPRIGFLAHADKYRDAVANGQVLAP
                     AKSLGQMQQVIFNDYVNASLAALFMLVLISILVFGIRTVLHARALGKPSAREAPFELL
                     PAEALSKS"
     gene            complement(18041..18607)
                     /locus_tag="VFI43_03175"
     CDS             complement(18041..18607)
                     /locus_tag="VFI43_03175"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET7061161.1"
                     /translation="MSTSPEFIDFSAPSVDVARSLIGAILLIDGVGGRIVETEAYDRD
                     DPASHTYPGPTRRNEVMFGPPGHAYVYRSYGIHWCLNFVCQPVSHGAGVLIRALEPLA
                     GLDAMRDRRGLDDVRLLCSGPGRVCQSLGVTGAQNGLPLDKPPFQLLEPTGPVTVVAG
                     PRIGVSKAKDVPWRFGLAKSPFLSRPFR"
     gene            18918..19859
                     /locus_tag="VFI43_03180"
     CDS             18918..19859
                     /locus_tag="VFI43_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7061162.1"
                     /translation="MCISELVNRARRIKISAPLLMVAATLLFATMSLCVKVASTDYSV
                     GEIVFYRGMVGAIMMVLLTRAQGETLHTSLPALHFWRSLTGVSSLALWFYALGKLPLS
                     TAVTLNYMSPIWMVVLLIGASAFRNVGNSERVDVRLVCTVLIGFAGVICILQPILERD
                     QLWAGLMGLLSGLLAALAYLHVAALGKAGEPEERVVFYFSLGSVIAGAIATTFMDEWH
                     AHTLQGVGVLLAVGLLATLAQLFLTRAYNIGRVLINGSLQYLGIVWAYLYGVFLFDDL
                     ITGIALLGMGLVILAGIVAARLRESGSGTGDIKTIPE"
     gene            complement(19916..20590)
                     /locus_tag="VFI43_03185"
     CDS             complement(19916..20590)
                     /locus_tag="VFI43_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HET7061163.1"
                     /translation="MKKFAHICLSGFILLFSGTSLAENGAVSITVGSKRGSVDNSALH
                     QVREVVGKAVSTGTVDVFYVYLPRKGSAASTEFGLSGCAESGFNSSPKKFKAFVDELK
                     SIRHEDATFIKLELTQRCDDIEPVEPLDCGGILGALCPRLQFCELLAGQCKTRDAQGS
                     CRAIPDVCNAEYDPVCGCDGKTYGNACEAARAQISLDHRGKCKLPEELVCDEGRPENC
                     ASQSRR"
     gene            complement(20724..21167)
                     /locus_tag="VFI43_03190"
     CDS             complement(20724..21167)
                     /locus_tag="VFI43_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061164.1"
                     /translation="MKFSSISTMRPSASALILAGTMLLGVHGIANAQSGNSGQAKENP
                     ADYSDKKDPIEGRTGEYKGSSNGSSGDKNKAGSSQRGQKDKYGVPQVKEDTEMGSVDQ
                     QKRAERNKDPRSPSPDSHVPDFPTDKQGRPLKDLSKEQGGPIGPN"
     gene            21895..23247
                     /locus_tag="VFI43_03195"
     CDS             21895..23247
                     /locus_tag="VFI43_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7061165.1"
                     /translation="MALLLAPLRISGSAVGPIFFKFGTLLFKFGTLFILTRPGVAYAQ
                     AVFFETKGGSPKIRSDDGNLEIVFGARAHLDVHAFSADKASPGEVPFGSQLPGAFPDD
                     GFNFRRGYTDVTARYGPVSFKFQNDFAAGTFPGSLREVWVGAKLGPGQLTVGQFKAYR
                     GMEELASSNEITMMERPSTSSTGIYSGRQWLMGVGYKGIFRDQIGYGVDIMSLTHTGM
                     PISGSSYGGRMFWVPVADEGNIFHLGVAYSVDTSSAESLAARVADIYGGRRGISKSLG
                     VAGASASPSDKSSQSTFAAEAAYAIGPVTLQGEYVNSTLDDTHQVSGIQKNSRVQAYY
                     VQASWFVTGERVIYRKERGALGKPKPKGKWGALELAFRYDVAENTTQSLTADPCGTGT
                     SKCEVTVITLGANWYVRQGLRFMLNYYITEAAIGNMGPGTPNRTDQPSVISFRTQLSF
                     "
     gene            complement(23359..23925)
                     /gene="efp"
                     /locus_tag="VFI43_03200"
     CDS             complement(23359..23925)
                     /gene="efp"
                     /locus_tag="VFI43_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7061166.1"
                     /translation="MAKVLATEIRVGNLIEWDKRIWRVLKSYHVHVGGRGGAFMQVEM
                     KDIEAGTKTNQRIRTEDKVERAFVEPREMTFLYQDGNNYIFMDKENYEQLSLSQEFLE
                     GQYEYLLPNTDVQVNFHNDRAIGLQLPSSVVLTIIDTEPNLKGATATSSYKPAKMETG
                     LVVMVPPFILQGEKIKINTDSGEYIERV"
     gene            24053..>24317
                     /locus_tag="VFI43_03205"
     CDS             24053..>24317
                     /locus_tag="VFI43_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7061167.1"
                     /translation="MVKTKHPDYYARPVPPFGDEAPKLLIIGLAPGMHGANRTGRPFT
                     GDFAGILLYRTLYKFGFANHEGSASPDDNLRLIQCRITNAVKCL"
BASE COUNT         5467 a         6483 c         6757 g         5610 t
ORIGIN      
        1 attcctctgt tttcgtcgat gcgatcatcg aagcacgaaa ctttgctgtc cgtctcgctc
       61 aacatgcaaa ccgtatccac cccgtcggct gcgaccgctt cccattgcga ggcgctgacg
      121 gctcgtcttc ctgaaggtaa agccgcattt ttcacgctga tgacaattcc cccagatgat
      181 ccgtcaatat aaaaggtgac gacctcacgg gttgcatctc caacggcata aaggcgcaag
      241 ctcgggttaa ccgtttgcgc ccctttactg tcgagtgcag gagcagaggc cagccccgat
      301 atctcgagaa gcgggatcgt gtatcgattc gtgacgacca gttcacgaga ggaattttcc
      361 cgggcggaaa cggatccgga gaacgcaact atagccatgc cggtcaggat gcaaccggtc
      421 gttaggtcga tcgacggaaa taatgatgcg ggcatgcggg gaacctgaat cgtgcgcctt
      481 gtgatcatcc cccgatttta ccagcaacgc gcttcacgag gacttggtta gaagtccttt
      541 aagtccgatg ggcaatgcgt gtgagggacg taagatgcct tgggaaataa aaaaagttcc
      601 ccggtacctg gttccctcgg gcggctcaag cccggtagca gtgattgtgc gccgggcttt
      661 cttttcgctg agaactgttc agtgttccag ccgggcttcc attgtgatat cggccttgaa
      721 aagcttggag acagggcagc cggatttggc cttgtgtgca atttcctgga acttggtgtc
      781 atccgcgccc ggtacctttg cagagacgtt caggtggctt tgggtgattt caaaaccctc
      841 tcccactttc tcgatcgtca cgtcagcgtg agtgtcgatc cgctccggcg tcatccctgc
      901 ttcccccagc ataagcgaga gcgccatcga gaagcaggcg gcatgcgccg cggcaagcaa
      961 ttcttccggg tttgtgcctt cgcctttctc gaatctggat ttgaagccat aaggcgcgtc
     1021 atggagtacg ccggtctcgg tggagattgt gccgctgcca tccttgatgc cacctttcca
     1081 aactgcccat ccctttctgg ttgtcatgat tgcctccttt gttgtcgtaa tatcttccgg
     1141 attggaacag ggagttcact tatccgctcc gcgttccaca gtatggcgac gcccgcggcg
     1201 gccgaaaata tcgagcctgc gaaaatcgca atctttgctg cagcgaagtc ctcgggtacc
     1261 gcaaacgctt cgctggctat gaagagggac atggtaaagc ctattcccgc caggcagcct
     1321 gccccggcca actgtaccca cgagtaacct tgaggtttga ctgagatccc ggaccgggct
     1381 gccgcagccg ttgcaatgac gaatcccagt ggtttgccga tcgccagcgc gatggcagta
     1441 ccgatcatca ggggagcatg accgctgaaa accccggtgc tcatctccac gcccgcattc
     1501 gccagcgcaa acaggggcag aaccaggtag ctcgatcgcg gcgctacctg cctcagcatg
     1561 cgatctgccg gtgattccag ccgatcgtga atcgcgtcga gggcttcaag ggctggctcg
     1621 gaagggccga accgcaggat ttcctgcccg cgctgggttt ccgcggtgag gatggcgtcc
     1681 gcctgcatca tcagcgcctt gaggttgggg ggcggccggg tgggtatgaa aacggcgagt
     1741 acgatacccg aaagggtgga gtgtattccg ctcgcatgca cgcagatcca tagtccgatt
     1801 ccgagcaaga cgtaaggggt tgcgcggtaa accccgccct tattgagcac tgccagcaag
     1861 ccgacaatgc cggctgcagc tccaagatac gcaagatgaa gttcgccgga gtagaacaat
     1921 gcgacgatga tgatggcgcc aatgtcgtcc acgattgccg ctgcggtcag aaacacgcgc
     1981 agttcgatgg gaaccctgtt ccccatcata gcgatcagcg cgactgcaaa cgccgtatcg
     2041 gtcgcggttg gaacacccca gcctcgcagc cattcacctt cggggccggc tggtacccat
     2101 agcaggtaaa gcaacgcggg gaccgccatg ccgccgatcg cggccacgat ggggaacatc
     2161 gctgcctgaa ggctggccag gtgacccacc gtgaattcgc gtttgatttc caggccaacg
     2221 accaaaaaga agacgaccag gaggccgtca ttgatccatt ggcgcaatgg catactaaaa
     2281 gccgcggttc cgaaactgag gccgacgggc tgttgccaga gataattgaa gctggggcca
     2341 agcggcgaat tgctcaggat aaccgccatt cccgtggtga tcagcagcag gacaccggtt
     2401 gatggcgccc actttgcgaa atcgagcgct gctgcgccaa ccacatggcc gagcgagccg
     2461 agcatggcct ccgacagcga attggcgtcc cacggcccgt cgtatcgccg ctcgttgata
     2521 aagaaggtgg gagtaacgat cgcgccgctc gctgccgcgc tttcgatgtc ggcgtctact
     2581 cgggttttcg ccagcagcgc cgctgccggg gatacgtcgc cgagctggaa ttcggcagca
     2641 acttttctta ggtcttcctc ggttagctcg tcggaccggc ctatcagcgc aacgtgcatt
     2701 ctccaaaagc gcgacggatc gtcggatgat tccaccattt cggccgcctg ccgggctatc
     2761 tggttgcctg cgaggggacg gtgacggaat acgtaacgga gacggttccc gaaccggttg
     2821 cgcatctgcg caatccggtc atgcgccagg cgagaggcgg ggtcggcgta acttccatat
     2881 tccaccagcg taatttccgc atcggccgga cccagcacgt gatcgtatgt attatcgacc
     2941 gggcggtcga gcttattggt cggggactcg ggagtcatga acgatgagcg tggttactcc
     3001 gtgccagtac tcgcattgct gcggaacagc ggaatgccga tgtccggatc gcgcgaacca
     3061 ggcttcccct ggttgcaaag cttagtaaac gagcaagctg ttcgacagat ccgtcgaaag
     3121 gggggaagca gactcttgtt tttccagccg gtcaatcgcc ctggacaagg cgtcgcgaag
     3181 ctcgcgagcc tgctccgctt gcatgatata gggcctgccc gggtcggcgt cggacagctt
     3241 ttgctgttcg tgggataaaa aaggaaagcg gacagagagg atgccctcga ccggctcggc
     3301 ttttatttcc catcctgtaa ttggaaattg aaaaagatct tcgctcatag gatcccgatt
     3361 cctgcttttt aaagaaaacc ggagtctagc agagcactcc gacttccttc gttgcctagc
     3421 gcacatactc acgggaggcg cagtgatatg actatacctt gcccgcaagc caaccccacg
     3481 atgaggaggc cgccatgatt gtcgttaccg ctgccagcgg gaaactgggc cgcgcagtcg
     3541 ctgaagaact tggtaagcgt gttcctgctt ccgacataaa actcgcggcg cgaactcccg
     3601 ccaagctcga attctggaag aaccggggct tcggtgttgt ccgagccgat tatgacgacc
     3661 cggccactct caacgccgcg ttcgcaggtg cggaagtcgt tcttttgatc tccagcgacg
     3721 gaccgaacga tgctcgcatc cggcaccaca agaatgccat cgacgccgcg aaacggacgg
     3781 gagtcaagcg tcttgtatac acgagctttg tcaatccaac gccgcgaagc aagttcatct
     3841 gggcaatcgc ccatgctgaa actgaacctt atctcaaggc atccagcgtt ccgtatacta
     3901 ttttgcgtga taaccaatat gcatccaatc tcgatggctt gattgcaaag gcgaaagaaa
     3961 cgggaacatt cgcgctgcca ggcgcgacag gaaaggttgc ttatgtcact catgcggacg
     4021 tggctgcctg tgcggcggct gttgtcacgc aacgggggca tgagtataaa acctacgaac
     4081 tgactggccc cgaggcattg gatggctttc agatcgccgg tgtcatctcg gaagcatcag
     4141 ggcgggaaat aaaaacggtg gatctgtcgc ctgaaattgc cgcagccaat ctaagggcgg
     4201 cgggattgcc ggagtttgct gtcgaaggca ttgtcagcat gtacgctgcg gcgaaggcgg
     4261 gggagtatgc gacggttact cacgatgtga agctcctgtc gggacggcct gccacttcgg
     4321 tacggtcgca catcaagaca attgtcagaa gagagcttgc cagtgcgacg gggtagttct
     4381 taacggtaat cggaaaaccc cttttggaaa accgctttgg aaaaccctgt aagcaacggg
     4441 aatagaaaat taattaatac gtcgtgggaa agattcccgg gtttcacgtc ctgccgtcat
     4501 cctccggtct gctacaaact cctggtttaa acacgaccct cgaccacggc gataataagc
     4561 ggataaaagg tctgttatca ttccaaggtt ataggaatcg cggtaacaga tacgagtgcc
     4621 tcaatttaat ttcgataccc tcatcatcgg gagtggcttg gccgggctca cgcttgcgct
     4681 gaatctcgcg gaaacgcgca aggtggggct ggttacgaag cgcgccctga tggatggggc
     4741 gagcagctgg gcgcaggggg ggatagctgc gacgctgtca gaggaggatt caccagaagc
     4801 gcatatcagg gatacgctaa tggcgggagc gggtctgtgc gatgaagaag tgacccgtta
     4861 tgtggtggaa aatggcccca gagccataca gtggctgatc ggccagggcg ttccctttac
     4921 gagggatcaa gggaatgaaa cgggttatca cctgacacgg gaaggggggc atagcgtccg
     4981 gcgagtgatt cacgcggctg acgcgacggg ccaagcggtt cagctcacct tgatcgagaa
     5041 ggcgcgcgcg catcccaata ttagggtgct agagcatcat gtcgccatcg atcttattac
     5101 caacgcaaag cttggccacc tcgcgggcca tttgagcgag gcgcgcaatc gctgccgcgg
     5161 cgcgtacgtg ctggacagta aagccggtga ggttcgaacc atcggggcga gcagtacagt
     5221 cttggctacc ggtggcgcgg gcaaggttta tctctataca accaatcccg acacggcgac
     5281 gggagacggg atcgccatgg ggtggcgcgc cggctgccgg atcgcgaata tggaattcgt
     5341 gcagttccat cctacttgcc tgtaccatcc gcatgctaaa tcatttctaa tcagcgaggc
     5401 agtacggggc gagggtggtg tgctcaagct tccggacggg tcgcgcttca tgcattggca
     5461 cgatccccgg gcagagctcg cgccgagaga tattgtcgcg cgcgccatcg acttcgaaat
     5521 gaagaaacgc ggccttgatt gcgtctatct cgacatctcg cacaagcccg ccgatttcct
     5581 gaccgagcac tttcccaata tctacaagcg ttgcctggaa ttcggtattg atattacgaa
     5641 gcagccaatc ccggtcgtac ccgctgccca ctatacctgc ggtggcgttg ttactgattt
     5701 caatggcaag accgatatcg acaatttata tgcgatcgga gaaacagctc ataccggcct
     5761 tcatggagca aatcggctcg cgagcaattc tctcctggaa tgcctggtgt tcggtcaggc
     5821 ggcggccaag gacatcatgg cacagcctgc cgagcctccc cttgaattac ctcgctggga
     5881 tgaaagccgc gttaccaatg cagatgaaga aatcgtcatt tcccataatt gggacgaact
     5941 gaggcgcttc atgtggagct atgtcggcat cgtgcggact acgaagcggc tggagcgggc
     6001 cgggcaccgc atcgagctgc tgcacgagga aatcaatgag tattatgctc atttccgggt
     6061 cacaagtgac ctgctagaac tgcgcaactt ggttgatacg gcggatctga ttgtacaaag
     6121 cgccatgctg cgccatgaaa gccgcgggct acactatagc cgggactacc ccaggccact
     6181 gctcgaggcg agggatacag tgctgaggcg gtccgggtaa ttgcatgcat tgcaatcgcg
     6241 gtaaagcgta aatcgctggg agtcgggtaa agagtcctaa tgaaacaaag tgttagcagg
     6301 ccctaattct gaacccccgt cgccaggtat tcgagcagga gcacgagacc gtatcccccc
     6361 gagggcaacg cgatcgcggc gccgaatatg gggtgctgcg tgaatgggag aaacgcgcct
     6421 ctctgattgt ttctgattgt gtccattgcc cacgcgatct gttgcctctg ctttatttct
     6481 tccgggattt ggccggatat ccccgtaagc ttgcttttca gccgcctgat tgcttcccgt
     6541 ctcgccgcct cggctgatcg ccgtaacgtc agcgcgttgc cgacaaggag tatggcgttc
     6601 agggcaaaaa caaggatcag cgcggatgga aagtcccagt tgtcgaaata gcggtagcgc
     6661 gcgattccga gaacaagcat cgtgataaag ggaaagtata taaaattgcc gataaccttg
     6721 gttcgttctg cgatcagttc gatacccagc cattcattca agttttgcct tgcaacgcct
     6781 tgctcagccg atatcttttc cagagtggct tccggccaac tgatcctgtt tgccgcaata
     6841 cagcttaccc agcgccggca aagaatcgtg acgtccagca cataacatac caggaacacc
     6901 aacgtggtaa tgctggctgc caggagaatc ttattgatcg caaagcaggc tcctccgcgg
     6961 catgggatat tgggaaaacc gaacagcgcc atcatcagcc agatgaagca gagtgcgacc
     7021 aaagcttgtg gcaggcaacg gatgaagacg tttttcagac taccgagggt ctcgtattca
     7081 ctccagagtt gcgcggcatc gacctctccg gctgctggcg aacgccaggt attcaaccct
     7141 gtaagatatt ccacccccct gcgaaagctg ccgaacggaa gggagcccga gagcgggcct
     7201 gtggccgaca atttgttttc ggggagataa tccatgggaa agtcattttg agacttgcca
     7261 ttcgtcccct tgatcgctcg cccttcaagc ccgaactcat gggtcaatat tttttcgtta
     7321 cctttcaatt ttctccatga agaccacagg aaaatcaggc tcataagcag ggcaagcagt
     7381 tcgattactg ccgtaggcca tgcgctgatc ccttcggtca gcacaaacgg ttcattttga
     7441 gacagatcgg gtgtggagcg ccttacccat attgcagcgc cgtaaacccc cagacctgtg
     7501 agtatgagga atatccagaa cccaaccctc gaagtaagct tgaaaacggc cgcagccgca
     7561 gtattgctaa gcagcatggc gcccagcacg agaattgcaa tcgttgctgt tgccccaagg
     7621 gccaatctca gatttcgtgt tggatcggtg taagctccga ggtcgtggcg gggcgggtga
     7681 atgcttgcgg cattcgcgag tgatgaatga gcgctcatgc taatgtcgaa ggccccctgc
     7741 cgcccgacct cgtacagcct tggcttgtcg tgactcgaaa atttcagcac cggaccggct
     7801 ggcttcccag tccccaattc tggtgccgcc cgccttaacc ggaagcattc cgggcattct
     7861 ggagaccctg ccgcgatgaa gtgatcgagc gcccacaggg tggaataaaa cagggatgtc
     7921 tggtagctgt ctcgaaacgg tggaatgggt atctgaaggg cgggctggag ctcaagaccg
     7981 aaatgggacg caatgactag attatgtgtc cattgccatt cagtgggata actcaatcgg
     8041 gcattgaggt cggtggtgaa aaagattgca cgtggaaaag tgtgacgcag cgcctgcaaa
     8101 atcagaagtt tgtcataaac atcgctgccc aatacgccta tcgccttgaa ctcttccccg
     8161 ttttttgctt cctcctgttt aaggactgct gcgagtcgca cgacataatc cagttgccca
     8221 cggccttcgg gtcgtggggc cgagttccgg gtcgtggctt gaggcttgcc gcctggcaac
     8281 atccttgctc cgctttgatt gctgtctttg tccttgtcgt cgcttctgtc accggcgcgc
     8341 accggcagtt cgccgtcaag acccgcgaga tacgaatgcc tgtaaatcca gccaggccac
     8401 tcgtccctgc gcagcttgtt gatttcagct tccaggtcag gcccggtttg cccggagcct
     8461 ttattcatcg ttaccgccgc aaaggtgcgc ggaagcgcgc ggccatagag cgtatcccac
     8521 tcggagataa gcgcaacctt cgggttgcag tgcttgtcca tgcagggttt cagatcgatc
     8581 tggcggcgtc tcagctcctg aagaagttgt tctgcaaggg ccgcatcggt gccgatcgtc
     8641 ctcgttatat ggattttcgc ctcggccaat agctcatccg ccggttttct ttcccgttct
     8701 tttcgttgcc cgcccttgtc cagccctacg gatgcttccg gctccggtga atagtcgagc
     8761 aggaaagtgt cctctgcagt tgcccaggga gagaagaaac gggcctcagc caacatgttg
     8821 aaggggaggg cgggcgcgcc ggaccgggcc ggctccaatt ccttcagcat tgcgctaaag
     8881 gtcgctgagc tatgcggtcc aaccacatca taagccagac ttatgccggg gctggccttg
     8941 ctggtgcctt tcctgaaata agcaagaagg tcgttcagga acaagagcgg gttggcgttt
     9001 gcatcctgct ccttcagcca caggaccagc actggttttc catatggctg gtgccggagt
     9061 tctgcttttg ggagatagaa ctcagccggt acaagtgttt ctatttcctc cgccggcgtt
     9121 gcagaccttg ttcctgtctt cttttcctga tcgttttcct gcttccctct ttcagtccgg
     9181 cgccatttga aaaaccggat gtactcgcct gattcgggca aatagcccgc cgcgcccaga
     9241 gcggatacaa cggcgtaacg gtcgttcagc ctggattcgg caccgttgac gtatgggctt
     9301 ccgtccacga acacgggcag gatgcgaagg tttgaaatgc ccgattgttc gataacctcg
     9361 atcaacttgt cgagcgtgtg ctcgccattt ttttcttcgg gctgccccgt cagcttctgt
     9421 tcgagcgcct ggtgggcctc catcgcttcg aatgggtcct gccaaaggcg tgactgcacc
     9481 acgctcttct tcaccaaaat gttggttgcc ttttccttgt cgacaggccg ggatgttttg
     9541 agcgaaattc cctcatagaa cagaagaccg ctgatcattc ccagcagtgc gaacaggcct
     9601 gccagcggaa acttcggttt atcgttggat tgctctgcca taggtcaact ttgtccaggc
     9661 aaactctgcg gcgaagtgcg ggtttcaacg ccgacgcggc cggacgtatt agagtgccat
     9721 ttaatgtcaa gtatagatga gctgatggcg cgaggacgct ttcgctcgca tgcgaccccc
     9781 caaggttcct tatggcaatt cggcaggccg ccgtatatga gaaccagctt cccttccgcg
     9841 ccttcccccg ggctgtactt tagtacaata gacattgcct gtgcacttgg taagattcaa
     9901 tcaggggaag cgaggacgag aagtggaagg cagtgagcag gtgcttcccc ctttgtagtt
     9961 ggaagcaaac gccacggccc ggttgcagga acatctgcac cgggcttttt tatgtcctgt
    10021 agagccggag cgttctctcc tgcgaatgct gttgatacag ttacagttgc atgggatgaa
    10081 ccggaaccca caatacgcgc cgaagtgata aacgagtggg aaaaccggat caaaggggat
    10141 ggcttcagct ccagactttc ctttcactat tctcctgaaa gcgagaagag ttacgtttct
    10201 ttcgtcattc ccgcggaagc gggaatcgag ttttctcgga agtttttgct ggattcccgc
    10261 tttggggaat gtggtgacta gctgatgtgg taacccggga gtgaccggat gcaggggacg
    10321 gccggcgaag tgagtagata gggattaagc ctgccactgg tgaggagcag tatcggattt
    10381 attctcttcg atatttcgat cctggagcgt taatgggcac ggatcctggt gcttatattt
    10441 tggcagcaag cgcaatcgcg taaggcaatg acacgccggt gctggaaatc aagggggaga
    10501 attcgggccc gggtcgcgtc aaaacgagtg gccctggatg tttgggtcaa agctgtgaac
    10561 tggaagggcg gttcgccact tgggcctggg atgtcgtatt ccaacctgga cagatacagg
    10621 atggtgataa cgaagtggag ccgcgggtaa cgggggcagc atcgtgattt ttcgaggggc
    10681 tgctgctact gatttgccgc atgcaaaacc cgcgctctcc ctcgtcctac cgagggactt
    10741 caaataaggc tttgttttcg tcaggaaatt attgcgcgac gaccaagctg cttgaaaatg
    10801 tcttggtcgc cctctctccc ggcctctctc ccaaaaatgg gagagaggag attaatgggg
    10861 tttctgggtc tcaagccggc gagccataca ccctgaaatc tctcccgcga gggagaggaa
    10921 aattaaggag cctcaaacca tcaggcggaa aaccatcccc gcccagcctt gaaccggaat
    10981 attgcgatgg tttcaatcac cgcggccggc ccgagcgaaa agaagtcgtg cgcctccaga
    11041 tgcccccaga cgcatccccc atacttgatc atttttcctt taagccctga taggtctcct
    11101 gcagtaggcg agaaaccttg ttgttgcctt tgagtctcct tagcaatata ataagaatca
    11161 ttctcattcg taatacgagg gggtaaatta gcgtttcact gtttctgttt caattgatct
    11221 cttcaacctt ccatacgcaa atttctgaac aaataatttc atttatacca ccgcgttttt
    11281 tcgcgacgtg gtctttcatc agccagccag ccttcagcaa gccagcggat tactctgtct
    11341 cgttaaagga tatcggcatg aaaagcgata aaacggatca cgcaaccggt aatcaagttc
    11401 cgcaccggca tcatcatttt cactacacta cgctctacca ttcactggcc tgcctggtat
    11461 tagctgttag ctttatttcc cctccggtcg ctttggccga cccaggcatc cttcctcagg
    11521 cggcaggaaa gagatacaac atagcggaag gtgacctcgg agaagcgctt gagcgttttg
    11581 ctgcgcaagc tgacatcagc gtctttttcc accctgagga tgttaaagga aagacgggcg
    11641 cattaaatgg ggaattcact gttcaggcgg ggttaaggca attattgtct ggaagcggcc
    11701 tggaagcagc gccggaggca gacggctaca gggtaaaaaa atcaactggg gcaacgcctg
    11761 atcaacccgc gatgttgcct gcgataaaag tgtcggcgag cgctgcgacc ggcccgactg
    11821 acggatacat ggcgacgaag agcttcagcg cgacgcgaac gaatacgcct ctacgcgatg
    11881 ttccccaatc catcacggtt gtgacgcagg atctgatcaa ggatcaaacc atgcagaaca
    11941 tgggcgacgt ggtgcgctac gttcccgggg tcggcatatc acaaggggag ggcaaccgcg
    12001 acgcggtaat tttccggggc aataattcaa ccggagattt ttttgtcgat gggttgcgcg
    12061 acgacgtgca gtactttcgc gatctctaca atatagaccg ggtcgaagta ttgaagggtt
    12121 cgaacgggat gattttcggg cgtggcggag cgggtggggt aattaacagg gtgaccaagg
    12181 aagcgaactg ggcacccgtc aacgaagttg cggcgcaata tggctccttc gctcacaaac
    12241 gcatagcagt cgatgtgggc cggcccataa acgatgccgt tgctgttcgt ctcaatgcca
    12301 tgtacgagca ttctgacagt taccgccacg gcgttgacct cgagcgcggc ggcgtcaacc
    12361 cgacgcttac gttcagccca agcgggcaga cgaaaatcgt ggttgggggc gaatatttta
    12421 ccgacaatcg tacggcagac cgcggcatac cctcttttgg aaccatgact aacgttgtcg
    12481 ggtcaggtcg tcccgccgat accgaccgct cgacgttttt tggcaatgcg gcaaacagtc
    12541 caacccatgt tgacgtctgg gccttgaact cgcttatcca gcacacgttc gataacaatc
    12601 ttacctttcg caatcggacg cgctttgcca gttacgataa gttttatcag aatatatttg
    12661 gtaatggggt ggcaacagcc gaggatgcga ctggaaccgt agcgatttca gcttataaca
    12721 atgcaacgca acgcgacaat atcttcaatc agtcagattt tctctatgac gtcagtacgt
    12781 gggggataaa gcatgaattc ctgaccggcg tggagtatgg ccgccaggtt acggataatc
    12841 ttcgtaatac cgggtttttc aacaatacca gcacgagcgt aaatgttccc tatttcaacc
    12901 ccttgagcct gacacctgtc actttcagac cactcccttt caatcccgca aaccttaccg
    12961 gggacgccaa caaccacggc gtcgttgagg tggtgggcat ttatatacag gaccagatta
    13021 cgcttctccc gcagcttaaa gcggttcttg gcctgagata cgacaacttt gatctgaatt
    13081 ttctcgacaa tcgtaatggc cagcggctcc ataccaatga tggcttgctc tcgccacggg
    13141 ccggcttgat ttataagccg atcgagacgg tttccattta tggaaactac agcttggcct
    13201 ttgtaccacg tgctggagac cagctctcat cgctaaacct gaacaacgct gcactccaac
    13261 cagaaaagtt catgaacctg gagttgggag ccaaatggga tatccggcca gatttggccc
    13321 tgaccacggc cttgtaccaa ctggatcgca gcaatgttat tgcaccggtt cccaacgatc
    13381 ccacgcggac aatgctggtc gacggacagc gcgtcagagg tgcagaaatc agcttgatgg
    13441 ggcgcattac gcctcaatgg agtgtaatgg gaggttatgc ctataccgac gcggaaatta
    13501 cacgcgcgat aacgggggtg cgggcgggtg caacggtggc gcaagtcccg aagcatacgg
    13561 tttcgatgtg gaatcgctat gatttcacgc cattcctcgg attgggtctc ggtgtggttc
    13621 accgtagcag catgtacgca gccctcgata ataccgtgct ccttccgggc ttcacgcggt
    13681 tggatgcagc aatgttcgtg aggctcaaca aggtcttgcg ggtgcaggcg aatatcgaaa
    13741 atatagccaa catccagtac attgcctctg cgaacaataa taacaacatc atgcccgggg
    13801 cgccgcgcat tttccggttg acattagtag ccaactttta aacccttcgc tttaaacgcc
    13861 tcactcaggc tatcggtatg gacttagaag cactgtcatc caaaagcgga tatagcttgt
    13921 tttcgatagc tggtgaaata taagatgttt ccggtgaggg ggcggcggaa tggatcagga
    13981 taagttatag attttctaat tagtcgatgg ggtagcggta tactggtaag gagaagtgac
    14041 tgtagagtta aatctccatt tttttatctt taaaaaagat ccaatatgtc tttccgcatc
    14101 gtaagattta cgttctcact gtcctgcagg aataagttct ctattagtcc tcggtattga
    14161 ttcctactcg gtattgttgt tagaacctta cgtcttttgt taatgcttgc ccctcctgtt
    14221 gacgttttac tgcccctacc ctcctcatca ttggtgagta cccttaacta ccccgccaat
    14281 accttgcaag ttcggcgaat tcggctattg cagccaagta cgcccttaac agttaacagt
    14341 taagattgtt ctcacagaat tcacggttgt acattcaagc ttcccacata aagtttttct
    14401 aatcatcaaa agtcactgat atgcgattag ccgattttat tttgcgtgac atggaagcta
    14461 ttctcgcggc gtgggaggca tttgccgcgg ttcaactgcc cgccgccagg cacatggacc
    14521 cgcttgcttt acgcgaccac gcgcggcaaa ttcttcaagc cgtgtcaagg gatctttcca
    14581 cttcacagag cagggaagcg caaactgcga aatcattggg ccgcgcgcca atactaattg
    14641 gcgcgccgga gactgctgcg caaatacatg ccctcctgcg cgcacgaagc ggtttcgaca
    14701 tcaaccaact gaccgcggaa taccgcgccc ttcgcgccag cgttcttcgt ctatggggcg
    14761 atgattctcc gctggaaagc acccatttgg acgacatcat tcgcttcaat gaggcaattg
    14821 accaagcgct tgccgaatcc gtggcctttt tcagcaggga agtcgaccag ggccgtaacc
    14881 tatttcttgg gatgcttgga catgacctgc gcaatcccct gcagacgatc caaatgacag
    14941 catcctatct ggccgcactc aatgctggcg ccaaagtatc ggaagctgcg ggctatttga
    15001 tcaacagcgg ccgtcacatg catgcgctac tcgacgatct gctggatttc aaccgtacca
    15061 acctcggttt aggtattaat atcactccta gcgatattga cctcagccag ctgtgcgcta
    15121 gcgcattgga ccagttgcgt gcagcaaatc ccggtcggaa gatcgatctg gaggtgaacg
    15181 gcgatacgtg cggcaattgg gatagtcaac gtctgcagca gttactcggc aacctggtgc
    15241 gtaatgcgat caagtatgga gcggcagatg cgccggtgtg tgtggattta aatggggatg
    15301 cagcggggat tgtcctcgaa gtcaggaata gcgggcctgt catcgagcca ttggacctcg
    15361 accaaatatt taatcctttg aagcggggct ccgataacga gaacaaacgc gccgcggatg
    15421 ggagcctcgg gcttggactg tatatcgcgc gagaaatcgc caaaggtcat ggcgggtcta
    15481 tagaagctcg gtcagacgag accgaaacgg tattcgcggt gagactgccg cgaacgtaca
    15541 ctccaagcca gctttgccgt tcttgactat agccgcaaga cacacgcaca ccccctgaag
    15601 tatttccaga ccggacagaa gcagtggttt gccatggcca gccctgcttt tcccgtattg
    15661 aacccccatt atccgaagag cgaagactta gcagcagcgg ctgacctttc cgccatatct
    15721 tgcttcctgg cgttccctga aaaagctttc ataggacatg acgggttggt ccggatggga
    15781 ttttttcatg tgagcgacgt aggcgtcata ggcaggtatt cccaccatga ggcgcacgct
    15841 ctgacccatg taccggattg tctggttgat tcttttcaac atttttgact ccatgtatcg
    15901 aggtctggaa cgattactca agatttggat aaagcttcgg caggcagcag ctcgaatggg
    15961 gcttccctcg cgctgggctt accaagggcg cgcgcatgta aaaccgttct gatgccaaac
    16021 accagtatgc tgatcaggac cagcatgaac agtgcggcaa gcgaggcatt cacgtaatcg
    16081 ttgaaaatga cctgctgcat ctgccccagc gatttggcag gagcaagcac ttggccgttt
    16141 gcaaccgcat ccctatattt gtcggcgtgt gcaagaaagc cgatgcgcgg attggaatgg
    16201 aatatcttct gccacgctgc ggccaacgtg gtcaccaaca cccatgcagc aggcacgatg
    16261 gtgacccagg cgaagcgatc gagcttcatc ttgaacagca cacccgtgca aagaatcagg
    16321 gcgatgccgg ccagcatctg gttggaaatg ccgaataatg gccacaatgt attgataccg
    16381 cccaagggat cgatcacacc ttggtagagg aaatagcccc agcctgccac acagatcgcc
    16441 gttgcaatca ggctggcgat catcgagtcc gtgcgtttca tcgacggaat aaaggtgccc
    16501 agcaaatcct gcagcatgaa tcgcccggcg cgtgttccgg cgtccacggc cgtcaggatg
    16561 aaaagcgcct cgaacaggat ggcaaagtga taccagaaag ccatcatggt tctgcccccc
    16621 gcaaactccg acagaatgtg cgccatgcct accgcgaggg tcggcgcgcc gccggttcgg
    16681 gagatcactg tattctcccc cacatccttc gcagtctgcg tgatcatctc tggcgtgaca
    16741 taaaagcccc attgtgaaat ggcctgagag gctgattcag cagtgctgcc gatgatggca
    16801 gcagggctgt tcatggcgaa gtacaccccg ggctccaggg ttgacgcggc cacgagggcc
    16861 ataatcgcca caaacgactc catgagcatc gctccataac cgatgaagcg cgcgtcggtc
    16921 tcgttgcgga tcatcttggg ggtagtgcct gaggatatga gagaatgaaa gccggagact
    16981 gctccacagg cgatggtgat gaacaggaag ggaaacaggc tgcccgacca caccggcccc
    17041 gagccatcga caaactgcgt gaacgccggc attttgagct gtggcgagac gaatatgaca
    17101 ccgatggcca gcccgaagat ggtgccgatc ttgaggaaag tggacagata gtcgcgcggc
    17161 gcgagcagca gccagacagg caggacggaa gccacgaagc cgtagccgat cagcagccat
    17221 gtgagttgct cgccggtaag cgtgaacagc gaggccatcg cgggaatctc ctgcacatat
    17281 tgcccggcga cgatcgacag catcaacagg acaaagccga tgagagagac ttctccgatt
    17341 gagccgggtc gaaaataccg cgcatacacc cccatgaaga gggcgatcgg tatggtggcg
    17401 gctacggtga acgttcccca cggcgattcg gccagggcct tgaccacgat gagcgccagc
    17461 actgcaagca ggaccagcat gatgaagaag gtgccgaaca aggcaatcat tcccgggatc
    17521 tggccgagtt ccgattttat gaggtctccc agggagcggc cgtcccgccg cgtggaaatg
    17581 aacagcacga taaaatcctg taccgcgccc gcaaacacga cacctgcaag tagccacagc
    17641 attcccggca tataacccat ctgcgctgcc agtatcgggc ctaccagggg gcctgccccg
    17701 gcaatcgcgg cgaagtgatg tccgaacagc acgtatttat tggtgggcac ataatccagc
    17761 ccatcattga acttgtaagc cggcgtcatg cgggtgggat ccagtctcaa taccccgctg
    17821 gcaatgaacc ggctgtaaaa ccggtaggcg atggtgtaga tgcagaccgt ggcgatcacg
    17881 atccatatcg cccccacggg ttcgccgcgg ctcagcgcga cgttggaaaa tgcaaaggcg
    17941 cccaggatcg cgaataacag ccatccaagt tttgttgtca tactgttcat ttgttctccg
    18001 atttatgaaa agcgacacca ggccatgaac tgtccagtct tcagcgaaag ggacggctga
    18061 ggaaaggaga ttttgccaac ccaaaacgcc aagggacgtc ctttgccttt gatacgccta
    18121 tgcggggacc agccacaact gtgaccggcc cagtgggttc cagaagctgg aaaggcggtt
    18181 tgtcgagggg cagtccattc tgcgcaccgg taaccccgag cgattggcat actcgaccag
    18241 gacctgagca aagaaggcgt acgtcatcca gaccgcgccg gtcacgcatg gcatcgagcc
    18301 cggcgagcgg ttccagcgcc cggataagaa ctccagctcc atgggatact ggctggcaca
    18361 cgaagttgag gcaccagtga atcccgtaag aacggtaaac ataggcatgg ccggggggac
    18421 cgaacattac ttcgtttcgt ctggtaggcc cagggtaagt gtgagaagcg gggtcatctc
    18481 gatcgtaagc ctcggtttcc acaatacgcc cgcctacgcc gtcgataagc aggattgcac
    18541 cgatcaggga tctggccaca tcaactgagg gagctgaaaa gtcgataaat tccggggacg
    18601 tggacatgca gcaattttat acagaagacc tcttttcgaa acgctcttcc cgataacaaa
    18661 aatgattttt tagatagcaa tccgatattg caagacgaat tgtcctttgc tcgaagtttt
    18721 aataatttcc ccttgaagca tgggtgttga atatgggcag gttctggtag tcaaatgtga
    18781 gtttgacgcg atgtccttgg gctggcgacc cttatcaaga agaacagtct ttaaaagtct
    18841 agtcattatt attggctatc ctgcttcctg ggggcgacgg tactcatcat agaagaataa
    18901 gcccctccat agaaataatg tgtataagcg agcttgttaa ccgcgcgaga aggataaaaa
    18961 tatctgctcc gctactgatg gttgctgcca cgctgctttt tgcgaccatg agcctgtgtg
    19021 tgaaggttgc ttccaccgac tacagcgttg gagaaatcgt attctaccga ggcatggtgg
    19081 gagcaatcat gatggtactg ctcactcggg cgcagggtga gacacttcat actagtctgc
    19141 cggctctcca tttctggaga agccttactg gggtgagctc gttggccctg tggttttacg
    19201 ccctcggaaa attgccgctt tccacggctg tgacattgaa ctacatgtct cctatctgga
    19261 tggtcgtcct cctcatcggg gcttcggcat ttcgtaacgt gggtaactcg gaaagagtgg
    19321 atgtccggtt ggtatgcacc gtgctcatag gttttgctgg cgtaatttgc attctccagc
    19381 caatcctgga gcgtgatcag ttatgggctg gtttgatggg cttactgtcg ggtctgcttg
    19441 cggccttggc gtatctgcat gtggctgcgc ttggcaaggc cggtgagcct gaggagcggg
    19501 tggtatttta tttctcgctg ggcagcgtca tcgcgggtgc tatagcgacg acgttcatgg
    19561 atgaatggca cgcacatacg ttgcaagggg tcggagtact gcttgccgtg gggttgctgg
    19621 caacactggc gcagcttttt ctaacgcgcg cctacaacat cggccgggtg ctcatcaacg
    19681 ggagccttca atatctcggt atcgtctggg cttatttata cggagttttt ttgttcgatg
    19741 acctgattac cggtatcgct ttgctaggca tggggctcgt tatactggca ggtatcgtgg
    19801 cagcgagact gcgcgaatcc ggtagcggga cgggcgatat caagacaatt cccgaataat
    19861 gagccctgca ttagagccct gcgctattcg ggttgtgtta ctcgctttta cgatttcatc
    19921 gcctgctttg acttgcgcaa ttttccggcc tgccttcatc gcataccagt tcttccggca
    19981 gcttgcattt tccccgatgg tcgagggaaa tctgggcgcg cgctgcttcg caggcatttc
    20041 catacgtttt gccatcacag ccacacacgg gatcgtattc cgcgttgcat acgtcgggta
    20101 ttgccctgca tgatccttgc gcatcccgag tcttgcactg acccgcgagg agttcgcaaa
    20161 actgcaggcg agggcaaaga gcgccgagaa taccgccgca gtccagaggt tctacgggtt
    20221 caatatcgtc acagcgctgg gtcaattcca gcttgatgaa ggtcgcgtct tcatgacgta
    20281 tagatttcag ttcgtcaacg aatgccttga acttctttgg gctcgagttg aagcctgact
    20341 cggcacaacc ggataaaccg aattcagtcg aagcggcgct cccctttctg gggagataaa
    20401 cgtaaaaaac atcgaccgtc ccagtggaaa cggcttttcc taccacttcc ctgacctgat
    20461 gcaacgccga gttatcgacg ctcccccgct tgctgcccac cgtaatactg accgcgccat
    20521 tttctgcaag ggaagttccg ctaaataaga ggatgaatcc gctcagacaa atgtgggcga
    20581 attttttcat tctttatgcc gggcattgcc ggttcctgca tcggctgatt tgtttacgaa
    20641 cccctgaact aacccctttt ttaccctggg acatgctgct cagggtactg atggagttga
    20701 gtcagaggct ccttttttgc cactcagttc ggtccgatcg gaccaccttg ttctttgctc
    20761 agatccttta ggggccttcc ttgcttgtct gttggaaaat ccggtacatg agagtcaggg
    20821 ctgggagagc gtggatcctt gtttcgttcc gctcttttct gttgatctac ggaccccatt
    20881 tccgtatctt cttttacctg cgggacgcca tatttatctt tctggccgcg ttggctagat
    20941 cccgccttgt tcttgtcacc ggagcttcca ttggagcttc ctttatactc tccggtgcgg
    21001 ccttcgatgg ggtctttctt gtcactataa tcagcaggat tttccttcgc ctgccccgag
    21061 ttgccgcttt gggcatttgc aattccgtgg actcctaaga gcatggtacc tgcgagaatc
    21121 aacgctgacg cggatggtcg catcgtcgaa atggatgaga atttcatggc tgttcctttt
    21181 tcctggccac agcgatattg ctgtgtaacg agattaaccg ctttgactcc aactcttcag
    21241 tgcggtagca cactaaaatt atcgcaacgg tttttgagaa tgtagctggg gcgcggggat
    21301 ggacaaaagg gaaagcgccc aaggaaagtt tacctatctc cgctgagcaa taaagggaag
    21361 caaaaaaaaa gtatccacgc gatcaaggtg acggatagag agcgtatttt tcggtaagag
    21421 ctgacttgag ggtgagtgga caggattaat atctgccttt gattctaggt cccgttccgt
    21481 tatagccgca atgttcatat aagtcccggc gatcggattc agagccttcg cacgggtcac
    21541 cgtactggct gctgctggag tgatagctct catactgccc tctccgggtg gtttcacttg
    21601 ggccataatc cgcgtttctg ccggaaatgc cttctcgcgc agcggaaccc ccagaaggcg
    21661 cgggcgactc aatctgatct gctgcgtatc cttgagcctg agcggtactg gtcaggacga
    21721 gtgcggtagc caacaccgct gatttaatca ataatgtaga ggttttcata gccgtttcct
    21781 ttttatttac cagcgggatt gctgtgcgga taatagacaa ttgtttctta tggttcagtg
    21841 cgttaccaca cgaaaaccaa cgctgtatgt aacgttaggc gaaggtccct tcgcttggca
    21901 ctattgttgg cgccattgcg aatttccgga tccgctgtcg ggccaatttt tttcaaattt
    21961 ggcacgctgc ttttcaaatt tggcacgctg tttattctga ctcgtccagg cgttgcttac
    22021 gcccaggcgg ttttcttcga aacgaagggc ggcagcccca agatcagaag cgacgacggc
    22081 aatcttgaaa ttgtcttcgg cgcgcgagcg catctcgatg tgcatgcgtt cagcgcggac
    22141 aaagcgagcc caggtgaagt gccgtttggc agtcagctac ccggcgcctt tcccgatgac
    22201 gggttcaatt ttcggcgtgg atatacggac gtgaccgcga gatatggtcc ggtgagtttt
    22261 aaattccaga atgattttgc cgcagggacc tttcccggca gcctgcgcga ggtctgggtt
    22321 ggggcaaagc tggggccagg ccagcttacg gtgggccagt tcaaggctta tcgcggaatg
    22381 gaggaattgg cgagttccaa cgaaattacc atgatggagc gtccgtcaac ttcatcgacc
    22441 ggcatttatt caggtcgcca gtggctgatg ggggttggtt ataaaggaat tttcagggat
    22501 cagattggtt atggggtcga tatcatgagc ctgacgcata ctggaatgcc aatctccggt
    22561 tcgagctatg gaggccggat gttctgggtg cctgtcgcgg atgaaggaaa catctttcac
    22621 ctaggcgtcg cgtatagtgt ggacacctcg agcgcggaat ctcttgcagc gagagttgcc
    22681 gatatttatg gagggcgccg aggcattagc aaatctctcg gcgttgcagg cgcgagcgca
    22741 agcccgtccg acaaaagtag ccagtccact ttcgcggctg aggctgctta tgcgataggg
    22801 cctgtcactt tgcaggggga atacgtaaat tcgacgctgg atgatactca tcaggtctcc
    22861 ggtatccaaa agaattccag ggtacaggcc tattatgtgc aagcgagttg gtttgtaacc
    22921 ggggaaagag taatttaccg gaaggagcgg ggagcgttgg gaaaaccgaa gccaaaaggt
    22981 aaatggggag cccttgaact tgccttcaga tatgacgtcg ccgagaatac aacgcaaagc
    23041 ctgactgcgg acccctgcgg taccggcacg tcgaaatgcg aagtgacagt tattactttg
    23101 ggtgccaact ggtatgtgag gcaaggcctg cgcttcatgc tcaattatta tattactgaa
    23161 gctgcgatcg ggaacatggg tccgggtacg ccgaaccgga ccgatcaacc ctccgtgatt
    23221 tcttttcgca cgcagctcag cttctagcaa ggatggaaag ctggaggcta aaataccaga
    23281 gaagcggaat gaggaagcgg gaatgcagga attataaaga ggagtgatga aagagcgatt
    23341 tgcgtgccag ctggagtact acacccgctc tatgtattcg ccgctgtcgg tgttgatctt
    23401 gatcttttcc ccttgcagga taaatggtgg aaccatgaca acaagtcccg tttccatctt
    23461 cgcaggcttg taagagctgg tggcagtcgc tcccttgagg ttaggttcgg tatcgatgat
    23521 cgttaacacc acgcttgagg gaagctgcaa gccgatagca cgatcattat gaaaattgac
    23581 ctgcacatcc gtattgggga gcaggtattc gtactggcct tcaagaaact cctgagacag
    23641 gcttaactgc tcataatttt ccttgtccat gaaaatgtaa ttgttgccgt cctggtagag
    23701 gaaggtcatc tcgcgcggct ctacaaaagc gcgctccacc ttgtcttccg ttcgaatgcg
    23761 ctgattcgtc ttggtcccgg cctcgatatc cttcatttca acctgcatga acgcgccgcc
    23821 tcggcctccc acgtggacat gataagattt cagcacccgc cagatgcgct tatcccactc
    23881 gatcaggttg cccacgcgaa tttctgttgc caaaacttta gccattaaat ttccagtgca
    23941 tcataaaacc agcattttac tacaaaacat accggttaac cccgagacca gagagggatc
    24001 agcattctcc accgattgcc gccggtgcga ccgtcttgcg ggatttctgg acatggtgaa
    24061 aaccaagcat ccggattatt acgcgcgtcc agtcccccct tttggcgatg aggccccgaa
    24121 gcttcttatc attggcctgg cccccggcat gcatggcgca aaccggacgg gaaggccgtt
    24181 cacgggagat tttgcaggca tattgctgta ccgtacgttg tacaaattcg gtttcgccaa
    24241 tcacgaaggt tctgcttctc cggatgacaa tttgcggctc atccagtgcc gtattaccaa
    24301 tgcggtgaaa tgcctgc
//
LOCUS       DASSHJ010000077         6050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1152542,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000077 DASSHJ010000000
VERSION     DASSHJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152542"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /gene="clpB"
                     /locus_tag="VFI43_03210"
     CDS             <1..2460
                     /gene="clpB"
                     /locus_tag="VFI43_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET7061168.1"
                     /translation="DGGTASLLQRAGTNVPPLRDALKKSIERLPKVEGTGGEITVSRE
                     LLNLLNLADKEAQKRGDQFIASEMFLLAAIQDKGETGNLLRQNGANRAALEQAITTVR
                     GGENVGSAEAEGSREALKKYTMDLTERARMGKLDPVIGRDDEIRRTIQVLQRRTKNNP
                     VLIGEPGVGKTAIVEGLAQRIVNNEVPETLKDKRVYSLDMAAMLAGAKYRGEFEERLK
                     AVLKEIAQSEGKIIVFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDE
                     YRKYIEKDAALERRFQKVFIEEPTVEATIAILRGLQERYEVHHGVDITDPAIVAAAEL
                     SHRYITDRFLPDKAIDLIDEAAARIRMEIDSKPEAMDKLDRRVIQLKIEREAMKKEKD
                     EASQRRLGLLEQEIEKLEREYADLEEIWKAEKSQVQGSQHIKEEMEGVKLEMEAARRR
                     GDWQKVSELQYGRLPQLEAQLSVQDRHGEAPAEHKHKLLRTQVGAEEIAEVVSRATGI
                     PVSKMMQGEREKLLHMEEKLHERVIGQDEAVRLVSDAIRRSRAGLSDPNRPYGSFLFL
                     GPTGVGKTELCKALAGFLFDSEDHLIRLDMSEFMERHAVARLIGAPPGYVGYEEGGYL
                     TEAVRRKPYSVILLDEVEKAHVDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNL
                     GSQMIQQMAGDDYQVIKLAVMGEVKTYFRPEFINRIDEVVVFHALDEKNIKAIAQIQL
                     KYLEARLAKIQIALKVTEAALAELAQAGFDAVFGARPLKRAIQSQIENPLAKEILEGH
                     FGPKDTINVDAVNGVIVFNKT"
     gene            2565..3128
                     /locus_tag="VFI43_03215"
     CDS             2565..3128
                     /locus_tag="VFI43_03215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HET7061169.1"
                     /translation="MQYMRTEKQKMLAGELYDATDPQLVTERLLARDLLKRLNDSNDG
                     PEERAKILVELLGAPTDADIQTPFFCDYGTNITLGKKVFMNFNCVILDVSPVRIGDLV
                     LFGPAVQVYTATHPMSACARRKGLEAGKPVVIGSDVWVGGGAIICPGVTIGSRSVIGA
                     GSVVTRDVPEDMFAAGNPCRVIRSLEQ"
     gene            3331..3708
                     /locus_tag="VFI43_03220"
     CDS             3331..3708
                     /locus_tag="VFI43_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7061170.1"
                     /translation="MEIKGAVTALAALAQETRLSVFRLLVEAGNEGMSVGSIGEKLCV
                     APATLSFHLKELSHAGLVASRQESRFIYYMADFTQMAALMTFLTQNCCWGMPQECLAV
                     METALGECCSSPQTKCKPERSTS"
     gene            3705..4175
                     /locus_tag="VFI43_03225"
     CDS             3705..4175
                     /locus_tag="VFI43_03225"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HET7061171.1"
                     /translation="MKRFHVHVAVDNLNANIRFYSAIFGAEPSVLKPDYAKWMMEDPR
                     INFAISQRGAKAGLDHLGFQVDTEEELTTLRSRVGVAEIATRNQPDATCCYARSDKYW
                     TSDPQGIAWETYHTLDNAEIYGVDARPETDEAGACCAPSSSAQGRKHKGAAKCC"
     gene            4384..5525
                     /locus_tag="VFI43_03230"
     CDS             join(4384..4636,4636..5525)
                     /locus_tag="VFI43_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET7061172.1"
                     /translation="MKRSKFTEQQIAFALKQAELGTPVEEVCRKMGISDATFYNWKKK
                     YGGLGPSELRRLKQLEEENVKLKRLVADLSLDKTMLQDVLFKKALKPFRKRILIDELC
                     DRYRASLRQACALLRMSSSVYRYKSRARDSLTLRMRIREIAATRVHYGYRRVHVLLQR
                     EGYRDNHKRIYRLYREEGLSLRLKRPKRNKSARLRQPKHEVTAINQIWSMDFVADALF
                     DGRRLRALTVMDNYSRESLAIEVGQSLKGEDVVNTLNRIASERGLPATIKVDNGSEFI
                     SKVMDKWAYERGIELDFSRPGKPTDNAKVESFNGRLRQECLNAHWFLSLEDARRKIDE
                     WRQYYNETRPHSALQWATPAEFARQAKENALSGRQKESEFSTLERC"
     gene            5802..5877
                     /locus_tag="VFI43_03235"
     tRNA            5802..5877
                     /locus_tag="VFI43_03235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5835..5837,aa:Thr,seq:cgt)
BASE COUNT         1493 a         1506 c         1829 g         1222 t
ORIGIN      
        1 gatggaggaa ccgcatcgtt gctgcaacgg gcagggacga atgttccacc cctgcgcgac
       61 gcgttgaaga agtcgatcga acgcttgccc aaggtggaag gtacgggcgg ggaaattaca
      121 gtgtcgaggg agctcctcaa tttactcaat cttgcggata aggaagcgca gaaacggggg
      181 gatcagttca tcgcatcgga gatgttcctc ctggcggcaa tccaggacaa gggcgagacc
      241 ggtaatctgt tgaggcagaa cggggccaac cgcgcggcgc tggagcaggc gataaccacg
      301 gtgcgcgggg gcgagaacgt cggtagtgcg gaagccgagg gcagccggga agcactgaaa
      361 aaatacacca tggatttgac cgagcgtgcg cgcatgggca agctggaccc ggtcattggg
      421 cgtgacgacg agatccggcg taccatccag gtgctgcagc gccgcaccaa gaacaatcct
      481 gttttgatcg gcgagcctgg cgttggcaag accgcgatcg tggaaggatt ggcccagcgt
      541 atcgtcaata acgaggtgcc ggaaacgctg aaggacaaga gggtgtattc cctcgacatg
      601 gcggcgatgc ttgcgggggc gaaataccgt ggagaattcg aggaacgcct gaaggccgtc
      661 ctcaaggaga ttgctcaaag cgagggcaag atcattgtgt ttatcgacga gctacatacc
      721 atggttgggg cgggtaaggc ggaaggcgcc atcgacgccg gcaacatgct caagcccgcg
      781 ctggctcgcg gagaactgca ctgcgtcggt gcaaccacgc tggatgagta ccgcaagtac
      841 atcgaaaagg acgcggcgct cgagcgccgt tttcagaagg ttttcatcga agagcccacg
      901 gtggaggcaa caatagccat cctgcgcggc ttgcaggaaa gatatgaagt gcaccatggc
      961 gtggacataa cggacccggc catcgttgcg gcggcagagc tctcccatcg atatattacc
     1021 gaccgctttc tgcctgacaa ggcgatcgat ctaatcgatg aagcggcggc gcgtatacgc
     1081 atggaaatcg attccaagcc tgaggcaatg gacaagctcg accggcgcgt gatccagctt
     1141 aagatcgagc gtgaagccat gaaaaaggag aaggacgaag cttcacagcg acgcctcggc
     1201 ctgctggaac aggaaatcga aaagctcgag cgcgagtatg ccgaccttga agaaatatgg
     1261 aaagctgaaa aatcacaggt gcagggctcc cagcacatca aggaagaaat ggagggagtc
     1321 aagctcgaga tggaggcggc tcggcgcagg ggcgattggc agaaggtgtc cgaattgcag
     1381 tatggacggc ttccccagct cgaagcgcaa ctgagtgttc aagatcgaca tggggaagcg
     1441 cccgcggaac acaagcacaa gctgctgcga acacaggtgg gagcggaaga aatcgcggaa
     1501 gtggtttcgc gcgcaaccgg cattcccgtg tcgaaaatga tgcaaggcga gcgggaaaag
     1561 cttctgcata tggaagagaa actgcacgag cgtgtgatcg ggcaggatga ggcggtgcgg
     1621 cttgtctcgg acgccattcg gcgctcgcgc gcgggactgt cagaccccaa tcgcccgtat
     1681 ggctcgttcc tgtttctggg accgaccggt gtggggaaga cagaactctg caaggccttg
     1741 gcagggttcc tgttcgattc ggaagaccat ctcatccgcc tcgatatgtc ggagttcatg
     1801 gaaaggcacg cggttgctcg cctgatcggt gcgccgccag gctatgtggg ttacgaggag
     1861 ggcggctacc ttacggaagc ggtgcgcagg aagccgtatt ccgtgatcct gctcgatgag
     1921 gtcgaaaagg cgcatgtcga tgtgttcaac gtgctactgc aggtactgga tgacggacgg
     1981 atgacggatg ggcaggggcg tacggtggat ttcaagaaca ctgtcatcgt tatgacttcc
     2041 aacctggggt cgcagatgat ccagcagatg gcaggagatg attaccaggt catcaagctt
     2101 gcggtgatgg gcgaggtcaa aacctatttc cggcctgaat tcatcaaccg tatcgatgaa
     2161 gtggtcgtgt ttcatgcgct ggatgaaaag aacatcaagg ctatcgcaca gatccagctt
     2221 aaatacctcg aagcgcggct ggcgaaaatc cagatcgctc taaaggtcac cgaggcggcg
     2281 ctggcagagc ttgcccaggc cggtttcgat gcggttttcg gcgcccgccc gctgaaacgc
     2341 gcaatccagt cgcagatcga gaatccgctt gcgaaggaaa tactggaagg tcatttcggc
     2401 cccaaggata cgattaatgt ggatgccgtc aacggcgtga tcgtgttcaa caagacatga
     2461 tgcaggatgc gggctgccag aggtgctggg tatccctttg gcagcaccgc acaagccgga
     2521 gcatcaagag agacaacacc tgatcctgct ggcaaggacg gcggatgcaa tacatgagaa
     2581 ccgagaagca gaagatgcta gcaggcgagt tgtacgacgc aaccgatccc caattggtta
     2641 ccgagcggct gctggcgcga gatttgctca agcgcttgaa cgacagcaat gacggcccgg
     2701 aagagcgtgc caaaatcctg gtggagcttc tcggagcgcc aaccgatgcc gatatccaga
     2761 cccctttttt ctgcgactat gggaccaaca tcacgcttgg taaaaaagtc tttatgaatt
     2821 ttaactgcgt cattctcgat gtctcgccag tgcggatcgg cgatcttgtt ttattcgggc
     2881 ctgcagtaca ggtctacacg gcaacccatc ccatgagtgc gtgcgcccgg cgaaaaggat
     2941 tggaggcagg caaacccgtt gttatcggct ccgacgtctg ggtcggcggt ggcgcgatca
     3001 tttgtcctgg agtgacgatt ggctcacgat cggtaattgg tgccggcagc gttgtgacga
     3061 gggacgtccc ggaagacatg ttcgccgccg gaaatccttg tcgcgtcatc cggtcgctgg
     3121 agcagtagtg tacgcaagga gtgtcatacc cgcgaaagca cgggaaagca ggtatccaga
     3181 cttccggcgt aaagtgggct ttggaagttc aatcgatgga tttccgcttt ccaggctgtg
     3241 aaaaaactcg cctcacccca tccgacgctc ctgcaagggc aacaccccac atgaattgaa
     3301 tttattctat aattccagta ttatagaaat atggaaatta agggagccgt aaccgcactc
     3361 gcggcgctcg cccaggaaac gcgcctgtcg gtttttcgcc tgctcgttga ggcaggtaat
     3421 gaaggcatgt ccgtgggcag catcggtgaa aagctttgtg tagcccctgc taccctgtct
     3481 tttcacctaa aggaactcag ccatgcaggt ctggtggctt cgcgccagga aagccgcttc
     3541 atctactaca tggccgactt cacgcaaatg gcggcgctaa tgacgtttct tacccaaaac
     3601 tgctgttggg gcatgccaca ggagtgcctg gccgtgatgg aaacggcgct tggggaatgc
     3661 tgttcgtccc cgcaaaccaa atgtaaacct gaaaggagca catcatgaaa cgctttcacg
     3721 tgcatgtcgc tgttgataat ctcaacgcaa acatccgctt ttattcggcg atattcggag
     3781 ccgagccttc ggtgctcaaa ccagactatg cgaagtggat gatggaagat ccccgcatca
     3841 atttcgctat ctcccagcgg ggggcaaagg caggactgga ccatctcggg ttccaggtgg
     3901 atacggagga agaactcaca acattacgca gccgagtagg tgttgctgaa atcgctacgc
     3961 gtaaccagcc ggacgccaca tgctgctatg cgcgctccga caaatattgg accagcgatc
     4021 ctcagggaat cgcttgggag acctatcaca cgctggacaa cgccgagata tacggtgtgg
     4081 atgcgagacc agaaacagat gaagcaggcg cgtgctgtgc gccatcctca agcgctcaag
     4141 gcaggaagca caaaggggca gcgaagtgct gctagcgccg aaagaacgac agcctgagcc
     4201 acgacagtgt catgagcgcc attgttcttg agtctgtcct agtttgctcg cattgtggct
     4261 tctcaaaaca ggagaccatg cgtacggatg cctgtcagtt ctgaccctac cccaataaac
     4321 agcaccgttt aaaagtagaa tttccggcat gattttcacc cttcaaagga aaggagtcat
     4381 gacgtgaaga gaagcaagtt taccgagcag cagattgcgt ttgccttgaa gcaggccgag
     4441 cttggcacgc cggttgaaga agtctgcagg aagatgggca tcagcgatgc caccttttac
     4501 aactggaaga agaagtatgg gggactgggg ccatcggagc ttcgccggtt gaagcagcta
     4561 gaggaagaga acgtcaagtt gaagcggctg gtagcggact tgagcctgga caagacgatg
     4621 ctgcaggacg tgctgtcaaa aaagctctga agcctttccg caagcgtatt ctgatcgatg
     4681 agttgtgtga ccgttatcgc gcgagcttga ggcaggcctg tgcattgctg aggatgtcga
     4741 gttcggtata caggtataag tcacgagcgc gtgattcatt gacgttacgg atgcgtatca
     4801 gggaaattgc ggcaacaaga gtgcattacg gctatcggcg cgtacatgtc ctgttgcagc
     4861 gggaaggcta tagagacaat cacaagcgga tctaccggct ctatcgtgag gaagggctgt
     4921 cgttacgatt gaagcgcccc aagcgcaaca agtctgcccg cttgcgccag cctaaacatg
     4981 aagtgacggc gatcaatcag atctggagca tggattttgt cgccgatgcg ctgttcgatg
     5041 gcaggcgact gcgcgcactg acggtaatgg acaactatag ccgagagagc ctggcaatcg
     5101 aggtgggcca gagcctgaaa ggagaagatg tcgtgaacac cctcaatcgc attgcatcag
     5161 aacgcggctt gcctgccact atcaaagtgg ataatggcag cgagttcatt tccaaggtca
     5221 tggataaatg ggcctatgag cgcggcattg aactcgactt cagccgcccg ggtaaaccca
     5281 ccgacaatgc caaggtggaa agcttcaacg gccgcttacg gcaggagtgc ctgaatgcac
     5341 actggtttct gtccctggaa gatgccaggc gcaagattga tgagtggcgg cagtactata
     5401 atgagacgcg tccccactct gcattgcagt gggcaacccc cgctgagttc gctcgccagg
     5461 caaaggaaaa cgccttgtcg ggcaggcaaa aagagtcgga attttctact ttagagcgtt
     5521 gctgattttg ggggcgggtc attaagaatc ggactctact acgtcatgaa gctaatgaaa
     5581 ggggcaggtc agcgggaagg gcttgtttcg gagaaaatag tattaaaacc ctgtccgagc
     5641 caatggtctt tccgcgcata cggtggcgcc agctgaaaag acatatcatg acaaacccgg
     5701 ttgggatttt agcggcgact ggcgagattt aggcttcttc acgccggttt gccgggcttt
     5761 cgtcaaagct ttataattga aggctcggtc gtttttgcaa ggctgttgta gctcagttgg
     5821 tagagcaact gattcgtaat cagtaggtcg gaggttcgac tcctctcaac agcaccaata
     5881 aattaacgag ttacatgatt tttctcgcca ctcctggact agggtggaaa atgtaaccga
     5941 tttgtaacca agatagcgca ggttgcgtta tttccccctt cgacaatacc tgagtagcgt
     6001 cccagatatt taaatcgatt gcaggacttc tgatgaagat gaaaccccgg
//
LOCUS       DASSHJ010000078         7260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1369962,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000078 DASSHJ010000000
VERSION     DASSHJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369962"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFI43_03240"
     CDS             <1..1062
                     /locus_tag="VFI43_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7061173.1"
                     /translation="VIANDIALMETAAHLVEKLWPDGKRLKPREVVSEFARHLDNELD
                     LMREASNCSQLRRNFLDSPLLLVPEVYWDYCYSGVMVMQRVKGIPISHTEELRRQGID
                     IPKLARVGVEIFFTQVFRDGYFHADMHPGNIFVGSDGRYIAIDFGIMGTLTDEDKNYL
                     AQNFLAFFRRDYKRVAEAHIEAGWAPRNTRVDEFETAIRAVCEPIFDKPLSEISFSRV
                     LLRLFQTSREFNVEIQPQLVMLQKTLLNIEGLGRDLDPNLDLWTTAKPYLENWMADQL
                     GWRGLTRRLRKEASSWAIIMPQYPRLLHQALDEIRSQRIDHKMSEFVKEKRRQNILLS
                     ILVILLTAASLWQLWLFYH"
     gene            1280..2362
                     /locus_tag="VFI43_03245"
     CDS             1280..2362
                     /locus_tag="VFI43_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061174.1"
                     /translation="MALLEIRNVTRRFGSYTAVDNVSISVDAGEFFTLLGPSGCGKTT
                     LLRMIAGFDLPDSGEILLDGKNMVGTPPERRPVHTVFQNYALFPHMTVADNIAFPLRM
                     AGKSAHEIKAKVAEALNNVRLSNFGGRFPHELSGGQKQRVAFARGLVNRPRLLLLDEP
                     LGALDAKLREEMQIELINLQKEVGVTFVFVTHAQDEALALSHRIAVMNRGIVEQVDEP
                     SKIYGFPCNRFVADFIGKISMMDAQVVEAAPLYLRLDVGELGEVTTAGKYGVQTGDRG
                     VIAIRPEQVRISHPSDEPHFTNYFTGRVHDFLYIGDVTTYIVELANGARIEALLPNSA
                     PGRATFFEVGDEVGISWRHDSGVFLQ"
     gene            2359..3291
                     /locus_tag="VFI43_03250"
     CDS             2359..3291
                     /locus_tag="VFI43_03250"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7061175.1"
                     /translation="MKAEEAQRKQQPAKFFVAGPPMLFLLLFFVAPSLIMIVTSFRFP
                     GEFGGLAPFAPSSVPVAPIGTPVETGFTLEAYEFFFGDIIYAKIFLKSFAVAAATTLI
                     CLVMAYPLALLIARSDKRLRNLMVLLVVLPFASNFLIRIYAWMIILGPESALSHFINT
                     FLGAFGLGPVSLLFSPFAVLVGMVYVHLPFMVLPLYTNLEKHDRSLLDAAQDLGAARW
                     QRFWRVTWPLSLPGVFSGSALVFIPVLGMFAVPDILGGTGDILIGNLIKDQFLGTRDW
                     PFGSVLSIMLTLAVLSVAGLAAWFARSSGTERAG"
     gene            3293..4099
                     /locus_tag="VFI43_03255"
     CDS             3293..4099
                     /locus_tag="VFI43_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7061176.1"
                     /translation="MSRGNRWLWLAAILVYAFLYIPLAVVVIYSFNDSRLNAEWVGFT
                     LLWYKTLLNDHEMLLAARNSLIIAFSASLSATLLGTMAGLAIHRYNLKVLPVLVFTPV
                     AMPEILLGVSLLLFFLQVLHLTLGMASIIIAHTTFCVGFVAIIVRARLQGMDESIFEA
                     ARDLGASSWQTFRLVTLPLIMPAVVAGALMSFTLSIDDFVITFFTKGVGEPILPIQIY
                     TMIKVAVTPEVNAVSTLLMLLTLVMIIIASRLEANALADKTSATGGGTGT"
     gene            4096..5238
                     /locus_tag="VFI43_03260"
     CDS             4096..5238
                     /locus_tag="VFI43_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7061177.1"
                     /translation="MTCRTDSRQAGPKSFFRAIPSLLMSLAILFAAGCGNHEKSANSA
                     GGEKVLHLFNWNNYVAPETLDRFSEFCNCRLSQDYYSDNEEMLAKLAAGAAGYDLIVP
                     TGNAMDTLIRQGVLNPLDKSLLPNFKNINPAYLDTAFDPGNKYSVPYAYTITLIGFNR
                     EKLEALDLPTDTWAVIFEPEHLEKIKGRVTVLDSPRELMAAALKYLGYSANDIDEHHW
                     KQAAELILRAKPYWAAFNNTSYVRELAAGNLWLAHGYSNDMFQAAHDAAKTGRRFTIE
                     YSTPKEGAVLALDSMVLHRSGNQSDLAHQFINFMLEGKNAAELTNLVGSGNPNMGAMP
                     YIKPEIANNKAIFPDPELLTRLEMLRDLDRKQRRLLSRLWTEIKLK"
     gene            5327..5638
                     /locus_tag="VFI43_03265"
     CDS             5327..5638
                     /locus_tag="VFI43_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7061178.1"
                     /translation="MSYYHRHVFFCVNQREPGAICCNNHDAQAMRDYAKARVKALKLD
                     GKTKRVRINNAGCLDRCNEGPVIVIYPEDVWYTYVDKEDIDEIIEEHLKNGRVVERLR
                     I"
     gene            5736..6440
                     /locus_tag="VFI43_03270"
     CDS             5736..6440
                     /locus_tag="VFI43_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7061179.1"
                     /translation="MSDSNQNSPASSDTISSQTFFVDGPAGKLETVVDEPGGRSPRGI
                     AVIAHPHPLFGGTMDNKVVYILFKALLEQGFIAAKFNFRGVGRSEGKHEAADNGAGEI
                     DDVLAVVRAVQQQFSSRFDGPAPLVLAGFSFGGAIQAFAAEKLKPQSMVMIAPAVARL
                     NAPPVAYPRDKAGREVAPRILIVHGDQDDVVPLNSVLEWAGPQELPVVVIPGAEHFFH
                     RRLHILKSVVRDFYRS"
     gene            complement(6548..7066)
                     /locus_tag="VFI43_03275"
     CDS             complement(6548..7066)
                     /locus_tag="VFI43_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098609 - cell-cell adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin domain-containing protein"
                     /protein_id="HET7061180.1"
                     /translation="MNKSIKNLRNLLPAIVAAAFLTQAGNLAAQDTSSTESATTSQSV
                     EKKKTQSGAQGDASTIQVPILMFVPLDVSADVENKGCWVKMYDKKNYEGDSLMLTGPI
                     NMPVMVGPFGFNWENKVRSLKTGPKANLTIYDNRNFRDQDKFIQAQSNVPDLSKKMGF
                     FDDFRSMMLSCL"
BASE COUNT         1559 a         1931 c         1987 g         1783 t
ORIGIN      
        1 gttatcgcga atgatattgc cctgatggaa accgctgccc acctggtgga aaagctgtgg
       61 cccgacggga agcggctgaa gccgcgggaa gtggtcagcg agtttgcgcg ccacctggac
      121 aatgaacttg atctgatgcg cgaagcatcc aactgtagcc agttgcggcg caatttcctc
      181 gattcgcccc tgttgctggt tccagaggtc tactgggact actgttattc gggagtgatg
      241 gtgatgcagc gggtgaaagg cataccgatc agccacaccg aggagctgcg caggcaggga
      301 atagatattc ccaagcttgc cagagtcgga gtggaaattt ttttcactca agtgtttcgg
      361 gacggctact ttcacgccga catgcatccc ggtaatattt tcgtcggcag cgacggccgt
      421 tacattgcca tcgacttcgg catcatgggc acgctgaccg atgaggacaa gaattatctt
      481 gcccagaatt ttctcgcgtt ttttcgccgg gactataagc gcgtagcgga agctcatatt
      541 gaagcgggat gggcaccccg taacacgcgc gtggacgagt ttgaaacggc gattcgggcg
      601 gtctgtgagc ccatcttcga caagcccttg agtgaaatct cgtttagccg ggtattgctg
      661 cggctgttcc agacatcgcg ggagtttaat gtcgaaatac agccgcaact ggtgatgctg
      721 caaaaaacct tgctgaatat cgaaggcctg gggcgggatc ttgatcccaa cctcgatctt
      781 tggacgaccg ccaagccgta tctggagaac tggatggcgg atcagctcgg ctggcgaggg
      841 cttactcgcc ggctccgaaa ggaagcatcc agctgggcaa tcataatgcc tcagtatcca
      901 cgcctgttgc atcaggctct ggatgagatt cgctcacaga ggatagacca caaaatgagc
      961 gaattcgtga aggaaaaaag gcggcagaac attctgcttt ccatcctggt gatattgctg
     1021 actgccgctt cgctctggca gctctggctt ttttatcact gaaaatacac tctgcagtat
     1081 gctgccgcat ttgccccgtt gggctgaacg gaatttctcc gtttgagtga tacttgctcc
     1141 ttatagcatc tctttccaag acatcttagc gataagcggg agtccttttc caaggtgttt
     1201 cccgagaccg tcgtccaaag aacagccgcg cgagccggtg accgatattt gctaaaattc
     1261 gcgttttttg tccctagcaa tggcgctact tgaaatccgg aatgtcacac gacgcttcgg
     1321 cagctataca gcggtcgaca acgtctccat cagcgtagat gcgggcgagt ttttcaccct
     1381 gctcgggcct tccggatgcg ggaaaacaac gctcttgcgg atgattgccg gcttcgacct
     1441 cccggattcg ggagagattc tgctggacgg gaaaaacatg gtcgggacgc cgcctgagag
     1501 acgccctgtt catacagttt tccagaacta tgcgctattc ccccacatga cagtagcgga
     1561 caatattgcg tttccgctca ggatggccgg caaatcggct cacgaaataa aggcaaaagt
     1621 tgctgaagct ctcaataacg tgcgcctgtc aaattttggc ggccgctttc cccatgaatt
     1681 gtcggggggg cagaagcagc gcgtcgcgtt cgcgcggggg cttgtcaacc ggccgcggct
     1741 cctgcttctt gacgagccgc tcggcgcact ggacgcaaaa ttaagggagg agatgcaaat
     1801 cgagctcatc aatctccaga aagaggttgg cgtcacgttc gtttttgtga ctcatgcaca
     1861 ggacgaggcc ctggcgttat ctcaccggat agcagtaatg aaccgcggta tcgtggaaca
     1921 ggtagacgag ccttcgaaga tctacggctt tccctgcaat cgcttcgtcg ccgacttcat
     1981 cggcaagatc agcatgatgg acgcacaagt cgttgaagca gcacctttat acctgagact
     2041 ggatgtggga gaactggggg aagttacaac agcgggtaaa tatggtgtcc aaactgggga
     2101 taggggggtg attgcaattc gccctgagca agtgcggata tctcacccat ctgatgaacc
     2161 tcactttaca aactacttca ctggcagggt acacgatttt ttatacatag gggacgtgac
     2221 cacttatatc gtggaacttg ccaatggcgc acggatagaa gcattactgc ccaactcagc
     2281 tccgggtcgt gccacttttt tcgaggtcgg agacgaagtc gggatttcct ggcgtcatga
     2341 ttcaggggtt ttcctgcaat gaaagcggaa gaagcgcaga ggaagcagca gccggcgaaa
     2401 tttttcgtcg cgggccctcc aatgttgttc ctgctgctgt tcttcgttgc tccaagcctg
     2461 atcatgatcg tcacttcgtt tcgctttccc ggcgaattcg gtggcctcgc ccctttcgcg
     2521 ccttcttccg ttcccgtcgc acctataggt acgcccgtag aaacaggctt tacgctcgag
     2581 gcctacgagt tttttttcgg cgacatcatt tatgccaaaa tctttctgaa gtcatttgcc
     2641 gttgcggcgg caacgactct catctgcctc gtcatggcct atccgctcgc gcttttgatc
     2701 gcccgcagcg acaaacgctt gcgcaacctc atggttctcc tggtcgtgct gccattcgcc
     2761 agcaattttc ttattcgcat ctacgcatgg atgatcatac tgggacccga atccgctctc
     2821 agccacttca tcaacacctt tctcggcgca ttcggcctcg ggcccgtgag cttgctgttc
     2881 tcgccatttg cggttctggt ggggatggta tacgtgcatc ttccattcat ggtgttgccc
     2941 ctctatacca acctggaaaa gcatgaccgc tcgcttcttg acgctgccca ggacctcggt
     3001 gcagcgcggt ggcagcgttt ctggagagtc acctggcctc tctcgctgcc gggcgttttt
     3061 tccggttccg cgctcgtatt cattcccgtg cttggcatgt tcgccgttcc ggatattctg
     3121 ggtgggacgg gtgatattct catcggcaac ctgatcaagg atcagttcct gggtacccgg
     3181 gattggccgt ttggctcggt actgtcaatc atgctgacgc tcgcagtttt gtcggtggct
     3241 ggattggctg catggtttgc acgctcatcc ggtacggagc gggcgggcta gcatgagccg
     3301 gggcaatcgc tggctctggc tggcagcaat actggtttac gcgtttcttt acatcccgct
     3361 tgctgtcgtc gtcatctatt cgttcaacga ttccaggttg aacgcggaat gggtgggttt
     3421 caccctgctc tggtacaaaa cgctgctgaa cgatcacgaa atgctgcttg ccgctcgcaa
     3481 ctcgctgatc attgcattca gcgcgagcct ctccgccacg ttgcttggga cgatggcagg
     3541 ccttgccata caccgttaca acctgaaggt attgcctgta ctggtgttta cgccggtggc
     3601 gatgcccgag attctgctgg gtgtttcact gctgctgttc tttttgcaag tgcttcattt
     3661 aacgcttggc atggcttcca tcattattgc tcacaccacg ttttgcgtcg gttttgtcgc
     3721 cattatcgtg cgcgcccggt tgcagggaat ggatgagagt atctttgagg cggcccgcga
     3781 cctcggtgca tcatcctggc agacttttcg cctggtcacg ctgccgctta tcatgccggc
     3841 ggtagtcgcc ggtgcgctga tgtcgtttac cttgtccatc gacgacttcg tcatcacttt
     3901 ttttacaaag ggggtgggcg agccgatcct gcccattcag atctacacca tgatcaaggt
     3961 ggctgtcact cccgaggtaa acgccgtttc gaccctgttg atgctgctga cgctggtcat
     4021 gatcattatc gcttccaggc tcgaagcgaa tgccttggca gacaagactt ccgcgacggg
     4081 tggaggaaca ggcacgtgac gtgccggact gattcacggc aggctgggcc gaagagcttt
     4141 ttcagagcca ttccgtcgct gctgatgtcg ttggcgatcc ttttcgccgc cggctgcggc
     4201 aatcatgaga aaagtgccaa ctcggcggga ggggaaaaag tccttcatct tttcaactgg
     4261 aataactatg tagccccgga aacgctcgac cgtttcagtg aattctgcaa ttgcaggctg
     4321 tcgcaggact actactccga taacgaagag atgcttgcaa aactggcagc gggcgcggca
     4381 ggatacgatc tcatcgtgcc gaccggtaac gccatggata cattgattcg ccagggcgtt
     4441 ctgaatccac tggacaagtc cctgctgccc aatttcaaaa atatcaatcc tgcatatctt
     4501 gacactgctt tcgatcccgg taacaagtat tcagtccctt atgcctatac gattactctg
     4561 atcggcttca accgggagaa actggaagcg ctcgacctgc cgacagatac ttgggccgtt
     4621 attttcgagc cggaacacct ggagaaaata aagggccgcg tcaccgtgct cgacagcccc
     4681 cgcgagctga tggctgccgc gctcaagtac ctgggatatt cagccaatga cattgacgag
     4741 caccactgga agcaggctgc ggagctcatc ctccgggcca agccgtactg ggccgcgttc
     4801 aataacacga gctatgtgag ggaattggcc gcaggtaacc tgtggcttgc ccacggatat
     4861 tcgaacgaca tgttccaggc ggcgcatgat gcagcaaaga caggacgccg ctttaccatt
     4921 gaatactcca ccccaaagga aggagctgtt ctcgcgctcg acagcatggt attgcaccgg
     4981 agtggaaacc agtcagatct tgcgcatcaa ttcatcaatt tcatgctgga ggggaagaat
     5041 gcggccgagt tgacgaatct cgtcggttcg gggaatccga atatgggtgc catgccctat
     5101 atcaagccgg aaatcgccaa caataaggcc atatttccgg accctgaatt gctgacccgg
     5161 ctcgaaatgc tccgtgatct ggatcgcaag caaagacgac tactgagccg gttatggaca
     5221 gagattaaat tgaaatagga acttctgttg agatggagcg tcgcaggaga gcagcggctg
     5281 tttcggtata atcttctcga actcttattc agccattcta gatctcatga gctactacca
     5341 tcggcacgta tttttttgcg tgaaccagcg cgaaccaggg gcgatttgtt gcaataacca
     5401 tgatgcgcag gccatgcgcg attatgcgaa ggcgcgcgtg aaggcgctta aactggacgg
     5461 caagacgaag agggtacgca tcaacaatgc ggggtgcctg gatagatgca acgaaggccc
     5521 ggtcatcgtc atttatcctg aagacgtctg gtatacctat gtagacaagg aagatatcga
     5581 cgaaatcata gaggagcact tgaaaaatgg ccgtgtggtt gaacggctta gaatttaggc
     5641 gtcccgaagt gcagcgcgag ggatccgcga atcgggttgc cccgaggggt gctcgaccgt
     5701 catcgtgccc gtagacttat caagaggttt ccaggttgag cgactcaaac cagaatagcc
     5761 cagccagcag tgatactatt tcctcgcaga ccttttttgt tgacgggccg gcaggaaaac
     5821 tggaaacggt ggtggatgag cccggagggc gttcgccccg cggtattgct gttattgccc
     5881 atccgcaccc gttattcggg ggcacgatgg acaataaggt cgtgtacatc cttttcaagg
     5941 ctttgctgga gcaggggttc attgctgcca aattcaactt ccggggagta gggcgaagcg
     6001 aagggaaaca tgaagcggct gataatggcg caggcgaaat cgacgacgtt ctggctgttg
     6061 tccgcgccgt tcagcagcag ttttcctccc ggttcgacgg ccctgctccg ctggtcctgg
     6121 ccggattctc gtttgggggc gcaatccagg cttttgcagc ggaaaagctc aaaccacaaa
     6181 gcatggtaat gatcgcgccc gccgttgccc ggctgaatgc cccgcctgtt gcctatcctc
     6241 gtgataaagc gggtcgcgaa gttgctcccc gtatcctgat cgtccacggc gaccaggacg
     6301 atgttgtccc gctgaattca gtgctggagt gggccggacc gcaggagttg ccggttgtcg
     6361 tgatcccggg tgctgaacat ttttttcacc gccgcctcca catcctcaag tcggttgtga
     6421 gggatttcta ccgatcctga ccctgccttc aacatactgg aggggaagta aaatttcgcg
     6481 ggcattcatc ccagaatttc ctgatttata accgctcaat ggcttccctc tgcttgcctt
     6541 tccactctta caagcagctc agcatcatcg atctgaagtc atcgaagaac cccattttct
     6601 ttgatagatc cggtacgttt gactgtgcct ggataaactt gtcctgatcg cgaaaattac
     6661 gattgtcata aatggtcagg tttgccttgg gccccgtttt caggctgcgc accttgtttt
     6721 cccaattgaa accaaagggg ccgaccatca cgggcatatt gattgggccc gtaagcatca
     6781 agctgtcccc ttcgtaattt ttcttgtcgt acatctttac ccagcatccc ttgttttcta
     6841 cgtcagctga aacgtccagc gggacgaaca tcaatatcgg gacctggata gtcgaggcgt
     6901 cgccctgggc tccggactgg gtctttttct tctcaactga ttgagatgta gtcgctgact
     6961 ccgtgctcga agtgtcctgg gcagcaaggt tgcctgcttg agtcagaaac gcggcggcaa
     7021 caatcgcggg caggagattg cgtaggtttt ttatcgactt gttcatgtca cactccttat
     7081 atggaagaga aatataatgt acaaaatacc ggcaaaaggc agagctgacg tggcacgtat
     7141 tcactccgcc tggccgatcc ggggggcact cgggtatcct gtcccagagt gctaaataca
     7201 attcaatatc atcgagcgga agttatcgaa caaccccatt ttcttggata gatttggaat
//
LOCUS       DASSHJ010000079        11539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_606723, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000079 DASSHJ010000000
VERSION     DASSHJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606723"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFI43_03280"
     CDS             <1..199
                     /locus_tag="VFI43_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7061181.1"
                     /translation="MANIGGRAAGTITAACFLWRFTKKYRWAHLDIAGTAWKSGKEKG
                     STGRPVPLLTQFLIERASRNS"
     gene            202..615
                     /locus_tag="VFI43_03285"
     CDS             202..615
                     /locus_tag="VFI43_03285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HET7061182.1"
                     /translation="MTKIDFYSGGGNRLSIACRLVAKAVKMRCKVMIYTPDSSMIEQL
                     DTLLWTVPPTDFIPHCRVDDRLADVTPVLLGYQSAKMPHDEILLNLDLENPSFFSRFR
                     RLIEIAGSQEKEVEAARKRYRFYQDRGYEIQHHKL"
     gene            709..1071
                     /locus_tag="VFI43_03290"
     CDS             709..1071
                     /locus_tag="VFI43_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061183.1"
                     /translation="MKKDTKMVHETEYDEIVRKLDALLSKHKHPSPGVTEPITPAAPF
                     SAAPADKAEAPLAATDNIPTLTEIVQVAPAMLSPHADIRSLLYQILDSALKDTGVDLD
                     AATRSRLVQALESQLFSL"
     gene            1213..4029
                     /locus_tag="VFI43_03295"
     CDS             1213..4029
                     /locus_tag="VFI43_03295"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7061184.1"
                     /translation="MELEKSFNPRAIESNWYSAWEAAAYFKPAPSTDGTPGASAFCIM
                     LPPPNVTGTLHMGHAFQHTLMDTLIRYHRMCGDNTLWQPGTDHAGIATQIVVERQLDQ
                     QGIDRRDLGREEFLERVWEWKAESGSTITRQMRRLGASCDWSRERFTMDEGLSRAVTE
                     VFVRLYNEGLIYRGKRLVNWDPVLQTAVSDLEVVSVEEEGALWHVLYLLESDLPEKTG
                     DAVRAQAEPEGVIVATTRPETMLGDMAVAVHPDDLRYKHLIGRHVRLPLAERTIPIIA
                     DSYVDPEFGTGCLKITPAHDFNDYQVGKRHNLVPLNIFTLDGKINDLAPVEYQGLDRF
                     DARKKIISDLEQRGLLSSIKPHKLMVPRGDRTHAVIEPMLTDQWYVSMEGLAKSGLEA
                     VASGNIQFIPGNWIHVYDQWLENIQDWCISRQLWWGHRIPAWYDEDGNITVAHSLEEA
                     QQQAGTLKLRQDEDVLDTWFSSALWPFSTLGWPEETPELRTFLPTSVLITGFDIIFFW
                     VARMVMMSLHFTRKVPFREVYITGLIRDAEGHKMSKSKGNVLDPLDLIDGVTLPELIL
                     KRTTGLMNPKQAESIERTTRKQFPNGIPAFGADALRFTFASLASHGRDIKFDMQRCEG
                     YRNFCNKLWNATRYVLMNCAEQKTAPQEPISSHDTRTPLQFSDADKWIISRLQHAERA
                     VRQAFQDYRFDIAAREIYEFVWDEYCDWYLEFAKVQLSSGAEPMQHATRRTLIMVLEA
                     TLRLAHPVIPFITEELWQHVAPLAGKNGKSIMIEPYPDADSKKFDEDASRRIAALKEM
                     VNACRTLRGEMNLSPAIKVPLLAEGNKQILMQFTPYLMGLAKLSAVEIMPGELPDAEA
                     PVAIVGESRLMLKIEINVDEERARLTKEAARINSEIAKAESKLRNPSFTERAPTAVVQ
                     QEKERLAGFSATLGKVQEQLRKLE"
     gene            4209..4862
                     /gene="adk"
                     /locus_tag="VFI43_03300"
     CDS             4209..4862
                     /gene="adk"
                     /locus_tag="VFI43_03300"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7061185.1"
                     /translation="MIIILLGGPGAGKGTQANFLRERFGIPQISTGDMLRAAVKAGTE
                     LGIMAKKIMDAGGLVSDEIIIALVKARIAQPDCRRGFLFDGFPRTIPQADAMKAAGVP
                     IDFVIEIDVSDEEIVRRLSGRRVHPGSGRVYHVLFNPPRTDNRDDVTGEPLVQRDDDR
                     EETIRKRLEIYHGQTEPLVEYYARWSAHGGKGAPKYVKIAGIGSVENIRDAIFASLK"
     gene            5068..6330
                     /locus_tag="VFI43_03305"
     CDS             5068..6330
                     /locus_tag="VFI43_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7061186.1"
                     /translation="MAAKNAFYAQSGGVTAVINASASGVIETARGQEGKIGKVYAGRN
                     GIIGVLTEDLIDTSQDSAEAIRALRYTPAGAFGSCRYKLKSLEQNRREYERLMEVFKA
                     YDVGYFFYNGGGDSADTCLKVSHLSDTLGYPVQAIHVPKTVDNDLPITDCCPGFGSVA
                     KYIAVSTLEASFDVASMSKTSTKVFVLEVMGRHAGWIAAAGGLASRKNDEIPVVILFP
                     EITFDKTKFLGKVDSCVKEFGYCSVVVSEGVKGADGKFLADQGLRDAFGHAQLGGVAP
                     VVANIVKDGLGLKYHWGVADYLQRAARHIASRTDVEQAYAMGKAAVEFAIAGHNSVMP
                     TVERVSNSPYQWKVGMAQLSEVANVEKMMPKEFITEDGFGITDKCREYLTPLIGGEDY
                     PPYTEDGLPDYVRLKNVPVPKKLPDFAL"
     gene            6443..8476
                     /locus_tag="VFI43_03310"
     CDS             6443..8476
                     /locus_tag="VFI43_03310"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET7061187.1"
                     /translation="MGNALIIAIGCAMVALVYGALSIKWIVALPTGNERMRDIALAIQ
                     QGASAYLNRQYTTIGIVGVILLIAIFLALGWQTAIGFALGAFLSGLTGYIGMNVSVRA
                     NVRTAEAARHGLNAALDVAFKGGAITGMLVVGLGLLGVAGYFALLTGMGETESQATHA
                     LVGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDCAGMAADLFETYAVTIIATMLLGGLLITGEGSNAVLYPLVLGGVSILASVIGCYF
                     VKAREGGKIMNALYRGLAVAGVLAAIAYYPVTTIMLGDGVMIEGKLVSTMNIYLSVLV
                     GLALTAAMVWITEYYTSTEYAPVRHIAQASSTGHGTNVIAGLGVSMKSTAYPVMVVCL
                     SIWITFELAGLYGIAIAATSMLSMAGIIVALDAYGPITDNAGGIAEMAGLPPEVRDIT
                     DPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALSSGGKVVNFDLSDHMVIIGLF
                     LGGMVPYLFGAMAMEAVGRAAGSVVVEVRRQFKEIPGIMEGTAKPDYSRAVDMVTKAA
                     IKEMIVPSLLPVAVPVLVGVLLGPVALGGVLIGAIITGIFVAISMTAGGGAWDNAKKY
                     IEDGNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAINPLIKIMNIVALLIVPLI"
     gene            8598..9008
                     /locus_tag="VFI43_03315"
     CDS             8598..9008
                     /locus_tag="VFI43_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7061188.1"
                     /translation="MNNKINLTLDDAKTISAGSEAEARRNNWPVVIAIVDDGGHLIHL
                     IRLDNTQFGSVEVAIGKARSAIAFRRPTKVWEDQITEGRLRYLGLPGALPVEGGLPIE
                     ASGQFIGAVGVSGVRSHQDAQIARAGVEAWVNSR"
     gene            9260..10318
                     /locus_tag="VFI43_03320"
     CDS             9260..10318
                     /locus_tag="VFI43_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7061189.1"
                     /translation="MNIPELQQKAFLLMLAAVSVAFAWILLPFYGAVFWGSVLAIIFT
                     PFYRKLLNLMNQRQNLAALTTLLLCLIMVILPLTFITASLLREGLIVYQRIRSGELNF
                     GEYFQQIMNALPHWVVDLMERSGIGNLTQLRDALSNSILQGSQFIATEALSIGQNTFE
                     FVVSFFIMLYLLFFLLRDGAALTAKINKAIPLSMEHKHHLISKFTTVVRATVKGNIAV
                     AATQGVLGGLIFWFLDIQGALLWGFVMAILSLLPAVGAGLIWLPVAVYFLLTGAIWQG
                     VVLILFGVLVIGLVDNVLRPLLVGKDTQIPDYVILISTLGGMVLFGLNGFVIGPVIAA
                     LFIAAWDLFATASDNSRG"
     gene            complement(10339..11154)
                     /locus_tag="VFI43_03325"
     CDS             complement(10339..11154)
                     /locus_tag="VFI43_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7061190.1"
                     /translation="MNEKIALVTGASSGIGRATARLLAKNGYYVFAVARRMHRLEQIR
                     SDRIEPVMLDVSDAEGIQATIDHIISTKGRIDVLVNNAALCQLGAIECVSMDAAHHQF
                     EVNVFGYARFMQAVLPHMREQKSGCIINIVSALSRVSLPGFGWYAASKHAIEALTDAL
                     RGEVMEFGIEVVLIAPGLIKTEFVPNQLRLLQKVAHPPAYRKLLAGVRRLLADEPKAP
                     GPDIIAQSVLQAVKARFPPVRHALPFDAKLAVIARWLLGERLFAWGARQQMKL"
BASE COUNT         2575 a         3099 c         3245 g         2620 t
ORIGIN      
        1 catggcgaat attggcggca gagcggccgg aaccattacc gccgcatgct ttctctggcg
       61 ttttaccaag aaataccgct gggcccattt ggacatagcc gggactgcat ggaaatccgg
      121 caaggaaaaa ggctctacgg gtcgccctgt cccattgctt acccagtttt taattgagcg
      181 cgcatcccgc aatagctagg gatgacgaaa attgactttt actccggtgg aggaaatcga
      241 ttatccatag catgccggct cgttgcgaaa gcagtgaaaa tgcgttgcaa ggtgatgatt
      301 tatacacctg attcaagcat gatcgaacag cttgacacgc ttctgtggac agtgcctccc
      361 actgatttta ttccccactg ccgcgtcgac gacagattgg ccgatgtaac ccccgtcctg
      421 ctgggctatc aatctgcaaa aatgccccac gatgagatct tgctcaatct tgaccttgaa
      481 aacccctctt ttttcagccg cttccggcgc ttgatcgaaa tcgcgggaag ccaggagaaa
      541 gaggtggagg cggcgcgaaa gcgctaccgg ttctatcagg atcgcggtta tgaaattcag
      601 caccacaaac tttagctccg ctgattgata gacatacagc tggcttcatc gttgatgagg
      661 ccatcgggta aagtccattc ttctctccac aacattcttg gaagctcgat gaaaaaagat
      721 accaagatgg tgcacgaaac ggaatacgat gaaattgtgc gcaagctgga tgccctcctg
      781 agcaagcaca agcacccaag cccgggcgtg acggagccga taactccggc cgctcctttc
      841 tcagccgcac cagcggacaa ggcggaagca cccctggcag cgacagacaa tattccgaca
      901 ctgaccgaga tcgttcaggt tgcccccgcg atgttgtctc cgcatgccga tattcgatca
      961 ctcctctacc aaattctgga ttctgcgctg aaagacacgg gagttgatct ggatgcggca
     1021 acacgaagca gactggtaca agcgctggaa tcacagctgt tcagcctttg agcaattcat
     1081 agcggtgcca tgggaaccta ggcttggttc aaacgcgcct cgggctccat cgcatcggtg
     1141 accctccctc gtccccactt attcctgtat tcattccgct ataattctgc ggtttagcgc
     1201 aaacccgtcc tcatggaact cgaaaaaagc ttcaatcccc gtgcaatcga aagcaactgg
     1261 tattccgcct gggaagcagc ggcgtatttc aagccagctc ccagcactga tggcaccccg
     1321 ggcgcctctg cgttctgcat tatgctgccg ccaccaaacg tgaccggtac cttgcatatg
     1381 ggacacgcct ttcagcatac cttgatggat acattgatac gctaccatcg catgtgtggt
     1441 gacaatacgt tgtggcaacc agggacggat cacgccggga ttgccaccca gatcgtggtg
     1501 gaacgccagc ttgatcaaca aggtatcgac cgccgcgacc tgggccggga agaatttctc
     1561 gagcgggtat gggaatggaa ggcggagtcc ggctccacca ttacccggca gatgagacgg
     1621 ctgggcgcct cctgcgactg gtcgcgtgag cgtttcacga tggatgaagg gctctcgcgc
     1681 gcggtgaccg aggtttttgt gcgcctctac aacgaaggcc ttatctaccg cggcaaaagg
     1741 ctggtcaatt gggatcccgt cttgcaaacc gctgtttccg acctcgaagt ggtttcagta
     1801 gaggaagagg gggccctctg gcatgtcctt tatctccttg aaagtgacct ccctgaaaaa
     1861 accggagatg ccgttcgtgc ccaggctgaa ccggaaggag tgattgttgc gacgacgcga
     1921 cccgaaacca tgctgggcga tatggcggtg gcagtccatc cggatgactt gcgctataaa
     1981 catctgatcg gccgccatgt ccggctgccg ttggcagaac gaacgattcc gataatcgcc
     2041 gatagctacg tcgatcccga gtttggcacg gggtgtttga aaatcactcc tgcccatgat
     2101 tttaacgact accaggtagg aaagcggcac aacctggttc ccctgaatat tttcaccctg
     2161 gatggaaaga tcaatgatct ggcgccggtg gaataccagg ggctcgatcg ttttgatgcc
     2221 cgcaaaaaga tcatttccga cctggaacag cggggcctcc tgtcgagcat taaacctcac
     2281 aagctgatgg tgcccagagg tgaccgtaca cacgcggtaa tcgagcccat gctgacggat
     2341 caatggtatg tctcaatgga aggtctcgcc aaaagcggtc ttgaagccgt ggcgagcgga
     2401 aacatccaat tcattcccgg aaactggatc catgtgtatg accagtggct ggagaatatc
     2461 caggactggt gcatttcccg ccaattatgg tgggggcacc ggattcccgc ctggtatgac
     2521 gaggatggga atataaccgt tgctcacagc ctggaggagg cgcaacagca ggcgggcaca
     2581 ttgaagctga ggcaggatga ggatgtgctc gacacttggt tttcgtccgc gctctggccg
     2641 ttctccacac tcggctggcc cgaggaaacg cccgaactcc gtaccttcct gccgacttcc
     2701 gtattgatca caggattcga catcattttc ttctgggtgg cacgcatggt gatgatgtcg
     2761 ttgcatttca cccgtaaagt tccgttccgg gaagtctaca ttactgggct gatccgcgat
     2821 gccgaaggcc acaagatgag caaatcgaaa ggtaacgtgc tcgacccgct ggacctcatc
     2881 gacggcgtca ccttgccgga attgatcctt aagcgtacga ccggcttgat gaatcccaag
     2941 caggcggagt ccatcgaaag aaccacgcgg aagcaatttc cgaacggaat tcccgcgttt
     3001 ggcgcagacg cactgcgttt cacctttgca agcctcgcct cccacgggcg cgacatcaag
     3061 ttcgacatgc agcgctgcga gggctaccgg aatttctgca ataaattatg gaatgcgacg
     3121 cggtacgttt tgatgaattg cgcggagcaa aaaaccgccc cgcaagaacc gatcagctcg
     3181 cacgacaccc gcacgccgtt gcaattttcc gacgcggata aatggatcat cagccggctg
     3241 caacatgcgg aacgcgcggt gaggcaggct ttccaggatt atcgcttcga tattgcagcg
     3301 cgcgaaatct atgagttcgt ctgggacgaa tactgtgact ggtacctgga gttcgccaag
     3361 gtacagttga gttcgggcgc ggagccgatg cagcacgcta cccgccgtac gctgataatg
     3421 gtactggaag caacgctgcg gctcgcgcat cccgttattc cgtttatcac tgaagaacta
     3481 tggcagcacg tcgcgcccct tgccggtaaa aatggtaaaa gcatcatgat cgagccctat
     3541 cctgatgccg actcgaaaaa atttgacgag gacgcgtctc gccggattgc tgcattgaag
     3601 gaaatggtca atgcatgtcg gacgcttcgt ggcgagatga acctgtcgcc ggcaatcaaa
     3661 gtacctttgc ttgcagaagg gaacaagcag attcttatgc aatttacccc atacctgatg
     3721 gggttggcga agctgtcggc agtggaaatt atgcctggtg aattacccga tgccgaagcg
     3781 cctgtcgcaa ttgtgggcga gtccaggctg atgctgaaaa tcgaaatcaa tgtcgacgag
     3841 gaacgcgcgc ggctgacgaa ggaggcggcc cgtatcaaca gcgaaatcgc caaagccgaa
     3901 agcaagctgc gcaacccgag ttttacggag cgcgccccta cagcagtcgt gcagcaggaa
     3961 aaagaacgtc ttgccggctt cagcgcaacc ttgggtaaag tgcaggaaca gttgcgcaag
     4021 ctggaatgaa gcaggctaaa aatgaatact tcaggttgcg gagcggaaag tgacgtcaga
     4081 tgaataaaaa gcgtcggtaa ataattcaag catctagctg ggctgcgtta taattaccgg
     4141 aaatagcgaa caaatactga ctctctgctt actttttcag cagagatgta ttgaagagaa
     4201 gggaaagaat cataatcatc ctgttagggg gccccggagc gggtaaaggc acccaagcca
     4261 attttttaag ggaacggttc ggcatccctc agatttcgac cggggatatg ctccgcgccg
     4321 cggtaaaagc cggtactgag cttggcatca tggcgaagaa gatcatggat gcgggaggtc
     4381 tcgtatccga cgagatcatc attgccctcg tgaaagcgcg aattgcccaa ccggactgca
     4441 ggagaggctt cctgtttgat ggcttcccaa gaaccattcc tcaggcagac gcgatgaaag
     4501 cggccggtgt gcccattgat tttgtcattg aaatcgacgt atccgatgag gagatcgtac
     4561 gccgcctgtc gggcaggaga gttcatcccg gctcggggcg ggtttaccac gtgctattca
     4621 atccgcccag gacggataac agggacgatg tcacgggcga accgctggtc cagcgcgacg
     4681 atgacaggga ggaaacgata agaaaacggc tggagatcta ccatggccag actgaaccct
     4741 tggtagaata ctatgccaga tggtccgctc atggcggaaa gggcgcccca aagtacgtga
     4801 aaattgcggg gatcggatcg gttgaaaata tccgtgatgc cattttcgcg tcactgaaat
     4861 aaccttcgat caaaattcct ggcgggacgc gttgtgtttc cagcgttacc cgccctttaa
     4921 attcgcgaac aagcaaacaa tacgggccgg tagcgcggct gaggcgttgc cgttgcgcgt
     4981 tgcggtcgtt agactagcgc cccggggaga agggcttgcc ggttctcgcc tggggtaaca
     5041 gaggattctt attatcaggg gagaaatatg gcagcaaaaa atgcgtttta tgctcaatcc
     5101 ggcggggtta ccgccgtcat aaacgcttcc gcctccgggg ttatcgaaac tgcgcggggg
     5161 caagaaggaa aaataggaaa ggtctatgcg ggccgcaacg ggattattgg ggtgctgacc
     5221 gaagacctga ttgataccag ccaagactct gccgaagcca tcagggcgct gcgctacact
     5281 cctgcgggcg cgttcggctc ctgccgatac aagctcaaaa gcctggaaca gaaccgccgc
     5341 gagtatgagc ggctgatgga agtattcaag gcgtacgacg tcggctattt cttttacaac
     5401 ggaggcggcg attcggcgga cacctgcctt aaggtatccc atctgtccga taccctgggt
     5461 taccccgttc aggccatcca tgtgccgaaa accgtggata acgatcttcc catcacggac
     5521 tgctgccccg gtttcggttc agtcgcgaaa tacattgccg tttcgacgct ggaagcaagc
     5581 tttgatgtgg cgagcatgtc gaaaacgtcg accaaggtat ttgtccttga agtgatggga
     5641 cgtcatgccg ggtggattgc ggcagccggc ggcctggcat cccgaaagaa cgatgaaatt
     5701 cccgttgtga tcctcttccc ggagattacg ttcgacaaaa ccaaattcct cggcaaggtt
     5761 gatagttgcg tgaaagagtt cggctactgc tcagtggtcg tgtcagaggg agtgaagggc
     5821 gcggatggaa agttcctcgc tgatcaggga cttcgtgatg cattcggtca tgcgcaactc
     5881 gggggggtcg cgccggtggt ggccaacatc gtcaaggacg ggctggggct caagtatcac
     5941 tggggcgtgg cggattactt gcagcgggcc gcgcgtcata tcgcgtccag gacagatgtt
     6001 gagcaggcgt atgcaatggg caaggcggcc gtggagttcg caatcgctgg acacaactcc
     6061 gtcatgccga cggtggagcg cgtttccaat tcgccttacc agtggaaagt cggaatggcg
     6121 cagctatccg aggtcgccaa tgtcgagaaa atgatgccga aggaattcat caccgaggat
     6181 ggctttggaa ttaccgacaa gtgccgggaa tatctgactc ccctcatcgg cggggaagat
     6241 tatcctcctt acaccgaaga tggcctgccc gactatgtgc gcctgaagaa cgtgcccgtg
     6301 cctaaaaagc tgccggactt tgccctctga gtgtccacca ggtgcagcgg gaattatatc
     6361 tgtcgtcgtt ctgtttacca tgaaaccatg agtctggatt cttgccgggt tcaggcaata
     6421 tgaccttaaa ctggagattt tgatgggtaa tgctttaatc atcgcgatcg gttgtgcgat
     6481 ggtagcgctc gtgtatggag cgctgtcgat aaaatggatt gtagccctgc cgacgggcaa
     6541 tgagcgtatg cgtgatattg cgcttgccat tcagcaggga gcttcggcct atctgaaccg
     6601 tcaatacacc acgatcggca tcgtgggcgt gattttgctg attgccatct tcctggccct
     6661 gggttggcag acagctatcg gtttcgcgct gggcgcattc ctgtcgggat tgacgggcta
     6721 tataggaatg aacgtatcgg tgcgcgcgaa tgtccgcact gccgaagctg cccggcatgg
     6781 cctcaatgcg gcgctggacg tagcattcaa gggtggagcc attacgggaa tgctggtggt
     6841 gggattgggc ttgcttgggg tagctggtta cttcgcccta ctgaccggga tgggcgaaac
     6901 ggagtcccag gcgacccacg cgctggtggg gcttgctttc ggaagctctc tgatttcaat
     6961 ttttgcccgt cttggcggcg gcatttttac caaaggtgct gacgtgggag cggacctggt
     7021 tggtaaggtt gaagcgggaa ttccggaaga cgacccgcgc aatcctgcag tgattgccga
     7081 caacgtcggg gataacgtcg gggattgcgc tggaatggct gccgacctgt tcgaaaccta
     7141 cgccgttacc attatcgcca ccatgctgct gggcggcctg ctgatcaccg gagagggttc
     7201 gaacgcggtg ctctacccgc tggtacttgg gggcgtctcc attctggcct cggtcattgg
     7261 atgctacttc gtcaaggctc gcgagggtgg caagatcatg aacgcgttgt atcggggact
     7321 cgcggttgcc ggtgtgctgg ccgctattgc ctactatcct gtcacaacca taatgctcgg
     7381 tgatggggtg atgatcgagg gcaagctcgt ttccacgatg aacatatacc tttccgtcct
     7441 tgtcggcctc gccctgaccg cagcgatggt ctggattacc gagtactaca cctcgaccga
     7501 atacgctccg gttcggcata ttgcccaagc gtccagcacg ggccacggca cgaatgtcat
     7561 tgcaggcttg ggagtgtcca tgaaatccac tgcctatccc gtgatggtcg tctgcctatc
     7621 gatctggatt accttcgaac tggcggggtt gtatggaatc gccatcgccg ctacatcgat
     7681 gctttcgatg gctggcatca tcgttgcgct tgacgcttac ggccctatca cggataacgc
     7741 cgggggcatt gcggaaatgg ccggcttgcc gcccgaagtg cgcgatatta ccgatccgct
     7801 cgatgccgtc gggaacacga ccaaggcagt gaccaagggc tacgcgatcg gttcggccgg
     7861 tctggcggcg ctcgtcctgt tcgccgatta cacccatgcg ctctcgagcg gcggcaaggt
     7921 agtgaacttc gatctgtccg atcacatggt gatcatcggc ctctttctcg gcggcatggt
     7981 gccttacctg ttcggagcga tggcaatgga agcggttggc cgcgccgcag gctcggtggt
     8041 cgtggaagtg cgccggcagt tcaaagagat accagggatc atggaaggta cggccaagcc
     8101 tgattactcg agggcggtgg acatggtcac caaggctgcg atcaaggaaa tgatcgttcc
     8161 ctcgttgctg ccggtagcgg ttccggtgct tgtcggtgtt ctgctcggtc ccgtagcctt
     8221 gggcggcgtg ttgatcgggg ctatcataac gggcattttt gtggccattt ccatgaccgc
     8281 tggcggcgga gcatgggata acgcgaaaaa atacatcgag gacggcaact tcggcggcaa
     8341 gggctcggag gcgcataagg cggccgtaac cggagatacg gttggtgatc cctacaagga
     8401 taccgcgggc cccgccatca acccgctcat caagatcatg aatatcgtcg cattgcttat
     8461 cgtgccattg atataaccat ccttgagttc ccgaagcatg ccggcctcct ggcatgcttc
     8521 ttttttaagc agatcatgtg cacgatgatt tgaattgaca agccgctgat ttttagcagc
     8581 gattctctat taacggcatg aacaacaaaa ttaacctcac gcttgacgac gccaaaacca
     8641 tctcggcggg atccgaagca gaggccaggc ggaacaattg gcccgtcgtt atcgccattg
     8701 tcgatgatgg aggccacctt attcacttaa tccgcctaga caatacgcaa tttggaagcg
     8761 ttgaggttgc gatcggaaaa gcgcgctccg ccatcgcctt ccgtcgtcct accaaggtat
     8821 gggaggatca gattacagaa ggccggctgc gttatctcgg gctgccggga gcgcttccag
     8881 tcgaaggggg cctgcctatc gaggccagcg gccaattcat cggggcagtc ggcgtaagcg
     8941 gcgtgagatc tcaccaggat gcacaaatcg cccgcgctgg ggtggaggca tgggtcaatt
     9001 cccgctagtg ccctttccac ttcgagtcag accagaaccg gacgaactct gcgcaattca
     9061 cgaaactctt ctggcgcttc gccgaaagat cgggagtgcg attggatgtt gcagagtccg
     9121 ggaatatccg cccgtcccca ttctcttttc gcaggccctg ctcttttcgc aggccctggc
     9181 ctactcaagg tcaacttgag gtacgattga gcatgggcga agtaaagtcc tctcgcctcc
     9241 cctcacctgc tgtccgagca tgaacatccc cgagcttcaa caaaaagcct ttcttctcat
     9301 gctggccgcc gtttccgtgg cgtttgcatg gatactgctg cccttttacg gggctgtatt
     9361 ctggggttcg gttctggcga tcatatttac ccctttttac cgcaagctgc tgaacctcat
     9421 gaatcagcgc cagaaccttg cggcgctcac caccctgctg ctctgcctca tcatggtaat
     9481 ccttccgttg acgtttatca cggcttcttt actgagggag ggattgatcg tttatcaacg
     9541 aatccgctca ggagagttga atttcggcga gtactttcag caaatcatga atgccctgcc
     9601 acactgggtc gtcgacctga tggaacgttc gggcataggc aatcttactc aacttcgaga
     9661 cgcgctctcg aacagcatcc tccagggaag ccagttcatt gcgaccgaag cgctgagtat
     9721 cgggcagaac acattcgagt ttgtggtcag cttcttcata atgctctact tgctcttttt
     9781 tttgctacgg gatggtgcgg ctttaaccgc caagataaac aaggcgatac cgctgagcat
     9841 ggagcacaag catcacttga tcagcaaatt cactaccgtc gttcgggcga cggtaaaggg
     9901 caacatcgcc gtagctgcca cccagggtgt cctgggtgga ctcatattct ggtttcttga
     9961 tattcagggc gcactcctct gggggttcgt catggcgatc ctctcgctgc ttcccgccgt
    10021 aggcgctgga ttgatctggc taccggtggc agtctacttt ttacttaccg gagcgatctg
    10081 gcaaggcgtc gtccttattc tcttcggcgt attggtcatc ggactggtcg ataacgtttt
    10141 gcggcctcta cttgtgggca aagacactca gatacccgac tatgtgatac tcatttcaac
    10201 acttggcggc atggtgcttt tcgggcttaa tggttttgtc atcggtcccg tcattgcagc
    10261 actgtttatt gcagcatggg atctttttgc cacagcaagc gataacagca ggggttagac
    10321 ttttcggctt ctgacacgtt acaacttcat ttgctgcctc gcaccccatg caaaaagtct
    10381 ctcgccgagc aaccagcgcg cgataacggc aagttttgcg tcgaaaggca gggcgtgacg
    10441 cacgggcgga aaccgcgcct tcaccgcctg gagcacggac tgagcaataa tatccggccc
    10501 cggcgccttc ggctcgtctg caagcagtct gcgaacccct gccaacagct ttctataagc
    10561 gggaggatgg gccaccttct gcaacaatct caactgattg ggtacaaatt cagtcttgat
    10621 gagtcccggc gcgataagca cgacttcgat tccaaactcc atcacttcac ctcgcaacgc
    10681 gtccgtcaat gcttcgatag catgcttcga ggctgcatac cagccaaacc ccggaagcga
    10741 taccctgctg agggcggaaa cgatattaat gatgcatccc gacttctgct ctctcatgtg
    10801 cggcaaaacc gcctgcatga agcgcgcata tccgaaaaca ttcacctcga attggtgatg
    10861 cgccgcgtcc atggaaacgc attcgatggc accgagctga cagagcgcag cattgttcac
    10921 gagcacatcg attcttccct ttgtcgaaat gatgtgatcg atggtcgcct ggataccttc
    10981 agcgtcagac acatcgagca taaccggttc gatgcgatcg gagcgtatct gctccagcct
    11041 gtgcatccgg cgggcgaccg caaagacgta atatccattt tttgccagaa gtcgtgcagt
    11101 cgcccttcct atgccactgg acgcccctgt caccaatgcg atcttttcgt tcatcttctt
    11161 cctcctcgag atttctttac gtcgtgattt ccttgggtct gcttgcgctg acgtccattt
    11221 ggagaaaata ccgctggcat tgatgagacc ggatttgaaa cgttggtatt tggcatatcc
    11281 ccagctgcgg ccgttgttcc gtttaattat tggcgcttct cgttatgtat tcagaatgac
    11341 caggcataat ccagatcgga tttccggttt ctctttaacc gaagctgctc tttccggcag
    11401 cctttcccgc tctgtctata attctagggc ctgccgtccg aacagggaac cgtacagcat
    11461 agagaagcaa cgacgaaatt gtaacgaagg ggaaccatcc gggaggttac atgaatacga
    11521 cattgatcac gggagcaaa
//
LOCUS       DASSHJ010000080        15191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_667015, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000080 DASSHJ010000000
VERSION     DASSHJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667015"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFI43_03330"
     CDS             <1..201
                     /locus_tag="VFI43_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length-determining protein"
                     /protein_id="HET7061191.1"
                     /translation="FAISQIRPTFHSQVSLREATGRPILGSIPMIWTSAEKIKRRKRL
                     YAFGISLLSLLGLYGALMVKMA"
     gene            415..1362
                     /locus_tag="VFI43_03335"
     CDS             415..1362
                     /locus_tag="VFI43_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HET7061192.1"
                     /translation="MSIIERAAGQLGAEKQAGPERISQTGHLNPVQKSSHSGEVETTV
                     EQAIHADSDQATRPAGSRTGMSQGPAQTTRRIILNQEKMRQYGIVTPDQGRTQIAEQF
                     RVIKRPLLTNAFNRGPGMIKNGNLIMVTSALAGEGKSFCTVNLAMSIAMEMDRTVLLV
                     DADVARPTLPRILGLGTERGLLDILLDEKLELADVLIKTDIEKLTLLTAGRRHSHSTE
                     LLASQSMNVLLKELAERYADRVVIFDSPPLLLTSEARVLATQMGQIVLVVEAERTSQQ
                     AVKETLRQIESCDVVNLIYNKARSFPGGEYYGYYYHESA"
     gene            1349..2917
                     /locus_tag="VFI43_03340"
     CDS             1349..2917
                     /locus_tag="VFI43_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HET7061193.1"
                     /translation="MKAPDPRCRKSSFPVTFPAVVAIFLLSSNADASEWKIRPRMNLI
                     ETYSDNLRLRSGSGSDDFITQINPGILVNGVASRYTVDVNYLMNNLIYAQNGNFNRIQ
                     NQLNANGTGELIKDLFFVDGRAFITQQNVSLLGPQAINNVNVTGNRTNVSTYVLSPYL
                     RHRFQNFATTELRYTRNWVNSSTNGLASSTGDGVLFNLNSGTAFKTFGWGVNYSNQMI
                     HFDSGRDIELERSTGTLRYMLTPQFGLNATGGYERNSFVSIRGSPSAPLWTVGFVWEP
                     TQRTNISVSGGQRFFGNTYNALASHRTRLMVWSFGYNQNITTFNQQSAFGTGIPGSLG
                     GSLNQLLTAQNPNLSPDVIQQNTAALTGIGLSGSFFNPMNFLTNRLFLQKTLQASVVM
                     NGSRNTITASLFNMTRQAYSDPLADINLVGAQNLALLNHTRQSGVNALWNYRLSERTS
                     ANLNFAYTRFKFLGAGREDDLMLVMLGLTRQFRSIQPNLSGTLQVRHQQRDSNQPGSD
                     YREQAVIGSLNMSF"
     gene            2927..3931
                     /locus_tag="VFI43_03345"
     CDS             2927..3931
                     /locus_tag="VFI43_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HET7061194.1"
                     /translation="MYPSFYGFRVKPFKLKPDPDFYFGSKGHKRAMAYLEYGLAQGEG
                     FIVITGDVGAGKTTLVRYLFRKLDSKEIVAAHIANTDLDSGDTLRMVASAFGLPFENL
                     SKASLLISIEQFLQACDQQGKRALLVVDEAQNLSPRTVEELRMLSNFQTEEKPLLQTF
                     LLGQPEFRKTLLGADMQQLRQRVTATYHLGPMDLPETRAYIEHRLRTAGWQGDPAFKD
                     GAFEAIYGSSGGIPRKINALCDRLLLMGYLEKLHEFGEEEVNEVINDIQQEFEVAITP
                     APGSKEPCVLETEISLRNLESVNARVVRLEESVVSLLELLKEVLSRPGLSKDSLRHRD
                     "
     gene            3965..4834
                     /locus_tag="VFI43_03350"
     CDS             3965..4834
                     /locus_tag="VFI43_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HET7061195.1"
                     /translation="MKQISQISQISLSNAMTIDVEDYFQVSAFASHIPREAWPSISCR
                     VESNIDRILALLSEENIKGTFFTLGWIAERHPEMVKRIVSGGHELASHGWGHHRVTDQ
                     KPDEFREDVVRSKGILEDIGGQVVTGYRAPSFSIGGSNQWALDVLSEAGYLYSSSIYP
                     IQHDHYGMPDAPRFAYYPRGETGLLELPITTVRFYGRNIPAGGGGYFRLWPYPVSRWF
                     LRRLNRLEGRSAIFYFHPWELDHQQPRQKGISMKTRFRHYYNLHRMEERIKALSRDFK
                     WDRMDRVFLAGRA"
     gene            4831..5922
                     /locus_tag="VFI43_03355"
     CDS             4831..5922
                     /locus_tag="VFI43_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HET7061196.1"
                     /translation="MSCVIKRVEPDCGNHRLTEALRPSVRLLDSREWKRWDNFVNACP
                     QATFFHRAGWQTVLEQALGHKTWFLYAESEGEIQGVLPLAEINSRLFGHSLSSLPFCV
                     YGGIAAISERARQALDSEAQLLAARLQADYLEYRNLMPSHPQWPTKDLYVTFRKSIEA
                     EAEANMQGIPRKQRAMVRKGIKAGLQGHIDDGIDRFFSAYSASVHRLGTPVFSKKYFR
                     LLKQTFGQDCELLVITSEEGKTISAVMSFYFRDEVLPYYGGGTHEARELAGNDFMYWE
                     LMRRACERGCKVFDFGRSKRNTGSFDFKKNWGFEPQALYYEYQLHQARSIPDHNPLNP
                     KYQRFIKAWQKLPLPVANLIGPHIVKNLG"
     gene            5927..7186
                     /locus_tag="VFI43_03360"
     CDS             5927..7186
                     /locus_tag="VFI43_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET7061197.1"
                     /translation="MEELLYLAHRIPYPPNKGDKIRSYHLLKHLSQRYKVHLGAFIDD
                     ESDRKYHDRVKSLCGETCLVNLNPKVARVRSLQGFFSGQPLTLPYYRDAGLQQWVNDI
                     LKTRTIGTVLVFSSAMAQYVGHLTTTPRIIDFVDVDSDKWLQYSSAKKWPLSWIYRRE
                     AKLLLKYEQWIAGNFSSSTFVSKAEADLFRQLAPNAVKTISYFNNGVDSDYFSPEHDY
                     PNPYGEDKPVLVFTGAMDYWANVDAVTWFSRAIFPAVRLRVPEVEFFIVGARPTTEVR
                     ALAELPGITVTGSVPDVRPYLAWASLAVAPLRIARGIQNKVLEAMAMGKIVVASAQAM
                     EGIDAIAGQELVVPQNEKTFADEILMLLCDKKRPAMGTAARRCALERYSWKKNLERID
                     ALIVQARKEGEIQVPVRIGDSLREMRV"
     gene            7183..8784
                     /gene="xrtA"
                     /locus_tag="VFI43_03365"
     CDS             7183..8784
                     /gene="xrtA"
                     /locus_tag="VFI43_03365"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HET7061198.1"
                     /translation="MSAQTTRELPSPSTITDQQNFKLSVTIASVTVVVILLSYFQTAW
                     SMVSIWGRSDTFAHGFLIFPFSAYLIWSQRSQLASVNIQPNLPALLVLAGVGLGWLLA
                     TLASAQVVAQFFLIAMIPASVWVILGTNMVRALAFPLGYLLLAVPFGEALIPPLINFT
                     ADFTVRALQLTGIPVYREGSFFTIPSGNWSVVEACSGLRYLIASFTLGTLYAYLTYRS
                     LPRRLIFITLSVIVPILANGIRAYLIVMTGHLSEMRLAVGIDHLIYGWVFFGVVMLLL
                     FWIGTFWREDQPSDNATVRKYAIAPNPLRSEAPLKKILFATGATMAGALVWPAYAAFL
                     ENGFATHERPATLDIAGIPQKWEVNSGEVSGWRPYYVGPTAQLAQYYRSSDRPERTIG
                     LVVTYYRNQQQGSELINSQNLLTPEDGGSDESRWRDVRHDVRALNLGSGVQEINQNLV
                     QSSSLKLLVWRTYWLGDEITASPYVAKLILAKNKLLGRPDDGAEIIFVTAYDESPDEA
                     IPALERFGKEMIPSIISGLRNAAGS"
     gene            8808..9974
                     /locus_tag="VFI43_03370"
     CDS             8808..9974
                     /locus_tag="VFI43_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET7061199.1"
                     /translation="MKSNGQPPLIAHVIYHLGVGGLENGLINLINHIPPERYRHAIIC
                     LKGYTDFRGRIKRNDVEVLALNKREGNDLGLYVNLFKTLKRLKPDIVHTRNLGTMEGQ
                     VVAVLAGARARVHGEHGRDMSDLYGRNRKYNLLRRAIRPFVNHFIAVSKDLESWLLSS
                     VGAQSRRVSQIYNGVDSIRFYPGKAKCLAASPIARLPEGFITGDSFVVGSVGRMAEVK
                     NFPSLVQAFLMLLKQNPSVRPRLRLLVIGDGNSRDTCIRMLREGGAEDLCWLPGERGD
                     IPELMRMMDLFVLPSLAEGISNTILEAMSSGLPVVATRVGGNVELVKEGISGLLVPAN
                     SPGDIASAISSYFFDPEMAVRHGAAARKQIETAFSMNAMTEGYLHVYDKAMGNL"
     gene            10264..12198
                     /locus_tag="VFI43_03375"
     CDS             10264..12198
                     /locus_tag="VFI43_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HET7061200.1"
                     /translation="MCGIVGLFDTRGKREFDRRLLAHLNQTQVHRGPDEGEVYMEPGV
                     GLGHRRLSIMDVSSGHQPLFNEDGSVAVIFNGEIYNFQELARELISAGHIFRTHCDTE
                     VIVHAWEEWGEQCVDRFRGMFAFGLWDRNRQTLFLARDRLGIKPLYYGLLKDGTFIFA
                     SELKAVLAHPDFSRELDFHAVEDYFAYGYVPEPKTIFREAKKLSPGHTLKLQHGQSMP
                     RLHQYWDVPFGPADSISLEEAQEELAERLRESVRIHLMTEVPLGAFLSGGVDSSAIVS
                     MMANLMDDPVNTCSISFGDPAFNESRYAQAVADQYHTRHDVEQVDHDDFELIDKLALI
                     YDEPFADSSSMPTYRVCELARKRVTVALSGDGGDENLAGYRRYRWHLYEERLRSSLPA
                     QFRRPTFGLLGRVYPKADWAPRFLRAKSTFEALARDSVEGYFHGVSIVKDKMRRSMFS
                     PLFNKSLEDYRAVEVLRAHAGKCPARDPLSLVQYLDMKTYLVGDILTKVDRASMAHSL
                     EVRVPLLDHKLVEWISRLPASFKLQGSQGKYVFKKSLEPYLSNDILYRDKMGFAVPLA
                     SWFRGPLRGQLNNALLGSVLAETGFFNTAFIKEMLDQHQSGRRDHSAPLWTLLMFESF
                     LRNVLDVERSGTLKHNLKVA"
     gene            12358..13320
                     /locus_tag="VFI43_03380"
     CDS             12358..13320
                     /locus_tag="VFI43_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET7061201.1"
                     /translation="MLDVFFTVDVEVWCDGWEGIDSKFPDAFRRYIYGPTARGDYGLP
                     YQLRQLREYDLTGVFFVEPLFSTRFGSDPLAEIIGLIHEQGHEIQLHLHTEWVDESHR
                     PLLDNINGKKQFLRYFSLEEQIILIRAGADLVRQAGCERLDAFRAGGFGFNRDTLRAL
                     AHNRIPFDSSYNASIYGLDSGVSPEIPLVEPMECHGVHEYPMTVFRNGTSSLRHAQIT
                     ACSYREMEGLLWQALESRRKAFVILSHNFELLNEGMNRPDDIVVSRFRKLCSFFDRHR
                     DCFRLRGFRDLSPAAVLSQPLPLASPIWKTGLRMFEQGLRRGFR"
     gene            13317..14258
                     /locus_tag="VFI43_03385"
     CDS             13317..14258
                     /locus_tag="VFI43_03385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7061202.1"
                     /translation="MSPVWEYRQVPIKLQLGDKTLLEPKLWLQIREAVLGDENPPVAM
                     LTPPGDALLPESQGFLVRSLPISGVQPLLSRQNGYLCYVSSQYPRYYIDMRQSFEEYK
                     GKFSSKTRSTLNRKIRKFSEHCGGEIQWKVYRTVGEMRDFFKLARVVSEKTYQEKLLD
                     AGLPGSEEFLGEMEYLAAQDRVRGFILFHGGKPVSYLYCPIDKNVLIYAYLGYDPAYM
                     NFSVGTVLQWLALERLFEENSFRFFDFTEGQSDHKKLFATGSVHCANVFFLRTSPRNL
                     FLSYTQMSVNRFSKAAGDLLERLGVKTAVRRLMRFGA"
     gene            complement(14414..14605)
                     /locus_tag="VFI43_03390"
     CDS             complement(14414..14605)
                     /locus_tag="VFI43_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7061203.1"
                     /translation="MIMNKDQVKGTAKDIAGKVEEEAGRAVGSKEHEAKGLGKQVSGK
                     AQKKYGDAKEAVKDAADDL"
BASE COUNT         3482 a         3909 c         4127 g         3673 t
ORIGIN      
        1 tttgcgatca gccagatccg gccgaccttc catagccagg tcagcctaag ggaagcaaca
       61 ggccgcccta ttttgggctc tattcccatg atctggacca gcgcggaaaa aataaagcgc
      121 aggaagcgtc tatacgcctt cgggatatcc ttgctatccc tgctgggttt atacggagcg
      181 ctgatggtaa agatggccta accgtgactc acgggacagg cagcgcgtgt tacgccggga
      241 attgttgccg ggaattcgtg aggcgagatt atttcaagta gagtgcttat atccgtcatg
      301 cgcgcgtttt aacttctcat ttctaccaat ggtttcgttg atagtttatt tccatcatat
      361 gagtcaatcc ccgctcgtgg gcatactgcc cgttggcttc ccatgggaga acgtgtgagc
      421 attatagaga gagcggctgg tcagctcggg gcggagaaac aagcaggccc ggaaaggata
      481 tcgcagacgg gacatctcaa tccggttcag aaatcttccc actcagggga ggtggaaacc
      541 acggtggaac aagcgattca tgcagacagt gatcaggcaa caagacctgc aggatcgcgg
      601 acgggcatga gccagggtcc cgcgcaaacg accaggagaa ttattctcaa tcaggaaaaa
      661 atgcgtcagt acgggatcgt aaccccggat cagggaagga cccagatagc tgagcagttc
      721 cgggtgatca agcgcccgct gttgaccaac gcatttaaca gggggccggg gatgatcaag
      781 aacggcaacc tgatcatggt gacgagtgcg cttgcggggg aggggaaaag cttttgcaca
      841 gtgaacctcg ccatgagcat cgcaatggaa atggatcgca cggtgctgct cgtggacgcg
      901 gacgtggccc gtcccacatt gccgagaatt ctcggacttg gaacggaaag aggactgctc
      961 gatattcttc ttgatgaaaa actcgagttg gccgacgttc tgatcaagac tgatatcgag
     1021 aagctcactc tcctgactgc aggaaggcgg cattcacatt cgaccgaatt gttggcaagc
     1081 caaagcatga atgtcttgct gaaggaactt gcggagcgat acgcagatcg cgtggtcatc
     1141 tttgattctc cgcctttgct gctgacgagc gaggcgcggg tgttggcaac ccagatgggg
     1201 caaattgtcc tcgtagtgga ggctgaaaga acatctcaac aggcggtcaa ggagactctc
     1261 cggcaaatcg agtcatgcga cgtggtaaat ctcatctata acaaggccag atcattccca
     1321 ggcggcgaat actacggcta ttattaccat gaaagcgcct gaccccaggt gccgcaaatc
     1381 ctcgtttcct gttactttcc ccgcggtagt agcgatattc ctgctttcct cgaacgctga
     1441 tgcgtcggag tggaagattc ggccgcgcat gaatctcata gaaacttatt cggataactt
     1501 gaggctgcga tcagggtctg ggtcggacga tttcatcact caaatcaacc cgggcattct
     1561 ggtcaatggc gtggcgagcc gctataccgt ggatgtgaat tacctcatga acaatcttat
     1621 ctatgcgcaa aacggcaact tcaaccggat ccagaaccag cttaatgcca atggcactgg
     1681 cgaactgatc aaggatttgt tcttcgtcga tggaagagcc tttatcacgc agcagaacgt
     1741 ttctctgctt ggaccccagg ctattaataa cgtcaacgtt acggggaaca ggactaacgt
     1801 aagcacatac gttctcagcc cctatttgcg ccatcggttc cagaatttcg ccacgaccga
     1861 attgcgttac acccgtaact gggtcaactc gagcacgaac gggcttgcca gcagcaccgg
     1921 agacggcgtg ctgttcaacc tgaacagcgg aacggcattc aagacgtttg gttggggagt
     1981 gaactacagt aaccagatga tccatttcga ttccgggcgg gacatcgagc tggagcgttc
     2041 aacaggaacc ttgcgttaca tgttaacccc ccagttcggg ctgaacgcga ccggtggcta
     2101 tgaaaggaac agttttgttt ccatcagggg aagtccctct gcgcccctct ggaccgttgg
     2161 ttttgtctgg gagccgactc aacggacaaa tatctccgtg agtggcggtc agaggttttt
     2221 cggaaatacg tataacgcgc tagcgagcca tcggacccgc ctcatggtgt ggagctttgg
     2281 ttataaccag aacattacca ccttcaatca gcagagtgct tttggcaccg gaattccggg
     2341 gagccttgga ggttccctga atcagctgct gactgctcaa aatcccaatc tcagccccga
     2401 tgtcatccag caaaacactg ctgctcttac gggtatcggg ctctcaggtt ctttttttaa
     2461 tcccatgaat ttcctcacca accgcttgtt cctccagaag accctgcaag cttctgtcgt
     2521 catgaatggt tccagaaata cgatcacggc cagtcttttc aatatgaccc ggcaggccta
     2581 ttccgacccc ttggccgata ttaacctcgt tggggcgcaa aaccttgccc ttctcaatca
     2641 tacgaggcaa agcggggtca atgctctttg gaactatcgg ttatctgaac gaacgagcgc
     2701 taatctcaat tttgcctata cgaggttcaa gtttcttggc gcgggaaggg aggacgatct
     2761 catgcttgtc atgctgggct tgacccggca attcagatca attcagccta atctaagcgg
     2821 cacgcttcaa gtcaggcatc agcaacgcga ctcgaatcaa ccaggaagcg attatcgtga
     2881 gcaagcggtt atcggatcgc taaacatgag tttttagcgc aatcgaatgt atccatcgtt
     2941 ctatggcttc agggtcaagc cattcaagct gaaaccggat ccggattttt actttggcag
     3001 caaaggccat aagcgggcta tggcttatct cgaatacgga ttggctcaag gagaagggtt
     3061 cattgtaatt actggcgatg tgggcgccgg aaagacgact cttgtgcgat atctgttccg
     3121 taagctcgat tcaaaggaaa ttgttgctgc ccatatcgcc aacactgatc ttgattccgg
     3181 cgacacactc aggatggtcg cgtcagcgtt tggtctgcct tttgaaaacc tgagcaaagc
     3241 ctctttactg atcagcattg aacaattcct gcaagcatgc gatcagcagg ggaagcgcgc
     3301 gcttctggtg gtcgacgaag cgcaaaacct ttctccccgc acggtggagg aattaagaat
     3361 gctttccaat tttcagacgg aagaaaaacc tttattgcaa acgttcctgc tcggccagcc
     3421 ggaattccgg aaaacgctgc taggggcgga tatgcagcaa ctgcggcaaa gggtgactgc
     3481 aacctatcac cttggaccca tggacttgcc ggaaacacgg gcgtatatcg aacacaggct
     3541 ccgaacagcg ggttggcaag gagaccctgc attcaaagac ggcgcttttg aagcgattta
     3601 cggaagttcg ggcggcatac ctcgcaagat aaacgcgctg tgtgaccgcc tgctgctcat
     3661 gggttatctc gaaaagctgc acgagttcgg cgaggaggag gtcaacgagg tcatcaacga
     3721 tattcagcag gagttcgagg ttgccattac accggcgcct ggctcaaaag agccctgcgt
     3781 gctggaaacg gaaatctcgc tgcggaatct cgagagcgtc aacgccaggg tggtgaggct
     3841 ggaggaatcg gttgtctcac tactcgagct tctcaaggaa gtgctttccc gtccaggctt
     3901 gagcaaggat tccctcaggc accgggacta gcaagcgcgt ttactttttc aacaagctct
     3961 atccatgaag cagatatccc agatatccca gatatccctt agcaacgcaa tgaccataga
     4021 cgtggaagat tacttccagg tttcggcatt tgcctctcat atcccccggg aggcttggcc
     4081 ctcaatatcc tgccgggtgg agtcgaatat tgaccgcatc ctcgcgctgt tgagtgagga
     4141 aaatataaaa gggacttttt ttaccttggg gtggattgct gaacgccatc cggaaatggt
     4201 gaagcgaatc gtgtcggggg ggcacgagct tgccagtcac ggatgggggc accatcgagt
     4261 gacggatcaa aagcctgatg agttccgaga agatgttgtc cggagcaaag gcattctgga
     4321 agatatcggc gggcaagtcg tgactggtta ccgtgccccc agtttttcca tcggcggcag
     4381 caatcaatgg gccctggatg tgctttccga agccggctac ctctacagtt cgagtattta
     4441 tccgatccag catgatcatt acgggatgcc ggatgcgccg cgtttcgcct attacccccg
     4501 aggtgagacg ggtttgcttg aacttcccat cactaccgtt cgcttttatg ggcggaatat
     4561 tcccgcaggg ggcggcggct atttccgcct ttggccctat ccagtttcgc ggtggtttct
     4621 acgccggctg aatcggctgg agggccgctc cgcaatcttt tatttccatc catgggagct
     4681 tgaccatcaa caaccgcgtc agaagggcat cagtatgaaa acgcgctttc gccattatta
     4741 caatctccac cgcatggagg agagaattaa agcactgagc cgggatttca agtgggaccg
     4801 catggatcgg gtttttctgg ctggacgcgc atgagttgcg tcataaagag agtcgaaccg
     4861 gattgcggga atcacaggtt gaccgaggcg ctgagaccat ccgtgcgttt gctcgattcc
     4921 cgagagtgga aaagatggga taactttgta aacgcatgcc cgcaggcaac gtttttccat
     4981 cgggcgggtt ggcagacggt tcttgagcag gcgcttggac acaaaacctg gtttctctac
     5041 gcggaatcgg agggggaaat acagggcgtg ctgcccttgg cagagatcaa tagccggctt
     5101 ttcggccatt ccctgagttc gcttccattt tgtgtctacg gagggattgc tgccatctct
     5161 gagcgggcgc gacaagccct ggacagcgaa gcccagctgc tggccgcacg tttgcaagcc
     5221 gattatctcg agtaccgcaa tttgatgccg tcgcacccgc agtggccgac aaaggatctt
     5281 tacgtcacgt ttcgcaagag tatcgaagca gaagcggaag ccaatatgca ggggattccc
     5341 cgcaagcagc gggccatggt gcgcaaggga atcaaagccg ggctacaggg ccatatcgat
     5401 gacggtatcg atcgcttttt ttctgcgtat tcagccagtg tgcatcggct tgggactccc
     5461 gttttttcaa aaaaatattt tcgcctgctc aagcagactt ttggccagga ttgtgaactc
     5521 ctggttatca ccagcgaaga aggcaagacg ataagtgcgg tgatgagttt ttattttcgc
     5581 gatgaggtct tgccttatta cggcgggggc actcatgaag cacgtgaact ggcaggcaac
     5641 gatttcatgt actgggagct gatgcggcgt gcctgtgaaa gaggttgcaa agtctttgac
     5701 ttcggccgaa gcaaacggaa tacgggttcg ttcgacttca agaaaaactg gggtttcgag
     5761 ccgcaagccc tttattacga gtaccagctt catcaagcgc gttcgattcc cgaccacaac
     5821 ccgctcaatc caaaatatca gcgatttatc aaggcgtggc aaaagcttcc tttgccggtc
     5881 gccaatctga ttggcccaca catcgtaaaa aacctcggct aagccaatgg aggagcttct
     5941 ttatctcgcg catcggattc cttatccgcc gaacaaggga gacaagatcc gctcatatca
     6001 tttgctcaag cacttgagcc agcgttacaa ggttcatctt ggtgctttta ttgatgatga
     6061 gagtgatcgc aaatatcacg accgcgtcaa aagtctttgt ggcgaaacct gtctcgtgaa
     6121 tctcaacccg aaggtagccc gagtgcgcag ccttcaaggt tttttttcgg gccagcctct
     6181 tacacttcct tattaccgcg acgcgggctt gcagcagtgg gtaaacgata ttttgaaaac
     6241 cagaacgatc ggaacggttc tggttttttc gtcagccatg gcccagtacg tcgggcactt
     6301 gaccactacg cctcgaataa tcgactttgt cgatgttgat tcggataaat ggctgcaata
     6361 ttcatccgca aaaaagtggc ccttgagctg gatttaccgc cgggaggcca aactgcttct
     6421 gaagtatgaa cagtggattg cggggaactt cagcagctcg acctttgttt caaaggcaga
     6481 agcggatctg ttcaggcaac tcgcaccgaa cgctgtaaaa acgattagct atttcaataa
     6541 tggcgttgac tcagactatt tctcacccga acacgattac ccgaatccct atggggagga
     6601 caagcctgtt ctggtattta ccggcgcaat ggactattgg gcgaatgtcg acgccgtgac
     6661 ctggttttca cgcgccatat ttccggccgt acgcctccgc gtcccggaag ttgagttttt
     6721 tattgttggc gctcggccaa ccaccgaggt ccgggctctc gccgagcttc ccggcatcac
     6781 ggtaacaggt tccgttcctg acgttcgacc ctacctcgcc tgggcgtcgc tggctgtcgc
     6841 tcccttgcgg attgcccggg gcattcagaa caaggtactg gaagcaatgg caatgggaaa
     6901 gattgtggtt gcctctgctc aagcgatgga gggaattgac gcaatcgcag ggcaggaatt
     6961 ggtagtgcct cagaacgaaa agacctttgc cgacgagatt cttatgcttc tgtgcgataa
     7021 aaaacgcccg gccatgggta cggcggcaag gcgttgcgct cttgaacgtt acagctggaa
     7081 aaaaaatctt gagaggatcg acgcgctcat tgtccaggcg cggaaagagg gagaaatcca
     7141 ggtccccgtc cgcatcggcg acagcctgcg ggagatgaga gtgtgagcgc acaaacaaca
     7201 agagagctac caagtccctc cactattacc gaccagcaga atttcaaact atcggtaacg
     7261 attgcttcgg ttaccgtcgt cgtcattctg ctcagctatt ttcagacggc ctggtccatg
     7321 gtttcgatct gggggcgatc agacacattt gcgcatggct ttctgatctt ccccttcagt
     7381 gcctatctga tatggagcca gcgcagccag cttgcctcgg tcaacattca gcccaacctt
     7441 cctgcactcc tggtactcgc aggagttggc ctcggctggc tcttggcaac gctggcgagc
     7501 gcccaggtcg ttgcgcagtt ttttctcatt gccatgattc cggcctcggt ttgggtgatt
     7561 ttagggacca acatggtgcg cgcccttgcc ttcccccttg gatatctgct gctcgccgta
     7621 ccatttggcg aggcgctcat ccctccatta ataaacttta ctgccgactt cactgtgaga
     7681 gcgctgcaac taaccggcat tcccgtctac agggagggaa gcttttttac tattcccagc
     7741 gggaactggt ccgtggtcga agcctgtagc gggctgcgct acctcattgc ctcttttacc
     7801 ctgggcactc tttatgccta tttgacctac cgcagcctcc cccgtcgtct catatttatc
     7861 accttgtcgg ttatcgttcc aatcctggcc aacggcattc gagcctacct gatcgtaatg
     7921 accgggcatt tgagtgaaat gcgtctggcc gtgggaattg accatcttat ctacggatgg
     7981 gtcttttttg gcgtggtgat gcttttatta ttctggattg gcaccttctg gcgcgaggat
     8041 caaccgagcg ataacgcgac tgtgcgaaag tacgccattg cccccaatcc gcttcggagc
     8101 gaggctccgc tcaaaaaaat cctgtttgcg actggggcga ccatggctgg cgcccttgta
     8161 tggccggcct acgctgcttt tctcgaaaac gggttcgcca cccatgaacg tccggccacg
     8221 ttggatattg ccggcattcc ccaaaaatgg gaagtgaact ccggagaagt ctccggctgg
     8281 cgcccctact atgttggtcc tacggctcag cttgcgcaat attaccggag cagcgatcgg
     8341 cccgagcgaa ccattggcct cgtcgtgact tattaccgta atcagcagca gggcagcgag
     8401 ctgatcaact cgcagaatct gcttacgccg gaggatggcg ggtctgatga atcgcgatgg
     8461 cgagatgtca ggcatgatgt gcgagctctc aacttaggct cgggcgtgca ggaaatcaac
     8521 cagaacctcg tacaatcttc ttcgctgaag ctgctggtat ggagaacata ttggctgggc
     8581 gacgaaatca ctgccagtcc gtatgtcgcc aaacttatcc ttgcaaaaaa caagctgctg
     8641 gggcggcctg atgacggagc agaaattatt tttgtaacgg cttacgatga gtcgcccgat
     8701 gaagccatac ccgcgcttga acgcttcggc aaggagatga tccctagcat catatccggt
     8761 ctcaggaatg cggcgggcag ttagcggtac gcggtctggt taagcgcatg aaatcgaatg
     8821 gtcaacctcc tctcatcgcc cacgtaatct accatttagg ggttggaggg ttggaaaacg
     8881 ggctgatcaa tctgatcaat cacattcccc ctgagcgtta ccgtcacgct atcatttgcc
     8941 tcaagggata tacggatttc aggggccgga tcaagcgaaa cgacgtggaa gttttggcgc
     9001 tgaacaagcg agagggaaac gacctcggtc tttacgtgaa ccttttcaaa acactgaaga
     9061 gattgaagcc cgatatcgtg catacacgga atctcgggac gatggagggg caggtcgttg
     9121 ccgtgctggc tggcgcacga gcaagggtgc atggagaaca cggtcgtgac atgtccgatt
     9181 tgtacggcag gaaccgcaaa tataatctgc tgcgacgagc gatccggcct tttgtgaacc
     9241 attttattgc cgttagcaag gatttggaga gctggcttct cagttcggta ggcgcgcaat
     9301 ctcgacgcgt cagccagata tacaacggcg tggatagcat tcgtttctat cccgggaagg
     9361 cgaaatgtct tgccgcttcg cctattgcaa ggttgccgga aggattcatt acgggggatt
     9421 catttgtagt tggcagcgtg ggaaggatgg ccgaggtgaa gaattttccc agcctcgtac
     9481 aagccttctt gatgctgctg aagcaaaatc cttcagtccg tccgcgcctc cgtctgcttg
     9541 taatcggaga tggaaattcg cgtgatactt gtatccggat gctgcgggaa ggtggagcag
     9601 aagatctatg ctggctgccg ggagagcgtg gggacatacc ggagttgatg aggatgatgg
     9661 acctgttcgt gcttccctca ctcgcggaag gtatttccaa taccattctt gaagcaatgt
     9721 cgagtgggct gccggttgtc gcaacccggg tgggtggaaa cgtggagttg gttaaagaag
     9781 gaatcagcgg attgcttgtt ccagcaaatt cgcccggaga tatcgcttcg gcgatctcaa
     9841 gctatttctt tgatcctgaa atggctgtcc gccacggggc ggcggcgcga aaacagatcg
     9901 agacagcttt cagcatgaac gccatgacgg aaggctatct ccatgtttat gacaaagcca
     9961 tggggaatct ctgaacaagt catcatgagc gcgacaacgg ttttgcggga tgggatgcgc
    10021 gaaaggcaac gaagcgaatg gtcaatcccc ttcggcagga gcctgacaaa gcagatcccc
    10081 cgcaaagcct tgtcccacag gggttgcggt aaaatcgggc gctcacggcg ttgcactcct
    10141 tggcatcgta gacccggcta cgacctgcgt cgcgcgcctt gtgatcatcg cgatttgacc
    10201 agcagcgcgc ttcacgagga cttgttcaga gattcctcaa taagttgaga tagaggaaaa
    10261 agtatgtgtg gaatcgtcgg cttatttgat acgaggggca aaagggagtt cgatcgccgg
    10321 ctgcttgcgc acttgaatca gacccaggtc caccgaggtc cggatgaagg cgaagtgtac
    10381 atggaacctg gcgttggtct gggacatcgc cgcctttcaa tcatggatgt atccagtggt
    10441 caccagccac tgttcaacga ggacggcagt gtcgccgtga tcttcaatgg agaaatttac
    10501 aattttcagg agctggcaag ggaattgatt tccgcgggtc atatattccg cacccactgc
    10561 gataccgaag ttattgtcca cgcttgggaa gagtggggag agcaatgcgt cgatcgcttc
    10621 cgaggcatgt ttgcattcgg actatgggat cggaatcgcc aaacactgtt ccttgcccgg
    10681 gaccggttag gcataaagcc tctctactat ggcttactga aagacgggac attcattttc
    10741 gcctcggagc tcaaagcggt gcttgcccac ccggattttt cgcgagagct cgattttcac
    10801 gcggttgagg attattttgc ctacggctac gtaccggagc ccaagaccat attccgggag
    10861 gctaaaaaat tatctccggg tcatacgctg aaacttcagc acgggcaaag catgccgagg
    10921 ctgcatcaat actgggatgt gccctttggg ccggccgatt cgataagcct ggaggaggcg
    10981 caggaagagc tggctgaacg gctgcgggaa tcggtccgaa ttcacttgat gactgaggtg
    11041 ccgctcggtg ctttcctctc gggcggcgtc gattcgagcg cgatcgtttc gatgatggcc
    11101 aaccttatgg atgatcctgt caacacttgc tccatctctt tcggagaccc agctttcaac
    11161 gaatctcgct acgcccaggc agtggcggat caataccaca cccgtcatga cgtagagcag
    11221 gtggatcatg acgattttga gctgatagac aaactggcgc ttatttatga cgaacccttt
    11281 gccgacagct cctcgatgcc tacataccgg gtatgcgaat tggcaagaaa acgtgtaaca
    11341 gttgcattat cgggtgacgg aggtgacgaa aatcttgccg gctaccgccg ctaccgatgg
    11401 cacctttatg aagaacgatt gagatcttcg cttccggccc aatttcgcag gccgacattc
    11461 ggtttgctgg gaagggttta cccgaaagcg gactgggctc ccaggttttt gcgggcgaag
    11521 tcgacctttg aggctctggc gcgtgattca gtcgaaggat acttccacgg tgtatcgatt
    11581 gttaaggaca aaatgcggcg aagcatgttc agcccattgt tcaataaaag cctcgaagac
    11641 taccgggcgg ttgaagtatt gcgtgcgcat gcggggaaat gtcctgcccg agacccgctc
    11701 tcactggtgc aatacctgga catgaagacc tatctcgtgg gagatattct taccaaggtg
    11761 gaccgcgcaa gcatggcgca ctcgctggag gtgcgcgtgc cgcttctgga ccacaagctt
    11821 gtcgagtgga tttcccgctt gcccgcatca ttcaaattgc aaggcagcca gggaaagtat
    11881 gtattcaaga aatccctgga accatacctt tcaaacgaca tcctgtaccg ggacaaaatg
    11941 gggtttgccg tgcctctggc aagctggttc cgtggtccgt tgcgggggca actgaacaat
    12001 gcgctgctgg ggtctgtcct ggcggagacc gggtttttta acacggcttt tatcaaggag
    12061 atgctcgacc agcaccaatc cggacggcgt gaccacagtg ctccgctttg gacactgctg
    12121 atgttcgagt catttttacg caatgtcctt gacgtggagc gttcaggaac actcaagcat
    12181 aacttgaagg ttgcgtagtt tcttccacga gcccgctggc catctactca agctatggtg
    12241 aggacccgcc tcaggaagga agccgctgcg aggggcaacc tgttctctca ccgaagctag
    12301 gtcttcctct gcgccagttc accgatcctt tccagatatg ctaaatgctc attaaaaatg
    12361 ctcgatgtat tttttaccgt tgacgtggag gtatggtgcg atgggtggga ggggattgac
    12421 tccaaatttc ccgacgcgtt tcgacggtac atctatggtc ctaccgcgcg gggtgactat
    12481 ggcttgccct accagttgcg ccaattgcga gaatatgacc tgacaggagt atttttcgtc
    12541 gaacccctct tctccacccg gttcggatcc gacccgctgg ccgaaatcat aggtcttatt
    12601 catgagcagg ggcatgaaat acagcttcac cttcataccg agtgggtgga cgaatcacat
    12661 cgacctctgc tcgacaatat caatggcaag aagcagttcc tgcgatactt ttcgctggaa
    12721 gagcaaatca tcctgatccg ggccggagcg gacctggtca ggcaggccgg gtgcgaacgt
    12781 ttggatgcat tccgggccgg gggcttcggt ttcaacaggg acactcttcg cgcacttgcc
    12841 cataatcgca ttcccttcga cagcagttac aatgcaagca tatatggcct ggatagcgga
    12901 gtcagccccg agattccgct cgtggagccc atggaatgcc atggcgttca cgagtatccg
    12961 atgaccgttt ttcgtaacgg cacttcctcg ctgaggcatg cccagataac cgcatgctcg
    13021 taccgggaaa tggaaggcct gctatggcaa gcgctggaat cgaggcgaaa agcttttgtt
    13081 atcctctctc ataattttga actgctcaat gagggcatga atcgccccga cgatattgta
    13141 gtcagccgtt ttcggaagct ctgctctttt tttgatcgtc accgcgattg cttccgcctg
    13201 cgaggctttc gtgatctatc ccccgctgcc gtcctttcac agccccttcc tcttgcttcg
    13261 ccaatctgga aaacgggatt gaggatgttt gagcaggggt tgcgccgggg gtttcgttga
    13321 gtccggtatg ggagtatcgc caggtaccca taaagctaca acttggcgac aagacactgc
    13381 ttgaaccgaa gttgtggttg caaataaggg aagcggtgct tggcgatgaa aatccgcctg
    13441 tagccatgct tacacctcct ggggacgctc tcctgcctga gagccagggt ttcctggtac
    13501 ggtccttgcc catctcgggc gttcagccct tgctgagcag acagaacggt tacctttgtt
    13561 atgtctcgtc acagtacccc cgctattata ttgacatgcg gcagtccttc gaggaataca
    13621 aaggcaagtt ttcatccaag acgcgcagca ctctcaaccg caaaatcagg aaattttcag
    13681 agcattgcgg gggcgaaatc cagtggaagg tttacaggac ggttggggag atgcgtgact
    13741 ttttcaagct ggcacgtgtc gtttccgaga aaacctatca ggaaaaattg cttgacgcag
    13801 gactcccggg ttcagaagag tttctcggcg agatggaata cttggctgca caagaccgtg
    13861 tgcgcggttt cattcttttt catggcggca aaccggtctc ctacctctac tgccccattg
    13921 acaagaacgt cctgatttat gcttacctcg ggtatgaccc ggcatacatg aatttttcgg
    13981 taggcaccgt tttacagtgg ctggctctcg agcgcctgtt cgaggaaaat tcgtttcgtt
    14041 tttttgattt tactgaagga cagtcggatc ataaaaagct ttttgctact ggtagtgtcc
    14101 attgtgcgaa cgtctttttt ttgcgaacca gtcctcgtaa tcttttcttg tcgtataccc
    14161 agatgtcggt caaccggttc agcaaggcag ccggagattt gcttgagcgg ttgggtgtaa
    14221 aaacggcggt tagaagactg atgcgattcg gggcatgatc gccccgcaat tttgatagac
    14281 tcactcggcg atgcacctcg tgtcagaacc tgtagccaag catggtcttg tagggggttc
    14341 ataaacaaac ccgccaaagc gggaacaaat aaaaacccgc cggagcgggt ttagggaaag
    14401 tctcggactc ggcttaaaga tcgtctgcgg cgtcttttac ggcttccttg gcatctccgt
    14461 atttcttttg ggctttgccg gagacttgct tgccgagccc tttggcttcg tgctctttac
    14521 ttcctactgc ccttccggct tcttcctcaa cttttccagc gatgtctttc gcagtccctt
    14581 tcacttgatc tttattcata atcatctcca gttaaaaacg taagtttcgc tttgtcgttg
    14641 cagtcgctac tgctacgacg ttggaaccag attaacgtct tttggcgtct catactgttc
    14701 ggtatgccac atactgttct gtttgccact cgatgatagg gacggaacgc gcgaagacaa
    14761 caatacatct gcagggggac aaatgctcta ttgatcccac ttacgtaatc aattctgcag
    14821 aattatagcg agaagcattc tgttcactca tgccgcgctg attcctgatt cctgattcct
    14881 gattccgggg acgaagtggt gcagccgtcc gcatcataaa atgctcttgt tacatctatc
    14941 gccggtatct cgtttgttgg gagttcatct ccgacgtgag agccaggcgc gcgaccgcct
    15001 ctactgtacc aaagtccaat tgttccatcc gtcgaagttg ctagcatgtc aactactcaa
    15061 aacgtgcaac agcagaattg cggaatccat ggggaatgtc atcaagcgct tagctcaggc
    15121 tatattcagg gattactcct tctaccacat ctatgagtat gaatacgcag gtgaaacacc
    15181 agtaccaaat c
//
LOCUS       DASSHJ010000081         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1261694,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000081 DASSHJ010000000
VERSION     DASSHJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261694"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFI43_03395"
     CDS             <1..529
                     /locus_tag="VFI43_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7061204.1"
                     /translation="TQVCAACHNPDGNSVIPANPTLAGQHAEYITKQLENFKSQDGKP
                     AERPSAVMGAMAAPLSEEDMKNLGAYYAQQTPKPGGAKDKALAEQGEKIYRGGNLETS
                     VPACSACHSPNGVGIPPSYPRVAGQHSEYVLAQLRAFRTEERTRDPNNVMHMIASRMS
                     EKEMRAVSEFISGLR"
     gene            complement(551..1837)
                     /gene="hemL"
                     /locus_tag="VFI43_03400"
     CDS             complement(551..1837)
                     /gene="hemL"
                     /locus_tag="VFI43_03400"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET7061205.1"
                     /translation="MTSRNQQLFEQSQQYIPGGVNSPVRAFKSVGGTPIFFQRGKGSH
                     VWDVDGKAYIDYVGSWGPLILGHAHPEVVEAVRVAAGNGLTFGAPTEAELEIAELICR
                     LVPSIEQVRLVSSGTEAGMSAVRLARGYTRRNRIIKFEGCYHGHDDALLVKAGSGALT
                     FGHPSSAGVPEETAASTVVVDFNDLASVEQAFDRFGDEIAAVIVEPIAGNMNLVVPKP
                     GFLERLRELCSLHGTILIFDEVMTGFRVGLKCAQGLYGIKPDLTTLGKVIGGGMPMAA
                     FGGRRDIMQCLAPVGAVYQAGTLSGNPIAVAAGLATLRLVQAEGFYEKLAARTLQLTD
                     GLSAVARKQGVDFCAQAVGGMFGLYFSDELPSSYAEVMSCDRDGFNDFFHAMLQQGIY
                     FAPSAFEAGFVSAAHGETDVADTLAAAERAFEDGLD"
     gene            complement(1851..2495)
                     /gene="thiE"
                     /locus_tag="VFI43_03405"
     CDS             complement(1851..2495)
                     /gene="thiE"
                     /locus_tag="VFI43_03405"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7061206.1"
                     /translation="MRWPRIEGLYAITPDIADTAALIRMARQILVGGARLVQYRNKSA
                     TEALRLEQAHSLASLCREFRVPLIVNDHLDLAIEVSADGVHLGFDDTPIAHARRKLGT
                     RKIIGASCYNNLEQALEAEHQRADYVAFGAFFASTTKPHAVAATKELLRGAKALLRVP
                     VVAIGGITARNAMELINEGADALAVSHAVFAASDVQSEAQTFARLFERRDNFSH"
     gene            complement(2492..>3289)
                     /locus_tag="VFI43_03410"
     CDS             complement(2492..>3289)
                     /locus_tag="VFI43_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HET7061207.1"
                     /translation="DSSGGAGIQADILTLASMGCHPLSVITAITIQDTAGVDDVLAID
                     PEWVADQARAVLEDMPVHVFKMGMLGSVEVITAIAEVISDYPNIPLVLDPVLASGRGD
                     ELASDDMVMAMRELLLPQVTILTPNSLEARRLAQDDDDENDLADLALCADRLLQMGCE
                     YVLITGTHENTSQVVNNLYGAGGLVRTDSWRRLEGSFHGSGCTLASAIAAGLANGLPV
                     AEGVYEAQEYTWQSLKAGFRPGMGQYLPDRLFWAREEANGADAIRAK"
BASE COUNT          744 a          977 c          886 g          682 t
ORIGIN      
        1 gacccaggta tgtgccgctt gccacaaccc cgatggtaac agcgttattc cagccaatcc
       61 tacgctggcc gggcagcatg ccgagtatat caccaagcaa ctggagaatt tcaagtctca
      121 ggatggtaag cccgccgagc gcccgagtgc agtaatgggt gcgatggccg cccccctgtc
      181 ggaagaggac atgaaaaatc tgggtgcata ttacgcccag caaaccccga agccaggcgg
      241 ggcgaaagac aaggctctgg cagagcaggg tgaaaaaata taccgcggag gcaacctcga
      301 gacgagcgtt ccagcatgct ccgcttgcca ttcccccaat ggggtaggca ttccgcccag
      361 ctatccccgc gtcgcggggc aacattcaga atatgtactg gcacagttac gggcttttcg
      421 taccgaagaa cgcacgcgag atcccaacaa tgtcatgcac atgattgcat cacggatgag
      481 cgagaaggaa atgcgcgcgg tttccgagtt catttccggt ttgcgctgaa tctcgagatc
      541 accggccaga ttaatcaagg ccatcttcaa aagcccgttc ggctgcagcc agggtatcag
      601 caacgtcggt ttcgccatgt gctgcggaaa cgaatcccgc ttcgaatgct gacggtgcaa
      661 agtatatgcc ttgctgaagc attgcatgaa aaaaatcatt gaacccgtcc cggtcgcagc
      721 tcataacctc ggcataactt gacggcaatt cgtcgctgaa atagaggccg aacattccac
      781 ccacggcctg ggcacaaaaa tcgacgccct gtttcctcgc cacggcggaa agcccatcag
      841 taagctgaag ggttcgggct gcaagcttct catagaagcc ttctgcttgc acgagtctca
      901 acgttgccaa tcctgctgct accgcgatgg ggttacccga gagcgtgccg gcctggtaga
      961 cggccccaac aggagccagg cattgcatga tgtctcgtct gccgccaaag gcagccattg
     1021 gcatgcctcc gccaatgacc ttaccaagcg tcgtcagatc cggcttgatt ccgtaaagcc
     1081 cctgggcaca ctttaacccc acgcgaaagc cagtcatgac ttcgtcaaat atcaatatcg
     1141 tcccatgcag gctgcaaagc tcccgtagcc tttcaagaaa gccaggcttg ggtacaacga
     1201 gattcatatt ccccgcaatc ggttcgacaa tgaccgccgc gatctcgtca ccaaaccggt
     1261 cgaacgcctg ttctacactt gcaagatcgt taaaatcgac cacaacggta ctcgctgcgg
     1321 tctcttccgg aacaccggcg gagcttggat ggccgaatgt aagggcgccc gaaccggctt
     1381 tcacgagaag ggcatcgtca tggccgtggt agcaaccttc aaacttgatg atacggttcc
     1441 ttcgggtata cccgcgcgca aggcggactg cgctcatccc tgcttccgtc ccggaactca
     1501 ccaatcttac ctgctcgatg gaagggacaa gtcggcagat aagctcggct atttcaagtt
     1561 cggcctctgt tggagcgcca aaggtcaggc cgtttccggc tgccacccgt acggcctcga
     1621 ccacttccgg atgtgcgtgg cccaggatca gcggccccca ggaaccgacg tagtcaatat
     1681 aggctttgcc gtctacatcc cagacatgcg agcccttgcc gcgctggaaa aatattggcg
     1741 tgccccccac ggatttgaag gcacgaaccg gtgagttgac gccgcctgga atatattgtt
     1801 gagattgttc aaacagctgt tgattacgtg aggtcatggg gcagctcgtg ctaatgagaa
     1861 aaattatcgc gtcgctcgaa taaccgggca aaggtttgcg cttccgactg gacgtccgag
     1921 gccgcgaata ccgcgtggct tacagcgagc gcgtcggcgc cctcgtttat aagttccatt
     1981 gcgttcctcg ccgttatccc gccaatcgcc acaaccggaa cgcgcagcaa agcttttgcc
     2041 ccccgtagca attccttcgt tgccgctaca gcatgaggct tggtcgttga agcaaaaaaa
     2101 gcgccgaatg ccacataatc cgccctctgg tgctccgctt cgagtgcctg ctccagattg
     2161 ttataacagg aggcgccgat gattttcctg gttcccagct tacgccttgc atgagcgatc
     2221 ggagtatcat cgaaccctag atgaacgcca tctgcactga cctcgatggc aaggtcaaga
     2281 tgatcgttaa ctatcagagg gacgcgaaat tcccggcaga gagaggcaag cgaatgcgcc
     2341 tgctccagtc taagtgcttc agttgcactt ttgttgcgat attggaccag tcgtgctcct
     2401 ccgacgagta tctgccgcgc catccggatc aaggcagcgg tgtcggcgat atctggcgta
     2461 attgcgtaca agccttcaat tcgaggccat ctcatttggc cctgatcgcg tccgctccgt
     2521 ttgcctcttc gcgcgcccag aacaatctgt ccggcagata ttgacccatg ccgggacgaa
     2581 agccggcctt tagcgattgc caggtatatt cctgtgcttc atatacacct tcagcgacag
     2641 gcaggccatt ggcgagcccc gctgcaatcg cagacgcgag cgtgcagccg gagccgtgga
     2701 aggaaccttc cagccgccgc caggaatctg tgcgcacgag gcctcccgct ccgtacaggt
     2761 tgttgacgac ctgagatgtg ttttcgtggg ttccagtaat aagcacgtat tcgcagccca
     2821 tttgtagaag ccggtcggcg cacagcgcga ggtccgccag atcattttca tcgtcgtcat
     2881 cctgggcaag acggcgcgct tcaaggctgt taggggtgag aatggtcacc tgcgggagca
     2941 aaagttcgcg catcgccatc accatgtcat cactcgccag ctcgtcgccc cgaccggaag
     3001 caagcacggg atccaacacc aggggaatat tgggatagtc ggagatgact tcagcgatcg
     3061 ccgtaatgac ctcgacgctc cccagcattc ccatcttgaa tacgtgaaca ggcatgtctt
     3121 cgaggactgc ccgagcctga tcggctaccc attcagggtc gatcgccagc acatcgtcca
     3181 caccagcagt gtcctggatg gtaatagcgg taatcacgga taatgggtgg caacccatgc
     3241 tcgccagggt gagaatatcg gcctggattc ctgcgccgcc gctggagtc
//
LOCUS       DASSHJ010000082         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_899784, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000082 DASSHJ010000000
VERSION     DASSHJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899784"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFI43_03415"
     CDS             complement(<1..612)
                     /locus_tag="VFI43_03415"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7061208.1"
                     /translation="MTGETRNLLVELLVEELPPKSLKRLSDSFSHSIITSLQIQGLVS
                     EGCEARSFASPRRLAVHIRNVAFHASDKSLSHKLMPVSVGLDKEGQPTPALRKKLETL
                     LRGLHPDFEPNSVFPSLSQSQLKRTFDGKTEALFFESLVKGVSLEEGLQKALNMALDD
                     LPIPKLMSYQLDDGWTTVHFVRPAHGLVALHGAEIVPVSVLGLT"
     gene            complement(609..1514)
                     /gene="glyQ"
                     /locus_tag="VFI43_03420"
     CDS             complement(609..1514)
                     /gene="glyQ"
                     /locus_tag="VFI43_03420"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET7061209.1"
                     /translation="MRTFQEIILSLQQYWDSQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPTRRPKDGRYGENPNRLQHYYQFQVVLKPAPPNILELYLGSLEALGF
                     NLEQNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGMNCKPITGEITY
                     GLERLAMYIQGVENVFDLVWTEGLSYRDVYHQNEVEQSAYNFEHSNTDDLLTAFSRHE
                     KQARYLMEQGLALPAYEHVLKTAHTFNLLDARGAISVTERASYIGRIRELARGVARSY
                     YESRKRRGFPMAPREWVEQLTEDAA"
     gene            complement(1570..3060)
                     /gene="lnt"
                     /locus_tag="VFI43_03425"
     CDS             complement(1570..3060)
                     /gene="lnt"
                     /locus_tag="VFI43_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET7061210.1"
                     /translation="MKLRPETIVSFALGAVTVLGFAPFYLFPVPVFTVALLCHFWRGA
                     VTSGHAAALGFSFGMGMFSAGVTWIYVSLHDFGGMPMAAAVISLFVLCAYLSLFPALV
                     GWLLKKLNIAAPIVWALAAGALWGLSDWLRSVLFTGFPWLTLGYSQAPSSPLAGYAPI
                     LGVYGISLILVSSAALVSLLLEKKFRSRRYLLPLLAIWITGFGLQHIQWTQPQGERVT
                     VSLLQGNISQDLKWRADEIVNSMDTYTRLALGSNSRLIITPEISVPLFRDKVPSSYLA
                     ELASHAKRNDGDILIGMVETPGGGNEYYNSMFSFGASPEQSYRKHHLVPFGEFIPLKF
                     LFGWVADVFPLSDLSRGGLDQRPMNLAGQKVAVNICYEDVFGEEIIKQLPEATLLANV
                     SNDAWFGRSIGPQQHLQISQMRALETGRYMVRATNTGVTAIINERGIVLQAAEIFITT
                     ALNGAVQGYVGETPYIRFGNTLLFALSGSILLIALLPQFRRQGKKR"
     gene            complement(3196..>3828)
                     /locus_tag="VFI43_03430"
     CDS             complement(3196..>3828)
                     /locus_tag="VFI43_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7061211.1"
                     /translation="AKLVKAPEYVETVAGIESVKRAILRTTAGMDRLLEAEQLRHGSA
                     NLDIHTVDLSQLLLEVSQQWQYEAQLKGLELRVEVPDGAKVSSDERLLTLVLQNLLGN
                     AVKYSSTGAINISAGNTSDSGVAVWIFSISDQGPGIPLESLTKLFEAFKRGDTYGKPG
                     VGLGLSIATHAAKLLGGELRVESKVGVGSTFQLVLPTRNSSPVWAVPDTA"
BASE COUNT          890 a         1070 c         1033 g          835 t
ORIGIN      
        1 tgtaagcccg aggacgctga cgggcacgat ttctgcgccg tgcagggcga ccaggccgtg
       61 agcaggccgg acgaaatgta ccgtggtcca tccgtcatcg agctggtagg acatcagctt
      121 cggtatcggc aggtcatcga gtgccatgtt aagcgctttc tgcaatcctt cttcaagcga
      181 cactcctttt acaaggctct cgaaaaacaa cgcctctgtc ttgccatcga atgtgcgctt
      241 gagctgagat tgggataggg atggaaacac agagttcggc tcaaaatcgg ggtgcaagcc
      301 gcggagcagg gtctccaatt ttttgcgaag cgcgggcgta ggctgtcctt ccttatcaag
      361 gcccacgctg acgggcatca gcttgtgcga cagggattta tcgctcgcat ggaaagccac
      421 attcctgata tgaacggcga gccgcctggg tgatgcaaag gatcgagctt cgcagccctc
      481 gctcaccaat ccctgtatct gaaggctcgt aatgatactg tggctgaagc tgtcactgag
      541 ccttttgaga gattttggcg gcaattcctc aacgagcagt tcgacgagca aatttctggt
      601 ttcgcccgtc aagctgcatc ctctgtcaac tgctcgaccc actcacgagg ggccatggga
      661 aaacctcgcc gcttgcggct ctcgtaataa gaccgcgcta caccgcgggc gagttcacga
      721 attcgcccga tatacgacgc ccgttctgta acggagatcg cgccgcgggc atcgagcagg
      781 ttgaaggtgt gcgcggtttt caacacgtgc tcgtaggcag gcaacgcgag cccctgctcc
      841 atgagatatc ttgcctgttt ttcgtgtctg gaaaaggcgg tgagcaagtc atcagtattg
      901 ctgtgctcga aattgtaggc ggactgctca acctcatttt gatggtagac gtcccggtaa
      961 gaaagccctt cggtccagac gaggtcaaac acgttttcca cgccctgtat atacatcgcc
     1021 aggcgctcga gcccataagt gatttccccc gtgatcggct tgcagttcat gccccctact
     1081 tgctgaaagt aggtgaactg cgtgacctcc atgccattca gccaaacttc ccagccgagg
     1141 ccccatgccc caagcgtggg attttcccag tcgtcctcga caaaacggat gtcgttctgc
     1201 tcaagattga agccaagggc ctccagtgac cccaggtaca gttcgaggat gtttggcggc
     1261 gccggcttta acaccacttg aaactggtag taatgctgca accggttggg attttcgccg
     1321 tatcgcccat ccttgggcct gcgtgtcggc tggacataag cagctttcca gggttccggg
     1381 ccaagcgcac gaagaaaagt ggcagtatgt gaagttccgg cccccacctc catgtcgtag
     1441 ggttgcagca aggcgcaccc ttggctgtcc caatactgtt gcagagaaag tatgatttcc
     1501 tgaaatgtgc gcataggaat ccgactgggg aacagtcgat cacgggtggc tgcgtgaaag
     1561 gatgcgattc taccgttttt tgccttggcg gcgaaactgc ggcagcaagg cgatcagcaa
     1621 tattgagccg gaaagtgcaa acaggagggt gtttccaaac cggatgtacg gtgtttcgcc
     1681 gacgtaccct tgcaccgcgc cattcagtgc cgtcgtgatg aatatctcgg ctgcctgcag
     1741 aactattccg cgttcgttga taatagcggt cacgccggta ttggtcgctc gcaccatata
     1801 gcggccggtt tccagtgccc gcatctgtga aatctgcaaa tgctgctgcg gccctatgga
     1861 gcgaccgaac catgcatcat tgctcacatt tgccagcagg gtcgcttccg gtaattgctt
     1921 gatgatctcc tcgccaaaca catcttcata gcagatattc acagcaacct tttgcccagc
     1981 caggttcatg ggcctttgat caagcccccc ccgtgaaagg tctgacaagg ggaatacgtc
     2041 ggcgacccag ccgaacagga attttaacgg aataaattcg ccgaacggca ccagatgatg
     2101 cttgcgatag ctctgctcgg gagaggcgcc aaagctgaac atggaattat agtattcgtt
     2161 gcccccgccg ggtgtttcca ccatcccgat cagaatatca ccgtcattcc gctttgcatg
     2221 gctggcgagc tccgccaggt agctcgatgg gactttgtca cgaaacaggg gaacagaaat
     2281 ttcaggcgtg attatcagcc ggctattgct gcccagcgcg agcctggtat acgtgtccat
     2341 tgaattgacg atctcatccg cgcgccattt caaatcctgc gaaatgtttc cttgcagcag
     2401 gcttaccgtt acccgttctc cttggggttg cgtccattgg atatgttgca agccaaaacc
     2461 tgtgatccag attgcgagca aaggcagcag ataacggcgg ctgcgaaact ttttttcaag
     2521 gagcagggat accagagcgg cgcttgaaac gagaatcagc gaaatgccgt agactccgag
     2581 gatgggcgca tatcccgcca gggggctgga tggggcctgg gagtacccga gggtcaacca
     2641 gggaaaccct gtaaacaaga cgctgcgcag ccaatccgat aaaccccata gcgctcccgc
     2701 ggcgagcgcc caaacgatgg gtgcggcgat attcagtttc ttcagcagcc agcccaccaa
     2761 tgcgggaaat aatgagagat aggcgcatag aacaaacaat gagatcacgg cggccgccat
     2821 gggcattccg ccaaagtcat ggaggcttac gtaaatccag gtcacgcccg cgctgaacat
     2881 gcccatgccg aaggaaaacc ccaaggccgc cgcatgcccg gatgtgactg ccccccgcca
     2941 aaaatggcat agcagggcaa ccgtgaaaac tggaacgggg aaaaggtaaa acggcgcgaa
     3001 accgagaacg gtaaccgcac cgagcgcgaa agaaacgatg gtttccgggc gaagcttgat
     3061 caatgcgctt ggtaaacagt tcttatggct atgccctcag gcttatccct ccttaatacc
     3121 tgttaagctg gccgagagtt taaacgagca atccagcgag aggtaagcag gccagccgaa
     3181 gaggagagat ctgcgtcagg cggtatcagg aacagcccag acaggcgaag aattacgagt
     3241 tggcagtacc agttgaaaag ttgagccaac accgaccttg gattcaaccc tcaattcgcc
     3301 acccaaaagt ttagcggcgt gcgtggcaat gctcaacccc aacccaactc ctggttttcc
     3361 gtacgtatcg ccacgtttga aagcctcgaa cagcttggtc aggctttcga gcgggatgcc
     3421 ggggccctga tcgctaatgc tgaatatcca cacggcaacg ccactgtcgg acgtgttacc
     3481 cgcagagata tttattgcac ccgttgagct gtattttacc gcgttaccga gcaagttctg
     3541 caaaacgagc gtcagcaatc tttcgtcgct tgagactttt gcgccatcag gtacttccac
     3601 ccgcagttcc aatcctttca gttgtgcttc gtactgccat tgctggctaa cctctagcag
     3661 caattgagac agatccacag tatgtatatc gagattagcg gagccatgcc gcaactgctc
     3721 cgcctccagc agcctatcca ttccagccgt tgttcgcaag atggctcgct tgacagattc
     3781 gatacctgcc acggtttcca catactccgg cgccttcacc agctttgc
//
LOCUS       DASSHJ010000083         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_481207, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000083 DASSHJ010000000
VERSION     DASSHJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481207"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFI43_03435"
     CDS             complement(<1..189)
                     /locus_tag="VFI43_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061212.1"
                     /translation="MRLSSNGDPGQAVGADGFFTPTFPFAASGNTPEHGISWADRHTL
                     ADDSGLTECVGRGVVLRTG"
     gene            484..738
                     /locus_tag="VFI43_03440"
     CDS             484..738
                     /locus_tag="VFI43_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HET7061213.1"
                     /translation="MITIQHLLNKIRWDTSYGKGEFQIGYYDRVEQKIHKVPLNNVHL
                     TKDDHFFFHVAEPDGVVHEVPFHRVKEVYKNGKLIWHREH"
     gene            complement(934..1245)
                     /locus_tag="VFI43_03445"
     CDS             complement(934..1245)
                     /locus_tag="VFI43_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HET7061214.1"
                     /translation="MNAQRFTWRGRKNGNPAHDLIAAYLAVDIQKSPAWAKELLQKTD
                     EVKSGQIDSWERFGNAYSLHIFPDRVEIGEDYEEEPEQPVNVPIDDFEAAVEAWLRFI
                     K"
     gene            complement(1242..1790)
                     /locus_tag="VFI43_03450"
     CDS             complement(1242..1790)
                     /locus_tag="VFI43_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HET7061215.1"
                     /translation="MPFLVFPAASVFWLMPLFASAEVDCTGLAQWARLDNGLQVNQKH
                     VFCGEWKGGHPKGFHSRPQGLNPPTVSNFTMQDRPNAAGIYTGKWSHRNYPGRSKFST
                     MFPDGCTAEQILKSISHASANPELNCPAGSPSWVKCGKNRPDFDSKEMLSDYCTNNGE
                     SFLIGFAPARDNRINTAFPIRQ"
     gene            2074..3639
                     /locus_tag="VFI43_03455"
     CDS             2074..3639
                     /locus_tag="VFI43_03455"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7061216.1"
                     /translation="MSLANAYEQYMLELVNAERARAGAQPLALDGDLNEAAENHSAWM
                     IATDTFSHTGTGGSNPGARMTNGGYAFSGSWAWGENIAWMSTRAPAGLQNEVEQMHNN
                     LMNSPGHRANILNPNFREIGIGLEVGQYGSYEGAFVTQDFARSGSNSFLTGVMMRDLD
                     GDKRYDLGEGLGNFTVNARNNATGAIAMTATNPGGGYELELPSGSYTVVFSGSGFAKT
                     TKQVSIGTVNVKLDLINLAASNITPSVSQVQTISGTSGPDVLSGTPAHDVISGLGGDD
                     VLYGNRGNDRLYGGDGNDKLIGGLGADTMSGGAGNDQYFIDNLQDQIIEGPGGGTDLV
                     YTIFDTYTLPGNLENLSTTTTGNRSWTGNELNNWIEGGNGADTLIGLGGNDRLNGLGG
                     NDTLKGGAGADKLDGALGADRLEGGTGNDIFDFDSVSESGLTPGTRDIVVDFVVGQDK
                     LDLSAIDAIAGSGDKAFSLSGANNFTGAGQLRWVNSGTNTIIEGNIDNDLSADFQIML
                     IGNHVLHLTDFVL"
     gene            3928..4593
                     /gene="nfi"
                     /locus_tag="VFI43_03460"
     CDS             3928..4593
                     /gene="nfi"
                     /locus_tag="VFI43_03460"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205208.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HET7061217.1"
                     /translation="MTFAYPHNWRIAPHEAIGVQERLRSLVETSDRFPALHHVAGVDV
                     FFDARCRFMRAAVAVLSFPTLELIASATAQRAVEFPYIPGLFAFRELPAVLDALAQLQ
                     TQPDLILYDGHGIAHPRRFGIASHLGVLVDRPTVGIGKTRLIGREVEPPRERGNWVAL
                     KDGEEVIGAIVRTQTGVKPIYVSIGHRVSLAAAVQVVLACTTRYRLPETTREAHRLAC
                     AYQ"
BASE COUNT         1121 a         1268 c         1237 g         1075 t
ORIGIN      
        1 acctgtccgc aacacaacac ctcgcccaac gcattctgtc aaccctgagt cgtcagcaag
       61 agtatgccta tcggcccagg agattccgtg ctccggcgta ttcccggaag cagcaaatgg
      121 gaaggtcggc gtgaaaaatc cgtcagctcc aacagcctgc cccggatcgc cattggaaga
      181 taatcgcatg ggagcttgcg ttccacgtct acggctatcg tcggcccatc cctcggagaa
      241 tatcacacac ctctcctttt cgatccttca tgctcgcaaa aaagcgatgc taactgcgac
      301 tgcgtggtgg aagtcagcca gatttaacta aactaaactt atttgaacag catcgctttt
      361 cagtctggca gcccggcaag ctccttcaat tcgagggggc ttatacttta agacgtctcg
      421 catcgttggc atctgggcga ccctgattta ttgtgctgcc ggcctgcacc tggagaaaaa
      481 gcaatgatca caatacagca cctactcaac aagatacgct gggatacatc ctacggaaaa
      541 ggtgagttcc agatcggcta ctacgatcgt gtcgaacaaa aaatccacaa ggtgccatta
      601 aacaatgtac accttactaa agatgaccac tttttctttc atgtcgcgga acccgacggc
      661 gtcgttcacg aagtgccctt ccaccgggtc aaggaagttt acaagaatgg caagttgatc
      721 tggcacaggg aacattaaga acgtcgaagg aacaaaacct ggaatactgc ccggaacagg
      781 aaaggatccg ttcaaaggta gttgggatgc tggcagggtt gaagatactc ttcggcgcct
      841 tgttggagta ctgctcacat acttgttcac cggccttggc ctcgaatctg gttgttacga
      901 ttgttacatt tgtccctgtt acgcttatct cttctattta ataaacctca gccacgcctc
      961 aacggctgcc tcaaaatcgt caattggaac gttgaccggt tgctctggct cttcttcata
     1021 atcttccccg atttcgaccc gatccggaaa tatgtgaaga gaataagcat ttccgaagcg
     1081 ttcccatgaa tcgatctgcc ccgatttgac ctcgtccgtt ttttgaagca attcctttgc
     1141 ccatgccgga cttttctgga tatcgactgc aaggtaagcc gctataaggt catgagcagg
     1201 attcccattc ttgcgtccac gccaggtaaa gcgttgcgca ttcattggcg aatgggaaat
     1261 gccgtgttga tcctgttatc ccttgcagga gcaaagccaa tcagaaaaga ctctccattg
     1321 tttgtacagt aatcggataa catttctttc gagtcaaaat ccggcctgtt tttgccacat
     1381 ttcacccagg atggagaacc tgccgggcaa ttgagttccg gattcgcgga agcatgagaa
     1441 atggatttca gaatctgctc cgctgtgcag ccgtcgggaa acatcgtgga aaatttgctc
     1501 ctgccgggat aattcctatg tgaccatttg ccggtgtata tgcctgctgc atttggccgg
     1561 tcctgcatcg taaaattgct tacagttggg gggttcaagc cttgggggcg ggaatgaaac
     1621 cccttgggat gaccgccctt ccattcccca caaaaaacat gcttctggtt gacctgcaga
     1681 ccgttgtcaa gccttgccca ctgggcaaga cctgtgcaat ccacctccgc cgaagcaaaa
     1741 agcggcatca gccaaaaaac agatgccgca ggaaagacaa gaaacggcat cctcatccga
     1801 gggtcatgca gcgagttatt ccttcatcca tacaggagct attccttcat ccggctcata
     1861 gccaagcctg cctttcaggc gggtaattct aaaaaagaat gcttttcatt ccatattcgc
     1921 gcaaaagcac cccttcgaac gtaatacaat actgctgagt tgccaaattg acaactgtcc
     1981 gggataacct ctacgtttcc cctcgataaa aagggccttt ctctcattct tgcccttttt
     2041 aattccgtaa gctaaacaac cggagtttta aacatgtctc tagccaacgc ttacgagcag
     2101 tacatgctcg aactcgttaa cgccgagcgt gcccgcgctg gcgcacaacc cctcgctttg
     2161 gatggcgatc tgaacgaagc agccgagaat cacagcgcct ggatgatcgc aaccgatact
     2221 ttctctcaca ccgggacagg cggatcgaat cccggcgccc gcatgacgaa cggaggttat
     2281 gcgttcagcg gctcctgggc ctggggagaa aacatcgcct ggatgagtac ccgtgctccc
     2341 gcaggcctgc agaatgaagt ggaacaaatg cacaacaatc tgatgaactc tcccggtcac
     2401 cgcgcgaaca tacttaaccc taacttccgc gaaattggca tcggccttga ggtcggacaa
     2461 tatggcagct acgagggagc atttgtcacg caagactttg ctcgttccgg ctcaaattct
     2521 tttctgaccg gcgttatgat gcgcgacctg gacggtgaca agcgctacga cttgggcgaa
     2581 ggactaggca attttactgt taacgcgcgg aataatgcga ccggggcgat agcaatgacg
     2641 gccacgaacc ctggtggggg ttatgaactt gaactcccca gcggaagtta cacagttgtt
     2701 ttttccggca gcggattcgc caagacaacg aagcaggtaa gcatcggaac ggtgaatgta
     2761 aagctggatc tcatcaatct tgccgcaagc aatattacgc cctcggtttc gcaagttcaa
     2821 acgatctctg gcacctcggg cccagacgtt ttgagcggaa cgcctgccca cgatgttatt
     2881 tcgggcctgg gcggggacga cgtcctgtat ggtaacaggg gtaatgacag gctctatggc
     2941 ggcgacggga acgacaagct cattggtggc ctgggtgcag acacgatgag cggaggtgcg
     3001 ggtaatgacc agtatttcat agataacctt caggatcaga ttatcgaggg ccctggtggc
     3061 ggcacagacc ttgtttacac catcttcgac acatacacat tgcccgggaa tctggaaaac
     3121 ctgtcgacga ccaccacggg caaccgcagt tggacaggca acgagcttaa taactggatt
     3181 gagggcggta atggagcgga tacgctgatc gggctgggcg ggaatgatcg actcaacggg
     3241 ctaggcggca acgatacgct caagggtggc gcaggggcgg ataaactgga tggagctctg
     3301 ggcgcggaca ggttggaagg cggtacgggc aatgatattt tcgacttcga tagcgtgagc
     3361 gaaagtggcc ttacgccagg cacccgcgat attgtggttg attttgtggt cgggcaggac
     3421 aagctcgatc tgtcagcaat agacgcaatc gccggatcgg gagataaggc attctcactc
     3481 agtggcgcca ataatttcac cggagccggg caattgcgct gggtcaacag cggtacgaat
     3541 acaatcatcg aaggcaacat agacaacgat ctctctgctg acttccagat tatgctcatc
     3601 ggtaatcacg tgttgcacct gaccgatttt gttctctgaa tcgcattgcc gcgcgagcct
     3661 gcccttcttg gtcgagaggt tgagcggcct tcatctggat aagtgaacgg cttacgctcg
     3721 tgcatgccaa aacgagggaa gcagctgaaa aatcgctgct tccctttttc ttccatttca
     3781 ctcatgcaat acctaaaggc actttgcgga aaagcgacgg ctggggacgc ttcccgaagg
     3841 cccgttcctt catatgcttc ccagggtgat ttaaaaactg gccgagggac gatcctctgg
     3901 caagcgtaac ggacaggagt cctggggatg accttcgctt atccgcacaa ctggcgcatt
     3961 gctccgcatg aagcgatagg cgtccaggaa cgcctgcgtt cgctggttga gacatccgac
     4021 cgcttccccg cgctgcatca cgtcgccggg gttgatgttt ttttcgacgc tcgatgtaga
     4081 ttcatgcgtg cagcagtggc tgtgctttct tttcccaccc tcgagctcat cgcgtctgca
     4141 accgcgcaac gcgccgtgga atttccctat atccccggcc tgtttgcctt ccgcgagctg
     4201 cctgccgtgc tggacgcgct ggcgcaactg cagacgcagc cggacctcat cctttatgat
     4261 ggccatggga ttgcccaccc tcggcgcttc ggcattgcca gccatctggg ggtgttggtc
     4321 gaccgcccca ccgtagggat aggcaaaacg cgtcttattg gacgcgaagt tgaaccgccg
     4381 agggaacgag ggaactgggt tgcgctgaag gatggtgaag aagtgattgg agcgatcgta
     4441 cgcacgcaga ccggagtgaa accgatttat gtgtccattg gccaccgcgt cagcttggcg
     4501 gccgcggttc aggttgtact cgcctgcaca accaggtacc ggctgcccga aaccactcgt
     4561 gaggcacaca ggctcgcctg cgcatatcag tagccgggga gcattcgaag agaacgtcag
     4621 gctggcgctg aaaatgcgca gcgacgcgtt tctctttcac gtactaccct gaacggaaca
     4681 attaacaggg aagcgctact t
//
LOCUS       DASSHJ010000084         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_790088, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000084 DASSHJ010000000
VERSION     DASSHJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790088"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /gene="cysD"
                     /locus_tag="VFI43_03465"
     CDS             <1..519
                     /gene="cysD"
                     /locus_tag="VFI43_03465"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET7061218.1"
                     /translation="CIGGARRDEEKARAKERIFSFRDEFGQWDPKNQRPELWDLYNTR
                     VHPGENIRVFPISNWTELDVWEYIAREELEVPNIYFAHTRDVIRRGSGLLPLSFLVQP
                     KEGEQVENLMVRFRTVGDMSCTCPVESSAVSVGEIIAETAITRITERGATRMDDQTSE
                     ASMELRKKEGYF"
     gene            789..2072
                     /gene="cysN"
                     /locus_tag="VFI43_03470"
     CDS             789..2072
                     /gene="cysN"
                     /locus_tag="VFI43_03470"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HET7061219.1"
                     /translation="MSAIEKISFDHTQLLRFITAGSVDDGKSTLIGRLLHDSKSIFED
                     QLSAISHTSRRRGMEAVDLSLLTDGLQAEREQGITIDVAYRYFATPKRKFIIADTPGH
                     EQYTRNMVTGASTANLAIILIDARKGVLTQSRRHAYLASLVGIPHLVVAVNKMDLVSY
                     SREVFDRICADFASFAEGLTLRNIAYIPLSALHGDMVVERGDNLGWYQGPTLMDLLEN
                     VPIDHDINLEDFRFPVQWVCRPQTEELHDFRGYMGRIDSGFINVGDEVKVLPSGLTSR
                     IKEIVTYDGFLDQAVAPQSVTLTIEDHLDISRGDMLVKAAEAPQVTREFEAILCWLSE
                     HSLDLTRKYLIKQTTRTVRALVSRIGYRVDVNTLEREPVETLKMNDIGRVGFKVQQPL
                     VCDPYPRNHATGSFIVVDEINNNTIAAGMICAPES"
     gene            2309..>2993
                     /locus_tag="VFI43_03475"
     CDS             2309..>2993
                     /locus_tag="VFI43_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HET7061220.1"
                     /translation="MTSQSSVPPSASTAAVMSPPLGFGVAFADLYRREGLVRLDRAFL
                     HFLQEGEPALRARLDHARANPDNLDRKAEAALLIEVAPWMEDFIARLFGIEKEVAELA
                     ARHHALAPLYSCKRQFVQRRAMNKVSDAEAASVDGLALEQVLSAEFQSPFSELAFANH
                     VTNWLSDESANEERLKAALRYAAWALKTPEGRERNRDGVLFKAPAKLDFLNLLDTDSD
                     SSAGYTVHRL"
BASE COUNT          658 a          804 c          837 g          694 t
ORIGIN      
        1 tgcataggcg gcgcgcgccg tgacgaggag aaggcacggg cgaaggaacg aatattttcg
       61 ttcagggatg aattcggcca atgggatccg aagaatcagc gtccggaact gtgggatctc
      121 tacaacaccc gcgttcatcc gggggagaac attcgtgtgt ttcctatcag caattggacc
      181 gaactcgacg tctgggaata catcgcccgc gaggaactgg aggttccgaa catctatttc
      241 gcccataccc gcgatgtaat caggcgcggt agcggcctcc taccgctgtc tttcctggta
      301 cagcccaagg aaggggagca ggtggaaaat ctcatggttc gtttccgcac tgtgggcgac
      361 atgagttgca cctgccccgt ggagtcgagt gcggtcagcg ttggagaaat cattgccgaa
      421 accgcgatca caaggatcac cgagcggggc gcaacgcgga tggacgatca gacatctgaa
      481 gcatccatgg agttgcgcaa gaaagagggc tacttctgaa gcatgctgcg atcgaagtct
      541 cgctgtggcc ataaaggtcg cggtagaaac ccattttcgt tcgggaattc gccatacgag
      601 cactgaattc aggatacccc atacagcagc ctgtccgtta ttcctctggt ttcaccttag
      661 catcactcgt cggcagatca tgaggatcgg gggtctacga taatgattgt tcccgcagca
      721 ggcgatgtta cgcgacattc aggatttgat gtgacattca ggatttgata agagttaagt
      781 gaatataaat gtcagctatt gaaaagattt ccttcgatca tacgcagttg ttgcgcttca
      841 taactgcggg gagcgtggat gatggcaaga gcaccttgat cgggcgtttg ctgcatgatt
      901 cgaaatcgat tttcgaagac cagttgagcg ccatctcgca tacgtcccgc cggcgcggga
      961 tggaggcagt cgatctctcc ctgcttacag atggcttgca agcggaacgt gagcaaggga
     1021 ttaccatcga tgtggcttac cgttattttg ccactcccaa gcgtaaattc atcattgccg
     1081 atacgcccgg ccatgagcaa tatacccgca acatggtgac aggtgcttcc accgccaatc
     1141 ttgccattat tctcattgac gcgcgcaaag gcgtactgac gcaatcgcgg cgtcatgcct
     1201 atctcgcgag cctcgttggc atccctcacc tggtggttgc cgtcaacaaa atggacctgg
     1261 tgtcctattc acgcgaggta ttcgatcgta tctgcgcaga ctttgcctcc tttgccgaag
     1321 gcctcacgct gcgcaacatc gcatatattc cgttgtccgc gctgcacggc gacatggtgg
     1381 tcgagcgcgg ggataatctc ggctggtatc agggtccaac actgatggat ctgctggaga
     1441 acgtgcctat tgatcacgac atcaatcttg aggatttccg atttcccgtt cagtgggttt
     1501 gccgtcctca aaccgaggag ctgcatgact tccgtggcta catgggacgt atcgattccg
     1561 gcttcatcaa tgtaggggat gaggtgaagg tgctgccttc gggcctcacc tcccgcatca
     1621 aggagattgt tacgtatgac ggcttccttg atcaggctgt cgcgccgcaa tccgtgacgc
     1681 ttacgatcga agatcacctg gacatctcac ggggggacat gttggtaaaa gcggctgaag
     1741 ctccacaggt cacgcgggag tttgaggcaa tactgtgctg gctatccgag cacagcctcg
     1801 acctcaccag gaagtatctc atcaagcaaa ccacgagaac cgtcagggcg ctggtctccc
     1861 gcatcggcta tcgcgtggac gtcaataccc tcgagcgtga accggtggag acactgaaaa
     1921 tgaatgatat cggcagggtg ggcttcaagg tgcagcaacc tctcgtctgc gatccctatc
     1981 cgcgcaatca tgcgaccggc agcttcatcg tggttgacga aatcaacaat aacaccattg
     2041 ccgcaggaat gatttgtgcg ccggaaagct gatctggcgg taatccgact actggatatc
     2101 gatttctgca tatcgcgttt cacgggcgtc gacaaggcta tcggcctcgc ggcttttgca
     2161 tttttgcttc ctgaacggcc ttgagacaac ctgccagaga acggatattc cggaagcagt
     2221 cagaacttta atcacagttt tgctctaacg catcgatagc gactttttga cttacccttt
     2281 tgactcaccc ttatccgcga caaccatcat gacaagtcaa tcatcggttc caccttctgc
     2341 ttcaacagct gcggtgatgt cccccccgct tggattcggc gtggctttcg ccgacctgta
     2401 tcgccgcgag gggctggtca ggctggatcg ggctttcctg catttcctgc aggagggaga
     2461 gcctgctctt cgagcccgtc tcgaccatgc gcgtgccaac cctgacaatc tggaccgcaa
     2521 ggcggaagcc gccctgctga ttgaagttgc tccgtggatg gaagatttca ttgcccggtt
     2581 gtttggcatc gagaaggagg ttgccgagct tgctgcgcgt caccacgcgc ttgctccgct
     2641 ttattcctgc aagcggcagt ttgttcagag gcgggcaatg aacaaggtaa gcgatgcgga
     2701 agcagcgtcg gtcgacgggc tggcgctgga acaagtattg tctgccgaat ttcagtcacc
     2761 tttttccgaa ctggcttttg ccaaccacgt gacaaactgg ctatcggacg agagcgcgaa
     2821 cgaagagcgg cttaaggcgg cccttcgcta tgccgcctgg gcgctgaaaa cgccggaagg
     2881 acgggagcgc aaccgggacg gggtattgtt caaggcgcct gcgaagctgg atttcctcaa
     2941 tctattggat actgatagcg acagctctgc cggttataca gttcaccgcc ttc
//
LOCUS       DASSHJ010000085         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_842956, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000085 DASSHJ010000000
VERSION     DASSHJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842956"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFI43_03480"
     CDS             <1..792
                     /locus_tag="VFI43_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET7061221.1"
                     /translation="LDVIVENFSVGEKHLGQLELLANHQEQNWRIEKLHLVTADSSLS
                     IKGLWDRPEKREDASRIQATLFLESNDIGKFLTRLGLPERIKRGRGRLEGSFSWNGVP
                     QSIDYATLTGSFRITARNGQFPKFEPGIGRLFGIFDLRALPRRIILDFHDIFSEGFGF
                     NEILADIKVNRGIAATNDLVIEGPAARIMMTGRINLEEESQDLHMKVVPSLGLATPVV
                     GVASLIVSKALQKPASPNEYHITGEWTDPLVTKIAGRAARLDERD"
     gene            1020..1859
                     /locus_tag="VFI43_03485"
     CDS             1020..1859
                     /locus_tag="VFI43_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7061222.1"
                     /translation="MSAISSEGKPGPVRVAAIQMTAGPDIGANLTEAERLLDAAASRG
                     AHLAALPEYFCLMGMKDSDRLAAREEDNCGPIQQFLSETSRRLGLWIIGGSVPLAALR
                     HDKIRNSCLVYDNEGKRVARYDKMHLFGFELGAERYAEEETIEAGSGVVALESPFGRI
                     GLSICYDLRFPELYRSMGKVDIIFAPAAFTATTGKAHWETLIRARAIENLAYVVAPAQ
                     SGRHSNGRETHGNSMIVDPWGVVLDRLADGAGVVIADINPEYQASLRNSLPALRHRIL
                     HHC"
     gene            1924..3384
                     /gene="tldD"
                     /locus_tag="VFI43_03490"
     CDS             1924..3384
                     /gene="tldD"
                     /locus_tag="VFI43_03490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HET7061223.1"
                     /translation="MTTSQIIQPADLFSTANNCLLTPYEIDTAALQNVFGEILTHRVE
                     YADIYFQYNRAEGWALEEGIVKSGSFNIEQGVGVRAVSGDKTAFAYSDDISMPALVSA
                     AQATRAIARDGANRSVPAVRRSRGRELYLPHDPISSLKDTEKVALLEKLEGYARARDK
                     RVIQVMASLSGEYEVVLIARSDGLLAADVRPLVRLSLQVIAEENGNLEQGVAGGGGRF
                     DYGYFTDELLRDYAAKAVDQALVNLRAKPAPAGTMTVVLGAGWPGILLHEAIGHGLEG
                     DFNRKGSSAFSGRIGERVAAEGVTVVDDGTLARRRGSLNVDDEGNPTQCTVLIENGIL
                     KGYLQDSLNARLMNVPVTGNARRESFAHVPMPRMTNTYMLNGDKTPEEIIASVKHGLY
                     AVNFGGGQVDITSGKFVFSAAEAYMIEDGKISYPVKGATLIGNGPDVLTRVSMIGNDM
                     SLDPGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTA"
BASE COUNT          805 a          892 c          981 g          754 t
ORIGIN      
        1 cttgacgtca tagtggaaaa tttttccgtt ggtgaaaagc acttgggtca attggaatta
       61 ttggcgaacc atcaggaaca gaactggcgt atcgaaaaac tgcacctggt cactgccgat
      121 agttccctat caataaaagg attatgggac aggccggaaa aacgtgaaga cgcatcgcgc
      181 atacaagcca cacttttcct ggagtcgaat gatattggaa aattcctgac gcgcctcggc
      241 cttccggaac gcatcaaacg cggcagagga aggcttgagg gaagcttttc ctggaatggc
      301 gtgccccaat caatcgacta tgcgacgctg accggcagtt tcaggattac cgcccggaac
      361 ggccagtttc cgaaatttga gccgggaatc ggacggctgt tcggaatttt tgatctaaga
      421 gccctgcccc gcaggataat actcgatttt cacgatatct tcagtgaagg ctttggtttt
      481 aacgaaatct tggcggatat aaaggtcaac cgcggtattg cggccacgaa tgatctggtc
      541 atcgaagggc ccgcggcaag gataatgatg accggcagga taaatctgga ggaggaaagc
      601 caggacctcc acatgaaagt tgtcccctcc cttggattgg caaccccggt ggtaggcgtt
      661 gcttcgctga ttgtaagcaa agcgctgcaa aaaccagctt cacccaatga ataccatatt
      721 accggcgagt ggaccgatcc gttggttacc aagatcgcgg gacgggcggc ccgcctcgac
      781 gaacgcgatt aacttcccct ggacgccccg gccagcccct gttttcaaga aaagcaagcg
      841 ggtaagaacg aaagcggagg cgtcatgtct accctttcac cttgaagtat gagaagcttt
      901 gcctctgcct gtttcctttg tctgtaaaaa cggacttctt atagcatgac ggaaccctta
      961 tcgcagattt gcacagaccg gaataaatcg tgactacgct aaaagatccg agtacttgca
     1021 tatccgcgat atccagcgaa ggaaaacctg gtcccgtgcg tgtggccgcc atacaaatga
     1081 ccgcaggtcc tgatatcggc gctaacctga cggaagcaga gcgcctgctt gacgccgccg
     1141 cttctcgcgg agcacacctg gctgcacttc ctgaatattt ctgcctgatg ggaatgaagg
     1201 attctgacag gcttgccgcg cgggaagaag ataactgcgg acccatacag caattcctca
     1261 gcgaaacctc tagacgtctc ggactttgga tcatcggcgg ttcagttccc ctggcggccc
     1321 tgaggcacga taagatacga aacagctgcc tcgtttatga taacgaggga aagcgggttg
     1381 ctcgttacga caaaatgcat ctgtttggct ttgagcttgg cgccgagcgt tatgccgaag
     1441 aagaaacgat cgaagcgggc tccggagtcg ttgcccttga atcccctttt ggccgcatcg
     1501 gtctctccat ttgttatgat ctccgtttcc cggagcttta ccgttcgatg ggtaaagtgg
     1561 acatcatttt cgctccagcg gctttcacgg caaccaccgg aaaggcgcac tgggaaaccc
     1621 tcattcgtgc ccgggcgata gaaaatctcg catacgtggt cgcgccagct cagtccggac
     1681 ggcattcgaa cgggcgggaa acgcatggga acagcatgat tgtcgaccca tggggagtag
     1741 tgcttgatcg gctcgctgat ggagcagggg tggtgatcgc agatatcaat cccgagtatc
     1801 aggcaagcct gcgcaacagt ttacccgcat tgaggcatcg aatcctgcat cactgttgat
     1861 cctgaaccct ttagtccgac cctttccgac ttcttgccaa ttgctttcat ttaaagactg
     1921 atcatgacta catcccaaat catccagccc gccgatcttt tttccactgc caacaattgt
     1981 ttgctcacgc catacgagat cgatacggcg gcactacaga acgtatttgg agaaatactt
     2041 acgcaccgcg tcgagtatgc cgacatctat tttcagtata accgggcgga aggctgggcc
     2101 ctagaggaag gtatcgtcaa atcggggagc ttcaatattg agcagggcgt cggcgtgcgt
     2161 gcggtgagcg gtgacaagac cgcttttgcc tactcggatg atatcagcat gcccgcgctc
     2221 gtttctgctg cgcaagccac ccgcgcgatt gcccgggacg gcgccaaccg gtcggtcccg
     2281 gcagtcaggc gcagcagggg gcgagagctt tatcttcccc acgatccgat ctcgagtctc
     2341 aaggacacgg agaaagttgc gctgctcgag aaacttgaag gctatgcgcg tgcccgtgac
     2401 aagcgcgtga tccaggttat ggcatcgctt tccggcgaat atgaagtcgt gctgattgcg
     2461 cgcagcgacg gcctcctggc cgccgatgtc cgccccctgg tgcggctttc gttacaagtg
     2521 atcgccgaag agaacggtaa tcttgagcaa ggggttgccg gaggaggagg acgctttgat
     2581 tatggctatt tcaccgatga gttgctgcgc gattatgctg caaaggcagt ggatcaggcc
     2641 cttgtgaatc tgagggcaaa gcccgcgcca gccggaacca tgacggtggt gctgggcgcg
     2701 ggttggccgg gaatactttt gcatgaggcg atcgggcacg gactggaagg tgatttcaac
     2761 cgtaagggaa gttctgcatt ttccggacgg atcggcgagc gtgtagcggc agaaggcgta
     2821 actgtagtgg atgatggcac gcttgcacga cgacgcggtt cactgaacgt ggatgacgag
     2881 gggaatccaa ctcagtgcac ggtactgatc gaaaatggca ttctcaaggg ctatcttcag
     2941 gacagcttga acgcccggct gatgaatgtt ccggtcacag gcaacgcgcg acgggaatca
     3001 tttgcccatg tgcccatgcc gcgcatgaca aatacttata tgctgaatgg ggacaagaca
     3061 ccggaagaaa tcattgcgtc ggtcaagcac gggttatacg cggtgaattt cgggggtggc
     3121 caggtcgata tcacaagcgg aaaatttgtc ttttccgcgg ctgaggccta tatgatcgaa
     3181 gacgggaaaa tctcctaccc cgtgaaaggc gcgacgctca tcggtaacgg cccggacgtg
     3241 ttgacccgtg tatcgatgat cggcaatgat atgtcgcttg accctggcgt ggggacatgc
     3301 ggcaaggaag gtcaaagcgt gccagtgggt gtgggacaac caacccttcg tatcgacgga
     3361 ctgacggttg gagggactgc ctagccctta ttgaacgctt gcattctcga tacgggtacc
     3421 agccagcctg tc
//
LOCUS       DASSHJ010000086         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1230976,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000086 DASSHJ010000000
VERSION     DASSHJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230976"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFI43_03495"
     CDS             <1..684
                     /locus_tag="VFI43_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET7061224.1"
                     /translation="WSTIVAVIVVTIMVQLGNWQLSRAREKESRQEQLDVLSRQPAVT
                     IPPTPVKLEDFQYRKVEASGFYVPDHTIYLDNKIYRGAAGYHVITPLRIGGSDMHVLV
                     NRGWVAANRDRSKLPEVPTPDGPVDISGVATTATQKTLELSDELVSGRVWENLNLERY
                     RAATGLALQPVMILQQDDVKDGLIREWPRPDIGIGRNLGYALQWFAMAAAVSIIYLVL
                     SVKRERTGS"
     gene            728..1273
                     /locus_tag="VFI43_03500"
     CDS             728..1273
                     /locus_tag="VFI43_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061225.1"
                     /translation="MVAPIVASYLLYFSDVRPGSVNYGELLEVKPLTGNALNQSDKTI
                     FRIRQLRGKWALISVDSGRCDEMCRKKLYYMRQVRTIQNTEKERIERVWLIDDDQTPS
                     ADVLKEYPGIWLINAKDSDLLKELPAKESIHDHIYVVDPIGNLMMRFPKNPDPAQMAK
                     DIKRLLKVSQLEHAMGTDKRH"
     gene            1378..2274
                     /gene="cyoE"
                     /locus_tag="VFI43_03505"
     CDS             1378..2274
                     /gene="cyoE"
                     /locus_tag="VFI43_03505"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET7061226.1"
                     /translation="MATTSIVSQQTASRVQQFYRLTKPRVVSLIVFTAVIGMFLAVPG
                     AVPLDKLIFATVGIAFVAGAAAAVNCLVEQKIDSVMARTRGRPLPRGTVTSPETLFFL
                     ALLGGTGLLMLHQLVNPLTMWLTLGTFVGYAIIYTVLLKPMTPQNIVIGGASGAMPPV
                     LGWAAMTGEVSSDALLLFLIIFAWTPPHFWALALYRKLEYAKVGMPMLPVTHGDKFTR
                     LHVLLYTFILIAVTLMPYATQMSGLIYLIGAGVLDAVFLYYAIKIYVDYSDQLARRTF
                     RYSILYLAALFAVLLIDHYIRF"
     gene            2275..2898
                     /locus_tag="VFI43_03510"
     CDS             2275..2898
                     /locus_tag="VFI43_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7061227.1"
                     /translation="MFRVLGEGWRAVVLGAAVMFIAACGNTSTPPSTPAFASTDITGV
                     QFGKGFTLIDHNGVVRSLSDFRGKAVALFFGYTHCPDKCPATMAQVAAALEKLGPAAG
                     RVQVLFVTLDPERDTPSVLKQYLSGFNPRFLGLYGDDETTQETASQFKVFSQRQGAES
                     SGDHTVDHSTGVYIFDPQGQLRLYANAISDGGDALARDLAQLLKAAA"
     gene            complement(2932..>3358)
                     /locus_tag="VFI43_03515"
     CDS             complement(2932..>3358)
                     /locus_tag="VFI43_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7061228.1"
                     /translation="LWRARKTLSPLLRDIAPKKINEDVVVPVSMLPRFLEEVSSLSRE
                     FQVANVNFGHAGNGNIHVNLLVNPDIPEEMRRAEQCLEKIFDLVIQLDGTLSGEHGVG
                     SEKRAFVGKEIDATTMKLMKGIKQVFDPENILNPGKMFP"
BASE COUNT          761 a          884 c          914 g          799 t
ORIGIN      
        1 tggtcaacaa tagttgcggt gattgtcgta acgatcatgg tgcagcttgg caactggcag
       61 ctctcccgtg ccagggaaaa ggagtcgcgg caggaacagc tcgacgtgct ctcccggcag
      121 cctgcggtaa caattcctcc tactcccgtc aagctggagg attttcagta caggaaggtg
      181 gaagcaagcg gtttttacgt accggatcac acgatctatc tcgataacaa gatttacaga
      241 ggggcagcgg gctaccacgt tataaccccc ctgaggatag gcggcagcga catgcatgtc
      301 ctggtcaacc gcgggtgggt ggcggcaaat cgggaccgaa gcaagctacc cgaggtaccg
      361 acacctgacg gtccggttga tatttcggga gtggcgacga cggcgacgca aaagacactg
      421 gagctgtcag atgagcttgt atcaggcagg gtctgggaaa acctgaatct cgaacgctat
      481 cgtgctgcaa caggcctcgc ccttcagcct gtaatgattt tgcagcagga cgatgtcaag
      541 gacgggttga tccgggaatg gccccgaccg gacatcggga tcgggaggaa tctcggttac
      601 gcgcttcagt ggttcgcgat ggcagccgca gtatcgatta tttatctggt gttaagtgtt
      661 aaacgagagc gaacaggcag ctaacaggaa gaagaacagg cggacgctga tcttgttggt
      721 ggtcgtgatg gttgcaccga tagttgcgtc ttatttgctt tatttttcag acgtaagacc
      781 aggtagcgtc aactatggag agctgctgga ggtgaaaccg ctgacgggca atgctttaaa
      841 ccagtcggat aaaaccattt tccggatacg gcagctgcgg ggcaaatggg cactcatctc
      901 ggtagattcg gggcgatgcg acgaaatgtg ccgcaagaag ctctactata tgcggcaggt
      961 tcgaaccatt cagaacactg aaaaggaaag aatagagcgg gtatggctga tcgacgacga
     1021 tcagacaccc tcggcggacg tacttaagga gtatcccgga atttggttga tcaacgccaa
     1081 ggatagcgac ctgctcaagg aattacctgc aaaagaatct attcatgatc atatttatgt
     1141 cgtcgatccg atcgggaatc tcatgatgcg cttcccgaaa aacccggacc cggcgcaaat
     1201 ggcaaaagat ataaagcgct tgctaaaggt atcccagctt gagcatgcga tgggaactga
     1261 caagaggcac taacggaatc ggcattgcga gagacccctc ttgcgagagc gatagctaaa
     1321 gaatgtatcg gtagttgcag aacgaaaatc ctgcaatgaa acaaaggaga taaaagcatg
     1381 gctacgacca gtattgtttc gcagcaaacc gcctcccgtg tgcaacagtt ttatcgtctg
     1441 acgaagccca gggtggtttc gctgatcgtg tttacggccg ttattggcat gtttctggcc
     1501 gtacccgggg ctgttcccct ggataagctg atattcgcga ccgtcggtat cgcttttgta
     1561 gcgggcgccg ccgcggcggt aaactgcctg gtggagcaaa agatcgactc tgtcatggcg
     1621 cgaacacgcg gcaggcccct gccgcgtggt acggtgactt cgcccgaaac gctgttcttt
     1681 ctcgcactgc tcgggggcac gggcttgctg atgctgcacc agttggtcaa tccgctgacc
     1741 atgtggctga ctttgggtac ctttgttgga tatgcaatca tctacaccgt attgctgaaa
     1801 ccgatgacgc ctcagaacat agtcataggg ggagcctcag gtgcaatgcc gccagtgctg
     1861 ggttgggcgg caatgacagg tgaagtttcc tctgatgccc tacttctgtt cctgatcatt
     1921 tttgcatgga ccccgcccca tttctgggca ttggcgctct accgcaagct cgagtacgca
     1981 aaagtcggca tgccgatgct gcctgttacg catggcgata aattcactcg cctgcatgtc
     2041 ctgctttata ccttcatact cattgcggta acgttgatgc cctacgcgac gcaaatgagc
     2101 gggctcattt atctgatcgg cgccggggtg ctcgatgcgg tatttttgta ttacgcaata
     2161 aaaatctatg ttgactatag cgatcagctt gcgagaagga cattccgcta ctcgattcta
     2221 tatctcgcag cgctatttgc cgtactgttg atcgatcact acatccgttt ctaagtgttc
     2281 cgcgttctcg gcgagggctg gcgggcagtc gtattgggag cggcggtaat gtttatcgct
     2341 gcctgcggaa atacctctac gcctccgtca accccagcct ttgcttccac cgatattact
     2401 ggtgttcaat ttggcaaggg attcacgctc atcgatcaca atggcgtggt tcgaagtctt
     2461 tcagacttca ggggaaaggc cgtagcgctc tttttcggct acacccattg cccggacaag
     2521 tgtccggcga caatggccca ggttgcggcc gccctggaaa aacttggacc ggccgccggg
     2581 cgcgtgcagg ttttatttgt caccctcgat ccagagcgcg atactccctc cgttttaaag
     2641 caatacctct ccggtttcaa tccccggttc ctcgggcttt atggggacga tgagaccacc
     2701 caagaaactg ccagccagtt taaagtcttt tctcagaggc agggtgccga gtcttccggc
     2761 gaccataccg tggatcattc cactggagtc tatatttttg atccccaggg gcagcttcgc
     2821 ctgtacgcca acgcgataag cgacggtggc gacgcccttg cgcgggacct ggctcaattg
     2881 ctaaaggcag ccgcttgacc aaattgcgct gcatcacgtt cccgcatgcc ctcatggaaa
     2941 catcttccct ggattcagaa tattttccgg atcgaatacc tgcttgatac ctttcatcag
     3001 cttcattgtc gtagcatcga tctccttccc gacgaatgcg cgcttctcgc ttcctacgcc
     3061 atgctcgcca gataaagtcc catccagctg gatcaccagg tcgaatatct tttccaggca
     3121 ttgctctgcg cggcgcatct cttcgggaat atcgggattc accagcaggt tgacgtggat
     3181 attcccgttt ccggcatggc cgaaattcac attcgccacc tgaaattcac ggctcaaact
     3241 gctcacttct tcaaggaagc gcggcagcat cgatacaggc acgactacat cctcattgat
     3301 tttcttcggg gcgatatccc ttaatagcgg cgatagggtt ttacgagcgc gccaaagc
//
LOCUS       DASSHJ010000087         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_206645, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000087 DASSHJ010000000
VERSION     DASSHJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206645"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFI43_03520"
     CDS             complement(<1..303)
                     /locus_tag="VFI43_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET7061229.1"
                     /translation="MLLIAHRGYHAMVPENTLAAFEAAVSIGANGIETDVRFSRDGLP
                     VLIHDRLTRTGEPVAELTRAEIEKISGHEIPTLEEALERFPGILWNIEIKTPVALPA"
     gene            complement(377..1192)
                     /locus_tag="VFI43_03525"
     CDS             complement(377..1192)
                     /locus_tag="VFI43_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7061230.1"
                     /translation="MLLISSVLAPIAYLAFAAVVYFAQPSLLYYPETGRDIVATPDAV
                     GLDYEAVEIDTSDGETLQGWFVPAPGASRTVLFLHGNAGNISHRMDYLSMFHSLGYST
                     FIIDYRGYGASSGTPSEQGTYLDAEAAWQYLTEYRNIPPERIILFGESLGGAIAAWLA
                     VREKPGALVLASSFTSVPDMAAKLFPILPVRFLSRFEYDTLKSLKSVSCPVFIAHSPE
                     DEIVPYVQGRALYAAAPEPKQFLQLQGGHNQGLIFVRKEWADELDRFIRTNIK"
     gene            1302..1850
                     /gene="ppa"
                     /locus_tag="VFI43_03530"
     CDS             1302..1850
                     /gene="ppa"
                     /locus_tag="VFI43_03530"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7061231.1"
                     /translation="MNLDRVNSGSDVPNDFNVIIEIPMNADPIKYEVDKETGAMFVDR
                     FMSTSMHYPCNYGYIPHTLSEDGDPVDVLVISPVPLISGVVVRCRPLGMLQMSDEAGE
                     DAKVLAVPIDKLCNLYLNIKSYEDLPELVRSQTAHFFEHYKDLEPGKWVKINGWAGVK
                     EAKAEIEGGVARYKSAKKKPMF"
     gene            1902..>2124
                     /locus_tag="VFI43_03535"
     CDS             1902..>2124
                     /locus_tag="VFI43_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7061232.1"
                     /translation="MSWLEPIKLEGSVVTLEPLEMDHVEPLKAAVADGEYWKLWFANV
                     PSPDQMENYVTKAIENAKSGDLAFAVKLNN"
BASE COUNT          545 a          536 c          577 g          466 t
ORIGIN      
        1 cgcgggcaac gcaaccggcg ttttgatctc gatattccat aaaatgcccg gaaagcgttc
       61 gagtgcttcc tccaatgtag gtatttcatg acctgatatt ttttcaattt cggctcgagt
      121 aagctcggcc accggctcgc ccgttcgggt aagccgatca tgtatcaata ccggcagacc
      181 gtcgcggctg aagcgcacat ccgtttcaat cccgttggcc cctattgata cggctgcttc
      241 gaatgccgcc aaggtattct cgggaaccat ggcatgatag ccgcgatgag caatcagcag
      301 cattggcgaa ttggaaactt gctgtaagcg cctaaaccat tggctcgttt gcaagctgca
      361 tgcataaccg cgcgcatcac ttgatatttg tgcgaatgaa cctatccagt tcgtcagccc
      421 attccttgcg cacaaaaatg agaccctgat tatgcccgcc ctgtagctgc agaaactgct
      481 ttggctcagg ggcagccgcg tataacgccc ggccctgcac atatgggacg atttcatcct
      541 ccgggctatg cgcaatgaac acagggcatg aaacggactt cagcgacttc agcgtgtcgt
      601 attcgaagcg ggacaaaaac cttactggca ggatagggaa gagttttgca gccatgtcag
      661 gcactgaggt aaacgaagag gccagcacaa gcgcgccggg tttttcccgc acggcgagcc
      721 aggcggcaat ggcgcctccc agtgattccc caaacaatat gattcgctcc ggagggatat
      781 ttctgtattc agtcaggtat tgccaggcgg cctctgcatc gagataagtc ccttgctccg
      841 aaggtgttcc gctgctggcg ccgtagcccc gatagtcgat aataaaagtg ctgtagccca
      901 gcgagtggaa catcgaaagg tagtccatgc ggtgagaaat attgccggcg tttccgtgaa
      961 gaaacagcac ggttcgactt gcaccaggag caggcacgaa ccagccctgg agtgtttcac
     1021 catcggaggt gtcgatttcc accgcctcgt aatcgagccc tacagcatcg ggagttgcaa
     1081 caatgtctcg cccggtttca ggataataga gaaggctcgg ttgggcgaaa taaacaacgg
     1141 ctgcgaaagc gagataagcg atcggagcca gaacggatga aatgagtagc atgctcacgt
     1201 tgaaatggag ttggattggt atttgatcca ggcccgggaa ttggtgaatc cggcgggctc
     1261 aaagttataa tacgcaactt tcaaccagga acaggagcat aatgaatctc gaccgggtta
     1321 actctggcag cgacgtccct aacgatttca acgtcatcat agaaataccg atgaatgccg
     1381 atcccatcaa gtacgaggtg gacaaggaga caggcgcgat gttcgtggat cgcttcatgt
     1441 cgacgtcgat gcactacccc tgcaactacg gctatattcc tcataccctg tctgaagacg
     1501 gcgatccggt tgacgttctg gtaatttctc ccgtccctct catttccggc gtggtggtgc
     1561 gctgcaggcc gctcggaatg ctccagatga gtgatgaagc gggtgaagat gccaaggtcc
     1621 tggcggtgcc catcgacaag ctctgcaatc tttatctcaa tataaaatct tatgaggacc
     1681 tgcccgagct ggtacgttcc cagaccgcgc attttttcga gcactacaaa gacctggaac
     1741 ccggcaaatg ggtaaagatc aacggatggg ctggcgtcaa ggaagcaaaa gccgagattg
     1801 aaggcggtgt cgcgcgctac aagtcggcga agaagaagcc aatgttctaa gactgggttt
     1861 tagccaagct taattgaacg attgaaagca agagtatcag aatgagctgg cttgagccaa
     1921 taaaactgga gggaagcgtt gtaacacttg agccactcga aatggaccac gtagagcctc
     1981 tgaaggctgc ggtagcggat ggcgagtact ggaagctatg gtttgccaat gtcccctctc
     2041 cggatcagat ggaaaactat gtgaccaagg ccatcgaaaa tgcaaaaagt ggagatctcg
     2101 cgtttgcagt aaaactcaat aata
//
LOCUS       DASSHJ010000088        11997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_353276, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000088 DASSHJ010000000
VERSION     DASSHJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353276"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFI43_03540"
     CDS             complement(<1..742)
                     /locus_tag="VFI43_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HET7061233.1"
                     /translation="MKPAFWLLVLFAVAVAATLAAKNNGYILIVAQPYRIELSLNLMV
                     LLLLLAFFVLYFFVRLAAMTLRLPAEVGEFRLRRRREKAHKAMLEGFKALLERRYDKA
                     EKASAAALEMKESPVVNALNAVVAARAAHEQGRYLERDEFIALAEADAPKETALRLMT
                     KAELLLDEGRSEEALHVLQILHSVEKRMPEAALWLEIKVQQQLGNWKEVLNLLGQLEQ
                     HNAVDKPILSRLRRHAHLENMENMQDAEA"
     gene            complement(739..1821)
                     /locus_tag="VFI43_03545"
     CDS             complement(739..1821)
                     /locus_tag="VFI43_03545"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET7061234.1"
                     /translation="MSSQAEPVETNPDPEESREGRKAASDGVWKTLRARATPVWFLAV
                     FFAFLLAWQWYEAHDQISEVKHDLAKRLAEADASGKESRALAKGAVEAGRRTETKVGL
                     LEARLAESQSQQLALEELYQELTRNRDETTLEEVEQLLLIANQQLQLASNVKAALIAM
                     QEADARLQRIGRPQLTPLRKVLAADMDLLRAAPYTDTVGISIRLDNLAAAVEKLPLAM
                     ELRPPEPPVSREQPPAENPWLRFVRQTWEDLKQLVRIQNMDKPDVALLSPSQAYFLRE
                     NLKLRLLSARNALLARDGTSFNTDIDTASDWIKRYYDTQSAPVIRVLEMLRELRGNEV
                     GVELPRISASLDAVRNYRLGRDRGNR"
     gene            complement(1818..2621)
                     /locus_tag="VFI43_03550"
     CDS             complement(1818..2621)
                     /locus_tag="VFI43_03550"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET7061235.1"
                     /translation="MIKPLAGISILVTRPPHQAEALSEKIQAAGGNVVLFPVLEILDI
                     PDRRPLLELIGRLHEFDLAIFISPNAVQRAMTLIRANREIPPGLKFAAVGQGTVAVLR
                     KFGLDDVVVPGARFDSEALLELAELKAIAGKRVVIFRGEGGRELLGRTLRERGAKVEY
                     AACYRRVKPDSDIEPVLGALTKGDLKAIIMTSSEGMRNFLEMLGEPAHEQLKKIPMFV
                     SHKRIAENAGKLGCANVVLTPPGDDGLLEGLSKYFRPGFVGRKSLSVRT"
     gene            complement(2618..3583)
                     /gene="hemC"
                     /locus_tag="VFI43_03555"
     CDS             complement(2618..3583)
                     /gene="hemC"
                     /locus_tag="VFI43_03555"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET7061236.1"
                     /translation="MPHLSMPSKIVIATRESALALWQANFIRDRLHELYPQTEFSIFG
                     MTTRGDQILDKSLSKIGGKGLFIKELEQALENRSADIAVHSMKDVPMDMPAGYVLAAI
                     AEREDPRDAFVSIRFPALDALPAGSVVGTSSLRRESQLRARFPHLKVHPLRGNVQTRL
                     RKLDEGQFDAIILAAAGLKRLGLGNRITALLSPEQSLPAVGQGALGIECLADRPDLVE
                     LMQPLHHIPTAQCVEAERAMSRTLGGSCQVPLGGFGQIDGEFLRLRGFVALQDGTRII
                     SDELSGNPERGADIGVHLARRLKEQGAQDILGAMDDKPVEPVSLP"
     gene            3709..6504
                     /gene="ppc"
                     /locus_tag="VFI43_03560"
     CDS             3709..6504
                     /gene="ppc"
                     /locus_tag="VFI43_03560"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7061237.1"
                     /translation="MKTLASVSENFGKTQVDVKDDPLREDIRLLGRMLGDTLREQEGE
                     PTFDLVENIRQTAIRFRRDQDPTARHELEMILNQLSNKATVAVVRAFSQFSQLSNIAE
                     DMHHNRRRRTHLRAGSPPQAGSVALALDRVFASGRGSEELAAFFAKALVSPVLTAHPT
                     EVQRRSILDCQLTIAHLLNERDRMQLTPDELRSNEEGLRAAIQILWQTRMLRSERLSV
                     YDEIKNGLAYYHYTFLAEVPRLYAEIEDLLERRMGSSAPHIPPFLHIGSWIGSDRDGN
                     PFVTHDVMLHAAERQSALALDFYMGEVHRIGRRLSLTERLVDVDDELAVLAEASPDRA
                     PSRADEPYRRALIGIYARLAATSQGLGHAIRQRRPVAPVLPYKESTEFVQELDVVIHS
                     LEQHKFRLLARGELRHLRRAAEVFGFHLAPLDMRQHSSIHEQVVTELLDYGAQKRYSE
                     LSEAERVQHLLAEITTPRLLRSPYLDYSELAQKELSILEAAAEIQKRFGCAALPNYVI
                     SKTDGLSDMLEVALLLKEVGLLRPGTPPQLDVNIVPLFETIADLRGCGQIMHELFSIP
                     YYRMLLESRGNVQEVMLGYSDSNKDGGFLAANWELYKAETELTKVFARHNVELRLFHG
                     RGGTVGRGGGPSYQAILAQPPGSVNGQIRITEQGEVIGSKYSDPEIGRRNLETLVAAT
                     IEATLLCHETLGARGDDYYEVMEVLARDALAEYRNLVYGTPGFNRYFRESTPIQEIAG
                     LNIGSRPPSRKQSDLIEDLRAIPWVFSWSVNRTMIPGWYGFGTAVEAFVQREGRGEKG
                     LALLQEMHRTWPFMQTLLSNMDMVLAKTDLGIASRYAELVSDTKLRDEIFGRIQAEWD
                     RSVKWLFAVTGRTELLQDNPTLARSIRNRTPYIDPLNHLQVELLRRYRSGDATDAVKR
                     AIQLTINGIAAGLRNSG"
     gene            6583..7932
                     /gene="miaB"
                     /locus_tag="VFI43_03565"
     CDS             6583..7932
                     /gene="miaB"
                     /locus_tag="VFI43_03565"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET7061238.1"
                     /translation="MVKKLYIKTFGCQMNEYDSEKMADVLRVAQQMEKTDNPAEADVI
                     LFNTCSVREKAQEKVFHDLGRVRHLKKNNPDLLIGVGGCVASQEGGEIVRRAPYVDLV
                     FGPQTLHRLPQMISARQSTGRPQVDISFPEIEKFDHLPPARVEGAIAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDDILEEIAGLASQGVKEVTLLGQNVNAYRGILSYEGESEI
                     ADLALLLEYIHDIPGIERIRYTTSHPREFTRRLIDSYQKLPKLVSHVHLPVQSGSDRI
                     LAAMKRGYTALEYKSIIRRVRAVRPDISLTSDFIIGFPGERESDFLATMRLIDEVNFD
                     GSFSFIYSPRPGTPAADLQDDTPYEVKLERLQRLQQKLDEQAQAISQSMVGTVQRILV
                     EGISKKSADELCGRTDNNRMVNFPSDPEQIGQSISVKITQALSHSLRGAIEEAVRVE"
     gene            8014..8991
                     /locus_tag="VFI43_03570"
     CDS             8014..8991
                     /locus_tag="VFI43_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7061239.1"
                     /translation="MKSRPVELSLSPADNQRLANLCGVLDENLRQIETVLDVAIARRG
                     DHFTLRGDSARTSLAAEALRSFYAQAQHHLDIEEIQLGLIEIMNPHLRNPVSMEDRPS
                     ESAEPVLLTRRSDLRGRTPHQIEYLRQIQTHDITFSIGPAGTGKTYLAVASAVDALER
                     GIVARILLVRPAVEAGERLGFLPGDMVQKVDPYLRPLYDALYDLMGVDKTGKQFERNA
                     IEVAPLAFMRGRTLNQSFIILDEAQNTTPEQMKMFLTRIGFGSKAVITGDITQIDLAK
                     HQKSGLVEARQVLEKVRGIAFTHFSAEDVVRHPLVQRIVNAYEKYEKQG"
     gene            8966..9523
                     /gene="ybeY"
                     /locus_tag="VFI43_03575"
     CDS             8966..9523
                     /gene="ybeY"
                     /locus_tag="VFI43_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7061240.1"
                     /translation="MKNTKSKDSEAEHPSPSRSGAPQLRLTVQYATHGEGIPTRPMFR
                     KWVKAALTQDAEIVLRVVDEPEGHTLNQSFRGKDYATNVLTFNYGHDYRVDYPAPRPL
                     SGDIVLCAPVIEHEARQQGKNLDAHYAHLTIHGVLHLQGHEHGEDVEAEAMEQLEVEI
                     LGRLGYEDPYRETRTDSSKIHDSLG"
     gene            9594..10430
                     /locus_tag="VFI43_03580"
     CDS             9594..10430
                     /locus_tag="VFI43_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET7061241.1"
                     /translation="MDDPPKPGWLERLSALLLREPGDREQLLALLRCAYERNLFDSDA
                     LNMIEGAIQVSEMQARDIMIPRSQMDVIDISDPPEKFIPFVIEKAHSRFPVTENDKNN
                     VIGILLAKDLLRYHTGEEFSVRDTLRPVVFIPEAKRLNVLLKDFRTNRNHIAIVVDEY
                     GAVAGLLTIEDVLEQIVGDIEDEHDFDEAEDNIVQDSDGHYRVKAITEIEDFNERLGA
                     DFSDTEYDTVGGLVLHKFGRLPKRGESVTEGQFKFTVLRADSRRLYTLLVEKVPLPVL
                     QQ"
     gene            10481..10975
                     /locus_tag="VFI43_03585"
     CDS             10481..10975
                     /locus_tag="VFI43_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7061242.1"
                     /translation="MKSAPVDSAGEAPEVEVIVEIPKGCFLKRGSTGRIDFVSPLPCP
                     YNYGSIPDYLGLEGDLLDAVVLGPRLPLGTKVRTRAWGAVILMDRGMADDKLICSNRF
                     PNSRQRAEIIRFFSFYAKCKGMLNIWRHRPGRNACEGWCEASEALARARPRPTARREP
                     VVGF"
     gene            11143..>11997
                     /locus_tag="VFI43_03590"
     CDS             11143..>11997
                     /locus_tag="VFI43_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7061243.1"
                     /translation="MPLLVLEKTSLAFGHHALLDQVDLQLDPGERVGLIGRNGGGKSS
                     LLRILAGELEPDDGKVRRAPSLKLGYVPQEPGLDASLTVFEEVAKGLGAVSQFLVEYH
                     EASRLLAEQEDDIEVLLSHLQNLQAELEARDGWRTQARVETAIGKLGLSPDDLVGNLS
                     GGQKKRVALARALVVSPDLLLLDEPTNHLDFSSIEWLEGFLKTFPGAILFITHDRRFL
                     NNIATRIIELDRGKLASFPGNFAEYQQKKTEMLEIEAIHYQKFDKVLAQEEVWIRKGV
                     QARCTRNEG"
BASE COUNT         2699 a         3338 c         3334 g         2626 t
ORIGIN      
        1 tggcctcggc gtcctgcatg ttttccatat tttcgagatg ggcgtgacgg cgtaagcgac
       61 tcaatatggg cttgtccact gcgttatgct gttccagttg cccgagcaga ttgagcacct
      121 ccttccagtt cccgagctgc tgctggactt ttatttccag ccatagggca gcctcaggca
      181 tccgtttttc gactgagtgc agaatctgca atacgtgcag ggcttcctcg gagcggccct
      241 catcaaggag aagttctgct ttggtcatca gccgcaaggc tgtttctttc ggcgcgtccg
      301 cttccgccaa tgcaataaat tcatcccgct ccaaatacct gccctgctca tgcgctgccc
      361 gtgccgccac tacggcgttg agagcattca caacaggaga ttccttcatt tccagtgctg
      421 ccgcagatgc tttctcggcc ttgtcgtagc gcctttccag gagcgccttg aaaccttcca
      481 gcatcgcttt atgcgccttc tcgcgacggc ggcgcagccg gaactcacct acttctgctg
      541 gcaaccgtaa agtcatggca gccagtctga caaaaaaata gaggacaaaa aaggccagga
      601 gtaacagaag caccatgagg ttgagcgaga gttcaatccg gtagggttgc gcaacaatca
      661 gaatgtaccc attgtttttc gctgccagag tcgcagctac cgccaccgca aacaacacca
      721 gcagccagaa cgcgggcttc atctgtttcc ccggtctcgc cccaaccggt aatttcgaac
      781 tgcatccagg ctcgcagaaa tgcgaggcaa ttcgaccccc acttcattcc cccgtaattc
      841 ccgtaacatt tcaaggacgc gtattacagg cgcagattgt gtatcgtagt accgctttat
      901 ccagtccgac gcggtgtcga tatccgtatt gaaactggtt ccgtcccttg ccagaagcgc
      961 gttcctcgcg gataacagcc gcagtttcag gttctctcgc aggaaataag cctgcgatgg
     1021 cgacagcaat gcaacatccg gcttatccat gttctggata cgcaccagtt gcttgaggtc
     1081 ttcccaagtc tggcgcacga atcttagcca cgggttttcg gccggcggct gttcccggga
     1141 aacagggggc tctggcggac gaagctccat tgcgaggggt aatttctcga ccgcggccgc
     1201 caggttgtcg aggcggatgc tgatgccaac agtgtcggtg taaggcgcgg cgcgcaggag
     1261 gtccatgtcc gcggcgagca ctttacgtag tggagtgagc tgaggccggc cgatccgctg
     1321 gagcctcgca tccgcttcct gcattgcaat caaggccgct ttgacgttac ttgccaactg
     1381 gagctgctgg ttggcaatga gcagtaactg ctctacttct tcgagcgttg tctcatcccg
     1441 gttccgggta agctcctggt ataattcttc cagagcgagt tgctggctct gggattcggc
     1501 cagccttgcc tcaagcaacc caacctttgt ttccgtccgc cgccctgcct ctaccgcccc
     1561 tttcgcaagc gcccgggatt ccttgccgga cgcatcggct tcggccagcc tttttgccag
     1621 gtcgtgtttt acttccgaaa tctgatcatg agcctcatac cattgccatg cgaggaggaa
     1681 ggcgaaaaaa acagcaagga accagacagg cgtcgcacgg gcccgtagtg ttttccatac
     1741 gccatcactc gccgccttcc tgccttccct gctttcctcc ggatcgggat tggtctcgac
     1801 cggttcggct tgggaactca tgttctcact gaaagcgatt ttcggccgac gaaacccggc
     1861 cggaaatatt ttgacaaacc ttcaagaagg ccatcatccc cagggggggt gaggacgaca
     1921 ttcgcgcaac cgagttttcc ggcattttcg gcgatgcgct tatgtgaaac gaacatgggt
     1981 atcttcttga gctgctcatg cgccggctcc ccaagcattt cgagaaagtt tcgcatgcct
     2041 tcgctgctcg tcatgatgat cgctttcaga tcgcctttgg tcaaggctcc aagaacgggt
     2101 tcaatatcgc tgtctggttt gacgcgacga taacatgcgg cgtactccac cttcgcccct
     2161 cgctccctca gcgttcgccc cagaagctcg cgcccgccct ctccacggaa aatgaccacc
     2221 cgctttccgg cgatcgcctt cagctccgca agctcaagca aggcttcgct gtcgaagcgc
     2281 gccccaggta cgacgacgtc atccaggcca aactttcgaa gcacagcaac cgtaccctga
     2341 cctaccgcag caaacttgag tcccggaggt atttcgcggt ttgcccgtat cagggtcatc
     2401 gcgcgctgaa ccgcatttgg gctgatgaaa attgccagat caaactcgtg aagacggcct
     2461 atcaactcga gcaagggtcg acggtctggt atatccagga tttcaagcac gggaaacaag
     2521 acaacattcc cacccgccgc ctgtatcttt tcggaaagag cctccgcttg gtgtggcgga
     2581 cgtgtgacca ggatggaaat cccggccaac ggtttgatca cggaaggctc accggttcga
     2641 ccggtttgtc gtccatggcc cccagtatgt cctgcgcacc ctgctccttc aaccgcctgg
     2701 ccaagtggac accaatgtct gcgccgcgct ccggattgcc gctcagttcg tcactgatga
     2761 tacgcgtgcc gtcctggaga gcaacaaagc cccgtagccg caaaaactcc ccatcgatct
     2821 gtccgaaccc gcctagcggt acctggcaac ttccacccaa ggtccggctc atcgcccgtt
     2881 cggcttccac acactgggca gtggggatat gatgcagcgg ctgcatgagt tccacaagat
     2941 caggtcggtc agcaaggcat tcaatcccca aggcgccctg acccaccgct ggcaagctct
     3001 gttcaggact cagcaaagca gtgatacggt tacccaaccc caaccgtttc agtccagcgg
     3061 ccgccagaat gatggcgtca aactggccct catccagttt ccgcaaacgg gtctgcacgt
     3121 tgccgcgcag gggatgcact ttcaggtgcg gaaagcgggc gcgcagctgg ctctcgcgcc
     3181 tgaggctgga tgtacccacg acacttccgg cgggaagggc gtcgagcgca ggaaagcgta
     3241 tggaaacgaa agcgtcgcgc ggatcctcac gttcggcaat ggctgccagc acatatccgg
     3301 caggcatatc catcggcacg tccttcattg aatggacagc aatgtcggcg ctgcgattct
     3361 ccagcgcctg ctcgagttcc ttgatgaata atcccttgcc gccgattttg gaaagggatt
     3421 tgtcaagaat ctgatcccct cgcgtagtca tgccgaatat gctgaattcg gtttgcgggt
     3481 ataattcgtg cagccggtcc cggatgaagt tcgcctgcca cagcgccaag gcgctttccc
     3541 gagtagcgat aacaattttt gaaggcatcg atagatgagg cataattact ttgaaaaaat
     3601 cggggaattg ccgccaccac tcattttagc acgcctgatg tcagccatga ccggggaagg
     3661 cccggttcgg aaattggcat cgaacgggct tggaaaaaca aacttgtcat gaaaacgctt
     3721 gcttccgtga gcgagaattt cgggaaaacc caggtggacg tgaaggatga tcctttacgc
     3781 gaagatatcc gcctgctcgg gcggatgctc ggcgacacgc tgcgcgaaca ggaaggcgaa
     3841 cctacgttcg atctggtgga gaacatacgt cagaccgcta tccggttccg gcgcgatcag
     3901 gatccgacgg cccggcacga actggagatg atcctgaatc aactcagcaa caaggcgacg
     3961 gtagcggtcg tgcgcgcatt cagccaattt tcgcagcttt ccaacattgc cgaggatatg
     4021 catcataacc ggcgccgtcg cacccatctt cgagcaggct ccccacctca ggcaggcagt
     4081 gtggcactcg ctctggatcg ggttttcgca agtggaaggg gtagcgagga acttgctgca
     4141 tttttcgcga aagcccttgt gtctccggtt ctgacagctc acccgaccga agtccagcgc
     4201 cgaagcatcc tggattgcca gcttaccatt gcccatctgc tgaacgagcg cgaccgcatg
     4261 cagctcacgc cagacgagct gcgcagcaat gaagaaggac tgcgcgccgc gatccagata
     4321 ttgtggcaaa cccgcatgct gcgatcggaa cgcctgtcgg tatacgacga gatcaaaaac
     4381 gggctggcct attatcatta tacctttctc gccgaagtgc cgcgccttta tgccgaaatt
     4441 gaagatttgc tcgaacggcg gatgggctcc agcgctccgc atattcctcc atttcttcac
     4501 attggcagct ggataggcag tgaccgggac ggcaacccct tcgtcacgca tgacgtcatg
     4561 ctccatgcgg cggagcggca gtcagccctt gcgcttgatt tttacatggg ggaggtgcat
     4621 cggatcggcc gcaggctcag cctgacggag cgcttggtgg atgtcgatga cgaattggcg
     4681 gttttggccg aggcatcgcc cgaccgtgct ccaagccgtg cggatgaacc ttaccgccgc
     4741 gctctcatcg gtatatatgc acggcttgca gcgaccagcc agggcctcgg ccacgccatc
     4801 aggcaaagac ggccggttgc gcccgtcctg ccatataagg aaagtactga attcgttcag
     4861 gaactcgatg ttgtcatcca ttcactggag caacacaagt tcaggttgct ggcgagagga
     4921 gagttgcgtc acctgcggcg ggcggcggag gtgtttggat tccacttggc gcccctcgat
     4981 atgcgccaac atagcagcat tcatgagcag gtggtaacgg aactgcttga ttatggggcc
     5041 cagaaacgtt attcggagct aagcgaagcc gagcgtgtac aacatcttct tgccgagatt
     5101 acgaccccgc gtttgctgcg atctccctac ctggactatt cggagcttgc gcaaaaggag
     5161 ctgagcatcc tcgaggcggc ggccgagatt cagaaacgtt tcgggtgcgc tgcactcccg
     5221 aattatgtca tttccaaaac tgatggcctg tcggatatgc ttgaggtcgc cttgctgtta
     5281 aaggaggtcg gactgctaag gcccggaacg ccgccccagc tggatgtaaa tattgttccg
     5341 ctattcgaaa cgattgcgga tttacgcggt tgcgggcaaa tcatgcatga gcttttttcc
     5401 attccttatt acaggatgct cctcgaatcg cgcggtaacg tccaggaggt gatgcttgga
     5461 tattccgaca gcaacaagga tggtggcttc cttgccgcaa actgggagct ttacaaagcg
     5521 gaaaccgagc tcaccaaagt ttttgccaga cacaatgtcg agttgcgcct gttccacggt
     5581 cgcggcggca ccgtaggcag gggaggcggt cccagctatc aggcgatact cgctcagcca
     5641 cccggcagtg tgaatggtca gatccgtatc acggagcagg gtgaagtgat cggcagcaaa
     5701 tattcagatc ccgaaatcgg gcgccgcaat ctcgaaaccc tggttgccgc caccatagaa
     5761 gccaccctgc tgtgtcatga gacgctgggg gcgcgcggcg acgattatta tgaggtcatg
     5821 gaggtcctgg cgcgggacgc gcttgccgag taccgcaacc tggtttatgg gacgcccggg
     5881 tttaaccgct acttccgtga gtctacccct atccaggaaa ttgccggtct taatatcggc
     5941 agccgccctc catcgcgcaa gcaatccgat cttatagaag atctgcgcgc gattccctgg
     6001 gttttcagct ggagtgtcaa ccgcaccatg atccctggct ggtatggctt tggcacagcg
     6061 gtcgaagcat tcgtccagag agaggggcgc ggggaaaagg gccttgcgct cctccaggaa
     6121 atgcaccgca catggccgtt catgcagaca ttgctttcga acatggatat ggtgcttgcc
     6181 aagactgatc tgggaattgc gtcacgctat gccgaacttg tttcggatac gaagttgcgc
     6241 gacgaaatct tcgggcgcat acaggcggag tgggatcgca gtgtcaagtg gctgttcgcg
     6301 gtcaccggcc gtaccgaatt gctacaggat aaccccacgc tcgcgagaag tattcgcaac
     6361 cgcactccct acatcgaccc gcttaaccac ctccaggtgg aacttctgcg gcgatatcgc
     6421 tcgggcgatg cgaccgatgc agtaaagcgg gcgatccagc tcaccatcaa cggcattgcg
     6481 gctgggctga gaaatagcgg gtaaaagtgg cgcaataacg actgatccag ggtcatgcgt
     6541 tactgtcgag gccgggtatt caatttcaaa ctgggtaaac tcttggtcaa gaaactctat
     6601 atcaagactt ttggctgtca aatgaacgag tacgactcgg agaagatggc cgacgttctt
     6661 cgcgtcgcgc agcagatgga aaagacggac aatcccgcgg aagctgatgt cattcttttc
     6721 aatacttgtt cagtaaggga aaaagcgcag gaaaaggtgt ttcacgacct ggggcgtgtc
     6781 aggcatctga aaaagaacaa tcccgatctc ctcattggcg tcggaggatg tgtcgcgagc
     6841 caggaaggcg gggagattgt gaggcgcgct ccctatgtgg acctggtgtt cggcccgcaa
     6901 accctgcacc gcctgccgca aatgatctcc gcgcgccaaa gcacgggacg tccccaagtg
     6961 gatatttctt tccccgagat cgagaaattc gatcatttgc cccctgcccg ggttgaaggg
     7021 gcaatcgctt ttgtatcaat aatggaaggc tgcagcaaat attgcagttt ttgcgttgtt
     7081 ccatatacga ggggggaaga aatttctcgt cccttcgacg atatactgga ggaaatcgca
     7141 gggctggcca gccagggggt caaggaagtg acattgctcg gccagaacgt gaatgcctat
     7201 cgcggcatcc tgagctacga aggagagagc gaaatcgctg atctggccct gttgctcgaa
     7261 tatatccacg atattccggg tatcgagcgc atccgctata ccacctctca tccgagggaa
     7321 ttcacccggc gcctgatcga ttcctaccag aagttaccga aattggttag ccacgtgcac
     7381 ctgccggttc agtccggatc tgaccgcata ctggccgcca tgaaacgcgg ctacaccgca
     7441 ttggagtata agtccatcat ccgccgcgtc cgcgcggtac ggcctgatat ttcccttact
     7501 tcggacttca tcatcggatt tccaggcgaa cgcgaaagtg attttttggc gaccatgcga
     7561 ttgatcgatg aagtgaattt cgacggctcc ttcagtttca tttatagccc gcggcccggc
     7621 acgcccgctg cggacttgca ggatgacacg ccttatgagg tgaaactcga acggctgcag
     7681 cgcttgcagc aaaagctcga tgaacaggct caggcgatca gccagagcat ggtcggcacg
     7741 gttcagcgca tcctggtgga aggcatatcc aaaaaaagcg cggatgagct ttgcggacgc
     7801 acggacaata accgtatggt taatttcccc agcgatccgg aacaaatcgg acagtcgata
     7861 agcgtaaaaa tcactcaggc tttatctcac tcattgaggg gagcaatcga ggaagcagtg
     7921 cgagtcgaat agtacggaaa ctatcttctt ttcttgcaaa gagggtttgt cgctgccaga
     7981 attaagacat cggacccatc gcttgccctg cctttgaaat cccgccccgt cgaactatcc
     8041 ctttctccag ccgataacca gcgtcttgcc aacctctgcg gagtgctgga tgaaaacctc
     8101 aggcagatcg aaacggtact ggatgtcgcc attgcgcgcc gcggcgatca cttcaccctt
     8161 cggggcgatt cagcgcggac ttcccttgca gcagaagcac tgcgaagctt ttatgcccag
     8221 gcgcagcatc atctggatat tgaagagatt cagctggggt tgatcgagat catgaacccg
     8281 catttacgca accctgtttc catggaagac cgaccgagcg aaagtgcaga gccggtactg
     8341 ctcactcgcc gcagcgattt gcgcggaaga actccccacc agatcgagta tttgcgtcaa
     8401 atccagaccc atgacattac gttttcaatt ggtccggccg gcaccgggaa aacttacctt
     8461 gcggtcgcga gcgcggtgga tgcgctggaa cgggggattg ttgcccgcat cctgctggtg
     8521 cgtccagcgg tcgaggctgg cgagcggctc gggtttttac caggagacat ggtccaaaaa
     8581 gtagaccctt acctgcgccc tctctacgac gcgctttacg atctgatggg cgttgataaa
     8641 accggcaagc agtttgagcg caacgcaatc gaggttgcac ctctcgcctt catgcgcggc
     8701 cgcacgttga accagtcctt catcatactc gacgaggcgc aaaacacgac tcccgaacag
     8761 atgaaaatgt ttttgacccg catcggcttt ggatcgaaag cagtcataac aggcgacata
     8821 acccagattg atctggcgaa gcaccagaaa agtggacttg ttgaggccag gcaagttctg
     8881 gaaaaagtgc ggggaattgc cttcacccat tttagtgcag aagatgtcgt gcgccacccg
     8941 ctggtacaac ggatcgtgaa tgcatatgaa aaatacgaaa agcaaggata gcgaagcaga
     9001 gcatccttcc ccgagccgaa gtggagctcc gcagctcaga ttgactgtcc aatatgcgac
     9061 ccatggagaa ggtattccca cacggccaat gttccggaaa tgggtgaaag ccgcactgac
     9121 gcaagatgcc gaaattgtat tgcgcgtcgt cgatgaaccg gaaggtcaca ccctgaacca
     9181 gagctttcgc ggcaaggatt atgcgaccaa cgtgctgacc ttcaattatg gccatgatta
     9241 ccgggttgat taccctgctc ctcggccttt atcaggggac atcgtgctct gtgcgcccgt
     9301 tatagaacat gaggcgcggc agcaaggaaa aaacctggac gcgcattacg cccaccttac
     9361 catacacggg gtcttgcacc ttcaggggca tgaacatgga gaagacgtcg aggcggaggc
     9421 aatggaacaa ctggaagtgg aaattttagg cagacttggg tacgaggacc cctatcgcga
     9481 aacccgaaca gactcgtcga aaatacatga cagtctcggg tgatgagact tctgtctcct
     9541 tgccgacggt agggcatgga aaaaggacga aaaagaggaa agatctaacc aatatggatg
     9601 atccgcctaa accgggctgg ctcgagcgcc tgagtgcttt gcttttgcgc gaacccggag
     9661 atcgggagca actgcttgcc ttgctacgct gcgcgtacga gcggaatctc ttcgattctg
     9721 acgcgctcaa catgattgaa ggcgcgattc aggtttcgga aatgcaagca cgcgacatca
     9781 tgattccacg ctcacaaatg gatgtgatcg acatcagcga ccctccggaa aaatttattc
     9841 cttttgttat cgaaaaggcg cactcgcgct ttcctgtaac cgaaaacgat aaaaacaacg
     9901 tgattgggat cctgcttgca aaagaccttt tacgctacca tacaggagaa gaattcagcg
     9961 taagggatac gcttcgtccc gtggtattca tcccggaagc gaagcggctc aacgtattgc
    10021 tcaaggattt ccgaaccaac cgtaatcata ttgcaatcgt agtggatgag tatggggccg
    10081 ttgccggcct gctgactatc gaggacgtgc tggagcagat cgtgggcgac atcgaggatg
    10141 agcacgattt cgatgaggca gaggataata tcgtacagga ttcggatggt cactaccgtg
    10201 tcaaggcgat taccgaaatt gaagatttca atgaacggct cggggctgat ttcagcgata
    10261 ccgaatacga cacggtgggc ggcctggtac tgcacaagtt cggcaggctg cccaagagag
    10321 gagaaagcgt taccgagggc cagttcaagt tcactgtgct gcgcgcggat agccgcaggc
    10381 tatataccct gctggtggaa aaggttccat taccagtcct gcagcaatag gaatcctcag
    10441 gtcggatggc atttcttctg ggggagagga ttttgagaac atgaaatctg cgccggttga
    10501 ttccgcaggt gaggcgccag aagtggaggt aatcgtcgag attccgaagg gctgctttct
    10561 caagcgcggt tcgacaggca ggattgattt cgtttcgccc ctgccatgtc cctataatta
    10621 cggctctatt ccggactatc ttggccttga gggcgacctg ctggatgcag tggtactcgg
    10681 tccccgcctg cccttgggga cgaaggttcg cacccgggcg tggggagcgg tgatcttgat
    10741 ggatcgtggt atggcagacg ataagcttat ctgcagtaac cgttttccca attcccggca
    10801 acgcgcggag ataatccgct ttttcagctt ttatgcgaaa tgcaagggaa tgctgaatat
    10861 ctggcgacat aggccgggac gcaacgcctg cgaaggatgg tgtgaggcgt ccgaggcgct
    10921 tgcccgggcc cggcccaggc ctacggcgcg gcgagagccc gtcgttgggt tctaggccct
    10981 attctgtcgt tcaggctcgg taggctgaca gaacttcttt aacgcccccc ttgtctgtga
    11041 gtagccagtc ccattattcc ttgggctatt caagaattcc tttgccccaa ccggtttctg
    11101 gtggcagaat cgcggccttt tcaaactcca ttcgctatta ctatgcctct cctcgttctt
    11161 gaaaaaacat ccctcgcatt tggacaccat gcgttgctgg atcaggtgga tctgcaactt
    11221 gatccgggcg agcgggtggg gctgatcggc aggaacgggg gcgggaaatc aagcttgctt
    11281 cgaattctcg ccggtgaact ggagccggat gacggaaagg tccgtcgcgc accttcattg
    11341 aaactgggtt acgtacctca ggaacctgga ctggacgcct ccctgacggt gtttgaagaa
    11401 gtggcgaaag gcctcggtgc agtaagtcag tttcttgtcg agtaccacga agcgtctcgc
    11461 cttctggccg agcaggaaga cgatatcgaa gtccttcttt cccatcttca aaatttgcag
    11521 gcagagctgg aagcccggga cggatggcgc acccaggcga gggtcgagac cgccattgga
    11581 aagctcggct tgtcccctga cgatctggtc ggaaatctgt caggtggcca aaaaaaacgc
    11641 gtggcgctcg cccgtgcgct tgtcgtttcg cccgacctgc tgttattgga cgaacctacc
    11701 aatcacctcg atttttcttc gattgaatgg ctggaaggct ttctcaaaac attccccgga
    11761 gcaatactgt tcatcaccca tgaccgccgc ttcctgaata atattgcgac gcggatcatc
    11821 gagctcgacc gtggcaaact cgcaagtttt cccgggaatt ttgcggagta ccagcaaaag
    11881 aaaacggaaa tgctcgagat tgaagcgatt cattatcaaa aattcgataa ggtgctggcg
    11941 caggaggagg tatggatacg caagggcgtc caggcgcgct gcacccggaa cgagggc
//
LOCUS       DASSHJ010000089        14430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_626627, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000089 DASSHJ010000000
VERSION     DASSHJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626627"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..1847
                     /locus_tag="VFI43_03595"
     CDS             495..1847
                     /locus_tag="VFI43_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HET7061244.1"
                     /translation="MIERFSIDTFTRNLSGAMWIFARLPDDGTAAALIHRSIQREVWR
                     ELKFGDRVAMCLALPFIPLVFALLVVVFTVANGPAIKKRTGKGIIQQIREQAECAMHL
                     AILPPWYYIFELHDDDKRLHASEYLNRFETKAGLYRFLRDSNGGLPVPEERSTACIKD
                     KARFRDRCREFGVMTAPVLLNVSGEHIGVVDWTSPELPERDLFVKPLHGQGGKKAARW
                     NYLGSGRYKRNDGETLTRDQLLEHLQQESRHTAFLVQPLLVNHKELIDLANGTLATVR
                     VMTCRNEQGEIEVTNAVFRMAQNKSSSVDNFHAGGVAANVDIQTGELGAGTRGAWGVT
                     ADGWYERHSETGAQILHRKLPCWPDLIALVQRAHASAFSDQVVIGWDVAPLDSGPCIM
                     EANKAPDLDIIQRAGGGPIGNQRLGKLLAFNLRRAIEAKHTLPMAPDDAQDLESGRAL
                     "
     gene            complement(1885..2694)
                     /locus_tag="VFI43_03600"
     CDS             complement(1885..2694)
                     /locus_tag="VFI43_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HET7061245.1"
                     /translation="MTGSNPGTPVRAIILSAGQGRRLLPLTQNTPKCLLPVLGKPIIE
                     WQIDALLANGISEITVVTGFQSTLVETLLEQRYADRAQITSLFNPFFEVADNLASCWI
                     ARFMMDHDFVLLNGDTVFEEAVLAKVLSSELAPITLSTDHKLIYDADDMKVQLAETGW
                     VKHVNKTMPADQIDAESVGLTFFRGQGPQEFRSAIETALRNQAELKSWYLTIIDALAG
                     KQIVNGCSVSGHLWCEIDFGADLARAEAVFANCPSGMDLRILPSTEIVVHQ"
     gene            complement(2860..3501)
                     /locus_tag="VFI43_03605"
     CDS             complement(2860..3501)
                     /locus_tag="VFI43_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7061246.1"
                     /translation="MKTVHWEAIIFDFDGVVVESGDIKTQAFVNLYREYGNEVMAEVA
                     EYHCLNGGMSRYHKFRYFQQHLLKKPPLTAEEENILDRRFSELVVEAVIASEGVPGAA
                     ELIRRESARIPLFIASGTPETELNVIVKRRGLAHFFAAVRGSPAPKQTLIADILAAHE
                     LPPERVLMIGDALVDYKSAQLNKVSFLGRVRPGDQNPFPPDVTTLPDLSGLLA"
     gene            complement(3498..4430)
                     /locus_tag="VFI43_03610"
     CDS             complement(3498..4430)
                     /locus_tag="VFI43_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7061247.1"
                     /translation="MTTNEIAVVYGASGFLGSHVADALSNAGYRVRLFDCRSSPHLRP
                     DQEMIIGDIMDLRQVIDAARGAHVVYNFAAIADIDEAHDKPLATATVNVLGNMHALEA
                     ARIASARRFVFASTIYVYSESGSFYRASKQAAERFIETYHERYGLDYSILRYGSLYGR
                     RADARNGIYRMLHEALSRHSITYRGTGEAIREYIHVEDAARMSVQVLAPEFANRHLIL
                     TGQERLRIKDVMTMISEIMPWEVELHFEKADAVHHYEVTPYAFQPRIGHKLVLNEHVD
                     LGQGLLDCLRDIHQAMHHAGGDNELVPLASDNRA"
     gene            complement(4432..5373)
                     /locus_tag="VFI43_03615"
     CDS             complement(4432..5373)
                     /locus_tag="VFI43_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7061248.1"
                     /translation="MTRLVISTSSFDLDSNPPVQRLIRAGMQIAQNSHGRKLTEDEAT
                     ALIGADTVGMIAGIEPLTERVLASAKSLKVISRCGTGIDNVDLDAARRYGITVLNTPE
                     APAQAVAELTLGLMLAVLRQIRQTDRRLRDGEWPRTQGRLLAAQCIGIVGLGRVGRRV
                     AQACQALGAQVLAHDSYVTEAPPGVRLIPLDELLGKADIVSLHLTYDAHTHHLINREA
                     LARMKRGAVLINTARGGLIDESALLDALTSGQLEGAALDVFEQEPYRGPLLTCENTVL
                     TSHVGSLAREARHRMEIEAAENLLLGLSNAGILRDEQ"
     gene            complement(5370..6131)
                     /locus_tag="VFI43_03620"
     CDS             complement(5370..6131)
                     /locus_tag="VFI43_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7061249.1"
                     /translation="MELKSKLARNALTIGSWITLGHPSIAEIMAGAGFDWLVLDMEHS
                     VLELSDVQMLIQVLDGRGCPAIVRLTSNDADQIKRVMDAGATGIMVPMVKSAADAQVA
                     VQAVYYPPRGQRGVGLARAQQYGAGFQQYRNWLDNNAVVVVMIEHIDAVEAIDSILSV
                     PGVDAYIIGPYDLSGSMGRPGELDYPEVQTAMERVLDAGRRLGKAGGVHVVEPDPGLL
                     RRNIEAGFSFIGYGLDIRILDSLCRTHMRDIRADI"
     gene            complement(6163..6921)
                     /locus_tag="VFI43_03625"
     CDS             complement(6163..6921)
                     /locus_tag="VFI43_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET7061250.1"
                     /translation="MKTIAVIPARMGSSRFPGKPVAKLLGRTMLEHVYRRVSLSHAVD
                     ATFIATCDEEIREAALAFGANVIMTSDAHERASDRVAEAVADMDAELIVMVQGDEPMT
                     RPEMIDAAVAPFGAEPQLGCVNLVHSIDNRDDFYDFNTIKVVMNQKDEALYMSRQPIP
                     TAPASVQSVFSHHLAYKQVYIIPFRRATLLDFARLPPTPLEKLESIDMLRLIEHGYPV
                     KMVRTKFNTQAVDTPADLERVAWLMESDPLLASY"
     gene            7414..8490
                     /locus_tag="VFI43_03630"
     CDS             7414..8490
                     /locus_tag="VFI43_03630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7061251.1"
                     /translation="MTPAASATSSIDVVIPVYNAPQLTRRCIESVVAWLSPSVRYIHV
                     QDDASEAETREMLDNLPYASLRVHHSLRNQGFGASVNEAVSRSDASLILVLNSDTAVF
                     ENILPALSAALEADPTLALIIPGGNDYARDNLPRYVCRPGGYVRTHRLRGHAFLMRRN
                     VFLEAGGFDPAFGRGYYEDTDLGRRLNLSGWQIGVHPDASIQHEGGGSFGRGQSYMRL
                     VKRNRALYFSRYPHASLNVLLLSGNCPLTHFPSELIDALEYVFREGGYVHWLTPEAPR
                     LLLCLQMRSYCMGVQGIIRLLLRDFREEKRVSEIWMLPDTPRLLHLVARAWARARGIK
                     VKSWSWASTEKCAQSVASTSALVR"
     gene            complement(8527..9597)
                     /gene="corA"
                     /locus_tag="VFI43_03635"
     CDS             complement(8527..9597)
                     /gene="corA"
                     /locus_tag="VFI43_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET7061252.1"
                     /translation="MGSHSRKRSAKAGLAPGTLVRIGKKEHGASHATLLQYDENSLRE
                     SEVGSAELCDKIKPYEGTTWLNLSGLSDVGMMEQIGKCFNLHPLVLEDILNTEQRSKL
                     EDYGDYIYIVLKTFGYENKGDEERLSSDQISLVIGKNFVLSFLETAESQFAAVRERLR
                     SGKGQIRKLGADFLLYSLVDAVVDSYFVILERLDEKTEALETELISRPLPGTLHSIYR
                     LKRQNVFLRRSLWPMREVINSLQRDDSPLVTRNTSLYLRDVYDHTVHIIDSVETLRDL
                     NAGMLDIYLSSVSYRISAVMKVLTVITTIFMPLTLITGIYGMNFKYLPGLEWEGGFFA
                     VLGGMMAISLGMLGLFRWKKWL"
     gene            complement(9603..11165)
                     /gene="ppx"
                     /locus_tag="VFI43_03640"
     CDS             complement(9603..11165)
                     /gene="ppx"
                     /locus_tag="VFI43_03640"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HET7061253.1"
                     /translation="MLAYSTLAAVDLGSNSFRLQIARVVGKQLYPLDSLREMVRLAAG
                     LDANRRLNEESQVRALECLRRFGERLRGLPPHAVRAVGTNTLRVAKNAPAFLKKAEHA
                     LGFPIEVISGHEEARLIYLGVAHSLPASPNNRLVVDIGGGSTELIIGNRFKPLKLESL
                     YMGCVNYSQRFFPDGKITRAAMRRAELSARGELQGVVSEFSSDHWEEAFGSSGTARAL
                     GDIVKLNSFGNDGRDGVITPQGLDAFRSYLLKAGDTKNLEIFGLRDDRAPVIPGGFAI
                     MSAAFSELGIRRMSQGMGALRQGVLYDLLGRFHKHDIRETTVRQFMQRYRVDSAQATR
                     VQTLALLLGKQLTAVFEDADEGLLILSWAARLHEVGISVAHSGYHRHSAYILANADMP
                     GFSKSEQERLSVLTLAHRGTIAKGGKFPTDPVSIALLFSLRLAALICRSRRDLDLPSL
                     EVRWKGPEFILLVEQDWLELNPLTEAALSAEAEQWASLGFQLRIENLPAPDPVTGTNA
                     SSLGKARSATGA"
     gene            complement(11232..11939)
                     /gene="phoU"
                     /locus_tag="VFI43_03645"
     CDS             complement(11232..11939)
                     /gene="phoU"
                     /locus_tag="VFI43_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7061254.1"
                     /translation="MASSEHISKQFDAELEAVRTRVLHMGGFVEEQIEKAIEALTSGN
                     EDLIESIITNDHRVNAMEVSIDEICSQIIARRQPAAGDLRMIMTVIKTITDLERIGDE
                     AEKIARMAKLIYETDRMHMPRFVEIRHVAHIAMEMLRKSLDAFARVDLAAAAQVVRQD
                     ELVDEEFRSIMRQLITFMMEDPRKISTALEILFIAKAIERIGDHAKNMSEYVVYMVKG
                     RDVRHVTIEEIEREIRE"
     gene            complement(12037..12696)
                     /gene="rpiA"
                     /locus_tag="VFI43_03650"
     CDS             complement(12037..12696)
                     /gene="rpiA"
                     /locus_tag="VFI43_03650"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET7061255.1"
                     /translation="MSQDRNKRAAAHAAIQHIPAACVVGVGTGSTANYFIDELSSIKN
                     RINGAVASSQATAQRLAAYGIEVMDLNSVDAIPVYVDGADEITQHLHMIKGGGGALTR
                     EKIVAAVAKKFICIADEGKLVSILGKFPLPVEIIPMARGYVAREITRLGGQPVLREGF
                     VTDNGNAILDVHGLQILNPVELESTLNQITGVVTNGLFARRGADMLLLGTDGGVRTFE
                     Q"
     gene            12833..14344
                     /gene="ilvA"
                     /locus_tag="VFI43_03655"
     CDS             12833..14344
                     /gene="ilvA"
                     /locus_tag="VFI43_03655"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HET7061256.1"
                     /translation="MKNGYLERILTAQVYDVAIESPLELAPNLSSRMGNSLWIKREDV
                     QEVFSFKVRGAYNKMVKLPPEALKRGVITASAGNHAQGVALAAQRLNCRATIVMPVTT
                     PQIKMQAVQARGATVITHGDSYDEAYAQARKLADEQNITFVHPYDDPDVIAGQGTIGM
                     EILRQHRGPVHAIFAPVGGGGLISGIAAYVKRLYPEIKIIGVEPVDADSMYQSLQKNR
                     LVTLPQVGLFADGVAVKQVGVETLRLCRELVDEIILVDTDAICAAIKDIFEDCRAILE
                     PSGALSIAGAKAYAKREHIEGKNLVAIASGANMNFDRLRHISERAELGEHREAVMAVT
                     IPEEPGSFKKFCAMLGAKSITEFNYRYADPKEAHVFVAVSVRDREEAASLIKSLVESG
                     LDTVDLSDNEMAKLHIRHLVGGRAREVKNEMLYRFEFPDRPGALMKFLNSMSHHWNIS
                     LFHYRNHGADYGRVLVGIQVPPEEKAEFRAFLSQLDNHYWDESNNPAYKLFLG"
BASE COUNT         3270 a         3920 c         3894 g         3346 t
ORIGIN      
        1 ctggggacgt aacaacacgg tccagtgatc gtattggcgg attatcgttt gcggagcgcc
       61 aaacttgagc attgttgcgt taaagattga cgcgtttttt tgagaagatg ggatcatccg
      121 aatcgtatgg gcaggttgag gcatctgcga gaaattttat acgaatacga ctattcgtac
      181 tcgggggccg aatgccgatg caggccggtg ggacggctgc gatggtaaca gcttttttcg
      241 gaccagcgcg agcacgcggg aactcagtac cgggagcctg tagtccgaat ggtctatttg
      301 taccctgttt gtaccctatt tataccttgt ggtggctcaa ttattttaat attggtgaca
      361 tattattata tagataggtt tcactaacta gcgtagtatc acccctccat agcggaggag
      421 gccggacggt tcagcatgag ctcgacagcc ttgtttcagt aacattttca taggacacgt
      481 ggagaagccg ggtaatgatc gagagattca gtattgacac atttacccgg aacctgagcg
      541 gcgcgatgtg gatattcgcc agattgccgg acgacggcac cgctgccgct ttgattcacc
      601 gtagtatcca gcgtgaggtt tggcgtgaat taaagttcgg tgatcgcgtg gcaatgtgcc
      661 tcgcgctgcc gtttattccg cttgtatttg cattgctggt agtcgttttc acggttgcca
      721 acggaccagc cataaagaag aggacgggga aggggatcat tcagcagatt cgggagcagg
      781 ccgaatgcgc gatgcatctc gctatactgc cgccatggta ctacatattt gaattgcacg
      841 atgacgacaa gagactgcat gcgagtgagt acctgaaccg gttcgaaacg aaagcgggtc
      901 tctatcgttt tttgcgcgac agcaacggtg gtttgcctgt tccggaggaa cgtagcacag
      961 cttgtataaa ggataaggcg cgctttagag accgctgtcg ggagttcggc gtgatgactg
     1021 ctcccgttct cctcaacgtg tccggtgagc acatcggggt cgtggactgg acgtcgccgg
     1081 aattgcccga gcgcgacctc tttgttaagc cactgcacgg ccagggtgga aaaaaagctg
     1141 cgcgatggaa ttacctgggg tcaggcagat acaagcgaaa tgatggcgaa acgttaacca
     1201 gggatcagtt gctggaacac ttgcagcaag aatcgcgcca tacggcattc ctggttcagc
     1261 ccctcctcgt aaatcacaag gaacttattg atcttgcaaa cggcaccctt gccactgttc
     1321 gtgtaatgac ctgccgcaat gagcaggggg aaattgaagt taccaacgcc gtgtttcgga
     1381 tggctcagaa caagtcatca tccgttgata actttcatgc ggggggtgtt gctgccaatg
     1441 tggacattca aacaggcgaa ttgggcgctg gaacccgggg cgcctgggga gtcaccgcgg
     1501 acggatggta tgaaaggcat agtgagacag gcgcacagat tttacatcga aagctgccgt
     1561 gctggcccga tcttatcgcg ctggtgcagc gcgcgcatgc cagcgctttc tccgaccagg
     1621 tcgttatcgg ttgggatgtt gccccgcttg acagtggtcc ctgcatcatg gaagccaaca
     1681 aggcgcccga tctggacatt attcagaggg caggcggtgg accgattggg aatcagcggt
     1741 tgggcaagct tctggcgttc aacctgaggc gggccatcga ggcaaagcac accctcccta
     1801 tggcgcctga tgatgctcaa gacctcgaat caggcagagc gctgtaggag cgtgtagccg
     1861 ctggccgttc gatttcgggc tctcttattg gtgcacgaca atttcggttg aaggtaatat
     1921 tctgaggtcc ataccgcttg ggcagtttgc gaaaactgcc tcagctcttg ccagatctgc
     1981 tccgaaatca atctcgcacc agagatgacc cgagacagag cacccattaa ctatctgttt
     2041 tccagcaagt gcatcgataa tcgtaagata ccaggacttc agttcagcct ggtttcgtaa
     2101 cgcagtttca atggcagatc gaaactcctg ggggccctgc ccacggaaaa aagtaagccc
     2161 caccgactcc gcatcgatct gatccgcggg catcgtttta ttcacgtgct tgacccatcc
     2221 tgtttcagcc agttgcacct tcatatcgtc cgcatcatag atgagcttat gatcagtgct
     2281 cagagtgatt ggcgcgagtt ctgagcttag cacctttgca agcaccgcct cctcaaaaac
     2341 cgtatcccca ttcagcagca caaaatcatg gtccatcata aaccgagcga tccagcagct
     2401 tgcgaggtta tctgcaactt cgaaaaacgg attaaacaga gacgtgatct gggctcgatc
     2461 cgcgtagcgc tgctccagca gggtttctac caaagtggat tgaaaaccgg taaccactgt
     2521 gatttcgctg atcccgttag cgagcagcgc atcaatctgc cattcgataa taggtttacc
     2581 tagaaccggt aacaggcatt ttggcgtgtt ctgggttaac gggagcagcc ttcgtccttg
     2641 gcctgcactc agaataatcg ctctaacagg tgttcctggg ttgctgcctg tcataatttg
     2701 tatgatttat agaagtaaaa ataaacgaat aaacagcccg tctacagcct tttgaagtcc
     2761 gggcctgaat gttttaagcg cttgcaagaa aggttaaaac ttcaaaccca tcttccgatg
     2821 taattcaggc tgcgatgaaa cacgctcatt ccgatccgat caggcgagca gcccgctcag
     2881 gtcgggtagc gttgttacat ccgggggaaa cgggttctgg tcgcccggcc ggacccggcc
     2941 tagaaatgaa accttattaa gctgcgcact tttataatcg accagcgcgt caccgatcat
     3001 caggacgcgc tcgggcggca gctcatgcgc tgccagaatg tctgctatga gcgtctgttt
     3061 gggggcaggg gagccgcgca cagcagcaaa aaaatgcgcg aggccccggc gtttcacgat
     3121 aacgttgagc tccgtctccg gtgtccctga tgcaatgaac agcggaattc tagctgattc
     3181 tctacggatg agctcggctg cgcctggaac accctcgctg gcgatgacgg cttcgactac
     3241 caactccgag aaacgccgat ccagaatgtt ttcttcttca gccgtcaacg ggggcttttt
     3301 cagcaagtgt tgctggaaat agcggaattt gtgataacgc gacatgccac cgttcaggca
     3361 gtggtattcc gcgacctcag ccattacctc gttcccgtat tcgcggtaaa ggtttacaaa
     3421 tgcttgtgtc ttgatatctc cggactcgac cactaccccg tcgaagtcga agataatcgc
     3481 ctcccagtgt acggtcttca tgctctgtta tccgaagcca gggggacgag ctcattgtct
     3541 ccgcccgcat gatgcattgc ctgatggata tcgcggaggc aatccagcag gccctgtccc
     3601 agatcgacat gctcattcag taccagttta tgcccgatac ggggctggaa agcgtaagga
     3661 gttacctcgt aatgatgcac ggcatcggcc ttctcgaaat gcagttccac ctcccagggc
     3721 attatttcag agatcatggt catgacatcc tttattctca ggcgctcctg gccggtaaga
     3781 atcaaatggc gattggcaaa ctctggcgcc aggacctgaa cactcattcg tgcggcatct
     3841 tccacgtgaa tatactcgcg gatggcttcg cccgtgccgc gataggtgat cgaatgccgt
     3901 gacaatgcct catgcagcat gcgatagata ccgttacgcg catccgcgcg ccttccatac
     3961 agcgagccgt agcgcaaaat gctgtaatca aggccgtagc gctcatgata agtttcgatg
     4021 aaccgttccg ccgcttgctt gcttgcccgg tagaatgagc cggactcgga gtagacatag
     4081 atagtgcttg caaagacgaa gcggcgggcg ctggcaatac gggctgcttc caaggcgtgc
     4141 atattcccca acacgttgac tgtggccgtc gcgagaggtt tgtcgtgcgc ttcgtcaatg
     4201 tctgcgatgg cggcaaagtt gtataccaca tgggcgcctc ttgcggcgtc aatcacctga
     4261 cggaggtcca tgatgtcccc tatgatcatc tcctgatccg gacgcaagtg aggtgacgaa
     4321 cggcaatcaa acaggcgtac ccgatatccc gcgttggata gcgcatccgc cacatggctt
     4381 ccgaggaagc cgctcgcgcc atataccacg gcgatctcgt tcgtcgtcat cctactgctc
     4441 gtccctcagg atccccgcat tgctcaggcc tagcagcaaa ttctcggcgg cttcaatttc
     4501 catacggtgg cgggcctccc gtgcgagcga accgacatgc gaggtgagaa ccgtgttttc
     4561 acaagtcagc aaggggccgc gatagggttc ctgctcgaaa acatcaagcg cagcaccctc
     4621 cagttgccct gaggtcaggg cgtccagaag cgcagattca tcaataaggc ccccgcgcgc
     4681 agtattgatt aacactgcac cgcgtttcat tcgcgccaga gcttcccggt tgatcaagtg
     4741 atgcgtatgt gcatcataag tcagatgcag gcttacgatg tctgcctttc ccaggagttc
     4801 atccagggga attagcctca ctcccggagg ggcttcggtg acataagagt catgggccag
     4861 aacctgcgca cccaaagctt ggcaagcttg cgcgacgcgg cgtccaacac ggcccaggcc
     4921 aacaatgccg atgcattggg cggccaggag gcgaccctgg gttctcggcc attcgccatc
     4981 tcgcagccgt ctgtccgtct gccgtatctg cctcagaaca gcaagcatca atcccagggt
     5041 caattccgct accgcttgag caggagcctc aggcgtgttg agaacagtaa ttccgtagcg
     5101 cctcgccgcg tccagatcga cgttgtcgat gcccgtcccg caccgggata tgactttcaa
     5161 gcttttcgct gacgccagaa cgcgttcggt caggggttct atgcccgcga tcatccccac
     5221 agtatccgct ccgatcagtg ctgtcgcttc atcttccgtt aatttgcgtc cgtgactatt
     5281 ttgcgcaatc tgcatgcctg ctcttatcaa ccgttgaaca ggagggttgc tgtccaggtc
     5341 gaacgatgag gttgagatga caagtctggt catatatctg ctctgatgtc ccgcatatgg
     5401 gttcggcata gtgaatcgag tatacggata tcgagaccgt agccgataaa gctgaagccg
     5461 gcctcgatgt ttcgccgcaa caggcctgga tcaggttcta ctacatgaac tcccccggcc
     5521 ttgcccagac ggcgtccagc gtcaagaacc cgttccatcg ccgtttgaac ctcgggataa
     5581 tcaagttcgc ccggtcgtcc catcgagccg gacaggtcat aaggcccgat aatataggca
     5641 tccactccgg gaacagaaag aatggaatcg atagcttcta ccgcgtcgat atgctcgatc
     5701 attaccacca ctacggcatt attgtccagc cagttccggt attgctggaa accagcgccg
     5761 tactgctgag cgcgcgcaag gccgacaccc cgctggcctc ggggcggata atacacggcc
     5821 tgcacggcaa cctgtgcatc cgcagcggat ttaaccatgg gaaccattat ccctgttgcc
     5881 cccgcgtcca tcacacgctt gatctggtct gcgtcattcg aagtcaatcg gacgatggcg
     5941 gggcatcccc tgccatcgag cacctggatc agcatttgta catcgctgag ttcaaggacg
     6001 ctgtgctcca tatcgagcac gagccagtca aatccggccc cagccatgat ctcggcaata
     6061 gagggatgtc caagagtgat ccatgacccg atcgtcaggg cattacgggc aagttttgat
     6121 tttaattcca ttgaaagcag cggaaaaact ctgcagacgt gtctagtaag aagcaagcag
     6181 cggatcggat tccatcaacc acgcgaccct ttccagatca gcgggagtat cgacggcctg
     6241 agtgttgaat ttcgttctga ccatttttac cggataaccg tgctcgataa gtcgcagcat
     6301 atcgatggac tccagctttt cgaggggggt aggcggtaac cgggcgaagt cgagcagcgt
     6361 tgctcgccga aatgggatga tgtagacctg cttgtaggca agatgatgcg agaaaacgga
     6421 ctggaccgag gctggagcag tgggtatggg ctgccgggac atgtaaagcg cctcgtcctt
     6481 ctgattcatg acgaccttga tggtattgaa gtcatagaaa tcatccctgt tgtcgataga
     6541 atgcacaagg ttgacacagc cgagctgggg ttcggcgcca aatggggcaa cggccgcgtc
     6601 tatcatctca ggacgcgtca tcggctcgtc tccctgcacc atcacgatca actccgcatc
     6661 catatcagcc acagcttccg ccacccggtc gcttgccctt tcgtgggcgt cggaggtcat
     6721 gatgacgttc gcgccaaaag ccaaagctgc ttctcgaatt tcttcatcgc aagtggctat
     6781 gaaagttgca tcaactgcgt ggctcaagga tacgcgcctg tagacatgct ccagcatcgt
     6841 gcgacccagc aatttcgcga ctggcttccc gggaaagcgg gaggagccca tgcgagcggg
     6901 tatgactgca attgttttca tgatgtgatg atctgaccgt cggaagcgct ggcacgcgtg
     6961 atattactga tggatgtata gaggcgctat tcgagcttgc cggatcagaa attttctgac
     7021 agtttcacgg gtattttcca gggacgctat cgtaccacta cagataacat tccgggaggg
     7081 tccagaggca atttgaggta cgtcgaaatg tggaaaagag gaatggactg cgagttcact
     7141 gtggctgtcg acaagcccta catatataat aagaaatttt ttgcacggta aacgggtcta
     7201 aaagacgctg aacggacgcg ataaccgtta tgtcagttac agaacaataa cgggtatcaa
     7261 gctcgttttc tagccgcaga tgcaacccct tatgaaaccg gaggaaaagg ttcggcagcg
     7321 ctcggtcacc gatcaggctc attttgaagg gccatcgcct gattttgtat ttgcgcgact
     7381 aacgctttcg gtacattaaa cgaactgatg tccatgactc ccgccgcgtc cgctacctct
     7441 tctatcgacg tagttattcc agtatataac gctccccagt tgacccggcg ctgcatcgaa
     7501 tcggtagtgg cctggctgag cccgtccgta cggtatattc acgttcagga tgatgcatcg
     7561 gaagccgaga cacgagagat gctagacaat ctcccctacg catctctccg cgtgcatcat
     7621 tccttgcgga accaggggtt tggggcatcg gtaaatgaag cggtcagccg ctctgatgct
     7681 tccctcattc ttgtgctgaa ttcggatacc gcagtttttg aaaacatttt gcctgcgctg
     7741 tcggcggcac tcgaggcgga tcccacgctc gccctgatca ttccgggtgg caatgactat
     7801 gcgcgggata atttgccccg gtatgtttgc aggcccggtg gttacgtccg cacgcaccgt
     7861 cttcgagggc acgcgttcct gatgcgccgt aacgtctttc ttgaggcggg ggggttcgac
     7921 ccggctttcg gacgaggcta ttacgaagac accgaccttg gaaggcggct gaatctgagc
     7981 ggctggcaga ttggcgtgca tcccgacgct agcattcagc acgagggggg tgggtcattt
     8041 ggtcgtggac agtcgtacat gaggctggtg aagcgcaacc gggcgcttta cttctcgcgc
     8101 tatccccatg cttccctcaa cgtactgttg ctatccggca actgcccatt gacgcatttc
     8161 ccttccgagc ttatcgacgc gctggaatac gtattccgcg agggaggata tgtgcattgg
     8221 cttactcccg aagcgccccg attgcttctc tgcctgcaga tgcgtagcta ctgcatgggg
     8281 gtgcagggga tcattcggct gttgctgcgc gattttcggg aggaaaagag agtatccgaa
     8341 atatggatgc ttccggatac tccccgtttg ctgcatcttg ttgctcgcgc ttgggcccgt
     8401 gcgagaggta tcaaagtcaa atcgtggtcg tgggcatcaa cggaaaagtg cgcacagtca
     8461 gttgcttcaa catcagcctt ggtccgttaa gcgctgggga agcacgatgc tctatgcggg
     8521 ttcagcctac agccatttct tccaccggaa cagcccaagc attcccaggc tgatcgccat
     8581 cattcctccc agaacagcaa agaatccgcc ttcccactca agtccaggaa ggtatttgaa
     8641 gttcattcca tagattccgg taatcaatgt cagcggcatg aatatggtgg tgatcaccgt
     8701 aaggactttc attaccgcgc tgatgcgata gcttacgctc gacaggtaaa tgtcgagcat
     8761 tcccgcgttc agatccctca gggtttcgac tgaatcgata atatgaacgg tgtgatcata
     8821 gacatcacgc agatacagag atgtattgcg cgtaacgaga ggcgaatcat ccctttgcag
     8881 cgaattgata acctcgcgca tcggccagag cgacctgcgc aaaaaaacgt tttggcgctt
     8941 caggcggtaa atcgaatgca gggtgcccgg taaaggacgc gatatcaatt ctgtttcaag
     9001 cgcttccgtc ttttcgtcga gccgctccag aataacgaaa tagctatcca ctactgcatc
     9061 gaccaggctg tacagcagga aatccgcgcc cagttttctt atctggcctt ttccggagcg
     9121 cagccgctcg cgcaccgcgg caaactggga ttctgcggtt tcgagaaagg aaaggacaaa
     9181 gttcttacct atgaccaggc tgatctgatc cgaacttaat ctttcctcgt ctcccttgtt
     9241 ttcatagccg aaggtcttca atactatata gatataatcg ccgtagtctt caagcttcga
     9301 gcgttgttca gtgtttaaaa tatcttccag caccagggga tggagattga agcacttgcc
     9361 gatttgctcc atcattccca cgtcgctcaa gccgctcaga ttgagccagg tagttccttc
     9421 atacggtttg atcttgtcgc atagctcggc ggagccgacc tcggattcgc gtaaactgtt
     9481 ttcgtcatat tgaagcaagg ttgcatgaga cgcaccgtgc tcctttttgc cgatcctgac
     9541 caatgttccc ggtgccagtc ctgcttttgc cgatcttttt cttgaatgcg atcccatcgc
     9601 cgctacgctc ccgttgcgct ccttgccttg cccagggaag atgcattcgt tccagtgacc
     9661 ggatcaggag cgggaagatt ctcgatgcgc aactgaaacc cgagagacgc ccattgctcg
     9721 gcttctgcag acaacgcggc ttcagtcaac ggattaagct ccagccagtc ttgctcgacg
     9781 aggaggatga attccggccc tttccagcgt acctccaggc tgggcaagtc cagatctctg
     9841 cgactgcggc aaatgagtgc cgcgagccgc agggaaaaca gcaaagcaat gcttaccggg
     9901 tcggtaggaa atttgccccc cttggcaatc gtaccccgat gggccagggt aagcacgctc
     9961 agacgctcct gctcgctttt cgagaagcca ggcatgtcgg cattcgcgag gatgtatgcg
    10021 gagtgcctgt ggtagcccga gtgcgctacg gagatgccga cttcgtgtaa ccgcgctgcc
    10081 caggaaagaa tcagcagccc ctcgtccgcg tcctcaaaga cggcggtcaa ttgcttcccg
    10141 agcagtaagg cgagcgtttg aacccgggtt gcctgagcag aatccaccct gtagcgctgc
    10201 atgaattgcc tgacagtggt ttcacgaata tcatgtttgt ggaagcgccc cagcaaatca
    10261 tagagaactc cctggcgcaa cgcgcccatg ccttgcgaca tgcgccgaat gccgagttct
    10321 gaaaatgcag cggacatgat tgcgaagccg ccgggaatca cgggcgcgcg atcatcgcgc
    10381 agtccaaaga tttcgaggtt tttagtatcg cctgccttta gcaaatagct acgaaaggca
    10441 tccagcccct ggggagtaat cacgccatca cggccatcgt taccaaagct gttcagcttg
    10501 acaatatcgc cgagcgcgcg cgcagtgccc gatgagccga atgcctcctc ccaatgatca
    10561 ctggagaatt cggagacgac tccttgcagc tcgccacgag cggaaagttc agcacgcctc
    10621 atcgccgcac gcgtgatttt accgtcgggg aaaaagcgct ggctgtagtt gacgcaaccc
    10681 atgtacaagc tctcaagctt gagcggctta aaccgattgc cgattatgag ttcggtggag
    10741 ccccctccga tgtccacaac gagacggtta ttcggggagg caggaagact gtgagcaacc
    10801 ccaagataaa tgagacgtgc ttcctcgtgc ccggagatca cttcgatggg aaaaccgagg
    10861 gcatgctcgg ctttcttcag gaaggcaggc gcgtttttgg cgaccctcag ggtattcgtg
    10921 cctactgctc gtacagcatg gggcgggagc ccacgtaacc gctctccaaa gcgccgcagg
    10981 cattccaggg cgcgcacctg tgattcctca ttcagccgcc ggttcgcgtc caggcctgcg
    11041 gcaagcctta ccatttcccg caagctatca agcggatata gctgtttgcc taccacgcgc
    11101 gcaatctgca gccggaagct gttggaaccg aggtctacag ccgcaagtgt ggaataggct
    11161 aacatgggat tccgattgga aagcgtgtta tcaaccgggc cgctttgagc ggaaattgcg
    11221 gcgagatccg gttactccct tatctctctt tcgatctctt caatagttac atgccgcacg
    11281 tcccggcctt taaccatgta gactacatac tccgacatgt ttttggcgtg gtcgccgata
    11341 cgttcgattg ccttggctat aaaaaggatt tccagcgcag tggatatctt gcgcggatct
    11401 tccatcatga acgtgatgag ctgacgcatg atcgaccgga actcttcatc cacgagctca
    11461 tcctgtctca ctacctgggc agccgccgcc agatcgacgc gggcaaacgc atcaagcgat
    11521 ttacgcagca tttccatcgc gatgtgcgcc acgtgcctga tctcgacaaa ccgcggcata
    11581 tgcatgcgat cagtttcata gatcagcttc gccatacgcg cgattttttc cgcttcatcg
    11641 ccaatgcgct cgagatcggt gattgtcttt atcaccgtca tgatcatgcg cagatcgcct
    11701 gcggcaggtt gccgacgcgc gatgatctga ctgcagatct catctatcga tacttccatc
    11761 gcattaacgc gatgatcgtt cgtaatgatg ctttcgatca ggtcctcgtt cccgcttgta
    11821 agcgcttcga ttgctttttc gatctgctct tccacgaacc cgcccatatg cagcacgcgc
    11881 gtgcgtacag cctcgagctc ggcatcaaac tgttttgaaa tgtgttcgct acttgccatt
    11941 gctcacctgt ccatttgact caaagcagga attattcaga ggttcccagc tgtcattgca
    12001 gtttcatcgc cgttcgcgca accgctaccg tcaaggctac tgttcgaacg tacgtacccc
    12061 gccatcggtt cccagaagca acatatctgc cccgcgtcgc gcgaaaagcc cattggtaac
    12121 caccccggtt atctgattca gcgtgctttc gagctcgacg gggttcagta tttgcaatcc
    12181 atgcacatcg aggatggcat tcccgttgtc tgttacaaat ccctcccgta acacgggttg
    12241 gccaccaagc cgcgtgatct cacgcgctac atagcctcgc gccataggaa tgatctccac
    12301 ggggagcggg aatttgccga gaatgctgac cagcttgcct tcgtccgcga tgcaaatgaa
    12361 tttttttgcc accgccgcaa cgattttctc gcgtgtcaat gcaccccccc ctcccttgat
    12421 catatgcaaa tgctgggtaa tttcatcggc cccatcaacg taaacgggga ttgcatcaac
    12481 actgttcaaa tccatgacct cgatgccata cgcagcgaga cgctgagccg ttgcttgcga
    12541 acttgccaca gctccattaa ttctattctt gatactggat aactcgtcta tgaaatagtt
    12601 tgcggtagag ccggtgccga cgcccaccac gcaagcggct ggaatatgct gaatggcagc
    12661 gtgggcggcg gcacgtttgt ttctatcctg cgacatttat ttattttgaa ggagacagaa
    12721 aaataactta caggataatc ctatccagaa attttaacaa tcaagctcga cgtttacatg
    12781 cgattacgca tgttaaaatc ctgctttcgc gccttcttaa gatgccagtt caatgaaaaa
    12841 cggttatctg gaaagaatcc ttaccgccca ggtctatgac gttgcaatcg aaagtccact
    12901 ggagctcgcg cctaacctgt catccaggat gggcaacagc ttgtggatca aacgcgagga
    12961 tgttcaggaa gtgttctcgt tcaaggtacg cggcgcatat aacaagatgg tcaagctccc
    13021 gcccgaggcg ctcaagcgcg gcgtgatcac cgcgtctgcc ggcaatcatg cgcagggggt
    13081 ggccctcgcg gcccaacgtc tgaattgccg ggcaacgatt gtgatgcctg tgacgacacc
    13141 gcaaatcaaa atgcaggcgg ttcaggctcg aggcgcgacg gtcattaccc atggggactc
    13201 ttatgacgag gcctacgctc aggcgcggaa actcgccgac gagcagaata tcactttcgt
    13261 ccacccctat gacgacccgg acgtcattgc agggcagggc accatcggaa tggagatttt
    13321 gcgccagcac cggggacctg ttcacgcaat atttgccccc gtcgggggag ggggtctgat
    13381 ctccggaatt gctgcctacg tcaaaaggct ttaccccgag atcaagatca tcggtgtcga
    13441 acctgtcgat gcggattcga tgtatcaatc ccttcagaaa aatcgcctgg tcacgcttcc
    13501 tcaagtgggg ctgtttgccg atggtgtagc cgtcaagcag gtcggagtgg agacccttcg
    13561 tttgtgccgc gagctcgtgg atgaaattat tctggttgat actgatgcca tctgcgccgc
    13621 gataaaagac attttcgagg actgccgtgc aattctggag ccctccgggg cattgtcgat
    13681 cgcaggagcg aaagcgtatg ccaagcgtga gcatatcgag gggaaaaacc tcgtggcgat
    13741 cgcgtccggg gcgaacatga attttgaccg tttgcgccat atatccgagc gtgccgaact
    13801 gggggagcat cgcgaagccg taatggcggt aacgattccc gaggagccgg gcagcttcaa
    13861 gaagttctgc gccatgctcg gggcaaaaag catcaccgag ttcaactatc gttacgccga
    13921 tccaaaggaa gcgcatgttt ttgttgcggt ctcggtgcgc gaccgggagg aggcagcaag
    13981 cctgataaaa agcctggttg aaagcggact tgatacggtg gaccttagcg ataatgagat
    14041 ggctaaattg catatacgtc atcttgtggg gggacgagcc cgagaggtaa agaatgaaat
    14101 gttgtatcgg ttcgaattcc ccgatcggcc cggggcgctc atgaaattcc tgaacagtat
    14161 gagtcatcac tggaatatca gcctgttcca ttaccggaat catggcgctg actacggccg
    14221 ggttctggtc ggaatccaag tgccgccgga agagaaggct gaattccgag cgttccttag
    14281 ccagctcgac aaccattact gggatgaaag caataatccg gcctacaagt tatttctggg
    14341 ataaggacgg tcagctggag cgaggggcaa aagcggtgtt ttacgaagga aaaaaggacg
    14401 agcaaaccga atacaggaag gagaatgccg
//
LOCUS       DASSHJ010000090         7151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_140201, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000090 DASSHJ010000000
VERSION     DASSHJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140201"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..507)
                     /locus_tag="VFI43_03660"
     CDS             complement(124..507)
                     /locus_tag="VFI43_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061257.1"
                     /translation="MAIGWMTALKIIPWSNVLESAPHIVKAAKHLFAATKADRDNFTT
                     GYNPPPGDDITPLNRRIQVMEAKIAELREEQKSSAELIKSLAEQNAFVVEAFEVLRLR
                     LKMLLVVCVLLCGALAGLIVWLATR"
     gene            complement(561..1448)
                     /gene="sucD"
                     /locus_tag="VFI43_03665"
     CDS             complement(561..1448)
                     /gene="sucD"
                     /locus_tag="VFI43_03665"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET7061258.1"
                     /translation="MAILINESTRIVIQGFTGKIGTFHANDMISYGSNIVGGVTPGKG
                     GQEHLGLPVFNTVKEAVQETGADATIVFVPPAFAADSIMEAADAGIKYCVAITDGIPT
                     QDMMTVKSFLRRYPTENRMMLTGPNCAGTISPGRALLGIMPARIYTRGNVGIVARSGT
                     LGYEAADQMKMRGIGISTSVGIGGDPIIGSSHKDILEKFEEDEETKAVVMIGEIGGPQ
                     EVEAGVFAKENMSKPVVAYIAGLTAPEGRRMGHAGAIISSAGESAAEKVAMLRDLGVT
                     ICPTPSAIGGTVAKILSGM"
     gene            complement(1449..2639)
                     /locus_tag="VFI43_03670"
     CDS             complement(1449..2639)
                     /locus_tag="VFI43_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HET7061259.1"
                     /translation="MDIHEYQAKDILAKYGVRIAEGGLAYSPEESVQRAREIDGSVWV
                     VKAQIHSGARGKAGGIKVCRSHDEVRDAAEELLGKNLVTRQTGPAGKSCTRVYVEAGV
                     QIVKEMYLCFLIDRSFERVVMVGSAAGGMEIEELTRTNPSAVKKIYIEPAVGLQDFQA
                     REMAFALGVAATQLSHAIKIIRGCYRAMRDLDATMVEINPLIVTATGELIALDAKMSF
                     DDNALFRRHEIAELRDKTQGDPREVAAGDHGLSYIGLKGDIGCMINGAGLAMATMDMI
                     KLAGGEPANFLDVGGGASAERTEKAFRLVLADKGVKAILVNIFAGINRCDWIAEGVVH
                     AVRNIDLKVPLVVRLSGTNVEEGRRIISESGLPIITAGTLAEAANKVVQARNQVAIEE
                     VEGV"
     gene            complement(2767..3717)
                     /locus_tag="VFI43_03675"
     CDS             complement(2767..3717)
                     /locus_tag="VFI43_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7061260.1"
                     /translation="MSHTLYEASVLRVQRCELAVPGSNPGMFEKAMKSGADFVFLDLE
                     DAVAPDDKLSARANVIEALNDLDWEGHGVTISVRINGLDTQYMVRDVVDLVEKAGHKI
                     DTLLIPKVGVYADVYMVEAMLTQLEMQQGLKKRIGIEALIETALGMANVEDIARKGAS
                     GRLESLHFGVADYAASNRARTTNIGGLNPDYPGDQWHAALSRMTVACRAYGLRPIDGP
                     FGDIKDPAGYKQAAKRAAALGCEGKWAIHPSQIELAKEVFTPPSTEIEKAQRILDALK
                     EAAAQGRGAAALDGRLIDAASARMANNVVRMAEAIGVKAV"
     gene            complement(3977..4975)
                     /gene="mltG"
                     /locus_tag="VFI43_03680"
     CDS             complement(3977..4975)
                     /gene="mltG"
                     /locus_tag="VFI43_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7061261.1"
                     /translation="MRTLRRLLYLGAAGILLFVGWVAYQVNDPVQPPALPYEFSIEAG
                     TSLKTVASQLARDGLLPNAWSFVLVSRLMGLASSLKAGDYEITGDISPLELLKRITRG
                     DIAQSEIRFVEGWTFSQLRQALDQHSSIRHDTAGLTDRQVMKLIGATETAAEGLFFPD
                     TYFFARGSSDVEILKRAYHAMQKHLVTAWAGRSGGLPLQDPYQALILASIVEKETGKG
                     SDRGMVAGVFLNRLRASMLLQTDPTVIYGLGDKFDGNLRKKDLLTDHEYNTYTRRGLP
                     PTPIALPGFASIQAVMNPAKTDALYFVAKGNGESHFSRNLEEHNRAVWKYQKNQKR"
     gene            complement(4998..6242)
                     /gene="fabF"
                     /locus_tag="VFI43_03685"
     CDS             complement(4998..6242)
                     /gene="fabF"
                     /locus_tag="VFI43_03685"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7061262.1"
                     /translation="MSKRRVVVTGLGIISPVGNTVSDAWGNVLAGRSGITRITRFDAS
                     AFSSRIAGEVKDFDITHYLSVKDARRMDIFIHYGMAAAIQAVKDAGIGEMENNLDPER
                     IGVNIGSGIGGLPMIEGTHDAYHASGPRKISPFFIPSTIINMIAGNLSIMFGFKGPNL
                     AIVTACSTATHCIGDSARLIEYGDVDVMVAGGAESCVTPLAMGGFASARALSTRNDDP
                     VTASRPWDKGRDGFVLGEGAGVLVLEEMEHAKRRGARIYAELAGFGMSADAYHMTAPC
                     EDGEGAARCMANALRNAEMSPDEVDYINAHGTSTPLGDIAETIAVKRCFGEHAKKIAV
                     NSTKSMTGHLLGAAGGVEAIFSALAIHHQIAPPTINIFDQDEQCDLDYVPNTARSMKI
                     NVGMSNSFGFGGTNGTLIFRKV"
     gene            complement(6293..6529)
                     /gene="acpP"
                     /locus_tag="VFI43_03690"
     CDS             complement(6293..6529)
                     /gene="acpP"
                     /locus_tag="VFI43_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7061263.1"
                     /translation="MENVEQRIKKIVAEQLGVNESEVKNESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDEQAEKINTVQQAIDYINSHTN"
     gene            complement(6756..>7151)
                     /locus_tag="VFI43_03695"
     CDS             complement(6756..>7151)
                     /locus_tag="VFI43_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7061264.1"
                     /translation="IFRLSRGVLRAMMKARYGRIINISSVVGSTGNLGQANYAAAKAG
                     MVGFSKSLAREVGNRNITVNCVAPGFIETDMTRLLTLQQQQNLIQHVPLGRLGRPEEV
                     ASVVGFIASPGASYITGATIHVNGGMHMD"
BASE COUNT         1651 a         2102 c         1693 g         1705 t
ORIGIN      
        1 ctccctcata cccagtaccc gtccaggtat ccccataggg ccaacccgaa ccccgcctag
       61 cctaacccgc tacatacggc tgtgtcaagg agatcgacgg gcaatcgatt gtcaataccc
      121 tgcttacctc gtcgccagcc aaactatcag tccagccagc gccccgcaca gcaaaacgca
      181 aaccacgagt aacattttaa gccgaagccg gagcacctcg aaggcttcaa cgacaaaggc
      241 attttgttca gccagtgatt tgatcagttc ggcagaggat ttctgctcct cccttaactc
      301 ggcgattttc gcttccatga cctgaatgcg cctgttcagc ggggtaatat catcccctgg
      361 aggcggattg tagcccgttg tgaagttatc ccggtcagcc tttgttgccg caaagagatg
      421 cttcgcggcc ttgacaatgt gcggcgcgga ttccaggaca tttgaccagg gaataatctt
      481 taatgccgtc atccagccga tcgccatctc tcaaccctcc agcgtcaatc gtcgcgttga
      541 acagttagcc gaagtccaat ctacattccc gataatattt tcgcaacagt tcctccaatg
      601 gcggagggag tgggacagat ggtaacgccc agatctctca gcatcgccac cttttctgcg
      661 gcgctttccc cggcggaaga aataattgca cccgcatgtc ccatacgccg cccctctgga
      721 gcagtcaacc ccgcaatata agccacgaca ggtttgctca tgttttcttt agcaaatacc
      781 cccgcctcga cctcctgagg ccctccgatc tcgccaatca tgacaacggc ttttgtctct
      841 tcgtcctctt cgaatttttc caggatatcc ttatgcgaac tccctatgat cggatctcca
      901 ccgatcccca ccgatgtcga aatgccgata ccccgcattt tcatctggtc agcagcttcg
      961 tagcccaagg ttccagaacg ggcgacaatc ccgacgttac ccctcgtata gattcgggcc
     1021 ggcatgattc caagcaaagc cctcccaggg ctgatggtac ctgcgcaatt gggtcccgtg
     1081 agcatcatcc ggttttccgt cggataacga cgcaggaagc tcttgacggt catcatgtcc
     1141 tgagtcggaa tgccatccgt gatcgcaacg caatatttaa ttcccgcatc ggcggcttcc
     1201 atgatggagt cggcagcaaa tgccggcggg acaaaaacaa tggtagcgtc cgctccggtt
     1261 tcttgcacag cttcttttac cgtattaaaa accggaagtc cgagatgttc ctggccgcct
     1321 ttcccgggcg ttacgccgcc cacgatgttg gacccatagg aaatcatgtc gttggcatga
     1381 aacgtgccaa tttttcccgt gaagccctgg ataacgatac gggtgctttc gttgatcaaa
     1441 atggccattt aaactccctc gacttcttct atggcgacct gattgcgcgc ctgcaccacc
     1501 ttattcgcgg cttccgccag cgttcctgcc gtaatgatgg gtaatccgct ctcggagatg
     1561 atccggcggc cttcttcgac atttgttcct gataaacgca cgaccagcgg gactttcaga
     1621 tcgatgtttc ttacggcatg aacgaccccc tccgcaatcc agtcgcaccg gttgattccg
     1681 gcaaatatat tgaccagtat ggccttgacg cctttgtcgg ccaacaccag ccggaacgct
     1741 ttttcggttc gctcggctga tgccccccca cccacgtcaa gaaaattcgc gggttctccc
     1801 cccgccagct tgatcatgtc catagttgcc atggcaagcc cggctccatt tatcatgcat
     1861 ccaatgtccc ctttcagacc gatatagctc aacccgtgat cgccggctgc cacctcacgg
     1921 ggatcaccct gtgttttatc gcgcaactcg gcgatttcgt ggcgacgaaa cagagcattg
     1981 tcatcgaaac tcatcttcgc atccagcgcg ataagctctc cggtggccgt gacaatgagc
     2041 ggattgattt ccaccatggt cgcgtccaga tcacgcatgg cgcgatagca gccccgtatg
     2101 atcttgatcg cgtggctcaa ctgggtagcc gcgaccccga gagcaaaagc catctcccgc
     2161 gcctgaaaat cctgcaatcc gacggcaggt tcgatataga tctttttgac ggcagacgga
     2221 ttcgtacggg tcaattcctc aatttccatc cccccggcgg ctgaaccgac catcacgacc
     2281 cgctcgaaac tacggtcgat cagaaagcaa agatacatct ccttgacaat ttgcactccg
     2341 gcctcaacat aaaccctcgt gcacgatttg cctgccggcc cggtttgacg agtgacaagg
     2401 ttcttgccca gcagctcttc cgctgcatct cgaacttcgt catggctcct gcatactttt
     2461 atgcctccgg cttttcctct tgctccagaa tgaatctgag ccttgaccac ccatacgctc
     2521 ccgtctattt ccctggctcg ctgtacgctt tcctccgggc tgtaggccag accgccttcc
     2581 gcgattctga caccgtattt tgccaatata tccttggcct ggtactcatg aatgtccaat
     2641 gcgtcaccca gtttaaaaat tttttatttg gtagagaacg gtaggctgca cgagcatccg
     2701 gttagcggtt gacaactgcg gcccatccgg cggataaaag aaaggccgct aagcggccca
     2761 tagcaattat acagctttca caccgattgc ctctgccatt cttaccacat tgttagccat
     2821 tcttgcagag gcagcatcaa tcaacctgcc atccagcgcc gcagcccctc tgccttgggc
     2881 cgccgcctct ttcaatgcat cgagaatacg ttgtgccttc tcgatttcag tggagggcgg
     2941 tgtgaatacc tcttttgcaa gctcaatctg tgagggatga atcgcccatt tgccttcaca
     3001 gccaagtgcg gcagcccgtt tggctgcctg tttatagccg gcagggtcct tgatatcgcc
     3061 aaacgggcca tcgatcggac gaagaccata ggcacggcag gcgacagtca tgcgactgag
     3121 cgccgcgtgc cactgatcgc ctggatagtc cggattcaac cctccaatgt tcgttgttct
     3181 cgcgcggttg ctggcggcat aatcggcaac accgaaatgc agtgattcaa gcctgcccga
     3241 cgcccctttc cgggcaatat cctccacatt ggccatgcca agcgctgtct cgatcaatgc
     3301 ctctattcca attcttttct tcaatccctg ctgcatttcc aattgggtca gcattgcttc
     3361 aaccatataa acgtcagcat acacaccaac cttgggtatc aatagagtat caatcttgtg
     3421 gcccgccttt tcgaccagat cgaccacgtc gcgaaccatg tactgggtat ccaatccgtt
     3481 tatgcgtacc gatatcgtga caccatggcc ttcccagtcc agatcgttaa gggcctctat
     3541 tacatttgca cgggctgaaa gtttatcgtc gggagcgact gcgtcctcaa gatcaagaaa
     3601 gacaaagtcg gcaccgcttt tcatcgcttt ctcgaacata cccgggtttg aacccggcac
     3661 cgccaattca cagcgttgaa cgcggagaac cgaagcctcg tacaatgtat gactcatttg
     3721 cttcttcctt tgctattgat tgaacagaag ggggaacgta aatttttgaa cttcctggcc
     3781 gttcgacagg gagatgacca ccactttcaa aaaagcgcaa ttctactttt gaaggtggta
     3841 atgtcaattt ataacctgtg agcggatgac aggagcgaag gatcgagggc ccagtgacgc
     3901 actggcagga agcctgattc agctcttata gacgttggaa taatcccccg tttcgtgaaa
     3961 ccttcagaca atccgcttac cttttctgat ttttctgata tttccatact gcccggttgt
     4021 gttcttcgag gttcctcgag aaatgggatt ctccattccc tttggcgaca aaatatagcg
     4081 catcggtctt cgccggattc atgactgcct gaatggatgc gaagccggga agcgcgatcg
     4141 gggtaggcgg caggccccgg cgcgtatagg tgttgtactc atgatcggtc aacagatctt
     4201 tcttgcgcag gttgccgtcg aacttgtcgc ccaacccgta gattaccgtc ggatcggtct
     4261 gaagcagcat gcttgcgcgc aacctgttca gaaatacacc cgcgaccatt ccacggtcgc
     4321 tccccttgcc agtctccttt tcgacaatcg acgccagaat cagcgcttga taagggtctt
     4381 gcagcggcaa gcctcccgag cgcccggccc aggcagtaac aagatgcttt tgcattgcgt
     4441 gataggcgcg cttcagtatt tccacgtcgc tgctgccgcg ggcaaaaaaa taagtatccg
     4501 gaaaaaagag tccttctgct gccgtttcgg tcgcaccaat caacttcata acctgacgat
     4561 cagtaagacc tgcagtatcg tggcggatgg aggagtgctg atcgagcgcc tgtctaagct
     4621 gagaaaaagt ccagccttcc acaaacctga tttcgctctg cgcgatatcg cccctggtga
     4681 tgcgctttaa cagttccagc ggagaaatat cgccggtaat ttcatagtcg ccggccttga
     4741 gcgaggatgc gagtcccatc aaccgcgata ccagaacgaa tgaccatgcg ttgggcagga
     4801 ggccgtcgcg cgcaagctga cttgcaactg tctttaaact ggtacccgcc tcaattgaaa
     4861 attcataagg gagcgccggt ggctgcacag gatcgttaac ctggtatgca acccatccga
     4921 caaacagaag gataccggcg gcccccaggt aaagcaggcg tcttagcgtg cgcatgcatt
     4981 atggataaaa caaaaaatca gaccttgcgg aagatcaacg ttccatttgt accaccaaaa
     5041 ccaaatgagt tcgacatgcc cacgttaatt ttcattgatc gcgccgtgtt gggcacataa
     5101 tcgagatcgc actgctcatc ctgatcaaaa atgttaatgg tgggaggggc gatctggtgg
     5161 tggatggcca aggcagaaaa tattgcctcg accccgcccg ccgcaccgag caggtggcct
     5221 gtcatggact tggtcgagtt cacggcaatt ttttttgcgt gttcaccaaa acagcgcttc
     5281 accgctattg tctcagcgat atccccgagg ggagtggacg tcccgtgcgc attgatgtaa
     5341 tcaacctcgt cagggctcat ttccgcattt ctcaaggcgt ttgccatgca gcgcgctgcg
     5401 ccctcgccgt cctcgcaggg tgctgtcatg tgatatgcgt cagcactcat gccaaaaccc
     5461 gccagttcgg cataaattcg cgcaccacgg cgtttggcat gctccatttc ctccagcaca
     5521 agcacccctg cgccttcgcc gagcacaaat ccgtctcgcc ctttatccca tggccggctc
     5581 gcagtgacag gatcatcatt acgagtcgaa agcgcccgcg ccgaagcaaa accacccatt
     5641 gcaagcgggg ttacgcatga ctccgccccg cccgcaacca tgacatcaac gtcaccgtac
     5701 tcaatcaagc gagcggaatc gccaatgcag tgggtggcag tggagcatgc tgtgacaatg
     5761 gccagattgg ggcctttaaa accgaacata atcgaaaggt tgccagcgat catgttgatg
     5821 atggtgctgg ggatgaaaaa gggggaaatc ttgcgtggac cgctggcgtg gtacgcatca
     5881 tgcgtaccct cgatcattgg caacccgcca atgcccgaac ctatattgac cccaatgcgt
     5941 tcgggatcaa gattgttttc catctctccg atgccagcgt ccttcaccgc ctgaattgct
     6001 gcagccatgc cgtaatggat gaaaatatcc atgcggcggg catccttcac ggacaggtaa
     6061 tgagtgatgt cgaaatcctt tacctctccg gctattctcg atgaaaatgc ggaagcatca
     6121 aagcgagtga tccgggtaat acccgatctt ccggccagta cattacccca cgcgtcggaa
     6181 acggtgttac cgacaggcga aataatgccg agcccggtaa caactaccct gcgcttcgac
     6241 aaaatgactc caaatacatg cgatattcgc gatagttgct tgagggaaca gattaattgg
     6301 tgtgggaatt tatgtagtcg atggcctgtt ggacggtatt tattttttca gcctgctcgt
     6361 cagggatttc acattcaaat tcttcttcca gcgccatgac cagctctacc gtatcaagtg
     6421 aatcggcgcc caagtcatca acaaacgaag actcgttttt gacttcgctc tcattcactc
     6481 ccagttgctc ggccacgatt ttttttatac gctgttctac attttccatc taactgacct
     6541 cttttccggt aagaaaaacg ccgccattct aacaagtttt gtgtattagt aaaatcgaaa
     6601 ccctgacgag attgaaatgc aacgagaaac tctatccggt tctccagact cagcgaaaga
     6661 tgaaaaaatc tcgcagcagc caatgtgata aacacgaagg accaaccgat cgtgccttga
     6721 acaacccgga atccgggcag gctgaaggaa gcgcattaat ccatgtgcat gccaccattc
     6781 acatgaatgg tcgcgccagt aatataactc gcccccggcg atgcaatgaa tccgacaaca
     6841 gaagcaactt cttccggccg tcccaacctc cccagcggaa cgtgctggat caggttttgc
     6901 tgctgctgga gtgtaagcaa acgcgtcatg tccgtttcta tgaatcccgg agcgacgcaa
     6961 tttacggtga tgttgcggtt acccacctcc cgcgcaagtg acttgctgaa tcccaccatt
     7021 cctgctttgg cggccgcata attggcttgt cccagatttc cggtagaccc cactaccgag
     7081 gagatattaa taatgcgtcc ataacgcgct ttcatcattg cccgcaaaac cccccggctc
     7141 aaacgaaaga t
//
LOCUS       DASSHJ010000091         5251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_261824, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000091 DASSHJ010000000
VERSION     DASSHJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261824"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2815)
                     /locus_tag="VFI43_03700"
     CDS             complement(<1..2815)
                     /locus_tag="VFI43_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7061265.1"
                     /translation="MLRRALKLFALSVLFLLLVLSCVAGFLAYREIETSAYQASRLSK
                     LADELRWEIKEGANTEPLLSQAGPYDVRLGYSRLPELLQHLTNYGFQVHAQAHVSPRM
                     RHLVRQGLFVPYREKSQAGLRIEAHDGLPLYRATFPARVYENFETVPSIVRDSLLFIE
                     NRELLDLANPTKNPAVDWPRLGKAVLDKMIQLYRPEHDVSGGSTLATQIEKYRHSPNG
                     MTLTATDKLQQVASASVRAYLDGENTLSARKRILVDYVNTVPLAAAPGFGEANGLGDG
                     LWAWYGLDFEETNHLLNSRFVEGDALVEAASRYKHVLSLMIAQRRPSYYLLGGRKELA
                     ELTDSHLRVLAQAGVITPGLRDAALNLPLQFREAAAPEEIRNFAAQKAVNAVRVRLAS
                     QLGLQRMYDLDRLDLAVQSTLDGELQKKITDMLRQLRDKEYAQTVGLYGHRLLNEDDD
                     PAKIIYSFTLSELTPEGAKFRIQADSFDQPLDINKGTKLDLGSTAKLRTLVTYLEIIE
                     ALHDKYSMLEPRSLRKQDVDPSDVLSRWAIDYLLQTADKSLGAMLNAALERKYSGKPD
                     ERFFTGGGLHVFHNFKREDDRRVWTVREATLASVNLVYIRLMRDIVRHYMFHIVGSSA
                     RILKDVSDTHRTQYLRRFADKEGKEFINRFYAKYRAKAAKANDTAEIFLSSFRHTPRR
                     LAAAYRYIYPEATPAQFQKFMAPHMAGIKGITPTVLGELYENYAPGKYSLADQGYIVT
                     VHPLELWLVRYLIDNPHAQYKDVIEASGEERVAVYQWLFKTIHKNAQDARIRGLLELE
                     AFLEVHRNWKRLGYPFNSLVPSLATAIGSSADHPAALAELMGIILNNGVRVPPVLVER
                     MRFAAGTPFETMVERTPVKGERMFSPELATAVRGVLSGVVESGTASRLARAIVEEDGA
                     ETLIGGKTGTGDHRYVTFSSPGVI"
     gene            3500..3805
                     /locus_tag="VFI43_03705"
     CDS             3500..3805
                     /locus_tag="VFI43_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7061266.1"
                     /translation="MTKINVLLTAAALAALAGCSKTDTYTPAENASGEDIFYTNCTKC
                     HKPEAGSVMLLSSSMSNKDAIIQKVQKGSMGMPAFPNIKGEPAQRLAEFVLANSKPK"
     gene            4174..>5251
                     /locus_tag="VFI43_03710"
     CDS             4174..>5251
                     /locus_tag="VFI43_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET7061267.1"
                     /translation="MTRPLLTRRLLIALPVVIPMLVMLAAGTIWIALRGSLPQYEGAL
                     DIPGLAAPVSVERDKLGSVTIRAQERHDLAWALGYVHAQERFFEMDLMRRRAAGELAE
                     LFGAGALPADRKSRVHRMRARAVAALAALPVSQRQLIDAYRDGVNQGLDALAVRPFPY
                     LLTQTQPVPWRSEDSILVVKAMYFTLNDASNERELALSTMRGALPDVAYRFLTASGGE
                     WDAPLIGPAFRWPDPPSAVELDLRNLDPSLLRQDSGEYADTIPGSNGFAVSGQLTNGS
                     ALVANDTHLELRVPNIWFRTRLIYPSSQRPETVNDHIGVGLPGTPAIAIGSNRQIAWG
                     FTNSYGDFTDWVRVRLDPSDPNRYR"
BASE COUNT         1168 a         1466 c         1464 g         1153 t
ORIGIN      
        1 tgataacgcc gggcgaggag aatgtgacat aacggtggtc gccagtgccg gtctttccgc
       61 cgatcaatgt ttcggctcca tcctcttcca cgatcgcgcg cgcaagccgc gacgctgttc
      121 ccgattcgac aacgccgctc agtacgccac gcaccgcggt cgcaagttcg ggagaaaaca
      181 tcctctcgcc cttgacaggc gttcgctcga ccatcgtttc aaatggcgtg cctgctgcaa
      241 aacgcatcct ttcgacaagc acgggtggaa cccgcacacc gttgttaagg atgatcccca
      301 tcagttccgc aagcgctgcg gggtgatcgg ccgaactgcc aattgcagtt gcgagcgacg
      361 gcaccaggga gttaaacgga taacccaggc gtttccagtt gcgatggacc tccaggaacg
      421 cctcaagctc gagcaagcca cgaatccttg cgtcctgggc attcttgtgg atggtcttga
      481 atagccactg gtagacggcc actcgctctt cgccgcttgc ctcgatcacg tccttgtact
      541 gggcatgggg attatcgatg agatagcgaa ccagccagag ctcaagcggg tgaactgtta
      601 cgatatagcc ctggtcagcc agggaatatt ttcccggcgc gtagttttcg taaagctcgc
      661 cgagtacagt cggcgtaatc cccttgatac cggccatgtg gggagccatg aatttctgga
      721 actgggcagg cgtcgcctcc ggatagatat aacggtaagc ggcggcgagg cgccgaggcg
      781 tatgacggaa gctggaaagg aatatttcag ccgtatcatt cgctttggcg gccttggccc
      841 ggtacttggc ataaaaccgg ttgatgaatt ccttgccttc cttgtcggca aagcgcctga
      901 ggtactgggt gcggtgcgta tcgctcacat ccttcagaat cctggcggac gaaccgacga
      961 tatgaaacat atagtgccgc acgatgtccc tcatcaggcg gatatacacg agattgaccg
     1021 aggcgagcgt tgcttcccgg acggtccaca ccctgcgatc gtcttcccgc ttgaagttat
     1081 gaaagacatg caggcctccg ccagtaaaaa agcgctcatc gggtttgccg gagtacttgc
     1141 gctccaaggc cgcattcagc atcgcgccca ggctcttatc cgcggtctgc aggagataat
     1201 cgatggccca gcggctcagg acatcgctgg gatcgacatc ctgcttgcgc aacgacctgg
     1261 gttccagcat cgagtatttg tcatgcagcg cttcgataat ctccagatag gtgaccagtg
     1321 tccgcagctt tgccgtggaa cccagatcca gcttcgtccc tttgttgata tccagcggct
     1381 ggtcgaagct atccgcctgg atgcggaact tggcgccttc gggagtcagc tctgaaaggg
     1441 taaagctata gatgatcttg gcgggatcat catcttcgtt aagtaaccgg tgaccgtaaa
     1501 gcccgactgt ctgtgcgtat tccttgtccc ggagctgacg caacatgtcg gttatttttt
     1561 tctgaagctc gccatccagg gtgctctgta cggcgagatc cagcctgtca aggtcataca
     1621 tccgctgcag gccaagctgt gaagcaaggc gcacgcgaac agcgttaaca gctttttgcg
     1681 cggcgaaatt cctgatttcc tccggcgccg cggcctcccg gaattgcagc ggcagattaa
     1741 gggcggcgtc cctgagtcca ggggtaatga ccccggcctg ggctaatacg cgcaggtggc
     1801 tgtcggtgag ttcagccagt tccttccgcc cgccgagaag ataataagat ggccggcgct
     1861 gcgcaatcat gaggctaagc acgtgtttgt agcgggaggc ggcttcaacc agcgcgtccc
     1921 cctcgacgaa ccgcgaattc aggagatgat tcgtttcctc gaaatcgagg ccgtaccatg
     1981 cccacaatcc atcccctaat ccgtttgctt ccccgaaacc cggtgcggcg gcaaggggaa
     2041 ccgtattgac gtaatccacg aggatgcgct tgcgagcgga taacgtattt tccccgtcga
     2101 gataagcccg cactgaagcg gatgcgacct gctggagctt atccgtggca gtgagcgtca
     2161 tcccattggg agaatgcctg tatttttcga tttgagtcgc aagcgtgctt cctccggata
     2221 cgtcatgctc cgggcggtag agctgaatca tcttgtccag cacggccttc cccagccgcg
     2281 gccaatcgac ggcaggattt ttcgtgggat tcgcgagatc gagcaattcc cggttctcga
     2341 taaaaagcag gctgtccctt actatcgacg gcacggtctc aaaattttcg taaacacgcg
     2401 ccgggaatgt cgctcgatag agcggtaagc cgtcatgggc ttcaatccgc aagccggcct
     2461 gcgatttctc gcgataaggc acgaacaaac cctgccgtac cagatgtctc atgcgtggcg
     2521 agacgtgtgc ctgcgcgtga acctggaatc cataattggt gagatgctgc aacagttcgg
     2581 gcaggcggct gtagcccagc cgcacgtcat acggccccgc ctgtgaaaga agaggctcgg
     2641 tgtttgcccc ctctttaatt tcccaacgca gctcatccgc aagcttggac agccggcttg
     2701 cctggtaggc ggaggtctcg atttcccggt acgccagaaa tcctgcgaca caggataaga
     2761 ctaaaagcag aaacagaacg ctaagagcga aaagcttgag cgcgcgtctc aacatgttat
     2821 gggagtagtg atgggcggaa aataaagggg aggggcgaaa tcgcaaaggc acgccctgtt
     2881 tttcgctgct tgatccagat tataggggga tacgatccgg aaggctcttc tcattgctct
     2941 ctcggttttt atcagcctgg ttcggccttc acgttgtggg cgaggcaaag cagtgccgaa
     3001 tcatagacaa cgagccgagc agatgcaatg aatatagcgg tgagaggcca ttcagaacat
     3061 tgtagggaac gagttacaaa gacaagttac aaagcccggt tacaaagcga agttacaaag
     3121 ccatgagcat gcctgaaact gacaagcgtg ttgcagtaaa tgccggggga ataatgcgtt
     3181 tctcgatgaa gaaatgatag ttcaagaacc cgcaacaatg acgcgtactt agattgtcac
     3241 ctatgcctcg gagttctccg agcttcgaac gtaatgcatc ccgcgcgtct tccacccgct
     3301 cccaagggct ctacagccac ggatttccat gcacgagagc cagatgcatc aatatcaggt
     3361 ccttgctgta gaaccaccat gattgcgcgg actgctgcaa cttccttccg cctctgatta
     3421 tcagagctac ccagcattgg ggagaaggat ataatgcccc ctcccggcaa aataccaact
     3481 ttggaacagg agtataaaaa tgacgaaaat aaacgtattg ttaactgccg ctgcattggc
     3541 ggccctggcg ggttgctcga agactgatac atacacccct gctgagaacg cgtccgggga
     3601 agatatcttt tatacaaact gtaccaaatg ccataaaccg gaagccggca gtgtaatgtt
     3661 gctgagcagc tcgatgtcga acaaggacgc gatcattcaa aaagttcaga agggcagcat
     3721 gggaatgcct gcctttccca acatcaaggg cgagcccgcg caacgccttg ccgagttcgt
     3781 tctggcgaac agcaaaccga aatagaaagc cggatagggt cagcaaaatc gggtcatccc
     3841 ctggctggcc cgacgctagc gatggcttcc gcatctatcg catccaactg agcattgcaa
     3901 ccttgcacgt ctttttaccc aagattatgt aacggcgtgg atggagttcc agcaactcga
     3961 tgctcgcata gctaaaaatc ttgaccatcc tgattgatca cgcatagagc cgcctgaaca
     4021 gctccgatcc cgggacgact gggagcattg tcgaattcat ccggcaaagg caggtttcct
     4081 cctgaatttt tttgcttttg cggtaaaccg gatattgatc aaattgcgga tcggattcga
     4141 ttttcatgcc ccgattttca tggtgcgtgg cacatgacta gaccgctcct aacgcgccgc
     4201 ctgctgattg cattgcctgt tgtaattccc atgcttgtca tgctcgcggc cggcacaatc
     4261 tggattgcgc tgcgcgggag cttgccgcag tatgagggcg ccctggacat cccgggactc
     4321 gctgctccag tatccgtgga gcgcgataag ctgggaagcg tgacgatccg cgctcaggag
     4381 cgccatgacc ttgcgtgggc gcttggctat gtgcacgcgc aggagcgttt cttcgaaatg
     4441 gatttgatgc ggcgacgggc cgcaggcgaa ctggcggaac tttttggagc cggagccctg
     4501 ccagctgacc gaaaaagccg ggtccatcgc atgcgcgctc gggcagtagc agctctcgca
     4561 gcgttgccgg tatcacagcg gcaactgata gatgcttacc gggatggggt taatcaaggt
     4621 ctggatgcgc tcgcggtaag gccatttccg tacctattga cgcaaacgca acccgttccg
     4681 tggcgcagtg aggacagcat cctggtcgtc aaggccatgt acttcacgtt gaatgatgca
     4741 agcaacgagc gcgaactcgc attgtcgaca atgcgcggcg ctttgcccga tgtggcctac
     4801 cgctttctca ccgccagtgg cggcgaatgg gacgcgccac ttattggccc tgcgtttcgt
     4861 tggccggatc ctccttcggc tgtcgaactg gatttgcgca atcttgatcc gtcactattg
     4921 aggcaggaca gcggcgaata cgccgacacg attcccggaa gcaacggttt cgccgttagc
     4981 ggtcaactga ctaatggctc cgcgctcgtt gcaaatgaca cccatctgga attacgcgtg
     5041 ccgaacatct ggtttcgtac ccgcctgatc tatcccagtt cgcaacgccc ggaaaccgta
     5101 aatgatcata tcggcgtcgg ccttcctggc acccctgcaa ttgccattgg ttctaaccgg
     5161 cagattgcct ggggtttcac caacagttat ggggatttca ccgactgggt gcgcgtgaga
     5221 ctcgacccgt cggaccccaa tcgctatcgt g
//
LOCUS       DASSHJ010000092         8687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1521364,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000092 DASSHJ010000000
VERSION     DASSHJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521364"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            864..1160
                     /locus_tag="VFI43_03715"
     CDS             864..1160
                     /locus_tag="VFI43_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061268.1"
                     /translation="MAVDLDHEPAGEVAAIRELARSGWAKTMVFSGLDNQAACKEAIA
                     AGVAGLNGKQREALETIPKAIEKLHKGQFWEDAVGATRQFIWHQALCRMGSRHS"
     gene            complement(1437..3050)
                     /locus_tag="VFI43_03720"
     CDS             complement(1437..3050)
                     /locus_tag="VFI43_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET7061269.1"
                     /translation="MAQVKTVFMFVIAALALSAEAAFAKNDRDLLEGLENWFKKNVRD
                     VEEGHDIWFKSTFGGERFFSLILPNPPFNLPLGFDQMLTSPRDSRFNEYGVINDPDCT
                     AGDATTGYLDKCADPQSAGVIGIRKFPNPSGGAPLIGVACAACHAGFDPVHPPANPNH
                     PHRKNIHPTIGNQYLQIGKIFKANLSPHDPRYQIFSSWPQGTVDTTAIESDHINNPGM
                     ITPIWSVPDRPFFDVTINGEPARVHRNGQGGEDDAGCELAAIRVYFNIGMCAGECMVG
                     HLSNGPGGSQTPIDLAACRKACPELVQAEESVGKLCAFLQIPRPPRLVHAPRGRHHVD
                     WEVVGKGKKVFSEACASCHSNRERLVRHNVLSDDLIHPVSEIGTNSCRARTTNWTAGH
                     IWAPFSSDEYKARPTGGPGFYRDMPLVGIWATAPFFHNNRLGQKFGDPSVAGRIAAYE
                     DAMDLLLNPEKRDEQGSIQRASDSVQLPTASGVVTLPAGTPIAQFANVDPNSGTNLCP
                     DLVENQGHHFGAELSEEDKYALREFLKTR"
     gene            3408..3764
                     /locus_tag="VFI43_03725"
     CDS             3408..3764
                     /locus_tag="VFI43_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061270.1"
                     /translation="MSAESNLDFLEGRALREGLTRTEVIKAHSSSDSFRRSGDYEAPN
                     YVDREINGWLLTSPDIVCVQELKGACGESRLLYNSQKRLVIQYGQGRVIERGSAEPPF
                     MLAMMMRPDKRRIHRN"
     gene            complement(4655..4728)
                     /locus_tag="VFI43_03730"
     tRNA            complement(4655..4728)
                     /locus_tag="VFI43_03730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4694..4696),aa:Cys,seq:gca)
     gene            complement(4866..4940)
                     /locus_tag="VFI43_03735"
     tRNA            complement(4866..4940)
                     /locus_tag="VFI43_03735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4906..4908),aa:Gly,seq:gcc)
     gene            complement(5108..5752)
                     /gene="pgsA"
                     /locus_tag="VFI43_03740"
     CDS             complement(5108..5752)
                     /gene="pgsA"
                     /locus_tag="VFI43_03740"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7061271.1"
                     /translation="MPINLPIVLTWMRIVAIPLFVGIFYCPPTWLSGSDQNLIATIIF
                     TVAALTDWLDGYLARALRQTSAFGAFLDPVADKLMVAAALIVLVYLSRLDALIAFIII
                     GREITISALREWMAQIGRGKNLAVSFLGKLKTTSQMAAIPLLLYHDKIGESFNPQEIG
                     TWLIYLAAILTLWSMGYYLKVAIPQALRHESRRYDRVPRRRRATRPAGSPAEGG"
     gene            complement(6018..7838)
                     /gene="uvrC"
                     /locus_tag="VFI43_03745"
     CDS             complement(6018..7838)
                     /gene="uvrC"
                     /locus_tag="VFI43_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7061272.1"
                     /translation="MLTGPSFDPKEFCSTLPSQPGVYRMLNGAGQVIYVGKAIDLRKR
                     VSSYFQKNGLAPRTRLMVSQVAGIETTVTRSEAEALLLENNLIKSLAPRYNILFRDDK
                     SYPYVILSGHRFPRLGFYRGPLDKKHHYFGPFPNAGMVRESIQLLQKVFRLRTCEDTV
                     FSNRTRPCLLYQIRRCSGPCVDLVSEAVYREDARNAELFLDGRQTEVLQGITTRMKEA
                     ADAQEYEQAALFRDQIQSLRKISEKQFVDSGKALDADVVACVSENNGGGRICVNLAMI
                     RGGRHLGDKSFFPQNAEGYDAPEVVEAFLAQHYLNRSTPGLIIVGEKIRRETLQVLLA
                     EQSGGKVVINTNPIGNKRVWLNMATENARLALEQMLSRQASQEERLQALQQALDIPGL
                     NRIECFDISHTLGEATVASCVVYENFSMRNSEYRRYNIEGIVPGDDYAAMRNVLCRRY
                     QKIAEGEGLLPDLILIDGGRGQVNTAKEVLVELGLNDANVLGVAKGEERKPGLEQLIF
                     PGVEKPLQLGKDHPGLHLIQQIRDEAHRFAIYGHRAKLGKARTRSSLEEIGGIGTKRR
                     QSLLARFGGLKGVQKASIEELQQVGGISRALAEKIYRELH"
     gene            complement(8107..8295)
                     /locus_tag="VFI43_03750"
     CDS             complement(8107..8295)
                     /locus_tag="VFI43_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HET7061273.1"
                     /translation="MPYVNIRIAGSLSSQQKKQIAQEFTDTLERVAHKPKSYTYIAFD
                     ELPHESWAIGGKLLGQED"
     gene            complement(8298..>8687)
                     /locus_tag="VFI43_03755"
     CDS             complement(8298..>8687)
                     /locus_tag="VFI43_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7061274.1"
                     /translation="ITNAVKCLPPENKPAPAEVRQCNHYLSAEIAEFRRREGKGLLAL
                     GAIAHHAVLMALGLKRSIYSFSHGAIHALPGDKASGRSENRNLTLYDSYHCSRYNTQT
                     RRLTAEMFEQVFEKIRADITLHQKTGD"
BASE COUNT         2007 a         2377 c         2207 g         2096 t
ORIGIN      
        1 gtgtcacggc cattaaagcc caggatggta tctgctgccg gtgtgccgtg tagagtatcg
       61 tcgttgttgg tgccgaatat tagtgccatg atatctcctc ctgtttgtga aaaaacttct
      121 ccgtttcccc gtcttgtatt aacttcgggc tatccgcctc ttccttttct ttcgtaatca
      181 ttaccgtcag gatcttgcca agcagaaggg ccattctcgt tatgctccgc tagtcaagcc
      241 agaccccacc attttcgccg cttaccttgc cgtgtttaaa acctttcgag gatgtcatcg
      301 atgagtgtga atggccataa tcttcctcta aggcctctga tgcacataaa gaccgactta
      361 ttcccttcgt tcctcatttc gcacgctttt gcatccacct cgccagttca gcatggtttc
      421 cctgctgtta tccctcttca gagtaagggc ttgtttccat cccggtacgt cattcttttt
      481 cgatcgattc ttctcgataa tcccaataca gttctacaat agctctctcc cgcaaagaaa
      541 cggttatatt tcggttatat ttcggttaaa tttcggttaa atttcggtta aagcggatgg
      601 aatgtgaaag gaggttcgcc gaactcgagc cgcgaagaaa ccgaaatttg tttttttaaa
      661 ttccaaatca atcctcagga gacaatcaat cttctgactt ttctatcgat tccgtttgct
      721 ccgggaaaca tacggctcag gcgtgatgga gcgatgtgat tgggcgcttg gtttggcagt
      781 cagaaccata ggatgaaagt gctcggaaag ttcacccatt gttcgaaagc cgtccctgaa
      841 ctggaaaagt tgcctccgac cggatggcgg tcgatctaga ccatgagccc gcgggtgagg
      901 tagcggcgat ccgcgaactg gccagatcgg gctgggcaaa aacaatggtc ttcagtggat
      961 tggacaacca ggccgcctgt aaggaggcca tagctgcagg ggtggcgggg ctgaacggga
     1021 aacaacgaga agcgttggaa acgattccga aagcaatcga gaagctacat aaaggccaat
     1081 tttgggaaga tgcggtgggc gcgacaaggc agtttatatg gcatcaggcg ctctgccgga
     1141 tgggctcgcg acattcctag gcctgtgaat cgagaacacc gagggtgcaa aatctcgcaa
     1201 gcgcgctggc tttctcatgt gcagcatgtc ccccttgcga ctggcctcgc gccttagaag
     1261 tgaactcgtg cgcggtgccc ttttgacgga tggcgtgacc tcctttgctt gcaacctggc
     1321 gctgcttcta ttcatccatc cgtcaagcaa atctccgcct gctcgtcccc actgccctat
     1381 ccctcaccat gccagcccag ttcctctcct tgctcatgtg agataaccaa agcaaatcaa
     1441 cgcgtcttca agaattccct tagcgcatac ttatcttcct ccgaaagctc tgcgccaaag
     1501 tggtgcccct ggttttcaac aagatcgggg cagagatttg tgccgctgtt aggatcgaca
     1561 ttggcgaact gggcaatggg agtacctgcc ggaagtgtca caacaccgga agcggtaggc
     1621 aactgcaccg agtcgctggc tcgctggatt gatccctgtt cgtcacgctt ttcgggattc
     1681 agcaacagat ccatcgcatc ctcgtaagcc gcaatccgtc cggcaactga tggatcgcca
     1741 aacttttgcc ccagcctgtt gttgtggaag aaaggcgccg ttgcccaaat gccaacaagc
     1801 ggcatgtcgc gatagaaccc tggccccccg gtcggccgcg ccttgtattc atcagaggaa
     1861 aaaggtgccc agatatgccc tgctgtccag ttcgttgtcc gggcgcggca actgttcgta
     1921 ccaatttcag aaaccgggtg aatgagatca tcggagagca cattgtgcct gaccaaacgc
     1981 tccctgttcg agtgacacga cgcgcacgcc tcggaaaaaa ccttcttgcc ctttccaaca
     2041 acctcccaat ccacatggtg gcgacccctt ggagcgtgta ccaatcgcgg aggacgggga
     2101 atctggagaa acgcgcagag cttcccgacg gattcctcgg cctgcaccaa ctctggacaa
     2161 gctttcctgc acgcggcaag atcaattgga gtctggctcc cgccgggccc gttggacagg
     2221 tgtccaacca tgcattcgcc ggcgcacatg ccaatattga aatagacgcg gattgccgcc
     2281 aactcgcacc ccgcatcgtc ctctcctccc tgcccgttgc gatggacacg agcaggctcg
     2341 ccattaatgg tcacatcaaa gaacgggcga tccggaaccg accatatcgg tgtaatcatg
     2401 cccggattgt tgatgtggtc gctttcaatt gcggtcgtgt ctaccgtacc ctgaggccaa
     2461 ctggaaaaaa tctggtaacg gggatcatgg ggtgaaaggt ttgccttgaa aatctttccg
     2521 atctgcaaat actgatttcc gattgtggga tgaatatttt tcctgtgggg atgattcgga
     2581 tttgcagggg gatgaacagg gtcaaacccg gcatgacagg cggcacaggc gacgccgatc
     2641 agtggagccc cacctgaagg gttagggaac ttgcgaattc cgatgacgcc ggccgactgg
     2701 ggatccgcgc atttgtcgag atagcccgtc gtggcatcgc cagctgtgca gtccgggtca
     2761 ttgatgacgc catactcgtt gaaacggctg tcacgcggcg aggttagcat ctgatcaaaa
     2821 ccgagtggaa gattgaaagg agggttgggt aaaataaggc tgaaaaaacg ctccccccca
     2881 aaggtgctct tgaaccagat atcatgcccc tcttccacat ctcggacgtt tttcttgaac
     2941 cagttctcca gtccctctaa taaatcacga tcattttttg caaaagcggc ttccgccgat
     3001 agagccaacg cagcaataac aaacatgaat actgtcttca cctgagccat aacaacctcc
     3061 ttgttatacc ctagtgagcg ttcaaagcaa ttgatacgct cacgttttac aagcatttgt
     3121 atgcggcgat tgaggcacgc ataagaaact tatccttaca gcgcaaggga cgtgaattca
     3181 tccgactagc cgaactccct tccattattc ctttcgttta tcgcttcatt ttgtcccgta
     3241 acaattcttt atagcaaact tctccaatgt ggaaggggtc gcaaaagatg taaaatgttg
     3301 caccaaccgc gccgcatatt gggtaacggc cattaatcag atttaaagca tggattttgc
     3361 cgccgaggtg ctgttcgacg gcaggcgctt gcgtgcatgg ctgtcgtatg agcgcggagt
     3421 cgaatttaga ctttctggaa ggaagagccc tgcgagaggg tctgactaga acggaagtaa
     3481 taaaggccca cagtagttca gatagttttc ggcgaagcgg cgactacgaa gcacccaatt
     3541 atgtcgaccg tgaaatcaat ggctggcttt tgaccagtcc ggatatcgtt tgcgtgcagg
     3601 aactcaaggg ggcttgtgga gaaagtcgat tactctataa ttctcaaaaa cgactggtca
     3661 ttcaatacgg acagggacgc gtcatcgagc gaggatcagc cgaacccccg ttcatgctgg
     3721 cgatgatgat gaggccggac aaacgacgaa ttcacaggaa ttagccacac gctccgctat
     3781 cccttatccg ctctgtcgga cagttcgttt acatcgactg gcgtcggatt acctaccgaa
     3841 ccgggtgtat gccatttata ccttgaaata aaaaacccgc cgaagcgagt taaacctgca
     3901 ataaaaaaaa cgaccggggt cggtgttcgc caaaccttgt tccaccgcta tttgttacgc
     3961 aatgaagaac taccctcgat agaggctgcc gcctcgtttt cccctccgtt ttgccgggaa
     4021 cgggaatgct taatatagtc gcgtccctca acagatctta cgttagcaag ttgcctttcc
     4081 agccaatacg aaaaatcctt ttctaatccg agagccgtat gtagttcccg aatgttcact
     4141 atctgcacag cgcggcttcc tcccttgaca cttcataccg cgaacggagt tcaacggttt
     4201 ctttctgcat gaaagcaatt cccatgccag taagattagt cccacgggct caggggcttg
     4261 gcgctatatg gccgctccac ttcatttcga tggaaataat tccgacacaa tttccttaaa
     4321 ctcgaatacc gcctttattt tcatctccgc gaacgggttg aaacaggcaa taaaaaaccc
     4381 gctgaagcgg gtcagattct tcctttttat tattgtattg atcagtttga gcgtcggtgt
     4441 accctccatg ccaggagacc aagcccagca agcatcatcc ttaggtctca gattcaggca
     4501 aggagctgtt agccgactct gttgaataga tgacagtatg tgagggcgat ggagaaaagg
     4561 tatccccggt tttgatgtct ggttgtgccg aacgagaaaa agcgtaaaaa tggcgaaaaa
     4621 attttccgcc aattaagtcg ccagtcactg gttttggagg cgggggtcgg aatcgaaccg
     4681 gcgtccacgg ctttgcaggc cgctgcataa ccactctgcc accccgccat tactgcgctg
     4741 ccgaatgccc gcgttgcacc gttgcagtgc gagaagacaa aaaactaaaa agggaaaatg
     4801 gacttttttg ctgaggcttt acttctgaac aacagcctag caaacggatc cattttccca
     4861 gtaactggag cgggaaacga ggctcgaact cgcgacccca accttggcaa ggttgtgctc
     4921 taccactgag ctattcccgc aaaaaaacaa tctggcatta taaggatgca gtcacctttg
     4981 tcaagaaagc gtcagcggcg gcggagggca gagcagtagg gtctgaagga tgatgacggc
     5041 tgggaaaagt gtaaggagtg cacgacgctt ccgatgccac tatgcagcct gacactaggc
     5101 ccgggagtca acccccctcc gctggtgatc ccgctgggcg ggttgcccgc cggcgcctgg
     5161 gtaccctgtc atatcgcctt gactcatgtc ttaaggcttg tggaatggcg actttcaggt
     5221 aatagcccat ggaccataga gtgagtatgg cagcgagata gatgagccat gttccgatct
     5281 cctggggatt aaaactttcc ccgatcttat cgtgatacag caatagcgga atcgcggcca
     5341 tctgggaagt ggttttcagc ttgcccagga aagaaaccgc aaggtttttc cccctgccta
     5401 tctgcgccat ccattcgcga agggctgaaa tggtgatttc ccgcccgatt atgataaacg
     5461 caataagggc atcgagcctg ctcaggtaaa cgagcacgat cagggctgca gcgaccatca
     5521 atttgtctgc gactggatcg agaaatgcgc caaaagctga agtttgccga agcgctcgcg
     5581 caagataacc gtccagccaa tcggtgaggg cggcaacggt aaagataatc gtggcgatca
     5641 gattctggtc agacccggac aaccatgtcg gggggcagta aaagatgccc acaaacagcg
     5701 ggatggcgac tatacgcatc catgtcagta cgattggcag gttaatgggc atgacaatcc
     5761 aggaatatgg caaatagtgt ggttggagag cctggacatg attggcttcc ctgtttttga
     5821 aaatcctgcc tgtcccgatt atcctgcgtc attaccacga tttattctcg agcagatatt
     5881 cagtatgttc ctcaccttat gatagttcat gggtacgaac tattattctt cccgcaccgc
     5941 ctccttttag cgccaaccag cgtgctcatt gcactaagat agatgcaatg agccgagacg
     6001 cattccgcac tcgcgattca gtgtaattcc cggtatattt tttccgccag ggcacgactg
     6061 atcccaccca cctgctgaag ctcctcgata cttgccttct gcacgccctt caatccgcca
     6121 aaccttgcca ggagactctg gcgacgcttt gttccaatgc caccgatttc ttccaggctc
     6181 gaacgggtac gcgccttacc aagcttggcg cgatgcccat atattgcaaa tcgatgggcc
     6241 tcgtctcgaa tctgctggat caggtgcaac cctggatgat cttttcccaa ttgtaacggc
     6301 ttttccaccc ctggaaaaat caattgttcc aatccgggtt tccgctcctc gcccttcgca
     6361 accccaagca cattcgcatc attgagtcca agctcgacaa gaacttcttt cgcagtattc
     6421 acctgccccc tgccgccatc gatcagaatc agatcgggaa gcaggccctc gccttccgca
     6481 atcttctgat agcgccggca gagaacatta cgcatggccg catagtcgtc tcccggcaca
     6541 atgccttcga tgttataccg gcggtactcg ctgttgcgca tcgaaaagtt ttcgtacacg
     6601 acgcaggaag caaccgtggc ttcgccgagc gtatggctga tgtcaaagca ttcgatccgg
     6661 ttgagccctg ggatatccag cgcttgctgc aatgcttgca ggcgctcttc ctggcttgcc
     6721 tgccggctca acatctgctc caaggcaagg cgcgcgttct ccgttgccat gttcagccag
     6781 acccgtttgt tgccgatagg attggtgttg atcaccacct tgcccccgga ttgctccgcg
     6841 agcaacacct ggagtgtttc ccggcggatt ttctctccga cgatgataag accgggcgta
     6901 ctgcggttaa ggtaatgctg ggccaggaat gcctcgacaa cctctggagc atcgtacccc
     6961 tccgcattct gcgggaaaaa gcttttatcc cccaagtgac gcccccccct tatcatggcc
     7021 aggtttacgc agattcttcc cccaccgtta ttctcggaga cacacgcgac cacatcggca
     7081 tccagcgcct tgccgctgtc cacaaactgc ttttcactga ttttccgcag ggactgtatc
     7141 tggtcgcgaa agagcgcggc ctgctcatat tcctgggcat ccgccgcctc tttcatcctg
     7201 gttgtgatgc cctgcaatac ttctgtctgc ctaccgtcaa gaaaaagttc agcgttcctc
     7261 gcatcctccc gatagacagc ctcgctgacc aagtctacgc aaggtccact gcagcggcgg
     7321 atctgataga gcaggcaggg ccgcgtacga ttgctaaaaa cggtatcctc gcacgtgcgc
     7381 agcctgaaga ctttttggag aagctggata ctttcccgca ccatgccggc attgggaaag
     7441 gggccaaaat aatgatgctt cttgtccaac gggccccggt agaacccaag ccgcggaaaa
     7501 cggtgcccgc ttaagatcac atacggataa gacttgtcat ccctgaagag aatgttgtac
     7561 cgcggagcca gcgattttat caggttgttt tcaagcagca gcgcctcggc ttcggagcgg
     7621 gttacggtag tttcgattcc cgcaacctgg gacaccatga gcctggttcg gggggccagt
     7681 ccgttcttct gaaaataaga ggaaactctt ttccgaaggt caatggcctt cccgacataa
     7741 atcacctgtc ccgcaccgtt caacatgcgg tataccccag gctgggaagg aagtgtggag
     7801 caaaactctt ttgggtcgaa actagggcct gttaacactt atttcacgcc tgcgacggtg
     7861 acggatttta gtcagcacga ggcgcgagtc gcgccgcaga gcaagcctct gtaagcggtg
     7921 agcaacgacg ggctgggtaa aatccgtccc gtccttcggg ttgcgcccga aaaaacgtgg
     7981 atgctgcgtt gcaaagcttg acctagttca ctatgtcctg cgcttcgcgc cttaccccac
     8041 gttttttcgt gcagcaacgc aggtcgcgaa ataagtgtta acaggccctg ctccgcggca
     8101 atgcggttag tcctcctgcc ccagcaattt ccctccgatg gcccagcttt cgtgcggcaa
     8161 ctcgtcaaaa gcaatatagg tatatgactt cggcttgtgc gcgacgcgtt cgagcgtatc
     8221 ggtaaattcc tgggcaatct gtttcttctg ctggctgctc agcgagccgg caatgcgtat
     8281 attcacataa ggcacgttta atctccggtt ttttggtgaa gagtgatatc agcacggatt
     8341 ttctcgaaca cctgctcgaa catttcggcg gtcaggcgcc tggtctgtgt attgtaacgg
     8401 ctgcaatggt agctgtcata taaggtcaga ttgcggtttt cggagcgccc ggacgcctta
     8461 tcgcctggta atgcatgtat cgccccatgg ctgaacgagt agatcgacct tttcagtcct
     8521 agtgccatga gcacagcgtg atgcgcgatc gcacccagcg cgagcagtcc ttttccttcc
     8581 cgtcgcctga attcagcgat ttccgcggag aggtaatggt tgcattggcg gacctcggca
     8641 ggagccggct tgttttccgg tggcaggcat ttcactgcat tggtaat
//
LOCUS       DASSHJ010000093         5728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1225221,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000093 DASSHJ010000000
VERSION     DASSHJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225221"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFI43_03760"
     CDS             <1..301
                     /locus_tag="VFI43_03760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7061275.1"
                     /translation="LDMDETDAFLGYLYTWPIFRRQGAARWLIAAAVNGLSTHNVRRV
                     FAHVRATNVPSLAAFERAGWKTGGMLVCTLNGDLLLAPGVARVGLNFRPRDERIE"
     gene            complement(393..2498)
                     /locus_tag="VFI43_03765"
     CDS             complement(393..2498)
                     /locus_tag="VFI43_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7061276.1"
                     /translation="MRLLLVIALLIAYGSLYPGNFSASSPGAVGRFLSNWSWFTSLAD
                     VLGNIALFFPLGAAAMLFTSSRPNTLARTAGMLFLAFLFSFVLQLAQVWLPSRSAALA
                     DVVWNMAGMASGMAAAHGVGRALNRIQPLGSASVIPLLILILWVTAELLPLVPSLDLQ
                     KFRDALKPLLLDFHFSFPQAVMHAAGVIAAGAALAALGRRPGWWLGGFLMFVLAGKLA
                     IVNLVLDASLVIGLGAGYAAFLATLRLGGSKIFHTAFWLLLAAWTLVSMTPFAPAPGG
                     TFNAIPFATMLSGSLETATRGLVQSLFIYTALLWLAEATGTGIRKATVGLIIWSCLIE
                     FTQMGLLGRTADVTEPVLVLLLSWIVPARQRHVEHVESEPKARARQAPVTAVAAEITG
                     ERTLGLLATGIVICASLGWFITQSALTPYNVRELVYEGHPFRSLISLAALLYWSIGFP
                     VLIAKWLTRSGLYLFSLPALVLLHGLVAWVLLRSAVPDESIHDIVGSPVLGWPWDWEL
                     LGRFLALFSFWSVAATAGSLIAAGHILPGAKSALPGWAIGASLLIPFSYYVVVTAAST
                     DNLVELMAHNGSVGSFLLIGVAIAEISFGGTKGALALIPGAPQRGRAVVWVVGAGVLA
                     YAALYFGTEQIIIKYERVFSALQFLLSSDRSHLAGPKELMIRYIALYGFFIVIIVIVQ
                     NPLWRWVVSKHPQGSVRLE"
     gene            complement(2533..3930)
                     /locus_tag="VFI43_03770"
     CDS             complement(2533..3930)
                     /locus_tag="VFI43_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET7061277.1"
                     /translation="MFKQHPFLRLVTPNLFQPMLELNPVLIKCVVLAGVLSHIIPVQG
                     AAIYIANPVNYLSLLRTLRPGDSLQLMPGEYRAGLPVHNLEGTSEAPITISGPDDGPR
                     PVFVARPKHNTVSIINSSHVAVRNLDLDGQNRPVDGVKCEGHADWAHHIILERLRITG
                     HGNNQQTVGISTKCPAWKWVIRNNLIASAGTGLYLGNSDGRAPFIAGVIEYNLIVDTR
                     GYNLQIKHQEKRPELPGMPEQPTATIIRHNVFSKANGGSAKELARPNVLVGDSPLTGP
                     GSSDRYLVYGNFFYENPHEALFQGEGNIALYNNLFVNRSGDAIRIQPHNGSPREISVF
                     YNTVLSMGAGITLARREHDPTYPQFVFANIVFAQAPIRGASFAKNLTGGLEDAEKYLS
                     HPFGPLGELNLTATGVQALGPAQGPAGFETYPGAQQDFDGNSYSGARIGAYMTGKSLP
                     RWMPALQIKPPPAAR"
     gene            4319..5470
                     /locus_tag="VFI43_03775"
     CDS             4319..5470
                     /locus_tag="VFI43_03775"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7061278.1"
                     /translation="MTRFEPARFTREAADVLSSILDNSQYQCATRLPQSGGNRWYSEP
                     VRLAKFATGGVNCWKESPWQGLYQNLLKWEKGFSPVVLFDLFFFHKPVSADRITAVVG
                     AGNLEILVDAGILSSSRGEVRSLVRCYPVGNMYFICDPSRDQSDFVYIGWDSDLMVNI
                     AAKYCRGRRFSRLLDLCTGSGVQALSLSPISEEVFCVDINPRALAMAQANARLNKLPN
                     VNAVHSDLFSNVTGRFDCITANTPYVPHPEGAQLPIGGGDIGIEFTIRLLRELPDRLT
                     EKGISVIYTSDPIVRGKRQLIPRVTAELGHLPFRMILIPLFTNNYPMTRPMQEHYDRL
                     NLSGYDDCILLIEHGKKYEVEHHQHDALHYYRTRMDAWLDWRRRAIRHG"
BASE COUNT         1385 a         1512 c         1555 g         1276 t
ORIGIN      
        1 tctggacatg gatgaaacag atgctttttt aggctattta tacacttggc ccatatttcg
       61 tcgacagggc gccgctcgct ggttgattgc tgccgctgtg aacggccttt ccactcacaa
      121 cgtccggaga gtgttcgctc acgtgcgggc gacgaatgtt ccttcattgg cagcttttga
      181 gcgtgctggc tggaaaacgg ggggaatgct ggtatgtacc cttaacggtg atttattgct
      241 ggcgcccggg gttgcaaggg tagggctcaa ttttcgccct cgagatgaac gtattgagtg
      301 agatagtggg aattggcaac cgagcgtttc ggacaactcc gaaaagcttc aagcgttcat
      361 tattaatagc gtgtgatgac cgggatgagg tgctattcaa gtctgactga tccctgggga
      421 tgctttgata cgacccagcg ccaaagcgga ttttggacga tcacgatgat tacgataaag
      481 aagccgtaca acgcaatata gcggatcatg agctctttcg gtccagcgag gtgcgagcgg
      541 tcgctactca acaagaactg cagtgcggaa aatactcgct catatttgat gattatttgt
      601 tccgtgccga aataaagagc tgcataggca agaactcccg ccccaaccac ccataccacg
      661 gccctccccc gctgtggtgc tccaggaatg agtgcgagtg cgcccttcgt tcccccaaat
      721 gatatctccg cgattgcgac gccgatcagt aaaaacgaac caacgctgcc attatgcgcc
      781 atcagttcga ccaggttgtc ggtggaggct gccgttacga ctacataata tgaaaaaggg
      841 ataagcaggg aggcgccgat cgcccaaccc ggcaaggcgg attttgctcc gggaaggata
      901 tgccctgctg cgataagact ccccgctgtc gcggccacgc tccagaaact gaacaacgcc
      961 aggaaacggc ccagcagttc ccaatcccac ggccatccca gcacgggaga tccgacaata
     1021 tcatggatgc tttcgtctgg tacagcagat cgcagcaaca cccatgcaac cagtccatgc
     1081 aggaggacca gggctggcag actgaaaaga tacaggccgc tccgcgtcag ccacttcgcg
     1141 atcaaaacgg ggaatccgat ggaccagtac agcagggctg caagcgatat cagcgagcgg
     1201 aagggatggc cttcatacac cagttcacgt acattgtagg gcgtcaatgc agactgggta
     1261 ataaaccagc ctagcgaagc acatatcact atgccagttg ccagcaaacc aagcgtgcgt
     1321 tcaccagtaa tctctgcggc gactgcagtg acaggtgcct gccgagcacg tgctttgggt
     1381 tcagactcca catgttccac gtgccgctgc ctagctggca ctatccagct cagcagcagc
     1441 accaaaacgg gttccgtcac atctgcggta cggccgagca ggcccatctg ggtgaactcg
     1501 atcaagcacg accatattat caatcccacg gtcgcctttc tgatacctgt ccccgttgct
     1561 tccgcgagcc atagcagggc ggtataaata aataaagact ggaccaatcc tcgcgtggcg
     1621 gtctccaatg atccagagag catggttgcg aagggaatgg cattgaaggt gcccccggga
     1681 gcaggagcaa aaggtgtcat cgacacgagg gtccatgctg ccagaagcaa ccagaatgcc
     1741 gtgtgaaaga ttttcgaacc gcccaagcgc agcgtagcca ggaacgccgc ataacctgcg
     1801 ccaagcccta tcacgaggga cgcatccaat accagattca cgatagccag ctttcctgcc
     1861 aggacgaaca tgaggaatcc tcccagccac caaccgggcc gccggcccag cgctgcgaga
     1921 gcagcgcctg cggcaattac acccgcagcg tgcatgacgg cttgcgggaa tgagaagtga
     1981 aaatcgagga gaagtggttt cagcgcgtcc ctgaatttct gcagatccag agaaggcacc
     2041 agcggcaata gctcggcagt tacccacaat atcaatatca acaagggaat gacggaagcg
     2101 gaaccgagcg gctgaatgcg attgagggcg cgacccaccc catgcgctgc cgccatccct
     2161 gacgccatgc ctgccatgtt ccataccacg tcggccaagg ctgcagagcg ggaaggcagc
     2221 caaacttgag ccaactggag aacgaatgag aagaggaagg ccagaaaaag catcccggca
     2281 gtgcgagcca gcgtattcgg tctgcttgaa gtaaagagca tggccgccgc tccaagcggg
     2341 aaaaacagcg caatgttgcc gagcacgtcg gctaacgaag taaaccagct ccaattgcta
     2401 aggaatctgc ctacagcacc ggggctagag gcggaaaaat tgccgggata caacgatcca
     2461 taagcgatca gtaatgcgat gactaatagg aggcgcatgg gatacgggta gcatttggat
     2521 cgggacagga cattatcgcg ccgcgggtgg tggcttgatt tgtagtgcag gcatccagcg
     2581 ggggagggat ttgccggtca tataagcgcc tattcgggcc ccgctgtacg aattgccgtc
     2641 gaaatcctgc tgagccccgg gataggtttc aaagcctgcg gggccttgag caggccccaa
     2701 tgcctgaacg cccgttgctg tcaggtttag ttctcccaag ggcccgaatg ggtgagaaag
     2761 gtacttctct gcatcttcga gcccgccggt cagattcttc gcaaaggaag cacctcgtat
     2821 aggcgcttgg gcaaatacga tattcgcaaa gacgaattgc gggtaggttg ggtcgtgctc
     2881 acggcgggca agcgttatcc cggcgcccat ggaaagcacg gtattgtaga acacgctgat
     2941 ttcacgggga ctgccattat ggggctggat gcggatcgcg tcgcccgacc ggtttacaaa
     3001 cagattattg tacagggcga tattcccttc cccttgaaac agcgcttcgt gaggattttc
     3061 atagaagaaa ttaccgtaaa cgagatagcg gtctgacgat cccgggcccg tcaacggcga
     3121 gtctccaacc agtacgttag ggcgagccag ttctttcgcg gaacccccgt tcgccttgct
     3181 gaagacatta tggcggatga tggtcgcagt tggttgctcc ggcatgcccg gcaactcggg
     3241 tcgtttctct tgatgcttga tctggaggtt atagccgcgc gtgtcaacga tcaggttgta
     3301 ttcgatcacg ccagctataa atggcgcgcg tccgtccgaa tttccaagat aaaggcctgt
     3361 gccggcactt gcaatgagat tgtttcgaat aacccacttc caggcaggac acttggtgga
     3421 aattccgacc gtttgctgat tattgccgtg gccggttatg cgtaatcgct cgagaatgat
     3481 gtgatgcgcc caatccgcgt gtccttcgca tttcactcca tccaccgggc ggttttgacc
     3541 gtccagatcg aggttacgta ccgcgacatg gctggagttg atgatgctca cagtattgtg
     3601 ctttggccgc gcgacgaaaa ccggacgagg accatcgtcc ggaccggaga ttgtgatcgg
     3661 tgcttctgaa gttccctcga gattgtggac gggcagaccc gctctatact cccccggcat
     3721 gagttggaga gaatcacccg ggcgcagggt tcttaacagt gaaagataat tgactggatt
     3781 tgcaatataa attgccgccc cttggaccgg gattatgtga gacaggaccc cggccagcac
     3841 aacacatttg ataaggactg gattcagttc gagcatgggt tggaagagat tgggcgtgac
     3901 gagtctcaaa aatgggtgtt gtttaaacat ccgacaaacg tcaaacgaaa ttgggagcag
     3961 gacatttcac gtcaatcgta ctgtcctgca gtatcgctct ccagcttaac ctgctacctt
     4021 tcctcatgag ccttcttcgg aagcacatat tgatatcgca agacagcggg acagcacctg
     4081 cgcatttttc gctaaaggaa tgttggaata acgtcaattc ggagaaaaaa gatttcaact
     4141 gaggtgcttt gttttgctct gctcaagaat gatttctctg cttgtgaggt acatttgcat
     4201 gtgatagatt cttcgcgcgc ttgcggcagt caatcgatct ttatctcgag atgaaagagg
     4261 atgggctgct tgcgttagct cattacagga aaaatgaaaa ccaggcagta aggataaagt
     4321 gactcgtttc gaaccagccc gttttacccg ggaggccgct gacgtcctct cgtctattct
     4381 tgataattcc cagtatcaat gcgcgactcg cctgccccag tcgggaggaa accgctggta
     4441 ttccgaacct gttcgactcg caaaatttgc cacaggggga gtgaactgct ggaaagaatc
     4501 accatggcaa ggcttatacc agaatctgct caagtgggag aagggctttt cacccgttgt
     4561 tttattcgat ctgttttttt tccacaaacc tgtttcagcg gatcggatta ctgccgttgt
     4621 cggtgccggt aacctcgaaa ttctcgtaga tgctggaatt ttgtcttcat cgcgtggcga
     4681 agtcagatca ctggttcgct gctaccccgt cggcaacatg tactttattt gcgatccgtc
     4741 cagggatcag tccgattttg tctatatcgg atgggattca gatttaatgg tcaatattgc
     4801 agcgaaatat tgcaggggcc gccgtttttc gcggttgctc gatctttgca cgggaagcgg
     4861 ggtacaagct ctttccctgt ctccgatctc agaggaagtt ttttgcgtgg atataaatcc
     4921 gagggcactt gcaatggccc aggcaaacgc tcgtctgaac aaactgccaa atgttaatgc
     4981 cgtccatagt gatcttttct caaatgtaac cgggcgcttc gactgcataa ccgcaaacac
     5041 gccctacgtc ccccatcccg agggggccca gcttcccatc ggcgggggcg atatcgggat
     5101 agaattcacg atccggcttc tccgcgaatt gcctgacaga ctcactgaaa agggaatttc
     5161 ggttatttac acgagtgacc cgatcgttcg gggcaagcgt cagttaatac cgcgagtcac
     5221 ggccgagctc ggccacttgc catttcgaat gatattgatt cctctcttca cgaacaatta
     5281 tccgatgact cggccgatgc aggagcatta cgacaggctc aacctaagcg ggtatgacga
     5341 ctgcatcctg ctcatcgagc acggcaagaa atacgaagtg gaacaccatc agcacgatgc
     5401 ccttcactac tatcgtacgc gaatggatgc ttggctcgac tggcgccgcc gtgcaatccg
     5461 gcacggctga taggcaagaa acgactttta gtcatcttgt tacgaatctt gctgtcccgt
     5521 ctgtcgcgca ggccagtccg tcgccggaaa aagtggatag cctcgaacgt acggctgagt
     5581 tctgataaag gctcgagata tcctctcatg gtccatccac ctcggctgta ctccgaatat
     5641 tctttcaatt gttgacgcaa aatggaaaaa ggccgaagca agtgctttgc ggcttctttc
     5701 ttgaaatgtc ctctaaaccc acgcatca
//
LOCUS       DASSHJ010000094        15364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_287583, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000094 DASSHJ010000000
VERSION     DASSHJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287583"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VFI43_03780"
     CDS             <1..1648
                     /locus_tag="VFI43_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7061279.1"
                     /translation="GYGAQSQRIWTAETDRTPAIAESISRDKDLTKELLRSCGIPVPE
                     GRPVTSPEDAWRAAEEIGMPVVVKPCDGNHGRGVFIELTSREEVESAYAVALAEGSGV
                     LVERYVPGTEHRLLVVGSGLVAASRGDSVSVIGDGKSTIRELIRDQINSDPRRGSTED
                     HPLNLVGMDGATIMEIGRQGFGVDSVPPAGLEVLIQRNGNHAFDVTDKVHPSIAAAAS
                     LAARVVGLDIAGIDLVARNISRPLAEQEGAIVEVNAGPGLLMHIKPAVGTPRPVGEAI
                     IEHLFPDSRQSRIPVVGISGSCGKTALAHLIARLLRISGRHTGLACSDGLYLDERLVH
                     KGDQAKWQAARNILMNRSVEAAVLENGVEGILQEGLAYDGCHVGILANLDLERHLGYF
                     GIETSEQVFDVLRTQVDVVLPEGAAILNACEPMFVKMAELCNGEVIYFSIDPDLSVIR
                     EHRLKGTQGVGTVCGKRAVILHSDEILLVDGPHEQRLARLSELSFSNPEDDVEQIENI
                     LAAVASVWALDMEPALVRTGIENFRGSRQILPNRPTGMAI"
     gene            1706..4270
                     /gene="cphA"
                     /locus_tag="VFI43_03785"
     CDS             1706..4270
                     /gene="cphA"
                     /locus_tag="VFI43_03785"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET7061280.1"
                     /translation="MKVSRIRALRGPNLWSRRTAIEAVVSCGSGYSAGALPDMLERLR
                     SRFPRINLNRRGRYETLTMAHALERVTLALQVEAGCSVTFSETAPALEDGIYRVVVEY
                     TEEAVGRLAVELGQALCRAAMENTPFDLGSAVSRLRELNEDIRLGPSTAAIVQAAVEH
                     GIPYRRLTDGSLVQFGWGSRQRRIQAAETDFTGAVSESIAQDKELTRMLLQAAGIPIP
                     PGRPVSGAEDAWAAACEIGLPVVVKPQDGNQGKGVGVNLATREQVQAAFAVAAGISGN
                     ILVERYITGQDYRMLVVGSKLVAAARREPPQVIGDGIHSVSQLVEQVNSDPRRGEGHS
                     NTLTKITLDGTALAHLATQGLTAESVPLAGKWIFLRNNANLSTGGAATDVTDEVHPEL
                     AERVVAAAKMVGLDICGVDVVCQDVSRPLEGQGAIIEINAAPGLRMHLQPSFGTGRKV
                     GEAIIAHMFAKGENARIPVVAVSGTNGKTTTVRMIANILNSQGFRVGMTSTDGVYVEG
                     HRIDTGDCSGPKSAKKVLLHPHVDAAVFETARGGLLREGLGFDQCDVAVVTNIGRGDH
                     LGLSYINTVEDLTRVKRVIVQNVTPGTGVAVLNADDPLVAGMAAHCRGKVTFFSRDAD
                     NPLIARHRAQQKRVIYFDQGAVVAWEAGAEYRIPISGIPATQNGVIVFQIENAMAAVG
                     AGWALGLEWDVVRAGLANFVGDTQSAPGRFNLFSHRGATLIADYGHNVDAIRALVRAI
                     DSMPAKRRSVVISGAGDRRDEDIRQQTEILGDAFDEVVLYQDQCQRGRADGEVLKLLR
                     QGLEKAKRARDVKEIRGEFVAIDAAVNNLKAGELCLILIDQVEEALGHITKRVTTG"
     gene            4599..5024
                     /locus_tag="VFI43_03790"
     CDS             4599..5024
                     /locus_tag="VFI43_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061281.1"
                     /translation="MQTHLRLTLFALLTLALHALFSQGLFAATPVLRCEIRQGGHAFT
                     EDFVPVTDPYAVDPKEINHQFLFKAVVMGNEENIDYIRIYTYYKEQHQVILLHEARYL
                     PPFRQGEPSPAALTGVNYLYAPVLEQEFQYGCVLLDGPS"
     gene            5315..5533
                     /locus_tag="VFI43_03795"
     CDS             5315..5533
                     /locus_tag="VFI43_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET7061282.1"
                     /translation="MSGDSVYKITEIIGTSKTSWEDAARNAVETAAKTLRDLRVAEIV
                     KLDLVVEDGKAVAYRARVKLSFKYEGHQ"
     gene            complement(5661..7919)
                     /gene="clpA"
                     /locus_tag="VFI43_03800"
     CDS             complement(5661..7919)
                     /gene="clpA"
                     /locus_tag="VFI43_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HET7061283.1"
                     /translation="MIAQELEVSLHMAFVESRQKRHEFITVEHLLLAMLDNPTAAEVL
                     RACSVDIEDLRRLLTEHVTENTPTVGGTGEVDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLHQKGVTRLDVVNYISHGISKVPQGSSAKTESEGDSDQ
                     ELGSGGALESYAVNLNSLALAGKIDPLIGREQELERLIQTLCRRRKNNPLLVGEAGVG
                     KTAIAEGLARRIIEHEVPDVLAHHQVYALDMGALLAGTKYRGDFEQRLKAVLKQLLDN
                     SNAILFIDEIHTLIGAGAASGGTLDASNLLKPILNTGQLKCIGATTYSEYRGIFEKDH
                     ALSRRFQKIDVPEPSVDETVAILRGLKARYEAHHGVKYTAVALSTAAELSARFINDRH
                     LPDKAIDVIDEAGAAQRILPKSRQRKVISKHEIEGIIAKIARIPAQNVSSDDRNTLKT
                     LDRDLKAVVFGQDKAINALTAAIKMARSGLGNPQKPVGSFLFSGPTGVGKTEVARQLA
                     YALGIQLHRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAIIKQPYSVLLLDEIE
                     KAHPDIFNILLQVMDHGTLTDNNGRKADFRNVVIIMTTNAGAEALTKSSMGFTKSAPM
                     GDEMADIKRMFTPEFRNRLDAIISFAPLDREIILRVVDKFLMQLEAQLHEKKVDAVFT
                     EKLREYLAQNGVDPLMGARPMARLIQDTIRSALADELLFGRLANGGRVTVDIGPDDKV
                     TLQFEEEAAATA"
     gene            complement(7921..8229)
                     /gene="clpS"
                     /locus_tag="VFI43_03805"
     CDS             complement(7921..8229)
                     /gene="clpS"
                     /locus_tag="VFI43_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET7061284.1"
                     /translation="MRTDNQKDIVLEAKKSKLKPPPMFKVVLLNDDFTPMDFVVAVLE
                     TFFSMNREQATKIMLKVHMDGAGVCGVYTSDIATAKVEQVVAFARKHEHPLRCVMEET
                     "
     gene            8521..8724
                     /locus_tag="VFI43_03810"
     CDS             8521..8724
                     /locus_tag="VFI43_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7061285.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGSEDLFAHFSAINMSGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQSV"
     gene            complement(8832..10085)
                     /gene="icd"
                     /locus_tag="VFI43_03815"
     CDS             complement(8832..10085)
                     /gene="icd"
                     /locus_tag="VFI43_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET7061286.1"
                     /translation="MYQHIKIPAEGTKIRIKQDLSLDVPDDPVIPFIEGDGIGIDITP
                     VMKKVVDTAVERSYGGKRKVCWMEIYAGEKANRVYGHEVWLPTETLEAAKEYVISIKG
                     PLTTPVGGGIRSINVALRQDLDLYVCLRPVRYFQGVPSPVKHPEKTDMVIFRENSEDI
                     YAGIEWEAGSEKAKKIINFLVNDMGVTKIRFPETSGIGIKPVSREGTERLVRRAIQYA
                     IDNKRRSVTLVHKGNIMKFTEGAFKNWGYALAKREFGATLLDGGPWMKLPAAHGGIIV
                     KDVIADAFLQQILLRPEEYDVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSIAIF
                     EATHGTAPKYAGKDRVNPGSIILSAEMMLRHMGWDEAASLIIGAMEKTILDKIVTYDF
                     ARQMTGAHEVSCSGFGQALVEHMGA"
     gene            10430..10876
                     /locus_tag="VFI43_03820"
     CDS             10430..10876
                     /locus_tag="VFI43_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7061287.1"
                     /translation="MIWKPNVTVAAIVEKDGKYLLVEEQTSHGILFNQPAGHLEPGES
                     IIQGVIRETLEETGYVFIPEAALGIYRWHSPSDDITFLRFAFIGAVIDHDPDRALDAG
                     ILRAGWFCVDEIRAMAYSHRSPLVMRCIEDHLAGRRYPLEIFTHLD"
     gene            10880..11962
                     /gene="mnmA"
                     /locus_tag="VFI43_03825"
     CDS             10880..11962
                     /gene="mnmA"
                     /locus_tag="VFI43_03825"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7061288.1"
                     /translation="MKKRRVVVGMSGGVDSAVAALLLKQQGYDVLGLFMKNWEDDDTD
                     EYCSSRQDLIDAASVADIIGISLEAVNFSAEYKDRVFSHFLAEYKAGRTPNPDVLCNA
                     EIKFKAFLEHAVELGAENIATGHYAQVRNTRGTLQLLKAEDGTKDQSYFLYRLNQAQL
                     SKTLFPIGHLYKREVRRIAKEHGLPNFSKKDSTGICFIGERPFREFLGRYLTDQPGEI
                     RTSDNTVMGQHKGLMYYTIGQRQGLGIGGTRDGDGEPWFVSSKDLAANVLTVVQGHQH
                     PDLFRSSLVATDLTWISGREPRCNWVYAAKIRYRQPDAPCALVRIDPDECEIEFAEPQ
                     WAIAPGQSVVVYESKVCLGGGVIKES"
     gene            complement(11996..12604)
                     /locus_tag="VFI43_03830"
     CDS             complement(11996..12604)
                     /locus_tag="VFI43_03830"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET7061289.1"
                     /translation="MKLIGSLTSPYVRKTRVVLAEKHIGYNFELEGSWNPETHVPEYN
                     PLGKVPVLIMEDGTSLFDSRVIVEYLDTVSPVSRLIPESNRRRIMVKRWEALADGICD
                     AAAAIFLERKRPEAQQSQEWISRQCKKINLGLESAARELSDKKWCDGNVYTLADVALG
                     CALGYLAFRFPEIDWPRDFPNLADFHERLEKRPSFMETAPQA"
     gene            12791..14176
                     /gene="purB"
                     /locus_tag="VFI43_03835"
     CDS             12791..14176
                     /gene="purB"
                     /locus_tag="VFI43_03835"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7061290.1"
                     /translation="MDYSPLTALSPLDGRYHTKVSALRPYFSEFGLIRYRVQIEIEWL
                     KALVLASGITQVPRLSDATISQLDGLLANFSEADADAIKGIESRTNHDVKAVEYWLRE
                     SLKSNVEMDAARSFIHFACTSEDINNLSHGLMFLHSRKQVMLPALDKIIRMLMATAND
                     LSDVAMLARTHGQPATPTTMGKEIANFVYRLERARKSVAGAPILGKINGAVGNYNAHL
                     AAYPAFEWEKFGRIFVENLGLQFNPYTTQIEPHDAMAELFDAYARINTILLDLDRDIW
                     GYIALGYFKQKVREGEVGSSTMPHKVNPIDFENSEGNLGIANALLRHLSEKLPVSRWQ
                     RDLTDSTVLRNVGVALGHTLLAYDSCSRGLGKLEINASRLGEDLENAWEVLAEPIQTV
                     MRRHGIADSYEQLKNLTRGKGGITREILHEFIGQLALPEAEKQRLYEMTPRSYTGNAA
                     ALAHRIGTESR"
     gene            14345..14905
                     /gene="grpE"
                     /locus_tag="VFI43_03840"
     CDS             14345..14905
                     /gene="grpE"
                     /locus_tag="VFI43_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7061291.1"
                     /translation="MTEKNPPQPDQPGDQAPHETTGAQANREAPDTMPSLEQLLKKAE
                     LDAEEHRDAWLRAKADAENIRKRAQIDIANAHKYAIENFSVELLPVMDSLDAALAVKN
                     ATVENFKSGMELTHKQLATAFEKFNIKVIDPQGEKFDPHLHQAIATVDSDLPPNTVVD
                     VMQKGYVLNDRVIRPAMVTVSKAKEA"
     gene            15004..>15364
                     /locus_tag="VFI43_03845"
     CDS             15004..>15364
                     /locus_tag="VFI43_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7061292.1"
                     /translation="MAKIIGIDLGTTNSCVAVMENGKPKVIENSEGARTTPSIVAYTE
                     DGEILVGASAKRQAVTNPKNTLFAVKRLIGRRFNEDMVQRDIKMVPYHIIKADNNDAW
                     IEVRGKKLAPPEVSAQVL"
BASE COUNT         3577 a         4042 c         4209 g         3536 t
ORIGIN      
        1 cggttacggg gcacaaagcc agcggatctg gacggccgaa acggatcgta cgcccgccat
       61 cgccgaaagc atctctcgcg acaaggatct gaccaaggag ttgctacggt cttgcgggat
      121 tcccgttccg gaaggacgac ccgtaacgag cccggaagat gcctggcgag ctgcggaaga
      181 gattgggatg ccggtcgtag tcaagccatg cgatgggaac cacggacggg gagtcttcat
      241 cgaactgact tcgcgggaag aggttgaatc cgcctacgcg gttgcattgg ccgaggggag
      301 cggagtcctg gtcgagcgct acgtcccggg caccgagcat cgtttgctgg tggtaggaag
      361 cggccttgtt gccgctagca ggggcgactc cgtatcggtc attggggacg gaaaatcgac
      421 tatccgcgaa ttgatccggg accagatcaa ttccgacccg cggcgcggct caaccgaaga
      481 tcatccactc aacctggtgg gcatggacgg ggcgaccatc atggaaattg gccgtcaggg
      541 attcggggta gattcggttc ctccagcggg actcgaagtt ctcatccagc gtaacgggaa
      601 ccatgcattc gatgttacgg acaaggtaca tccgagtatc gcggctgccg catcgctagc
      661 cgcgcgggtt gtaggactgg atatcgccgg gatcgatctg gtggcacgga atatttcacg
      721 accacttgcg gagcaggagg gggctatcgt tgaagtaaac gcaggaccgg gactgctgat
      781 gcacattaaa cctgctgtcg gcacccctcg tccggtgggt gaagcaatca tcgaacatct
      841 tttccctgat tctcgccaaa gccgtatccc tgttgtcggt atttcgggaa gctgtggcaa
      901 aaccgcgctg gcgcacctta ttgcgcggct gctcaggatt tcgggcaggc ataccggcct
      961 ggcctgtagc gatggccttt atctggatga aaggctggtt cacaagggag accaggcgaa
     1021 atggcaggca gccaggaaca tcctgatgaa tcgttcggtt gaagcggccg tcctggaaaa
     1081 tggggttgag ggtatccttc aggaagggct tgcctacgac ggctgtcatg tgggaatcct
     1141 tgcaaatctc gacctggagc ggcatctcgg ctattttggg atcgaaactt ccgagcaggt
     1201 cttcgatgtg ttgcgtaccc aggttgatgt tgtgctacct gagggagcgg cgatcctcaa
     1261 cgcatgcgaa cccatgttcg tgaagatggc tgaactatgc aatggggaag tcatctattt
     1321 cagcattgat cctgatctgt cggtcatcag ggagcatcgt ctcaaaggca ctcaaggtgt
     1381 gggtacggtt tgcggtaagc gggctgtgat ccttcacagt gatgaaatcc ttcttgtgga
     1441 tggaccgcac gaacagcggc tggcaaggct cagcgagctg tcattttcca atcccgaaga
     1501 cgacgtcgag cagatcgaga acattctcgc ggctgtggct tctgtctggg cgcttgatat
     1561 ggagccggca ctcgtaagaa ctggcattga aaatttccgt ggatcgagac agattttgcc
     1621 gaatagaccg accggcatgg ccatataaga aggaatcaaa ttggcaacac ttcatcgcga
     1681 agaaccacat ccaggagaat ggttaatgaa agtatcccgt atccgtgcct tgcgtggccc
     1741 taatctatgg agccgcagga cggcaataga agcggttgtt tcgtgcggga gcggatattc
     1801 tgccggagct cttccagata tgctggaacg attgcggtcg cgatttcccc gaattaatct
     1861 gaatcgccgc ggtcgctatg aaaccctgac catggcgcat gcgcttgaaa gggtgactct
     1921 ggcgttgcaa gtggaggcag gatgttccgt tactttcagc gaaaccgcgc ctgcactgga
     1981 ggacgggatt tacagagtcg ttgtggaata taccgaagaa gctgtcggcc ggctagccgt
     2041 cgagttgggg caagcgttgt gccgggcagc tatggaaaat actcccttcg accttggcag
     2101 tgccgtgagc cgcttgcgcg agctgaacga ggatatacgt ctgggaccga gtacagcggc
     2161 aattgttcag gctgctgttg aacatggtat cccctatcgt cggctgacgg atggcagtct
     2221 cgttcagttc gggtggggca gcaggcagcg ccgcatccag gctgccgaga cagactttac
     2281 cggtgcggtt tccgaatcga ttgcgcagga caaagaattg acgaggatgc tcctgcaggc
     2341 ggcggggatt ccaatccctc ccgggcggcc cgtatcgggt gccgaggacg cctgggcggc
     2401 tgcgtgcgaa atcgggcttc cagtcgttgt caagccgcag gacgggaatc agggcaaggg
     2461 cgttggggtc aatctggcaa cccgcgagca ggttcaggcc gcttttgcag tcgcggccgg
     2521 gatcagcgga aatatcctgg ttgagcgata tattaccggg caggactatc gcatgctggt
     2581 cgtgggcagc aaactggtcg ccgcggcacg ccgggaacca ccacaggtca tcggcgacgg
     2641 gatacacagc gtcagccaat tggtcgagca ggtcaacagc gaccctcggc gcggcgaggg
     2701 gcactcaaac accttgacca aaatcacttt ggacgggacc gcgctcgccc atctggcaac
     2761 gcaagggttg accgccgaat ccgtccctct cgccggcaaa tggatctttt tgagaaacaa
     2821 tgccaatctt tccacggggg gggcggccac tgacgttacg gatgaggttc atcccgagct
     2881 ggcggagcgg gtggtagcgg cagcaaaaat ggtcggactg gatatctgcg gcgtcgatgt
     2941 cgtttgccag gatgtttcca ggccgctgga ggggcagggg gcgatcatcg agatcaatgc
     3001 ggcacccggt ctgcggatgc atctgcagcc ctcgtttggg acagggcgca aggtcgggga
     3061 ggcaattatc gcgcatatgt tcgccaaagg ggagaatgcg cgcatacctg ttgtggcggt
     3121 gagcggcacc aatggaaaaa caacgactgt ccggatgatc gccaatattc tcaactccca
     3181 gggcttccgc gtgggaatga ccagtaccga tggcgtatac gtggaaggcc accggattga
     3241 tacaggggat tgcagcggtc ccaagagcgc aaaaaaagtg ctgcttcacc cgcacgtgga
     3301 cgccgcagtg tttgaaactg cgcgcggtgg attattacgc gagggcttgg gtttcgacca
     3361 gtgtgacgtc gcggtggtga cgaatattgg aagaggcgat catctcggtc tttcatacat
     3421 caataccgtg gaagatttga cacgcgtaaa acgggtcatt gtgcagaacg ttacgccagg
     3481 gacaggagtt gccgtactca atgccgacga tcctctggtg gcagggatgg cggctcattg
     3541 ccgagggaag gtgactttct tttcgagaga cgcggacaat cccctcattg ccagacatcg
     3601 tgcacaacaa aagcgcgtga tctacttcga tcagggggca gtcgttgcgt gggaagccgg
     3661 cgctgaatac cgcattccca ttagcgggat tcccgccacc caaaatggcg tcattgtctt
     3721 ccagatcgaa aatgcgatgg ctgccgtcgg cgctggctgg gctcttgggc tggaatggga
     3781 tgtcgtccgt gccggcttgg ccaattttgt cggcgacacg caatccgccc cggggcgctt
     3841 taatctgttc agccatcgtg gtgctaccct gatcgcggat tacggtcaca atgtcgatgc
     3901 aatccgggct ctcgtccgtg ccattgacag catgccagcc aagcgacggt cagttgtaat
     3961 cagcggggcc ggtgacaggc gcgatgaaga tatccggcaa cagacggaaa tacttgggga
     4021 cgcattcgac gaggtcgtgc tgtatcaaga tcaatgccag agaggccgcg ccgatggcga
     4081 ggtgctgaaa ttgctgaggc agggtcttga aaaagcaaag cgtgcccgcg atgtcaagga
     4141 aatccgtggg gagttcgtcg caattgacgc ggctgtcaat aatcttaaag ccggagaact
     4201 ttgcctgatc cttatagacc aggtggaaga agcgctgggg cacattacga agcgggtgac
     4261 gacaggctga tctgctgtgc ggcttggcgg cgaagcttga caggcggacg ctttctcttc
     4321 cgctctatgg gaaacaggtc agagtgctgt caggatttca cggcgggtga gagcaaaacc
     4381 gtctggatga ccaacgagct atgcttccct ctctcgatct tcaggtattc caggccttgt
     4441 tacacaccca ttaagtgcta tgataccgcc tcctgagctg gattttcaga ttttctttcg
     4501 atctcacaca atcctgaccc agctttccga gcagttagcg ccggtcgctt tactgatgga
     4561 ttttagggat ggaaccggta tcggattagc tgaacgttat gcaaacgcat ttacgcctga
     4621 cgctattcgc cttgctgact ctggcgcttc acgcgctgtt ttcccaaggc ctgtttgctg
     4681 cgaccccggt gctcaggtgc gaaattcgcc aagggggtca cgcattcacg gaagattttg
     4741 tgcccgtgac tgatccctat gcagttgatc ccaaagagat caaccaccaa ttcctgttca
     4801 aggcggtggt aatgggtaac gaagagaata tcgattacat aagaatctac acttattaca
     4861 aggagcaaca tcaggttatc ctgctccacg aagccaggta tctacccccc tttcggcaag
     4921 gtgagccgtc gcctgctgcc ctgaccggcg tgaactatct atacgcacct gtactggagc
     4981 aggagtttca atacggctgc gtgcttctcg acggcccatc gtaacactgt tcaaacattt
     5041 gggtttgatg ctcggcggac acagcatatc gttgtgcttg ctgaagcggc aggaatgctt
     5101 cggtcctcgc gggaccgaag gaaaatcgtt gattgcaatt tgcccaggtt catctaaact
     5161 gaagccggta gcgcttgcaa agcggacatt tatagggagt aagagagatt gataagcgga
     5221 gattgataca gcgcagcgtg aaatcgcgcg aaccttccct agtgacttgc gcaccagttt
     5281 gtttgcctct catttataag aaggaggaaa gataatgtca ggagatagtg tctacaagat
     5341 taccgagatc atcggcacta gcaagacttc ctgggaagat gctgccagaa atgcggtcga
     5401 gaccgcggcg aaaactttga gggatctcag ggttgcagaa atcgtgaagc ttgacctggt
     5461 ggttgaagat gggaaagcag tcgcgtatcg cgcgcgcgta aagctgtctt tcaaatatga
     5521 agggcaccaa tagatagagg cattgatggc cggcagacac gggcacaggt accgtatgta
     5581 gaggccagcg ccaggaaatt tctccgcgca cttcccaaaa aaaggcgcaa actttgcgcc
     5641 tttttttacg agcggcctct tcaggcagta gcggccgctt cctcttcaaa ctgaagcgta
     5701 accttgtcgt cagggccaat atctaccgtc acccttccgc cattcgccaa ccggccgaac
     5761 agaagctcat ctgcaagggc actgcgaatg gtatcctgaa tgagtcgggc catcggtcgt
     5821 gctcccataa gcgggtcaac cccattctgt gcgaggtatt cccttagttt ctcggtaaat
     5881 accgcatcca ctttcttttc gtgcaactgc gcttcaagct gcatcaggaa cttgtcgacc
     5941 acccgtaaga tgatctccct gtcgagcggc gcaaatgaaa taatcgcatc cagccgattg
     6001 cggaattcgg gcgtgaacat gcgtttgata tccgccatct catctcccat tggagctgat
     6061 ttggtaaaac ccatcgagct tttcgtaagc gcctcagctc cggcattcgt ggtcatgatt
     6121 ataacgacat tacgaaaatc tgccttgcgg ccattgttgt ccgtcaatgt cccatgatcc
     6181 attacctgga ggagaatatt gaagatatcc gggtgcgcct tttcaatttc atccagtaaa
     6241 agcactgaat agggctgctt gataatcgcc tcggtcagca agcctccctg atcgaagcct
     6301 acatagccgg gaggcgcgcc gatcaggcgg gataccgcgt gccgctccat gtattccgac
     6361 atgtcgaacc gatgcagctg aatacccagc gcgtatgcaa gttgtcttgc cacctccgtt
     6421 tttccgacgc ctgtcggtcc ggagaacagg aaggatccaa cgggcttctg aggattgccc
     6481 aaaccgcttc tcgccatttt gatcgccgcc gtaagtgcat tgatcgcctt gtcctggccg
     6541 aataccaccg ccttcagatc acggtccagt gtcttcagcg tgtttcgatc gtcgctggac
     6601 acattttgcg ctgggatgcg cgcgatcttc gcgataattc cttctatttc gtgtttgctg
     6661 atgaccttac gctggcgtga ttttgggagg atgcgctgtg cagccccggc ttcgtctatc
     6721 acgtcaattg ctttatcggg caaatgacgg tcgttgatga agcgtgcaga caactcggcc
     6781 gcagtactga gggccaccgc agtgtattta acgccatgat gagcttcgta gcgcgctttc
     6841 aaacctctca ggatcgccac cgtttcgtcc acgctcggct caggcacatc gatcttttga
     6901 aagcggcgcg agagggcgtg atccttttcg aagattccgc ggtattcgct atatgtcgtg
     6961 gcgccaatgc atttcagctg gccagtattc aatatgggct tcagcaagtt ggaagcatcc
     7021 agcgtgccac cagaggcagc ccctgctcca atgagcgtat gtatctcatc gatgaaaagg
     7081 atggcattgg aattgtccag caactgcttc agcaccgcct tcaagcgctg ttcaaaatca
     7141 ccccggtact tcgtccctgc aagcagcgca cccatatcca gtgcatagac ctgatggtgc
     7201 gccagcacgt ctggcacttc atgttcgatg atgcgtcgcg cgaggccttc ggcaatggct
     7261 gttttgccaa ctcccgcttc cccgaccagc agggggttat tcttgcgccg ccggcacagc
     7321 gtttgtatca aacgctccag ttcctgctcc ctgccaataa ggggatcgat cttccctgca
     7381 agcgccagcg agttcaggtt tacggcgtag ctctccagcg ctcctccgga acccagttcc
     7441 tgatcggaat caccctcgct ttctgtttta gcgctgcttc cctggggtac cttgctgatg
     7501 ccatgggaaa tatagttcac gacatccagg cgggttacac ctttctgatg cagaaagtac
     7561 actgcatggg aatccttttc gccgaatatg gcgaccagca cgttggcgcc cgtaacctct
     7621 tttttccccg aggattggac atgcagaatg gcgcgctgga tcacgcgttg aaaaccaagt
     7681 gtaggctgcg tatcgacttc gccggtacct cctaccgtcg gcgtattttc cgttacgtgt
     7741 tctgtcagaa gtctccttaa atcctctatg tcaactgagc aggcgcgcag cacctccgcg
     7801 gcggtgggat tatcgagcat cgccaacagc aaatgctcca cggtaataaa ctcgtggcgt
     7861 ttctgccggg actcgacaaa agccatatgc aaacttactt ccagttcttg cgcaatcatt
     7921 tcaggtttcc tccatcacgc accttagcgg atgttcatgc tttctagcaa acgcaacgac
     7981 ctgctcaacc ttggcagtgg cgatatcact ggtataaacc ccacatacgc cagcaccatc
     8041 catgtgaact ttgagcataa ttttcgtggc ctgttcgcga ttcatcgaaa aaaaagtctc
     8101 taacacggca acgacaaaat ccatgggggt aaaatcatcg ttgagcaaga cgactttgaa
     8161 catcggagga ggctttagct tgctcttttt agcctccagt acgatatctt tctgattatc
     8221 cgtccgcatt tccttaccct gccttcaact tctgttgcgt aaatccaatc agaacaattc
     8281 ctgcctctta ctgatatatt tgatgattgg aggcaaatat tcaagtagtt tgggaaaatt
     8341 attcttttcc aggcggccaa aataagtagg tttactactt gacttgaaaa ccggatttga
     8401 gtaaaacatc cttggcgttt ggcttcaagt tctctgcagt accggttata gccgttttgc
     8461 ggtcttgaaa ctcaaacagt gttcgggttt ccggcccggt tttttattta ggaaacaaaa
     8521 atggcgacag gtacagtgaa gtggttcaac gattcaaagg gttttggctt tattaccccg
     8581 gatgacggca gcgaagattt gtttgcacat ttctccgcta tcaacatgtc agggttcaaa
     8641 actctcaaag aaggtcagaa ggtaagtttt gaggtgacgc agggcccaaa aggcaagcag
     8701 gcatcgaaca tccagtcggt ttgattatcg cttgctgagg cggacacccg aatcagcttg
     8761 taatcacaga attatcgacg ccccttgccg gaaattccgg caaggggatt tttttgtctc
     8821 cacttcaaat atcaggcgcc catgtgttca accagcgcct gaccaaatcc ggaacatgat
     8881 acctcatgcg cgccggtcat ctgccgcgcg aagtcatagg tcacgatttt atcgaggatg
     8941 gttttctcca tcgcgccaat aatgagactg gccgcttcgt cccatcccat gtgcctcagc
     9001 atcatttccg ccgataagat aattgaaccg gggttaaccc gatcctttcc tgcatacttc
     9061 ggcgcagtgc cgtgcgttgc ctcgaagatg gcaatggaat cactaaggtt tgctcctggc
     9121 gcaattccaa tgcctccaac ttgcgcggcc aaggcgtccg agatgtaatc gccattcaaa
     9181 ttgagtgtcg cgatgacatc gtattcctca gggcgcaaca atatttgctg cagaaacgcg
     9241 tcagcgatta cgtccttgac aatgattccg ccgtgcgccg caggcagctt catccaggga
     9301 ccgccatcca gcagcgtagc tccaaactct cgtttcgcca atgcgtatcc ccagttcttg
     9361 aaagcgccct cggtaaattt catgatattt cccttgtgca cgagggtcac ggagcgccgc
     9421 ttgttgtcga tcgcatactg gatcgcccgg cgcacaagcc tttccgtacc ctccctcgaa
     9481 acaggcttga ttccgatgcc cgacgtctca ggaaaacgaa tttttgtcac tcccatgtca
     9541 ttcacaagga aattaatgat tttttttgcc ttttccgaac ctgcttccca ttcgatcccg
     9601 gcataaatat cttccgaatt ttcacgaaaa atgaccatat cggttttttc aggatgcttc
     9661 actggactgg ggactccctg aaagtagcgc acagggcgca aacagacgta gagatcaagg
     9721 tcctggcgca gggcgacatt gatggagcgt attcctcccc cgaccggggt ggtaagcggc
     9781 ccttttattg aaatgacata ttctttcgcc gcctccagtg tttcggtagg cagccagacc
     9841 tcatggccat agacgcgatt cgccttttcg ccggcgtaaa tttccatcca gcataccttc
     9901 cgcttgcccc cataagagcg ctccaccgcc gtatccacca cttttttcat gaccggggtg
     9961 atatcgattc cgataccatc gccttcaatg aaaggaataa cgggatcatc aggcacgtcc
    10021 agggaaagat cctgttttat tctgattttt gtgccttcgg ccggtatttt gatatgttga
    10081 tacatgtaag ctccaaattg agtttggccg ttagcctgaa acacggccgg actttttgtt
    10141 atttacccaa atgctatttc ggggggtcgt cgtaattaaa aaacctgcat attttacccc
    10201 cggtttattt acccccggtt taaattgcga aggcgacgat ggatgctttc gcttccatcc
    10261 atcaggttat ctgtttattt gcatcatttt ttaatggccc agtttatttc tccagcaagg
    10321 gatatagaat tcgactcttg cagccgggcc tgcgttcgag ctgcgcaaat ataaaaagcc
    10381 aggtccacag ctataaaaag ccaagtccac accgatggtt caagggaaaa tgatctggaa
    10441 gccgaatgtc accgttgccg cgattgtgga gaaagacgga aaatatttac tggttgagga
    10501 gcaaacgagt cacggaatac tattcaacca acctgccggg catctcgaac cgggcgagtc
    10561 gatcatacaa ggtgtgatcc gggagacgtt ggaagagacg ggatatgtgt tcattccgga
    10621 agcggctctg ggcatctacc gctggcattc tccatccgac gacattactt ttcttcgctt
    10681 cgcgttcata ggtgcggtga tcgaccacga tccggacagg gcgctggatg cagggatatt
    10741 gcgtgccgga tggttctgcg tggatgaaat ccgggcaatg gcttactctc accggagccc
    10801 gctcgtgatg cggtgcatcg aagatcatct cgcgggcagg cgctaccctc tcgaaatatt
    10861 cacccatctg gattaatcaa tgaaaaagcg gcgcgtggtg gtcgggatgt cggggggcgt
    10921 cgattcagcc gtcgctgcat tgcttttaaa acagcagggg tacgacgtcc tcggcctctt
    10981 catgaagaac tgggaagatg acgacactga tgaatactgc tcgtcccgcc aggacttgat
    11041 tgatgctgct tcagtcgctg acatcatcgg tatctcgctg gaggcggtca acttctcggc
    11101 ggaatacaag gaccgcgtat tcagccattt cctcgccgag tataaggccg gacgtacgcc
    11161 caaccccgac gtgctgtgca atgccgagat caagttcaag gcttttctcg agcacgcggt
    11221 tgagctcggc gcggagaaca ttgcgacggg acattatgcc caggtaagga atacgcgcgg
    11281 aacgctccag ttgctgaagg cggaggacgg cacgaaagac cagagctact ttctttaccg
    11341 cttgaatcaa gcgcaattat ccaaaacctt gtttccaatc ggccatctct acaagcggga
    11401 ggttcgcagg attgctaaag agcacgggct gccgaatttc tccaaaaaag acagcacagg
    11461 aatatgcttt ataggcgagc ggccgttccg ggagtttctt ggccggtatt taacggatca
    11521 gccgggagaa ataaggacat ccgacaacac agtcatgggc cagcataaag gcttgatgta
    11581 ttacacgatc gggcagcggc aggggttggg gatcggcggc accagggacg gagatggcga
    11641 accgtggttt gtatcgagca aggatctcgc ggcgaacgtc ctgacagtcg tccaaggtca
    11701 tcaacatcct gacctgttca ggtcctctct cgtggcgacc gatctgacat ggataagtgg
    11761 ccgagagccg cgctgtaatt gggtttacgc ggcaaaaata cgctatcgtc aacccgatgc
    11821 tccatgcgcg ctcgtacgaa tcgaccccga cgaatgcgag atcgagtttg ccgagccgca
    11881 atgggcgatt gcgccaggac aatcggttgt ggtttacgag agcaaagtat gccttggggg
    11941 cggcgttatt aaggaaagct gaaaaacacg tccgttgctg ctccgtttag ggtaatcatg
    12001 cttgaggggc ggtttccatg aaggagggac gtttttcgag cctttcgtga aaatccgcca
    12061 gattgggaaa atcccggggc caatcaattt caggaaagcg aaaagcaaga tagccgaggg
    12121 cacagcccag cgcaacatca gcgagtgtgt agacattacc gtcgcaccat tttttgtcgc
    12181 tcaattcccg cgcggcgctt tccaacccaa ggttgatttt tttgcactgc cgcgagatcc
    12241 attcctggct ttgctgggcc tctgggcgtt tccgttccag gaatatcgcg gctgcggcgt
    12301 cacagatacc atccgccaga gcctcccagc gcttgaccat gatgcgtcgg cggtttgatt
    12361 ccgggatgag acgggaaacg gggctcaccg tatccaggta ctcgacaata acgcgcgaat
    12421 caaacaggga ggttccatct tccataatga gcacgggtac ctttcccaag gggttgtatt
    12481 caggaacatg cgtctccgga ttccacgatc cctccagttc gaagttataa cctatatgtt
    12541 tttccgccag gacgacacgc gttttacgta catatggact ggtgagggat ccgatgagct
    12601 tcatgacaac ctctggggaa acaatcgtca gcaggggatt gcacggcaat aagccatccc
    12661 cccaatcgcg gcgcaattca atggtcccct ggcattatat cggctttccc acgacggtga
    12721 caggaagtgg gcaggccatc ccggctggtt acgtatgtta aaatggcttt ccggataccc
    12781 ggatcaaaac atggattatt ctcctcttac tgccttatct cctctcgacg ggcgttacca
    12841 cactaaagtc tcggctctca gaccttattt cagcgaattc ggactcattc gctacagggt
    12901 tcaaatcgaa atcgaatggc tcaaggcgct cgtcctcgcg tccggcatca cgcaagttcc
    12961 gcggctttcc gacgccacta tcagtcagct tgacggtctc ctggcaaatt tttccgaagc
    13021 ggacgccgat gcaatcaagg gcatagaatc ccgcacaaac catgacgtca aagccgtgga
    13081 atactggttg cgggaaagct tgaaaagcaa cgtcgagatg gacgcggcga ggtcgttcat
    13141 ccattttgca tgtacttccg aagatatcaa caacttgtcc cacggcttga tgtttttgca
    13201 tagccggaaa caggtcatgt tacccgcact ggataaaatc attcgcatgc ttatggcaac
    13261 ggcaaatgat ctgtctgacg tagccatgct cgcccgtacg cacgggcagc cggctacccc
    13321 tacaacgatg ggcaaggaga tcgccaactt cgtctaccga ttggagagag cacggaagtc
    13381 ggtggcgggt gcaccgattc ttggcaagat caatggcgcg gtaggcaatt acaacgcgca
    13441 tctcgcagct taccctgcat tcgaatggga gaaatttggg cgtatcttcg tcgaaaatct
    13501 gggcctgcaa ttcaatccct acactacgca aatagagccg catgatgcaa tggcggagtt
    13561 attcgatgcc tacgcccgca ttaacaccat cctgctggac ctcgaccggg atatctgggg
    13621 ctatattgct ttaggctact tcaagcaaaa ggtccgggaa ggcgaagtcg gctcctcaac
    13681 aatgccccac aaagtcaacc cgatcgattt tgaaaactcc gaaggcaacc ttggtattgc
    13741 gaatgcgctt ttgcgccatc tgagcgaaaa actgccggtg tcgcgatggc agcgcgatct
    13801 taccgattcg actgtgctgc gaaacgtcgg agtggccttg ggtcacactc tgcttgccta
    13861 tgattcatgc agcagggggc tgggtaaact ggaaatcaat gcttcccgtt tgggtgaaga
    13921 cctggagaac gcctgggaag ttctggctga accgatccaa accgtgatgc gacgtcatgg
    13981 catagctgat tcctacgagc agttgaagaa tctcacccgg ggaaagggag gaattaccag
    14041 ggaaatcctg cacgaattca tcggacagtt ggctcttccg gaagcggaaa agcaacggct
    14101 atacgagatg actcctcgaa gctataccgg gaatgccgcc gcgctggctc atagaatcgg
    14161 tacggaaagc cggtaaaggt ctaaactcta aattcctctg cagcattccc cctttcacaa
    14221 ccgaagtggc atattcggct agcagacagg taaagttcgc caacgcgcca attgttgctg
    14281 ggtgagcttg aaatcaggga aaggatcccc atatgccgct ggataaaaac aggagacgcg
    14341 acccatgaca gagaaaaacc cccctcaacc ggatcagcct ggggaccagg cgccccacga
    14401 aacgactgga gcacaagcca accgagaggc tccagacact atgccgagcc tcgagcaact
    14461 gcttaaaaaa gccgagcttg acgctgaaga acatcgggat gcctggttgc gcgcgaaggc
    14521 ggatgccgag aacatccgta aacgtgccca gatagacatc gccaacgccc acaaatacgc
    14581 aatcgaaaac ttttccgttg aactgcttcc agtaatggat agcctggatg ccgcattggc
    14641 cgtaaagaat gcgacggtcg agaattttaa aagcggaatg gaactgaccc acaagcagct
    14701 cgccactgct tttgaaaaat tcaatatcaa ggtgatcgat cctcaggggg aaaaatttga
    14761 cccgcacttg catcaggcga tcgccaccgt cgattccgat cttcctccca ataccgtagt
    14821 cgacgtgatg cagaagggct atgttttgaa cgatcgggtg atcaggcccg cgatggtgac
    14881 tgtttcaaaa gccaaagaag cttgaatggc ttgaaatccg cggtacgacc cccacttttc
    14941 ctgatggaac ggcattttac gcaggcagat atttgacccc aaccaatcaa caaggattaa
    15001 atcatggcaa aaattattgg cattgacctt ggcaccacga attcatgcgt ggctgtcatg
    15061 gagaacggaa aacctaaagt catcgaaaat tccgagggag ctcgcaccac tccttccatt
    15121 gttgcctata cggaggatgg agaaattctg gttggggctt cagccaaacg tcaggcggtg
    15181 accaacccga aaaatacgtt gttcgcggtg aaacgcttga ttggccggcg attcaacgag
    15241 gatatggtgc agcgggacat caagatggtg ccctaccata tcatcaaggc cgacaacaat
    15301 gacgcctgga tcgaggttcg gggcaagaaa ctcgctccgc ccgaggtttc ggcgcaggtc
    15361 ttga
//
LOCUS       DASSHJ010000095         6013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_134118, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000095 DASSHJ010000000
VERSION     DASSHJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134118"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            517..2283
                     /locus_tag="VFI43_03850"
     CDS             517..2283
                     /locus_tag="VFI43_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET7061293.1"
                     /translation="MKENVSDFLVQRLSEWGVKRIFGFPGDGINGILGALNRAQDSID
                     FIQVRHEEMAAFMACAHAKFTGEVGVCLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQ
                     QKRAALGGNYQQEVDLISLFKDVAHEYVHMVSTPQQMRHIIDRSMRIAQVERNVCCMI
                     VPNDIQELHAEKPAREHGTIHSGIGYKEPRVVPRPEDLQRAADILNAGSKVAMLVGAG
                     ALHATAEVLRTAELLGAGIAKALLGKAVVPDDLPFVTGSIGLLGTRPSYDMMMQCDTL
                     LMVGSSFPYSEFLPEEGQALGVQIDIDGRMTSIRYPMKVNLIGDSAETLRQLIPLLKQ
                     KEDRTWRERIEGEVAKWWKTMEARAMESANPINPQRVFWELSPRLPDDCILTCDSGSS
                     ANWYARDVKVREGMMASLSGGLATMGPGVPYSIAAKFAHPHRTVIAMVGDGAMQMNGM
                     NELITIGKYWRRWSNPHLIVMVLNNRDLNQVTWEQRAMEGDPKFDASQDIPDFPYAAY
                     AELIGLRGIRVDRPEQIGDAWDSALNADRPTVIEAYTDPDVPPLPPHITFKQAKAYAS
                     AILHGDTDSPEIIKSSVKQIFA"
     gene            2293..3417
                     /locus_tag="VFI43_03855"
     CDS             2293..3417
                     /locus_tag="VFI43_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7061294.1"
                     /translation="MNANTVRGRNGSSSIPVSRVQVSAFSTSTATPESDGTLEWNATV
                     LVLVEVEAGGKTGIGFSYADTGTATLIHDTLAAVVTGRDALAIPDCWNAMIAAIRNLG
                     RPGIASMAVAAVDNALWDLKGHVLDLPLVSLLGQARPAVPIYGSGGFTSYSVAELQKQ
                     LGDWASSGIARVKMKIGREPGKDVARVRAAREAIGSGVELFVDANGGYSRKQALAMAF
                     SFSEFSVSWFEEPVPSDDLEGLRLMRDRAPPGMDISAGEYGYHATYFRRMLQARAVDV
                     LQADATRCAGVSGFMQAAALCHGFNLPLSSHCAPSIHAHPCCAATPVRHLEYFYDHVR
                     IEKMLFDGVLEPEDGLLRPDLTRPGFGLEFKRADAERYAV"
     gene            3460..>6013
                     /locus_tag="VFI43_03860"
     CDS             3460..>6013
                     /locus_tag="VFI43_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HET7061295.1"
                     /translation="MRRPTTPLLDAQALAAELKGDIQGEVRFDDGSRALYATDGSNYR
                     QVPIGVVIPKSIDDIIATVAACRRHGAPVLSRGGGTSLAGQCCNVAVVMDMSKYFNRI
                     LELDPEKKIARVEPGCVLDNLRDAAEQHHLTFAPDPSTHTHNTLGGMIGNNSCGVHSV
                     MGGKTVDNVIELDVLLYDGTRMKVGKTSDAELAEIIAAGGRRGEIYGRLKSMRDRLAD
                     QIRARYPKIPRRVSGYNLDDLLPENGFDVARALVGTESTCVVILEATLRLVYSPPGRS
                     LLVLGYPDVYSAGDHVPDILKFGPIGLEGIDDRLIADMKAIGLHPEDVHLLPPGGGWL
                     LAEFGGRDKSESDACANACMNALKQTKDPPTMKLFDNREEEQMIWKVRESGLGATAHV
                     PNKNITWEGWEDAAVPPEKVGAYLRDFRALLDRFHYGCDLYGHFGQGCIHTRIDFDLE
                     THDGIQIFHDFLSHAADLVVSYGGSLSGEHGDGQSKAEFLPKMFGNELVEAFGEYKSI
                     WDPDWKMNPGKIVDAYLPTENLRLGTAYDPPQPDTYFKFPGDKGNFGRVTLRCVGIGN
                     CRQQHSQTMCPSYKVTREEKHSTRGRARLLFETMRGEVIKDFWQSDEMRESLDLCLAC
                     KGCKGDCPVNVDMATYKAEFLSHYYEHRRRPRQAYAFGLIDRWARLAAYAPSVVNWFT
                     QTRGLSGLAKSIAGMPEQRAIPRFASETFLQWFRKRPLRNSGKPEVILWPDTFNNHFY
                     PQTAKAAVAVLEAAGFQVRVPVQHICCGRPFYDFGMLDRAKEQLRRILASMREDIDNG
                     VPIVGMEPACVAVFRDELVNLFPADEVAKRLNRQTYFFSEFLVRRAPGFSYPK"
BASE COUNT         1303 a         1682 c         1768 g         1260 t
ORIGIN      
        1 aataccagat cgctttctaa tagctggagt ccgtcagtct cagcgattca agctcaggca
       61 ggccttagtc ggcctgcctg aagtaatctt ccgcctcctc atcaatcctc tgcctctcgg
      121 gcccctatcc cgggagtttt ctttttccac gcccccgata tccctgtgtt ccgcgaaggg
      181 gatgacttac gcgtgtcttc gtccctgtcc tgtccccatc atgccttccc tacattgaaa
      241 aatgtgggta tctgagaaca ttgttcaaga aaacataagt gtcttaacgc acagaatatg
      301 cgactagacc gcgtactatt acccggtgct acatcctctc cccggatcgg ggagtcacgc
      361 tccggagagt gaagctggac gcgagacact ataagcccgc gaatactgac aagagggcgc
      421 acccgcaaac gcgggaatca gtctgaaaac cgcggaatga agcaagtcag tcctctggca
      481 tttgctcgct ctgcggatca acagagggga tgcgcgatga aggaaaacgt cagtgatttc
      541 ttggtgcagc gcttgtccga atggggcgtc aagcgtattt tcggctttcc tggtgatggt
      601 atcaatggaa tcctgggcgc gctcaaccgc gcccaggatt ccatcgattt tatccaggtg
      661 cggcacgagg agatggccgc cttcatggcg tgtgcccacg cgaaattcac gggcgaggtc
      721 ggcgtttgcc tggccacctc cggcccgggc gccattcatc tgctcaatgg gttatacgac
      781 gcgaagctcg accatcagcc ggtcgtcgcg atcgtcgggc agcagaagcg tgccgcgctg
      841 ggcgggaact accagcagga agtcgatctc atttccctgt tcaaggatgt cgcgcacgaa
      901 tatgtgcaca tggtttccac gccccagcag atgcgtcaca tcattgaccg ttcgatgcgc
      961 atcgcccaag tggaacgcaa cgtatgctgc atgatcgtgc ccaacgacat ccaggaactg
     1021 catgcggaaa agccggcgcg cgagcatgga acgatacatt ccgggattgg ctacaaggaa
     1081 ccccgggttg tgccgcgacc agaagatttg cagcgcgctg ccgacattct caacgccgga
     1141 tcgaaggtgg caatgctggt gggagcaggc gcgcttcacg ctacggccga ggtgctgaga
     1201 accgctgaac tcctgggcgc ggggatcgca aaggcgctgc tgggcaaggc cgttgttccg
     1261 gacgacctcc cgttcgtcac gggctctatt ggtttgctcg gcacgcggcc gagctacgac
     1321 atgatgatgc agtgcgacac gcttctcatg gtcggatcga gttttcccta ctctgaattt
     1381 ttacctgagg aaggccaggc gctcggcgtg caaatcgata tcgatggacg catgacgagt
     1441 atccgctacc cgatgaaagt aaatctcatc ggagacagtg ccgaaacgtt gagacaactc
     1501 atcccgctgc tgaagcaaaa agaagaccgc acctggcgag aaagaatcga gggtgaagtg
     1561 gcgaaatggt ggaaaaccat ggaagcccgc gcgatggagt ccgccaaccc tatcaacccc
     1621 caaagagtat tctgggaact ctccccgcgc cttccggatg actgtatcct cacctgtgac
     1681 tcaggctcct cagccaattg gtatgcgcgc gatgtgaaag ttcgcgaggg catgatggca
     1741 tcactgtcag gagggctggc aaccatgggg cccggcgttc cttattccat cgcggccaaa
     1801 tttgcacacc cccatcgtac ggtcattgcc atggtgggag atggagcgat gcaaatgaat
     1861 ggcatgaacg agctcatcac gatcggcaag tactggcgca gatggagcaa tccgcacttg
     1921 atcgtaatgg tcctcaataa tcgcgacctg aaccaagtga catgggagca gcgggccatg
     1981 gagggcgatc caaagttcga cgcttcccag gatatccccg actttcccta tgccgcgtac
     2041 gctgagctga tcggtcttcg cggcattcgc gtagaccggc cggagcaaat cggcgatgcc
     2101 tgggacagcg ctttaaatgc cgaccgtccc accgttatcg aagcatacac cgatcccgac
     2161 gtgccgcctc ttcccccgca catcacgttc aagcaggcaa aggcgtatgc ctcagcgatc
     2221 ctgcatgggg acaccgattc gccggaaatc atcaagtcgt ctgtaaaaca gatatttgcc
     2281 tgagggggcg gcatgaacgc aaacaccgtt cgcgggcgaa acggaagctc ttcaattccc
     2341 gtatcaagag tacaggtatc cgcattttcc acatcgaccg caacaccgga atccgacggc
     2401 acgctggagt ggaacgcaac cgtgcttgtg ctcgtggaag tcgaagctgg cggcaagacc
     2461 ggtatcggct tcagttatgc agataccggc acagccacgc tcatccacga caccctggcg
     2521 gcggtcgtca cgggccgtga tgcgcttgcc atccccgact gctggaatgc catgattgcc
     2581 gcgatccgca acctcgggcg gccgggcatc gcctccatgg ccgttgcggc agtggataac
     2641 gcattatggg atctgaaagg ccacgttctc gatctgccgc tcgtttcatt gttgggccag
     2701 gcgcggcccg cagtgccgat ttatggtagc ggcggcttta cttcgtattc agtggcggaa
     2761 ctgcaaaaac agctcggcga ttgggcatcc agcggaatag ctcgtgtcaa gatgaagatc
     2821 gggcgggaac ccggcaagga cgtcgcgcgg gtccgtgcag cgcgggaggc gattggcagc
     2881 ggtgtagagc tttttgttga tgccaacggg ggctactccc ggaagcaagc gcttgccatg
     2941 gcattctcgt tttcagaatt tagcgtgtcc tggtttgaag agcccgtgcc gagcgatgac
     3001 cttgaaggct tgcgcctcat gcgtgaccgg gctccgccgg gaatggatat cagtgccggt
     3061 gaatacggtt atcacgccac gtatttccgc cgcatgcttc aagcccgagc agttgacgtg
     3121 ctacaggctg atgcaacgcg ctgcgctggc gtgagcggct tcatgcaggc tgccgcgctc
     3181 tgccatggat tcaacctgcc gctttccagt cattgtgcgc cttcgatcca tgcccatccg
     3241 tgttgcgccg caacccctgt tcgtcatctc gaatacttct acgatcatgt acggatagaa
     3301 aaaatgctgt tcgacggcgt gcttgaaccc gaagacggat tgttgcgccc ggacttgacg
     3361 cgacccgggt ttggtctcga attcaagcgg gcggacgcgg agcggtatgc ggtatgaatg
     3421 gttcacaacg caacagatag aaaataagga atagatatca tgaggcgacc aaccactcca
     3481 cttctggatg cccaggctct ggctgccgag ctgaaaggcg atatccaggg ggaagtacgc
     3541 ttcgatgatg gcagccgggc gctttatgcc accgatggct ccaactaccg gcaggttccg
     3601 attggcgtgg tcattccgaa atcgattgat gacatcatcg caaccgttgc cgcttgccgc
     3661 agacatggcg cgccggtgct ttcccgaggc ggaggcacca gtcttgccgg ccagtgctgc
     3721 aacgtagcgg tggtgatgga catgtccaaa tatttcaacc gaattcttga gctggatccc
     3781 gagaagaaga tcgcgcgtgt cgaaccgggc tgcgtgctcg acaatctgcg ggatgcggct
     3841 gaacaacatc acctgacctt cgcgcccgat ccatcgaccc atacccacaa tacccttggc
     3901 gggatgatcg gcaataactc ctgcggcgtg cactcggtca tggggggtaa aacggtcgat
     3961 aacgtaatcg agcttgacgt attgctttat gacggcacgc ggatgaaggt cggcaagacc
     4021 agtgatgcgg aattggcgga aatcatcgca gcggggggac ggcgcggcga aatctatggg
     4081 cggctgaaat cgatgcgcga caggctggct gaccagatcc gcgcccgcta tccgaaaatc
     4141 ccgcggcggg tctccggcta caaccttgat gatctgctgc cggaaaacgg gtttgatgtt
     4201 gcccgcgccc tcgttggcac cgaaagtacc tgcgtcgtta ttctcgaagc cacgttgcgc
     4261 ctcgtgtaca gccctcctgg ccgctcgctc ctggtactcg gctatcccga cgtatacagc
     4321 gcgggggacc atgttccgga cattctcaag tttggcccga tcgggctcga aggaatcgat
     4381 gaccgcctga ttgcggatat gaaggcaatc ggattgcatc cggaggatgt gcatttgctt
     4441 ccccccggcg gtggatggct tctggcggaa tttggaggcc gcgacaagtc cgagtcggat
     4501 gcttgcgcca acgcgtgcat gaacgcgttg aagcaaacca aagacccgcc aaccatgaag
     4561 ctctttgaca accgtgaaga ggaacagatg atctggaaag tgcgcgaatc cggattggga
     4621 gcaaccgcgc atgtgccgaa caagaatatt acatgggaag gctgggaaga cgccgcagtg
     4681 ccgccggaaa aagtgggcgc gtacctgcgg gattttcgcg cgcttctgga tcggttccat
     4741 tacggttgcg acttgtatgg gcatttcggc cagggatgca tccatacgag gatcgacttt
     4801 gacctggaaa cacacgacgg tatccagatc tttcatgact ttttaagcca cgcagccgat
     4861 cttgtcgtga gctatggcgg ttctctttcc ggcgagcacg gtgacggtca gtccaaggcg
     4921 gaattcctgc cgaaaatgtt tggtaatgaa ctggttgagg cgtttggcga atacaaatca
     4981 atctgggacc cggactggaa aatgaatccg ggaaagattg tggacgccta cttgcccacc
     5041 gagaacctcc gcctcggcac ggcctatgac ccgcctcaac cggataccta tttcaaattt
     5101 ccaggagaca agggaaattt cgggcgggtc acgttgcggt gtgttggaat cggtaattgc
     5161 cggcagcagc attcccagac catgtgcccg agttacaagg tgacccggga agagaagcat
     5221 tcgacacggg gccgggcgcg gctgttattc gaaaccatgc gcggggaggt catcaaggac
     5281 ttttggcaga gcgacgaaat gagggaatcg ctcgaccttt gcctggcctg taaagggtgc
     5341 aagggagatt gcccggtgaa tgtcgacatg gcgacataca aggcggagtt tctctcgcat
     5401 tactacgagc atcggcgccg tccgcgccag gcatatgcgt ttgggttgat agaccgctgg
     5461 gccaggctcg cagcttatgc cccttcagtc gtcaactggt tcactcagac ccgggggctg
     5521 agcgggttgg caaaaagtat cgcgggaatg cctgaacagc gcgccattcc ccgctttgca
     5581 agcgaaacat ttctccaatg gttcagaaaa cgcccgctcc gtaacagcgg aaagcctgaa
     5641 gtcatcttgt ggcccgatac attcaataac catttttatc cgcagaccgc gaaagcagcc
     5701 gttgcggtgc ttgaagccgc gggttttcag gtacgggtgc cggtgcaaca catctgctgc
     5761 gggcgcccat tctatgattt tggcatgctc gaccgggcga aggagcagct ccggcggata
     5821 ttggcgagta tgcgcgagga tattgataat ggtgtgccga tcgttggcat ggagcccgcg
     5881 tgcgtcgccg tgttccgcga tgagctggtc aatctttttc ctgccgacga ggtcgccaaa
     5941 cgtctgaacc gccaaactta ttttttcagc gagtttcttg tcaggcgcgc tcccggattc
     6001 tcttatccca aac
//
LOCUS       DASSHJ010000096        12223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_534513, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000096 DASSHJ010000000
VERSION     DASSHJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534513"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFI43_03865"
     CDS             <1..526
                     /locus_tag="VFI43_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061296.1"
                     /translation="GCVGRRRCLLGTEAALTYQYTATTADEACAKFYDSMAVFLGIEY
                     LRKFFKLKTQCVVGARALNDTYDPAVANGCYATPDSLDEQGTWRRGPAIGSVGMDPGK
                     IIVAQAVLNRCIVKPDGGHDIGHQQAGPQTGIPGCVGILRHCRPSQALGAKVISCDRS
                     LSFGDSASCNPKDS"
     gene            complement(544..1452)
                     /locus_tag="VFI43_03870"
     CDS             complement(544..1452)
                     /locus_tag="VFI43_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7061297.1"
                     /translation="MENRAHALAAGLFVIILSIAVAAAALWFTGDTATRDKYLIVSES
                     PVTGLNPQATVRYRGVTVGKVENIRLDPENHRIVLIRIAVDKNLPLTRNAYAQLGYQG
                     LTGLAFVQLNDESDSAERLSTDNDNLAQIPLRPSALDSITNEGQKLLGKANGLVDRMN
                     VLLGDENQTNFSHILENTAGVTGRLKGVVSQLEPGLKSLPGLAADARSLLTHTDQLVV
                     NLNDITARINEQGGPIDSMSQAAGELTDTLHKFRDVTEGLARNSRSVDRVLLQLEEQP
                     RSLLFGRAAPPPGPGEEGFVPPNGKP"
     gene            complement(1470..2249)
                     /locus_tag="VFI43_03875"
     CDS             complement(1470..2249)
                     /locus_tag="VFI43_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7061298.1"
                     /translation="MSNAECIVKVEGLHTRFGTHVVHEDINLCVRRGEVLALVGGSGE
                     GKTALMREMLGLDAPTQGTIQVFGEPLQGQDRKKQQQMRNRCGVLFQKGALFSALSVY
                     DNIALPLRELHTLDEAMIRDLVMLKLKMVEIAPAHATKMPSELSGGMIKRIALARAMA
                     LSPELLFLDEPTAGLDPELSEGFVKLIQAMRRELTLTIVMATHDLDTLIALADRIAVL
                     AQRRMVALGTLEEVIRTEHPFVQNYFCGERGKRTIAGQRNS"
     gene            complement(2265..3404)
                     /locus_tag="VFI43_03880"
     CDS             complement(2265..3404)
                     /locus_tag="VFI43_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7061299.1"
                     /translation="MEPENQAGTSSITWRRITGADGSPYLMLSGSLTLAALGNRLELV
                     LAALSKFRGAQELHWDLTGLTQLDDAGAVLLWRAWGEHRPTYLILRPDHDRFFARAEE
                     MRPPTAPVRADWLWPINKVGAAVFLLWEHFVGLLTLSGQLSLDAIYLLSHPARIPWRE
                     ISANLYRTGAKALGITALVGFLIGVVLSYLSSRQLQTYGADVFIINILGISIIRELGP
                     MLAAILVAGRSGSSMTAQLGVMRVTEELDALTVMGIPHSLRLVLPKVLALGIAMPLVV
                     LWTSAVALLGGMVIAEVQLGLSYYFFLSKLPDSVPIANLWLGLGKGVVCGMVIALISC
                     HFGLRIKSNTESLGEGTTNSVVTAITVVIIIDAIFAVVFSNVGLR"
     gene            complement(4036..4515)
                     /locus_tag="VFI43_03885"
     CDS             complement(4036..4515)
                     /locus_tag="VFI43_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061300.1"
                     /translation="MEKFMALVLSMLAFTATADEIDKRQVLSLTEHQRDHVLTEMRAM
                     LSGTQKILDALSREDMQAVATEARSLGMGMTDKAEGHLKSVLPKDLMQLGMSVHKAFD
                     KIAEDAESLKDPKHTLRQLVDSMQKCTACHASYQIRVKTHAKGQSPAPDGQHLHHQH"
     gene            complement(4657..5994)
                     /gene="rimO"
                     /locus_tag="VFI43_03890"
     CDS             complement(4657..5994)
                     /gene="rimO"
                     /locus_tag="VFI43_03890"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7061301.1"
                     /translation="MALPRQQPPRIGFVSLGCPKALVDSEQILTQLRAEGYETSSSYE
                     EADLVVVNTCGFIDRAVEESLDAIGEALAENGKVIVTGCLGAKDGGDVIRQAHPQVLA
                     VTGPQALPEVMAAVHEHLPQPHDPFTSLVPPQGIRLTPRHYAYVKISEGCNHRCTFCI
                     IPSMRGNLVSRPVHQIMQEAENLVDAGVKELLVISQDTSAYGVDINYRIGFWKGRPLK
                     ARLAELASALGGLGVWVRLHYVYPYPHVDEIIPLMADGKILPYLDVPFQHASPRVLKA
                     MRRPANTENNLMRIRKWRDICPDITLRSTFIVGFPGETEAEFEELLGFLEEAQLDRVG
                     CFAYSPVEGAAANALPDPVCEEVKEERRARFMALQEKISQARLSQKIGKRMTVLVDEV
                     RKNGAIARSTADAPEIDGIVHIARSKNLKAGEFAQVEVTGSDTHDLRAKAVTA"
     gene            complement(6078..6884)
                     /locus_tag="VFI43_03895"
     CDS             complement(6078..6884)
                     /locus_tag="VFI43_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET7061302.1"
                     /translation="MSVLSWIIATTLFGALLSVLVAAVLTLNTRASWVQMLISYAIGA
                     LLGAAFLNALPEALELSKNPAQMTGTVLFGILLFFILEKLVLWRHCHSAECDAHDRPI
                     APVTARPATSHGHDNGRSGMMIMLGDTFHSFVDGVLIAAAFMADIQLGIVTSIAIIAH
                     EIPQEAGDFLILLNSGYTRRQALLFNMFSSAATLLGGLATYFMLQDMNYIIPSLLGLA
                     SASMIYVAMSDLIPGLHKRPEIDATIQQVALITLGISSIWLAGSLFTHHA"
     gene            complement(7023..7781)
                     /gene="pgeF"
                     /locus_tag="VFI43_03900"
     CDS             complement(7023..7781)
                     /gene="pgeF"
                     /locus_tag="VFI43_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET7061303.1"
                     /translation="MTIPSLEAGSWIVPDWPAPPNVKALFTTRKGGASTGPYASFNLG
                     DHVGDDPLAVAKNRGILRQVLPEEPRWLRQVHGVHPVDVDDPHCTSSGADAAFSRHQG
                     NVCVVLVADCLPILVCDRAGTVTAAIHAGWKGLVNGVVEQTLSNVGVESEYLMAWLGP
                     AIGPRHFEVGEEVRQAFIEHDMNAAHAFVQHRHKPGKWFADLFLLARQRLTTAGVHEI
                     HGGGKCTFSDPERFFSYRRDGTTGRMAGLVWLTA"
     gene            complement(7778..8740)
                     /gene="rluD"
                     /locus_tag="VFI43_03905"
     CDS             complement(7778..8740)
                     /gene="rluD"
                     /locus_tag="VFI43_03905"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HET7061304.1"
                     /translation="MPETVQRPFELTIPPDYAGLRLDQALARLLPDQSRSRLQAWILS
                     KQVTVENWEATAKHRVWGGEKVHINPVPFPAESAYAPEAISLDILHEDESLVVVNKPA
                     GLVVHPGSGNWQGTMLNALLHHVPQLGGIPRAGIVHRLDKDTSGLLVVAKTLEAQTSL
                     VRQLQERTVKRIYIALVLGDVTDQGWVDAPIGRHPVHRTKMAVVVGGKEARTHFQAIE
                     KLEGCTLLRCSLETGRTHQIRVHMHSLGHPLVGDPVYGGKPRQLAPQAGQLISDFPRQ
                     ALHAKQLELAHPRTGSKMTWDAPLPEDMSSLLLMLRQPGSGNTL"
     gene            8796..9608
                     /locus_tag="VFI43_03910"
     CDS             8796..9608
                     /locus_tag="VFI43_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET7061305.1"
                     /translation="MAHGAGLAILLIVPIMLSGCGLLPKKPEDTKNWSASKYYSEAKN
                     ELNEGNYSAAVKLFEQLEARYPYGRYAQQAQLEIAYAYYKDSEQAQAIAAADRFIKLH
                     PNHINVDYAYYLKGLANFNDDIGLMGIVSEKILNQDMSERDPKASRESFENFRELVAR
                     FPKSKYAPDAIQRMKHLVNVVALNEVQVARYYMKRGGYIAAANRAQYALKEYPQTPAI
                     EEALFIMVQAYDKLGMTDLRDDAARVMKKNFPDSRFFSGGVAKGSESWWKFW"
     gene            complement(9685..10179)
                     /locus_tag="VFI43_03915"
     CDS             complement(9685..10179)
                     /locus_tag="VFI43_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7061306.1"
                     /translation="MKVDYRHVLVAVDLSEQDTYVVGKAKALAQVFQAKLSVVHVLDN
                     IPMPDTAYGTVIPLDQNSSYEMLEAVKTRFQRLADSLAIDPERRWMVWGVPGEEIVRV
                     ARQEGVDLIMVGSHGRHGLGLLLGSTANGVLHHAECDVMAVRLREEVERRESLQEFAP
                     NPSC"
     gene            complement(10241..10915)
                     /locus_tag="VFI43_03920"
     CDS             complement(10241..10915)
                     /locus_tag="VFI43_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HET7061307.1"
                     /translation="MKNFLLGLFILTTAAATDSGAATAPASKTGTTTIMVYGDSLSAG
                     YGLPQEAGWVNLLKKRVQTVSQVAVINNSISGETTTGGRNRIEQALKTHRPDIVIVEL
                     GGNDGLRGASIDSIRDNLQAIIEACQRNKATVLLTGMQLPPNYGMLYTQKFQDMYHQL
                     AKRYELKLVPFLMAGFGDKRHFFQADGIHPTAQAQEIIVENVWKVLRTMLKSGQAVAY
                     EGNSRR"
     gene            10914..11600
                     /locus_tag="VFI43_03925"
     CDS             10914..11600
                     /locus_tag="VFI43_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061308.1"
                     /translation="MSNNLVVRPIVQATGLTKEVSTGDEKLTILENINLEVNGGDSVA
                     IIGASGSGKSTLLGLLAGLDTPSSGKVHLDGQDIFSLDEDARAAVRGRMLGFVFQSFQ
                     LLPALTAIENVMLPLELAGAVDIHTPALELLERVGLGMRLKHYPRQLSGGEQQRVAIA
                     RAFVTQPQLLLADEPTGNLDSATGAQIIELMFELNRERGTTLILVTHDEAISSRCHWR
                     IRLSGGKIVN"
     gene            11678..>12223
                     /locus_tag="VFI43_03930"
     CDS             11678..>12223
                     /locus_tag="VFI43_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET7061309.1"
                     /translation="MEAISQRKPDERRKPRLKAVWFAILVASVIASTWVWRNQSNATA
                     VKSKPVEAIPVLSAAVKQADVPVTLTANGTVSPQQTVAVRPQLSAVIRSVHIKEGQFV
                     KKGERLFTLDARTEDANLRRTEGQLAKARADLRNAQRNLERQRELFRQNFISQAALDL
                     AENLVEAMGGQLAVDEATVQAN"
BASE COUNT         2754 a         3372 c         3311 g         2786 t
ORIGIN      
        1 cgggtgcgtt ggccgacggc gctgcttgct cggcactgaa gctgcgctga cttatcaata
       61 cacggctacc acggctgatg aggcttgcgc gaaattttac gacagcatgg ctgttttcct
      121 cggtatcgaa tacctgcgta aattcttcaa gctcaagacg caatgtgtag tcggtgcgcg
      181 cgccctcaat gacacttatg accccgccgt cgctaatggc tgctatgcga ctcctgattc
      241 gctggacgag cagggaacct ggcgtagagg cccagcgatt ggcagcgtag gaatggaccc
      301 gggcaagatc attgtagccc aagcggtatt gaatcgctgt attgtcaagc cagacggggg
      361 tcatgatatc gggcaccagc aggctggccc gcagacgggg atacccggat gcgtcggaat
      421 tctccgacac tgccggccca gccaggcgtt gggcgccaaa gtcataagtt gcgaccgcag
      481 tttgagcttt ggggatagcg catcctgcaa tcccaaggat agctaagatc aatagctttt
      541 tcatcaaggt tttccgttgg gagggacaaa accttcctca cccgggcctg gaggaggggc
      601 agccctaccg aatagcaagc tccgaggttg ctcttccagt tgcaggagga cacgatccac
      661 gctgcgcgaa ttacgcgcaa gcccctccgt tacatcacga aacttgtgca gcgtgtcagt
      721 aagctcgccg gctgcttgag acatgctgtc gatcggtcca ccctgctcgt ttatccgggc
      781 agtgatgtcg ttgagattca cgacgagctg atcagtgtgc gtgagcaggg accttgcatc
      841 agcggccaga ccgggcagag atttcagacc gggctcaagt tggctcacca ctcctttcag
      901 gcgcccggtt acgcccgcgg tattttcaag tatgtgcgaa aaattcgtct ggttttcatc
      961 tcctagcagc acgttcatcc ggtccaccaa tccgttggcc ttcccaagca gcttctgtcc
     1021 ctcattcgtt atactgtcca gcgcggacgg ccgcagggga atttgagcca ggttgtcatt
     1081 atccgtgctc agtcgttcag cactgtcact ctcatcgttc agttgcacaa atgcaagccc
     1141 ggtcagacct tgatagccaa gctgagcata tgcattcctg gtcagcggca ggttcttgtc
     1201 gacagcaatg cgtatcagga cgattctatg attttcggga tcgagccgaa tattctcgac
     1261 ttttccgacg gtgactcccc tgtaacgcac ggtcgcctgg gggttgagtc ccgtcacggg
     1321 agactccgat acgatcaggt atttatcccg ggtggcagta tccccggtaa accacaaggc
     1381 ggcagcggcc acggcgatac ttagaattat cacaaataga cctgcagcaa gggcgtgggc
     1441 acggttttcc atatcttccc ttaggttcct taagaattcc tttggccggc gattgtccgt
     1501 tttccgcgct cgccgcaaaa atagttctga acaaaggggt gttccgtcct gatcacttcc
     1561 tccaacgttc ccaaggccac cattcgccgc tgcgccagga cggctatccg gtcggcaagc
     1621 gcaatcaggg tatcaagatc gtgcgttgcc ataacaatgg tcagggtcag ttctcgtcgc
     1681 attgcctgga tcagttttac aaacccctcg ctcagttcgg gatcaagccc ggcggtaggt
     1741 tcgtccagga acagcagctc gggactgagc gccatcgcac gtgccagtgc aatacgcttt
     1801 atcatcccac ccgagagctc ggagggcatt ttggttgcgt gcgcaggtgc aatttcgacc
     1861 attttaagtt tgagcatgac caagtcacga atcatcgcct cgtccagggt gtgcagctca
     1921 cgcagaggca aggcgatgtt atcgtatact gacagtgcac tgaacaaggc gcctttctga
     1981 aacagcactc cgcaacggtt gcgcatctgc tgctgctttt tccggtcctg gccttgcaat
     2041 ggttccccga aaacctggat cgtaccttgc gtaggcgcat ccaggcccag catctcacgc
     2101 atgagcgcgg ttttaccctc gcccgaccct cccacaagcg cgaggacctc gccacggcgc
     2161 acgcaaagat tgatatcttc gtgaaccacg tgcgtgccaa agcgggtatg cagtccctct
     2221 accttgacaa tacattccgc gttcgacatt agatatcggg atcgctacct tagcccaaca
     2281 ttggaaaaaa ctactgcaaa aattgcatcg atgataatga cgacagtgat cgccgtgact
     2341 accgaatttg tcgtgccttc gcccaggctc tccgtattgg atttgatcct tagaccgaaa
     2401 tggcaggaaa tcaatgcgat caccatccca catacgactc ccttgcctag tccaagccaa
     2461 agattggcaa taggaaccga atcgggcaat ttacttagaa aaaagtaata actcagtccc
     2521 agctgaacct ctgcgatgac cattcccccc aataacgcga cagcgcttgt ccataatacg
     2581 accaggggca tcgcgatacc cagcgcgagc accttgggga gcactaacct caggctgtgc
     2641 ggaattccca ttaccgtcag cgcgtcgagc tcttccgtta cccgcattac tccaagctgg
     2701 gcggtcatgg aagaccctga gcgccctgcc accagaatcg cagcgagcat tggcccaagt
     2761 tcgcggatga tgcttatgcc gagaatattg atgataaaga catccgcgcc ataggtttgc
     2821 agttgccgcg acgaaagata gctcagcacg accccgatga ggaagccaac cagcgcggtg
     2881 atccccaacg ccttcgcgcc tgtccggtac aggttcgccg aaatctctcg ccagggaatc
     2941 cgggcaggat ggctcagcag atatattgcg tcgagcgata attggcccga cagcgtcaat
     3001 agcccaacaa agtgctccca aagcagaaag acggccgccc ccaccttgtt aatcggccat
     3061 agccaatcgg ctcggacggg cgccgtggga ggccgcattt cttcggcgcg ggcaaaaaac
     3121 cgatcatggt cgggccggag tatcaggtat gtgggcctat gttcccccca tgcgcgccat
     3181 aacaggactg cgcccgcatc gtcgagttgg gtcaaccccg tcaggtccca gtgtaattcc
     3241 tgcgctcccc gaaattttga aagagctgcg agcacgagtt ccagccgatt tccgagcgcg
     3301 gcgagcgtaa ggctcccgga aagcatgaga tatggggagc catctgcgcc ggttatacgc
     3361 cgccaggtga tggacgaggt gccagcttgg ttctcaggct ccattattgt tcctaatacc
     3421 cgatttacgg caatcgtgga ggcgcttggc tgtgcccggg ctgtatgctg gacgcgaagc
     3481 tcgtcatttc ctgctagtag aactcatact ctgttttcac gtcaagcctt gaccctaatc
     3541 atcgccattg cttctgcaaa gacaacttta taccgcggcg catagagaga gcgctaacgg
     3601 gtgaaaaaag tgacggcaaa ccttacattt gctctctttt tccgagcagt taaacgataa
     3661 cccgctgttt aaacgggtat gtaaaaactg gcacgcactt tgcttaaaga aaagcgcgac
     3721 aagtacttgt cggggttcgg ggagcagaag aaaaagaaat tgctgcggta aggcaatgac
     3781 gaattcggaa tggaatggat ggtcccgctc cgagccatat aacaataaaa aagctgttta
     3841 taacaataaa aatcgcacaa tcagggttgg gagaatcttt ccgaaaggaa attgttctga
     3901 ccctgattat tttttcaatg cgattaatgc gtttctgatt tcctcaacag attcgccaag
     3961 tccttaacag ttatagccgc gccttgcaac tgaagttttt ccaaacaatt tttccgaaca
     4021 attgaaggcg tctctctagt gctgatggtg caaatgctgc ccatcgggag ctggcgactg
     4081 accttttgca tgagttttta cccgaatttg atagctcgcg tgacaggcag tgcacttttg
     4141 cattgagtca accagttggc gcagggtatg tttggggtct ttcagggatt ccgcatcctc
     4201 cgcgatcttg tcgaaggcct tatggacgga catgccgagt tgcatcaaat ccttggggag
     4261 aactgacttg aggtgacctt ccgctttatc cgtcatcccc atgccaagcg aacgggcttc
     4321 ggtcgccacg gcctgcatgt cttccctcga cagtgcatcc aggatctttt gagttcctga
     4381 cagcattgcc cgcatctccg tgagtacgtg gtcgcgctgg tgttcggtta gcgacagtac
     4441 ctggcgtttg tcgatttcgt ccgcggttgc ggtaaacgcg agcatgctca ataccagcgc
     4501 catgaatttt tccaacttga cctccagcgt ttaatggttg acgttcccgt tttttccggt
     4561 tttattgccc cttactacgt ttcttgcaga cacctcatcc atgtcatcta tgcttcacac
     4621 cgttttgctg aaaaagcaac tcggcggggg acggtatcaa gcggttacag cttttgcccg
     4681 caaatcgtgt gtatccgagc ccgttacttc cacttgtgcg aactcgccgg ctttcaggtt
     4741 ttttgaacgt gcgatgtgaa cgatgccgtc tatttcaggc gcatccgccg tgctgcgcgc
     4801 aatagcgcca ttctttcgga cttcgtccac aagcaccgtc atgcgcttgc cgattttttg
     4861 actcaagcgg gcctggctga ttttttcctg caaagccatg aaccgcgcac gccgttcttc
     4921 ctttacctct tcacataccg gatcaggcaa ggcattggca gcagcgcctt ccactggtga
     4981 ataggcgaag cagcccacgc gatccagttg tgcctcttcc agaaaaccca gcaactcctc
     5041 gaactctgct tcggtttcac cgggaaatcc aacaatgaac gtgcttcgga gcgtgatgtc
     5101 cggacatatg tcgcgccact tcctgatccg catcagattg ttttccgtgt ttgccggacg
     5161 tcgcattgct tttagaacgc gcgggcttgc atgctgaaac ggtacgtcga gataaggaag
     5221 aatctttcca tcagccataa gtgggattat ttcgtcgacg tgcgggtagg gatacacgta
     5281 atgcaagcgc acccatacgc ccaatccgcc tagcgcgctg gccaattccg caagccgggc
     5341 cttgagcggc ctccctttcc agaagccgat gcgatagttg atatccactc cataggcgct
     5401 ggtatcctgg gaaatgacca gcagttcctt tactcctgca tccaccagat tttcagcctc
     5461 ctgcatgatc tgatgaaccg ggcggctcac gagattgccc cgcatgcttg ggatgataca
     5521 gaaagtgcag cgatggttgc agccttccga gatcttgaca tacgcgtagt gccggggtgt
     5581 caggcgtatt ccctgcgggg gaacaagact ggtgaaaggg tcatgcggct gaggcaggtg
     5641 ctcatgaaca gcagccatca cctcagggag cgcctgcggg cccgttaccg ccaatacctg
     5701 aggatgcgcc tgcctgatta cgtctccacc gtccttcgcg ccaaggcaac ccgtaacgat
     5761 gacttttcca ttttcggcca acgcttcgcc aatcgcatcc agcgattcct ccaccgcccg
     5821 atcgatgaat ccgcaggtat tgacgactac gagatcggcc tcctcgtacg aagaagaggt
     5881 ctcatatcct tcggcacgta actgagtgag gatttgctct gaatcgacca gtgcctttgg
     5941 gcaaccgagc gatacgaaac cgattctggg gggctgttgt cggggcagtg ccataacgta
     6001 atcaggagca aataaaagag tgaggattat ccatgagctg gggctttgaa atcaaaacgc
     6061 caggaacgtt caacggttta tgcgtgatgc gtaaagaggc tgccggcaag ccagatcgag
     6121 ctgattccaa gcgtgatgag cgcgacttgc tggatggtgg catcgatttc cggtcgcttg
     6181 tgcaggcctg gaatcaaatc cgacattgcg acatagatca tgctggctga agccagcccc
     6241 aggagggagg ggatgatgta gttcatatcc tgcaacatga aatacgtggc cagtccgcca
     6301 agcagtgtag cggcgctgga gaacatatta aagagaagcg cttggcggcg ggtatagcct
     6361 gaattgagaa gaatgaggaa gtcccctgct tcctgtggaa tttcatgggc gatgatggca
     6421 atcgaagtga caatcccaag ctggatatct gccatgaacg ccgccgcgat cagtacgcca
     6481 tccacgaaac tgtgaaaggt gtcccccagc atgatcatca tgccacttct tccattatca
     6541 tgaccgtggc ttgttgccgg acgggcagta acaggcgcaa tcggccggtc atgggcgtcg
     6601 cattccgccg aatggcagtg ccgccacagc acgagcttct ccagaatgaa gaacagaaga
     6661 atgccgaaca gtacggtacc ggtcatttgt gcgggatttt tcgacaactc gagcgcttcg
     6721 ggcagcgcat tcagaaaggc cgccccgagc agcgcgccga tagcgtaaga aatcagcatt
     6781 tgcacccacg aggcacgcgt attgagcgtg agcacggccg ctaccaatac gctcagtaaa
     6841 gcgccaaaca gggtcgtggc aatgatccag gagagaacgg acatcggggc aggggaaaat
     6901 ccgcgattat acgcggttgc gagaacttgc tttagttcat tgaagccagc ttgtgtaggg
     6961 aagcgagttc tttgcgtggc atcggcgatg tcgcagcgcg atcctgctca atgctgtccc
     7021 ttttaggcag tgagccaaac gagtcccgcc attcgtccag ttgttccgtc gcggcggtag
     7081 gaaaaaaatc tttcgggatc gctgaatgtg catttccctc cgccatgtat ttcatgaaca
     7141 cccgctgttg tcagccgttg acgtgccagc agaaaaaggt ctgcaaacca tttgcctggt
     7201 ttgtgccgat gctgaacaaa tgcgtgggcg gcgttcatgt catgctcgat gaatgcctgg
     7261 cgtacctctt cccccacttc aaaatgcctg ggcccgattg cgggaccgag ccaggccatg
     7321 agatattcgc tttccacgcc cacgttcgat aatgtctgtt cgaccacgcc gtttacgagg
     7381 cctttccagc ctgcatgaat ggcagccgtt acggttcccg cacggtcgca cacgagtatc
     7441 ggcaggcagt ctgcgacaag tacgacgcag acgttgccct ggtggcggct gaaggccgca
     7501 tcggcccctg aagatgtgca gtggggatca tccacatcaa caggatggac gccatgcacc
     7561 tgtctcagcc accttggctc ttccggaaga acctggcgca gtatgccgcg gtttttcgca
     7621 acggccaacg gatcgtcgcc gacatggtca ccgagattga agctggcata aggtcccgtg
     7681 ctggctcctc ctttgcgggt tgtgaacagt gctttgacgt ttggaggcgc gggccagtca
     7741 ggcactatcc agctgccggc ttctaacgaa gggattgtca tagtgtattc ccactaccgg
     7801 gttggcgcag catcagcagc aagctgctca tatcctcagg cagaggcgca tcccatgtca
     7861 ttttgctgcc ggtgcgagga tgggccagtt caagttgctt cgcatgcaaa gcctggcgag
     7921 ggaagtccga aatgagctgc cccgcttggg gtgcaagctg cctgggcttg cctccataaa
     7981 caggatcgcc cacgagcgga tgtccaagcg agtgcatatg gactcgtatc tggtgagtgc
     8041 gaccggtttc aaggctgcac ctgagcaagg tacagccttc gagcttttcg atggcctgaa
     8101 aatgggtgcg ggcttccttg cctcccacga cgacagccat ttttgtacga tggacaggat
     8161 gcctgcctat tggcgcatcg acccatccct gatcggttac atcccccagc acaagcgcaa
     8221 tgtagatacg tttcacggtg cgctcctgca gttgccgaac caggctggtt tgcgcttcca
     8281 gggtttttgc gaccaccagc aacccgctgg tatccttgtc gagccggtga acgatgcccg
     8341 ctcttggtat tccccctaat tggggtacgt gatgcagcaa ggcgttcaac atggtcccct
     8401 gccagttccc gctgccggga tgtaccacca acccggccgg cttattgaca acgacgagcg
     8461 actcatcctc atgaagaatg tcaagagaga ttgcctcagg cgcgtaagcg ctttccgccg
     8521 ggaaaggaac ggggttgatg tgaaccttct ctcctcccca cactctatgt ttcgcggttg
     8581 cttcccaatt ttcgaccgtt acctgcttgc tgaggatcca cgcttgcaat ctgctccggg
     8641 actgatcggg gagcaatctg gccagcgcct gatccagccg tagacccgcg taatcagggg
     8701 ggatggtcag ctcgaagggg cgttgaaccg tttcaggcat cgaaaggttt ggcgctataa
     8761 tgaaactctc ccattactct tcttcagaaa ttcatatggc acatggtgca ggattagcaa
     8821 ttcttcttat tgtgccgatc atgttatcgg gttgcgggct gttgcccaag aaaccggaag
     8881 atacgaaaaa ctggtccgcg agcaagtatt attcagaggc aaagaacgaa ctgaacgaag
     8941 gaaattactc ggcggccgtt aaactattcg aacagctcga ggcccgctat ccttacggac
     9001 gctacgcgca acaggcgcag cttgaaattg cgtacgccta ttacaaggac agcgaacagg
     9061 cccaggcgat tgccgctgct gatcgcttca tcaagcttca tccgaatcat atcaacgtcg
     9121 attatgccta ttatctcaaa ggcctcgcca attttaatga cgatatcggt ttgatgggca
     9181 ttgtctcgga aaaaatcctg aaccaggata tgagtgagcg ggatcctaag gcttcacgcg
     9241 aatcatttga aaattttcgc gaactggtgg cccgttttcc caagagtaaa tatgccccgg
     9301 atgcgatcca gcgaatgaag cacctggtaa acgtagtggc gttgaacgaa gtccaggtgg
     9361 cccgctacta catgaagcgg ggaggttata ttgcggcagc caaccgcgct caatacgcgc
     9421 tcaaggaata tccccagact ccagcgatag aagaagcgct gtttatcatg gtgcaagcgt
     9481 atgacaagct gggtatgaca gatctgcgcg atgacgcggc tcgcgtgatg aaaaagaact
     9541 ttcccgacag ccggttcttc tccggcggag ttgcaaaggg cagcgagtcc tggtggaaat
     9601 tctggtaagc gacatgagaa ttcgccaaag atcgcgtctc aacctctcag cctctcaatg
     9661 agcagcgact tgcggtcgct acgctcagca tgaaggattc ggcgcgaatt cttgcagtga
     9721 ctcacgacgt tcgacttctt cccgcaaacg aactgccatg acatcgcact cggcgtgatg
     9781 cagcacaccg ttcgccgtag acccgaggag cagacccaga ccgtgccggc catgggaccc
     9841 aaccatgatg agatcgacac cttcctggcg ggcaacccga acgatttctt cgcctggaac
     9901 gccccatacc atccagcggc gttccgggtc gatcgcaagc gagtccgcga gccgttgaaa
     9961 tcttgtcttg actgcttcaa gcatttcata cgaagaattc tgatcgagag gaataacggt
    10021 gccgtaagcg gtatcaggca tcgggatgtt atcgagaaca tgaacaacgc tcaatttcgc
    10081 ctggaaaacc tgggcaagag ccttggcttt tcccacaaca taagtatcct gctccgacag
    10141 gtccacagcc accaggacat gcctgtagtc aactttcatt gattatccat tcgatatgcg
    10201 atcggatccg caaacccgtc tcggcattct ttaaaccgcg ttatcgacgt gagttgcctt
    10261 catacgcgac cgcctgcccc gatttaagca ttgttctcag gaccttccag acgttttcaa
    10321 caattatttc ttgcgcctgc gcagtcgggt gtatgccgtc cgcctgaaaa aaatggcgct
    10381 tgtcaccaaa tccggccatg aggaacggga cgagcttgag ctcgtagcgt tttgcgagtt
    10441 gatgatacat gtcctgaaac ttttgcgtgt ataacatgcc atagttgggc gggagctgca
    10501 tgccggttaa aagaacagtc gccttgtttc gctggcaagc ttctatgatt gcttggagat
    10561 tgtcccgtat ggagtcgatg gatgcaccgc gaagcccgtc attaccccca agctctacaa
    10621 tgacaatatc tgggcgatga gtcttcagcg cttgttcaat gcggtttcgc ccgccggtcg
    10681 tcgtttcacc gctaatgctg ttgttgatca cagcaacctg tgataccgtt tgcacccgct
    10741 ttttcagaag gttaacccac ccagcctcct gcggaagtcc atagccagcg gacagactat
    10801 ccccgtacac cataatggtt gtggtgccgg tctttgaagc cggcgccgtt gcagcgcccg
    10861 agtcggtggc agcagcagtt gttaaaataa atagtccgag caggaaattt ttcatgtcaa
    10921 ataaccttgt tgtacggcct atcgtgcagg caaccggcct gacaaaggaa gtcagcaccg
    10981 gtgacgaaaa gcttacaatc ttggagaaca tcaatctgga agtcaacggc ggcgattcag
    11041 tcgccatcat tggcgcgtcg ggctcgggca agtcgacctt gctcggcctg ctcgcggggt
    11101 tagatactcc ttcgagcggt aaggttcacc tggatggtca agatatcttt tccctggatg
    11161 aggatgcccg cgctgcggta cgcgggagaa tgctgggttt tgtgttccag tcattccaat
    11221 tgctgcctgc cctgaccgcg attgaaaacg taatgctgcc cctagagctg gctggggccg
    11281 ttgacatcca tacccctgca ctggaattgc tggaacgggt agggcttgga atgcgtctca
    11341 agcattatcc caggcagttg tcggggggtg agcagcagcg cgtcgccatt gcgcgggctt
    11401 ttgtaactca gccacagttg ctgcttgccg acgagccgac cggtaacctg gactccgcca
    11461 cgggagcgca aatcatcgaa ctgatgttcg agctaaaccg tgaacgaggc acaaccctga
    11521 ttctcgtcac gcatgacgaa gcgatttcca gccggtgcca ttggcggata aggttgtccg
    11581 gggggaaaat tgtaaattga cgcgccattg ctgcagccga tccactcggt ctgctccggt
    11641 tgtcacagcc gcaagaatga aattctacgc aggttgaatg gaagccattt ctcaaaggaa
    11701 accggatgag cgccgaaagc cgcgtctgaa agcggtatgg ttcgcgattc tggttgcaag
    11761 cgtgattgcg agcacctggg tatggcgcaa ccaaagtaat gctaccgctg taaagagcaa
    11821 gcccgtcgag gctattcccg tgttgagcgc ggcggtaaag caggccgatg tcccggttac
    11881 gttaacagcc aacggaaccg tgtccccaca gcagaccgtt gcagtacggc cacagctcag
    11941 tgccgttatc cgctctgtgc acatcaagga agggcagttt gtgaaaaagg gcgaaaggct
    12001 gtttacgctg gatgcgcgta cggaagatgc gaacctgcgc aggaccgagg ggcaactcgc
    12061 gaaggcgcgc gcggatctgc gcaacgcaca gcgcaatctc gaacgccagc gcgagctttt
    12121 tcgtcagaat tttatttcgc aggctgcgct tgatctcgcc gagaatctgg ttgaggcgat
    12181 gggcggacaa ctggcggtcg atgaggctac cgtgcaggcc aat
//
LOCUS       DASSHJ010000097         4501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1330346,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000097 DASSHJ010000000
VERSION     DASSHJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330346"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..929)
                     /locus_tag="VFI43_03935"
     CDS             complement(75..929)
                     /locus_tag="VFI43_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HET7061310.1"
                     /translation="MNKSSFRLLPLVPLVSALFVIGAPAAQAVGLEQSPIDITSNNTV
                     YNPNLPKLNFNLSSDAGLKVINNGSPAVDKTAVRANLDDPSASTLSVNGDTYTLSQFH
                     FHAPAEHLENGYKYPMEMHLVFQDANKHLLVVGRWVQEGSFNQTLDPIFSHIPQDTST
                     TLPVSHFNLNALLPDHLNSFRYNGSLTTPDFGEGVKWVDLSTPMDMSKEQINAFSSLF
                     PNGDAREVQALNGRIVQTDLPGFTVTSIPEPETYAMMLVGLGMIGFIANRRRRMNSLQ
                     SGVLPAVV"
     gene            complement(1150..2568)
                     /gene="zwf"
                     /locus_tag="VFI43_03940"
     CDS             complement(1150..2568)
                     /gene="zwf"
                     /locus_tag="VFI43_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7061311.1"
                     /translation="MAETDDSEQKRARSDALVLFGVTGDLAYKKIFPALYAMIKKGLL
                     NVPVVGVASTPWNIDQLRERATDAVKHANAEKIDDPAALSRLLSLLKYIDGDYNDPGT
                     FKKLKQALGNVKRPVHYLAIPPSLFETVIKNLGAAGLAHGARVIVEKPFGRDLASARE
                     LNRIALSVFPEDAIFRIDHFLGKEAIMNILYFRFANSFLEPIWNRNYVASVQLTLAEN
                     FGVEERGAFYESAGCLRDVIQNHLFQIVALLAMEPPPFEGFGAVQGEKASVFQAMRPL
                     KPDDLVRGQYQGYRSEPGVAKNSDVETFCALRLFIDSWRWEGVPWYLRSGKFLAETAA
                     EVLVQLKPPPQKLFADSASQAEYANYLRFRLSPISAIALAARVKRTGKEFVGDQRELC
                     LVEEHFGRESPYERLLRDAMNGEGALFAREDAVEAAWIVVEPVLKNHHPVIPYERGSW
                     GPKAADMLIAPDGWWHNPESRI"
     gene            complement(2570..3589)
                     /gene="gnd"
                     /locus_tag="VFI43_03945"
     CDS             complement(2570..3589)
                     /gene="gnd"
                     /locus_tag="VFI43_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET7061312.1"
                     /translation="MQIGMIGLGRMGVNMTARLMKAGNECVVFDTRPEAVQELQSAGA
                     EGATSLKDFISRLSKPRAIWLMVPAAVVDKLISELLPLVSPGDIIVDGGNSYYRDDIR
                     RAADLNAKGVHYVDVGTSGGVAGRERGYCLMIGGEKEIVQHLDPIFAALAPGIDSAPR
                     TSTLTRSDGTAEQGYLHCGPHGAGHFVKMVHNGIEYGLMAAYAEGLNILHNANAGDRK
                     RDTDAETAPLRDPEYYQYAFDIPQIAEVWRRGSIVASWLLDLTAEALAEDAKLSAFSG
                     HVSDSGEGRWTTLAAIDEGVPVPVIAAALFSRFESRGNAVFAAQVLSAMRKRFGGHDE
                     KKGGA"
     gene            complement(3732..>4501)
                     /locus_tag="VFI43_03950"
     CDS             complement(3732..>4501)
                     /locus_tag="VFI43_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7061313.1"
                     /translation="LLLGVNRSRRPADRQHPFGYGKELYFWSLIVAIMLFGIGGGMAI
                     YEGVDHVINPRPLEDPFWAYVVLVVSMLLEGISWTFAMRALLKEKGKYTLWQTLRRSY
                     DPAVLTVLLEDTAALIGLAVAFFGIYLAHRFSNPYYDGGASVLIGVTLCVVSVILIRE
                     SKGLLVGEAATPEVVESVISLARNEPAVVAVNQVLSMVFGPEDVLLNIEAQFVPSLSV
                     SELAKAVSRLEVAIQQQHPRVKRIFIEVAPAGPHHAS"
BASE COUNT         1028 a         1262 c         1215 g          996 t
ORIGIN      
        1 gttatgcctg attcccccac ctttcctgat ggtgggggaa tcaggcgcgg aagcgcttga
       61 tgcgacagga ggcactatac cactgcaggc agaactccgg attggagcga gttcatccgt
      121 cttctacggt tcgcgatgaa gccgatcatg ccaagaccca ccagcatcat ggcataggtt
      181 tccggctccg gaatcgaggt gacagtaaat ccgggcagat ctgtttgtac gatacgcccg
      241 ttgagcgctt ggacttcgcg agcgtcacca ttcgggaaca atgaactgaa tgcgttgatc
      301 tgttcctttg acatgtccat tggtgtcgac agatcgaccc atttcacgcc ttcgccgaag
      361 tctggcgtgg taagcgagcc attatagcgg aaggaattca gatgatcagg aagcagcgcg
      421 ttcaagttga aatggctaac ggggagtgtg gtcgacgtgt cctgcgggat atgggagaaa
      481 atgggatcca gcgtctggtt gaaggagccc tcttgtaccc agcggcctac cacgagcaag
      541 tgcttgttcg cgtcctgaaa cacgagatgc atctccattg ggtatttgta gccattctcc
      601 agatgctcag caggcgcatg gaaatggaat tgggataacg tgtaagtatc gccgttcaca
      661 ctgagcgtgc tcgcgctcgg atcatcaaga tttgccctga ccgccgtttt gtccaccgca
      721 ggcgagccat tattgatgac cttgagtccg gcatcggaac tcaggttgaa attcagcttg
      781 ggaaggtttg ggttatagac tgtattgttg cttgtgatgt cgatcgggct ttgctccagc
      841 cctacagctt gggcagcagg cgcgccgatc acaaaaaggg cggaaacgag cggtactaac
      901 ggcagtaatc ggaaggaaga tttattcata ctatggaccc cttataaaat tggcttttga
      961 tatgggaggg ttgcataacg attttatgca atgtgaacgt accatgaacg atgtcaataa
     1021 agcgtggctt aaatgttaaa aaaacgtcaa gttcatccct taaaaaaacg tcgagctcat
     1081 tcgcaggggc ttatcagggt tatggcttta aatactcttg ggcatgccgc aaattttgaa
     1141 agactccagt cagattcttg attccgggtt atgccaccag ccatcggggg caataagcat
     1201 atctgcagcc ttggggcccc agcttccacg ttcgtagggg ataacgggat gatggttttt
     1261 aaggacgggt tcgacgacga tccaagcggc ctctacggcg tcttcgcgcg caaacagcgc
     1321 gccttcgcca ttcatggcat ccctcaacag ccgctcgtag ggggattccc tcccgaagtg
     1381 ttcttcgacc aggcagagtt cgcgctggtc tccgacaaat tcctttccgg tacgcttgac
     1441 acgtgccgca agtgcaatgg ctgatatggg cgaaagcctg aaccgaaggt aattcgcata
     1501 ctccgcttgc gacgctgaat cagcaaaaag cttttggggc ggcggcttta gctggaccag
     1561 aacctccgca gcggtctccg cgagaaattt ccccgaacgc aaataccatg gcacaccctc
     1621 ccaacgccat gaatcgataa acagccgcaa ggcgcagaac gtctccacat ccgaattctt
     1681 ggcgacacca ggttcgctac gataaccttg atactgcccg cgcaccaggt catccggttt
     1741 caaggggcgc atcgcctgga aaacgcttgc cttctcgccc tggaccgccc caaaaccttc
     1801 gaagggaggt ggttccatgg caagcagcgc cacgatctgg aagaggtggt tctggatcac
     1861 gtcgcgtagg caaccggcag actcataaaa cgcgccccgc tcctccaccc cgaagttttc
     1921 tgcaagcgtc aattgaacgc tggcaacgta gttgcgattc cagatcggct ccaggaacga
     1981 gttggcaaag cgaaaataaa ggatattcat gatcgcttcc ttgccgagga agtgatcaat
     2041 ccggaaaatc gcatcttcgg gaaagacgga cagtgcaatg cggttcagtt cgcgagcgga
     2101 agcaagatct cttccgaagg gcttttcaac gataaccctc gcaccatgag cgagtccggc
     2161 ggctccgaga tttttgataa ccgtctcgaa aagggatggg ggaatcgcaa ggtaatgaac
     2221 gggccgcttg acgttgccca atgcctgctt gagcttcttg aacgtaccgg gatcgttata
     2281 gtcgccgtcg atatacttga gcagggaaag gaggcgactc agcgcggcgg ggtcatcaat
     2341 tttttccgca tttgcatgtt taaccgcatc cgtggctctt tcccggagct gatcgatatt
     2401 ccagggcgtg gaggccacgc cgacgacggg cacgttgagc aagcccttct ttatcatcgc
     2461 atatagggca gggaatatct ttttatacgc caggtcgccc gtcacgccga acagcaccag
     2521 cgcgtccgat cgcgcccgtt tttgttcgga atcatcagtc tccgccatct caggctcctc
     2581 cttttttctc gtcatgccca ccgaagcgct tgcgcattgc tgaaagcacc tgagcggcga
     2641 aaaccgcgtt gcctcgtgat tcaaaacggc tgaacagcgc ggcggcgata acgggtacgg
     2701 gaacgccctc atcgatcgcg gccagcgttg tccagcggcc ctccccggag tcggatacat
     2761 ggcccgaaaa tgcactgagt ttggcgtctt cggccagcgc ctccgctgtc aggtccagca
     2821 gccatgaagc gacaatgctc ccccggcgcc agacctcggc gatctgggga atatcaaacg
     2881 cgtattgata gtattcgggg tcacgcaggg gagccgtttc cgcatccgta tcccgcttcc
     2941 ggtcaccggc gttggcgtta tgcagaatat tcaatccttc tgcataagcc gccatgagac
     3001 cgtactcgat gccgttgtga accattttca caaaatggcc agcgccgtgc ggcccgcagt
     3061 gaagatagcc ctgctcggca gtgccgtcgc tccgggtaag ggtggatgtc cggggcgctg
     3121 agtcgatacc gggggcgagc gcagcaaaga tcgggtcgag atgctgcaca atttcttttt
     3181 ctcctccaat catcaggcaa tacccgcgct cacggccagc cacgcctccg cttgttccaa
     3241 catccacata atgtacgccc tttgcgttca aatccgccgc gcgcctgata tcgtcccggt
     3301 aataagaatt gcccccatcc acgataatgt cgcctggact gacgagtgga aggagctccg
     3361 aaatcagctt gtcaaccacg gccgcgggca ccatcaacca tatcgcgcgc ggtttggaca
     3421 ggcgggagat gaaatccttc agggaggttg cgccttcggc acccgctgat tgcaactcct
     3481 gaacagcctc ggggcgtgtg tcaaacacca cgcactcgtt ccccgccttc atcaggcgag
     3541 ctgtcatgtt aacgcccatg cggcccaggc cgatcattcc gatttgcatc tcgatcctcc
     3601 ttcttcagtt aagagtcgaa tttcattcgt ataagtggag acgcgccagc tttagcagcg
     3661 acaagctcga ttttcatagg gggatgacag ataaggcctt ttacctgcat cccttggatt
     3721 ctcagtgtcg ttcaggaggc atggtgagga cctgccggag ccacctcgat gaatattctc
     3781 ttgacgcgcg ggtgttgctg ctggattgcc acttcgagcc gggaaacagc ctttgccaat
     3841 tcacttaccg ataacgaagg aacgaactga gcctcgatat tcaatagaac gtcctccgga
     3901 ccaaacacca tactcagaac ctggttcacg gccaccactg ccggttcatt gcgcgccagc
     3961 gagatcacgc tttccacgac ttccggcgtg gctgcttcgc ctaccagaag ccccttgctc
     4021 tccctgatca gtatgacgga aacaacgcat agcgtgacac caatcaggac cgatgctcct
     4081 ccatcgtaat agggattgct gaaacgatgc gcaagataga tgccgaaaaa cgctacggcg
     4141 agcccgatca gtgcggctgt atcttcgagc agtacggtca gtactgctgg gtcatagctg
     4201 cggcgcaagg tctgccatag cgtgtacttc cctttctcct tcaaaagcgc acgcattgcg
     4261 aaggtccatg agattccttc caacagcatc gacaccacga gtactacata ggcccagaac
     4321 gggtcttcga gcgggcgggg atttatgaca tgatccactc cctcgtaaat ggccattcct
     4381 ccaccaatcc caaagagcat tatggcgacg atgagactcc agaaatagag ttccttccca
     4441 taaccgaagg gatgctggcg atcagcgggc cgccggctgc ggttgactcc gaggagcagc
     4501 a
//
LOCUS       DASSHJ010000098         4575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1526145,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000098 DASSHJ010000000
VERSION     DASSHJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526145"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..561)
                     /locus_tag="VFI43_03955"
     CDS             complement(313..561)
                     /locus_tag="VFI43_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061314.1"
                     /translation="MSRLGREEIQGNRDLVEQQRLAIIAVGRQAEHRLSLLRKVFSEL
                     TSHFVEGRVVMRGHLSLSLSVGLFEGKERAIPLTQFSN"
     gene            919..1614
                     /locus_tag="VFI43_03960"
     CDS             919..1614
                     /locus_tag="VFI43_03960"
                     /inference="COORDINATES: protein motif:HMM:NF023560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spherulation-specific family 4 protein"
                     /protein_id="HET7061315.1"
                     /translation="MNLLVPAYFYPSNNSTYWDDLAAAAKAVGTTVILNPSTGPGTSI
                     DENYVSVVNKVREAGGKVIGYVHTTYGKRSRAEVTEEIDRYISYYHVDGFFVDQMTTD
                     TLDENIAYYQSIRDYIKGLGPLYSVTGNPGTMPDEVYLSHRLVDRVVVFEGTESSYAS
                     FLPSEWQSRYPKDKFAHLVYNANAQQMERAFSLSSDNHVGNLYVTGERTVNPYDALPD
                     YWDLEVQTASATH"
     gene            1780..2253
                     /locus_tag="VFI43_03965"
     CDS             1780..2253
                     /locus_tag="VFI43_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7061316.1"
                     /translation="MPLTSAGILPYRIRAGHLEVLLVHPGGPFWARKDLGSWSIAKGE
                     YEDGEDPLQAAQREFFEETGNSPLGPFLPLAQRKQPSGKLIDIWAVESDWNATDLVSN
                     TFSMEWPRGSGKICKFPEVDRAEWFDVAEAARRILPGQRGFLDELAQKLARSLPG"
     gene            complement(2347..2577)
                     /locus_tag="VFI43_03970"
     CDS             complement(2347..2577)
                     /locus_tag="VFI43_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061317.1"
                     /translation="MIKFVPYVRTIEGYIERQSYAVFNSPDSKPMPYVYEEELAGWPE
                     TKVFWASKTGPSLGLAPLANSSSLEQAPTDHP"
     gene            2822..4084
                     /gene="hemA"
                     /locus_tag="VFI43_03975"
     CDS             2822..4084
                     /gene="hemA"
                     /locus_tag="VFI43_03975"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET7061318.1"
                     /translation="MSSTQLFAFGINHQTAPLAVREQVAFPEDGMQHALQDLVAGRPV
                     REAAIVSTCNRTEVYCSTDKPDEAMRWLADFHHLQARELEPYLYKLPREKAVKHAFRV
                     ASGLDSMVLGEPQILGQLKNAVKSAEDAGTLGMLLHKLFQRTFFVAKEVRSSTEIGTN
                     SVSMAAASTRLAERIFGDINEQNVLFIGAGEMIELCASHFSARNPKRMTVANRTVDRA
                     QVLANRFNAHVITLNELPEQLALHDIVVTSTASTLPILGKGMLERAIKIRKHRPIFLV
                     DLAVPRDVEPEVAELDDVFLYSVDDLSEIVKEGLDSRQGAVAQAEAIIDSNVINFMHW
                     LESRQVVPTIRALRDQAEQYRRHELERAKKLLAKGEDPQKILESLSNGLTNKFLHIPS
                     YALNHAAADERNDMVDFINRLYHLHRPE"
     gene            4081..>4575
                     /locus_tag="VFI43_03980"
     CDS             4081..>4575
                     /locus_tag="VFI43_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HET7061319.1"
                     /translation="MNKSMAAKLTQLSVRLEELNRLLSSETVTRNIDKYRKLTRERAE
                     IAPVVELYDSYLQSERDIQTAQEMSAEVSADPEIRDFADAEMRSARERMAQIGVELQK
                     QLLPKDPNDERNIFLEIRAGTGGDESALFAADLFRMYARFAERQRWQVEVISQSPSEA
                     GGYKE"
BASE COUNT         1138 a         1175 c         1196 g         1066 t
ORIGIN      
        1 tccaggaatc cagcgaagta ccgttcttgt gatttgcaat aaccctgggt tcggaaaagc
       61 tctctacaaa tccaatagcg ttcaagttct cgaggctgta gaagtgtttt tgacggagag
      121 ctcgactatt tacggtgatt ctctcaaccg acatgctgcc cgtcaacgtg aagcactaag
      181 gagaaatgga aaatggaaaa cagcgaaagc caaaccagcg ctcgactgct ctgttctgaa
      241 aatctggtgc aggacttgct aaagcaagca gaaattgatc gcagctctct cagcgcagag
      301 aaaaggcagg ttctaattgc tgaattgcgt aagcgggatc gcacgttcct tgccttcgaa
      361 aaggcccacg ctcaggctga ggcttagatg gccgcgcatg acaacccggc cttctacaaa
      421 atgcgaggtt agctcggaga agacttttct gagcaggctg agacgatgct ccgcctgtcg
      481 gccgacggca ataatcgcca gcctctgttg ttcgaccagg tcgcggttgc cctggatttc
      541 ttctctcccg agtctggaca attggcgcct gcagttacgt taatgtcaac tgcaagtcct
      601 gcggcacctc tttcaccatc cgagccctat tttcgtaagg aatatcaaca attactcacc
      661 ccggctttcc agaagtcaac aattcaacta tcttatacaa gagagctatt ttctctttcg
      721 cgctgagctt tataacagaa gaatagaagt gacgagggac gagagcacct gactcccagg
      781 agttcaagtt gaagcgatgc ggctagtcaa gcgaggaagc gcaccgtcag ggcatattaa
      841 tctttctttg ccgtttcttt tgttacaaaa ggacagtggg aaatcgatct atttgcccct
      901 tcttaggaga cagtcgcaat gaatcttctc gttcccgcat atttttaccc ttcaaataac
      961 tcgacatact gggacgatct ggcggcggca gctaaagcag ttggaacaac ggtaatcctt
     1021 aaccccagta ccgggcctgg gacaagcatc gacgaaaatt atgtgtcagt tgtcaacaag
     1081 gttcgcgagg ccggtggcaa ggtgatcggt tacgtgcata ccacttatgg aaaacgttct
     1141 cgggcagaag tcaccgagga aattgacaga tacatcagtt actatcatgt tgacggtttt
     1201 ttcgtcgatc agatgactac ggatacgctt gatgaaaata tcgcttatta ccaatccatt
     1261 cgcgactaca tcaaagggct gggtccgctt tattccgtaa cagggaaccc cggtacaatg
     1321 cccgacgaag tttatttaag ccaccggctc gtcgataggg ttgtcgtgtt tgaaggcact
     1381 gaaagcagtt acgcaagttt tcttccctcg gagtggcaat ccaggtaccc aaaagacaaa
     1441 tttgcccacc ttgtgtataa cgccaacgca cagcaaatgg aaagggcatt tagcttatct
     1501 tccgacaacc atgtcggtaa cctgtatgta actggcgaaa ggaccgttaa tccttatgac
     1561 gcgcttcctg attactggga ccttgaagtc cagactgcat ctgcaaccca ttagtggggt
     1621 tggataaaaa taaccaccaa ctcgagcagc tcaaaacttc tccaaagttt cggaaaaaat
     1681 ctcagggcga cccacccggc tgcactcgcg tcgagcagga aaacgcgata gagccgcttg
     1741 ggccggccta aacattcatc gtttctctca tagagcatca tgcccctcac gagcgcaggc
     1801 attctcccct accgcatacg agccggacat cttgaggtcc tccttgtcca ccccgggggg
     1861 cctttctggg caaggaaaga tctaggttcc tggtccattg cgaagggtga gtacgaggat
     1921 ggtgaagatc cattacaagc tgcgcagagg gaattttttg aggaaaccgg aaacagtccg
     1981 ctcggccctt tccttccact tgcacaacgc aagcagccga gtggaaagct tatcgacatc
     2041 tgggcggtcg aaagcgactg gaatgccaca gatttggtct caaacacgtt cagcatggaa
     2101 tggccgaggg ggtcaggaaa aatctgcaaa tttccggagg ttgatcgcgc ggaatggttt
     2161 gatgttgcag aagctgctcg ccgcattctt cccgggcagc gcgggtttct tgatgagttg
     2221 gcccaaaaac tggcgcgaag ccttcccgga tgaaccatgg tatggccttt ggaaagttcg
     2281 catccctttc gaagccgctt ggatttccac agtcaacctg gactcttcgg aagccgttga
     2341 tcacgtttat gggtggtccg ttggggcttg ttccaaactc gaactattgg cgagcggtgc
     2401 gagaccaagg ctaggaccgg tcttgcttgc ccaaaagacc ttggtctcag gccagccggc
     2461 aagctcttct tcgtatacgt aaggcattgg cttgctgtcg ggagagttaa agacggcata
     2521 ggattgccgc tcgatataac cttcgatcgt tcgtacataa ggaacaaact tgatcatttg
     2581 accaccttta gtatagtgaa atacgcggac aatggtactt actgtttgtt acagaatatg
     2641 gaccacatgg acgagcttac atggacgagc gggagcggcg aagtatttcc cgcgatcaaa
     2701 aatgctgcca ggttgtcata cactgcaata tttcgctaaa ccagaaactc ttctaagcga
     2761 tggtattcaa aagcttgtga ggctataatc agcgaatttt cattgttgcc cccgttctat
     2821 cgtgtcttca acgcaactat ttgccttcgg cattaatcac caaaccgcgc cgcttgccgt
     2881 gcgcgagcag gtcgcatttc ccgaagatgg gatgcagcat gctttgcagg acttggttgc
     2941 gggtcgcccg gtaagggaag cggcgatagt ttcaacatgc aaccggaccg aagtttattg
     3001 cagtactgac aaacccgatg aggcgatgcg gtggctcgca gactttcatc atttgcaggc
     3061 gcgtgagctc gaaccgtatc tctacaagct gccgcgcgaa aaggcggtca agcacgcctt
     3121 ccgcgttgcc agcggcttgg attccatggt gctgggagag ccgcagatac tcggtcagtt
     3181 gaaaaacgcc gtgaaatcag ccgaggatgc gggcacgctt ggcatgctcc tgcacaagtt
     3241 gttccagcgc acctttttcg tcgccaagga agttcgcagt tccactgaaa tcggcacgaa
     3301 ctccgtctcg atggcagcgg cttccacccg tctggcggag aggatattcg gcgacatcaa
     3361 tgagcaaaat gtattgttca ttggcgccgg ggaaatgatc gagttatgcg ctagtcattt
     3421 ttcggcgcgc aaccctaagc gaatgacggt tgccaatcgc actgttgacc gcgcccaagt
     3481 acttgccaat cgtttcaacg cccatgttat aacgttgaac gagcttcccg agcaattggc
     3541 tttacacgat atcgtagtaa cctccactgc cagcacgctt cccatactcg gcaaggggat
     3601 gctggaacgc gcgatcaaga ttcgcaagca tcgaccaatc tttttggtgg atctggccgt
     3661 gccgcgtgac gtggagcctg aagtggcgga gctcgatgac gtgttccttt attccgtcga
     3721 tgacctatct gaaatcgtca aggaaggact cgattcgcgc caaggggcgg tagcgcaggc
     3781 tgaggcaatt atcgattcga atgtgatcaa cttcatgcac tggctcgagt cgcgccaagt
     3841 ggttcctacc attcgagcgc tgcgtgatca ggccgagcag tataggcgtc atgaactgga
     3901 gcgcgccaaa aagctcctgg ccaagggcga ggatcctcaa aagatacttg aatccctgag
     3961 caatgggctg accaacaagt tcttgcatat tccctcttac gcactcaatc acgctgctgc
     4021 ggatgagcgc aacgacatgg ttgatttcat caaccgcctg tatcacctgc atcgcccaga
     4081 atgaacaaaa gcatggctgc caagctcacg cagctcagcg tgcgcctgga ggaattgaac
     4141 cgtctgctga gcagtgaaac tgttacccgc aatatcgaca aatatcgcaa gcttacgcgt
     4201 gaacgcgcag agattgcgcc tgtggtcgag ctttatgact catatctgca aagcgagcgg
     4261 gatattcaga ctgcacagga aatgtcggct gaggtttccg ctgatccgga gattcgcgac
     4321 ttcgccgatg ctgaaatgcg cagcgctcga gaaaggatgg cgcaaatcgg agttgaattg
     4381 caaaagcaat tgctccccaa ggacccaaat gacgagcgca acatctttct cgaaatacgc
     4441 gcaggaacag gcggcgacga gtccgcgctg tttgcggcgg acctgttccg gatgtacgcg
     4501 cgctttgcag aacgccagcg ctggcaagtc gaagtcattt cacagagtcc ttccgaggcg
     4561 ggtggatata aggaa
//
LOCUS       DASSHJ010000099         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1110660,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000099 DASSHJ010000000
VERSION     DASSHJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110660"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..520
                     /locus_tag="VFI43_03985"
     CDS             242..520
                     /locus_tag="VFI43_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061320.1"
                     /translation="MNANLTLTFLASSDEKASPNTGGVGGMPGGMISLSANASSPGSA
                     VLWASVPYGNANSEITYGRLLAYDPENFVDRADGSLRIQTLWDSQDWR"
     gene            774..974
                     /locus_tag="VFI43_03990"
                     /pseudo
     CDS             774..974
                     /locus_tag="VFI43_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(951..1484)
                     /locus_tag="VFI43_03995"
     CDS             complement(951..1484)
                     /locus_tag="VFI43_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET7061321.1"
                     /translation="MSELVVVGFKDAEEADRVLLRLAKLKKEHLIDLEDAVVVIRDEA
                     GRVHLKQSIYPTVEGATTSFVLASGSLWGALIGLLFLHPFAGFVLGGALGLGAEALSG
                     SLADYGINDDFIKSLGETIPVNSSALFILVRKAQPERVLAELSGVKGKLLRTSLSPEQ
                     EKKLQEALADSTPRITA"
     gene            complement(1548..1898)
                     /locus_tag="VFI43_04000"
     CDS             complement(1548..1898)
                     /locus_tag="VFI43_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HET7061322.1"
                     /translation="MTLGERTLIPLEDIPESMNAALRGWVNYFHYRNSGRIFGKVKAH
                     AEERLRTQVMKRHKVKDRGTGLGQFGLGQFPSQKLYTFYGLYKVPTTADWKTAQVLAW
                     GTSKAACRKTACAA"
BASE COUNT          837 a          924 c          868 g          776 t
ORIGIN      
        1 aaaacgcgga tttcgctccg gccaaaatag ccagtaacta cgcacgggcc aaatacattg
       61 gctggttcac cttcttcccc ggttttgcgg tcagcccgac cccaactgat atgacccaac
      121 tcaacaagct cttcgatggc aaagcgcatc atcagcactc aacgcccggg cctacaaatc
      181 gcctatccac ggctggatgg tgttctgctg gggagagaac gcaagcctcc gcgcctggaa
      241 gatgaacgcg aaccttaccc tgactttcct ggcatcgagt gacgaaaagg cgtcgccaaa
      301 tacaggcggc gtgggcggta tgcccggcgg aatgatcagc ctgtcggcga acgcctctag
      361 ccccggctcg gccgtattat gggcatcagt accgtatggc aatgccaact ccgaaattac
      421 ctatggccgc ctgctcgcct atgatccgga gaacttcgtt gacagagccg atggttccct
      481 gcgcatccag acgctatggg acagccagga ctggcgctga gctatgtgtt taacaagttc
      541 ctgccgccaa ctcctgccaa tggcaggatc tatcttgcca actatgcggg gggcgtggac
      601 gtttatggtc attgaaagct ggcctatgag acaagagagg gcacggtctg gaggtcaatt
      661 ccatgattcg ctctctatcc ttgcgattga cttcatcccg ctcgtcctga tctggacgtt
      721 cagtacgaat aatctgtaat tgaccgggag ttggggaagc agaccgagga catgtgttcg
      781 gcatcatcaa atccgtcctg ggctttcgcc agtttctgct gcgcgggttg aacaaggtca
      841 gtggcaaatg gggcctggta tgcctggcct ggaatttgaa acgctggcta cgttgcgccc
      901 ttaaggtgca atgcgctttg cttattgcac cccatgtctc tacgtcccag ctatgccgtg
      961 atgcgaggtg ttgaatccgc gagggcttcc tgaagctttt tctcctgttc aggggaaagg
     1021 gatgttcgca ggagcttgcc tttaacgccg gagagctccg ccaaaactct ttccggttga
     1081 gccttgcgca cgagaatgaa aagcgccgac gagttgacgg ggatggtttc gcccaacgac
     1141 ttgatgaaat catcattgat gccgtagtcg gcgagagagc cggacagcgc ctctgccccg
     1201 aggccgagtg cgccccccaa tacaaagccg gcaaacggat gcaggaacag cagtccgatc
     1261 agggcccccc ataagctgcc ggaagcgaga acgaaactgg tcgtcgctcc ctccacagta
     1321 ggataaatgc tctgcttgag gtggaccctg ccggcctcgt cacggatgac gacaacggcg
     1381 tcctcaagat ctatcaggtg ttcctttttt aatttggcga gtctaagcaa aacacgatcg
     1441 gcttcttcgg catctttaaa acccactacc accagttcag acatattttt ctcctgtcac
     1501 gaatcaatag atagagtaga gaacagacgc tcgccggctc tccctcatca agccgcgcat
     1561 gcggttttcc tgcacgcggc tttcgatgtt ccccacgcca agacttgcgc tgttttccag
     1621 tccgctgttg tgggaacttt gtatagccca tagaacgtgt aaagcttttg gctgggaaat
     1681 tggcctaagc caaattggcc taagccggtt ccacgatcct tgaccttgtg tcgcttcatc
     1741 acttgcgttc gcaagcgttc ttcggcgtgc gcctttacct tgccaaatat tctgccggaa
     1801 ttgcgataat ggaagtagtt cacccagcca cgaagggcag cgttcatgct ctcaggaata
     1861 tcttccagcg gtatcagtgt gcgttctccc agggtcattg agttatgcgg tctttgattt
     1921 tcacaagaga tttcggtact ggacatatat tcgcatagcg tttgcccggt ttccaactct
     1981 tgatcaccct gattgcaaaa acccaagaaa ttgaagcttt ctttccgggc atcaacaacg
     2041 tggggacttg gtctcgtttt gcgtgagatc aagtccttcc aatacatgcc ctaccgttgc
     2101 caagggcaca tccactttac cctgcataac accacaaaat cgtcaatgta gcggacgaag
     2161 atgcgggtgg cagttcgcca gcagcggtga aatcacgctg ccttgcggaa tgtgccacgg
     2221 ctgttggcct tgccaccgcc ggttgttgcc gtcgaatcgg catcagccct taatccctat
     2281 attgcgcgtt atcgcgaaaa ggcaacccta ctctcaaaac gctgctggcg attcaagaca
     2341 gtcagctacg attatccgtt gaatctgaat ccgggcatca cgcacacgtt taaaatgaaa
     2401 gcatgatgat acgccattat catcaggaca tgcttggttt ggacttccgg ttgccgccga
     2461 acatcacagt aacaggccaa cacttgccag cggcaaaatg cacttttctg gtattcgcac
     2521 ccgatgcaac aaacgatgga cgatttgaag actacgcctg cctcatatat ccactatgct
     2581 cgccataaca tgccgaccta tattatcgac ccgaattggg cgacgcgcag tggcgcaccg
     2641 ctcggccaac atcgtcaggt gaggccgcag cgcagctcga tggaatgctg ggggccgaag
     2701 agtacaacgg cccgtcatcg acttgcgacg caagactgcc gccgcgcgcc gccaccggtc
     2761 agttaaagtt aattcttact agctcgagtt cgccgaacct cctttcacat tccattcgcc
     2821 tttcacattc cattcgcttt aaccaggctt caaccaaaat ttaaccaaga tttaaccgaa
     2881 atttaactgt ttcttcgctg gagagagcta tccttgaacc gtatagggga cggttcggaa
     2941 gaatcaatag aagaagaaca acctgccccc gacggaaata agccactcac actaagaggg
     3001 ataacagcag ggaaaccgct cggaactggc gaggctaacg taaaagcccg cgaaatgagg
     3061 aatgaaggga aaagccatct ttatccgttc ggaactcccg gaggaaggcc atggcattca
     3121 cattcattga tgacattctg atgagggttt agacagggca aattgagcgg gaaaaatcct
     3181 ggagctggct tgagtagcgg aacgtgaacg agcaccgcac ttctgtttag caaggtcctg
     3241 cttagtaatg attaccaaag gaagaagaga agaaacggat agtccgaagg tactaacaag
     3301 gtgggaaaac ggagaagttt ttgacaaaca ggagaagata tcatgccaat acgcggcact
     3361 aatagagacg atactctaaa cggcacatcg aaagcagata ccatc
//
LOCUS       DASSHJ010000100        10920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_130896, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000100 DASSHJ010000000
VERSION     DASSHJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10920
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130896"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFI43_04005"
     CDS             <1..1142
                     /locus_tag="VFI43_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7061323.1"
                     /translation="PFSVVDELSQIVEADQLISVRLATGYSRATCSFELAKRGRERAE
                     AALNTSRYVGAAPVPLRDYARALARQSIRQVQVNDEWIRHSLRHMVIGEKMLGQLGPA
                     FSSGRSIFFYGPPGTGKTSVAETLARALPGDVYIPQSVEVSGQIIRLFDPAIHASLEN
                     KNEEVPQLDLRMTLKHDPRWKRCRRPVVMVGGELTMDMLNLRYDTDARFYEAPVHMKA
                     GNGVFILDDFGRQQVEPRQLLNRWIIPLERGTDFPALHTGLKFEIPFDQITVFCTNMR
                     PADLVDEAFLRRIRHKIQVQNQTEGEFKEILRRVCDTRGVAYNTDATEYLLDNYYRKT
                     GRPLTGSHPRDLMEHIVDRARFIGRAPEFTPETVNLAAESYFVQM"
     gene            1548..3836
                     /locus_tag="VFI43_04010"
     CDS             1548..3836
                     /locus_tag="VFI43_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061324.1"
                     /translation="MARIDEIKALCDRLAPLGWRGLLRAVTRATLDISQSTPAALLAE
                     LTKNLPEINRGLPGFEDFAQEGNKAVAPAQPALSLLYHALASPLITRDHEGEPLQDFA
                     TTAELDLLEDFIFSLAPLLLPKFIEDSGGAPNVAVAVFSCEYRPASDTVDGHHADLTF
                     SRTGIARMGTARPRYHPILRGFWPEDEDNPNGIRVLPARFTAWLAVKKKGRDTRVSPI
                     LESSEGQASDEPGRIFLIPVHKLFSGKDCIEGLDLDVKLASRLFNLKIQRIHKALGVD
                     PLPDSFPYVIRDGEIADWSTDANLGPGWLVPTPRPRLVEAAMIDGKPLTFRVTPDFID
                     DFAAFEPSAAGVGRGAAPEYVHARTQVKGDRFLDLNDEMDVIGAMKKEPYDALHYVDF
                     SGEGWVQANIPVLTDRNIAFVAAYALVCAPDFFPACGQFEVSEWSRSKDVPAQFRGSL
                     WLIDPTPLSETRLPANLQLQDNPFTADDATITAVVSMGIPKGLPAIWPQQRDVLRASS
                     LPDDAAGVFAPGWDVGTDEKPGGNGVRHLAAYALGSPFPEDAKLCAALSTFWPAVAPD
                     VFRTFATPLEHTGGTVAPLTDEEIGQSGSLPWDGIRGPRVVTQDGKRYVELASFLNAD
                     YVRQVMQNRMSIRLTAQISAEDYQDRILAVSRAYSVVGDRGDIKETRRQWLVLSFRVI
                     TSADVELQRAQSEAHAILRGQIYRMELCKSPPVATREKISAYLHRFPLQDLCYFFTSP
                     DSQTVLIKREHDLRWASASSEG"
     gene            4321..4851
                     /locus_tag="VFI43_04015"
     CDS             4321..4851
                     /locus_tag="VFI43_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061325.1"
                     /translation="MRARWVFDGTGLRAWLARRLKLQADHSGPPLRVSFGWKDGSDPF
                     LEGQPLFRQHKGGWEWHAPLGNGRTAWVRLEQVVRRTKPDDGVHDLSGLDMTWRLHHL
                     SAGPGYFLLGDAAALLDPASSNGVLRALMSGMLAADLAARMLRGADECSAAHAYMNWI
                     EAIYHHQRKSLRQLYC"
     gene            complement(5004..5945)
                     /locus_tag="VFI43_04020"
     CDS             complement(5004..5945)
                     /locus_tag="VFI43_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7061326.1"
                     /translation="MNAIQIAYLAAIFIVVALAAFGAIRALTPRPTKNRLDRIGGAGS
                     IAEAGPETTLEQTIVSLATPLAKLALPDEGWEKSLLRLRFMHAGYRAKSVPMVYFAAK
                     TLLALVFPAAFLLYAGIGRLELSAHAALIVVLGLCACGYYLPNLILSRQIASRQRELF
                     ESFPDAIDLMTVCIEAGLGLDAAMVRVGEEIRLRSPTLADELHLVSLELRAGRTREQA
                     LRNLALRTGVEEIEALVSMLVQADRFGTSVAASLRVHSDTLRTARRLKAEEAAAKIAL
                     KLLFPLIFFIFPSLLLVLMGPAFINIYRIMLPAFGGQ"
     gene            complement(5963..6973)
                     /locus_tag="VFI43_04025"
     CDS             complement(5963..6973)
                     /locus_tag="VFI43_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7061327.1"
                     /translation="MIPRGGVFSGAVEMDTLYYLFLIFLFLAVVLFLEGAYLAWNAYK
                     GPEAKRIQERLQAVSAGWTSAETALMKQRLLSNSPAMDRFLLQVPRVHALDRLLQQSG
                     MSLMVTHFLGYTGIAAVGGMASGALLGLPSIGLMICAAAGSALPLFFVVSAKRKRLDK
                     IERQLPEAIDLMARALKAGHAFSGALQMVSTDGPEPVAAEFRVTFDEINFGVSAQDSL
                     MNLATRVPITDLRYFVIAVLIQRESGGNLAELLEKISGLIRDRLNLLGRVRVLSAEGR
                     MSAWILSCLPFATALMINFANPGFLAVLWTDPAGVAMVEGAAAMMAMGIFTMSRIIKI
                     RV"
     gene            complement(6948..8300)
                     /locus_tag="VFI43_04030"
     CDS             complement(6948..8300)
                     /locus_tag="VFI43_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET7061328.1"
                     /translation="MSIRERLQSVKQTATEQITRFSAAVDIPAFEELKALIHQKLLDR
                     MDLTIMESLPPERLLVEIKNLVERLLVEEGTPINEAERQCVVRDIQNEVLGLGPLEPL
                     LADSSISDILVNTHRQVYVERRGQLQLTDTHFASEAHLRKIIDRIVSRVGRRVDESSP
                     MVDARLPDGSRVNAIVPPLAIDGSLLSIRRFSVKPLKMEDLISYKSLTPEMAEIIGGL
                     VKAKCSILISGGTGSGKTTLLNIMSGYIPSTERIITIEDAAELQLQQPHVVRLETRPP
                     NVEGKGEISQRALVRNSLRMRPDRVIIGEVRGAEALDMLQAMNTGHEGSMATVHANTA
                     RDALARVENMVNMAGLNLPIKAVRQQITSAIWVVIQALRLTDGKRKVTSIQEITGMEG
                     DIITMQEIFTFDQTGLSPDGSVQGHFRATGIRPKFAERLKKYGVPLREDLFDPSRRSI
                     "
     gene            complement(8510..9670)
                     /locus_tag="VFI43_04035"
     CDS             complement(8510..9670)
                     /locus_tag="VFI43_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7061329.1"
                     /translation="MKITVIAEDWRCPNDIQKFLVDAGAPYPLVAVTGGVQQVVVVAE
                     QDLPDFLILQGEHFSSNELQILSHVSSRFPDLTVVMLCQDQSPEFLIKAMRAGVREVL
                     PVPLTQPVLIDAVERFQQRMALGRAPANSCKVLAFIPCKGGTGATFLATNIAYALAAL
                     EEKRVALFDFNLQFGDASLFIHDRPPINSIADVARQIQRLDGSFLASSMVQVLPNFSV
                     LAAPEDPEKAAEIKPEHINPLFQVAVKHYDFLVLDVGRELDAISLQALDRADMILPVM
                     QQTLPSVREAKRMLNVFRALGYSEEKLKLIVNRHEKTADISVSDIESTLNQKIFKVVP
                     NDYAVVTRAVNQGIPAIKLARRSVVAKSLQEIAHELARTAGQASLLRKLFSF"
     gene            complement(9797..10249)
                     /locus_tag="VFI43_04040"
     CDS             complement(9797..10249)
                     /locus_tag="VFI43_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HET7061330.1"
                     /translation="MKNARRNCEFSGLEARFLQRGVAAVEFALIASLVFILLFGIMEM
                     GRVLFYWNTAAETTRLGARLAVVCNKDAAAIKARMQSMLHLLSPANINIAYAPASCDI
                     NTCRLVTVSITGLTVSTFIPFVPLSLTMPSFSTTLPRESLDSANPDCH"
     gene            complement(10246..10725)
                     /locus_tag="VFI43_04045"
     CDS             complement(10246..10725)
                     /locus_tag="VFI43_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HET7061331.1"
                     /translation="MKCTSPRTLNPHELRGGVTLEFALLLVPLLFIAFGVIEYSRALH
                     QYNALVKSVRDAARYIAQRNPAESTTYSSALDDTRCLAVHGNTECAGPALVPGLTTDM
                     VSITPVSAATAEGTAIALVEVRIRGYSFAFAFNPLKLAGDNATVLLFGDIRAAMRQI"
BASE COUNT         2471 a         3100 c         3018 g         2331 t
ORIGIN      
        1 ttcccttcag cgttgtggat gaactatcgc agattgtcga agcagaccag ctgatttctg
       61 tccgcttggc gacggggtat agccgcgcga cctgcagctt cgagctggcc aagcggggac
      121 gagaacgtgc tgaagcagcc ctgaacacga gtcgttacgt cggtgcggcg cctgttccgc
      181 tgcgggatta cgcgcgagcc ctggcgcggc aaagcattcg acaggtccag gtgaatgacg
      241 agtggatccg tcattcgttg agacacatgg tcatcggaga aaaaatgctc ggccagctgg
      301 gccctgcctt cagttccgga cgctccattt tcttttacgg gcctccgggc acggggaaaa
      361 cgagtgttgc agaaaccctg gcaagggcat tgccggggga tgtctacatc cctcagtcag
      421 tcgaggtaag cggccagatc atccgcctgt tcgatccggc cattcatgca agcctcgaaa
      481 acaagaatga agaagtaccg cagctcgatt tgagaatgac cctcaagcac gacccgcgct
      541 ggaagaggtg ccgccggccg gtcgtaatgg tgggtggcga actgaccatg gacatgctca
      601 atctgcgcta cgacacggat gcgaggtttt atgaggcccc ggtgcacatg aaggccggaa
      661 atggcgtatt catcctcgat gactttggcc gccagcaggt ggagccacgt caactgctca
      721 accgatggat tattcccctg gagaggggta ccgattttcc ggcgctgcat accggcttga
      781 agttcgaaat accttttgac cagattacag tcttctgtac caacatgcgc cccgcagacc
      841 tggtcgatga agcatttctg cgccgtatcc gccacaagat ccaggttcag aaccagactg
      901 agggtgagtt caaggaaatt ctgcgccgcg tttgcgacac gcgtggcgtc gcctataaca
      961 ccgacgcaac ggaatatctg ctggacaact attaccggaa aacaggaaga ccgctcacag
     1021 gcagtcatcc gcgcgatctc atggagcata tcgttgaccg cgcccgcttc attgggcgtg
     1081 cgccggaatt cacgcccgaa acggtgaatc ttgctgcgga aagttatttt gtgcagatgt
     1141 aaaagacgta gaagggacgt agagagcgat catcaggcat cactaaagtc atttccccgg
     1201 cactgttaaa gggagcacga gaagcaatgc cgccagttga ttttttctac tcgagaggcg
     1261 tcttatcggg tactttcccc gctcagtcat gcaaggcgtc gctttacccg aactggcatt
     1321 ttctataccc ggcctgctcg acccctgaaa aaggaaaaat agaggcagag acaggaagga
     1381 atgaatatcg gcagggatag ctcgaatgcc ggaagcatct cttgcgcact tctttatagt
     1441 ccagaatagt ccagaatttg cctttccttg tctcatgcct gcttcttcct tattttctac
     1501 ctataaagga aaggagcacg atccatatca gaaatcgggg agggtaaatg gcacgaatcg
     1561 acgaaatcaa agcattatgt gaccggctcg ctccccttgg atggcgcggt ctgctccggg
     1621 ccgtgacgcg cgctacactg gatatttccc aatccacccc cgctgcgtta cttgcggagc
     1681 tgaccaagaa tctacccgaa atcaaccggg ggttgcctgg gttcgaagac ttcgcgcaag
     1741 agggtaacaa agctgtagca ccggctcaac cggcgttgag cctgctttac catgcactgg
     1801 ccagtccgct gatcacccgt gatcatgaag gagagccact ccaggatttt gctaccacag
     1861 cagagcttga ccttctcgaa gattttattt tcagccttgc gccgctgctg ctgccaaaat
     1921 ttatcgagga cagcggcggc gcccccaatg tagcagtcgc cgttttttcc tgcgaatatc
     1981 gccctgcctc ggataccgta gatggtcatc acgctgacct gactttttca cgtaccggaa
     2041 ttgcacgcat gggcactgca cggcctcgtt atcatcccat cctgagggga ttctggcccg
     2101 aggatgaaga caatcctaac ggcatacgtg tgctgccggc tcgctttacc gcatggctgg
     2161 cggtgaagaa gaaaggccgc gacacgcgcg tgtcgcccat acttgaaagc agcgaagggc
     2221 aggccagcga cgaacccggc aggatctttc tgattcccgt ccataagctc ttttccggca
     2281 aggattgtat cgaggggctg gatcttgacg tgaagctcgc ttcacgactg tttaacttga
     2341 agatacagcg gattcataaa gcgctcggag tggaccctct tccggacagt ttcccgtatg
     2401 taattcgaga cggtgaaata gctgactggt cgacagacgc caaccttggc cctggctggc
     2461 tggttcctac tcctcgaccc cggctggtgg aagcggcgat gatcgatggc aagcccctca
     2521 cattccgggt cacccctgat tttatagatg attttgctgc cttcgagcca tcagcggcgg
     2581 gagtcggtcg cggagcagcc cctgaatatg tccacgcacg cacacaagtg aaaggggata
     2641 gattccttga tctcaacgac gaaatggacg ttatcggtgc gatgaagaag gaaccttatg
     2701 acgcgcttca ttacgtcgat ttttcggggg aaggttgggt ccaggcaaat attccagtcc
     2761 tgaccgatcg caatatcgcc ttcgttgcgg cttacgcact cgtctgcgca ccagacttct
     2821 ttcctgcctg tggacaattc gaggtatccg aatggagcag aagcaaagac gtcccggctc
     2881 agttccgtgg aagtctctgg ttaatcgatc ccactccgtt aagcgaaacc cggctccccg
     2941 ccaaccttca gctccaggac aaccctttca ccgctgacga cgctaccatc accgccgttg
     3001 tcagcatggg aattccaaaa ggtctgcctg cgatatggcc gcagcagcga gacgtgctgc
     3061 gcgcatcctc cttgccagat gatgctgccg gtgtcttcgc acccggatgg gatgtaggaa
     3121 cggatgagaa accagggggt aatggcgtga ggcatctggc cgcctacgct ttgggtagtc
     3181 catttccgga agacgccaag ctctgtgctg ccctcagcac cttctggccg gccgtcgcgc
     3241 cggacgtttt tcggaccttc gcgacaccac ttgaacatac cggtggcacc gttgcgcctc
     3301 ttaccgacga ggaaataggg caatccggtt ccctaccttg ggatggcatc cgcggtccca
     3361 gagtcgtgac gcaagacgga aagcgctacg tggagctcgc gtcgttcctg aatgccgact
     3421 atgtccggca agtaatgcag aacagaatga gcatccggct gactgcacag atcagtgccg
     3481 aagactatca ggatcggata ctggccgtga gccgggctta cagcgtcgtc ggcgatcggg
     3541 gcgacatcaa ggaaacgcga aggcaatggc tggtgctctc cttccgggtc atcacttccg
     3601 cagatgtcga gttgcaacga gcccagagcg aagctcatgc gattttgcgc gggcagattt
     3661 acaggatgga attatgcaaa agccctccgg tagccactcg ggaaaaaata agcgcttact
     3721 tgcatcgctt tccgttgcag gatttatgct atttcttcac aagcccggat tcacagacgg
     3781 tgcttatcaa gcgcgagcat gacctccgtt gggcgagcgc ttcgagtgaa gggtaagctc
     3841 tggagataag aggcggtcct acccttatct atctgaactg catgcctgtc ctccagagtg
     3901 acattctgat catcggcgca gggcccgcgg ggtgcgcggc cgcgatctcc gcccggcaac
     3961 ggggcttgac tgtcatactc atcgacgctc gcgcttgcgc gtcgcgcacc gggtgagtcc
     4021 ctccaccctg gtatcgaacc gctgttcaag aagctagggg tctggaatga cataataacc
     4081 gccggctttc accggcatcg gggcatttgg cgtgaaatcg aggattcacg cacatttgcg
     4141 ccatatggct ccgatagtaa tgaagagtgg ctcggcttcc aggctgaccg ccacactctg
     4201 caaagcatcc tgcaaagagc ggcaagcgaa gccggtgcgg ttttcaagcc agccacttcg
     4261 ccaagcgaca tcatcctcca cgacgatcgg attaaaggaa ttgtctgtgt cgaagtcgag
     4321 atgcgtgccc gatgggtttt cgatggaacc ggcctacgcg cgtggctggc acgcagattg
     4381 aaacttcagg cggatcattc gggaccgccg ctgcgcgtga gttttggctg gaaggatggc
     4441 agcgatccgt tcctggaagg gcaacccttg tttcgccagc ataaaggtgg ttgggagtgg
     4501 catgctcccc ttggaaatgg caggaccgca tgggtcaggt tggaacaagt cgtgaggcgt
     4561 acaaaaccgg atgatggcgt gcacgatctt tctggtctcg acatgacatg gcgtctccat
     4621 cacctttccg ccggacccgg ctatttcctg cttggggatg ccgcggcact gctcgaccca
     4681 gcctcatcga atggcgtttt gcgggcgctg atgagcggaa tgctcgctgc cgatcttgct
     4741 gcccgtatgc tccgcggcgc cgatgaatgt tcggcagcgc atgcttatat gaactggatc
     4801 gaagcaatat atcatcatca aaggaaaagc ttgcgtcaac tctattgttg acctgaatta
     4861 gataagcgaa atccccggcg gaaaatacag atccggccgc ggtgcgcccc gctgacgagg
     4921 atgccgattg acacatgcgc cgccacgcaa gcagaacttt atggtagctc tgcgaagcgc
     4981 gaacgccatt gacctgagca caatcattgg ccgccaaaag ccggcagcat tatgcgataa
     5041 atattgatga aggcgggccc catcagtacg agcagcaatg acggaaagat gaagaagatc
     5101 aacgggaaga gcagcttaag cgcgattttg gctgctgctt cttccgcttt gagccggcga
     5161 gcggtgcgca gggtatccga atggacgcgt agcgacgccg cgacgctcgt gccgaaacga
     5221 tcagcctgaa ccagcatcga aaccagcgct tcaatttcct cgactccagt gcgcaaggca
     5281 agattgcgca aggcctgttc ccgcgttctg cccgcacgaa gctccaggct taccaggtga
     5341 agctcgtccg cgagtgttgg gcttcttaga cgaatttcct ccccgaccct gaccatcgcc
     5401 gcatcgagcc ccaacccggc ctcgatgcaa acggtcatga gatcgatggc atctggaaaa
     5461 ctttcgaaca gctcacgttg acgtgatgcg atctggcggc tcagtatcag gttgggaaga
     5521 tagtaaccac aagcgcacaa tccaagcacg acgatcagag cagcgtgagc actgagttcc
     5581 aggcgtccaa tcccggcgta gagcaggaaa gcagcaggaa atacgagagc cagaagcgtc
     5641 ttggctgcga agtagaccat cgggacagat tttgcacgat aaccggcgtg catgaagcgc
     5701 agccgcaaca aggatttctc ccatccttca tcgggcagtg ccagcttggc cagaggcgtg
     5761 gccaaactca caatggtttg ctccaaagtg gtttcagggc ctgcctctgc tatactgcct
     5821 gcgccgccaa tccgatccag gcggtttttc gtaggtcggg gcgtcagcgc gcggattgcg
     5881 ccaaaggcag ccagcgccac aacgatgaag attgccgcca gatatgcaat ttgtatcgca
     5941 ttcataaccg tgttatttcc attcacacgc ggatcttgat gatgcgcgac atcgtaaaga
     6001 tacccattgc catcattgcg gccgctcctt ccaccattgc cacgccggcc gggtccgtcc
     6061 acagcaccgc gagaaagccg gggtttgcga aattgatcat caacgccgtg gcaaacggaa
     6121 ggcatgaaag aatccaggct gacatgcggc cttcagcgga aagtacacgg acccgtccaa
     6181 gcaagttaag cctgtcacgg atcaatccac tgattttttc gagcaattca gccaagttgc
     6241 cgccactttc acgttggatg agcacagcaa ttacaaagta gcgtaaatcc gtgattggga
     6301 ccctggtggc gagattcatc agcgaatcct gcgcggagac gccgaaattg atttcgtcga
     6361 aggtaacccg aaactcggcc gcgactggct caggcccatc ggtggatacc atctgcaatg
     6421 cgccggaaaa ggcatgacct gccttcaagg cgcgcgccat caaatcgatc gcttccggca
     6481 attgccgctc gatcttgtcc aggcgcttgc gtttcgcgct caccacgaaa aacaaaggca
     6541 acgcgcttcc cgctgccgcg caaatcatca ggccgatgga gggaagcccg agcaatgccc
     6601 cgctcgccat tccgcctacg gcggcaatac ccgtataacc gagaaagtgt gtcaccatga
     6661 gagacatccc cgattgctgc agcaaccggt caagcgcgtg aacgcgcggc acctgtaaca
     6721 gaaagcgatc catagcggga gagttgctca aaagccgctg cttcatcagt gcggtttcgg
     6781 cgctcgtcca acctgcggaa accgcctgca gccgttcctg gatgcgcttc gcctctggcc
     6841 ctttgtatgc gttccatgcc aggtaggctc cttccaggaa cagcaccacg gcgagaaaca
     6901 aaaatatgag gaaaaggtaa tagagcgtgt ccatttccac cgcgccgcta aatactccgc
     6961 cgcgagggat caaacaagtc ctcccgcaac ggcacgccat acttcttcaa ccgttctgca
     7021 aacttcggac ggatgccggt cgcacggaaa tgcccctgta cgctgccgtc gggcgacagt
     7081 ccggtctggt caaaggtaaa aatttcctgc atggtgataa tgtcaccttc cattcccgtg
     7141 atttcctgga tgctggtcac cttgcgcttg ccatctgtga ggcgcagcgc ctggatgacc
     7201 acccagatcg cggaagtaat ctgctggcgc accgccttga tcggcaaatt cagcccagcc
     7261 atgttcacca tgttttcgac cctggccagc gcgtctctcg ccgtgttggc gtgcacggtc
     7321 gccatcgagc cctcgtgacc ggtattcatg gcctggagca tatccaatgc ttcagcgcca
     7381 cgaacttcgc cgatgatcac cctgtcgggg cgcatgcgaa gactattgcg caccagcgcg
     7441 cgctgggaaa tttcgccctt tccctcgaca ttgggaggac gtgtctccag gcgtacgaca
     7501 tgaggctgct gcaactgtaa ctccgcggca tcctcgatgg taataatcct ttccgtggaa
     7561 ggaatgtagc cggacatgat gttaagaagg gtcgtcttgc cgctaccggt tccgccggaa
     7621 ataaggatgc tgcacttcgc cttgaccagg ccgccgataa tctcggccat ttcgggggtc
     7681 agcgatttat aactgatcag atcctccatc ttgagcggtt tgacggaaaa gcgacggatg
     7741 gaaagcagcg atccatcgat cgccagtggg gggacgatgg catttacgcg cgagccatcg
     7801 ggcaggcgcg cgtccaccat cgggctggat tcatccacgc gccgccccac acgcgaaacg
     7861 atccgatcga taatctttct taaatgggct tcgctggcga aatgggtatc ggtcaattgc
     7921 agttgaccac ggcgctctac gtagacttga cgatgcgtat tcacaagaat atccgagatc
     7981 gaggaatccg ccagcagagg ctcgaggggc cccaacccca gcacctcgtt ctgaatgtcc
     8041 cgcacaacgc attggcgttc cgcctcgttg atcggcgtgc cttcctcgac cagcagtctt
     8101 tccaccagat tcttgatttc caccagcaac cgctctggcg gaaggctttc cattatcgtg
     8161 aggtccatcc ggtcgagcag tttttggtgg atgagtgctt ttaattcctc gaaggcaggg
     8221 atatcgactg ccgctgaaaa ccgggtgatc tgttcggttg cagtctgctt cacgctttga
     8281 agccgctctc ttatcgacat ggctggaatt ccgcaaaatc ggtcaagggg ccgcaatcca
     8341 aatgagggat taccggctgg ggaaagaaag ggcaggagtg accaggtagc atgcaggacc
     8401 ggaggacccg tgcaaccaaa gccgcaccgg ctgcattgga aagggctcga gtgcgttggc
     8461 catcccgcgg ctcgaagctc attcgaatct catttcactg ccttcctcgt caaaagctaa
     8521 acaatttccg gagcaggctt gcctggccgg ccgtgcgagc cagctcatgt gcgatttcct
     8581 gcaaggactt cgctacaacg ctgcgccgtg cgagcttgat cgccgggatc ccctgattca
     8641 ctgcccgggt gacgactgca taatcatttg gcacaacctt gaaaatcttc tgattgagcg
     8701 tgctctcgat gtcactcacg ctgatgtcgg cagttttttc atgccgattc acgatcagct
     8761 taagcttttc ctctgaatag ccaagcgcgc ggaaaacatt cagcattcgt ttggcctccc
     8821 tgacagaagg aagcgtctgc tgcatgaccg gcagtatcat atccgcccgg tccagcgctt
     8881 gcagcgagat tgcatcgagc tcgcgcccta catcaagaac caggaagtcg tagtgtttta
     8941 cggccacctg gaatagagga ttgatatgct ccggttttat ttccgcggct ttttccggat
     9001 cttccggcgc ggcgagtaca ctgaaattgg gtaatacctg caccatgctt gaggccagga
     9061 atgatccatc gagccgctga atctgccggg caacatccgc aatcgaattt atgggcggac
     9121 ggtcgtgtat aaacagcgat gcatcgccaa actgcagatt gaaatcgaat agcgcaacgc
     9181 gcttctcctc gagcgcagcc agggcatatg cgatattggt tgcaaggaag gttgctccag
     9241 taccgccttt gcaaggaata aatgccaata ccttgcatga attggcaggc gcgcgtccca
     9301 gggccatcct ttgctgaaaa cgctcgacgg catcgatcag cacaggttgc gtgagcggaa
     9361 cgggtaaaac ttcacggacc ccggcgcgca tcgccttgat aaggaattct ggcgattgat
     9421 cctggcacag catgacaact gtaagatccg gaaatcgtga gctcacatgg ctgagtattt
     9481 gcagttcgtt ggaactgaag tgctcacctt gcagtatcag gaaatcaggc agatcctgct
     9541 cagcgaccac gacaacctgc tgcaccccgc cggtaacggc aacgagcggg tagggggcgc
     9601 ctgcatcgac cagaaatttc tggatgtcgt tcggacaccg ccagtcttca gcgattacag
     9661 taatcttcaa tttcttgttt tcttttattc gttaccccgg attcaagctg gcagtcccgc
     9721 tcctgcatgc acccatcctg taaccagcaa ctcggtagca tcttcttcgc gggtcaatgc
     9781 gcggcttgta ggtagttcag tggcaatcgg gattcgcgct gtcgaggctt tcgcgcggca
     9841 aagttgtgga aaacgaaggc attgtcaaac ttaacggcac gaagggaata aaggttgaaa
     9901 cagtcaaccc cgtaatgctg accgtaacga ggcggcaagt attgatatcg caacttgccg
     9961 gtgcgtaggc gatattaatg ttggcaggcg acagcaggtg gagcatgctc tgcatgcgcg
    10021 ccttgattgc ggcagcatcc ttattgcaca caaccgccag acgcgctccc aaccgagttg
    10081 tttcagccgc cgtattccaa tagaacagga cacgtcccat ctccatgatg ccaaacagca
    10141 ggatgaaaac gagagatgca atcaacgcga attcgacggc cgcgacaccg cgttgcaaaa
    10201 aacgtgcttc gagtcctgaa aattcacaat tgcggcgggc attcttcata tttgcctcat
    10261 cgccgcacga atatcgccaa ataaaagaac tgtcgcgttg tctcctgcca gcttcagtgg
    10321 attgaatgca aaagcgaagg aataccccct gatcctgact tcaaccaggg cgatcgccgt
    10381 gccttctgct gtcgcagcgg aaactggcgt aatgctgacc atatcggtcg tcagcccggg
    10441 aacaagggca gggcctgcgc attccgtgtt gccatgaaca gccaggcacc gggtatcatc
    10501 caatgctgac gagtaagttg ttgactccgc cgggttgcgc tgcgcgatat atcgcgccgc
    10561 gtctcgcacc gacttgacga gcgcattgta ttggtgcagc gcacggctgt attcaatcac
    10621 gccaaatgcg atgaacagca agggaaccag cagcaaggca aattccagcg ttaccccccc
    10681 gcgcagctca tgcggattca aagtcctggg actcgtgcac ttcatccgtt ttccgcttcc
    10741 gattgtcatc gaacgagcac gggaacgagc gggccgccgc tgccgggtcc cgcagggaca
    10801 ccggatgttg cgcaggggtt gccagtgacg tcggatcggc ctagatattc gagatacatc
    10861 cgagttgagc ccatcccact gccgccctgg ctattattga tgggatgcag catcaacacg
//
LOCUS       DASSHJ010000101         6066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_296645, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000101 DASSHJ010000000
VERSION     DASSHJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296645"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VFI43_04050"
     CDS             <1..1123
                     /locus_tag="VFI43_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7061332.1"
                     /translation="LAVTSPAGIIYSTNITPDKEFGIGNYTYEDFSRAVREGIAKDGR
                     NLYPAMPYPSFTGISDEDTRALYAYFMKGVKAVNQKPPQTALPFPFNQRWVFRFWNAA
                     FTKNVRFQPSPDRDKRWNRGAYLVRALGHCGACHTPRGFAYQEKAYSESSSEYLSGAV
                     IDNWFAGNLTANRTSGLGRWSEADISEFLRTGHNGHTVAFGSMVLVVENSTQHLDKED
                     VDAIAHYLKTLGARKGRSSYRPRTSISKISAEIGMEGFERPGQGIYAGFCAKCHQDSG
                     TGKAPEIPRLAGSPMVLSENASSLIRLMLEGGKGPVTKAGPKPKKMPAYARELSSREI
                     AEVLTFVRNSWGNSASAVTTRDVALLREILENQVQATQD"
     gene            complement(1194..4457)
                     /locus_tag="VFI43_04055"
     CDS             complement(1194..4457)
                     /locus_tag="VFI43_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7061333.1"
                     /translation="MQGIRSALKRAPKKRVPEICKHGRLFEPILSRPTIAILAFAIFI
                     SLTVVGWSIVQELQERNENTRFEKALVEVTQKIEYHFSGYEQVLKGALGLLLASESVS
                     RNEWRTYVNALRIDDNYPGIHGIGFAKYISPSQLAAHIAEVRAEGFGSYKVWPESPRK
                     EYTSILYLEPFSGRNLRAFGYDMFSNPVRRAAMARARDIGEAALSGKIQLVQETHEDI
                     QAGILMYLPYYYAANIPRTVDERRASLAGYVYTPLRMDDFVGAVLRSELDILDLRIFA
                     GEAMVESSLLFDSKKSQPGKPGIADIPKFKRVISISLYGQTWAIEASSRPAFEEAIKS
                     YEPFLVLLGGILVSILTAMVSFVLSANKEKALALGRVNKKLLLAIEEQQATTLELSNA
                     KLRTERILESITEAFYTLDREWRFTYVNKEAENLLRRKREDLLGRVVWEEFEEAAGTA
                     LDHEFHRALIENVSVTFEEFYIPLGKWLEVHAYPSEEGLAIYFRDITARKHFEQARQE
                     ADSRLRQQASLLDKARDAIIVHGIDRRIQFWNQGAERLYGWTSEDVAGKSIEMLYENL
                     AAFNEATRLLLNSGEWRGEIQQRRKDGSVLLVEAHWSLVRNDDGRGQSVFAINTDITH
                     RKAAEKEILHLAFYDSLTGLPNRQLLLDRLRHALAVSTRNHGTGALLFIDLDNFKLLN
                     DTLGHDLGDLLLQQVALRLSSSVRESDTVGRLGGDEFVVILEADFSNGHDEGLARIRI
                     ICERIRSAFNQSFNLGAYEHNITPSIGIALFNDQLLTTNELLKRADLAMYQAKASGRN
                     AMCFFDPDMQAETNARAALESDLRQSWERKEFILHYQPQVDSRGIMGAEALVRWQHPH
                     RGLLPPSEFISRTEEIGLILPLGGWVLETACSQLAIWSLQPETAQLNLAVNVSPRQFC
                     QPDFVEQIISILDRTGANPKRLKLELTESILIRNMEETIEKMMALKAKGVGFALDDFG
                     VGYSSLYYLKRLPLDWLKIDRSFVRDVLTDNNNATIVRAIVLLAKSMGLAVIAEGVET
                     LAQKDFLATQGCSTYQGYLFSRPLPLDQFEELVHGRNQYRQSQVGRVTKTGGVCKIN"
     gene            complement(4890..5711)
                     /locus_tag="VFI43_04060"
     CDS             complement(4890..5711)
                     /locus_tag="VFI43_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7061334.1"
                     /translation="MDILYWLSPWEDSALVVAGTGIAALWFWRGSSSVKPALQQKVFF
                     WTGLACVYLVSHTQLDYYAEHQFFIHRLQHLVLHHLGPMLIVLSSPSVTLLAGMPRYG
                     RQLCHRMAEWKPFRYCGQALCNPFIAVLLFSGLVGLWLVPSVHFTVMIDWRLYRLMNW
                     SMFINGLVFWGLVLNREALLSLRLSPGTRIGMMLAVIPPQIVLGALIFFTDRELYPIY
                     TICGRAIGGVSAIADQQIGGIILWIHGAMMSAIGILIVVRKELMLEEVRERRQPG"
     gene            complement(5749..>6066)
                     /locus_tag="VFI43_04065"
     CDS             complement(5749..>6066)
                     /locus_tag="VFI43_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7061335.1"
                     /translation="LQRYIAQFDSERAVGLAGTAADIERLTKQYRAAYRPRSRISETA
                     DIEHGDAIYIFDAQRRARLLATSSDPDEHLMKDLRRLMQEAYQERGVRERAGDREHLP
                     RPD"
BASE COUNT         1500 a         1632 c         1525 g         1409 t
ORIGIN      
        1 cttggccgtt acatcgccgg ccggaatcat ttattcgacc aacattacgc cggataagga
       61 gtttggcatt ggcaactata cctatgaaga tttcagcagg gctgttcgcg agggaatagc
      121 caaggatgga agaaatcttt acccggcgat gccctatcct tcattcacag gtatctcgga
      181 cgaggacacc cgtgcccttt atgcctattt catgaagggc gtgaaggctg tcaaccagaa
      241 acctccgcaa accgccttgc ctttcccttt caaccagcgc tgggtctttc gattctggaa
      301 tgccgcgttc acgaagaatg tccgtttcca gcccagtccc gatcgcgata aaaggtggaa
      361 tcggggggcc tatctggtgc gagcactggg acattgcggg gcttgtcaca ctccgcgtgg
      421 ttttgcctac caggagaaag cgtattctga atcatcctcc gaatacctga gcggggcagt
      481 catcgataac tggtttgcag gtaatctgac ggcgaatcgc acatccggac tgggtagatg
      541 gagcgaggcc gacatttcag agtttctgcg caccggtcat aatggacata ccgtggcatt
      601 cggcagcatg gtgctggtgg tcgaaaacag tacccagcat ctggacaagg aagacgtgga
      661 tgcaatagcc cactatctga agacattggg agcccgaaaa ggaaggtctt cgtatcgccc
      721 tcgaacaagc atcagtaaaa tctcggctga gataggaatg gagggatttg agcggccggg
      781 ccagggaatc tatgcagggt tctgtgccaa atgccaccag gacagtggca ccggcaaggc
      841 gccggaaatt cccaggctgg ccggcagtcc gatggtcctc tcggagaatg cctcttcatt
      901 gatacggctc atgcttgagg gtggcaaggg accagtcacc aaagcgggac ctaaaccgaa
      961 gaaaatgccg gcatatgcaa gggaattgtc cagccgggaa atcgccgagg tcctcacatt
     1021 tgtccgaaat agctggggaa actccgcctc tgcggtaaca acccgggacg tagctctctt
     1081 aagagagatt ctggaaaacc aggtgcaggc gacgcaggat taaagaacgt ggagacgtag
     1141 actcatgacg caaagccatg acggtgcgta ttcgaacaat ccagccggaa ggctcaattt
     1201 attttgcata ccccacccgt tttggttact cgtccaactt gcgattgccg gtactggttc
     1261 cttccatgca ccaactcctc gaattgatct aacggcaggg gacggctgaa caggtagccc
     1321 tgataggtac tgcatccctg tgtagcaaga aagtcctttt gtgccagagt ttccaccccc
     1381 tcggcaatta ccgccaaccc catgcttttt gcgaggagca caatggcgcg gacaatcgta
     1441 gcattattat tgtcagtcaa cacatctctc acaaacgacc gatcgatttt caaccagtcc
     1501 agaggcaggc gtttcaggta gtagagcgag gaatagccaa caccaaagtc atccagggcg
     1561 aaaccaacac ccttggcttt cagggccatc attttctcaa ttgtttcctc catatttcgt
     1621 atcaatatac tctccgtaag ctcaagcttg agtctttttg gattggcacc ggtacgatca
     1681 agtatcgaga ttatttgctc aacgaaatcg ggttggcaaa actggcgggg gctgacattc
     1741 actgcgagat tgagttgggc tgtctctggc tgaagcgacc agattgccag ctggctacat
     1801 gcggtttcca gcacccaccc gcctaaaggg agaatcagtc caatttcctc cgtccgggaa
     1861 ataaattccg atggaggcaa aagtccgcgg tgagggtgct gccaacgcac cagcgcttcg
     1921 gcgcccataa tcccgcggct atccacctgg ggctgatagt ggagaataaa ctcctttcgc
     1981 tcccagcttt ggcgtaagtc ggactccaga gcagctcgag cattcgtttc tgcttgcata
     2041 tccgggtcga aaaaacacat ggcgtttcgt cccgaagctt ttgcttgata catcgccaga
     2101 tccgcccgct tcagcagttc gtttgtcgtg agcaattgat cgttaaatag tgcgatgccg
     2161 atactcggag tgatgttatg ttcatatgcg ccgagattaa aagattgatt gaaggcgctg
     2221 cgaatccttt cacaaatgat ccttatcctg gcaaggcctt catcatgacc gttgctaaaa
     2281 tccgcttcca gtatcaccac aaattcgtcg ccgcccaaac ggccgacagt gtcgctttcc
     2341 cggacagagg agctcaggcg gagggcgacc tgctgcagca acaagtcccc caagtcatgg
     2401 ccgagcgtat cattaagcag tttaaagtta tcgagatcta tgaacagtag cgctccggta
     2461 ccatggttac gagtactcac tgccagggca tggcgtagcc gatccagcaa gagctgccgg
     2521 ttgggtaaac ccgtcaggga atcatagaag gcgaggtgca gaatttcctt ctccgctgcc
     2581 ttgcggtgag taatatcggt attgatcgcg aagacagact ggccccgccc gtcgtcattt
     2641 ctgaccagac tccaatgcgc ctccacaagc aatacacttc cgtctttacg acgctgctgg
     2701 atttcacctc tccattcccc cgagttcagc agcaggcgag ttgcttcgtt gaaggctgcg
     2761 aggttttcat acagcatctc tattgattta ccagctacat cttcggacgt ccatccgtag
     2821 agccgctcag ccccctgatt ccagaactgg atacgacgat cgataccatg cacgataatt
     2881 gcgtccctcg ccttatcgag caaggaagct tgttggcgga ggcgagaatc cgcttcctgc
     2941 cgtgcctgct caaaatgctt tcgtgcggta atatcgcgaa aataaatggc aagaccttct
     3001 tccgatggat aggcatgtac ttcaagccat ttgccaagag gaatgtagaa ttcttcaaac
     3061 gttaccgaaa cattctcgat gagcgcccgg tggaattcat gatccagggc ggttcctgcc
     3121 gcctcctcaa attcctccca aaccaccctg ccaagaaggt cttcccgttt ccgccgtagc
     3181 aaattttccg cttccttatt cacataggtg aagcgccatt cccggtcgag agtgtaaaaa
     3241 gcctcggtga tactctccag aatccgctct gtccgaagct tggcatttga aagttcgaga
     3301 gttgtggctt gctgttcctc tatggcgagc agtagttttt tatttacgcg tcccagtgcc
     3361 agagctttct ctttgttcgc cgaaagaaca aaagaaacca tggcggtcaa aatactgacc
     3421 aggatcccac ccaacaggac aagaaagggc tcgtaacttt ttatggcttc ttcaaatgca
     3481 ggacgcgaag atgcttcgat tgcccaagtt tgaccgtaca gagatataga tatgacccgc
     3541 ttgaatttag gtatgtcagc tatacccggc tttccaggct ggcttttttt gctgtcaaaa
     3601 agaagagaac tttctaccat tgcctcgccg gcaaaaattc tcaagtcaag gatatcaagc
     3661 tctgaccgaa gaacagctcc gacgaagtca tccatcctca acggcgtata tacataaccc
     3721 gcaagagatg cccgtctttc atctaccgtt cgcgggatat ttgcagcata ataataggga
     3781 agatacataa gaatgcctgc ctgaatatct tcgtgggttt cctgcacgag ttgaattttg
     3841 cccgatagag cggcttctcc aatatcgcgg gcacgtgcca tcgcggcgcg ccgaacagga
     3901 tttgaaaaca tatcgtatcc aaaagcccgc aggtttcttc cagaaaacgg ctcgagatat
     3961 aaaatagagg tatattcttt tcgcggactc tccggccaga ctttatagga tccaaatcct
     4021 tccgcacgta cttctgcaat atgagcagcg agttgtgagg gactgatata tttagcgaaa
     4081 ccaatcccat ggattcctgg atagttatcg tctattcgta aggcgttaac gtatgtgcgc
     4141 cattcatttc gtgaaaccga ctcagaggca agcaggagtc ccagcgcgcc tttcaatacc
     4201 tgctcatatc ctgaaaagtg atactctatt ttctgggtaa cctctaccag tgctttctca
     4261 aatcgcgtat tttcgtttcg ctcctggagc tcttgaacga tcgaccaacc gacgaccgta
     4321 agtgatataa aaattgcaaa cgccaggatc gcgatagtcg gccgagagag tatgggctcg
     4381 aagaggcggc catgtttgca gatttctggt accctctttt ttggtgccct cttcaaagcg
     4441 ctgcggatcc cttgcatata aaaataaagc ttttgaccct cagctcattg ctcggctcga
     4501 gggggccaga ctttgtgtgg aagtgtagaa cgaacatgac gaaagggagt cagcgacgca
     4561 attcgcccgt cggaacgcgc cgtcgcctgc ttcaagaacc cctggaattt gctctctgta
     4621 aaacgcttct tcgatccgtt ctcctctcta aaaacgaact gaattccaga tgacagggct
     4681 acggggagcc catcatatca atgccgggat ttaatggtta accggataag atcgctaaaa
     4741 ccgttcgttt ttccaggttt aggtcgatca ttgatggttt gactcggagg ctgcttgatg
     4801 atgaaagcgg agggtaaccg cgaactcggc acattgaaaa cctccgaaaa agtcacgaag
     4861 aataatcacc ggaaggggga aaagctagct taccctggct gccgcctctc tcgcacttct
     4921 tccagcatca gttccttgcg aaccacgata aggatgccga tggcgctcat catcgcgccg
     4981 tgaatccaga gaattattcc gccgatctgc tgatcggcaa tggcgctcac gccgccaatc
     5041 gcccgtccac agatcgtgta gatgggatag agctctcgat ctgtaaaaaa gatcaatgcc
     5101 ccgagaacga tttgaggcgg gatcaccgcc agcatcatgc cgatgcgcgt ccctggcgac
     5161 aggcgcagcg ataataatgc ctcccggtta aggacaagcc cccagaaaac caatccgttg
     5221 atgaacatgc tccagttcat caagcgatat aaacgccagt cgatcattac cgtgaaatgc
     5281 acggaaggca ccagccataa accgaccagc ccgctaaaca acaagaccgc aatgaaggga
     5341 ttgcatagcg cctgcccgca ataacggaag ggcttccact ctgccattcg gtggcacaat
     5401 tgccgcccgt accgcggcat gcccgcgaga agggtcactg acggactgct caaaacgata
     5461 agcatcggcc caaggtgatg caacacgaga tgctggagcc ggtgaatgaa aaactgatgc
     5521 tcggcgtaat aatcgagttg cgtgtgcgaa accaggtaaa cacatgcaag tccggtccag
     5581 aaaaatactt tttgctgaag tgcgggtttg acggagctac tgccgcgcca gaaccataaa
     5641 gccgctattc cggttccagc tacgaccaga gcagagtctt cccacgggga aagccagtac
     5701 aagatgtcca tgatcggcta taaggtgaag tctctcctgc ccgtcctttc agtctggtcg
     5761 cggcaaatgc tctctgtctc cggctctctc ccgaaccccg cgctcctgat acgcttcctg
     5821 cataaggcgc cgcaaatcct tcatcaggtg ctcgtccgga tctgaagagg tggccagcag
     5881 gcgcgcgcgg cgttgtgcat cgaaaatgta tatcgcgtcg ccatgctcga tatccgccgt
     5941 ttcgctaatt ctggagcgcg gccggtaggc tgcccgatac tgcttggtga gccgttcgat
     6001 gtctgctgcc gttcccgcca gcccgactgc acgctctgaa tcaaactgcg cgatataacg
     6061 ctgcag
//
LOCUS       DASSHJ010000102        14986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1571364,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000102 DASSHJ010000000
VERSION     DASSHJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571364"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..3397)
                     /locus_tag="VFI43_04070"
     CDS             complement(272..3397)
                     /locus_tag="VFI43_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET7061336.1"
                     /translation="MLSRIIEWSARNVFLVLLGTVFLVGFGIYASQRTPLDAIPDLSD
                     VQVIIYTEYPGQAPQVVEDQVTYPLSTAMLAVPNSRVVRGLSVFGASFIYVIFEDDTD
                     IYWARTRVLEYLNFATARMPRGVTPTLGPDATGVGWVYQYVVLSARHTLAELRTLQDW
                     FMRYQLTKAQGVAEVASVGGFVKTYQVTVEPRKLQAYGISLGTVAAVVRASNRDVGGR
                     VVEMAETEYVVRGRGYLRGISDIENLVLRAAEGTPVLIRDVARVEFVPDERRGIAELD
                     GEGEVVAGIAVARYGQNALDVIDNLKGKIVEIGSGLPEGVSLTAIYDRSDLIYRAIEN
                     LKRILIEESIIVAAVCIVFLMHARSALVAIIMLPIGVLVALGAMHLLGLNSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKHLERAPPDKPRLQVIIGACREVGPALFFSLLIITVSF
                     LPVFTLESQEGRMFAPLAYTKTFAMAGAALLSITLVPALMVLFIRGKIMPERKNPLNR
                     LLIRIYRPIIAWVMRWKKLTIVAATLVLAITVIPATRLGSEFMPALNEGSLLYMPITL
                     PSVSVTKSAELLQTQDKIIKSFPEVASVFGKAGRANTATDPAPLEMAETIINLKPESE
                     WRAGMTLDRLITQMDLALQLPGVSNAWTMPIKNRIDMLATGIRTPVGIKVFGKDLGEI
                     EDLATRIEAVVKTIPGTTSAYAERTTGGYYLDIEPDRIELARYGLAIGDLLETISLAL
                     GGETLTTTVEGRERFSVNIRYPRELRQNPSAIAAEVLVPIKNGSMIPLGQLASVKTVK
                     AAPSIRTENALLSAYIFVDIRNRDVGSYVAEAQKAVVEQVRFPPGYYATWSGEFEYME
                     RAKQKLKVVIPLTVAIIFVLIYLNFRRFTETLIVMLSVPFSVVGGVWLMSLLGYNLSV
                     AAGVGFIALAGVAAETGIVMLIYLDHAWKTITARRAAEGQRPTVNDLYVAVMEGAVER
                     VRPKVMTVVAIMAGLLPIMWENGAGSEVMRRIAAPMIGGMVSSTVLTLIVIPAVYALV
                     KGWHLSKSTMA"
     gene            complement(3399..5111)
                     /locus_tag="VFI43_04075"
     CDS             complement(3399..5111)
                     /locus_tag="VFI43_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7061337.1"
                     /translation="MKYRGKRFNPFTVASLAIAVLGMALGSGYWLGTHAPHKDAGVTG
                     VSSLATPSEAEKMGKKGSQVEKGGKDDKGGKDGRGKILYYRHPMGQPDTSPVPRKDSM
                     GMDYLPVYEGEEAALETPLVTITPEKIQKLGVKTELAATRQLVRTVRAVATIQADERR
                     LHTVTPKFEGWIQTLYVNTTGQTVRKGQPLMEVYSPELDSAQHEYLIAKRGLEAVDEG
                     TSEVKAGMHRLAESALQRLRNWDIAEIDLRALEEKGEIKRYLVLRSRTNGVITEKPAT
                     QGIRFMPGDVLYKIADLSSVWVLADVFEQDLAMLRLGQAATIRIDAYAGRGFNGKVTF
                     IYPTIAPETRTAKVRIELPNPSGVLKPAMFGAVEFATPHGKDKVLAIPESSVLDTGTR
                     QLVLIARGEGRFEPRTVKLGFHADRYVEVLEGLAAGEAVVVSANFLIDAESNLKAAMT
                     AFSPPALAPANHPTAPFNASADTPGLASAGHRGTGRVEAVDWTRATVTINHEPIASLG
                     WPAMNMSFRVSDPSLLNSLKPGQQVDFEIVEKPPGEYIVTRIRPAQPKESAGHRDIGS
                     HRRH"
     gene            complement(5108..6505)
                     /locus_tag="VFI43_04080"
     CDS             complement(5108..6505)
                     /locus_tag="VFI43_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7061338.1"
                     /translation="MFQETIRSATVRRAAIPRVLMAHFVACVLLSQNVQSEIPHIPPG
                     ESASNTPEKFAAAPISAVPGQLGALVGEALENNPEIRAALHEREAAQHRVSPAQALDD
                     PVLEAGVINAPLASSTFNREDMTMKMIGLSQRLPFPGKRGLRKDVASRDAEAIGYGYQ
                     ETVNRVVRDLKVAYLDLGLTLEMTRLVKKNKLILEEFQHIAEDHYAVGRGNQSDILKA
                     QTQVSRMTDELFRLAREQPVQEADLIRTLSRNPGAAAPTPEPPRLIEQKLDMSELREK
                     AMAGRPQLLALKSIVVRSDKALDLARKNYYPDLDVRLAYGQRDNTLDGGRRPDMVSLT
                     VAINLPVWRESKLAPRVAEAVAFREQAESLYQAQRNEVAASLRQQAAVAEQNLKSAQL
                     YQTAILPQARLAVEAALAAYRVNRVDFLTLLDSQMTVFNYEISRTTAIAGYNKALAEI
                     DLLVGKAPLPEWKPM"
     gene            complement(6685..7044)
                     /locus_tag="VFI43_04085"
     CDS             complement(6685..7044)
                     /locus_tag="VFI43_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061339.1"
                     /translation="MALTLAALPLQGALAAIMPLCAQAGQALHLSTADEPSIHAAPTP
                     CSQHDASNGSLAQQTGEDQESFNLPCDGAVCHISGGGLPSATAPLSLFGGFSFITSFN
                     SRFTSSILPQPHRPPLA"
     gene            7336..7965
                     /locus_tag="VFI43_04090"
     CDS             7336..7965
                     /locus_tag="VFI43_04090"
                     /inference="COORDINATES: protein motif:HMM:NF022439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HET7061340.1"
                     /translation="MTAAIICTALPASVHASGPHVHGTATLEIAIDDAVVHVNLSSPL
                     DSLLGFEHAPRNEKERQAVRSMASKLHQVDNVLVFTPQAQCRLESAKLESSLLSSELL
                     MSSFDKGKADGADAAKQGTPQIRPQTRLPVLSSATASQAASADEHHDEHAELEGTWSF
                     RCATPGALAGAEVRLFHAFPGLRRLDVAIAGPKGQRSAKLSPASRQLKW"
     gene            7959..8729
                     /locus_tag="VFI43_04095"
     CDS             7959..8729
                     /locus_tag="VFI43_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7061341.1"
                     /translation="MVVSVPNLASHESGIKQFAVEIKNLVFQWPGRNGFRLSIPDFRV
                     QRGERVFLQGASGSGKSTLLGLLGGVLVPQPCRLRVLDTDLTALSSGARDRFRVDHLG
                     FLFQQFNLVPYLSVRENVLLPCRFSRLRRQRALETVNSSGTAPGQPDALGRSADHLLA
                     RFGLGAALARQPANALSVGQQQRVAAARALIGHPELIIADEPTSALDADRQAEFLDVL
                     LERCADESVTLIFVSHDERLAGHFTRRIHLNEISHHTE"
     gene            complement(9015..9359)
                     /locus_tag="VFI43_04100"
     CDS             complement(9015..9359)
                     /locus_tag="VFI43_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061342.1"
                     /translation="MVRLFYAFLALLILGSHVPAALAADASVTISSPQNGAKFSPRTE
                     VDVVYEATLGSNGNHVHLYVDGDEPVVLHAMKGSHSVGKLTVGLHKICINVVNKAHAP
                     VGAQACVNVTVE"
     gene            complement(9511..9981)
                     /locus_tag="VFI43_04105"
     CDS             complement(9511..9981)
                     /locus_tag="VFI43_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HET7061343.1"
                     /translation="MSGARAKHELKTERVYEEPEAGNDRGILVDRLWPRGLTKEKANV
                     DLWLKDIAPSTELRKWFGHAPEKWRSFRERYQTEFGHRPNQLKSIGSEVKEGTVTLVY
                     GARDQVHNEAVVLKRFLEKESLRHPEGGSACLNSDLSILSGIRADSHGSSSTVR"
     gene            complement(9978..10700)
                     /locus_tag="VFI43_04110"
     CDS             complement(9978..10700)
                     /locus_tag="VFI43_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7061344.1"
                     /translation="MTIYKVILKGREEVASGTMAFRFGKPEGFSYKPGQWADFTLIDP
                     PETDAEGNTRGFSLASAPYEDDLMVATRMRDTAFKRVLKVIPLGTELKMEGPGGSFTL
                     HNNAAIPAVFLSGGIGVTPVRSIVLQTIHDQVAREIFVFDSNNRPEDAAFLDELMRPH
                     EKNPHYTFIGTMTEMGKSSQEWQGEVGFIAKAMLLKYINELTLPIFYIAGPQGMVAAM
                     RKALNESGVDDDNIRTEEFSGY"
     gene            complement(10965..12092)
                     /locus_tag="VFI43_04115"
     CDS             complement(10965..12092)
                     /locus_tag="VFI43_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET7061345.1"
                     /translation="MAVPADHSPLHPLQEDFEDFFENSRCGFLTMNSRGEILRSNSRL
                     AGWLGCATENLKGLRFSELLTIGGKIYCETHLFPLLRMQGFFEEVALELACQGDERLP
                     VLVNAYERRDADSKPQFIRVTVFKATHRRLYEENLRYTRTIAETRLADEQAISVLREQ
                     FIAVLGHDLRNPLGAIMGGAELLVSSPLSERDTGLINMMRESAARMAELIDNILDFAR
                     GRMGGGMMLNRQPVLLEPVLRHVVDELRTAWPKRAIEAEFRLLDPVDCDSARLSQILS
                     NLLANALTHGSPEGPVRVRAFLERDLFELSVTNSGRPIPPEALEKLFQPFTRQDASSS
                     QNGLGLGLYIASEIARAHKGELTVSSNAEETCFTFRMPASG"
     gene            complement(12093..12881)
                     /locus_tag="VFI43_04120"
     CDS             complement(12093..12881)
                     /locus_tag="VFI43_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7061346.1"
                     /translation="MSPLKRNNVIVRGKGSTPMMFAHGFGCDQNMWRFVAPAFANSYQ
                     TILFDHVGAGRSDLTAYSREKYDTLEGYAEDIIEIARELRLKDAVFVGHSVGAMMGIM
                     AAAKAPDIFKALVLVAPSPSYINQGDYIGGFTRAQVEEIVESLDSNHMGWSMTVAPIL
                     MGCPDCKKLGEELVNSFCRTDPEIARQFARTTFLSDCRSILADVAIPALILQCSNDAI
                     APQVVGEYMHRQMPNSKLVLMEASGHCPNLSAPEETIAAMKAFL"
     gene            complement(13135..13746)
                     /locus_tag="VFI43_04125"
     CDS             complement(13135..13746)
                     /locus_tag="VFI43_04125"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7061347.1"
                     /translation="MQMSFGAMEMDARVGRDSALLKVHALIDWEGLRVELLELCKREA
                     NRAERQKSVDPLTMFKAVLLGQWHSLSDLKLEEALRVRIDFMYFCGLSLANDVPDEAL
                     LYRFRSFLITTHKLGGLLGLINAQLQAHGLMVKHAYGAVIDATLVQLADRSKCSAIAE
                     PNAVNEEGSIPDGVTSALPQLSNSAAVGNADLDATWIKKGTYI"
     gene            14153..14323
                     /locus_tag="VFI43_04130"
     CDS             14153..14323
                     /locus_tag="VFI43_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061348.1"
                     /translation="MSDPATQDRAAGAIMGAFIGDALGVGPHWCYDLAVLRCDYGQWI
                     TTYADPKPGSIP"
     gene            14292..14966
                     /locus_tag="VFI43_04135"
     CDS             14292..14966
                     /locus_tag="VFI43_04135"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET7061349.1"
                     /translation="MPIPSRVRYHSDLKAGQFSQTGIILRFMLRSLIERGSYDEADFC
                     RSMDEEIFPLLNGMPVNGPGYTQPSIRDTWRKRVQQNQPWGQMGGEADTTEAIGRTLA
                     LAVRYAFQPEELAATISSNTRLTQIDDIAVTLTVAYGAVLSLLVQAQAQQEPFGQAHA
                     IIKGGKTAFPHDNTYRPAAAPDGLSAEKRALRLSECPPHALICRDGRDGPGYTDRACM
                     ESLRRL"
BASE COUNT         3248 a         4245 c         4062 g         3431 t
ORIGIN      
        1 catccctgtt tacgcgaaat aaaagaaaga aacggcgtta atcaggagtc tctgggtaca
       61 agagggctgg gcaatacacg tctttcccca ggatctgcgc agcgatggtg agttagatga
      121 agacattttt tgccgctcgt ccgtcgtgta agttaatgaa atgtatttat agacaggagc
      181 ttatgtgatt gtcttaactt atgaaattaa cgtgaaacat acaaaaatct ttaggtggat
      241 tatgcaatgc gcaagcactg gttgctgggt actatgccat cgtacttttg cttaaatgcc
      301 atcccttgac aagagcataa acggcgggga tcacgatcaa ggtaagcacc gtggaagaaa
      361 ccattccccc gatcattggt gctgcaatgc gcctcatgac ttccgagccg gcaccgttct
      421 cccacatgat ggggagcagg ccggccatga ttgcgacgac tgtcatgact ttgggacgca
      481 cacgctcgac agcgccttcc atcacggcga cgtacaaatc attaacagtt ggtctctgac
      541 cttccgccgc gcgcctcgca gtgattgttt tccaggcatg gtcaagatag atcagcatga
      601 cgatacctgt ttccgccgcg actccggcaa gcgcgatgaa gcccaccccc gccgcgacgc
      661 taaggttgta gccaagcagc gacatcagcc atactccacc cactacggaa aaaggcaccg
      721 acagcatgac aataagtgtt tcggtaaacc gtctgaaatt caggtagatc aggacaaaaa
      781 tgattgcgac cgtaagcggt atcacgacct tcagtttttg cttggcgcgt tccatgtact
      841 cgaactcccc gctccaggtt gcgtagtagc ccggtggaaa cctgacctgt tccaccacag
      901 ctttctgggc ctctgccacg tagctgccaa cgtcgcggtt acggatgtcg acaaagatat
      961 aggccgacag caatgcattc tcagtgcgaa tgctgggtgc cgctttaact gttttgacgc
     1021 ttgcgagctg tcccagcggg atcatgcttc catttttgat ggggaccagg acttccgcgg
     1081 cgatggcgga ggggttttgc cgcagttcac gcggatagcg aatgttgacg ctgaagcgct
     1141 cccttccctc caccgtagtg gtcaacgtct cgccccccag agcgagcgaa attgtttcca
     1201 gcaggtcgcc gatcgcaagg ccataacggg caagctcgat gcggtcgggt tcgatatcga
     1261 gatagtagcc acctgtcgtc cgctcggcat aagcgctcgt cgttcccgga attgttttca
     1321 ctacggcttc gattcgtgtg gcgaggtctt ctatttcacc cagatccttg ccgaatacct
     1381 tgattccaac aggggtacgg atgcctgtgg cgagcatgtc gatgcgattc ttgatcggca
     1441 tggtccacgc gttgctgacc cccggcagct ggagcgccag atccatttgg gtgatcagcc
     1501 tgtcgagcgt catccctgcg cgccactcgc tttctggttt caggttgatg atggtctcgg
     1561 ccatctccag cggggccggg tccgtcgcgg tatttgcgcg ccctgccttt ccgaaaacgg
     1621 aggcgacctc cggaaagctc ttgatgatct tgtcctgggt ttgcaacagc tccgcggatt
     1681 tggtcactga cacggagggc aaggtgatgg gcatatagag caggctaccc tcattcagcg
     1741 ccggcatgaa ttcgctgccc agcctggttg ctggtatcac cgtgatcgcc aggacgagcg
     1801 ttgcggcaac gatggtgagc ttcttccagc gcatgaccca ggcaatgatg ggtcggtaaa
     1861 tccgtatcag gagccgattg agggggtttt tccgctcagg catgatcttg ccccgaatga
     1921 agagaaccat cagggccggc accagcgtga tggacagcaa cgctgcgcct gccattgcga
     1981 aggtcttggt gtaagcgagc ggggcgaaca tccgcccttc ctgcgattcc agggtaaata
     2041 cgggcaggaa cgaaactgtg atgataagaa gactgaagaa taaggccggt ccgacttctc
     2101 ggcaggcccc gatgatgact tgaagccttg gtttgtctgg cggagcgcgt tcgaggtgct
     2161 tgtgcgcatt ttcgatcatc acgatcgcag catccaccat ggcgccgata gcaatggcga
     2221 tgccacccag actcatgatg tttgaattga gcccaaggag atgcatggcc cccaaggcaa
     2281 cgagtacgcc aatgggcagc atgatgattg cgacgagcgc gctccgggca tgcatgagga
     2341 acacgatgca gactgccgcc acgatgatgc tttcctcgat caggatacgc tttaggttct
     2401 cgatagcacg gtaaatcagg tctgagcgat cgtagatcgc tgtcaatgac acaccttccg
     2461 gcaggccgct acctatctcg acaatctttc ccttgaggtt gtcaatgacg tccagcgcat
     2521 tttgtccgta acgcgccact gcgatgcctg caaccacctc gccttctccg tccagttctg
     2581 cgattccccg ccgctcgtct ggcacaaatt cgactcgcgc gacatcgcgg atcagtactg
     2641 gcgtgccttc cgcagccctg agcacaaggt tctcgatgtc cgagatgccg cgcaggtagc
     2701 ccctgccgcg caccacgtat tcggtttcgg ccatttcaac gaccctgccg cccacgtcgc
     2761 gattgctcgc ccggaccact gcggcgaccg tgccgagcga gattccataa gcctgtagct
     2821 tgcgcggctc gacagtgacc tggtaggttt tcacgaatcc gccgacgctt gccacctccg
     2881 ccactccctg cgccttggta agctgataac gcatgaacca gtcctgcagg gtgcgcagct
     2941 ctgcgagcgt atgccgggcc gacaggacga cgtattggta tacccagcca acgcctgttg
     3001 catccggtcc cagcgttggc gtgacgcccc gaggcatgcg cgccgttgca aaattgaggt
     3061 attcgagtac gcgagtgcgc gcccagtaaa tgtcggtatc gtcctcgaaa atgacataga
     3121 tgaacgaggc gccgaatact gacaggccgc gcacaaccct ggagttgggc actgccagca
     3181 tggcggtgct caggggataa gtcacctggt cctcgactac ttgcggggcc tgccctggat
     3241 attcggtgta gatgatgacc tgcacatccg aaagatcggg aatcgcatcc agcggcgtac
     3301 gctgggaagc atagattcca aagccgacca gaaagactgt tcccaacagc acgagaaata
     3361 cgttgcgggc agaccattcg atgatccggc tcaacatgtc aatgcctccg gtggctgccg
     3421 atgtcgcgat gaccggcgct ttccttcggc tgtgcgggcc tgattcgagt gacgatatat
     3481 tcacctggtg gcttttcgac gatctcgaaa tcgacctgtt gtccgggttt cagggaattc
     3541 agcaatgagg ggtcgctaac ccggaaactc atgttcatgg cgggccaacc aaggctggca
     3601 attggctcgt ggttaatcgt aacggttgct cgcgtccaat ccaccgcttc gacacggcca
     3661 gttccacgat ggcctgcaga ggctaggcct ggggtgtcag cggaggcatt gaagggggcg
     3721 gtaggatgat ttgcaggggc cagagcgggc gggctaaagg cggtcatcgc tgccttgaga
     3781 ttgctctctg catcgatcaa aaaattggcg ctgacaacga ccgcttctcc ggctgcaagc
     3841 ccttccagca cttcgacata ccggtcggca tgaaacccca gttttaccgt gcgaggttca
     3901 aacctgcctt ctccgcgggc gattaatacg agttggcgtg ttccggtatc aagcacggaa
     3961 gattcaggta tggcaagcac cttatccttg ccatggggcg tggcgaactc cacggcgccg
     4021 aacatggccg gtttaagcac gccactgggg tttggcagct cgatacgcac cttggctgtg
     4081 cgcgtctcgg gcgctatggt tggatagatg aatgtaactt tgccgttgaa ccctcgcccg
     4141 gcatacgcat caattctgat tgtcgcagcc tgcccgagcc gcaacatcgc gagatcctgt
     4201 tcaaacacat ccgcgagcac ccacacgctg gatagatccg cgattttgta cagcacatct
     4261 cccggcatga agcgtattcc ctgcgtggca ggcttttccg taatgacccc attcgtacgc
     4321 gagcgcaaaa ccaggtagcg tttgatttca cccttttcct ccagggcccg gagatcgatc
     4381 tccgctatgt cccagttgcg cagcctctgc aaagcgcttt ccgccaaccg gtgcatcccc
     4441 gcctttactt cagacgtgcc ctcgtcaact gcttccaggc ctcgcttggc gatcaggtat
     4501 tcatgttggg cactgtcgag ttccgggctg tatacctcca tgagcggctg cccttttcgt
     4561 actgtttggc cggtagtatt gacgtacagc gtttgtatcc agccttcgaa cttcggtgtc
     4621 acggtatgca gccgccgttc gtccgcctgg atcgttgcca cggcgcgaac ggtgcgaacc
     4681 agctggcgcg tagctgcaag ctctgtcttg actcccagct tttgaatttt ctcgggggtg
     4741 atcgttacca gaggcgtttc caaggcagcc tcttcaccct cgtataccgg gagataatcc
     4801 atgcccatcg agtctttcct gggaacgggg gaagtgtcgg gttgacccat cgggtggcgg
     4861 taataaagga ttttgcccct accgtccttg ccgcccttat cgtccttgcc gcccttttcc
     4921 acctgactac cttttttacc catcttttcc gcctcgctcg gagtagccag actggatacg
     4981 cccgtgaccc cggcatcttt gtggggcgca tgtgtgccga gccagtaccc tgaccccagt
     5041 gccatgccaa gcactgcaat agcgagtgaa gcaacagtga atggattgaa tcgcttgccc
     5101 cggtacttca catcggtttc cactcgggta acggagcttt gccgaccagc agatcgattt
     5161 ctgcaagcgc cttgttgtag cccgcgatcg ctgtcgtacg gctgatctca taattaaata
     5221 cggtcatctg gctgtcgagc agcgtcagga aatcaacccg gttgacccga taggctgcca
     5281 gtgccgcctc aaccgccaag cgcgcctggg gcaggatggc ggtttggtat agctgtgccg
     5341 atttaaggtt ttgctccgct acggcggcct gctggcgcaa gctcgccgcg acctcatttc
     5401 gttgtgcctg atacaggctc tcggcctgct ccctgaacgc cacggcctcg gcaacgcgcg
     5461 gcgccagctt gctttcacgc cacaccggca gattgatggc caccgtgagg ctgaccatgt
     5521 ccggccgccg cccgccatcc agcgtgttgt cccgctgacc gtaagccagg cgaacgtcga
     5581 gatccgggta gtagttttta cgtgccagat ccaaggcttt atcgcttcgc acaacgatgc
     5641 tcttcagcgc cagcaattgc ggacgtccag ccattgcctt ttctcgaagc tccgacatgt
     5701 ccagcttttg ctcgatcaat cgaggcggtt cgggggttgg tgcagcggca ccaggattgc
     5761 ggctcagcgt gcggataaga tccgcttcct gcaccggctg ctcgcgcgcg agcctgaaca
     5821 attcgtcggt catcctggag acttgagtct gcgccttcag tatgtccgac tggtttcccc
     5881 ggccgacagc gtagtggtct tcggctatgt gctggaactc ttccaggatg agcttgttct
     5941 ttttgacgag acgcgtcatt tcaagcgtca gcccgaggtc cagataagcc accttgaggt
     6001 cgcgaaccac gcggttgacg gtttcctggt aaccgtaacc aattgcctcg gcatccctgg
     6061 acgcgacatc cttgcgcaaa ccccgttttc cagggaatgg tagacgctgc gacaggccga
     6121 tcattttcat ggtcatgtct tcgcgattga acgtggacga cgcaagtggc gcattgatga
     6181 cgcccgcctc cagcaccggg tcgtcgagtg cctgggcggg agatacccgg tgctgcgccg
     6241 cttcgcgctc atgcagcgca gcccggattt ccgggttgtt ttccagcgct tcccccacca
     6301 acgcacccaa ctggccggga accgcggaga tgggagccgc agcaaacttt tcaggagtat
     6361 tcgacgcact ctcacccggg ggaatatggg gaatttctga ttgcacgttt tgggaaagca
     6421 gtacgcatgc aacaaaatga gccatcagta cgcgtggtat ggcggcgcga cgaaccgtcg
     6481 cactccggat cgtttcctgg aacatcctga tctcctctgg aatgggagtg aaactttagc
     6541 aggaggcata tccaccgcag agcgggggca gccggttgct gtttttttgc caaggccaag
     6601 cgaagcagga ctggcaattg cggttgaccc gccggcaacc ggccgaacga ccgatgcagc
     6661 aggcagattt atactggggg aaacctaggc gagtggagga cggtgaggct gaggaagaat
     6721 ggacgaagta aagcgggagt tgaaggacgt gatgaacgaa aagcccccga agagactcaa
     6781 tggagcagtt gcggagggca gaccgccacc gcttatatgg cagactgctc catcacacgg
     6841 taaattgaag ctttcctggt cctctccggt ctgctgggcg agggaaccat tgctggcatc
     6901 gtgttgactg catggggtgg gagcggcatg aatggagggc tcatctgcgg tggaaagatg
     6961 cagcgcctgc ccggcttgtg cgcagagagg catgatggct gccagtgctc cttgcagggg
     7021 cagcgccgcc aaggtcagtg ccattacaag cttccggaaa aatgaagaca tttggttagt
     7081 atgcgcattt tgtccacatg gagcaagagg tctttgcacg aaaagcgaga ttgtgctgta
     7141 cagtatgtat agctttatca gctttatcag ctttatcagc tttatccaag cgcttatctt
     7201 gccgagcccg gcgtccacct ctactgagcg atagcaaaac aacgttaaaa ttccagggcc
     7261 gtgctgaata tccgccataa accctttccg tacacaagac tgacgggcct cctcatccct
     7321 ctcgttgccc ttgttatgac cgcagcaatt atttgcacgg ctttgcctgc ttccgttcat
     7381 gcttccggac ctcatgttca cggtaccgca acgctcgaaa ttgcgataga tgatgcagtg
     7441 gtgcacgtaa acctgagcag cccgctggat agtttgctcg ggttcgagca tgcaccccgt
     7501 aatgagaaag agcgtcaggc tgtaaggtca atggcgtcga agctccacca ggtggataat
     7561 gttttggttt ttacgcccca ggcacagtgt cgcctggagt cagccaaact ggagtcttcc
     7621 ctgctttctt ctgaattgct gatgtcgagc tttgacaagg gcaaagcgga tggggccgac
     7681 gcggctaaac agggcacgcc gcagatcagg ccccagacaa ggttacccgt tctttcttcc
     7741 gcaactgctt cccaggccgc ttctgcagat gagcatcatg atgaacacgc cgagcttgaa
     7801 ggaacctgga gtttcaggtg tgcaacgccg ggagcattgg ccggagccga ggtacgcttg
     7861 ttccatgcat ttcctggctt gcgccggctg gatgtggcaa tagcgggtcc gaaagggcaa
     7921 agaagcgcga agctttcgcc tgcgtccagg cagctgaaat ggtagttagc gtgccgaatc
     7981 tcgcctcaca tgaatccggc atcaagcagt tcgctgtcga gatcaagaac ctggttttcc
     8041 agtggccggg caggaatggt ttcaggctat caatccccga tttcagggtg caacggggtg
     8101 aacgcgtctt tctgcaaggt gcgagcggca gcgggaaaag caccctgctc ggcttgctgg
     8161 gtggcgtgct ggtgccccaa ccatgccgcc tgcgggtgct cgacaccgac ctgacagccc
     8221 tgtcatcggg tgcccgcgac cgcttccgcg tcgatcacct gggttttctg ttccagcaat
     8281 tcaacctggt gccttacctc tccgtccgcg aaaacgtgct cctgccctgc cgcttttcgc
     8341 gcctcaggcg gcagcgggcg cttgaaacgg tcaattccag cgggacagct cctggtcagc
     8401 ctgatgctct cggccgatct gccgaccacc tgctcgcccg gtttggacta ggggcggcgt
     8461 tggcacgcca accggccaac gccctgtccg tcggacagca gcagcgcgtg gctgcggccc
     8521 gtgcactgat cgggcacccc gaactgatca tcgcggatga gccaacgtcg gcgctcgacg
     8581 ccgaccggca ggcggagttc ctcgatgtat tgctggagcg ctgcgcggat gagagcgtta
     8641 ccctgatttt cgttagccat gatgaacggc tggcggggca ttttactcga aggattcacc
     8701 tgaacgagat atctcatcac actgaataac aggagccgcg ctgccttccg gcagccaatg
     8761 agcgcattcg gccgggctcg aactggtgcg gtacttggcc cttgagcttc ttgcaaaact
     8821 attgtgaacc ccatactgct cagtgcttga gctgcccgcc ccgcccagag cacccatgcc
     8881 tgcttgcgct ggaccaccac ggttgcgtgc ccgggtacaa tgaaaacgcc ggaccgccta
     8941 cgatagcagc ggtgccgaag ttgttcacta cctgcacaga gtatagtcca ggttttccag
     9001 cgcaatcaca tgaactactc tacggtgaca ttgacgcaag cctgcgcccc taccggagca
     9061 tgcgccttat tgacgacgtt gatgcatatt ttatgcagcc caactgtgag ctttcccacc
     9121 gagtggctcc ctttcattgc atggagtact acgggttcgt caccgtccac gtaaagatgc
     9181 acatgattgc cgtttgaacc cagtgttgcc tcgtagacaa cgtcgacttc cgtcctcgga
     9241 ctgaatttgg cgccgttctg aggcgaggaa attgtgacgg aagcgtcggc ggcaagcgcg
     9301 gcggggacat ggctgcccaa aatgagcaga gccaggaatg cataaaatag gcgcaccatt
     9361 attctccttg aaaacagttg gacaaaattt catcgaaaaa agaacctcgt tcattgcaat
     9421 tggccagaat cggttagtca cctggatggt gctttaccca taatccatgg gattcctgtc
     9481 gtttacaccc acccaagctt atgcctgatg ctaccggacg gttgaactgg aaccgtgaga
     9541 atcagcacgg attccactta aaatactcaa gtcactattc aaacaagcgg accccccctc
     9601 tggatgtctg aggctttctt tttccagaaa ccgcttcagg acaaccgctt cgttatgcac
     9661 ttgatcgcgc gcaccgtaga ccaaggtaac cgttccttct ttcacctcac tgcctatcga
     9721 cttgagctga ttggggcgat gtccgaactc ggtttgatag cgttcgcgaa agcttcgcca
     9781 cttctccggg gcgtgcccga accatttccg aagctctgta ctcggcgcga tatctttcag
     9841 ccacagatcg acgtttgctt tttcttttgt caaacctcgc ggccagagcc tgtccacgag
     9901 aattccccgg tcattccctg cctctggctc ctcatagact ctctctgttt ttagctcatg
     9961 tttcgctcta gcaccgctca atagcctgaa aactcctcgg tacgaatatt gtcatcatcg
    10021 acccccgatt cattcaatgc cttacgcatc gcagctacca tcccctgagg gccggcgatg
    10081 tagaaaatag gaagcgtcag ctcgttgatg tacttcaaaa gcatcgcttt ggcgatgaat
    10141 cccacttcac cctgccattc ctggctggat tttcccatct cggtcatggt gccaataaaa
    10201 gtgtaatgag ggtttttctc gtgtggcctc ataagctcgt ccagaaaagc tgcgtcctca
    10261 ggcctgttgt tggaatcaaa gacaaaaatc tcgcgcgcca cctggtcgtg aatggtttgc
    10321 agaacgatac ttcttacggg tgtcacgccg atgccgccgc tgagaaaaac ggcgggaatt
    10381 gccgcattgt tatggagcgt gaaggatccg ccggggccct ccatcttgag ctccgtacca
    10441 agcggtatca ctttcagcac gcgcttgaag gctgtgtcac gcatccgtgt tgccaccatc
    10501 agatcgtctt cataaggagc gctcgctaaa gaaaacccgc gcgtgttgcc ttccgcgtca
    10561 gtttcaggcg gatcgatcaa ggtgaaatcc gcccactggc ctggcttata agaaaatccc
    10621 tcgggctttc cgaaacgaaa tgccattgtc cctgaggcaa cttcttcacg acctttcagg
    10681 ataaccttgt aaattgtcat cgtccctatc tccttttttg cggttcatga ttcggtcgct
    10741 cgaagcaact gcgcttcgaa actcttctga gagcccttag gcgagcttac tattccattg
    10801 ctgccccgac ctcaatatca actgaggctc gagtccttga atccgcttga gtccttgagt
    10861 ccctttgtaa cgcctataag aatatgaaac gggaaaaaat ttttgttcct gacgggtcat
    10921 gcatgaaagg gaagagggtt tgaagcaata ttttatggat gaaatcaccc agatgcgggc
    10981 atacgaaacg tgaaacaagt ttcttccgca ttcgatgaga ccgtcaactc acctttgtgg
    11041 gcgcgggcaa tttccgatgc aatgtagagc ccgagcccta aaccattctg gctggagcta
    11101 gcatcttgac gggtaaaagg ctgaaagagt ttttcgagcg cctcgggtgg gatgggtctt
    11161 ccactattag ttacggagag ctcaaatagg tcccgttcaa gaaacgcgcg gacgcggaca
    11221 ggtccttccg gcgaaccatg cgtcagagcg tttgccagca ggttcgatag aatttgcgag
    11281 aggcgggcag agtcgcaatc gacggggtca agcaggcgaa attcggcctc aatcgctctt
    11341 ttaggccatg cggtacgcaa ttcatcgaca acatggcgga gaaccggctc taaaagaaca
    11401 ggctgccggt tcagcatcat cccgcccccc atgcgtccgc gcgcgaagtc caagatgttg
    11461 tcgataagct cggccatgcg tgcggcgctt tctcgcatca tattaataag gccggtgtca
    11521 cgctcgctga gtggagagga gacaaggagc tcagctccac ccattatcgc ccccagcgga
    11581 ttgcgcagat catgtcccag gacagcgatg aattgctcgc gcagaaccga aattgcttgc
    11641 tcatctgcca gcctggtctc cgcaattgtt ctggtatagc gtaaattctc ttcgtagagg
    11701 cgccgatgcg tcgctttaaa aactgtcaca cggataaact gtggcttgct atcggcatcg
    11761 cggcgttcat acgcatttac caggacgggc aaacgttcgt caccctggca ggccagctca
    11821 agcgccactt cttcgaaaaa accctgcatc cgcaataacg gaaacagatg cgtctcgcaa
    11881 taaatttttc cgccaatcgt aagcagttcg gaaaaacgga gacccttaag gttttcggtc
    11941 gcacaaccca gccagccggc gaggcgcgaa ttgctccgca gaatttcgcc cctggaattc
    12001 attgtcaaaa agccgcagcg ggaattctcg aagaaatctt cgaaatcctc ctggagagga
    12061 tgcaaaggcg agtggtcggc gggaaccgcc atttacaaaa aggccttcat ggcagcgata
    12121 gtttcttccg gagcgctgag gttcgggcaa tgcccgctgg cttccatcaa aacaagctta
    12181 ctgttcggca tttgacggtg catgtattca ccgacaacct ggggggcgat ggcatcgttc
    12241 gagcattgta gaattaatgc gggaatagcg acgtcagcca gaatactacg gcaatcggaa
    12301 agaaaagtag tgcgagcaaa ttgcctggcg atttcggggt cggtgcggca aaaactattg
    12361 accaattcct cgcccaactt tttgcagtcg gggcaaccca tcaagatggg cgctaccgtc
    12421 atagaccatc ccatgtgatt actatcgagg gattccacga tctcttcgac ctgagcccgt
    12481 gtgaacccgc cgatatagtc accctggtta atataggacg gcgaaggcgc taccagaacg
    12541 agagctttga atatatccgg cgctttggcc gccgccataa tgcccatcat cgcgccgacg
    12601 gaatgtccca cgaacaccgc atccttcaat cggagttcac gggctatttc gataatatcc
    12661 tcggcatatc cttccagggt atcgtacttc tcccgagaat aggccgtcag gtcggaacgg
    12721 ccggctccaa cgtgatcgaa caaaatggtt tgatagctat ttgcgaaagc tggggccacg
    12781 aaacgccaca tgttctggtc gcaaccaaag ccgtgggcga acatcatagg cgtcgaacct
    12841 ttgcctcgta caatgacgtt attacgtttt agtgggctca tgataaaact cgtccggtat
    12901 tcctttccga tagggacact cgggaaagat tgataccttt atcgactaat caagatcggt
    12961 aaatcttgac gcttcaatat cttctgctga gaaacacttg tttccccagc ctggctgtca
    13021 tggagaaaca atcgtaacta aaacaactcg acttgaatcc ggtagccgga ggaacatcgg
    13081 gtttttgaaa tgaggagtgc gaggccttgg agaacatgcg ctcgttccaa gcttttaaat
    13141 ataagtccct tttttgatcc aggttgcatc aagatcggca tttccgactg cagcactgtt
    13201 gctcaattgg ggcaatgcgc ttgtcacgcc atccggtatg ctgccctctt cattaaccgc
    13261 attcggctca gcaatcgcgc tgcacttgga gcggtctgcc aattgcacca gtgttgcgtc
    13321 aatcaccgca ccataagcgt gcttgaccat aaggccgtga gcttgcaact gggcgtttat
    13381 gagtccaagc aatccgccaa gcttgtgtgt cgtaatgagg aaactgcgaa agcggtatag
    13441 caatgcctca tccggtacat cgttagccag gcttaagccg cagaaataca tgaagtcgat
    13501 ccgcaccctt agtgcttctt caagcttcag gtccgataag ctgtgccact gccccaatag
    13561 cactgccttg aacatagtca atggatcaac ggacttttgt ctttcggccc ggttggcttc
    13621 acgcttgcag agttcaagca gttcgacacg gagtccttcc cagtcaataa gcgcatggac
    13681 tttgagcaat gcgctgtcgc gtcccacgcg tgcatccatt tccatcgctc caaaactcat
    13741 ctgcatcgga ttgcgcctcc tctatcactg ttgtagaaaa cggttcctgg cagtttccag
    13801 cttcctgact tgcgtcagat tttttgcagt ctgatacggt tttttttcca tattgctcgg
    13861 cataacaccc actgaaaagg ctcttttttg agctatgttc cgggctacgc ttaacctagg
    13921 aacgcgaaag aagtgcagca ggtgaatgca cgctaccaac cgagggatac tttgtcaaca
    13981 ttattatgtc taaagtattc ggatatgtaa cagtacgtta gtacttttct tatgggtata
    14041 gacgggagaa cgcgatgctc aaaagaccgc aagagacagt accaggggca ctgcaaagcc
    14101 cgaaaaaagc tttatttaga taatcgaggt taacctacag gaggatcaga acatgtccga
    14161 ccccgccacg caagaccgtg ccgccggcgc catcatgggc gcttttatcg gggatgcctt
    14221 aggcgtcggc cctcattggt gctacgatct cgcagtgctt cgttgcgact acgggcagtg
    14281 gatcacgacc tatgccgatc ccaagccggg ttcgatacca tagcgatctc aaggctggtc
    14341 agttctcgca gacgggcatc attctccggt tcatgctgcg ttcacttatc gagcgcggca
    14401 gctatgatga ggcggacttc tgtcggagca tggatgagga aatcttcccg ttgctcaacg
    14461 gcatgccggt gaacggaccg ggatataccc aaccgtcgat tcgggataca tggcgcaagc
    14521 gtgtccagca gaaccagcca tgggggcaaa tgggaggcga ggcggatacc accgaagcga
    14581 tagggcgtac gctcgctctc gcggttcgct acgccttcca gccggaggaa ttggcagcca
    14641 ccatttccag caatacccgc ctcacccaga tcgatgatat cgcggttacc ctgactgtcg
    14701 cgtacggcgc ggtgttaagt ctcctggtcc aggcacaagc tcaacaggag cctttcgggc
    14761 aagctcatgc gattatcaag ggcgggaaaa ctgcctttcc acacgataac acataccgac
    14821 ctgcagccgc cccagacgga ctatccgcgg aaaaacgggc acttcgcctc tccgaatgcc
    14881 ctcctcacgc cctcatatgt cgcgacggcc gcgatggacc cggatatacg gatagagcct
    14941 gcatggaaag tctccgtcgt ttatgacatg agctgcgcta tttatc
//
LOCUS       DASSHJ010000103         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_783412, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000103 DASSHJ010000000
VERSION     DASSHJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783412"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VFI43_04140"
     CDS             complement(<1..1057)
                     /locus_tag="VFI43_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET7061350.1"
                     /translation="MNGKQNAETRESRTELMLWGGLECTINRVQDTYFSQLERNGHTE
                     RASDIDSFASLGIRAIRYPVLWEHVAPNGIDEADWSWPDARLPALKEHGIAPIVGLVH
                     HGSGLSDTSLIDPHFAERLAEYAGAVASRYPWVEYYTPVNEPLTTARFSGLYGFWYPH
                     GKDDDRLFVQALLNQCRAVVLSMRAIREINPIAKLVQTDDLGKIYSTPPLSELANFYN
                     QRRWLGWDLLCGKVDAEHALWDYLTGTGIDASEILWFRDNTCPPDIIGINYYITSERW
                     LDHRIERYNGWPVSKCRSHSYIDMEPARALAAPTPGIGPLLEEAWERYQLPLAVTEVH
                     IDASREDQMRWLVEIWQA"
     gene            complement(1739..2116)
                     /locus_tag="VFI43_04145"
     CDS             complement(1739..2116)
                     /locus_tag="VFI43_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061351.1"
                     /translation="MKNFTLKNTARCAVLVGTIMFGTAGGTYAAGAGDQTGQYGDMKG
                     QGGSVSGQGGSQSGSQMRSQGGSAGGQSGMSGQSSSGQKSSGQSGQMGSSSGSSSGQG
                     NSDRPSSGQSGSGGQSGGGSGGY"
     gene            complement(2249..3259)
                     /locus_tag="VFI43_04150"
     CDS             complement(2249..3259)
                     /locus_tag="VFI43_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7061352.1"
                     /translation="MKNLTFKNATRPAVPPADGDRAAHGAASGGEKRSDEGAVRSKYF
                     LILPSAPGEERRSEGQNEAQNMAPLDSGSITFVGTATVIIRYAGLTILTDPNFLHAGD
                     HVHLGYRLTSKRLTNPAIELQNLPPIDLIVLSHMHEDHFDRYVQKNLDRNIPIVTTTQ
                     AVEILEGLGFLKLYPLETWETFTVEKGDAALSITSMPGRHGPPVVAKLLPEVMGSILD
                     FANKGRPGEYRMYISGDTMVFNDINQIPKRYPGVDLALLHLGGTKLLGLVTVTMNGKD
                     GVEMMRIIVPEKAIPIHYNDYDVFKSPLSDFEKEMEQAGLKDKIIYLKHGDQYEFHLH
                     DR"
     gene            3778..4284
                     /locus_tag="VFI43_04155"
     CDS             3778..4284
                     /locus_tag="VFI43_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7061353.1"
                     /translation="MPTTQGASTHKTTTRRSTTSKAKDAIKLLTDDHNKVKKLFKEYE
                     KLAKKNESEGKEELAQQICEELKIHSQLEEEIFYPAAREAIKDDRLINEATVEHGAAK
                     DLMSELESMKPSDPMYDAVVIVLGEYVNHHIEEEQEQIFPKCQKSKMDLEEIGSELAE
                     RKEELQKD"
     gene            complement(4588..>4850)
                     /locus_tag="VFI43_04160"
     CDS             complement(4588..>4850)
                     /locus_tag="VFI43_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HET7061354.1"
                     /translation="KAIHLFFARPPEPEQRKVEVRLRLGRWLSLALEFELGADILRTA
                     IAPTWDEIGKLAAIAALRTLLNYFLEREIRDSGETAPSGPKA"
BASE COUNT         1169 a         1367 c         1243 g         1071 t
ORIGIN      
        1 ccgcctgcca gatctccacc agccagcgca tctggtcttc acggctcgca tcgatatgca
       61 cctcagtcac cgccaacggc aactggtacc gctcccacgc ttcctcaagc aacggaccaa
      121 tcccaggggt gggcgcagcc agcgcacgag ccggttccat atcaatatag ctatggctac
      181 ggcactttga tacgggccac ccgttatagc gctcgatgcg atgatccagc cagcgttcgc
      241 tggtgatgta gtaattgatg ccgatgatgt cgggggggca ggtattgtcc ctgaaccaga
      301 gaatttcgct tgcatcgatt cccgtacccg tgaggtagtc ccagagggca tgctccgcgt
      361 cgaccttgcc gcacagcaaa tcccacccca gccagcgccg ctgattgtag aaattcgcaa
      421 gctcggataa cggtggggtg gaataaatct tgcctagatc gtccgtctgg acaagcttcg
      481 cgatggggtt gatttcacgg atggcgcgca tcgaaagcac caccgcgcgg cactggttga
      541 gcagggcctg aacgaaaagg cgatcatcat ccttcccgtg cgggtaccag aaaccgtaca
      601 ggccactgaa cctcgccgtc gtcagcggct cgttcacggg tgtgtaatat tccacccagg
      661 ggtaacggct tgccactgct cctgcatatt cagcaagacg ttcggcgaaa tgaggatcga
      721 tcaggctcgt gtcggagagt ccgctgccgt ggtgcaccag gcccacgatc ggcgcaatgc
      781 catgttcctt caacgcaggc aggcgtgcat ccggccacga ccaatccgcc tcatcgatcc
      841 cattcggagc cacatgttcc cacaacaccg ggtaacggat cgcgcggatc cccagcgaag
      901 caaagctgtc gatgtcgctt gcccgctccg tatgtccatt gcgctccagt tggctgaaat
      961 acgtgtcctg cacccgattg atcgtgcatt ccagcccacc ccaaagcatc agttccgtgc
     1021 gtgattccct agtctccgcg ttctgtttcc cattcattgc aaatcgcacc tcaacagtgg
     1081 ttgttgaact tcaatatgct ttccgccgca agggcgtcaa aaatcttccg gtaccaagca
     1141 ctcgaactat cactggggta ccgagccggt cttgcgggag ggatggcaaa cctggaagct
     1201 ttatccgcaa atgcctgata ccggctatat gcacgccagc gacactacga actgctgcgg
     1261 aactgttcag gaccgcgatg attggaagac aaatcttctc gctcggctcg gcaggatggg
     1321 ggcagttctg gcgaactgat agggatatta agagcaaatc atggtcttgg atcaactaat
     1381 tagacggcaa taacgacctt atattcaagc atccggggac aaaaggtttc ctgtattagt
     1441 cggcgattca aggggagtat tcaggaacac ttcatataga taactccggt ttaaaggcgt
     1501 tgaattacct ccggccgcct gagagacgca gcgagagggc atcagcgtgc agatgaatga
     1561 gtaacgattt gcgcgagttg ctggcagggg agcggcggaa cgccgggtca ggaacagacc
     1621 gagcgttcaa ccggaagatg atgcaatggc aacgtgtcta agactgcctg gtcatgacta
     1681 aaactgcttg gcgacaggat caggtaaata aagctgcgta ggacttgttt caaggtcttt
     1741 aataaccccc cgatccccct ccggattgac caccggagcc cgactgacct gacgacggac
     1801 gatcactgtt tccctgtccg ctggaagaac ccgaggaaga acccatttgg ccggactggc
     1861 cggacgactt ttgtcctgag ctggattgcc cactcatacc cgattgtcct cccgctgaac
     1921 ccccttgaga acgcatctgg gatcccgatt gcgaaccacc ctgccccgat accgatcctc
     1981 cttgcccctt catatcgcca tactgccccg tctggtctcc tgctccggcc gcgtaggtgc
     2041 cgccggcggt gccgaacatg atcgtcccta ccaataccgc gcatcgtgcc gtatttttca
     2101 gagtaaagtt tttcatgatg ccctccatgt cattaatcat taatcagcaa aattgcaatg
     2161 acatgctaac cccatcgcag aatcgttccg tacgcttccg tacgaactct cgaaaagagc
     2221 gatgccacgc aagccggcca cccctcggtc agcggtcatg cagatggaat tcatactgat
     2281 ccccatgctt caaatagatg attttatcct tgaggcccgc ctgctccatc tccttctcaa
     2341 agtcgctgag cggagatttg aagacgtcat aatcgttgta gtgaatagga attgcctttt
     2401 ccggcacgat tatccgcatc atttcgaccc cgtctttccc attcatggtt accgtcacga
     2461 gtccgagcag cttggtgccc ccaagatgca gcagcgccag atccaccccg ggataccgct
     2521 ttggaatctg gttaatatca ttgaagacca tcgtgtctcc gcttatatac atgcggtact
     2581 cccccggccg gcccttgttg gcaaaatcaa ggatagagcc catcacttcc ggtaaaagct
     2641 tcgcaacgac agggggtccg tgacgtcctg gcatcgacgt gatgcttagc gcggcgtccc
     2701 ctttctcaac agtgaacgtt tcccacgttt ccagtggata aagcttcagg aaaccgagtc
     2761 cttcgagaat ttcgacagcc tgggtcgtcg tcactatagg aatatttcgg tcgagatttt
     2821 tctgaacata cctgtcgaaa tgatcctcgt gcatgtgaga cagcacgatc agatcgatcg
     2881 gcggcaggtt ctgcagttcg atagctggat tcgtgagccg tttcgacgtc aagcgatatc
     2941 caaggtggac atggtcccca gcatgaagaa aattcggatc cgtcaaaata gttaatcccg
     3001 cataacggat aataaccgtc gccgtcccaa caaaagtaat tgaaccggaa tcgagcggcg
     3061 ccatattttg cgcctcattt tgcccctcac tgcgtcgctc ctccccagga gcggagggca
     3121 agatcagaaa atatttgctt cgtaccgctc cttcatcgga acgtttctct ccgccgcttg
     3181 ctgctccatg cgctgcgcgg tccccatcag ctgggggtac cgctggcctc gtcgcatttt
     3241 tgaatgttaa gtttttcatt gatccctcca tgtcatcatc ggcggatgac atgcaacatg
     3301 ctaaccacat tgcaggaccg ttccgtacgg tggcatacca atcagggaaa gacggaatta
     3361 gcgaaggaaa aaggcggggc tagccaggaa aaacagcagc gtcaactgga gtcggatgat
     3421 atggaaccgg taaatggaat agttacgcct ctcctacggc cgccagctgg atgcaacatg
     3481 catttaatcc atcttttccc tcggttctga gaggggaggg agcgagtgtg ggggtgttgg
     3541 caaaccggga cgacggccga tcaacttctg ctattgtcct atgcgtaagg gataaactcc
     3601 agcccgcgtg gaaaagccgg atcacatcag accgacagta agtgtggctt accgaacaga
     3661 atcgatgctg catgcagcgt acttttgccg ctgttcgaat tgcagacaag agggatcaat
     3721 ctcgttgccg ctcgcacaca catagtcgat tcaacccatt acctcaacgg aggcggtatg
     3781 ccaacaaccc aaggcgccag cacgcacaaa accacgacac gacgcagcac gacaagcaag
     3841 gcaaaggacg cgatcaagct gctgaccgac gatcacaaca aggttaaaaa gcttttcaag
     3901 gagtatgaga aactagccaa aaagaatgag tcggaaggca aggaagaact ggctcagcaa
     3961 atctgcgaag agttgaaaat ccattcgcaa ctggaggaag aaatatttta cccggctgca
     4021 cgggaagcta ttaaagatga tcgcttgatc aacgaggcga ctgtcgagca tggagcggcg
     4081 aaagacctaa tgtctgaatt ggaatccatg aagccttccg acccgatgta cgatgcggtc
     4141 gtaatcgtac tgggcgaata tgtgaatcac cacatagagg aagaacagga gcagatattc
     4201 cccaagtgcc agaaatctaa aatggatctc gaggaaatcg gttccgagtt ggcggagcgt
     4261 aaagaggaat tgcagaaaga ttgaggttga acagacaaag gcggccgggc tgcttcatct
     4321 cccgtccgcc cttttctgga caacatcagg cgggtgaatg tttaccggcg actcggtctc
     4381 atttctgaag gttcgccgac tcgtttagtg cgttggaaat accgatcctg aaatccatag
     4441 cgcattagca aaatggggga tggaagcata gccagttctc gcctccgcgc tgccacttct
     4501 aatttgctgt ttttcgcatc atttgtttac cttccactca gcttgggctt cgctaaccgc
     4561 tttcccaacc gcttctccag catgaattca cgccttcggc cctgacggtg cagtttctcc
     4621 actatctcga atctcccgct ccaggaagta attcagcagc gttcgaagcg cagcaatcgc
     4681 cgcaagcttc ccgatctcat cccatgtagg cgcaatggct gtccgcagga tatcagcccc
     4741 gagctcaaat tcgagcgcca gagagagcca tctccccagc ctcaaacgga cctcgacttt
     4801 tcgttgctcc ggctctggag gacgtgcaaa aaacaggtgg attgccttga
//
LOCUS       DASSHJ010000104         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_276446, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000104 DASSHJ010000000
VERSION     DASSHJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276446"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFI43_04165"
     CDS             complement(<1..483)
                     /locus_tag="VFI43_04165"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HET7061355.1"
                     /translation="MNAARSTAWHPAPSLISSRARGWLQNSGSLTQLIQERCHGCFQV
                     KPVFQSLATASVDELALMKLRRKELALVREVYLYCGSTPVVFAHSVVARKDLRGAWRG
                     LSGLGNRSLGTVLFTNPVVKRTRLRFRKLTSSHPLFERACRKLQKKPSALWARRSLFS
                     L"
     gene            complement(749..3205)
                     /locus_tag="VFI43_04170"
     CDS             complement(749..3205)
                     /locus_tag="VFI43_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7061356.1"
                     /translation="MLPLSRARPPFIAIALLSACVLAYEVVLTRLFSIVLWHHFAYMI
                     ISAAMLGYGASGTALALLKEKIGPHFGPVYVSAAAGTGLLMPTAFLLAQQIPYNPLEL
                     LWDTAQLARLLGIYLLMMLPFFLGGLSIGMIWFRFGREADRIYASDILGAGTGTLGVI
                     GLLFLLPPLKLLFVLSALALLAAAIAVVELSMKKKWLPMVLLGLALAAVAVPSGNWLQ
                     LHPSPYKDLSQTLNVAGARVIGQSSSPLGQITVVENTRIPLRSAPGMSLNSTDEPPPQ
                     LGIFVDGNGPSALTRFDGRLQPLAYLDQVTSALPYHVLEHRHESPPTYPYQPHVLVLG
                     AGAGSDVLQAIYHHASRIDAVELDGNVIDLVRSNRFQDFDGNLYGLPDVYVYESEARG
                     FANSTAQHFDLIQVALLDSFGVASAGLYGLSESYLYTVEALQTYLSRLKPNGLLAVTR
                     WLSLPPRDTLKLFATAVFALQREGISNPSARLAMVRSWKTVTLLIKNSDFTRREIEAI
                     KQFSAERSFDVAYYPGMRANEANHYNVLDTPYFFEGAEALLGPQQQDFMDRYKFYIEP
                     ATDDKPYFFRFFKWSTVEELFALRRQGGMPLLDWSYPLLVATLIQALAASILLILTPL
                     AASRARRSLPSAPVAIYFLAIGMAFMFMEIAFIQKFLLFLSHPLYAVAVVLCAFLVFA
                     ALGSRLAGLRLEKADNNQRLTLAVLGIGSLSLLYLAILPGVFQTLIHLSDAARIAISV
                     ALIAPLAVCMGMPFPTGMARLAAISTPAIPWAWAFNGFASVVGAVLATLLAIHLGFAA
                     VILLAVMIYGIAAASLKKFR"
     gene            complement(3281..>3485)
                     /locus_tag="VFI43_04175"
     CDS             complement(3281..>3485)
                     /locus_tag="VFI43_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET7061357.1"
                     /translation="DGIIVTAAAGQVPPPLLKQLKPGGRMVIPLGMQFMTQYLMLVEK
                     RADGSITTRQILPVAFVPLTGSH"
BASE COUNT          851 a          953 c         1025 g          656 t
ORIGIN      
        1 caaactgaac agcgagcggc gcgcccacag ggctgaaggt tttttctgga gcttgcggca
       61 cgcgcgctca aaaagagggt ggctcgacgt cagtttcctg aagcgaagcc gcgttcgttt
      121 cacgaccggg ttggtaaata atactgttcc cagtgatcgg ttgcccagcc cgctcaatcc
      181 ccgccatgca ccccgcaagt ccttccgcgc tacgaccgaa tgggcgaaca cgaccggggt
      241 gctgccgcaa tataagtaga cttcgcgaac caacgccagt tctttccgcc gcagcttcat
      301 cagggccagt tcgtctacgc tcgcagtagc cagcgactgg aatactggtt tgacctgaaa
      361 acacccatga cagcgttcct ggataagctg cgtgagcgat ccgctgttct gcagccagcc
      421 gcgagcacgt gaagatatca aggatggtgc gggatgccat gcggtggacc tggcagcatt
      481 cattcggcga acagaaaagg gtgaattatg ccgcataacg cagagatatt gtttttgcga
      541 tcaccaagct acccgtacct gatggagtca gccaagagtt aaagcgcaac cctggaccct
      601 aattcggtgg atggaaatag tttcgtttca aagcggaccg ggcaggaaaa gcgcggcaaa
      661 aagatgggtg gcaaaaggat gaacggaggg acaacggtgg catataaaag cgggagttac
      721 tgaaagcgaa tcttcaaagg caaaggcttc agcgaaattt tttgagcgac gccgccgcta
      781 ttccatagat cattaccgcc aggagaatga ccgctgcaaa gccgaggtgg atagccagga
      841 gtgtggccag gacagcgccc acgaccgaag cgaagccatt gaaggcccaa gcccagggta
      901 tggcgggcgt ggatatcgcg gccaaccgtg ccatgccagt tggaaaaggc attcccatac
      961 atacagcaag cggagcgatc agtgccacgg aaattgcaat ccttgccgcg tcggacaaat
     1021 ggatgagtgt ctggaacacg cccggcagga tggccagata aagcaacgac aagcttccta
     1081 tccccagcac agccaacgtc aagcgctggt tattgtcggc cttttccagc cgtaggccgg
     1141 caagcctgct tcccagggcc gcaaatacca ggaaagcgca cagcacaacc gccactgcat
     1201 aaagcgggtg tgacaggaac agcaggaatt tctggatgaa ggcgatttcc atgaacatga
     1261 aagccatgcc aatagcaaga aagtaaatcg ccaccggggc ggaggggagg cttcggcggg
     1321 cacgcgatgc ggcaagcggc gtcaggatga gaagaatgct tgcggcaagg gcctgtatca
     1381 acgtcgcaac cagtaaaggg tagctccagt cgagcagggg cattccgccc tgcctgcgta
     1441 acgcgaacaa ctcctctacg gtactccatt tgaagaaacg gaagaaataa ggcttgtcgt
     1501 cggtcgctgg ctcgatataa aacttgtacc gatccatgaa atcctgctgt tgcgggccga
     1561 gcaaggcttc ggccccctcg aaaaagtagg gtgtgtccag cacgttgtag tggttggctt
     1621 catttgcgcg cattcctggg taataggcca cgtcaaacga ccgctccgca ctgaactgct
     1681 tgatcgcttc gatttcccgc ctagtgaaat cgctgttctt gatcagaagt gttactgttt
     1741 tccagctacg taccatcgcc agacgtgcgg aaggattgga aatcccctcg cgctgcaggg
     1801 caaaaaccgc agtggcaaac agcttcaaag tatcacgcgg cggcagggag agccagcggg
     1861 tgacggccag cagtccattg ggtttcaagc ggctcaggta ggtctgaagc gcctcgacgg
     1921 tatacagata gctttcactc agcccgtata gcccggcgga ggcgaccccg aacgaatcaa
     1981 gcagggcaac ctggatcagg tcaaagtgct gcgcagtgga gttggcgaag ccgcgcgcct
     2041 ctgactcgta cacatacaca tccggcaggc catacagatt cccgtcaaaa tcttggaaac
     2101 ggttgctgcg taccaggtcg atgacgttgc cgtccaattc cactgcgtcg attctgctcg
     2161 catgatgata gattgcctgc aatacgtcgc tgccagctcc cgcgccgagg accagcacat
     2221 gtggttgata tggatatgtt ggcggcgatt catgccggtg ttcaagcaca tggtagggca
     2281 atgccgaggt aacctggtcg agataggcaa gcggttggag cctcccgtcg aaacgcgtca
     2341 gggcggaggg gccattgccg tccacaaaaa tgcccaattg gggaggcggc tcgtccgtcg
     2401 aattgaggct catccctggg gcggagcgaa gggggatacg agtattttcg accaccgtga
     2461 tttgccccag cggacttgag ctttgcccga tcacccgcgc tccggcaaca ttcagcgtct
     2521 ggctcaagtc cttgtatgga gaaggatgta gttgtagcca attgcctgaa ggtactgcaa
     2581 ctgcggccaa ggcaagcccg agaaggacca ttggaagcca ttttttcttc attgacagct
     2641 cgacgaccgc aattgcggcg gccagcaatg ccaacgcgct caggacgaac agtaatttga
     2701 ggggtggcaa gagaaacaga agaccaatga cgcccaaagt gcccgtaccc gcgcccagga
     2761 tgtcggacgc atagattcga tctgcttccc gcccgaagcg gaaccaaatc atgccgatgc
     2821 tcagcccccc cagaaagaag ggcagcatca tcagcagata gatgccgagc agccgggcaa
     2881 gctgggccgt gtcccacagc aattcgagcg gattgtaggg aatctgttgg gcaagcagga
     2941 atgcggtggg catcaacaag cctgttccgg cggcagcgct gacgtacacc ggtccgaaat
     3001 gaggaccaat tttttccttc agcagggcaa gcgcggtacc gctcgcccca taccccagca
     3061 tggccgcgct gataatcatg taggcgaagt gatgccacag tacgatgctg aacaggcgtg
     3121 tcagcaccac ttcataggcc agcacgcacg cggacaataa tgcgattgcg ataaacggag
     3181 gccgcgcgcg cgagagtggc aacatgtcag aacccggcgt aagtttcagc cccgcttttt
     3241 agttcgatac ctgtaagcga aggccggacg acatctattt ttagtgagag cccgtcagag
     3301 ggacgaaggc gaccggtaaa atctggcgag tggttatgga gccatctgcc cgtttctcca
     3361 cgagcatcag gtactgagtc atgaattgca tgcccagggg gattaccatc cgcccacccg
     3421 gcttcagctg cttgagcagc gggggcggta cctgtccggc tgcagccgtc acgatgatgc
     3481 cgtcg
//
LOCUS       DASSHJ010000105        23155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1216908,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000105 DASSHJ010000000
VERSION     DASSHJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 23155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23155
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216908"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..1204
                     /locus_tag="VFI43_04180"
                     /pseudo
     CDS             519..1204
                     /locus_tag="VFI43_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611728.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1193..1840)
                     /locus_tag="VFI43_04185"
     CDS             complement(1193..1840)
                     /locus_tag="VFI43_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HET7061358.1"
                     /translation="MTALRGSVFALITAITVAGCAAIPETRFYTLSVRSEPPEVKALP
                     RDPSAPIFIDILPVNVPERLARPQLVVRFRESGTETQLFILEQDRWSSAFNYELRDAF
                     ASGIANQTGAVNGARGTRAPGQASYRIAIELTQFDAIVDDSVKARFSWTITRPVHARS
                     AACYAAIAEPVIGGVEGVVEGVQRMVAAVVADVAKTLIELDMGHVPTCAAQKKAL"
     gene            complement(1837..3495)
                     /locus_tag="VFI43_04190"
     CDS             complement(1837..3495)
                     /locus_tag="VFI43_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7061359.1"
                     /translation="MNQDEAGNSAEGLPLPDKVRQRNWVSSLIWLIPILAAVIGVSLM
                     VQTWVQRGPEITITFRSGEGLEAGKTKVKYKEVEIGTVQSIALSEDRTHVLVTVRLKK
                     EAKSFTAADSRFWVVRPRVSGLQVSGLATLLFGAYIGADAGISTELTDKFVGLETPPI
                     VTRDISGKQYLLNAADMGSLDIGSPVYFRRIKVGQVTAYDLDDNGKGVIIRIFINSPY
                     DKLVSINTRFWHASGIDMQINAEGLTLKSESLTTVLLGGLSFQSPDDNSGPPAKENTA
                     FVLAENQTEALKGPDGEAVMILLNFKQSVRGLRPGADADFRGLVLGQVKSIGIEYDPK
                     QQEFRMPVLVQIYPERLGQKYAEYRKESKYTAQQRLQFMIDRGLRAQLRTANILTGQL
                     YVAFDFFPKFAVTKRDVSRSKAEVDHGHALLELPTIPRSGEEIQTQISEIALKLSKVP
                     FDQIGSELQESLKILQGTLNSAGQLAEKLNNDVAPEMAMAMRDVRDMLDAAERTLSDD
                     APLQRDLRQTLQELTRAAVSLRVLTDYLERHPEAVIRGRRGDKQ"
     gene            complement(3473..4198)
                     /locus_tag="VFI43_04195"
     CDS             complement(3473..4198)
                     /locus_tag="VFI43_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HET7061360.1"
                     /translation="MPANRTVRTVQPASQPPESGLPISGFSVQTAAKLGMASCPHCAT
                     LWEDVKAHDRCGRCGSGLSIRKPDSFNRTTAFLIAAYIMYVPANTLPVMITIPFLGKE
                     QQDTIMSGVIYFWNSGSWVLALIVFIASFVVPLFKLAVITLLLIAIQRRSRQGLLLRA
                     KLYRFVAVIGRWSMLDVFVVTLMTGLVQIKGFAVVHPGAGIAAFAAVVVLTMLASISF
                     DPRLMWDVTEHEPGNPHESRRGG"
     gene            complement(4185..4823)
                     /locus_tag="VFI43_04200"
     CDS             complement(4185..4823)
                     /locus_tag="VFI43_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HET7061361.1"
                     /translation="MSGLIACEECDAVYRKLALGPSEVALCGRCGAELERDRHALRRH
                     VLPLSIAGLCMYIIANVFPILQLEVRGDFSQATLIGSVHSLNAEGMPLVAFLVLATTI
                     LFPLFQLLALIYLLALPIRLRRSGYPAAFNLSGRLIQILRPWVMVEIFLLGVIVAFVK
                     LTSMATVLPGIALWAFGALAFLLTSVFSFDPRHIYIRQISLQEKKGKKHAGK"
     gene            5209..5400
                     /locus_tag="VFI43_04205"
     CDS             5209..5400
                     /locus_tag="VFI43_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061362.1"
                     /translation="MLGAESLAADHEQSAYLESGREPLPLETSIPGVFAIGDARASST
                     KSDFPSSYGRFITAVRKLG"
     gene            5706..9041
                     /locus_tag="VFI43_04210"
     CDS             5706..9041
                     /locus_tag="VFI43_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7061363.1"
                     /translation="MTIQKLSFIFYLVTAILISLLALFIGLRFFEQRELEHFREMRYA
                     SFLAVDELRQSSDDLTRMARAYVDTGNPQFERMYWEILAIRNGEKERPLHYERSYWDL
                     VTGDPGLQPSPAGQKISLRKRLEELGFTPQEFAKLQKAEDNSNQLVQLEHRAFNSMKG
                     LFQGRFGEFDVKGTPNPELARSLLNGENYYASKARIMRAVNEFHELFDKRTLDSVAIA
                     QQRTNLYFSGTILIFVLLLAWLILSSRVVWRKVQNLMQLEEETRNIGKSRNVSQFEIS
                     SNDEIGNLSRAFIAAQAERDRYFDQSLNLLAIAKFDGQFKRLNPAWSREFGFSPEELS
                     SRSFIDFIAPRSRTKAEAELDKLVACRGDSFEIQMACKDGSLRWVLWNITALAEAREF
                     YISGQDITERKNTEIDLQNAQKAAEAASRAKSEFLANMSHEIRTPMNGVLGTVDLLLN
                     TSLTKSQRELARLARTSGETLLTIINDILDFSKIEAGKLTIAPTPFDLLETVEGVAAM
                     IAMQHTRKHDVNIIVRYPPHVPRHVIGDMGRIRQILTNLASNAAKFTDKGHVLISVEN
                     DAVDGDEATLRFSVEDSGIGIAANKLESLFDKFTQADASTTRRHGGTGLGLAISKQLV
                     KLMGGAITAKSRIGMGSTFWFTLRLPLQNNRPAEILPYTELAGVRMLIVDDNAVNRLV
                     LQEQIRGWKMRIGSCDSGAEALKTLHQAQAGGDPYRVAILDYQMPEMDGEMLGQAIKR
                     DPLLRNIELIMLSSLGQEGEVQERLKKIGFAAYLVKPARQSELLATLKNVWHAHCERR
                     AIDLISDSQALPEAQGAPVTDNPEPAFIGTRVLLAEDNVTNQIIAEMMLRSLGCDVDI
                     APDGSQAIQRIDEFSYDIVFMDCEMPEMDGFEATAEIRRRRDSKSRLPIVAVTAQAMQ
                     GDKERCLLAGMNDYVSKPVKQKDFASALKKWLLKKDGHETGKEEKTQNEDGVSCDSSH
                     VLSSLGASSPIQASPALNSEVVASLRALAEATEPALFNQMFASFLSDSGERISILRSA
                     ASTIDAPRLQKTAHAMKGASANVGAMHMAEIARQLEVLGKTATVTGAIALIEAMEREF
                     ENAKNEIDAITRPIDALQGEAQP"
     gene            9038..9916
                     /locus_tag="VFI43_04215"
     CDS             9038..9916
                     /locus_tag="VFI43_04215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7061364.1"
                     /translation="MKILIAEDDTTTRLIFGATLRKMGHAVTAVEDGQKAWKAWQQEE
                     YALLITDWIMPDLDGLELCKRIRAQPSLQYTYIILLTSMEGKGSYLEGMDAGADDFIT
                     KPFDEEQLAARLRVAERIIALHQKLHLQATHDHLTGVWNRAAIMDYLFGEVERAKRQS
                     TYIGVVIVDLDHFKRINDTYGHPAGDTVLKEAARRMQSALRPYDRLGRYGGEEFLITA
                     PDCDLSQTMALAERVRNFMSSDPVKSHSDEIPVTVSLGVAVGSDQFGEDGAALIATAD
                     EALYRAKRAGRNRVES"
     gene            10283..10612
                     /locus_tag="VFI43_04220"
     CDS             10283..10612
                     /locus_tag="VFI43_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061365.1"
                     /translation="MKNAKTVLVAIGMAAMSSIAPQAFAQEDGLVGVDIRNIKKDIAE
                     SINVDPGKIPSAVRVKANIATETCKVPEDILAQQETKGGANCVAKMTSPALNRLVDRE
                     IRGITKP"
     gene            complement(10725..13238)
                     /locus_tag="VFI43_04225"
     CDS             complement(10725..13238)
                     /locus_tag="VFI43_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7061366.1"
                     /translation="MRAGHAKVIFYSFFLVSCASFSRAADERDLRVKEPVRQESSAPI
                     KLTRHDAPEEKTDLAGSEVKMPEITVGGKVTPPAKQTDKERYRLPQTTETIMKEKIDA
                     TINLANPEDALKYLPSIQVRTRYIGDTNAPVGMRTSGTSASARNLIYADGILLSSLLG
                     NNNQNTGSPRWNTVSPSEIAQVNVLYGPFAAAYPGNSIGGVINIITKMPEKFEAGIDV
                     QSSWQTFNLYGTKDTYESQRYMGYFGHKYKDLSFRFDYSHLDAHSQPITFATALNSGT
                     IAGGDDRLVTGAFDTLNPTNAPTQVLGAGNINHTVQDNFKWKLAYDIAPAIKLAYTFG
                     MWQNSATARVQSYLRDAAGNMVQSGFVNIDGRRYNLSAANSPTFAGNLTNQTTWSHGI
                     NLRSNTGGVFDWELTGSVVDLARDTVRAPTVDPTLAGAINAPGRITSLTGSGWHTVDA
                     KGIWRPRTDLLGYHEISFGFHHDLYTLKNPVYNTGNWQTGGGQGIFSSSVGKTQTQGY
                     WIQDAWDFHQDWNFTFGGRVENWDAYDGVNITTVGAGLKTVNQADRSSVNFSPKGKLT
                     WTPMERVRIGAAIGQAYRYPTASELFQTTTVGTGATAISFNANPNLKREEALSSELSG
                     EYFRDKGRLRLSLFQERVKNAIFSQTGVVANPSTNTTSQATFISNVGEIDTYGIEFSG
                     EQSDFLVKGLDVTGNFTWTDSRIVKNEAADAAAAALGPTATNPNAASPSTGKRQPRVP
                     VWRANAVVTYRATERLTGTASVRYSSGQFNQLNNSDTNGFAYTGITSYVVVDLVAKYQ
                     LAKQVSLIGGINNVNNDKYWIFHPFPQRTYFAQIKFSYY"
     gene            complement(14684..14759)
                     /locus_tag="VFI43_04230"
     tRNA            complement(14684..14759)
                     /locus_tag="VFI43_04230"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14724..14726),aa:Met,seq:cat)
     gene            14974..16143
                     /locus_tag="VFI43_04235"
     CDS             14974..16143
                     /locus_tag="VFI43_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HET7061367.1"
                     /translation="MKFDVVVVGGGLVGVGLALALAQGSVKVALIESREPPPLPEDDS
                     WDSRVYAISPGSAEFLQNLGVWQALDQARITPVYDMAVFGDDNASRIDFSAYDIGCSA
                     LAFIVENRQLQAAVWKRLKRERKNVTLLCPAQCALMTRTESQVHLHLSDGTELQAALI
                     AGADGLNSWVREQAGIETKRQPYDQTGVVANFKAELHHRNIAHQWFRRDGVLALLPLP
                     DTMVSMVWSANEEQARKLLESSREDLCNEVVEASCGVLGDMQTVTPPAAFPLNFVHVT
                     KLVQPRLALIGDAAHGIHPLAGQGMNLGLRDARELAAIILTRGLQADCGDYLLLRRYE
                     RARKEDILALELATDGLQKLFTATHPTLIRLRNLGLEMTNRLPLLKDRLMRHALG"
     gene            16217..16927
                     /locus_tag="VFI43_04240"
     CDS             16217..16927
                     /locus_tag="VFI43_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HET7061368.1"
                     /translation="MRSKLVLPFLLACLAFGEAFADEASLKKALQGIFPDEKIESLKK
                     TPYLGLYEVVIGGELFYTDDKASYLFFGHVVDPKTRQSLTSERLQQIKEARRVNQDSL
                     PLDLAIKAVKGDGKRKLVVFSDPNCPYCKRLEKELTNITDVTIYTLLYPVLNGSIPTA
                     TAIWCSSDRLKAWDDFMLRGTAPTGKNCEAPIEKLLEAGRKNGISGTPTLIFADGSVV
                     PGMIPTDSIEKKLSSARK"
     gene            complement(16993..18186)
                     /locus_tag="VFI43_04245"
     CDS             complement(16993..18186)
                     /locus_tag="VFI43_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HET7061369.1"
                     /translation="MKNQLSFTAIAALLLLDACASRPPTAPAAPEKPAAAIPSSIPTP
                     PPVVLQPAPWNAVVGWVEDDIQPAWDAFLRSCSVLKNQPLWQETCTQASYFQGRSSDD
                     LRQFFESRFVPHEVLNPDGSGEGLITGYYEPLLKGSRQPSRRYRHPIYTTPDELLVID
                     LGALYPELKNMRLRGRLQGRKVVPFYSRAEITSNPQSLKGKELLWVDDEVDLFFLQIQ
                     GSGRVQLEDGEIVRVGYSDQNGHPYKSVGKVLVERGELELEKASMQGIREWGQKNPHK
                     LSELLQQNSSFVFFRELPANIPGPLGSLGVPLTAGRSLAIDPRAIPQGAPVFLATTWP
                     NTTQPLSRLMVAQDTGGAIKGNVRADFFWGFGAEAASQAGKMRQTGKMWVLMPLGYTT
                     NTGTR"
     gene            18607..19950
                     /gene="gdhA"
                     /locus_tag="VFI43_04250"
     CDS             18607..19950
                     /gene="gdhA"
                     /locus_tag="VFI43_04250"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET7061370.1"
                     /translation="MKYKSFQEFCQSIAERNPGQSEYMQAVAEVMESLWPFITDHPRY
                     AEHGILDRLIEPERVIIFRVAWVDDHGEVNVNRGYRIQHSSSIGPYKGGTRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGADFDPKGRSQGEVMGFCQAYMTELFRHIGSD
                     TDVPAGDIGVGGREVGYMAGMMKKLSNRSDCVFTGKGLSFGGSLIRPEATGYGTVYFA
                     EEMLKQSGRSLAGLRVSVSGSGNVAQYAVEKAMLLGAKVVTVSDSGGTVVDEEGFTSE
                     KLAELMDVKNHLRGRISEYAERVGADFLPGVRPWHVPVDVALPCATQNELDDADAATL
                     IQNGVICVAEGANMPSTAEAVKRFEHQGVLFAPGKASNAGGVATSGLEMSQNAMRLSW
                     PREEVDARLLEIMQAIHQSCLRYGRRKDGSVSYVDGANVAGFVKVADAMIGQGVI"
     gene            complement(20310..21986)
                     /locus_tag="VFI43_04255"
     CDS             complement(20310..21986)
                     /locus_tag="VFI43_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7061371.1"
                     /translation="MPPTLAPRREQMFPKLAPAEITRLRRFGTVRTWQAGELLFEPNR
                     PGTGMFVLLHGHILTTRKNGCGIEIPIIESGPGNFTGEVGQLSGRPPLAYGRALEMVE
                     ALMIDPASLRAVVVAEAEVGERIMRALILRRMALVETGGGGPLLIGPPGAADMVRLQG
                     FLSRNSHPHLVLDPALDSVAEDVMKLHQPRPNELPLVACPDGTVLKCPTERELARRLG
                     LYIDLSADRVYDAAIVGAGPAGLAAAVYAASEGLSVLVLDTYAFGGQAGASARIENYL
                     GFPTGISGQALAGRAYVQAQKFGAEMVVPVEVTSLDCSRFPLVLKLDCGVKVSSKTVV
                     IATGARYRRPGIPELDKYEGRGVYYWASPIEAALCREEEVVLVGGGNSAGQAIVFLAS
                     HAAHVHHLIRGANLAKSMSKYLIDPIGSLANVTLHTESEIVAIEGCDEGVSAVHWRNR
                     QNDSMQTMPTRRVFLFVGADPNTGWLENCGVKLNGKGFVCTGLDLSPADREQWAHSEN
                     SRVQPLQLETSAPGVFAIGDARANSIKRVAAAVGEGAAVVAQLHALLALH"
     gene            complement(22147..>23155)
                     /gene="nhaA"
                     /locus_tag="VFI43_04260"
     CDS             complement(22147..>23155)
                     /gene="nhaA"
                     /locus_tag="VFI43_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET7061372.1"
                     /translation="PVAAAIGGMVAPAILYLLLAPAGPWSHGWGVPMATDTAFAVALI
                     VMMGNRVPAELRIFLTAAAIVDDIGAIIVVAIFYSGTMSFGYLAGAAAIVAALALLNR
                     SHIYNVTPYVLLGILLWACVLASGLHATMAGVILALFIPTRPPPNLTALMVQADAILS
                     TEARRRSEVLRHGPSLPAMRALDAIHDRLESPADRLLRHAGGPSSYLVLPLFALANAG
                     VAVTGEALIGHDPLMLAVIVGLVIGKPLGLVLASVIAVRFGIATKPDEYSWRQLIGAG
                     ALAGIGFTMSLFIASQAFPTATDFAAAKIAVFAASIASAILGVAILWNADKQRGQNPG
                     P"
BASE COUNT         5502 a         6244 c         6269 g         5140 t
ORIGIN      
        1 ggggttcgat tccccctccc ggcaccaatt tcagaatgta aactccttag tttaactagg
       61 ggcctgttaa cacttaaatt gcttccccat aatccatcgt gaggcgcatc gaatttaagc
      121 tcggctctta gcgcctccat gataataagg tgaaacgctt gcgtccgtgg ctgagttgtt
      181 atccacaatt tcaaacctcg caggggagtt aaggtagaag caattccacc gtgctccaat
      241 gcggtaagcg atcataaatt ttgctttgca acgtgcctgg attcgccgaa atgtaaggta
      301 aggcaattgc catcacattg ggtcttctct tgcaaagtct ttcattacct gcgtgacccg
      361 gttcttttct tcttgggtga cgaatttgcc caccatacta taggcaattc tttgtagctc
      421 ctgccccgcc tggtcataat caccctgctg acaagcctgc tctatttcgt tgcaatgcct
      481 gatccacgct gaattagccg cactttgttc attctcgcga ggaagccata cgtagtgatg
      541 acccagtagt gtccgcggcc gaggtacggg aactcaagaa gcgcattcgc gagttggagc
      601 gcgtgctggg caagaaaacc ccggagaacg agattctcta ctgaagcggt gaagcttgcc
      661 catgaaaaaa ctgatctcgc gcatgccctt gctgccacag gatgattcgc tatgaagcca
      721 ttgcagacac gatggtgttg cccgttccca tctattgaac ggatgaagcc gaaaacacaa
      781 gccggctgca gtcgctacaa caaggccgac gatgcctgac tgttgcctct gattcgcgag
      841 cttgtggatt gtcgcatgac ttacgaatac agaaggattt gtccctgctt aaccgcaagc
      901 tcggtgagat gggcaagtct gctgacaacc acaaaagggt ctatcgcatc acgtgacaga
      961 atggactgct gctcgcccgg catacgggca agcagcgcat gctgtctcat gaaggaaaag
     1021 tcggcacctt gcgctccagt tgcgctggtg ctccaatggt tttgagattt cctgctggaa
     1081 tggccaggta gtgcgcgttg cctttgccct ggattgctgt gaccttgaaa tcatcagcca
     1141 tgtggctacc gccgacggta tccgtgagat gatgcgggat ccgatggtta tatcaaagcg
     1201 cttttttctg cgccgcgcac gttggaacat gacccatatc cagctcgatc agggtctttg
     1261 ccacatccgc gactacggcg gcaaccatgc gttgcacgcc ctctacaaca ccttcgacgc
     1321 ctcctataac gggctccgca atggctgcgt aacatgcggc gctgcgggca tggacaggac
     1381 gtgtaatcgt ccagctaaat cttgccttta cactgtcgtc gactatcgca tcgaattggg
     1441 taagctcaat tgcaattcta tagctggctt gaccaggtgc acgggttccg cgtgcgccgt
     1501 ttacagctcc ggtttggtta gcaataccag aggcgaacgc gtcccgcaat tcatagttaa
     1561 aggccgatga ccaacgatcc tgttcaagaa tgaaaagctg tgtttcggta ccagattctc
     1621 tgaaacgtac aaccagttgc gggcgggcaa gccgttctgg aacattgacc ggcaatatgt
     1681 caatgaaaat cggggcagac ggatctcgtg gcagggcctt tacctcggga ggctcggacc
     1741 gaaccgacag cgtataaaac cgcgtctccg gaatcgctgc acacccagcc accgttatgg
     1801 ctgttatcag ggcaaatacg ctgcccctta gggccgtcat tgtttatctc cccgcctgcc
     1861 tcgaattacc gcttcaggat gacgctccag ataatccgtc aatactcgaa gggaaaccgc
     1921 tgcccgcgtt aattcctgca gcgtctgacg taaatcccgc tggagcggag catcatcaga
     1981 tagggtacgc tcagcggcat ccagcatgtc acgcacgtct ctcattgcca ttgccatttc
     2041 cggcgccaca tcattattca atttctccgc cagctgtccc gcgctgttca gcgtgccctg
     2101 gaggattttt aacgactcct gtaactcgct gccgatttga tcgaagggta ccttgctcaa
     2161 cttgagcgcg atctcggaaa tctgcgtctg aatttcctct ccacttcttg gaatagtggg
     2221 caattccaac agcgcatggc catggtccac ctccgcttta cttctcgaaa catctctttt
     2281 tgttaccgcg aatttaggaa agaaatcgaa ggcaacataa agttgaccgg taaggatatt
     2341 ggcggttcgt aattgtgcgc gcaagccgcg atcgatcata aactgtaaac gctgttgcgc
     2401 tgtgtacttc gattcttttc gatactccgc atatttttgg cccaagcgct ccggataaat
     2461 ttgaaccagt acgggcatcc tgaattcttg ctgcttcgga tcgtactcaa taccgatcga
     2521 cttaacctgt cccaacacca gccccctgaa atcagcatca gcaccaggtc tgagaccccg
     2581 caccgattgc ttgaaattca gcaatatcat taccgcttcc ccatccggcc cctttaacgc
     2641 ttcagtctgg ttttccgcca gcacaaaggc agtattctcc ttggcaggag ggccggaatt
     2701 gtcatccgga gattggaacg acaagccgcc aagcagaacg gtggtcagcg attccgactt
     2761 aagcgtcagg ccttccgcat taatttgcat atcgatgccg ctcgcatgcc agaagcgtgt
     2821 attgatactc acaagcttgt catagggcga attaatgaat atccggatga tgacgccttt
     2881 gccattgtca tccagatcat acgcggttac ttgccccact ttgatccgcc ggaaatagac
     2941 aggcgaacct atatccagcg aacccatatc ggcggcgttt aaaagatact gcttcccgga
     3001 tatgtcgcgc gtgacgatgg gcggtgtctc aagtcccaca aatttgtctg tcagctccgt
     3061 tgaaatgccg gcatcggccc cgatgtaagc gccaaatagc aaggttgcaa gtcctgacac
     3121 ctgcaaacct gaaacacgtg gacgaacgac ccaaaaacgg ctgtcggctg cggtaaaact
     3181 tttggcttcc ttcttcaatc gcacggttac cagaacgtga gtgcgatctt cgctgagcgc
     3241 aattgactgg actgtgccga tctccacttc tttatacttg accttggttt tgccagcctc
     3301 gagtccttcg ccagatcgaa atgtgatggt gatctcaggg ccgcgctgaa cccaggtctg
     3361 aaccataagc gaaactccga taaccgcggc aagtatcgga atcagccaga ttaacgagga
     3421 gacccagttt cgctggcgca ccttgtcagg taaggggagg ccttcggcag aattacccgc
     3481 ctcgtcttga ttcatgtggg tttccgggct catgttcagt cacgtcccac atcagacggg
     3541 gatcaaaact gatcgaagca agcatggtga gtaccacaac ggctgcaaag gcggcgatac
     3601 ctgctccagg gtgcaccacg gcaaagcctt taatctgtac gagtccggtc atcagcgtta
     3661 ctacaaaaac atccagcatc gaccagcggc caattactgc gacaaagcga taaagttttg
     3721 ctcgcagcaa gaggccttgc ctgctgcgcc gctgtattgc gattaatagc aaggtaatga
     3781 ccgccagctt gaatagaggc accacaaagc tggcaataaa aacaataagc gccaataccc
     3841 atgaaccgga attccagaaa taaatgacgc cgctcatgat ggtgtcttgc tgctccttgc
     3901 cgaggaaagg gatggttatc atgaccggca aggtattcgc aggaacatac atgatgtagg
     3961 cagcgataag gaaagccgtt gtccggttga aactgtcggg tttccgtatg gacaatcctg
     4021 aaccgcagcg cccacaacga tcatgagctt ttacatcttc ccacaaggtg gcgcagtgag
     4081 gacatgacgc catgcccagc ttcgccgcag tctgtacaga gaagccgctt ataggcaatc
     4141 cgctctctgg tggctgactg gctggttgaa cagttctaac agttctattt gccggcatgc
     4201 ttttttccct ttttttcctg taaggatatc tgcctgatat agatatggcg cgggtcaaac
     4261 gaaaacactg aggtcagcag gaaagcaaga gcaccaaatg cccatagcgc gatcccaggc
     4321 agtacggttg ccatactcgt cagtttgaca aacgcaacga tgacgccgag tagaaatatc
     4381 tccaccatta cccacggtcg caagatttgg atcaagcgcc ccgataaatt gaacgcggct
     4441 ggatatccgg atcttctcag tctgattggt aaggcaagca aataaatcaa agccaaaagt
     4501 tgaaacaagg gaaacagaat tgttgtagcc aacaccaaaa atgctaccaa gggcattccc
     4561 tcagcattca aggaatgtac cgagcctatc agcgtcgcct ggctaaaatc accccgtacc
     4621 tccaattgaa ggatcggaaa gacattggca atgatgtaca tacacaagcc ggcaatcgac
     4681 agtggtaaaa catgcctgcg gagagcatgc ctgtcgcgtt ccaattcggc accacaacga
     4741 ccgcacagcg cgacttcgct gggacccagc gctaatttgc ggtaaaccgc atcacattct
     4801 tcgcaagcga tcaagcccga cacttcccgc atggttaatt tattgaagat tgtctttcaa
     4861 gatcctacga tttggactcc aactccgcat tccggaaatg atgactcatc cgagttcgca
     4921 taaccctacc tatatactgt agtaaatatg gatgcttgca gcggtgccag cagggaatcg
     4981 aacgggccgt gtgctgaacg gttaaatcca atcgcgggtc tgtagttacg gagttttgat
     5041 cgacacccac atggagtttt gatatgcatc atcccgttgt cctcatcact ggcgcgctca
     5101 cgggcactgg ccgtgccacc gccgttgcct tcgccaagga ggtgcacgcc tggtcgtgag
     5161 cggtcgtcgc gaagaggctg gacatgcgtt ggccgcccgg aattgcgcgt gcttggcgct
     5221 gaatctctcg ctgccgacca tgagcagtcg gcgtatctgg aaagcggtcg tgaacctcta
     5281 ccgctcgaaa cttcgattcc aggggttttc gccattggcg atgcacgtgc cagctcaaca
     5341 aaatcggact ttccttcttc gtatggccgc ttcataacgg ctgtaaggaa attggggtga
     5401 aataatgcct caaaccaaca tccacgcggc atcatcaacc ctccataata tcgctcatca
     5461 gcagtgaaac taactttgcg aggttcagcc atatcgacgg caagcatatc ctccaagttg
     5521 tttcaccatg tcctgcctga gtgggcaaga ttcagcgaga tcagccactg tagaccgtca
     5581 atcgtactgt tataaccgaa tgatcgatcg atccgagata gcggtacagt taaaaaccca
     5641 gtccaggact agcgaccgga aaagcgagct gtcctatgcg acgtggttct cccgaaggag
     5701 caatcatgac catccagaaa ttaagtttta tcttctatct cgtgacagcg atattgattt
     5761 ctctgctcgc ccttttcatc ggcctccgtt tcttcgaaca gcgggagctg gaacatttcc
     5821 gggagatgcg ctatgcatcg tttctggcgg ttgacgaact gcgtcagtca tccgatgatc
     5881 tgacgcggat ggcacgggcc tatgtcgata cgggcaatcc ccagtttgag cgaatgtatt
     5941 gggagatact tgcgatccgc aacggggaga aggaacggcc cctgcactat gaacgcagtt
     6001 actgggatct tgttaccggt gatcctggct tgcaaccctc cccagcaggc cagaagattt
     6061 ctttacgaaa gcggctggag gagctcggtt tcacccctca agaattcgca aaacttcaga
     6121 aagctgagga caattccaac cagctggtgc aattggaaca cagggcgttc aactcgatga
     6181 aaggactgtt tcaagggcgg ttcggtgagt tcgacgtaaa aggtacccct aaccccgagt
     6241 tggcccgcag tctcttgaac ggcgagaact actacgcctc gaaggccagg atcatgcgtg
     6301 cggtcaatga gttccatgaa ttgttcgaca agcgcacgct cgattcagtc gctattgccc
     6361 aacagcgcac aaatctttat ttctctggca ccatcctgat tttcgtcctc cttctggctt
     6421 ggctcattct ttcgtcccgc gtcgtatggc gaaaagtaca aaatctgatg caactcgaag
     6481 aagagacgag aaacatcgga aaatcccgca atgtctccca attcgagatt agctccaatg
     6541 acgagatagg gaatctttcc cgtgccttta ttgctgctca ggcggagcgt gaccgctatt
     6601 tcgaccagtc tctcaacctt cttgccatcg caaagtttga tggccagttc aagcgcctga
     6661 acccggcgtg gtcaagggaa ttcgggttct cgccggaaga gttatcctct cgatccttca
     6721 ttgacttcat cgctccacgt tcccgaacaa aggctgaagc agaactggat aaactggtgg
     6781 catgccgcgg agattcattc gaaatccaga tggcgtgcaa ggatggatcg ttacgctggg
     6841 tcttgtggaa cataactgcc ctggcggagg cccgggaatt ctatatctcg ggccaggaca
     6901 ttacagagag gaagaatacg gaaatcgatc tgcaaaatgc gcaaaaggca gcggaagctg
     6961 ccagccgcgc gaagagcgag tttctggcga atatgagcca cgagatccgc acacccatga
     7021 atggcgtgct gggcacggtc gacctgcttc taaatacctc gttaaccaag tcgcagcgcg
     7081 agcttgcgag gctggcacgc acaagcggcg agacactgct taccataatt aatgacattc
     7141 tcgatttctc gaaaatcgag gcgggtaaac tgaccattgc tccgaccccc tttgatcttc
     7201 tagaaacggt tgaaggggtg gcggccatga tcgccatgca gcacacccgg aagcatgatg
     7261 tcaatatcat agtgcgctat cccccgcacg tcccgcgcca cgtaatcggg gatatggggc
     7321 gcatccgtca gatattgacg aatttggcca gcaatgccgc caaattcacg gataagggtc
     7381 acgtgctcat cagtgtcgaa aacgacgcgg ttgatggcga cgaagccacg ttgcgtttca
     7441 gcgtcgagga cagcggcata ggcatcgctg caaataaact ggagagcctc ttcgataagt
     7501 tcacgcaggc agatgcttcc accacgcggc gccacggtgg aacaggcctt ggattggcca
     7561 tcagcaagca gctggtaaaa ttgatggggg gagccattac cgccaagagc cgcattggca
     7621 tgggctcaac gttctggttt accctccggc tcccgctgca aaacaatcga ccggcagaga
     7681 tactgcctta taccgaattg gccggtgttc gtatgctgat tgtggacgac aacgcggtca
     7741 accgcctcgt cctgcaggag cagatccgcg gctggaaaat gcgtatcgga agttgcgatt
     7801 ccggtgcaga agcccttaag acactacacc aagctcaggc cggaggtgat ccctaccggg
     7861 tcgccatcct ggattatcag atgccggaga tggacggaga aatgctgggt caagcgataa
     7921 aacgcgatcc ccttttgcgt aatatcgaac tgatcatgct tagctcgctg ggacaggaag
     7981 gagaagtcca ggagcgtctc aagaagattg gttttgccgc ctatctagtc aaaccggcgc
     8041 gccaatcgga actattggca acgctcaaaa atgtttggca tgcccattgc gagcgacgcg
     8101 caatagacct gatcagtgat tcgcaagccc tcccggaagc tcaaggggca ccggtaactg
     8161 acaacccgga acccgcgttt atcggcacgc gtgtgctgct ggctgaggat aacgtaacta
     8221 accagatcat cgcagagatg atgctgcgca gcctgggctg cgatgtcgac attgcgcccg
     8281 atggcagtca agcaatacag agaatagatg aattctccta cgatattgta ttcatggatt
     8341 gcgaaatgcc agaaatggat ggatttgaag ccactgctga gatccgccgt agacgcgaca
     8401 gcaaatcccg gctccccatc gtcgccgtga cggctcaggc aatgcaaggc gacaaggagc
     8461 gttgtctgct tgcaggtatg aatgattacg taagcaaacc ggtaaagcag aaagattttg
     8521 cgtctgcctt gaaaaagtgg ctgctgaaga aagatggaca tgaaacaggc aaggaagaga
     8581 aaacacaaaa tgaggacgga gtcagctgcg attcctcgca tgtcttatcc tcccttggcg
     8641 cttcgtcccc tatacaggcc tcgccggcat tgaacagcga ggtcgtggca agcttgcgtg
     8701 cattggcgga agcaacggaa ccggccttgt tcaaccagat gtttgcatcg ttcctgagcg
     8761 acagcgggga acgtatcagt atcctgagaa gcgcagcaag cacgatcgat gccccgcgcc
     8821 tgcaaaaaac cgctcatgcg atgaaaggcg ccagtgcgaa cgttggggca atgcacatgg
     8881 cagagattgc ccggcaactg gaagttttag gcaagaccgc tacggtgacg ggagccatcg
     8941 cgctgataga agcaatggaa agagagtttg agaacgcgaa gaacgagatt gatgcgatca
     9001 cgagaccgat tgatgcgttg cagggggaag ctcagccatg aaaatcttga ttgcggaaga
     9061 cgataccacc acccgcctga ttttcggtgc tactttaagg aaaatggggc acgccgtgac
     9121 ggcagtagaa gacgggcaga aagcatggaa ggcctggcag caggaagaat acgcgctgct
     9181 catcactgac tggataatgc cggacctcga cggcctggaa ttatgcaaga ggatccgggc
     9241 ccagcccagc ttgcaatata cctacattat ccttcttacc tcaatggagg gtaaaggtag
     9301 ttatctcgaa ggcatggacg cgggggccga tgattttatt accaagccct tcgatgagga
     9361 acaactggca gcgcggctac gggtagccga acgaataatt gccttgcatc agaaactgca
     9421 tctccaggcc acgcacgatc atcttaccgg tgtttggaac cgggccgcca tcatggacta
     9481 tctgtttgga gaggtggaac gagcaaaacg gcagagtacc tatatagggg tggtaattgt
     9541 cgacctcgac cacttcaagc gaatcaacga cacctacgga caccccgctg gcgataccgt
     9601 cttgaaggaa gctgcccgac gcatgcagtc cgcccttcgt ccctatgacc ggctggggcg
     9661 ctatggtggc gaggagtttc tcattactgc ccccgattgc gatttgagcc agacgatggc
     9721 ccttgctgaa cgggtccgaa actttatgag ctcagatcct gttaaaagtc actctgatga
     9781 aattcctgtg actgtgagcc tgggagtagc ggttggtagt gatcagtttg gcgaagatgg
     9841 agctgccctg atcgccactg ctgatgaggc gctctaccgg gcaaaaagag caggccgtaa
     9901 ccgggtcgaa tcctgacaaa aacatgcaag actaagactg tgagctcgaa gccaggtgcg
     9961 ttatcttcct gacaggggcc gctcgcttca aatcacccgg gagatacaga ataatgaata
    10021 tctggttccc ttttttaagg tgccccaaat ccttctgctt attgtcaatc tccagccagg
    10081 acctgacgtt cccccaacgc ggcgttcaca cgatcggttc attcgtgtag ttctaatcat
    10141 atctcttcac cggatgcata tctttgttgc ccagcgcacc gagaacgctt tccgtcacag
    10201 ggtataaaac ataacttcta agacatccct gggcgcaacg taaagagcgc gctcccctat
    10261 ccccattgat caggagatga aaatgaaaaa tgcaaagact gtattggtgg caatcggcat
    10321 ggctgcaatg tccagcatcg ccccgcaagc ttttgcccag gaagacggat tagttggcgt
    10381 ggatatccga aatataaaga aggatattgc cgagagtatc aacgttgatc ccggcaagat
    10441 cccgtcagcg gttcgcgtaa aggcaaatat agcgacggaa acttgcaagg tgccggaaga
    10501 catccttgcc cagcaggaga cgaagggagg cgcgaactgc gtggccaaga tgacttcccc
    10561 agccctgaac cgactcgtag accgggaaat aagaggaatc acgaagccgt aactattcga
    10621 atcgcggtct cgcaatcgag ggttaaggga tcgaagtttc aatgatactt cgctctctgc
    10681 tgccgaattc gcaaaaatgc ccctgatcag cggctaaacc ggaatcaata gtagctgaac
    10741 tttatctgcg caaagtaggt gcgctgtggg aatgggtgaa aaatccagta tttgtcgtta
    10801 ttcacgttat tgatgccgcc gatcagggac acctgcttgg cgagctggta tttcgctacg
    10861 agatccacga cgacgtagct ggtgatcccg gtgtaggcaa atccgttggt atcgctattg
    10921 ttgagctgat tgaactggcc gctgctatag cgcacgctcg ccgttcctgt cagacgttcg
    10981 gttgcgcgat aagtcacaac ggcattggcg cgccacacgg gtactcgagg ctggcgcttg
    11041 ccagtcgagg gagaggcggc attcggattg gtcgccgtcg ggcccaacgc agctgcagcc
    11101 gcgtcggcgg cctcgttctt gacgattctg gagtctgtcc aggtaaaatt cccggtaacg
    11161 tcaagcccct ttacgaggaa atccgattgc tcgccagaaa attcaattcc gtaggtatcg
    11221 atctcaccca cgtttgaaat gaacgttgcc tgagaagtcg tattggttga cggattcgca
    11281 accacgcctg tttgggaaaa aatcgcgttt ttgacccgct cctggaatag acttaagcga
    11341 agccttccct tatcgcgaaa atattcaccg gacaattccg aggagagcgc ttcctcgcgc
    11401 ttgaggttgg ggttcgcatt gaaggagatc gcggtcgcac cagtgcccac cgtcgtggtt
    11461 tggaacaatt ccgatgcagt cggataacgg taagcctgcc cgatcgctgc gccaatcctg
    11521 acgcgttcca ttggcgtcca ggtcagcttt cccttcggcg aaaaattgac tgaacttctg
    11581 tcggcctggt tcacggtctt gagtcccgcg cccacggtcg tgatatttac accgtcatac
    11641 gcgtcccagt tttccacacg gccgccgaag gtgaagttcc agtcctggtg gaagtcccag
    11701 gcatcctgta tccagtagcc ctgagtctgg gtcttgccaa cggaacttga aaagattccc
    11761 tgccccccgc ctgtctgcca attgccggtg ttgtagaccg ggttcttgag agtgtataga
    11821 tcgtggtgga aaccgaagct gatctcgtgg tagccaagca aatctgttct cggccgccag
    11881 atccccttcg catccaccgt atgccagccc gacccggtta agctggtgat gcgtcccggc
    11941 gcattgatgg ctccggccag cgtcgggtca accgttggtg cacgtaccgt atcccttgcc
    12001 aggtcaacaa cgctgcctgt caattcccaa tcaaaaactc ctcccgtatt cgagcgcaga
    12061 ttgatgccat gcgaccaggt cgtttgattg gtgagattac ccgcgaaagt aggactattc
    12121 gcagcagaca ggttatatcg cctgccatcg atgttgacaa acccggactg caccatgttt
    12181 ccagccgcgt ctctcagata actctgaact cttgcagttg cgctgttttg ccacatccca
    12241 aaggtgtagg cgagcttgat agcaggggcg atgtcgtagg ccaacttcca cttgaaatta
    12301 tcctgcacag tatgattgat gtttcccgcc ccaagtacct gggtgggggc gttggtggga
    12361 ttcagggtgt caaaagctcc cgtaaccaac ctgtcgtccc cacccgcaat tgttccggag
    12421 ttgagtgccg tcgcgaacgt aatgggctgg ctatgggcgt ccaggtggct gtaatcgaag
    12481 cggaaagaca agtccttgta cttgtgcccg aagtagccca tgtaccgctg gctttcgtag
    12541 gtatccttcg tcccataaag attgaacgtc tgccaggatg actggacgtc gatgcccgct
    12601 tcaaacttct cgggcatctt ggtgatgatg ttgatgacgc cgccgatcga attgcctggg
    12661 taagccgcgg cgaagggtcc gtataaaacg ttgacctggg cgatttcgct cggggaaacg
    12721 gtgttccagc ggggtgaacc ggtgttctga ttgttattgc caagcaggga tgacagcagg
    12781 attccatcgg cgtagatcag gttgcgcgcg ctcgctgatg ttccggatgt gcgcatcccg
    12841 acgggcgcgt ttgtatcgcc gatatagcgt gtgcgcacct gaatggaagg cagatacttg
    12901 agcgcgtcct caggattggc cagattgatg gtcgcatcta ttttttcttt catgattgtt
    12961 tctgtcgtct gcggcagccg gtatctctcc ttgtccgtct gcttcgcagg cggcgtgacc
    13021 ttgcctccga ccgttatttc cggcatcttt acttcggatc cggcaaggtc tgttttctct
    13081 tccggcgcgt cgtgacgtgt cagtttgatt ggcgctgatg attcctgcct gaccggctct
    13141 tttaccctca gatcccgctc atcggcggcc ctggaaaacg atgcacaaga cacgaggaaa
    13201 aaagaataaa aaatgacttt tgcatgtccg gcgcgcattg cgatccccac tcgactggag
    13261 cgtgagatta atgcatttct ccacaatcag gaatgcggca aattgtcgca ccttaccgct
    13321 gatcttgacc tcgttcccga tcactgcggc atgtcggcta aagttcagct atgataaatt
    13381 ggaaaggaca ccatctatgt atttcaactg tctgaacatg aactctggaa gaattccgcc
    13441 tttgttgctt aatccggaaa gcgagaaggt cacgatcacg tgctcttatt gcgtgaatgc
    13501 atttgaagag atcgtctcgc gggtcatata tgaaccgaag gtttgctgcc cacgttgccg
    13561 gcacgtcgta aaagtcgatc ttcttcagct gaatctcatg cttgaaggga caagaaagca
    13621 cgccggcaac ctcttcaaga ggctggtgca gcgatcaggc ggcaggcgcc cggcgtaggc
    13681 aattcgtcga ccaattgaga gcggcaagct gagcgcgctg ccccctatgc gaacagtgtg
    13741 atgcaccggc caggggggtg gcatttttca cgctgacagc aatcgcggtt aagggggcgg
    13801 tagaactggc gagcagtcga attgacaagg catcgaaaag attaggccat accctcgcgg
    13861 gcgatgattc ccgaatgata gcggtgaccc gctaaacgga gtaggcgcgt tagtattggg
    13921 ccaggaagct tggagagggt gaaacggagt ggacgcggcg atcataatgc tccccgtgcg
    13981 ggcaaggccc gcgcgattag cggattaaat ggtcatcaac caagtagggc ttcagcggcg
    14041 agattacatg aacccgttcg actcagaacg gtaaacgccg tgggagcctt accagagagc
    14101 gctcatagca ctatgactct ttccagccct ttagcaaacc taatcaagaa ttggctggct
    14161 ttaaacacaa cagtttccga gcgctcgggt ttctgaatgt cggattcaag cctacatagc
    14221 aaatcatcca accgctcgtc actggccgca tcgccagtca atccaaacat atctactaaa
    14281 tttgattttt ccatttaccg gctctcttca ctcgttgttt aagacaagca aaccagcgga
    14341 cagttttatt gttccgagcg cgccgaagga aacttgtaca ggataatcat tacttacagc
    14401 gtaaatcatc tcattcccgc aacgaggctc tctgtacgat ggcgaacaga tgctccagtg
    14461 tgggaactga ctccagggga tgttgggggg atctagatgg aaatggccga cattgtgcca
    14521 gatgggaaga tcgctatgca gcggcggaaa tcacaggaga ttactctcaa ccggcatagc
    14581 gcgcgagtga cggaaaagcc gacggacagg caggcatttc ttccagctct tcagtaagcc
    14641 attaaacaaa aaggattaga acgcaagtgc gctaatcctt tgttggtagc gaatcccaga
    14701 ttcgaactgg ggaccaacgg attatgattc cgctgctcta accactgagc taattcgcca
    14761 cgtgtgtata agaaccgaaa attttgccat tcccagtcac gcctgtcaag atcataaaac
    14821 caccagattc tatatccatc tgatctttaa cggcgcttaa ccccatttta tctattcttc
    14881 ataactgggg tcgccggaga cgccagtccg attatttcgg attggccgcg ggttgccgtc
    14941 ctgtgggtta aaacggtctg tataatcgtc tttatgaaat tcgacgtggt tgtagtaggc
    15001 ggaggactgg taggagtcgg cttggcgctt gcccttgccc aagggagcgt caaagtagcg
    15061 ttgatcgagt cacgcgagcc cccccctctt cctgaagacg atagctggga tagcagagtt
    15121 tatgcaatca gccctggcag tgccgagttc ctgcaaaacc tcggtgtatg gcaggcgctc
    15181 gaccaagcac ggatcacgcc tgtgtatgac atggcggtgt ttggcgatga caatgcctcc
    15241 cgtattgatt tcagcgctta cgacatcgga tgttccgcgc tcgctttcat tgtggagaat
    15301 cgtcaacttc aggctgcggt atggaaaagg ctcaagcgcg aaagaaagaa tgtcacgcta
    15361 ctctgccccg cgcaatgtgc attgatgacg cggactgagt ctcaagtcca tttacatctc
    15421 tcggatggaa ccgagcttca ggcagctctg attgccggag cggatggtct taattcctgg
    15481 gtgcgcgagc aagcgggaat cgaaacgaag cggcagccct acgatcaaac gggagtggtc
    15541 gccaatttca aggctgagct gcatcaccgg aatatcgccc accagtggtt ccgtcgagac
    15601 ggggtgctgg cgctgttgcc actgccggat acaatggtat ccatggtttg gtccgcaaac
    15661 gaggagcagg cccgcaagct gctcgaatcg tcgcgtgaag acctgtgcaa tgaggttgtg
    15721 gaagcttcct gcggggtttt gggggatatg cagaccgtaa ccccgcctgc cgctttccct
    15781 ctgaatttcg tgcacgtgac caagctcgtg cagcctcgcc tggcgctgat cggcgacgca
    15841 gcgcatggta ttcatcctct tgcggggcaa gggatgaatc tggggttgcg ggatgcgaga
    15901 gagctggctg caataattct cacgcgaggg ttgcaggcgg attgtggaga ctatctgctg
    15961 ctgcgccgtt atgaacgcgc gcgaaaggag gatatcctgg ccctggaact tgctacggac
    16021 ggtttgcaga agctgttcac cgccacccac ccgacactca tccgcctgcg caaccttgga
    16081 ctggaaatga caaatcggct gccacttctg aaggaccggt tgatgcgcca cgcgttgggc
    16141 tagaactgtc gatttgcaga cggcaatgca ggattaagat tatttcaacc cgacagaatc
    16201 ggagtaacca cgacccatgc gttcaaaact agtccttccg ttccttttgg cgtgcctcgc
    16261 cttcggcgag gcctttgctg atgaagcttc cttgaagaaa gcgctgcagg ggatatttcc
    16321 ggacgaaaaa atcgaaagtc tgaaaaagac gccttatctg ggactgtatg aggtggtgat
    16381 cggaggcgaa ctgttttata cagatgacaa ggccagctat ctctttttcg gtcatgtagt
    16441 cgacccaaag acccggcaga gcctgaccag cgagcgcttg cagcagatca aggaagcgcg
    16501 ccgggtcaac caggactccc taccccttga tctggcaatc aaggcggtca agggagacgg
    16561 caaacgcaaa ctggtggttt tttcagatcc gaactgtcct tactgcaagc gccttgaaaa
    16621 ggagctcacc aacatcacgg atgtaacgat atacacgctg ctctacccag ttctgaatgg
    16681 ctcaataccg acggccacgg caatatggtg ctcctcggac cggctgaaag cctgggatga
    16741 cttcatgttg agagggacgg ccccaaccgg aaaaaattgc gaggctccta tagaaaagct
    16801 gcttgaggcg ggccgcaaga acgggatcag cggtacgccg acgctaattt ttgcagatgg
    16861 ctcggttgtg cctggaatga tcccgacgga tagcatcgaa aagaagctca gtagcgcgcg
    16921 gaagtaagag cccaggggtt ccgctaaaag gcgaatcagg cagtacctct gtaccggcgc
    16981 ggataattgg ttttaacgcg tccctgtatt tgtcgtatag cccaacggca tgagaaccca
    17041 catttttcct gtctgcctca ttttacctgc ctgactggct gcctccgccc cgaagcccca
    17101 gaaaaagtct gcgcgaacgt tccccttgat ggcgccgccc gtatcctggg ccaccatcag
    17161 tcgggaaagc ggttgagtcg tgtttggcca ggtagtggcc aggaaaaccg gggcgccttg
    17221 agggatagcc cgaggatcaa ttgcgaggct tctgccagcg gtcaacggta cgcccaacga
    17281 gccgagtggc cccggaatat tggcgggaag ttcacggaaa aatacgaagc tcgagttttg
    17341 ctgcaagagt tcggaaagct tgtgcgggtt cttttgcccc cactcccgga tgccctgcat
    17401 tgatgctttt tccagttcca gctcgcctcg ctccacaaga acctttccca ccgatttgta
    17461 gggatgtccg ttttgatcgg aatagccgac tctcacgatc tcgccatcct cgagctgcac
    17521 acggcccgac ccctggattt gcaggaaaaa cagatcgacc tcgtcatcga cccagagcag
    17581 ctccttgcct ttaagcgact gtggattgct cgtgatttca gcccggctgt aaaaaggtac
    17641 tacctttctt ccctgcaacc gcccgcgtag cctcatgttc ttcagttccg ggtacaaggc
    17701 gccgaggtca atgaccagga gttcatccgg cgttgtatag attgggtgcc ggtaacggcg
    17761 cgagggctgg cggcttccct tgagcagcgg ctcgtaatac cccgttatca gcccctcgcc
    17821 actcccgtcc ggattcaaaa cctcatgggg aacgaagcgg ctctcgaaaa attgccgtaa
    17881 atcatcactg ctcctcccct gaaaatagct ggcctgggtg caggtttcct gccatagcgg
    17941 ctggtttttc aatacgctgc aactacgcag gaaagcatcc catgcaggct gaatatcatc
    18001 ctccacccaa ccaactacag cgttccacgg ggcgggttgc agcacgacag gaggaggggt
    18061 ggggatgctt gacggtatgg cagcagccgg cttttcaggc gcagcaggag ctgtaggagg
    18121 acgactggca caggcatcaa gtaacagtaa tgcagcaatg gctgtaaaac ttaattggtt
    18181 tttcatcaca atttcgttaa agttgcattt cgcaaacata cagcaagatc aacccgaaaa
    18241 catgtgggta ttagcactgg aagcgttcgt cgctctgaca ctactgattc tcatcgtctg
    18301 gtggaccaag ccggccaaaa gaaatgacga ggaggaaaac gatgacgaaa agaaagagtg
    18361 acaagcctca cccgaatatt ttgcctcaac tgcggattct tggaaggttc gttctggccg
    18421 tcggcaacgc gtcggtaata acaattcgca ctacatagag acatagggcc acgccattcc
    18481 cgctgtctca cgccattccc gctttcttat accgcgcatt tctatcgcgt ataaatccag
    18541 tgcggttctc aaacgaattt ggcagaatga acaccttgca aacatggttt ttacaagggg
    18601 acaacaatga aatacaaaag ttttcaagaa ttctgccagt cgatcgcaga gcgaaatcct
    18661 ggccagtccg agtatatgca ggccgtcgca gaagtgatgg agagtctctg gccattcatt
    18721 acagaccacc cccgctacgc ggagcatggc atccttgatc gcctcatcga acctgagcga
    18781 gtgatcattt tccgtgtagc ctgggtagac gatcacggag aagtgaacgt aaacagggga
    18841 taccgtatcc agcacagctc atccatcggc ccttacaagg gcggtacccg ctttcaccct
    18901 tcagttaatc tttccattct caagtttctt gcgttcgagc agactttcaa aaatgcgctc
    18961 accacgctac cgatgggggg cggaaaggga ggcgccgact ttgatccgaa gggacgctcg
    19021 caaggggaag tcatgggctt ttgccaagct tatatgaccg agctttttcg ccatataggc
    19081 tcggacaccg atgtgcccgc cggggatatc ggagtcggcg ggcgtgaagt cgggtacatg
    19141 gcaggaatga tgaaaaagct gtcgaaccga tcggattgcg tgtttaccgg caaggggctg
    19201 agcttcggag gctccctcat tcggccggaa gcaacaggat acggcacagt gtattttgcg
    19261 gaggaaatgc tgaagcagtc cggccgatcg ctcgccggcc tgcgcgtttc cgtttcgggg
    19321 tcaggaaacg tcgcgcaata tgcagtggaa aaagcgatgc tgctgggtgc aaaagtggtg
    19381 actgtgtcgg actccggcgg cactgttgtt gacgaagagg ggtttaccag cgaaaaactg
    19441 gctgagctga tggacgtcaa gaatcacctg cgcggacgta tcagtgagta tgcagaacgt
    19501 gtaggagcag atttcctgcc cggcgtgcgc ccctggcacg tgccggtcga tgtcgcgctg
    19561 ccctgtgcga cccaaaacga gctcgatgat gcagacgcgg cgaccctgat ccagaatggg
    19621 gtcatttgcg tcgcagaggg cgccaacatg ccctcgactg cagaggcggt aaagcgcttt
    19681 gaacaccagg gggtgctgtt tgctccaggc aaggccagca acgcgggagg cgtggcgaca
    19741 tcaggacttg agatgagcca gaacgcaatg cgtttgtcat ggccgcgaga agaggtggat
    19801 gcccgcttgc tggagatcat gcaagccatc catcaatcct gcctgagata cgggagacgc
    19861 aaggatgggt ccgtgagtta tgtggacgga gccaacgtag cgggctttgt aaaagtggca
    19921 gacgcaatga ttggccaggg agtgatctga caacgagata gaatggcagc agcatcctat
    19981 taagccgcgg cactccgatg atccttgacg attatagctc ttgcccggcg tcaggatgat
    20041 ttcttttccg cctttcacaa cctagctaaa tattgctacc cattctgaaa ccgttcgatc
    20101 cacaaaaaca gctatgggtg gaccgcttac tgtgtgagtt gccaatcagg tgagaagctg
    20161 attcagccag ctaagtttgg cgatgtgttg ccaagccagg atgccgacga ctacgccaaa
    20221 gaatggacag gagggacaac gagttgcgcc gactcatcag ttagtccgct gcaaaagcca
    20281 gtcatggtcc atctttgcgt cggaatagct taatgcaatg caagcaaggc gtgcaactgg
    20341 gcaacgactg ccgcaccttc acccacggcc gcggcgacac gcttgattga gttggcacgt
    20401 gcgtcgccga tcgcgaatac cccaggtgcc gaagtttcga gctgtagggg ttgaacacgg
    20461 ctattttccg aatgcgccca ctgctcacgg tcggcaggcg agagatccag gcctgtgcat
    20521 acaaacccct tgccatttaa tttcacaccg caattctcaa gccacccggt gttcggatcg
    20581 gcccccacga acaggaaaac gcggcgggtt ggcatcgtct gcatgctgtc attttggcga
    20641 ttgcgccaat gaacggcgct caccccttca tcgcagcctt cgatcgcaac gatttcggat
    20701 tccgtatgta aggtaacatt cgccagcgac ccaatagggt cgatgagata ttttgacatg
    20761 ctttttgcca gattggcccc gcgaatcaag tgatgcacgt gcgccgcgtg gctcgccaag
    20821 aatactattg cctgtccagc cgaattgccg ccgccgacca gcaccacttc ttcctctcgg
    20881 catagcgccg cctcgatcgg cgaagcccag taatagaccc cgcgaccttc gtatttatcg
    20941 agttcgggta tgcccggacg ccggtatctt gcacccgtgg cgataaccac ggtttttgaa
    21001 gaaaccttga caccacaatc cagtttgagc acgagcggaa agcgggagca atcgagtgac
    21061 gtcacttcga cggggaccac catttcggca ccgaatttct gggcctgaac ataagcacgt
    21121 cccgcgaggg cctgaccgga gataccggtc gggaaaccaa ggtaattttc gattctcgca
    21181 cttgcgcccg cctgaccacc gaatgcatag gtgtcgagca cgagtacgga aagcccttcc
    21241 gatgcggcat ataccgcagc ggcaagaccg gcggggccag cgccgacaat cgccgcgtcg
    21301 tagacccggt cagcgctcag atcgatatac aagccgaggc gacgtgcaag ttctctttca
    21361 gtggggcatt taaggaccgt tccatcggga caagcaacca acggcaattc atttggacgc
    21421 ggctgatgga gcttcatcac atcttccgca acactatcaa gtgccggatc cagtactaga
    21481 tggggatggc tattccttga aagaaatccc tgcaaccgca ccatatctgc cgcaccaggg
    21541 ggaccaatca gtagcggtcc gcctccgccg gtttcgacca atgccatgcg tctcaggatc
    21601 agggctctca tgatacgttc cccgacctcg gcctcggcca cgaccactgc gcgcaaggag
    21661 gctggatcta tcatcaatgc ttcaaccatc tcaagggcac gcccataggc aagaggagga
    21721 cgacccgaaa gctgtccaac ttcgcccgtg aagttgcccg gtccgctctc aattatggga
    21781 atctcgatgc cgcagccatt tttgcgtgtg gttagaatgt gtccatgtag caaaacgaac
    21841 atgcccgtcc cgggcctgtt gggttcgaat aatagttcac ccgcctgcca cgtgcgaacc
    21901 gtgccaaaac gccttaaccg tgtaatctct gccggagcga gcttgggaaa catctgctcg
    21961 cggcgtggcg cgagggttgg cggcaatgaa aaatctgctg cgttgggtgg accagcgttc
    22021 acttgaccag acgttggcgg gagcaaacta tcagggattt ttttcatcgt agcttgggtg
    22081 acctccacaa ggaatcacgc tgaaggaagg aatgatggct cagcaaacta atccaaatcg
    22141 tatgacctac ggcccggggt tttgtccgcg ctgcttgtcc gcgttccata agatggccac
    22201 gccgaggatc gccgatgcta ttgaggcggc aaacaccgcg atcttggctg cggcaaaatc
    22261 agtcgctgtc gggaatgcct gactggctat aaacagcgac atggtaaagc cgatccctgc
    22321 cagtgcgcca gctccgatta attgtcgcca cgaatattca tcaggcttgg ttgcgattcc
    22381 gaaccgcaca gcgatgacag atgccaacac gagtcccagg ggcttgccga ttaccaatcc
    22441 gacgataacc gcaagcataa gagggtcgtg gccgatgaga gcctcccccg taaccgcgac
    22501 tcctgcatta gccaaggcga acagcggcag cacgaggtag ctcgaggggc cgccggcatg
    22561 tcgcagcagg cgatcggctg gcgattccag gcgatcgtgg attgcatcga gcgctcgcat
    22621 cgccggaaga gaaggaccgt gccgcaagac ctcgctgcga cgccgtgctt cagtgctcag
    22681 gatcgcgtcc gcttgcacca tcaacgccgt cagattcggg ggcggcctcg tcggaatgaa
    22741 cagtgccagg atcacgcccg ccatagtggc gtgaagcccg cttgcgagca cgcaggccca
    22801 gagcaggatg cccagaagca cgtagggcgt gacgttgtaa atgtgggagc ggttgaggag
    22861 ggccagcgcc gcaacaatgg ccgccgctcc cgcaagatag ccgaagctca tcgtgccgga
    22921 gtagaagatt gccacgacga tgatcgctcc gatgtcgtcg acgatcgctg cggccgtcag
    22981 gaagatgcgc agttcggccg gcacgcggtt tcccatcatc acgatcagcg ccacggcaaa
    23041 cgccgtgtcg gtggccatcg gaacgcccca cccgtgtgac cagggtcccg caggcgccag
    23101 tagcaggtaa aggatcgctg gcgccaccat gccgccgatg gcggcggcaa ccggg
//
LOCUS       DASSHJ010000106         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_778066, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000106 DASSHJ010000000
VERSION     DASSHJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778066"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..2715)
                     /locus_tag="VFI43_04265"
     CDS             complement(340..2715)
                     /locus_tag="VFI43_04265"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET7061373.1"
                     /translation="MKSNGKNEDDNRSTIGGPAHGKGNPANKYTAKLAATEELVVSMP
                     YDVNKPHEYGKDSVSPEKGQTVEPPHPSVTGSTLTETIPSEKTGRGDLQLGINPGNES
                     LDRVRSDSSGRVLTTNQGVPIGDNQNSLKAGLRGPALLEDFILREKLTHFDHERIPER
                     VVHARGSAAHGYFECYKSLEQYTRASLFAEAGKRTPVFVRFSVVFAERGGADAVRDAR
                     GFATKFYTDEGNWDLVGNNIPVFFIQDSMKFPDLVHAIKPEPHTGFPQASTAHDTSWD
                     FISLMPESIHMVLWTMSDRAIPRSYRMMQGFGVHTFRLVTTDGASQFVKFHWSPLAGT
                     HSLTWDEAVKINGADPDFHRRDLWEAIESGAFPEYELGMQIFTEAQAEKFSFDVLDAT
                     KLVPEELVPVMPVGKMVLNRNPDNFFAETEQVAFCAAHVVPGMDFSNDPLLAGRIHSY
                     VDTQLLRLGGPNFHEIPINSPVAQVHNNQRDGFHRQSINRGRVSYEPNSLAGGCPFQA
                     GTAGFTSFPQPVEGDKVRGKPERFADHYTQAKLFWNSQTQLEKDHIVNAFRFELGRVQ
                     TPAIRERMVSILVNVADDLAQSVAQGLGMNGLPAAQPKALDQPAIPEVERSPALSLFA
                     RPGDGSIQTRRIAIMVADGVDGVAVNALRDGLTEEGAVPRFIAARLGTVQSMEGSSIE
                     VDVTFEVMPGVLFDALAIPDGSEDMKDLAGMGEVVEAIREQYRHGKPILALGTGIDLI
                     KNAGVKIALPSGNPDPGVLTFPEYSDDVLPQFIEAIAAHRHFERETDPPRV"
     gene            complement(2929..3330)
                     /locus_tag="VFI43_04270"
     CDS             complement(2929..3330)
                     /locus_tag="VFI43_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7061374.1"
                     /translation="MLYLLFFLLRDRVALSARIRQGISLSQEHKGNLFGKFTIVVRAT
                     VKGNIAVAAIQGTLGGVIFSFLGIQGALLWGFVMAFLSLLPAIGAGPILGPVAIYFLL
                     TGAIWQGITLVIFGVLVIGTVNNVLRPILVG"
BASE COUNT          887 a         1025 c          934 g          855 t
ORIGIN      
        1 ggtcctgggt tatttcaaca tccagcttgg atacgttctc ggcaatggta ccgatacggg
       61 gttcaaattt attcactgaa tcacgtcaac gcctcgcatg aaattcctgt cttcccaaat
      121 tcaatagcca aattatacca ttcgcatagg aagggcgggg tttttcaaca tcctgttaaa
      181 ggattcatgg attcgtcgat actctacaac caccagctaa ggctggtggg ttagatgtag
      241 tttctcacaa cgttgttcac ctgaagccga tgcgtgccat tcccattcaa acacacggcc
      301 cagcgcactt cgcttattgc gccctctgat aactgtttgt taaacacgag gaggatctgt
      361 ttccctctcg aaatggcgat gggccgcgat tgcctcgata aattgcggca ggacatcgtc
      421 ggagtactct gggaacgtaa gcactcccgg atcagggttc ccgcttggca gcgcgatctt
      481 tactcccgca ttcttgatca ggtctatccc ggtccccagc gcaagtatcg gcttgccatg
      541 ccgatactgc tccctgatag cctcgaccac ctcgcccatg cccgcgagat ccttcatatc
      601 ctctgaacca tcgggtatcg caagcgcgtc aaaaagcaca ccaggcatga cttcgaacgt
      661 gacatcaacc tcgatgctgc taccctccat actttgcaca gttccaaggc gagcggcaat
      721 gaagcgcggc acggcgcctt cttcagtcag gccgtcccgc agcgcgttga cagccacgcc
      781 gtctactcca tccgctacca tgattgcgat gcgccgcgtt tgaatactgc catctccggg
      841 ccgggcaaaa agcgagagtg caggagacct ctctacctcc ggaattgccg gctggtcgag
      901 cgccttgggc tgggccgcgg gcagtccatt catccccagc ccctgtgcta cgctttgcgc
      961 tagatcgtcc gccacgttta ctagtatcga taccatgcgc tcgcgtatcg caggggtctg
     1021 cactcgtccc agctcgaaac gaaatgcgtt gacgatatgg tccttttcaa gctgcgtctg
     1081 actattccag aataatttcg cctgggtgta atgatccgca aaacgctcgg gtttcccgcg
     1141 caccttgtcc ccctccaccg gttggggaaa cgacgtaaaa cccgccgtcc ccgcttggaa
     1201 ggggcatcct cccgccaggg agttgggctc ataggaaaca cggcctctgt ttatggattg
     1261 gcggtgaaag ccgtcgcgtt ggttgttgtg gacttgcgcc acaggtgaat tgatgggaat
     1321 ctcatgaaaa ttgggccctc caagtctcaa taactgggta tccacatatg aatggatacg
     1381 ccctgccaga agcggatcat tgctgaagtc catgcccggc acgacgtgcg cggcacagaa
     1441 agctacttgc tctgtttccg caaaaaagtt atcgggatta cggttgagaa ccatttttcc
     1501 aacgggcatt actggcacca gttcttctgg caccagcttc gtagcatcga gtacatcgaa
     1561 gctgaatttt tctgcttgtg cttcggtgaa gatctgcatg cccagttcgt actctggaaa
     1621 cgccccgctt tcaatcgctt cccagaggtc gcggcggtgg aaatccgggt cggccccgtt
     1681 gattttgacc gcctcgtccc aggtcaacga atgcgtgccc gcaagcggtg accaatgaaa
     1741 cttgacaaac tgcgaagcgc cgtcggttgt taccaggcgg aaagtatgca cgccgaagcc
     1801 ctgcatcata cggtagctgc ggggaatcgc acggtcggac atggtccaca gcaccatgtg
     1861 aatcgattcc ggcatcagcg agataaaatc ccaagaggtg tcgtgcgcgg ttgatgcctg
     1921 cggaaagccg gtgtgcggct cgggtttgat cgcatggacc aggtcaggaa atttcatcga
     1981 atcctgtata aaaaacaccg ggatattgtt acccaccaaa tcccagttac cttcgtcggt
     2041 atagaattta gtcgcaaacc ctcgtgcatc gcggactgca tcggcccctc cgcgttcagc
     2101 aaagacaacc gaaaagcgca cgaaaacggg ggtgcgcttt cccgcctcgg cgaacaggga
     2161 agcgcgcgta tattgctcca aagacttata acattcgaaa tagccatgtg ccgccgaacc
     2221 tcgggcatgt accacgcgct cgggtatacg ctcatgatcg aaatgggtaa gtttttcacg
     2281 cagaataaaa tcttcgagca gtgcgggtcc gcgaaggccc gccttaaggg aattctgatt
     2341 gtctcctata ggaacaccct ggttggtcgt caatacgcgt ccgctggaat cgctccggac
     2401 ccgatcgagc gattcattgc ctggattgat tcctaactgg agatcacccc tgccggtttt
     2461 ctcggaagga atggtttcag ttaacgtact gcccgttaca gaaggatgag gaggctccac
     2521 cgtctgccct ttctcgggag agactgagtc cttcccgtac tcatgcggct tgttaacatc
     2581 ataaggcatg gataccacga gctcttctgt tgctgcgagt ttggcagtat atttattagc
     2641 aggattccct ttcccatgag cggggccccc aatggtcgag cgattgtcat cttcgttttt
     2701 accatttgac ttcatatttt tctccttgcg ttgatgcctg acagcggccg ataccagtcc
     2761 aaaaggatgt gtctttactt cagggcgttc tgtacgtcat cacacgcaac tccaaaccta
     2821 gcacccctaa gtgcccgtag gcgcaaaagt taccagggaa aatgccctta ccgcgagcat
     2881 gagattaaaa aatcaaaagg acgtatccag ggtgggtatc cgggtaccct agccgacgag
     2941 tatgggccgt agaacgttat tcaccgttcc aattacaagt acaccaaaga taacgagagt
     3001 tattccttgc caaatggctc cagtaagcag gaagtaaatc gctaccggtc ccaagatcgg
     3061 cccggcgcca atagcaggca ataaagacaa gaaagccatc acgaagcccc atagcagggc
     3121 tccctgtata cccaagaatg aaaatatcac tccccctagc gtgccttgta tcgccgccac
     3181 ggcgatattg ccctttacgg tggcacgaac tacgatggtg aatttaccga ataaattccc
     3241 cttatgctcc tggctaagcg aaattccctg tctgattctc gcagacaagg cgaccctgtc
     3301 ccggagcaag aaaaacagca gatataacat gattccgaaa ctgacaacaa gctcaaatgt
     3361 attctggcca agctggaggg cctcctttct cctgcaatag tgaagcagcc atgaatgcga
     3421 acgggagaat caccatgatc aggcagagca atagggttgc gagcgcggca tgattctgcc
     3481 gcccgtgcag gcgccctgcc agccagcgat aaaatgtcac gaatattatg gcgagaacgg
     3541 agcctcgaaa tattgcgcca aaaaagggca gcaatatcca cccgaatgcc agggagacga
     3601 caattaacag gagcaggaag gctttttgct gaagctggag gggagttcat agatttgtgt
     3661 gcttttcaat tatcggaatt gttatttgac atgaaaccat a
//
LOCUS       DASSHJ010000107         8876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_782558, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000107 DASSHJ010000000
VERSION     DASSHJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782558"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VFI43_04275"
     CDS             complement(<1..1267)
                     /locus_tag="VFI43_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET7061375.1"
                     /translation="MRARILAFVLGVILLQQQAELPDLVRASLLLPGAALAFLLRHHS
                     RPVFAMTNKLLSCVVLLGAGFFWAALFAHHRLADNLPTEWEGRDIQVIGVIAELPQIN
                     GRNTRFAFDVEQVLETDASVPARVSLAWYPDKDKGANDGLPAIHAGERWQLRVRLKRP
                     HGSVNPHGFDFEEWALERNIRATGYVRKSSGNVRLDDMVDRPAYRIERVRQEIRERFM
                     EALPGHAYAGVLAALAVGDQRAIPAEQWQVFTRTGINHLMSISGLHVTMVSGLVFALV
                     YAAWRRSSHLTLWLPGRKAAAILGLIAALGYALLAGFAVPAQRTVCMLAVVALALLSG
                     RVTSATAVLALALLAVTFVDPWAVLSPGFWLSFGAMAVIMLVSTGRIGRIHWLSDWAR
                     IQWAITLGLIPALLAMFQQISLVSPIANAI"
     gene            1429..1983
                     /locus_tag="VFI43_04280"
     CDS             1429..1983
                     /locus_tag="VFI43_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET7061376.1"
                     /translation="MIRKFFKKYLPSHEVIRQNRFVALFGDRLQHHNLWHLHRRSVAG
                     GVAAGLFAGLIPGSNPVQFFFAALFAVMFKVNLPVAVATTLYSNPFTIVPIYIAAYAI
                     GEFVIGNGAEGNLPPDELHLMDKSISEWVPALVDWMVSQGKPLLLGLLLLALVLSFVG
                     YFVVRAAWRLYAVYAWRKRAKRRR"
     gene            2202..2819
                     /locus_tag="VFI43_04285"
     CDS             2202..2819
                     /locus_tag="VFI43_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET7061377.1"
                     /translation="MIPAYWDEAKRELGESDEVMRELTVRFDGAPLRSRGDAFSTLAR
                     SILGQQISVKAADSIWKKLTQTVGVVAPDALLGAEQNVLRDCGLSLRKIGYVQDLSAR
                     FSDGTLDASTFHEMDDEALIDMLTRIKGVGRWTAEMFLIFHMLRPDVLPLDDLGLQRA
                     LSMHYNSNEPVSRLKMRSIAKPWQPWRSVATWYLWRSLDPIPVEY"
     gene            complement(2830..3510)
                     /gene="lolD"
                     /locus_tag="VFI43_04290"
     CDS             complement(2830..3510)
                     /gene="lolD"
                     /locus_tag="VFI43_04290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HET7061378.1"
                     /translation="MSEPVISCQGLSKSYYQGKIEVPVLFGIDLTVAAGETLAIVGAS
                     GSGKSTLLHLLGGLDAPTSGYIRLMDRDLTSIDEEERCRLRNRSLGFIYQFHHLLPEF
                     TALENVAMPLLIRRMSSSEAYGYAASILEQVGLGPRLTHTPGELSGGERQRAAVARAL
                     VTRPACVLADEPTGNLDRHTAAGVFQLMLDLNRSAGASLIIVTHDYELARKAGRILRL
                     QDGTLVQE"
     gene            complement(3503..4750)
                     /locus_tag="VFI43_04295"
     CDS             complement(3503..4750)
                     /locus_tag="VFI43_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET7061379.1"
                     /translation="MSPYELFIGLRYTRAKRRNHFISFISLISLLGITLGMTALITVM
                     SVMNGFQKEVRARILGVASHIQISGLDGGLADWRLVAREALRHPEVEAAAPYVSSQGM
                     ISFNNVVSGVVVRGILPDMEDHVADLGRMMIDGKLEDLAPGGFGIVIGRELARALGAF
                     IGDKLVLISPQGQVTPAGMLPRLKQFTVTGVFEAGHFEYDSGLVLIHLADAQKLYRME
                     NDEVSGVRLKLRDMFKAPEVAYELLPMISAPVLISDWTRQHANYFRAIQIEKRMLSLI
                     LALIIAVAAFNIVSTLVMAVTDKQSDIAILRTLGASPRSIMTVFIVQGTLIGIIGTAL
                     GVLGGVLLAYNVGAVVAGIEKLFHVQFLSREVYYISEIPSDPQLADIVTVAVVSFVLT
                     LLATLYPSYRASRVNPAEALRYE"
     gene            complement(4925..6907)
                     /locus_tag="VFI43_04300"
     CDS             complement(4925..6907)
                     /locus_tag="VFI43_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7061380.1"
                     /translation="MFALITGTASAQAEDSGNTLQPVVVTATPFSNQSELEMAQPVSV
                     LKGDDLRRKREASLGDTLSRELGVASSSFGPGAGRPIIRGLDGPRIRVLDNGIGTLDI
                     STVSPDHAVTAESLNASQIEILRGPATLLYGSGASGGVVNVVNGRIPDKRMKSPKGNI
                     ETRGNTATEERTGALNVSGSLGKMSWSAGGFRRKTGDYSIPGFANKEDPAGKKGVVEN
                     SAVNSGGGYLGGSFVGEKGFMGVSVSRLDSKYGIPSPERSRIDLGQTRYDLAGELNEP
                     LRGFERLKVRVGYNDYKHNEIEGTGAIATRFRNQALESRAELLHAPLAKWQGVFGFQF
                     LYRNFSALGEEAVVPVTKSDSAGLFLVEERNWDRWRVELGGRIERSTRDPRQGTNPSR
                     AFNLYNVSAATVWKFMDGYRLTLSGTRGQRAPAAEELYIHGAHRGTATFQTGDPRLTS
                     ETTNNIDLALRKTDGNIKWKLNFFHSWIDNYIFVRSADTNGDGVPDRSDDHGVLDPNG
                     AFLVQNFTQRAARFYGAEAEAIVTLKPDAVDLRLFADYVRGKLESGGNLPRITPARFG
                     LELNHRMGPWTTNVSASHVMRQTHVAELETSTPGYTLVNVEATYLIKKTKSNGIRIFL
                     QGKNLLNEEIRVHTSFLKDFAPLPGRALVAGLRGDF"
     gene            complement(7165..7539)
                     /locus_tag="VFI43_04305"
     CDS             complement(7165..7539)
                     /locus_tag="VFI43_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061381.1"
                     /translation="MSAHRLLLLLRAFSALLLFALVLVPIFQSAHMLTHIRTPDSLHD
                     AVLVAQTGGVGAGEDGEDADSGLDKICLDCLALAAFSVALLTLAVLFFGAIRRLHLWH
                     LNSRGHFLDFYTPYLSRAPPQA"
     gene            complement(7536..7958)
                     /locus_tag="VFI43_04310"
     CDS             complement(7536..7958)
                     /locus_tag="VFI43_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET7061382.1"
                     /translation="MSRGFTKQAEEIILGRAERVTRGRVQVLAMLLEEQRAVSHHELE
                     QRFKGKHRLDRVTLYRVLEWLNERGYIHRVVGDDRVWRFRANFDLKPHQHAHFECTRC
                     TRVICLDDLKAEYDPPLPAGYRSQEVELRVRGLCPGCV"
     gene            8205..8528
                     /locus_tag="VFI43_04315"
     CDS             8205..8528
                     /locus_tag="VFI43_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HET7061383.1"
                     /translation="MPKIRSKRLRKKLHLDEFQEFGFLISFTLPQNLDNEALDIFFDR
                     FIDVIEHNGLLFGGGIGSNSEGFVTLDKRGNATEEYRELVRKWLALHPLVSGIQIWEL
                     VDAWV"
BASE COUNT         2050 a         2477 c         2421 g         1928 t
ORIGIN      
        1 ctatggcatt tgcaatcggc gaaaccagtg aaatctgctg aaacatcgcg agaagagccg
       61 ggatcagccc aagcgtgatt gcccattgta tgcgtgccca atccgaaagc cagtgtatcc
      121 tcccgatacg ccccgtggaa accagcatga ttactgccat tgctccgaat gatagccaga
      181 agcccgggga gagcactgcc cagggatcaa cgaacgttac ggcaagcaat gccaaggcca
      241 aaactgccgt ggcggaagta acacgccccg ataacagggc gagcgcgacc acggcaagca
      301 tgcagacggt tcgctgcgcc gggaccgcga aacctgccag cagggcgtac ccgagtgccg
      361 cgatcagtcc cagaatggct gcagccttgc gacccggcaa ccataaggtc agatggctgc
      421 tccttcgcca tgctgcgtac acaagggcaa aaaccaggcc tgaaaccatg gtcacatgca
      481 gaccggaaat actcatgaga tggttgatgc cggtacgcgt aaatacttgc cattgctcgg
      541 cgggaatagc gcgctgatcg ccgaccgcga gagcagccag aacgcctgcg taggcatgac
      601 cgggaagggc ctccatgaaa cgctcccgga tttcctgacg cacgcgctcg atcctataag
      661 ccggccgatc caccatatca tccagccgta catttccaga ggatttgcgc acatagccgg
      721 tagcccgtat gttgcgctcc agcgcccatt cctcgaaatc gaagccatgc gggttgacac
      781 tgccgtgagg ccgcttgagc cgcaccctca attgccaacg ctcacctgca tggattgcgg
      841 gtaggccatc gttggcacct ttatccttat cgggatacca ggcaagggaa acgcgtgcag
      901 gtacgcttgc atcagtctca agcacctgct ccacgtcaaa agcaaagcgc gtgttgcggc
      961 cgttgatttg aggcagttcg gctataacgc cgatcacctg tatatcgcgc ccctcccatt
     1021 ccgttggaag gttgtcggcg aggcggtgat gggcaaaaag ggcagcccag aaaaatccag
     1081 cgccgagcaa gaccacgcaa gacaggagct tgttcgtcat tgcaaaaact ggccggctgt
     1141 gatgccgcag caggaacgcc aaggccgcgc cgggcaacag cagcgacgcc cgtaccaggt
     1201 caggcagctc ggcctgctgt tgcagcagta tcacgccgag cacaaatgca agtatgcgtg
     1261 ctcgcacgtc agttacagtg accgcaattc atgcatgagg gtttgaaatg aagcgggtga
     1321 ggctgattga agcaggataa gcttggagaa ccactaatga gccggggaat agagcgtgat
     1381 agatggttat gtttgcaccg gccaccggtg tttccgtaca ctccccccat gatccgaaaa
     1441 ttctttaaaa agtatcttcc ctcgcacgag gtcatccggc agaaccgctt tgtcgcgcta
     1501 ttcggagatc gattgcagca tcataacctg tggcacctgc accgccggtc ggtggcaggc
     1561 ggggtggctg cgggactctt cgccggcctg atcccgggaa gcaatccggt ccagttcttt
     1621 tttgcagcgc tgttcgccgt catgttcaag gtcaaccttc cggtagcggt cgcgacaacg
     1681 ctttattcca accctttcac cattgtcccc atttatattg ccgcctatgc aatcggcgag
     1741 ttcgtcattg gaaacggtgc cgaggggaat ttgccgccag atgaactgca tctgatggat
     1801 aagagtatca gcgaatgggt acctgcactg gtggactgga tggtttctca aggcaaacct
     1861 ctgctcctgg gcttgctcct gctggccctg gtgctttctt tcgtcggcta tttcgttgtt
     1921 cgcgcagcgt ggcgcctgta tgccgtttat gcatggcgca agcgggcaaa acgccggcgc
     1981 taggcaattt cggggaaaag caaaagcact cgcagcgatg aatcaataga agcgggcttt
     2041 aagagcgcaa atcacagtgt cgatctacct gtaaaatctt tcaggtggcg ggcaactgct
     2101 tttcattgtt taatggcggc actgcggaat agctgagttt tcttacatcg ggtgcccaag
     2161 acgtcttgca gcaaatgaat ttgccgaacg atcagcatca tatgattcca gcatattggg
     2221 atgaggctaa gcgcgaactg ggcgagtcgg atgaagtcat gcgcgaactg actgttcgtt
     2281 ttgatggagc accgttgcgt tcgcgcggcg atgcgttttc cacgctcgcc cgttcgatcc
     2341 tgggccagca gatttcggta aaggctgcgg atagcatctg gaagaaactg acgcagacgg
     2401 ttggcgttgt ggcacccgac gcgctgcttg gcgccgaaca gaacgttttg cgggactgtg
     2461 ggctttctct gcgaaagata ggctacgtgc aagatttatc cgcacgtttt tccgatggga
     2521 cgctggatgc gtcgacattc catgaaatgg atgatgaggc gctcatcgac atgctgacgc
     2581 gaatcaaggg cgtggggcgc tggacagcgg aaatgttcct gatctttcac atgttgcgcc
     2641 cagacgtgct gccgttggac gacctcggct tgcagcgcgc attgagcatg cactacaact
     2701 cgaacgagcc cgtcagccgg ctaaagatga gatcgattgc aaaaccctgg cagccatggc
     2761 gttccgtagc aacatggtac ttgtggcgaa gtcttgaccc tatacccgtc gagtattgaa
     2821 cacgatcatc tattcctgga ccagcgttcc atcttgcagg cgcaggattc gacccgcctt
     2881 tctcgccagt tcgtaatcat gagtcacgat aatcaggctc gccccggcac ttctgttgag
     2941 atcgagcatc aactggaaaa cacctgcagc cgtatgccga tccagattcc ccgtaggctc
     3001 atccgcgagg acacacgccg gccgggtaac gagcgcgcgg gcgaccgcgg ctcgctggcg
     3061 ctcgcctccg gagagttccc ccggagtgtg ggtcaatcgg gggcccaagc cgacctgctc
     3121 caggattgat gcggcgtatc cataggcttc gctgctgctc atgcgccgga taagcaaagg
     3181 catcgcaaca ttttcaaggg cagtgaactc gggtagcagg tgatgaaact gataaataaa
     3241 accgagggac cggttacgca acctacagcg ctcttcttca tcaattgagg tgagatcccg
     3301 atccatcagc cggatgtacc cgcttgtcgg cgcgtccagc ccgccgagca ggtgcagcag
     3361 agtgcttttg cccgaccccg atgcccccac gatcgcgagc gtttcgcccg ctgcaaccgt
     3421 cagatcgatt ccaaagagta ccggcacttc aatcttgccc tgataatagc tcttgctcag
     3481 gccctggcag gaaatgaccg gctcactcat agcggagcgc ctccgctggg ttgaccctcg
     3541 atgcccgata actggggtaa agcgtggcaa gcagtgtcaa tacaaaagag acgaccgcca
     3601 cagttacaat atcggcgagt tgcggatcgg aggggatttc gctgatgtag tagacttccc
     3661 gtgacaggaa ttgcacgtgg aaaagctttt cgatcccagc cactaccgcc cccacgttgt
     3721 aggcgagcag cacgccgccg agcacaccca acgcggtccc gatgatgcct atcaaagtcc
     3781 cctggacgat gaacaccgtc atgatgctgc gcgggcttgc accaagcgtg cgcagaatcg
     3841 caatatcgga ttgcttgtca gtaaccgcca tcacaagggt cgatacgata ttgaaggccg
     3901 caaccgcgat aataagggcc aggatgagcg acagcatgcg cttctcgatc tgtatcgccc
     3961 gaaaataatt cgcgtgttgc cgggtccaat cgctgataag caccggagct gaaatcatgg
     4021 gaagcagctc atatgcaacc tcgggcgcct tgaacatgtc gcgcaatttc agccgcacgc
     4081 ccgacacctc atcgttttcc atacggtaaa gcttttgcgc atctgccaga tggatgagca
     4141 ccagccccga gtcatactcg aaatgccccg cttcaaaaac tccggtcacg gtgaactgct
     4201 tgagacgtgg gagcattccc gccggcgtca cctgcccctg cggagagatc agcacgagct
     4261 tgtccccgat aaatgccccg agcgcgcgcg cgagttcgcg tccgataacg atgccgaagc
     4321 cgccgggcgc caagtcttca agcttgccgt cgatcatcat tcggccaagg tctgcgacgt
     4381 ggtcttccat atcgggcaag atacctctta caacaacgcc gcttacgacg ttgttgaatg
     4441 agatcatgcc ctgggaactg acgtaaggcg cagcggcttc cacctcagga tgcctgagcg
     4501 cctctctcgc aaccaggcgc cagtcagcca gccctccgtc aagtccactg atctgtatgt
     4561 gagatgccac ccccaggata cgcgcgcgaa cttccttctg aaacccgttc atgactgaca
     4621 tgacggtgat gagtgcggtc atcccgagcg taatgccgag cagggaaatc agcgagatga
     4681 acgaaatgaa atgatttcgg cgcttcgcac gggtgtagcg gaggccgatg aagagttcgt
     4741 agggggacac gagtgaacat atgaaaagac aaccctgaag tttgccacat tcagcctggc
     4801 cttagtaaat aactggctct ggggagagat tgcgaactgc ccggccgttc aaggaaggcg
     4861 gatactggta aaacggtgca tccgccaatc acctggacaa tggcgacgat ttccggtcat
     4921 cccatcaaaa atctcctctt aaacccgcca caagcgccct tccgggcaag ggggcaaaat
     4981 cttttaaaaa ggaggtatgc acccgtattt cttcattcaa aaggtttttt ccctgtaaaa
     5041 aaattctgat gccattggac ttggttttct tgatgaggta agttgcctct acattcacca
     5101 gtgtgtagcc gggcgtgctt gtttcaagct ccgctacatg agtttggcgc atcacatggc
     5161 tggcgctcac gttggtcgtc cagggaccca ttctgtgatt caattccagg ccaaaccgcg
     5221 ccggagtaat gcgcggaaga tttcctccgc tttccagttt tccacgaaca tagtcggcga
     5281 acaatctcaa atcgacggca tccggtttca gcgtgacaat tgcttcggcc tcagccccat
     5341 agaaccgcgc agcacgctgc gtgaaattct gcacgaggaa agccccattg gggtccagca
     5401 cgccgtgatc atccgagcga tcgggtacgc cgtctccatt cgtgtcggca ctacgtacga
     5461 aaatgtagtt gtcgatccag ctatggaaaa aattgagctt ccatttgata ttgccgtcgg
     5521 tttttcgcag tgccaggtcg atgttattgg ttgtctcgct ggttaaacgg ggatcgcccg
     5581 tctggaaagt ggcggtacca cgatgtgcgc cgtgtatata cagttcttcc gccgctggcg
     5641 cacgctgtcc gcgtgtaccg gaaagggtga gtcggtaccc atccatgaac ttccatacgg
     5701 tggccgcaga aacgttataa aggttgaatg cacgggatgg gttcgtgcct tggcgcggat
     5761 cgcgtgtcga acgctcgatc cgcccgccca gttccacccg ccagcggtcc cagtttcgct
     5821 cctccacgag gaacagtcct gctgagtcgg atttcgttac tggcacgacc gcttcttcgc
     5881 caagggcgga gaaattccgg tacagaaact gaaagccgaa tacgccctgc cactttgcca
     5941 aaggcgcatg caaaagctcg gcgcggctct ccaaggcctg gttcctgaat cgggtagcaa
     6001 tcgcccccgt tccctcgatt tcgttgtgct tatagtcgtt gtaacccacc cgcaccttaa
     6061 gccgctcaaa cccccggagc ggctcgttca actcgcccgc cagatcatag cgggtttgac
     6121 cgagatcgat cctggagcgc tcagggctgg gaataccgta tttgctatcc agccgcgaaa
     6181 ccgacacacc catgaaccct ttctcaccga cgaaggatcc gccaaggtat cctccgcctg
     6241 agttaaccgc gctgttctca accaccccct ttttaccggc cggatcttcc ttgttcgcaa
     6301 acccgggaat gctgtagtca ccggttttgc gcctgaatcc tcctgcactc caggacattt
     6361 ttcccaggct gccggatacg ttcaaggccc cggttctttc ctcggttgcg gtattaccgc
     6421 gtgtttcaat attacctttg ggtgatttca tccgtttatc gggaatacgg ccatttacca
     6481 cattgaccac accccctgat gctccgctgc cataaaggag ggttgcggga cctcgaagta
     6541 tttctatttg cgaagcgttt agcgattcag cggtaaccgc gtgatccggg ctgaccgttg
     6601 aaatatcgag cgtgccgatc ccattgtcga gaactcgaat ccgtgggcca tccagccccc
     6661 ggatgatcgg gcgaccggca ccgggcccaa aggaactcga ggcgacgccc aactcgcggg
     6721 aaagtgtgtc gcccaggctt gcttcacgct tccggcgcag gtcatcgcct ttaagtaccg
     6781 aaacgggttg agccatctcg agctcactct gattgctgaa aggtgtagcg gtcacgacaa
     6841 caggctgaag tgtgttgccg ctgtcctctg cttgcgcgga cgccgtaccc gttatgagtg
     6901 caaacatgat gctgacgaac gatccataag aatgacagat ccccccagct gagcgattcc
     6961 tgtcgggcgt attcatattg cgtaaagccc acggatcctg caactgagtt gcaaaaggca
     7021 gtagttgcat aaggcagccg agttgcagga accttgtata gtcttgctat ttgttcgcga
     7081 tcaatgctgg ctgggtagcc ctgggactgg aacaggcaga atgccagtca cgggcagcca
     7141 acaaagaatg aaaagatgcg ggggttaggc ttgcggaggc gcgcgggaaa gatacggggt
     7201 ataaaaatcg aggaaatgtc cccttgaatt caggtgccac aggtgcaacc gcctgatcgc
     7261 accgaaaaaa agaaccgcca gggtaagaag ggcgacgctg aaggccgcca gcgccagaca
     7321 gtccagacaa attttatcca atccggaatc tgcatcttct ccgtcttcgc ctgctccgac
     7381 accgcctgtt tgagcgacaa gcactgcatc atggaggcta tcaggggttc tgatatgagt
     7441 cagcatatgc gccgactgga agatgggaac cagcacaagc gcgaacaaga gcagcgcgct
     7501 aaacgcgcgc aacaggagca ggagccgatg agcgctcata cgcagcctgg acataatcct
     7561 ctcaccctca actcaacctc ctgagagcga taccccgcgg gtagtggagg atcgtactcg
     7621 gccttgagat cgtcgaggca gatcacgcgt gtacagcggg tgcattcaaa gtgagcatgc
     7681 tgatgaggtt ttaaatcgaa atttgcccga aaacgccata cacgatcatc acccaccacc
     7741 ctgtggatat atccccgttc gttcagccat tcgagcacac gataaagcgt gacgcggtcc
     7801 agccgatgct tacccttgaa tcgctgctca agttcatgat ggctgacagc ccgttgctcc
     7861 tcgaggagca tggcaagaac ctggacgcgc ccccgcgtga cgcgctccgc cctgcccaga
     7921 attatctctt ccgcctgttt tgtgaagcca cgggacataa aggtagattt gcaacaaagt
     7981 tgcattattt tagctgggta ataaggagtg agcaattcga accaacatgc gtgaatagca
     8041 tgcgtgggac aaatactctt ttcaggccgc tctggcgata cctgaggcaa tcagtatgag
     8101 atgagtgcct tataatgatg cgtctgagct ttcctcgatt aatacaaggg ctttgattgc
     8161 cagcgaggca atacctgacg gccgctttta attgctacga aacgatgcct aaaatcagaa
     8221 gcaagcgtct cagaaaaaag ctgcaccttg atgagtttca ggaattcgga tttttgattt
     8281 cgttcacact gcctcaaaat cttgataacg aagcccttga tatttttttt gatcgattca
     8341 tcgacgtcat tgagcacaac ggcttattat tcggcggcgg tatcggatca aatagcgagg
     8401 gatttgtaac tctggataaa cgaggaaatg ctactgaaga gtatcgagag cttgtgagaa
     8461 agtggttagc tttgcatccc cttgtatccg gtattcaaat ctgggaactg gttgatgcgt
     8521 gggtttagag gacatttcaa gaaagaagcc gcaaagcact tgcttcggcc tttttccatt
     8581 ttgcgtcaac aattgaaagg atattcggag tacagctgag gtgcatgggc catgagaaga
     8641 tatctcgagc taactcagcc gtacgttcga ggctatccac tttttccggc gacggattgg
     8701 ccgacatacg ggacagcaag attcgtaaca agatgactaa cagtcgtttc ttgcctatca
     8761 gccgtgccgg attgcccggc ggcgccagtc gagccaagca tccattcgcg tacgatagta
     8821 gtgaagggca tcgtgctgat ggtgttccac ttcgtatttc ttgccgtgct cgatga
//
LOCUS       DASSHJ010000108         9052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1227750,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000108 DASSHJ010000000
VERSION     DASSHJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227750"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /gene="rpsP"
                     /locus_tag="VFI43_04320"
     CDS             complement(<1..114)
                     /gene="rpsP"
                     /locus_tag="VFI43_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7061384.1"
                     /translation="MVIIRLARGGAKKRPFFNMVVADSRDRRDGKFIERVGF"
     gene            854..1306
                     /locus_tag="VFI43_04325"
     CDS             854..1306
                     /locus_tag="VFI43_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7061385.1"
                     /translation="MSERINLGKSAPALYQAVIGLEKVATEAVASASIPDGFAHLLRL
                     RASQLNQCAFCVRMHTRDALSSGESSDRISVLPAWREAGYFTEKERSSLALVEAITLI
                     SDGQVPDAIYEQATANLSKDEISAVEWLSVVINAWNRIAISSRYPVKP"
     gene            1555..2376
                     /locus_tag="VFI43_04330"
     CDS             1555..2376
                     /locus_tag="VFI43_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7061386.1"
                     /translation="MNTLAIKDGTTLYYKDWGNGQPVVFSHGWPLNADAWDAQMLFLG
                     QCGFRVIAHDRRSHGRSGQTWDGNEMDTYADDLAALFEKLDLKDAVMVGHSTGGGEVA
                     RYLGRHGSKRVSKAVLISAVPPIMVKSAKNPGGLPIDAFDEIRAGMLKDRSQFYKDLS
                     LPFFGYNRAGAKISEGVRENFWFQGMQGGIKGQYDCVKAFSETDFTEDLKKIDISTLV
                     MHGDDDQIVPFPDAGALSVKLVKNAQLKVYPGFPHGMPIIHAEQINPDLLAFIQS"
     gene            2895..3314
                     /locus_tag="VFI43_04335"
     CDS             2895..3314
                     /locus_tag="VFI43_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7061387.1"
                     /translation="MMFTRFLCAVALAALSINAASADSLPTDKSKVTLVFNHVLPNVP
                     GKSMKGVLVEYQPGGSSPAHTHPKSAFIYATVLEGAIRSKVNDGPEKVYRAGESFAEL
                     PGDHHGVSANNSKTEPARLLAVFVVDTDEKNLVTNDK"
     gene            complement(3507..4979)
                     /gene="trpE"
                     /locus_tag="VFI43_04340"
     CDS             complement(3507..4979)
                     /gene="trpE"
                     /locus_tag="VFI43_04340"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET7061388.1"
                     /translation="MTEAEFEALAAQGYNRIPVVLETFADLDTPLSVYLKLANVPFSY
                     LLESVQGGERFGRYSFIGLPATTRIQVCGNRVNLITHLKRQEIDVPDPLAFIETYLSR
                     FKASPYPGLPRFCGGLVGYFGYDVVRYIERKLDGQAPFDTLGTADILLLLSEELAVVD
                     NLAGKLYLIVYADPAEDDAYSIARKRLKELLEKLREPLEIPVELPCQPGQAVTEFEES
                     DFIAAVERAKRYIFDGDIMQVVLSQRTSKPYSASPLALYRALRSLNPSPYMFYYHFGD
                     FHVVGASPEILVRLEGETVTVRPIAGTRPRGKTVQEDSTLATDLLADPKERAEHLMLM
                     DLGRNDVGRVARTGTVAVTENMQIEHYSHVMHIVSNVDGKLKAGLKAMDVLRATFPAG
                     TVSGAPKVRAMEIIDELEVSKRGIYAGAVGYLGFNGDMDLAIAIRTGIVKENRLYVQA
                     GAGIVADSVPRNEWIETQNKAKALLRAAEIAEGGLDSRVE"
     gene            complement(5117..5845)
                     /locus_tag="VFI43_04345"
     CDS             complement(5117..5845)
                     /locus_tag="VFI43_04345"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HET7061389.1"
                     /translation="MDKGVSSASASPASEVKFPLLVKAVMIDLDGTLLDTAGDLAAAA
                     NMMLRELNRPELPVEVIRSYIGKGVQRLVKRTLCGSLDGEPDPELFKRAMPVYERDYA
                     KTLCDSTRPYPGVVDGLEAFKESGFRLACVTNKAEAFTLPLLQATGLMCYFEIVLSGD
                     SLPRKKPDPLPLVHACNHFGIRTGEMLLIGDSLNDTQAARAAGSHVFCVPYGYNEGRD
                     VHELDCDAIIPSLYQASKLIRKPS"
     gene            complement(5838..6551)
                     /gene="rpe"
                     /locus_tag="VFI43_04350"
     CDS             complement(5838..6551)
                     /gene="rpe"
                     /locus_tag="VFI43_04350"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7061390.1"
                     /translation="MTKYRIAPSILSANFAKLGEEITAVIASGADFVHFDVMDNHYVP
                     NLTIGPLVCKAIRPLTDAVIDVHLMVEPVDRIVPDFAEAGANIISFHPEATRHIDRTI
                     GLIHEHGCKAGLVFNPATPLNYLDYVLDKLDMVLIMSVNPGFGGQKFIPEALPKLRSV
                     RQRIDASGKDILLEIDGGVKVDNIAEIARAGADTFVAGSAIYSAGKDSDPHRYDSIMK
                     ALRAELEKVKPTESAEIYG"
     gene            6794..7177
                     /gene="apaG"
                     /locus_tag="VFI43_04355"
     CDS             6794..7177
                     /gene="apaG"
                     /locus_tag="VFI43_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET7061391.1"
                     /translation="MTETKKYEISVKVQTTYLPEQSDETLDRYVFAYTIVISNIGTVP
                     AQLISRHWIIADGSGGVQEVRGLGVVGEQPLLKPGESFEYTSGTAISTPVGSMKGTYQ
                     MVAEDGLRFDAPIPEFILSVPRVLH"
     gene            complement(7274..8674)
                     /gene="der"
                     /locus_tag="VFI43_04360"
     CDS             complement(7274..8674)
                     /gene="der"
                     /locus_tag="VFI43_04360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7061392.1"
                     /translation="MKPTLVLVGRPNVGKSTLFNRLTRSREAIVADVPGLTRDRHYGH
                     GRLGNKPYLVVDTGGLEPEATEGILHEMAKQTRLAIDEADLVLFIVDGRSGLAPQDKI
                     IAEQLRRTGRRVMLVVNKTEGMSPSAVTAEFHELGLGEPYAISAAHGDNVNELVDLAL
                     AELPFPTENEQEDTHPKIAIVGRPNAGKSTLVNALVGEERVIAFDQPGTTRNSIYVDF
                     DRNGRHYTLIDTAGLRRRGKVIETVEKFSVIKTLQAIEDANVVVLMLDAHAEISDQDA
                     HIAGFALEAGRALVVTVNKWDGLDEYQRETIKSAIARKLSFLLSFAKVIYISALQGTG
                     MKALLPAIDAAYAAAMARLPTPKLTRALIAAVEKQPPPRAGLFRPKLRYAHQGGSNPP
                     IIVIHGSGLDRVSQTYQRYLENTFREGFQLEGTPLRVEFKKSENPYAGKQTGPLTPAQ
                     AEQAHRRRRYGRKKYG"
     gene            complement(8784..>9052)
                     /locus_tag="VFI43_04365"
     CDS             complement(8784..>9052)
                     /locus_tag="VFI43_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061393.1"
                     /translation="VADSQGYVNVIGKSDGNILGRAPTDYTAIAARPVPLDNGVAVQT
                     KKGGLFAFNTDVSALSIPGQSAGSGGSGGSAIGDYFRGFLPFSK"
BASE COUNT         2071 a         2474 c         2454 g         2053 t
ORIGIN      
        1 aaagccgacg cgttcgatga acttcccatc gcggcgatcg cgtgaatccg ccacaaccat
       61 gttaaaaaac gggcgcttct tggcgccgcc tcgcgccaat cgaataatga ccatgtacgt
      121 tacctaaggt gagaaggagc ccagaaacca aaaagggtgt gattttacgg taaattttac
      181 tggagaagca agtaaaaagt gacagttctc ccgcacgtcg cacaagcccc cgagtccggg
      241 ctcctttcag cggagatagt ttaattatta aaatcaccga cgaagaatac tggaaaggtg
      301 aattggcaag cgcaacccta taaaaacgag actacctcaa gggctagtcg ctaggccaaa
      361 gctgctagcg ttgattttga cttgcgttct cccgtggtcg atggaactct agattaccct
      421 gattataaaa tggagacgga aacctggcga cgtcaataat agaggctttg aggtactcga
      481 ggtactcgag atactgcaaa actgctctgc cttgcttttt ctttatcctc acctataaat
      541 gaaacgaata gcagaatggc attgtcggct agacgtccgt tgccagacgt ttaatggaga
      601 taatcatgag caggcggacc gcgtggagcg ggtggatcgc ctgcgatgaa cgtccgcgta
      661 tttcgcatcc cttcacacag atgagttggg cggtcgtcga tgttaaaggt tcgcgacgcc
      721 gaacccaacg ctgcacggac gcgacggcgt aatccgtttt ccgctgctcg ccggacttgc
      781 gtcatcgcac aggttgtgcg agcgggccag cgattttatt cgttagccct gtacatacag
      841 gtaaggagat ttcatgtcag aaagaatcaa cctcggtaaa tctgctccag cactttacca
      901 ggcagtcatt ggccttgaga aggtggcgac tgaggccgtc gcttccgcaa gcattccgga
      961 tggctttgcc catctgttgc gccttcgcgc atcgcaactc aatcagtgcg cattctgtgt
     1021 caggatgcat actcgcgatg ccctatccag tggcgagtcg agtgatcgca taagtgtctt
     1081 gccagcttgg cgggaagccg ggtatttcac tgaaaaggaa cgctcatcat tggcactggt
     1141 agaagcaatt acactgatct ccgacgggca agtgccagat gccatttatg agcaagcaac
     1201 agccaatctg tcaaaagatg aaatatcggc tgtggagtgg ctttcggtag tcatcaatgc
     1261 ctggaatcgc attgccattt ccagccgtta ccctgtgaag ccgtgacatg gctcccaatt
     1321 cattcaagcc gaagcgcttc tcggctccgc ttaattcaag cgttgggcat ggcgatttca
     1381 acggaacagg agagcaatgt gatgaaacag tatcggatct tggtggtagg tggttccggg
     1441 ccgtatggca gcagacgggg cgacgaactg cgcgtctgat ttggcccgtg agaaagctgg
     1501 caatcactcg cagcgaagca atagcaggac aatcaacaag aaaaggaaaa tgccatgaat
     1561 accctcgcca taaaagatgg aacaactctg tactacaagg actggggtaa tggacagcct
     1621 gttgtgttct cacatggctg gccacttaac gcggacgcct gggacgcgca gatgcttttc
     1681 ctgggacagt gcggcttccg cgtgatcgca cacgatcgcc gtagtcatgg ccgttcaggc
     1741 cagacgtggg atggcaacga gatggacacg tacgctgacg atctagctgc gttattcgag
     1801 aaactggacc tgaaagatgc agtgatggtg ggtcattcaa ccggtggcgg cgaggtcgct
     1861 cgctatctcg gtcggcacgg ctcaaagcga gtgtccaagg cggtcctgat cagtgctgtg
     1921 ccgcccatca tggtcaagtc tgccaagaat cccggcggct tgccgatcga tgccttcgat
     1981 gagattcgtg ccggtatgtt gaaggatcgc tcgcagttct acaaggatct gagtctcccc
     2041 ttctttgggt ataacagagc gggagccaaa atctccgaag gtgtgcgtga gaacttctgg
     2101 tttcaaggga tgcagggcgg catcaagggg cagtacgatt gcgtcaaggc tttttccgag
     2161 accgacttca cggaagacct gaagaaaatc gacatttcca ccctggttat gcacggtgac
     2221 gatgatcaga tcgtgccgtt cccggatgca ggagcactct ccgtaaaact ggtaaagaat
     2281 gcccagctca aggtctatcc cggttttcct catggcatgc cgatcattca cgcagaacag
     2341 atcaatcctg atctgctcgc gttcattcag tcatagcccg gaactgccgg gacgagtacg
     2401 gtttttttcc tggggacgct tcttgtaact tctatgacgc agacacatcc atctcacaga
     2461 gtcgagacaa gtacgtccct gtttcgccaa gatgggctta ttaaagaagg catgagttga
     2521 catggctccc gagtcgaagt ggcgtcgctt ctaaaaaaac caatttttcc ggttggaata
     2581 aggtgtcatt tcaacacgat tgctttagtg ggctcacgtt acttcggtgc ggagttgaac
     2641 gatcatcccc ggccacaatg cgcgtatcgg cccggcgagt tttgaggcat gactgcagtc
     2701 caatcaccaa aagtaagccc gcccggctgt gtgaacgccc ggataaagcc atgccccaag
     2761 gcgggaccat accgcgcgtt cacctgtcca tcttttccga agaataggcc ttctgatccg
     2821 cgatcagaca gatggggata aggggcatca tggggtggat ttgtatccgc gaatttttat
     2881 caacgaagga gaacatgatg tttactagat ttctctgcgc agttgcgctc gcagctctct
     2941 ccataaatgc agcttcagcc gatagcttgc ctactgataa gtccaaggta acgctggtct
     3001 ttaatcacgt tcttccgaat gtgcccggca agagcatgaa aggcgtgctg gtcgaatacc
     3061 agccaggcgg ctcatcgcca gcacataccc atccaaagtc agctttcatc tacgcaacgg
     3121 tactggaggg ggcaattcgc agcaaagtca atgacggtcc ggaaaaggtt tatcgcgccg
     3181 gcgagagctt cgccgaacta cctggcgatc atcatggtgt cagcgcaaat aacagcaaga
     3241 ctgagcccgc acgtctgctg gccgtctttg tcgtcgatac ggacgaaaaa aacctcgtca
     3301 cgaacgacaa atagtgagaa gaaaaatcgc atgttagatc gcgatgaatt caacgctatt
     3361 caatcctcgc gtaacacaaa tgcgcgaatc tggtacaggc gtttctttcc gggaaaccat
     3421 ggctggcacc cactccgtct ccatttccgc tcgcagtacg ggccggggat cgggagcaag
     3481 gtgtaattca ggcgggcagc gctttctcac tcgactctgc tatccagtcc accctcggcg
     3541 atctccgcag cccgcagcaa cgccttggcc ttgttctgcg tttcaatcca ttcgttgcgc
     3601 ggtacggaat cggcaacgat gccggcgccg gcctgcacat ataaccggtt ttccttcaca
     3661 atcccggtcc gaatcgcaat ggccagatcc atgtccccat tgaatccgag gtatccgaca
     3721 gctccggcat agataccacg cttggacacc tcgagctcat caatgatttc cattgcccgt
     3781 accttgggag cgccgctcac ggtcccggcc ggaaaggtag cgcgcagcac gtccatggcc
     3841 ttcaaaccgg ccttgagttt cccatccacg ttcgacacga tgtgcattac atgcgagtaa
     3901 tgctcgatct gcatattctc tgtaaccgcg accgtacccg tccgagccac ccgcccgacg
     3961 tcattgcgcc ccaggtccat cagcatgagg tgttccgccc gctctttcgg gtcggccagc
     4021 aagtcggttg ccaacgttga gtcttcctgt accgttttgc cgcgaggccg tgtgcccgcg
     4081 ataggacgta cagtcactgt ctcgccttca aggcgtacga ggatctccgg cgaggcccct
     4141 accacgtgaa aatcaccgaa atgataataa aacatataag gcgagggatt gaggctgcga
     4201 agcgcgcgat ataaagccag gggggacgcg ctgtaaggct tgctggtacg ttgcgagagc
     4261 acgacctgca tgatatcgcc gtcaaaaatg tagcgcttcg cccgttcaac tgccgcgatg
     4321 aaatcacttt cctcgaattc ggtaacggcc tgtcccggct ggcagggcag ttcaaccgga
     4381 atttcgagag gctcgcgcag cttctccagc aattctttca ggcgttttct agcgatcgaa
     4441 taggcgtcat cctccgctgg gtcggcgtag acgataaggt aaagcttgcc agccaggtta
     4501 tcgacgacag ccagctcttc cgatagcaat aacagaatgt ccgccgtgcc gagcgtatcg
     4561 aagggcgctt ggccgtcgag ctttctctcg atataccgga ccacatcgta accgaaataa
     4621 cccaccaatc ccccgcagaa gcgcggcagc cccggatacg gcgacgcttt gaaacgcgac
     4681 agataggttt cgatgaaagc gagcgggtcg ggtacgtcaa tttcctggcg cttcaaatga
     4741 gtgataaggt tgacgcgatt accgcacact tgaatgcgtg ttgtggcagg caggccaata
     4801 aacgagtagc gcccaaaacg ctcgcctccc tggacggatt caagcaaata agaaaaagga
     4861 acgttggcga gcttgagata aacggatagc ggcgtgtcga gatcggcaaa cgtttcaagc
     4921 actacgggga ttcggttgta gccctgggcc gcaagggcct cgaattcggc ttcagtcatg
     4981 actttctcca ggaaaatgga aataaaaaat acggcagcag cttctcagcc atgccatcga
     5041 tcagcggccg gccaggaatg ccggaccttg atcgtgtatt ttttgatctc tgattttctt
     5101 gaagtcatga cggatgtcac gagggtttgc ggatcaactt gcttgcttga tagagagagg
     5161 gaatgattgc gtcgcaatcc agctcatgca catccctgcc ttcgttgtac ccgtaaggga
     5221 cgcagaagac atgcgatcca gcggcccgcg ccgcctgggt atcgttcagc gagtctccga
     5281 tcaatagcat ttcgccggtt cgaatcccga agtggttgca tgcatgcacc aacggcaaag
     5341 gatcaggctt ctttcggggc aagctatcgc cggataacac aatttcgaaa taacacatca
     5401 ggccagtggc ttgaagcaag ggcaacgtga aagcttccgc tttgtttgtc acgcaggcaa
     5461 gccgaaatcc gctttcctta aacgcttcga gcccatctac aacgccggga tagggacgcg
     5521 tgctgtcgca aagggttttc gcgtaatccc gctcgtatac cggcatcgcg cgcttgaaca
     5581 gctcgggatc gggttcgccg tcaaggctgc cgcacaaggt ccgcttcacc agcctctgga
     5641 cacctttacc gatgtacgac cggataactt ccacaggcag ttccggtctg ttcaactccc
     5701 gcagcatcat gttggctgcg gccgcgagat ccccagccgt atcgagaagc gtgccgtcca
     5761 ggtcgatcat gacggctttt acaagcaaag ggaattttac ttcggacgcg ggacttgcgc
     5821 tcgccgacga gacaccctta tccataaatt tcggctgact cggtcggttt taccttctcc
     5881 agctccgccc gcagcgcctt cataatgctg tcatatcgat gaggatcgct gtccttcccc
     5941 gcgctataaa tcgcggagcc cgcgacgaaa gtatctgcgc ctgcccgggc gatttcagcg
     6001 atgttgtcga ccttcactcc tccatcgatt tcaagcagga tgtccttgcc tgacgcgtcg
     6061 atccgctggc gtacgctgcg gagcttgggt agcgcttccg ggatgaactt ctgcccgccg
     6121 aatcccgggt tcaccgacat gatgagcacc atgtccagct tatcgagcac gtaatcaaga
     6181 taattcaacg gcgttgcggg gttgaatacc aggccggcct tgcatccatg ctcatgaatg
     6241 aggccaatcg tacgatcgat gtggcgagtc gcttcgggat ggaaggaaat gatgttggcc
     6301 cccgcctctg cgaaatccgg aacgatacgg tccaccggtt cgaccatcag atgcacgtcg
     6361 atgacggcgt cggtaagcgg gcggattgct ttacatacca gcggcccgat ggtgagattg
     6421 ggaacataat gattatccat cacgtcgaaa tggacaaaat ctgcgcccga ggcgatcaca
     6481 gcagtaattt cttcgcccag cttcgcaaaa tttgcggaaa gaatgctggg agcgatgcgg
     6541 tacttggtca tcaagacttc cttattgtct ggaattattg aaaaatggca attctaacct
     6601 aaatcgggcc gcaaactgtc aaagagaatt cggaatccct taggaagcgg gggaggcaac
     6661 tgatctgagt ggtcagaccg gcgagtgtga ttaagttgct tgcctgtcca attcaacgga
     6721 cgcttctctc gtactctgca ttttcgtgat caccgcactt gccgttccag gaaaattagt
     6781 gtgcaattcg gttatgactg aaacaaaaaa atatgaaatc tctgtaaagg tccagacgac
     6841 ttatcttccc gagcagtcgg atgagacgct cgaccgctac gtatttgcgt atacaatcgt
     6901 aatcagcaat ataggcacgg ttccggcaca gctcatcagc cggcactgga ttatcgccga
     6961 tggaagcggt ggcgtgcagg aggtacgggg attgggcgtc gtaggagaac agcctctgct
     7021 caagcctggg gagagtttcg aatataccag cgggacggct atctcgacac ctgttggatc
     7081 aatgaaggga acgtaccaga tggtagcgga agacggactg cgttttgacg cacctatacc
     7141 tgaatttatt ttgagcgtcc ccagggtttt gcactagatc tgcccattcc cccgcgtgct
     7201 cgactataca ttccgctaac tgcgatccgg cccataggtg ttgccgtgca gcagcagaga
     7261 catgcgatat cagctaacca tattttttac ggccgtacct gcggcggcgg tgcgcctgct
     7321 cggcttgagc tggggtaagc ggccccgtct gcttcccggc ataaggattt tcgcttttct
     7381 tgaactcgac cctcaggggc gtcccctcga gctggaaacc ttcccggaag gtgttttcga
     7441 gataacgctg gtatgtctgc gatacgcgat cgagcccgct gccgtgaatc acgattatgg
     7501 gagggtttga gcctccttga tgcgcatagc gcagcttggg gcgaaatagc ccggctcgcg
     7561 gcgggggctg cttctctaca gccgcgatga gcgcccgggt aagcttgggc gtgggcagac
     7621 gtgccatggc ggcggcgtag gcagcgtcta tcgcgggtag cagtgctttc atacccgtgc
     7681 cttgcaaggc tgaaatatag atcaccttgg caaaactcag cagaaaagac agctttcgag
     7741 caatcgcgct cttgattgtc tcccgttggt attcgtccaa tccgtcccat ttgttgacgg
     7801 tgacaaccag cgcacgtccc gcctccagcg caaatccggc aatgtgagca tcctggtccg
     7861 aaatctccgc gtgtgcatcc agcatcagca ccaccacatt cgcgtcctct atcgcctgga
     7921 gggtcttgat taccgaaaat ttctcgactg tctcgatgac cttgccgcgg cggcgcagcc
     7981 ccgccgtatc gatgagggtg taatgacggc cgtttcggtc gaagtccacg taaatactgt
     8041 tgcgcgttgt ccccggctga tcgaaggcaa tgacgcgctc ctcccccacg agggcattca
     8101 cgagcgttga ttttcccgca ttgggccggc ctacgattgc gattttgggg tgagtgtctt
     8161 cctgctcatt ttcggtagga aagggaagtt cggctaatgc cagatcgaca agttcattca
     8221 cgttgtcgcc gtgcgctgct gaaatggcgt aaggctcgcc caagcccagt tcatgaaact
     8281 cggcagtgac tgcagaagga gacattccct cggttttatt caccaccagc atgaccctgc
     8341 gccctgtcct gcgaagttgc tcggcgataa ttttgtcctg aggcgccaag ccgctcctgc
     8401 cgtcgacaat aaacagcacg aggtcagcct catcgatcgc caatcgcgtc tgcttcgcca
     8461 tctcgtgcag aatcccctcg gtggcctcgg gctccaggcc gccggtatcc acgacgaggt
     8521 aaggtttatt ccccagcctg ccgtgaccat agtgccggtc ccgcgtcaag cccggaacgt
     8581 cggccacgat agcttcccgg ctgcgcgtca gcctgttgaa aagcgtggac ttgccgacat
     8641 ttggccttcc gacgagtacg agagtaggtt tcatgatttt tttgcggccc taatgaagaa
     8701 acccgttctt ggtttccccc ggcaatttct ccaaaaactg gcttctctcc gtttttaagg
     8761 aggtcaatgc gcggagtcgc gcattacttg gaaaacggaa gaaatccgcg gaaataatcc
     8821 ccgatggctg atccgcctga tccgcctgat ccggctgatt gaccaggtat ggataaggcg
     8881 ctgacgtcag tgttgaatgc aaataatccg ccttttttcg tctgaactgc cacaccgttg
     8941 tcgaggggca caggacgcgc ggcaattgca gtgtaatcgg ttggcgcgcg cccaagaata
     9001 ttgccgtcgc tcttgccgat gacattcacg tatccctgcg aatccgcgac ga
//
LOCUS       DASSHJ010000109         7943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_33632, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000109 DASSHJ010000000
VERSION     DASSHJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33632"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="metG"
                     /locus_tag="VFI43_04370"
     CDS             <1..428
                     /gene="metG"
                     /locus_tag="VFI43_04370"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HET7061394.1"
                     /translation="GQSPQTGQTQAMDKNSSDDSNSKGSEVAGASVPSVIGIDDFAKV
                     DLRVAKIIDAERVEGADKLLKLTLDIGSEQRTVFAGIKASYAPEQLKGRLTVMVANLA
                     PRKMKFGLSEGMVLAAGGDDGGPYLLAPDEGARPGMKIK"
     gene            1105..1953
                     /locus_tag="VFI43_04375"
     CDS             1105..1953
                     /locus_tag="VFI43_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HET7061395.1"
                     /translation="MPHPLSLQHFILATAVIFSLASAHTTGAVTAMDTAKIEQITGLK
                     GKFLQEENVFKVSKPRTDVKIQIDKWVMTPFMGLTSWAAFTPAENGQVMMMGDTVLFE
                     DEVNPAISIALDAGLEVTALHNHFFFEQPKVYFMHIDGVGTIAQLATGIKRIYDKVIE
                     IRAANPTPGADFSKTIAEENKITTGPLETILGMKGEANNGMFKVTIGRDATIHDVKVG
                     KQMGVNTWAAFAGSDAQAVVDGDFVVLEDELQPALKTLRSGGINIVAIHQHMTHEQPR
                     YLFIHY"
     gene            complement(2079..3406)
                     /gene="chrA"
                     /locus_tag="VFI43_04380"
                     /pseudo
     CDS             complement(2079..3406)
                     /gene="chrA"
                     /locus_tag="VFI43_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
     gene            3669..6215
                     /locus_tag="VFI43_04385"
     CDS             3669..6215
                     /locus_tag="VFI43_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HET7061396.1"
                     /translation="MTTSKPQTTDFTKDVLGRYINNGLDEALNSTNGNGQRPDGSPTS
                     DAQQFDVIVIGGGSFGPVFAQHLFSNDHTHSHRILVLDAGSLLLTEHVQNYPPIGLGV
                     PPPTESDPFQLRNEVWGLPWRSDVPQGFPGLAYCTGGRSLYFGGWSPQLLDTPTDTEM
                     PRDRWPDSVVKDLNNKYFSQAAQQIGTDQTNDFISGEMHDALRKQLFEGIKANKVPDA
                     IKLSKLPLHLDLPPGIPAALKEQFKLEAPLTVKSRAGSGLFPFNKFSSMPLLIAASRA
                     ASTESTHAVGYPDDIKKRLMVVPHCHVIRLVTNIQNGIGRVTGVECETSLPICGDSSN
                     VQKQRITIPVSDNANVVIALGTIESARLALLSFQGIKNYDKIGTNLMAHLRSNITISI
                     PRTSLSSLDPTVKALQASALFVKGSHSFKDGSGKGYFHLQITAAGLDKLTSDSEAELF
                     KKIPDLDNMLPLQEVNDNTIVITIRGIGEIQGQNPGNKITLSGETDEVGVQRAFVTYN
                     LGEKDFELWDAMDKASDDVARVFAGGHNFTVFKAPDRPVTVSPTDDLSQVLPYTPVWQ
                     GGRRDGMGTTHHEAGPLWMGVDSNTSVTNTDTRFHFVENAYVAGPALFPTVGSPNPML
                     TGVALARRLADHLTVGPFKADAGFTLLFDGADTSKWRLTTIQNQANNFPGGRLVVDRA
                     LETVPGNDLGMFWHTDPTPHDFVLKLEWLRWREDDNSGIFIRFPHPDSKGYNNTAYVA
                     VNFGFEIQIDQLARPDGSPLRKTGAIYGFAAPSDPNNLPVKPVGEWNEFQIHVQGQHY
                     TVFLNGVKITEYNNTDPNRGQPSTGSNPSFIGIQNHTGRVAFRKIQIKAL"
     gene            6271..7437
                     /locus_tag="VFI43_04390"
     CDS             6271..7437
                     /locus_tag="VFI43_04390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7061397.1"
                     /translation="MRILHLTSEYPPTVWGGLGTVVGGLTVASVRAGIEVDVLLVRHE
                     GQLSYGNLAPAWTVAVPGEEKEASGDEFPINIFQIPHAGAAAAAIRLARDRRPDIIHI
                     HPVELWPVAKAIKQATGIPIVYTVHSLNLAEYEFGNEPPEILNLWQTQQELIAGADRV
                     LVLTQDEKSLLLECCPSVLDRVRVVGNGINNSAAATRSASKKKQNDPPLVLYSGRFVD
                     RKGIHELFRAIPVVLQSAPETKFVFVGGYGKGEDIERAWLPAELLCYREQIHFTGWLS
                     SIEVGKWYERANILVVPSWYEPFGMVVLEGMLYGLSVAASNIGGPAEILEHENTGLLF
                     LPKDVENLASALLNLVANSDLRERLGANAAIEIRKKWLWPRIVDQIKEIYQQVL"
     gene            7672..>7943
                     /locus_tag="VFI43_04395"
     CDS             7672..>7943
                     /locus_tag="VFI43_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061398.1"
                     /translation="MKCNRQTILAVLMLAVLTIQVAWVSNTPTATASDSDKIGKPVTH
                     LDRQIDKHAQRTVAEGRHIFRFDTFGDEAFWGDTIKLHQAIQGKRFG"
BASE COUNT         2139 a         2009 c         1986 g         1809 t
ORIGIN      
        1 gcggccagtc gccgcaaacc gggcaaacgc aagcgatgga taaaaactcc tcggatgatt
       61 ccaattcaaa aggctctgaa gtcgcgggag cgtctgtacc ttccgtcatc ggaatcgacg
      121 attttgccaa ggtcgatctg agggtggcaa aaatcatcga tgccgagcga gtggaaggcg
      181 ctgacaagct tcttaaactc acgctcgata tcggaagcga gcagcgaacc gtctttgcag
      241 gaataaaggc gagctacgcc cctgaacagc tcaagggacg gcttacagtg atggtagcca
      301 acctcgcacc ccgcaagatg aaattcggcc tgtcggaagg aatggtcctc gccgcggggg
      361 gcgacgacgg agggccttat ttgctggccc cggatgaggg agcacgtccg ggaatgaaaa
      421 tcaaataggt gttccccagc aggtcctagc aaacaaatcc ccctactacc gttccatcgt
      481 tcgaaactcg gttccatcta gggggatgta gaatatttcc tttgtgattc ttctgactcg
      541 gtgagaaatt ctccagctca tccgtctttt gcgtgaaccc aacctgcact tgatcctata
      601 accctccgca ccaaggatag cctagccgcc aatcattcaa gcggcaacaa ctagcaatat
      661 ttgggaaata tttgccagta ccagcaacaa ggctatcaat tgaaatatca agtgtagaaa
      721 attactccac ctgcctgccc catggctcat aaactccctt tatcaaatca aagatctctt
      781 gagccagttt gactttggcc gggaacgaag aatgctttta aaatgacaaa tgcaatttac
      841 ttaaatcatt tattaatggg aggaaagccg ttttcgcatc gaatacacgc tccccaattc
      901 gagtttttgc cctcaagcaa atgcaatgca ttttgatttc ctgaagaaaa atccctctat
      961 gcctggagaa aaccctgcct ataatgatat gaatgtagct gccaaactat tggataaaaa
     1021 ggattaactc tcgcttcgcc gcgacttttg ccaagtccac tcctggccgg cttttttcca
     1081 tacttttcga cgaggaggta ctaaatgccg catcctcttt ccctacagca tttcattctg
     1141 gcaactgcgg tgatcttcag tttggcaagc gcacatacga ctggcgcagt aactgctatg
     1201 gatactgcaa aaattgaaca aatcacaggc ctgaaaggta aattccttca agaagaaaat
     1261 gtcttcaaag tctccaagcc gcggaccgat gtcaaaatcc agatcgataa gtgggtaatg
     1321 actcctttca tgggattgac atcctgggcg gcttttaccc ctgccgaaaa tgggcaggtc
     1381 atgatgatgg gggatacggt gctgttcgag gatgaagtaa acccggcgat aagcatcgcc
     1441 ctggatgcag gcctggaagt tacggcattg cacaaccact ttttctttga acagccgaaa
     1501 gtatatttta tgcacataga cggcgtgggc accatagcac agctcgctac cggcatcaaa
     1561 agaatttatg acaaggtaat tgaaattagg gcagcaaatc ccacccctgg ggcagatttt
     1621 tccaaaacta ttgccgaaga aaacaagatt accacagggc cgctagaaac aattcttggc
     1681 atgaaaggag aagcaaataa cggcatgttt aaagtcacga tagggcgtga cgctaccata
     1741 catgacgtaa aggtcggtaa gcaaatgggg gtgaatacct gggccgcttt tgctggctct
     1801 gatgcccagg ctgtggttga tggtgatttt gtggtactgg aagacgaact gcagcctgct
     1861 ctaaaaaccc tccggtcagg cggtataaac attgtcgcga tccaccagca tatgacgcat
     1921 gagcagcccc gttacttgtt tatacattat tgaggcaagg gcggcgcgga ggatttggca
     1981 aaaaccgtca aaaaggcctt agaccttcaa gtcaattccc gacaatagga tttactgaat
     2041 agggaacagg tgcaaaagcg caacgctctt ctcgacgctc agtgaagcag gttcgatgcc
     2101 atgcccaaga ggccgcaact cagaataacg gggacaactc ctactttgaa acgaaataaa
     2161 gcaacgagtg ccgcgactgc cagcacgata tagatagcca gtcaacgggc tggcgtaagc
     2221 cggatggcca gaacgcatgg taggcgaaaa acagggcaag gttcacaata actccaacca
     2281 ccgcggcagt aatggcggtg agtgacgccg tgagcttcag attgccgtgt gtggtctcga
     2341 caaaggggct gagcaaaata aaaataaagc tcggtagaaa agtaaaaaaa gtagctattg
     2401 cagaagcaac aaaagccgat gcaaatatcg cctccggacc aaacacttct ttgacccaac
     2461 cgccaacgaa accaacaaaa gtcacgatca tgatgagtgg gccaggagtg gtttcaccta
     2521 gagcgagtcc atcaatcatc tggattgggg tcagccattg atagtgttcc accacccctt
     2581 ggtaaacata cggtagcact gcgtaggccc cgccgaaggt aagcaatgct gcttttgtga
     2641 agaaccagcc tacttgcgtg aaggtattat cccatccgta gaagaacctc aaggcaccga
     2701 tcgcgagtat ccaaattgca agcccgacag tcgcgacgca agccagcttt gcccaactaa
     2761 acaaggcatg ggcgggcgta ggcgtgttgt cgtcaatgag agcgcgccca tattcttttt
     2821 gaccactcgc gtgcgcggca cccgagttga agtattgagg aaacatgttt ccccctacaa
     2881 atccaatcaa accggcacac agtacgatca cgggaaacgg caatttaaaa atgaagatcg
     2941 caaagaaggc ggcgacggca atgctccaga ctatcgcatt tcgtaatact cgcgatccaa
     3001 tacgataagc agcgaacagg acgatagcga ccaccgcggg tttaaagccg tatagcatgc
     3061 ctgccacggc tggcagcgag ccataaacca tgtaaatcca ccccagagcg acgagaataa
     3121 aataggaagg caggatgaat aagctgcctg ctatgacgcc accccacgtg cgatgcagga
     3181 gccagccgtt ataggtagcg agctgctgag cttcaggacc aggaaggatc atgcagtaat
     3241 tcaaggcatg caagaaacgc ttttctgaaa tccagcgttt cttctccacc agatcgtgat
     3301 gcatgattgc gatctgtccg gcggccccgc cgaaactgat aaacccgagc ttcagccaat
     3361 aaagggccgc ttgcgccagt gaaatggacc tgggtgacgc ggggatttgt cgagccttgc
     3421 tacagggatt acccaaaatt taaccaaaat ttaaccaaaa tttaaccttt tttttacgac
     3481 ttctcctccc gatttacata tcgtacaaac caaggattca aattcatcct ggaaactgtt
     3541 caggtacgac tataaataag caagcccaag ctaacagagc agtaggaatg aggcccatcg
     3601 cgcgttatcg cctgctatgc aaaacgccgc caccttaata cccatattag aaaccaggag
     3661 attacgatat gactaccagt aaacctcaga cgactgattt tacgaaagat gtgctgggcc
     3721 gctacatcaa taatggactg gacgaagcgc tcaacagcac caacgggaat ggtcaacgac
     3781 cggacggctc tcccaccagc gatgcgcagc aatttgatgt aatcgtgatc ggtggaggta
     3841 gtttcggtcc cgtgtttgca cagcacctgt tttcaaacga tcatactcat agtcatcgaa
     3901 tcctggtact ggatgccggc tcgttgttgc taactgaaca tgtgcaaaat taccccccga
     3961 ttgggctcgg ggtaccaccc cctaccgaaa gcgacccctt ccagctgcgc aatgaagtgt
     4021 ggggtttgcc ttggcgcagt gacgtaccgc agggctttcc tggccttgcc tactgcactg
     4081 gaggacgttc gctctatttt ggcggctggt ctccccagtt actcgatact cctacggata
     4141 cggaaatgcc gcgcgaccgg tggcccgaca gcgtggttaa ggatttgaac aacaagtact
     4201 tttcgcaagc cgcccagcag attggaacgg atcaaacgaa tgattttata agcggcgaga
     4261 tgcatgatgc gctacgcaag caattgtttg agggaatcaa ggctaataaa gtccctgacg
     4321 ccatcaaact atccaaactc cctttacacc tcgacttgcc acccggcatc cctgccgcct
     4381 tgaaagagca attcaagctg gaagcaccac tgacggttaa aagccgcgcg ggctccggat
     4441 tgtttccttt caacaagttc agttcgatgc cactgctgat tgcagcgtcg cgggcagctt
     4501 ccaccgagtc aacacacgcc gtggggtacc ccgatgacat caagaagcga ttgatggtcg
     4561 tgccacattg ccacgtgatc cggcttgtta ccaatataca gaatgggatt ggacgggtga
     4621 ccggggtgga gtgtgaaaca agcctcccca tctgtgggga tagttcgaat gtgcaaaagc
     4681 aacgaataac gatccccgtc tccgataacg ctaacgtcgt tatcgcattg ggaacaattg
     4741 aaagcgctcg gcttgccctg ctctccttcc agggcatcaa gaattacgac aaaatcggca
     4801 caaatctgat ggcgcacctg cgctccaaca ttaccatcag tattccgcgt acctcgttgt
     4861 ccagtctaga tccaaccgta aaagcgctgc aggcatcagc cttgttcgta aagggcagcc
     4921 attcttttaa ggatggatcg ggcaagggat attttcacct gcagatcact gctgccggtc
     4981 tcgataagct tacctcagac tccgaggcgg agctcttcaa aaaaattcca gacctggaca
     5041 acatgcttcc cctgcaggaa gtgaacgata acaccattgt cattaccatt cgcggcattg
     5101 gcgaaattca gggacagaat ccgggcaaca agatcacatt gagcggcgaa accgatgaag
     5161 tcggcgtgca gcgtgccttc gtgacctaca atctcggcga aaaagatttc gaactttggg
     5221 atgcgatgga caaggcctcc gacgacgtcg cgagagtgtt cgctggcgga cacaatttca
     5281 cggtcttcaa agcccctgat cgccctgtaa ccgtttcgcc cacggacgac ttgagccaag
     5341 tcctaccata tacacccgtc tggcaaggcg ggcgtcgcga cgggatgggc actacgcatc
     5401 acgaggcggg gccgctgtgg atgggtgtgg attcgaatac ttccgtaaca aataccgata
     5461 cccgcttcca ctttgtcgaa aatgcttatg ttgcaggccc tgccctgttt cccaccgtcg
     5521 gatcacccaa tcccatgctt acgggtgtcg cgttggcacg ccgtctggcg gatcatctta
     5581 cggtcgggcc atttaaagcc gacgctggat ttacgctgct tttcgatggc gcagatacga
     5641 gtaaatggcg tttaactacc atccagaacc aggccaacaa ttttccaggc ggacggcttg
     5701 ttgtagatcg cgctttggag acagtgcccg gcaacgactt gggcatgttc tggcatactg
     5761 accccactcc tcatgatttc gtgcttaaac tggaatggtt gcgctggcgt gaggatgata
     5821 attccgggat attcattcgt tttccacacc ctgacagtaa aggttacaac aacacggcct
     5881 atgtcgcggt taattttggt tttgaaatcc agatcgacca gctcgcccgt cccgatggct
     5941 cgccgcttcg aaagactggc gcgatctatg gctttgccgc acctagcgac ccaaataacc
     6001 ttcctgtaaa acccgtgggc gaatggaacg aatttcagat tcatgtccag gggcagcatt
     6061 acacagtctt cctcaatggg gtaaaaatca cggagtacaa caacactgac ccaaaccgcg
     6121 ggcagccaag cacgggaagc aaccccagtt ttatcggaat ccagaaccac actggacgag
     6181 tagcgttccg caaaatccag atcaaggcgc tttgacaccc gggcggcatg tttttcgaag
     6241 tctacgccag ccagaagggt tagagggggt atgcgtattc ttcaccttac ctccgaatat
     6301 ccccctacgg tatggggcgg actcggtacg gttgttggcg ggctgaccgt agcatcggtg
     6361 cgagctggta ttgaagtaga tgtgctacta gtgcgtcatg aagggcagtt gtcctatggc
     6421 aacttggctc cggcttggac ggtagcggta cctggggaag agaaagaagc ctcgggtgat
     6481 gagttcccta ttaacatttt tcaaattccc cacgctggcg ccgccgcagc agcaatcagg
     6541 ctcgctcgcg atcgcaggcc ggacattatt catattcacc cggttgagct atggccagtt
     6601 gcaaaggcaa tcaagcaagc aaccggtatc ccgatagtct ataccgttca ctctcttaat
     6661 ctagctgaat atgaattcgg caatgaacct cctgagatcc tgaacctttg gcaaacgcaa
     6721 caggagctga ttgcaggggc agatcgtgtt ctggttctga cccaagatga aaaatctctg
     6781 ctgctggaat gttgtccatc ggtacttgat cgtgttcgag tagtcggtaa tggcattaat
     6841 aactctgcag cagcaacacg ttccgctagt aaaaaaaagc aaaacgaccc ccctctggtg
     6901 ctttacagcg gacgattcgt cgaccgtaaa ggaatccacg aattattccg agcaatccct
     6961 gtggtattgc aaagcgcacc ggaaacaaaa tttgtattcg tagggggtta tggtaagggc
     7021 gaagacattg agcgcgcttg gttacccgca gagcttttgt gttatagaga gcagattcat
     7081 tttactggct ggcttagctc catagaagtt gggaaatggt atgagcgagc aaatattctt
     7141 gttgtgccga gttggtacga gcccttcggt atggttgtgc tggagggaat gttgtatgga
     7201 ctttcggtgg cggcctcgaa cattggcggc cctgctgaaa ttctggaaca tgaaaacaca
     7261 ggtctgcttt ttttaccaaa ggatgttgag aaccttgcaa gcgcactgct taacctggtg
     7321 gcgaactccg acctgcgtga acgtctcgga gctaatgctg caatcgagat acgtaagaaa
     7381 tggttgtggc cgaggatcgt tgatcaaata aaggaaattt atcagcaagt gctttgagta
     7441 tttgatccga ggttttttag aaatagatct atctctcaga atacctgtcg acgccattta
     7501 atcaggcgca attcgattga tttaaatatc ttgctatttg gaattcatgt tcttataact
     7561 atgaaaataa agcactattg cttataagaa aaccggtttc gctttatgtg aggcggaaac
     7621 gctgccaagg cctacttaat aaaaaaatca aactcaggaa cggaggttgg aatgaaatgc
     7681 aatcggcaaa caatcttggc ggtgttgatg ctggctgtgt tgactattca ggtagcctgg
     7741 gtttccaaca cgcctacggc aactgcttcg gattctgaca agatcggtaa acccgtcaca
     7801 caccttgata ggcaaatcga caagcacgca cagcggacag tagctgaagg aagacatatc
     7861 ttccgtttcg acacctttgg cgatgaggct ttctggggcg acacaatcaa gttgcaccaa
     7921 gccatccagg gaaaacgctt tgg
//
LOCUS       DASSHJ010000110         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_782459, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000110 DASSHJ010000000
VERSION     DASSHJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782459"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..1196
                     /locus_tag="VFI43_04400"
     CDS             375..1196
                     /locus_tag="VFI43_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061399.1"
                     /translation="MALTDNCDLYGSVNENGVNGIIRHIMRQRPSLFNYATADIANNR
                     RLWCSRVDFTPDVPKNGNPFFTIMPPLPLLGTSSPQVGVGFCVQLTQARIDFFPGNTI
                     SLPAELSPPLKDQRFSLSFRFCAAIACPSQDEIDRIPFAEPNADTGAARVLPPPIVLR
                     GRLKCFCLEAFVVGHFEHALIGGHESLLGKVEDLDIVDIKPDDLEDNIVCFLKTNLNV
                     VLRQKLAVAIETFMLSFPLFGLATITLSPTPNPPVPNNPAIEDDQLKVFVTMTVI"
     gene            1209..2120
                     /locus_tag="VFI43_04405"
     CDS             1209..2120
                     /locus_tag="VFI43_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061400.1"
                     /translation="MSDITIAASANAFTRLFDTVRDNFSFSKSDAADFGPFSANYSVA
                     MHLQGGTLQLNDDNTIEVKDVDIVFDTLKVEVCFNLPGFCVGGFCIIPDPWNGCLVSF
                     PGFCIGGPVCAPIDLSGLVSEISDLKASLKPAYFVDPARLPGWSDLDAEFAGHSNKWR
                     IFIDPVWVHVDPIDIPATVGNLLENIVRDAINALIPGWVPDWAKDLLWAIIGPILDLV
                     KGLLGIAGEINDWLSDLLGNRFDLLGAIETAVADYFASQYPIYEFEDPYPILPAASGL
                     IPVKIPLRDLDAKVDSKEMTVIANIGA"
     gene            2123..2848
                     /locus_tag="VFI43_04410"
     CDS             2123..2848
                     /locus_tag="VFI43_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061401.1"
                     /translation="MKMNFDPNFFSQVGLDSGALTMLGAVVHSFCEPGEYRGVARRGA
                     EPEAVFYISVDKNSPVAQANIDLAALVQEEPAGESENGCCPGGQGNYFVVNPKGYAVF
                     HVSSGAGGYSVHVRKNDEDPKTKIFDSRELDAGDIFSAIIIRPGTYSVTNLVTGSKSE
                     VIVSYPKPGKTPYRPPAPIAVDCSEREMTLGRIELQPGQGLNFHLKTRSRIKIELVKP
                     DDGPGKSREPERTGWIKRTLAHE"
     gene            2881..3975
                     /locus_tag="VFI43_04415"
     CDS             2881..3975
                     /locus_tag="VFI43_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061402.1"
                     /translation="MSAISIGERPLPPTLVYGYIEGQLSRLVSRFGLEDQRAAILGTY
                     QLICVESLAIPLAQRPPSFSRINEDGTPFQFSLALGASKSPLQFLSEAGVAGDSAAAR
                     RRANSEALHWIATLLDAQEALLEVTPLIDEMVPGNNPDLLADSSGAIWIGAAFVQEEA
                     PKLKIYLNAKWGREADQWKRLHSFASHFNAASRWGDAKPALKREMQPLGLSITISGGQ
                     LPSGRIYLSAYGKPMAYYEALAEASAGRAFLERFRLYTDILLGENRQYPTPSTVCSFG
                     FDACGSYDFKFEMCGLRIFESDADAMARCVKWLKLQNIEPNPYLHVLQELSGGMFCSS
                     KPRLHCYLGLGAKRESIYSTFYFNPGIWNA"
     gene            4164..>4481
                     /locus_tag="VFI43_04420"
     CDS             4164..>4481
                     /locus_tag="VFI43_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061403.1"
                     /translation="MARKRAARWLLLNQLPGYGWGYGDTVGADADSTAHAILFLSAEG
                     TRLSEDSYRRLEVFQSEDGGFSTYLRGDSHGSWCVSHPDVTPMALLALLTRHVEGSVA
                     INRG"
BASE COUNT         1019 a         1151 c         1196 g         1115 t
ORIGIN      
        1 tcaaggcccc tggcggttgt cgccattccg attcccgcag cgctaatttc gcgtaggtta
       61 tgggtagaac ctctcaatca gccattttat atttcagaga gcttaccttg gttatgtccg
      121 gatgccatga cacagccgga actccaggac ctggcatggc tacctgcata aattcaattg
      181 aaatcaattt ttcctgaaat tctgcgtccg caccgggttt ttcttgattt cccgtattta
      241 ataactatgc tgatgttgtg gttatcagca aaattcgctt atcagatgta atcaatggga
      301 agcgggaagc ggtatttaat caagcaagcc gataaagaca acaaatattc tgactatctg
      361 agcgaggaag tgaaatggcg cttactgaca attgcgatct ctatggatcg gtgaacgaaa
      421 acggcgttaa tggaatcatt cgccatatca tgcggcagcg tccatccctt ttcaattacg
      481 caacagcgga tattgcaaac aaccggaggt tgtggtgttc gcgagtcgat tttacgcccg
      541 atgttcccaa aaacgggaat ccatttttta ccattatgcc accgttaccc ttgctgggaa
      601 caagttctcc ccaagtcggc gtgggctttt gcgtccagtt gactcaagcg aggattgatt
      661 tttttccagg caatacgatt tcattaccag cagagctctc gcccccgctg aaggaccaac
      721 gcttctcgct gtcgttccgc ttctgcgcgg ctattgcctg cccttcacag gatgaaatcg
      781 atcgaattcc tttcgcggag cccaacgctg atactggcgc cgcccgtgtt ctccctccac
      841 ctatcgtgtt gcgcgggcga ctcaaatgct tttgccttga agcttttgtc gtcgggcact
      901 tcgagcatgc ccttattggg ggccatgaat cattgcttgg caaggttgaa gatctggata
      961 tcgtcgatat caagccagat gatctcgaag acaatatcgt ttgcttcctg aaaacgaatt
     1021 tgaatgtagt cttgcgccaa aagcttgccg tcgccattga aacttttatg ctcagcttcc
     1081 cattgtttgg gctcgccacg attactctct cgcccacgcc gaacccgcca gttccaaata
     1141 atcctgcaat agaagacgac cagctcaagg tctttgtcac catgaccgtg atctaaggag
     1201 atagcataat gagtgatata acaattgctg cttccgcaaa cgcttttacc cggctttttg
     1261 atacggttcg cgataatttc agcttcagca agtcggacgc agccgacttt gggccattct
     1321 ctgcaaacta ttcagttgcg atgcacctcc aggggggcac gcttcaactg aacgatgaca
     1381 acaccatcga agtgaaggat gtagacattg tcttcgatac actgaaggta gaggtctgct
     1441 tcaatcttcc gggtttttgc gtcggtggct tttgcataat tcccgatccc tggaatggct
     1501 gcctggtttc gtttcccgga ttctgcattg gcgggccggt atgcgctcca atcgatttga
     1561 gtgggcttgt ttcagaaata tccgacctca aggccagcct caagcccgcc tattttgtgg
     1621 acccggcccg gcttccgggc tggtctgatc ttgatgcgga gttcgcggga cattccaaca
     1681 agtggaggat cttcatcgat cccgtctggg tgcacgtcga cccaatcgat attcctgcaa
     1741 ccgtcggcaa cctgctggag aatatagtgc gggatgcaat taatgctttg attcctggtt
     1801 gggtgccgga ctgggcgaag gatctgcttt gggcaatcat cggacccatc ctcgacctcg
     1861 tcaaaggcct tctcggcatt gcaggtgaaa tcaacgactg gttatcggat ctgctgggca
     1921 acaggttcga tcttttaggc gccatcgaaa ccgccgtcgc ggattacttt gccagccagt
     1981 acccgattta tgaatttgaa gacccctatc caatattgcc agccgcttca gggcttattc
     2041 cggtgaaaat cccactgcgc gatctggacg ccaaggttga ctcgaaagag atgacagtca
     2101 ttgccaatat cggagcatag ccatgaaaat gaatttcgat cctaactttt tctcacaggt
     2161 cgggctggat agcggtgcac tgacaatgct cggagcagtg gtccatagtt tttgcgagcc
     2221 gggcgaatac cgcggggtcg cgcgccgtgg agcggagccg gaagcggtat tttacatttc
     2281 tgtcgacaag aattcacccg ttgcccaggc gaatatcgat ctcgcagcac tggttcagga
     2341 agagcctgcc ggcgaatctg aaaatggctg ttgcccgggc ggccagggaa attattttgt
     2401 cgtaaatccc aagggctatg cagtctttca cgtttccagc ggtgccggcg gttattcagt
     2461 acacgtcaga aagaatgacg aagatccaaa gacaaagata ttcgatagcc gggagctgga
     2521 tgcgggagac attttctcgg ccattatcat ccgccctggt acctactcgg tcacaaatct
     2581 cgtgactgga agcaagagcg aggtgattgt ttcttatccg aagcctggca aaactcccta
     2641 ccggccgcca gctccaatag cggtggactg cagcgagcgc gagatgacgc tgggacgtat
     2701 cgagttacaa ccggggcaag gccttaactt ccacttgaaa accaggtcgc ggatcaagat
     2761 cgaactggtg aagccggacg atggtcccgg caagtcgcgc gaacccgagc gaaccgggtg
     2821 gatcaagcgt accctcgcac acgagtaacg cacattgggg tcgcaccggg gtagttggcc
     2881 ttgagcgcga tttcaatcgg ggagaggcct ctgccgccta cactcgttta tggctatata
     2941 gagggacagc tttcgaggtt ggtttcccga ttcggcctcg aggaccaacg ggctgcaatt
     3001 ctgggaacgt atcagctcat ctgtgttgag tcgcttgcca ttccgcttgc ccagaggcca
     3061 ccaagttttt cccgcatcaa cgaggatgga acgccctttc agttctcact agcgctaggt
     3121 gcttcgaaat cgccactaca atttctaagc gaggcaggag tggctgggga tagtgctgct
     3181 gcccgccgtc gtgcgaacag cgaagcgctg cattggattg ccactttgct tgacgcccag
     3241 gaagcgctgc ttgaagtgac tcccctgatt gatgagatgg taccggggaa taatccggat
     3301 ttgctggcgg actcgtctgg ggcaatctgg ataggagcgg cttttgtaca agaagaagcg
     3361 ccgaagctga aaatttacct gaatgccaaa tgggggcgag aggcggatca gtggaaacgc
     3421 ctgcattcct tcgcttctca ttttaacgct gcttcccggt ggggtgacgc taagccggcc
     3481 ctaaagcggg agatgcagcc tctgggtctc tcaataacga taagcggagg ccaactcccg
     3541 agcggacgca tctacctcag cgcgtatggc aagccgatgg cttactatga agcgctcgca
     3601 gaagcgagtg ctgggagagc ttttctggag cgattcagac tttatactga tatattgctc
     3661 ggcgaaaaca ggcaatatcc aaccccgtcc accgtatgtt ctttcggttt tgatgcctgc
     3721 ggctcgtatg atttcaagtt tgaaatgtgc ggcctccgaa tcttcgaaag cgacgccgat
     3781 gccatggcac gctgtgtcaa gtggctgaag ctgcagaata tcgagccgaa cccgtacctg
     3841 catgtcctgc aggaactgtc aggagggatg ttttgcagtt caaagccccg cttgcactgc
     3901 tatttgggac ttggggccaa gcgggagagc atttattcca cgttttattt taatccgggc
     3961 atctggaacg cttgagcgaa aatttcaaga ctgagttgcg agaagcaatc agagcaggta
     4021 ttcgctacgt tctgcaacgc caacaggcca gtggttgctg gatagattgg gaattgcccc
     4081 caggcgaatg tcgaatgtgg accactgcct acgtaggctg caaattaagc agccttcctc
     4141 cggatttgag tcaggaaacc ttcgtggcaa gaaaaagggc cgctcgctgg ctgctcctca
     4201 accaattgcc gggctatggc tgggggtatg gcgatacagt cggagcagat gctgattcaa
     4261 ctgcccacgc cattcttttc ttatcggcgg aaggtactcg cttatctgag gacagctacc
     4321 gccgtctgga agtgtttcaa tccgaagatg gaggattttc tacctatctg aggggagata
     4381 gccatggatc gtggtgcgtt tctcatccag atgtcacccc aatggctctc ctcgcgctct
     4441 taaccaggca tgttgaagga agcgtagcga tcaatcgggg g
//
LOCUS       DASSHJ010000111         8740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_881069, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000111 DASSHJ010000000
VERSION     DASSHJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881069"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VFI43_04425"
     CDS             complement(<1..1079)
                     /locus_tag="VFI43_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7061404.1"
                     /translation="MTPALVGELKKFLPADRILTDPVDCFAYAYDNSRRICPPDGVIF
                     PVNIDEIRRVVELCNNHGVPLTPRGRGTGTSGGSLPEQGGIALSLERMTRVVKIDPQN
                     RVIVAEPGVINQSLQDVVKPFGYFWPPDPSSAAYSTIGGNLATSAGGPHAVKYGTTRD
                     HVLGLKAVTGGGDVIRTGCYTTKGVVGYDLTRLLVGSEGTLAVIAEATLKLMPLPEFV
                     GGVTAYYQDLKSCTEAIVAVMALGQTPSALEFLDSGSLDLIRSRFSDIVPPGSQAMLM
                     IEADGTERDVAETCTAILSACQNEGLIVGGITENPAVLWRARKTLSPLLRDIAPKKIN
                     EDVVVPVSMLPRFLEEVSSLSREFQV"
     gene            complement(1191..1775)
                     /locus_tag="VFI43_04430"
     CDS             complement(1191..1775)
                     /locus_tag="VFI43_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7061405.1"
                     /translation="MKRFNHSGLLLAVLFAPLVSGCVLLAAAGVAGVGTGVAMSQDRR
                     TSGVFVEDEAIEMKGGQRISERYGDNVHVNVTSFNRNVLLTGEAPTEAAKADIANLVK
                     GVEQVRSVVNEITVGPPSTFGSRSNDTLITSKVKGRFVDGGKFQVNHVKVVTENGIVY
                     LLGLVNRNEAASAVEIASSTSGVRKVVKVFEYTS"
     gene            complement(1902..2240)
                     /locus_tag="VFI43_04435"
     CDS             complement(1902..2240)
                     /locus_tag="VFI43_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET7061406.1"
                     /translation="MNGNEAERSAERFLIQQNLVPVARNYRCRLGEIDLIMRDGGEIV
                     FVEVRMRRNALFGGAAGSITRVKQKKLLLAAKYYLAGLNNIPPCRFDVVLLTGNEGER
                     MEWIKNAFEE"
     gene            2426..3283
                     /gene="rsmI"
                     /locus_tag="VFI43_04440"
     CDS             2426..3283
                     /gene="rsmI"
                     /locus_tag="VFI43_04440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7061407.1"
                     /translation="MYIVGTPIGNLGDITLRALETLGAVGMIAAEDTRIASRLLDHYS
                     LSKTLIPLHQHNEAATVKKIAGALSRGISVALITDAGTPGISDPGALLVNRIRHLGYH
                     IVPIPGPNAAVCAFSAAGIIAPHFFFYGFLPASGQARRRELRNLVSLPYTLVFYEAPH
                     RILDSIGDIQEILGERRQITIARELTKLFETIHVCPLAEAIEWLQADPNRQKGEFVLL
                     LSGAETVKERGEQNLSDQAQHTLKALLDALPLAQAVRLAADITGESRRSLYAHALLLR
                     NGQVEKKTT"
     gene            3450..3689
                     /locus_tag="VFI43_04445"
     CDS             3450..3689
                     /locus_tag="VFI43_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7061408.1"
                     /translation="MKTRAVYYHAGCPVCVEAENQVADALDTSRYEIERVNLGEQKDK
                     IAEAEASGVKSVPALVINGAPFHINFGAPMSALKS"
     gene            3964..4434
                     /locus_tag="VFI43_04450"
     CDS             3964..4434
                     /locus_tag="VFI43_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7061409.1"
                     /translation="MELILWRHAEAEEGGLDSMRKLTHKGLKQAQTMAKWLKQRLPDE
                     TVVMASPTMRTQQTAAALKDDFQTIEEIGPGATAKAVLAAAGWSQGEGTVLIVGHQPT
                     LGQAAALIISGTSAPWIIRKGSIWWFLQKEKRGDRGEVVGREIVLKAAISPEMI"
     gene            complement(4537..4983)
                     /locus_tag="VFI43_04455"
     CDS             complement(4537..4983)
                     /locus_tag="VFI43_04455"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7061410.1"
                     /translation="MRADTLIGENVYNPEGIELGEIKDIMLDVKAGTIAYAVLSFGGF
                     LGIGDKLFAIPWKALHPDEVYKRFILDVDKERLKSAPGFDKDKWPDMADPTWQNKVQS
                     YYGTKPFKEPTDSPDIPYANTPYTVPSAADTAKPGSSGNMDSGRVR"
     gene            5390..7273
                     /locus_tag="VFI43_04460"
     CDS             5390..7273
                     /locus_tag="VFI43_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HET7061411.1"
                     /translation="MQQSPGSPHQSLALVSLGALGVVYGDIGTSPLYVIKTVFDPVHG
                     LPVNELNVVGIISLVFWALMAVVTLKYITLILRADNHGEGGIMALLSLASSSVATHPR
                     LRGALFLIGGFGAALFYGDGVITPAISVLSAVEGLEVATPLLQPYVLPITLVVLIALF
                     LFQQRGTGGIGAVFGPIMLLWFSTLALAGVVNIIAAPQIVAALNPVHAVAFGMNNGWL
                     AFIALGAVVLAVTGGEALYADMGHFGAVPIRVAWYTCVLPALTLNYLGQGALLLSNPA
                     AISNPFYLLFPSWALYPAVGLATLATVIASQSVISGVFSMTKQAIQLGFLPRMQIRYT
                     SDRKIGQIYMPFVNWALLAVVIMAVLGFGSSSNLASAYGFAVTATMVIETLLTFFVLR
                     FAWKYPLLLSLLPTAFFLTIDAAFFTSTTLKLAQGGWFPLAIGTIIFSIMITWNRGRQ
                     ILLEHLRSAAIPLHPFLQSLFASPPVRVSGTAVFLTADPAGVPHALLHNLSHNQVLHE
                     RVIFLTVEYLEIPLVPVHNRLSVTPLIENCYQITVRYGFKDQPNLPYALELCQPYGLV
                     FEPLSTSYFLSREIVVPSPGTGMAQWRERLFAAMARNASNAAEYFKLPANRVLELGAR
                     VEI"
     gene            7322..>8740
                     /locus_tag="VFI43_04465"
     CDS             7322..>8740
                     /locus_tag="VFI43_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061412.1"
                     /translation="MSEARKRPYFKHSIVQLEKLFDAGQSDTHILNLLAHELEFRTTD
                     RAVKLRVTVAEVLATASIKRANVARASGATSPAGHATSVIAFPKEPDPQPVQMGEQAT
                     PRKSVAPPAPVYLGELPSVPIPKGINEPSGILAAWIALEALSPQTYRRPDDLAAGDRS
                     CVVDLSSGRMPWGTGERSRRKRQLYYQVVLGAIPMDRATEAVVKAFGEDEEPASRARE
                     KAAIAAVLVDKNGVPVEENGIAVSSFGWALPLVLKLKLGALGVWPKIEPKIIQKLDDI
                     LRRVDEDGTALPLDFETIEKAHRWLVTQFGLPDELIEAPTFALRVYHYFKAKNPPEAA
                     LLNSFFLSDLARGATLVGQGSAPASLRRYLGIDKPAQTFDLFADRTTLEKAVAPAMMP
                     AARWPSSGGHSLVLLQQAAVNLARSELADGDGIVAVNGPPGTGKTTLLRDVVAACVLD
                     RALAMATFEDPEKAFTPTGERMS"
BASE COUNT         1934 a         2496 c         2282 g         2028 t
ORIGIN      
        1 acctgaaatt cacggctcaa actgctcact tcttcaagga agcgcggcag catcgataca
       61 ggcacgacta catcctcatt gattttcttc ggggcgatat cccttaatag cggcgatagg
      121 gttttacgag cgcgccaaag cacggcggga ttttccgtta tgcctccgac tatcaaccct
      181 tcattctggc aggccgaaag aatggcggta caggtttccg caacatcccg ttccgttccg
      241 tctgcttcta tcatcagcat cgcttgggaa ccaggaggta cgatatctga aaatcgactg
      301 cggataagat cgagcgaacc agaatcaagg aactcgagtg cgctgggagt ttgaccaagg
      361 gccatgaccg caacgattgc ctccgtacag cttttgagat cctggtagta ggcggtaacg
      421 ccgccgacaa attcaggaag gggcatcagc ttgagtgtcg cttcggcgat aacggcgagt
      481 gtgccttccg agccgaccag caagcgggta agatcgtatc ccacgacgcc tttcgttgtg
      541 tagcagcctg tcctgatgac gtctccgccg cctgtcaccg ctttaagtcc caatacatga
      601 tcgcgggtgg tgccgtactt cactgcgtgg ggcccgcctg ccgacgtcgc caggttgcca
      661 cctatggtcg agtaggccgc actcgacgga tcaggcggcc agaagtaacc aaagggcttg
      721 accacgtcct gaagcgactg attgatcaca ccaggctcgg caacgataac gcggttctga
      781 gggtctattt taacgacccg cgtcattcgt tcgagtgata gtgcgatacc tccctgctcc
      841 ggaaggctcc ctcctgaagt gcccgttccg cgaccgcgcg gggtgagggg aacgccatga
      901 ttattgcaaa gctccaccac ccgccggatt tcatcaatgt tgacgggaaa tataacaccg
      961 tcaggtggac agatccttcg gctgttgtcg tatgcatagg caaaacaatc caccggatcg
     1021 gtcagtatcc gatcagccgg caggaatttt tttaattccc caacaagggc gggcgtcaca
     1081 ggagatttca gtcccgtcag cacatccaga agtgggattc gccgacggaa ccagcaaaga
     1141 cgggagctat ctcaaaaaat accctctccg tccacactct ccgcatcatg tcagctcgta
     1201 tactcaaata ccttgaccac cttccgcaca ccgctggttg agctcgctat ctccacggca
     1261 ctggccgcct cattccggtt caccaagccc aggagataaa caatcccatt ttccgtcaca
     1321 actttcacat ggtttacctg gaacttgcca ccatccacaa accgcccttt gaccttggag
     1381 gtaatcaggg tgtcgttgct ccttgagcca aacgtacttg gaggccccac ggttatttca
     1441 ttcacgacag agcgtacctg ctcaacaccc tttacgagat tggcgatatc cgccttggcc
     1501 gcttctgtgg gcgcctcccc cgtgagcagg acgttccggt taaaactggt aacgttgacg
     1561 tgcacgttat cgccatatct ttcgctgatg cgttgaccgc ctttcatttc aatggcttca
     1621 tcctcaacaa agactccgct cgtgcgccga tcctgggaca ttgcaacccc ggtaccaacg
     1681 ccggcaacac ccgctgccgc aagcaggaca catcccgata caagcggagc aaacaaaacc
     1741 gctaacagca agccactatg attaaacctt ttcattcaat ccctctaaag tcaatttcat
     1801 attccaaaat acccctacac tgcaactaac ctccagaata cgcgcggatt tccttactgt
     1861 gcttccgaat ccactttcaa gacaccaggc gtttttgcgc atcattcctc aaacgcattt
     1921 tttatccatt ccattctctc accctcgttt ccagtgagta aaaccacatc aaaccggcag
     1981 ggtggaatgt tgttcaagcc cgcgagataa tacttggctg caagcaataa tttcttctgt
     2041 tttacgcgag tgatgctgcc tgccgcgcct ccaaataaag catttctgcg catgcggacc
     2101 tcgacaaata caatctcccc tccatccctc atgatgagat cgatctctcc caggcggcaa
     2161 cggtaattcc ttgcgaccgg cactagattc tgctgtataa gaaacctctc cgcgcttcgc
     2221 tcggcttcgt ttccgttcaa tcggtttgtc cccggacccg ttgcaggtgc cccgaccggg
     2281 gcgcagaaac gagtccacgt aattaaagga cgacatttta caattctacc cttatccttt
     2341 catcactctt catttagcat gcaactgtca gttgcaaagt gccctctcca acatgcagac
     2401 cgcatctata atttcgccag gaatcttgta catcgttggt acgcccatag gaaatctcgg
     2461 cgacatcacc ctgagggcgc tggaaacgct gggggccgta ggcatgatag cagccgaaga
     2521 cactcgtatt gcatcacgtc tgctcgacca ttattccctt tcaaaaacgc ttattccctt
     2581 acatcaacat aacgaggcag caaccgtcaa aaaaatcgcg ggagctcttt cgcggggcat
     2641 ttccgttgca ctcattactg acgcgggtac gccgggaatc tccgatcccg gggccctcct
     2701 cgtcaatcgc atccgccatc tcggctatca tatagtgccc ataccgggcc caaatgcggc
     2761 ggtatgcgcc ttttccgccg ccggcattat cgctcctcat ttcttttttt atggttttct
     2821 tccggcttcc ggacaggcaa ggcggcggga attgcggaac ctggtttctc taccgtacac
     2881 ccttgtcttt tatgaggccc ctcatcgcat cctcgactct atcggcgata ttcaggagat
     2941 actgggggaa cggcgtcaga tcaccattgc cagagagttg accaagctgt tcgagactat
     3001 ccatgtctgt cctctcgcag aggctatcga gtggctacag gccgacccca ataggcaaaa
     3061 aggcgaattc gttctcttgc tgagcggggc ggagaccgtc aaggagagag gagaacagaa
     3121 tttgagcgac caggcccagc ataccttgaa agcactgctc gacgcattac cgcttgcgca
     3181 ggcagtaaga ctcgctgcgg acatcacggg cgaaagcaga agaagccttt atgcccacgc
     3241 cttgttactt aggaacgggc aggttgaaaa gaaaaccaca tgaagattat catttcgtca
     3301 agttgacccg gatgcagcca gagaggtggc gcggagcaag gtgagccatt ataatagcgg
     3361 cttcacccga gagcagaacc ttacgttcat gcacccgagg cgaacgtggg aagtccccca
     3421 ccgataatta atcactggag agatcgacta tgaagacacg tgcagtttat taccacgccg
     3481 gttgtcccgt gtgtgtggag gccgagaatc aggtggccga cgcgctggac acctcccgct
     3541 atgaaatcga gcgggtaaat cttggcgagc aaaaggacaa gatagccgag gcggaagcat
     3601 cgggcgtgaa gtcagtcccg gcgctcgtaa ttaatggcgc gccttttcat atcaacttcg
     3661 gcgcaccgat gtctgcattg aagtcataac cagcaaaatg gcgggcaatg cccgcctttt
     3721 ggccctgcct ttcgtggctt tttccccccg tttcagccgc ttcaatccgc tttccgccgg
     3781 gaaatcccac gtcaaaaccc tcttggcacc ttccgtttac ttcaatttct ttccgtactg
     3841 cggcatattt ttcaccgctt agtcggatcg cccggttacc tgagttccgg cattttctgt
     3901 cgagccctgc atcgcaattt agctatactt aaccctgaca ctcaaggcga tggggagata
     3961 taagtggaac tgatactatg gcgtcacgcc gaagccgaag agggtggcct tgacagcatg
     4021 cggaagctga cgcataaagg cttgaagcaa gcgcagacca tggccaagtg gcttaagcag
     4081 aggctgccgg atgaaactgt cgtaatggct agcccgacaa tgcggacgca acaaacagca
     4141 gctgcgctta aggatgattt tcaaaccatc gaagaaatcg gacccggtgc aacggcaaaa
     4201 gcggtgcttg cggcggccgg ctggtcgcaa ggagaaggta cggtacttat cgttggtcac
     4261 cagccaacac tcggtcaagc ggcagcgctc atcatatcag gcacgtctgc gccctggatc
     4321 atacgcaagg gttcgatatg gtggttttta cagaaagaaa aacggggtga tcgtggggag
     4381 gtggttggca gggaaattgt cctgaaggcg gcaatttccc ctgaaatgat ttaacgcccg
     4441 cggaggccca acccttcgac agccggttga acgggagcag tttggcgcga gaagctcacc
     4501 cccgcggctc cgcctctcgc cgcttttctc agatattcaa cgaacccttc ccgaatccat
     4561 attgccgcta ctgcctggct tggccgtatc agcggccgag ggtaccgtgt aaggggtatt
     4621 ggcgtaggga atatcgggtg aatcggtcgg ctccttgaaa ggcttggtcc cgtagtatga
     4681 ttgaaccttg ttttgccatg taggatccgc catgtccggc catttatcct tatcaaatcc
     4741 aggggccgat ttgaggcgct ccttgtccac atcaagtatg aaccgcttgt atacctcatc
     4801 cgggtgcaac gccttccatg gtatcgcaaa aagtttgtca ccgataccga ggaagccccc
     4861 gaaggatagc actgcgtaag caatggtacc ggccttcaca tccagcatga tatccttgat
     4921 ttctccgagc tcgatcccct cggggttata gacattctct ccgatgagcg tatcagcccg
     4981 catcaacctc ggttcctgag agttctcacc tgacgattga ggtgtgtttt gggcagtgct
     5041 gctttgaaat tccataatta tctcctcgtt ttacaataat cagtttccaa tgatctcgac
     5101 atcgtcacac ggtaaagaat aagcaagtac gtggacgtga tcagtacggt aaccaacaaa
     5161 gacagcgttg tagatcagcc tcggggaagc gtgatgaggt acactaggac aggtaacacc
     5221 tgaaagtccc acccgggtcc agggatccat actcttgata tcgcagtccg tggcgaaggg
     5281 gttggtgtat cgactccgtc atcctgctgt tacagccact tgcgagaatc tgttctgaag
     5341 tggcttaagg gcgtccccgc ctgaaaactc catctaacga gcgaaaacaa tgcaacaaag
     5401 ccccggctct ccccatcaat ctctggccct cgtcagtttg ggggcccttg gtgtggtcta
     5461 cggggacatc ggcacaagtc cgctctatgt cataaaaaca gtttttgatc ctgtgcatgg
     5521 cctgccggta aacgagctaa acgtcgttgg aatcatttcg ctcgttttct gggcgctcat
     5581 ggcggttgtg accctcaaat acatcaccct gatacttcgg gccgataatc atggagaagg
     5641 cggaatcatg gcgctgcttt ccctcgccag ttcctcggtg gctacacatc ctcgattgcg
     5701 aggggcgctc ttccttatcg gaggttttgg cgctgctttg ttttatgggg atggcgtgat
     5761 cactccggcg atttcggtgc taagcgcagt ggaaggactg gaggtcgcca ctccattgct
     5821 ccagccgtac gtcttgccga tcacgctggt ggtgttgatt gcgctcttcc tgttccagca
     5881 gaggggaacc gggggtatcg gggcggtttt cggcccaatc atgcttctat ggttctcgac
     5941 gctcgcgctt gccggggtgg tcaatatcat cgccgctccc caaattgtgg cagcgctgaa
     6001 tcccgtccac gctgtcgctt ttggcatgaa caacggctgg cttgcattta tcgcgcttgg
     6061 agcggtggta ctggcggtga ccgggggaga agcactttat gccgacatgg gtcattttgg
     6121 ggcggttccg atccgcgtgg cttggtatac ctgcgtcctg cccgcgctca ccctgaatta
     6181 tctcggacag ggcgccctgc tgctttccaa ccctgccgcg atttccaacc cattctatct
     6241 gctgtttccc tcttgggcgc tttatccggc ggtcggtctg gcaactttgg cgacagtgat
     6301 tgcatcgcaa tccgttatct ccggtgtttt ttcgatgacc aagcaagcaa tccagctcgg
     6361 atttttaccg cgcatgcaga tcagatatac ctccgatcgg aagatcgggc aaatctatat
     6421 gccgttcgta aactgggcct tgcttgctgt agtgatcatg gcagtgctgg gatttggttc
     6481 ttcgtccaat cttgcctctg cttatggatt cgccgtaacc gcgacgatgg tgattgaaac
     6541 gctgctcact ttttttgtat tgcgcttcgc gtggaaatat ccattacttc tgagtctgct
     6601 cccgacagcg tttttcctga cgatcgatgc tgctttcttt acctccacta cactaaagct
     6661 tgcgcaagga ggctggtttc cgttagcaat cggcacgatc atcttttcca tcatgattac
     6721 atggaatcga gggcgacaga ttctactgga gcacctgcgc tccgcggcaa ttccgctcca
     6781 cccttttctc cagtctctct tcgccagtcc cccggtacgg gtatctggca cggcagtatt
     6841 tctgacggct gatcccgctg gagtgccgca cgcgctgctg cataacctct ctcacaacca
     6901 agtcttgcac gaaagggtca tatttctgac agtggaatac ctcgagattc cgctcgtgcc
     6961 ggtacataac cggctatcgg taaccccatt gatcgagaat tgttatcaga tcacggtcag
     7021 atacggattc aaggaccagc ccaatcttcc atacgcactc gaactatgcc agccctacgg
     7081 actggtattc gagccactga gcacatcgta tttcctgagc cgtgagattg tagttccaag
     7141 cccgggtacg ggcatggccc agtggcggga aagactgttt gcggcgatgg cacgtaatgc
     7201 cagcaacgcg gccgaatact tcaagctacc cgctaaccga gtgcttgaac tgggtgcgcg
     7261 agtcgaaatc tgaggggtcg gcgtcgacaa aacacaccaa gaaaacgagg atgactaagg
     7321 cgtgtcggag gctagaaagc gcccatattt taagcactcg atcgtccaac tcgaaaaact
     7381 cttcgacgct gggcaatccg atacgcatat tctcaactta ctggctcatg agcttgagtt
     7441 ccgaacaacg gaccgggcag taaagctccg tgtgaccgtc gcggaagtcc ttgcgactgc
     7501 atcaatcaag cgtgcaaatg tagcccgcgc tagcggcgcg acctcaccag caggtcatgc
     7561 gacctccgtc attgcatttc ccaaggagcc tgatccccag ccagttcaaa tgggggagca
     7621 agctacgcca cggaagagtg ttgcgccccc ggcgcccgtg tacctcggtg aactaccctc
     7681 cgttcccatc cccaagggca taaacgaacc gtcgggcatc ctggcggcat ggatagcgtt
     7741 ggaagctctg tcaccgcaga cttatcggcg cccagatgat ttggccgccg gagaccggag
     7801 ttgtgtcgtc gacttgtcga gcggccgcat gccttggggg acgggagagc gttcgcggcg
     7861 aaagcggcag ctctactatc aggtcgtact cggtgcgatc ccaatggacc gagcgaccga
     7921 agcggtggtg aaagctttcg gtgaagatga agaaccggcc tcaagagcac gtgagaaggc
     7981 cgctatcgct gcagttctcg tagacaagaa cggcgtgcct gtcgaggaaa acggtatcgc
     8041 tgtttcgagt ttcggctggg cattgccgtt ggtcctcaag ctgaagctcg gtgcgttagg
     8101 agtttggccg aagatcgagc cgaaaatcat ccagaagctg gacgatattc tgcgtcgcgt
     8161 ggacgaagac ggcacggctc ttccactcga cttcgagacg atcgagaaag cccatcggtg
     8221 gctggtgacg caattcgggt tgccggatga attgatcgag gcgccgacct tcgcactgcg
     8281 tgtttatcac tatttcaaag ccaagaatcc gcccgaggcg gcgctcctca actccttctt
     8341 tctaagcgat ctcgctcgcg gagccacact tgtcggccaa ggaagtgcac cggccagcct
     8401 tcgcagatac ctcgggatcg acaagccggc gcagacgttc gacctgtttg ccgatcgcac
     8461 aacgctggaa aaggctgtcg ccccggcgat gatgccggcc gcccgatggc caagctccgg
     8521 cggacactcg ctggtgctgc tgcaacaagc cgccgtcaat ctcgcgcggt cagagcttgc
     8581 tgatggtgat ggcattgtcg cggtgaatgg accgccgggg accggaaaga ccacgttact
     8641 tcgcgatgtc gtggctgcct gcgttctcga tcgcgccctt gcgatggcca ccttcgaaga
     8701 tccggagaag gcttttacgc cgacaggcga gagaatgtcg
//
LOCUS       DASSHJ010000112         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_724603, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000112 DASSHJ010000000
VERSION     DASSHJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724603"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFI43_04470"
     CDS             complement(<1..782)
                     /locus_tag="VFI43_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HET7061413.1"
                     /translation="MLSEVLPRTLPAQPFFLETTTGKRFCLYHAPGADTQCLGVFIYI
                     HPFGDEMNKSRRMAAMQARAFAKTGFGVLQIDLYGCGDSEGDFGDAKWDIWKNDLMFA
                     CNWIASRTQAPVNLWGLRIGALLALDFASEHEKVLGKIVLWQPVVDGASFLNQFLRLR
                     IASEMFLNAGAPEMATGTKALRERLMNGESLEVAGYELSPHLAGAIEKCKAADLAVQT
                     CAMHWFEIVAEPGRSISPAGTKVVNQWKLNGVNAQVHAVPGLP"
     gene            complement(772..1023)
                     /locus_tag="VFI43_04475"
     CDS             complement(772..1023)
                     /locus_tag="VFI43_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7061414.1"
                     /translation="MQHTEEVKNILSDVLSLGERKNALREDSVLLGELPELDSMAVVN
                     VITALEDHFGISVDDDEISAKTFETLGSLSEFVEQKLAE"
     gene            complement(1037..1738)
                     /locus_tag="VFI43_04480"
     CDS             complement(1037..1738)
                     /locus_tag="VFI43_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061415.1"
                     /translation="MLQQLGWFNTSLFVLNKILAAVSKGRLCVYRYHLIAQPVAKEAL
                     VPRGRGNKIQVRLIQENDEIIKEFPRPSAAIQSRFEQGAKCLVALKDGEFAGFLWLLM
                     GSYQEDEVRARYVPLPLEQSAWDFDVYVAPQFRLGLTFLRLWDHANRMLSENGISWSC
                     SRISAFNSGSLGAHSRLGTLSLGSAIFVCAGNWQITFATLSPFVHLSRHAGSFPEFRL
                     STRGLVTNQSPHVAN"
     gene            2321..>3483
                     /locus_tag="VFI43_04485"
     CDS             2321..>3483
                     /locus_tag="VFI43_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7061416.1"
                     /translation="MTYSIPVFITQAHANTFERLSSLLADTPSGGWVQANVNVFSDAW
                     PTGADAVQVQSHQFPGAIVSAWSSFAWDPTNARLMLFGGGHANYSGNEVYIWDGATGN
                     WGRGSLPSRVDANGFVVDAAAPQSAHTYDNNIYLPVNGMFLTFGGAAAPGGGNFQELN
                     GTTISRAGPYVWDPLKADPMKVGGTTGSGWNTTNVAQGGQMWMDREGQWSGVEPGQYV
                     NGTAAYRTENGHDVVYLTAEGGSASGFPSLYRYTLGDIRNGGTDTWERIGVMNNSVGF
                     QGTGTIDTEHNLYIKTATVIGSYTSDLTIWDLSKANGANPNLNQDIGINLVNSDGSDF
                     VMNGNYGIDYDPVNNTIVLWDGRNGGGEVWWANVATDPNGKIETTWVIHKVESS"
BASE COUNT          869 a          917 c          912 g          785 t
ORIGIN      
        1 ggcagaccgg gaacggcatg cacttgtgca ttcactccat tcagcttcca ctggttgaca
       61 accttcgttc ccgcgggtga gatggaacgg ccgggttcag cgactatttc gaaccaatgc
      121 atggcacatg tttgaactgc cagatcagcc gctttgcact tctcaatggc gccagccagg
      181 tgcggcgaca gttcataacc ggcaacttcc aggctttccc cattcataag gcgctcgcgg
      241 agcgcctttg ttcccgttgc catttccggg gcgcccgcgt ttaaaaacat ttcgcttgcg
      301 atacgcagtc ttaaaaactg gttcaggaag gacgctccgt ccacgaccgg ttgccataga
      361 actatcttgc cgaggacctt ttcatgctcg ctggcaaagt ccagggcaag caacgcgccg
      421 atgcgcaaac cccatagatt tactggcgcc tgcgtccggc tggctatcca gttgcaggcg
      481 aacatcaggt cgttcttcca aatatcccat ttcgcatcgc cgaagtctcc ttcactgtct
      541 ccacagccgt aaaggtcgat ctgcaacacg ccaaaacctg tcttggcgaa tgcgcgcgcc
      601 tgcatcgccg ccatccggcg tgacttgttc atttcatcgc caaacggatg tatatagatg
      661 aagacaccga gacactgtgt atcggcacca ggggcatggt agagacaaaa acgctttccg
      721 gtagtcgttt ccagaaaaaa aggctgggcg ggaagagtgc gaggcaagac ttcactcagc
      781 aagcttctgc tccacgaatt ccgacaaact tccaagcgtc tcgaaagttt tcgcactgat
      841 ttcgtcatca tccacactga tgccaaaatg gtcttccaac gcagtgatca cattgacgac
      901 cgccatggaa tccagctccg gcagctctcc aagcaagaca gaatcttccc tgagagcgtt
      961 ttttcgctca cccaggctca ggacatcgga caaaatgttt ttaacttctt cagtgtgctg
     1021 catcacggct ccatggttaa tttgccacgt ggggcgattg gtttgttacg agtccccggg
     1081 tgctgagccg gaattccgga aaggaaccgg catgcctgga taaatgaacg aaaggcgata
     1141 gcgttgcaaa tgtgatttgc cagttccccg cacaaacgaa gattgcgctg cccagggata
     1201 aggttccaag gcgtgaatgg gcgccgagcg agccactgtt aaacgccgat atcctgctac
     1261 aagaccacga aataccattt tcactcaaca tccggtttgc atgatcccac agccgaagaa
     1321 aggtcaaccc caggcggaac tgtggcgcga cgtacacatc gaaatcccag gctgattgtt
     1381 ccaacggcaa gggcacgtat cgggcacgca cttcatcttc ctggtaactc cccatcagca
     1441 accatagaaa acccgcaaat tccccatcct tcaaagcgac caggcacttt gccccttgct
     1501 cgaatcggga ttgaattgcg gcggaaggcc tggggaactc ctttattatt tcatcgttct
     1561 cctgtatcag gcgcacctga attttattgc ctcttccccg cgggacaagc gcttcttttg
     1621 cgaccggttg agcgatcaga tggtatcggt atacgcagag gcgccctttc gagacagcgg
     1681 ccagaatctt gttgagcacg aaaaggctcg tgttgaacca gcccaattgc tgcaacattt
     1741 tatatagctc ttcctttcga gctgctatcg atggcattcc gggactttga cggttaaggc
     1801 ggcggagaag acgaccagga tggaaattta aatacggctc cggaagagct gagcatttgc
     1861 aattgataag gtcttggtaa aatccattcc ctgctgctgt ccgttcagcc tcttcccgca
     1921 cagcaacagg accccataga ggacgaggag caggctagcg taattttctt ggagcggctt
     1981 atgcccgaaa ggccttacct gtacttaagg acaatagtta ttcgaatgaa aatcgcaaga
     2041 caatactata agtcatgtgt tgagcgaaag tcgaacagct ttgcttttca tgccttttct
     2101 ttgccctgcc tgccgggtgc aaaggtcgaa aataaacgaa atttgtaacg ttagggttgt
     2161 ttcgagagtt cagatttatt caattcaagt tcagaggtag gaaatgtata acaagagcat
     2221 acaaaatcaa gagggcaaaa aaaaatcagc tttttcccaa agttttaaaa gccccggcgg
     2281 attaagccgg attgcgacac tggtcgcagc ttctttactg gtgacttatt cgatccctgt
     2341 attcattacg caggcgcatg cgaacacctt cgagcggctg tcatcattgc tcgccgacac
     2401 tccgtcaggc ggatgggtgc aggcaaacgt aaacgtcttc tccgacgcat ggccaactgg
     2461 agccgatgcc gtgcaagtgc aaagccatca atttccggga gctatcgtaa gcgcctggag
     2521 ttcattcgcc tgggacccaa ccaacgcacg tctgatgctc tttggaggcg gacacgcaaa
     2581 ctactcgggt aacgaagttt acatctggga tggtgccacg ggaaattggg ggcgcggctc
     2641 gctacccagc cgcgtggatg caaatggttt tgtcgttgat gccgctgcgc cgcaatccgc
     2701 gcatacgtac gataacaata tttaccttcc cgtcaatgga atgttcctga cgttcggggg
     2761 cgctgcagcc ccggggggag gaaattttca ggagctcaat ggaacaacga ttagccgggc
     2821 aggcccctac gtatgggacc ccctcaaggc agacccgatg aaagttgggg gcaccacggg
     2881 atcaggctgg aatacgacaa acgtggcgca aggcggccag atgtggatgg atcgggaagg
     2941 gcagtggagc ggggtggagc ctggacaata tgtcaatggt acagcggctt atcgaactga
     3001 aaacgggcat gacgttgtct acctgacagc cgagggaggc tctgcctccg gatttccctc
     3061 gctctatcgc tatacgctcg gcgacatcag aaatggcggc acggatacct gggaaaggat
     3121 tggcgtcatg aacaattcgg tcgggttcca ggggacagga acaattgata ccgagcacaa
     3181 cctttacatc aaaactgcga cggtcatagg atcttatacg tccgacctca ctatttggga
     3241 cttgagcaaa gcaaatggag ccaacccgaa tctcaatcag gacattggaa tcaacctggt
     3301 caatagcgat ggcagtgatt ttgtaatgaa cggaaattac ggcatcgatt atgacccggt
     3361 caacaacacg atcgttttat gggacggtcg caatgggggg ggcgaggtat ggtgggcaaa
     3421 cgttgccacc gacccaaatg gaaagatcga gacgacctgg gttattcata aggtcgaaag
     3481 ctc
//
LOCUS       DASSHJ010000113         6338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_672689, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000113 DASSHJ010000000
VERSION     DASSHJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672689"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFI43_04490"
     CDS             <1..351
                     /locus_tag="VFI43_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7061417.1"
                     /translation="VPVPCTPAFVRGIVSVRGEILPVIDLKKFFELPEKELSDLSTLI
                     VLHMGEIMFSILADKIVSVRRIVAADIQPPLPTLTGIRKNYVKGITSERLVVLDAAKL
                     LMDESLVVEEQVGE"
     gene            417..1913
                     /locus_tag="VFI43_04495"
     CDS             417..1913
                     /locus_tag="VFI43_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7061418.1"
                     /translation="MSISKKIIGGYGIVLALLALSTAVAFYALNQRQAAYEHFLRVDE
                     RLMESANELRIAAFAQQTYIRGILLYPELQKTNQVLLQDNDREFKQIFDKIRRLMSDG
                     KRTEMLDTLESLHQRLEAAQQQALQLALAQKPEEALAFVINTFRPLSLALIDKAKRFY
                     EEQSLLLERRKTELAENVRFFNILLLAVSLFAVLVGLTIGIHLSRVITRQLRESIAQL
                     SSSSAEILATTSQVASGAAQTSAAVSQTTATVEEVKQTAQAASQKARYVSESAQKASN
                     ISQAGRKSVEEAIGGMHRIQEQMESIAESIGQLSEQSYAIGEIIATVNDLAEQSNLLA
                     VNAAIEAARAGEQGKSFGVVAQEIRNLAEQSRQATAQVRTILGDIQKATNTAVLAAEQ
                     GNKAVEAGVRQSTETDEAIRLLSTSINEAAQAATQIAASSKQQMVGMDQVAMAMTNIR
                     QASEQNVAGTRQAESAAQGLYQMGRKLSTLVGGRQEANFVERAERVDG"
     gene            1906..4257
                     /locus_tag="VFI43_04500"
     CDS             1906..4257
                     /locus_tag="VFI43_04500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET7061419.1"
                     /translation="MVDKNDALLKRLLAMFRVEAEGYLQDMSAGLLALEAKPGDMKGA
                     SIIETALRSAHSLKGAARAVNLTQIEAACRSLENVFSALKDKSLAISSPLIDLLLQTL
                     DAVGALVAGEDSMMERQKSLLTAITRQLDETMKSPPQGLPRPPRARAASPLSLLLPDE
                     PSPVDAGASANSPSPAPSHASATIRVPTAKLEEVLRQVEELLLPRLAAGQRARELGEA
                     VAAFGEWKKQRQQIRSSIRIIDRELASSSKDGNLQGQHELPRLLEYLEAESLQMKTLE
                     ERLVRLRRETEQDQRALAGMTGSLLHEVKEMQLLPFSTLLDILPRFSRDLAREQGKSL
                     ELVIRGGEMEIDRHILDEMKDTLIHLMRNCIDHGIEQPAARLAKGKPAQGKITLACSQ
                     SDSSTVEILVADDGAGIDVSMLKAMACKLGIMPHEEIEQLDDSELLTLIFRSGVTTSP
                     IITDISGRGLGLAIVREKVEGLGGSINVKSSADTGTTFRILLPLLLANTRGVLVHSGG
                     QLFVIPSAKVERVVRIVNNETQTVENRESVLVDEQLVSLVRLSDTLELPSRSPEVESK
                     SYSVGVVLGSGLGRVAFIVDEILGEQEILVKGLIRPLKRVRNVMGVCMLGTGRVVPVL
                     NVLDLLKSAVKASMSRFNSTPRLSDGKDEASSEQSILVVEDSITARALLKHILESTGF
                     RVTTAVDGVDGYTALKAGAFDLVVSDVEMPRMDGFALTSKIRTDKQLSELPVVLVTAL
                     DSRAHRERGIDVGANAYIVKSDFDQSNLLETIKRLIGVSSIKV"
     gene            4269..5330
                     /gene="cheB"
                     /locus_tag="VFI43_04505"
     CDS             4269..5330
                     /gene="cheB"
                     /locus_tag="VFI43_04505"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HET7061420.1"
                     /translation="MINILVVEDSAVVREFLISIFGSDPDIRVIATAANGEEALEVLR
                     GYRPDVITMDVHMPKLNGLEATRLIMETQPTPIVIVSGSTDPEVATTFDAIDAGAVAV
                     LRRPAGIGHPDHEATARDMIQVVKLMSEVKVIRRWSSMRRGTPTPLPASAPLSRVAQT
                     RIVAIGASTGGPPALETILAALPKHFPAPILIVQHMAAGFTEGFVQWLKNSSNLSVRV
                     ARQCEMPLPGHVYVAPDEYQMKVEKARGIVLTRDEPEHGIRPSVSYLFRSVADTYGRD
                     AVAGLLTGMGRDGADELRVLKDKGAITFVQDKDSSVVHGMAGEAMRLDAATMVLPLEK
                     IAGALTNLVAERGEMKFGS"
     gene            5320..>6338
                     /locus_tag="VFI43_04510"
     CDS             5320..>6338
                     /locus_tag="VFI43_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7061421.1"
                     /translation="MAHDISIHDVRKIEILIAEDSPTQAEKLRYLLEENCYTATTAAN
                     GKEALAAARQRKPTLIISDVMMPEMDGFRLCGEIKKDPELKDVPVILLTSLSDIGDIL
                     KGLESGADNFIRKPYDDEYLLNRIDYLLMSYQLRKSQRMQMGTDIYLGGQKRFISSER
                     QQIVDLLISVYEDAIRINAELSARQLELTHSNRLLTGLYRIAEGLNQAATEREVCENS
                     LEYVMNLPGVKAGWIYLWDGGHLRLAGARNLPASFATNRTEALCECHRLLLAGELDRA
                     PEILKCERIVSREAAASYPSCHASIPLWSGNKCQGIMNLLGFEQYYFKDNELDTFYGV
                     GHQVGV"
BASE COUNT         1598 a         1539 c         1795 g         1406 t
ORIGIN      
        1 gtaccggtac cgtgcacgcc tgcttttgtc cgcgggatcg tcagtgtgcg gggagagatc
       61 ttacctgtca ttgatctgaa gaagtttttc gaactgccgg agaaggaatt gtccgacctc
      121 agtacgctta tcgtactgca catgggagaa ataatgttca gcatcctggc ggacaaaatc
      181 gtaagcgtac gacgtatcgt ggcagccgac atccagcctc ccctacccac acttacgggt
      241 attcgcaaga attatgtaaa ggggataacc tccgagcggc tggtggttct tgacgcggca
      301 aagcttctca tggacgagag cctcgttgta gaggaacaag tgggtgagta gggaagtatc
      361 gcaatagaag ttgaaaaccc tgaatttaag tggcgaagcg aagccttgaa ggaaacatga
      421 gcatcagtaa aaagataatt ggggggtatg gaattgtact tgccctgctt gcactctcga
      481 ccgctgtcgc attctacgca ctcaaccaga ggcaggcagc ctatgagcat ttccttcgcg
      541 tagacgagcg gctgatggaa agcgcaaacg aattacgaat tgctgcgttt gctcaacaaa
      601 cctacatccg gggaatcttg ctgtatcccg agctccagaa gactaaccag gtgctcctgc
      661 aagacaatga tcgcgagttc aagcaaatct tcgacaaaat tcggcgactg atgagtgacg
      721 gcaagcggac tgaaatgctc gataccctcg aatctctgca tcaacgcttg gaggcggctc
      781 agcaacaagc tctccagctc gcgcttgcgc aaaaacctga agaagccctc gccttcgtga
      841 tcaatacgtt ccgcccgctc agccttgcac tgatagataa agccaagcgt ttctatgagg
      901 aacaatccct tcttctagaa cgaagaaaaa ctgaacttgc ggagaacgtg aggtttttta
      961 acatcctatt gctggccgtt tctttattcg ctgttttagt cgggctgaca attggaatcc
     1021 atttaagccg tgtaattact cgacagctta gagagagcat tgctcagctc tcatcgtcgt
     1081 cagcagagat tctcgccacc acctctcagg ttgcctccgg tgcagcccag acttcggctg
     1141 ccgtgagtca aacaactgcc acggtagaag aagtcaaaca aaccgcacag gcggcaagcc
     1201 agaaagccag atatgtttcg gaaagtgcgc aaaaagcttc aaatatctcc caggctgggc
     1261 gtaaatcagt ggaggaagct atcggtggaa tgcatcggat tcaggaacag atggaatcaa
     1321 tagccgagag tattgggcag ctctctgaac agagctatgc cataggcgag attattgcga
     1381 cagtcaacga tcttgctgag caatccaatt tgttggcggt gaacgcagcg attgaggcag
     1441 cccgtgcggg ggaacaaggc aagagttttg gcgtagttgc tcaggagata agaaacctgg
     1501 ctgaacaatc caggcaagcg acagcgcagg tgcgcacgat tctgggcgat attcagaaag
     1561 cgactaatac cgctgtactc gccgcagagc aggggaataa agcagtagag gcgggtgtga
     1621 ggcaatccac tgaaactgat gaagcaatcc gcttgctctc caccagcatt aatgaggcgg
     1681 cgcaagcagc cacacaaatt gcagcctcaa gcaagcagca aatggttgga atggaccagg
     1741 tagcgatggc tatgactaat atcaggcagg cgagtgaaca gaacgtggca ggcaccaggc
     1801 aggctgaatc ggccgcccaa ggcttgtatc agatggggcg caagttgagt acccttgtag
     1861 ggggtcggca agaggccaat tttgtcgaga gagcggaaag ggttgatggt tgacaaaaat
     1921 gatgcgcttt tgaaaaggct ccttgcgatg tttcgcgtgg aggcagaggg gtatctccag
     1981 gacatgtcgg caggactgct tgcgttggag gcaaaacctg gagacatgaa gggcgccagt
     2041 attatcgaga cagcgttgag gtctgcgcac agcttgaaag gcgcggcgcg ggcggtaaac
     2101 cttacgcaaa tcgaagcggc ttgccgatcg ttggaaaacg tgttctcggc actgaaagat
     2161 aaaagccttg ccatatcatc accgctgatc gatctgctgc ttcagacact cgatgccgta
     2221 ggcgcgctag ttgcgggaga ggacagcatg atggagcggc aaaaatctct attgaccgca
     2281 atcacccgac agctggatga aacaatgaaa agtccaccgc aaggattgcc aaggcctccg
     2341 cgggcgcggg ctgcgtcacc tttatctttg cttctccctg atgagccatc gccagtcgac
     2401 gccggcgcaa gcgcgaattc gccgtcccct gctccgagcc atgcatctgc aacaatcagg
     2461 gtgccaaccg caaaactgga agaggttctg cgccaggttg aggaattgct attaccgcgg
     2521 ctggcggcgg gacaacgcgc ccgagaactg ggcgaggccg tcgcggcatt tggcgagtgg
     2581 aagaaacagc gacagcaaat ccggtcgtca attcgcatta ttgatcgtga actggcatcc
     2641 agttccaagg atggaaacct tcaaggacaa catgagctcc ccaggttgct cgaatacctg
     2701 gaagccgagt cgctgcaaat gaaaacactg gaggaacgac tggtgaggtt gcggcgggag
     2761 acagagcagg atcaacgggc actcgccggg atgacgggtt ccctgcttca tgaagtcaag
     2821 gagatgcaat tactgccttt ctcgacgttg ttggatattc tgccccgatt ttcccgcgac
     2881 ctggcccgcg agcagggcaa gagcctggag ctcgtgatcc gtggcgggga gatggaaatc
     2941 gatcggcata tcctggacga aatgaaagat actcttatcc atctgatgcg taattgcatc
     3001 gatcacggga tagagcagcc ggcggcgcgc ttagccaaag gtaagcctgc ccaaggaaaa
     3061 attactttgg catgctcaca aagcgatagc agcacggtcg agatactcgt ggccgacgat
     3121 ggggccggaa ttgacgtctc catgttgaaa gccatggctt gtaagcttgg catcatgcct
     3181 catgaggaga tagagcagct ggatgactcc gaattgctga cgctcatttt tcgatccggg
     3241 gtgaccacta gtcccatcat caccgatatt tccggacgcg gactgggact cgcaatcgta
     3301 cgtgaaaaag tggagggctt gggtggcagc atcaatgtca agtcaagtgc tgatacggga
     3361 acgacgtttc gtatcttact cccgttattg ctggcaaata cccgtggcgt cctggttcat
     3421 tctgggggtc agcttttcgt tattccttca gccaaggttg agcgcgtggt acgtatcgtt
     3481 aacaacgaaa ctcagacggt ggaaaatcga gaatctgttc tggtagatga acaattagtt
     3541 tccctggtcc ggttgagcga cacactggaa ctgccgagcc gttctcccga agtggaatcc
     3601 aagagttatt cggtaggagt ggttctcggt tcgggtctcg gtcgtgttgc atttatcgtg
     3661 gatgagattc tcggcgagca ggaaatcctt gttaaaggac tcatccgccc attgaagcga
     3721 gtaaggaatg tcatgggtgt atgcatgctg gggacaggcc gcgttgtgcc cgtgcttaat
     3781 gtgcttgatc tgttgaaatc ggccgtcaag gcatcgatgt cacggttcaa ctctaccccc
     3841 cgtttatcgg acgggaagga tgaagcttca agcgaacaat ctattttggt ggtggaggac
     3901 tccattacgg cacgcgcact gctcaaacat atactggaat ccacaggctt tcgtgtgacc
     3961 acagctgtgg atggggttga tggctatact gcccttaaag caggggcatt cgacttggta
     4021 gtctccgacg ttgaaatgcc gcgaatggat gggtttgccc taacatccaa gatacgtacc
     4081 gataaacaat tgtcggagct gcccgtggta ttggtaacag cattggactc gcgggctcac
     4141 cgggagcgcg gtatcgatgt cggcgcgaat gcgtacatcg tgaagagcga ctttgatcaa
     4201 agcaacttgc tggaaaccat taaacgcctt atcggcgtca gcagcatcaa ggtctgagtt
     4261 cccgtcccgt gataaacatt ttagtagtgg aagattccgc ggtagtgcga gagtttctca
     4321 tttccatatt cggttcggat cctgacatca gagttattgc gaccgctgct aacggagagg
     4381 aagcactcga agttttgcgc ggctatcgac ccgacgtgat taccatggac gtccatatgc
     4441 cgaagctgaa cggtttggaa gcgacacgac tgattatgga gacacagccc actcccattg
     4501 tcattgttag cggcagtaca gatccagagg ttgcgacaac atttgatgca atcgacgcag
     4561 gcgccgtggc cgtactccgc cgtcccgctg gcattgggca tccggatcat gaggccaccg
     4621 cccgggatat gatacaggtc gtaaaactga tgtcggaagt aaaggtaata cggcgctggt
     4681 caagcatgcg cagagggact ccgactccac ttcctgcaag tgctcctctg agtcgagtgg
     4741 cccagacaag aattgtcgcc attggtgctt caactggcgg tccacctgcg ctcgaaacga
     4801 ttcttgctgc actcccgaag cattttcctg cgcctattct aatcgtccaa cacatggcgg
     4861 caggattcac agaaggattt gttcagtggc taaagaactc gtccaatctt tccgttcgag
     4921 ttgcccgaca gtgcgaaatg ccactaccag gccatgttta tgttgccccc gacgaatacc
     4981 aaatgaaggt agagaaagca agggggatcg ttctgacaag ggatgagcca gagcacggga
     5041 taaggccctc cgtttcatat cttttccgat cagttgccga cacctacggt cgtgatgccg
     5101 ttgctggttt attgacgggc atgggccggg acggtgcaga cgaactgcgc gtactaaagg
     5161 ataaaggggc gattaccttc gtacaggata aggacagttc cgtcgtccac ggaatggcgg
     5221 gcgaggccat gagactggat gcggcaacaa tggtcttgcc cttggaaaag attgccggag
     5281 cgctaacaaa tctagtggct gaaagaggag aaatgaagtt tggctcatga tatttcaatc
     5341 catgacgttc ggaaaataga gattctaatt gcggaggata gtcccaccca agctgaaaaa
     5401 ctaaggtatc tgctggaaga aaattgctat acggctacaa ctgcggccaa cggcaaggag
     5461 gctctggctg cagccaggca gcgtaaaccg accttgatta tcagcgacgt gatgatgccg
     5521 gaaatggacg ggttcaggct atgtggcgaa atcaagaaag accccgagct caaggatgtt
     5581 cccgtgattc tgcttacttc cctgtctgat attggggaca tcctgaaagg attggaatcc
     5641 ggcgccgaca attttatccg caagccgtac gacgatgagt atcttcttaa tcgcattgac
     5701 tatctgctga tgagctacca gttgcgcaag agtcagagga tgcaaatggg cacggatatt
     5761 tatctgggcg ggcaaaaacg tttcatctcc agcgaacggc agcagatcgt cgatttattg
     5821 atttcagtct atgaggatgc cattcgcatc aatgcagaac tgagcgcacg gcaactcgag
     5881 ttgactcact cgaatcgctt gcttaccggg ttgtaccgga ttgcagaagg tctgaaccaa
     5941 gcggcgaccg agcgcgaggt ctgcgaaaat tcgcttgagt acgtgatgaa tttgcccgga
     6001 gtcaaggcgg ggtggattta tctatgggat ggaggccacc tgcgtttggc cggggcgcga
     6061 aacttgcctg catcattcgc taccaaccgg acggaagcac tatgcgaatg ccaccgcctc
     6121 ttgcttgcag gagagctcga tcgcgccccg gaaattttaa agtgcgagcg tatcgtctca
     6181 agggaagcgg cggcttcata cccatcctgc catgccagta taccgttgtg gagcggtaat
     6241 aagtgccagg gcataatgaa cctccttggt tttgaacaat actactttaa agataatgag
     6301 ttagacacat tttacggggt cgggcaccaa gttggtgt
//
LOCUS       DASSHJ010000114        11423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1415525,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000114 DASSHJ010000000
VERSION     DASSHJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415525"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..959
                     /gene="truA"
                     /locus_tag="VFI43_04515"
     CDS             129..959
                     /gene="truA"
                     /locus_tag="VFI43_04515"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7061422.1"
                     /translation="MRIALLLEYDGSGFCGWQSQPSGGSIQDALEAALTKIACEPIRV
                     VTAGRTDAGVHAAYQVVHFETQVQRPMTSWVRGVNAFLPVSVSVLWASQTPDDFHARY
                     CAIERSYLYLLLNHPVRPGLYHGKIGWYHQPLDTDAMRTAAEWLLGEHDFSAFRAAEC
                     QAKSPIRNMTKIGVVRQGDIVAFEFRANAFLHHMVRNIVGCLVYVGKRRYPPEWVGQL
                     LEGRQRREAAPTFPPEGLYLMGIRYDDRWNIPKPAESSFGAVMQRFGRPVDETVTGFS
                     "
     gene            979..1605
                     /locus_tag="VFI43_04520"
     CDS             979..1605
                     /locus_tag="VFI43_04520"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7061423.1"
                     /translation="MTVGAKICGIMRLEDAKAAVHYGADAVGFVFWKGSARYIAPERA
                     REIIAELPPFISSVGVYVDPNATWVEESASVARLSLLQFHGNEEPDFCRRFFLPYIKA
                     VRVRAGLDLLQYATRYTGARGLLMDTYVEGEPGGTGNAFDWDLIPRELPLPVILSGGL
                     QVENVAEAIRRVQPSAVDVSSGVETAKGIKNPQKIAAFMRGVRAGEHL"
     gene            1592..2788
                     /gene="trpB"
                     /locus_tag="VFI43_04525"
     CDS             1592..2788
                     /gene="trpB"
                     /locus_tag="VFI43_04525"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7061424.1"
                     /translation="MNIYDLPDSRGHFGPYGGVFVAETLIAALEDLRREYERYRNDDD
                     FRAEFAYELKHYVGRPSPIYHAKRWSTHLGGAQILLKREDLNHTGAHKINNAMGQALL
                     AKRMGKSRVIAETGAGQHGVASATVAARYGMQCVVYMGSTDVERQAANVYRMKLLGAT
                     VVPVESGSRTLKDALNEAMRDWVTNVEDTFYIIGTVAGPHPYPMMVRDFQAIIGTESI
                     QQMNDEYGQQPDVLIACVGGGSNAIGLFYPYIDTGIRMIGVEAAGLGIESGQHAATLT
                     SGSPGVLHGNRTYLIQDENGQIVETHSISAGLDYPGVGPEHAWLKDSNRAEYVAITDA
                     EALTAFHSLCHYEGIIPALESSHALAYAAQLAPTLDRDKLVLVNLSGRGDKDMTTVAQ
                     ASGIAF"
     gene            2819..3652
                     /gene="trpA"
                     /locus_tag="VFI43_04530"
     CDS             2819..3652
                     /gene="trpA"
                     /locus_tag="VFI43_04530"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7061425.1"
                     /translation="MSRITPVFDKLREQRRKALIPFITAGDPEPAMMVPLMHELVRAG
                     ADIIELGVPFSDPMADGPAIQRSSERALAYRVGLNDVLSMVEAFRKSNGTTPVVLMGY
                     ANPVEAMGYEVFAARAKGCGVDGVLTVDYPPEEAGELVRHLENQQLDAIFLLSPTTAE
                     ARIEKVAQFATGYIYYVSLKGVTGSHRLDLRDVAAKLAEVRSRVSIPIGVGFGIRDKA
                     TAKAVAELADGVVVGSRIVEEIEKSSRGELLANVYQLVSSLRAAVDEAGWEQKSLTEQ
                     C"
     gene            3693..4553
                     /gene="accD"
                     /locus_tag="VFI43_04535"
     CDS             3693..4553
                     /gene="accD"
                     /locus_tag="VFI43_04535"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET7061426.1"
                     /translation="MSWFQKLLPPKIKRKESGEKKAVPEGLWSKCASCDAVLYYSDLG
                     NNLNVCPKCNNHNRISARVRLDLFLDPEGRYEIGPEVQSLDPLKFRDSKRYVDRLAEA
                     KRETDEDDALVVMQGNVKAVPLVAAAFEFGFMGGSMGSVVGERFVRGIQICIEQRLPL
                     VCFAASGGARMQEGLLSLMQMAKTTAALAQLGEERLPFISVLTDPTMGGVSASFAFLG
                     DVVIAEPGALIGFAGPRVIEQTVRQTLPEGFQRSEFLLQHGAIDMIVDRRRMREKIAN
                     LCTLLMHATQ"
     gene            4609..5958
                     /gene="folC"
                     /locus_tag="VFI43_04540"
     CDS             4609..5958
                     /gene="folC"
                     /locus_tag="VFI43_04540"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7061427.1"
                     /translation="MTDSAETLNGLGNVHPPLKPRTLSDWLSFLESLHPKAIEMDLER
                     VDRVRAQLDLAPVFPVITVGGTNGKGSTCAMLEAILGAAGYRTGCYTSPHLLRYNERV
                     RIDGREVSDEMLCGAFEAVEAARQVSKVSLTYFEFGTLAAVELFKKAGVEVAILEVGL
                     GGRLDAVNVFNPDVAVITSIDLDHMDYLGDTREKIGCEKAGIFRSGRPAICAERNVPS
                     IVREYANEIGACFLHIGEHFGYLTNEKGWSYWSEYGKRHALPYPALRGSRQLDNASAC
                     LAVLDAIKDRLPVTMGNIREGLLDVEWPARFQVLPGRPVTVLDVAHNPAAAKVLAESL
                     FRMGRFGKTYGIFAMLRDKDIEGVVRALAGQINIWLTAGIDAPRGASTHETAGAVREA
                     LRENGELVEEAVLTFHDPAAAYAYACERATVNDRICVFGSFHTVAEVLRNRDMVGRG"
     gene            5981..6658
                     /locus_tag="VFI43_04545"
     CDS             5981..6658
                     /locus_tag="VFI43_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7061428.1"
                     /translation="MAKTSEEEIQLRKRARRRLVGAIALVVAAIVFLPMLLDTEPEER
                     EQEIDIRIPPEDSAEEFSHEGASPSGASNDSVSATDSLTTEKPPTGTGQSQQGSDLPV
                     KPMSSAEPAMGKQAEGSRTGKVEAETEKSASPNLASGGFVVQLGAFSSLAKARQQVEI
                     LASRSPGEYTGGGATVYTETFKADKGSGKSEVTRVRVGPFATREQADAALARLKLEGL
                     DGVVTDK"
     gene            6706..7251
                     /locus_tag="VFI43_04550"
     CDS             6706..7251
                     /locus_tag="VFI43_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7061429.1"
                     /translation="MAAFPDILSQMTAFDYCVIAIVGISVLLSVMRGAVRELISLAGW
                     VTAFMVANYFAAPFAPNLPSAIPGESLRMVLAFSILFLLTLLTMGFIAMLASSVTRKA
                     GLGFADRFLGSLFGLARGLLVIMVIVLAGGLTSLPQEPFWRKAVLSAPLESAVTMAIP
                     WLPHDLSRHFVRMKRNNGNFS"
     gene            7383..8903
                     /gene="purF"
                     /locus_tag="VFI43_04555"
     CDS             7383..8903
                     /gene="purF"
                     /locus_tag="VFI43_04555"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7061430.1"
                     /translation="MCGILGLVANAPVNQLLYDGLLVLQHRGQDAAGIVTAEGSTFHM
                     HKGGGLVRDVFRTRNMRSLAGNMGIGHVRYPTTGSAESPAGAQPFYVNSPFGIVLGHN
                     GNLTNAMQLNQELFREDLRHVNTNSDSEVLLNVLAHELQRSTKNHHLDPAAIFTAVAG
                     VHQRCQGAYAVVAMIAGYGLLAFRDPHGIRPLVFGSVERESGVDYLVASESVALDTLG
                     FKLIRDVAPGEAIFIDDRGNFYNKQCAANPSHNPCIFEYVYLARPDSMMDGISVYETR
                     LNMGESLADKITSSMRHLDIDVVIPIPDSGRPCALQLANRLGVSFREGFVKNRYIGRT
                     FIMPGQQQRRKSVRQKLNAMSVEFRGKNVLLVDDSIVRGTTSREIVQMAQEAGANKVY
                     FASAAPPVRFPNVYGIDMPTHEELIATGRDEDEICREIGADYLVYQDLDALVDDVRKL
                     NPSITNFETSCFDGRYITGGITDEYLSVLAAYRHGAAPLSRPASSTQLDLNLVRVE"
     regulatory      8948..9024
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            9060..10250
                     /locus_tag="VFI43_04560"
     CDS             9060..10250
                     /locus_tag="VFI43_04560"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HET7061431.1"
                     /translation="MSDDFELETLALHTGVHRSQFNEHSEGLYLTSSFVFDNAAQAAA
                     RFSGAEAGNIYSRFTNPTVTAFEERLAALEGGEACVATSSGMSAILSCIMGLLSAGDH
                     IVASQSLFGATVNLFNNILKRFNIDTTFVSATDVSAWESAIRPNTRLFFIETPSNPLT
                     EISDIAALAALARKSGAWLAVDNCFCSPMLQRPLEFGADLVIHSATKYLEGQGRVLGG
                     AVVGKRDLLMEGGVFGFLRTAGPTLSPFNAWVILKGMETLKIRMEAHSANAFDVARWL
                     EGHPNVEKVYYPGLPSHPQYELGKRQQKTGGGIVSFEVKDGKEGAWRVIDSVRMLSIT
                     ANLGDVKSTLTHPATTTHARISQESRDAAGISDGLLRIAVGLEAVKDITADLARGLGD
                     ANLS"
     gene            10644..11414
                     /locus_tag="VFI43_04565"
     CDS             10644..11414
                     /locus_tag="VFI43_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_256324148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XDD4 family exosortase-dependent surface
                     protein"
                     /protein_id="HET7061432.1"
                     /translation="MKKFHVLAGLFAMASLDATAAAITFTGAGTGPGGVVVTASAIFD
                     ITGNDLTITLRNTSPSNTGQDVPGSTLTGLFWDFTGNPTLTPVSALLGSGSAFVGAAC
                     DQAGCSGNTNLGGEFGYQATSLTGGADRGISSSGYLTTGVSGNLGNFNGVDLDNPKSL
                     DGINYGIVSAAAGLNLNGGTQSVPLIQDSVVFILHGVTGLSTSDISHVSFQYGTSLTE
                     LNVPGDPRDPPVLIPEPGTLALLGLGLAGLGVLRRRKK"
BASE COUNT         2498 a         2913 c         3380 g         2632 t
ORIGIN      
        1 gcaatactga acaggttgta gggcggggtt ttggttgact cttccgatgc ttgccgggat
       61 ttctgatatc tgccgatgct gacttcaatg attgcctgct cgcgaactgg gatagtctca
      121 gctgggttgt gaggatcgcg ctgttactcg aatatgatgg cagcggtttt tgcggctggc
      181 aaagccagcc atcaggcggt tcgatacagg atgcgcttga ggcggcatta acaaaaatag
      241 cgtgcgaacc cattcgcgtc gttacagcgg gacgaaccga tgcgggcgtc cacgccgcgt
      301 atcaagtcgt tcacttcgaa acacaggttc agcggcccat gacgagttgg gtgcggggcg
      361 tgaatgcgtt cttgcccgtt agcgtttcag ttctttgggc gtcgcagaca ccggacgatt
      421 ttcatgcgcg atattgcgca atcgagcgca gctatctcta cctcttgctg aatcatccgg
      481 ttcggccggg cctctaccac ggcaaaatcg gttggtatca ccagcccctg gatactgacg
      541 caatgcggac agcagcagaa tggctgctgg gcgagcatga tttcagcgca ttccgcgccg
      601 cggagtgtca ggcaaaatct cccatacgga atatgacgaa aatcggggtt gtgcggcagg
      661 gggatatcgt cgctttcgaa ttccgcgcaa atgcttttct gcatcacatg gtacgcaata
      721 ttgtaggttg cctcgtatat gtaggtaagc gcagatatcc cccggaatgg gtggggcagt
      781 tactggaggg gcgtcagcgc cgcgaggccg ctccgacatt tcccccggag ggactatatc
      841 tgatgggtat aagatatgat gaccgctgga acatcccgaa gccggccgag tcttcatttg
      901 gagcggtaat gcaacgcttc ggccggccag ttgatgaaac ggtcactggc ttctcatgat
      961 gcgatgcgaa cgaagtatat gactgtgggg gccaaaatct gcggaattat gcggcttgag
     1021 gatgcaaaag ccgcggtgca ctacggcgcg gatgccgtcg ggtttgtctt ctggaaagga
     1081 agcgcgcgat atatagcacc agaaagggcg cgggaaatca tagccgaact cccaccattc
     1141 atcagctcag tcggtgtata cgtggatccc aatgcaacat gggtcgaaga atcggcctcg
     1201 gttgccagac tcagcctttt gcagtttcat ggcaatgaag agccggattt ttgcagacgg
     1261 tttttcctgc cgtacatcaa agcggtacgc gttcgggcgg ggctggattt gctacaatac
     1321 gcgactcgct ataccggagc caggggcctg ctgatggata cttatgttga aggcgagccc
     1381 ggtggtacgg gcaatgcttt cgactgggat ttgattcccc gggaattgcc attgccggtc
     1441 attctatcgg gcggattgca agttgaaaac gttgcagagg cgatcaggcg ggtccaacct
     1501 tcggcggtag atgtatcgag cggcgtggaa acagccaagg gcatcaaaaa tccgcaaaaa
     1561 attgctgcat tcatgcgagg agttcgagcc ggtgaacatt tatgatcttc ccgacagccg
     1621 gggccacttc ggtccatacg gaggcgtgtt cgttgccgag acgctgattg ctgctctgga
     1681 agacttgcgc cgtgagtacg agcgctatcg caatgacgat gatttccgcg ccgaattcgc
     1741 ctatgagctg aagcattacg tagggcggcc cagccccatc tatcacgcga aaaggtggtc
     1801 gacccatctc ggcggggcgc aaatcctgct gaaacgcgag gacctgaatc acaccggcgc
     1861 tcacaaaatc aataatgcca tgggtcaggc cctgctcgcg aaacggatgg gtaaatcgcg
     1921 tgtaattgcc gaaaccggag cgggccaaca tggcgtggca agcgccaccg tggccgcgcg
     1981 ttacgggatg caatgcgtgg tttacatggg ttccactgat gtggaacgcc aggcggccaa
     2041 tgtttatcgg atgaaacttc tgggagcaac agtggtgccg gtcgaatccg gctcaagaac
     2101 gttgaaagac gcattgaacg aagccatgcg ggactgggtg accaatgtcg aagatacttt
     2161 ttatatcatc ggcacggtag ctggcccgca tccttatcca atgatggtac gggattttca
     2221 ggcaatcata ggaaccgagt ccattcagca gatgaatgat gagtatggcc agcaacccga
     2281 cgtgctgatt gcatgcgttg gcggcgggtc taacgccatc ggactttttt atccatatat
     2341 tgatacaggc atccgcatga taggcgtcga ggcggccggg cttggaatag aaagcggcca
     2401 gcacgctgca accttgacta gcgggagccc gggcgtattg cacggcaatc gcacctatct
     2461 catccaggat gagaacgggc agatcgtcga gacgcattcc atttcagccg ggctggatta
     2521 tccaggggtg ggcccggagc atgcatggct caaggacagc aaccgggcgg aatatgttgc
     2581 aataaccgat gctgaggctt tgacagcctt ccacagtctc tgccattatg aaggcatcat
     2641 acccgctctt gaatccagcc atgcgctcgc atatgcggcg cagcttgcgc ccacactgga
     2701 ccgtgacaaa ctcgtgctcg ttaatctatc cggccggggg gacaaggata tgacaacggt
     2761 agcgcaggct tcaggcatcg ctttttgata gcatttcttc ctgacagaag ttccatccat
     2821 gtctcgtata acccctgtat tcgacaagct ccgggagcag agaagaaaag ctctcatccc
     2881 tttcatcact gccggtgatc ctgaacctgc tatgatggtt cctttgatgc atgaactggt
     2941 cagagcaggg gcagatatca tagagcttgg cgtcccgttt tccgatccga tggccgacgg
     3001 tccggcgata caaaggtctt cagagcgggc actggcatac cgggtgggtt tgaacgatgt
     3061 gctgtcgatg gtggaggcct tccggaagag caacggcacc acgcccgttg tcctgatggg
     3121 ttatgcaaat cctgtagaag cgatgggtta tgaagtgttt gctgcgcgtg ccaaggggtg
     3181 cggagtcgat ggcgttctga ccgtcgatta cccgcccgaa gaagcagggg agttggtaag
     3241 gcatcttgaa aaccagcagc tcgacgcaat tttccttctc tccccgacaa ccgctgaggc
     3301 acgcatcgaa aaggtggcgc agttcgcgac cggctatatt tattatgttt ccttgaaagg
     3361 ggtaaccgga tcgcaccgtc ttgatctgcg tgatgttgcg gccaagctgg ccgaggtgcg
     3421 ttcgcgcgtt tccatcccca ttggcgttgg ttttggcatc cgtgacaagg cgacagccaa
     3481 ggcggtggca gagcttgcgg acggcgtggt agtgggcagc cgcatcgtgg aggaaataga
     3541 aaaatcttcg cgaggggaac ttcttgccaa cgtttatcag ttggtcagca gcttgagagc
     3601 ggcagtcgat gaagcgggct gggagcaaaa gagtctgacg gaacaatgtt gagtcgaacg
     3661 atgtcgagcc ccttcggtaa aggcgagggc ttatgagctg gttccagaaa ttacttccac
     3721 ccaagatcaa gcgcaaggaa agcggcgaaa aaaaggccgt acctgaaggg ctttggagca
     3781 agtgcgcatc gtgcgatgcc gtcttgtatt actcggatct cggaaataat ctcaacgttt
     3841 gccccaagtg taataatcac aacaggattt ccgcccgcgt tcgcctggat ttgtttcttg
     3901 atccggaagg ccgctatgaa atcgggccgg aggtgcagtc gctggaccct ttgaagttca
     3961 gggacagcaa gcgctatgtc gaccgtctgg ccgaggcgaa gcgcgagacc gatgaggatg
     4021 atgcgctcgt ggtgatgcag gggaatgtca aggcagtacc ccttgtggcg gcggcgtttg
     4081 aatttggatt tatgggtggt tccatgggtt cagtggtggg cgagcggttt gtgcggggaa
     4141 tccagatatg tatcgagcag cgcctccctt tagtatgttt tgcagcaagc ggtggcgcgc
     4201 gcatgcagga agggttgctg tcgttgatgc aaatggcaaa gacaactgct gcgcttgccc
     4261 aattgggtga ggaaaggctt ccatttattt ccgttttgac agatcccacc atgggcggtg
     4321 tatccgccag tttcgctttt ctcggcgatg tggtgattgc ggaacccggt gcattgatcg
     4381 ggtttgcggg acctcgagtc atcgagcaga cggtgcggca aacgctgccg gaaggatttc
     4441 aacgttccga attcctgctg cagcacgggg ccatagacat gatcgtggac cgcaggcgta
     4501 tgcgggaaaa aattgccaac ctgtgcacct tgctcatgca cgcgactcag taacaacgcg
     4561 gactgcttca gcaggtcctc gtcgcacttc tttactccat ttcattttgt gactgattcc
     4621 gctgaaaccc tgaacggcct gggcaacgtc catcctcctt taaaacctcg cacgctctct
     4681 gactggttga gtttcctcga aagtcttcac ccgaaggcta tcgagatgga tttggagcgc
     4741 gtagatcgcg tcagggccca gttggatctg gccccggtct ttcccgtgat aaccgtcggg
     4801 ggtacaaacg gcaaaggatc gacatgcgcg atgctcgaag cgattttagg cgcggccgga
     4861 taccgcacag gctgctatac atctcctcac ttacttcgtt acaacgagcg ggtgaggatc
     4921 gatggacggg aagtgagcga tgaaatgctg tgcggagcat ttgaagcagt ggaggcagca
     4981 cgccaggtca gcaaagtttc tttgacctat ttcgagtttg ggacacttgc agcggttgag
     5041 ttattcaaaa aagccggggt tgaggttgca attctggagg tgggacttgg cggacggctg
     5101 gacgcggtca acgtgttcaa tcctgacgtt gcggtgatca ccagcatcga tctcgatcac
     5161 atggattatc tcggcgatac gcgcgaaaag atcgggtgcg agaaggccgg aatcttcaga
     5221 agcggccgac cggcaatatg cgcggaaaga aacgttcctt ccattgtccg cgagtatgca
     5281 aacgagatag gcgcgtgttt tctgcatatc ggggagcact tcggctattt gacaaacgaa
     5341 aaggggtgga gctactggag cgagtacgga aagcgccatg ctcttcccta tccggctcta
     5401 cggggatccc gtcaactcga taacgcgagc gcctgccttg ctgtccttga tgccatcaag
     5461 gatagacttc ccgtcacgat gggcaatatc cgcgagggct tgctggatgt cgaatggcct
     5521 gcgcggtttc aggttttgcc ggggcgtccg gtaacagtgc tcgatgtggc gcacaaccct
     5581 gctgcggcga aagttctggc agaaagtctt tttcggatgg gccgcttcgg caagacctac
     5641 ggcatattcg cgatgcttag ggacaaggat atagaagggg tagtacgggc actggcgggg
     5701 caaataaata tatggctgac cgcaggcatc gatgctcccc gcggcgcttc aacccatgag
     5761 accgcggggg cagtgcgcga agcgctgcgg gagaatggcg agctggtgga ggaggccgtc
     5821 ctgacctttc atgacccggc cgcggcttac gcatacgcct gcgagcgggc cacggtaaat
     5881 gatagaatat gcgtctttgg ctcattccac acagtggcgg aggtgttgag gaaccgcgat
     5941 atggttgggc gaggttgagt ggcggcaaaa cagactgcag atggccaaga cgagcgaaga
     6001 agaaattcag ctcaggaagc gtgccaggag acgattggtt ggcgctatcg ctctcgtggt
     6061 ggcggcaatt gtattccttc cgatgctact tgataccgaa ccggaagagc gcgagcaaga
     6121 gatcgatatt cgtatccctc ctgaagactc tgcagaggaa ttcagtcatg aaggtgcatc
     6181 tcccagcgga gcttcaaacg attctgtttc tgcgacagac tcgcttacaa ctgagaagcc
     6241 ccctactgga accgggcaaa gtcagcaagg ctccgatcta cctgtcaagc ccatgtcttc
     6301 ggcagagcca gccatgggaa aacaggcgga ggggagccga actggaaagg tagaggcaga
     6361 aaccgaaaaa tccgcttcgc ctaaccttgc aagcggtgga tttgtagtac agctcggcgc
     6421 cttttccagc ttggcgaagg ccaggcagca ggtcgaaatt ctcgcatcaa gaagtcctgg
     6481 cgagtacact ggcgggggtg cgacggtgta cacggaaacg tttaaggctg acaaggggtc
     6541 gggcaaaagc gaagtgacgc gggtgcgagt cgggcctttt gccactcgcg agcaagcgga
     6601 tgccgcactt gccaggctga agcttgaagg actggatgga gtggtaaccg acaagtagta
     6661 ctatgattaa gtgttgttgt cttcattacc aataactcgc cggcaatggc tgccttcccg
     6721 gacatccttt cacaaatgac cgctttcgac tactgcgtga ttgcgatcgt tggcatctcg
     6781 gtcttgttga gcgtaatgcg cggagccgtc cgtgaactga tatcgctggc gggttgggtg
     6841 accgcgttca tggtagcgaa ttacttcgcg gcgccgttcg cgccgaatct cccgtcggcc
     6901 attccaggtg agtcgctgcg catggtactc gcattttcca tattgtttct cctgactctg
     6961 ctaacgatgg gttttattgc catgctggca tccagcgtca caaggaaggc agggctggga
     7021 tttgccgacc gttttctcgg atcgctgttc ggccttgccc gcgggttgct ggtgataatg
     7081 gtcatcgtgc ttgcgggagg gctgacgtca ttgccgcagg agccgttctg gcggaaagcg
     7141 gtactgagcg ctccgctcga gagcgcggtc acgatggcga taccttggct gccccacgat
     7201 ctgtccaggc attttgtcag aatgaagcga aacaacggga atttctcctg aagcctgcgc
     7261 agctttattg caagtgtttt tcccccagcg agcgggccac ccgcttcagc ttgcgctttc
     7321 agtgccgtgt cttgcaactt tttgtagctc gctgatctaa gccatctttt acggattgca
     7381 atatgtgtgg aattctcggt ctggttgcaa atgctcccgt caatcaactc ctttatgacg
     7441 gcctgctggt tctccagcat agagggcagg atgccgcggg tatcgtgact gcagaaggca
     7501 gtaccttcca catgcacaag ggaggtggct tggtaaggga tgtgtttcgc acgcggaata
     7561 tgcgttcgct tgccgggaat atgggaattg ggcacgtaag gtaccctacc accggctcag
     7621 ctgaatcacc tgcgggggca cagccatttt atgtgaactc tccgtttgga attgtcctcg
     7681 gacacaatgg caatctcacg aatgcgatgc agctcaacca ggagttgttt cgcgaggatt
     7741 tgcgccatgt caatacaaac tcggattccg aagtgcttct caatgtgttg gcgcacgaac
     7801 ttcaaagaag cacgaaaaac caccatctcg atcctgcagc catcttcaca gcagtagcgg
     7861 gggttcatca gcgctgccag ggtgcctatg ccgtggtcgc catgattgcg ggttacgggc
     7921 tgctcgcgtt tcgcgatccg cacggcattc gccccctggt attcggttcc gtcgagcgcg
     7981 aaagtggagt ggattacctg gtcgcgtcgg aaagcgtggc gctcgatacc ttggggttca
     8041 agctgatacg ggacgtagcg cctggagaag ccattttcat tgatgacaga ggcaattttt
     8101 acaataagca gtgcgctgcg aatccatctc ataacccctg catctttgaa tacgtttatc
     8161 tggctcgccc cgattccatg atggatggaa tatcagtcta cgaaacgcgg cttaatatgg
     8221 gcgagagcct ggctgacaag attacgtcct ccatgcggca ccttgatatc gatgtagtta
     8281 tcccgatccc cgattcgggc cgtccctgcg cgcttcaact cgcgaatcgc cttggggtga
     8341 gcttccggga ggggttcgtg aagaatcgct acattggacg cacattcatc atgccggggc
     8401 agcagcagcg gcgtaaatcg gtacggcaaa aactcaatgc gatgagtgtg gaattccgcg
     8461 gcaagaatgt tctcctcgtg gatgattcaa tcgttcgcgg gactacgagc cgtgaaattg
     8521 tgcagatggc tcaggaggcc ggggctaata aagtctattt cgcttcggcc gcccccccgg
     8581 tccggttccc gaatgtttat ggaatcgata tgccgactca tgaggagctc atcgcgactg
     8641 ggcgcgatga ggacgagatt tgccgcgaaa tcggcgcgga ctatcttgtt taccaggatc
     8701 tcgatgcgct tgtggacgat gtgcggaagc tcaacccatc tataacaaac ttcgaaacgt
     8761 cgtgtttcga cgggcgttac atcacggggg gtatcacgga tgagtacctg tcggtactgg
     8821 ccgcctatcg gcacggcgct gctcctctat cccgtccggc ttccagtacg cagctggatt
     8881 tgaatctcgt gcgggtggaa tagaatcagc attgatcagg atttgacaaa actcatgtaa
     8941 ttaagcaaca tagctttgcg ccgatttgat atcagcttgc gggcgcgtta taaattcggc
     9001 taaagcggaa ggacccgttt tagcgaatgc tgaacgggtt ttttattgag gagcttaaca
     9061 tgtctgatga ttttgagctg gaaacgctgg cgttgcatac gggagtgcat cgcagccaat
     9121 tcaacgaaca ttcggagggg ttgtacctta cctccagctt tgttttcgac aatgctgccc
     9181 aggctgccgc acggttctcc ggtgcggaag ccggtaacat ctattcacgc tttacgaatc
     9241 cgacagtcac cgcttttgag gaacgcctcg ccgcattgga gggcggggaa gcgtgcgtag
     9301 cgacttcttc cggcatgtca gccatcctct cctgcatcat ggggttgctt tcagcaggag
     9361 atcatattgt tgcgtcgcaa agcctgttcg gcgccaccgt aaacctcttc aataacatcc
     9421 tcaagcgctt caatatagac accacgtttg tttcagcaac tgacgtttcc gcgtgggaat
     9481 cggcgatcag gcccaatacc aggcttttct tcattgaaac gccgtcaaat ccccttaccg
     9541 agatttcaga catcgccgca cttgcagccc ttgccaggaa gtcgggcgca tggcttgccg
     9601 ttgataattg cttctgctcg cccatgcttc agcggccact ggagttcggc gccgatctcg
     9661 tcatccactc cgcgaccaag tacctcgaag gccagggaag ggtgctcggc ggtgcagtag
     9721 tcggaaaacg ggacttgctg atggaggggg gagtttttgg gtttttacgg actgccggtc
     9781 ccaccctgag cccgtttaat gcctgggtta tcctgaaggg gatggaaaca ctgaaaatcc
     9841 ggatggaagc gcattccgca aacgctttcg acgttgcacg atggctcgaa ggtcacccga
     9901 acgtcgagaa agtctactat ccgggcctgc cctcccatcc ccagtacgaa ctcggcaagc
     9961 gccagcagaa gacaggggga ggaatcgttt ctttcgaagt aaaggatgga aaagaaggcg
    10021 cctggcgagt catcgattcg gtccggatgc tttcgatcac cgcgaatctg ggcgatgtga
    10081 aaagcacgct gactcacccg gcgacgacaa cgcacgcccg tatcagccag gagagccggg
    10141 acgcggccgg aatcagcgat ggcttgttgc gcattgcggt cgggctggaa gcggtaaagg
    10201 acattactgc agacctcgca agaggcctcg gggatgcgaa tctttcctga ttcgaaagat
    10261 caatcgcagg ctggcgagcg gtgctgagcg cgggagcagg tcagcgattg acggagcctc
    10321 gaaactcctt gaatctcttt ccgcgcgtag cgagagtcgc acaagaatgg gctgttgctc
    10381 gactagcatc tttttggcgt ttgcggcccg ctttatgaat tgacgtacgg tgcgagcgct
    10441 gatccccgac ttggcactag gaccccgaga ttttaattta acactgtcga attttcttac
    10501 atacctctaa cacagcgata actctacaat tgtaatcctt tgtaagagaa ccataacttg
    10561 atcctggcat gggacttgct ttagttctat tagaagtcga gtcgtattaa aaaagaacaa
    10621 ttctttgaca acaagaggag aaaatgaaga agtttcatgt gttagccggc ctgtttgcca
    10681 tggcctcact agatgccaca gcagcagcca ttaccttcac gggagcagga accggtccgg
    10741 ggggagtcgt ggttaccgcg tcagccattt ttgatattac cggcaatgac ctgacaatca
    10801 ccttgcggaa tacgtcgccc tccaatacgg gtcaggatgt gccgggcagc acccttaccg
    10861 gccttttctg ggattttacc gggaatccta ccttaacgcc ggtctcggca cttcttgggt
    10921 cagggtcagc ttttgtgggt gccgcctgtg accaggccgg gtgcagcggt aatacgaatc
    10981 ttggcggcga gtttggttat caggcaacat cgcttacggg gggcgctgac cgcggcattt
    11041 caagctcagg ttacctgacg actggtgtgt ccggcaatct tgggaatttc aatggtgtcg
    11101 atctggacaa tccgaaatct ctcgatggta tcaattacgg tattgtttca gcggcagccg
    11161 gcctgaatct caacgggggg acgcaaagtg tccccctgat ccaggactcg gtggtgttta
    11221 tccttcacgg cgtaactggc ctctcgacca gcgatatttc gcacgtcagt ttccaatatg
    11281 gaacgtccct gacggagctt aacgttcctg gtgatcccag ggatccccct gtcctgatcc
    11341 cggaaccggg tacgcttgcc cttcttggtc ttggcctggc cggcttgggt gttctgcgca
    11401 gacgtaaaaa ataattctca ggc
//
LOCUS       DASSHJ010000115        16089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1040827,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000115 DASSHJ010000000
VERSION     DASSHJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 16089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16089
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040827"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFI43_04570"
     CDS             <1..505
                     /locus_tag="VFI43_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7061433.1"
                     /translation="EIEMNRLLRQRHRLAEGTENDFTVRNLAALASVATGAAKVMSLM
                     LGAIASISLLVGGIGIMNIMLVSVTERTREIGIRMAIGANRRAILAQFLLEALVICIL
                     GGLIGVALGIGGAWAVSRVADMIVVITFGMVALAFAFASAVGIFFGFYPAKKAAALKP
                     VEALRYE"
     gene            781..1653
                     /locus_tag="VFI43_04575"
     CDS             781..1653
                     /locus_tag="VFI43_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061434.1"
                     /translation="MQASPDQTGSFSHLKLDDLLPVFQDKATRVLSSFEKYHPISHDA
                     WEHGKGGKDIMLGYSYTAGCQGHERYVAIRAISFEEFEKDDGLVPKVTAHIHEMEASC
                     GPQLDHYYLLLCTDARKHGEMIRALEQQLRKERAAVVVEPSHAWSFYTLEDTVTDAVS
                     DRLIYANDYVRRQAREQIADIDKRQLILLLSCLVHAIEEMKNFSVADEFVLHNDDVHD
                     FEATHPENYSPLEDMTAMEGRFFFRDADVEGFRIYQDSASAIIALYYDARVRYGHEGD
                     EAVRYLFAFLERTI"
     gene            complement(1870..3717)
                     /gene="glmS"
                     /locus_tag="VFI43_04580"
     CDS             complement(1870..3717)
                     /gene="glmS"
                     /locus_tag="VFI43_04580"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7061435.1"
                     /translation="MCGIVGAVASSNIVPTLLEGLRRLEYRGYDSAGIAVNNGHLHRL
                     RTIGRVAELCKLTDDHKFTGATGIAHTRWATHGAPSERNAHPHFSGTDPKVAVVHNGI
                     IENHESLRQRLQDAGFIFSSDTDTEVIAHLITLKLRETPDLFKAVCLSLQELQGAYAI
                     AVMEEARPERIIVCRNGAPLLLGLGDSGVYAASDASALLQVTRQMIYLEDGDVAELTQ
                     EGYRIVNCPGDGKITEVTRPIQKSELTNESVELGPYAHFMQKEIFEQPGVVANTLEMV
                     VNARSISPGLFGSNGEGIFRDTRNILILACGTSYHAGLVARYWLETVAGVSCNVEIAS
                     EYRYRRPVVHPGTLVVGISQSGETADTLAALSYAKSLGHRYSLGICNVAESALVRQTD
                     LRFLTRAGPEIGVASTKAFTTQLAALMLLTMTMARLNGKSSEQAERQMIKAMRHLPVA
                     LQHALQVEPQVKAWAERFYDKKHALFLGRGMHYPIALEGALKLKEISYIHAEAYAAGE
                     LKHGPLALVDKDMPVVAIAPNDALLEKLKSNLQEVRARGGELYVFADADSQIQESEGV
                     HIIRLAEHAGLLSPILHTIPLQLLAYHVALQKGTDVDKPRNLAKSVTVE"
     gene            complement(3794..5179)
                     /gene="glmU"
                     /locus_tag="VFI43_04585"
     CDS             complement(3794..5179)
                     /gene="glmU"
                     /locus_tag="VFI43_04585"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7061436.1"
                     /translation="MNVPELNIAILAAGLGKRMHSDQPKVLHRLAGKSLLVHVLETAR
                     ALSPARICIIFGHGGEAVPKAIGDPSLTWVKQVPQLGTGHALMQALPYLGSEGATLVL
                     YGDVPLIGLPTLERLVAIAGNDTLALLTVELGDPFGYGRIVRNAAGNIAAVIEEKDAS
                     NSERSIREVYTGITVIPNRYLHSWLHKLENNNAQKEYYLTDIVAMAVRDNVKVVAGHP
                     DRAWEIAGINSKLQLAELERIYQKECASTLLEQGVTLADPSRIDVRGKLTCGADVEID
                     IGCIFEGRVDLADRVKVGAHSILRDVKIAAGSAIAPFSLIESTEVGENCRVGPYARIR
                     PGTRLDNDVHIGNFVEVKNSAIEAGSKANHLSYIGDTVIGKRVNIGAGTITCNYDGAN
                     KHQTIIEDDVFVGSDTQLVAPVKVSKGSTIGAGSTITKDTPPETLTLSRARQTSIDGW
                     RRPVKKAKGEK"
     gene            complement(5339..6592)
                     /locus_tag="VFI43_04590"
     CDS             complement(5339..6592)
                     /locus_tag="VFI43_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061437.1"
                     /translation="MTTSSDSLLFLRWFLMSVVTGIVIAVAVVVAIDPYDQYRVVVKE
                     GFNRVKPELTRYQNEIKLTHAAELRADALILGNSRAEAGLDPEAGAFTQSGLSAYNLA
                     VPGSGILNSRRQAEYLYQIGAKPKTIILGMEFLDFMESGKAAAPAISHTPSIGQVPHP
                     DSWFWRFDSLFSLASLKDAMQTLSIQGDEEADVMTPRGFNPLRGYVPIARREGYFRLF
                     KQRAGENTRIYLRKAKGSLSLDDLVHLNAILDLAAESGTEANLIIYPYHAQILALFEE
                     TGLANAFEEWKMALISEVSAARARHPDARITLFDFSGYGPYNCERIPEHGDHVTSTQW
                     YWEAGHFKKQLGDILLKSVFSSPLLTEQTEGQRPPGMFGFQLDDSNSAENLKRISLER
                     ARCSQSYPELFAEATALVRLERGRN"
     gene            complement(6603..8150)
                     /locus_tag="VFI43_04595"
     CDS             complement(6603..8150)
                     /locus_tag="VFI43_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET7061438.1"
                     /translation="MLFNSFAFIFAYLPAALVGFFWLGSRNHSWAAGWLALMSLFFYG
                     YWDYRYVPLLLASIGFNYWVGLNIALADKPQRKTWLVCAITINLILLAYFKYANFFLD
                     TINFLGNKTFPALDIILPVGISFYTFTQIAFLVDTYEGRVAEYRFLHYLLFVTYFPHL
                     IAGPVLHHREMMPQFAEPGIYRFSASDFAVGLTIFCIGLAKKVLIADNLSPYAGSLFD
                     NPDSPSLLIAWAGVLAYTFQLYFDFSAYSDMAIGLSRLFGIKLPLNFNSPYKASNISE
                     FWRRWHMTLSRFLRDYLYFPLGGNRAGLLNRYRNLTTVMLLGGMWHGSGWNFIIWGGL
                     HGVYLVMHHAWAAVSKRLGFPSDSRTWKLIATAITFTAVCFAWVFFRSPDLATSMEIV
                     KGMTGFYGVGLPDAIGSRVGSFASTLEQLGVHFYLGGGSRFIESWGWIAFAAMLAFLM
                     PNTQELTHLFDPALDFKPHLRESADRLPDWLSWQPSRQWALFLAALAIASLLSLNRPN
                     EFLYFQF"
     gene            complement(8684..9103)
                     /locus_tag="VFI43_04600"
     CDS             complement(8684..9103)
                     /locus_tag="VFI43_04600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET7061439.1"
                     /translation="MGVFHVDIVSAEESIYSGPAEFLVAPAEGGEVGVYPQHTPMLTR
                     IKPGSVRIKAPLKEEELVYVSGGMLEIQPDVVTILADTAMRGADLDEMKAMEAKKRAE
                     DAMKDRSSAMDYARAQAELSEAIAQLSAIQKLRKRGH"
     gene            complement(9187..10566)
                     /gene="atpD"
                     /locus_tag="VFI43_04605"
     CDS             complement(9187..10566)
                     /gene="atpD"
                     /locus_tag="VFI43_04605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7061440.1"
                     /translation="MNEGKIVQCIGAVIDVEFTREGMPKVFDALVLEGSELTLEVQQQ
                     LGDGVVRTIALGSSDGLRRGMMVKNTGDQIRVPVGTKTLGRIMDVLGRPIDEMGPVDA
                     EKTMSIHRKAPDFDELSASTELLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVALVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGR
                     LQERITSTKSGSITSIQAVYVPADDLTDPSPATTFGHLDATVVLSRDIASLGIYPAVD
                     PLDSTSRQLDPLVVGEEHYNTARAVQQTLQRYKELRDIIAILGMDELSPEDKLAVARA
                     RKIQRFLSQPFNVAEVFTGSPGKYVPLKETIKGFKGIVAGDYDNLPEQAFYMVGSIDE
                     AVEKAKTLQ"
     gene            complement(10594..11475)
                     /gene="atpG"
                     /locus_tag="VFI43_04610"
     CDS             complement(10594..11475)
                     /gene="atpG"
                     /locus_tag="VFI43_04610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET7061441.1"
                     /translation="MAGSREIRNKIKSVKNTQKITRAMEMVAASKMRKAQERMRKARP
                     YGDKIRNVAAHMSRAYTEYRHPFLVERDTVKRIGIVVVTSDKGLCGGLNTNVLRMAVN
                     KMKSWEAEGEQIEVCCIGNKGFGFMNRIGANVISHVTGLGDTPDLEKLIGAVKVLLDG
                     YTQDRFDRVYIFYTRFVNTMKQEPVMEQLLPLSDERLKVGEGPKGQRGVWDYIYEPEA
                     RPVIDDIMVRYVEALIYQAVTENMASEQSARMVAMKAASDNAGNVIDELTLIYNKSRQ
                     AAITKELSEIVGGAAAV"
     gene            complement(11479..13020)
                     /gene="atpA"
                     /locus_tag="VFI43_04615"
     CDS             complement(11479..13020)
                     /gene="atpA"
                     /locus_tag="VFI43_04615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7061442.1"
                     /translation="MHLNPSEISELIKSRIEGLGGTSEIRTQGTIVSVTDGIVRIHGL
                     SDVMQGEMLEFPGNTYGLALNLERDSVGAVVLGEYEHITEGDTVKCTGRILEVPVGEE
                     LIGRVVNALGQPIDGKGPVGSKRSEPIEKIAPGVVWRQSVNQPVQTGLKSIDSMVPIG
                     RGQRELIIGDRQTGKTAVAIDAIINQKGENMTCIYVAIGQKASSISNVVRKLEEHGAM
                     EYTIVVAATASESAAMQFIAPYSGCTMGEYFRDSGRDALIVYDDLTKQAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNAAYVEKATNGAVKGKTGSLTALPVIETQA
                     GDVTAFVPTNVISITDGQIFLESDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLERGKMVTELMKQPQYGTLSVPEMALTLFA
                     VNKGYLDDVEVKRALAFESALRSFMRSKYSAIMDKIATTKDLDPATEKDLDAAVQDFK
                     KNGTY"
     gene            complement(13035..13571)
                     /locus_tag="VFI43_04620"
     CDS             complement(13035..13571)
                     /locus_tag="VFI43_04620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET7061443.1"
                     /translation="MAEARTVARPYAEAVFKLAKAKRELAAWSDMLQLAATVASDSNI
                     RALIGDPKISPKRLGELVLEICGAGLSDEGRNFILLLAENGRIDILPEVGELFEQLRT
                     QYEGVMDAKIISAFPLSDDQLKQLVADLEDKFKRKIEPEVRVDRELIGGVKVEIGDEV
                     LDASVRGKLEAMAIALKT"
     gene            complement(13575..14045)
                     /locus_tag="VFI43_04625"
     CDS             complement(13575..14045)
                     /locus_tag="VFI43_04625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET7061444.1"
                     /translation="MNINFTLFSQAFAFAIFIWFTVRFVWPPLMRAIENRQKTIADGL
                     AAGERGKHELELASQRSGDVVREAKQRASDIIAQAEKRAAEIVDEAKNTAKEEGSRIL
                     VGAKADVEQEVFRAREALRQHVAELAVAGASKILRREVDAKAHADLLASIKAEL"
     gene            complement(14076..14348)
                     /gene="atpE"
                     /locus_tag="VFI43_04630"
     CDS             complement(14076..14348)
                     /gene="atpE"
                     /locus_tag="VFI43_04630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET7061445.1"
                     /translation="MDNLQFLAMIQAYTGIGIGLMIGLGAAGACIGIGIMCSSFLDGA
                     ARQPEMIPTLQGKVFLLLGLIDASFIIGVGLAMLFAFGNPLLAVIK"
     gene            complement(14403..15209)
                     /gene="atpB"
                     /locus_tag="VFI43_04635"
     CDS             complement(14403..15209)
                     /gene="atpB"
                     /locus_tag="VFI43_04635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET7061446.1"
                     /translation="MASGSEVTPTSYMAHHLTNLTTSVGEGGFWTLHVDTLVMAVLTG
                     LVSFGFLWWVVRGATSGVPGKRQAFVELAVEFIDEQVKSTFYGDRHAFVAPAALTVFV
                     WVFMMNAMDFLPIDIMAWIYAKLGLHNWRSVPTADVNTTFALALSVWLLMIFFNVKVK
                     GLGGWIHELVCTPFGKNPILWPVNILFNLVEYVSKPLSHSLRLYGNIYAGEIIFLLLG
                     MWAATGLSGTLFGTVLGAGWAIFHILIVTLQAFIFMMLTVVYLSMAHESH"
     gene            complement(15231..15584)
                     /locus_tag="VFI43_04640"
     CDS             complement(15231..15584)
                     /locus_tag="VFI43_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HET7061447.1"
                     /translation="MRWQFIATLAMVLVLGLISGLHGAASALLGGVVSMISAAAFAAI
                     ISRYRGSTAGGVLITAIKAEAAKVFVMVTLLWLVMSFYKDVVAVGFIGTFVLTVIIFG
                     MALFVTDDAKIARNR"
BASE COUNT         3834 a         4527 c         4053 g         3675 t
ORIGIN      
        1 ggaaatcgaa atgaacagac tgttgcggca gcggcaccgc cttgcggagg gtacggagaa
       61 cgactttacc gtgcgcaatc tggcggcact cgcctccgtt gcaaccggcg ctgccaaggt
      121 catgtcgctc atgctcggcg cgatcgcctc gatatcactg cttgtaggcg gtatcggcat
      181 catgaacatc atgctggtat ctgttaccga gcgtacgcgc gagattggca ttcgcatggc
      241 gatcggtgca aaccggcgcg caattcttgc gcaattcctg ctggaagcac tcgtcatctg
      301 catcctggga ggcctcatag gagttgcact gggtattggt ggagcttggg ccgtaagccg
      361 cgtggcagat atgattgttg tcatcacgtt tggcatggtc gccctggcgt tcgcgtttgc
      421 ctcggcagtc ggcatttttt ttgggtttta cccagcaaaa aaagcggcgg cgttgaagcc
      481 ggttgaagcg ctgcgctacg aataggaccg aagagaaagg ccaggcctgc cctggccgcc
      541 aagtaggcga tctcagcagc aggattgcca ggctcagctt gatgtttacc gcaaggcgga
      601 tatggcagac accggtcagg tgaactaccc ttaatctgaa agtgttcagc tgctaaatag
      661 ctacctcttg ttcatgcgcg attcgaagcg tcttttagag cgtgcttaac ggtcaagtca
      721 agaatgattc cacataatgc agtgacacca gatacagttc tccaagagct ggttcgttat
      781 atgcaggcga gccctgatca aaccggctcg ttttcgcatc tcaaacttga cgacttgctc
      841 ccggtcttcc aggataaagc gacacgcgtg ctttcctctt ttgaaaaata tcacccgatc
      901 agccatgacg cctgggagca tgggaaagga ggaaaggaca taatgcttgg atattcatat
      961 acagcaggtt gtcaaggcca tgagcgctat gtcgcgatcc gagccatatc ttttgaggag
     1021 tttgaaaaag atgatggcct tgtacccaag gtcacggctc atattcatga aatggaggcg
     1081 agctgcggcc cccagctgga tcattattac ttgttgctgt gcacggacgc ccgaaagcat
     1141 ggcgagatga tccgggctct tgagcagcaa ctgcggaagg aacgggcggc agtagtggtc
     1201 gaaccctctc atgcctggtc tttttatacc ctggaagata cagtaaccga cgcggtatca
     1261 gacaggctga tctatgcaaa cgactacgta cgacgccagg cccgcgaaca gatagcggat
     1321 atcgacaaac gtcaactcat cctgctgctg tcatgcctgg tccatgcaat cgaagaaatg
     1381 aagaattttt cggttgctga cgagtttgtt ctacacaatg acgacgtgca tgacttcgaa
     1441 gcgactcatc cagagaatta ttctccgttg gaagacatga ctgcaatgga aggccggttc
     1501 ttttttcggg atgccgatgt ggaaggattt agaatctacc aggacagcgc atccgcgatc
     1561 attgccctgt attatgacgc gagagtccga tatggccacg aaggagacga ggccgtgcga
     1621 tatttgttcg cttttcttga gcgaacgatc tgaagcagag cgctgatgaa ggagctttcg
     1681 cccaagcgct acaggttcga gggaactacc tcaatggaac ggcagagctt gtagacagcg
     1741 atattccaaa gattgcggcg aacaaccgcg gcagcgtatt gattaccgaa tcatgatcca
     1801 cgtttcacca ttatgctgac agccctgcag cgaaccctgc cgctctccgg attgccccac
     1861 ctccgacgat tactccaccg tcaccgattt ggccagattc ctgggtttat ccacgtctgt
     1921 acccttttgc agcgcaacat ggtaggcaag caattgcaga ggaatggtgt gaagaatggg
     1981 gctgagcagt cctgcgtgct ctgcaaggcg tatgatatgc accccttcgc tttcctgaat
     2041 ctgcgaatcc gcatcggcga acacataaag ctccccgccg cgcgcacgga cttcctgcag
     2101 atttgatttg agtttttcga gcaatgcgtc attgggagca atggctacca caggcatatc
     2161 cttgtccacc aaggcaagcg ggccgtgctt caactcgcct gcggcataag cttcggcatg
     2221 aatgtacgaa atttccttga gcttgagcgc gccctccagg gcaatgggat aatgcatgcc
     2281 gcgccccaaa aacagtgcat gcttcttgtc ataaaatcgc tcagcccacg cttttacctg
     2341 cggttcgacc tgtaacgcat gctgcagcgc aactggcaaa tggcgcatgg ccttgatcat
     2401 ctggcgctcg gcctgctcag aggatttacc gttgagacgc gccattgtca tggtaagcag
     2461 catgagtgcg gccagctgcg tcgtgaacgc cttggtggag gcaacgccga tctcagggcc
     2521 agcacgggta aggaacctca agtccgtttg tcgcaccaac gcgctttcgg ccacattgca
     2581 gatccccagg ctatagcggt gacccagcga ttttgcataa ctgagggcag caagggtatc
     2641 agcggtttct cctgattgcg agataccgac gaccagggtg ccgggatgca ccaccgggcg
     2701 gcggtaacgg tattcgctcg caatttcgac attgcaggac accccggcga ccgtttccag
     2761 ccagtagcgc gccaccaggc ccgcgtgata gcttgtgccg caagcgagta ttagaatgtt
     2821 gcgcgtatcg cgaaaaatgc cttcgccatt gctgccgaaa agtcccggtg atatggagcg
     2881 ggcatttacc accatctcga gcgtatttgc gacaacgccc ggttgctcaa aaatttcctt
     2941 ctgcatgaag tgagcataag gtcccagctc tacggactca ttggtaagct cgctcttttg
     3001 aatagggcga gtcacttcgg tgatttttcc atcacctggg caattgacaa tgcgatagcc
     3061 ttcctgcgta agctcagcta catcgccatc ctcgaggtag atcatctgcc gggtgacctg
     3121 tagcagggca gatgcgtccg aagcagcgta aactcctgag tcgcccagtc cgagcagcaa
     3181 gggggcgccg tttcggcaaa cgatgatccg ctccgggcgt gcttcttcca ttaccgcaat
     3241 ggcataagcc ccctgcaatt cctgcaggga aaggcacacg gccttgaaca aatccggggt
     3301 ctcccggagt tttagcgtga tgaggtgggc gattacctcg gtgtcagtat cagagctgaa
     3361 aataaagccg gcatcctgca gacgctggcg caggctctca tgattttcga tgatcccgtt
     3421 atgcacgacg gcaactttgg gatcggttcc ggagaagtgt ggatgagcat tacgctcaga
     3481 aggcgctcca tgagtggccc agcgcgtatg cgcaatgcct gttgcaccag taaatttatg
     3541 gtcatccgtg agcttgcaga gctcggcaac cctccctatc gtccgcagcc gatgcaggtg
     3601 gccattattt acagcaatgc ccgcagaatc atagccccga tattcaagac ggcgaagtcc
     3661 ctccaggaga gtgggaacaa tgttgctgct tgctaccgcg ccgactatgc cgcacatacg
     3721 cgattgcctt tttgacccga gcgatgcaac gaaaacgatc gaaaaaccga atcattcaaa
     3781 caagcataga gcttcatttt tcccccttcg cttttttaac cggccgtctc cacccgtcta
     3841 tgctcgtctg tctggcacgc gatagcgtca atgtttctgg aggcgtatcc ttcgtaattg
     3901 tcgaccctgc cccgattgtc gatcctttcg acaccttcac gggggcaacg agctgggtgt
     3961 ccgagcctac aaagacatca tcttcaatga tggtctgatg cttgttcgcc ccgtcgtagt
     4021 tgcaagtgat ggttccggcg cctatgttca cgcgcttccc gatcactgta tctcctatgt
     4081 agctcaggtg gtttgccttg ctacctgcct cgatggcgct attcttgact tccacgaaat
     4141 tgccgatatg gacgtcattg tcgagccttg ttcctggccg gatacgcgcg tacggcccca
     4201 ctcggcagtt ttcgcccact tcagtggact cgataaggct gaatggcgca atcgccgagc
     4261 ccgctgcaat cttgacatcc cttagaatgc tgtgagcgcc cactttcacg cggtcagcca
     4321 aatccacccg cccctcaaaa atgcagccaa tgtcgatctc cacgtctgca ccgcatgtca
     4381 atttgcctcg aacatcgatg cgcgagggat cagccagcgt tacgccctgc tcaagcaacg
     4441 tactcgcaca ttccttttga tagatgcgtt caagctccgc gagctgcaac ttgctattaa
     4501 tacctgctat ttcccaggct cgatcggggt gtccggcgac aaccttcaca ttatccctga
     4561 ccgccatggc aacgatatca gtgaggtagt attccttttg agcgttgttg ttttcgagct
     4621 tatgcagcca actgtggaga taccggtttg ggatgacggt gatgcctgta tagacttcac
     4681 gtatgctacg ctccgagttg cttgcatctt tctcctcgat gacggcggcg atattgccag
     4741 cggcgttacg aacaattctt ccatagccaa acggatcgcc caactcgacg gtcaacaagg
     4801 cgagcgtgtc gttgcccgcg attgccacga gtctttcgag cgtgggaagc cctatcaggg
     4861 ggacatcccc atatagcacc aaggtcgccc cctcgctgcc caggtaaggc aatgcctgca
     4921 tcagggcgtg accggtaccc agctggggca cctgcttgac ccaggtaagc gatgggtcac
     4981 caattgcctt cggtaccgcc tctcccccgt gtccaaatat gatgcaaatc ctggcgggcg
     5041 acagcgcccg cgcggtttcc aggacgtgaa caagaaggga tttgcccgcc aagcgatgga
     5101 gtaccttggg ttggtcggag tgcatgcgct tgcccagccc ggcagcgagt atagcgatat
     5161 ttaactcagg aacattcata tagaaaaatg caattccccc ttagaattgt gaggcttgca
     5221 acgcattcgt ggaacaacga gcgtagcgag gttaaagata acataatgta ctcagcagcg
     5281 gggacttaag cgcctaaaag cagtcgcaat gttcgactac actccttgcg cacgtttctt
     5341 aattacgacc gcgttcgagt cgcacaagcg ccgttgcttc cgcaaacagt tccggataac
     5401 tctggctgca ccgcgcacgt tccaggctga tgcgttttaa attctcggcg ctgttcgaat
     5461 catccagttg aaatccaaac attcccggcg gtctttgccc ttctgtctgc tcggttaaga
     5521 gtgggctgga aaatacggac ttcagcaaga tgtcgccgag ttgcttttta aaatgcccag
     5581 cttcccagta ccactgggtg gaagtcacat gatcgccatg ttccggtatc ctttcgcaat
     5641 tgtaagggcc ataaccgctg aaatcgaaca gggtaatcct tgcatctgga tgtcgcgctc
     5701 gggcggccga tacttcactt attaacgcca ttttccattc ctcaaatgca ttcgccaatc
     5761 ccgtttcctc gaagagagcc aggatttggg catggtatgg gtagatgatc agatttgcct
     5821 cggtgccgga ttcggccgcc aagtcaagga tggcgttaag atgcacgaga tcatcaagag
     5881 aaagcgaacc ctttgctttt cttaaataaa tcctcgtatt ttctcctgct ctttgcttaa
     5941 ataacctgaa ataaccttca cgacgagcaa tcggcacgta tccccttaat gggttgaacc
     6001 cgcgaggggt catcacgtcc gcttcttcgt cgccctggat ggataacgtc tgcatggcgt
     6061 cctttaagga ggccagcgaa aaaagactgt caaaccgcca gaaccaggag tcgggatgtg
     6121 ggacctgacc aatagaaggt gtatgcgaaa tggcaggggc ggccgctttc cctgattcca
     6181 tgaagtccag gaactccatt cccaggatga tggtttttgg cttggcgcca atttgataga
     6241 gatattcggc ctgccgacgt gagttgagga tacccgaacc gggtacggca aggttataag
     6301 cggacaagcc ggattgggtg aaggcccctg cttcgggatc cagccccgct tcagcccggg
     6361 aatttccgag gatgagtgcg tcggcgcgca gttccgctgc atgggtcagc ttgatttcat
     6421 tctggtaacg cgtcaactct ggtttaaccc gattgaaccc ctccttcacc acgacacgat
     6481 actgatcata aggatctatc gccacgacga ccgcgaccgc gatgactata ccggtgacca
     6541 cagacatcag gaaccaccgc aggaagagca gcgagtcgga tgatgttgtc attcctgccc
     6601 gattagaact ggaaatatag aaactcatta gggcggttca gggagagcag gctggctatg
     6661 gcgagagcgg ctagaaacag ggcccattgt cgcgacggtt gccagcttag ccaatccggg
     6721 aggcggtcgg ctgattcccg gagatgcggc ttaaagtcga gggccggatc aaacaggtga
     6781 gtgagttcct gcgtgttcgg cataagaaag gctagcatgg ctgcaaatgc gatccatccc
     6841 cagctttcaa tgaaccgcga tccaccgccg agataaaagt ggacgcccaa ttgttcaagc
     6901 gtagacgcga acgagccaac gcgacttcct atggcatcag gtaatcccac tccataaaac
     6961 cctgtcatgc ctttgactat ttccatggag gtcgcaaggt ctggcgaacg gaagaatacc
     7021 catgcgaagc agacagcggt aaacgttatt gcagtggcaa tcaacttcca ggtacgcgaa
     7081 tccgagggaa agcccagtct ttttgacacc gctgcccatg catgatgcat gacgaggtag
     7141 acgccgtgga gtccgcccca gataatgaaa ttccaccccg aaccgtgcca cattcccccg
     7201 agaagcatga cggtcgtcag attgcgatac cgatttaata aaccagcccg gttccctccc
     7261 aggggaaaat acaagtagtc ccgtagaaag cgggataatg tcatatgcca ccttcgccag
     7321 aactcactga tattggaagc cttgtatggc gaattgaaat tgagggggag ctttattccg
     7381 aagagacgag acaggccgat agccatatcg gagtaagcgg aaaaatcgaa ataaagctga
     7441 aaggtatagg ctagcacccc tgcccatgcg ataaggagtg aaggcgagtc agggttgtcg
     7501 aacagagaac cggcatacgg ggacagattg tcagcaataa gtaccttctt tgccaggccg
     7561 atacagaata tggttaaccc tacagcgaaa tccgaagcgg aaaatcgata aattccgggt
     7621 tccgcgaact gcggcatcat ttccctgtga tgcagcacag ggcccgcgat cagatgcgga
     7681 aaataggtaa cgaacagtaa ataatgaagg aagcggtatt ccgctacgcg tccctcgtag
     7741 gtatcgacga ggaaagcgat ttgcgtgaag gtatagaacg atatgccaac ggggagaatg
     7801 atatccagtg ccggaaaagt tttgtttccg agaaaattga tggtatcgag aaaaaaattt
     7861 gcatacttga aataagcgag caggattagg ttgatggtaa tcgcgcagac aagccaggtc
     7921 tttcgctgcg gcttgtctgc cagcgcaata ttcagcccga cccagtaatt gaagccgatc
     7981 gatgcaagta gcaaaggaac atatctgtaa tcccagtatc cgtaaaaaaa cagcgacatc
     8041 agcgcgagcc aacctgcagc ccaggaatgg ttgcgtgaac cgagccaaaa aaaacctaca
     8101 agcgcagcag gaaggtaagc aaaaatgaat gcaaacgaat tgaacagcat gtggcaaaga
     8161 ggcaaggctt ggcccagcag gcaatatgcc ttttatctgg cgctcatctc gctatataac
     8221 gtcaggcaga aacatgttca gcaggtttgc gggaacatca acgtcgacta gggagagcac
     8281 gatcgaggct gaaacttcgc tttaccggag tgattattaa aggcagaaga agtttgcccg
     8341 ttttttccga tgaacgatag gtggatgaga ggcaagactt cccgtctcat tcacccttgg
     8401 tgaatcaagc agtggttgcg gcgccaacag gattatgaaa atgccagcgt tccagctgtc
     8461 gcccgggctt gaacgcggga tggggatagg aaaacagcta cgggcgggtt cgatcccgat
     8521 accgttctgc ttgctttagg gggtttgatt aatgaggctt tgattgccct gcagggttgc
     8581 ccacggctgc catagtagat tatccccata gccttaaccc ctcatcaacc agcctcggca
     8641 tgagtccaaa tgagatgtgg cgcatttctt taagagacgg ctttcaatgc cctcgttttc
     8701 ttagcttctg aatggcgctc aactgcgcga tggcctcgct caactcagct tgagcccgtg
     8761 catagtccat ggcggaagag cggtctttca tggcatcttc cgctcgcttc ttggcttcca
     8821 tggctttcat ctcgtcaaga tccgctccac gcattgcggt gtcggcgagg attgtgacga
     8881 cgtccggctg aatttccagc attccccccg atacatagac aagctcttct tccttcaacg
     8941 gtgccttgat tcggaccgaa ccgggcttga tgcgcgtaag catgggcgta tgctgcggat
     9001 aaacacccac ttcgcctcct tccgcaggtg caacaaggaa ttcagccggg cccgaataaa
     9061 ttgattcttc cgcgctgacg atatcaacat gaaacacgcc cattttagtt accattcact
     9121 ttgattagcc ggttcgctat ccgcgacaga aatatacgaa cagacatgtt attaaacagt
     9181 caattcctac tgcagggtct tggctttctc gaccgcttcg tcaatactcc caaccatata
     9241 gaaggcttgt tccggcagat tgtcatagtc cccggcgact atgcccttga atcccttaat
     9301 ggtttccttc aaaggcacat attttccagg cgacccggta aatacttcgg caacgttgaa
     9361 gggctgggaa agaaaccgct ggattttccg ggctctcgca accgcaagtt tatcctcggg
     9421 ggaaagctca tccatgccca gaatggcaat aatgtcacgc aactccttat aacgctgcaa
     9481 cgtctgctga accgctcgcg cggtattgta atgctcttcg cccaccacga gcggatcaag
     9541 ctgccgggat gtcgaatcca gcggatccac tgccggataa attcccagtg aagcgatatc
     9601 ccgcgataga acgacggtcg catccagatg gccaaaagtc gttgcaggtg aaggatcggt
     9661 caaatcatcc gctggcacgt agaccgcttg aatggacgta atcgaaccag acttggtgga
     9721 ggtgattcgc tcctggagac gtcccatttc ttcagccagg gtcggctgat atcccactgc
     9781 agacggcatc cggccaagca aggccgatac ttcggttccg gcaagtgtgt aacgataaat
     9841 gttatccacg aaaagcagca cgtcccggcc ttcgtcgcgg aaatattccg ccatggtcag
     9901 gcccgtcaat gccacgcgca aacgattgcc gggcggctca ttcatttgac cgtaaaccag
     9961 cgcgacctta tccaggacgt tggaatcctt catttcgtga taaaagtcgt tgccttcccg
    10021 ggttcgctca cctacacccg caaataccga ataaccgcta tgctcgatag caatattgcg
    10081 gatcaactcc atcatattga cggtcttgcc aacaccggcg ccaccaaaca atcccacctt
    10141 gccgcccttg gcaaacgggc acaccaggtc gataaccttg atgcctgttt ccagcagttc
    10201 cgtagacgcg gaaagctcat caaaatccgg cgctttccgg tgaatcgaca tcgttttttc
    10261 tgcatcgaca gggcccatct cgtcgatggg tcttccaagc acatccatga tcctacccag
    10321 agtcttggtg cccacgggca cacggatctg gtcgccagta ttctttacca tcatgccgcg
    10381 ccgcagtccg tctgacgagc caagcgcaat cgtgcgcacc acgccgtctc ccaactgttg
    10441 ctgcacctcg agtgtaagct cggacccctc gagcacgagc gcatcgaaga ccttcggcat
    10501 cccttcccga gtgaactcga cgtcaataac ggcgccgata cattggacaa tttttccctc
    10561 gttcatgtcg ttcctcaaat tctaagtaaa tggttaaacg gctgccgcgc ccccgacgat
    10621 ctcggatagc tccttggtaa tggcagcctg acgcgattta ttataaataa gcgtcagttc
    10681 atcgattaca ttgccggcat tatccgacgc tgctttcatc gccaccatcc gtgccgattg
    10741 ctcggatgcc atattttctg tcaccgcctg gtaaatgagc gcctcgacgt acctcaccat
    10801 gatatcgtcg atgacaggcc gggcttcagg ctcataaata taatcccaca ctcctcgctg
    10861 ccccttgggc ccttcaccca ccttgagccg ctcatcagaa agcgggagca actgttccat
    10921 gaccggctcc tgcttcatgg tatttacaaa gcgtgtgtaa aaaatgtata cgcgatcgaa
    10981 gcggtcctgc gtgtaaccgt ccagcaagac ttttactgct ccaatcagct tctccagatc
    11041 cggcgtgtca cccagacctg tcacgtgcga gatgacattc gcgccaatcc ggttcatgaa
    11101 tccgaaacct ttattcccga tacagcaaac ctcgatctgc tcaccctccg cttcccagga
    11161 tttcattttg tttacagcca tccggagaac gttggtattg agcccaccgc acaggccctt
    11221 gtctgaagtg accacgacga tcccgatccg cttgacggtg tcgcgctcga cgagaaaggg
    11281 atgtcgatac tcagtgtaag cccggctcat gtgcgctgcc acgttacgaa tcttgtcgcc
    11341 gtagggacgt gctttcctca tgcgctcctg tgcctttcgc atcttcgaag ccgccaccat
    11401 ctccatcgcg cgcgtgatct tctgcgtgtt ttttacgctc ttgatcttgt tccggatctc
    11461 tctgctgcca gccataaatt aatacgttcc attctttttg aaatcctgca ccgcggcatc
    11521 gaggtctttc tcggtggccg ggtcaagatc tttcgtggtt gcgatcttgt ccatgatcgc
    11581 cgaatatttg ctgcgcatga agcttcttag cgccgattcg aatgccaacg cccgttttac
    11641 ctcgacatca tcaagatacc ccttgtttac cgcaaatagc gtaagcgcca tttccggcac
    11701 gctcagagtg ccgtactggg gctgtttcat caactcggtg accatcttgc cccgttccag
    11761 ctgcttgcgc gtggcctcat ccagatcgga ggcaaattgc gcaaaagccg caagttcccg
    11821 gtattgcgcc aacgcgagcc gcacgccgcc accgagtttc ttgatgactt ttgtctgcgc
    11881 cgcaccaccc acgcgggaaa cagaaatccc ggcgttgata gcgggacgga tacctgcatt
    11941 gaacaagtcc gactcaagga aaatctgtcc gtcagtaatc gaaatgacgt tggtcgggac
    12001 gaaagcagtc acgtcccccg cctgggtttc gatcacagga agggcagtga gcgaacctgt
    12061 cttacccttg actgcaccgt tggtcgcctt ttcaacatat gccgcattga ctcgcgccgc
    12121 tcgctcaagc aagcgggaat gcaggtaaaa aacatctccg gggtaagctt cccgtcctgg
    12181 cggacggcgc agcaacaagg agatttgtcg gtatgcccac gcctgcttcg tcagatcatc
    12241 ataaacaatc aacgcgtccc ggcctgaatc ccggaagtac tcacccatcg tacagcccga
    12301 ataaggcgct atgaactgca ttgctgccga ttcggaagcc gtcgccgcga caacgatcgt
    12361 atattccatc gccccgtgct cttcgagctt acgcaccacg ttactgatgg aggaagcttt
    12421 ctgcccgatc gcgacataga tgcaagtcat gttctcaccc ttctgattga tgatcgcatc
    12481 aattgcaaca gcagtttttc cggtctggcg gtcaccgatg atcagctctc gctgaccacg
    12541 accgataggc accatcgaat ctatcgattt cagcccggtc tggaccggct ggttcaccga
    12601 ttggcgccaa accacgccag gagcgatttt ctcgataggc tccgagcgtt tgctaccgac
    12661 tgggcctttg ccgtcgatcg gttgcccgag tgcgttcacg acacggccta tcagttcctc
    12721 gcccaccggc acctcaagta tcctcccggt acacttgact gtgtcgcctt cggtaatatg
    12781 ctcgtattca cccagaacga ctgcccccac tgaatcgcgt tccagattaa gcgcaagacc
    12841 ataagtattt cccggaaact ccagcatctc cccctgcatg acatcggaaa gaccatggat
    12901 acgcacaatt ccgtcggtta ctgaaacaat cgtgccctga gtgcggattt cggacgtacc
    12961 ccccagcccc tcgattctgc tttttatcag ctcacttatt tcggatggat ttaaatgcat
    13021 tttttcaata actcctaggt tttcagcgcg atagccatgg cttccagctt tccccgcacg
    13081 gaagcatcca gcacttcgtc accaatttcg actttaacac cgcctatcaa ttcgcgatcg
    13141 acacgcacct cgggctcgat tttgcgcttg aatttatcct cgagatcagc caccaactgc
    13201 ttgagttgat cgtcgcttaa gggaaaggcg gaaataatct ttgcatccat cacgccctca
    13261 tactgggtcc gcaactgctc gaacaactcg cccacctcag gcaagatatc gatccggccg
    13321 ttctccgcca gcaataaaat aaagttgcgg ccctcgtcac tcaggcccgc tccgcaaatc
    13381 tccagcacca gttcgcctaa acgcttgggt gagatctttg gatcgccaat aagggcacga
    13441 atgttactgt ctgacgcgac tgttgcagct agttgcagca tatcagacca agccgccaat
    13501 tcgcgtttgg cttttgccag cttgaaaaca gcttcggcgt aaggcctcgc gacagtacgt
    13561 gcttcagcca ttgcctagag ctccgcttta atcgaagcaa ggagatcggc atgggccttc
    13621 gcgtcgactt cacggcggag aatcttggat gccccggcaa ccgccagctc tgccacgtgc
    13681 tgccgcaatg cttcccgagc gcgaaaaact tcctgctcga catctgcctt ggcgccaacg
    13741 agaatacggc tgccctcctc cttcgccgta ttttttgcct cgtccacaat ctccgcagca
    13801 cgtttttccg cctgagcaat aatatcggaa gctcgctgct tcgcttccct cacaacatcg
    13861 ccggagcgct gactcgcgag ttccagctca tgcttgcccc tctcccctgc tgccaggcca
    13921 tcggcaatgg ttttctgcct gttctcaatc gcgcgcatca acggaggcca cacaaacctg
    13981 accgtaaacc agataaatat ggcgaacgca aatgcctgag aaaaaagcgt gaagttgata
    14041 ttcatggcta acccctgacg cttagcacgc gcatgttatt tgatgacagc gagcaacgga
    14101 ttgccaaacg cgaagagcat cgccaacccg acgccaatga tgaacgaggc atcgatcaga
    14161 ccgagcagaa gaaacacttt gccttgcaat gtcgggatca tttccggctg acgcgcagcc
    14221 ccatccaaaa aactgctgca catgatacca atgccgatac aagcgccggc agcgccaaga
    14281 ccgatcatca ggccgatacc aatgccggtg taggcctgaa tcattgccag gaattgcaaa
    14341 ttgtccatct cgtttttcct ctcgtggtag tgaatagtaa ctggttagag cgcgaaccgg
    14401 agtcagtgcg actcgtgcgc catggacaga tacacgacgg tcagcatcat gaaaataaat
    14461 gcctgtagtg taacgatcaa aatatggaaa atagcccatc ccgctccgag aacggtgccg
    14521 aacagggtac cggacaaacc ggtagccgcc cacattccga gcagcagaaa aatgatttcc
    14581 cccgcgtaga tatttccata caaacgcaag gaatgagaaa ggggcttgga gacatattca
    14641 acaaggttga atagaatatt gaccggccaa aggatgggat tcttgccgaa aggggtgcaa
    14701 acgagctcat gtatccagcc acccaacccc ttgaccttca cattgaaaaa aatcatcagc
    14761 agccagaccg agagcgccag cgcaaaggtt gtattcacat cggcagtagg aacgctccgc
    14821 cagttgtgca agcctagttt tgcatatatc cacgccatga tgtcgatggg caggaaatcc
    14881 atcgcgttca tcatgaacac ccacacaaac acggtcagcg cggcaggggc tacaaaggcg
    14941 tgcctatcgc cgtaaaaagt acttttcacc tgctcgtcga tgaattcgac cgccaattca
    15001 acgaatgcct gccgcttgcc cggcacgccc gatgtcgcac cccgcacgac ccaccaaaga
    15061 aagccgaagc tgacgaggcc tgtcagcacg gccatcacca gtgtatcgac atgcagcgtc
    15121 cagaatccgc cctctcccac cgaagtggtc aaattcgtga ggtgatgggc catgtatgaa
    15181 gtaggagtga cttctgagcc tgatgccatc ttttacctga tcaatgatcc ttatctgttt
    15241 cgagcaatct tcgcgtcatc cgtcacaaat aatgccattc caaaaataat gacggtaagc
    15301 acgaacgttc cgataaatcc gactgcaact acatctttat aaaagctcat gacaagccac
    15361 aacagcgtga ccatgacaaa gacctttgct gcttcggcct tgattgccgt tatcagcacc
    15421 ccgcccgcgg tagaaccgcg ataccgggaa ataattgccg cgaaagcggc cgcggagatc
    15481 atgctgacaa ccccccccag caaggcggac gcggcgccat gcaaccccga aatcaggcca
    15541 agaacgagca ccattgccag agtggcgata aattgccatc gcatgacaat gcgtagaggt
    15601 ctattcccca ttcaatgcat tttggaatgt cagttagagg cagccgccca gcacaagatc
    15661 gagcttgaca ttctgttcct ggaagacctg attgcgcttc gaagacctga ttttatttcc
    15721 ctggctggcg atcctgacaa tcactctttt ggatcaccaa taagacgaaa ccgctaccca
    15781 tccgtttacg tacccagtac tggctacgtt taaacaacag aattttaatc tgtctgaggt
    15841 tttcagtcaa tcggaagcgg cttcaccaag aaataagtgc cgaaagcggg acgaggcgat
    15901 tgcgttaata gcgcggcgca tactgaagga aggcgtccac cttgccccgc acggggttgc
    15961 tatatatttc ttcccaattt tcttccccaa ctgcccgcaa acgcgctcgg cgctgctctt
    16021 acccatgggt atccggcgac ctgtaaaatt caacgaagaa acgaacatga cctaatggtt
    16081 ccacgctat
//
LOCUS       DASSHJ010000116         4110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1222053,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000116 DASSHJ010000000
VERSION     DASSHJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222053"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..807
                     /locus_tag="VFI43_04645"
     CDS             343..807
                     /locus_tag="VFI43_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HET7061448.1"
                     /translation="MKKVLLALALALSLASTIALADEETTAVGTPGDPAKSARTIEIT
                     MVDNRFNPSEVKVKEGETIKFLLKNSGRKKHEMMIGTAEEIDKHGRSMKKNPDAEHHD
                     EPNMITVEPGKTRELVWQFTKAGTVDFACPLPGHFKGMNFPGMKGTIKVEPK"
     gene            852..1289
                     /locus_tag="VFI43_04650"
     CDS             852..1289
                     /locus_tag="VFI43_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HET7061449.1"
                     /translation="MLSRGVIAAHASATVEVYKSSTCKCCAKWVDHMRANGFTVNTHD
                     VGNKEARERAGISTSLGSCHTALVDGYAVEGHVPADDIKRLLRERPRAIGLTAPGMPH
                     GSPGMEGARSDPYDVLLIDKQGDTKVYSRHLPSDPKASVMRLK"
     gene            1393..1980
                     /locus_tag="VFI43_04655"
     CDS             1393..1980
                     /locus_tag="VFI43_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7061450.1"
                     /translation="MNIRCFLLLALVTGFTAACGESKPPAASGSPAAPAAKPESPAGK
                     EVSPPAAESAKATVEPSSPAAAAAAPKNLAARKLDPEKIKRGEAVYLKNCASCHGPNG
                     EGTPGWRTPGADGRYPPPPLDGSAHAWHHSTDTLEEMIRKGSPGGAGGMPAWDGKLTN
                     QEIADVIVWIKSLWPDDVYEVWYKEIEHKPEPKQQ"
     gene            complement(2091..2474)
                     /gene="rplS"
                     /locus_tag="VFI43_04660"
     CDS             complement(2091..2474)
                     /gene="rplS"
                     /locus_tag="VFI43_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7061451.1"
                     /translation="MNLIEQLENEEISRLGKTIPDFSPGDTVVVNVKVVEGDRTRIQA
                     YEGVVIARRNRGLNSAFTVRKISSGEGVERTFQSYSPLISSIEIKRRGAVRRAKLYYL
                     RDRSGKAARIREKLPARASGKKEIQ"
     gene            complement(2522..3322)
                     /gene="trmD"
                     /locus_tag="VFI43_04665"
     CDS             complement(2522..3322)
                     /gene="trmD"
                     /locus_tag="VFI43_04665"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7061452.1"
                     /translation="MTHLIDVVTLFPEMFDAVTKFGITGRAKANGIYELHAWNPRDFT
                     EDNYRTIDDRPYGGGPGMLMLAQPLEKAIAAARDRQTARGTSRTRVVYLSPQGRLLNH
                     SVVMEFLEEPALVLLAGRYEGVDERLIARQVDDEVSIGDYVLSGGELAAMVLIDCIVR
                     QMPGVLGDSESANQESFVEGLLDYPHYTRPEVYSESKVPEILRSGNHASIRRWRLQQS
                     LGRTWLRRPDLLAKKVERGLTNEERALLEEFKKNCRIESNSGNSEEGN"
     gene            complement(3324..3836)
                     /gene="rimM"
                     /locus_tag="VFI43_04670"
     CDS             complement(3324..3836)
                     /gene="rimM"
                     /locus_tag="VFI43_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379932.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7061453.1"
                     /translation="MVVMGRVAGPYGILGWIRVVPYTEYVDTLADYPTWWLGRESGGW
                     EKVKVNECAVHGNALIASLEQHTDRNRAESLKGLDVAVPRSQLPVLSRNGEQGYYWSD
                     LIGLEVVNLQGESLGKVAGLLKTGANDVLQVQYLKESKRERLIPFISQVIVRVDQVAA
                     RITVDWGLDY"
     gene            complement(3841..>4110)
                     /gene="rpsP"
                     /locus_tag="VFI43_04675"
     CDS             complement(3841..>4110)
                     /gene="rpsP"
                     /locus_tag="VFI43_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7061454.1"
                     /translation="IRLARGGAKKRPFFNMVVADSRDRRDGKFIERVGFYNPRAAEGE
                     EGLRIQLDRVTFWQSHGAQLSDTVTKLVRRFNATHKDAGSSAQPS"
BASE COUNT          950 a         1171 c         1061 g          928 t
ORIGIN      
        1 catatatcat atctgcatta ccgcctcttg caattgaatc cttgccgatt gaaatccttg
       61 ccaattgaaa tccccgcata tgaaaacttc aaaaccatgt ttcccttttt gaaccaaaaa
      121 ggtgccctgc aagtctatcc gtggcgctga gtcgccctgc tgtatgtccc agtacgtgat
      181 tcgcaagagt aaaggggctt ttaccatgag aatgcaagcc gcagcatcag gctgggataa
      241 aaagaacctt tgtacgatat aaatcgggcc agccattggt cgaatgctgc ttttttttgt
      301 tatattgggc gagatgtcag cctcaaccat gaggaggatc ggatgaagaa ggttttgctc
      361 gcattggcct tggctttgtc tctagcctct accattgctc tggcagatga ggaaacgact
      421 gctgtaggga ctcccgggga tcctgccaaa tcggcgcgga cgatcgagat aacaatggtc
      481 gataaccgtt tcaatccttc ggaagtaaaa gtgaaggaag gtgaaacgat aaaattcctg
      541 ctcaagaatt ccggcaggaa aaaacatgag atgatgattg gtacggctga agaaatagat
      601 aagcacggca ggagcatgaa aaaaaacccg gacgcggagc atcacgatga gccgaacatg
      661 atcaccgtcg aaccgggcaa gacgcgggag ctggtatggc aattcaccaa ggctggaaca
      721 gtcgactttg cctgtccgct cccaggacac ttcaaaggta tgaattttcc tggaatgaaa
      781 ggaaccatta aagtagagcc caaatgaaaa tatctcctta ctctttacta gcacggtcaa
      841 ttctcgttgg tatgctctca cgcggtgtca tcgccgccca tgcttcagca acagtcgagg
      901 tctacaagag ctcgacctgc aagtgttgcg cgaagtgggt ggatcatatg cgtgcaaacg
      961 gcttcaccgt aaacactcat gatgtcggaa acaaggaagc gcgggaacgc gcgggaatat
     1021 cgaccagcct gggctcctgc cacactgccc tggtcgacgg ttatgccgtt gaagggcatg
     1081 tgcctgcgga tgatatcaag cgtttgctgc gggagcgtcc gcgagcgatc ggcctcaccg
     1141 cacccggcat gccgcacggt tctcccggaa tggaaggcgc gcgcagcgat ccttacgatg
     1201 tgcttctcat cgacaagcag ggagatacga aagtttatag ccgccatctt ccctctgatc
     1261 cgaaagcatc cgtcatgagg ctgaagtgag gactattcct atcaacacta tccatgcgca
     1321 tccattggaa aagcagacaa ggatcgtaat tgttttcttg agcatgaggc gctccgtgaa
     1381 gggctccagg ctgtgaacat ccgctgtttt ttactgctcg ctcttgtaac gggatttacc
     1441 gcagcctgcg gcgaatcgaa gcccccagcg gcgtctggct cgcccgctgc ccccgctgcc
     1501 aaaccggaat cacccgctgg caaggaagtc tctccgcctg cggctgaatc agccaaggcc
     1561 actgtcgagc ccagttctcc ggcagcagct gcggcagcac caaagaacct ggcagcacga
     1621 aagctggatc cagaaaagat caagcgcggc gaggccgttt acctcaaaaa ttgcgcgagt
     1681 tgccacgggc caaatggcga aggcactcct ggctggcgca ctcctggagc cgatggtcgc
     1741 tatccgccgc caccgctcga tggcagtgct catgcctggc atcattcaac cgatacgctt
     1801 gaggaaatga tcagaaaagg cagtccaggt ggagccggag gcatgcccgc ctgggacggc
     1861 aagctgacga atcaggaaat cgctgacgtg atcgtctgga tcaaatcgct gtggccggac
     1921 gatgtttatg aagtgtggta caaggaaata gagcacaagc cggaacccaa acagcaataa
     1981 ccggccgggc gaggctggca cgtggagttg tctcctctcc cccatgtgac cgtttccgcc
     2041 ctcctccgct ttccatcgaa tgcagaggag tgatgcggcc catctctgaa ttactgaatc
     2101 tcttttttac ccgaggcgcg tgccggaagc ttctcgcgaa tacgagcggc tttgccggag
     2161 cgatcacgca ggtaataaag tttggcgcgg cgcacagcac cccggcgctt gatctcgatg
     2221 ctagaaataa gcggagaata ggactggaag gttcgctcca cgccctctcc actggatatc
     2281 ttccgtaccg tgaaagccga gttgagcccg cgattccgcc gggcgatcac gactccttca
     2341 taagcctgga tccgggtacg gtcaccttca acaaccttga cgttgacgac gaccgtatct
     2401 cctggagaaa aatccgggat tgtcttgcca agccggctta tttcttcgtt ctcgagctgt
     2461 tcaatcaaat tcattgcgac tccttcccat gattcttacc tgtttatctg tatttcggtc
     2521 ttcagttgcc ttcttccgaa tttcccgaat ttgactcgat acggcagttt ttcttgaatt
     2581 cttccagcaa tgcgcgctct tcgtttgtga gcccgcgttc cacttttttt gccagcaagt
     2641 cgggccgccg cagccacgtc cggcccagcg attgctgcaa acgccagcgc ctgatgcttg
     2701 catgattgcc cgaccttaaa atttcgggga ccttgctttc ggaataaacc tcgggccggg
     2761 tgtaatgtgg ataatcgagc agtccttcaa caaaagactc ctggttggcg gattcggaat
     2821 cacccaaaac gcccggcatt tgccgtacga tacaatctat cagtaccatt gcagccagtt
     2881 cgccaccgga aagcacgtag tcgccgatgg aaacttcgtc gtctacttgc cgtgcaatga
     2941 gccgttcgtc cactccttca tatcgtccgg caagaagcac caaggctggc tcttcgagaa
     3001 actccataac cacactgtga ttcaggagcc ttccctgcgg tgaaagatag acgacacggg
     3061 tcctgctcgt gcccctggct gtctgcctgt ccctcgccgc agcgatggct ttctccaatg
     3121 gctgcgccag catcagcatc cccggccctc caccgtaagg acgatcgtcg atggtgcgat
     3181 aattgtcctc ggtaaagtcg cgcggattcc aggcgtgcag ctcgtaaatt ccgttcgcct
     3241 tcgcgcggcc ggttatccca aattttgtaa ctgcatcgaa catttcggga aacagcgtaa
     3301 caacgtcgat cagatgtgtc acgctaatag tccagacccc aatcgaccgt aattcgcgcg
     3361 gcaacctggt caaccctcac aataacctgg ctgatgaacg ggatgagtct ttcccgtttg
     3421 ctttctttca ggtactgcac ctgaagcaca tcgtttgcgc cggtcttcag caatccagcc
     3481 accttgccca gcgattcgcc ctgaagattg acgacttcga gcccgatgag atcggaccag
     3541 taataacctt gctcgccgtt ccgggaaagg acaggaagtt gactccgggg aaccgcaaca
     3601 tcaaggcctt tcaaggactc ggcccggttc ctgtcggtat gctgttcaag cgaggcgatc
     3661 aatgcatttc cgtgaacggc acactcattg actttaactt tctcccagcc gccgctttct
     3721 ctacccaacc accaggtcgg ataatccgcc agagtgtcta catactcggt ataaggaaca
     3781 accctgatcc agcccaatat accgtagggg cccgccacgc gccccattac caccatgggt
     3841 tcagcttggc tgggctgagc ttcctgcgtc tttatgtgtt gcattgaacc gcctgaccag
     3901 ctttgtcacg gtgtcggaga gctgcgcgcc atgcgactgc caaaatgtga cacggtcgag
     3961 ctggattcgc aggccttctt cgccttccgc cgcacggggg ttgtaaaagc ccacgcgttc
     4021 gatgaacttg ccatcgcggc gatcgcgtga atccgctaca accatgttaa aaaacgggcg
     4081 tttcttggcg ccgcctcgcg ccaatcgaat
//
LOCUS       DASSHJ010000117         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_344758, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000117 DASSHJ010000000
VERSION     DASSHJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344758"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(483..1721)
                     /gene="argJ"
                     /locus_tag="VFI43_04680"
     CDS             complement(483..1721)
                     /gene="argJ"
                     /locus_tag="VFI43_04680"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET7061455.1"
                     /translation="MPVNLEPPKPEQLLPIEGVSLGIAEAGIRKADRKDLLVIELSKS
                     ARVSGIFTQNRFCAAPVIVAKEYLSEQNPDHPIRALVINTGNANAGTGAEGVIHARKV
                     CAELARLFGCNDQQVLPFSTGVIMEPLPVEKIIAGLPAALVDLRANNWFAASQAIMTT
                     DTVPKGVSQQIQFNGITVTVTGIAKGSGMIRPNMATMLGYIATDAAVTQSLLDEILRY
                     AAANSFNRITVDGDTSTNDAFMLISTGSAGNEPLVDNTGEAFDLLRDAVTDVAAFLAQ
                     AIVRDGEGATKFITVKIEGGKTIDECTRTAYAIAQSPLVKTAFFASDPNLGRILAAIG
                     NAGLEELDVNKICLYLNDVLVAENGGRARGYREEDGQNVMKQSEIAIRVLLDRGESST
                     TVWTCDLSYDYVRINASYRS"
     gene            1888..2115
                     /locus_tag="VFI43_04685"
     CDS             1888..2115
                     /locus_tag="VFI43_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7061456.1"
                     /translation="MNCDKELDVRGLTCPLPILRTKKSLIDMTTGQVLKIIATDPGSV
                     IDFQVFADQTGNELLSLSETAGEFLFYMKKR"
     gene            complement(2201..2383)
                     /locus_tag="VFI43_04690"
     CDS             complement(2201..2383)
                     /locus_tag="VFI43_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET7061457.1"
                     /translation="MDARLLDILVCPLCKGPLLYKKSENELICKADRLAFPIKDGIPV
                     MLEDEARRFPPEIDIT"
     gene            complement(2498..2914)
                     /locus_tag="VFI43_04695"
     CDS             complement(2498..2914)
                     /locus_tag="VFI43_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7061458.1"
                     /translation="MNFQRGRQKEDPEINLVPMIDVLLVILIFLMITTTYSKFSELEI
                     NLPEAASEKPAEQTNAIDVGITATGSYLINGVTVKHTGSESLSKALREAAGNRSDPVI
                     VIHADAEATHQSVIRVMEAARQAGYNHVTFSTENPA"
     gene            complement(2930..3538)
                     /locus_tag="VFI43_04700"
     CDS             complement(2930..3538)
                     /locus_tag="VFI43_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7061459.1"
                     /translation="MFALIQAAGWPIWPIIFASIVALAIIVERLWALRLSAVTPRDLL
                     PKVLQEYRKNGVSEDMLAGLRKHSPLGQILAAGLKNDKSTREIMKESIEEAGRAVAHN
                     LERYLNTLGTIASLSPLMGLFGTLVGMIEIFGTNSPGSSNPAQLAHGISVALYNAAFG
                     ILVAIPSMIFYRYFHGKVDALIIEMELQALKLVEIVHGERQD"
     gene            3724..>4443
                     /gene="xseA"
                     /locus_tag="VFI43_04705"
     CDS             3724..>4443
                     /gene="xseA"
                     /locus_tag="VFI43_04705"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7061460.1"
                     /translation="MEMQIARGALSVSDLNRCAKELLESSFPLLWVSGEISNLRCYDS
                     GHWYFSLKDAGSQVRCVMFRDRNQYVDWEPRDGMQVDVRALVTLYQARGDFQLNVEAV
                     RRSGMGSLFEAFEQLKQKLGKEGLFDPDRKKKLPAFPRQIGIVTSPAGAALYDVLSTL
                     RRRMPSIPVVVYPTPVQGAGAGAKLAEAIQKAASRDECDVLILCRGGGSIEDLWAFNE
                     EIVARAIACCPMTIVSGVGHET"
BASE COUNT          984 a         1143 c         1207 g         1109 t
ORIGIN      
        1 gtcatggtca tcgtggcggc gggtatggct cgcgtgagcg gtccggatac agcggcgatc
       61 aatctagccg atccggctat gccagggaat ccatgtcagg ctataacgaa tatggcaccc
      121 gaaccggaga caacgagttg cagcacatga agccaatggg gcagtagcgc tagagcgcca
      181 tgagggggcc gcgaagcggt catgtagctg aaggggatgt cgccctgata aagggtgaga
      241 tgaagggaat ctcattatcg gttgcatctt tatgcacagg atcactgctt cgcccttcgc
      301 ggctgcatcc tggagcacag cgtgaggctc tcgtgagtgt aggtaccaga gtgtagtcgg
      361 aaaccagcga atccggcgga caacaaaggg atacgcagcc cggtctccta aatgcagcta
      421 cttttcgccc tccctccctc cttgagggag agagggtgaa ttccgacttc ctgctcctta
      481 atctaacttc ggtagcttgc gttgattctc acgtagtcat acgacaaatc gcaggtccag
      541 accgtagtgg aagattcgcc gcggtcgagc aaaacccgga tagcgatttc cgattgcttc
      601 atcacattct gcccgtcctc ttcccggtaa cctctggccc tgccgccatt ctccgcaacc
      661 aggacgtcat tcaggtacag gcatatcttg ttaacatcaa gctcctccag cccagcattg
      721 ccgattgctg cgagaatgcg gcccagattg ggatccgacg cgaaaaacgc agtttttacc
      781 aaaggtgact gagcaatggc ataagcagtt cgagtgcact catcgatcgt cttgcccccc
      841 tcgattttta ccgtaataaa cttcgttgcg ccttccccat cccgtacgat ggcttgcgcc
      901 aggaatgctg caacatctgt tacagcgtcc cgcagcaggt cgaatgcttc gccagtgttg
      961 tcaaccagag gttcattgcc cgcactaccg gtgcttatca acatgaaggc gtcattcgtg
     1021 gaggtatcgc catccacggt gatcctgtta aaagagtttg cagcggcgta ccggaggatt
     1081 tcatccagaa gagactgggt cactgcagca tccgtggcga tataacccag cattgtcgcc
     1141 atgttggggc ggatcatgcc cgaacctttc gcaattcccg tcaccgtcac ggtgatgccg
     1201 ttgaactgga tctgctgcga cacccccttt ggtaccgtat cggtggtcat gatagcctgt
     1261 gatgcagcaa accagttgtt ggctctaagg tctaccaagg cagcgggtag gccagctatt
     1321 attttttcta caggcaacgg ttccatgatc accccagtgg aaaacggcag aacctgctgg
     1381 tcattgcaac cgaacaggcg ggccagctcc gcgcacacct ttctggcgtg gatgacccct
     1441 tctgcgccgg tgccggcgtt tgcgttacct gtattgatga ctagtgcgcg gataggatga
     1501 tccggattct gttcagaaag atattccttc gcgactatca ctggcgctgc acaaaacctg
     1561 ttttgggtaa agatccccga aaccctcgcc gatttgctca gctcgatgac gagcaagtcc
     1621 ttgcggtcgg cttttctgat acctgcttcc gcgattccga gcgacacgcc ttcgatcggt
     1681 aataactgct ccggtttagg tggctccaag ttgactggca taaggaaata ttgatgttat
     1741 tgacctggac tattctcaat cggagtaacg gtgatattat ttcctccacc ttgcctccgt
     1801 tttgggagct catcctaact catccttggg ttttgcctcc acatttttat caaaaacaga
     1861 tttcagcagg agccaggaag taaattcatg aattgcgaca aagaactgga tgtgcggggt
     1921 ctgacttgtc ccttgcccat tttacgtacc aaaaaatccc tgattgatat gacgaccggg
     1981 caggtcctga aaatcatcgc cactgatccc ggttccgtga ttgattttca ggtgtttgcc
     2041 gatcagacgg gtaatgaatt gctgtcgttg tcggagacag ctggggagtt tctgttctat
     2101 atgaagaaga gataaagaga taagagagga aacaaattat cttatccagc ttgctttcga
     2161 tctcctgatc cctcaatact tgggcacttg ccggggggtt ctaggtaata tctatttccg
     2221 gtggaaatct ccgtgcctcg tcttccaaca ttacaggaat cccgtccttt atcggaaaag
     2281 ccagccgatc ggctttacaa atgagctcat tctcggattt tttatagagc aagggcccct
     2341 tgcatagcgg gcacaccaga atatcgagca atcttgcgtc catgacgtgc ataccttgag
     2401 ttgtggattc gttgaacact gccgggccaa ttctaagctg actcggcccc tggcctcgaa
     2461 atgcgatttt tcgttcagtc atcgctcggg ggagccctta tgccggattt tctgtactga
     2521 aagtaacgtg attgtaaccg gcctgtcggg cagcttccat cacgcgtatg accgactggt
     2581 gggtcgcctc ggcatctgcg tgaatgacaa tcaccggatc ggaacgattc cccgcggctt
     2641 cgcgtaacgc cttgctgagc gattcgctcc ctgtatgttt gaccgttact ccgttgatga
     2701 gatagcttcc cgtcgcggtt atgccaacat ctatagcatt cgtttgctcg gcaggctttt
     2761 ctgaggcagc ctccggcagg ttgatttcca gttcggaaaa tttggagtag gtggtcgtaa
     2821 tcattaggaa gattaggatg accagcaata cgtcgatcat cggaaccaga tttatttcag
     2881 gatcttcttt ttgtctcccg cgctggaaat tcatgctgtc ccggcaggat caatcctgcc
     2941 tttctccatg aacgatttcc acaagtttca atgcctggag ttccatttct atgatcagcg
     3001 cgtcaacttt tccatgaaaa taccggtaaa aaatcatgct tggtatcgct accaagatac
     3061 cgaaggcggc attgtagagt gcaactgaaa ttccatgggc tagctgggcg ggattgctgc
     3121 tgccgggcga gttcgtgccg aaaatttcta tcattccgac cagcgttcca aagagaccca
     3181 tgagcggact caaggaggcg atcgtcccga gcgtattgag gtagcgttca agattatgag
     3241 ccactgcacg gccagcttct tcgatggatt ccttcatgat ttcccgagtg ctcttgtcgt
     3301 tcttcaggcc ggcagcaagg atctggccca gtggggagtg ttttcgcagg ccggcgagca
     3361 tgtcctcgct caccccgttt ttccggtatt cctgcaggac cttcggtagc agatcgcggg
     3421 gagtgacggc gctcaggcgc agggcccaga ggcgctcgac gataatggcc agcgcaacga
     3481 tcgaagcgaa aataatcggc cagatcggcc agccggctgc ttgaattaat gcgaacacgc
     3541 catttcccga ggttcctcag caaccgctgt cataaataga gccgtaattt agcgtgcttg
     3601 cgcgatttca tcaataacag catcgctttt gccgaattgg ccgggtgtgc ctgcttgagg
     3661 acaggtcgtt ttgaaaggct gattgggtcc gttgtagagt agaatttgac aatgaatttg
     3721 atcatggaaa tgcagatcgc aaggggggcc ctgagcgtga gcgatctgaa tcgctgcgcg
     3781 aaagagcttc ttgaaagctc attcccatta ttgtgggtga gcggagagat atcgaacctt
     3841 cggtgttacg attccgggca ctggtatttt tctcttaaag atgctggctc gcaggttcgc
     3901 tgtgtcatgt ttcgtgacag gaaccagtac gttgattggg agcctcgcga cggcatgcag
     3961 gtggatgtgc gcgccctggt cactttatac caggcgcgcg gagacttcca gctcaatgtg
     4021 gaggctgtcc ggcgctcagg gatgggtagc ctgttcgaag ctttcgagca actcaagcaa
     4081 aaactgggta aggagggatt gttcgacccg gatcggaaaa agaaactacc agcttttcca
     4141 aggcagatcg gaattgttac ctcgcccgcc ggtgctgcgt tgtatgacgt tctgtcgaca
     4201 ttgaggcggc gtatgccctc cattcccgta gtcgtatatc caaccccggt acagggcgcg
     4261 ggggccggag caaaactggc agaggctatc cagaaggctg caagccgcga tgagtgcgac
     4321 gtcctgattt tatgcagggg tggcggaagc attgaagatt tgtgggcatt taacgaggag
     4381 atcgtggctc gcgcgattgc ctgctgcccg atgacgatcg tgagtggagt ggggcatgaa
     4441 acc
//
LOCUS       DASSHJ010000118        31669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_671068, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000118 DASSHJ010000000
VERSION     DASSHJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 31669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31669
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671068"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1122)
                     /gene="lptG"
                     /locus_tag="VFI43_04710"
     CDS             complement(37..1122)
                     /gene="lptG"
                     /locus_tag="VFI43_04710"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HET7061461.1"
                     /translation="MEAVPGAMTTIYRYLILQVLIGLAIATAILLPLFAFLDLLDQLD
                     DVGKGTFQVKDAFLYTALLLPRRFIQLAPFIALMGNVIALGRLAAGSELTVMRAAGIS
                     PIQISLAPLAVGLGLLFTDVLLDQFIAPQLQQQAVSNRAVALEKSAALGKGLGIWTRD
                     ERNILRIGEMLHGSRAVDIEIMHFDENGFLKSFTYAKYADFIGKDVWELSDVVTKTFV
                     GNRIESRSASSMKWESFLSSDDISTLTKAPESLSPFELFQHVRFLQSTGQEADAYALA
                     LWRKAGGALTALAMLLLSIPFVFGSVRAGLANRLILASMLGMSVYLFDQIVGNAGLLL
                     HVNPAIVALFPGLMLITLAGFWLRRIL"
     gene            complement(1098..2171)
                     /gene="lptF"
                     /locus_tag="VFI43_04715"
     CDS             complement(1098..2171)
                     /gene="lptF"
                     /locus_tag="VFI43_04715"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HET7061462.1"
                     /translation="MKIIERYITHELLLPFSVVVAILAILYGSFISASLLAGGVTESL
                     GILPMVKLILLRVLIALEVLMPISLYVAVVMGLGRLHRDQEIAVLRSAGVSERRIIYA
                     VLLVAIPTGIASGVISIFLRPWAYAESYILNAQAEAELNTDRFQAGRFYGSERKGRVI
                     YIGSKDDSGKQMNDVFHYIRKDGISEIIFAKEARQKEPAEGQRPQVDLSDGFVYRLAR
                     GGTKDTVVQFEKLAYFTDSGNAQYHRRKAAPTSALIGSDRPKDIAELQWRISRPLATI
                     LLALIAVPFSRSPPRKEKGEKKIFAAALVFAIYYILSGLAQTWVEQGTIGRLPGVWWL
                     YALMLILAVMSLAPLPWKRFLGR"
     gene            complement(2224..3036)
                     /locus_tag="VFI43_04720"
     CDS             complement(2224..3036)
                     /locus_tag="VFI43_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7061463.1"
                     /translation="MSQKGPFTAVVLAADRAAGDPIARMAGVACKAIAPVGGIPMIIR
                     VLDALEASGVVSKIIICGPPEHALSDCQPLLERIQSGRVTWVPNLNSPSRSANSALSG
                     IDRDAPVLLTTADHALLTPSIVRHFLSNAQAGGADAAVGVVKHEDVMAAFPGTKRTVI
                     RLRDGGFCGCNLYAFLNQRGRGLVSFWQRAEDLRKRPWLLIGQVFGPLLVISYLFGLL
                     TINRGLRAVSAKTGIRVLPVFLPFPQAGVDVDKVEDLRLVETVLNSDTRVQE"
     gene            complement(3050..4201)
                     /locus_tag="VFI43_04725"
     CDS             complement(3050..4201)
                     /locus_tag="VFI43_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7061464.1"
                     /translation="MNERPVPATAGIVVRPVKSLREMGVFIEVPWRIYADDPLWVPPL
                     RLERRLHFSRFNPFFKHGEWQAWVAYRDTQPVGRISAQIDHLHRERYGSDTGHFGLLE
                     GIESDEVFAALTKTAEEWLTTRGAKQISGPFNLSINQECGILVDGFDTPPVVMMPHSP
                     RWYGPLLEEQGYRPLKDLLAYWVNVDFEVPRFMSALVERFSGPIRLRVLNRARFSDEM
                     ELLRDIFNDAWSNNWGFVPFTREEFAELGNSLRMLVPDEAIAIAEVDGTPAAFMVGLP
                     NLNEIFSEMDGKLFPFGWRRLVSRLRSRSVHTARIPLMGVRKQFQSTPLGAALAFLVI
                     DALRETGRAWGIRAVELSWILEDNKPMRRMLEGIGCTEYKRYRIFGKSL"
     gene            4633..4887
                     /locus_tag="VFI43_04730"
     CDS             4633..4887
                     /locus_tag="VFI43_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061465.1"
                     /translation="MGAPEYNLLLALALIPVVIWLQVWIRNRRHSRRNETGHQEFDGL
                     GATLLSALIEGAAVVFSLILIIWIMGGVLRYFFPMIFNAK"
     gene            5069..6289
                     /gene="fabF"
                     /locus_tag="VFI43_04735"
     CDS             5069..6289
                     /gene="fabF"
                     /locus_tag="VFI43_04735"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7061466.1"
                     /translation="MRVVVTGIGIVSPIGTGTDEFWTSAVQGVSGIKKISSFDASHLR
                     SQVAGEVKGFDPASFLSPKYIEQTDRFTQLALLAAREALEDAESLDAYAPQRVAVSIG
                     SGMGGFGTFESSALRKFRNQPVPPFTVPRIMANSAAAWIAIKHGFKGVNLTCSTACSS
                     GANAIGMALDLLRSGRADAVIAGGAEACVLPLTINSFEILHALTTSSNSNPEKASQPF
                     AKGRAGFVMSEGAGILVLEREENARNRGAKTYARLAGFGQACDAAHIVMPDIKGQVAA
                     MRGAIEDAGLEPESVDYINAHATSTPLGDIVETRAIKDLFGKRASDIAISATKSMVGH
                     SIGASAAIGSVATIMSLHTGTVHPTINLDQADPECDLDYTPHEAQQKNPRVGLCNAFG
                     FGGNNASILFTTLQ"
     gene            6306..6560
                     /locus_tag="VFI43_04740"
     CDS             6306..6560
                     /locus_tag="VFI43_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7061467.1"
                     /translation="METADIEAKVIQFIATKVENLDASKINRSSKFEELGLDSMDTIQ
                     LLFDAEDAFGMSFDSEEAKGFSSVGDIVSYIEKHQASAAS"
     gene            complement(6615..9158)
                     /locus_tag="VFI43_04745"
     CDS             complement(6615..9158)
                     /locus_tag="VFI43_04745"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HET7061468.1"
                     /translation="MGKTLIIAEKPSVAADIARALGGFTKHTDYFESDQYVLSSAVGH
                     LLELAVPEAYEVKRGKWSFNNLPVIPPHFDLNPIEKSESRLKLLTKLIKRKDVEAIIN
                     ACDAGREGELIFQNIVRHVGTKKPIKRLWLQSMTPASIRDGFTKLLDSAAVQPLAEAA
                     VSRSEADWLVGINGTRAMTAFNSQEGGFYKTTVGRVQTPTLAILIEREEAIKNFTSRD
                     YWEVHATFAASAGKYTGRWFDERFSKDKKDSEQKAERLWDIEKAGFLRDKCENKPGTV
                     SDESKPATETCPLLYDLTALQRDANGRFGFSAKTTLALAQALYERHKALTYPRTDSRA
                     LPEDYAGTVTDILDSLDGSSYGVFAKQILKMGWVKPNKRIFNNAKISDHFAIIPTTLR
                     PKGLSEAEAKLYDLVTKRFLAIFFPSAEFMVTTRITRVEDEPFKTEGKILINPGWQAV
                     YGKEALTNGAGDTTLIKVAPDEKVLAEEVKIIASHTRPPARFTEATLLSAMESAGKLV
                     EDEELRTAMSAKGLGTPATRASIIEGLLSEHYLERVGRELQPTAKAFSLITLLKGLKV
                     PELTSPELTGDWEFKLRQIEQRKLQRDQFMKEIAGMTSHIVEQAKSYRGETISGNFST
                     LKSPCPKCGGIVQETYKKFQCQNCDFGLWKILAGRQFDTGEMEQLITQREIGPLQGFR
                     SKTGRLFNASIKLTDEFEMKFDFGGDAEDDAAQIDFSGLESLGQCPRCGNRVFDLGMS
                     YTCEKSTGAERSCNFKIGKIILKRPLEREEVIKLLQTGKTDLLTKFMSKKGRPFSAYL
                     VAGPDGKIGFEFEPKEPKPRRNATETAGEPQPPRAKPKIPSSKRTVRAK"
     gene            complement(9252..10331)
                     /gene="dprA"
                     /locus_tag="VFI43_04750"
     CDS             complement(9252..10331)
                     /gene="dprA"
                     /locus_tag="VFI43_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET7061469.1"
                     /translation="MSSIQDSESWIALCLVDGLGAESIRRLVDVFGTPGNIFEASVTS
                     LERVVKRRVAHNILQGPDQKKLLTARKWLECPGNSAVTLTDADYPSGLRNIADPPPLL
                     YLKGRRELLNAPVLAVVGSRSATPAGIVNAETFSEAMSNAGLCIVSGMASGIDTAAHR
                     GGLRGASSSIAVVGTGLDTVYPAANRSLAHELAEKGLLVSEFSLGTPPLGSNFPRRNR
                     IISGMSSGCLVVEAALQSGSLITARQALEQGREVFAIPGSIHSPLSRGCHSLIKEGAK
                     LVEKAEDILDELGFVISSQSLTGEGDEPNQESFLKHLGYDVMSIDLLSQRSGLTVETV
                     SAMLLSLELDGKVASLPGGVYQRVR"
     gene            10653..11156
                     /gene="def"
                     /locus_tag="VFI43_04755"
     CDS             10653..11156
                     /gene="def"
                     /locus_tag="VFI43_04755"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7061470.1"
                     /translation="MALLKILQYPDERLHTIASPVLEFNDEIRTLVQEMAETMYAAPG
                     IGLAATQVDVHKRIIVIDPSDTRDQLRVFINPEIVASSGQSDYEEGCLSVPGVFGKVA
                     RAERVTVEALDQYGERFMLEADGLLAVCIQHEMDHLLGKVFVEYWSWLKQTRIQTKLK
                     KQRQKVM"
     gene            11413..12351
                     /gene="fmt"
                     /locus_tag="VFI43_04760"
     CDS             11413..12351
                     /gene="fmt"
                     /locus_tag="VFI43_04760"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7061471.1"
                     /translation="MKVIFAGTPPFSAPALEALVNAGHEINLVLTKPDRPGGRGMRNI
                     PSAVKLLAEKYGFAVQQPESLKRTGVEAMLTEVAADIMVVVAYGLILPPSVLNLPKFG
                     CLNIHASLLPRWRGAAPIERAILAGDRETGISIMQMDQGLDTGAVLLQHPIPISENDT
                     TETVRERLSKLGAACILEVLTSIEQGATNAFPQNDEEATYAAKIDKSEAEIDWRSDAE
                     QIGRLVRAFNPYPGAYSRIRDISMKIWQASIKRDAKGEPGEILAIVPGGIVVASGDGG
                     VVLEIVQKSGAKRLPADQFLLGHPLQRGDRFECRHD"
     gene            12344..13621
                     /gene="rsmB"
                     /locus_tag="VFI43_04765"
     CDS             12344..13621
                     /gene="rsmB"
                     /locus_tag="VFI43_04765"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET7061472.1"
                     /translation="MIKAQSTAAESIAKVLKGESLTAVLQDVWRDQPGMSGQQRGAIQ
                     DLSYGVLRHYGQLDALLKLLLNKPVWNKDLHCLLLVALYQLIYSKSASYAIVDSAVSA
                     SNDFNGNKAVRSLTNAVLRNFLRNRPTLLAEVAGSEIGRFSHPQWWIDKLRAQYPDQY
                     QAILGAGNFRAPMTLRINRRRISSSEYQSLLREVGIEVRLSRDDAVELAQPISVDKLP
                     GFAEGLVSVQDMAAQMAGRLLDVHEGMRVLDACAAPGGKTAHLLELADVELTAIDDDA
                     IRAALVTQNLARLGLSAHCVMQGNASQSQKWWNDRQFDRILADVPCSASGVTRRHPDI
                     KWLRREDDIPRFASRQREILESLWQMLIKGGKLLYATCSIFAEENRLQVDQFLHRHSD
                     AHLLPLPEGETLEGQLLPNAHHDGFYYALLGKT"
     gene            13599..14222
                     /locus_tag="VFI43_04770"
     CDS             13599..14222
                     /locus_tag="VFI43_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HET7061473.1"
                     /translation="MRCWARPDLAERLLRPFAAIVVIALLAVMIPAAHAEGIIRVKSV
                     ALEAVDDGYQFDADFEISLNPKLEYALEKGMVLYFVTELNLVNSRWYWFDERMAQSRV
                     REGLSYYALTRQYRLSRGALYQNFATLKEALHALGRVRSRPIIASAELKPDVEYTVEL
                     RMRLDIAALPKPFQVETLSSKDWDLSTGILRWSTRLPAPRNHENARQ"
     gene            14219..16393
                     /locus_tag="VFI43_04775"
     CDS             14219..16393
                     /locus_tag="VFI43_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7061474.1"
                     /translation="MKYVIILAAGLGAILLFLLASAGADTDLFERKYRLLIRLNAGFV
                     LFLMGVVGYLLWRLRRRLSAGVFGSRLALRLLLVFSLMAILPGVLVYGVSVQFLSKSI
                     ESWFDVKVDRALEGGLDLGRTILDNRLEELRKKTKSAVLILGDQSEPPLKALTQIIDQ
                     SQVQQAAIFDQSGSVAAVVGADGSAEMVEPLSAEVIRRISEENIYSAVESVPGDGLYL
                     KVLMPFQAEGAPENTRILQLLQQVPEQLANEAELVQAAYRDYQELLLSREGLKSLYGV
                     TLTLALLLSLFSALAAAFLMSEKLSAPLGMLAEGTRAVAQGDFSRRHPVQSRDELGVL
                     TESFNLMTQQLEEARSTAQRNQQEVESARAYLENILANLSSGVLVFDASLRLRNANPS
                     AEQILRVPLSVLEGLTIEECTEREPELESFKNEILEGFHSGKKEEWQCQVERSRQGIN
                     QVLLLRGTRLPDVTGGGGVVIFDDITNLLQVQRTAAWGEVARRLAHEIKNPLTPIQLS
                     AERLQHKLSEKLAREDSQILKRSTETIVSQVEALKKMVNEFSEYARAPELEFHLLDFN
                     NLVKEVLALYESTHVTEAGSPQTRIFQALSPHLPSVRGDSVRLRQVIHNLLQNAQDTL
                     TDVAEPAILVRTEVSTDGVKFSVSDNGRGFPEQVKARAFEPYVTTKSKGTGLGLPIVK
                     KIIEEHNGSIQIENVRPSGAKISIILPIASSETGLEAAVSGR"
     gene            16538..17791
                     /locus_tag="VFI43_04780"
     CDS             16538..17791
                     /locus_tag="VFI43_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7061475.1"
                     /translation="MGNNTILVVDDEVGIRELLSEILRDEGYRVAVAENAGQARSWRK
                     QSRPDLVLLDIWMPDTDGITLLKEWASCGLLTMPVVMMSGHGTIDTAVEATRMGASGY
                     LEKPVPLQKLLSTVGKALRGGGEVKSKMSLPLSSLGRGPVIVELKKKLEQVANLRTPL
                     LLTSEPGTGVDVCARFLHHPNTPWVEPENLTALSDGPFSLLEQAKGGLLFLKEIGDLN
                     RLAQKGLFLLLGKLEKYNVRLVCATSMPLADLTAQGAYDVRLYEILSALCINVPALRE
                     HREDIPELASQILSRYIESGEVPVRQFSTAALNSLRNHEWPGNLAQLTGAVHSLALTS
                     PVDEISAEDVKQMLATSAPAQAIAGIPLNIPLREARDMFEKIYFEQLIEQENANMTRV
                     AERAGLERTHLYRKLKLLGIRLRNN"
     gene            18345..20381
                     /gene="tkt"
                     /locus_tag="VFI43_04785"
     CDS             18345..20381
                     /gene="tkt"
                     /locus_tag="VFI43_04785"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7061476.1"
                     /translation="MGTFRDFEAPVFKNLTSAIRALAMDAVEKAKSGHPGMPMGMAEI
                     AEVLWIHHLRHNPANPKWPDRDRFILSNGHGSMLIYALLHLTGYDLPMEEIKRFRQLH
                     SKTPGHPEYGYTPGVETTTGPLGQGLANAVGMALAEKLLASQFNRPGFDIVNHYTYVF
                     LGDGCLMEGISHEACSLAGTLGLGKLICFYDDNGISIDGHVEGWFTDDTPKRFEAYGW
                     HVVPNVNGHDPVAVEAAIEESKRITDRPSMICCKTVIGLGAPNKANTHEVHGAALGEA
                     EIAAARPHIGWSHLPFEIPDDIYSMWDAREKGKKLEDDWNRMFAEYAGQYPAEAAEFK
                     RRISGELPDGWHEHAEGLIARVNAKAETIASRKASQNAIEGLAPVLPELVGGSADLAG
                     SNLTLWSGSKPIDRSDGGNYIYYGVREFGMSAIMNGLSLHGGIIPYGGTFLMFSEYAR
                     NALRMAALMKIRNLFVFTHDSIGLGEDGPTHQPVEQTATLRYIPNMDVWRPCDTVEST
                     VAWARAIERLDGPSTLIFSRQNLPFQKRDAQTIELINKGGYILSEAADGAPQAVIIAT
                     GSEVSLAMMAQKALAESGVHVRVVSMPCTNLFDRQDQEYKDTVLPRGPGRVAVEAGIT
                     DYWRKYVGLEGAVIGMDTFGESAPANDLFSYFGFTVENVVKAVNSVIQSNHRVS"
     gene            20441..21442
                     /gene="gap"
                     /locus_tag="VFI43_04790"
     CDS             20441..21442
                     /gene="gap"
                     /locus_tag="VFI43_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7061477.1"
                     /translation="MTIKVGINGFGRIGRMVFRAAVQNFPDIEVVAINDLLEPDYLAY
                     MLEHDSVHGRFKGDVSVEDNTLVVNGRRIRLTAIKDPAELKWGEVGADVVVESTGLFL
                     TKESCEKHIAAGAKKVIMSAPSKDDTPMFVFGVNDSTYAGEPIISNASCTTNCLAPIA
                     KVLNDTWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPELNKKLTGMAFRVPTSDVSVVDLTVELEKDATYEEICAAMKSASEGSMKGVIGYT
                     DEKVVSTDFRGDSCTSIFDAEAGMALDDSFVKVVSWYDNEWGYSSKILEMVRVVAGK"
     gene            21555..22733
                     /locus_tag="VFI43_04795"
     CDS             21555..22733
                     /locus_tag="VFI43_04795"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7061478.1"
                     /translation="MSIIKVTDLDLKGKRVFIRADLNVPIKDGEVTSDARIAASMATI
                     NFCLEQGGRVMVTSHLGRPEEGVWSEENSLKPVAEDISARLGKRVRLIKNWVEGGFEV
                     DEGELVVLENCRFNKGEKKDLDETARKYASLCDVFVMDAFGTAHRAEASTYGIAKYAP
                     VACAGILLTEELEALTKALLNPARPMVAIVGGSKVSTKLTVLESLSDKVDQLVVGGGI
                     ANTFLKAVGKNVGKSLCEPDLVPTARNLMEKMAKRNASIPIAVDVVVGKKFDANEPAV
                     VKDSDDVADDDMIFDIGPKSAQTLADIIMKAGTVVWNGPVGVFEFDQFGNGTKTIATA
                     IANTDAFTLAGGGDTIAAIQKYGIYDKVSYISTAGGAFLEFLEGKKLPAVEILEQRGD
                     "
     gene            22771..24207
                     /gene="pyk"
                     /locus_tag="VFI43_04800"
     CDS             22771..24207
                     /gene="pyk"
                     /locus_tag="VFI43_04800"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7061479.1"
                     /translation="MIRRTKIVATLGPACRDPKVLERMINAGVDVVRINFSHGTREEH
                     IEFAELTRSLARSAGHAVGVLADLQGPKIRIGKFEEGKIVLKTGDKFVLDAECNLGNQ
                     ERVGLDYKELPGDVETGATLILDDGRIVLGVSDVRGQEIHCVVEQGGALSNNKGINRK
                     GGGLSAPALTSKDVEDIRTAAALKADYLAVSFPRSGEDMRWAREVMHEAGGKSLLMAK
                     VERSEAILALDDILQSSDAIMVARGDLAVEVGDAVVPALQKRMIRTARANNKLVVTAT
                     QMMESMINSPIPTRAEVSDVANAVLDGTDAVMLSAESATGQYPVESVAAMARVCLEAE
                     KEYLASNELRRLQGGLPETIEEAIARATMFTSGGLKIRAIAALTQSGRTVLLMSRRSS
                     NVPIFALSPQEDTRRKMTLFRGVYPIKFGGGSNDPEIILNHAENELLKRGAVRNGDLI
                     IMTIGEPVGKAGGTNTMKIVKVGEYRKA"
     gene            24280..25344
                     /gene="fba"
                     /locus_tag="VFI43_04805"
     CDS             24280..25344
                     /gene="fba"
                     /locus_tag="VFI43_04805"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646216.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET7061480.1"
                     /translation="MALVSLRQLLDHAAENGYGLPAFNVNNLEQIHAIMQAADECNSP
                     VIMQGSAGARKYAGEAFLRHLIEAAVESYPHIPIVMHQDHGASPSVCVNAIRSGFSSV
                     MMDGSLQADAKTPSSYEYNVDVTGKVVEMAHAVGVSVEGELGCLGSLESGMGEAEDGH
                     GAEGKLSHDQLLTDPEQAADFVKQTGVDALAIAIGTSHGAYKFTRKPTGDILAIDRVK
                     EIHERIPNTHLVMHGSSSVPQEWLDIIREYGGEMKETYGVPVEEIQEGIKYGVRKINI
                     DTDIRLAMTGAIRKHLAKNKSEFDPRKYLKDATAAAKEICKARFEAFGSAGQASKIKP
                     LNLESMANKYAKGELKAVIR"
     gene            complement(25532..27607)
                     /gene="ppk1"
                     /locus_tag="VFI43_04810"
     CDS             complement(25532..27607)
                     /gene="ppk1"
                     /locus_tag="VFI43_04810"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET7061481.1"
                     /translation="MSGAQTGNQFLNRELGQIEFNRRVLAQAENKDTPLLERLRFLCI
                     VSSNLDEFFEVRIAGLKEHIKLETPGFGPDGMLPNQVFKRVSEQTHSLVALQYQLLNQ
                     QILPKLAEEGIRFLRRSTWNEAQQEWIRAFFFRELMPVLTPIGLDIAHPFPRVLNKSL
                     NFAVELEGRDAFGRSSGTAIVQAPRVLPRLIPLPREISDSDYSFVFLSSILHAHVGEL
                     FSGMNVIGCYQFRATRNSELFVDEEEVKNLRIALQGELPQRHFGDAVRLEVADNCPKH
                     MSDFLLEQLGLSQEDFYQVEGPVNLVRLMQVPDWIDRPHLKYPPFIPGLPQTLQRKKA
                     VDIFQMIRKNDILLHHPYQSFQPVIHFIQQAAVDPDVVAIKQTVYRTGTDSLIMEALI
                     AAAGSGKEVTVVVELLARFDEEANINWASRLEEVGAHVVYGVVGHKTHAKMAMVVRRE
                     EGKLRRYIHVATGNYHASTARQYTDFGLFSCNEDFCGDVNEVFMQLTGLGKAGKLKHL
                     WQSPFSLHNQMLAAIHNEIENAKAGKPARIIAKMNALLEPEIIKALYVASAAGVRIDL
                     IIRGVCALRPGVKGLSENIRVRSVIGRFLEHSRIFYFRKGGAHAIYLSSADWMDRNFF
                     RRIEVCFPVLEEKLKKRVLSEGLTIYLKDNRQAWEMNGDGHYRLRTGRRGKRLCAQET
                     LLTELAAAK"
     gene            complement(27658..27939)
                     /locus_tag="VFI43_04815"
     CDS             complement(27658..27939)
                     /locus_tag="VFI43_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET7061482.1"
                     /translation="MARVVHCIKLGREMAGLDYAPYPGELGKRIFDNVSKEAWGGWIK
                     HQTMLVNELRLNLSDIKARKYLAEQLEAYFFGKGAEQPAGYVPPEKQEE"
     gene            complement(28050..29375)
                     /gene="argA"
                     /locus_tag="VFI43_04820"
     CDS             complement(28050..29375)
                     /gene="argA"
                     /locus_tag="VFI43_04820"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HET7061483.1"
                     /translation="MDFVAWFRSVAPYINAFRNKIFVIAFGGEVIADGKFVEFVHDLN
                     LLASVGVRLVLVHGARPQIESRLGERHLQTLYVKGMRVTDSEALQCVKESIGRIRVEI
                     ESLLSMGLPNSPMANAAIRVASGNFVTARPVGVIDGVDLMHTGEVRKIDAMAVRSRLE
                     QGELVLLSPLGYSPTGEIFNLTLEDVAAETAIALKAEKLIFLMDTQGIEKEDASSSGM
                     TLVRELTLAEGRAMLKEFRGQSTEPGDIQLYLPFAMRACEGGVPRIHLISRHVDGALL
                     QELFTHTGIGTMISEGPLQTLREAKIEDVGSILQLIEPLEADGTLVRRSRELLEIEID
                     HFVVIEHDKIIVGCAALYPFPDEQAAELACLTVHPDYRSSGCGEALLKNIEKRARAQG
                     NTNLFVLTTRTAHWFVERGFREVSVDELPKARQGLYNYSRRSKVFLKKL"
     gene            complement(29460..30224)
                     /locus_tag="VFI43_04825"
     CDS             complement(29460..30224)
                     /locus_tag="VFI43_04825"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7061484.1"
                     /translation="MAFPRIYYSDEISAESVIQLSGSATRHVVRVLRLQPGDQLILFN
                     GKGGEFRARIQRTQRDGATVAVEQYLGIERESPLVISLAQAVCAAEKMDWIVQKAVEL
                     GVSDIQVLATKLCTTKLSDERVDKRMAHWRQVAISACEQCGRNRIPQISPLATLPSWL
                     GSKMDEKSRLGSDHRNLRFMLSPYAEESLNCFTEGPSVSSVTFLVGPEGGLTREEEAA
                     AAVAEFAPLNLGPRTLRTETAGLTAIAVAQAVWGDF"
     gene            30458..31015
                     /locus_tag="VFI43_04830"
     CDS             30458..31015
                     /locus_tag="VFI43_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7061485.1"
                     /translation="MASLETPVCNFGWKAVDFDLPGVDGKRYNLASVKGENGLLVMFI
                     CNHCPYVKAVRDRIIRDVTDLQSYGIGAIAIMSNDPSDYPEDSFDNMIRVAREYHYPF
                     PYVWDGTQQIAKAYGAVCTPDFFGFNGDLELQYRGRLDASRKEAAPPHAHRDLFEGMV
                     QVATTGRGPENQIPSIGCSIKWRAE"
     gene            31019..>31669
                     /locus_tag="VFI43_04835"
     CDS             31019..>31669
                     /locus_tag="VFI43_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7061486.1"
                     /translation="MLNDLITIVKAVAQQEIMPRYMRVARQRKADGSLFTQADVIAQN
                     ALSRELTKLYPGPVVGEEMAEEEQKERWHAGSEGLWCLDPIDGTSNFVNGLPYFAVSV
                     AFMQKGKSVLGAVYNPVADEMFYAEQGKGAYLNGERLPISTRAATLRGAIANVDLKRL
                     GQKLAEAVCSARPFSSQRNYGSCSLEWCYTAAGRFDLYLHGGQKLWDYAAGCLILEE"
BASE COUNT         7432 a         8451 c         8581 g         7205 t
ORIGIN      
        1 aggcagggac gtatatgttt gaggaagaat cggtggctac aggatccgcc gaagccagaa
       61 gcctgcaaga gtgatcagca tcagcccggg aaaaagcgcg acaatagccg gattcacgtg
      121 cagcagcaat cccgcgttgc ctacgatctg atcaaacaga taaacactca tgcccagcat
      181 tgacgcaaga ataagcctgt tggcaagtcc cgcgcgaacc gagccaaaca cgaacgggat
      241 ggaaagcaac agcatcgcca gggcagtcag ggcaccaccc gccttccgcc acaatgcaag
      301 tgcatacgca tctgcttcct ggcccgtaga ctgcaaaaaa cgcacatgct gaaacagttc
      361 gaacggcgaa agactttcgg gcgccttggt gagggtggag atatcgtccg agctcagaaa
      421 ggactcccac ttcatcgagc ttgcgcttct ggactcaatc cgattcccga caaacgtctt
      481 ggtcaccacg tcgctgagct cccacacgtc cttgccaatg aaatcggcgt atttggcgta
      541 tgtgaaactt ttaagaaacc cgttttcgtc aaaatgcatt atctcgatat cgaccgccct
      601 gcttccatgc agcatctcgc ctatccgcaa aatgttccgt tcgtcacgcg tccagattcc
      661 taaacctttg cccagcgcag cgcttttttc aagcgcgaca gcgcggttcg aaacagcctg
      721 ttgctggagc tgcggtgcaa taaactgatc cagaagcaca tcagtgaata acaggccaag
      781 tcccactgca agtggagcca ggctgatctg aataggcgat atgcctgctg cgcgcatcac
      841 ggtaagctct gatcccgcag caagcctgcc caacgcaatc acgtttccca tcaaggcaat
      901 gaatggcgcg agctggatga atcttcttgg cagcaaaaga gctgtataaa gaaatgcatc
      961 cttgacctga aaagtccctt ttccaacgtc atcgagctga tcaagcaaat ccagaaatgc
     1021 aaacaacggc aacaatatcg cggtagcgat ggctagaccg atcagtacct ggagaatcag
     1081 atacctgtat atcgttgtca tcgccccagg aaccgcttcc atggaagtgg cgctaaagac
     1141 atcacggcaa ggatgagcat caaggcatat agccaccaca cgcccggcag tcggccgatt
     1201 gttccctgct ccacccaggt ttgcgcgagg ccgctcagga tatagtagat tgcgaaaacc
     1261 agagccgcgg cgaatatctt tttttcgcct ttttccttgc ggggagggga gcggctgaaa
     1321 ggcaccgcga taagagcgag caagattgtg gcaagaggac gcgagatccg ccattgcagc
     1381 tcggcaatgt ctttcggtct atcagaacct atcaatgcgg aggtaggagc ggccttgcgt
     1441 ctatgatatt gggcattgcc gctatccgtg aaataggcca atttttcaaa ctgcaccacc
     1501 gtgtcttttg tgccgccccg cgcgagtcgg tatacgaatc cgtccgataa atccacctgt
     1561 ggacgctgac cctcggctgg ctccttctgg cgagcttcct tggcgaatat gatttcgctg
     1621 atgccgtctt tcctgatgta gtgaaataca tcgttcatct gcttccctga atcatccttt
     1681 gacccgatat agatgaccct gcccttgcgt tcgctcccgt aaaatcttcc ggcctggaag
     1741 cgatcggtgt tcagctccgc ctctgcctgt gcattcagga tgtagctttc cgcatacgcc
     1801 cagggacgaa ggaaaattga aatgacgcca ctggcgatac cggtcggtat cgccaccagc
     1861 aataccgcat agatgatccg gcgttcactt acgccagcgg aacgaagcac ggcgatttcc
     1921 tgatccctgt gcaaacggcc aagccccatg acgacagcaa catataagga tattggcatc
     1981 aggacttcga gcgcgatcaa gacccgcagc aggatgagct tgaccatcgg cagaatgcca
     2041 agggattctg tcaccccccc tgccaaaagc gatgcgctga tgaaactccc gtacaagatc
     2101 gcgagaatgg cgactactac cgaaaacggc agcagcagtt catgcgtaat atatcgctcg
     2161 attatcttca caggcttgcg ctatgaatcc aggtttcccg cgcggctcag ctggtaccct
     2221 ggctcactct tgaacgcggg tatcactatt gagcacggtt tccacgagcc gcaagtcttc
     2281 aactttgtcg acatcgacgc cagcttgcgg gaaaggcaga aacacgggca agactcttat
     2341 accggtcttc gctgagactg ctcgaagccc gcgattgatc gtcagcaacc cgaacaggta
     2401 ggaaatgaca agcaaggggc cgaacacttg cccgatcagc aaccagggtc gcttgcgcaa
     2461 atcctctgcc cgttgccaga acgaaaccag accgcggccc cgttggttaa gaaacgcata
     2521 gagattgcat ccgcagaatc cgccgtcacg caaccggatc acagtccgct tcgtgccggg
     2581 aaatgctgcc attacgtctt cgtgtttgac aacgcccacg gccgcgtctg cgccgcccgc
     2641 ttgtgcattc gataaaaaat gccgcacgat ggatggcgtc agtaatgcgt gatcggcggt
     2701 cgtcagcaga acaggagcgt ccctgtcgat tcccgatagg gcgctgttcg cgctgcggct
     2761 tggggaattc agatttggca cccaggttac acgcccgctc tggatgcgtt cgagcagcgg
     2821 ctgacagtcc gaaagcgcgt gttccggcgg accgcatatt attattttgc ttactacgcc
     2881 gctggcttcc agggcgtcga gaacacggat aatcatcggg ataccaccaa cgggagcaat
     2941 tgctttacac gccaccccgg ccatccttgc gatagggtcg cccgcggcgc gatcggctgc
     3001 gagcactaca gccgtaaagg ggcccttttg gctcatgaga gataattgct caaagacttt
     3061 ttccaaagat gcgatagcgc ttgtactccg tgcagcctat gccttcaagc attcttcgca
     3121 tgggtttgtt gtcctccagg atccaggaca gctccaccgc ccgaataccc caggcccgtc
     3181 ctgtttccct caacgcatcg atgacgagaa aggcaagcgc cgcgccgagc ggcgtcgatt
     3241 gaaactgttt gcgcacgccc ataagaggta tgcgtgcagt atgaacgctt ctggatcgca
     3301 gccgactgac cagtcggcgc catccgaaag gaaacagctt cccgtccatc tcggaaaaaa
     3361 tttcgttgag attaggcagt cctaccataa acgctgccgg cgttccatcc acctctgcaa
     3421 tagcgattgc ttcatccggt acgagcatac gaagactgtt tcccagttcc gcgaattctt
     3481 cccgggtaaa gggaacaaaa ccccagttat tggaccaggc atcgttgaat atgtcgcgca
     3541 acagttccat ctcatcgcta aaccgggccc ggttcaacac tcgcaatcgt atcggccctg
     3601 aaaacctttc caccaatgca ctcatgaagc gcggcacttc aaaatccacg ttcacccagt
     3661 acgccagcaa atcctttaac gggcgatagc cctgctcttc gagcaaaggt ccgtaccatc
     3721 tcggggaatg aggcatcatg acgacaggag gcgtgtcaaa cccgtccacc aggatgccgc
     3781 actcctggtt gattgataaa ttgaacggtc cgcttatctg ttttgcgcct cgggttgtca
     3841 gccattcttc cgccgtcttc gtcagtgccg cgaagacttc gtcactttcg atgccctcta
     3901 gcagaccgaa gtgccctgta tcagatccgt aacgttcccg gtgcaaatgg tcaatttgcg
     3961 cactgattct tcctaccggt tgagtatccc gatacgcgac ccaggcttgc cattcaccat
     4021 gtttgaaaaa tgggttgaac ctggaaaaat gcaaacgcct ttccagccgc aatggcggca
     4081 cccacagagg atcatctgca taaatgcgcc atggcacttc aataaaaacc cccatttcac
     4141 gaaggctttt aacaggacgg acgacaatgc ccgctgtggc cggaacaggc ctttcgttca
     4201 tgatgagggg aatcgccgca atgcggttga tataaagagt tattggctac caggcctggc
     4261 tgctttttgc aaaaacacag cgggcagcgg cgtcagtaaa accattccac gaacggcatt
     4321 tccaatattc gtgccggttt caattctcag gaaggaatgt cgaacccgaa agtgacaact
     4381 cggtggtatg ctgatcacgt gtgatattat aattggtttt aggacagagt ggattttggg
     4441 ggtcgccgaa taaatcatgg gtgcagcgcg agagcgggga gcggtaatgc cattccccac
     4501 cgtttgattt gattggccct gattgacccc ttgcccttac ccacttgcca ctaccactta
     4561 cctctcgtaa aaacagttaa aaactctatg aaagattctg tctttgtcac gtcggaaaaa
     4621 ggttgattat caatgggagc tcccgaatat aacctgctgt tggcactcgc cctcatccct
     4681 gtcgtcatat ggctgcaagt atggataagg aaccgccgtc actcgcgtcg aaatgaaacc
     4741 ggtcaccagg aatttgatgg tcttggcgcc accctcttat ccgcgcttat cgaaggagca
     4801 gctgtggttt tcagcctgat cctcatcatc tggatcatgg gaggcgtttt aagatatttc
     4861 ttcccgatga tatttaatgc aaagtgatat caggaattcc gcattgcttt tctcgggcat
     4921 gccgtgttcc cgaccgcctt tacgccttcc cagctccggg gaaacttgtt ccagcgtgga
     4981 tgaccagcca ccagcgcgaa acgtaccccg gaacgtactg atctccttgc tgtttccaac
     5041 catctttgtt accaaatact ggccttctat gcgagttgta gtcacaggaa tcggaattgt
     5101 atcgccgatt ggaactggaa ccgatgaatt ctggacctcg gcggtacagg gtgtaagtgg
     5161 aatcaagaaa atctcaagtt ttgacgcgtc ccatttgcga tcgcaggtgg cgggagaagt
     5221 caagggattc gatcctgcct cctttctttc tcccaaatat atcgaacaaa ccgaccgctt
     5281 tacccaactg gcgttgctcg ctgcacgaga ggccctggaa gacgccgagt cccttgatgc
     5341 ttacgcaccg cagcgcgtag ctgtaagcat cggttcgggg atgggcggtt ttggcacatt
     5401 cgaatcttct gctctccgca aatttcgaaa tcagcccgtg cccccgttta cggttcctag
     5461 aataatggca aattcggcag cggcctggat tgcaatcaag cacggattta aaggagtaaa
     5521 cctgacgtgc agcactgcat gctcttccgg agcgaacgcg attggcatgg cgctcgatct
     5581 cctgcgttct ggacgggccg atgcagtcat agccggtggg gcagaggcct gcgtgcttcc
     5641 attgacgatt aatagctttg aaatattaca tgcgttgacg acaagctcga acagtaaccc
     5701 ggaaaaagcc tcgcagcctt tcgcgaaagg ccgggcgggt tttgtaatgt ccgagggtgc
     5761 aggcattctg gtgctggaaa gagaagagaa cgccagaaac cgcggtgcaa aaacgtatgc
     5821 gcggctggcg ggcttcgggc aagcgtgcga cgcggcgcac atcgtcatgc cggacatcaa
     5881 gggtcaggtc gcagcaatgc gtggggcaat cgaagacgcc ggtctggaac cggaatcggt
     5941 cgattacatc aacgcgcacg cgacctcgac gccgcttggc gatattgttg aaactcgggc
     6001 cattaaagac ctttttggca agcgagcgag cgatatcgcc atcagcgcga cgaagtcgat
     6061 ggtgggacat tcaatcggcg cctcggcggc tatcggcagc gtcgccacca tcatgtcgct
     6121 ccataccggg accgttcatc ctactattaa tctcgatcag gctgatcctg agtgtgatct
     6181 cgactacacc ccccacgagg cgcagcagaa aaacccccgg gttggcttgt gtaatgcttt
     6241 tgggttcggg ggcaataacg caagcattct tttcaccacc cttcaatgaa ggatgaagcc
     6301 agacgatgga aacagcagat attgaagcaa aggttattca gttcattgct acaaaggtag
     6361 aaaatcttga tgcgtccaag attaacaggt cttctaaatt tgaagagctg ggccttgatt
     6421 caatggacac cattcaactc ttgttcgacg ctgaagatgc tttcgggatg agcttcgata
     6481 gcgaggaagc aaaaggattc agctcagtag gagatatcgt ctcctatata gagaagcatc
     6541 aggcatccgc agcctcgtaa atgggcgaag gtcgggcaaa ggcggagcat tcaagcgctc
     6601 cggaaggtaa cccactactt tgcacgaacg gttctttttg acgagggaat cttcggtttc
     6661 gctctgggcg gctgcggctc acccgctgtt tctgttgcgt tccttcttgg ttttggttcc
     6721 tttggctcaa actcgaagcc tatcttgcca tcagggcctg cgacaaggta agcggaaaat
     6781 ggccgccctt tcttcgacat gaattttgtc aggaggtcgg tttttccggt ttgtaacagc
     6841 tttataacct cctcccgctc aagcggacgc ttaagtatta tcttgcctat tttaaaattg
     6901 caggaacgct ccgccccggt agatttttcg cacgtatagc tcataccaag gtcaaatacc
     6961 cggttcccgc agcggggaca ctggccaagc gattccagcc ccgagaaatc aatctgggcc
     7021 gcatcatcct cagcgtcccc gccgaagtca aatttcatct cgaactcgtc ggttaatttg
     7081 atgctcgcgt tgaagagccg gccggttttg ctgcgaaaac cctggagcgg cccgatttcc
     7141 cgctgagtga tcagctgttc catctccccc gtgtcgaatt gccgcccggc aagaatcttc
     7201 cataatccga aatcgcagtt ttggcactgg aatttcttgt atgtctcctg cactattccg
     7261 ccacatttcg gacatgggga tttcagggtc gaaaaattgc cgcttatcgt ttcgcctcga
     7321 tagcttttcg cctgctccac gatatggctc gtcatgccgg cgatttcttt catgaattga
     7381 tcgcgctgga gttttcgctg ctcgatctga cgcagcttga attcccagtc gccggtcagc
     7441 tctggcgatg tgagttcagg aaccttgagt cccttcaaaa gggtaataag tgagaaggcc
     7501 ttggcagtag gctgcaattc acgtcccacc cgctccaggt aatgttctga taggagtcct
     7561 tcgataattg aggcacgggt ggcgggcgta ccgagtccct ttgcactcat cgcggtacga
     7621 agctcttcgt cttcaaccag ttttcctgcg ctttccatcg ctgaaagcaa ggtcgcctct
     7681 gtgaatcgcg cgggggggcg ggtgtggcta gcaattattt ttacttcttc ggcaagcact
     7741 ttctcatctg gagcaacctt gatgagtgtc gtatctccgg ctccattggt tagcgcctct
     7801 ttcccgtaaa ccgcttgcca gccaggattg atgagaattt tgccttcagt tttgaaaggc
     7861 tcatcctcaa ccctggtgat gcgtgttgtg accatgaatt cggcggaggg aaagaaaatc
     7921 gccagaaagc gcttcgtgac gaggtcatac agcttggctt cggcctcgct caatcctttc
     7981 ggacgaagcg tggttgggat aatggcgaaa tgatccgata ttttcgcgtt attgaatatc
     8041 cgtttgttgg gcttgaccca ccccatctta agaatctgct ttgcaaacac gccataactg
     8101 gagccgtcga gagagtccag aatgtcggtg accgtgccgg cgtagtcttc gggcaatgcc
     8161 cgcgagtccg tacgaggata agtcaatgcc ttgtgccttt cgtacaaggc ttgagcaagt
     8221 gcaagcgtcg ttttggccga aaaaccaaag cgcccattcg catcgcgctg taaagcagta
     8281 agatcgtaaa gcaaaggaca cgtttctgtc gctggtttgc tttcatcgct caccgttccc
     8341 ggtttgttct cgcacttgtc ccggagaaac cctgcttttt caatgtccca taagcgctcg
     8401 gctttctgct cagaatcctt tttgtctttc gaaaaacgct catcaaacca gcgtccggtg
     8461 tattttccgg cgctggcggc aaatgttgca tgcacttccc aatagtcgcg cgaggtaaaa
     8521 ttcttgatcg cttcttcccg ctcgataaga atcgccaagg tcggagtctg cacccggccc
     8581 acggtggttt tatagaatcc gccctcctgc gaattgaagg ccgtcatggc gcgcgtaccg
     8641 ttaataccga cgagccagtc cgcctcggat cggctcacag ccgcctctgc aagcggctga
     8701 acagctgcag aatcaagcaa ttttgtgaat ccgtctcgga tcgaagcagg cgtcatcgat
     8761 tgcagccaga gtctttttat cggcttttta gtaccgacgt ggcgcacgat gttctgaaag
     8821 atcaactccc cttcccggcc cgcgtcacaa gcattgatga tggcctccac gtcctttcgc
     8881 ttgatgagct tcgttaagag ctttagcctt gattcgcttt tttctatcgg attcaaatcg
     8941 aaatgagggg ggatcacggg caggttattg aagctccatt ttcccctttt tacctcgtat
     9001 gcctcaggaa ctgccagctc aagcaggtgc ccgacggccg aagatagaac atactgatca
     9061 ctctcaaagt aatccgtatg cttggtaaaa cctccgagcg cgcgcgcgat gtcagcggct
     9121 acagacggtt tctcagcgat gatcaaggtt ttacccataa acttccatag atattacggc
     9181 ttgcggaaca aggtcgagca ggtaaagcac gcgtactgcc agtattcttc gaaacccaag
     9241 ctctggaaca gctatctaac acgctgataa acgccgcccg gaaggctcgc caccttccca
     9301 tccaactcca acgacaatag catcgctgat acggtttcga ccgtcaagcc gctgcgttga
     9361 gaaagcaaat caatactcat gacatcgtag cccaagtgtt tcagaaatga ttcttggttc
     9421 ggttcgtcgc cctcaccggt tagactctga ctgctgatca cgaatccgag ttcatccagg
     9481 atgtcctccg ctttttcgac aagctttgcg ccctccttga taagggaatg gcatcccctg
     9541 gacaacggag agtgtatgga tccggggatg gcaaatactt ccctcccctg ctccagcgcc
     9601 tgccgggcgg taatcaggga gccgctttgc agggcagcct ccaccaccag gcaaccactg
     9661 ctcatgccgc tgatgatccg gtttcgccgc ggaaaattgc ttcccagagg cggggttcca
     9721 agagaaaact ccgagacgag aagccctttt tccgcgagct catgggcaag gctccggttg
     9781 gcggccggat aaacggtgtc gagcccagtg ccgaccaccg cgatgctgga tgaggcgccc
     9841 cgcaagcctc cacggtgagc agctgtgtct attccgcttg ccattccgct aacaatgcaa
     9901 agacccgcat tgctcattgc ttccgagaat gtttcagcgt tgacgatacc tgcaggtgtg
     9961 gcactgcggc tgcctacaac agcaagcaca ggcgcattga gcaattcgcg tctgcctttg
    10021 agatagagaa gcgggggtgg gtccgcgata ttgcgcaatc ccgagggata atctgcatcc
    10081 gttagcgtaa ctgcggaatt tcccgggcac tccagccact tgcgtgcggt caaaagcttt
    10141 ttttgatccg gaccctgcaa aatgttgtgc gcaacccttc gtttcaccac acgctcgagt
    10201 gaagtgacac tcgcttcgaa aatattgcct ggtgttccga aaacatcgac gagccggcgg
    10261 atggactccg ccccgaggcc atcaaccaaa caaagagcga tccagctttc gctatcctgt
    10321 atcgagctca tcatgcttat cacaacgaaa tttgcttcga tcccgagtct ggcgcgctga
    10381 caatggatgt gaaccccatt ccggcgcggt cgaggtgagg gcgtggtagt gtatcatcca
    10441 taaatcagct tcttgcaacg cttccaggtt tgagtttcgg cgcgagtacg cggactaaaa
    10501 gcaggttgcc tatcaaatca cgatataata actcgctgtt cgcctgcatc atttggatca
    10561 acaaaatgcg caacagggga tttgcgcacc agccgggaat aagctttgag cggtgcattt
    10621 caagtttcct ttgaactaac caagcctctc tcatggcgct cttgaagata ttgcagtatc
    10681 cggacgaacg gctgcatacc atagcatccc ccgtactgga atttaacgac gagattcgca
    10741 ccctggttca ggaaatggcg gagacaatgt atgctgctcc tggaattggg ctggcagcca
    10801 ctcaggtgga tgtgcataag cgcatcattg taatcgaccc atctgatacc cgcgatcaac
    10861 tgcgcgtgtt catcaatccc gaaatcgttg cgagcagcgg gcaatcggat tacgaggaag
    10921 gatgcctgtc agtccccggg gtgttcggca aggtagcccg cgctgagagg gtgactgttg
    10981 aagcgcttga tcaatatgga gagcgtttca tgctggaggc cgacggtctg ctcgccgttt
    11041 gcatacagca tgaaatggac catctgctgg gaaaggtgtt tgtcgagtat tggtcatggc
    11101 tgaagcaaac ccggattcag acaaagctga aaaagcagcg gcaaaaggtg atgtgatcta
    11161 ttcgcagcgc tgcccccctg acccgtacgc atgttatatg ttcttcgaac gattgtgaac
    11221 gccatattcg tttgattggt cggcaggaaa atcaatataa acgcaatgcg gccgggtgcg
    11281 aagtgctcgt cgcgcttttc ggggtacgga atgcaggagc gcgtttccga aacacccatt
    11341 ttccgacttc atggatgaac gtgtttcccg tcagtccgtc gcaggtattc aagcccgtac
    11401 tgagcaaaat tcatgaaagt cattttcgca ggcactccgc cgttttccgc tcctgcgctt
    11461 gaggccctcg taaatgccgg gcatgagatc aatcttgttc tcacaaagcc tgaccgtccg
    11521 ggtgggcggg gaatgagaaa tattcccagc gcggtaaaat tgctggcgga aaaatacggg
    11581 tttgccgtgc aacagcccga atcgctcaaa cgcacggggg tggaggccat gttgaccgag
    11641 gtcgccgccg atatcatggt agtggtagcg tacggactaa tcctgccccc gtctgttcta
    11701 aatcttccga agtttggttg ccttaatatt catgcgtcct tgctgcctcg ctggcgaggt
    11761 gctgctccga tcgagcgggc aatcctggcg ggggaccggg agaccggtat ctccatcatg
    11821 caaatggacc agggtcttga tacaggggcg gtcctgttgc aacatccgat tccaatctct
    11881 gaaaacgata caaccgaaac cgtacgtgaa aggctctcca agctcggcgc tgcctgcatc
    11941 cttgaggtac tgacttccat cgagcagggc gcgaccaatg ctttccccca gaacgacgag
    12001 gaggcgactt acgcagccaa gatagacaaa agcgaagccg aaattgactg gcgttcggac
    12061 gcagagcaga tcggccggct ggtaagagct ttcaatccgt atccgggagc atactcgagg
    12121 attcgggata tctcgatgaa aatatggcaa gccagcatca agagggacgc aaaaggtgag
    12181 ccgggagaaa ttctcgcgat agtacctggc ggaatcgtcg tcgcttcggg cgacggggga
    12241 gtcgttctgg agatcgtcca gaaatccgga gccaagcggt tgcccgctga ccaattttta
    12301 ttgggccatc ccctgcagcg cggcgaccgc ttcgaatgcc ggcatgatta aagcccagag
    12361 taccgctgct gagtctatag cgaaagtgct caagggggaa agtttgactg ccgtattgca
    12421 agatgtctgg cgagatcaac cggggatgag cggtcagcaa agaggggcga tacaggattt
    12481 gagctatggg gtgctgcgcc attatggaca actcgatgcc ttgctcaaat tgttgctgaa
    12541 caaaccagtg tggaacaagg acctgcactg tttattgctt gtcgccctat atcagctcat
    12601 ctatagtaaa agcgccagtt acgccatcgt agatagtgca gtctcagcct ccaatgattt
    12661 caacggtaat aaagcggtaa gaagtctgac caacgcggta ttgcgtaatt tcctacgcaa
    12721 ccgcccaact ctgcttgccg aagtggcggg aagcgagatt ggacgttttt ctcatccgca
    12781 gtggtggatc gataagctgc gcgcccaata tcctgatcaa tatcaggcga tactcggggc
    12841 gggtaatttt cgcgcaccga tgacactcag aatcaaccgg cgcaggatta gctcttctga
    12901 gtatcagagc ctgttacgcg aggtgggcat cgaagtaagg ttgtcccgtg acgatgcagt
    12961 cgaattggcg cagcctatct ctgtggataa actgccggga tttgccgagg gattggtttc
    13021 cgtacaggat atggctgccc agatggcggg acggcttctg gacgtgcatg agggaatgcg
    13081 cgtcctggat gcttgtgccg ccccgggagg caagaccgcg catctgctgg aattggctga
    13141 tgtagagctg acggcgatcg atgacgacgc tatccgcgcg gcccttgtta cgcagaatct
    13201 cgcgcgcctc gggctctcgg ctcattgcgt tatgcagggc aatgcttcgc aatcccaaaa
    13261 gtggtggaac gaccggcaat tcgatcgaat tcttgctgat gtgccctgct ctgcctccgg
    13321 agttactcga cggcatccgg atataaaatg gctcaggcgg gaagatgaca ttccccggtt
    13381 tgcctcaaga cagcgggaga ttctcgagtc gctatggcag atgctgatca agggcgggaa
    13441 gttgctttat gctacctgct cgatattcgc cgaagaaaac cgcttgcagg tggatcaatt
    13501 cttgcatcgg cattccgacg cccatctttt accccttccg gagggggaga cactcgaagg
    13561 ccaactattg cctaacgctc accacgacgg tttttattat gcgctgctgg gcaagacctg
    13621 atctcgccga acgattattg cgcccttttg cagcaatagt agtaattgca ttgctggcag
    13681 tgatgatccc ggcagctcat gcagaaggaa ttatccgggt taaatcggtt gccctggaag
    13741 cggtagacga cggttatcaa ttcgatgctg atttcgaaat ctcgctcaac cccaagctgg
    13801 aatatgccct ggaaaaaggg atggtcctgt actttgtgac cgagcttaat cttgtgaatt
    13861 cgcggtggta ttggttcgat gaaagaatgg cccagagcag ggtccgggaa ggactgagtt
    13921 attatgcttt gacgcgccaa taccgtttga gccggggcgc gttgtaccag aacttcgcca
    13981 cactcaaaga agcgttgcat gcgttgggac gggttcgcag ccgtcctatt attgcaagtg
    14041 ccgaactgaa gcccgatgtg gaatatacgg tggaattgcg catgcggctt gatatcgctg
    14101 ccttgcccaa gccattccag gtggaaacac tcagctcgaa ggattgggat ctgagcaccg
    14161 ggattctgcg ttggagcacg cgattgccgg cccccaggaa ccacgagaac gcgcgacagt
    14221 gaagtacgtc attattctcg ccgccggcct cggcgcaatt ctgctgtttt tgcttgcaag
    14281 cgcaggtgcc gataccgatc tgttcgagcg caagtatcgc ctgctgattc ggcttaatgc
    14341 cgggtttgtg ttattcctga tgggcgtagt gggctatctc ctgtggcggc tcagacgcag
    14401 gctgagcgct ggagtcttcg gatccagact cgccttgcgt ttgctactgg tcttttcctt
    14461 gatggccatc cttccaggcg tgctcgtata cggtgtgtcg gtgcaattcc ttagcaaaag
    14521 catcgagtcg tggttcgatg taaaggttga tcgtgcgctc gagggcggat tggacctggg
    14581 gcgcacgatc ctggacaatc gactggagga actgaggaag aaaaccaaat ctgcggtgct
    14641 cattctcggg gatcaatcgg agccgcctct gaaggcgctc acccagatca tcgatcagtc
    14701 acaggtgcag caggcagcca tattcgatca gagcgggtct gtggctgccg ttgtgggtgc
    14761 ggatgggtcc gcagagatgg tggaacccct cagtgcagaa gtgatcaggc gcatcagtga
    14821 ggaaaacatc tacagcgccg tggaatcggt gccgggcgac ggcctctacc tgaaggttct
    14881 gatgccgttc caggctgaag gcgcgcccga aaatacgcgg atattgcagt tattacagca
    14941 ggtacccgag cagctagcca atgaagcgga attggtgcaa gcggcctatc gcgattacca
    15001 ggaattgctg ttgtcacgag agggcctgaa gagtctatac ggcgtaacgc ttaccctcgc
    15061 gctgctgctc tcgctgtttt cggcactggc cgcagcgttc ttgatgagcg agaagttaag
    15121 cgctccgctg ggaatgctag ctgaagggac gcgtgcggtg gcgcagggag atttcagcag
    15181 acggcacccg gtgcaaagcc gggatgagct gggagtgctc accgagtcgt tcaatctgat
    15241 gacccagcaa ctggaagaag cccggagcac cgcgcagcgg aatcaacagg aagtcgaaag
    15301 cgccagggct tacctggaaa atatcctggc caacctgtca tccggagttc tggttttcga
    15361 cgcaagcctg cgcctgcgaa acgcgaatcc cagtgccgag caaatcctcc gcgtgccatt
    15421 atcagttctt gaaggtctca cgattgaaga atgcactgaa cgggagcctg aactcgagtc
    15481 tttcaagaat gagatcctgg aaggctttca ttccggaaag aaagaggagt ggcaatgcca
    15541 ggtagagcgt tcgcgacagg ggatcaacca ggtattgctg ttgcgtggaa cccgtcttcc
    15601 agacgtcaca ggtggcggag gtgtggttat ttttgacgac attaccaatc tgttgcaggt
    15661 gcagcggaca gctgcctggg gagaggtcgc acgacgtctg gcccatgaaa tcaagaatcc
    15721 actgacgccg atacagctat ctgccgagcg tctgcaacac aagctttccg agaagcttgc
    15781 cagggaagat tcacaaattc tgaagcgctc aactgaaacg atcgtgagtc aggtcgaagc
    15841 gctgaaaaaa atggtcaatg aattcagcga atatgcgcgg gcgccggaac tggaattcca
    15901 tttgctggac ttcaataatc ttgtgaaaga agtgctcgct ctgtacgaat cgacgcatgt
    15961 aacggaggcg ggatcgccac agacccgtat atttcaggcc ctcagtccgc acctgccgtc
    16021 agtcaggggc gattctgtcc gtttgcgcca ggttattcat aatctccttc aaaatgcaca
    16081 ggacacactc acggatgttg cagaacctgc cattctggtc cgcaccgaag tctcgacgga
    16141 tggagtgaaa tttagcgtaa gcgacaatgg cagaggattt cctgagcaag tcaaagctcg
    16201 ggccttcgag ccctatgtaa cgacaaaatc gaaaggaaca ggattgggac tccccatcgt
    16261 caaaaaaata atcgaggagc acaatggatc gattcagata gaaaatgtcc gcccctccgg
    16321 agcaaaaatc tccatcatac tgcccattgc gtcgtccgaa actggactgg aggcagctgt
    16381 aagcggacga tgattacatt tccccacgtc tatctttatc attacgctcc cgtccccctg
    16441 cagaagcttt attgccgggc cttcgctaca tgcaggcgag cggcagcagg aacaattcaa
    16501 ggtatccctg atcctttaaa ctgactctta aacccccatg ggcaataaca cgatacttgt
    16561 cgtcgatgat gaagtaggca tacgcgaact gctatcggaa atactgcgtg atgaaggtta
    16621 tcgcgtagcg gtcgccgaaa atgccgggca ggcacgcagt tggcgaaagc agtcacggcc
    16681 tgacctggta ctgctggata tctggatgcc ggatacggat ggaattacgc ttttaaagga
    16741 atgggcaagc tgtggactcc tgacaatgcc ggttgtgatg atgtcaggcc atggcaccat
    16801 agacacagcg gtagaagcga cgcgaatggg cgcatcgggt tacctcgaaa aacccgtacc
    16861 gctgcaaaaa ctgttgagta cggtaggaaa agcgttacga ggcggcggcg aagtcaagtc
    16921 gaaaatgtct cttcctcttt caagcctcgg aagaggcccc gtcattgtcg agttaaaaaa
    16981 aaagctggag caggttgcca atctcaggac tcccctattg ctcacaagcg aacccggaac
    17041 cggcgttgat gtatgtgcac gatttttgca ccaccccaat acaccctggg tagaaccgga
    17101 aaatcttacg gcactatcag acggcccgtt ttccctgctt gagcaggcca aaggcgggct
    17161 gctgtttcta aaggaaatcg gcgatctcaa caggctggcg caaaaaggat tatttctcct
    17221 gctggggaag cttgaaaaat ataacgttcg cctggtctgc gcgacttcca tgcctcttgc
    17281 ggatcttacg gctcagggcg cgtatgatgt aaggctatat gaaatattga gcgccctatg
    17341 tattaatgtt cccgccttga gagagcaccg tgaggatatc cccgagcttg caagccagat
    17401 cctgtcgcgc tatatcgagt cgggggaagt cccggtacgg caattcagca ctgcggcgtt
    17461 aaattcgctg cgaaaccacg agtggccggg taatcttgcg cagctcacgg gcgcggtaca
    17521 ctccctcgca ctgacctctc ccgtagacga aatatcggcc gaggatgtaa agcagatgct
    17581 ggcgacctcg gcgcccgcgc aggcgatagc cggtataccc cttaatattc cactgcgcga
    17641 agcgcgcgac atgttcgaaa aaatttattt cgagcaactc attgagcagg agaatgccaa
    17701 tatgactcgg gtggcggagc gtgcaggtct cgagcgcact catctttacc gcaagctcaa
    17761 gctgctgggt atcaggctgc gtaataatta ggaacctctg aataagtcat catgggcgcg
    17821 acgacggctt tgcgggatgg gatgcgcgaa aggcgacgaa gcgaatggac aatcccttcg
    17881 cgaggagcct gacaaagcag actgcctgca aagcccagtc ccatacgggt tgcggcaaaa
    17941 tcgcgcgctc actgcgttgc actccttggc atcgtaggcc ccagctacga cctgcgtcgt
    18001 gcgccttgta atcatcccga ttttatcagc aacgcgctcc atgaggactt attcagaggt
    18061 tccctgggcg ttccacagga agaaagcaag aggaacggcg tgcatgacgt acccgcactg
    18121 acacccgcaa acagcgtctc cgtcctggat acccgctgga atcctttctc tttcgggtcg
    18181 ccccggtcgt tctgtccacc cggcatgctt tgttatattg tctgggctcg caacttcagc
    18241 cataataatc gttttgcgac ccgcaccgct acctggctac ctgctacctg ggtgaggagc
    18301 ggtgtttttg tgtgggatat tgttgaacga gggagttagt gttcatggga acatttagag
    18361 atttcgaagc gcccgtattc aagaacctga ccagcgctat ccgcgcgctg gcgatggacg
    18421 ctgtagagaa agccaagtcc gggcaccccg gcatgcccat gggcatggcg gaaatagccg
    18481 aggtattatg gattcatcat ttgcgccaca accctgcgaa tccgaaatgg ccagaccggg
    18541 accggttcat actgtccaac gggcacgggt cgatgctgat ttacgccctg ctgcacctta
    18601 ccggctacga cttgccaatg gaggaaatca agcgcttcag gcagcttcat tctaagactc
    18661 cgggtcatcc cgagtatggc tatacgcctg gcgtcgaaac cactacaggg cctctgggtc
    18721 aaggcctcgc gaacgcggta gggatggcgc ttgccgaaaa actactcgca tcgcagttca
    18781 accgccctgg cttcgatatc gtcaatcatt acacgtatgt tttcctgggg gacggctgcc
    18841 tgatggaagg aatatcccat gaggcgtgct ccctggcagg gacccttggc ctgggcaagt
    18901 tgatctgctt ctatgacgac aacggcatct cgatagatgg tcatgtggaa ggatggttca
    18961 ccgacgatac tcccaaacgt ttcgaagcgt acggatggca cgtcgtaccc aacgttaacg
    19021 ggcatgaccc ggttgccgta gaggcggcca tagaagagtc gaagcggata accgataggc
    19081 cgtcgatgat ctgttgcaag acggtgatcg ggctaggcgc ccccaacaag gcaaatacgc
    19141 atgaggtcca cggcgccgcc ttgggagagg cggaaatcgc ggcggctcgc ccgcatatcg
    19201 ggtggtcaca ccttccgttt gagattccgg atgacattta ttcgatgtgg gatgcccgcg
    19261 agaaaggcaa aaagctggaa gatgactgga atcgaatgtt tgcggagtat gcaggacagt
    19321 accccgccga agctgcggag ttcaagcgcc gtatatccgg cgaactgccg gatggatggc
    19381 acgaacacgc ggagggcttg atcgcacgcg tcaacgccaa ggcggaaacg attgcgagcc
    19441 gcaaggcgtc gcaaaacgca attgaaggat tggcgcctgt cttacccgaa ctggtcgggg
    19501 gttccgcgga cctggcgggc tctaacctta ccctgtggtc cggctccaag ccgatcgacc
    19561 ggtcagacgg cggcaactac atttattacg gggtccgcga gttcggcatg agcgccataa
    19621 tgaatgggct ttcgctgcat ggcggcatta ttccatacgg cggcacgttc ctcatgtttt
    19681 cggaatatgc gcgcaatgca ttgcgaatgg ctgccctgat gaaaattcgc aatctttttg
    19741 tgtttaccca tgattcgatc ggcctcggtg aggatggtcc tacgcatcag ccagtcgagc
    19801 agacggcaac gctgcgttat attccgaaca tggatgtgtg gcggccatgc gatacggtgg
    19861 aatcaacggt cgcatgggca cgcgcgatcg agcgccttga cggcccttcc accctgattt
    19921 tcagccgcca gaatcttcct ttccagaaga gggatgcgca aacaatcgaa ctgatcaata
    19981 agggtggata tattctgtct gaagctgcgg acggcgctcc gcaagcggta atcattgcga
    20041 cgggctccga ggtttcgctg gcaatgatgg ctcagaaggc actggcggag agcggcgttc
    20101 acgttcgtgt ggtctcgatg ccctgcacga acctcttcga tcggcaggat caggaataca
    20161 aggacaccgt gctccccagg gggcctggac gcgtagccgt tgaagccggt attaccgatt
    20221 actggcgaaa atatgtcggg ctcgaaggag cggtcattgg aatggatacc tttggcgagt
    20281 ctgcgcctgc aaatgacctg ttcagctact ttggcttcac tgtcgagaat gtggtaaagg
    20341 cggtaaacag cgttattcaa tccaatcacc gggtttcata acgccagtgc ttcctgacat
    20401 ttcatcgatc caggtttatt ttcattcaag gagttaactc atgacaatca aggtgggcat
    20461 caatgggttt ggccgcatcg gaagaatggt attccgagca gcggtacaga attttccgga
    20521 catcgaagtc gttgctatca acgatctact tgagcctgac tacttggcat acatgctgga
    20581 gcacgactcc gtgcacggac gtttcaaggg tgacgtttcg gtggaagata atactttggt
    20641 cgtcaatggg cgcaggatac gcctcactgc gatcaaggat cccgcggagt tgaagtgggg
    20701 tgaggtgggc gccgatgttg tcgttgaatc gacgggcctc ttcctgacga aagagagctg
    20761 cgaaaaacat atcgccgcgg gcgcgaaaaa ggtcatcatg tccgcaccgt cgaaggatga
    20821 cacgcccatg ttcgtgttcg gtgtgaacga ttctacgtat gcgggagagc ccatcatatc
    20881 caacgcatct tgcaccacga attgtctggc ccccatagcc aaggttctga atgacacctg
    20941 gggcatcaag cgcgggctca tgacaaccgt tcatgccgcc actgcgacgc agaagacggt
    21001 cgatggccca tcgaacaagg actggcgcgg aggccgtggt attcttgaaa atatcatccc
    21061 ctcatcgacc ggggcagcaa aggccgttgg caaagtgatc cctgaactga ataaaaaact
    21121 gacaggaatg gcttttcgcg ttccaacatc cgatgtttcg gtcgtggatc tgaccgtcga
    21181 actcgaaaag gatgcaacct atgaagaaat ttgcgccgca atgaaatccg cttccgaggg
    21241 gtcgatgaaa ggagtcattg ggtacaccga cgagaaagtg gtttccactg attttcgcgg
    21301 ggatagctgc acctccatct tcgatgccga ggcaggaatg gctctagatg acagcttcgt
    21361 caaggtggtt tcctggtatg acaatgaatg gggttattcg agcaaaatcc tcgaaatggt
    21421 tcgtgtcgta gctggtaaat agcccatagg aagttgcgga acgttttgcc ttccgcaact
    21481 tccccctccc ccatctcgtt tcaacggttt cgtggcttgc ccgtgcatca tcggaaccaa
    21541 aattggagtt atccgtgtcc atcattaaag tcaccgacct cgaccttaaa ggcaaacgcg
    21601 tctttattcg cgcagatctt aatgtaccta tcaaggacgg cgaggttact tcggatgctc
    21661 gtattgccgc ttccatggct acgatcaatt tctgcctgga acaggggggg cgggtaatgg
    21721 taacctctca cctcggccgt cccgaggaag gcgtctggtc ggaggaaaat tcgctcaagc
    21781 cggtggcgga agatatttct gcacgtctcg gaaagcgggt gcgcctgatc aagaattggg
    21841 ttgaaggtgg cttcgaggtc gacgagggcg aactggtcgt tctggaaaat tgccgtttca
    21901 acaaaggcga gaagaaagat ctcgatgaaa ccgcaagaaa atacgccagc ctttgcgatg
    21961 tatttgtcat ggatgcgttc ggcacggcgc acagggccga ggcttccacc tacggtattg
    22021 cgaaatatgc tcccgttgca tgcgccggta tcctcctgac ggaggaactc gaggccctca
    22081 ccaaggcgtt gctgaatcca gcgcgcccga tggttgcgat tgtcgggggg tccaaggtct
    22141 cgaccaagct caccgtactc gaatcgctgt cggataaagt ggatcagctt gtggtcggcg
    22201 gtggtattgc caacaccttc ctgaaagcgg taggtaaaaa cgttggaaaa tctttgtgtg
    22261 agcctgatct tgtgcccacc gcaaggaacc tgatggaaaa gatggcaaag cgcaatgcat
    22321 cgattccgat tgccgttgac gtcgtcgtcg gaaagaagtt cgatgcgaac gagccagcgg
    22381 tggtaaagga ttccgatgac gtggctgatg atgacatgat ctttgatata ggcccgaaaa
    22441 gcgctcagac gctggccgat atcatcatga aagcaggcac tgtagtctgg aacggtccgg
    22501 tcggcgtgtt tgaattcgac cagtttggga acgggaccaa aaccatcgct accgccattg
    22561 cgaacaccga tgccttcacc ttggcaggtg gcggagacac catcgcggcc atccagaaat
    22621 atggcatata tgacaaagtg tcatatattt ccacggcggg cggggcgttt ctcgagttcc
    22681 tggaaggaaa gaaattaccc gcagtcgaaa tactcgagca aagaggggac tgattgctgt
    22741 tatgcgggga ttgaatttgg caccggaaaa atgattcgaa gaacaaaaat tgtcgcaacg
    22801 ctcggacccg cctgccggga cccaaaggtt ctggagcgga tgattaacgc cggcgtcgac
    22861 gttgtgcgca tcaacttctc ccatggaaca agggaagagc atattgaatt cgcggagctg
    22921 acgcgatcac tcgctcgttc tgccgggcac gccgtaggag tcctggcgga cttgcaggga
    22981 cctaaaatcc gtatcggtaa attcgaagaa ggcaagattg tactcaagac cggtgacaaa
    23041 ttcgtactgg atgctgaatg taatctcggc aatcaggagc gtgttggtct cgactataag
    23101 gagttgccgg gagatgtcga aaccggtgca acgctgatcc ttgatgatgg aaggatcgtc
    23161 ctgggcgttt ccgacgtcag gggacaagag attcactgtg ttgttgagca gggtggggcg
    23221 ctatccaaca acaagggcat caatcggaaa ggcggagggc tcagtgcgcc tgcgctgacc
    23281 agcaaggatg tagaggatat ccggacagcc gcggcgctga aagcagatta cctggcggta
    23341 tcttttcccc gttcgggaga ggacatgcgc tgggccaggg aggtcatgca tgaagctggg
    23401 gggaaaagtc tcctgatggc gaaggtggag cgctcggagg cgattctggc actggacgat
    23461 atcttgcaat cttcggatgc catcatggtc gcgcgcggag accttgcggt tgaggttggg
    23521 gacgcggtcg tgccggcatt gcagaagcgt atgatccgaa cggcacgtgc caacaataaa
    23581 ctggtagtca ctgctaccca gatgatggaa tcgatgatca acagtccgat ccccactcgt
    23641 gcagaggtat ctgatgtagc caatgccgtg ctcgatggaa cggatgcggt catgttgtcg
    23701 gcggagtctg cgacaggaca atatccagtg gagtcggttg ccgcgatggc cagagtttgc
    23761 cttgaggctg aaaaagaata ccttgcgagc aacgaacttc gacggctcca gggtggtctt
    23821 ccggaaacca tagaagaagc aattgcgcga gctaccatgt ttacctccgg tggccttaaa
    23881 attcgcgcga tagcggcgct gacgcaaagc ggaaggacgg tattgctgat gtcgcgaaga
    23941 agttccaacg tgccgatttt tgccctgagc ccacaggaag atacccgtcg taaaatgacg
    24001 ctgttccggg gggtatatcc cataaagttc gggggtggct ccaatgatcc cgagatcatc
    24061 ctgaatcacg ccgaaaatga actgctcaaa cggggtgccg tgaggaatgg cgacctgatc
    24121 atcatgacga tcggtgaacc cgttggcaag gccggcggca caaacaccat gaaaatcgta
    24181 aaagtaggcg agtacagaaa ggcctgagta agtccacgcg agacttgttc agaggttcct
    24241 taagaactca caacagattc taacttggag gaggctcaaa tggcacttgt atcactgcgt
    24301 caacttttgg accatgcagc ggaaaatggc tatggcctgc ccgcattcaa cgtcaataac
    24361 ctggaacaga tccatgccat catgcaggcg gcggatgaat gcaacagccc cgtaatcatg
    24421 caaggttcgg ctggggcgcg taaatacgcc ggcgaggctt ttttacgtca ccttatcgag
    24481 gcggcagttg aatcctaccc gcacattcca attgtgatgc atcaggatca tggcgcgtct
    24541 ccctcggtgt gcgtcaatgc catccgaagc ggcttttcca gcgtcatgat ggatgggtca
    24601 ctgcaggcgg atgcgaagac accgtcatcg tacgaatata atgtcgatgt gacgggaaag
    24661 gtggttgaaa tggctcatgc cgttggtgtt tcggttgaag gcgaactggg ttgtctcgga
    24721 tcgctcgagt ccggcatggg agaagctgaa gacggacatg gtgcggaagg caagctctcc
    24781 cacgatcaac tattaaccga tccggaacag gcggcggact tcgtcaagca gacgggcgtg
    24841 gatgccctgg ctatcgccat cggcacctcg cacggcgctt ataaatttac ccgcaagccg
    24901 acgggagaca tactcgcgat tgaccgggta aaagaaatac atgaacgcat tcccaatacg
    24961 cacctggtca tgcacggttc cagttcggtt ccgcaggaat ggctggacat cattcgcgaa
    25021 tatggcgggg aaatgaaaga aacctacggc gttccggtgg aagagatcca ggaaggaatc
    25081 aaatacgggg tgcgcaaaat aaatattgac actgatatcc ggttggcaat gaccggagca
    25141 ataagaaagc atctggcgaa gaataagagc gagttcgatc cgcgcaagta cctcaaggat
    25201 gccactgctg cggcgaagga gatttgcaag gcgcgcttcg aagccttcgg cagcgcgggc
    25261 caggcaagca agataaagcc tctcaatctc gaaagcatgg cgaacaagta tgccaagggt
    25321 gaattgaagg cagtgatcag ataagaacca acgaacggca cagtgagcgt tctcactgtg
    25381 cccgccttgt tgtgagacaa ggccacagtt gagcggccat tggcgtcggc cagacctgcg
    25441 cgttgcttta tgtttaacct gattcccggt ggaaacgggg cctcccgtat tccttcccgt
    25501 gttccttatt ccttgctcag cctcgcgtat actattttgc cgctgccagt tcggtcagta
    25561 gcgtttcctg cgcgcaaagc cttttccccc ggcggccggt tctaagacgg tagtgcccat
    25621 ctccattcat ttcccaggct tggcgattat ccttgagata aatcgtaaga ccttccgaaa
    25681 gcacgcgctt ttttaacttt tcctcaagca cgggaaaaca tacttcaatt cggcggaaaa
    25741 aattgcggtc catccaatcg gcgctggaga ggtaaatggc gtgcgcgccc cccttccgga
    25801 aatagaatat ccggctgtgt tccaggaaac ggcctattac cgaacgtacc cgtatatttt
    25861 cggacaagcc ctttacaccg gggcgcagtg cgcatacacc ccgtatgatg agatcgattc
    25921 ttactccggc cgcggaagcg acgtaaagcg ccttgattat ttccggctca agcagggcat
    25981 tcattttggc gataatgcgg gcgggctttc ctgccttggc attttcaatc tcgttatgga
    26041 tggcggcaag catctggtta tgcaagctga acggggattg ccacaggtgc ttcagctttc
    26101 cggccttgcc aagccccgtc aactgcatga acacctcgtt cacatcaccg caaaaatcct
    26161 cattgcaact aaacaggccg aaatcagtgt actgccgggc ggtgcttgca tggtagttgc
    26221 cagtcgcaac atggatataa cggcgaagct tcccttcttc ccggcgtacc accatggcca
    26281 tttttgcgtg cgttttatgg cccactaccc cataaacgac atgggctccg acttcctcca
    26341 ggcggcttgc ccagttgatg tttgcctcct cgtcaaaacg tgccagtaac tccaccacta
    26401 ctgttacttc cttgccagaa ccagccgctg caatgagcgc ctccataatc aacgagtctg
    26461 ttccggtacg gtagaccgtc tgcttgatgg ccacgacgtc cgggtcgacc gctgcctgct
    26521 ggataaagtg aatgaccggc tgaaaggatt gatagggatg atgcagaaga atgtcattct
    26581 tgcgtatcat ctggaagata tcgactgcct ttttcctttg gagggtttgt ggcaggcctg
    26641 gaataaatgg cgggtatttc aggtggggac ggtcgatcca gtccgggacc tgcatgagcc
    26701 gcacgagatt tacaggccct tccacctgat aaaaatcttc ctgcgagagc ccaagttgct
    26761 cgaggaggaa atcggacata tgcttggggc agttgtccgc aacttccagc cggactgcat
    26821 cgccaaagtg gcgttgcggc agttcaccct gaagcgcgat tcgtaaattc ttgacctctt
    26881 cctcatccac gaaaagctcg ctgttgcgcg tcgcacgaaa ttgatagcag ccaatcacat
    26941 tcatgccgga aaacagctca cccacgtggg catggagaat ggacgagaga aatacgaaac
    27001 tgtaatcgct gtcgctgatt tcacgcggaa gcgggatcag gcgcggcaat acacgtgggg
    27061 cttgtacgat agccgtgccc gagcttctcc caaatgcatc cctgccttcg agctcgacag
    27121 caaaattcaa gcttttattc aggacgcgtg gaaaaggatg ggcgatatca agcccgattg
    27181 gagtcaatac aggcatcaac tcccgaaaga aaaaggcgcg gatccattcc tgctgagcct
    27241 cgttccaggt tgatcgacgc aaaaaccgta taccttcctc cgcaagcttc ggcagaattt
    27301 gctgattgag taactggtat tgaagcgcta ccagtgaatg cgtctgctcg gagacgcgct
    27361 tgaatacctg gtttggaagc attccatccg gtccaaaccc cggtgtctcg agcttgatgt
    27421 gctccttcaa tcccgctatt cttacttcga aaaactcatc aagattgctg ctgacaatgc
    27481 acaggaatct cagtcgctct aaaaggggag tatccttgtt ttccgcctgg gccaaaacac
    27541 ggcgattgaa ctcgatctgc ccgagttcac ggttgagaaa ctggttaccg gtttgggcgc
    27601 ctgacattaa agtgttactt cacccgatgc ggaagttaag aggcgattta gtaaccgtca
    27661 ttcctcctgc ttctcagggg gaacgtaccc cgcaggctgc tcagccccct ttccgaaaaa
    27721 gtacgcctcg agctgctcag ccagatactt tcgcgccttg atgtccgaaa ggttcaggcg
    27781 caactcgtta accagcatgg tttggtgctt gatccaaccc ccccaggctt ctttcgatac
    27841 attatcgaag atgcgcttcc ccagctcgcc tggatagggt gcatagtcca gccctgccat
    27901 ttcacgcccc agcttgatgc aatgcacaac cctcgccatc gatccctcca gtatcaagtc
    27961 ctcaagttca aactgaccca acatttgccc gccctactca gtaataacga ccgggatgat
    28021 cgcacttagg tgcgtaaagc cgcgcctcct cacagcttct tcagaaatac cttggagcgc
    28081 cggctgtaat tgtatagccc ttgcctggcc ttcggtagct cgtccacgct tacttcccgg
    28141 aaaccacgtt cgacaaacca atgagcagtt ctggtggtca atacgaacag gttcgtgttc
    28201 ccctgagccc tcgcccgctt ttcgatattc ttgagcaagg cttctccaca accggaactc
    28261 cgatagtccg gatgcaccgt gaggcacgcc agttccgcag cctgttcatc agggaatgga
    28321 tacagcgcgg cgcacccgac tattattttg tcgtgctcga tcacaacgaa atggtcgatc
    28381 tcaatttcga gcaattcacg gctgcgtcgc accagggttc cgtccgcttc aagcggctct
    28441 atcaattgca gaatagagcc gacatcctcg atctttgcct cccgcaaggt ctgtaacggg
    28501 ccttcggata tcatcgttcc gatgccggtg tgggtgaaca gttcctgaag caaggcgcca
    28561 tccacatgtc tgctaatgag atgtatccgg ggcacacccc cctcacaagc acgcatagca
    28621 aacggcagat acaattgaat atcgccgggc tccgtggatt ggccccggaa ctccttcagc
    28681 atcgctctac cttctgccag tgtcaattcg cgtacaagcg tcatgccgga actcgaagca
    28741 tcttcctttt ctatgccttg ggtatccatc aaaaagatga gcttctctgc cttgagcgcg
    28801 atggctgtct cagcggccac gtcctccagc gtaagattaa atatctcccc ggttggcgaa
    28861 tagccgagag gagaaagaag caccagttca ccctgctcca ggcgactgcg gactgccatc
    28921 gcgtcgatct ttcgcacctc tcccgtatgc atcagatcga ctccgtctat cacgcccact
    28981 ggacgagccg tgacaaaatt tccgctcgct acccggatgg cggcattcgc catgggcgaa
    29041 ttgggaaggc ccatggataa cagcgattca atttcaaccc ggattcgtcc tatagattcc
    29101 ttgacgcact gcagcgcctc actatctgtg acgcgcatcc ctttcacgta tagcgtctga
    29161 agatggcgtt cccccagccg tgattcgatt tgcggccgcg cgccatgcac caggaccagg
    29221 cggacaccta cacttgccaa aaggttgagg tcatggacaa attcgacgaa ttttccgtca
    29281 gcgattacct ccccgccgaa agcgataaca aagattttgt ttcgaaaagc gttgatgtaa
    29341 ggcgcgacgg aacgaaacca ggcgacaaaa tccatatctc gatcatctgt taattttttt
    29401 cggctcatga agccgattat gctatatgaa tttcatggca tacaaggaag acatgccgct
    29461 caaaaatcgc cccacactgc ctgcgcaacg gcaattgccg tcaatcccgc agtttcggtg
    29521 cggagggtac gcgggccaag gtttagcggg gcaaactcag ccacggcggc ggcagcctcc
    29581 tcttcccggg tcaggccacc ttcaggtccg accaggaagg tcacggagga aacggaagga
    29641 ccttcagtga aacaattcaa actctcttcg gcataaggcg agagcataaa gcgcaaattg
    29701 cgatggtcgg agccaagtct gcttttctcg tccatttttg agccaagcca gctgggaagt
    29761 gtggccagcg gcgagatttg gggaatccgg ttcctgccgc attgttcaca tgcggatatg
    29821 gcaacctgcc gccaatgtgc catgcgttta tcgactcgct catctgaaag tttcgttgta
    29881 cacagctttg tggcaagaac ctggatgtcg ctcactccca actcgactgc cttttgcacg
    29941 atccagtcca ttttttctgc cgcgcaaacc gcctgagcaa ggcttattac aagaggagat
    30001 tctcgctcga tgcccagata ctgctcaaca gcaaccgtag cgccatcccg ctgcgtccgt
    30061 tggattctcg cccgaaattc cccacctttc ccgttgaata gaataagttg gtcccctggc
    30121 tggagtctca aaaccctcac cacatggcga gttgcgcttc ctgacaattg gataacactc
    30181 tcggcagaga tttcgtcaga atagtaaata cggggaaacg ccattgtttc agtttcagta
    30241 gcaactgcaa tcaggcgctt ctgacatggt aagatgtttc aagccgagtc tattagcaga
    30301 gtttctcatt cgatcggcga ctttcggcaa ttatatctcc cttggcatct gcttaatacc
    30361 tccttaacct tgttccgcta atcatcgttt ttccacctga ccgaaacgcc ttttatcgaa
    30421 aaactgaatc tatcaatttc tttctggagg agagtccatg gcaagtctgg aaacacctgt
    30481 ttgtaacttt ggttggaaag ccgttgattt tgatctacca ggggtggatg gaaagcggta
    30541 caaccttgca tctgtaaaag gcgaaaacgg attgctggta atgtttatct gcaatcactg
    30601 cccttatgtc aaggctgttc gagatcggat catccgtgat gtcaccgatt tacaatcgta
    30661 tggcattggc gcaattgcaa tcatgtcaaa tgacccctcc gattacccgg aggattcttt
    30721 cgataacatg attcgcgtgg cccgcgaata tcactatccc tttccctatg tatgggatgg
    30781 aacgcagcag attgcaaaag catatggggc ggtgtgtacg ccggacttct tcggcttcaa
    30841 tggcgacctc gaattgcagt atcgaggccg gctcgacgcc tcccgcaagg aagccgcccc
    30901 gcctcatgca caccgcgacc tgttcgaagg tatggtgcag gtcgcgacga caggacgtgg
    30961 accggaaaac cagattccca gcattggctg ttcaataaaa tggcgagcag aataggaagt
    31021 gcttaacgac cttattacca ttgtaaaggc ggtcgcacag caggaaatca tgcctcgcta
    31081 catgagggtt gcgcgtcagc gtaaggcgga tggcagtctt ttcacccaag cagatgtgat
    31141 tgctcagaac gcgttgtccc gcgaattgac gaagctatac ccaggacctg tcgtgggtga
    31201 ggaaatggcc gaggaggagc aaaaagaacg ctggcatgct ggatccgaag ggctgtggtg
    31261 cctcgacccc atcgacggca cgtcaaactt tgtgaacggc ctgccgtatt ttgccgtttc
    31321 cgtagctttt atgcaaaaag gcaaaagcgt gctcggggct gtatataacc cggtcgcgga
    31381 tgaaatgttt tacgccgaac aaggaaaggg agcatacctt aacggtgaac gattgcctat
    31441 cagtaccagg gccgcgactc tgcgtggcgc tatagccaac gttgatctca aacggctggg
    31501 gcagaagctt gcggaagcgg tttgctcggc gcggcctttt tcgtcgcagc gtaattatgg
    31561 atcttgctcg ctcgagtggt gctataccgc agccgggcgt tttgatttat acctccacgg
    31621 cggacaaaag ctatgggatt atgccgccgg ctgtcttatc ctggaggaa
//
LOCUS       DASSHJ010000119         7814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_317573, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000119 DASSHJ010000000
VERSION     DASSHJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317573"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1424
                     /locus_tag="VFI43_04840"
     CDS             87..1424
                     /locus_tag="VFI43_04840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7061487.1"
                     /translation="MDDKDDLIGKTIHGSYLIISLIGEGGMGRVYLAENVEVREKKYA
                     IKVLKRELTANPKFMNQFFEEATHQAQLDHSNIVQMENYFKEGEDYFLVLSFVEGRSL
                     AEIIDSLHGPMPKKQAAQIFKEVLRALNCAHEKAILHRDIKASNVMVDNSGRALLTDF
                     GIARQAGDEQAASLGQVIGTPEYMSPEQFIDSDKVDHRSDVYSAGILLFEMLTGQLPF
                     KGSQDELREKHLSAPVPNPRTINPKIKKCLAEAVVKATQKEPDARFQGCIAFLKAVER
                     CERGRRWEIAALGLSVVIAAGAWYMTQAENEKAARTLAASSVSNYASLCREAEALERK
                     ELGLRTAQGIGDSGMAEAFQKEINAHKIKISKFFGEYSNALDELSKYQGGMVHKVLKE
                     SVHEEEANRTPDPGRQAERERFERLTGENFDQRSSTGKGPTVETMLHNCPRNS"
     gene            1565..1957
                     /locus_tag="VFI43_04845"
     CDS             1565..1957
                     /locus_tag="VFI43_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HET7061488.1"
                     /translation="MELEIKRTDFTDNSTIGELSANSEFECYTLEDKVRPVKMAGKTA
                     IPPGRYEVIINFSQRFGRELPLLLNVPDFEGVRIHPGNTNEDTEGCILVGETKRTNFV
                     GDSKPAFERLFPKLKAAYGKEKIYLEIG"
     gene            1967..2476
                     /locus_tag="VFI43_04850"
     CDS             1967..2476
                     /locus_tag="VFI43_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061489.1"
                     /translation="MKPSILAGIAFVFFSIEACAEAVPFRQEKTYRLPSGKRVATVFA
                     ERWCKPGECGVADSGVWGVDGGIPRFVTAVFRIDIDGRAFSIPEKFYKDLTNTYFLDV
                     LEQNGKVVVELRGGEAAGAYTAHFILGGMCGFERRVCGEACNEIWEQTTWHNSFAYDL
                     EPECRSGIK"
     gene            2523..3290
                     /locus_tag="VFI43_04855"
     CDS             2523..3290
                     /locus_tag="VFI43_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HET7061490.1"
                     /translation="MRDNFIPLIMDALSRAFRDLFQSQVLWFMVWPIVAASLLWIVLD
                     MTFSSLFAEWVASTLDSVGIRDGLEDLHPRWAAHAVQALAHLILFTPLVFVTAVIMTA
                     LFSMPALVSLVSERDYPQLKHEKGGGTVGSLVNVLLGLPIFIAIWMITGPLWLAGAGA
                     ILPFIAAAYLNQRMFRYDALLEHASFDELETLFFIHRVPLWILGLVTGLIQFIPVFNL
                     FAPVFTGLAFAHFSLARLDEFRQHPREVLLHASQSEQ"
     gene            3407..5266
                     /locus_tag="VFI43_04860"
     CDS             3407..5266
                     /locus_tag="VFI43_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061491.1"
                     /translation="MSIFTPAAAQNRKEMFSLDGTSFGKSAKTVRKAVFPIAGLGTFF
                     VLLAGATTMAAGANSAFAESGAVGLSLNLADSRNKHVSRPDQPASRQADEQAAPRLLN
                     LGSHTFPVSTRNRLAQTYMNQGLNLSYAFNHAEAGRSFKEATRLDPSLAMAYWGQALV
                     LGPNINAPMDPAAEQTALELVRKAASLSAHASPREGALIHALEKRYTGNGEDRKANDN
                     AYAKAMREAHRQFPDDLDIAMLYVESVMDLDSWGYWMPDGYPRNETAQIVALTESVLR
                     RNPRHPGATHMYIHLVEPTSTPERAEKAADTLLTLMPDAGHLIHMSSHIYQRVGRYAD
                     AIKSNELAIAADKNYLAHQHAQGIYPLVYTPHNMHFLWWAATADGQSRLAMESARKAA
                     AVIDDQTLAQVPATAIFRVVPYWAYARFGKWNEIFQEPEPPGSNGFLKGGWHYARGLA
                     FVATRQLPQAEQELSALREILKNDPLDWPLLSRNTARAVLSIAPEVLAGEIANAKGQF
                     DVAIAHLDRAVRLNDDLIYTEPAEWQSPPRLTLGAVLLEAGRPAEAEAVYWEDLKRNR
                     NNGWALFGLLQSLRDQTKEDRAGQVEARFKKAWARADVDLRASRFGCISAPQR"
     gene            5447..6019
                     /locus_tag="VFI43_04865"
     CDS             5447..6019
                     /locus_tag="VFI43_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7061492.1"
                     /translation="MQTEESPEDHPLVFTIGHSMRPLDEFIGMLKKNGVERVLDVRTV
                     PRSRRNPQFDKSVLPESLPEAGIKYTHLPELGGLRHTKRESINKGWRNESFRGYADYM
                     QTPEFEAGVREVLGLAQNERCALMCAEAVPWRCHRSLIADALLLRGARVEHIIHSNER
                     KLHVMTPWAQTDGLKIFYPPSEDTEDSDNK"
     gene            6113..6562
                     /locus_tag="VFI43_04870"
     CDS             6113..6562
                     /locus_tag="VFI43_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061493.1"
                     /translation="MKIFLLIIASLASLTGLAFQPVLAYQPALPGQAGAGKNPASQRT
                     GQAGQPGSPELNNPLSRPQNMQSKPWEARPWEQAQEELRGEELKRSDPRKMEEGRRVG
                     RKARKERAARLKEKGQAVSYEDRVKQRQTRWQRHQLQSDQYSTRMSQ"
     gene            6764..7798
                     /locus_tag="VFI43_04875"
     CDS             6764..7798
                     /locus_tag="VFI43_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET7061494.1"
                     /translation="MVRDLLGQQARLMEEKHRDMLKDMHESLANQGDRLSDALGKNSE
                     QLRGTLEARLDVISGKVAERVEEGFRKTNETFTNVMARLATIDEAQKKIDSLTTNMVS
                     LQELLGDKRSRGAFGEVQLEALVRNILPPAAYTMQYTLSNGHRVDCVLRLPPPTGMVA
                     VDSKFPLENFHRMFDQAASEVDRSAAQKQFKADVKKHIDDIAGKYVVPPETCDGAVMF
                     VPAEAIFAEIHAYHYDLVDYAMQKQVWIVSPTTLMAVLNTARAVLKDLETREQVHIIK
                     NELSRLGKDFVRFDERMKKLADHIRQANQDVEEVHISSRKISQRFARIDAVELDAAQA
                     DSPALTPGGD"
BASE COUNT         1888 a         1967 c         2255 g         1704 t
ORIGIN      
        1 aaccgatcag gaattggaga tctgcgcttt agttccggac gaagccaagc gccatgatga
       61 gagatgatca ataaggagtt cgcggagtgg acgataagga tgatctgatc ggcaaaacga
      121 ttcacggcag ctatctcatc atctccctga tcggagaagg aggaatgggc cgcgtttacc
      181 ttgcggagaa cgtcgaggtg cgtgaaaaga agtacgccat caaggtgctg aagcgggagt
      241 tgaccgcgaa ccccaagttc atgaatcaat ttttcgagga ggccacgcac caggctcagc
      301 tcgatcattc caacatcgtt cagatggaga actatttcaa ggaaggcgag gattatttcc
      361 tggtgctgag ctttgtcgaa ggcaggtcac tggccgaaat aatcgattct ttgcacggtc
      421 ccatgcctaa gaaacaagct gcgcagatat tcaaggaggt cttgcgagca ctcaattgcg
      481 cacatgaaaa agccattctg catagggata taaaggcgtc caacgtcatg gtcgataact
      541 cggggagagc gctcctgacc gatttcggca ttgcgcggca ggccggcgac gagcaggctg
      601 ccagcttggg gcaggtgatt ggaacgccgg agtacatgag ccctgagcaa ttcatcgact
      661 cggataaggt tgatcaccgg tctgacgtat actccgctgg aattctcctg tttgagatgc
      721 ttacggggca gttgccattt aaaggttctc aggatgaact cagggaaaaa caccttagtg
      781 ctcctgttcc caatccccgc accatcaacc caaaaataaa aaaatgcctc gcggaagcgg
      841 ttgtaaaagc gacgcaaaaa gagcctgatg cccgttttca gggctgcatc gcctttctga
      901 aagcagtaga gcgttgcgag cgcggaagaa gatgggaaat tgcggcactg ggcctttctg
      961 ttgtgattgc tgcaggcgcc tggtatatga cccaggcaga gaatgaaaag gcagcacgca
     1021 ccttggcggc atcatcggtg agtaactatg catcgttgtg cagagaagct gaagcgcttg
     1081 aaaggaaaga gctgggtctt cgcactgctc agggaatagg cgattccggc atggcggaag
     1141 cgtttcaaaa agaaatcaac gcccacaaga taaaaatcag caagtttttc ggtgagtata
     1201 gcaacgcgct ggacgagcta tccaaatatc aagggggcat ggtccataag gtgttgaagg
     1261 aatccgttca cgaagaggag gcaaacagga cgcccgatcc tggacgccag gcggagcgcg
     1321 aacgattcga gcgcctcacg ggcgagaatt ttgaccagcg cagcagtact ggcaaggggc
     1381 caacggtaga aacgatgcta cacaactgcc cccgcaacag ttagcttata tgacgttagg
     1441 tgtcccaggt tgggcatcgg cgaaactgca ataagcaatt taacgtttgt tttaagacat
     1501 gcaagggcca agggtctaaa aatatgacgc aagcgattaa gggatcaata agggagggat
     1561 caacatggag ttggagatta aaaggaccga ttttactgac aacagcacaa ttggcgagtt
     1621 gtcggcaaat agcgagtttg agtgctacac tttagaggac aaggtcaggc cagtcaaaat
     1681 ggcgggaaaa acagcaatac cacctggacg gtacgaggtg attatcaatt tctcgcagcg
     1741 gttcgggcgt gagcttcctc ttctgctcaa cgtaccggat ttcgaaggtg tacgaataca
     1801 tccgggaaat acgaatgaag atactgaggg ctgtattttg gtaggggaaa ccaaaaggac
     1861 aaattttgta ggtgatagta aaccggcttt tgagcggtta tttccgaaat tgaaggcggc
     1921 atacggaaaa gaaaaaatct acctggagat aggctgaagc cagggaatga aaccttccat
     1981 acttgcaggt attgctttcg tctttttcag tattgaagct tgtgcagagg ccgtcccatt
     2041 cagacaggaa aaaacctata ggttgccatc gggcaaacgg gttgcaactg tcttcgcgga
     2101 gcgttggtgc aagccgggcg agtgtggcgt ggcggactcc ggcgtctggg gagtcgacgg
     2161 cggaattcct cgtttcgtca ccgctgtttt tcgtattgat atcgacggga gggcattttc
     2221 cattcctgaa aagttttata aggaccttac caacacgtat ttcctggatg tgctggaaca
     2281 gaatgggaag gttgtcgtcg aactgagagg aggagaggca gcgggcgcat atacagccca
     2341 tttcatactt ggcggaatgt gtggattcga acggagagtc tgcggcgaag catgtaatga
     2401 aatatgggag caaacaactt ggcacaactc atttgcttat gatctcgagc cggaatgcag
     2461 gagtggaatc aagtgatccg gccgtaccgg aaggggcgtt aacccaatac acagagtgaa
     2521 ttttgcggga taatttcatt cctctcatca tggacgccct atcgcgggcg ttccgggacc
     2581 tgttccagag ccaggtttta tggttcatgg tatggccgat tgtggcggcg agcctcttgt
     2641 ggattgtcct ggatatgacc ttctcgagtt tgtttgccga gtgggtcgca agcacgctgg
     2701 actccgtcgg tattcgggac gggctggaag atctgcatcc cagatgggct gcccatgcgg
     2761 tacaggccct tgcccatctg atcctgttca cacctctcgt ttttgttacg gcagtcatca
     2821 tgacagcctt gttttcgatg cctgcgctgg tcagtctggt gagtgaacga gactacccgc
     2881 agcttaaaca cgagaaaggg ggagggacgg tcggcagctt ggtcaatgtt cttcttggct
     2941 tgccaatttt catcgcgatc tggatgatta caggcccctt atggctggcg ggggcaggag
     3001 cgattcttcc ctttattgct gctgcatatc tgaaccagcg gatgttccgg tatgacgcgc
     3061 tgcttgagca cgccagcttc gatgaattgg aaacgctctt tttcatccat cgggtacctc
     3121 tttggattct gggcctcgtg accggtctga tacaattcat acccgttttc aatctcttcg
     3181 caccggtttt caccggcttg gcttttgcgc attttagctt ggcgcgcctc gacgaattca
     3241 ggcagcatcc acgtgaggtg ttgttgcatg ccagccagtc ggaacagtaa catcaacact
     3301 tggctttgcc cggactgaaa agcgggcgag tccaatcctc ggtagatcgc atggccgtct
     3361 gttcaggttt atccgcgcag tcccgcgcaa tcaattaaga ctacctatga gcattttcac
     3421 tcccgccgct gcccagaacc ggaaagaaat gttttctctg gatggcacat cgttcggaaa
     3481 gtccgcaaag acggtgcgga aagcggtatt tcccattgcc gggctgggca cttttttcgt
     3541 cctgctcgcc ggcgcaacga caatggctgc cggtgccaat agcgctttcg ccgaatccgg
     3601 cgcggtcggt ctctcactca acctggcaga ttccaggaac aagcatgtct cgcgtccgga
     3661 tcagccggcc tcccggcaag cggatgagca ggctgcgcca agactcttga atctcggctc
     3721 ccacaccttt ccggtaagca cgcgtaacag gctggcgcag acatatatga accaggggct
     3781 taacctctcc tatgccttca atcatgccga agcgggccgc tcattcaagg aggccacgcg
     3841 tctcgacccg tcgcttgcaa tggcttactg gggacaggcc ctcgtgctcg gccccaatat
     3901 caatgcaccc atggacccgg cagccgagca gacagcgctg gagttggtaa gaaaggcagc
     3961 ctctctttct gcacacgcat ccccacgcga aggcgcgttg attcacgcgc ttgagaaacg
     4021 ctacacggga aatggtgaag atcggaaagc gaacgataat gcttatgcaa aggcaatgcg
     4081 cgaggcgcat cggcagtttc ccgatgatct ggatattgcg atgctgtacg tagaatcagt
     4141 aatggatctt gattcgtggg gatattggat gccggatggg tatccccgca atgaaacggc
     4201 gcagatcgtc gcgctgacgg agtccgtgct taggcgcaat cctcgtcatc ccggcgccac
     4261 gcatatgtac atacatctcg tcgaacccac gagtacgccc gaacgtgccg agaaggcagc
     4321 cgatacactg ctgacattga tgccggatgc cggtcatctc atccatatgt catcgcacat
     4381 ttatcagcgc gtaggcaggt atgccgatgc tataaaaagc aacgaactcg ccatcgcagc
     4441 ggataagaac tacctcgcgc accagcacgc ccaagggatt tatccgctgg tttatacccc
     4501 ccacaatatg catttcctat ggtgggctgc aacggccgat gggcaaagcc ggctcgccat
     4561 ggaatccgct cgcaaggccg cggccgttat cgatgatcag acgctcgctc aggttcccgc
     4621 taccgcaata ttccgggtag tgccttactg ggcgtatgca aggtttggaa agtggaacga
     4681 gatattccag gagccggagc cgccaggcag caacgggttt ctgaaaggag gctggcatta
     4741 tgctcgcgga ctggccttcg tggcgacgag acagttaccg caggctgaac aggagttgag
     4801 cgcgttgcgt gaaatcctca aaaacgaccc gctcgactgg ccgcttctat cgagaaatac
     4861 cgcgcgtgca gtcctctcca tagcccctga agtgcttgcg ggcgagattg cgaatgccaa
     4921 ggggcaattt gacgttgcga tcgcgcacct cgaccgtgcc gttcgtctca acgatgacct
     4981 gatctatacg gagcctgccg agtggcaatc ccctccaaga ctaacgctcg gggcggttct
     5041 tctggaagcc gggcggccag ccgaagcgga agcagtctat tgggaagatt taaagcgtaa
     5101 tcgtaataac ggctgggcgc tcttcggctt gctgcagtca ctgcgcgatc aaacgaaaga
     5161 ggatcgggcg gggcaagtag aagctcgctt taaaaaagcc tgggcgcgtg ccgatgtcga
     5221 tcttcgcgct tcgagattcg ggtgcatctc agctccgcag cgataaaggc aattgttctt
     5281 tccgggaact gctgtcggtc gtttagcact gccgaggttc cttcttttac ttgcgtttca
     5341 ggttcacgag cgccccgctg tcagcctatg cgtcgccctt ttattgaggg aattgcgaag
     5401 cgagcttttc ccttccgctg ttatccgtcc tctcggacag tacataatgc aaactgagga
     5461 atcgccggaa gaccacccgt tggtgtttac aatcggtcat tcaatgcgtc cgctcgacga
     5521 attcattggc atgctcaaaa aaaacggagt cgaacgcgtt cttgatgtac gcacagtacc
     5581 gcgctcacgc aggaatccac agttcgacaa gtccgtcctg ccggaatcat tgcccgaagc
     5641 aggcatcaag tatacgcatc tgccggagct gggcgggctg cggcacacaa agcgcgaatc
     5701 gatcaacaaa ggctggcgca atgagtcttt ccgggggtac gcggactata tgcaaacccc
     5761 ggagttcgag gcgggtgtgc gggaggtgct ggggcttgca caaaacgagc ggtgtgcgtt
     5821 gatgtgcgca gaggccgtcc cctggcgctg tcatcgttcg cttattgccg acgcccttct
     5881 ccttaggggg gctagggttg aacatatcat tcattcgaac gagaggaaat tgcacgtgat
     5941 gacaccttgg gcgcaaacgg atggattaaa aatattttat cctccatcgg aggacacgga
     6001 agattcagat aacaaatagc ataaaatacg tcgagtttcg gccggagcgg agtttcattt
     6061 gaagtcaggc cgctaaaggc gcggataggt ttgctcgaca ttgggagagc acatgaaaat
     6121 ttttttgctt attattgcaa gtctcgcgag cctcacggga ctggcttttc agcctgtgct
     6181 ggcttatcag ccagccttgc cggggcaggc aggcgccggg aaaaacccgg cctcgcagcg
     6241 gacagggcag gccggacagc ccggatcgcc ggagctcaac aatcccctgt ctcgtcctca
     6301 gaatatgcag tcgaagccat gggaggcgcg gccctgggag caggcacagg aggagcttcg
     6361 cggcgaagag ctcaagcgaa gcgatccgcg aaaaatggaa gaagggcgtc gggtcggcag
     6421 aaaggcaagg aaggagcgtg ctgcacgcct taaagaaaaa ggccaggctg tcagctacga
     6481 agatcgcgtc aaacaacgcc agacgagatg gcagcgccat cagttgcaaa gcgaccaata
     6541 ttcgactcgc atgtctcagt agagcatcgg ctctctcgtt caaacttgga tggatcacgt
     6601 tcggtgagcg gcggcaggca ggaaatcttg aatttgaaat cttccgtaat ctgattggga
     6661 atcaggtcga atagtgtccg aaacatacct cgttatcgca tcttctgctg tccttctggt
     6721 tacgatggcg ttcctggtaa tccggctggg catactttcc gcaatggtac gggatcttct
     6781 cgggcagcaa gccaggctga tggaggaaaa gcatcgcgac atgctcaagg acatgcacga
     6841 gagcttggca aaccagggag accgattatc cgacgccctt ggtaaaaatt ccgagcaact
     6901 gcggggaacg cttgaagctc gcctggacgt cattagcgga aaggtggccg agcgggtcga
     6961 ggagggtttc aggaaaacca atgagacttt cacgaatgtg atggcgcggc tggcgacgat
     7021 cgatgaggcc cagaaaaaaa tcgacagcct cacgacaaac atggtgagct tgcaggagct
     7081 tttgggagac aagcgttcaa ggggggcatt tggcgaagtt cagctcgagg cgttggtgcg
     7141 caatatcctg ccccctgccg cgtacacgat gcaatacact ctctccaatg gccatcgggt
     7201 cgattgcgtg ttgcgattac ctcctcccac cggaatggtc gctgtggact cgaagttccc
     7261 gctggaaaat tttcaccgca tgttcgatca ggccgcaagc gaggttgacc ggtcggccgc
     7321 gcagaaacaa ttcaaggcag acgtaaaaaa gcacatcgat gacattgccg gaaaatatgt
     7381 tgttcccccg gaaacctgcg atggcgcggt gatgtttgtt cctgcagagg caatctttgc
     7441 ggaaattcat gcctaccatt acgacctggt cgactacgcg atgcagaaac aagtctggat
     7501 cgtttcgccc acaaccctga tggcggtatt gaatacggcg cgggcggtgc tgaaggacct
     7561 ggaaacgcgc gagcaggtgc acatcatcaa gaacgaactt tcaaggcttg gcaaggattt
     7621 cgtccgcttc gacgaacgga tgaaaaaact ggcagatcat atcaggcagg ccaatcagga
     7681 cgtggaagag gtgcatattt ccagtcgcaa gatcagccag cgctttgccc ggatcgatgc
     7741 ggttgaactg gatgcggcgc aggctgatag tcccgccctg actccagggg gagattgacg
     7801 gggattccac gtga
//
LOCUS       DASSHJ010000120         5894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_997135, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000120 DASSHJ010000000
VERSION     DASSHJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997135"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFI43_04880"
     CDS             complement(<1..421)
                     /locus_tag="VFI43_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061495.1"
                     /translation="MTRNANLYDICDQPILEGECLTPPGRNLRFVYKKVACHPVLKYL
                     ILKGCRHPAIGLTETSPYQEMMRKAMRASADNWEDPQWIETTFVSLARLLDSVENPRW
                     QQREKAERIDSDLSRTDLQKMIDHCLEDILRVWDKDKN"
     gene            794..2971
                     /locus_tag="VFI43_04885"
     CDS             794..2971
                     /locus_tag="VFI43_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HET7061496.1"
                     /translation="MKLFAGAWMERHKLLIPVLILCAALLPGCASFGDNDPDRGATTV
                     KQDFFGDRFTTVEYLPQNWAAADSLWFYSVTQGSNMMPYDFFMVLEKKGSGELFRSNE
                     NMNFYRYLPQKATPSNPDALPVGFVKDPYKGKDYIGLTCAACHTAQINYKGKGIRVDG
                     GPAIADMESFMRDIVKTLLETRNNDEVRKRFVQNVLARGSYRTEADVIADLDTYVNRL
                     SSYITINYSTTHYGYGRLDAFGRIYNRVLEHIINTRELNELLRDPRVMGERALTEAEL
                     ESLTGKSGTVLTGTERDLIVRGIITRLNETNGWEALGKFRKKLFNPPNAPVSYPFLWD
                     TPRHDYVQWNGLTENSGLKAIARNAGEAIGVFGTLDWTEREGFSLPSFITGQGLSGKH
                     ISFDSSVNVMNLRRIEARLWKLQSPQWPENILGPIDTTRWANGQSLYNKHCASCHPRI
                     VRDEPDRRVVARMSKASNAKTDIQMAHNAVTYQGFSGILRNQYVNFGPGDTLLDRKAP
                     VAALLTKATVGVVATPEPDKWFLQRWGEWAYNLATGFRDNAIKASMKQGDYDPDTTAN
                     PVASLSAYKARPLNGIWATAPYLHNGSVPTLYDLLLPKKRLGDPEDGEYRPDEFEVGS
                     REFDPEKVGFKSSGYGGFIFNAQGVKEDTGGYTRGNSNAGHEYGARRVADESDGRVDQ
                     KTKESCENEEIKDADLDPSVKQKCLYPMSRQDRLDLLEYLKTL"
     gene            3039..4313
                     /locus_tag="VFI43_04890"
     CDS             3039..4313
                     /locus_tag="VFI43_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7061497.1"
                     /translation="MTASYLEQYDAASEADKPYLARRWMDTEPLPFFKELREKRPILV
                     TPKFTLLTRFDDCREVLNMPRVFTVEPYVPKMGDYLMMHDDDALHTREKSLMQCMLNR
                     DDLPAVREMVGKVSKEILDEAKGNIEIVNSYCRMVPATLVREYFGLTGAKREDLIEWS
                     YWNQYDTFHNQPFDLLPPEKSQSIIDRHNETSKKLGKFIVELIARRTLQVKAEEITLS
                     TLVRLDDDIVTRMLRTDYPKQLEFDIKRLGINAGGLLIGAVETTSQAVAQVIQYLMQH
                     PEWLQKAEAAATKEPVSEFDGIVWEALRFVPITPYLFRTTASDYTAAKGTGHETVLRK
                     GTYVLPVTLSAMFDDRAFESPDEFIPGRNWYNYCHFGFGSHECLGRYVAGVMIPEMVR
                     QVLMRQNVTAKAPIDYKSGPFPEQYDLSWTVQ"
     gene            complement(4341..5012)
                     /locus_tag="VFI43_04895"
     CDS             complement(4341..5012)
                     /locus_tag="VFI43_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7061498.1"
                     /translation="MQRHPLIDARRYAFVIFLLAAIYAWTSAWAGEHESGLISAAGSG
                     DLARVRAMLKSGTNVNAKAGDGVTALIAASDSGYEDVVQLLLSAKADINARTSNGVSA
                     LMRAAQRGHAQVVKELLAAGADLNARKNDGATVLMQASQEGRGDVIRTLLAAGADVNA
                     RLSNGATALMLAAQVGQTGAIRALLTGKADVNAKAENGATALMIASKYRHQEAANVLK
                     EAGAK"
     gene            5230..5757
                     /locus_tag="VFI43_04900"
     CDS             5230..5757
                     /locus_tag="VFI43_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HET7061499.1"
                     /translation="MRKTLIPSLLLLLLPLTSSAEITARPGLWEITTSSALLGMVPEV
                     PPQQLQKLTKLAKQYGVVMPKIQNGAATSRICVTPEMAGHNIPDYFHQRQSGCNVSKT
                     AETENSYKADVVCSGAQIKGNGKAEGTFVDSETFSGYTEFNGTVAGTPIKDRADTTGR
                     WIGEHCKTAQPADGL"
BASE COUNT         1481 a         1550 c         1550 g         1313 t
ORIGIN      
        1 cattcttatc tttatcccaa actcgcaaga tgtcctccag acagtgatcg atcatcttct
       61 gcaaatctgt tcgagacaga tcgctgtcga tccgctcagc cttttcgcgt tgctgccacc
      121 ggggattttc gacgctatca agaagtcgag ccaaggaaac gaaggtggtt tcgatccatt
      181 gtggatcttc ccagttatca gcgctggcgc gcatggcttt acgcatcatc tcttgataag
      241 gggatgtttc tgtcaaccct atggcagggt ggcggcaccc cttgagaatc aaatacttca
      301 gaacaggatg acaggccact ttcttgtata caaaccgaag attgcggccg ggtggggtaa
      361 ggcactctcc ttcaaggatc ggctgatcgc agatatcata gaggttcgcg tttcttgtca
      421 ttgcttttgt tcctgtataa aaactgtatc taaagacgca gcttcaaata agtgaacgac
      481 agcagaacgt ttgccggtac ctcgtattta atggcgactc gaatagaaaa aatgaggtgc
      541 tcctgcatgt ttgcggcagg tctatcgccc aggagatcta tccttccttg ctatcacgaa
      601 acattgagac ttccgtttct ggaacgagtt ttgtttcttt gaatggtaaa agcgttgccc
      661 tgaattttgc ttaagggcgg tagatcgaat tcccgcacat gcatcgctga tatttgatgg
      721 tatcgactat aataatttta aatgatcagc tgagcgacgg cctcatctca ccattagatc
      781 aagggagata gctttgaaat tatttgctgg tgcctggatg gaacgacaca agcttctcat
      841 tcctgtttta atcctctgcg ctgccctgct gcctggctgc gcatcgtttg gagacaacga
      901 cccggaccgc ggcgcgacca cggtcaagca ggactttttc ggtgatcgtt tcaccaccgt
      961 agagtatcta ccccagaact gggccgctgc tgacagcctc tggttttatt cagtgacgca
     1021 agggtcgaac atgatgcctt acgatttctt catggtcctg gagaaaaaag gaagcgggga
     1081 gttgttccgg tcaaacgaaa acatgaattt ctatcgctat cttccccaga aagcgacccc
     1141 cagcaacccg gacgcattac ccgtcggctt tgtcaaggac ccatacaaag gcaaggatta
     1201 catcggcttg acgtgcgccg cgtgccatac ggcccagata aattataagg gcaaaggcat
     1261 acgcgtcgat ggcggcccag cgattgcaga tatggagagc ttcatgcggg acatcgtcaa
     1321 aacactgctt gaaactcgaa ataatgatga ggtcaggaag cgcttcgttc agaatgtatt
     1381 ggcaagagga tcgtacagaa cagaagccga cgtgatcgcc gatctcgata catacgtcaa
     1441 ccgcttgtca agctacataa ccatcaacta cagtacgacg cactacggct atgggcggct
     1501 ggatgcattt ggacgcatct acaacagggt actggaacac atcatcaata cgagagagct
     1561 gaacgaactc ctgcgtgatc caagggtgat gggggagcgg gcccttactg aggcggaact
     1621 ggaatctctt actggcaagt caggtactgt tctgacaggc accgagcgcg acctgattgt
     1681 gcgcggcatt attacgcgct tgaatgaaac gaatggatgg gaagcactcg gaaagtttcg
     1741 aaaaaaattg ttcaatcccc ccaatgcccc cgtgagttac ccattcttgt gggacacccc
     1801 ccgccatgac tacgtgcagt ggaatggact tacggagaat tccggattga aggcaatcgc
     1861 gcggaatgcg ggcgaagcga ttggcgtgtt cggcacgctc gactggacag aacgtgaggg
     1921 attttcgcta ccgtcgttca ttacagggca aggtctctcg ggcaagcata taagctttga
     1981 ttcttctgtg aacgtcatga acctgcgccg catcgaagcg cgtctctgga aactgcaatc
     2041 cccccaatgg cccgaaaaca tccttggtcc catcgacaca acccgctggg cgaatggcca
     2101 atcgctctat aacaagcatt gcgcgagttg ccatcctcgc atcgttcggg acgaacccga
     2161 ccgtcgcgtg gtcgcacgca tgtcgaaagc gagcaacgcc aagaccgaca tccagatggc
     2221 gcataacgcg gtaacctacc agggcttctc ggggattttg cgtaatcagt acgtaaactt
     2281 cgggccaggt gatacgttgc tcgaccgtaa agcgcctgtc gccgcgctgc tgaccaaagc
     2341 aacagtcggg gtggttgcaa cacccgagcc ggataaatgg ttcctgcagc gctggggcga
     2401 atgggcgtat aacttggcaa caggatttcg ggataatgcg atcaaagcgt caatgaagca
     2461 gggagactac gatccggaca ccaccgccaa tcccgtagct tcgttgagcg cttacaaggc
     2521 gcgtccatta aacggcatct gggcaacagc tccttacctt cataatggct cggtgccgac
     2581 actctatgat ttattgctcc ccaaaaaaag gttgggtgat ccggaagacg gcgaataccg
     2641 gcctgatgag tttgaagtag ggtcacggga atttgatccg gaaaaagtgg ggttcaaaag
     2701 cagcggctat ggaggtttta tattcaatgc ccaaggtgtc aaggaggata cgggaggata
     2761 caccagggga aacagcaatg ccggacatga gtacggagcg aggcgagtgg ccgatgaatc
     2821 cgatgggcgg gtagatcaga aaaccaagga atcatgcgaa aacgaagaaa tcaaggacgc
     2881 cgacctcgac ccgtctgtca agcagaagtg cctgtacccc atgtccaggc aggatcggct
     2941 ggatttgctt gaatacctga agacactgta accagagctg gggctcgcct ctgatcttca
     3001 ggaacacaca caacttcaaa aaggataaag gagcaaagat gaccgcaagc tacctggaac
     3061 agtatgatgc ggcgtccgag gcagataagc cctatctcgc ccggcgctgg atggacaccg
     3121 aacctctgcc atttttcaag gaattgcgcg agaagcgacc catcctggta acgccgaaat
     3181 tcactctgct tacccgcttc gacgattgca gggaagtgct caacatgccc agggtattta
     3241 cggtagagcc ctacgtacca aaaatgggcg actacctgat gatgcacgac gatgatgcgc
     3301 ttcacactcg ggaaaaatcg ttgatgcagt gcatgttgaa ccgggatgac ctccctgcgg
     3361 ttcgtgaaat ggtcggcaaa gtcagcaagg aaatactgga cgaggccaag ggaaatatcg
     3421 aaattgtcaa cagctactgc cggatggtcc ctgcgaccct cgtacgggaa tatttcggat
     3481 taactggtgc aaaacgcgag gatttgatcg agtggtccta ctggaaccag tacgacacct
     3541 tccacaacca gcctttcgat cttctccccc ccgaaaaatc ccagtccatt atcgatcggc
     3601 ataatgagac gtccaaaaag ctgggaaagt ttatagtcga gctgattgcc cgccgtacgc
     3661 tacaggtgaa agctgaagaa ataacgctat cgacgcttgt acgcctggat gatgacattg
     3721 tgacgagaat gctgcgtacg gactacccaa agcaactcga gtttgatatc aagcggttgg
     3781 gaatcaatgc aggggggttg ctgataggag ccgtcgaaac aacctctcag gcagttgccc
     3841 aggttatcca gtacctgatg cagcatccgg aatggctgca gaaggcagaa gccgccgcaa
     3901 cgaaggaacc tgtgtcagag ttcgatggta tcgtttggga agctctgcga ttcgtgccga
     3961 tcacgcccta tcttttccgt accacggcaa gcgattacac ggcagccaaa ggaacgggac
     4021 atgaaacggt acttagaaaa ggcacctacg tgctgccggt aacgctttcc gcaatgtttg
     4081 acgaccgcgc attcgagtcg ccggatgagt ttattccagg ccgcaactgg tataactatt
     4141 gtcactttgg attcgggagt cacgagtgcc tggggcgcta cgttgccggc gtaatgattc
     4201 ccgaaatggt acgtcaggtc ctgatgcggc agaatgtcac ggctaaagcg ccgatcgact
     4261 acaagtccgg cccctttccg gagcaatatg acctttcgtg gacggtacag taagactttc
     4321 ttaggcctca gattacctga ttattttgcc ccggcttcct tcagcacgtt cgcggcctcc
     4381 tgatgacggt atttcgatgc gatcatcaac gcggtagccc cattttcggc tttggcattc
     4441 acatccgcct tgccagtgag aagagcacgt atcgcaccgg tttgcccgac ctgagcggca
     4501 agcatcagtg cggtcgcccc attgctcagg cgggcgttca cgtcggcgcc ggcagcaagt
     4561 aacgtccgta ttacatcgcc gcggccctcc tgcgaggcct gcatgaggac agtggcgcca
     4621 tcatttttcc gggcgttcag atcagctccc gccgcaagca attcttttac gacctgagcg
     4681 tgacctcttt gggctgcgcg catcaacgcg ctgacgccat tgctggtcct cgcattgatg
     4741 tccgctttgg ccgacagcaa tagctgaacc acatcttcgt atccgctgtc cgatgctgcg
     4801 atgagtgcag taacgccatc ccctgctttt gcattcacat tggttcccga cttaagcatt
     4861 gcccttactc gtgcaagatc gccgcttccc gctgcactga tgagcccgct ttcatgctcg
     4921 cccgcccacg cactggtcca ggcataaatt gcggccagca aaaatataac gaatgcatac
     4981 ctacgtgcgt cgatcaaggg gtgtctttgc attgaatgac tcgaattcgc ggtggtggaa
     5041 aatctacaga aactcaggct aaaaacatgc gagcacgtga aacaggtgca agctccgggt
     5101 ctgcactctc ttgttggcaa cagagtccat aacagtgcaa tatactacag ggctcgacgc
     5161 tgcccttcca ataaacacgg ctcggctaca tatcacctga ccgcgctgtc tttttaatcc
     5221 aggaaaccca tgcgaaaaac gctcatccca tcgttattgt tattactatt gccattgacc
     5281 agctcggccg aaattaccgc gcgccccggc ctgtgggaaa tcacgacgtc ctcggccttg
     5341 ttagggatgg tccccgaagt tcccccgcag caactgcaaa agttgactaa attggcgaag
     5401 cagtatggag tggtcatgcc gaaaatccag aatggcgcag caacttccag aatttgtgtc
     5461 acgccggaaa tggcgggaca taacattccg gattattttc accagcggca atccgggtgc
     5521 aacgtcagca aaaccgccga aacagaaaac agctataaag cggacgtcgt ttgttcggga
     5581 gcgcagatca agggaaacgg aaaggcagaa ggcactttcg tcgattcgga gaccttctcc
     5641 ggatataccg agtttaacgg taccgttgcc gggactccca tcaaggatcg ggcagatacc
     5701 actggccgat ggattggtga gcattgcaaa acagcccagc ctgccgacgg cctttgaaca
     5761 ggtaaaggct ggaaagggaa acaagattcg agccaggtag ccgatttacg atcaggcaat
     5821 tggcatccgc tacttaccca gcgcttccag cagtttctcg tggattccgc caaacgcgcc
     5881 attactcatg atga
//
LOCUS       DASSHJ010000121         5016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_737154, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000121 DASSHJ010000000
VERSION     DASSHJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737154"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1147
                     /locus_tag="VFI43_04905"
     CDS             245..1147
                     /locus_tag="VFI43_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7061500.1"
                     /translation="MRRKILIVLSALLCGMALAVIGVGELLSGPVPTAVSTLLTDVAV
                     EPVQISRTQWAQSGGVKNDTENEKRPIKGWLLRGVRGRGVILLVHSLRSNRVEMLSRA
                     RFLNEQGYGALLIDLQAHGETYGDEITFGVRESQDIEAAVDYLRNAFPGERRGAIGVS
                     LGAAAIVLAKNPLGLDAVVLESLHPTLREAVENRLKLHLGEFGPILTPLLLWQISPRF
                     GVSPEELDPIRHIGDLNTPVLLISGTDDRHTTVPETERLFDAARQPKELWIVPGGGHF
                     NMHSYAGKEYENRISDFFHSHLQR"
     gene            1432..2049
                     /locus_tag="VFI43_04910"
     CDS             1432..2049
                     /locus_tag="VFI43_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7061501.1"
                     /translation="MNKFSKAVIVALLFGGSISTASAGGVITGVTATGLSGDSFNVTL
                     NGGDHTLDISKILDSINPVTLTFTVSHQDGTGGSPYAVTESVTNHTGQTWTDFHYSIT
                     EPDQGNGVVFTSFNQSTFGSFTLDSAPSSGPRDLNFTGTLTDGGTAQASFSISPFDPG
                     QGNVSTFSVTQTPSVSAIPEPETYAMMMAGLLLMGSIVKRRSNKA"
     gene            2835..4337
                     /locus_tag="VFI43_04915"
     CDS             2835..4337
                     /locus_tag="VFI43_04915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7061502.1"
                     /translation="MSSISVWRATAPATPSYPRLEEDVTADVVIVGGGITGVTLALDL
                     AEQGTSVVLLEAYDLGFGSTGNSTGNLYETISRGIGKIVNRWGEDTAHAVAVARRSAV
                     DQIERRVDQYHIACEFRRCPLRRYATSDGAREHVEKEYQASLKAGLAVRLEDELPHPF
                     PPPVGPVLVLDNQAQFNPEAYVRELGRQAADRGCRLFEYSPVVEVDAEQRIVRTALAK
                     VKAKEIVLATHTPKGFHVVQAEMTVNREYGIASRVIKASYPQGIFWSSGVDRLSVRTL
                     DTKDATFLICVGEEYKTGQQDARGNLDRLETSAHAYLNTGEIEFHWSAQNYSPADGLP
                     YIGRDSSGCFIATGFETDGLTYGTLAASIIADQIAGRDNAFALLVKASRFQPVRGASG
                     IVEENVSVIKSFIKDYVTDRKTEPLSHLEPGEGALVEVNNESVAAYKDPEGKVIAVSP
                     VCTHMKCKVHWNDVEKTWDCPCHGSRFAPDGSVIEGPAIQPLERKQIAEH"
     gene            complement(4352..>5016)
                     /locus_tag="VFI43_04920"
     CDS             complement(4352..>5016)
                     /locus_tag="VFI43_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061503.1"
                     /translation="VLALGGCATPTTSFLGGTYPRLSYYDLRKREVPLRLKLSVEFQR
                     NGQHFPKGDVPLRDYANQILRDSGVITPVEEGEEGEIRVVLNNVADPQTVAAERESTM
                     HPLWMVGTTITDAYELSMFITMKGKTIARMGIKNAVHTALGNIRIPDTIQTFPHNQAF
                     GKLLEQMMLRALVDMQRTRELATLASIAPPAMEHGLRQGAVRTFRSFSRKPTQPLPPS
                     GL"
BASE COUNT         1155 a         1405 c         1352 g         1104 t
ORIGIN      
        1 ggacacagca tttgcgaaga tacgggcgcg tgtgcaagga actaggcttg cattcaggac
       61 tctggcgggg accgtgcgag actgtgtttg aagctgttcc aggtaaataa tcagcggaag
      121 gtcggtttcc aggcaaagaa ataagggctt tgttccccgc tcgctgcacg caagtcgccg
      181 atcgccttcg gcaaaatgat cgcgttccgg gaactcctta cctatccgca gaccggatca
      241 aaaaatgcgg cgcaaaatcc tgatcgtttt atctgccctc ctctgcggca tggcgcttgc
      301 cgtaatcggg gttggcgagc ttctctccgg acctgttccc acagctgtaa gtaccctgct
      361 gaccgatgtt gcagtcgagc ccgtccagat ttcgcggacg caatgggcgc aaagcggtgg
      421 ggtgaaaaat gacactgaaa atgaaaagag gcccatcaaa gggtggctgt tgcggggggt
      481 tcgaggccgc ggcgtgatac tactggtcca ttcgcttcgg agcaaccggg tggaaatgct
      541 gagccgggcg cgtttcctga acgagcaagg ctatggagca cttctgatag acctccaggc
      601 gcatggggag acatacggag acgaaatcac cttcggcgtg cgggagtcgc aagacatcga
      661 agcggctgtc gattacctgc gaaacgcctt tccgggagaa aggcggggtg cgatcggagt
      721 ctcgctggga gctgccgcta ttgtgctcgc caagaatccg ctcgggctgg acgcagttgt
      781 cctcgaatcc ctccatccca ccctccggga agcagtcgaa aaccgcctga aacttcatct
      841 cggcgagttc gggccgatac tcacgccgct gctgctgtgg cagatatcgc cccgcttcgg
      901 tgtctctcca gaggaactgg atcccatccg gcacattggc gacctcaata ccccggtatt
      961 gctcatttcg gggaccgatg accgccacac caccgtacct gaaactgaac gcctctttga
     1021 tgcggcccgg cagccgaagg aactatggat cgttcccggc ggcgggcatt tcaacatgca
     1081 cagctatgca ggcaaggaat atgaaaaccg gatatcagat tttttccact ctcacctgca
     1141 aagataaaat cattcgcacc ctcttccggg aaagccacca gactgggata cggtaaaatt
     1201 ctgcggggtt ttcacctgct gttcccgcag tgaatccatc tactttccct tgttttccag
     1261 aaagaagcgg atggtaataa cggcctatat catctgtaga caatatatga tagtttaccg
     1321 atgaccctcg ccaagcccgt gtttttccga ggtttacggg ctgtgatggt tttatctgca
     1381 ctatctgttg ttctgatgct tcagctaata aataaagata aggagaaaaa aatgaataaa
     1441 ttttcaaaag ctgtcatcgt cgctttactt ttcggaggtt ccatctcgac cgcgagcgcc
     1501 ggcggcgtta tcaccggagt tacggcaacc ggcttgagtg gagactcgtt caacgttact
     1561 cttaatggtg gtgatcacac cctcgacatc tccaaaatcc tggacagcat caatcccgtg
     1621 acgctgacct ttaccgtctc ccaccaggat ggtactggag gttcccccta cgccgtgacc
     1681 gagagcgtta ccaatcatac ggggcaaacg tggaccgatt tccactacag tattacggaa
     1741 cccgatcaag gcaatggcgt ggtgtttaca tcattcaacc aatcgacctt tgggagcttt
     1801 acgctcgatt cggctccttc gagcggtcct cgcgatctta acttcacggg aacgcttaca
     1861 gacggcggga cggcccaggc atcgttttcg ataagcccgt tcgatccggg tcaaggtaac
     1921 gtctcaacct ttagcgtaac ccagaccccg tctgtttccg caatcccgga accggaaacc
     1981 tatgcaatga tgatggccgg tctgttgttg atgggcagca tcgtaaagcg cagaagcaac
     2041 aaggcataac tgattccctg aaactggtat cgggaaaaac cccgctctgc ggggtttttt
     2101 ttagccttaa tccgccgttg atgtccattg tccccacgct gctactctcg tctttcggga
     2161 tttatctccc tgtttacaca tcgcccatct agcagcataa atgggcctgt ctccagtttg
     2221 agacaaattt tattcttttt ttcattagct tacgcgtaat acatgaaaag gcgtcctcaa
     2281 ataaagacac ggcaatacgc ccatttttcc ggagcccggc cccgaccagg gcttgcgtgt
     2341 tgctttccaa cgacagatat ccgtcggctg actcttgcaa ccctagccca agtttctgat
     2401 ttgaatcgag ttataaatcc tggcacgata aatgcttcct tgactattgg cgctcggcag
     2461 gaggcaaaat ctccgtatat aaaacaagtc ttgcagaaaa gctccacgcc aacgctgaag
     2521 ctcatttcaa ggagataaaa aatgaccaag acattgaaca acaagatccg gggttgaact
     2581 ggcttaacct ggacaagtag cagaaatgcc ccgcctatgg cggggttttt tgtttccgtc
     2641 tccgggtcat gccaaatcaa tttgcgcacc cccacacaca gggcgagctg tgtgccgtag
     2701 cgtacagaat tagccggaag cacgctcgac aatatggcag cgttgccgta ttgccactgc
     2761 attcaccgtt tccgccagcg gcgccagcaa tcatcaactg tcgaaaacat ggacgttctt
     2821 aaaaaggata aatcatgagc tcaatttcgg tctggcgcgc tacagcgccc gctacaccct
     2881 cttatccgag gctcgaggaa gacgttaccg ccgatgtggt aattgtagga ggaggcatta
     2941 ctggcgtcac cttggccctg gaccttgccg agcaagggac ctcggtcgtg ctgctcgagg
     3001 cctatgatct tggcttcggc agcacgggca attcgacagg aaatctctac gaaaccatca
     3061 gtcgcggtat tggcaagatc gtgaaccggt ggggggagga cacagctcat gcggttgccg
     3121 ttgccaggcg gtcagctgtt gaccagatcg agcggcgagt tgatcaatat cacatcgcgt
     3181 gcgaattccg gcgatgccca ctccgccgct acgcgacctc tgacggcgcc cgggagcatg
     3241 tggaaaagga atatcaagcc tcgctcaagg cgggactggc ggtgcggctg gaggatgagt
     3301 tgccccaccc ctttcccccg cccgtcggtc cagtgctcgt actggacaac caggcgcaat
     3361 tcaatcccga ggcttatgtt cgggagctcg gccgccaggc agcagaccgg ggttgccgcc
     3421 tttttgaata ctctccggtt gtggaggtcg acgccgaaca gcgtatcgtg cgtaccgcct
     3481 tggccaaggt gaaggccaaa gaaatcgtac tcgcgacaca caccccaaag ggatttcatg
     3541 tcgtccaggc ggaaatgaca gtcaaccggg agtacggcat cgccagccgg gttatcaagg
     3601 cctcttaccc tcaaggaatt ttctggagca gcggcgtgga tcgcctctcg gtacgtaccc
     3661 tggatacgaa ggacgctacc ttcctgatct gtgtgggcga agagtacaag accgggcagc
     3721 aagatgcacg gggcaatctg gacagactgg agacttcggc ccacgcatat ctgaataccg
     3781 gcgagatcga atttcactgg tccgcacaga actacagccc ggccgacggt ctgccctaca
     3841 tcgggcgcga ctcgtccggg tgcttcattg caaccggttt tgagaccgat ggccttacct
     3901 acggaacgct tgccgcatcg atcattgccg accagatcgc ggggcgggac aacgcattcg
     3961 cgctactcgt caaggcaagc cggtttcagc cggtaagagg ggcaagcggc atcgtggaag
     4021 aaaacgtttc cgtaataaaa tccttcatca aggactacgt gacagaccgc aagaccgagc
     4081 cgctttcgca tctggagcca ggcgaaggag ccctcgtcga agtcaataac gagtccgttg
     4141 ctgcatacaa ggatccggag ggcaaagtaa ttgccgtgtc gcccgtttgt acccatatga
     4201 agtgcaaggt ccactggaac gacgtggaaa aaacctggga ttgcccctgt catggaagcc
     4261 gctttgcacc ggacggaagc gtgatcgagg ggccggcaat ccaaccgctg gagcgcaagc
     4321 agatcgcaga gcactaaaaa ggaggcttcg ttcataagcc gctcggcggc aaaggctggg
     4381 tcggcttccg cgaaaaagat cgaaaagtcc taaccgcccc ctgtcgtaac ccatgctcca
     4441 tcgccggcgg tgcaatagaa gccagggtgg cgagttcacg ggtcctttgc atgtcaacca
     4501 gggcgcgaag catcatctgc tccagtaact ttccgaaagc ctggttgtgg ggaaaagtct
     4561 ggatagtgtc cggtatacgt atatttccga gcgcggtgtg aactgcgttt ttgatgccca
     4621 tgcgcgcgat cgtttttcct ttcatggtaa tgaacatcga aagctcatag gcgtcggtaa
     4681 tcgtcgtacc gaccatccac aatggatgca tcgtgctctc ccgttcggct gcaacagtct
     4741 gtgggtccgc cacattgttc aggaccaccc tgatctcgcc ctcttccccc tcctccactg
     4801 gcgttattac ccccgagtcc cttagaatct gattggcata gtccctcagg ggaacgtccc
     4861 ctttcggaaa atgctggcca ttgcgctgga actccacgct cagcttgaga cgcagaggaa
     4921 cctcgcgctt tctgaggtcg taatatgaca atctgggata agtgccgccg agaaacgacg
     4981 ttgtcggcgt tgcgcacccc cccagcgcca gaacga
//
LOCUS       DASSHJ010000122         9257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_778673, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000122 DASSHJ010000000
VERSION     DASSHJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778673"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..3470
                     /locus_tag="VFI43_04925"
     CDS             129..3470
                     /locus_tag="VFI43_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061504.1"
                     /translation="MSSDDNKGSWFQNPIPIVMVTLMAAGVLVKVVPLESARPGEPDR
                     TKSVLMVEQDVEARLWQDPLSAVENEISAQSRGDKKQSETASGHIHKPDALLAKIKQT
                     ITDKKEKNKVTILAVNLFGGSFDASAEWRRRTRFAVLSALEFQGYSPENADALGYYVT
                     TLQNDYDDKGFRNVELDIPYEWFSRSEKEEDERSRVLVLWLNEDKLLRPYKNLLSLLG
                     ELSTGLNLNTKVVGPAGSGTLLKLIKEAHDDAEVKQNRNLKGGGTLDIFSPTATISNC
                     DLYTMKAVAERHVMQWNCFMSSAEMNMDALSDLPIVRTIATDDVLSAAVLWELWHRGI
                     NRSRPWPAKVDGHSADESVVPSAKCDDGLVLLREWDTQYAESLARNIQDGFSGLCGAK
                     AAPLKTFSYLRGLDGAVPAGEATEANIQAKSSRQSSDESKDLRKQLEDAPPEHAEGRS
                     QYDYLRRLTAEIERLDKNRQFAGNGIKAIGVLGSDVYDKLLILMALRARFPNKIFFTT
                     ELDARYLHADQKDWSRNLVVISSFGLTLEPALQKSALPFRDTYQTGIYLAALAALEEE
                     PAHGNSYTVFWADKMKKWLRPGIFEIGRDQAVHLASPSIDDLADWIKGVNMQGIQQIR
                     PNKPQCPEENWTKCDQIQPTRQGDVGTSQGGIILAVIVAGALLAILANRHLQQLFTLA
                     FNTQAPGHSTAKSALIGAAAGLLITALIMELIRRSLNQSLDHGVGEPFEWFEGVSVWP
                     SLTISFASLILMTAIICLLKFKLERDAAFISRYFGLDLPTSYSLGRNRISALFVGPHI
                     DVSGLDKDGKRIADELLRSGAKLQVSTLWQNYLRVTNWREALEWVTLSTVTALIFVAL
                     LFFLYGNPVFPHRGDLVQNLNAILGFLNALMLWAVIFWTSYEARACARLINTLGKTGE
                     HLWSDPPIETLDARLGVRTDDLTPYIDFRLITALTRRIQLLIYLPFISILFIIVGRSD
                     LFDAMDFPPALIAVVVLSLGYAIYTEIVLRSAALDARGQILKEYEERLFVMEGNVPAE
                     APDRTRDAPLATATPGNSSQTSTSRPPITAEQIKLLMERIRNTHEGAYAPISQQPALQ
                     ALLLPFGGLGGVQLIEYLMSFTISH"
     gene            3555..4154
                     /locus_tag="VFI43_04930"
     CDS             3555..4154
                     /locus_tag="VFI43_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061505.1"
                     /translation="MKRLSIIAGFALGNFTCSVAVIAQNMEFSSLNAMQVLPPVDQHA
                     FISGDEIHTKDRVMDSSAGLSSRPVIVLPVPLSQGRQLPADPYRFHYPGSYIPRDTPL
                     HRFNGYIVCDPPDAAKKKGEARQGTNTGKPTDQLPEKAATPRLVKITSTRPLAAPDYL
                     KSCPIYTINGQIQRDASVMASPSADGEDSRPAQRAGSIP"
     gene            complement(4246..4860)
                     /locus_tag="VFI43_04935"
     CDS             complement(4246..4860)
                     /locus_tag="VFI43_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061506.1"
                     /translation="MNFPDFYDNAPVVRTRDPFAHMLGAAQDGMLEYRYLDAVRLAGH
                     SCPTVAGAFLMGRAALSALYPDEPAERGAIAVHMPAPEHEGTTGVMAQVLTLLTGAAA
                     DNGFHGIQGRYRRKGLLSFSEQREGEAIIFTRLDNGKSVGVSLNVSLVPGDPAQGARL
                     AAILQDHADEAQRVAFANAWQERVRRLLLEFADDPRVIRVSTIQ"
     gene            complement(4847..5329)
                     /locus_tag="VFI43_04940"
     CDS             complement(4847..5329)
                     /locus_tag="VFI43_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7061507.1"
                     /translation="MPRIGPLLALSREHHQTLVMARDARRAAVSNDEAVLSRMIARME
                     DHWHNVMADHCRREERLVKVAEDSLDPDSVARFISEHEELQRLGTGTCQLSPVIRLQR
                     FADLASAHVRYEERVFFSQLQSHPSIASSTEPDNSGHGHLERVNIDSLRDIGDVDEFP
                     "
     gene            complement(5401..5982)
                     /locus_tag="VFI43_04945"
     CDS             complement(5401..5982)
                     /locus_tag="VFI43_04945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7061508.1"
                     /translation="MMEGTELKLHTVICSTRPSRIGPRIAVWFHELAVLHGQFHASLV
                     DLADFDLPVYDEPEHPVRQNYRNEHTKRWAAAVNSADAYVFVTPEYNYSPPPALLNAL
                     DYVYKEWNYKPAGIVSYGGISGGVRAAQMEKLTLTTLKIMPVLESVVIQNVSSYFDDQ
                     ENFLPEDKHKSAGSALLNELHKWALALKTMRRP"
     gene            complement(6192..6545)
                     /locus_tag="VFI43_04950"
     CDS             complement(6192..6545)
                     /locus_tag="VFI43_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7061509.1"
                     /translation="MKTNKLVLSFLVVGALLGSAAGCSSTDSGRHRSDAGNRSEAGEY
                     VKDSVITSKIKAKLAAEKDVSAMNITVDTDNNGVVVLGGTASSQAEKDKAHSIAHSVE
                     GVTKVINHIKIKKDY"
     gene            complement(7408..8688)
                     /locus_tag="VFI43_04955"
     CDS             complement(7408..8688)
                     /locus_tag="VFI43_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7061510.1"
                     /translation="MKLESLALHYGYASDPTTRAAAVPIYQTTSYTFDNTQHGADLFD
                     LKVQGNIYTRIMNPTNAVLESRIAEMEGGVGALAVASGMAAITYAIQCIASAGDNIVS
                     TTQLYGGTYNLFAHTFPRQGIEARMVSYDDYEGIARSIDGKTRVIFCESIGNPAGNIV
                     DIERLASIAHEQGVPLIVDSTVSTPYLCQPFKIGADVVVHSLTKYIGGHGTTIGGMIV
                     DSGNFDWVKHRERFPLLNEPDPSYHGVVYTEAFGNAAFIGRCRVVPLRNTGAALSPLN
                     AFLILQGLETLGLRMERHCENALKVAQYLEQHPKVEWVNYAALPGSKHHELCMRLSQG
                     KASGILSFGIRGGIESGTQFIDALKMILRLVNIGDAKSLACHPASTTHRQLNPNELKA
                     SGVTEGLVRISVGVEHIDDIIADIDQALDAVGAH"
     gene            complement(8851..>9257)
                     /locus_tag="VFI43_04960"
     CDS             complement(8851..>9257)
                     /locus_tag="VFI43_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET7061511.1"
                     /translation="HNYGITLASDGPDLQKRRGDRKYEQSHTFSNIISTRYPWLNLVT
                     LNFGYHNAHHAKPTVPWHELPKLHEALYGSKAGRVKDPVIPFAKQLSSFHKGRVARIF
                     GDPAETNGIRFAENLDNGRAVGASGVSFLTPL"
BASE COUNT         2261 a         2452 c         2316 g         2228 t
ORIGIN      
        1 taccgtttca ttatgatttt tctcttgtat cgcagtatct tcgaattgcc acgctaggtt
       61 tctgacaata gaaagaccgt cgacttagag gaaattaaac aggtagccat ccaggcacga
      121 atcatcatat gagcagcgac gacaataaag gcagctggtt tcaaaatccg atacccatcg
      181 tgatggtgac tcttatggcg gcaggcgtgc tggtaaaggt ggtacccctc gaaagcgctc
      241 gtccgggaga acctgaccga acaaaatcgg tattgatggt cgagcaggat gtcgaggcgc
      301 gcttatggca ggatccgttg tccgccgtcg agaacgagat ttcggcccaa tccagagggg
      361 ataagaagca atcggaaact gcatccgggc acatccataa acctgacgct cttctcgcca
      421 agattaaaca aacaattact gacaagaaag aaaaaaataa ggtaaccatt cttgctgtaa
      481 atctgttcgg cggctcgttc gacgcatcgg ccgagtggcg caggcgaacg cgctttgctg
      541 tgctctccgc tctcgagttc caaggttata gccctgaaaa cgccgacgcc ctcgggtatt
      601 acgttacaac gcttcaaaat gactatgacg acaaaggctt caggaatgtt gaactcgata
      661 tcccgtacga gtggttttcc cgctcagaaa aagaagaaga cgagcgatcc cgggtactcg
      721 tactgtggtt gaatgaagac aagcttctga ggccttataa aaacctgctt tctctcctcg
      781 gcgagctaag cactggcctt aaccttaaca ccaaggttgt gggccccgcg ggctcaggga
      841 ccctactgaa gcttatcaag gaggcacacg acgatgcgga ggttaagcag aaccgtaatc
      901 ttaaaggtgg cggcacgctt gatatatttt ctccgaccgc aaccatctcc aattgtgatt
      961 tatataccat gaaggccgtt gcggaaaggc acgtcatgca atggaattgc ttcatgtcgt
     1021 ccgcggaaat gaacatggac gctctgtcag accttcccat cgttcgcacc atcgcgacgg
     1081 acgatgttct ttcagccgcc gtcttatggg aattatggca ccggggaatc aatcgctcac
     1141 gtccatggcc agctaaagtg gatgggcatt cggccgacga aagtgtcgtg ccttcagcca
     1201 agtgtgatga cggactggtc ctgctcaggg aatgggatac gcaatacgcg gagtcattgg
     1261 cgaggaatat ccaggatggt ttttccggcc tttgcggagc gaaagctgcg ccactgaaga
     1321 cattttcata tctacgtggc ctcgatggag cggtccctgc aggcgaagca acagaagcaa
     1381 atattcaggc caaaagcagc aggcaaagca gcgatgaatc gaaagacctg cgcaagcagc
     1441 tggaagatgc tcccccagag cacgccgagg gacgaagcca atatgattac ttgcgtcggc
     1501 tgacagcgga gatagaacgg ctggacaaga atagacaatt tgccggtaac gggatcaagg
     1561 cgatcggcgt tctgggatcg gatgtgtacg acaagcttct catcctcatg gcgttgaggg
     1621 ccaggtttcc caacaagatt ttttttacca ccgagcttga tgcccgttat ctacacgccg
     1681 atcagaagga ttggtcaagg aaccttgtcg tcatatcgag ctttggtttg acgctggagc
     1741 ccgcactgca aaaatcggcc ttgccattcc gggatactta ccagacggga atctatctcg
     1801 ccgcgcttgc agcgcttgag gaagaaccgg cgcatggaaa cagctacaca gtgttttggg
     1861 ccgacaagat gaagaaatgg ttacgtccgg gaattttcga gatcggcagg gatcaggcag
     1921 tgcacttagc ctcaccctca atagatgatc ttgcagactg gattaaaggg gtcaacatgc
     1981 aaggaattca gcaaatcaga cccaataaac cgcagtgtcc tgaagaaaat tggacaaaat
     2041 gcgatcaaat tcaacccacc cggcagggag atgtcggaac gagccaaggg gggatcatcc
     2101 ttgccgtcat cgtcgctgga gctttactgg ctattttggc caatcgtcat ctacaacaac
     2161 ttttcacctt ggcgttcaac acgcaagcac cgggccactc aacagcaaaa agcgcgttga
     2221 ttggagcagc agcaggccta ttgattacgg ctctgattat ggagctgatc aggagaagct
     2281 tgaatcagtc gctcgatcac ggcgttggag aaccttttga gtggtttgaa ggtgtaagcg
     2341 tctggcctag cctcacgatc agctttgcta gtctaatatt gatgacagcg atcatttgcc
     2401 tgttgaaatt caagcttgaa cgggatgctg ccttcatctc ccgctatttt gggcttgatc
     2461 tgccgaccag ctacagtctc ggccgcaaca gaatatccgc gctgtttgtt gggccgcata
     2521 ttgatgtatc gggacttgac aaagatggaa agagaatagc ggatgagctt ctgcgatcag
     2581 gggcgaagct ccaggtgtcc acactgtggc aaaactactt gcgcgtcacc aattggcgcg
     2641 aggcacttga atgggtaacg ctttcaaccg tgactgcgct tattttcgtt gcactgctat
     2701 ttttcctgta cggtaaccct gtgtttccgc accgcggaga tctggtgcag aatctgaatg
     2761 cgatccttgg atttctcaac gccctgatgt tgtgggcagt tatcttctgg acgagctacg
     2821 aagcgagggc atgcgcgaga ttgatcaata cgctgggaaa aaccggggag catctatggt
     2881 ccgatccgcc cattgaaacg cttgatgcca ggttaggagt gcgtaccgat gatttaaccc
     2941 cttatatcga ctttcggttg attactgctt tgacccgacg catacaactg ctgatatatc
     3001 tgccttttat ctcgattcta ttcataattg tcgggcgcag tgatcttttt gatgcaatgg
     3061 atttcccccc ggccttgatc gctgtcgttg tgctctcgct cggttatgcg atatataccg
     3121 aaattgtgct tcgcagcgct gcgctcgacg ctcgcggcca gatacttaag gagtatgaag
     3181 agaggctttt tgtaatggaa ggaaatgtgc ctgccgaagc tccagataga actcgcgatg
     3241 ctccgttagc aacggcaact cccggcaaca gttcgcaaac gagtacttca cgaccgccta
     3301 taactgccga gcaaattaag cttttgatgg aaagaatccg taatacacat gaaggcgcct
     3361 atgcgccgat atcgcagcaa cccgcacttc aggccctgct cttgccgttc ggggggttgg
     3421 ggggcgtgca attgattgaa tatctcatga gctttacgat atcccattga accaatagaa
     3481 atgctatgaa gaaaagtgat aacgcggttg atcacctgtg tttacttcac ggcatagata
     3541 aaagcaggaa agccgtgaaa cgactatcaa ttattgccgg gttcgcgctt gggaatttta
     3601 cctgttcggt tgccgtgatt gcccagaata tggagttctc aagcttgaac gccatgcaag
     3661 ttctaccgcc ggttgatcaa catgcattta tttccggaga tgagattcat accaaagatc
     3721 gggtaatgga tagcagcgca ggtctgtcct ccaggccggt tatcgtactc cccgttccac
     3781 tttcccaggg aagacagctt cccgccgatc cttatcggtt tcactaccct ggtagttata
     3841 tccctcgtga cacccccctg catcgtttta atggatacat cgtatgcgat cctcctgacg
     3901 ccgccaaaaa aaaaggagaa gcgcgccagg gcacaaacac aggcaaacca acggatcagc
     3961 taccggaaaa ggcggcgaca ccccggcttg tcaagattac ctcgacacgg cccctcgccg
     4021 cgcccgatta tctgaagtcc tgccccatct acacgattaa cggacaaatc cagagggacg
     4081 cgagcgtcat ggcgagccct tcggcggacg gggaagatag tcgacccgcg caacgggcgg
     4141 ggtctattcc gtgaccgtaa tctggactgg caatgcccag cctttgtctg ccatcttatg
     4201 ctctatatgg gcgacgatct gacgggcaga aaggcgataa gccgattatt gaatcgtact
     4261 aacgcgtatc accctcgggt catccgcgaa ctccaacaac aaccgccgca cccgctcttg
     4321 ccatgcattg gcaaaggcaa cccgttgcgc ctcatcggca tgatcctgca gaatcgctgc
     4381 caagcgggct ccttgcgctg gatcgccggg cacgagcgaa acattaaggg aaactcctac
     4441 actcttgcca ttgtccaggc gggtaaatat gattgcctca ccttcgcgct gttctgaaaa
     4501 gcttagcagc ccttttcgcc tgtagcgccc ttgaatccca tgaaagccgt tgtccgcagc
     4561 agctcctgta agcaatgtca ggacctgcgc catcaccccc gtggttcctt catgctcggg
     4621 cgcaggcata tgcactgcaa tagcgccacg ctccgcgggt tcatcaggat atagcgcact
     4681 caatgcagct cgtcccatca gaaacgctcc ggccaccgta ggacatgagt gtccggcaag
     4741 ccgaactgca tcaaggtagc ggtattcaag cattccatcc tgggccgcgc cgagcatatg
     4801 tgcaaatgga tcgcgagtcc gaacgaccgg ggcgttatcg taaaaatcag ggaaattcat
     4861 ccacatctcc tatgtctctc aaggaatcga tattcaccct ctccaaatgc ccatgcccag
     4921 aattatccgg ctcggtggaa gatgcaatgg atgggtgaga ctgcaactgg gaaaaaaata
     4981 cgcgctcctc atagcgtacg tgagcactgg caagatccgc gaacctttgc aagcgtatga
     5041 cgggggaaag ctggcaagta ccggtgccaa gcctttgcag ttcctcatgt tcagatataa
     5101 accgcgcgac cgaatcggga tccagcgaat cttccgccac ctttaccaag cgttcttcac
     5161 gcctgcaatg gtctgccata acgttatgcc agtgatcttc catacgggca atcatccggg
     5221 ataaaactgc ttcatcattg cttacggctg cccgccgtgc atcgcgcgcc atcaccaatg
     5281 tctgatgatg ctcgcgtgac agtgcaagca agggaccaat tcggggcata tgaaagcgat
     5341 gctcgtaatc ggggcagtac tcgaatacaa aaggtgaccc tgagtaacac ttttcgtgtg
     5401 ctacggacgc ctcattgttt tcagtgccag agcccatttg tggagttcgt tcagcaaggc
     5461 ggaacctgcg ctcttgtgct tatcttccgg cagaaagttc tcctggtcat cgaaatagct
     5521 cgatacgttc tggataacaa ccgactcgag cacgggcatg attttcagcg tcgtcaacgt
     5581 gagcttttcc atctgcgccg cgcgcacgcc gcccgatatc ccgccatagc tgacaattcc
     5641 tgcgggttta tagttccatt ccttgtacac gtagtccagc gcattcaaaa gagcgggcgg
     5701 cggactgtag ttatattccg gagtcacgaa cacgtaggcg tcagcagagt tcaccgcggc
     5761 cgcccatctc ttcgtgtgct cgttccgata gttctgacga acgggatgtt caggctcatc
     5821 ataaacagga agatcgaagt cagcaagatc gaccagtgac gcgtgaaatt gcccgtgcag
     5881 caccgcaagc tcatgaaacc agaccgcaat ccgcggtcca atcctgcttg gccgggtact
     5941 acaaataacc gtatgaagtt taagctctgt cccttccatc atcggaccac ctatagagtt
     6001 ttcgcttccc tctgcaagta tagtctgacg gggacggcag ggcgatgcca ggacaggcca
     6061 aggaaaaatt cgcttttccc aatcctgcca catacaggtt gaacacttca ccttttctgt
     6121 tcccttctgg cggaggttga aacgataaaa aaaggccggg gttcagcccg gcctcgcgtt
     6181 gctgtgaatt actagtaatc ctttttgatc ttgatatggt taataacctt cgtgacacct
     6241 tccaccgaat gggcaatgga atgagctttg tccttttcgg cttggctact cgctgttcca
     6301 cccagcacga ccacgccatt attgtccgta tcgaccgtga tgttcattgc gcttacatcc
     6361 ttttcggctg ccaatttggc ctttattttg cttgttatta ccgaatcctt cacatattcc
     6421 ccagcctcag aacggttccc agcgtccgaa cgatgtctac ctgaatccgt ggagctgcac
     6481 ccagcagcac tccccaacag ggcgccaacc accagaaagc ttagaacaag tttatttgtt
     6541 ttcatgattt ttcctttgaa attaagggag tgtgaatgat gcagcagaaa gcgggcacgg
     6601 tctgttcggt tccatacaca atggctcagc taggggcgcg tgaaaagaaa ccccaagagt
     6661 tttttcacac tgagcccatt tcttaaatat gcttgccaaa ggtttagcat ttatttataa
     6721 tgcgaatcat tatcattctt aattaacgaa tataacgaaa gcattatgag ctcctcaaaa
     6781 cattccaagt ggatttcagg ttccataagg atttgatcac atccatagac ttatccgttg
     6841 ccgccgagtt ttctccttct tggcggcttt cagccagcca gccctaccct cgggcaagcc
     6901 agctattttt ttattcagta gtacaggttg tgaggtgggg ttatgaggcg gtggcgagaa
     6961 ctactttcat gtaaccccct tcgctttttc gtccctgaag aaacttcttt cgattttttc
     7021 gccagtgcta tacgcatagc caactcttgc atccatcagt ccgccagttc ttaagtgcag
     7081 tccttgcgtg actgatggct cggattttta agataggtcg aaaaacatta gcggaggaaa
     7141 aaatgaacgg ggctatgttt gcagcaaggt ttgtgtttat cggagtgatt gcaagaccat
     7201 gaatgcggcg cccggagtaa cttcgatcaa gtcctgtacc cggggtgccg gcgagccgcg
     7261 aagagagaaa cctgcaaaaa gggaagaggc aaatgtgtct gccgatgaag agcgcaatgc
     7321 tggcttccct tgcctctcaa gcggaatacg agaaagaaat gttctgcttt ttcgagtgat
     7381 attcgagcgg tatttgtgca aatcgcatta atgcgcacct accgcgtcca gcgcttgatc
     7441 gatatcggcg ataatgtcat caatatgctc gacgcctacc gaaatcctta caagaccctc
     7501 cgtaacaccg gatgctttca actcgttcgg attcaactgc cgatgcgtcg tcgacgcggg
     7561 gtggcaggca agggattttg catcccctat attgacgagg cgcagtatca tcttcagcgc
     7621 gtcaatgaac tgggtacctg attcaattcc tccccggatt ccaaagctta atatccccga
     7681 tgcctttccc tggctgagac gcatgcagag ttcgtggtgc ttgcttcccg gcagtgcggc
     7741 gtaattgacc cactccacct tgggatgctg ttccagatat tgcgcaactt tcagtgcatt
     7801 ttcacaatgc cgctccattc tcagacccaa cgtttccaaa ccctgcaata tcagaaatgc
     7861 gttgagggga gacaaagccg cgccggtgtt gcgcagcggc acgaccctgc accgtccgat
     7921 aaaagcagca ttcccgaaag cctcggtata caccacgccg tgatatgagg gatccggttc
     7981 attcagcagg ggaaagcgct ccctgtgctt gacccagtcg aaattcccgg aatcgacgat
     8041 catgccccct atcgtggtgc cgtgcccgcc aatatatttc gtcagcgaat gcaccacaac
     8101 gtctgcgccg atcttgaatg gctggcacaa ataaggtgtt gaaacggtgc tatcaacgat
     8161 cagtggcaca ccttgctcat gcgcaatgct tgccagtctt tcgatatcaa caatatttcc
     8221 cgccggatta ccgatcgatt cacagaaaat gacacgcgtc tttccgtcga tgctacgagc
     8281 gattccctcg tagtcatcgt acgaaaccat acgcgcttcg atcccctgcc gcggaaaagt
     8341 atgtgcaaag agattgtatg tgcctccata aagctgggtt gtgcttacaa tattgtcacc
     8401 ggcactggcg atgcactgga tcgcgtacgt aatcgctgcc atgcccgatg caactgccag
     8461 tgcgccgact ccaccctcca tctcagcgat gcgcgattcc agcaccgcat tagtgggatt
     8521 catgattcgc gtataaatat tcccctgcac cttcagatcg aacaggtcag ccccatgctg
     8581 cgtgttatcg aaggtgtatg atgtcgtctg ataaattggt actgccgctg ctcgcgttgt
     8641 cggatccgat gcatatccgt aatgcaacgc caatgattcg agtttcacat gtcctcctcg
     8701 tatgtggcag cggaaaaatt aaggtgaagc ggataataga atgctcgcaa agtgtctccg
     8761 gcaaaaatgc cgctcctctc cgtcgtaaaa gagccaccgg gcattatttc gggctcgcgc
     8821 agaaatcggc cgcagacatt acagccgttg tcatagaggg gttaaaaaag acactccgct
     8881 ggctcccacc gctcggccgt tatcgagatt ttccgcaaac cggattccat tcgtttccgc
     8941 cggatcgcca aatatccgcg ctacccttcc cttatgaaag ctcgatagct gttttgcgaa
     9001 ggggataacg ggatccttca ccctgcctgc cttgctccca tacagcgctt catgcaactt
     9061 tggcagctcg tgccaaggta cagtgggctt tgcatgatgt gcattatgat agccaaagtt
     9121 gagcgtaaca agatttaacc atggatatcg ggtcgaaata atattactaa aagtatggga
     9181 ttgctcatac ttgcggtcac ccctccgctt ctgcagatcc gggccgtcag atgcgagggt
     9241 aattccatag ttgtgtt
//
LOCUS       DASSHJ010000123         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_144463, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000123 DASSHJ010000000
VERSION     DASSHJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144463"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFI43_04965"
     CDS             complement(<1..497)
                     /locus_tag="VFI43_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7061512.1"
                     /translation="MLFERIKSLDHILESAEKKGLKRQLGAFDLTMLGIGAIIGTGIF
                     VLTSVAAQKAGPGMMYSFAIAGFVCALTALVYSEIAAIVPVAGSAYTYSYAVFGEMTA
                     WMVGWALILEYAVAAAAVAVGWSGYINGFLHEIDYGLPLALTAGPYDTVVARLHDSDA
                     ATSPG"
     gene            782..1798
                     /locus_tag="VFI43_04970"
     CDS             782..1798
                     /locus_tag="VFI43_04970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7061513.1"
                     /translation="MAGVRSWAVHGAKERLEPFVYEPGPLGLEEVEIAVEYCGLCHSD
                     LSIINNDWGFTQYPVVPGHEVIGTVSAAGPEAKGVKVGQRVGVGWNAGCCMHCHECLT
                     GNHNLCIQAVPTIIGHYGGFAEKVRAHWLWAIPLPEKLEPGSAGPLLCGGITVFAPLV
                     NHGIKPTDRVGIVGIGGLGHMALQFINAWGCEVTAFTSNPAKSDELLKLGAHRIISSR
                     DSSEIRKAANSLDFLLVTVNIPLDWSAFLGTLKPRGCMHVVGAVLEPMPIPAFELISG
                     QKSVSGSPTGGPAAISQMLDFAGRHGIAPQVEHFPLSKVNDAIAHLVAGKARYRIVLD
                     ADFA"
     gene            complement(1971..2525)
                     /locus_tag="VFI43_04975"
     CDS             complement(1971..2525)
                     /locus_tag="VFI43_04975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7061514.1"
                     /translation="MDQYQIAVVVGSLRQESFNRHLANAVIRLAPPEFEFTHVRIDDL
                     PLYNQDNDGNQSEPVKRLKKEIASAQALMFVTPEYNRSIPGVLKNALDQASRPYGQNA
                     WARKPGGMMGASIGAPGTCMAQQHLRNILAYLDVPVLCQPEAFIQVKEGLFDEKGNIG
                     NADTKKFLQGWMDSYVAWVKRLAS"
     gene            complement(2809..>3206)
                     /locus_tag="VFI43_04980"
     CDS             complement(2809..>3206)
                     /locus_tag="VFI43_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HET7061515.1"
                     /translation="PLRLSSYLGLVDWPTIAGVDMRLISQHPAVNSLLGGLMLDDSRR
                     LYVAGIALSQLVSNVPAAILLADYSNDWTMIAYAVNIGGSGFILGSLANLIALRLAKD
                     GKTWLMFHAYSIPFLLITAALSYLWLFLA"
BASE COUNT          727 a          828 c          916 g          735 t
ORIGIN      
        1 ctgcccgggg aggtagcagc gtcactgtca tgaagcctcg caaccaccgt atcataaggt
       61 cccgctgtca gggccagggg taggccatag tctatctcat gcaagaaacc attgatgtag
      121 cccgaccacc cgacggcgac ggcggcggcg gccacggcat attcgagaat aagggcccag
      181 cccaccatcc atgccgtcat ttcgccgaat acggcatagg aatacgtgta tgcggagcct
      241 gctaccggga cgatcgcggc gatctccgag tagacaagcg cggtgagcgc gcaaacgaac
      301 ccggcgatgg caaaagagta catcatgccg ggtcccgcct tttgcgccgc gaccgatgtg
      361 agcacgaaga tcccggttcc gataatcgcg ccaatcccaa gcatggtgag atcgaatgct
      421 cccagttggc gcttgagccc ttttttttcc gccgattcga gtatgtgatc cagcgatttg
      481 atacgttcaa acagcaatat gcttctccgt attacggggg ccaaagggcg aactcgtaaa
      541 aactcaggct cttcgggtga ttatggaaaa agagtattct aaaccagagg gggcggtgtc
      601 gagtaactgg ctgagccgtt ataacattgg ttcaaaccac cagcgagctt gtcccaacca
      661 tggcttttca gaattcccgc ttcgagacga actgactcat tgtgggacgc gccattcgta
      721 tttcaaggca tgaaacgacg cgtggcgtat cattaaataa ccttattgaa ggagaacgat
      781 catggcgggt gtccgtagct gggcggtgca tggtgcgaag gagagactcg aaccattcgt
      841 ttatgaaccg gggccattgg ggctggagga ggttgagatc gccgtggaat actgcgggct
      901 ctgccattcc gatctctcga ttatcaataa cgactgggga ttcacgcaat atcccgtggt
      961 gcctggtcat gaagtgattg ggacggtgtc ggcggcgggt cccgaagcga agggcgttaa
     1021 ggtggggcag cgcgtggggg tgggctggaa cgcagggtgc tgcatgcact gtcatgaatg
     1081 tctgactggc aaccataatt tatgtatcca ggccgtgcca accattatcg gtcattacgg
     1141 aggttttgcg gagaaagtgc gcgcgcactg gctctgggcg attccgctgc ccgaaaagct
     1201 cgagcccggt tcagcaggcc cattactgtg tggcggcatt acagtatttg ctccgctcgt
     1261 taaccatgga ataaaaccga cggacagggt ggggattgta ggcattggcg gcttgggtca
     1321 catggcgctt caattcatca atgcctgggg ttgcgaggtg actgctttca cttccaaccc
     1381 agcaaaatct gacgagttgc tgaagctggg ggcgcacagg attatatcca gcagggatag
     1441 ctctgaaatt cgcaaggcgg cgaattcact cgattttctg cttgttacgg tgaacatccc
     1501 actcgactgg agcgcgttct tgggtacgct aaaaccccgt ggctgcatgc acgtggtcgg
     1561 cgcagtactt gaacccatgc cgatccctgc tttcgaactc atctccggac agaaaagcgt
     1621 atcaggctct ccgaccggag ggcctgcagc aatttcacaa atgctggact tcgctggacg
     1681 gcacggcatc gctcctcagg tggagcactt tcccctcagc aaggtcaacg atgcgatcgc
     1741 gcatctggtg gcgggcaagg cgagatacag gattgtcctg gatgctgatt ttgcctgagc
     1801 agtctgaagc gtcatcactt gctacccgtc acaccagtcg aatctccgcg gggccgtctg
     1861 cggccaaggg ataggaatta gcgcttattt tggtggaggt ccaaacggct gtttatcggc
     1921 tgtgtgtcgg ctgtggtcgg ccgtgtatca aactggtggt tgcaacacct ttagctcgca
     1981 agccgtttaa cccacgccac gtaactgtcc atccagccct gcaggaattt cttcgtgtct
     2041 gcgttaccga tgtttccctt ctcgtcaaat aaaccttcct ttacctgaat aaacgcttcg
     2101 ggttggcaaa ggaccggcac gtcgagataa gcaagtatgt tacgaaggtg ctgttgcgcc
     2161 atgcacgttc ccggagcgcc gatcgatgct cccatcatcc caccgggctt gcgtgcccag
     2221 gcgttctgcc catagggacg ggaagcctga tcgagtgcat tcttgagaac gcctggaatt
     2281 gatcggttat attcgggcgt aacgaacatc agcgcttgag ccgaggcgat ttccttcttc
     2341 agccgcttta cgggttcaga ctgatttccg tcgttatcct ggttgtaaag tggcaaatca
     2401 tcgattcgca cgtgcgtaaa ttcgaactcc ggcggtgcaa gcctgattac cgcgttggcg
     2461 agatgacgat tgaaggattc ctgacgcagg ctgcccacca ccacggctat ttgatattga
     2521 tccatgcgta actcctctgg tatatgctgc aattgcgtgt acttcagcag caggactgtt
     2581 atttagttag ttgttgatga tcgcctggaa ataggttctc taagaaagct tagattattc
     2641 gtcctgcggc gccggaaaat tacatgaggc agagcttttt ttgggaattg catctccgat
     2701 ttgcacgttt catcttcggt ctgtgatgaa agcctgtact tggcccgtac tttagggcgt
     2761 ctgcctgact ccccaaatgt gcttgaacgg cgtgactacg ggcaggactt aggcgaggaa
     2821 cagccacagg tacgagagag cggccgtaat caatagaaac ggtatcgagt acgcatgaaa
     2881 catcagccag gttttgccgt cctttgcgag gcgcagcgca ataagattcg cgagcgaccc
     2941 taatatgaac ccggacccgc cgatattgac tgcgtaagca atcatcgtcc agtcattcga
     3001 gtagtctgcc agtaaaatgg cagcggggac gttgctgaca agttgtgaaa gcgcaattcc
     3061 ggcgacataa agccggcgag agtcgtccag catcagtcct ccaagcaatg aattgacagc
     3121 cggatgctgg gaaatcagcc gcatatcgac cccagcaatg gtgggccaat ccacaaggcc
     3181 aaggtaagag gaaagccgca agggtt
//
LOCUS       DASSHJ010000124         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_910349, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000124 DASSHJ010000000
VERSION     DASSHJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910349"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFI43_04985"
     CDS             complement(<1..678)
                     /locus_tag="VFI43_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET7061516.1"
                     /translation="MQIEISAKKRELQGKGASRRLRGSGQVPGVVYGGERPAQAIELD
                     HNNLYHQLKQEAFHASILTMDMEGQKEPVLLRDIQMHPFKLQVLHIDFQRIDPNKKIH
                     MKVPLHFINADIAPGVKLSGGIVTHILTELDVSCLPKDLPEFITVDLSDLTAGNTLHL
                     SDLKMPEGVEIPALLKGEDLSVATIVIPRGVAAEEGAAVAASDIPTTVQKKEGVSKEG
                     EKKDAGKK"
     gene            complement(817..1767)
                     /locus_tag="VFI43_04990"
     CDS             complement(817..1767)
                     /locus_tag="VFI43_04990"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7061517.1"
                     /translation="MAYDSLMVFTGNANPKLAQDVVRHLNIRMGRAAVGRFSDGEVMV
                     EILENVRGKDVFVLQSTCMPTNDTLVELLVLVDALKRASAGRITAAIPYFGYARQDRR
                     PRSVRVPITAKVVANMLTTVGIDRLLTMDLHSDQIQGFFDIPVDNIYSMPILLGDIWK
                     NNYQNLVVVSPDVGGVVRARQMAKRLECDLAIIDKRRPKANVAKVMNIIGEVDGRTCV
                     IMDDMVDTANTLCEAARALKEHGAERVVAYCTHPVLSGNAVERIENSALDKLVVTDTI
                     PLREDARACSRIHQLSVASLFAETMLRISNEDSVSSLFME"
     gene            complement(1872..1946)
                     /locus_tag="VFI43_04995"
     tRNA            complement(1872..1946)
                     /locus_tag="VFI43_04995"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1912..1914),aa:Gln,seq:ttg)
     gene            complement(1950..>2511)
                     /gene="ispE"
                     /locus_tag="VFI43_05000"
     CDS             complement(1950..>2511)
                     /gene="ispE"
                     /locus_tag="VFI43_05000"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7061518.1"
                     /translation="GGLGGGSSDAATTLIALNRLWGLDWKRKRLQKLGLGLGADVPVF
                     IFGENAFAEGVGEELRTIDLPPAWYLVLTPPVHVSTAEVFASKELTRNTIPIKIPPFS
                     VERGHNDLEPVVCGLYREVAHALQWLRQLHAARIAAMTGSGACVFAEFATETAARLAL
                     AQVPSGMSGFVAEGLSRHPLHDLVEG"
BASE COUNT          534 a          761 c          643 g          573 t
ORIGIN      
        1 tttctttcct gcgtccttct tttcaccttc cttggaaacg ccctctttct tctgtacggt
       61 cgtcgggata tccgatgcag ccacagccgc cccttcttcc gcagccacac cccgtgggat
      121 gacaatagtt gccaccgata aatcctcccc cttgagaaga gcaggaattt ccacgccttc
      181 cggcattttc aggtcgctca ggtgaagggt atttcccgcg gtcaggtcag acagatcgac
      241 ggtgatgaat tcgggcaggt cctttggcag gcatgaaacg tccagttccg tcagtatgtg
      301 ggtgacaatg cctccagaga gctttacgcc cggtgcgatg tcagcattga tgaagtgcaa
      361 gggcactttc atgtgtattt tcttgttcgg atcgattcgc tgaaaatcga tatgcagcac
      421 ctgcaatttg aagggatgca tttgtatgtc gcgtagcaga acgggttctt tttggccctc
      481 catatccatc gtcagaatgg aagcgtgaaa ggcttcctgc ttgagctggt ggtaaaggtt
      541 gttatgatcc agttcgatag cctgggcagg gcgctccccg ccgtaaacga ctcccggcac
      601 ctggccggaa ccacgcaggc ggcggctcgc acctttgccc tgcagttcgc gctttttcgc
      661 gctgatttct atttgcatca ttttctccag agtgtggatc gacccgcgac caggtgaccc
      721 gattgacaat aaaccgaacc tttaatatct gatacttcca aacgccctac tgatgctgct
      781 tcactattat tacggttccg gcgccctacg ctaccgctac tccatgaaca gcgaactcac
      841 cgaatcctcg ttgctgatcc tcaacattgt ctccgcaaaa aggctggcga cactcagctg
      901 atggatgcgg gaacacgccc tggcgtcttc ccggagcgga atggtatcag tcaccacgag
      961 cttgtccagg gccgagttct cgattcgctc caccgcattg ccggacaata cagggtgagt
     1021 gcaataagcc accacccgct ccgccccgtg ctctttcaag gcgcgcgcag cctcgcatag
     1081 cgtgttggcc gtatctacca tgtcgtccat gatgacgcag gtgcgcccat ccacctcgcc
     1141 gatgatattc atcactttgg cgacgttggc tttgggacgc cgcttgtcga taatggcaag
     1201 gtcgcattcc agccttttgg ccatctgcct cgctcttacc accccgccca catccggcga
     1261 gaccactacc aggttctggt agttgttttt ccatatatcg cccagcagga tgggcatact
     1321 gtagatattg tcaacgggaa tatcgaaaaa tccctggatc tggtccgaat gcagatccat
     1381 ggtcaatagc cggtctatcc cgaccgtcgt cagcatgttt gccaccacct tggccgtgat
     1441 cggcacacgg accgagcgcg gccttctgtc ctggcgagcg tatccaaaat agggtattgc
     1501 cgccgtaatc cggcccgccg aggcccgttt cagcgcgtcg accagcacca gcagttcaac
     1561 cagcgtgtca ttggtcggca tgcaggtcga ttgcagcacg aacacatcct tgccgcggac
     1621 gttctccagg atttcaacca tcacctcgcc atcgctgaag cggcctacag ccgcccgtcc
     1681 catccggata ttcaggtgac gtacgacatc ctgcgccagc ttggggttgg cgttgccggt
     1741 aaaaaccatc aagctatcgt aggccatggc tggaggctat gtattgggat tcagagcgga
     1801 ttgaggaaca tttcctcgct tcgggaacta ctcgtgattc gataagacct cgaaactaca
     1861 cggcttgaat atggctgggg aggtagggat cgaacctacg aatgccggaa tcaaaatccg
     1921 gtgccttacc acttggcgac tccccagaac tagccttcca ctaaatcatg caggggatga
     1981 cggctcaatc cctccgcaac aaaaccgctc ataccggatg gaacctgcgc cagagctaac
     2041 ctcgctgccg tctccgtggc aaattccgca aatacgcaag caccggagcc cgtcattgcc
     2101 gcgatgcgcg ccgcgtggag ctgcctgagc cactgaagag catgggccac ctcacgatat
     2161 aagccgcata ctacgggctc gagatcgttg tgtccgcgct cgacggaaaa gggcggtatt
     2221 ttgattggaa tcgtgtttcg tgtcaattcc ttactggcaa aaacctcggc agtcgagaca
     2281 tgcacgggtg gcgtaagcac gagataccag gcggggggca ggtcgatagt gcggagctct
     2341 tcgcccaccc cttccgcaaa agcgttctcc ccgaatataa agacgggaac atcggcgccc
     2401 agccccagcc ccagcttctg caatcgcttt ctcttccagt ccaagcccca taaacgattg
     2461 agagcaatta gcgtagtggc agcatccgaa ctgcctcccc ccaaccctcc c
//
LOCUS       DASSHJ010000125         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_913668, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000125 DASSHJ010000000
VERSION     DASSHJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913668"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFI43_05005"
     CDS             <1..646
                     /locus_tag="VFI43_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET7061519.1"
                     /translation="GFEYSKWFGRDAAEHLVHSLEDISRSAQPGETPVVSIILDGENA
                     WEYYPYNGYYFLNDLYEIIENHEVIRPTTYRDHIASMIPSDAAELPVLAAGSWVYGTF
                     STWIGDKDKNRAWDLLCAAKHSYDLVIQSGRLTDDEIARAERQLASCESSDWFWWLGD
                     YNPPLIVESFDKLFRANLANLYTLLKLPVPLPVLEPISQGGGAPESGGTMRRAS"
     gene            832..2859
                     /locus_tag="VFI43_05010"
     CDS             832..2859
                     /locus_tag="VFI43_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HET7061520.1"
                     /translation="MPQPVSLLFGVHAHQPIGNFPEVLADAHLRCYRPFLHVVYRYPR
                     FRFAFHVSGWLLDYLIEHYPEDMVLLREMVARGQVELFGAGDTEPVLAAIPHRDRIGQ
                     VQGFSDKLETKLGQRPRGAWLTERVWDSTVVPALADCAIRYVIVDDYHFLCAGKQADE
                     LNSYYTTEEDGRTLDLFPISEALRYRIPFSPAEEVVAYVESLAGRATGGGAAIYFDDI
                     EKFGIWPETYQWVYENGWLDKFIQGVLGSPIINAEHYGDYHSATKTRGIVYLPTTSYI
                     EMNEWTLPAGPARAYSALVHHAKAEGWYESNKAFLRGGIWRNFFSRYPESNWMHKRML
                     SLSRRLQSLPDDQRTPAMKRKLYESQANDAYWHGLFGGLYLPHLRRAVYGAIVELEAM
                     LDAYASRPARFIEDTDLDGFDELYLQNGVIQAVLKLDGSGSACELDSYLLKHNFGDTL
                     RRQTEHYYHKMHEGQNREQAGGGIASAHDRVSFKHKIDAQDITVDEHGRGLFMDRLNG
                     AAVDYQHKPLPNRGSYCHVEVGSPQIVKSFELVDNKLLASYHFLEGVEGIFSTEINLA
                     MPSCDGWGGRYVYKGDIPGGFGQTLELDGISEITLDDDELGGSIELKVSHPALLRGRP
                     YYSVSQSEAGFEKIMQAVTLVLEWQETIFEDLVITLEVGSKEKPEILRFLG"
     gene            3052..>3330
                     /gene="fdxA"
                     /locus_tag="VFI43_05015"
     CDS             3052..>3330
                     /gene="fdxA"
                     /locus_tag="VFI43_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HET7061521.1"
                     /translation="MTYVVTEACIKCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCT
                     LCVAECPVEAIYSEDDVPVNQQQFTALNAELSKAWKPIIEKKDALPEAD"
BASE COUNT          741 a          824 c          969 g          796 t
ORIGIN      
        1 cggcttcgag tattcgaaat ggtttgggcg ggatgctgcc gagcatctgg tgcattcgct
       61 cgaagatatc agccgaagcg ctcagcctgg ggaaacgccg gtggtaagca tcatactcga
      121 cggtgaaaat gcctgggaat attacccata taacggctac tatttcctca acgacctcta
      181 cgaaattatc gaaaatcacg aagtgataag accgacaact taccgggatc atattgcatc
      241 gatgattcca tctgatgcgg ctgagctgcc tgttttggcc gccggcagct gggtatacgg
      301 gaccttttcg acctggattg gcgacaagga caaaaatcgg gcgtgggatc tgctgtgcgc
      361 ggcgaagcat agttacgatc tcgtgattca gagcggccgc cttaccgacg acgaaattgc
      421 aagggccgaa cgtcagcttg cttcctgcga aagttctgac tggttctggt ggctgggtga
      481 ttacaatcct ccgctcatcg tagagagttt cgacaagctg tttcgggcca atctggctaa
      541 tctttacact ctgttaaagc tgcccgtgcc gttgccggta cttgagccca tcagtcaggg
      601 gggtggggcg ccagagtcgg gcgggacgat gcgccgcgcc tcctgaggac gaagaagtga
      661 tggtcttccc gtactccaag ggggaattcg aataatcaaa gaccgcgctt ttcaggccca
      721 ggagcgggtt ctgattcgcc aggacttatc tggcatatag cgagttcccg ttccatacaa
      781 gaaagcggtg tctcggcacc gctgcgcgat gctctttcgg agaatggctt catgcctcaa
      841 cctgtctctc tgctgttcgg cgtacatgct catcagccca tcggaaactt cccggaggtc
      901 ctggccgatg ctcacctacg ctgttacagg ccgtttctcc acgttgttta ccgctatccc
      961 aggttccgtt tcgccttcca cgtttccgga tggctgctgg attatcttat cgagcattat
     1021 cctgaagata tggtgctgct gcgggaaatg gttgcgcgcg gccaggtcga gctgtttggt
     1081 gcaggagaca cggagcctgt actcgctgca attccgcatc gggatcgtat cgggcaggta
     1141 caagggttct cggacaagct ggagacgaag ttggggcaac gcccgcgagg agcgtggctg
     1201 acggagcggg tctgggattc gaccgttgtc cctgcgctgg ccgattgtgc cattcgctac
     1261 gtgatcgtgg atgattacca ttttctatgc gcaggcaagc aggcggatga actgaacagt
     1321 tattacacta ccgaggagga tgggcgtacg ctcgacctct ttccaatctc ggaagccttg
     1381 cgttaccgga tcccgttttc gcccgccgag gaggtggtgg cgtacgtgga gtctcttgca
     1441 ggcagggcga cgggaggcgg ggcggcaatt tactttgacg acatcgagaa attcggaata
     1501 tggcctgaga catatcagtg ggtatatgaa aacggttggc tggataaatt catccagggt
     1561 gttcttggtt cgccgatcat caatgcagag cattacgggg attatcattc cgccacgaaa
     1621 acccggggga tcgtctatct gccgacgact tcctatatcg aaatgaacga gtggaccttg
     1681 ccggcgggac ctgcccgtgc ttattccgcc ttggtgcatc acgccaaggc cgaaggctgg
     1741 tacgaaagca acaaggcctt cctgcgtggc ggcatctgga ggaatttctt ttcccgatat
     1801 cctgaatcca actggatgca taagcgcatg ctgagtcttt cccggcgcct tcagtccctt
     1861 ccggacgatc aacgtacccc tgccatgaag cgaaaactat acgagtcgca ggccaatgac
     1921 gcgtattggc acggtttgtt cggagggctt tatctgcctc atctgcggcg tgccgtgtat
     1981 ggcgctattg ttgagctgga ggcaatgctg gatgcgtacg cgtcccgccc cgcgcgattc
     2041 attgaagata ccgatctcga tgggttcgat gagttgtatt tgcagaatgg cgtaatacaa
     2101 gcggtcctga aactggacgg aagcggaagc gcatgcgaac tggactctta tctgctgaag
     2161 cacaactttg gcgatacgct gcgacgtcag acagaacatt attaccacaa aatgcatgag
     2221 gggcagaaca gggagcaggc cgggggcggg atcgcctcgg cgcatgacag ggtgagcttc
     2281 aagcacaaga tagatgcgca ggatattact gttgacgagc atgggcgcgg cctgtttatg
     2341 gatcgcttga atggcgcagc agtcgattat cagcacaaac cgctgccgaa tcgcggttcg
     2401 tattgccatg tggaagtcgg ttcccctcaa atcgtaaaga gtttcgaact ggtcgacaac
     2461 aagttgctcg cctcatatca tttccttgag ggcgttgaag gtatcttcag taccgaaatc
     2521 aatctcgcca tgccgagttg cgatggctgg ggcgggcgtt atgtttataa gggagatatc
     2581 cccggggggt tcggacaaac gctcgagctg gatggcatct cggaaataac cctggatgac
     2641 gatgaactcg gtggaagtat cgagctgaaa gtatcacatc cggcgctttt gcgcggccgg
     2701 ccctattact ccgtgtccca atccgaagcg gggtttgaga agatcatgca agcggtgaca
     2761 cttgtcctcg aatggcagga gacgatcttt gaagatctgg tcatcaccct cgaggtcggg
     2821 tcgaaagaga aaccggaaat ccttaggttc ctgggttagg caggtgcgtc gctacaagct
     2881 ggccctatct ttctccattc tttgatggct tagaacgagg cacgaataaa cgtgtttcgg
     2941 ggagagtagt cggaattgag taccaatgtg cgagtctgta gagttgtagt gtaggctgta
     3001 atatttctgg taaaatcgcg aattgtccaa gagttcagca agggatctgt catgacctat
     3061 gtagtaaccg aagcctgtat caaatgcaaa tacactgatt gcgtggatgt ctgcccagtg
     3121 gattgctttc gcgagggacc gaattttctc gtgattgatc cggacgagtg cattgattgt
     3181 accctttgtg ttgctgaatg cccggtcgag gccatttact ccgaggatga cgttcccgtg
     3241 aatcagcagc aatttaccgc actcaatgcg gaactatcca aggcctggaa gcctattatc
     3301 gagaaaaaag acgctctacc cgaagcagat
//
LOCUS       DASSHJ010000126        10929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_667918, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000126 DASSHJ010000000
VERSION     DASSHJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667918"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..2183
                     /locus_tag="VFI43_05020"
     CDS             48..2183
                     /locus_tag="VFI43_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061522.1"
                     /translation="MYNKNSSAKWPLLAVTMWLGSMVVGGAAFADNAVKLRVLLVTTG
                     DLTQDIGFAYIKPALDEMGVPYDVLNAGTQDLTAEMLAAKSGVACTAATAGCIGNYNG
                     IILTDSDLVPNFTPAEWDILHNYEKSFAVREAVLSGWPATYWDPNAPFGIYLDYGLVY
                     SSSASNYTGAWSIPVAYTRQVFEYVNSANTLPITDFAFSANPRNDATLLRDGTKPNVV
                     PLLKTQNGEALVSIVQYVVPPQTTPVREVMISTITNADFLVHSKVLVYEFINWATQGV
                     FVGARYIHMAAHADDLFIPDELWDPALKATNPSTTFRLGSADINNAVSKQAAFRAAHP
                     VAGTFKLDFPFNGSGAVLDPEAIALAADLSDDLVAAVVANKANFRFINHTFTHADMDK
                     APVPANAPCDYETFTTISATQAEITKNRTVWGLLALPEQSQNNRMLLSGNHSGLKDRK
                     CTADPALHPEMADVQSDDVAFDQGGANPLFLQAAARASVSYLASDSSQRAQNIEQYIS
                     QYNDGSQTDRLMLPRWPTNIFYNVINPTQQVDEYNYIFHDRFVSGGQDPCQIPGAICS
                     PRSYADILLAEADTAIRHMMTFNKWPHFFHQTNVASYDASGNTLQFDWLNAVFTEYER
                     LFTLPVKNEPHYLIGDHTADSLTAKSAAIQAVWNRTTNQVTLSANKAVPNLLVTGLSG
                     GELYGGQYIRTISINTTAKAIMVNRGLTQ"
     gene            2240..2641
                     /locus_tag="VFI43_05025"
     CDS             2240..2641
                     /locus_tag="VFI43_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061523.1"
                     /translation="MEYNIHSTRTFLALLAVAVTIGTGTPLAVYAGAGQADGKPQHAN
                     DENNRKKIREANDVKLRAQEPFYSEHAKALAEQYRETAEIVARQGGNAQPILDAAAHL
                     ESQSELVAKVRRNAVPIEIPQPVSHPKVHKK"
     gene            2923..5094
                     /locus_tag="VFI43_05030"
     CDS             2923..5094
                     /locus_tag="VFI43_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061524.1"
                     /translation="MLFVSITGDRTFMKEKPDISSLLVPVTMWLALMTAATPTFADNA
                     VKLKVLIITTGDVAQDPGYAYIKPVLDEMGVPHDVLNAETQDLAAEMLASSAQGACKA
                     AEAGCVGNYNGIILTDSDLVPNFSPAEWEILHGYEQDFGVREAVLSGWPGTYWDPNPP
                     YGIYLDYGLVFSSAGKLYDSKWTVPEAYSKQVFEYVNQANDLRITDFAFAATPRKEIQ
                     TLRDGTVPKVEPLLRTQNGEALVSVVRYMTPVKTTPVREVMISTITNSPQPLHSKVLA
                     YEFINWATQGIFVGARFVHMAAHLDDLFLGDHLWDPSVKGNNLANIYRLNGTDIDNAV
                     SKQGDLIATHPLAGGFKLDFPFNGYGAVVDPTAMTLATNLADELVSAVIANKNNFRFI
                     NHTFSHRLMDKTPVPAEAPCDYATFTDMTAVQAEISRNRTVWSLLGLPEQNENDEVLI
                     SGNHSGLKDNKCTIYPAMHPDMPNIQADDVGFDQGGANPLFLQAAASLGVKYLASDSS
                     QQGQNIEQYITGYDDGSQDDRVMLPRWPTNVFYNATNPTLLTDEYNYLFHDRFVSAGQ
                     DPCATPGAICSPLSYTEILGVEADKALRRMLTFSKWPHYFHQSNLAKYDESGSTVLFD
                     WLNAVFTAYERLFTLPVKNYPYYLIGRRTAESLDAKSTTVEAVWNRTTNEVTLSAGKA
                     LSGLLVTGLSGGELYGGQSIRKVNLDINGIATYPVDRALTQ"
     gene            complement(5175..8753)
                     /locus_tag="VFI43_05035"
     CDS             complement(5175..8753)
                     /locus_tag="VFI43_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7061525.1"
                     /translation="MNLFGSLKKTIFLFCLGVILVFALLFYKEDRLKELLQVERPDHL
                     SVLYLQLLLNMNPEDVGLRIQLARHYINMGELAEARTALEPLLARNGPGELDTRLLAL
                     EIDFKDYYSTGEDNPSRKTKLATLQNSVAEISRNQVPVALLPKVIKLNLELEQPSVVA
                     DLYYRWSESADDSSDRVAKLKESARWHMASEQPRKAADIYHECYELSEDVEQARQFAF
                     LTLEALRAAGESQIALQYLGKYQQRFPEDPELLDEAINIYLASNDQRKAYETGAQRLA
                     LEPENTEQIRKQADRALAVGELQSAVALAQRIVDLAPADENAHENLGRIAEWALMREV
                     ALKEWLWLARNRKDEPSIINTIRLSNALNSTGTALEMLIQLSGMRKLTNDEMNSALSA
                     YAEAGSLTEHVNFLKSYLDRYPDNPQVWEALAKTQENAGQLAEAIVTWRHIGGEFNRL
                     PEALAHQGRLIWKNGQSEKALAVLVANKDKVTEKDLPFWEVLGDISWEMKRPEHALGA
                     YRILWKSGSTSAIVAERLIQLTRDMSKWTESIAVGEEGYQRLNQPRWLLLSMDVANQA
                     GLAEQLKRLFKIAEKNESQFRDSEMYWLMHAQLNLNEKEPETALKDYHQALKVNPASA
                     TAKEGILWNLIGSDDKESLQAHIKTWRSEASKNPSLWGVYGVALTKVGQNKEALPWLE
                     RKTKANPQDYLWLLTYADTLGKSGHADKAWRLRKYILSSLRSRLNEVAKAPDKKIKEL
                     LRPEYFALIRDLEGANADVSVLKKLLAKGYDDPAVQELLVASYLSQENYPAARYWLLK
                     EHISRQQSPTWQRLALALGENDLAAVENILEKENDKLSEFNRMEAFRRLERNDEALAS
                     TYKLLELHKQPSSLQSGLFNLRDDLVVKTSRQIATSFDFRTLGNINFLEGRTRFNMPY
                     MRGVLATDLKYINLGSTDPNVRLPATNEVDISLEYKHPLRQGTFQANVGGNLREVNSI
                     PYGAVRLTQDITQRVRASLRAGVSELSHETGALRALGKKDTLLLGVSAQLSQQMFANF
                     EADGHRYSSRGGGELGKGYKLQGILGYSLLSGIQDWQVRLQGSWENNHLAQTLPSDLD
                     GLLSPSVAQPATIVPRRFAMMGMGTSFRYGPPDQGILRRPFVLADVWSGYVWPADAIG
                     YNGRISMGISLLGRDILSVSAFYSNVQGGRTNQPFAGAGIQYSFRF"
     gene            complement(9198..>10929)
                     /locus_tag="VFI43_05040"
     CDS             complement(9198..>10929)
                     /locus_tag="VFI43_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061526.1"
                     /translation="LRPEVYREWLLRQMQSGVKVVVLGNLGFKADNSFLHHLGVQSSQ
                     SESSVEPGSMVFNDRIIGYEAQPAPGTPGFPLWRTLDGSTEKHLSWGEQPETPLVAVF
                     SGQWGGVALHPYVVAHGHQGRQRWIINPFEFLSTALDLSGIPVPDVTTENGRRLLLVQ
                     IDGDGAGAKAEIDGRPTAMKVIQHRFLQKYPLPSTVSIIEGEIANGERSGGLSEAEKT
                     ARDVFKMNNVEIASHSYSHPSSWFAKNNISTVGDDYRLPISGYGFDIQREIAGSVEYI
                     NKTLAPEGKDTRVFLWTGDGLAGKDALSLVHSLGLENMNGGGATISSDEKTVTRVPPT
                     GYSIDGQIQVYAPIASDAFYINRRQEPGFTGVIDTMQLTDNPLRLKPMHIHYHFYSGI
                     KITSTDGLKSVYDWSVKQEQRPVWVSEYSQKVNEFYNITLSRRLDGAWDIRGVDKLRT
                     LRLPVSTGWPDLQRSEGIVGVHDVNQGRYVHLFPKNGRVLLYTSTDPPSSIHLLHSNG
                     EIEEWQETAEGVNFQVRSHVPLEMVTGPPGQKCYVNWSGGRLDGQHENKGWKFIFPVA
                     DPGNATLVCY"
BASE COUNT         2601 a         3005 c         2715 g         2608 t
ORIGIN      
        1 atatcagggc cttttttgtt ttcgttagga aaaaggagat tttactcatg tacaacaaga
       61 attcgagtgc gaaatggccg ttgctcgcag tgacaatgtg gttaggctcg atggttgtgg
      121 gaggggctgc gtttgctgat aacgccgtta aactcagggt actgctcgtt acgacaggcg
      181 atttgacgca ggacattgga tttgcctata tcaagcctgc ccttgatgaa atgggcgtgc
      241 cctacgacgt attaaatgcc ggcacccagg atctcaccgc tgagatgctc gcagccaaga
      301 gcggggtagc ctgcaccgct gccaccgcag gctgcatagg caattataac ggcatcatcc
      361 tgaccgattc cgacctggtt cccaacttca cacccgcgga atgggacatc ctgcacaact
      421 acgaaaagag tttcgcggtt cgcgaagcgg tactgtcagg atggccggct acttattggg
      481 atcccaatgc ccctttcggc atctacctgg attacggctt ggtctattcg tccagtgcga
      541 gcaattacac tggtgcgtgg tcgattcccg tcgcttacac caggcaggtt ttcgaatatg
      601 tgaactcggc taacaccctt ccaattacgg actttgcctt ctcggccaat cctcgaaatg
      661 atgccaccct cttgcgggat ggtaccaaac caaatgttgt gccgcttttg aaaacccaga
      721 atggcgaggc gctggtatcg atcgtgcaat acgtggtgcc cccgcaaacc acaccggtgc
      781 gcgaggtgat gatctcaacg attaccaatg cggatttcct tgtccactca aaggttctgg
      841 tctacgaatt catcaattgg gccacgcaag gggtttttgt cggggcacgc tatatccata
      901 tggctgccca tgcggatgat ttgttcattc ctgacgaatt gtgggatcct gccctcaagg
      961 caaccaatcc tagcaccacc tttcgcttgg gcagcgccga catcaacaat gcggttagca
     1021 agcaggccgc ctttcgcgct gcacatccgg tagcgggcac gttcaagctc gattttccgt
     1081 tcaatggttc cggtgccgta ctcgatcccg aagcaatagc gcttgccgcc gacctgagcg
     1141 atgacctggt ggccgcggtg gtcgccaaca aggcaaattt ccgttttatc aatcacacct
     1201 ttacgcacgc agatatggat aaggcacccg ttccagcgaa tgccccctgt gattacgaga
     1261 cctttaccac catctcggct acccaagctg aaattaccaa aaaccggaca gtgtggggac
     1321 ttctggctct tcccgagcaa agccagaata acaggatgct cttgagcggc aaccactctg
     1381 gtctcaaaga ccgcaaatgc acggcggatc ctgccttgca tccagaaatg gccgatgtcc
     1441 agtctgatga cgtggccttt gaccaggggg gcgcgaatcc gctcttcctt caagccgccg
     1501 ctcgcgcatc agtgagctat cttgcctctg actcgtccca acgcgcccaa aacattgagc
     1561 agtacatcag ccagtacaac gacggcagcc agacggatcg attgatgctc ccgcgctggc
     1621 ccaccaatat tttttacaat gtgataaatc cgactcagca ggtggacgag tacaactata
     1681 tctttcatga cagattcgtg agtggtggac aggatccttg ccagataccc ggagccatct
     1741 gctctccgcg gagttatgcg gatatcctgc tcgccgaagc ggatacggca atacggcata
     1801 tgatgacctt caacaagtgg ccgcattttt ttcatcaaac gaacgttgcc agctatgacg
     1861 cgagcggcaa cacgctgcag ttcgactggc tgaatgctgt ctttaccgag tacgagcgac
     1921 tgttcacgct gccagtcaag aacgagcccc attatttgat cggcgatcac accgcagaca
     1981 gcctcactgc caaatccgcc gcgattcaag ccgtctggaa ccgcaccacc aatcaggtca
     2041 ctctatctgc aaataaagcc gtccccaatc ttttggttac gggactctcc ggtggagaac
     2101 tatatggcgg tcaatatatt cgcaccatta gcataaatac caccgccaag gcgattatgg
     2161 tcaaccgggg gctcacgcag tgacatcgac cagcattctg gaatctttcg aaaacgtaaa
     2221 agatcaagag gattttcaaa tggaatataa cattcactcg acgagaacgt ttcttgccct
     2281 gctggccgtg gccgttacaa tcggcaccgg aacaccgctc gcggtatatg ccggggcagg
     2341 gcaagctgat ggcaaaccac agcacgccaa tgatgagaat aacaggaaga aaatacgaga
     2401 ggcaaacgac gtgaagctga gggcgcaaga gcctttctac tcagagcatg ctaaagccct
     2461 tgctgagcaa tacagggaaa ctgcggagat tgttgcacgt cagggcggta acgcccaacc
     2521 aattctcgat gccgctgctc atcttgagag ccagtctgag ctggttgcga aagttcgccg
     2581 gaacgcggtc cctatagaga ttccgcaacc tgtatcccat cccaaggttc ataaaaaata
     2641 gctcgtcgag aagcaccgac gattgcctag gcaagccgga gacaatcctc ccttggcaca
     2701 gggggcgtct gaatagccca cccaaaaggg ggaggaaccg gcttttcgtc ttcaatatcg
     2761 cacccatgcg tttgcgattg ctctagccac ggtccagcgc ctctttcccg acccctacat
     2821 tgggcccctc taggctgccc gaaaccatgc agactaataa tgatttttac agctaatgaa
     2881 agtaaggaga agaccgcaaa gaattaaggg atgcttttcg gaatgctttt cgtctcgatt
     2941 accggagaca ggacattcat gaaagagaag ccagacatca gctcgctgct tgtaccggtg
     3001 acaatgtggc tggcgttaat gactgcggct acccccacat tcgccgataa cgccgtgaaa
     3061 ttaaaggtcc taattatcac gacaggcgat gtcgcgcaag atcccgggta tgcgtatatc
     3121 aagccggtcc tggatgagat gggcgtcccc cacgatgtgt taaatgctga gacgcaggac
     3181 ttggcagccg agatgctggc atcctcggcc cagggtgcct gcaaggctgc ggaggcgggt
     3241 tgcgtgggaa actataacgg gatcattctg accgactcag acctcgtccc taactttagt
     3301 cccgcggagt gggagatcct gcacggttac gaacaggact tcggtgtccg cgaggcggtt
     3361 ctatcaggat ggccgggcac ttattgggac cccaatcctc cctacggcat ttatctggac
     3421 tatggcttgg tgttttcgtc agccggaaaa ctctacgata gcaagtggac cgtacccgag
     3481 gcatacagca aacaggtttt cgagtatgtg aaccaggcta acgacttgcg catcaccgat
     3541 tttgcctttg cggccacccc ccgaaaggaa attcaaaccc ttcgggatgg gaccgtgcca
     3601 aaggtcgagc cgctgttaag aactcaaaac ggcgaagccc tggtatccgt tgtgcggtac
     3661 atgacgcctg taaagaccac gccggtccgc gaagtcatga tttcaacgat tacgaacagc
     3721 ccacaacccc tccactcgaa ggttctggcc tatgagttca tcaattgggc cacccagggg
     3781 atattcgtgg gagcgcgctt tgttcatatg gctgcccatc tggacgattt gtttctgggc
     3841 gatcatttgt gggacccgtc agtcaagggg aataatctgg cgaatatcta tcgcttgaac
     3901 ggtactgata tcgataatgc ggtaagcaag cagggcgatc tcatagcaac gcatccatta
     3961 gcaggtgggt tcaaactgga ttttccattc aatggttatg gggcagtggt agatccaaca
     4021 gcgatgacac tggctacgaa cctagcggat gagctcgtga gcgcagtaat tgcaaacaag
     4081 aacaatttcc gcttcatcaa ccacaccttt agccaccggc taatggacaa gacgccggtt
     4141 cccgccgagg cgccttgtga ttacgcgacc tttacggata tgacagctgt acaagcggaa
     4201 atcagccgaa accgaacggt ctggtcgctc cttggccttc ccgagcagaa tgagaacgac
     4261 gaggtgctta taagcggtaa tcactcgggg ttgaaagata acaaatgcac aatctaccct
     4321 gctatgcacc ccgacatgcc caacattcaa gctgacgatg ttgggtttga tcagggtgga
     4381 gcgaatccgc tttttctcca ggccgccgcc agcctcggcg taaaatacct tgcgtccgac
     4441 tcatcgcagc agggacaaaa tattgagcaa tatatcactg ggtatgatga cggaagccag
     4501 gacgatcgag ttatgctacc gcgctggcca accaacgttt tctacaacgc tacaaatccg
     4561 actctgttga cggatgaata caattatctc tttcatgata gattcgtctc tgcagggcag
     4621 gacccatgcg caacacccgg agccatctgt agccccctct catacacaga aatccttgga
     4681 gtcgaagccg ataaggcatt acgccgaatg ctgacctttt ctaagtggcc gcattatttc
     4741 caccaatcga acctcgccaa gtatgatgaa agtggcagta ccgtcctgtt tgactggctg
     4801 aatgcagtgt tcacagcgta tgagcgattg tttacgctac ctgtcaagaa ttatccctac
     4861 tacctcatcg gtaggcgcac ggcggaaagc cttgatgcaa aatcgacgac ggtcgaggcg
     4921 gtttggaatc gcacgaccaa cgaggtgacg ctctcagcag gaaaagcttt atcaggcctt
     4981 ctcgttacag gtctttccgg gggagaactg tacggaggtc aatctatcag gaaggtaaac
     5041 ctggatataa atgggatagc gacttatccc gtagaccggg ctttgacaca gtaagtaagc
     5101 gatgagcaaa gtgttcctgt cggacacaca tcgccattta accctcgacc cgtctgcttg
     5161 cccgggtaaa agcgtcagaa gcgaaaggag tattggatac ctgcacctgc aaagggttga
     5221 ttggtccggc caccctgaac attactataa aaggcgctga cgctaagaat gtcgcggcct
     5281 agtagcgaaa ttcccattga aatccgcccg ttatacccta tcgcatcggc gggccacaca
     5341 tacccgctcc agacgtctgc aagaacgaag ggtcgcctca ggattccctg gtcgggaggg
     5401 ccatagcgga aactcgtccc cattcccatc atcgcgaatc gccgaggaac gattgtggcc
     5461 ggttgcgcca cggatggaga cagaagcccg tccaggtcag acggaagcgt ttgagcaagg
     5521 tggttgtttt cccatgaccc ttgcagacgg acctgccagt cctggattcc ggagaggagc
     5581 gaatatccca aaatcccttg cagcttatag cccttgccca gctcaccgcc gcccctggac
     5641 gaatagcgat ggccatcggc ctcaaaattg gcaaacattt gctgcgatag ctgagcggat
     5701 acgccgagca ggagcgtatc tttttttccc aaagctcgta atgcgcccgt ttcatggctc
     5761 agttcgctca caccggcacg aaggctcgcc ctcacccgtt gagtgatgtc ctgcgtgagc
     5821 cggaccgcac cgtagggaat ggagttcact tctctaagat tgcctccgac attcgcctgg
     5881 aacgtcccct ggcgcagtgg atgcttatat tccagagaaa tatccacttc gttggtggca
     5941 ggcaagcgga cattggggtc ggtggaacca agattgatat acttgaggtc ggtggccagt
     6001 acgccacgca tgtaaggcat gttaaagcgg gttcggcctt cgaggaaatt gatgtttccc
     6061 agagttctaa aatcaaagga ggttgctatc tgccggctgg ttttcaccac taggtcatcc
     6121 ctcaggttga agaggcccga ctgcagcgat gagggttgtt tatgcagctc gaggagctta
     6181 taggtggacg caagggcctc gtcgtttctt tccaaacgcc tgaacgcctc cattctgttg
     6241 aattccgaaa gcttgtcatt ttctttttca agaatgttct cgacagcagc cagatcattc
     6301 tctcccaatg ccaaagcaag gcgctgccag gtcggcgatt gctgcctcga aatatgctcc
     6361 tttagcaacc agtagcgagc ggcaggataa ttctcctggg acagatagga agccacgagc
     6421 aattcctgaa cggcaggatc gtcgtatcct ttggcaagca gcttcttcaa tacggataca
     6481 tccgcatttg caccttccag atctcgaatc aaggcaaaat attccgggcg cagcagttcc
     6541 ttgatcttct tgtctggcgc ttttgcgact tcgttgagtc gtgaccgcaa gctggagagg
     6601 atatatttgc gcagtcgcca ggctttatcc gcatgcccgg attttcccaa agtgtctgca
     6661 taggtaagca gccaaaggta gtcttgggga tttgccttcg ttttacgctc cagccagggc
     6721 aacgcttcct tattctggcc aaccttggtc agagcaacac cataaacgcc ccataacgaa
     6781 gggtttttcg acgcttccga gcgccaggtc ttgatatgcg cttgcagcga ttccttgtcg
     6841 tccgatccga tcaggttcca caggattcct tccttggccg ttgcagatgc cggattgacc
     6901 ttcagggcct ggtgataatc tttcagtgcc gtttcaggct ccttctcgtt aagattaagc
     6961 tgagcgtgca ttaaccagta catctccgag tcccggaact gggactcgtt tttttctgcg
     7021 attttaaaaa gccgcttgag ctgttccgcc aaccccgcct gattcgcgac atccatcgaa
     7081 agcaatagcc agcggggctg gttaagccgt tgatagcctt cttcgccgac cgcaatggac
     7141 tcggtccact tgctcatgtc gcgagtaagt tgaatgagcc gttccgcgac gattgcgctc
     7201 gtgcttcccg atttccaaag gatgcggtaa gctcccaagg cgtgttcggg tcttttcatc
     7261 tcccacgaaa tatcccccag tacctcccaa aatggcaggt ctttctccgt taccttatcc
     7321 ttgttcgcca ccagcaccgc gagtgccttc tcggattggc catttttcca tatcagcctg
     7381 ccctgatggg ccagcgcctc tggcaagcga ttgaactccc caccgatatg ccgccatgta
     7441 acgatggctt cggcaagctg ccccgcgttc tcctgcgttt tagccagtgc ttcccacacc
     7501 tgcggattgt ccggatagcg atccagatag gatttcagga aattgacatg ctcggttaaa
     7561 ctgcccgctt cagcgtaagc ggacaacgca ctgttcattt catcgttggt gagcttccgc
     7621 ataccggata gctgtatcag catttccagt gcagtgccag tggaattcag tgcattcgaa
     7681 agcctgatgg tgttgatgat cgatggttcg tcttttctat ttctcgccag ccacaaccat
     7741 tccttcagcg cgacttcccg cattaaagcc cattcggcaa tacgccccag gttttcatgg
     7801 gcattttcat ccgcaggagc gagatcaacg atgcgttgcg ccaatgccac ggccgattga
     7861 agttctccta ccgcaagcgc acgatcagcc tgcttcctga tttgctccgt gttctcgggc
     7921 tcgagcgcca atctctgggc gccggtttca tacgctttcc tttgatcgtt acttgccagg
     7981 taaatgttga ttgcctcatc cagcaattcg ggatcttccg ggaacctctg ttgatacttg
     8041 ccgaggtatt gcaatgcgat ctggctttct ccagccgccc gtagcgcttc gagcgtcaga
     8101 aacgcaaact gtcgggcctg ctcgacatcc tcggataact cgtagcattc atgatatata
     8161 tcggccgcct ttctcggctg ctcggacgcc atatgccatc tagctgactc tttcagtttt
     8221 gccacgcgat cggaggaatc gtcagcactc tccgaccagc ggtaatataa atcggcaaca
     8281 acagaaggct gctcgagttc caggttgagt ttgataacct tcggcagaag agcaaccgga
     8341 acctgatttc tgctgatctc ggcaacgctg ttttgcagcg tggcaagctt ggttttccgg
     8401 cttgggttgt cttcccccgt agagtagtaa tccttgaagt caatttccaa tgccagcagc
     8461 ctcgtatcaa gctcgcccgg accattcctc gccagcagcg gttccagcgc agttcgcgcc
     8521 tcggccaact cgcccatgtt aatgtaatgc cgggcaagct gaatccgtaa accaacgtcc
     8581 tccggattca tgttcaatag aagctgtaaa tagagtactg agagatggtc cggcctctcc
     8641 acctgcaaca attcctttaa ccgatcctcc ttgtaaaaca aaagcgcaaa aacaaggatt
     8701 acccctaaac agaaaagaaa tatcgttttt tttaaactgc cgaacagatt catgattgga
     8761 ttctcttaag gtttcgtcta ttcatacatg agggcaatat cagttctgac cagccgagac
     8821 ccaaaccttc gttgcgcaga aagctttgat gcttaaaaat atgactttta tccagtcact
     8881 ttcctgataa cccaaccgtt tgttttggaa ggagcttgat acggcctccg gggtatgtcc
     8941 gttcggtcaa taacataaac agggtgattg ttacagcccg gatcaaaaaa ggcaaactga
     9001 aaagatatat gctcggacat cccgtacggt actcaaaagt ggaaggggat aaaacggggt
     9061 ttggaacaac gtcatgattt tccgcgtacc ttagtatctg agaaggacgt gcacctggaa
     9121 tgtctataga atgtctatcg aggtagattg ctcgatcata acgaggatgt gcggccagag
     9181 cagcaagaca agacccgcta ataacacacc agcgtcgcat ttcccgggtc ggccactgga
     9241 aaaataaatt tccagccttt attttcatgc tgcccgtcga gtcttccgcc agaccaattt
     9301 acataacatt tttgtccggg gggcccagtg accatttcca acggcacgtg agaacgcact
     9361 tgaaaattga ccccttccgc ggtctcctgc cattcctcta tctctccgtt gctgtgcaac
     9421 agatggatgg acgagggagg atcagttgac gtatatagca ggacgcggcc attcttggga
     9481 aaaagatgca cgtacctccc ttgatttaca tcatgaactc ccacaatgcc ctccgatctt
     9541 tgcaagtccg gccatcctgt tgaaacggga aggcgtaacg tacgcagttt gtcgacccca
     9601 cgaatatccc acgcgccgtc gaggcgacgg gacaaggtga tgttatagaa ttcatttact
     9661 ttctggctgt attcacttac ccagacaggc ctttgttcct gtttgacgct ccagtcataa
     9721 accgatttca gcccatcggt agaggttatt tttattcccg aatagaagtg atagtgaata
     9781 tgcataggtt ttagacgaag cgggttatcc gtaagctgca tcgtgtcgat aacccccgta
     9841 aacccgggct cttgtcgccg gtttatataa aacgcatcgc ttgcgatggg tgcatacacc
     9901 tggatctgac cgtcgatcga atagcccgta gggggaacgc gcgttacggt cttctcatcg
     9961 ctcgatatcg tagcgccacc cccattcatg ttctccaatc caagcgaatg gaccaagctg
    10021 agcgcatctt tgccagccaa tccatcccct gtccatagga aaacacgggt atccttgccc
    10081 tcaggggcaa gcgttttatt gatatattct actgatccgg cgatttcccg ctggatgtca
    10141 aacccatatc cgctaatggg taggcgataa tcatcgccaa ccgtagaaat attgttcttc
    10201 gcaaaccatg atgaaggatg actataagaa tggctggcaa tctccacatt gttcattttg
    10261 aaaacgtctc ttgctgtttt ctcggcctca ctcaatccgc ctgaccgttc gccgttcgct
    10321 atttcgccct cgattatcga aacagttgaa ggcagcgggt atttttgcag gaagcgatgc
    10381 tgaataacct tcatcgcagt tggcctaccg tcgatctccg ctttagcccc tgccccatca
    10441 ccgtcaattt gtacaagcag cagacgccgc ccattctcag tcgtaacatc tggaacgggt
    10501 attcccgaca gatcgagagc cgtggataaa aactcgaacg gattgatgat ccatcgttgg
    10561 cgcccctggt ggccgtgcgc taccacatag ggatgcaagg caacaccccc ccattgtccc
    10621 gaaaaaacag cgaccagcgg agtttccggc tgctctcccc agctcaaatg cttttcggtg
    10681 ctcccgtcga gtgttcgcca gagcggaaac ccgggtgtgc cgggcgccgg ttgcgcttca
    10741 tacccgatta tcctgtcgtt gaacaccatt gagccaggtt ctacagatga ttccgactga
    10801 ctcgactgga cccctagatg atgcaaaaaa gaattgtcag ctttgaaacc aagatttccc
    10861 agaacaacta cttttacccc tgattgcatc tgtcgcaata gccattcccg ataaacctcc
    10921 ggcctcaat
//
LOCUS       DASSHJ010000127         6583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1091771,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000127 DASSHJ010000000
VERSION     DASSHJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091771"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="dapE"
                     /locus_tag="VFI43_05045"
     CDS             <1..817
                     /gene="dapE"
                     /locus_tag="VFI43_05045"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET7061527.1"
                     /translation="AIEKFITDHPDHGGSIALLVTSDEEGIAVDGTVRVVEALKARNE
                     SIDYCIVGEPTCVDKLGDTIKNGRRGSLSGTLIVKGIQGHIAYPHLAKNPIHLVAPAI
                     AELACTEWDNGNEYFPPTTWQISNINGGTGAANVIPGEVTLLFNFRFSTASTVESLKA
                     RFQEILDRHHLDYDLHWEFSGKPYLTPRGTLVEAISAAIKTVTGIEPQLSTSGGTSDG
                     RFIADICPQVVEMGPRNATIHKINEYVELKDMAQLPEIYRLTLERLLLPAAST"
     gene            900..1835
                     /gene="prmB"
                     /locus_tag="VFI43_05050"
     CDS             900..1835
                     /gene="prmB"
                     /locus_tag="VFI43_05050"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7061528.1"
                     /translation="MYDEARAQLYTLRDVLRFAVSRFNEAGLFFGHGSASAYDEAAYL
                     ILHVLHLPLDRLEPFLDAGLTVSEREQVLDVIRRRATEKIPAAYITREAWLGDFSFYV
                     DERVIVPRSFIAELLRERLSPWIEQPDHIQSALDLCTGSGCLAILLGHAFENAQIDAV
                     DISLDALEVAQKNVADYHLQDRISLIQSDLFAALSGRRYDLIVSNPPYVSAACMASLP
                     AEYQQEPHEALAGGTDGLDAVRRIIQEAARLLTDQGLLIVEIGHNREILEEVFPNIPF
                     TWLETSGGDGFVFLLARDQLPNPVQNSLPRLPFTA"
     gene            complement(1846..2589)
                     /locus_tag="VFI43_05055"
     CDS             complement(1846..2589)
                     /locus_tag="VFI43_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7061529.1"
                     /translation="MQKLLAHAGLGSRREMEELIASGRATINGKVANIGDRVGRDDIV
                     RVGKRIIHAKPAGRLPRVLLYHKPEGEIVSRDDPEGRASVFDKLPQLRSSKWISIGRL
                     DYNTSGLLIFTTDGELANRLMHPRFEVEREYAVRIVGRLTDEHVKTLTSGIELEDGLA
                     KFDRLSDEGGEGSNRWYRVTLKEGRNREVRRLFEALGLTVSRLMRVRFGPINLPPRLK
                     RGKSMELDEAETRRLLELMASSPTRINLG"
     gene            complement(2745..3341)
                     /gene="recR"
                     /locus_tag="VFI43_05060"
     CDS             complement(2745..3341)
                     /gene="recR"
                     /locus_tag="VFI43_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7061530.1"
                     /translation="MKISRSLDDLMNALRCLPGVGPRSAQRMAYHLLQRDHEGARHLA
                     DSLDYALQHIRHCEKCNNFTEETVCELCRSHQRDASLLCVVEMPADLMMIEQALCYKG
                     LYFVLMGRLSPLDGIGPRDIHLDRLLKRAQDGLVKEVVLATNFTVEGEATAHYVGEML
                     HGKGLKITRIARGLPVGGELEHVDSGTLAQALIERRIV"
     gene            complement(3594..3917)
                     /locus_tag="VFI43_05065"
     CDS             complement(3594..3917)
                     /locus_tag="VFI43_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7061531.1"
                     /translation="MKGGLGNLMKQAQQMQENMKAMQEKLASIEVEGQSGAGMVKVVM
                     TCRYDVRRVSIDSALMGDDKEMLEDLIAAAVNDAVRRVESTTQEKMAGFTSGLNLPPG
                     MKLPF"
     gene            complement(3933..5648)
                     /gene="dnaX"
                     /locus_tag="VFI43_05070"
     CDS             complement(3933..5648)
                     /gene="dnaX"
                     /locus_tag="VFI43_05070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7061532.1"
                     /translation="MPQTQVLARKWRPKRFIDLTGQDHVVRALTNALEQKRLHHAYLF
                     TGTRGVGKTTIARILAKAFNCEAGVSSNPCGACSACTEIDNGRFVDLIELDAASNTQV
                     DKMRELLENALYAPTSARYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDP
                     QKIPVTVLSRCLQFNLKQIPVQLIAGHLKHVLEQEQISWDGGSVQLLARSAQGSMRDA
                     LSMLDQAIAFGEGRVEEAGVRSMLGAIDQDYLYDLMDALSQKNGVRMLEIADAMESRS
                     LSLDTALQELAVLLHRIALAQLVPSAIAEELPERAQLLDLAKGFSPEDVQLFYQIAIS
                     GREDLSRAPDEYAGFTMTLMRMLAFAPENPSMPGTITPANNTVERSSSFPGSAPLKKD
                     AARTRPENISTGKNKSSEAPVATKIRTNHVTELAAFKEKTQTTEASPAETETLSSDRW
                     LTITSQLKLSGMPKMLAQHCEVKRFSPEEIELRLPEMHRHLLDRSYQGRLQAALQEHL
                     GKPIRLKVSVGASTGLSPAERENREKQERQLQAVAAIESDPFVRELIENFDAKLIVSS
                     IKPNQ"
     gene            complement(5790..5888)
                     /gene="ffs"
                     /locus_tag="VFI43_05075"
     ncRNA           complement(5790..5888)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFI43_05075"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6187..6263)
                     /locus_tag="VFI43_05080"
     tRNA            complement(6187..6263)
                     /locus_tag="VFI43_05080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6227..6229),aa:Met,seq:cat)
     gene            complement(6288..>6583)
                     /locus_tag="VFI43_05085"
     CDS             complement(6288..>6583)
                     /locus_tag="VFI43_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061533.1"
                     /translation="QGKISQNCKTASRVKSPHRIMHYENHVWKYTRLSATAYSAFCPD
                     SALCANSHNDMPVVYKEDAIIAKKIMVGIRKQDDCATPDKAVDRLCGQIDLPH"
BASE COUNT         1495 a         1838 c         1716 g         1534 t
ORIGIN      
        1 tgcaatcgaa aagttcatca ctgatcatcc tgatcatgga ggttccatcg ccttgctcgt
       61 gacttctgat gaagaaggca ttgcggtgga tggaacggtg cgagtggtgg aggcgctcaa
      121 ggcgcgaaac gagtcgatcg attattgcat cgtgggtgaa cctacctgcg tggacaagct
      181 gggcgatacc atcaagaacg ggcgccgcgg ctccctgtcc ggcacgctga tcgttaaagg
      241 catacaaggc catattgcct atccccatct cgccaaaaac cccattcacc tcgtggctcc
      301 cgccatcgcg gaactcgcgt gtaccgagtg ggacaacggc aacgaatact tcccgcccac
      361 gacctggcaa atctccaata tcaatggagg cacaggggct gctaacgtga ttccgggcga
      421 agtcaccctc ttgttcaatt tccgtttctc gacggcaagc acggttgagt cgctgaaagc
      481 ccggttccag gaaattctgg acaggcatca tcttgattat gatctgcact gggaattctc
      541 gggcaaaccc tacctcacgc caagaggtac tttggtggag gcgataagcg cggcaatcaa
      601 aacagtaacc gggattgagc cgcaactttc cacgtcgggt ggcacttcgg acggccggtt
      661 catcgcggac atttgtcctc aagtggtgga gatgggcccg cggaacgcca cgattcataa
      721 gatcaatgag tacgtggagc ttaaggatat ggcgcagtta cccgaaattt accgcctgac
      781 gctggagcgt ttgctgctgc cagcagcatc cacctgaact tcaatcaggc ggtgtatgca
      841 tccccgtcat tgcgtcgtca gccacctgaa tttcaccccc tttcccggca taggcgccta
      901 tgtatgacga ggctcgggca caactctata ccttacggga tgtgcttcgc tttgcggtaa
      961 gccggtttaa cgaagccggc ctgttttttg gtcacggctc ggcttcagca tatgatgaag
     1021 ctgcctatct cattcttcat gtgctgcacc tcccgctcga tcggctagag ccctttcttg
     1081 acgccggtct taccgtatct gaacgggagc aggtgctgga cgtgatccgg cgtcgggcaa
     1141 cagagaaaat tcccgctgct tatatcaccc gggaagcgtg gctgggggat ttcagttttt
     1201 acgtggatga gcgggtcatc gtgccgcgct ccttcattgc cgagttgctg cgcgagcgtc
     1261 tgtcaccctg gatagaacag cccgatcata tccaatcggc gctcgacctt tgcactggct
     1321 cggggtgcct cgcaatactg cttggccatg ctttcgagaa tgcgcaaata gatgccgtgg
     1381 atatctcgct tgatgcgctt gaagtcgctc agaagaacgt ggcggattac catttacagg
     1441 ataggatcag cctcattcag tccgatctgt ttgccgcgtt gagcggacgc cgctacgacc
     1501 tgattgtcag caatccaccc tatgtcagcg cagcatgtat ggcatccctc cctgcggaat
     1561 accagcagga accacatgaa gccttggcag gagggacgga cggtctggac gcagtccgaa
     1621 ggataattca ggaggcagct cgtcttttga ccgatcaggg actgctgatc gtggagattg
     1681 gccacaatcg cgagatactg gaagaagttt ttccgaacat tccctttacc tggctcgaaa
     1741 ccagcggcgg agacgggttc gtttttctgc ttgcccgaga tcagcttccc aaccctgtcc
     1801 agaactctct accgagactc cccttcactg cttgatggaa aagcgttagc caaggtttat
     1861 gcgggtaggg gaagacgcca tcaactccag taaacgcctc gtctctgcct cgtccagttc
     1921 catcgatttt ccccgtttca accgaggcgg caaatttatc gggccaaaac gcacccgcat
     1981 gagccggctt acggtcagcc ccaatgcctc gaacaagcgg cgcacttcgc ggttccgccc
     2041 ctccttcagc gtcacccggt accagcggtt ggacccttcg ccgccttcat ccgacaatcg
     2101 gtcgaatttt gcaaggccat cttccagttc gatgccggat gtaagcgttt tcacatgctc
     2161 gtcagtcaaa cgaccgacaa ttcttaccgc atattcacgc tcgacttcaa accgagggtg
     2221 cattaagcgg ttagccagtt cgccgtccgt ggtaaaaatc aacaggccac tggtattgta
     2281 atcaagcctg ccgatcgaaa tccatttcga ggagcgcaac tgggggagct tatcaaacac
     2341 cgaggcacgc ccctccggat cgtcgcggct gacaatttcc ccttcaggct tgtggtaaag
     2401 cagtacgcgc ggcaagcgcc ctgctggctt ggcgtggatg atccgcttgc caacgcgcac
     2461 aatatcgtca cgtcctaccc ggtcgccgat attagccacc tttccattga tagtcgcacg
     2521 accggacgct atcaactcct ccatttccct gcgtgaaccc agtccggcat gcgccaggag
     2581 tttctgcaat ttttgcgtct ctcctgaggg catcgccgcg tttaacttcg cagaattcct
     2641 ggagggagtt cttttagttt ttggattcaa ggattgagga aattggaaaa taacggatcg
     2701 aagaattgag gcgatatcaa tggcagagga tggcggaatt gacgctaaac gatacgacgc
     2761 tcaatgaggg cctgagcgag cgtaccgcta tccacgtgtt caagctcccc gcccacgggc
     2821 aagccacgcg cgatgcgcgt gatcttcaag ccctttccgt gcagcatttc gccaacgtaa
     2881 tgtgccgtgg cctcgccttc caccgtaaaa ttcgtagcga gaacaacttc ttttaccaac
     2941 ccatcctgag ccctctttag caagcgatca agatggatat cacgtgggcc gatcccgtcg
     3001 aggggggaaa gcctccccat cagcacgaaa tacagcccct tgtaacagag ggcctgctcg
     3061 atcatcatca gatccgcagg catctcgacc acacaaagca atgaagcatc tcgctgatgg
     3121 gacctgcata gctcgcaaac ggtttcttca gtgaagttat tgcatttctc gcaatgcctg
     3181 atgtgctgca gcgcatagtc taacgaatcc gcaagatgcc gggcgccctc gtgatccctc
     3241 tgcagaagat ggtaagccat gcgctgcgcc gaccttgggc cgacgccggg taggcagcgt
     3301 aatgcgttca tcaggtcgtc gagactgcgg gagatcttca tgaggcgggg aaaaatagac
     3361 aaatgagggt aggcaaatga agcatcccag gccaagtttc cgggctgtac tgctgagttt
     3421 gaaccaggtt tgcgtcaggc ttcttgcaga tatcaaaacc atatttgaga aaaatggaac
     3481 catgcttcgt cctcgaactt gactaaagcc gattataaaa gtactcgtat gcacagccat
     3541 gaaaaggcgc gtgctaggga ttagtcccga gaacgagcgt ggttatgagc gtgctaaaag
     3601 gggagtttca tgccaggcgg caaattcagc cctgaggtga accccgccat cttttcctgc
     3661 gtcgtggact ccacccgccg caccgcgtcg tttaccgccg cggcaatcag atcttccagc
     3721 atttccttat catcacccat aagggcacta tcgatactca cgcgcctgac atcataacga
     3781 caggtcatga ccaccttgac cataccggcg ccggactgac cttcgacctc aatcgaagcc
     3841 agtttctcct gcatggcttt catgttttcc tgcatctgct gagcttgttt cattagattg
     3901 cccaggccac ctttcatcat tttgcacctc cgctattgat tgggcttgat cgacgagaca
     3961 atcagttttg catcgaagtt ttctatcagt tcccgcacga agggatcaga ctcgattgcc
     4021 gcgacagctt gtaattgcct ctcctgtttt tcacgattct ccctctctgc cgggcttagg
     4081 cctgtgcttg ccccgacaga caccttcaag cggatcggct tgccaaggtg ctcttgaaga
     4141 gccgcctgca gtctcccctg ataagatcta tccagcagat gtcgatgcat ttccggcaat
     4201 cgtaattcta tttcttccgg cgaaaagcgc tttacctcgc agtgctgcgc tagcatttta
     4261 ggcattccac tcaatttcaa ctgactcgtg atcgtcaacc agcgatcgct gctcaacgtc
     4321 tcggtctcgg ctggacttgc ttccgttgtc tgcgtttttt ccttgaaggc ggcaagctcc
     4381 gtgacatgat tcgtcctgat cttcgttgcg actggagctt ccgatgattt atttttccct
     4441 gttgagatat tttcagggcg tgttctcgca gcatcctttt tcaaaggcgc gcttcctggg
     4501 aaggaggatg agcgctctac cgtattattg gcaggagtta ttgttccagg catggatggg
     4561 ttttcgggcg cgaaagccag catgcgcata agtgtcatgg tgaaacctgc atattcatcc
     4621 ggcgcccggc tcaagtcttc gcgcccactg attgcaatct gataaaacag ttgcacatct
     4681 tctggagaga accctttggc gagatccaga agttgcgccc gctcggggag ttcctctgca
     4741 atcgcggacg gcaccagctg tgccagggcg atacgatgca gcaagacagc aagttcctgc
     4801 aaagcagtgt ccagagacag gctacggctt tccatggcgt ccgctatttc aagcatccgc
     4861 accccgtttt tctgcgacag cgcatccatg agatcgtaga gataatcctg atcgatcgcg
     4921 ccgagcatgc tccgcacgcc ggcctcttcc accctgccct cgccaaaagc gattgcctga
     4981 tcgagcatac tgagggcatc acgcatgctt ccctgcgcag accgcgccag caactgtacg
     5041 gacccgccat cccagctgat ctgctcctgc tcaagcacat gcttgaggtg ccccgcaatg
     5101 agttgcacgg ggatctgctt caggttgaat tgcaagcacc gcgacagcac cgttaccggg
     5161 attttttggg ggtccgttgt cgcgagaata aacttgacgt gctccggcgg ctcttccaga
     5221 gttttcagca tcgcattgaa tgctgatttg gaaagcatat ggacttcatc aatgatatac
     5281 accttgtagc gggctgaggt aggcgcgtaa agcgcgtttt cgagaagctc ccgcatcttg
     5341 tccacttggg tattcgaagc agcatcgagc tcaatcagat cgacaaaacg gccgttgtcg
     5401 atttcagtgc aggcagaaca cgctccgcaa ggattcgaac ttacgcctgc ctcgcaattg
     5461 aacgctttag cgaggattcg cgcaatagtc gtcttgccca cgccgcgggt gccagtaaac
     5521 agataggcgt gatggagcct tttttgctcg agcgcgttgg tcaacgcacg gaccacgtga
     5581 tcttgtcccg tcagatcaat gaagcgcttt gggcgccatt tgcgggcgag aacttgagtc
     5641 tgcggcacga tatccagaaa gtaaacggtg attgccagga cggagtgaat ggccgaaaat
     5701 cgacaacagc gattgcttca gccatcttca cgcggggaag catcgaggcg aatcgagccg
     5761 attgcaccga gcagccccgt gcgcatttgg gtggcgagcc cgatccccgg cacttgcaag
     5821 aaatagctgt ggctgcttcc ttccggacct gaccaggttc actaccgtgc aatgcgggga
     5881 ggcccgccgt aaaagctgaa acaatgaaaa cgttaattag aactgcgtta ttgcatcggc
     5941 actattttgc acggagtcga agtccgcgtc tacatgaatg aagccggcaa atcatgggaa
     6001 gttacacgag tcgcgtcagg agcgggagca ggaacaaaat ggaaactcgc gagcaacgtt
     6061 gggcttcagc taaaccgcgc attggaagga accgatagca gggaacgtat ggacggacaa
     6121 accacacgac aaaccagaca aacttccccg ggaatccctc gcaaatgagg aaacgagggg
     6181 aaagagtggt tgcgggggca ggatttgaac ctacgacctt cgggttatga gcccgacgag
     6241 ctgccagact gctccacccc gcgccagagt ggataataat agcaaaatta atggggaaga
     6301 tcaatctgtc cacacagccg gtccaccgct ttatcaggtg tcgcacaatc atcctgcttt
     6361 cttattccga ccattatttt tttagcaata atggcgtctt ctttataaac aacaggcata
     6421 tcgttatgcg agttagcgca cagagcagaa tccggacaaa aggcactata agctgttgca
     6481 gataatcgag tatatttcca tacatgattt tcataatgca taatacgatg aggtgacttg
     6541 acgcgcgaag ccgtcttgca attctggctg atcttccctt gct
//
LOCUS       DASSHJ010000128         9309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_619665, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000128 DASSHJ010000000
VERSION     DASSHJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619665"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFI43_05090"
     CDS             complement(<1..240)
                     /locus_tag="VFI43_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004731677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7061534.1"
                     /translation="MSIEVRNLSKQFGTFSALRNVSLEVTSGELLALLGPSGSGKTTL
                     LRVIAGLEAADTGQVLFQGEDATDQHVRERQVGFVF"
     gene            complement(245..1147)
                     /gene="cysW"
                     /locus_tag="VFI43_05095"
     CDS             complement(245..1147)
                     /gene="cysW"
                     /locus_tag="VFI43_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HET7061535.1"
                     /translation="MSTVNSLAPSFAVPLPSSVRRPRATHEPAWVRWTLIGVALAFLT
                     LFLFIPLISVFYEALKKGTDVYIAAITDPDAVSAIQLTLTAAAIAVPLNLIFGLAAAW
                     AIAKFEFRGKNLLITLIDLPFSVSPVVSGLIYVLVFGLQGWLGPWLAEHDVKMIFAVP
                     GIVLATIFVTVPFIARELIPLMQAQGTEEEEAAIVLGANGWQTFFHVTLPNVKWGLLY
                     GVILCNARAMGEFGAVSVVSGHIRGSTNTLPLHVEILYNEYNFAAAFAVASLLAMLAL
                     VTLVLKTLVESKNNGQKESKEQGE"
     gene            complement(1158..1991)
                     /gene="cysT"
                     /locus_tag="VFI43_05100"
     CDS             complement(1158..1991)
                     /gene="cysT"
                     /locus_tag="VFI43_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET7061536.1"
                     /translation="MSTFKQHSVLPGFNLALGFTLLYLSLIVLIPLSAAFIRTASLSW
                     EEFWAVVTTPRVLASYRLTFGASFAAALVNAVFGLLVAWVLVRYRFPGKRLVDALVDL
                     PFALPTAVAGIALTALYAGNGWIGQFFEPLGIKIAFTPLGVFVALTFIGLPFVVRTVQ
                     PVLEDIEAELEEAAATLGANRFQTFSRIIFPTLFPALITGFALAFARAIGEYGSVIFI
                     AGNMPMISEITPLLIITKLEQYDYAGATALAVVMLSISFIMLLIINLLQWWSRRRGPF
                     A"
     gene            complement(2015..2884)
                     /locus_tag="VFI43_05105"
     CDS             complement(2015..2884)
                     /locus_tag="VFI43_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7061537.1"
                     /translation="MSMSRMPRARKDVVERIQEATDYPVRRLEDGWVAGHFFNCWLSS
                     VFQPIADMSARKVMGHAAYIRSATDGTTVLSPWKLFALTEGDTLLVRFDRLCRAIHAI
                     NYFEHAPQGNLFVTVQPRLLESVKDDHGRAFESILNIIGIETSRVVIEIPIEVNRDSR
                     LLKHVISNYRSRGYQIAVNHSSTNEDWLAELASLYPLYPDIVRLEASVLLRHSGTGSL
                     VDTIHHFGASVLVHEIETSQQMAIAAQAGADFLQGRALGVPARAIESVVPIIEGPYTT
                     FGAVPGAGRALKV"
     gene            complement(2957..3967)
                     /locus_tag="VFI43_05110"
     CDS             complement(2957..3967)
                     /locus_tag="VFI43_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7061538.1"
                     /translation="MNLKARLTSTLLPSLVIASLVLSGNAWAEKAILNVSYDPTRELY
                     QQFNSAFAKYWEQKANEKVTIKQSHGGSGKQARSVIDGLEADVVTLALANDINAISEK
                     AKLLPGDWQSRLPQNSTPYTSTIIFLVRKGNPKGIKDWNDLARPGVSVITPNPKTSGG
                     AQWNYLAAWEFGKRHFGESRAKDFVADIYRNVPVLDSGARASTTTFVERGVGDVFISW
                     ENEAFLAIKELGAGKFEIVVPSLSILAEPPVAVVDKVVDKKGTRAVAEAYLQYLYSDE
                     GQEIAAKNFYRPTNPKVAEKYAAKFPSIKLFKIDEAFGGWKNAFKVHFADGGTFDQIY
                     QR"
     gene            complement(4020..5738)
                     /locus_tag="VFI43_05115"
     CDS             complement(4020..5738)
                     /locus_tag="VFI43_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HET7061539.1"
                     /translation="MKFFWRIAFAFAIGTLTIKPLQADEKSNKQVDSTNLQGMRQMAQ
                     AASSGSELSGSALSDSDKSPSRSSGEDFKWEIGPSNENYKWAIPALDKSAASDKPAST
                     SSAKPAAAKKSTSYFKPSSSYGTQPDSDPPRYVRNLSKIGIEAFKDITWLDIGFEHRT
                     RYEFRSNDIRRTQFLIDEPFLLRSRGYLGIKEILDPLRGAVEFQDSRRYNGHFPRDDR
                     DWDQYDLIQAYGELYFKGALGEDPRKQQRPIRFRAGRMAYETLDRRLIARNEWRNTTN
                     AFEGFRVTLGQEANNWEIDTWAYQPVKRLIDDFDQRTKNVWFYGVIGHWREWSDIITL
                     QPYYMGLVQDGSKVPGQIDREIHSPAIRGYGKIPNTNLDFDFNLIYQFGRNAGQRHEA
                     HGYTLEVGYTFEHSWKPRLMAFYGYATGDGNPNDNVDNRFERFFGFARPWSADDYIVF
                     ENIKAPKIRLEFQPAKDLRIDGGYSWYWLASSTDRFNNLLDGNNSAIRNPGFNRDATG
                     QSGDFIGHNFDIRARYKLTANIDTTIGYSHFTSGEFTRNRQIAALGKSPGSSDFFYVE
                     VVLTAF"
     gene            complement(5910..6122)
                     /locus_tag="VFI43_05120"
     CDS             complement(5910..6122)
                     /locus_tag="VFI43_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HET7061540.1"
                     /translation="MMGNTTITDLEERNSSSTHPGTRIGKRIPPHIKEQILSTIANIE
                     YGSVEVVVHDGKVVQIECREKLRVGR"
     gene            6377..7276
                     /gene="ttcA"
                     /locus_tag="VFI43_05125"
     CDS             6377..7276
                     /gene="ttcA"
                     /locus_tag="VFI43_05125"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET7061541.1"
                     /translation="MIGAPSRKEQHEVNKLRKRLRRLVGTAIADFNMIEEGDRVMVCL
                     SGGKDSYALLDILRSLQAHAQTRFELIAVNLDQKQPGFPERVLPEYLSAIGMPFRIVE
                     QDTYSVVKRLIPEGKTTCSLCSRLRRGVLYRVAGELGATKIALGHHRDDILETLFLNL
                     FYGGKLKTMPPKLVSDDGRNIVIRPLAYCKEKDLAAYAEMENFPIIPCNLCGSQKNLQ
                     RLVVGEMLEHWDKKYPGRLETMFTALQNIQPSHLLDPALYDFKGLKTGNEPFPEGDRA
                     FDPETFEPAMEEIISLSKGLEGT"
     gene            7316..8680
                     /locus_tag="VFI43_05130"
     CDS             7316..8680
                     /locus_tag="VFI43_05130"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET7061542.1"
                     /translation="MTRYGFAWLPAILIVGMVLLPSAHSSVPVMDGKNGLPTLAPLLQ
                     NVTPAVVNISVKTRSALEENPLFRDPFFRRFFNIPDQAERVEQSAGSGVIVDAANGYV
                     VTNFHVIKDALQVLVTLKDRRQFQAKLVGTDPGTDIALLKIDAKKLQALRLGNSDLLN
                     VGDFVVAIGNPFGLGQTVTSGIVSALGRSGLDIEGYEDFIQTDASINPGNSGGALINL
                     KGELIGINTAIIGPSGANVGIGFAVPSVMVKAVVDQIARYGEVRRGRLGASSEDITYD
                     LAGSLGLASTDGAIISSVEPDSPAATAGLKARDVVTGVNGRSVRNSIDLRNKIGLMPV
                     GEILNLALIRSGKPLTVKVKIGKPLDVASTEAETVPQLAGATVANFKSGSPRGAEGVV
                     VTKVEPNTPAWMHGLRPGDIIVGVNRKKVRSVTEFLTAVQASRDSVYLNLVRGDFRVT
                     LMIR"
     gene            8885..>9309
                     /locus_tag="VFI43_05135"
     CDS             8885..>9309
                     /locus_tag="VFI43_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7061543.1"
                     /translation="MQERYRPGEVESEAQRHWEQSKAFKAVETPGKQKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRYWRMQGYNVLQPMGWDAFGLPAENAAMQNHVSPALWTY
                     DNIAYMRNQLQSLGLAVDWSRELATCQPDYYRWNQWFFL"
BASE COUNT         2075 a         2528 c         2552 g         2154 t
ORIGIN      
        1 gaagacaaat cctacctgcc gttcgcgcac atgctggtcc gttgcgtctt ctccctggaa
       61 cagaacctga ccggtatctg cggcttccag ccctgcgatc actcgcagca acgtcgtctt
      121 gcccgacccc gaagggccga gcagcgccag caattcaccc gaggttactt ccaggctgac
      181 gttgcgaagt gctgaaaagg taccaaactg tttcgataga ttacgaacct caatactcat
      241 tgttttattc cccttgttcc tttgattcct tctgtccgtt attttttgat tcaaccagcg
      301 ttttcagcac gagggttacc agggccagca tggcgagcaa cgaggccacc gcaaacgcgg
      361 cggcgaagtt gtattcgttg tagagaatct cgacgtgaag cggcaacgtg ttggtactgc
      421 cgcgtatatg cccggatacg actgagactg ccccaaattc tcccattgcg cgtgcattgc
      481 agagaatgac cccatagagc aacccccatt tgacattcgg cagcgtcacg tgaaaaaaag
      541 tctgccagcc gttcgcaccc agcacgatcg cagcttcctc ctcctcggtt ccttgcgcct
      601 gcatcagcgg tatcagctcg cgcgcaataa agggaacggt cacgaatatg gtggcgagca
      661 cgatcccggg cacggcgaag atcattttga cgtcgtgctc ggccagccag ggccccagcc
      721 agccctgcaa gccgaaaaca agcacgtaga tcagccccga gaccaccgga gaaaccgaaa
      781 acggcaaatc gatgagcgtg atcagcaggt tcttgcctcg gaattcaaat ttggcaatag
      841 cccaggcagc ggcgagtccg aatatcaaat tgagcggcac cgcaatagcc gcggcggtca
      901 gggtaagctg tatcgccgat accgcgtcgg gatcggtaat cgcagcgata tacacatcgg
      961 tgcccttctt gagcgcttca tagaacaccg aaatcagggg aatgaaaaga aacagcgtta
     1021 gaaacgccag cgcaaccccg atcaatgtcc agcggaccca cgcaggctcg tgggttgccc
     1081 gagggcgacg cacgcttgaa ggcaagggaa cggcaaaact gggggctaaa ctgtttacag
     1141 tactcatcgt ggttgtctca tgcgaaaggg ccgcggcgcc ggctccacca ttgcagcagg
     1201 ttgatgatca gcagcatgat gaaagaaatg ctcagcatca cgacggcgag cgcggtcgca
     1261 ccggcgtaat cgtactgctc aagcttcgtg atgatcagaa gcggtgtgat ctcggagatc
     1321 atcggcatgt tgccggcgat gaaaatcact gagccgtatt ctccgatcgc tcgcgcaaag
     1381 gcaagcgcaa agcctgtaat cagcgccgga aaaagggtag ggaaaataat tcgggaaaat
     1441 gtctgaaagc ggtttgcgcc aagcgtggcc gctgcttcct ccagttccgc ctcgatgtct
     1501 tcgagcactg gttgcacggt acgcactacg aaaggcaagc cgataaaggt caatgccaca
     1561 aaaacaccaa gcggcgtgaa agcgatcttg atgccgagcg gctcgaaaaa ttgaccgatc
     1621 cagccgtttc ctgcatacag cgcagtcagg gcgatgcctg ccaccgcggt gggcagcgca
     1681 aatggaagat cgaccagggc atccaccagc ctttttccgg gaaaacgata acgtaccagc
     1741 acccaggcga cgaggagccc gaacacggca ttgacaagcg ctgcggcgaa ggaagcaccg
     1801 aatgtcagcc gatacgatgc aagtactcgg ggagtcgtca cgacggccca gaactcctcc
     1861 catgacaggg aggcagtccg gataaaggca gccgacagtg gaatcaacac gatcaggctt
     1921 agatacagca gcgtgaagcc aagcgcaaga ttgaatcctg gcagcacgct gtgctgtttg
     1981 aatgtgctca cgacaattcc tctctttcgg attttcagac tttcaatgcg cggcctgcac
     2041 cggggactgc gccgaacgtg gtgtaaggac cttctatgat cggcacgact gactcaatcg
     2101 cccgggctgg cacgcccaat gcccggccct gcagaaaatc agctcctgct tgggcggcaa
     2161 tggccatctg ctgcgatgtt tcgatctcgt gcacgagaac cgaggcgccg aagtggtgga
     2221 tggtatccac taacgagccg gttcccgaat ggcgcagaag aacggaagct tccagcctga
     2281 caatatcggg gtacagtgga tacagactgg cgagctccgc cagccaatcc tcatttgtac
     2341 tactgtggtt aacggcgatc tgatagccgc gagagcgata attgctgatc acatgcttaa
     2401 gcagcctcga gtcgcgattg acctcgattg gaatttcgat aaccacgcgc gaagtttcga
     2461 tgccgatgat attcaggatg ctttcgaacg cccggccgtg gtcatccttg acgctttcca
     2521 gcaggcgtgg ctgaaccgtt acaaaaagat ttccttgcgg agcatgctcg aaatagttga
     2581 tggcatgaat cgcgcggcat aagcggtcga aacgcaccag caatgtatcg ccctcggtta
     2641 gagcaaagag cttccacgga gacaggacgg tcgtgccatc agtggctgag cgaatataag
     2701 cagcgtgtcc cattaccttg cgtgcactca tatccgcaat gggctggaag acgctcgaca
     2761 accagcagtt aaaaaaatgt ccagctaccc atccgtcttc caggcgccgt acggggtagt
     2821 ccgtggcctc ctgaattcgt tccactacat cttttctcgc tcttggcatc ctgctcatgc
     2881 tcatgctcct ttgatggggt tgcttgccgc ttgtattacc agtagatgcg atggttacga
     2941 tgcggtcctt gccttgtcat ctctgataga tctggtcgaa agtgccgccg tccgcaaagt
     3001 gaaccttgaa tgcattcttc catccgccaa aggcttcgtc gatcttgaac agtttgatgg
     3061 agggaaactt ggctgcgtat ttttcggcaa ctttcggatt ggtgggacgg tagaaattct
     3121 ttgcagcaat ctcctggcct tcgtccgaat aaaggtactg cagataagcc tcggcgaccg
     3181 cgcgggtgcc cttcttgtcc accaccttgt cgaccacggc gaccggcggt tctgcgagga
     3241 tgcttagcga cgggactaca atctcaaact tgccggcgcc cagttccttg atcgccagga
     3301 aagcttcgtt ttcccaggat atgaagacgt ctcctacccc acgctcgacg aacgtcgtcg
     3361 tcgaggcacg cgcgccggaa tcgaggaccg gaacgttcct gtagatatcg gcaacaaaat
     3421 cctttgctct tgactcccca aaatgacgct ttccgaattc ccaggccgcc aggtaattcc
     3481 attgggctcc acctgaggtt ttcgggttgg gcgtgatgac cgatacgccg ggccgtgcca
     3541 gatcgttcca atccttgatg cctttaggat tgcctttgcg caccaggaaa ataattgtcg
     3601 aagtgtaagg ggtgctattc tggggcaggc gtgactgcca atcgcctgga agcaatttcg
     3661 ctttctcgga gatcgcgttg atgtcgttag ccaacgcaag tgtcaccaca tcagcctcca
     3721 gtccatcgat caccgatcgg gcctgcttgc ctgagccgcc atgagattgc ttgatggtca
     3781 ctttctcgtt ggccttctgt tcccaatatt ttgcgaacgc gctattgaac tgttgataaa
     3841 gctcccgagt ggggtcgtag gagacattca gtattgcctt ctcggcccat gcgtttccgc
     3901 tgagcacgag acttgcgatt accaggctgg gcaacagggt ggacgtgagc cgggctttga
     3961 gattcatcat ttttcctttt atgaattggt taactatgga tggatagagt cggcgaacat
     4021 caaaaggcgg tgaggacgac ttcaacataa aagaagtccg aactccccgg gcttttgccc
     4081 aaggcggcaa tttgccggtt cctcgtgaac tcgccgctcg taaagtgcga gtagccgata
     4141 gtcgtatcaa tgttcgccgt aagcttgtag cgggcacgga tatcgaagtt atgcccgatg
     4201 aaatcgccgc tctgccctgt cgcgtcgcgg ttgaaccccg ggttccttat ggcgctgtta
     4261 ttgccatcca ggaggttatt gaaccggtcc gtactgcttg caagccagta ccagctatag
     4321 cctccgtcta tccgcaggtc tttggcgggt tgaaattcca gtcttatttt tggcgccttg
     4381 atattctcaa agacaatgta gtcgtcggct gaccagggcc gggcgaagcc gaagaagcgc
     4441 tcaaagcggt tgtcgacatt gtcattgggg ttgccgtcgc ccgtcgcgta accataaaac
     4501 gccatcaggc gcggcttcca gctatgctcg aaggtatacc cgacttcaag cgtgtaaccg
     4561 tgggcctcat ggcgctgtcc ggcattccgc ccaaactggt agatcaggtt gaagtcgaag
     4621 tccaggttgg tgttgggaat ttttccgtag ccgcgtattg caggtgaatg aatttctcgg
     4681 tctatttgcc cgggtacctt gctgccatcc tggacaagcc ccatgtagta aggctgcaat
     4741 gtgatgatgt cggaccattc tcgccagtga ccgattaccc cgtagaacca aacgtttttg
     4801 gtgcgctgat cgaaatcgtc tatcaggcgt tttaccggct gataggccca cgtatcgatc
     4861 tcccagttgt tggcttcctg gcccagggtg acacggaagc cttcaaatgc attggtggta
     4921 ttacgccact cgttgcgcgc gatgagacgt cgatccaatg tttcgtaggc catccgcccg
     4981 gctcggaacc ggatgggccg ttgctgcttg cgagggtctt cgcccaaagc gcccttgaag
     5041 tagagctccc catatgcctg gatcagatca tattgatccc agtcgcggtc atcgcggggg
     5101 aaatgtccgt tatagcggcg cgagtcctgg aattcgaccg caccccgcag cggatcaaga
     5161 atctccttga tgcccaggta gccgcggctc ctgagcagga agggttcgtc gatcaggaat
     5221 tgtgtccgtc ggatatcgtt gctgcgaaat tcgtaccgcg tacgatgttc gaagccaatg
     5281 tccagccagg tgatgtcctt gaacgcctca attccaatct tgctcagatt gcgcacgtag
     5341 cggggaggat ccgagtcggg ttgggtgcca tagctgctgg aaggcttgaa gtaacttgtc
     5401 gatttcttgg ctgcagccgg cttcgcggaa gatgtggaag ctggcttatc cgaagcagcg
     5461 gatttgtcca aggctggaat cgcccatttg taattttcat tcgacggacc gatctcccat
     5521 ttaaagtcct caccggacga tctgcttggt gatttgtccg aatcggacaa tgcagagccg
     5581 gataattcag aaccggatga tgcagcctgg gccatttgcc tcattccctg aagatttgtt
     5641 gaatccacct gcttattgct tttttcatcc gcttgaagcg gttttattgt taaagtgccg
     5701 atcgcgaagg cgaacgcgat tcgccaaaaa aatttcattc ctttctcctg aaccgcccct
     5761 ccgttgtctt ggtcgggatg cggagatctt gctgtatctg cctccagtta tatggtcggc
     5821 tatcttttca aactgctttt ttttaggcac tgaacgtctt gatccaatac cggttcccgt
     5881 cgccgctgcc ttcagtgcac ctcggattgc tacctgccca cacgaagttt ttcgcggcac
     5941 tcgatttgca caacctttcc gtcatgaacc actacctcta ccgagccgta ttcgatattt
     6001 gcaatagtgc tcaggatttg ctccttgata tggggaggaa tccgcttgcc tatgcgtgtg
     6061 cctggatgtg tgctcgaaga gttcctttct tccaggtccg tgattgttgt attccccatc
     6121 attcattcct tgagcggttg aggcacagtc agtacgttat cagtagttca atataattta
     6181 aaataatatc atttcatttg tttatatctt ttttatatat caggataatg caggttttgc
     6241 cccgaacaca cggatttgtt tcaatccaaa aaacgaatga gtatccgcag gcagggcaat
     6301 ccgaggtact gtccaaacct catccgatct gggaaaatga aatctgcttg gtgagaaaag
     6361 aggttttgca atgagcatga tcggtgcgcc ttcgagaaaa gagcagcatg aggttaacaa
     6421 gctgcgcaag cggttgaggc ggctcgtggg tacggctatc gctgatttca acatgatcga
     6481 ggagggcgat cgggtgatgg tgtgcctgtc cggcggcaag gacagttacg ccctgcttga
     6541 tatccttcgt agcttacagg cccacgccca gacacgattc gaactgatcg cggtgaacct
     6601 ggatcagaaa cagcccggct ttcccgaacg cgtgttgccg gagtatctct ccgctatcgg
     6661 catgccgttt cgcattgtcg agcaggatac ctacagtgtg gtcaagcgcc tgattcccga
     6721 ggggaagacc acatgcagct tgtgttcgcg tctgcgccgc ggcgtgctgt accgggtggc
     6781 cggcgaactt ggggcgacaa aaattgcgct gggacaccat cgcgacgata ttctggagac
     6841 gctttttctc aatctctttt atgggggcaa actcaagacc atgccgccca agctggtgag
     6901 cgacgacgga cgcaacatcg tcatacgtcc tcttgcatac tgcaaggaaa aagacttggc
     6961 agcatatgcc gagatggaaa atttccccat cattccctgc aatctgtgcg gttcacagaa
     7021 gaatctccag cgcctggtgg tgggggagat gctggaacat tgggacaaga aataccccgg
     7081 caggttggaa acgatgttca cggccttgca gaatatccag ccctcgcatc tgctcgatcc
     7141 cgcactctat gacttcaagg gactcaagac cggaaacgag ccgttcccgg aaggcgaccg
     7201 cgccttcgac cccgagacat tcgagccggc catggaagaa atcatcagct tgagcaaggg
     7261 cttggaaggc acttgaatca gcacgaccaa ccaactctca aacctgacta catccatgac
     7321 taggtacgga ttcgcttggc tcccggccat attgatcgtc ggtatggtgc tgttgccgtc
     7381 cgctcactcg tcggttcccg tcatggatgg caagaacggc cttcccacac tggcgccgct
     7441 ccttcagaac gtcaccccgg ccgtggtcaa tatttccgta aagacgcgct ccgccctgga
     7501 agagaaccct ttgttccgcg accctttttt ccggcgcttt ttcaacattc cggatcaggc
     7561 agaacgggtc gagcaaagtg cgggctcggg ggtcatcgtg gatgctgcca atggttatgt
     7621 cgtcaccaat ttccacgtca tcaaggatgc tcttcaagtt ctggtgacgc tcaaggaccg
     7681 ccgccagttt caggccaagc tggtcgggac ggacccgggg accgacatcg ctttgctgaa
     7741 aatagacgcc aaaaagttgc aggctttgcg tctgggcaat tcggatctgc tcaatgtcgg
     7801 cgattttgtc gtggccattg gtaacccgtt tggtctggga cagaccgtga cttccggcat
     7861 tgtcagcgca ttgggtagaa gtgggcttga tatcgaaggt tacgaggatt tcatccagac
     7921 cgacgcttcc atcaatcccg gtaattccgg cggcgcactc atcaatctca aaggggaatt
     7981 gatcggtatc aataccgcca ttatcgggcc atccggcgcg aacgtcggca ttggctttgc
     8041 ggtccctagc gtcatggtca aggcggttgt ggatcagatc gcgcgctatg gcgaggttcg
     8101 ccgcggcagg ctcggggcaa gcagtgaaga tatcacgtac gatctggctg gatctctcgg
     8161 actggcttcc accgacggcg ctatcatcag ttcggttgaa cccgattcac ctgctgcaac
     8221 ggccggcttg aaagcgcgtg acgtggtgac gggagtcaac ggaagaagcg ttcggaattc
     8281 catagatttg cgcaacaaga tcgggctgat gccggtcggt gaaatactca acctcgctct
     8341 gatcaggagt ggcaagccct tgactgtgaa ggtcaagatc ggcaaacccc tcgatgtggc
     8401 ctcgaccgag gcggaaaccg ttccccagtt ggcaggtgca accgtagcca atttcaaatc
     8461 cggctcgccg agaggagccg agggggtggt cgtaaccaaa gtcgaaccca acacccctgc
     8521 ctggatgcac gggctgcggc ccggcgacat catcgtgggg gttaaccgca aaaaggttcg
     8581 ctcagtcacg gaatttctca cagccgtaca agcgagccgc gattccgttt atctcaatct
     8641 ggtgcgcggc gatttcaggg ttaccctcat gatccgctag gtactctact tgggggacga
     8701 ttaaattcga caccaaagac gtcatcgcga accgtggaaa cggcctgcgt tggacaagtg
     8761 cgtcttcgcc cactgctgcc cgaggaaact cttctcttta tcgtcatgcg cttgatgtga
     8821 gcgccgttgg agtgatctgt tcactgtata atcccttgtt ttgaccgcag gaaagccggg
     8881 gtttatgcag gaaagatacc gtccagggga agtcgaatca gaagcgcagc ggcattggga
     8941 gcaaagcaag gctttcaagg cagtcgagac tccgggcaag caaaagtact actgcttgtc
     9001 gatgtttccc tatccgtccg gcaagttgca catgggacat gtgcgcaact atacgattgg
     9061 cgatgtgtta tcccgctatt ggcggatgca gggctataac gtgctgcagc ccatggggtg
     9121 ggatgcattt ggtttgcctg ccgagaatgc cgcaatgcaa aaccatgtct cccctgccct
     9181 gtggacctac gacaacattg cctacatgcg caatcaattg cagagcctag gcttggcagt
     9241 tgactggagc cgcgagctgg ctacttgcca gccggattat taccgctgga accaatggtt
     9301 ttttctgag
//
LOCUS       DASSHJ010000129         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_993669, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000129 DASSHJ010000000
VERSION     DASSHJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993669"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4366
                     /locus_tag="VFI43_05140"
     CDS             <1..>4366
                     /locus_tag="VFI43_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET7061544.1"
                     /translation="GELWAIPIMLRLALIENLRRVGAYIAEARNERNLADSWVDKIAG
                     IAESDPKSVVLVIADMARSNPPMTTAFVSELARRLHGQGATLTLPLTWIEQRLAESNQ
                     TVDQMVQLGNQQQAADQVSVSNSINSLRTLGAIDWREFVESVSIVEQTLREDPAGIYG
                     AMEFASRDMYRHVVERVAKVSPMSEGQIARAAVELARDSARDGQNGSNKEGKAIQAHV
                     GFYLIDRGLTELTDAVQVRLSTEEKIQKTGRRHPLLFYLGAIALIVLGLVAGLLARAG
                     GGGVEGWLLVAVGALSIVVMSQLAVALVNWAALFLVKPRALARMDFSKGIPSPFRTLV
                     VIPTLLTSKEEAQSLAEALEVRFLANQDDNLLFGLLTDFADAERETLPEDAPLLDFAG
                     ECIEELNARYAKGGDGLPGEKRSAPACKESPFFLFHRPRRWNPSEGLWMGYERKRGKL
                     ADLNALLRGAGDDAFSAIIGEKSLLAGIKYVITLDTDTQLPRNSAREFVGTMAHPLNQ
                     PRLELIDDECGSIVTGGYGILQPRVGVSLSSTNRSLYAWLFGGEAGIDPYTRVTSDIY
                     QDIFHEGSFVGKGIYDVDAFERALCIRFPENRILSHDLLEGSYARAGLLSDVQLYEEF
                     PSGYLADVKRRHRWIRGDWQLATWLLWRVPAAGHRYQPNPLSSLSRWKLLDNLRRSLV
                     PPASTFLLLLGWIALPDPWFWTLVVVGAMLIPAIIPVLSGLFRKPVEVPWRQQFAMGA
                     QSARRHLGQMLFMLACLPFEAYFSLDAIIRTHVRLLITHRKLLEWSPSREVERLAALP
                     QEETGISRARFFREMWVAPAVAIAGVVGVAVASPWSLIVALPILVLWGGSPALAWWIS
                     RPLPAGGVELSSGQIDFLREVARRTWFFFETFHGPDDNWLPPDNFQEIPSPIIAHRTS
                     PTNMGLALLANAAAYDFGYIGLVELIERINRTMKTMGRMERYEGHFYNWYNTQTLVPL
                     SRYISSVDSGNLAAHLLTLRVALLHAADAPIIGERLFPALHDTSRVLSKAIGGKVTKT
                     LAEFDAALSDASAIRPITLSSIRSHLQALATLSGELVHEMLRERPALPSEAQEWARRL
                     HDQCEKSLRELALLLPQPLPAEAPDESETATGSSAGTGIPSLRELANSGSKEAQEQLE
                     LLEHLAFEAADMAQMEYGFLFNPARRQLAIGYNAGENRLDSSYYDLLASEARLCNFIA
                     IAQEQLPQESWFALGRMLTSQEGEAALLSWSGSMFEYLMPLLVMPTFDNTLLDQTYKA
                     AVDRQISYGKERNVPWGISESGYNAVDIHFNYQYRAFGVPGLGLKRGLGEDLVIAPYA
                     SVLALMVAPEPACRNLQRLTTEGLAGKFGFYEAVDYTPSRLRPGETRAIIRSYMAHHQ
                     GMSLLSLVYALLDRPMQTRFESEPLFQATMLLLQERIPKPAVLRPQVTELPSVRIPKE
                     LPEMPTRILHRPGTPIPEV"
BASE COUNT          898 a         1217 c         1298 g          953 t
ORIGIN      
        1 ggcgagttgt gggcaattcc catcatgctt cgcctggcgc ttatcgaaaa tctgcggcgg
       61 gtcggcgcgt acattgcgga agcaagaaac gaacgcaatc ttgccgattc ctgggtagac
      121 aagattgcgg gcatcgccga aagcgacccc aagagcgttg ttcttgtcat agcagatatg
      181 gcgcgatcga acccgccgat gaccacggct ttcgtctccg aactggccag gcgcctgcat
      241 ggtcaggggg ccactctgac acttccgtta acatggatcg agcaacggct tgcggaatcg
      301 aaccagacag tcgaccagat ggttcagctg ggaaaccagc agcaggccgc cgatcaggtc
      361 tcggtatcca acagcatcaa tagcttacgc accctcgggg ccatcgattg gcgtgagttt
      421 gtggagagtg tgagtattgt ggagcagacg ttgcgcgaag atccagctgg catttatggc
      481 gcaatggagt ttgccagccg cgatatgtat cgccacgtgg tcgagcgcgt cgccaaggta
      541 agccccatgt ccgaagggca gatcgcgcgc gctgcggtgg aactggcccg tgattcagcg
      601 cgcgacggac aaaacggaag taataaggaa ggcaaggcga ttcaggccca cgtcggattt
      661 tatctcattg acagaggact gactgaactg acggatgcag tgcaagtgcg cttgtccacc
      721 gaggagaaaa tccagaaaac cggtcgccgt catccacttc tgttttatct tggggccatt
      781 gcgctgattg tgctgggcct ggtcgcaggc ctgcttgcga gggcaggtgg tggcggcgtg
      841 gagggatggc tgcttgtcgc ggtgggtgcc ctgtcgatcg tggtgatgag ccagttagcc
      901 gttgccctgg tcaattgggc ggcgcttttc cttgtgaaac cgcgtgcgct ggcgcgcatg
      961 gatttctcca agggaatacc ctcgccgttt cgtactctgg ttgtaatccc tacgctgctg
     1021 accagcaagg aggaggcaca gagccttgca gaagcgcttg aggtgcggtt cctcgcgaat
     1081 caggacgaca acctgctctt tgggctgctg acggattttg cggacgctga gcgggaaacg
     1141 ctccccgaag atgcaccgct gctggatttt gcaggcgaat gcatcgaaga gctgaacgcc
     1201 cggtatgcga aggggggtga cggcttgcca ggagaaaaac gttcggcgcc tgcttgtaaa
     1261 gaaagcccat tcttcctttt ccaccgtcca cgccggtgga atccgagcga gggcttgtgg
     1321 atgggctatg agcgcaagcg aggaaaactc gccgacctca atgcactgct gcggggcgcg
     1381 ggggatgatg ctttctcggc gatcattggc gaaaagtcgt tgctggcagg gatcaagtac
     1441 gtgattacgc tggatacgga cactcaactg ccgcgaaatt cggcccggga attcgtgggt
     1501 accatggctc accctctgaa ccagccgcgc ctcgagctca tcgacgacga atgcgggagt
     1561 atcgtcacgg gtggttatgg aattctgcag ccgcgcgtgg gtgtgagcct atcgagcacg
     1621 aacaggtcgc tgtatgcatg gcttttcggc ggagaagccg gtatagatcc gtatacgcgc
     1681 gttacatcgg atatatatca ggacatattc catgagggct cgttcgtagg taagggcatt
     1741 tatgacgttg acgcgttcga gagagcgctt tgcatccgtt ttcccgaaaa ccggattctg
     1801 agccatgatc tgcttgaagg ctcgtatgca cgcgccggtt tgctgagtga tgtgcaatta
     1861 tacgaggagt tcccctcagg atatctcgcc gacgtcaaaa ggcggcaccg gtggattcgc
     1921 ggagattggc agctcgcgac atggctgtta tggcgggtac cggccgccgg acaccgctac
     1981 cagcccaacc cgttgtcatc cctttcccgc tggaagctgc tggacaacct ccggcgcagc
     2041 ctggtcccgc cggcctcaac atttctcctg ctattgggct ggatcgcctt gcctgatcca
     2101 tggttctgga cacttgtcgt ggttggagca atgctcattc cggcgatcat accggtcctc
     2161 tccggtttat tcaggaagcc cgtggaggtg ccctggcggc aacagtttgc gatgggagca
     2221 caatcggctc ggcgtcacct cgggcaaatg ctgttcatgc tggcctgcct tcctttcgaa
     2281 gcttatttta gtctcgatgc cattattcgc acccatgtaa gactcttgat aactcaccga
     2341 aagcttctgg aatggagccc ctcccgggaa gtcgagcgac tcgctgcttt acctcaagaa
     2401 gagacaggaa tcagccgagc acgttttttc agggaaatgt gggttgcccc ggctgttgct
     2461 attgcaggag tcgtcggagt agctgtcgca tctccctggt cattgattgt tgcgctgcca
     2521 attctggtgt tatggggcgg ttctcccgcc ctcgcctggt ggatcagccg gccgcttcca
     2581 gcggggggcg tggagctgtc gagcggccag atagacttcc tgcgggaggt ggcgcgccgc
     2641 acctggtttt ttttcgaaac attccacggc ccggatgata attggcttcc accagacaat
     2701 tttcaggaaa tcccctcgcc catcattgcc caccgcacat ccccaacaaa tatgggtctc
     2761 gcattgcttg ccaatgcggc tgcctatgac tttgggtata taggtctggt cgaacttatc
     2821 gagcggatca accgcacgat gaaaaccatg gggcgaatgg agcgatatga aggtcatttt
     2881 tacaactggt acaacaccca gaccctggta cctctatccc gctacatatc gagcgtcgac
     2941 agcggcaacc tcgcggctca tttattgacg ttgcgcgtgg cgctattgca cgccgcggac
     3001 gcaccaatta tcggcgagcg cctgtttccc gccctccacg atacgtcgag agtcctttca
     3061 aaagcgatcg gggggaaagt aacaaaaact ctcgcggagt ttgacgcggc gctgtcggac
     3121 gcctctgcca ttcgcccgat taccctttcg agcatacgca gtcatctgca ggcattggca
     3181 acattatccg gggagttggt gcacgagatg ttgcgtgaga ggcccgcact tcccagcgaa
     3241 gcacaggagt gggcaagacg cttacatgac cagtgcgaaa aatcactgcg tgaactggcg
     3301 ctcctgctcc cgcaaccact gcccgccgag gcgccggatg agagcgagac ggcgacaggg
     3361 agctctgccg ggacagggat tccttccctg cgggaacttg ccaatagcgg cagcaaggag
     3421 gcccaggaac agttggaact gctcgaacat ctcgcgttcg aggcagccga catggcgcaa
     3481 atggagtacg gcttcctgtt caaccctgcc cgacggcagc ttgcaatagg ctataacgcg
     3541 ggcgagaacc gcctggattc cagttattac gatctgctcg cgtcagaggc aaggctttgc
     3601 aatttcatcg ccattgcaca ggaacaactt ccacaggaaa gttggttcgc tttggggcgg
     3661 atgctgactt cgcaagaggg ggaggccgca ttgctatcgt ggagtggatc gatgttcgag
     3721 tatctgatgc cgctcctggt catgcccacg ttcgataata ccctgctcga ccagacctat
     3781 aaggccgcgg tcgacaggca gatttcgtat ggaaaggagc gcaacgtacc ttggggaatt
     3841 tccgagtcgg gctacaacgc ggtcgacatt cacttcaatt accagtatcg agcgttcggg
     3901 gttcccggcc tggggcttaa gcgcggcctc ggcgaagacc ttgtgatcgc gccatatgct
     3961 tccgtactcg cgttgatggt cgcacccgaa ccggcttgtc gcaatcttca gcgcctcacg
     4021 actgaagggc tggcaggcaa atttggtttt tacgaagcag tcgattatac cccttcacgg
     4081 ctccggcccg gcgaaaccag ggccatcatc aggtcgtaca tggcgcatca ccaaggcatg
     4141 agcctgcttt ccctggttta cgcgctgctc gatcgcccca tgcaaacccg gtttgaatcc
     4201 gaaccgctat ttcaggcgac catgctgctc cttcaggagc gcattccgaa gcctgccgtt
     4261 cttcgccccc aagtgaccga gcttcccagc gtgcggattc caaaggaatt gccggaaatg
     4321 cccacaagaa ttttgcaccg gccggggaca cctataccgg aggtac
//
LOCUS       DASSHJ010000130         9632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_64820, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000130 DASSHJ010000000
VERSION     DASSHJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64820"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..948)
                     /locus_tag="VFI43_05145"
     CDS             complement(163..948)
                     /locus_tag="VFI43_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7061545.1"
                     /translation="MFFLKPVRCRDCKERFWVKNPSAYFSVAALLVFSALVVTTIWLI
                     IDPNMDSAFTVEASSNPAEPPSIPGAPSPGKLAKNAEEKRANVGSVPPPLNLSQPPGQ
                     ASQPPKKTRDDHQFTVQLYQDSAENGEPDAQYKLGLLYLTGNGALQDFAEAAKWMKRA
                     AEQGYALAQYELGMIYRTGYGFSVDPVKSYMWLNLAAAAGIQQAVGARDEVMRSLNSK
                     QLAQAQKISREWLTSRSRRGVAGKSAGRSDPDPDAETMGDGEP"
     gene            complement(1222..2157)
                     /gene="epsC"
                     /locus_tag="VFI43_05150"
     CDS             complement(1222..2157)
                     /gene="epsC"
                     /locus_tag="VFI43_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET7061546.1"
                     /translation="MPEDLSSTRTNLEIGQVVEELRTLRLRALENRQRRHKPPKLPSR
                     KVLVGIADGLRAVLFPNRLGMPDLTDESVDYYVGHMLDVTLRDLLAQVRRELRFVSGL
                     DGASNADREQAAEIIRAFAKRLPHIRSLLESDIQAAYEGDPAARSIDEVLVCYPGVTA
                     ITHYRLAHELYCLGAPLIARMISEIAHSATGIEIHPGAHIGGSFFIDHGTGVVIGETA
                     VIGEHVRLYQAVTLGAKRFPVDEHGHLVKGNLRHPIVEDDVVIYAGATILGRITIGRG
                     SVIGGNVWLTRSVPPGSNISQAQTRNEVFGDGSGI"
     gene            complement(2172..3149)
                     /gene="cysK"
                     /locus_tag="VFI43_05155"
     CDS             complement(2172..3149)
                     /gene="cysK"
                     /locus_tag="VFI43_05155"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7061547.1"
                     /translation="MSYWFNDNSQSVGNTPLVRLNRIAGDAPATVLAKIEGRNPAYSV
                     KCRIGAAMIEDAESRGLLRPGKELVEPTSGNTGIALASVAAARGIPLTLTMPETMSVE
                     RRKLLSAYGAKLVLTSGSLGMKGAVAKAEEIVASDPERYLLLQQFTNPANPAIHERTT
                     GPEIWNDTRGLIDIFISGVGTGGTITGVSRHIKKTKEKAIISVAVEPAASPVLSQERA
                     GLPLTPGPHKIPGIGAGFVPKNLDLSLVDEIEQVSNEEAILYARRLAREEGIISGISC
                     GAAAAVAVRYAIRPENQGKLIVVILPDSGERYLSSILFEDVYDTGEFLQ"
     gene            3534..4892
                     /gene="aroA"
                     /locus_tag="VFI43_05160"
     CDS             3534..4892
                     /gene="aroA"
                     /locus_tag="VFI43_05160"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7061548.1"
                     /translation="MNFIDLPVARTAQGVIRLPGSKSISNRVLLLAALAAGTTDVRDL
                     LISDDTTRMLDALRTLGVAVAEIDANHYRLQGVGGQFPFRFPVAEADLFLGNAGTAFR
                     PLTAVLALAGGKYRLSGVPRMHERPIGDLVDALRQLGANISYLGKTGFPPLQIKSSTI
                     HPATITVKGDVSSQFLTGLLMALPFSAPATGEKPTINVLGELISKPYVELTMALMARF
                     GVEVERDGWKSFSLHAGQSYRSPGEIFVEGDASSASYFLAAGAIGKGPVRVEGVGKGS
                     VQGDLRFIDVLDKMGAEITLGDNWIEAAAPPAGYLQGVELDCNHIPDAAMTVAVTALF
                     ARGTTTLRNIASWRVKETDRLSAMACELRKLGATVEEGIDFLRITPPVHGLASGAEID
                     TYEDHRMAMCFSLASLGTAVRINDPGCVAKTFPDYFDKFASIVHSSKGPAWEVARVCE
                     ER"
     gene            4892..5572
                     /gene="cmk"
                     /locus_tag="VFI43_05165"
     CDS             4892..5572
                     /gene="cmk"
                     /locus_tag="VFI43_05165"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7061549.1"
                     /translation="MTTCSAPVIAIDGPSASGKGTVAQRVAAKLGFHYLDSGALYRLV
                     ALAAMQSGVDLANECALGEVAAKLDVRFDGETVWLSNEDVTYAIRAEACSNAASKIAP
                     YPFVRAALLARQREFRNAPGLVADGRDMGSVVFPDAVLKIFLTASPETRAERRYKQLI
                     EKGIDANMATLLQDIRERDRRDSNRTLAPLQMGEDSNLLDTTSLNITEAVDCVLKQYA
                     DIRTKKHL"
     gene            5855..7579
                     /gene="rpsA"
                     /locus_tag="VFI43_05170"
     CDS             5855..7579
                     /gene="rpsA"
                     /locus_tag="VFI43_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7061550.1"
                     /translation="MTTASTALSTIDSPESFATLFEESLSRQEMRVGEVITAQVVRVD
                     YNVVVVNAGLKSESFIPVEEFKNDRGEIEVKPGDFVSVAIEALEDGYGETRLSRDKAK
                     RLTAWHDLEAAMESGAVVQGLVTGKVKGGLTAMINGIRAFLPGSLVDIRPVKDTTPYE
                     NKEMEFKVIKLDRKRNNVVVSRRAVLEETQGADRQTLLETLTEGAVVKGIVKNITDYG
                     AFVDLGGIDGLLHITDLAWRRVKHPSEVISVGDEVTAKVLKFDQEKNRVSLGMKQLTE
                     DPWVGLSRRYPPHTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVYPSKVV
                     QLGDEVEVMILEIDEERRRISLGMKQCKVNPWEDFAMTHQKGDKVRGQIKSITDFGVF
                     IGLPGGIDGLVHLSDLSWNQPGEEAVRNYKKGDEVEAVVLSIDVERERISLGIKQLEG
                     DPFNSFVSVNDKNSIVKGTVKSIDAKGAVIALENDVEGYLRASEVSRDRVEDIRTRLK
                     EGDAVQAMIINVDRKNRTINLSIKAKDLAEESDAIHKVAADSSATAGTTSLGALLKAK
                     MDVKNTEQ"
     gene            7589..7873
                     /locus_tag="VFI43_05175"
     CDS             7589..7873
                     /locus_tag="VFI43_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET7061551.1"
                     /translation="MTKSELIARLAARYPQLVAKDAELAVKVILDAMAKSLSEGQRIE
                     IRGFGSFDLNYRPPRVGRNPKSGEKVRVPEKYVPHFKAGKEMRERVDFKS"
     gene            7974..8228
                     /locus_tag="VFI43_05180"
     CDS             7974..8228
                     /locus_tag="VFI43_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET7061552.1"
                     /translation="MRYPILFFRILLFLLLLGFAIRNAEPVTLRYYFGYEWQAPLVLI
                     MFLFFALGIVVGVASCFAKLFRQRREILDYRKKYDIKDEH"
     gene            8256..8978
                     /gene="pyrF"
                     /locus_tag="VFI43_05185"
     CDS             8256..8978
                     /gene="pyrF"
                     /locus_tag="VFI43_05185"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7061553.1"
                     /translation="MSDPRIIVALDFPAAELATSFAARLNPGMCKVKVGKELFTASGP
                     RAVESLIDKGFDVFLDLKFHDIPNTVATACKAAARLGVWMMNVHALGGRKMLAAAREA
                     VPAGSTRLIAVTLLTSMGSSDLRDIGISGEPQDVVQRLALLARDCGLDGVVSSALEAT
                     ELRKVIGNDFCLVTPGIRPAGTSPDEQKRITTPGQAIKNGANYLVIGRPITQASDPVL
                     VLQQLNDEIASLLRSNTPKIDP"
     gene            9152..>9632
                     /locus_tag="VFI43_05190"
     CDS             9152..>9632
                     /locus_tag="VFI43_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7061554.1"
                     /translation="MFDYHVLPSQRLAGGVRLVSPLLPKPVTLSSPALEVMTDLRLVP
                     AGVTEPDSTMESANVFMMQRGIRSLFVMNRDNVLCGVITASDILGEKPLRFIQERQLK
                     HSEILVSDIMTPVDRLEAIPMKIVESSRVGNVIATLRESGRQHAFVMESSTDGTPVVR
                     "
BASE COUNT         2176 a         2514 c         2701 g         2241 t
ORIGIN      
        1 tttcatgaac gattcggtaa atcaaggtca ggtttcatca taaagattgg gtcagttccg
       61 ccccggatag aatttaaaga aactgatgta accaatatac gccatcagtc tgccggacgg
      121 ttcgaaacaa tgactggaat gggtcgccag aataatatcg aactatggct cgccgtctcc
      181 catcgtttca gcatctgggt cggggtccga tctgcctgcg cttttgccgg ccacgccccg
      241 ccgcgagcgg gacgtgagcc attcgcggga aattttttga gcctgtgcaa gctgcttgct
      301 attcagcgat cgcatgacct catcgcgcgc accaaccgcc tgctgaattc cagccgctgc
      361 cgcgagattg agccacatgt agctctttac aggatcgacg gaaaaaccat accctgtgcg
      421 ataaatcatt ccaagctcat actgagccaa ggcgtacccc tgttccgccg cgcgtttcat
      481 ccactttgca gcttcagcaa aatcctgaag ggcgccattg ccggtcagat agagcagccc
      541 gagtttatac tgggcatcag gctcgccatt ttccgcactg tcctggtata actgaacagt
      601 aaactggtgg tcgtccctgg ttttttttgg aggttgggaa gcctgacccg gcggttgcga
      661 gaggttcaaa ggcgggggga cggagccgac gttggcgcgc ttttcctcag catttttagc
      721 cagtttgcca gggctcgggg cgccaggaat ggacggtggc tctgccgggt tgctggatgc
      781 ttcaaccgta aaagccgaat ccatgttcgg gtcgatgatg agccagatcg tggtgacgac
      841 cagggcggaa aagaccagca atgctgcaac gctgaaatac gcgctgggat tttttaccca
      901 gaaacgttcc ttgcagtccc tgcatcttac cggtttgagg aaaaacattc ctcctgtacg
      961 ttcgcccgct cttacacggg atctgtgcgt attttcgctt ccgcacttaa tgcattttgc
     1021 gtacatgagg ctccaacccg ttcccttttc tgatgttttt gcacagtcta agttttggcc
     1081 gcataaccgt caactggact ttagcgcact gtctttatga aaatggtgag cgggaagccg
     1141 gtgattaact ggtctcgcca atcttcattt tggcacgttc ggagtggttg agttcccgtc
     1201 cgaactagct gctcacatcc cctagattcc gctgccatcg ccaaatacct catttcgagt
     1261 ctgtgcctga gatatgttgc tgccgggtgg cacactgcgt gtgagccaca cgttgcctcc
     1321 gattaccgaa ccgcgcccga ttgtaatccg gccaagaata gtggcgccgg catagatgac
     1381 cacgtcgtcc tctactatgg gatggcgaag atttcccttg acaaggtgac cgtgctcatc
     1441 gacggggaag cgctttgccc cgagcgtcac cgcctggtaa agacgcacgt gttccccgat
     1501 cacagcggtt tcgccgatga caacgcccgt accgtgatca atgaaaaagc ttccgccaat
     1561 gtgcgcgccc ggatggattt caatgcccgt cgccgaatga gcaatttccg aaatcatgcg
     1621 cgcgatcaag ggagccccca gacagtacag ttcgtgggcg agccggtagt gggtgatggc
     1681 agtgacaccg ggatagcaga ccaggacttc gtctatgctc cgggcagcgg gatcgccttc
     1741 ataggccgcc tggatatcac tctcgagaag actgcgtatg tgtggaagac gttttgcaaa
     1801 agcacgaatg atctccgcgg cctgctcgcg gtccgcgtta ctcgccccgt cgagtcccga
     1861 tacaaaccgc agttcgcggc gaacttgcgc caggagatcg cggagtgtca catccagcat
     1921 gtgcccgaca taatagtcca cgctttcatc cgtcaggtca ggcatgccca gccgattagg
     1981 gaaaagcaca gcccgcaatc catcggcaat gccaaccagg accttacggg aagggagttt
     2041 cggcggtttg tggcgccgtt gcctgttttc cagcgctctc aggcgcaagg tgcgcagttc
     2101 ttctacaact tggccaattt ccagatttgt cctagttgat gataagtcct cgggcattcc
     2161 agtctccacc gttactgcaa aaattcacct gtgtcgtaca cgtcttcgaa taggatggag
     2221 ctcagatagc gctcccccga atcaggcagt atgacgacaa tcagctttcc ctggttttcg
     2281 ggccgaatag catagcggac agccacggca gcggcggcac cgcaagagat tcctgaaata
     2341 atcccttctt cccgggcaag acggcgcgca taaagaatcg cttcctcatt gctgacttgc
     2401 tcgatttcgt cgacgagtga cagatcgagg tttttgggca cgaagcccgc tccgattccg
     2461 ggaatcttgt gggggcccgg tgtcaacggc aagccagctc tttcctgact caagaccggg
     2521 ctcgcggcgg gctcgaccgc aaccgagata atcgctttct ctttcgtttt tttgatgtga
     2581 cgcgagacgc cggttatggt gccaccggta cctacacctg aaatgaaaat atcgatgagg
     2641 cctcgcgtgt cattccagat ctcgggtccg gtcgtgcgct cgtgaatagc ggggttcgcg
     2701 ggattggtga attgctgaag caacaggtac cgttctggat cagacgcgac aatttcctca
     2761 gccttcgcta ctgccccctt catccccagc gagcccgatg taagaaccag tttcgcccca
     2821 taggcggaaa gaagcttgcg ccgctcgacg ctcatggttt ccggcatggt cagggtcaac
     2881 ggtattccac gtgccgcggc gacggaggca agtgcgattc ccgtgttgcc gcttgtcggt
     2941 tcgaccagtt cttttcctgg acgaagcaaa cctcgcgact ctgcatcctc gatcatagcc
     3001 gcgccgatgc ggcatttaac agagtaagcg gggttgcgcc cctcgatttt tgccaatacc
     3061 gtagccggcg cgtcccccgc aatacgattg agccttacca aaggcgtatt gccaacagat
     3121 tgcgagttat cattaaacca gtacgacatc ttgtaggcct ttcgttggaa tttgtattct
     3181 tcaggccatc gcaaagatca ggccagccgg catatggcat ttggcatgaa tataaacgcg
     3241 atatatcctc cgaatgaatg attttaacgc ctgcgtccca gagatcgcga aaaggaaccg
     3301 gatatttttg cgatcactgc tgacgcggtc aacagtgaca ttgttccgag gagggtggta
     3361 aagggaagat cagatgcaaa tcgggaaagc aggcggcctc gtaatgttaa aatgctccgt
     3421 ttaattaagc cgctgccggg tacccaccaa ggcagtttcc cgccaatccg ttcccaataa
     3481 atcaaaccgt catacggttt cgatccgccc tgccaacgct gccgacaata taaatgaact
     3541 tcatcgatct tccggttgct cggaccgcgc agggcgttat acgcctcccg ggctcgaaga
     3601 gcatttccaa ccgcgtcctt ttgcttgctg cgcttgccgc aggcacgact gacgttcgtg
     3661 atctgctgat ttccgacgat acgacaagaa tgctcgatgc ccttcgtacg ctcggagtcg
     3721 ccgttgcaga gatcgacgcg aatcattacc gcttgcaggg tgtaggaggt cagtttccat
     3781 ttcgcttccc ggttgcagag gccgatttgt ttctcgggaa tgctggaact gcttttcgtc
     3841 cactgacagc cgtgctggct ctggcggggg gcaaataccg gttatcgggt gtgccgcgga
     3901 tgcacgaacg cccgatcggg gacctggtcg atgcactccg gcaattgggc gcaaacatca
     3961 gctatctcgg taaaacaggt tttcctccac tgcaaatcaa atccagcact atccatcctg
     4021 ccacaataac ggtaaaaggc gacgtgtcca gccaattcct gaccggactg ctcatggcgt
     4081 tgccgttttc tgcacccgct accggtgaaa aaccaaccat aaatgtcctt ggagagctca
     4141 tctcaaagcc gtatgtcgaa ctcaccatgg ccctgatggc ccgctttggc gtggaggtcg
     4201 agcgcgatgg atggaagtct ttttctttgc atgcaggcca aagctatcgc agtcccggcg
     4261 agatttttgt cgaaggggac gcatcttccg cttcatattt tctggccgct ggcgcgatcg
     4321 gcaaggggcc cgtgcgagta gagggcgttg ggaagggaag cgtccaggga gatttgcggt
     4381 ttatcgatgt cctggacaag atgggtgcgg aaatcacgtt aggcgacaac tggatagagg
     4441 cggcagcgcc cccggccggt tacctgcagg gggttgaact cgattgcaat catatcccgg
     4501 atgccgccat gacggtggcc gttacagccc tcttcgcacg cggaacgact acgctcagga
     4561 acattgcaag ttggcgcgta aaggaaacgg atcgtctctc tgccatggcc tgcgaattgc
     4621 gaaagctggg cgcgaccgtg gaggaaggca ttgattttct tcgcatcacg ccgccagtcc
     4681 atggattggc ttccggcgct gagatcgata cctatgagga tcaccgcatg gctatgtgtt
     4741 tctcgctggc aagtttaggt actgcggtgc ggatcaacga tcccgggtgc gtggcaaaaa
     4801 cttttccgga ttatttcgac aagtttgcat cgatagttca ttcctcgaag ggcccggcat
     4861 gggaagttgc gcgagtttgt gaggaaagat gatgaccact tgcagtgcac ccgttattgc
     4921 gatcgatgga ccttcagcat ccggaaaggg taccgtagct cagcgagtgg cggccaaact
     4981 ggggtttcac taccttgaca gcggcgcact ttaccggttg gtggcattgg cagcgatgca
     5041 atcgggagtg gacctagcca atgaatgtgc gctgggcgaa gttgctgcga agcttgatgt
     5101 gcggtttgac ggtgagacag tttggctgtc gaatgaggat gttacctatg ctatacgcgc
     5161 tgaggcgtgc agtaacgctg cttcgaaaat cgctccttac ccattcgtga gggcagcgct
     5221 gttggcgcgg caacgcgagt ttcgcaatgc ccctggcttg gttgccgatg gacgggatat
     5281 gggatcagtt gtttttcccg acgccgtcct caagattttt cttaccgcaa gccccgaaac
     5341 acgggccgag cgacgatata agcagttgat cgaaaaaggt atagatgcta atatggcgac
     5401 ccttttgcag gatatacggg aacgcgacag gcgtgacagt aatcgaaccc tagcgccctt
     5461 gcaaatgggc gaggattcaa atctactgga cactacttcg cttaatatca ccgaagcagt
     5521 agattgcgta ctaaagcagt acgctgatat tcgcacaaaa aagcatctct agaacttgag
     5581 ttccaggcca tgctctccac ttgccggtca tctttagccg gcctcgattg gcggagcgat
     5641 agctccttgc atggcaaaaa tttcccggac acctagcggt tacagcgttg tacgggattg
     5701 gacacttatt ttttcgtgtc aaaaagctga ttgctgcgcc gggctgtctc gatgcatgct
     5761 cgcaacagga gaatgcatcg ggaccgtttt ttattaatct ttaaccccgt catttacctt
     5821 atggacggga tttacaaacc aggtattttt aacgatgacc actgcttcca cagccctttc
     5881 aaccatagat tcccccgaaa gtttcgcgac cctctttgaa gaaagtcttt cccgccagga
     5941 aatgcgggtg ggcgaagtta tcaccgctca agttgtccgc gtcgactata acgtcgtcgt
     6001 cgtaaacgcc ggccttaaat ccgaaagctt cataccggtc gaagaattca agaatgacag
     6061 gggagaaatc gaagtcaagc cgggcgattt tgtaagcgtt gctattgaag cccttgaaga
     6121 cggttatggc gagacccgcc tgtcgcggga caaggcgaag cgtctgactg cctggcacga
     6181 ccttgaagct gcgatggaga gcggcgcggt tgtgcagggt ctggtgaccg gcaaggtcaa
     6241 gggcggcctc accgccatga ttaacggcat tcgcgctttt ctccctggct cgttggtgga
     6301 tatccgtcct gtgaaggata cgacgcccta tgaaaacaag gagatggagt tcaaggtgat
     6361 caaactcgat cgcaagcgca acaatgtcgt ggtgtcgcgt cgtgcagtcc tggaggagac
     6421 ccagggtgcg gatcgtcaga ctttgctgga aactctgacc gaaggcgctg tggtcaaggg
     6481 tatcgtcaag aacatcaccg attacggtgc ctttgtggac ctcggcggta tcgatggctt
     6541 gctgcatatc accgatctcg catggaggcg tgtcaaacat ccttctgaag taatcagcgt
     6601 aggcgatgaa gtcacggcca aggtattgaa gttcgaccag gaaaaaaacc gcgtatcgct
     6661 gggaatgaag caattgacgg aagacccgtg ggtggggttg tcgcgccgct accccccgca
     6721 cacccgtttg ttcgggaagg tcagcaattt gacggattac ggcgctttcg tggagatcga
     6781 gcagggcatc gaagggcttg ttcacgtatc cgaaatggat tggaccaaca agaatgttta
     6841 tccgtccaaa gtggtgcagt tgggcgatga agtcgaagtg atgatcctgg aaatcgatga
     6901 ggagcgccgg cggatttctc tgggcatgaa gcaatgcaag gtcaacccgt gggaggactt
     6961 tgcaatgact caccagaaag gcgacaaggt gcgcggccag atcaagtcca ttacggactt
     7021 cggggttttt atcggtttgc ccggcggcat agacggcctg gtgcatctgt cggacctttc
     7081 gtggaaccag cccggcgaag aagcggtgcg caactacaag aagggcgacg aggtggaggc
     7141 tgtcgtattg tccatcgacg tggagcgcga acgcatctcc ctgggtatca agcagcttga
     7201 aggcgatccg ttcaacagct ttgtttctgt caacgataaa aacagtatcg tcaaaggcac
     7261 ggtcaagtcc attgacgcga aaggagctgt cattgcgctg gagaatgacg tggaaggata
     7321 cctgcgcgcc tccgaagtct cacgcgaccg ggttgaagac attcgtacgc ggttgaaaga
     7381 gggcgatgcg gttcaggcga tgataatcaa tgttgatcgc aagaaccgga ctataaacct
     7441 ttcgataaag gcgaaggatc tggcggaaga atcggacgcc atacataagg ttgcggctga
     7501 tagttcggca accgccggga ctacgagcct gggggccctt cttaaagcca agatggatgt
     7561 aaaaaacacc gagcaataag ggaatttcat gacaaagtct gaattgatcg cgagacttgc
     7621 tgcgcgctat ccccaactgg tggcaaagga cgccgagctc gcggtcaagg taatacttga
     7681 tgcgatggcc aagagtttgt cagaaggtca gcgtatcgaa attcgcgggt ttggcagctt
     7741 tgatctgaac tatcgtccgc cgcgggtggg ccgaaacccg aaatcagggg aaaaggtacg
     7801 ggtgcccgag aaatatgtac cacatttcaa ggcgggaaaa gaaatgcgcg agcgggtgga
     7861 tttcaaaagc tgaagagttg agaattggtg gataataagg cggcggatca aaagatcacg
     7921 ccgccttttt aattgagcga atataaccag tctggtgctt agcggagctt tgcgtgcgct
     7981 atccgattct cttctttcgc atcctgttat ttctgttgct gctgggtttc gccatcagga
     8041 atgcggaacc cgtaaccctg cgttattact ttggctatga atggcaagcg ccccttgttc
     8101 ttatcatgtt cttattcttt gcgctcggca tcgtggttgg cgttgcgtca tgcttcgcaa
     8161 aattgttcag gcagaggcgg gaaatcctgg attaccggaa gaaatatgac atcaaggatg
     8221 agcattaacg gattggcgag gtcgatgcct gacctatgag tgatccccgc attattgtag
     8281 ccctggattt tcccgccgcc gaattggcga cttccttcgc agcgcggttg aacccgggca
     8341 tgtgcaaagt aaaagttgga aaagagcttt ttacggcttc cggcccccgg gctgtggaat
     8401 cgttaattga caaagggttc gacgtttttc tcgatctaaa atttcacgat attcccaaca
     8461 cggtcgcaac cgcctgcaag gccgctgcaa ggctgggtgt atggatgatg aatgtccacg
     8521 ccttgggggg aagaaaaatg cttgctgccg cacgggaagc agtgccggca ggctccacca
     8581 ggttaatagc tgtgaccttg ctgaccagca tgggctcttc cgacctcagg gatatcggga
     8641 taagcggcga gccgcaggat gtcgtgcaac gcctggcgct gttagcccgg gattgcggct
     8701 tggatggcgt agtcagttcg gcattggaag cgacggaact caggaaggtg attggtaacg
     8761 atttttgtct cgttactcct ggaatacgcc cggcaggcac ttcccctgat gagcagaaac
     8821 ggatcaccac tcccggtcag gcgataaaga acggtgccaa ttatctggtc atcgggaggc
     8881 cgataacgca ggcgagcgat ccggttctgg ttttgcagca actgaatgat gagattgcct
     8941 ctctcctacg atccaacaca ccaaagatcg atccatgaag accgcatgct cctgctgcac
     9001 gcgccccttc tacaggcagc agctactcga caaagccatg gcaagccagt aaggatggct
     9061 tgtttttcgg ataaagaccc tactacaagc tatacttatg taagattcct ttattccgat
     9121 atcaattcca tatacgcgag aggatagcgc catgttcgac tatcacgttc ttccctcaca
     9181 acgcctggct ggcggtgtgc gtctggtttc tcctctattg cccaaacccg ttaccttgtc
     9241 ttcgccagcg cttgaggtca tgacggattt gcgtcttgtc cccgcggggg tcacggaacc
     9301 cgattccacg atggagtccg ccaatgtctt catgatgcag cggggaatac gttcgctgtt
     9361 tgtaatgaac agggacaatg tcctttgcgg tgtcatcacc gcaagcgaca ttcttggcga
     9421 gaaaccgctt cgcttcatcc aggaacggca actaaaacac agtgagattc tggtgtcgga
     9481 catcatgacg ccagtggatc ggctcgaagc cattccaatg aaaatcgtcg agagctcaag
     9541 ggtcggtaat gttatcgcaa ccttgcgcga aagcgggcgt caacacgctt ttgtcatgga
     9601 gagcagcacc gatggaacgc cggtggtgcg gg
//
LOCUS       DASSHJ010000131         6553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_816142, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000131 DASSHJ010000000
VERSION     DASSHJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816142"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..504)
                     /locus_tag="VFI43_05195"
     CDS             complement(223..504)
                     /locus_tag="VFI43_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HET7061555.1"
                     /translation="MASGKSGGDTSKRGFASMDEQKQREIASKGGKAAHAKGTAHEFD
                     SEEAREAGRKGGEASHGGHRGRSSGSNSSSRSNSSPQHAKSGSHSHKQG"
     gene            complement(732..1622)
                     /locus_tag="VFI43_05200"
     CDS             complement(732..1622)
                     /locus_tag="VFI43_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7061556.1"
                     /translation="MSPSLKPLNQQVIVVTGASSGIGLCTAKLAAARGAKVVLIARSE
                     ETLEKVAGEIQAVGHEAMYFATDVADRKKMEAAARKAVECFGRIDTWVNDAGVSIYGR
                     LDEVSDADSRRLFETNFWGVVNGSLVALPYLKAHGGALINLGSEVSEAVVPLQGMYSA
                     SKHAVKGFTDVLRVEVEELEKAHISITLIQPTAVDTPFPHHARNYMEREPKLPPPLID
                     PEDVAEAILKAATEGGRDVKVGAMAVVNTAVAKLIPSLGDKMSAKRGSDQQEKIPPVH
                     PEGILYHPGESGSEHGGAPI"
     gene            2331..2606
                     /locus_tag="VFI43_05205"
     CDS             2331..2606
                     /locus_tag="VFI43_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061557.1"
                     /translation="MNKFISTVILTAFTAVFSFGATAADSSNDNTTGNSSDKKEKWEK
                     KYGKGGKGDDSTSEIMKNKEPKDLSPDEKYEYRDQETMNKKPYKQTK"
     gene            complement(2754..3119)
                     /locus_tag="VFI43_05210"
     CDS             complement(2754..3119)
                     /locus_tag="VFI43_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061558.1"
                     /translation="MSYMCSVSVLRPVLLAGLLTCVSFVHAQATGPAGGVQSGMSGAQ
                     SAQGPALAGQGSSAGGSRATTGAMPEGAGASTEKGTSRNIGLSGDYSKPRVSGSAGMG
                     SDESRGASTPGTGVGSVGF"
     gene            complement(3229..6087)
                     /locus_tag="VFI43_05215"
     CDS             complement(3229..6087)
                     /locus_tag="VFI43_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7061559.1"
                     /translation="MIKREVIRGMDQAATERSSVPGWLGGTEMGELIFTHDWTDSGLG
                     PAQDWPPQLQLAVNIMLRLPFAALLLWGENLVQIYNDGFRNLVGSKHPRGLGQKVSEC
                     WPEVWDFVEPACAAVMQRAESLTFDEQRFVLNRNGVPSESFFKLTYSPVPGTMPEEIG
                     AKGGSPGGVLVTVIETTEVIQAREREAERVRLREVAQANRIQLLEEVFSVAPSFIFVL
                     RGPDLTFEFANNACYLLIGQDDLIGRSYSAVIPESAPDGCHEHLAHVMATGKPFIGSE
                     MRMSLARAPGIAPEERFIDLVYLPLLDEDGVCERVLAHGIDVTAHVQKRKKAEEALCK
                     REEHFRRALEVETVGVIFFDTKGEITEANEAFLTMSGVSGTERAEGLLRLDVHTPPEW
                     AELLLRATGGCETGGHTGYYEQEFVRENGSTWWAAFTAKQLNALESVGYVTEITRRKR
                     AEQRLRESEARFRTMAEASPALTWQTDERGGLVYLNQRCNELMGRSVEELIPWGWRSI
                     LHPEDGTDYIAAFEQAVCDRSRFQKRVRVRKPDGTLRWLKSYALPWFGVEGDYAGHVG
                     VSIDITDTVNAENALREAGRLKDEFLATLAHELRNPLAPVASALTLLEHSDNSAAVPH
                     LLSIISRQVNYMVRLVDDLLEVSRITSGKVELRCAPTDLAAVLGDVTEACQPVIAEKK
                     HQLSISILKVPLMVYADSVRLGQVFTNLLNNAARYTPHDGHIWLSAREEGSYAIVSVR
                     DDGIGILPGMLPRLFDMFSQERRRGIGTQEGLGIGLNLVHRLVSMHGGTVEAMSNGKD
                     QGSEFVVRLPLAQAPNTDTSPHPDTVSHEPEGLRLLVVDDNQDAAEVLQMLLASMGMN
                     AWAVNSGRDALSAIPDFKPNVILMDIGMPGMSGHEVARHIRSRPEFNDITLVALTGWG
                     QEEDRRQSKASGFNHHLTKPVDLKTLKELLASIQGTSE"
BASE COUNT         1497 a         1894 c         1666 g         1496 t
ORIGIN      
        1 gccttgaatg tcgcctgcag ggctgccagc gaaataatca gtgccgcgca ggcttcgacc
       61 ccggttgcga gatgcaccgt gtaggatttg atcaattcgt tcatggcgga agcattggag
      121 cagcgagtgg ccctcaagaa tgcaatgcca ggtgaggagc atgtctcaat gaaaaaaggt
      181 gagataaact cacctttttc cgggattcag cgcgctgcca atctagcctt gcttgtggct
      241 atggctgcca gattttgcat gctgagggga tgaattgctg cgagatgaag aattactgcc
      301 ggagcttcta ccgcgatggc cgccatgaga tgcttcgcca cctttccgcc cggcttcacg
      361 agcctcttcc gaatcgaact catgcgcggt ccccttcgcg tgcgctgcct ttccgccctt
      421 gctcgcaatc tcccgctgtt tctgctcgtc catggacgca aatccgcgct tgctggtatc
      481 gccgccactt tttcctgagg ccatatgagt ctcctgtatg caagaatacg aaaaaattcc
      541 gttgacatta cgttcgccat gaagtcaatg ccttcggaaa cgttatcttc gacatcagcc
      601 gagatgtaat ttcagcttaa tgaacggccg tgctttttta tgttcggaga cgcacacaaa
      661 cttaaaaaaa tcaagccttt ccccgaatgc gaaggccatg atgaatgcat tcgcccgaat
      721 ccaaccggat gtcatatagg cgcgcccccg tgctccgatc ccgattctcc ggggtgatac
      781 aggatacctt cgggatgaac gggaggaatt ttttcctgct gatcgcttcc ccgctttgca
      841 gacatcttgt cgccaagact agggatcagc ttggctaccg cagtgttgac aactgccatt
      901 gcaccgactt tgacgtcacg ccctccctct gtggcggctt tcaggattgc ttcggctaca
      961 tcctcgggat cgatcaacgg aggcggcagt ttaggctccc tttccatgta gtttctcgcg
     1021 tgatggggaa agggagtatc gacagcggtg ggctggatga gtgttatcga tatatgcgct
     1081 ttctcgagtt cttcaacttc aacccgaagg acatcagtga accctttgac tgcgtgcttg
     1141 gaggctgaat acatgccttg caaaggcaca actgcttcgg atacctcact gcctaaattg
     1201 atgagtgctc caccatgcgc tttcagataa ggcaacgcga ccagtgaacc attcacgact
     1261 ccccagaaat tagtctcgaa caacctccgg ctatcggcgt cactcacttc gtccaagcgg
     1321 ccatatatcg aaacgcccgc atcattgacc caggtatcga tccggccaaa gcattccacc
     1381 gcttttcgcg ccgcggcctc catttttttt ctgtccgcga catctgtggc aaagtacatc
     1441 gcctcgtggc caaccgcctg gatttctccc gccactttct ccagtgtttc ctcgctgcgg
     1501 gcgataagta ccactttggc tcctcgcgca gctgccagct tcgcggtgca aagaccaatg
     1561 ccgcttgaag cgccagtaac tacgatgact tgttgattta aaggtttaag agaaggactc
     1621 atacgtttac tcctcgaatt tatatttctc tcagtcaagt tttctatagc aagagcaaaa
     1681 aacattcaaa tgctactgct gcgatatttt tgagttgtcc cgtggctcaa tcgtggtttt
     1741 tcaggctaca aatttattgt gtcccggact gtgcacaaac gaacaaagtt tttcgattcg
     1801 atccggttag ggatgccggg aaactattgg aggaagcatt cgaaaccagg gttgagtgag
     1861 caatctgttg aggaaagcgt ccggtcatgg agagtaccgc tccgaatgga ggccgggaga
     1921 ggcatggcaa tcacgccggg acaggagaga aaagactgga cgcaggtaag agaggaaaga
     1981 ccggaggagg cggtaactcc ttttgagcta tagccggggc ggtcgccggc agcgcccgcc
     2041 gttcccgctt ttccttgtgc tgaacatctt cattcggctt atccctctaa gggtcggaat
     2101 agcattaagg cttgatggcc aggtccggcc attccggcag caattcgacg ctatcgtgcg
     2161 ttagcgcact gagtatatcg aagagtttac gtatcgttcc cgcatgtaat gaaggggaaa
     2221 cgagcgggag gcctggctgc gctttggccg ttaaaactcg cgtaaagcaa cactgcgcac
     2281 aacgatgttt ttatctacca ctgcaactca ctcataggaa ggagattaaa atgaataaat
     2341 tcatatctac tgtgatcctg accgctttca cggctgtttt cagcttcggc gcgaccgctg
     2401 ctgatagttc gaacgacaac acaacgggta actccagcga caagaaagaa aaatgggaaa
     2461 agaagtatgg aaagggtgga aaaggggatg actcgacctc agagatcatg aaaaacaagg
     2521 agcctaagga tttatcgccg gatgagaaat acgaatacag agatcaggaa acgatgaaca
     2581 agaaaccgta taagcagacc aagtagtgat tttttcgatc gatatggaaa aacagaggaa
     2641 ccatccgagg aacctcgatc aagtcggctt gctgccgttt ttacgtggct cccttgtaag
     2701 gcgtccgaaa tgccggggcg ggaaacgctc cggcatttcg ttcaagaaaa aacctagaat
     2761 cccactgatc ccacgcctgt tccgggcgtg ctcgcgcccc tcgattcgtc tgaacccatc
     2821 ccggcggaac cggaaacccg gggcttggag taatcacccg acaaccctat attgcgggat
     2881 gtcccttttt cggtggaagc gccggcgcct tccggcatag caccggtcgt cgctcgagaa
     2941 ccgcccgccg aactgccctg cccggctaat gccgggcctt gcgctgattg cgccccgctc
     3001 attcccgatt ggacaccccc tgcaggcccg gtcgcctgtg catgaacaaa actcacgcat
     3061 gtaagtaaac cggcaagcag cacgggtcgt agcacgctta ctgaacacat atagctcata
     3121 tgccctcctt tattcggcag gattgcctat tcgaagaggt atcgtacgcc tggagataaa
     3181 tttatcggta cgctgccgca cccttgtcca atcgcgcttc acgaagactt attcagaggt
     3241 tccctggatg ctcgcgagca gctccttgag ggtcttgaga tccaccggtt tggtgagatg
     3301 atggttaaaa ccgctggcct tcgattgacg gcggtcttcc tcctgtcccc agcccgtcag
     3361 ggcaaccagc gttatatcgt tgaactccgg ccggcttcga atatgccgtg ccacctcgtg
     3421 accgctcatg cccggcatcc cgatatccat caggatcacg ttcggcttga aatcggggat
     3481 ggccgaaagc gcgtctcgac cgctgttcac cgcccacgcg ttcatgccca tggatgcgag
     3541 aagcatctgg aggacttctg ctgcatcctg attatcgtcc accacgagca agcgcaagcc
     3601 ctccggctcg tgggaaaccg tgtcgggatg cgggcttgta tcagtatttg gcgcttgcgc
     3661 caggggcaaa cgaaccacga attcgctgcc ctgatctttc ccattactca tggcttcgac
     3721 agtgcctcca tgcattgaaa caaggcggtg gaccaaattc agtccaattc ctaatccttc
     3781 ctgtgtcccg attcccctac ggcgctcctg ggaaaacatg tcgaacaggc gaggcagcat
     3841 acccggcagg atgcctattc cgtcgtcacg caccgagaca atcgcataac tcccctcctc
     3901 ccgggcagac agccagatat gcccatcatg cggcgtatac cgggccgcgt tattgagcag
     3961 attggtaaat acctgcccaa ggcgcacgct atcggcatag accattagag gcaccttgag
     4021 aatggatatg ctcaactggt gttttttttc cgcgatcaca ggctggcaag cctcagtaac
     4081 atcgcccagt accgcagcca ggtcagtggg agcacatcgc aattcgactt ttccactcgt
     4141 aatacgcgag acttccagca gatcatccac cagccgtacc atgtagttga cctgtcggct
     4201 gatgatggag agcagatggg gaactgcggc tgagttatca gaatgctcca gcagcgtcag
     4261 cgcgcttgcg accggtgcga gtggattgcg cagctcatgc gcaagcgtag ccaaaaactc
     4321 gtccttcagg cggccggctt ccctcaacgc gttttccgca ttaaccgtat cggtgatatc
     4381 gatcgatacc cccacatgcc ccgcgtaatc cccttcaaca ccgaaccatg gtaaagcata
     4441 cgacttgagc cagcgaagcg taccgtccgg cttccggacc ctgactcttt tctggaagcg
     4501 cgagcgatcg catacggcct gctcaaacgc tgcaatatag tccgtcccgt cctctggatg
     4561 caagattgaa cgccaccccc agggtatcaa ctcttcgacg cttcgcccca tcaattcgtt
     4621 gcagcgctgg ttgaggtaaa caagacctcc gcgctcatca gtctgccagg tcaatgcggg
     4681 cgaagcctcc gccatcgtgc ggaatcttgc ctcgctctcc cgcaaccgct gctcggctcg
     4741 cttacgccgg gttatctccg tgacatagcc cacactttcg agggcgttga gctgcttcgc
     4801 agtaaatgcc gcccaccagg ttgagccatt ctcgcggacg aattcctgct cgtaatagcc
     4861 cgtgtgccca cctgtctcgc atccacctgt ggcgcgcaaa agcagttcgg cccattccgg
     4921 cggggtgtgg acgtcaaggc gcagcaggcc ctcggccctt tcagttccgc tgaccccgct
     4981 cattgtcagg aaagcttcgt tggcctctgt aatctcgccc ttggtatcga agaaaatgac
     5041 gccgaccgtt tccacctcaa gcgcccggcg gaaatgctcc tctctcttgc acagcgcctc
     5101 ctcggctttc ttacgctttt gaacatgcgc tgtcacgtcg atgccgtgcg ccaggacacg
     5161 ctcgcagacc ccgtcctcat ccagcagagg caggtaaaca agatcaatga aacgctcctc
     5221 cggcgcaatg ccaggagctc gagcgagcga catgcgcatt tcagaaccta tgaatggttt
     5281 tcccgtcgcc ataacgtggg cgaggtgctc atggcaccca tctggagcag attccggaat
     5341 gacagcagaa taggagcgcc cgatcaaatc atcctgtcca atcaataagt agcacgcatt
     5401 attggcaaat tcaaaagtga gatcaggccc ccgcagcacg aatatgaatg acggagcgac
     5461 agaaaatact tcttcaagca actgaatcct gttcgcttgc gccacttccc ggagacgaac
     5521 ccgctcagct tctctctcgc gtgcctggat cacttccgtc gtctcaatga ctgtcacaag
     5581 cactccccct ggactgccgc ccttcgcacc gatctcttcc ggcattgtgc ctggaacagg
     5641 gctgtaagta agcttgaaga acgattcact gggaactcca ttacggttca ggacaaacct
     5701 ttgctcgtcg aatgtcagtg actcggctcg ctgcatgacg gccgcgcacg cgggttccac
     5761 gaaatcccac acctccggcc agcattcgct taccttctgg cccagtcccc gaggatgctt
     5821 gctgcctaca agattgcgaa agccgtcatt gtagatctgc acgaggtttt ctccccacag
     5881 caacagggcg gcaaaaggca accggagcat gatattgacc gcaagctgaa gctggggcgg
     5941 ccagtcctga gcagggccca gacccgagtc cgtccagtca tgtgtaaaaa tcaactcgcc
     6001 catttcagtc cctcctagcc atccgggaac gctggaacgc tctgttgccg cttgatccat
     6061 gcctctgatt acttcccgtt taatcacgag tgacccctgg catatcaact cttcatcgag
     6121 atgcctgtta gcattaatga atcaatattt tcgccatccg gttcacccag tcaattttag
     6181 ttcttgtcac ggcgcccttt tcgaactcta tcacagtcgg gcgtggcccc aagggtgcag
     6241 ggagattcgt aacatctgtg cgagaccgaa tagaagaacg attcgcgaag gaattatcgt
     6301 gacgttaaaa agtcgaatga acgtcaggca caggctctgc aagaagccct tgcggggaca
     6361 ttggtattcg cagccttcta aaatcgagag catttgggtc atgattaatt ttagatcgcg
     6421 gatgagggaa acaaaaaaaa tctctgcaat ccgctccgtc gtattcccac tggtatcgct
     6481 tcatgtctcg ggtaccttgg cgagaagcag cacggccagc aggagttcgc caactgcgcc
     6541 ggtgtggcca tga
//
LOCUS       DASSHJ010000132         5486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_723459, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000132 DASSHJ010000000
VERSION     DASSHJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723459"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2667
                     /locus_tag="VFI43_05220"
     CDS             <1..2667
                     /locus_tag="VFI43_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HET7061560.1"
                     /translation="VAFQPASPPSASQLLFLTTLLVRVLAVAQGKKTASHIAVLRLIA
                     VLSGSASQHFRNSLGQISFHPFNAWISACNYPRSLLAALHGILIGLGTISLALSFNVV
                     AESTVPTAEAAQTFDILPSTLEEALKSFTQQTGVKVSFDPVEVKGLMTQGLRGNFTVD
                     AGLAQLLAGTGLEASPEANGYAVKKAPGSPPPTGAAPPGGVTILPSVVVSATKGRDYT
                     VAESVTATRTNTLLRDVPQSITVVTQDLIKDQAMLSMADVVRYVPGVQMAQGEGNADT
                     PIFRGNQSTADFYVDGIRDDVEYIRDLYNSERVETLKGPNGMIFGRGGSGGVINRVTK
                     KAGWDPIRSFSFQAGSFGTKRVLMDVGQGINEVAAFRLNAMYENSGSYRDGVTIKRHG
                     INPTLTLRPTDRTNIVMGGEYFRDDRTADRGIPSFMGRPVHTDVSTFFGDPKRSHAGA
                     TVLSLYSIIEHAFDNGIRFRNRTRYAVYDKFYQNIFPFGVDPNNPDGTVFDVGAYSNS
                     TKRSNVFNQSDLMYTLNTGPLEHRLLAGIEVGQQITNNLRKTGYFGQDQCATDHVDDP
                     EDDNLPSLRPVSLSNPRITAPVDFRLDACGVTNHVVTNITGLYIQDQLKWSKFQAILG
                     LRYDNFELKTHNNRTGEDLRKTNGLLSPRAGLIYKPIEPLSIYWNYSLAYVPRAGDQL
                     NALEVTNQALEPERFINLELGAKWDIRPNLALTAAAYRLDRTNVAIPDPLNPVRLLLV
                     NGQRSEGIELGVSGRVTAAWGIMGGYAYQDSRIRRSGAQEDAVAGAILAQVPHHTFSL
                     WNRYDITSWLGFALGVIHRSNMFASVDNTVVLPSYTRLDAAMYARLDRRLRFQVNVEN
                     MLNTHYFLAASNNNNITPGSPVAIRGMMTLDF"
     gene            2698..4011
                     /locus_tag="VFI43_05225"
     CDS             2698..4011
                     /locus_tag="VFI43_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HET7061561.1"
                     /translation="MNEINSCKREGSHKSKSWITTGALLTFLAASIPAYAAGYKQTNL
                     VASNDSYGASIVDPTLINAWGIAIRPAGFGGHFWVESNGAGTSNQFIGDVGGVPLFSD
                     DLRLVTVPGPATGKAASIGTPTGVVFNPGVQFKVTQGSLTEPAKFIFVTDTGTISAWT
                     ERKNPDGSFDRPAFAKLVVNRSQAGAHYFGVALGPGNDRIHVANFGPSAGIQSFDGTF
                     HEINGPGAFANPFSTSATPHPGDYAPFNIQALTGRDGTNLFVPYAKTQEDPARPGQIL
                     GGEEISGAGLGRLAEFDSSGELLHTWDDRGLLNAPWGVAFAPERFGDYSGKLLVGNFG
                     DGTIVAFDPATHKAIDYLRDEQGHVISIDGLWGLQFGNGASLGRADALYFAAGPKGET
                     DGLFGRLVAAPIPEPETWALFTAGFILLGSVARRRVRSSLGEARR"
     gene            4283..4488
                     /locus_tag="VFI43_05230"
                     /pseudo
     CDS             4283..4488
                     /locus_tag="VFI43_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
     gene            complement(4844..>5486)
                     /locus_tag="VFI43_05235"
     CDS             complement(4844..>5486)
                     /locus_tag="VFI43_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7061562.1"
                     /translation="QFKQELNMPASILTSYTGDAAAFQSSQASQVILIVAALLVIYIL
                     LGVLYESYIHPITILSGLPSAAVGALAMLWLFKIELSIIAMIGILMLIGIVKKNAIMM
                     IDFALDAQRNEGMQPDEAIRTACLLRFRPIMMTTLAALMGALPIALGLGAGAEVRQPL
                     GLAVVGGLLFSQVITLFITPVIYLYLDRFSGKGPLKLDQETERMKLATQVPQG"
BASE COUNT         1264 a         1506 c         1450 g         1266 t
ORIGIN      
        1 gtggcctttc agccagccag ccccccctcg gcaagccagc tacttttttt aactactcta
       61 cttgtcaggg tattggctgt ggcacagggt aaaaaaaccg cctctcatat agcggttctt
      121 cggcttatcg ctgtcctttc aggctcggca tctcaacatt ttcgcaattc tctcggtcag
      181 atctctttcc acccgtttaa cgcctggata tcggcttgca actatccgag gtcactcctc
      241 gcagcattgc atggcatcct gatcggcttg ggaacaatct ccctggcgtt gtctttcaat
      301 gttgttgctg aatccaccgt tcctacagcc gaggctgcac agaccttcga tattcttccc
      361 agcaccctgg aagaagcact gaagagtttt acgcaacaaa cgggtgtcaa ggtctccttt
      421 gatcctgtcg aggtaaaagg actgatgact caaggactac gaggaaactt tacggttgat
      481 gcagggctgg ctcaactcct ggctggaacc gggcttgaag catcacccga ggcaaatggc
      541 tatgcagtga agaaagctcc tggctcccct cccccaaccg gtgctgctcc tcctggcgga
      601 gtgaccatcc ttccttcagt tgtggtttct gccaccaagg gacgtgatta cactgtagcc
      661 gaaagcgtga cggcgacccg aacaaacaca cttttgcgcg atgtgcccca atccatcacg
      721 gttgtaacac aggatctgat caaggatcag gccatgctga gcatggcgga cgttgtgcgc
      781 tatgttcccg gcgtgcagat ggcgcaggga gaaggcaatg ccgacacacc gatcttcagg
      841 ggcaaccagt caaccgcgga tttttatgta gacggcattc gtgatgatgt cgagtatatc
      901 cgggaccttt ataactctga gcgcgtggaa accttgaagg gcccgaatgg catgatcttt
      961 ggccggggtg gttcgggtgg cgtcatcaac cgggtcacca agaaggccgg ctgggacccc
     1021 atccgctcct tctcgttcca ggcggggtcg ttcggtacaa aaagagtgct catggatgtc
     1081 ggccagggca ttaatgaagt ggccgctttt cgcctcaatg ccatgtatga aaattctggc
     1141 agctatcgag atggcgtcac aataaagcgc cacggcatca atccgacgtt gacgcttagg
     1201 ccaacggacc gcaccaacat tgtaatgggc ggggaatatt tccgggatga tcgcaccgct
     1261 gaccgcggca tcccgtcgtt tatgggccgg ccagtccata ctgatgtttc cactttcttc
     1321 ggcgacccca agcgcagtca cgcgggcgcc acagttctta gcttgtattc cattatcgag
     1381 catgcattcg acaacgggat ccggtttcgt aatcgcacgc gctatgcggt ttacgacaag
     1441 ttttaccaga acatctttcc tttcggtgta gaccccaaca accctgacgg aaccgtgttc
     1501 gacgttggcg catacagtaa ctccaccaag cgcagtaatg ttttcaacca gagcgacctg
     1561 atgtacacct tgaacacggg gccgctcgag caccggctgc tggctggtat cgaagtcggc
     1621 cagcaaataa caaacaatct tcgcaagacc ggctatttcg ggcaagacca gtgcgctacc
     1681 gatcacgtgg atgaccccga ggacgataac ctccccagtc tgcgcccggt atcgctcagc
     1741 aatccgagaa taactgctcc ggtcgatttc aggctggatg cctgtggcgt aaccaaccac
     1801 gtcgttacaa atataaccgg gctctatatt caggatcagc tcaaatggtc caaatttcag
     1861 gcgatattgg gtttgcgcta cgataatttt gaattaaaaa cgcataacaa tcgcaccggc
     1921 gaggatctca ggaagacaaa tgggttgctc tcgccacgtg ccggcctgat ttataaaccg
     1981 atcgaaccgt tatccatcta ctggaactat agcctggcat acgtcccacg cgccggcgat
     2041 cagctgaacg cattggaggt aaccaaccag gcgcttgagc cggaacgttt catcaacctt
     2101 gaactgggcg caaagtggga cattcgcccc aatctggcgt tgactgccgc tgcataccga
     2161 ctggatcgta ccaatgtagc cattcccgac ccgcttaatc ccgttcggtt actgctcgtc
     2221 aatggccagc gcagcgaggg tatagagctc ggggtgagcg gacgcgtgac cgcggcatgg
     2281 ggcatcatgg gaggatatgc gtatcaggat agccgcatca gacgctccgg ggcgcaggaa
     2341 gacgcagtag ccggtgctat tctggctcag gtgccccatc atacattttc cctctggaat
     2401 cgctatgaca tcacatcctg gctgggtttt gcgctcggcg tcatccatcg cagcaacatg
     2461 ttcgcctcag tggacaatac cgttgtccta ccgagctata cccggcttga cgcagcaatg
     2521 tacgcgcggc tggaccggcg tctacgtttc caggtgaatg tcgagaacat gctcaatacg
     2581 cactacttcc tcgccgccag caataacaac aacattacgc cgggctcccc tgttgcaatc
     2641 agaggaatga tgacgctgga tttttgagac gctctgtaaa atgttatcgg aggaaaaatg
     2701 aacgaaatca actcgtgcaa aagagagggt tcgcacaaaa gcaaatcatg gataactacc
     2761 ggcgctttgc tcacattttt ggccgcttca attccggctt atgccgcagg atataagcag
     2821 acgaacctgg tggccagtaa cgattcctac ggcgcgagca tagttgaccc aacgctcatc
     2881 aatgcatggg gcattgcaat tcggccagcg ggtttcggag ggcatttctg ggtggagagc
     2941 aatggagcag gaacgtcgaa tcagttcatc ggggatgtcg gaggtgttcc gctgttctcg
     3001 gacgatctcc ggctggtaac ggtacctggg ccggcgaccg gcaaggcggc ctcgatcggc
     3061 actccgaccg gggtagtatt caatccaggc gtgcaattca aggttacgca gggatcgctt
     3121 accgaacccg caaagtttat ctttgtcact gacactggca cgatttcagc atggaccgag
     3181 cgcaagaacc cggacgggag ctttgaccgg ccggcattcg ccaagcttgt cgtcaaccgc
     3241 tcgcaggcag gtgcgcatta tttcggtgtc gcgttgggtc ctggcaacga ccggatccac
     3301 gtcgcaaact ttgggcccag cgctggcatc caaagttttg acggaacatt ccatgagatt
     3361 aacggccctg gcgccttcgc caatccgttc tcgacttctg caactcccca tccgggcgac
     3421 tatgcgccat tcaatatcca ggcactgaca ggtcgggatg gaacaaatct gtttgtgccc
     3481 tatgccaaaa cgcaggaaga cccggcgagg ccaggccaaa tattgggtgg cgaagaaatc
     3541 agcggcgcag gcttgggaag gctcgcagag ttcgactcat ccggcgaatt gctgcatacc
     3601 tgggatgacc gtggcttgct gaacgcacct tggggcgtcg cctttgctcc cgaacgcttt
     3661 ggcgactata gcggtaagct gctcgtaggg aatttcggcg acggcacgat cgtcgcattc
     3721 gatcctgcga cccataaagc aatcgactac ttgcgggatg agcaaggaca cgtcatttcc
     3781 atcgatggac tatggggact tcagttcggc aatggcgcga gcctcggccg cgccgatgca
     3841 ttgtatttcg cggcaggccc gaagggcgag accgacggcc tcttcggcag gcttgttgcc
     3901 gcgccgattc ctgaacctga aacttgggcg ctattcactg ctgggttcat tcttcttggt
     3961 tcggttgcga ggcggcgcgt gagaagctct ttaggcgaag caagacgata ggaacatgtg
     4021 gcatatcaaa agatattcgc gtcggtgtac ctttaccata tcacgatctc ctttttgata
     4081 atctttaata tggacccctc gttatttctc agaatacttt ttgattacat ttaattagat
     4141 gcaattccaa atgcaaccat cagccgtttg gcctttcagc caagggccac aaagatttcg
     4201 gccttactgg acacgagtgg aagcgctgat caaggcgacg acgaacgtcg gttatccctc
     4261 caccgttcga aagtgactcg ctatgagcgc catcgttctt gaatctgtac tggtctgtcc
     4321 gcattgcggc tttgccaggc aagaaattat gctacggacg cctgtcagtt ctactacgaa
     4381 tgtcacaact gcaaaacgtt gctgcgcccc aattcgggcg attgctgcgt attctgctcg
     4441 ttcggctcgg tgaagtgccc gccagagcag gacgggtgct gcgggtaggt ctcccatacg
     4501 atttttttcg tttcgtgagc cggccaagaa gcggggttgc tatgaaaggc aataaacttg
     4561 ggatagtttg gtataggaga aactgttacc aaaactttct atttaaatgc tcggagtttg
     4621 tctattcctt gttgaatttt cctattagtt ccggctccat gacttgcaca ccgttggcta
     4681 ctaaccctcc ctaacagacc cctccagcgg tccgcgctgt atacataacg tgcattatac
     4741 gtatcccctc aagaacctga aggctttgaa atatcccgac ctggttatct cggaggggaa
     4801 gattttggtc aataatttcc aaaactaaaa agaggcttag cccttagcct tgcgggacct
     4861 gagtcgccaa cttcatgcgt tccgtttcct gatccagctt tagcggtccc ttcccggaaa
     4921 aacgatccag gtagagatag atcacaggcg tgatgaaaag cgtgatgacc tgggaaaaca
     4981 gtagtccgcc caccacagca aggcccaatg gctgtcgcac ttcggcgcct gctcccaatc
     5041 ccagcgcgat gggaagtgcg cccatcagtg ctgccagggt ggtcatcatg atgggccgaa
     5101 aacgcagcaa acatgcagtg cgaatcgctt cgtccggctg catgccttcg ttgcgctgcg
     5161 cgtcgagggc aaagtctatc atcatgatgg catttttttt cacgattccg atcagcatca
     5221 ggatgccaat catggcgatg atcgataact ctatcttgaa cagccacagc atggccagcg
     5281 cgccgactgc cgcggagggc aaccccgaaa ggatggtaat agggtgaata tagctttcat
     5341 aaagaacacc cagcaatata tagatcacca gcagagcggc aacaatcagg atgacctggc
     5401 tggcctgaga agactggaac gcagcagcat cccccgtata gctggtcagg atactcgcag
     5461 gcatattcaa ctcctgcttg aattgc
//
LOCUS       DASSHJ010000133         5527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1442796,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000133 DASSHJ010000000
VERSION     DASSHJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442796"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFI43_05240"
     CDS             <1..1159
                     /locus_tag="VFI43_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7061563.1"
                     /translation="IEHDLVFAGLIGMIDPPRAEAGEAVAHAKTAGLRPIMITGDHPI
                     TAGVIALQLGITTEARAVSGAELEKMTDEDLDKMVRDVSVYARVSPEHKLRIVKALQR
                     GGAVVAMTGDGVNDAPALKTADIGVAMGITGTDVSKQAADMVLADDNFATIVAAVEEG
                     RAIFSNIRKFLRYLLSSNIGEVLTMFFGVVLADLIGLKAGEGTVVLPVLATQLLWINL
                     VTDGAPALALGIDPPNARVMSRPPRPHGESVITPKMWFGILLVGAIMAAGTLLVLDAS
                     LPGGLIEGTGSMRYAQTMAFTTLVLFSVFTVFNSRSDEQSAFVGMFSNKSLWGSLLLS
                     ILLQVAVVYTPFLQQAFSTVSLSLQDWLFCAAVASSVLWLRELSKLVGRAV"
     gene            1389..2057
                     /locus_tag="VFI43_05245"
     CDS             1389..2057
                     /locus_tag="VFI43_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061564.1"
                     /translation="MKILAGFINLLTLVMAAQVVQAQTVYKTIDDFNTGLQTIDPLPS
                     GRRVDFQSGSMIGGLRQIAYQVGDIEPATSLGRKGVYAINKGHLIVEEGVHVASRLEI
                     SYGIGRDNSIVPLNLNLSRVLTNGQFALHFHSLDMINQVDVILQVVNQAGEMFQFGKQ
                     VIPPVVGKFDIYLPIADFRSNSTGNPPLESDLTSISYITLIFQEAGNGGTDYAVDSFD
                     ISTQ"
     gene            2436..2957
                     /locus_tag="VFI43_05250"
     CDS             2436..2957
                     /locus_tag="VFI43_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET7061565.1"
                     /translation="MAELIAVGFQDPEEADRVLLRLSKLKKEYLIDLEDAVVVVRDES
                     GKVHLKQSINLISAGATSGFLSGSLWGTLVGLLFLNPLVGFVVGGAFGAGAGALSGSL
                     ADYGINDNFIKSIGETIPPNSSALFILVRKAQPERVLAELSGLKGKVLRTSLSPEQEK
                     KLQEALEGSSSGG"
     gene            complement(3094..3540)
                     /locus_tag="VFI43_05255"
     CDS             complement(3094..3540)
                     /locus_tag="VFI43_05255"
                     /inference="COORDINATES: protein motif:HMM:NF022159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HET7061566.1"
                     /translation="MNNFHYRSWITASSAVTALFCGSLSAQGLGQLKDIAGSSGLGNA
                     ASSLGSLSSIAAGSPGNAAGIITFCMKNNYLGGEGAASIKDKLLGKTANNGEESAETN
                     PDYISGTKGIVCCGKGPSVDLSMSTLKSSVVKKACQKLLEQGKSML"
     gene            4417..4839
                     /locus_tag="VFI43_05260"
     CDS             4417..4839
                     /locus_tag="VFI43_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061567.1"
                     /translation="MTNLDKGNDEAINSADRVRRATAADVNAKIDRQIDRNISYYSGA
                     PRNAILTRVRELDSEWDIERVLEIQCSSLALAGLVLGISSSRRWLAVPAVVLSFLLHH
                     GLRGWCPPVPLLRGIGVRTRGEIDREKYALKEYLEKSA"
     gene            complement(5111..>5527)
                     /locus_tag="VFI43_05265"
     CDS             complement(5111..>5527)
                     /locus_tag="VFI43_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7061568.1"
                     /translation="GAGSQLAAPVLARRIGLLNTMVFTHIPANICLIFAALVPNIELA
                     LGLLLLRSALSQMDVPPRTAYVMAVVTPAERAAAASFTAVPRSLASAGSPALAGAMLA
                     GGLLSGPLIACGVLKIVYDVALLASFRGLKVPLDRQ"
BASE COUNT         1253 a         1533 c         1475 g         1266 t
ORIGIN      
        1 catcgagcat gacctcgtat tcgctgggtt gatcggtatg atcgatccgc cccgtgctga
       61 agccggggag gccgtggcgc atgcgaaaac agcgggacta cggccgatca tgataacagg
      121 cgatcatccc atcactgccg gcgtcattgc gctccagcta gggatcacca cagaagcgcg
      181 agcggttagc ggcgctgaac tggagaaaat gaccgacgaa gacctggata aaatggtaag
      241 ggatgtttcg gtttatgccc gcgtcagccc ggagcataag ctgcgcatcg tgaaagcact
      301 gcagcgtggc ggggcggtgg tggcaatgac tggggacggg gtcaatgatg cccctgcgct
      361 gaagacagcc gatatcggcg tggcaatggg cattacagga acagatgtct ccaagcaagc
      421 ggctgacatg gtacttgccg acgacaattt cgccaccatt gtggcagcgg tcgaagaagg
      481 ccgcgccatc ttctccaaca tccgcaagtt cctgcgctac ctgctttcat caaacatcgg
      541 cgaagtcctt accatgttct tcggcgtggt gctggctgac ctcattggcc tcaaagcggg
      601 agagggaaca gtcgtactgc ccgtgctggc gacgcaactg ctctggatca acctcgtgac
      661 cgatggcgcc cccgcgctcg cactgggcat agatcccccg aatgcccgcg tgatgagtcg
      721 accaccacgg ccgcacggtg agtccgtaat caccccaaaa atgtggtttg gcattctcct
      781 ggtcggcgcc atcatggctg ccggcacact tctagttctg gacgccagcc tgccgggcgg
      841 cttgatagaa ggaacaggca gcatgcgcta tgcgcagacc atggcgttta ctacactagt
      901 gctgttttcg gtatttaccg ttttcaattc gcgttccgac gagcagagcg catttgtcgg
      961 aatgttttcc aataagtcgc tgtggggctc gctcctgctc tcgatcctgc tacaggttgc
     1021 cgtggtttac acacccttcc tgcagcaagc gttctcgacc gtcagcctgt ccctgcagga
     1081 ctggctgttc tgcgctgcgg tcgcaagctc cgtactttgg ctgcgcgagt tgagcaagct
     1141 ggttggccgc gccgtgtaga caagctcagt cgtccctggt ccaaagtttt accaaaattt
     1201 taccgtttta ttacgacttt cccgtgaggc cggcgtatgg tcaatcatgt ccacttattt
     1261 gatgggacgt ctaacaagcc gctaacgatg aaccccaacc gtattaggaa caaggattaa
     1321 caccttaatg aattaagacc ataccaaaaa cacggagatg ggtttgtgct gatgaaaagg
     1381 agaaattcat gaaaatactt gctggcttca tcaacctttt gactctggta atggctgccc
     1441 aggtagtgca ggcgcagact gtgtataaaa caatagatga tttcaatact ggattacaga
     1501 caatagatcc ccttccatcc ggaaggcgtg tcgatttcca gtctggctcg atgattggtg
     1561 gtttacgtca aatagcgtac caagtgggcg atatcgaacc cgccacttct ttaggcagga
     1621 aaggggttta cgcaatcaac aaaggccacc tgatcgtcga ggagggtgtc cacgttgcga
     1681 gccggcttga gatttcatat ggaatcggca gggacaatag catcgtccca ctgaacctca
     1741 acttgagcag agtcctgaca aatggccaat tcgcgctgca tttccattcg ctcgacatga
     1801 taaatcaggt tgacgttatt cttcaggtgg tcaaccaagc cggcgagatg tttcaattcg
     1861 gcaagcaggt cattcctccg gtggtcggaa agttcgatat ctatctcccc attgccgact
     1921 ttcgctcgaa ttcaactgga aatccgcccc tggaaagtga cttgacatct atttcctaca
     1981 tcacgcttat ctttcaggag gccggaaacg gcgggactga ttacgccgtt gattcattcg
     2041 atatttctac ccaatgagca cacagcattc ccccctgctg ggttactccc cttcgtctgg
     2101 atgcttgagc tgagctagaa aagcaatgag ctggagcaga acccattacg tgctttcaat
     2161 tgggttacgc ttcgccaact cattctacag atgctctaat ccatcctctc cgcttctgat
     2221 ccgtctcgct gctgtttttg catgcagcct cttgccaccg ttttgtctca acgccttgtc
     2281 tctgtgcgac ggcgaacgga aaaacgattg agcgaaagct atcctcccgt ttcaccttcg
     2341 ttggctcatc cacaagctga gggttgcccc ttcagaacca agataatcct tttctttgga
     2401 atgtctcgca agcatcactt tgacaggaga acaatatggc cgaattaatc gcagtagggt
     2461 ttcaggaccc tgaggaagcc gatcgcgttt tgctgcggct tagcaagtta aaaaaggaat
     2521 acctgatcga cctcgaggat gctgtcgtag ttgtacgtga cgaaagcggc aaagtccatc
     2581 tcaagcaaag cattaacctg atctctgcgg gagcaacttc cggatttctc tccggaagcc
     2641 tctggggcac gcttgtcggc ttgttattcc tcaatcccct tgtcggattc gtggttggcg
     2701 gcgccttcgg ggctggcgca ggggcgttgt ccggctcgct cgccgattac ggcatcaacg
     2761 ataatttcat caaatccatt ggggaaacga ttccccccaa ctcgtcggct cttttcatcc
     2821 tcgttcgcaa ggcacagcca gagagggtcc tggcggaact ctccggcctc aaaggcaagg
     2881 tcctgcgaac ttcgttatct ccggagcagg agaaaaaact ccaggaagcg ctggaaggat
     2941 cctcttcagg tggttagcaa cccgcccctg cgtgatgttt tgcaacattt cctgacagtt
     3001 gcgcaaaccg cagtgcgcat atgaaagcct cagcctgcga tatccggggt ggcaagacca
     3061 cgcgaaaacc tcgaatgctt ggaatgagca ggatcaaagc atcgacttgc cttgctcgag
     3121 cagtttttga caggccttct tgaccactga agacttcagc gtggacatgc tcaaatccac
     3181 gctggggccc ttgccgcaac agacaattcc cttcgtgccg ctgatatagt cagggttcgt
     3241 ctcggccgat tcctcgccgt tattagcggt cttcccaagt agcttatcct tgatggaagc
     3301 agctccttcc ccgcccaggt aattgttctt catgcagaac gtgatgatgc cggctgcatt
     3361 gcccgggctt cccgccgcga tggatgacag cgatcccaac gacgatgcgg cattacccag
     3421 gccactactg cctgcgatat cttttagctg acccagccct tgtgcggaaa gggaaccgca
     3481 aaacagcgcg gtgaccgcgc tgcttgccgt tatccaggaa cgataatgaa aattattcat
     3541 actttcctcc agttgttgga cgaggccttg ccattgacat tgttgctgga aaacctcgtt
     3601 ataagataga aactggaatt ccagaaatag ttttcacatc cacccgagcg catcccgcaa
     3661 ccgctgcaac tggttacagc gcggccccgc cgactcttgc atctctgcgg acgtcgaaaa
     3721 ttattagacc gcccctcggc gcagtgcaga acataattca cgtcgttacg ggggaagtcg
     3781 gcagattcaa ccagaactgc ttttcattaa tcataggtct gatagccact tgctggggga
     3841 aaaaatactg tttataaggc aattttcctc tttgcagcgc tcttcggaga gcatttcccc
     3901 tctctccgaa caccggcatt ccgcccgcct gtcgagcccg acgcaacttc gaaatgggat
     3961 ggcaagccgt ttcccaatca catgttcttg cttaagcgta gggcagtctc caactcacct
     4021 atatgtgcgc tgccgagcag aaaaccacag cgtttaaggg gacaatgggt gcaagtgaac
     4081 ggtggatgag tcaccgttcg cgctaaaact ttaacggagg catgcgccat gaacagcatt
     4141 atctggtggg ttggctttat cgttatcctt cttgcgatcc tcagtttcct ggggctgcgt
     4201 taagcgggag cattgctccc attgcctctc gcaaaggcgg aaggtgctcg tttggaagga
     4261 atggtctacg cgggcgcctt ctttctcaag aaatctggga aattgcacgc aacagcaaaa
     4321 cgctttaaac attcgcctca tgtaccggag tacgggcgta tcggctcctg ggacgacggt
     4381 tgcgcctgtt ttcagcatgg attgtaaaca caggaaatga cgaacctgga taaaggcaat
     4441 gacgaagcta taaatagcgc agaccgggtc aggcgcgcga ctgctgcgga cgtcaatgcg
     4501 aaaatcgaca ggcagatcga ccgcaatatc tcgtattaca gcggtgcccc gcgaaacgcc
     4561 atccttaccc gcgtaagaga gcttgatagc gaatgggata tcgagcgagt gctggaaatc
     4621 cagtgctcgt cacttgcgct cgcagggctt gtactgggga tatcctccag ccggcggtgg
     4681 ctcgccgtcc ccgcagtcgt cctttcattc ctgcttcatc acggcctgcg aggctggtgc
     4741 ccgcccgtac ccctactgcg cgggatcggt gttcgcaccc gcggcgaaat cgaccgggaa
     4801 aaatacgcac ttaaagagta cctcgaaaag agtgcctgaa accggacggc gccagattca
     4861 gatacttgga tagtgcggac gcaggcataa ggtttggcag atttttcgag gcgttcaaca
     4921 tccggccagc gattgggacc agctagggtc ggtcgacaag ttttttcagc tggctacggg
     4981 ccttcgcccc tcggaatgtg gtatgcgact gatggccttt tgctagcctg tcctgacgtt
     5041 gagccagctc agcaacagga caggtgaagg cagaaaccca tcaggaatcg tgaagctatg
     5101 aaaggcaaac ttattgccga tcgagtggaa ccttcagtcc cctgaaagac gccagcaggg
     5161 ccacatcgta gacaattttc aggacgccgc aagcaatcaa ggggccgctc agcagtccgc
     5221 cggccaacat cgcgcccgcc agggctgggc tgcccgccga agccaggctt cgtggcacag
     5281 cagtgaaact cgccgccgcc gcgcgctctg ccggcgtcac gactgccatc acgtaagccg
     5341 ttctcggcgg cacatccatc tgcgataagg cgctccgcag caacagcagg cccagcgcca
     5401 gttcgatgtt tgggacaagc gccgcaaaaa tgaggcaaat gtttgccggg atgtgggtga
     5461 ataccatcgt gttgagcagg ccaatgcgcc gtgcgagaac gggcgctgca agctgtgatc
     5521 ctgctcc
//
LOCUS       DASSHJ010000134         9263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_663683, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000134 DASSHJ010000000
VERSION     DASSHJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663683"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1609)
                     /locus_tag="VFI43_05270"
     CDS             complement(272..1609)
                     /locus_tag="VFI43_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HET7061569.1"
                     /translation="MLVLIFAVYFAAGIRFFDQAAPLPFTFPSLFPEAGTFTFAMITS
                     MAAMGMYQKDSRKSLKDTLFRLMPSMMLGFGIMTLVFYLLPDLYLGRGILGLVLLVAL
                     PGILLTRAGFFKWSSLGILESQAIVLGSGGKAKEFVDQAGKDPVHRGLKIVGFVPLPD
                     EELHVPSSAVLPRMASLMTLANQYGAGEIVIAIQERRGGCFPIQDLLECKLNGIKVTD
                     SAAFFERERGQIRVNSLYPSWLVFGGGFDQSLLRSGIKRTFDIAASCVLLAATFPLML
                     VTALCIFIEDGASVFYRQERVGKGGKTFMVLKFRSMYKDAEKGGKPQWAATDDPRITR
                     VGRIIRKLRIDELPQVFNVLAGDMSFVGPRPERPYFVDQLCAEVPFYNVRHSIKPGIT
                     GWAQVRYQYGASVQDAIEKLQYDLYYVKNHSLFLDIIILIDTIEVVLLRKGGR"
     gene            complement(2197..2670)
                     /locus_tag="VFI43_05275"
     CDS             complement(2197..2670)
                     /locus_tag="VFI43_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7061570.1"
                     /translation="MKLSLETKFIILSQTVTEEIEWELFRIGVRGCCRSDIEAEQLSS
                     VVEAVGRGELWIRRSLSWRLLNELVTMTREQNRIKRTVSQLLSNLTRREYEIATLVGN
                     GESNKQIARRLAITERTVKAHLTEVFRKLDVADRLKLALIVKGSVSSSTTTQAEH"
     gene            complement(2846..3367)
                     /locus_tag="VFI43_05280"
     CDS             complement(2846..3367)
                     /locus_tag="VFI43_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HET7061571.1"
                     /translation="MQTVTVAIADRDPGRRKKLEQLLKGGAGIEVMANVMNASGKAHS
                     RKAAALNAACTEDIVLEVSRLRPRILFFNLDSFGSASLKMIEALNRECPDTFIVVLTE
                     GLIEEEQILRALANGARGYLTHEADRCYFLKAVRVVDQGEIWVSRKMLGRIMDKVCTD
                     ISLLGRGSLDFAR"
     gene            complement(3541..4365)
                     /locus_tag="VFI43_05285"
     CDS             complement(3541..4365)
                     /locus_tag="VFI43_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET7061572.1"
                     /translation="MLNIRQALPRLVRSYAKSLAMAALLFAPSLWASELVKTIERIKP
                     SIVGIGTLQRTRSPQLNFLGTGFVLDDGFHIVTNAHVVPEILDTVNKESYVVITGKGK
                     EPELRTATVAAVDREHDLALLKITGTPLPAMELGDAHRVREGQTLVFTGFPIGMVLGF
                     YPVTHRAMISAITPIILPAHNSRQLNSKMIGQLQKPRYAVFQLDGTAYPGSSGSPLFD
                     PETGLVYGVINMVFVKGTKESALSNPSGITYAIPGNYIRDLLKQGNEESSGDPFKK"
     gene            complement(4630..4887)
                     /locus_tag="VFI43_05290"
     CDS             complement(4630..4887)
                     /locus_tag="VFI43_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HET7061573.1"
                     /translation="MEIHVYDTYVKAKDGHTMHFDVFTPAKDHQKAIEYAKQWLASIG
                     EGDATVTSRECSFCHSQSAPANVEDAINKDGYFIYKMEGCR"
     gene            5190..6890
                     /locus_tag="VFI43_05295"
     CDS             5190..6890
                     /locus_tag="VFI43_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7061574.1"
                     /translation="MKLSKISLAAGAILGSSVAPLVFAIDLYVDTKTEQIYAKPGPGR
                     THIGSFVREDAGLKKERVSTGGTSETDKASDKTAEIMNDRTDAAELAAMRKDMELKAK
                     MREARLVSDVASLEERVKQAEKVHVELKEGAPHFASKDGNFTFALNGRMQVGSQYNFI
                     NEVLPATGSNLPNELNSGVTLRRARLGVEGTFFKIWDYKFEYDFSRGNGTVESGITDA
                     FVRLNVNKPFSLKVGSFKEPFSLEEASSNRFLTFIERHMAVNSFVDNPNAYKTGIGAN
                     YAEPRFQWGVALQTEPVGSWSAASTTVNTAGNNSRNNGAGDMGWEGIGRITGRPWMEN
                     ETKFLHVGVSAGHTVVNTQYQANGTINVGPNQTGGGGGMAFFAFPGTNVDRTNVLNTG
                     NLSIGNKGAPGSRQITSYDRYGAEGWLVHGPLSLQAEYLRTNINGVGYDNEHFTGYYG
                     FASYFLTGESKAYHIRNAAANRIKPRQNFQWNGPGWGAWEIAAGYDYINLNSGVIRGG
                     QADMVRFALNWYPHTHIKVQNNITYVLNINTEGSTIPRTAGFNNADLSSFLTQVQVDF
                     "
     gene            complement(6988..>9263)
                     /locus_tag="VFI43_05300"
     CDS             complement(6988..>9263)
                     /locus_tag="VFI43_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET7061575.1"
                     /translation="EAALRKLGIENHGTEIPYAAKIAEIQIKPVELNLNSAKRSNVAL
                     TLSEAAISREGEEQPVLSLPSLKVSQSSIDLAHQSVTLGDITLDRIHAFLRRESDGRL
                     NLAKLFTPASRSTAPRKAIAPEAKADKPWEVRFGSFKLTNAALRYEDVSLTNVSPMIV
                     QPLDLTVTDIDLRGAEPLKIALKAAVNKKGSLETNGTLAWAPLAVELDVDAKDVDLVP
                     LQGWAGDKMNALITSGATSFHGKIKADGAPIKVVLLGDGKFTNFSVLDKSATTDLMRW
                     RTLDIRGIEFVNEPLRANIEAVSIADFFAYVVITSKGDLNLTHIVRQEGNNESPVPPP
                     PPAETAEPGPTTASKTGAKTTAPADRKSTPVRISRISLQGGNINFQDQFIKPNYKANL
                     TGLAGRIGPLDPQKPGEIDIHGAVDKTAPLKISGKVNTFAKETYLDVTASAKGIDMPA
                     FSPYSAKYIGYTIEKGKLSVDVHYHVEKGELTAENNVFLDKLTFGEKVKSPDALSIPV
                     TLAVALLKNREGEINLHLPVTGSINDPQFSIGSVVVNAFVNLLSKAVTAPFALLGSVF
                     GRSGEELSEISFEPGRADLGPEAEKSLDVLSKALKDRPALELEITGEADPAYDPEALK
                     RVMLERKIKARKLLENVKKGETSGSLDDIALTPDEYAKYLTLAYKDEKFDKPKNVIGF
                     TKSLPVPEMEQLMLANIDAGESEMRDLAQARAENARDWLVEKGDISGDRIFLLQPKVD
                     AEGGNKKPGSKVEFSLR"
BASE COUNT         2221 a         2473 c         2359 g         2210 t
ORIGIN      
        1 aggcagtacg atcggacttg aacggctccg ccagcgagtc agtagcagcg ttgccaggaa
       61 aagatttgcc gcgcctgcca ttgtgtagct gattgccgca atgcccggtg ccatcacacg
      121 acgagaccgg caactacagc aagcgggaaa aaacaacgca gtcccgcggg agtcctcgac
      181 ccctcctttc cgagggggga actgtggaat ttcccagtag atgcagtgtg cttgaagaac
      241 acgtctgtca atgacgaggg gatcttccgc ctcatcgtcc acccttgcgt aacaggacga
      301 cttcgatcgt atcgatcagg ataatgatat cgaggaacaa gctatggttc ttcacgtagt
      361 aaagatcata ctgaagcttc tcgattgcat cctggaccga ggcgccgtat tggtagcgca
      421 cctgcgccca tccggtaatt cctggtttga tgctatgcct gacgttatag aacggaactt
      481 cggcacaaag ctgatctacg aaataaggcc gttcaggacg aggcccgacg aagctcatgt
      541 ctccagcgag gacattgaat acctgcggaa gctcatctat tctaagtttt cggattattc
      601 tccccactcg cgtgatccgg ggatcatccg ttgcggccca ctggggcttt ccgccttttt
      661 ccgcatcctt gtacatgctg cgaaacttca acaccatgaa ggtcttcccg ccctttccaa
      721 cgcgttcctg gcggtaaaag accgatgctc cgtcttcgat gaagatacac agggcagtaa
      781 cgagcatgag cggaaaagtt gccgccagga gcacgcagct tgccgcaata tcaaacgtcc
      841 gcttgatgcc ggatcgaaga aggctttgat cgaatccgcc gccaaaaacg agccaactcg
      901 ggtaaagact gtttacgcgt atctgccctc tctcgcgctc gaaaaaggca gctgaatcgg
      961 tcaccttgat gccgttcagc ttgcattcga gcaaatcctg gataggaaaa cagccgcccc
     1021 gccgctcctg gatggcgata acgatttctc ccgccccata ttgattagcc agagtcatca
     1081 gagacgccat tcttggcaaa acagcggagg atggaacatg gagttcctca tcgggcaatg
     1141 gcacgaaacc aacgattttc aggccgcggt gcacaggatc tttgccagct tgatcgacga
     1201 attccttggc tttgccgccg ctgcccagca cgatggcctg ggactcgaga atgccaaggc
     1261 tggaccattt gaaaaaacct gcccgggtca acaaaatgcc cggcaaagca acaagcagca
     1321 caagcccgag tattccacgt ccaagataga gatcgggcag gaggtaaaaa accagcgtca
     1381 tgattccaaa cccgagcatc atggagggca tcagacgaaa taaagtatcc ttgaggctct
     1441 tgcgtgaatc tttttgatac atgcccatcg ccgccattga cgtgatcatc gcgaacgtga
     1501 atgttccagc ttccggaaac agggaaggaa aagtgaaagg aagaggcgca gcctgatcaa
     1561 aaaaacgaat acctgccgca aagtaaacgg cgaaaatcaa cacgagcatt tccgcgctga
     1621 taagtagaac cgtggttctg gagatatagt gattcgagat acgtagcaat ttgcagacct
     1681 ttcaccgatc gttgctgaac aaccggcgag ccggttcttc ttctgtctgt ttacccgttt
     1741 actcggccgc ttgctttctg acaaccagta tcaggtatgc gagccaaata ggcatcagca
     1801 taagcggtct ttttactagc gctgtcgtct gccgcgcatc ataccaattc aggaatcgag
     1861 aacaattgga ctttggtaca tccgccagag cctcttttgt atcgcctcgt gaccgattga
     1921 acaacagctg aggctgacag taagcagcgt gttccagaat gatccgcggg ggacgcgcac
     1981 tcatgtcaga aatagcaagc gtgcctgcac gatcggacgc catggaacga tgcacacggg
     2041 taactgttac acaagctatc agtgcaggcg ctgcacgaaa tgaggataag ctcggcaatg
     2101 ttttaatagt tgtggttatt gtttgtttgt taatggttga ttgccatctg tattacggtg
     2161 ccggctgcaa acatcgattt tgtgccggat aaatgattaa tgttcagctt gcgttgtcgt
     2221 actcgatgac accgagcctt ttacgatgag ggccagcttg aggcggtccg caacgtccag
     2281 ttttcggaaa acttcagtca agtgagcctt tactgtacgc tctgtgatcg ccagacggcg
     2341 tgcaatttgt ttgttcgatt ccccgtttcc caccagcgtg gcaatttcat attcccgcct
     2401 tgtcaggttt gaaagaagtt ggctcactgt acgcttgatc cggttctgct cgcgcgtcat
     2461 ggtgacaagc tcgtttaaaa gccgccagct cagggacctt ctaatccata gctcgcctct
     2521 tcccaccgcc tcgacgacag agctgagctg ttcggcctcg atatcgctcc tgcaacagcc
     2581 ccttacccct attctaaata attcccactc tatctcttcc gtaaccgtct ggctcaagat
     2641 gatgaatttc gtttccagac tcaacttcat cagtattgct atcccctctg acccgtcgat
     2701 gcctggaaag tcgcggtcaa gcaataggat ttggggcttt ccccggacga gttgatcgtt
     2761 cagcaaggcg agattttttg aaacgaaaac tggatccaga tgatgaatac tgcgctccca
     2821 gcgagataaa ttatcctggg atgaactagc gagcaaaatc aagagagcct ctccccaaaa
     2881 gcgaaatatc ggtgcaaacc ttatccataa ttcgacccag catctttcgt gatacccaga
     2941 tctcgccctg atcgaccaca cgcacggcct tcaggaaata acatcggtct gcctcatgag
     3001 tcaggtaccc acgtgcacca ttcgccaaag cgcgaagtat ctgctcctcc tcgatcaatc
     3061 cctccgtaag caccacgatg aaggtatcgg ggcactctcg gttgagcgcc tcgatcattt
     3121 ttagactcgc gctgccgaac gaatcgagat taaagaagag aatgcgaggc ctcaggcggc
     3181 ttacttccag cacaatgtct tccgtgcaag cagcgttcag tgcggctgcc ttacgactgt
     3241 gcgccttacc gctcgcattc atgacgttcg ccatcacctc gatgcctgcc ccacctttta
     3301 acaactgttc cagcttcttg cgtcggccag gatccctatc ggctatcgct actgtaactg
     3361 tttgcatgct tcctccttac aggaccgccg gccactttcc cggttcaggc gaggtgactc
     3421 ccttgtcgtt tctcgggctt cacgattggc cgccaagttc aacttgaagt tgttcgaaga
     3481 catcctaacg tatgggaaag tactggtaac cacgaagtaa aaggaatctt ccgccttaga
     3541 ttacttttta aaagggtcac cagaactctc ttcattgccc tgcttcagca gatccctaat
     3601 gtagtttcca ggaatggcgt acgttatccc gctggggttg ctgagagcag actcttttgt
     3661 gcccttcaca aataccatgt tgatcacgcc gtaaaccagt cccgtttcgg gatcgaacag
     3721 cgggctgccg ctgctgcccg gataagccgt gccatcgagc tgaaatactg cgtaacgtgg
     3781 cttctgcaac tggccaatca tttttgaatt gagttgcctc gaattatgtg caggcaagat
     3841 gatcggagtg atcgcagaaa tcattgcacg atgggtgacc ggatagaatc ctaaaaccat
     3901 cccgatggga aagccggtaa aaaccagggt ctggccttcg cgcaccctgt gggcatcccc
     3961 cagctccatg gcaggcaatg gcgttcccgt aattttcagt agtgcgagat catgctccct
     4021 gtcaactgct gcgactgtcg cagttctcaa ttcaggttct ttacccttgc cagtaatcac
     4081 aacgtatgac tctttgttga ccgtatccaa tatttccgga acaacatgag cattggtcac
     4141 aatatgaaaa ccgtcgtcca gcacgaagcc cgtgccgaga aaattaagct gaggtgaccg
     4201 ggttctttgt agcgtcccga tccccacaat ggatggcttg atgcgctcga tggttttaac
     4261 aagctcgcta gcccacagcg aaggagcgaa cagaagcgct gccatggcca ggcttttagc
     4321 ataactccgg acgagacggg gaagtgcttg cctgatattc aacattgatt tccgctgggc
     4381 attgcaaatg gtattcggtg caagagaatc aactggagaa caggcagaat tctgcgcttg
     4441 ccgggccgca aaataaagca gcccgacaaa cactgatcaa gcgatcaggt tgtacggcaa
     4501 aggaccaggc catgcagaga acagcagtgg caatccctgg agggtcaggt agtgcgggcc
     4561 cagagagagc aggcacatgc cctgtgggga tcagcgttat gtaaagcggt cagcttcaca
     4621 attcaccgat tatcggcaac cttccatttt gtatatgaaa taaccgtctt tattgatggc
     4681 atcctcaaca ttggcaggag cgctttggct gtggcaaaaa ctacattccc tgctcgtcac
     4741 cgttgcatcg ccttccccga tcgatgccaa ccactgcttc gcgtattcga ttgctttctg
     4801 atggtccttg gcaggggtaa acacatcaaa atgcatcgtg tgaccatcct tggccttgac
     4861 ataggtgtca tatacatgaa tttccatatt gctcctcatt gcatgaataa gtcggttgaa
     4921 acattaactg atcaatatca tcagatcaac cggtagacta tcacggcagc tcaaaaagtg
     4981 aaacttacga gccgtcgacc cgggtgcttc tcccttccat ccctcatctc cgtcagcaaa
     5041 ccgggtgaac agcccagccg gacttcttcg cgaaacccac tgcttcctgt cataaaactg
     5101 tgacaaaact gcaataaaac ggccatatcc ctccactatt ctcccaatgg tttgttcatt
     5161 gaaaagtcga aagctgaacc gggagaagaa tgaaattatc gaaaatcagt ttggcggccg
     5221 gcgctatttt gggctctagc gtcgcacccc tggtgtttgc cattgatctt tatgtcgaca
     5281 cgaaaaccga gcaaatatat gcgaagcccg gaccgggccg cacccatatc ggttctttcg
     5341 tgagagaaga tgccgggctg aagaaggaga gggtatccac cggcgggacg tctgaaacag
     5401 ataaggcttc tgacaaaacg gccgaaataa tgaacgatag gacggatgcg gctgagttgg
     5461 cagccatgcg caaggacatg gaattaaaag cgaaaatgag agaagcgcgg ttggtcagtg
     5521 atgtcgcttc actggaggaa cgtgtcaagc aggctgaaaa ggtgcacgtc gagcttaaag
     5581 aaggggctcc ccatttcgcg agcaaggacg gcaacttcac ctttgcactg aacggacgca
     5641 tgcaggtggg atcgcaatat aatttcatca atgaagtact gcctgccacc ggaagcaatc
     5701 tgcccaacga attgaacagc ggagtaacgc tccggcgcgc ccggcttggc gttgaaggca
     5761 ccttcttcaa aatatgggat tacaagttcg agtatgactt cagccggggc aacggtacag
     5821 tagagtcagg aataaccgat gcctttgtcc gcctcaacgt aaacaagcca ttttccctca
     5881 aagtcggttc gttcaaagaa cctttcagtc ttgaggaagc cagcagcaac cgctttctga
     5941 ctttcatcga gcggcacatg gcggtcaact cgtttgttga caatcccaat gcctacaaaa
     6001 cgggtatagg cgcgaattat gcagagcccc gctttcagtg gggcgttgct ttacaaacag
     6061 aacctgtggg atcttggtct gccgcttcca ctacagtcaa cactgccggc aacaatagcc
     6121 gcaataacgg cgccggggat atgggctggg aaggcattgg ccggataaca ggaagaccgt
     6181 ggatggaaaa tgaaacgaag ttcctgcatg tcggagtttc ggcaggccac acggtcgtca
     6241 atacgcaata ccaggctaat ggcacgatca atgttggacc gaaccaaacg ggaggaggcg
     6301 gagggatggc gttctttgca ttcccgggaa cgaatgtgga ccgcactaat gtgctgaaca
     6361 ccggtaactt gagcatcgga aataaaggcg cacctggctc gcgccagata acgagctatg
     6421 accgctatgg ggcggaaggc tggctggttc acggcccgct gtctctccaa gccgagtatt
     6481 tacggacgaa tataaacgga gtgggttatg acaacgagca cttcaccggc tactatggat
     6541 tcgccagtta ttttttgacc ggcgagtcca aagcctatca catcagaaat gctgcagcga
     6601 accggataaa acccagacag aattttcaat ggaatggccc aggctgggga gcttgggaaa
     6661 tcgccgccgg ttacgattat atcaacctga acagcggtgt catcagaggg gggcaagcgg
     6721 acatggtaag gtttgctttg aattggtacc ctcataccca tataaaggta cagaataata
     6781 ttacttatgt tttaaatatc aatacggaag ggtcgaccat tccccgcact gccggcttta
     6841 ataacgcgga cttaagctct ttcctgactc aagttcaagt cgatttctaa gaaggccgtc
     6901 cttataaaac ccgaataaac cgcacgattt acaacgttgc ctattcatgc tcgtccgcga
     6961 tcaaaacctc cagggcaaag gctgaactta cctgagcgaa aactcaactt tgctgccggg
     7021 ctttttattg cccccttccg cgtccacttt gggttgaagc agaaagatcc ggtcgccgga
     7081 aatatcgccc ttttcaacca gccaatcacg tgcattttcg gccctggctt gggcgaggtc
     7141 ccgcatttcg ctctcgccgg catcaatgtt ggcgagcatc aattgttcca tctcgggcac
     7201 tggcaagctt ttcgtgaacc cgatcacgtt ttttggctta tcgaattttt catccttgta
     7261 tgcaagcgtg aggtatttgg cgtactcgtc cggcgtcaac gcgatatcgt caagtgaacc
     7321 gctcgtttct cccttcttga cgttttccag cagtttccgc gccttgatct tccgttccag
     7381 catgacacgc ttcaatgcct ccgggtcgta ggccggatcg gcctcaccgg tgatttcaag
     7441 ctcgagcgca ggcctgtctt ttaaggcctt tgacaaaaca tcgagactct tttctgcttc
     7501 gggaccaaga tccgcgcgac caggctcaaa gcttatctct gataattctt cgccgctgcg
     7561 tccaaaaacc gatccgagca gcgcgaaagg tgctgtcacg gccttcgaga gcagattaac
     7621 gaaagcgttt acgacgacgg agccaatgct gaactgcgga tcgttgatgg aaccggttac
     7681 cggaaggtga agattgattt ctccttcgcg gtttttcagc agcgcgactg ccagggttac
     7741 cgggatcgag agcgcgtcag ggctcttgac tttttcaccg aaggttagtt tgtcgagaaa
     7801 aacgttgttc tccgcggtaa gttcaccctt ttccacgtga tagtggacat cgacggacag
     7861 cttgcccttc tcaatggtat agccgatgta tttcgcggaa taagggctga acgccggcat
     7921 gtcgataccc ttggccgacg cagtgacgtc gaggtacgtt tccttcgcga aagtgttgac
     7981 cttgcccgat attttcaggg gagccgtttt gtcaaccgct ccatgaatgt cgatctcccc
     8041 cggcttttgg ggatcgaggg ggccgatacg gcccgcaaga cctgtcaggt tcgccttgta
     8101 attcggcttg atgaattgat cctggaaatt gatgttcccc ccttgcaatg atatccggct
     8161 gatacgtaca ggcgttgatt tccggtcggc tggagctgtc gttttggcac cggtcttcga
     8221 ggctgtcgta ggtcccggtt cggccgtttc tgcgggtggc ggcgggggaa ccggtgattc
     8281 attattcccc tcctgccgca caatatgcgt gagattgagg tcgcccttgg aagtgattac
     8341 aacgtatgca aaaaaatcag caattgaaac cgcttctata ttcgcccgca ggggttcgtt
     8401 cacgaactcg atgcccctga tgtccagggt gcgccaacgc atgaggtcag tggtcgccga
     8461 tttatcgagc acgctaaaat tggtgaattt cccatccccg agcaatacga ctttaatcgg
     8521 cgcgccatcg gccttgatct tgccatgaaa tgacgtcgcg ccactcgtga tcaaggcgtt
     8581 catcttgtct cccgcccagc cttgtagcgg cacaagatcg acatccttcg cgtccacatc
     8641 gagctcgacc gcgagtgggg cccatgcgag cgtgccattc gtctccaggc ttcccttttt
     8701 atttaccgct gcttttagcg caattttcag cggctccgcg ccacgaagat caatatctgt
     8761 gacagtcagg tcgagtggtt gaacgatcat cggactcacg ttcgtcagcg aaacatcttc
     8821 ataacgaagg gcagcgttgg tcaacttgaa gctgccgaac cgtacctccc acggcttatc
     8881 ggcttttgct tcgggagcga tcgctttccg gggggccgtc gagcgggaag cgggcgtaaa
     8941 taactttgcg agattgaggc ggccgtcact ttcccgccgc aagaatgcat ggattcggtc
     9001 gagcgttata tccccgagcg tgacagattg atgcgcgaga tcgatggagg attggctcac
     9061 cttcaatgat ggaaggctca gcacaggttg ttcttctccc tcgcgagaga tcgcggcttc
     9121 actcaacgtc agggcaacgt tcgatcgctt tgcactgttc agattgagct cgactggctt
     9181 gatctggatt tccgcgatct tggccgcgta tggtatctcg gtaccgtggt tctcgattcc
     9241 cagttttcga agtgctgctt ctc
//
LOCUS       DASSHJ010000135         8230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_47359, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000135 DASSHJ010000000
VERSION     DASSHJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47359"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFI43_05305"
     CDS             complement(<1..253)
                     /locus_tag="VFI43_05305"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF017768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HET7061576.1"
                     /translation="MSGIIRSDPWQNFRRFTPARIALGSAGNSLPTEEVLRLGLAHAE
                     ARDAVHLPLDTAALEEGLRAMSCHTLRVQSRAADRATYLL"
     gene            complement(250..1647)
                     /locus_tag="VFI43_05310"
     CDS             complement(250..1647)
                     /locus_tag="VFI43_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HET7061577.1"
                     /translation="MAYSYSVRNITYRFNDLKDLLAKASPPRSGDYLAGVAAATAVER
                     MAAKMCLADLPLPVFLSEAVIPYEADEVTRLIMDRHDPVAFAEISHLTVGDFRNWLLA
                     DSTDEATLVRIAAGIMPEMAAAVSKLMHNQDLILAARKCRVVTRFRNTIGLPGRLSVR
                     LQPNHPTDDLKGIAASMLDGLLYGCGDAVVGVNPAMDNLSGLIKLLDMLDEVRRRFDI
                     PTQSCVLTHVTNTILAIEQGAPVDLVFQSIAGTEKANAVFGIGLALLGEAREAALSLG
                     RGTLGDNVMYFETGQGSALSAEGNFGVDQQTCEARAYGVAREFKPLLTNTVVGFIGPE
                     YLYDGKQIIRAGLEDHFCGKLLGVPLGCDICYTNHAEADQDDTDTLLTLLGTAGVTFI
                     MGVPGADDVMLNYQSTSFHDALYLRSLLGLKRAPEFEIWLEKMKITGARGELLRTPPD
                     HPLLSVLPEAAGAGS"
     gene            1874..2422
                     /locus_tag="VFI43_05315"
     CDS             1874..2422
                     /locus_tag="VFI43_05315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7061578.1"
                     /translation="MAAAFADDPLTTFCVRADAQRYPAMQAGFKRALELYRPHGLTFI
                     ANDGTGVAIWARHDQWQLTFAQECALIPLYVYVCGVGRFMRLVRGIEALKSHHPVEPH
                     YYLYMLGVHPDYQSQGLGSALLRIMLERCDREHMPAYLEASHPRNISLYQRHGFRLMR
                     ELQFGPGGPSLRAMWREPRIGS"
     gene            2671..3078
                     /locus_tag="VFI43_05320"
     CDS             2671..3078
                     /locus_tag="VFI43_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061579.1"
                     /translation="MKNAKNISTSKNWSQSLWVTALMFAVAGAVHAQGGSSGEGGGSS
                     SAKAAKTTAKSGSGAQGKSQGGSDGWDQYTYGKDSARDYARSQGVGHSGGTSYAYGKD
                     KGSDYERSQGMGKGTSGGRSPGGGSSSSQSDYD"
     gene            complement(3153..4772)
                     /locus_tag="VFI43_05325"
     CDS             complement(3153..4772)
                     /locus_tag="VFI43_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061580.1"
                     /translation="MTSLDKTWMAFAAICTVCSTSISAAQPISLAPAAMPAIGTVDVR
                     YQSYNVEMAEVVGGNFWKPYAKKSLATPSAGPTSGPSKSGLSTRGDGPQQIGESSILE
                     PRPPINLADPRLRKLAAALGPAFVRVSGTWANSVYFHDSDTPAPTTPPKGFTSVLTRN
                     EWQGVVDFARKVDAKLVTSFAFSPGVRNEAGVWTTDQAESFLDYTKSLGGEIVAVEFV
                     NEPNLPTEGGAPPGYSASAYARDFAIFHRFIKAKVPGMRIAGPGTVGEGDALSITATS
                     RPLFTTLQILAALAPPRFDIFSYHSYPAASVRCAFFGHTAQTVEEDALSDEWLARSDP
                     IHAFYLRLRDRYEPRRPAWITEIADAACGGNPWASTFLDSFRYLDQLGRLAKTGIQVV
                     FHNTLASSDYGLLDQDTFKPRPNYWAALLWHKLMSAKVLDAGRPWPGLKLYAHCLAGH
                     PGGVSILAINNSRTDQRSLELSLASQRYRLTSQPLDSPRIELNGNELKLQANDELPAL
                     RGDHVPPGYIELPPASITFLAVRDARNADCK"
     gene            4865..5359
                     /locus_tag="VFI43_05330"
     CDS             4865..5359
                     /locus_tag="VFI43_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7061581.1"
                     /translation="MQMKQIKQKVSPALWFDNNGEEAVNFYVSVFKNSRINQIIRYGE
                     AGPGPVGTVMAITFDLDGEEFTAINGGPHFTFSPAISFVVKCINQDEVDYYWEKLSAG
                     GRQDQCGWLADKFGVSWQIVPVTLLELLDDPDPAKSKRVMEAVLQMHKIDIELIRQAY
                     AQTP"
     gene            5368..6303
                     /locus_tag="VFI43_05335"
     CDS             5368..6303
                     /locus_tag="VFI43_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET7061582.1"
                     /translation="MLGVGIALGNGPSGNTYTIKQMIRSDTIEITPEIHALIARVDEF
                     RRAWRAMGELAPEKRLPLRESAMVEAIGASMRIEGSPITDAQAEHVLQALSTDLSGSF
                     STSWEQEAAGYARLIETIFSSWRNIPFSESSIKEMHGELLGQSEKDAWHRGHYKAISS
                     GSEDSVSDEVKAGLGARDVIPLETPRLMSELMGWLKEEQSAKRLHSLLIIALWVVVFL
                     KIHPFQEGNAPLSRMLTTLLLLQSGYSYVQYSSLESIIEQNKEAYDLALRQTQETIGT
                     DSPNWQPWLVFFLRSLAQQVTRLEQKIERKTEPRD"
     gene            complement(6300..6803)
                     /locus_tag="VFI43_05340"
     CDS             complement(6300..6803)
                     /locus_tag="VFI43_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HET7061583.1"
                     /translation="MIEKIISGGQTGVDRAALDWAIANDIGHGGWCPAGHRAEDGIIP
                     EHYSLQETPGRGYSERTRWNVRDSDATLIISSSPELSGGSLATKQYANKMDRPCLHIY
                     PGDEWDASLRGFIKKHPIHILNVAGPRGSAWANPGPFVHEVLDKISPNCKHLGKRTVC
                     MDNGQCP"
     gene            complement(6841..7161)
                     /locus_tag="VFI43_05345"
     CDS             complement(6841..7161)
                     /locus_tag="VFI43_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061584.1"
                     /translation="MEYNEELVQQVWEKARVNSIVPMTQWREDECGAWIARQHYGDTD
                     SEFGWVILNVSAGRPDSIENLRAFHHRNSYDIANQNAKCHITADRTDLPSFEHSPQPR
                     NRDV"
     gene            7416..>8230
                     /locus_tag="VFI43_05350"
     CDS             7416..>8230
                     /locus_tag="VFI43_05350"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7061585.1"
                     /translation="MSAPSENRTISVSALELERHVRTLAEEIGPRHIVRPHALAAARD
                     YICNQWQAGGYVVEMQEYKARNIPCMNVIITLPGISSPEQILLAGAHYDTVPRAPGAD
                     DNASGVAALLELGRCLAGYQPGRTIRLVAFVNEEPPFFYWSQMGSAVYARAARQRGED
                     IRAMFSLEMLGCYSEEPRSQRYPPFLGRGRPDCGNFIAFVANLHSRPLLKQALAAFRT
                     GSDFPVESTAAFGWLPGINWSDHLNFWRSGYPALMITDTAFFRYRYYHSPQDTP"
BASE COUNT         1815 a         2258 c         2295 g         1862 t
ORIGIN      
        1 gcagcagata ggttgcacgg tccgcggcgc ggctttgaac tcgcaatgta tgacaagaca
       61 tcgctcgcaa accttcttcc agcgccgccg tatctagagg tagatgtact gcgtcgcgcg
      121 cttcggcatg tgccaggccc aggcgtaata cctcctcggt cggcaaactg ttgccagcac
      181 tcccaagagc gattcgtgcc ggggtgaagc ggcgaaagtt ctgccaggga tcactccgga
      241 taattccgct catgatcctg cacccgctgc ctcaggaagc acgctaagta atggatgatc
      301 aggtggggtc cggagcaatt cccctcgcgc cccggtaatc ttcatttttt ccagccatat
      361 ctcgaattcc ggcgcgcgtt tcagtcccag gagggaacgg agataaagcg catcatgaaa
      421 cgaggtgctt tggtaattca acatcacatc atccgcaccg ggtacaccca ttatgaacgt
      481 cactcctgcc gttccgagca gggtgagcaa cgtatccgtg tcatcctggt ccgcttccgc
      541 atgattggtg tagcatatat cgcaccccag cgggactccc aggagctttc cacaaaaatg
      601 atcttccaat cccgctcgta taatctgctt gccgtcgtat aaatactcgg ggccgatgaa
      661 gcctacaact gtgttggtga gaagcggttt aaactcccgc gccaccccgt acgcgcgggc
      721 ttcgcatgtc tgctgatcta cgccgaaatt cccctccgcc gaaagcgcgc tcccctgtcc
      781 tgtctcgaaa tacatgacgt tgtccccaag ggtacctcgc cctagcgaca acgcagcctc
      841 gcgcgcctcg cccaataatg ccagaccgat gccaaagaca gcattggctt tctccgtccc
      901 ggcaatagac tgaaacacca gatctaccgg cgctccctgc tcgatagcca gaatagtatt
      961 ggtgacatga gtgagcacgc aggactgggt ggggatatcg aagcggcggc gcacttcatc
     1021 caacatatcg agaagtttga taagtcccga aagattatcc attgccggat tgacacccac
     1081 gaccgcgtcg ccgcacccgt aaagcaatcc atcaagcatg gaagcagcaa tacctttcaa
     1141 gtcatcggtc ggatgattgg gttgcaagcg cacagataat cgtccgggaa ggccgatagt
     1201 attgcggaag cgggtgacaa cacggcactt tctggcagct agaatcaagt cctggttgtg
     1261 catcagttta ctgaccgcag ccgccatctc cggcatgatg cctgcagcaa tacggacaag
     1321 cgttgcttca tccgtgctgt cggcaagaag ccaattgcgg aaatcaccaa cggtaaggtg
     1381 actgatctcg gcgaaggcga cgggatcatg gcgatccatg atgaggcgag tcacttcgtc
     1441 ggcttcgtaa ggtatcactg cctcggagag aaaaacaggc agcggaaggt cggcaagaca
     1501 catttttgca gccatacgtt ctactgccgt cgctgctgca acgcctgcca gatagtcgcc
     1561 tgaacgtggc ggagaagcct tggcgagcaa atccttgaga tcgttgaagc gatatgtgat
     1621 atttcggacc gagtaggaat aggccatttt cgatgttctg ccattacaaa gcttcaagct
     1681 caaccatgct caccggggaa aatcttccta acgaattaca gatcttcttg ttgttccagt
     1741 tcatgcgttt atttccattg tagcaacacg gataaaggat tttgcaaacc tgagggatct
     1801 ccagacaagc ctgcaatctc agcccctgat ccggcgagct gaacagtctg atgattcgac
     1861 gctcgcgctg accttggctg cggcgtttgc cgatgatccc ctgaccacat tctgtgttcg
     1921 cgcagatgcg caacggtatc cggcgatgca agcgggtttc aagcgcgcgc tcgagttata
     1981 tcgcccgcac ggcttgacct tcatcgccaa cgacgggaca ggggtcgcaa tctgggcacg
     2041 tcatgaccaa tggcaactta catttgcaca agagtgcgcc ctcattccgc tgtacgttta
     2101 cgtctgcggc gtgggtcgct tcatgcggct tgtccgtggg atcgaggcgt tgaaatcaca
     2161 tcacccggtc gagccgcact attatcttta catgcttggg gtgcatcccg attaccagtc
     2221 gcagggcctg gggagtgcgc tcctgcgcat tatgctggag cgttgcgacc gcgaacacat
     2281 gcctgcgtac ctggaagcat ctcatcctcg caatatttcc ctctaccaga ggcatgggtt
     2341 tcgcttaatg agggaattgc agttcggtcc gggcggccct tctctcaggg caatgtggcg
     2401 ggagccaagg atcgggagct gaatggagcc tgtaggcgat tgaggcaaac gtctgcttaa
     2461 cggctcatcc gaacctggct gttcctcctg gccttattct gacagtagcc ctgcggtacg
     2521 tcctcaagga tgcttaccca tcggcgatgc gagcggcagg acgcgttgta ctcctgcggg
     2581 ctaaggtcta gttcgtgcgc caccgtacgg aatatttact gtgcaaccgt atcatcttgt
     2641 ttggaccaat ccctaacaaa cgaggacgcc atgaaaaacg ctaaaaatat ctccacatcg
     2701 aaaaactgga gtcaatcgct atgggttact gctcttatgt tcgccgtggc cggggcggtt
     2761 catgctcaag gcggcagctc aggtgagggc gggggttcgt catctgccaa ggcggccaaa
     2821 acgactgcaa aatctggatc aggggcacag ggaaaaagcc aaggcggttc cgacggctgg
     2881 gaccaatata cctatggcaa ggactccgcg cgtgattacg cgcgttcgca aggggtcggc
     2941 cattcggggg gaacgtctta cgcatatgga aaagacaaag ggagcgacta cgagaggtcc
     3001 cagggaatgg ggaaaggaac gtcgggcggg cgctctccgg gcggaggctc cagctcttcg
     3061 caaagcgatt acgattgatc actggataat accctgcaaa aaaacacgct ttttttattc
     3121 ttcaagcata acacggcctt ctggtaatcg actcatttgc agtccgcgtt cctggcatct
     3181 cgtaccgcca ggaaggtgat gcttgccggg gggagttcga tatagcctgg agggacgtgg
     3241 tcgccccgca acgccggaag ctcatcgtta gcctgaagtt tgagctcatt tccattcagt
     3301 tcgatccgtg ggctgtccaa cggctggctt gtcaacctgt atcgctgtga cgcaagcgat
     3361 agctccagtg atcgctggtc agtcctgctg ttattgatcg ccaggatgga aacgccgccg
     3421 ggatggcccg ccaggcaatg cgcatagagc ttcaatcccg gccaaggacg ccctgcatcg
     3481 agcaccttcg cactcatgag cttgtgccat aacagtgccg cccagtaatt cgggcgcggc
     3541 ttgaaggtgt cctgatcgag cagaccatag tcgctggatg caagggtatt gtgaaagaca
     3601 acctggatac ccgttttggc gagccgcccc agctggtcga gatagcggaa gctgtccaga
     3661 aaagtggagg cccaggggtt gccgccgcat gccgcatccg cgatctccgt gatccaggcc
     3721 ggtctgcgag gttcataacg atctcgcaga cgcagataaa aagcatgaat cgggtcggaa
     3781 cgtgcgagcc attcgtctga taatgcatct tcctctaccg tctgagcggt gtggccaaag
     3841 aatgcgcatc tgaccgaggc ggcagggtag gagtgatacg agaagatgtc gaaccgtggc
     3901 ggagcgagcg cagcgaggat ttgaagggtc gtgaacaaag gcctgctcgt ggcggtaatc
     3961 gagagcgcat cgccctcgcc caccgttcca ggacccgcaa tccgcatgcc aggaaccttg
     4021 gctttgataa acctgtggaa tatcgcgaaa tcacgtgcgt aggctgaagc gctgtatcca
     4081 ggcggcgcgc ccccctcggt tggcaggttt ggttcattaa cgaactccac tgcgacaatt
     4141 tcgccaccca gggatttcgt ataatcaaga aaactctcgg cctgatcggt tgtccagacg
     4201 ccggcctcgt tgcgcacacc ggggctgaaa gcaaacgacg taaccagttt tgcgtccact
     4261 tttcgggcga aatcgacgac accctgccat tcgtttcgtg tcagcacgct cgtgaaccct
     4321 ttcggtggag tcgttggcgc aggagtatcg gaatcatgga aataaacaga atttgcccat
     4381 gtcccgctta cgcgtacgaa ggccgggccg agggctgctg cgagctttcg caggcgtgga
     4441 tctgccagat tgatcggtgg ccgcggctca agtatcgaac tttcgccaat ttgttgcggc
     4501 ccatcgcccc gtgtggatag acctgacttc gatggcccac tcgtagggcc cgcgcttggc
     4561 gttgccaggc tttttttggc gtacggcttc cagaaattgc ccccgacaac ctcggccatc
     4621 tcgacgttgt aggattggta acgaacgtca accgtgccta tggcaggcat ggcggcaggg
     4681 gcgagcgaga tgggctgggc agccgatatg cttgtactgc acactgtgca gatggctgca
     4741 aacgccatcc atgttttatc cagggacgtc atgtcgcatc aatgattgaa tggtttcgca
     4801 tcgtaatgat caataataaa gttagcaccg attaccctta tttatttcga aaggaatctc
     4861 atccatgcaa atgaagcaaa tcaagcaaaa agtctcgcct gccctgtggt tcgataacaa
     4921 tggggaagag gccgtcaatt tctatgtatc ggtcttcaaa aactccagga tcaatcagat
     4981 catccgttac ggcgaggccg gtcctggccc ggtgggaacc gtgatggcaa ttacatttga
     5041 tctcgacggg gaggaattca cggcgatcaa cggcgggccg cattttacgt tctcgcccgc
     5101 catatccttc gtcgtgaaat gcataaatca ggatgaagtg gattactact gggaaaagct
     5161 ttccgccgga ggacggcagg atcagtgcgg ttggcttgcc gacaagttcg gtgtctcctg
     5221 gcaaatcgtg ccggtgactc tgctcgagct gctggacgat cctgaccccg ccaagtcgaa
     5281 gcgggtaatg gaagccgtgc ttcagatgca caagatcgac attgaactga ttcggcaagc
     5341 atacgcgcag acgccatagc ccaagtgatg cttggggtcg gcattgcgtt gggtaatggc
     5401 ccgagcggta atacgtatac tattaaacaa atgatccggt cagatacgat agaaattaca
     5461 cccgagattc atgcgttgat cgcccgtgtg gatgaattca gaagggcgtg gcgggcgatg
     5521 ggcgagcttg cgcccgaaaa acgcttgcct ctgcgcgaga gtgcgatggt cgaagccatt
     5581 ggcgcttcaa tgcgtatcga aggcagtccg attaccgatg cgcaagccga gcatgtactt
     5641 caggccctct caacagacct cagcggatcg ttctctacat cctgggagca agaagctgcg
     5701 ggttatgccc gattgatcga gacgatattc tcttcctggc gcaacatccc cttttcagag
     5761 agcagcatca aggaaatgca tggagaactg cttgggcaaa gcgaaaaaga tgcctggcat
     5821 cgcggccact ataaggcaat ttcttctggc agcgaagatt ccgtcagcga tgaggtaaag
     5881 gcaggtttgg gcgcccggga cgttattcct ctggaaacgc cacggctcat gtccgaactg
     5941 atgggttggt taaaggagga acagagcgcg aagcgtctgc actcgctatt gattatcgcc
     6001 ctgtgggtgg tagtgtttct taaaattcat ccattccagg agggtaacgc gccactgtcc
     6061 cgaatgctga caactttgct gctccttcag tcgggttatt catatgttca gtacagttct
     6121 ttggagagca ttatcgagca gaacaaagag gcctacgatc tcgcgctcag gcaaacacag
     6181 gaaaccatcg gaacggattc gccgaactgg cagccgtggc tggtgttctt cctgcggtct
     6241 ttggcacagc aggtgacccg gctggaacag aaaatagagc gcaagaccga gccaagggat
     6301 taaggacatt gcccgttatc catgcaaacg gtgcgtttgc caaggtgctt gcaatttgga
     6361 cttattttat ccagaacctc gtggacgaaa ggtccaggat tagcccaggc tgagcctcgc
     6421 ggccctgcaa cattgaggat gtggatgggg tgctttttga taaagccgcg cagtgaggcg
     6481 tcccattcat caccgggata aatatgcagg catggccggt ccattttgtt cgcatactgc
     6541 ttcgttgcga gcgaaccccc gctcagctcc ggcgatgatg aaataatcag agtggcgtcg
     6601 ctgtcgcgaa cgttccacct tgtgcgttcg gaatagccgc gtcccggcgt ctcctgcaac
     6661 gagtaatgct cgggaataat tccatcttct gcccggtgcc ctgccggaca ccagccacca
     6721 tgccctatgt catttgctat tgcccaatcg agggcagctc gatcgacacc cgtttgtccg
     6781 ccggagatta tcttctcaat catcgggctt cgggtgcggt atgcttttca aacgccccgt
     6841 ttaaacatcc ctgttgcggg gttgcggact atgctcgaag gaaggcagat cggtgcgatc
     6901 agccgttata tggcatttgg cgttctggtt ggctatatcg tagctgttgc ggtggtgaaa
     6961 cgccctcagg ttctcgatgg agtccggtct gccagccgag acattgagga tcacccagcc
     7021 aaactcggaa tccgtatctc cgtaatgctg gcgcgcgatc catgcgccgc attcatcttc
     7081 ccgccattga gtcatgggaa caatggagtt gacgcgtgcc ttttcccaca cttgctgaac
     7141 cagttcttcg ttgtattcca ttcctcctcc tcgaatttgt cgccggcgtc tgccatttgc
     7201 cggcctttgc gatgcttgcg agatacatat ccctgctgga gcgaagactt tcggcgccat
     7261 cgacacccac actttttact atattagttg ttcaggagaa ctgctcgttt tctgttttct
     7321 tgggcaactt tcttgcgcaa tttttgcaga atcttgtgaa agcacagcta ccgccgctgc
     7381 taacgttgct gaaataagtg ctaaaaggaa agtgtgtgtc cgcaccctca gaaaatagga
     7441 ccatctccgt ctcggctctc gaactggaaa ggcacgtgcg cacgttggcg gaggagatcg
     7501 gaccgcgcca tattgtgcga ccgcatgccc ttgcggccgc acgggactat atctgcaatc
     7561 agtggcaggc tggagggtat gtcgtggaga tgcaggaata taaggcgcgc aatatacctt
     7621 gcatgaacgt gatcatcacc ttgcccggca tctcttctcc cgaacagatt ctgctggctg
     7681 gcgctcacta tgatacggtc cctcgcgctc ccggcgccga tgacaatgcc agcggcgtcg
     7741 ctgccctgct ggaactcggc cgctgcctcg caggttatca gccgggccgg acgattcggc
     7801 tggtggcttt cgtgaatgag gagcctcctt ttttctactg gtcccagatg ggcagtgcag
     7861 tgtacgcacg agcggccagg cagcgcggag aggatattcg tgcaatgttc tcgctcgaaa
     7921 tgctgggctg ttacagcgag gaaccgcgca gccagagata tccgcccttt ttgggccgtg
     7981 gaaggccgga ctgtggaaat tttattgcct tcgtggctaa cctgcattca cgccctttgc
     8041 tcaagcaggc actggctgct tttcgcacgg gtagcgattt ccctgtagaa agcacggcag
     8101 ccttcggctg gcttccaggc ataaactgga gcgatcacct gaacttctgg cgctccggct
     8161 acccggcact catgatcacc gataccgcct tcttccgtta ccgttactac cactcacctc
     8221 aggacacgcc
//
LOCUS       DASSHJ010000136         9889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1085809,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000136 DASSHJ010000000
VERSION     DASSHJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085809"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1551
                     /locus_tag="VFI43_05355"
     CDS             496..1551
                     /locus_tag="VFI43_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7061586.1"
                     /translation="MKPGKSGTSTSGTSTADTSTSRRQFVGEMTAGLAVVLAPPALAQ
                     QAAVAVDSRSASGNLAKQDPTTQYPRPPFPPQRQEWPGLAGKMNPRPDHGETSYRGSG
                     KLTGRKALVTGGDSGIGRAAAIAFAREGADVAINYLPIEEPDAREVIDLIRAEGRKAV
                     ALPGDIRDESFCGKLVSEAVRDLKGLDILVNNAARQTAQPSIMDISTDQFDSTFKTNV
                     YAMFWITKAAMAHLQAGATIINTASVQAYDPSANLLDYAQTKASIVAFTKSLAKQVAK
                     QGIRVNAVAPGPFWTPLQPSGGQPPEEIPGFGGGTPLGRPGQPAELGPLYVLLASQDS
                     SYATGQVLGESGGSGQP"
     gene            1560..1844
                     /locus_tag="VFI43_05360"
     CDS             1560..1844
                     /locus_tag="VFI43_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061587.1"
                     /translation="MTPEREQPEQREQEETRRLSIRVECCGGYQGVQEPVAFWLGNRR
                     LKVQALVDRWFAPGQYWFKVDADDGNIYVLKYDENVGNWEIAAYRSGALR"
     gene            1987..3153
                     /locus_tag="VFI43_05365"
     CDS             1987..3153
                     /locus_tag="VFI43_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7061588.1"
                     /translation="MHAIPYWRLSGFYFFHFALMGAFVPYWSLYLQSISFTAFQIGVL
                     TSLLQGMRVFAPAAWGWLSDHSGKRVAIVQAAAAGGLLSYCAVFFGTSFGWLFVSMAL
                     MSFFWSASLPLMEATTLSYLREGIARYGRIRVWGSVGFICSVAGMGYMLDALGTASLL
                     WAVLGFNVGIVIFTSKIPEAGRSVHPAAGLSVSEIFKRPEVAAFFVACMLMALAHGPY
                     YTFYSIYLVEHGYSKSAVGWLWALGVISEVGVFFLMPQLVQRFRLKQIMVFSFTCGVA
                     RFLMIGWGVDSPSIVLAAQVLHAATYGSHHATAMLLVHHFFPGRHQAKGQALYISLTF
                     GLGGALGGLLSGYTWETLGAGLTFTVSAAAALTGLLLIGWKMQVDHPSDEYGDR"
     gene            3222..4655
                     /gene="leuC"
                     /locus_tag="VFI43_05370"
     CDS             3222..4655
                     /gene="leuC"
                     /locus_tag="VFI43_05370"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET7061589.1"
                     /translation="MQTLYDKLWQSHIVYEEPGSGSMDEQPKHDRMVLLYIDRHLVHE
                     VTSPQAFEGLKLAGRKPWRRESVLAVADHNVPTTGRSQGISDPVSRIQVETLDQNCEE
                     LNITEFRMNDVRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAFGIGTSEVE
                     HVLATQCLSTKKAKSMQVLVEGDLGHGVTAKDVALAIIGEIGTAGGNGYAIEFAGGAI
                     RALSMEGRMTLCNMAIEAGARAGMVAVDDTTIEYIKGRPFAPKGDLWEKAVAYWRTLK
                     SDEGARFDKIVRLDATQVKPQVTWGTSPEMVVAIDGKVPDPAQIENVVKRHDVERALK
                     YMGLAPNTPISEILLDKIFIGSCTNSRIEDLRAAANIVKGRRIASNIKLAMVVPGSGL
                     VKQQAEREGLDAIFRNAGFEWREPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGQG
                     GRTHLVSPAMAAAAAVAGRFVDASKIY"
     gene            4709..4834
                     /locus_tag="VFI43_05375"
     CDS             4709..4834
                     /locus_tag="VFI43_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HET7061590.1"
                     /translation="MKTFSALIALIAVFQLSACNTIGGMGRDMQKGGEAVERSSK"
     gene            4838..5476
                     /gene="leuD"
                     /locus_tag="VFI43_05380"
     CDS             4838..5476
                     /gene="leuD"
                     /locus_tag="VFI43_05380"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET7061591.1"
                     /translation="MEKFHPCEGLVAPLDRANIDTDAIIPKQFLKSIKRSGFGRNLFD
                     EWRYLDHGEPGMDSSQRKPNPDFVLNLPRYRGAQILLARANFGCGSSREHAPWAIQDY
                     GFKAIIAPSFADIFFNNCFKIGLLPIVLDVDSVDQLFHEVEAAEGYRLKVDLPDQAVT
                     TPGGQRFFFDIDPFRKHSLLNGLDEIGLTLQHADEIRAFEEKHRAAQPWLFA"
     gene            5505..6572
                     /gene="leuB"
                     /locus_tag="VFI43_05385"
     CDS             5505..6572
                     /gene="leuB"
                     /locus_tag="VFI43_05385"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7061592.1"
                     /translation="MKIAILAGDGIGPEIVAQAVRVLEALRKDGLKIELEPALLGGCA
                     VDAEGEPFPESTRKLVAEADAVLLGAVGGPKYDSLPRALRPEKGLLGIRKFLNLFANL
                     RPAILYPELANASTLKAEVVAGLDVLIVRELTGDIYFGEPRGIEHRGGQRVGFNTMVY
                     SETEIGRIARVAFQAARKRSRRLCSVDKMNVLECTQLWRDVVTETAEEYPDVELSHML
                     VDNAAMQLIRDPRQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLDQHNKGLYEPI
                     HGSAPDIAGKNVANPLATILSAAMMLRYTFDLEPAAARIEGAVKRVLAQGYRTADIYE
                     EGMKMVGTAEMGDAVLANISD"
     gene            6585..7697
                     /gene="asd"
                     /locus_tag="VFI43_05390"
     CDS             6585..7697
                     /gene="asd"
                     /locus_tag="VFI43_05390"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7061593.1"
                     /translation="MKRVGFIGWRGMVGSVLMQRMKEESDFALVEPAFFTTSQKGGKG
                     PDIGVEAPPLKDAHDIEELKAMDILISCQGGDYTSAIFGKLRESGWQGYWIDAASTLR
                     MGDDAVIILDPVNMPVIKQALHDDVKNFIGGNCTVSLMLMAVGGLFEADMVEWMSAMT
                     YQAASGAGAQNMRELLLQMGEAHRVAKDLLDDPASGILDIDRQVAGTLRDGNFPTDNF
                     GVPLAGSLIPWIDRDLGNGQTREEWKGQSETNKILGRSDRMVPVDGICVRVGAMRCHS
                     QALTIKLKQDVPLDEIKDILSASNNWVRVIPNERESTLKELTPAAVTGNLAIPVGRLR
                     KLAMGEEYLSAFTVGDQLLWGAAEPLRRMLRILVEA"
     gene            7829..9865
                     /locus_tag="VFI43_05395"
     CDS             7829..9865
                     /locus_tag="VFI43_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HET7061594.1"
                     /translation="MLKRRSVYLVAFALASPWMIDAAGAAGLGRLTVASIQGQPFKAE
                     IDLVAVKKEEKSLLTARLASQEGFRQASVDYLPVLSTFQATIETRANGAPYVRIVSPQ
                     SVSEPLLNILVELNWPSGRLLREYTVVLPHSEHDGNPIVKQARGPSPVVSTKVESAAN
                     KNRAGLRHDTNRPRVAEPVATVANSASYGPVREGDTLVGIVKNMTGARGASMNQTLIA
                     LHRANPDAFIGDNIHELKAGATLRIPDGNEISAIAPVEADRIVQLQTAEWRQRRLPLI
                     ARAPEELKQTVTGTIERTAELNLVSAKEPATEVLRLSKGVEPRGQEQVSANHAATPGE
                     KQHANAQDQSQAMEEDATARERALREANERVALLEKNIKELQRLLELKNVPLAEMQRK
                     AERMQPDPETLSPDALHPQAAPESVADGGAVAVARQSSENAEATGTKRTDSTRTTTSE
                     ATISDRIELAKPARSAKLNHEIDLSNTETGRRGKVSFLDGVMANFEYLGGALVLLLTG
                     IVGVSMVRGSKSSSVFESDYDDGTDSSTLPQKKGESGIVMRSEADPAGPSDVIHPQAS
                     PQSHRTGMSLTIPELCLEPAATPTPMKCESEGGASVSLVVAEARVEATTKPLPVEYET
                     ERDTNWPEILTKLDLARAYQEMDDKESARQILEEILREGDKRQREHVIAILNRL"
BASE COUNT         2179 a         2600 c         2978 g         2132 t
ORIGIN      
        1 tgatgagttc gacaggcaat tgctatcacg tccttcaagt tccttctgca ccgttgagcc
       61 aaagagcaca tcctcacgcg cggtcacccc gggctgataa tttgccgccc aacctccgcc
      121 gttgccatcc tgccatcgat ctcagcctcc tggattgtgc agcgatcaat gggcgaggcg
      181 gatatcgctc tcattctcca gatatcctgg caggttatcc ccaagatatc tcatgaacgt
      241 caaccgcacc ccttgttcgc cacttgctac cgagccattc atccttgaca gcccggaatg
      301 ggtgacatag gttgattaga gcaactgaat cttataacac ggcaataccg gcaggtccct
      361 ctaggaatgc tttgcttgcc ctttatcgac ccggccgatc ctcgttgagt tttacttcgc
      421 cggtttcgaa ccgatccctg gcgggacgat gtttctgatt gcattattcc ccaaaaattt
      481 tcacaggaga atgtcatgaa acctggaaaa agtggcacta gcaccagcgg cacaagcacc
      541 gctgacacaa gtacatcaag gcgccagttc gtgggtgaaa tgaccgcggg tctcgcggta
      601 gtgcttgcac cgcccgccct tgcgcaacaa gctgctgtcg cggtcgattc gcgcagcgca
      661 tccgggaact tggcgaagca ggatccaacc acccagtatc ctcgtcctcc ctttcccccg
      721 cagcgccagg agtggcccgg tctcgcgggg aaaatgaatc ctcggcctga tcacggcgaa
      781 acgagctatc ggggctcggg caaactgaca ggccgaaaag cactggtcac cggaggtgat
      841 tcaggtatag gccgcgccgc cgcgattgcc ttcgcccggg aaggagcgga tgttgcaatc
      901 aattatctcc ctatcgagga gccggacgcc agagaggtta ttgatctgat ccgggcggag
      961 ggacgcaagg ccgttgccct tcccggggac attcgtgatg agagcttctg cggcaagctg
     1021 gtctccgagg ctgttcggga cctgaagggg ctcgatattc tcgtcaataa cgccgcgagg
     1081 cagaccgccc agccttcaat catggatatt tcgacggatc agttcgactc cacgttcaag
     1141 accaacgtct acgcgatgtt ctggatcacg aaagctgcca tggctcacct tcaggccggt
     1201 gcaacgatca ttaacactgc ctcggtccag gcttacgacc cgtccgcaaa cctgctggat
     1261 tatgcgcaga cgaaggcgag cattgttgct tttacaaaat cactcgccaa gcaggttgcc
     1321 aagcagggca ttcgcgtgaa cgccgttgct ccgggcccgt tctggactcc cctgcagccg
     1381 agcggtggcc aaccgccgga agaaataccg gggtttggcg gaggcactcc acttgggcgc
     1441 cctggtcagc cggccgaact tggtccgctg tatgtgctgc ttgcttctca ggattcaagc
     1501 tacgcgaccg gacaagtgct tggcgaatcg ggcgggagcg gacagcccta ggttgctcga
     1561 tgactcctga gcgggagcag cctgaacagc gggagcaaga ggagactcgc cgtttgtcaa
     1621 ttcgtgtcga atgctgtgga ggctatcaag gagtccagga gcccgtcgct ttctggcttg
     1681 gtaatcggcg gctcaaagtt caggccttgg tagaccgctg gttcgcaccc gggcaatact
     1741 ggttcaaagt ggacgcagat gacggcaaca tctatgtgct caaatatgac gagaacgtgg
     1801 gcaattggga aatcgcggca tatcgttccg gtgcgcttcg ttagggcggc ggcgtcaaaa
     1861 ggaggtcgct gctgacactt tcccagcaac gttcgcggtt ttcattcctc caagatctcc
     1921 ttatttcgcc gtcccctcgc ttttctcatc caatcggttg ccggtagtgc ctgtaaaatc
     1981 ggcctgatgc atgccattcc ctactggcgc ctgtcgggct tttatttctt tcatttcgct
     2041 ctgatgggcg cgttcgtgcc ttattggagc ctctatctcc agtcgatttc ctttacggct
     2101 tttcagatag gcgttctgac ctcgctgttg caagggatgc gcgtttttgc tcccgccgca
     2161 tggggatggc tgtccgatca ctcaggcaag cgggtggcga tcgtgcaggc tgcggcagca
     2221 ggaggcttgt tgagctattg cgcagtgttt ttcggcactt cgttcggctg gctgttcgta
     2281 agcatggctc tgatgagttt tttctggagc gcgtctctcc ctctcatgga agcgacaacc
     2341 ctttcgtatc ttcgcgaggg tatcgcaaga tatggccgca tccgggtgtg gggttcggtt
     2401 gggtttatct gttcggtcgc tggaatgggc tacatgctgg atgctctcgg tactgcgtca
     2461 ttgttgtggg cggtgctggg tttcaacgtc ggcatcgtca tcttcacaag caagataccg
     2521 gaagcgggaa ggagcgtgca tcccgccgcc ggcctttctg tcagtgaaat tttcaagcgc
     2581 ccggaagtcg ccgctttttt tgttgcctgc atgctgatgg cgctggccca cgggccctat
     2641 tacacttttt attccattta tctcgttgag catggttata gcaagagcgc ggtcggctgg
     2701 ctctgggcgc taggagtcat atccgaggtg ggtgtatttt tcctgatgcc gcagcttgtg
     2761 cagcgattcc ggctcaaaca gatcatggtg ttcagtttta cctgtggggt tgcccgtttt
     2821 ctgatgatag gctggggcgt tgactcaccg tccatcgtgc tggcagcaca ggttcttcat
     2881 gcggccactt acggttcgca tcacgccacg gccatgctgc tggtccatca tttctttcct
     2941 gggcgccacc aggcgaaagg gcaggcgctt tatatcagtc tcacgttcgg gcttggaggt
     3001 gcgctgggcg gcttgctgag cggttataca tgggaaacgc tgggggctgg gctcacattc
     3061 accgtgagcg ccgccgcagc cctgacagga ttactgctga ttggctggaa gatgcaggtc
     3121 gatcacccga gtgatgaata tggtgatagg tagctgatcc ttttcgtttg tttcctgctg
     3181 cactgttatg ggataattgc cgattgactt cattcaaaga catgcaaacc ctatacgaca
     3241 aactttggca aagccatatc gtctacgagg agcctggatc gggctccatg gatgagcagc
     3301 ccaagcacga tcgcatggta ttgctttata tcgatcgcca cctggtgcac gaagtgacca
     3361 gtcctcaggc attcgaaggg ttgaagctgg cggggcgcaa accgtggcgg cgggaatcgg
     3421 ttctggctgt ggcagatcat aatgttccca ccacgggccg cagccagggc atcagtgatc
     3481 ctgtatcacg catccaggtg gagacgcttg atcagaattg cgaggaactg aacattaccg
     3541 aatttcggat gaatgacgtc cgccagggca ttgttcatgt cattggtcca gagcaggggg
     3601 ccacgctgcc aggtatgaca gtcgtgtgcg gcgattcgca taccagcacg catggggcct
     3661 tcggctgtct tgcttttggc attggcacat cggaggttga gcacgtgctg gcgacgcaat
     3721 gcctttcgac aaaaaaagca aagtcgatgc aggttctggt cgaaggcgac ctgggccatg
     3781 gcgtcaccgc caaggacgtt gcgcttgcga ttatcggaga aatcggcacg gcaggcggta
     3841 acggttatgc cattgaattt gcaggcggcg ccatacgcgc gctttcaatg gaaggcagga
     3901 tgacgctttg caatatggcg atcgaggcgg gcgcccgcgc gggtatggta gcagtggatg
     3961 atacgactat cgaatacatc aagggccggc cgttcgcacc caaaggggat ttatgggaaa
     4021 aggcggttgc ctactggcgc acgctgaaaa gcgacgaggg cgcgcgtttt gacaagatcg
     4081 tgcggctgga tgcgacgcag gtgaagccac aggttacgtg gggaacatcg ccggagatgg
     4141 tggttgccat cgacgggaag gtgcctgatc ctgcccagat cgagaacgtc gtgaagcggc
     4201 acgacgtgga gcgtgccctg aagtacatgg gactggcgcc caacacgccg atcagcgaaa
     4261 tcctgctgga caagatattc atcggctcct gcaccaattc ccgtattgaa gatctgaggg
     4321 ccgcggcaaa catcgtgaag ggcaggcgta tcgcgtccaa catcaagctc gccatggttg
     4381 tgcccggttc aggcttggtc aagcaacagg cggaacggga agggcttgac gcgatttttc
     4441 gcaacgcggg attcgagtgg cgcgaaccgg gttgctccat gtgcctggca atgaacgacg
     4501 accggctcga acccggcgag cgctgcgcgt cgacctccaa ccgtaacttt gaaggcaggc
     4561 agggccaggg aggacgaacc cacctggtaa gtccggcgat ggcggccgcg gccgcagtgg
     4621 caggccgttt tgttgatgct agtaaaatat actaaactgg aaatggtttt ccatgttgcg
     4681 taactaatga taattaaagg agattggcat gaaaacattt tccgcattga ttgccttgat
     4741 tgctgttttc cagttatccg cttgtaatac gataggcggg atggggaggg atatgcagaa
     4801 ggggggagaa gcggtcgaga gatcgtccaa atagccggtg gagaagtttc atccatgtga
     4861 agggttggtg gcaccgctcg atcgagcaaa catcgatacc gatgccatca tccccaagca
     4921 attcctgaaa tcgataaaac gatcgggttt cggccggaac ctgtttgatg agtggcgcta
     4981 cctggatcac ggcgagccgg ggatggattc ttcgcagcgc aagcccaatc ccgatttcgt
     5041 actcaacctg ccacgttatc gcggggcgca gatactgctt gcacgggcga atttcggctg
     5101 cggttcaagc cgcgaacacg ctccctgggc tatccaggat tacggcttca aggccattat
     5161 cgcccccagc tttgctgata ttttcttcaa caattgcttc aagatcggcc tgcttccgat
     5221 tgtgctcgat gtggacagtg tcgatcaact gttccacgag gtggaagcag ccgaaggcta
     5281 ccggttaaag gttgatctgc cggatcaagc ggtgaccaca cctggcggtc aacgtttctt
     5341 tttcgacatc gatcctttcc gcaaacatag ccttctcaat gggctggatg agatcggcct
     5401 gaccttgcag cacgcggacg agatcagggc attcgaagaa aagcatcgcg ctgcgcagcc
     5461 atggttgttt gcgtagaaat tacctgtagc gcgggaaaag catcgtgaaa atcgccatcc
     5521 tcgcgggcga cggcattggt ccggaaatcg tcgcgcaagc ggtgcgtgtt ctggaagcgt
     5581 tacgtaaaga cgggctaaaa attgaactcg aaccagccct gctgggcggt tgcgcagtag
     5641 acgccgaggg tgaaccattc cccgagtcca caagaaagct tgttgccgag gcagatgcag
     5701 tgctcctggg tgcggtgggc ggcccgaagt atgacagcct gccgcgcgcc ttgcggccgg
     5761 aaaaggggct gctgggcata cgcaagtttt tgaacctgtt tgccaatctg cgccccgcga
     5821 tactttatcc tgagcttgca aacgcatcca cgctcaaggc tgaggttgtg gctgggctcg
     5881 acgtccttat tgtgcgcgag ttgaccggag acatctattt cggcgagccc aggggtatcg
     5941 agcaccgcgg aggccagcgc gtgggtttca atacgatggt ttacagcgaa acggagatcg
     6001 ggcgaattgc cagggtagcc ttccaggcgg ctcgcaagcg cagccggcgg ttgtgctcag
     6061 tggacaagat gaatgtgctc gagtgtacgc aactgtggcg ggatgtggtg accgaaacag
     6121 cagaagaata tcccgacgtc gagctgtcgc acatgctggt cgacaacgcg gcaatgcagc
     6181 tgatacgcga cccgcggcaa ttcgacgtaa tggtgaccgg caacatgttc ggggatatcc
     6241 tgtctgatga agcgtcgatg ctgacaggtt cgatcggaat gctaccctcc gcgtcgctag
     6301 atcaacacaa taaggggctg tatgagccca tacacggctc tgctcccgat atcgcgggga
     6361 aaaatgtggc aaacccgctg gcaacgatac tgtcggccgc catgatgctg cgctatacct
     6421 ttgatcttga acccgccgcg gcacgtattg aaggcgcggt gaagagggtt ctggcgcaag
     6481 gctatcgcac ggccgatatt tatgaagaag gaatgaaaat ggtcggaacg gcggagatgg
     6541 gcgacgctgt cctggcaaat atttcggact agcagaggcg atcaatgaag cgagtagggt
     6601 ttatcggctg gcgtggtatg gtgggttcgg tgctcatgca gaggatgaag gaggaaagcg
     6661 atttcgctct ggttgagcca gcttttttca cgacttcgca aaagggcggc aaaggtccgg
     6721 atattggggt ggaggcgccg ccgctcaagg atgcccacga catcgaagag ctcaaggcta
     6781 tggatatcct catttcctgt cagggtgggg actataccag cgcgattttc ggaaagctcc
     6841 gcgagtcggg ttggcaagga tactggatcg atgcggcttc aacactacgg atgggcgacg
     6901 acgccgtcat catcctcgat ccagtcaata tgccagtgat caagcaggcc ctgcatgacg
     6961 acgtgaagaa tttcatcggc ggaaattgca cggtgagcct catgctgatg gcagtgggcg
     7021 gcttgtttga agcggatatg gtcgaatgga tgagcgcaat gacgtatcag gcggcttccg
     7081 gcgccggcgc gcaaaatatg cgggagctgc ttcttcagat gggggaagcc caccgcgtgg
     7141 cgaaggactt gctggacgat ccggcgtccg gcattcttga catcgaccgg caggtggccg
     7201 gcacgctgcg ggacggcaat tttccaaccg acaatttcgg tgtgccgctg gccggaagcc
     7261 ttattccctg gatagacaga gatcttggga acgggcaaac ccgggaagaa tggaaaggcc
     7321 agtccgaaac caacaagatc ctggggcggt ccgaccggat ggtgccggta gacggcatct
     7381 gcgtccgtgt gggcgcaatg cgttgccaca gccaggcgct taccatcaag cttaagcagg
     7441 atgtgccgct ggatgagatc aaggacattc tttccgcttc aaacaactgg gtgcgggtga
     7501 ttcccaacga gcgcgaaagc acgctcaagg aactgactcc tgctgcagtg acaggaaacc
     7561 ttgcaattcc cgtgggccgg ttgcgcaagt tggccatggg agaggaatat ctttccgcgt
     7621 tcacggtagg agaccagtta ttatggggtg cggccgaacc actgcgaaga atgctgcgaa
     7681 tcctggtgga ggcttgacgg cggcaatctg taccgttcga tcaaatagaa ttatgcgtga
     7741 atttgaatgc gcattctcgg gagcgattgg caggcccgat tcaccgtgca ttgcagatcg
     7801 gcatgcgatt ccttcctgaa aagcgatcat gttgaagcgc cgtagcgttt atttagtcgc
     7861 ctttgcgctt gcgtcgccgt ggatgatcga tgcagccggt gcggcgggac tgggcaggct
     7921 gactgtcgca tccatccagg gacaaccatt caaggccgaa atcgacctcg ttgccgtaaa
     7981 aaaggaggag aaatctcttt tgacggcaag gcttgcatcg caagaaggtt tccgccaggc
     8041 aagcgttgac tacctgcccg tcctttccac atttcaggca accatcgaaa cgcgcgccaa
     8101 tggagccccc tatgtgagga tcgtttcacc tcagtctgta agcgaaccgt tgctgaatat
     8161 tctcgtcgaa ctgaattggc cgtcggggcg attattgagg gaatataccg ttgtcctccc
     8221 tcattcggaa catgatggaa atccgatcgt aaagcaggcg cgcggtccga gccctgtcgt
     8281 ttcaactaaa gtcgaatcgg ctgccaataa aaaccgtgcg ggtttgaggc acgatacgaa
     8341 ccggccacga gtagctgaac cggtggctac ggttgcaaat tccgcgtctt atggtcccgt
     8401 cagggaggga gataccctcg taggaatcgt taaaaatatg accggggccc gcggagcaag
     8461 catgaatcaa acgctgattg ccttgcatcg agccaatccg gacgcattta tcggcgataa
     8521 catacacgag ttgaaggcag gcgccacgct ccgcataccc gatgggaacg agatcagtgc
     8581 gatagctccc gtcgaggccg acaggatcgt gcaactgcag acggccgaat ggcgtcaacg
     8641 caggctacca cttattgcaa gggctccaga agaattgaag cagaccgtaa ccggaacaat
     8701 cgagcgcact gccgaactaa acctcgtttc agcgaaagaa cccgccacag aagttttaag
     8761 actctcgaaa ggtgtggagc ctcggggcca ggagcaggta tcggcaaacc atgctgcaac
     8821 tcctggagag aaacagcatg cgaatgccca ggatcagtcc caggcaatgg aagaggatgc
     8881 gacagcgagg gaacgggcgc tgcgcgaggc caatgagcgc gtcgcgctgc ttgaaaaaaa
     8941 tatcaaggaa ttgcaacggc tgctggagtt gaagaatgtt cctcttgcgg agatgcagag
     9001 gaaagcggag cggatgcaac ccgacccaga gacactatcc cctgatgcct tacaccctca
     9061 ggcagcgcca gagagcgtgg cagacggagg agcggtagca gtggctcggc aatcatcgga
     9121 aaacgcggaa gcaactggaa ctaagcgaac cgattcgacg cgaaccacta cctccgaggc
     9181 caccatatcc gaccggatag agcttgccaa acctgccagg tcagcaaaat tgaatcatga
     9241 gatcgacctc tcgaatacgg aaacgggcag acgggggaaa gtctcattcc tcgatggtgt
     9301 gatggcgaat tttgaatacc tggggggtgc gcttgtactt ttattgacgg gtatagtggg
     9361 agtatccatg gtgcgcggct cgaagtcgtc atctgttttc gagtccgatt atgacgatgg
     9421 aactgactcg tccacccttc cgcagaagaa gggcgagtct ggcattgtca tgcgttccga
     9481 agcagacccg gcgggaccca gcgatgtgat ccatccacag gcttccccgc agtctcaccg
     9541 gaccggcatg agcctgacaa taccggagct gtgcctggaa ccggcagcga caccaacccc
     9601 gatgaaatgt gaatctgaag ggggtgccag cgtgagcctg gtagtggcag aggcgcgggt
     9661 cgaggcgacg acaaaaccac ttcccgtgga atatgaaacc gagcgggata ctaactggcc
     9721 cgaaatatta actaaactgg atctcgcgcg agcctaccag gaaatggacg acaaggagtc
     9781 cgcacgccag attctcgagg agatccttcg cgaaggtgac aagcgacagc gcgagcatgt
     9841 gattgcaata ctgaacaggt tgtagggcgt ggttttggtt gactcttcc
//
LOCUS       DASSHJ010000137         6999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1429074,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000137 DASSHJ010000000
VERSION     DASSHJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429074"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..819
                     /locus_tag="VFI43_05400"
     CDS             169..819
                     /locus_tag="VFI43_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET7061595.1"
                     /translation="MPPARPRREQTQAEEIANSISHGLGLVAALVGTPFLITHAVRHG
                     NIGFVVGVSLFSTTTIFLYLASTIYHALPPGKAKRVFRVLEHCAIFLLIAGTYTPFTL
                     GVLHGVWGWVLLSAIWGLATAGVVLKVFYGASLPILSTALYLFMGWMVVIAADLLVTK
                     MPTPGLLWLVAGGLSYTVGVIFFAIDSRLQYAHLIWHLFVLAGTTCHYFAVLWYAA"
     gene            1050..1253
                     /locus_tag="VFI43_05405"
     CDS             1050..1253
                     /locus_tag="VFI43_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7061596.1"
                     /translation="MATGIVKWFNDSKGFGFITPDDGGNDLFAHFSAIKGDGFKSLHE
                     SQRVTFDVTTGPKGQQASNISPN"
     gene            1556..2767
                     /locus_tag="VFI43_05410"
     CDS             1556..2767
                     /locus_tag="VFI43_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET7061597.1"
                     /translation="MKQYEVLLPNPDRTLDRITAMAARLFDVPVAVIGIVDAGHIRLV
                     SRWGLEANEIGFAPGYPTITMLRDAPYIVANTKDNTSPLTETLATEVAGMGFYAGAPL
                     ITAQGFNLGALWVIDRRPRILNEKEIASLQDLAYFVMERLESSLNLSRVVVQTIRNKD
                     AARKTSLEQSEVISSMGHELRTPLNTMLGFAQLMDMGNPPPTASQKINIEYILESGWY
                     LLSLINQILQSAAIESGKLSLSQTPLSLFEVLEECQTMIKFQAQKKHITLNFPRHEHD
                     HPVRVLADETKLKQVLINLLTNAIKYNQEMGNVTVEYSQNDVGTTRISIHDTGFGLTQ
                     DQLDKLFQPFNRLGRETGAEEGTGIGLVVTKKLVEFMGGEIGVKSVIGVGSTFWVDLP
                     SADPSKSKIDE"
     gene            complement(3111..4256)
                     /locus_tag="VFI43_05415"
     CDS             complement(3111..4256)
                     /locus_tag="VFI43_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7061598.1"
                     /translation="MKFAFKKLNQNLSLALGVLALTPLSASALVINSSTASFNNSASV
                     TDVEGGGATSKNGASLGTSTISKFNPNQGVLTGATLNLTSTRSQSTWVKSTTGGFIGS
                     NAGVTSTGTGSSTANISAPGVTSTFSSAISNSDTCAGTRLNACTGAATTSVATPTNLN
                     AIVSSANLDSYAGAGLVTVDRSAPTLTATQASMVFTGTESTEYAVTWSGQVSASYDYL
                     LHAAPSFNASSSMLTLDLDLGTFHIGDAATLGFDLFNLADANRVGLDLDGLTASGDVS
                     ALGTNLSLFSGLAQGSGINWLATLDTSTTGIFHTSYLLRMSDADVGAASTRFNYNLTL
                     NLSGRVDELPAEIPVQSALIGEVPEPSTLALLGIALTGLRFSRKSKS"
     gene            4755..5252
                     /locus_tag="VFI43_05420"
     CDS             4755..5252
                     /locus_tag="VFI43_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061599.1"
                     /translation="MFSRGDAVLDSFTQDQAAAAVDGASDRPHVRVIHSAVKGRARLE
                     VNRLYRCEPVRRKVESELLSDPRIIRVSANILTGRVLILFNPECTIDEIAGLIEAMMR
                     RSVPASGLQPSGKSRYPVFSSTAGATTDEGRVPQKQKRPMAGTARKRGIGSARDFKKL
                     WSYPG"
     gene            5317..6591
                     /locus_tag="VFI43_05425"
     CDS             5317..6591
                     /locus_tag="VFI43_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HET7061600.1"
                     /translation="MIHLLIHTFRPISPMDLSSGGNRIRGLATAMLIAMLAILVLSNV
                     FLSFHPSVGYVRAFAEAAVVGALADWFAITALFRQPLGLPIPHTAIVPRNKERIGEAL
                     GRFVELNFASPEVVSSKLAGVDMAGKLAKWLSDPRRTEVFADRLAKVVPQLLDAVDDG
                     NVRRFVSSSLADRAYQVNLGPLLGEAIRMLTAEKRHQVLLDKLLHEADEYITANEQRI
                     RERVRENTAWLWQRLYMDDKVADNVVAILREAFAEIGRDSGHPLRVRLDEAIAKLTLQ
                     LADSPEYREQIAAHTRRLLGHPSVQEYISEIWRNIRKSLREDTESDNSVLKSWLAEVI
                     QAASHALLHNEELRGRLNEWMHDMAVEAVQSHQRDVGLLIADTVRAWDTETVTRRIEQ
                     HVGEDLQYIRINGTLMGGLVGLAIYTVSRAFG"
BASE COUNT         1658 a         1728 c         1880 g         1733 t
ORIGIN      
        1 cgtggttgat taaaaggaat cttgctgggg gaattagcag gatgtaaaag ctttaaaacc
       61 cgccggctgg cgggtttttt atttgtgcac tcagcatggg cgcactcctg gggtgagtta
      121 ttagagagac aaaaagatca aaccgagcag catgtggagg ttctaatcat gccccccgcg
      181 agaccaaggc gtgagcaaac gcaagcggaa gaaattgcga acagcattag ccatgggctc
      241 ggtctcgtcg cggcactcgt gggaactccc tttctcatca cgcacgcagt acggcatgga
      301 aacattggat ttgttgttgg tgtgagtctt ttctccacga cgaccatatt tctttacctt
      361 gcttctacca tctaccacgc attgccccca ggcaaagcca agcgcgtatt tcgggtcctt
      421 gagcattgtg cgatctttct tcttatcgct ggtacatata cacccttcac gctaggcgta
      481 cttcatggcg tatggggctg ggttcttctt tcagctatat ggggtctcgc tacagccggc
      541 gtcgtcctga aggtgttcta tggggcgtcc ttgcccattc tatccaccgc tctctatttg
      601 ttcatggggt ggatggttgt gattgccgca gatctgttgg ttaccaaaat gccaacaccc
      661 ggcttgctct ggttggttgc gggagggctg tcttatacgg tcggagtaat tttttttgct
      721 atcgattccc gcttgcaata cgctcatcta atttggcacc tctttgtttt ggcgggcacc
      781 acatgccatt acttcgccgt gctctggtat gccgcctaac cttatccttt gccacgtaca
      841 cagcgatcga acgcgtcaat caatgtcttg ctgacaaaaa agttgtggcg gttattgtga
      901 aataatagtg tgcgcttcct cagatagcgt gtatcctacc aaggtctttt ggattcaagc
      961 cgtaaaacga tacctttggt tgaccatttc ccttgatttc aacgaccgcc tgcaaatggt
     1021 tacaggtgaa atcttattaa ggaaataaca tggcaacagg tattgtaaaa tggtttaacg
     1081 atagcaaagg ctttggcttt attacgccag atgatggggg aaatgatttg tttgcccact
     1141 tctctgcgat caagggtgat ggcttcaaat cgttgcacga gagccagcgc gtcaccttcg
     1201 atgtgactac tggtccgaag ggccaacagg catcgaatat cagtcccaac taaaatcagt
     1261 aaaagcaacc aaaattcctc atcggaattt tggttcgaga tctgacacag tccccagcgg
     1321 ggggaggttg cgtgttgatt tctcaacccc cgaactccgt tccatttaca gttattctat
     1381 tgccgcactc cgcccggagt aaattgtgcc ttggttacgc cataggccat gtcacccgct
     1441 ttcttttgcc cccacctaca aagatttcct gcatgattcc ctgcgtctac actcgcggag
     1501 gccaaaaatt aatcgattaa atatgcccgt gaatgaagca gcgcggctgc tcgtattgaa
     1561 acaatacgag gtattgttac ccaatcccga ccgaactcta gatcgaatca ccgcgatggc
     1621 agcacgcctg tttgacgtcc cggtcgcagt aatcggcatt gtcgatgccg gtcatatccg
     1681 gcttgtatcc agatggggcc ttgaagccaa cgaaatcggg tttgccccgg gttacccgac
     1741 aattaccatg ctacgtgatg ctccttatat tgttgctaat acaaaggata ataccagccc
     1801 gcttacagaa acgcttgcaa ccgaggtagc gggtatgggg ttttatgcag gagctccgtt
     1861 gattactgct caaggtttta atctgggagc gttatgggtt atcgatcgaa ggcctaggat
     1921 attgaatgag aaagaaattg cgagcttgca ggatttggcg tatttcgtga tggaacgatt
     1981 ggaatcaagt ttaaatttga gccgagtagt ggtacagaca atccgaaaca aagatgccgc
     2041 gagaaaaacc agtttggagc aatcggaagt catttccagt atgggccatg aactgcgaac
     2101 gccgctaaac accatgctcg gatttgctca actcatggac atgggtaatc cacccccaac
     2161 agcatcccaa aagataaata ttgaatatat tcttgaatcg ggctggtacc tgctgagcct
     2221 gattaatcag atccttcaat ccgcagcaat cgaatcgggg aagttatcgc tgtcgcaaac
     2281 gccattatcg ttgttcgaag tattggaaga gtgccaaacg atgattaaat tccaagcaca
     2341 gaaaaagcat atcacattga attttcctcg acatgaacat gatcatcccg tgcgtgtact
     2401 ggccgatgag accaaattga agcaggtcct gatcaatctt ctcaccaatg cgatcaaata
     2461 taaccaggag atgggcaacg taaccgttga gtattctcaa aatgacgtag ggaccactcg
     2521 gatcagtatt catgacaccg gctttggatt gacgcaagac cagctcgaca agttgtttca
     2581 gccgttcaat cgacttgggc gtgagaccgg cgccgaggaa ggaactggca tcgggctggt
     2641 cgttaccaaa aaactggttg aatttatggg aggagaaatc ggcgtgaaaa gcgttatcgg
     2701 cgtcggaagc acattctggg ttgacctgcc atcagccgac cccagcaaat cgaaaatcga
     2761 tgaatagtgg ggcaataaac agactatggg agcatgtttg gcaaccgaat gactttcttg
     2821 ggaaatcatc acggttggaa taagcaaaca acttttcgtg gcgttgataa tcctcactgc
     2881 gtgcctgcaa tcgggatgaa gatcgaagtt atccaggccg gtttcggaga atcaggaact
     2941 cgcttgaaac gagttcctga ttctgccagg gggcagctgg atagctgaac catcgggaaa
     3001 aatattgctt ttacgagccc aggcaagaat tgaaggcggg cggcaggttc ctcatccaag
     3061 caagtgcgct tcgtctcttt gtctgagaaa ggatgataat cctgctgcct ttatgatttg
     3121 cttttgcggc tgaaacgcag ccctgtcaat gcgatcccta gaagagcaag agttgaaggt
     3181 tccggcacct cgcctatcag ggcgctttga acgggtatct cagccggtag ctcatcaacc
     3241 ctccccgata gattaagggt aaggttatag ttgaagcggg ttgaggctgc gccaacatcc
     3301 gcatccgaca ttctcagcaa gtagctcgtg tggaaaatgc cggtggtgga tgtatcgagt
     3361 gtagccagcc aattaatgcc tgagccttgg gccaatccac taaagagact caggttggtt
     3421 cccagagcac tgacatcgcc gctcgcggtc agaccatcga gatcgagacc tacacggttg
     3481 gcatcggcca gattgaagag atcgaagcca agagttgcag catcgccgat gtgaaacgtg
     3541 ccgagatcca ggtccagggt tagcatcgag gaagaagcat tgaaagacgg cgcggcatgc
     3601 agaaggtaat catagctcgc gctgacctgc ccggaccagg tgaccgcata ctccgtcgat
     3661 tccgtgccgg taaataccat acttgcctgg gttgcagtca gggtcggggc gctgcggtcg
     3721 acggtgacca agccagcacc ggcataactg tcaagattgg cggatgagac aatcgcattc
     3781 aggttggtgg gcgtcgctac gctggtggta gcagcgccgg tgcaggcatt cagacgggtc
     3841 ccggcgcacg tgtcgctatt gctgatcgct gatgaaaacg tgctggtgac gcccggcgcc
     3901 gaaatattgg ctgtgctgct gccagtccct gtcgaggtaa cccccgcgtt acttccgata
     3961 aagccgccgg tcgtggattt tacccatgtg ctttggctcc gtgttgacgt aaggttcagc
     4021 gtcgcgcctg tcaatacgcc ctgattcggg ttgaacttgc tgatagtgct cgtcccgagg
     4081 ctcgcaccgt tcttgctggt cgcacccccg ccttccacgt cggtgacact tgccgagttg
     4141 ttgaagctgg cggtggagct attgatgaca agcgcgctgg cggacagggg ggttaatgcc
     4201 aaaacaccta acgcaagaga aagattctgg ttaagctttt taaatgcaaa cttcatttca
     4261 ttcccataaa tgattgatat agaggactct gttgcagagc ttctccccag ttaaagcact
     4321 tatcgtgcca acaaaaaatt tcttcgttat tcaccagctt gtaatatccg gggacagaaa
     4381 ctcaagcgaa atgtaagggc cttcgacacg ttatggcttt attaccctgg atgcggggaa
     4441 agataatgaa atgcggttgg ggtgggatcg ggagatcgga gcgcacctcg cgttcgtttt
     4501 cccatcctcc gatttgaaga gataccttgc ctactccaac cccggcctga cacaagaatc
     4561 gccttgatca gtggtcagtc ctgggctaca acaagatatg gatctttgga ctcgatgcgg
     4621 agttgtgaat tgctcagatg tatttgcaga ccgcttctct cctaacagga cggtagcttg
     4681 cgcttgctgt tccacctgat cgcttccaat tatcgcggac cactataact aaccgaattc
     4741 gctccgcatc ccaattgttc agccgcggtg atgcagtcct tgattccttc actcaggacc
     4801 aagctgcggc agcggttgat ggggcaagcg accgccctca cgtccgtgtc attcattcag
     4861 ccgtgaaggg gcgagcaagg ctggaagtaa accgcctcta ccggtgtgag cctgtccgac
     4921 gcaaggtcga gtcggagttg ctatccgacc cccggatcat ccgggtgagt gcaaacatcc
     4981 tcacgggccg cgtattgatt cttttcaacc cggagtgcac gattgacgag atcgcaggct
     5041 taatcgaggc gatgatgcgc aggtcggttc ccgcctctgg tctgcagccg tctggtaaaa
     5101 gtcgttaccc ggtgttttca tccacggcag gagctaccac tgatgaagga cgcgtccctc
     5161 agaaacagaa acgtccaatg gcaggcactg ccaggaagcg cggtattgga agcgctcgag
     5221 acttcaaaaa gctctggtct tacccaggct aaagtgcgct ttcagcttac aattgaaaaa
     5281 caaaagcatg ggtagacggg acgtggcgtc cttctattga tccatcttct tatccacacc
     5341 ttcaggccca tcagtcccat ggatttgtct tccgggggaa accggattcg ggggctcgcg
     5401 acggctatgc ttatcgcgat gcttgccatt ctggttcttt ccaatgtttt tctttcattt
     5461 catccgtcag tcggttatgt gcgcgctttt gcggaagcag cggtggtggg tgcgctggct
     5521 gactggtttg cgatcaccgc tctgtttcgc cagccgcttg gtctgccaat tcctcacact
     5581 gcaatcgttc cgcggaacaa ggagcgtatc ggcgaagcat tggggcgctt cgtcgagctt
     5641 aatttcgctt ccccagaggt cgtctcatcc aaattggccg gggtggatat ggcgggcaaa
     5701 ctggcaaagt ggttgtcaga cccgcggcga accgaggtgt ttgccgaccg tttggccaaa
     5761 gtagtaccac aactgctgga cgcggtcgat gacggtaatg tacggcgctt cgtttcgagc
     5821 agcttggcag acagggcgta ccaggtaaac ctcggcccgt tgctcggcga ggcgatcagg
     5881 atgctcaccg cggagaagag acatcaggtg ttattggata agctgttgca tgaagctgac
     5941 gaatacatca cggcaaatga acagcgcatc cgcgagcgtg tgcgcgagaa tacagcctgg
     6001 ctgtggcaac ggctttacat ggacgataaa gtggcagaca acgtggttgc aatactgcgt
     6061 gaggcattcg cggagatagg acgtgattcc gggcacccgc tccgcgtgag gcttgacgag
     6121 gctattgcca agctgacctt gcagttggcg gactcgccgg agtatcgcga gcagatcgcc
     6181 gcacatacac gaaggctgct ggggcatccc tcggtgcagg agtatatttc agagatctgg
     6241 cgcaatattc gcaagagtct tcgggaagat acggaaagcg acaattcggt gctcaagtcg
     6301 tggttggcag aggtgataca agctgcttca cacgcattgc tgcataacga ggagttgcgc
     6361 ggccggctta acgagtggat gcatgatatg gcggtggagg cagtgcagtc tcaccagcgg
     6421 gacgtcgggt tgctgatagc ggatacggtt cgcgcgtggg acaccgagac agtgacaagg
     6481 cggatcgagc agcatgtggg tgaagatctt cagtatattc gcatcaatgg taccttgatg
     6541 ggtgggctgg taggactggc aatctatact gtatcaaggg ccttcggctg atatttccct
     6601 cgctggtttg ctccgtcagc tcgcccctga ttccatatcc aagttcgagg attcccgccg
     6661 aagagataga cctcctgttc cagccattca tcttccactc catccaatgc gattattgaa
     6721 ttatttttca ggaatccgag aacttgctgc attccatcac gtcgaagtaa ccgtgggagg
     6781 aattatttta ttctgtcgct ccgctcacac aacgttcttt aatgaagaga tgtttttgaa
     6841 gatgttgctg accaagtcgc aaatagcgtt gcggttatta aagccaatca tattcttgag
     6901 catttaatct tgaaggaggt aatcaatcat ggaagcagta attttagtga tcggcgtagg
     6961 cgcagcagcc ctctggtttt tcatgtccaa ggataaacc
//
LOCUS       DASSHJ010000138         8851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_681358, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000138 DASSHJ010000000
VERSION     DASSHJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681358"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /gene="dnaK"
                     /locus_tag="VFI43_05430"
     CDS             <1..1519
                     /gene="dnaK"
                     /locus_tag="VFI43_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7061601.1"
                     /translation="VITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAAALACGMD
                     KKDGDRKIAVYDLGGGTFDISIIEIAEVEGEHQFEVLATNGDTFLGGEDFDSRIIEYL
                     VDEFKKENGIDLKKDMLALQRLKDAAEKAKIELSSSQQTEVNLPYITADASGPKHLAV
                     RITRAKLESLVEELIERTVEPCRIAIKDAGIKVSDIDDVILVGGQTRMPKVQDKVKEI
                     FGKDPRKDVNPDEAVAMGAAIQGGVLQGSVKDVLLLDVTPLSLGIETLGGVMTKLIQK
                     NTTIPTKAQQVFSTADDNQTAVTIHVLQGEREMASGNKSLGQFNLTDIPSAPRGLPQI
                     EVTFDIDSNGILHVSAKDKATGKENKIKIQASSGLSDEEVQRMVKDAEAHAEEDHKAM
                     ELVTSRNQCDAMIHSVRKTLKEHGDKLGGDEKSKIEAALKDAEEALKSDNKDVIEAKT
                     QALAEVSHKLAEKMYTQEQPNEGQPGGQAGAQGGGEKPVEGEVVDAEFEEVKNKK"
     gene            1654..2760
                     /gene="dnaJ"
                     /locus_tag="VFI43_05435"
     CDS             1654..2760
                     /gene="dnaJ"
                     /locus_tag="VFI43_05435"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7061602.1"
                     /translation="MSKRDYYEVLGVNRDSDEEDIKKAYRKLAMKYHPDRNPDNPKTE
                     EHFKEAKEAYEILSDPRTRAAYDQHGHAGVDAGMGGGAHGFTDAFSDIFSDLFNTSRG
                     HSNVHRGADLRYNLEISLEQAARGTETKIRIPTMEVCETCHGSGARPGTSPITCPTCN
                     GHGQVRMQQGFFSIQQTCPKCHGSGKFISSPCATCAGSGRVRQHKTLSVKIPAGVDEG
                     DRIRLSGEGEAGVNGGPPGDLYVVIHLAPHSVFQRDHNDLHCEMPISFTTAALGGEIE
                     IPTLDGHAKIRIPAETQSGKVFRLRGKGIKGVRSNTPGDLLCHVVVETPVKLTGRQRE
                     LLQELEESMNQEDGSRHSPRAKSWMDKVKAFFAE"
     gene            complement(2951..3508)
                     /locus_tag="VFI43_05440"
     CDS             complement(2951..3508)
                     /locus_tag="VFI43_05440"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7061603.1"
                     /translation="MTKPPLDLTETTVSSLNVYDGDLLHVRADRARLPDGKISVREYI
                     THPGAVVIIPLLANSELVMERQHRYPLHRDFYELPAGKIDGGEDPLLCAQRELLEETG
                     YSAESWRYIATSHPCIGYSNEKLVYYLAQELTFSSANLDDGELLEVFTLSLAEALEWI
                     RTGKITDNKTVCGLFWAEKLLSGQW"
     gene            complement(3586..4959)
                     /gene="phoR"
                     /locus_tag="VFI43_05445"
     CDS             complement(3586..4959)
                     /gene="phoR"
                     /locus_tag="VFI43_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HET7061604.1"
                     /translation="MSGFWDRFAIIVPTVVVAGILLALLGPVVTLSLLSGVLLLIVVH
                     YQRNLYVLDKWLRGQSKSLPDRSGQWGDVFARLARQMREQKHSHQQISSAFERLRRAT
                     SAMPEGVVILDEVDRIEWCNPIAEKHLGINLNLDSGQHITHLLRQTQFTQYLSAHNYS
                     EPLTIRQPRSTDLTLSLQLVPYGDRQKLLLSRDVTRLEEIQTMRKDFVANVSHELRTP
                     LTVIGGFLETLTDESNSDPETRRWALALMSDQTTRMQRLVEDLLTLSRLEDTENLARE
                     DRVDIPDMLRKLYDEAQSLSGGRHHIILDLNSSAWLLGRTDELRSVFSNLISNAIRYT
                     PESGAITLSWSTDNDQSVFSARDSGIGIEPMHISRLTERFYRVDRGRSRETGGTGLGL
                     AIVKHVLTCHQAKLDIESQPGRGSCFSARFPAERVIMPTDSDVVNGKTGPETSSSDGT
                     SSFDGRV"
     gene            complement(4998..5705)
                     /gene="phoB"
                     /locus_tag="VFI43_05450"
     CDS             complement(4998..5705)
                     /gene="phoB"
                     /locus_tag="VFI43_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HET7061605.1"
                     /translation="MAATILVVEDEPAIQELIGFSLRQARHTVFCARNAEEAMEIINE
                     ALPNLVLLDWMLPGMSGMEFARMLRRNTRTKAIPIIMLTARAEESDKVAGLETGADDY
                     MTKPFSPRELLARIKAVLRRRAPEAADDIVEIGGLRLDSATHRVTAEDSEVILGPTEF
                     RLLQFLMTHSERVHTRSQLLDQVWGDHVFVEDRTVDVHIRRLRKALEPVNKDGLVQTV
                     RGSGYRLSAEQTAHFEQ"
     gene            complement(5846..7150)
                     /locus_tag="VFI43_05455"
     CDS             complement(5846..7150)
                     /locus_tag="VFI43_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET7061606.1"
                     /translation="MNVGDKARGLLLESFHTALAAADPLRVMPEHLLHGLPHVSGRIL
                     VVGAGKAAASMALAVETHLLRGTKNDPVLDGIVLTRYGHGLPLKKIKVVEAGHPLPDE
                     HGEQATRAILEEVRKLRHEDFLLCLMSGGGSSLMSLPIDSVPIADLRKLTSELLRSGA
                     EIHEINIVRKHLSKILGGRLAASSGAPVLALIISDVAGDDPTDIASGPCAPDPTTYSD
                     ALEILDRYEIKASYAVMETLQDGILGRLPETPKPGEPVLQHVENRVIATARDALMGAK
                     EFVRNQGIPAVIMSDSVTGEAREVAKVMTAVAKEIHRYAQPWKPPVVLIAGGETTVTV
                     KRNGRGGRNTEFLLSLAIELNGLEGVYALAGDTDGIDGTEDNAGALVTPDTLSRAKQL
                     GLKPPTLLADNNSYEFFEKLGDLIVTGPTRTNVNDFRAILIG"
     gene            complement(7165..>8851)
                     /gene="uvrA"
                     /locus_tag="VFI43_05460"
     CDS             complement(7165..>8851)
                     /gene="uvrA"
                     /locus_tag="VFI43_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7061607.1"
                     /translation="TESVTVREELGKYLNSQVCPECVGTRLRLEARNVRVGGLAIYEI
                     NALPLKEAKAFFENVELTGHKLAIAEKIIKEIASRISFLNNVGLDYLSLERSAETLSG
                     GESQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNGRLLETLKSLRDLSNSVIVVEHD
                     QDAILSADHVVDMGPGAGEHGGAIIAQGTPADIQEDPDSLTGKYLSGTLAIEMPEKRT
                     EPKMDRWLKITGASGNNLKNVNLNLPLGLFVCVTGVSGSGKSTLINETLYNAMAHYLY
                     GSTAEPAPYQTIEGLGFFDKVINMDQSPIGRTPRSNPATYTGLFTPIRELFAGVPQAR
                     ERGYGPGRFSFNVRGGRCEACQGDGMIKVEMHFLPDIYVTCDVCHGKRYNRETLEILY
                     KGKNINDILQMTVEQAHEFFSPVPVVARKLQTLLDVGLGYINLGQSATTLSGGEAQRV
                     KLSLELSKRDTGRTLYILDEPTTGLHFQDIDLLLKVLHRLRDHGNTVVVIEHNLDVIK
                     TADWIIDLGPEGGDGGGEIIAEGTPEEISANAKSFTGHYLQAMLRSRRDQLLQ"
BASE COUNT         2062 a         2424 c         2431 g         1934 t
ORIGIN      
        1 ggtcattacc gtcccggctt attttaacga ttcgcaacgc caggcaacga aggatgcggg
       61 gcggattgcc ggcttggaag tcaaacggat catcaacgag cccactgctg ccgcgcttgc
      121 ctgtgggatg gataaaaagg acggggaccg caagatcgcc gtatacgacc tgggtggggg
      181 gacgttcgac atttccatca tcgagatcgc ggaggtcgag ggtgagcacc agttcgaagt
      241 actggccaca aatggtgata ctttccttgg cggagaagat tttgactcgc gcattatcga
      301 atatctcgtg gacgaattca aaaaggagaa cggtatagac ctcaagaagg atatgctcgc
      361 cctgcagcgt ttgaaagatg cggccgaaaa ggcaaaaatc gagctttcat ccagccagca
      421 gaccgaggtc aaccttccct acatcactgc ggacgcttcc ggccccaaac atctcgcggt
      481 caggataacc agagcaaagc tggaaagcct cgtagaggaa ttgattgaac ggacagtgga
      541 gccatgtcgt atcgccatca aggatgcagg cataaaagtt tccgatattg atgacgttat
      601 tttggtaggc gggcaaacgc gcatgcctaa ggtacaggat aaggtcaagg aaatttttgg
      661 aaaagatccc cggaaggacg tcaacccgga tgaggctgtc gcaatgggtg ctgcaattca
      721 gggcggtgtg ctgcaaggct cagtgaagga cgtgctgctt cttgacgtca ctccgttgtc
      781 actgggcatc gaaaccctgg gtggcgtgat gaccaagctt atccagaaaa ataccacgat
      841 tccgaccaag gctcagcaag tgttttcgac ggcagatgac aaccagaccg ccgtgacgat
      901 tcacgtgcta caaggcgaac gcgaaatggc atccgggaac aagagcctgg gacaattcaa
      961 cctgaccgac attccttccg cgccccgcgg tctaccccag atcgaggtta ccttcgatat
     1021 cgattcgaac ggaatattgc atgtatccgc gaaggacaag gccacgggga aggagaacaa
     1081 gataaagatc caggcaagtt cgggcctttc cgatgaggaa gtgcagcgca tggtcaagga
     1141 cgccgaggcc catgccgagg aagaccacaa agcgatggag ctcgtcacca gccgcaacca
     1201 gtgcgatgca atgattcact cagtgaggaa gaccttgaag gagcacggtg acaagctggg
     1261 gggggatgag aaatcaaaaa tagaagcagc gctgaaggac gctgaagagg cgctcaaatc
     1321 agataacaag gatgtgatcg aagcgaaaac ccaggctttg gccgaagttt cgcacaagct
     1381 cgcagaaaag atgtatacgc aggagcaacc caatgagggc cagccggggg ggcaggcggg
     1441 tgcacagggc ggaggggaaa aacctgtaga gggtgaagtt gtcgatgcgg aattcgagga
     1501 agtaaagaac aagaaataag gatggggaag tgaacaggga tcaagcgcag aggaggcggg
     1561 gaacttgagt ttccgcctcc tctgccttgg cttgcaggaa gcagcgtgac gcgtgagtga
     1621 tctcctgctc ttcgagactt gaacaaaaca tatatgagta aacgggacta ctacgaggta
     1681 ctgggcgtca accgcgacag cgatgaggaa gatatcaaga aggcctatcg caagctggca
     1741 atgaaatatc acccggaccg caatccggac aacccaaaaa cagaggagca tttcaaggaa
     1801 gccaaggaag cctatgaaat actctccgat cctcgcacgc gggccgcgta tgaccagcac
     1861 gggcatgccg gcgtagacgc aggcatggga ggaggtgcgc acggctttac cgatgcgttc
     1921 agcgatatat tcagcgacct tttcaatacg agcagagggc attccaatgt tcatcgcggc
     1981 gctgatttgc gctacaacct ggagatctcg ctcgaacagg cggcaagggg aaccgaaacc
     2041 aagattcgca tccctacgat ggaagtctgc gaaacctgcc acggaagcgg agctcgtccg
     2101 ggaacatctc ccatcacttg ccccacttgc aacgggcacg gacaggtccg catgcagcaa
     2161 ggttttttct cgatccagca gacatgtccc aagtgtcacg gcagtggaaa atttatttca
     2221 agcccttgcg cgacctgtgc aggttcagga cgagtacgtc agcacaagac gctttcggta
     2281 aaaattcccg ccggggtgga tgaaggcgat cgcatccgcc tctccgggga gggcgaagcc
     2341 ggggtcaacg ggggacctcc cggagacttg tatgtcgtga ttcacctggc gccccatagc
     2401 gttttccagc gggaccacaa cgatttgcac tgtgaaatgc cgataagttt tacgacggca
     2461 gctctgggag gtgaaattga aattccaacg ctcgacgggc acgcgaaaat caggataccg
     2521 gcggagacac agagcggaaa ggtttttcgc ctgaggggca aagggatcaa aggggtgcgc
     2581 agcaatacgc ccggcgacct gttatgtcac gtggttgtgg aaacaccggt caagctgacg
     2641 ggacggcaga gggagctttt gcaggaactg gaagaatcga tgaaccagga agacggttcg
     2701 cgccacagcc cgcgggcaaa gtcctggatg gacaaggtta aggcattttt tgcggagtga
     2761 gatctcttcg tggaaatatt cttggttcag cgctttgtat tagtggtacc tcgctgtctc
     2821 acaacgagtc ccagttcgta ttagggatga gaacaaggcg gcaaggatga ggtgaagaag
     2881 acagtacatg cagtacggca aggagtcgaa gacgaagcca acgcagttat cgccctaata
     2941 cgaactggga tcaccactgt ccgctcaaaa gtttttccgc ccaaaacaat ccgcagaccg
     3001 ttttattgtc ggtaatcttt cctgtcctga tccattcgag cgcttccgca agcgataagg
     3061 tgaacacctc cagcagttcg ccgtcatcca gatttgcaga ggagaaagta agttcctggg
     3121 ccaggtagta gacgagtttc tcattcgagt aaccaataca gggatgtgac gttgcaatat
     3181 aacgccagct ctcagccgaa taacccgtct cctccagcaa ctcgcgctga gcacacagga
     3241 gagggtcttc gcctccgtca atttttcctg ccggcaattc gtagaaatcg cggtgaagcg
     3301 gatagcggtg ctgccgctcc atcaccagct cgctgttagc gagcaaaggt atgataacga
     3361 ccgcaccggg gtgagtgatg tattccctga cggaaatctt gccatccggc aggcgcgcgc
     3421 ggtccgcgcg gacatgcagg agatccccat catacacatt gaggctgctg accgtcgttt
     3481 cggtaagatc gagaggtggc ttggtcatca aatatttggg gccggaacac aggtcggaac
     3541 tcaggcggaa cataagcggt tcgtcgcaac cgaccatctt gcagattata cacgtccgtc
     3601 gaacgaactc gttccgtcgg atgagcttgt ttccggtccc gtctttccgt taaccacatc
     3661 gctatccgtc ggcatgatca cccgctctgc cggaaaccgg gcgctgaaac agctgcctct
     3721 gcctggttgg ctttcaatgt cgagcttcgc ctggtggcat gtaagcacat gcttgacaat
     3781 cgcaagccca agtcccgtgc ctccagtttc gcgcgaacgg ccgcggtcca ctctgtaaaa
     3841 acgctcagtc aggcgggaaa tatgcatggg ttcaatcccg atgccgctgt ccctcgcaga
     3901 aaacacactc tggtcattat cagtggacca gcttaaggtt attgccccgc tctccggcgt
     3961 gtatcggatc gcattgctga tcagattgct gaaaacacta cgcagttcat cggttctccc
     4021 caaaagccag gcgctgctat tcaggtcgag gatgatgtga tggcgtccgc cactcaatga
     4081 ctgcgcctca tcatacagtt tacgcaacat gtccgggata tcgaccctgt cctcacgagc
     4141 gagattttcc gtgtcttcga gccgggacaa ggtcaacagg tcttccacca gccgctgcat
     4201 gcgggtcgtt tgatcgctca tcagcgcaag cgcccacctg cgggtttccg gatccgaatt
     4261 cgattcatcg gtgagtgtct ccagaaatcc gccaatgaca gtcagcggcg tgcgcaactc
     4321 gtgcgaaaca ttcgccacaa aatccttgcg catggtttga atttcctcaa gacgggtgac
     4381 gtcacggctg agcagcagct tctgcctgtc accgtaaggc accaactgga gtgaaagcgt
     4441 caaatccgtg ctgcgtggct gcctgatggt caaaggctcg ctatagttat gggccgacag
     4501 atactgcgta aactgggtct gccgcaacag gtgggtgata tgctggccgc tatccagatt
     4561 caagttgatg cccaggtgct tttccgcgat ggggttgcac cactctatgc gatccacttc
     4621 gtcaagaatg acgacgcctt ccggcatggc ggaggtcgca cggcgcaaac gttcaaatgc
     4681 ggaactgatc tgctggtggc tgtgcttctg ctcccgcatc tggcgggcaa gccttgcgaa
     4741 cacatcaccc cattgccccg aacgatccgg taatgattta ctctgtccac gcagccactt
     4801 atccagcaca tacaggttgc gttgataatg gacaacgatc agcaggagca ctccgctgag
     4861 cagggatagc gtgaccaccg ggcccaacaa ggccagcagg atgcccgcta cgacaactgt
     4921 cggaacaatg attgcgaagc ggtcccagaa acccgacact tgttgcctta tcctggacga
     4981 agtgattgga tcatctgcta ttgctcgaag tgtgccgttt gttccgccga cagacgatag
     5041 cccgatccgc gcaccgtctg caccaatcca tccttgttga ccggttccag ggccttgcgc
     5101 agtctacgta tatgcacgtc gaccgtgcga tcctcgacaa acacgtgatc gccccaaacc
     5161 tgatcgagca attgcgagcg agtatggacg cgttccgaat gggtcatcag aaattgcagg
     5221 aggcggaatt ccgtgggacc cagaatgact tcactatcct ccgctgtcac gcgatgcgta
     5281 gccgaatcaa gccgcagccc gcctatctcg acaatgtcat cagcagcttc cggtgctcgc
     5341 cgccgcagca ccgccttgat cctggcgagc agttcgcggg gagaaaacgg cttggtcata
     5401 tagtcgtccg cgccggtttc cagtcctgcc accttgtcac tttcctcggc tcttgctgta
     5461 agcatgatga tgggtatggc tttggtacgc gtgttacgcc gcaacatcct cgcaaactcc
     5521 atgccgctca ttccaggcag catccagtcg agcagcacga ggttcggcag agcctcgttg
     5581 atgatttcca tcgcttcttc cgcattccgt gcgcaaaaaa cagtgtgccg cgcctgccga
     5641 aggctgaaac caatcagttc ctgaatcgca ggttcatctt ccaccaccag tattgttgcg
     5701 gccattcgca ttatcccttt aatcgctgct ccttctcgcc acctctttct ccggacgcac
     5761 ttccagtaat ctttttatca tattttccga atgttgcgat ttcatgacac gctatgcttg
     5821 atcaagtatc gcgaaaacgc cgtattcatc cgataagtat tgcccggaag tcgtttacgt
     5881 ttgtgcgcgt tgggccggta acaatcagat cgccgagttt ttcaaaaaat tcgtaagaat
     5941 tattatctgc aagcagtgtg ggtggtttga gtcccaattg ctttgcacgg ctcagcgtat
     6001 ccggggtaac cagcgcacct gcgttgtctt ccgtaccatc gatgccatcg gtatcaccgg
     6061 cgagcgcgta gacgccttcc agcccattca attcgatcgc aagcgacaga agaaattccg
     6121 tattgcggcc accccgtcca ttcctcttca cggtaactgt tgtttcgccc cctgcaatga
     6181 gcacgaccgg gggtttccac ggctgcgcgt agcgatggat ttccttcgca actgcggtca
     6241 ttaccttggc gacttcgcgt gcctcacctg ttaccgaatc gctcattatc accgcaggta
     6301 tgccctggtt tcgaacaaat tcttttgcgc ccataagcgc atctcgagcg gtggcaataa
     6361 cccggttttc cacgtgctgc aataccggtt cgcctggttt gggagtttca ggaagtctgc
     6421 cgagaatacc atcctgcaga gtctccatta ctgcgtatga cgcctttatc tcgtagcggt
     6481 caagaatttc caatgcatcc gagtaagtgg ttggatcggg agcgcacggt ccggacgcaa
     6541 tatcagttgg gtcgtccccc gccacatccg agatgatcaa tgcgagcacc ggtgctccgg
     6601 aggacgcagc gagtcggccc cccagaatct tggaaagatg ttttctgaca atattgattt
     6661 catgaatctc agcgcccgaa cggagcaact ccgacgtcag ttttcttaga tcagcgatgg
     6721 gaacactatc aatcggcaac gacatcaggc tggagcctcc gccgctcatg agacacaaaa
     6781 gaaaatcctc gtgccgcagt ttccgcactt cctccaggat cgctcgtgtt gcctgttcgc
     6841 catgttcgtc cggcagcgga tgtccggctt cgaccacttt tattttttta agcggcagtc
     6901 catgcccgta gcgggtaagg actattccat ccaggacggg gtcgtttttc gtgccgcgaa
     6961 gcagatgtgt ttcgactgcc aacgccatcg aggcagccgc ctttccagcg cctactacaa
     7021 gaatgcggcc tgaaacatga ggcaagccat gcaatagatg ctcgggcatg acgcgcagag
     7081 gatcggctgc cgcgagcgcg gtgtgaaagc tctcaagcag aagtcctcgt gccttgtctc
     7141 ctacgttcat ttcacccggg gctttcactg tagcagttga tcacgtcggg aacgcaacat
     7201 tgcctggaga taatgaccgg taaaactttt cgcattcgct gaaatttcct ctggggttcc
     7261 ttcggcaatg atttcgcctc ctccatcgcc accctctggt ccaagatcga taatccagtc
     7321 ggctgtcttg atcacgtcca ggttatgctc gatgaccact accgtattgc catggtcgcg
     7381 caggcgatgc agcactttca gaagcaggtc gatatcctgg aaatggagtc ccgtggtggg
     7441 ttcatcgaga atgtagagcg tacggcctgt atcccgcttt gaaagttcga gagaaagctt
     7501 cacccgctgc gcttctcccc ccgacaaggt ggtcgcggat tgaccaagat tgatatagcc
     7561 aagcccgaca tccagcaggg tttgcagctt gcgggccacg acggggacag ggctgaagaa
     7621 ttcatgcgcc tgttccactg tcatctggag aatatcattg atgttttttc ccttatacaa
     7681 aatctccaga gtttcacggt tatagcgttt gccatggcag acatcgcatg tcacgtaaat
     7741 atcgggaaga aaatgcatct caaccttgat catgccgtcg ccctggcagg cttcacagcg
     7801 tccgcccctg acgttgaacg aaaacctccc cggaccatag ccgcgctcac gcgcctgggg
     7861 cacgcctgca aatagctcgc ggattggggt gaacaggccc gtataggtgg ccgggtttga
     7921 gcgcggggtg cggccaatgg ggctttgatc catgttgatg actttgtcaa aaaagcccag
     7981 cccctcgatg gtctgatagg gcgcaggctc ggccgtgctg ccgtagaggt aatgagccat
     8041 tgcgttatag agggtctcat tgatgagagt ggatttaccg gaaccggaga cacctgtgac
     8101 gcaaacgaac aatcccagag gcaggttgag gtttacgttt ttcaggttgt ttcccgaggc
     8161 ccccgttatt ttcagccacc tgtccatttt tggttcggtg cgtttttcgg gcatttcaat
     8221 ggccagtgtt cccgaaagat attttccggt gagcgaatcg ggatcttcct gtatgtcggc
     8281 aggggtgccc tgcgcgatga tggccccccc atgctcaccc gcgcccggcc ccatgtccac
     8341 cacatgatcg gccgaaagta tcgcgtcctg gtcgtgttcc accacgatga cgctattgct
     8401 caaatcgcgc aggcttttca gcgtttccag caggcggccg ttgtcgcgct gatgcaaacc
     8461 gatggagggc tcatccagca catacatgac cccggtcaat cccgagccga tctggctggc
     8521 cagccggatg cgctgagact ctccgccgga caaggtttca gcggagcgtt ccagtgaaag
     8581 gtaatccagc ccgacgttgt tgaggaaaga gatgcggctg gcgatttcct tgatgatctt
     8641 ttctgcaatc gccaacttat gccccgtcag ttccacgttc tcaaaaaaag cctttgcttc
     8701 cttgagaggc agcgcgttga tctcataaat ggcgaggcca cccactcgga cattccgcgc
     8761 ttcgagacgc aggcgtgtac cgacacattc ggggcacacc tgcgagttga ggtacttgcc
     8821 aagttcttcg cgcacggtaa ccgattccgt c
//
LOCUS       DASSHJ010000139         9438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_773293, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000139 DASSHJ010000000
VERSION     DASSHJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773293"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VFI43_05465"
     CDS             complement(<1..1429)
                     /locus_tag="VFI43_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7061608.1"
                     /translation="MAYEATAIFAVVLLNAIMGYIQESRAEEAVAALRDMSAGRANVI
                     RGGNRHNIAVAEVVPGDIIIVEEGDTVPADARLIHTAALQTAEAPLTGESLPVSKDTA
                     AIPEEAGLGDRHNMIFSGTAVTFGRGRAVVVATGMQTQMGRIAGLLGEAPAEITPLQK
                     ELARVGKLLGIIVVCIAAIMIATIIVVEQMSSAAAIFDVLILGVALAVAAIPEGLPAV
                     VTAVLSLGVQRMARRNAVIRHLPAVETLGSATVIASDKTGTLTRNEMTVRAVVTASGR
                     VVLEGSGYSPEGEVRRTGPVKKNEIRKNEAREDEARKSEGEDDQEVISGPLRIELERA
                     LAVADRASNAVVHEQDGHWKVQGDPTEGALIVAARKAGPTDDALDARFKRVGEVPFSS
                     ERKLMSTLQTDVQKQEYVRVFTKGAPDLLLTRCTHELVGEETWPLPEERRTEILKVNE
                     ELAGEALRCIGVAYRSISSGAFSADE"
     gene            complement(1522..1713)
                     /locus_tag="VFI43_05470"
                     /pseudo
     CDS             complement(1522..1713)
                     /locus_tag="VFI43_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
     gene            complement(1970..2227)
                     /locus_tag="VFI43_05475"
     CDS             complement(1970..2227)
                     /locus_tag="VFI43_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7061609.1"
                     /translation="MQAEKKIAALESLKGRAFDKAYIDHKVAFHQGVLDTIDNRLMPN
                     AVNEDLKAFLYGLFPPLSEHLEHAQQIQESLNRGHRAKTVH"
     gene            complement(2583..2960)
                     /locus_tag="VFI43_05480"
     CDS             complement(2583..2960)
                     /locus_tag="VFI43_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061610.1"
                     /translation="MDKHPLIKLTWRGRETVESVPALFERAEQIEINLPPGYNHSLFC
                     LFHPHAPPSQLENIKISGGPQILERVASIKGLEEFKALTEPLTIAGVTVQVLSPPKIV
                     LTRPDAKVNKIKTKQVADDLTGQ"
     gene            complement(3171..3338)
                     /locus_tag="VFI43_05485"
     CDS             complement(3171..3338)
                     /locus_tag="VFI43_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061611.1"
                     /translation="MKQTLVMIAKTKPEQAMSARRVDRKADQKILGEGVAYRLAINGE
                     TQAVFLTPRPE"
     gene            complement(3340..4185)
                     /locus_tag="VFI43_05490"
     CDS             complement(3340..4185)
                     /locus_tag="VFI43_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061612.1"
                     /translation="MSIAWATVVIVVLLLPGFAFFWGLYAPNQVTREVSPASPLTQLA
                     GVVIISFFVHAFAYLVINRGLCVDFLSFPVPCVDFDELSALVRIDAFTLPGTAPATFR
                     DMLDFYIFWIVLYFLATGAFTGLLGFGAGKLIENGHLRGFTRHRYLFLLEKGRKSEES
                     ELVRAHVLSRTQNLNTIIFYDGILQDFFAKADGTISYIVLHDANTSVMEITASGPAHS
                     KNTIPLDVESGLRTTKPFLVITSENIANVYFEPLNTVRIESEADAEMLDDAIDRLEQS
                     EGEAE"
     gene            complement(4192..4398)
                     /locus_tag="VFI43_05495"
     CDS             complement(4192..4398)
                     /locus_tag="VFI43_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061613.1"
                     /translation="MRVLKEKDRILYDKLVRLAGGDSGIVIKVLSNPHRSSVDLKTAV
                     REIEELRNSRNRTPASLAPQAAAV"
     gene            complement(4624..5427)
                     /locus_tag="VFI43_05500"
     CDS             complement(4624..5427)
                     /locus_tag="VFI43_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061614.1"
                     /translation="MNLIQVKQVTLEYPIIGVGSRSVKNRVLSTATGGLITGGDAIPV
                     VKALQDVNFSLEEGDRLGLIGHNGAGKSTLLKVLAGIYKPTSGTVLVRGRIVSTLNLA
                     LGMEPEATGIENIFIRGLLLGMKKAEIRRKLDSIAEFTELGEYLHMPLRIYSSGMATR
                     LAFATVTAMEADILLMDEVIGTGDARFMDKTEHRLKDFIHRSKILVLASHTEAIIRNF
                     CNKVLLMEQGKIKGIGRVDEMFQLYYRHIHSARLLEYQGKRIDNTMAIG"
     gene            complement(5430..6398)
                     /locus_tag="VFI43_05505"
     CDS             complement(5430..6398)
                     /locus_tag="VFI43_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7061615.1"
                     /translation="MSESGVALQTWLHVHYALRLVPAVEALPALLGAATRPEISVNRG
                     RSSKHASRNSRMSFYGTLRKRFLESDLLEALKAYDVWLFLAWLDVRLRYRRSKIGPFW
                     ITLSMAIFCLTLGEVYSRLLNIEIAEYLPFLAISFVIWGLISSLLLEFPNLYVDNAAY
                     MKDMRVNALTILLRGIARNALVFAHNALIILGIYAYFDLWPGLIFLLVIPGLILVLLN
                     LFAIGVSLSIIGARFRDIAPINQSLVQILFFLSPIMWFPRLVSPDSLFLRLNPVAYFL
                     DLVRSPLLGSFPSVSSWYVAIAILILFTTVSSVLYMKKSSKIPLWV"
     gene            6692..8119
                     /locus_tag="VFI43_05510"
     CDS             6692..8119
                     /locus_tag="VFI43_05510"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET7061616.1"
                     /translation="MLLPDHIHFIGACGRAMSGVMAAVADLGVPVTGSDNENTYGSIR
                     RWLEKKGIIISSGFSSRNLNPVPALVVIGRQHGRGNTEVEYVLEKRIPYLSLPEFLCF
                     YFLNGSRNILVAGSKGKTTTTAMLVKILESAGLTPGYMIGGIPRSGIDPVRLGQSVYV
                     LEADDYSTLWWNDNPKALYYRPEVVVLCNSFSDHPEHHTNDDIRLRHFKALVEQLPQN
                     GLLVLADCRNSTDLDGLIGVARCQVRMVDMADGTGDAILGYKKVEDGVAFKWREAYFF
                     LEVNGYMNARNAVSAAMAAQHLGITPDIAASALFRFAGVSGRMDHALSTEELDVYIDC
                     FGYLPESLAENMAALREMHPRRRHVVVYQLLIVDGIPEAQRPLQISLAEWDEVLIVSY
                     ESPSPLLVSAGDDYLADLAATLRESGVDAAFIGSLENGLTVLPNLVKAGDVLFFSVHP
                     RSADLAWRIAQHTSTHENTHHEQYS"
     gene            8088..9278
                     /locus_tag="VFI43_05515"
     CDS             8088..9278
                     /locus_tag="VFI43_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7061617.1"
                     /translation="MKILITNNTLENLGGSEWVAIELAKSLASRGHEVAACSSQIGEA
                     GSLLRGMSVPTIPDPLDTPFKPDVIHGQHHLDTMRALCAFPDVPAIYHCHGYVPWVED
                     PPAHPRILYYVGMCSSISTRICLLLGLPDQKVTTVPNWVDLGRFRFVRNPAQKPQKAL
                     LYLRSFDRNGWHASQLRQAFETLGIKLDLWLPQGDTRAPEVALPEYDIVLASGRSAIE
                     AMASGCAVLPISPSSCLEFVDPGNFDAYQSQNFSPKLSAGHFNADSIKNVISSYDPGR
                     AAAVTTLVRSKCTLDAAIDILESLYVKTVKDFSEQCHLPSARYLSELNALTNYIQSVM
                     PMVRDRERLLAENHELQQVIAVKEQTIGKLLNCNSMRITRPIRAISSFLRRLRRGSRP
                     SYTC"
BASE COUNT         2163 a         2480 c         2502 g         2293 t
ORIGIN      
        1 cttcatcggc ggaaaatgcg ccggaagata tcgaacggta ggcgacgccg atacatcgca
       61 aggcttcacc tgccagttcc tcattcactt tcagtatttc cgtgcgtcgc tcttctggaa
      121 gaggccaggt ttcctcgccc accagctcat gtgtgcagcg ggtaagcagc aggtcaggcg
      181 cgccttttgt aaagacacgc acgtactcct gcttttggac atcggtttga agcgtgctca
      241 tgagcttacg ctctgatgaa aaggggactt cgccgactcg cttgaagcgc gcgtcgagtg
      301 cgtcatcggt cggtccagcc ttgcgggcgg cgacaataag cgcgccttcg gtgggatcgc
      361 cttgcacctt ccagtgcccg tcttgttcat gcaccacggc gttgctggca cggtccgcaa
      421 ccgctaacgc acgttccagc tcgatacgca acggcccgga aatgacttcc tgatcgtcct
      481 caccttcact tttcctggct tcatcctctc tggcttcatt cttcctgatc tcgtttttct
      541 ttaccggccc ggtccgccgg acttcgcctt cgggcgaata accgctgcct tccaggacca
      601 cccggccgct ggctgtgacg accgcccgca cggtcatttc gttccgggtc agcgttcccg
      661 tcttgtccga agcgatgacg gttgccgagc cgagagtttc gacagccgga aggtgacgga
      721 tgacggcatt gcgccgcgcc atgcgctgaa ccccgaggga gaggacggcg gtcaccacgg
      781 cggggagtcc ttcgggtatt gcggcaaccg cgagcgccac gccgagaatg agtacatcga
      841 aaatggccgc agcgctactc atttgctcga caacgatgat agtcgcaatc atgatcgccg
      901 cgatgcagac aacgattatc ccgagcaatt ttccgacgcg ggcgagttcc ttttgtagcg
      961 gcgttatctc cgccggagct tctccgagca gcccggcaat gcggcccatc tgcgtctgca
     1021 tcccggtcgc caccacgact gcgcgtcctc gtccaaaggt gacggcagtg ccactgaaga
     1081 tcatgttgtg ccgatcgccg agcccggctt cctcagggat cgcagccgta tcctttgaca
     1141 cggggaggct ttcccccgta agcggggcct cggccgtttg cagcgctgcc gtatggatca
     1201 ggcgcgcatc cgcggggacc gtatcgccct cttcaacgat aatgatgtct cccggtacca
     1261 cttccgccac cgcgatgtta tggcgatttc cgccacggat gacgttggcg cgccctgcgg
     1321 acatatctcg caaggccgcg accgcctctt ccgcccgcga ttcctggata tagcccatga
     1381 ttgcattgag cagcacgacc gcaaagatag cggttgcctc ataggccacg gcgtgtcctg
     1441 ctcgtatagc cagagtccca gcgagatcgc cgttgcgacc agcagcagga tgacaagcac
     1501 attctggaac tgcgcgagaa attttttcca ggcgggagtg ggtttttcgg ctgccagttc
     1561 attcctgcca tacctggata gccgtgcgcg tgcttcggct tcgctcaacc ccttacaagc
     1621 gtcagtgcca aatttaccca gcagctcatc caccggttcc cgataatggt cgtgagtaac
     1681 ctgctggttt tcggctgagg atgaaacttc catcggttgc tcatcgtttc attacaacaa
     1741 tcggactatc gaggtgaagg cttccttcgt ccctaaccga aaaacttcag gggccgcaag
     1801 agtggttcat agcttgctcg gcggtcgcct ttgacgggta tgtatctttg aagagtgagc
     1861 tctgcatatt tggttccggc ttgactgcag ccgcgtgcaa aagcgctgcg tgcgtgtatg
     1921 agggcacggg cagggcgatt tgcgcgagta cgggtagcgg gaggccgcct taatgcactg
     1981 tcttggcgcg atgcccgcga ttcagactct cctgaatctg ctgcgcatgc tcaaggtgct
     2041 ctgaaagcgg gggaaaaagg ccatacaaaa atgctttcag gtcttcattg acggcattgg
     2101 gcatcagcct gttgtcgatg gtatcgagga caccttggtg gaaagcaact ttatgatcaa
     2161 tgtaggcctt gtcgaaagcc cgccccttca atgattcgag cgcggcgatc ttcttctctg
     2221 cctgcaattc ccgctaatcg ggttgttcct gggtatcaga ttgagccgga tggcgagttc
     2281 gtttgtggat ttgtttacgc ccgaatgttc gtcgatcata cgacgcgcct atgccttgac
     2341 attgccatca gacgaatgcg tttgccaatt tgcccacttc aatcgcaatc tgattggcag
     2401 ctaccacgac gtccgctatc tgtgcgtcat ccatatcctt tatttccgca aatgctgcaa
     2461 gcggtgagcc attagtcccg gcccaaaaga aacaggcttt tcatgaagat ctccaagggg
     2521 agagcaagta gaaacagata gcctggcagc ccgtgaggga gccagaatat gcaccgcttc
     2581 tgctactggc ccgtcaaatc atcggcaact tgcttggtct ttattttatt gactttcgca
     2641 tccggccggg tgaggacaat ctttggcggg ctcaacacct gaacggtaac gcctgcgatg
     2701 gtgaggggct cggtcagggc tttgaattcc tccagtcctt tgatgcttgc cacgcgctcg
     2761 agtatttgcg ggccgcccga gatttttatg ttttcaagct gagatggggg cgcgtgggga
     2821 tgaaacaggc aaaacaggga atgattgtag ccggggggaa gattgatttc tatttgttcc
     2881 gcccgttcga atagcgccgg tacggattcg actgtctcgc ggccacgcca agtcaacttg
     2941 atcaacgggt gtttgtccat gatggcttcg tgatcgaatc aagaggttat atgtttcacg
     3001 aaaagccctt cggattccgt tcgctcgcgt acatactcgc tggaacggca tgacattaat
     3061 agtatgcatg acattaccgc caacgcgtat caggggggag cgttgcgcaa gccgaatttt
     3121 attgaatcgc agattccctt caaaaaatat ttcgatcagc gtagtttttc ctattctggc
     3181 ctcggcgtga gaaatacggc ttgagtctcg ccgtttatgg caaggcggta tgccacccct
     3241 tcacccagta tcttttgatc tgccttacga tcaacccggc gtgcagacat ggcttgttca
     3301 ggtttggttt ttgcgatcat tacaagcgtt tgtttcacgc tattcggctt ctccctccga
     3361 ttgttccagc ctgtcaatgg cgtcatccag catctccgcg tccgcctcgc tttctattct
     3421 taccgtatta agcggctcga aatagacgtt cgcgatgttc tcgctcgtga tgacaaggaa
     3481 aggttttgtg gtgcgcaggc cggattcgac gtcgagaggg atggtgttct tggagtgagc
     3541 cggaccgctt gcagtgattt ccatgacgga agtgttggcg tcatgcagga cgatataact
     3601 gatggttccg tctgccttgg caaaaaaatc ctgcaatatg ccatcataga atatgatggt
     3661 attgagattc tgggttcggg acaggacatg tgcgcgcacc agttcggatt cttcgctttt
     3721 ccggcccttc tccagcagaa acaggtatcg gtggcgggta aagccgcgaa gatgcccgtt
     3781 ttcgatcagt ttccccgccc cgaatcccag caggccggtg aaggccccgg tagccaggaa
     3841 gtaaagaaca atccagaaaa tataaaaatc gagcatatcc cggaatgtgg ccggggcggt
     3901 gccggggagg gtaaaagcat caatgcgcac caaggcggag agctcgtcga aatccacgca
     3961 gggcaccggg aacgatagaa agtccacgca gagtccccgg ttaatgacaa ggtaagcgaa
     4021 agcgtggacg aaaaagctga tgattacgac gccggccagt tgcgtgagcg gactggcagg
     4081 tgatacttcc cgggtaacct ggttcggggc gtagaggccc cagaagaaag cgaagcccgg
     4141 cagcagcagg acaacgatta caaccgtcgc ccaggcgatt gacatggggt gttacacggc
     4201 tgccgcttgg ggtgcgaggg aagcgggagt gcggttgcgg ctatttcgaa gttcttcaat
     4261 ttcccggact gcggttttga gatcaaccga agaccgatgg ggattggaaa gcacttttat
     4321 gacgatgcct gaatcaccgc cggcgaggcg aaccagcttg tcatagagga tcctgtcttt
     4381 ttccttgaga actcgcacgt tagtcccgat atttcgaaga acaatgatat atgaattttc
     4441 tagatatcat attagtcggc cggagtcaag ggactgcttg tctcaaagca cgatcaattt
     4501 atttttatca cggcgttcgc tgcggcgtgc gcttcactca caaatctcac gttcggcctc
     4561 gttcagcgcg gcttgtgttg ccccgatgca ctatcccgct ctggcgaatg ccgatggaag
     4621 acctcatccg attgccattg tattgtctat tcttttacct tgatattcca gaagccgggc
     4681 ggagtggata tgtcggtaat agagctggaa catctcgtcg acccggccta ttcctttgat
     4741 ctttccttgc tccatcaaga gtaccttatt gcaaaagttt cttatgattg cctccgtatg
     4801 cgaggcaagt accagtatct tgctgcgatg gataaagtcc tttaaacgat gctccgtctt
     4861 atccatgaaa cgcgcatcgc cggttccaat tacctcatcc atcagcagga tgtccgcctc
     4921 cattgcagta acagtagcga aggccaggcg agttgccatg ccggaagagt agattctaag
     4981 cggcatatga aggtactccc cgagctcggt gaactcggcg atactgtcca attttctgcg
     5041 tatctcagct ttcttcatcc caaggagcaa gccgcgaata aagatatttt cgataccggt
     5101 tgcctctggt tccataccca gggcgagatt cagcgtggaa acgatcctcc ctctgaccag
     5161 aactgttccc gaggttggct tgtagatgcc ggcgagaacc tttagcaagg tcgatttgcc
     5221 ggccccgtta tgtccgatca aaccgagccg gtccccttcc tcaagagaga aattcacatc
     5281 ttgcagcgct ttaaccacag ggattgcatc tccccccgtg atcaatccgc ctgtggctgt
     5341 actgagaaca cgatttttta cagaccggct ccctacaccg ataatcggat attcgagcgt
     5401 cacctgtttt acttgtatca ggttcatggt taaacccaga ggggaatctt gctgcttttc
     5461 ttcatgtaca aaaccgaact gacggtggtg aacagaataa gtatcgcaat tgctacgtac
     5521 caggacgaca cggatgggaa cgagccaaga agcggggatc gaacgaggtc gagaaagtat
     5581 gctaccggat ttaatcgcaa gaataagcta tcgggtgaga cgagacgcgg gaaccacatt
     5641 atgggagaaa gaaaaaaaag gatctggacc aggctctgat tgatgggagc gatgtcacga
     5701 aatcttgcgc ctattatgct caaggacacc cctatagcaa aaagattgag cagcacaagt
     5761 atcaagcccg ggatgacgag cagaaagata aggccaggcc aaagatcgaa ataggcgtaa
     5821 atccccaaga taataagagc attatgagca aacaccaaag cattccttgc tatcccccgc
     5881 agtagaattg ttagcgcatt gacccgcatg tccttcatgt aggcggcgtt atcgacatat
     5941 aaattaggaa actcgagcag gagactcgaa atcagccccc agataacgaa gctaatcgct
     6001 aaaaaaggca agtactccgc aatctctatg ttcaacagtc tgctgtacac ctctcccagg
     6061 gtcagacaga agatcgccat gctgagcgtg atccagaatg gcccgatttt ggagcgtcta
     6121 tagcgcaatc ttacgtcaag ccacgcgaga aacaaccaga cgtcataagc cttcagtgct
     6181 tcgagcaagt cgctctccag gaagcgtttc ctgagggtgc cataaaagct cattctggaa
     6241 ttcctgctgg catgtttgct gctcctcccc ctgttaacac ttatttccgg tcgcgtggct
     6301 gcaccgagaa gcgcaggcaa ggcctcaact gcgggcacca agcgcaaggc atagtgaacg
     6361 tgcagccaag tttgcaacgc aacgccactc tcgctcatcg cgaggatgcg cggaaatgtg
     6421 gcggttggtc acctgtaaaa tactacagct attccgcgaa cccgcccaca tgatgtaata
     6481 aaccgaaatc cgcaattttg tgaaatcact cccttcttaa gggagctgat tgcgcggcac
     6541 gaagaaactc cgcgatgcga gcgcactcgt tattgcggac cggaaatatt ttcattacca
     6601 cttttcgaag taaatggttg gctgagcgca acgagatcgc gtggcggcca gcatatccgg
     6661 ttctcatatt ctgaaaaatt atcgctaaac aatgctgctg ccggatcaca ttcactttat
     6721 cggagcttgc ggccgggcga tgtccggcgt gatggccgcg gttgccgacc tgggggtacc
     6781 ggttaccgga tcggacaatg agaataccta tggctcaata agacgctggt tagaaaaaaa
     6841 gggaataata atcagctcag gattcagttc acgtaatctt aaccccgtac ctgctcttgt
     6901 cgtaattggc cggcaacatg ggagaggcaa tactgaggtt gaatacgttc ttgaaaagcg
     6961 aattccctat ctctcactgc cagagtttct gtgcttttat tttctgaatg gcagtcgcaa
     7021 catcctggta gctggaagca agggcaagac cactacgaca gccatgctgg taaaaattct
     7081 ggaaagtgcc gggttgacgc cggggtacat gattggaggc atcccacgat ccggcattga
     7141 tcccgtcagg cttggccaga gcgtttatgt ccttgaagcg gatgattact cgacactttg
     7201 gtggaatgat aatccaaaag ccttgtacta ccggccagaa gtcgttgtgc tatgcaatag
     7261 cttcagcgat caccccgaac atcacaccaa tgacgacatc aggctgcgtc atttcaaagc
     7321 cttggtcgag caattaccgc aaaacggttt actcgtattg gcagactgca gaaactcgac
     7381 tgatctcgat ggtttgattg gagtggcacg ctgccaggtc agaatggtcg atatggcgga
     7441 tggaacaggt gacgctattc tgggatataa gaaagttgag gatggtgtcg catttaaatg
     7501 gcgcgaggcg tacttctttc tggaagtaaa tggctacatg aatgcccgaa acgctgtttc
     7561 tgcggcgatg gctgcgcaac acctgggtat cacccccgat attgcggctt cagctttatt
     7621 tcggttcgca ggtgtctctg gcagaatgga tcatgcgctc agcaccgagg aactggacgt
     7681 atatatcgat tgtttcggtt atcttcctga aagtcttgct gaaaacatgg ccgccctcag
     7741 ggaaatgcac ccccgccgtc ggcatgtcgt tgtttatcag ctacttatcg tcgacggaat
     7801 tcctgaagct caacgacctc ttcaaatcag tcttgctgaa tgggatgagg ttttgattgt
     7861 cagttacgag tcaccttctc ctctcctggt gtcagctgga gatgattatc tcgctgatct
     7921 tgcggcaacg cttcgggaaa gcggcgtaga cgcggcattc atcgggtcgc tggaaaacgg
     7981 tttgaccgtg ctacccaacc tggtaaaggc cggggatgtc cttttcttct cagtacatcc
     8041 caggtcagcg gatctggctt ggcgaattgc tcagcatacc agtactcatg aaaatactca
     8101 tcacgaacaa tactcttgaa aatctgggag gtagcgagtg ggttgcgatt gaactcgcga
     8161 aatctttagc ttctcgagga catgaagttg ctgcgtgcag ttcgcagata ggcgaagcgg
     8221 gatcattgct gagaggaatg tccgtgccca cgattcccga tccattggat acgcctttca
     8281 agcctgatgt cattcatgga cagcatcatc tggatacgat gcgggcttta tgcgcatttc
     8341 ctgatgtccc tgcaatctat cattgccatg ggtatgtgcc ctgggttgaa gaccctccag
     8401 cacatccgcg aatcctttac tacgtgggaa tgtgctcgtc aatttccacg cgcatctgct
     8461 tgttgttggg cttgccggat caaaaagtca cgactgtgcc aaattgggtt gatcttgggc
     8521 gatttcgttt tgtacgtaat ccggcgcaaa aaccccaaaa ggctttactt tatttaagaa
     8581 gctttgaccg caacggctgg catgcatctc agctccgcca ggctttcgag acgctgggca
     8641 taaagctgga cctctggctg ccccagggag acacccgggc gcccgaggtg gctctcccgg
     8701 agtacgacat agtacttgcc tccggccgat cggcaatcga ggcaatggcc tccggatgtg
     8761 ctgtcttgcc tatcagccct tcctcgtgtc ttgaatttgt tgatccgggc aatttcgatg
     8821 cataccaaag tcagaatttt tcgccaaaac taagcgcagg tcactttaat gctgacagta
     8881 taaagaatgt catttcctca tatgatcctg gtcgtgctgc ggccgtaacc actctggtca
     8941 gaagcaagtg tacgctggat gcagcaattg atattttgga atccttatat gtgaagacag
     9001 ttaaggattt cagtgaacag tgccatcttc cctcggctcg atacttaagt gaactgaacg
     9061 ctctcacaaa ctatattcaa tccgtcatgc cgatggtgcg ggatcgggag agattgcttg
     9121 cagaaaacca cgaacttcaa caggtaattg cagtgaagga gcaaacaata ggaaagctcc
     9181 ttaattgcaa ctcaatgcgc ataaccaggc caatcagggc gataagcagc tttctgagac
     9241 gtcttagaag aggctcgcgg ccgagctata catgctgatc cgtacgagga gcctcgaagc
     9301 ctaggtctgc ctgacgctat ttgcaggaat cctcctcacc ctcatgccgc tgttgcaagc
     9361 catgagcaag tggagcgctt ccttctaata tatcatgttg atgggttcgc tatgctccac
     9421 ccatcctgcc tgtaggtt
//
LOCUS       DASSHJ010000140        14360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1320382,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000140 DASSHJ010000000
VERSION     DASSHJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14360
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320382"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1037)
                     /locus_tag="VFI43_05520"
     CDS             complement(144..1037)
                     /locus_tag="VFI43_05520"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET7061618.1"
                     /translation="MIADFQDWAKSRQARIEDSLQALLPSPNVAPERLHEAMRYSVLG
                     GGKRVRPLLAFAAGELINAIEDRVVVVAAAVELIHAYSLVHDDLPCMDNDVLRRGKPT
                     CHVEYDEATALLVGDSLQSLAFQLMAEHCLADNPLIQLEMIKRLAHASGSRGMAGGQA
                     IDLGSVGKTLTLPELEFMHVHKTGALIRVSVMLGASCGGHLLEEQLGNLDHFAKCMGL
                     AFQVVDDVLDAEATTATLGKTAGKDADNNKPTYVSILGINRARELAEELRGEAYRSLE
                     SFGSAADRLRQVTDFIIQREF"
     gene            complement(1027..1281)
                     /locus_tag="VFI43_05525"
     CDS             complement(1027..1281)
                     /locus_tag="VFI43_05525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7061619.1"
                     /translation="MTKLAEPVKFSQPESFEAALAELESIVLEMEAGQLTLEMSLLVH
                     KRGTELLQYCQALLQDAQQQVRLLEADALKNFSLSGVDDC"
     gene            1504..2601
                     /locus_tag="VFI43_05530"
     CDS             1504..2601
                     /locus_tag="VFI43_05530"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7061620.1"
                     /translation="MTTLLDVSQFEQTQLPIEWYLDPGILDLERRLLFSRGPSYVGHE
                     LMVPNVGDYYVPEWMNGAKVLVHNENGLELLSNVCRHRQSLLLKGRGNIRNIVCPIHR
                     WTYDLKGTLLGAPHFPENPCLNLGSSSLQSWNGLLFEGKRNVAQDLRGLSVLKDFDFS
                     GHVLERVQIEQYECNWKTFIEVYLEDYHVDTYHPGLGNFVNTGELHWEFDEWYNVQIV
                     GINRGLTRPGTKVYARWHEQLLQQAGGNLPEYGAIWMLYYPNIMLEWYPHVLIVSTVL
                     PLTPDRCTNVVEFYYPEDIVLFERDFIEAEQAAYHETAAEDDEICRLMTAGRRALQQQ
                     GINEVGPYQSPMEDGMVHFHKFLRREMAANI"
     gene            2721..3065
                     /locus_tag="VFI43_05535"
     CDS             2721..3065
                     /locus_tag="VFI43_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HET7061621.1"
                     /translation="MVAIEEAGNVISVAVFGEFTLADYREFEEKVLHKSHADRTVNVL
                     FDWRDMVSYTVDVAWEDIKFIRKHGGEFNRVAIITEDQWRAWAAWVSNLFVDADIRVF
                     KDQAEAKAWVAT"
     gene            3691..5517
                     /locus_tag="VFI43_05540"
     CDS             3691..5517
                     /locus_tag="VFI43_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HET7061622.1"
                     /translation="MSSTIAKLNAPLTQAFDPQSSSELPAALIPTPTFNNQAQRLADF
                     DTLTAALEYAAEGTAGYNFYDAKGNLRSVLSYGALRAAAHSSAQRLSALRLVAGDRVA
                     IIADTTPEFIELFFACRYAGLIPFAMPVPVNLGSHAVYVQQLRGMLEGGQASAALANA
                     DYITFLHEAAEGLQGLRWVGTPEQLGQLAGLDIAPRENNDDEIAYLQFTSGSTRIPRG
                     VIITEQALMSNLRGIVRSGLDIHPGDRCASWLPFYHDMGLVGMVMAPMVAQVSVDYLA
                     TRDFAIRPLQWLRLISRNRATVAFSQPFGLKLCTLRVRDSDLEDLDLSSWRAAGVGAE
                     MIRPDTLRNFAEKFAPAGFDARAFLPCYGLAESTLAVTFSQIGNGFQSLRVDAGTLVD
                     RKLAVRLQVEGRKYSEFVNCGRPLPGHTVKVIDDAGQELGDLKVGSVLVHGSSVMTGY
                     FNNPGDTQKTLQPGGWLDTGDLGFVFEGDLYVTGRRTDVIIVNGRNIRAQDIEELAEQ
                     QPEVRTREASAFGITDASDVTTIVLVIECRLTSTTDRQSLTSRLQRLVYMAFGVNCLV
                     ELVPPHTLPRTSSGKLSRFAARQGFLQRTKLTDRLSAESGEI"
     gene            5526..6437
                     /locus_tag="VFI43_05545"
     CDS             5526..6437
                     /locus_tag="VFI43_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7061623.1"
                     /translation="MPEPVVAVTGATGFIGHVLLKALIKEGWKVRALSRRPRSSDEAV
                     EWVIGDLDNHGALRKLVKQVSAVIHCAGQVRGDSLEAFTRTNVEGTANLLNALDQDSP
                     PRFLLISSLAAREPELSWYAASKRMAEQLVLARSTILPVAIFRPTAVYGPGDKEMSPL
                     FRLTRRGILPMVGTPGMRFGLLHVTDLVRAVLCWLSTNPVVQGIYELDDGTLGGYDSR
                     TVASIAQEVWRRPVHCLFLPAHLVSMIANMNLWAARLLGYAPMLTPGKVRELQHSNWV
                     CDINPLTQALPQWQPAIRLRDALHQAV"
     gene            6415..6720
                     /locus_tag="VFI43_05550"
     CDS             6415..6720
                     /locus_tag="VFI43_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7061624.1"
                     /translation="MPCIRQFDQTYFQFTPDMLADYDQVLQRLCHHLKKFCPPEIEIT
                     PQTDLIDQLAIDSVKLLNLIMEIEDEFDISVPLNALADVQTVDELANLICKIRSTTS"
     gene            6717..7901
                     /locus_tag="VFI43_05555"
     CDS             6717..7901
                     /locus_tag="VFI43_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7061625.1"
                     /translation="MKLLDKLDIVAATRKALLPEGIDVFGIPIEEIYSSTEARINDRR
                     ILFLGTNNYLGLTFSPECLQAAHDAIASEGTGTTGSRMANGSYSGHRALESEFAEFYG
                     CQSCIVFTTGYQANLATISGLAGTGDIVLIDGDSHASIYDGCRLSGAEIIRFRHNDPK
                     DLEKRLKRLGERSTSTLIVVEGIYSMLGDKAPLADIVGLKNAYSSTLLLDEAHSLGVL
                     GQTGQGLVEETGLINEVDFITGTFSKSLGGIGGFCVSNHPQLDQLRYISRPYIFTASP
                     TPATIASTRAALRLLKDGVRLRGQLWQNARQLYSRLSELGYQLGPEASPVIATIVDTP
                     QQAIALWKGLLDRGIYVNLILPPAAPEGKSLVRCSVSAVHNDEQMRYVGDMFAELREL
                     VQ"
     gene            8377..9318
                     /locus_tag="VFI43_05560"
     CDS             8377..9318
                     /locus_tag="VFI43_05560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7061626.1"
                     /translation="MKRFSPAISIIIPTYNCESYIAETINSVLNQTFSDFELIVIDDG
                     STDHTRQIASSYGENVRLITQANAGVCKARNRGIREAAGRYLCLMDHDDYWFPDKLAL
                     QMDTMERHPEAGLVYSTFNWWFPDANGAFPPVEAYIEDPCPEGIDEDFSGWIYHLLLL
                     DCWVLTSSALIRAEVFGRCGMFDESLPYSEDWDLWLRIAREYPFIKLKKSLTLYRQHA
                     YQGNLVTRVIDYRTDLLTTAAEKWGLCSPDGRCITKQQFRRTISRYHRDFGIRHLLSG
                     NLRIANRSFIKSWLTSPVSLKSLAYIPAGLLGWRPKW"
     gene            9395..10363
                     /locus_tag="VFI43_05565"
     CDS             9395..10363
                     /locus_tag="VFI43_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7061627.1"
                     /translation="MASFDVFNGDADGICALHQLRLAEPVESALVTGVKRDIELLKNV
                     EASAGDHVTVLDISLDKNREPLRSLLNMGATITYIDHHFPGDVPDHPALKTYIDRSPG
                     TCTSSLVNDILQGRFRIWAVVAAFGDNMGQCAVDLAAPLRLTGDQLAVLRELGEYLNY
                     NAYGDDVDDLLFHPADLYRRLHRYCDPFEFIETEAVFESLKNAFQADMFMAGGLHPTY
                     SYPGGAVYILPDQPWSRRVSGTFGNHLARAEPGLAHALLIPNRDGGYLASVRAPRARP
                     TGADELCRLFETGGGRKSAAGINSLPENDLPRFLGSFRDAFPTLLA"
     gene            complement(10400..11377)
                     /locus_tag="VFI43_05570"
     CDS             complement(10400..11377)
                     /locus_tag="VFI43_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061628.1"
                     /translation="MRPSSALIILGMHRSGTSLLAGLLSQVGVVMGKRLYAPQKGVNE
                     KGFWEHEDIVDTHDELLLMLNSQWDDVLPWPEKWWESEAVQPFIARLLAYVRRDFSNA
                     DVWALKDPRMCRLLPLWLPILTREPIRPVFICMNRNPFEVVGSLQKRDGFSREKGLVL
                     WLNHALSAERYSRGYSRVFTDFDQVVKKPSEVLLKIEKEADIVFPKPVDTAAGEIGRF
                     VSPDLRHHKADTPPNDGHEELALMADKLYRHLVKMSGGDETQYELIDKIAADFLAYQN
                     KWNPELIEQIHYLNQERADYRVKFFRIYKSWSWQLAKPVWLIEKMLRKY"
     gene            11938..12639
                     /gene="cysC"
                     /locus_tag="VFI43_05575"
     CDS             11938..12639
                     /gene="cysC"
                     /locus_tag="VFI43_05575"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET7061629.1"
                     /translation="MQRPPRASDAGNPPITMIAKTDLVWHNATITRYHREHLNLHRSA
                     VLWFTGLSGSGKSTLAHAVEDRLYQLNCRTFVLDGDNVRQGLCSDLGFSAGERSENIR
                     RISEAAKLLVEAGVIAMTAFISPFRSDRQRARNIFPHGDFIEIYCDASIDICEQRDVK
                     GLYKRARKGEVKEFTGISSPYEAPVNPELIIRTGELTLESCVMQVIKYLGQRNIFNNN
                     PNALIGTKTTSPGAA"
     gene            12636..13985
                     /locus_tag="VFI43_05580"
     CDS             12636..13985
                     /locus_tag="VFI43_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HET7061630.1"
                     /translation="MRDILVTLVVFGLLPFVFKKPYVGALMWVWISVMNPHTQGWGFA
                     GTFPFAAIIAGAAIISLMLTKEPKSLPLTPVTVVFIVFVLWMNVTTVLGFYPEESFEQ
                     WNKVMKIMFMTLVVLMLVKTEEHIKRLIWVIVISLGYYGVKGGIFTIVSGGVDMVLGP
                     QGTFIGGNNEIALALILTIPLMHYLQTITSSIWIRHALTVAMLLSALAALGSYSRGAL
                     LAIAMMGFFLWLKSRHKLRIGLLLLLAVPPAIGFMPEKWAARMDTINTYEEDGSVLGR
                     FNAWWMAYNLAKDRPLTGGGFEIITPDLFYLYAPNPEDLHAAHSIYFQALGEHGFVGL
                     GLYLLLGFLVWRTGSWIIRNTSGREEYRWASSLATMVQVSLVGFTVGGAFLSLLYFDV
                     PYYLMCAMVATRILVAKRLKEAPASIGRDQIADSKQTPIPAQVGSFGPSRSHSAVRD"
     gene            14043..>14360
                     /locus_tag="VFI43_05585"
     CDS             14043..>14360
                     /locus_tag="VFI43_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7061631.1"
                     /translation="MPEHQSIDLEAQPRPVHSAAFLPSQLLLTLLSPGGQRGRLSTLI
                     FHRVLPRPDALFPEEGDAESFEAQIAHLAACFNIIPLPEAIQALRRGKLPKRAACITF
                     DDGY"
BASE COUNT         3344 a         3646 c         3714 g         3656 t
ORIGIN      
        1 ctcgaccagg aactgcctta actcatcggc aagctgaaga agtgccttgc gctccagcag
       61 acgcaatttg gaagggctgt taatggtgtc aagcaatgaa tacatttagg ccttcaatag
      121 aagcttcacg gataaggata agcttaaaac tcgcgctgga tgatgaagtc ggttacctgc
      181 cggagccggt cggcggccga tccgaaactc tcgagcgagc ggtatgcctc acctcttagt
      241 tcttcggcaa gctcacgcgc ccggttgatt cccaggatgc ttacataagt cggcttgtta
      301 ttatccgcat ccttgccagc agttttcccc agcgtagctg ttgtggcttc cgcatcgagc
      361 acatcatcga caacctggaa tgcaagcccc atacattttg cgaaatgatc cagatttccg
      421 agttgttcct cgagcaagtg tcccccacaa gaggcgccaa gcattaccga cacacggata
      481 agcgcacctg tcttgtgaac atgcatgaat tccagttcgg gaagagtgag cgttttgcca
      541 acgctcccca agtcgattgc ctgtccacct gccatgcccc tggagccgga agcatgggcg
      601 agccgcttta tcatttccag ttgaatcaga ggattatcag ccaggcaatg ttcggccatt
      661 agctgaaaag caaggctttg caggctgtct cccaccagca gggccgttgc ttcgtcgtat
      721 tcgacgtggc aggtaggttt ccctcgtcgc agcacgtcgt tatccatgca gggaagatcg
      781 tcgtggacaa gggaataggc atgtatcagt tccaccgcag cggcaactac gactacccgg
      841 tcttctatcg cattgatcag ctctccggct gcaaaggcga gcagcggccg gactcgcttt
      901 cccccgccga gaacggaata acgcatggcc tcgtgcaggc gctcgggggc gacgtttggg
      961 gagggaagca gggcttgaag ggagtcttct atgcgtgcct ggcggctttt tgcccagtcc
     1021 tggaaatcag caatcatcta caccggacag ggaaaaattt ttcagtgcgt cggcttccag
     1081 cagacgtact tgttgctgtg catcctgaag caaagcctga cagtactgca acaattctgt
     1141 gccccgctta tgaactaata acgacatttc gagtgtcaat tggcctgctt ccatttccag
     1201 cacaatactt tccaattcag ccagtgctgc ctcaaaactt tcaggttgtg aaaatttgac
     1261 gggttcagca agctttgtca tgataagcga ggtatgtaga acctccgatg ggaaactgca
     1321 aaaataacgc aataagcggg ttcaggtcaa ttaaatccac gaaactttcc acaagagaac
     1381 gtctcttagt cgcgattata agcgtttcga gaaagtagta tccgctctct catcttgcca
     1441 ctgtcttttt tcaagactcg atcaactgtt tattctggcc agaaaagtta agtacattaa
     1501 tctatgacta ctctccttga tgtttcccag ttcgagcaaa cgcagttacc catcgagtgg
     1561 tatctcgacc cgggcattct cgatctcgaa aggcgcttgc tcttcagccg cgggcccagc
     1621 tacgttggac atgagctgat ggtcccgaat gtaggtgatt attacgttcc cgagtggatg
     1681 aatggagcca aggtactggt gcacaacgag aacggcctcg aactgctgtc caatgtttgc
     1741 cgtcatcgcc aatcgttatt gctgaaaggc cggggaaaca tacgcaacat tgtttgtccc
     1801 attcatcgct ggacctacga cttgaaaggc actctgctcg gcgcaccgca ttttccggaa
     1861 aatccttgcc tgaatctggg aagctcttcg cttcagagct ggaatggcct gctgtttgag
     1921 ggcaaacgaa atgtcgcgca agatcttcgg ggcttgagcg tactcaagga ttttgacttt
     1981 tccggtcacg tactggaacg cgtgcaaata gaacaatacg aatgcaactg gaaaacattt
     2041 atcgaggtgt atctggagga ttaccacgtt gacacttatc atcccggatt gggcaatttc
     2101 gtcaatactg gcgagttgca ttgggaattc gacgaatggt ataacgtaca gatcgtcggg
     2161 ataaatagag ggctgacgcg gcccgggacg aaagtgtacg cgagatggca cgagcaacta
     2221 ttacagcagg ccgggggcaa tcttcccgag tacggtgcca tctggatgct ctattatcct
     2281 aatatcatgc tggaatggta tcctcatgta ttgattgtca gcaccgtctt gcctttaact
     2341 cccgatcgct gcactaacgt agtggagttt tattaccccg aggacatagt attgttcgag
     2401 cgcgacttca tcgaagcaga gcaagccgct taccacgaga cggctgcgga agacgatgag
     2461 atttgcaggc tgatgactgc tggacgtagg gctctgcaac agcagggaat taatgaggtt
     2521 ggtccttatc aatcgccaat ggaagatgga atggtccatt ttcataaatt tctccgccgg
     2581 gagatggccg ctaatattta gttgagtcag caggagaacg agtcgctaac tctgcctcga
     2641 tcggaaaggg gggcgcctcg atccagaata accgatcgtg aaaattcgat aacgaatgga
     2701 gaactctcga gttccaggac atggttgcca ttgaggaagc agggaatgta atcagcgttg
     2761 ccgttttcgg agagtttacc ctcgctgact atagggaatt cgaggagaag gtgctccata
     2821 aatcccatgc cgacaggacc gtaaacgtct tgtttgactg gcgcgacatg gtgagctaca
     2881 cggtggatgt cgcgtgggag gatatcaagt tcatacggaa gcacggcgga gagtttaacc
     2941 gtgtggcaat aattactgaa gatcagtggc gggcctgggc agcctgggta tccaatctct
     3001 tcgtcgatgc cgatatcagg gttttcaagg atcaggccga agcaaaagca tgggtagcaa
     3061 cctgatccgt aatatcaatg catttgcctt cagttgcttc gatatcagca tttcaaattg
     3121 cttgaatccg ggcaggaagg tttgaccggt cctccggaat tccttttcgc cgccccggat
     3181 ttgtttgcat attcgcgttt ccgagttttt gagaaactca gcgctccctt tgtaaacccc
     3241 gagggtaagt ggtgctgagg caaagcacgg ctatatgttg caaaaaagcc acacattccc
     3301 cttatatttt ccaaaatttc tttatattgg cggataactc gtacagaatg gaacctgtcg
     3361 ctaccgcaag tattaggcgt gacccccccg gttatcttta tggcatattg catctgcgaa
     3421 aatgggctcc aatcctgggt tttgatatga ttcgggctga ttccgatctt gccttgacag
     3481 taaaaactac tataatataa gctcattaat taaccgaatt agcgtgatta cttgccggta
     3541 attgcaaggc tgcagccaag gtatttgttt aagattccgc ttcaggaaga ccagcggtga
     3601 acaaatggcc tacaggataa atgtgtgtcc gattggaacc ctttacacta taaaaagatg
     3661 acaagtacca tcaatttgga ggggaaaacc ttgagttcaa cgatagcgaa gttgaacgct
     3721 ccgctaacgc aagcgtttga tcctcaatcg tcaagcgagt tgccggcagc actgattcct
     3781 acccccacgt ttaacaacca ggctcagagg ttggcagact ttgatacttt gaccgctgct
     3841 ctggagtatg cagcggaggg tacggcggga tacaattttt atgatgccaa aggtaatctg
     3901 cgttcggttc tttcctatgg agccctgaga gccgctgctc attcgtcagc ccaacgtctg
     3961 tcggctttga ggttggtcgc tggcgatcgt gtagcgatta tcgcggatac gacccctgaa
     4021 ttcatagaat tgttttttgc gtgccgttat gcggggctga taccttttgc aatgccggtc
     4081 cccgtgaatc ttggcagtca tgcggtatac gttcagcaac tccgcggaat gctggagggc
     4141 gggcaggcaa gtgctgctct tgccaatgcc gactacatta cttttcttca cgaagcggcc
     4201 gagggcttgc aagggttacg ctgggtcgga acgcccgagc agttagggca attggcaggc
     4261 ctggatatag ccccacgtga aaacaatgat gacgaaattg catacctgca gttcacgtcc
     4321 ggcagcaccc ggattccgcg aggggtcatt attaccgaac aggcattaat gagcaatcta
     4381 aggggaattg ttcggagtgg attggacatc cacccgggtg atcgctgtgc ttcctggctc
     4441 cctttctacc atgatatggg gttggtgggc atggtaatgg cccccatggt agcgcaggtt
     4501 tcggtcgact acctggcgac gcgcgatttt gcaatcaggc cgttgcaatg gttgcgcctg
     4561 atcagcagaa atcgtgctac ggtcgcattc agccaacctt ttgggctgaa gctttgcact
     4621 ttacgggttc gtgattctga tctcgaggat cttgatctga gtagctggcg ggcagcgggt
     4681 gttggcgccg agatgatacg gccggatact ttaaggaatt ttgcagaaaa gtttgctcct
     4741 gccggtttcg atgcgagagc atttcttcct tgctacggtc ttgccgaatc aacccttgct
     4801 gtaaccttct cccagattgg caacggtttc caaagcctca gagttgacgc agggacactt
     4861 gttgaccgca aattggcagt caggctgcag gtagagggcc ggaaatacag cgagtttgta
     4921 aattgcggcc gccccctgcc cgggcatacc gtcaaggtaa tcgatgacgc agggcaggag
     4981 ttgggtgatc tgaaggttgg gagcgtgttg gtgcatggca gcagtgtcat gaccggttac
     5041 tttaacaatc cgggtgatac tcaaaaaacg cttcagcctg gcggatggct tgatacaggt
     5101 gatcttggct ttgttttcga aggcgatctc tacgttactg ggcgccggac agacgtcatt
     5161 attgtcaacg gtcgtaatat acgcgctcaa gatattgagg aattggctga gcaacaacct
     5221 gaggtaagaa cgagggaggc atctgccttt ggcataacgg acgcaagcga tgtgacaaca
     5281 atcgtccttg tgatcgagtg tcgcctgact tcgacaacag atcgtcaatc gctgaccagt
     5341 cgattacaga ggctcgttta tatggctttt ggcgttaatt gcctcgtaga actggtcccc
     5401 ccgcataccc tgccgcgaac ttcgtcaggg aagctttcac gttttgcggc tcgccaaggc
     5461 ttcctgcaac gaaccaaact gacagaccgg ctatcagctg agtccggaga aatataaacg
     5521 gaaatatgcc ggaaccagtg gtggcggtaa ccggcgctac cggttttatc ggccacgtac
     5581 tgttaaaggc tctgatcaag gaagggtgga aagtcagagc cttgtccaga cgcccacggt
     5641 cgtccgatga ggctgtcgaa tgggtgatag gcgatttgga caaccacggc gcattgcgga
     5701 aacttgtcaa acaggtttcc gctgttattc attgtgccgg acaggttcgc ggcgactctc
     5761 tggaagcctt tacccgtacg aatgtagaag gtaccgctaa tctattaaat gctttagatc
     5821 aggattcccc tccccgtttt ttactcatat cgtcactcgc cgcgagagaa cccgagcttt
     5881 cctggtacgc agcgagtaag cgcatggctg agcagttagt actggctcgt tctactatct
     5941 tgccagtcgc aatatttcgt cctaccgcgg tttacggtcc gggcgataag gagatgagcc
     6001 ccctcttcag actcacccgg cgcggtatac tgcctatggt gggtacgccc ggaatgcgct
     6061 ttggcttgtt gcacgttact gatctcgtgc gggccgttct ttgctggctt tcgaccaacc
     6121 cggttgtgca aggtatttac gagcttgatg atggaacgct gggagggtac gacagccgta
     6181 cggttgcttc aattgcgcag gaggtctggc ggcgccccgt tcattgtctt tttttacccg
     6241 cccaccttgt gtcaatgatc gctaacatga atttgtgggc agcacgattg ctaggctatg
     6301 ctccgatgct gacaccaggg aaagtcaggg aattgcagca ctccaattgg gtgtgcgata
     6361 taaacccgtt gacacaagct cttcctcaat ggcaaccagc catcaggttg cgtgatgcct
     6421 tgcatcaggc agtttgatca aacctatttt cagttcaccc ccgatatgct cgcagattac
     6481 gaccaggttt tgcaaaggct ctgccatcac ctgaaaaaat tctgtcctcc tgaaatagaa
     6541 attacacctc aaactgattt gatcgatcaa ctggctattg attctgtaaa attgctgaac
     6601 ctgatcatgg aaattgagga tgagtttgat atttctgttc ccctcaatgc gctcgcggat
     6661 gttcagaccg ttgatgaact cgcgaatctt atttgtaaga taaggtccac tacatcatga
     6721 agctgctgga taaactggac attgttgctg ctacgcggaa agctcttctg cccgaaggga
     6781 ttgatgtgtt tggaatacct attgaagaaa tttattcttc gacagaggca agaatcaacg
     6841 atcgtcgcat cctgtttctg ggaacaaata attaccttgg tctgactttt tcgcctgaat
     6901 gcctgcaagc agcacatgat gcaattgcca gcgagggtac gggaacgacg ggttcgcgga
     6961 tggctaacgg aagctatagc ggacataggg cgcttgagtc agagtttgca gagttctacg
     7021 gttgccagtc atgcatcgtt tttaccactg gctaccaggc gaacctagct accatttccg
     7081 gacttgccgg gacaggcgat atcgtcctca ttgacggcga ctctcatgcg agtatttatg
     7141 atggctgccg cttgagcgga gcggagatca tccgtttccg ccataatgat ccgaaggatc
     7201 tggagaagcg gttgaagcga ctgggcgagc gttcaacctc gacgcttatc gttgtggaag
     7261 gcatctatag catgcttggc gataaagcgc cgctagcgga tatcgtcggg ttgaaaaatg
     7321 cttacagcag tacgctcctg ctggacgaag cccattccct cggtgtttta ggtcaaacgg
     7381 gtcagggcct tgtggaggaa acgggactta tcaacgaagt cgattttatc actggcacgt
     7441 tcagcaagag cctgggaggt attggcggtt tttgcgtgag caaccatccg caactcgacc
     7501 agctgcgcta tatcagccgc ccgtatatat tcactgcttc gcccacgccc gcgacgatcg
     7561 cttcaacacg agcagcgctg aggctgttga aggacggtgt ccgcttgcga ggtcagcttt
     7621 ggcagaacgc aaggcagctt tactcgcggc tttccgaatt ggggtatcaa cttgggccgg
     7681 aggcgagccc tgtcattgcc accatagtcg atacccctca gcaagctatt gcgctatgga
     7741 agggtcttct tgatcgaggc atctatgtca acctgatcct gcctcccgct gccccggagg
     7801 gaaagtcatt ggtacgctgc agcgtgagcg ccgtgcataa tgatgagcaa atgagatacg
     7861 tcggggacat gtttgcagag cttcgtgagc tcgttcaata agcttcgctc gatcccctca
     7921 ttaaaaacgt cattcttgac cggatgttgt tgtcctgcaa accgaactca tcggcccgct
     7981 gagtaatagg aagcttccgc tatggttgac gattaccccc gcgtgcgtct tctcccgcag
     8041 ccatctcctt tggcttgtta ggaggtttgc tcctatttga agatatgatc ggataatagc
     8101 gaggtattcg ccctatcaat gagcacacaa tgcgcgatca tgtcacattc gcttccgcca
     8161 taaatgcgag attgttgtaa cagagcagca ggatatgccc gctccggagt tcgggtaaag
     8221 caaacatgaa tatctaaagt ggcagaaagc catatctcta cctttatccg ccatggaatg
     8281 gagcaggatc ctgattagcg ccagtagcct tcatccacga agggttgatc cgtatttcgt
     8341 catatttgat cttatccagc cagatcagga tccagcatga aacgcttcag ccccgcgatc
     8401 tctatcatca ttccaaccta taactgtgaa agctatatcg cggaaacgat caatagcgtg
     8461 ctcaaccaga ctttcagcga ttttgagctc attgtcattg atgacggatc caccgaccat
     8521 acccgccaaa tcgcctcttc atatggggaa aacgtccgct tgatcacgca agccaacgca
     8581 ggtgtctgca aggcacgtaa ccgcggaatc cgtgaagctg ccggacgcta cctttgcctc
     8641 atggatcacg atgattactg gtttccggac aagctcgcgc ttcagatgga caccatggag
     8701 cgccatcccg aagcaggtct cgtttacagt accttcaact ggtggtttcc cgatgcgaat
     8761 ggcgcttttc cgcccgttga agcttacata gaagatccat gtccagaggg catcgatgag
     8821 gatttttcag gctggatata tcacctgctt ctacttgatt gctgggtact gacgagttca
     8881 gcgctcattc gcgcggaggt tttcgggcga tgcggtatgt ttgatgaatc cttaccatac
     8941 agcgaggatt gggacctgtg gctgcgtatc gctcgggaat atccgtttat caagttaaaa
     9001 aaatcgctga cgctctatcg ccagcatgcg tatcagggaa acttggtaac aagagttata
     9061 gactaccgga ccgatttact gactacggcg gcggagaaat ggggactatg cagtcctgat
     9121 ggccgttgta ttactaaaca gcaattccgg cggaccattt cgcgctacca ccgcgatttt
     9181 ggcatccggc atcttttatc gggaaatctg cggatcgcca atcgatcatt tataaaatca
     9241 tggttaacct cccctgtcag tcttaaaagt ctcgcgtata ttcccgccgg actactgggt
     9301 tggaggccca aatggtgagg ctggctcatc aggctttgct gaacgccctg gggtcggcat
     9361 gcaccctggc ttctgaggca gaacggtatt tttgatggct agcttcgacg tatttaatgg
     9421 agatgccgat ggtatttgcg ccctccatca actgagactg gcggaaccgg tcgagagcgc
     9481 cctggtcacg ggtgtcaagc gagatataga actattgaaa aacgttgaag cttcggccgg
     9541 tgatcatgtc actgttctgg atatttctct cgacaaaaac cgggagcccc tgcggtctct
     9601 tttaaacatg ggagcgacga taacttacat agatcatcac tttccaggag atgtgccaga
     9661 ccatccggca cttaaaacgt atatcgatcg ttcacccgga acatgcacga gttcgctcgt
     9721 caatgacatc cttcaaggca ggtttcgaat atgggcagtt gtcgcagcct ttggggacaa
     9781 tatgggtcaa tgcgccgtgg acttggcggc gccattgcgt ttaaccggcg accaactggc
     9841 cgtattgcgc gaactcgggg aatatctcaa ttacaatgcg tatggtgatg atgtcgacga
     9901 ccttcttttt caccccgccg acctttaccg acggttgcat cgttactgtg atccctttga
     9961 atttattgaa acggaggccg ttttcgagag tctcaagaac gcttttcagg cggatatgtt
    10021 catggctgga gggctgcacc cgacctatag ctacccgggt ggcgcagttt atattctgcc
    10081 tgaccagccc tggagccgca gggtgagcgg gacgttcggc aaccatctgg cgcgcgcgga
    10141 acccggcctc gctcatgctc ttttgatccc gaacagggac ggaggctatc tggcgagcgt
    10201 tcgcgcgccc cgcgcccgac ccacaggggc ggatgaatta tgccgcttat tcgagactgg
    10261 cggcggacgt aaaagcgccg caggcatcaa tagtcttccc gaaaatgatc tgccgcgttt
    10321 ccttgggagc tttcgcgacg ccttcccgac tctgctcgca tagagcggcc cgtctcccca
    10381 agttttctgg agctcagtct cagtatttgc gcaacatctt ttctatcaac cagactggct
    10441 tggccaattg ccaactccag gatttataga ttctaaaaaa tttgaccctg tagtctgctc
    10501 gctcctggtt caggtagtgg atctgctcga tcagttcggg attccactta ttctggtacg
    10561 ctaaaaagtc cgccgcaatc ttgtcaatca attcatattg cgtctcatct ccgcccgaca
    10621 tttttacaag gtgtctataa agtttgtcag ccattaatgc gagctcttca tgaccgtcgt
    10681 tcggcggggt atcggcttta tggtgacgga gatcgggcga aacaaagcgg cctatttcgc
    10741 ccgctgctgt atcgacaggc ttgggaaaaa ctatatctgc ctctttttct atctttaaaa
    10801 gaacttctga aggctttttg acgacctggt caaagtcagt gaaaactctt gaatatcccc
    10861 tggaatagcg ttcggctgac agcgcatgat tcaaccataa tacaagcccc ttttctctgg
    10921 aaaaaccatc ccgtttttgt agcgaaccta cgacctcgaa tggattcctg ttcatacaaa
    10981 taaaaacagg acgaatcggc tcacgagtca gaataggaag ccatagcggt aacagacggc
    11041 acatccgggg atctttaagc gcccaaacgt cagcattcga gaaatccctt ctgacataag
    11101 cgagcagacg ggcgatgaat ggttgaaccg cctcgctctc ccaccacttc tcgggccagg
    11161 gcagcacatc gtcccattgc gaattcagca tgagcagtag ctcatcatgc gtgtcgacaa
    11221 tgtcctcatg ctcccagaag cctttttcgt taacgccttt ctgtggagca tacaagcgct
    11281 tgcccattac gacaccgact tggctcagta atcccgcgag cagcgacgtt ccgctccgat
    11341 gcattcccaa aatgatcagc gcactgcttg gtctcacttg aaatcctctt tcagggctta
    11401 cctcgaaggc tttttatccc ggttttcagc cccttgattg ctggcgtaca gaatatacgc
    11461 taatgccaga tcagttagcc ttggataacg gacgggaaaa gccgccaatt acccatacct
    11521 acttccttca tcacgttcat aatcgctgaa gttgcggccc atgtatagga actctttggt
    11581 ttcccactcg atacgattac aaaacctcta tcatgttcga aaagcgcaac cccgcgtcgg
    11641 ataaatcttc accgttgggc ttccgtcaca tggtctgacg ttcgttatcc gcgatttatc
    11701 caccctgcag tgctgtgaat ggcacgcatt tatggcttga acgattgcaa gcatgttgag
    11761 gcggtaacaa tggtccccga tcccgatgct tttctctgat gaattttgtc actccgtccg
    11821 gtatcatcct catcgcggta ctcaaacctc aaacctcaaa tctgcaaaac ctctcattat
    11881 tttatccagc ctgcggcctg ttaaaaaaca gaagtacgct attccacatt aaaggatatg
    11941 cagcgaccgc cgcgggcgtc ggacgccggg aatccgccca tcaccatgat tgcaaaaact
    12001 gatcttgtct ggcataacgc caccattacc cgttatcacc gggaacactt gaacttgcat
    12061 cgttctgcag tgctatggtt caccggactt tccggttcag gtaaatctac cttggcccat
    12121 gctgtagaag atagactata ccagttgaac tgtcgcacct ttgtgctgga tggggacaat
    12181 gtacggcaag gtttgtgttc tgatctgggt tttagtgctg gggaaaggtc agaaaacatt
    12241 cggcgtataa gcgaagcggc gaaactattg gtggaagcag gcgtaatcgc catgaccgcg
    12301 tttatttcgc cttttcgctc ggacaggcag cgtgcacgca atatcttccc tcatggcgat
    12361 ttcatcgaaa tctactgtga cgcaagcatt gacatctgcg aacaacgtga cgtaaagggc
    12421 ctttacaagc gggcacgaaa gggcgaggta aaggaattta cgggaatttc gtcgccctac
    12481 gaagcgcctg tcaatccgga acttatcatt cgaaccggtg aactcaccct ggaaagctgc
    12541 gtaatgcagg tcataaagta tcttgggcag cgcaatattt tcaacaacaa tccgaatgct
    12601 ctgatcggaa caaaaacaac cagtcctggc gcagcatgag agacatcctc gttaccctgg
    12661 tcgtttttgg cttgctgcct ttcgtattca aaaagccgta cgtcggcgcg ctcatgtggg
    12721 tctggatcag cgtgatgaat ccgcataccc aagggtgggg attcgcaggg accttcccat
    12781 ttgctgcgat aattgctggg gcggcgataa taagtctcat gctcaccaag gagccgaaaa
    12841 gccttccctt gacgcctgtc acggtggttt tcatcgtttt cgtcctttgg atgaacgtca
    12901 ccacggtttt aggtttttat cctgaagaat cgttcgaaca atggaacaag gtcatgaaaa
    12961 tcatgttcat gactttggtc gttctcatgc ttgtcaaaac cgaggagcat ataaaacgcc
    13021 tcatctgggt aatcgtcatc tcgcttgggt attatggagt caagggtggg atcttcacga
    13081 ttgtcagcgg tggcgtcgac atggtgttgg ggccgcaagg tacatttata ggcggaaata
    13141 acgaaatcgc gcttgcgctc attctgacca tccctctaat gcactatctg cagacaatta
    13201 ccagcagtat atggatccgt cacgctctga ctgttgccat gctactgtca gcgcttgccg
    13261 cgcttgggtc atattcccgg ggcgctttgc ttgcaatagc aatgatgggt tttttcctgt
    13321 ggcttaaaag ccgccataag ctccgcattg gcttgctctt gctgcttgcc gttccgcctg
    13381 cgatcgggtt catgccggaa aaatgggccg cgagaatgga cactataaat acttatgagg
    13441 aggatgggtc cgttctaggg cgcttcaacg cgtggtggat ggcttacaat cttgcgaagg
    13501 ataggccgct gactggcgga ggatttgaaa taatcactcc cgaccttttc tatctctacg
    13561 cgccaaatcc ggaagacctg catgctgcac acagcattta ttttcaggct ctgggtgagc
    13621 acgggtttgt tggacttggt ctataccttc tacttggctt ccttgtgtgg cgcacaggct
    13681 cttggataat aagaaatact tccggaaggg aggagtaccg atgggcctcg agccttgcca
    13741 ccatggtgca agtcagcctc gtcgggttca cggtgggagg tgcattcctc agcttgctct
    13801 attttgacgt tccttactat ctcatgtgtg cgatggtagc cactcgcatc ttggtagcga
    13861 agagattgaa agaagcgcct gcctctatcg ggcgtgatca aatcgcggat tcgaaacaaa
    13921 cacccatacc ggcccaagtc ggctcgtttg ggccatcccg atcccactcg gcggtacgcg
    13981 attaggctag agacgtcccg cgtttgttcc gctcgactgt tgatcaaagc tgcgggttaa
    14041 ttatgccaga gcaccaatct attgatctcg aggcccagcc ccggcctgtt cattccgcgg
    14101 cttttctgcc aagccaactg cttctgactc tgctttcacc tggagggcag cgcggccgtc
    14161 tgtcaacgct gatatttcac cgcgtattgc ctcgccccga tgcccttttt cctgaggagg
    14221 gcgatgccga aagcttcgag gcacaaattg cacatctggc tgcctgtttc aatatcattc
    14281 cccttcccga ggcgatacag gcattgcgga ggggaaaact gcccaagcga gccgcatgta
    14341 tcacttttga tgatgggtat
//
LOCUS       DASSHJ010000141         8948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1312234,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000141 DASSHJ010000000
VERSION     DASSHJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312234"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /gene="glnE"
                     /locus_tag="VFI43_05590"
     CDS             <1..1499
                     /gene="glnE"
                     /locus_tag="VFI43_05590"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET7061632.1"
                     /translation="QAENESVEGHLREMGYPAPEKILQRVEQFYSGVRYRQLPKASKA
                     HADALIPALIDVASRFPRAEVTLARLLDLLNNISRRATYLSLLAEHPLALERIAKLVS
                     ASEWASEYLSRHPIVMDELLNVDAFQSIPDWARFKTDLILQLDDASRSNGAGIEQQMD
                     VLRHFHHAQVFQLLAKDVEGLLPLETLSDHLTDLADLILDRVLHLAWSGLRRTHGAEP
                     AFAIIGYGKLGGKELGYASDLDIIFIYDDPHPDAPEVYARLSQRINSWLTSYTSTGLL
                     YETDLRLRPNGSSGLLVTSVEAFDEYQKNHAWVWEHQALTRARFVAGDIRVGEAFERT
                     RKEVLCQSRDLQRLKQEVLQMRRKILDAHPNSSGLFDVKHDRGGIIDVEFIVQYLILG
                     HAHDHPELTRNIGNIGLLKLSAELGLVDLETAERALRAYREFRRVQHRLRLAGISDLE
                     GSPHDKAGKFARVEPGYLSEEVAAVLRLWEEVFGPQTHLSMGETPTPG"
     gene            1743..2663
                     /locus_tag="VFI43_05595"
     CDS             1743..2663
                     /locus_tag="VFI43_05595"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET7061633.1"
                     /translation="MSMADRDGVIWSDGKVIPWRDATTHVLTHTLHYGLGVFEGLRAY
                     QTARGSAIFRLKEHTDRLFNSAHIFMMRMPFDKATLMQAQCEMVRLNNLESCYIRPIV
                     FYGSEAMGISAKTLSVHVAIAAWPWGAYLGTEGLEKGIRVKTSSFSRHHVNVNMCRAK
                     SVTTYANSILAHQEVAHDGYDEALLLDVDGYVAEGAGENVFIVKHGKLYTPDMTSCLE
                     GITRASIIELAGEIGVPVIEKRITRDEVYCADEAFFTGTAAEVTPIRELDNRTIGNGG
                     RGPVTGRLQAMFFDCVSGKSEKHASWLTYV"
     gene            2692..2898
                     /locus_tag="VFI43_05600"
     CDS             2692..2898
                     /locus_tag="VFI43_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HET7061634.1"
                     /translation="MQKEAINEDRLVEVTAEDLPLHCPMPSMLLWNSHPRVYLPIEST
                     GEAMCPYCGTRYALKGGASVGHHH"
     gene            2925..4301
                     /locus_tag="VFI43_05605"
     CDS             2925..4301
                     /locus_tag="VFI43_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7061635.1"
                     /translation="MHKIPPEIFKAYDIRGVVGKTLDSEIVKEIGHAIGTEARSRGLA
                     SIAIGRDGRLSGPELALALAEGLQASGIDVIDVGAVATPMLYFAAYTLCGYSGVMVTG
                     SHNPAEYNGLKMVLGGETLAAESIQRLRLRLEDNELMHGEGSYQKVDIAEAYLERITG
                     DIKLARPLKIVVDCGNGIAGMYAPELYRRLGCEVLELYCDVDGAFPNHHPDPSIPENL
                     RDLIETLRAADMDLGLAFDGDGDRVGVVTGDGSVIYPDRQLMLFAADVLSRNPGGRII
                     FDVKCTRNLTKWIERHGGEPIMWKTGHSFIKGKLKETNALLAGEMSGHIFFKERWYGF
                     DDGLYAGARLLELLGNQSDIDATLHALPDSVSTPELHIKLKEGENYELIAQLQKTARF
                     DNPERIITTDGLRVEYKDGFGLARSSNTMPVIVLRFEADNEASLAHIQNDFRRVILEA
                     KPDAALPY"
     gene            4451..4972
                     /locus_tag="VFI43_05610"
     CDS             4451..4972
                     /locus_tag="VFI43_05610"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET7061636.1"
                     /translation="MEEAAKTRVLFVCMGNICRSPTAEAVFRRQVEAAGLENEIYIDS
                     AGTHDYHIGEPPDERAQKAAAKRGYGTNGLRARQVDEKDFEEFHYILAMDRGNLAALQ
                     RECPPEYKHKLGLLMQYSDRFSTGSREVPDPYFGGQQGFEHVLNLVEEAGSGLLKRIQ
                     NERRQSLANSEPI"
     gene            complement(5066..7798)
                     /locus_tag="VFI43_05615"
     CDS             complement(5066..7798)
                     /locus_tag="VFI43_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET7061637.1"
                     /translation="MKRMLFNATHPEELRVAIVDGQKLIDLDIESSGKEQRKGNIYKG
                     VITRIEPSLEAAFVEYGGERHGFLPFKEISRSYFRENGDSSRTRIQEALREGQELIVQ
                     VDKDERGTKGAALTTYISLAGRYLVLMPNNPRGGGVSRRIEGEDRAELRDIMSQLDIP
                     PGMSIIARTAGIGRSEEELQWDLNYLLQLWRAIEDASNSQSGAFLIYQESSLVIRAIR
                     DYFHQEIGEILIDTESIYEQACQFMSHVMPANVGRVKLYRDDVPLFSRFQIEHQIETA
                     YSRQVTLPSGGAIVIDHTEALVSVDVNSARATRGSDIEQTALNTNLEAADEIARQLRL
                     RDLGGLVVIDFIDMEIARNQREVENRLHDSLRYDRARVQMGKISRFGLLELSRQRLRP
                     SLGESSYISCPRCHGTGHIRGTDSSALHILRIIQEEAMKENTAVLHVQVPVDVATYLL
                     NEKRAEIHAIEARLKVNVVLIPNIYFETPNYNISRLRHDDVKSGDIQVSYQRMEKPSQ
                     EVLLPSTGQEIKPARQQAAVRGIIPSQPAPIREQNAHPQQQEQAPSLLDKIFGWFKQI
                     APGETKARSEPVRSHDRIGRARIRGDRVDALERSGEASTGLNKAAGDLPKRLEKRSES
                     KPQNERSPQRKQARPPRDDKNRGEAKLPGDENVTADEVPQQEEASRRRRRGGRQRERA
                     ERPLRENKQLAPKHSHPQQVEGEAVQPEGKIEIEISQPLPELVQEAPALTLVSSGLAA
                     VATPSSSSATVSESPLVQKDVTVKPEMEQPAPGSTELPERQPENLENLGLPVQLTAEQ
                     TGNVEFAHSTSGGRETEDNGQSDQKDLEKDLDAAQPLQVEGYHETQQPLPSPIIELPD
                     LRASGLVMVETVPEKLKPAESDAGTEVAPRRRKQHLPVQIPDQDEPLVQVETHK"
     gene            8219..>8948
                     /locus_tag="VFI43_05620"
     CDS             8219..>8948
                     /locus_tag="VFI43_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7061638.1"
                     /translation="MGKEFPSRQAVKEIIKEDSDGQRVDNFLIKRLKAVPKSHIYRLL
                     RSGQVRVNSKRVEPGHHLHLGDIVRIPPVTAVEPDSPPAKTRSARIFRHVLFEVLFED
                     HCLIVINKPSGMAVHGGSGISLGIIEQLRAQHPEWKFLELVHRLDRETSGVLLLAKTR
                     TALVELHRQIREGVVGKQYLTLVKGEWQNARQSVRLPLNKFVTLAGERRVAVVTGTKV
                     EKPMAAHTVFVLKRRFQDFSLLQAE"
BASE COUNT         1981 a         2337 c         2496 g         2134 t
ORIGIN      
        1 accaggctga aaatgagagt gtcgaggggc atctacgcga aatgggatat ccagctccgg
       61 aaaaaatcct gcaacgggtg gagcaatttt attcgggggt tcgttaccga caactcccaa
      121 aggcaagcaa ggcgcacgca gacgcgctca tacctgcgct tatcgacgta gcgagccgat
      181 ttccccgtgc cgaagttaca ctcgcgcgat tactcgatct gcttaataac ataagccgac
      241 gggccaccta tctctcgctg ctggccgagc atccgctcgc gctggaacgg atagcaaagc
      301 ttgtcagtgc aagtgagtgg gcgagtgaat atctgagccg tcatccgatt gtaatggatg
      361 agcttttgaa tgtggacgcg tttcaaagca tccctgactg ggcccgtttc aaaaccgatc
      421 tcattctgca gttggatgac gcgagcaggt cgaacggggc tggtatcgaa cagcaaatgg
      481 atgtattgcg gcatttccac cacgcacagg tatttcagct gctcgcaaag gatgtcgaag
      541 ggctgcttcc actcgagacg ctcagcgatc acctcaccga tcttgccgat ctgatacttg
      601 acagggtatt gcacctggca tggtccggat tgagacgaac gcatggggcg gagcctgctt
      661 tcgcgattat cggctatggc aaactgggcg ggaaggaatt gggctacgcg tcggatctcg
      721 atattatttt tatctatgac gatcctcatc cggacgctcc tgaagtctat gctcgcctga
      781 gccagcgtat caattcctgg ctcaccagtt acacttccac cggtttgctg tacgagaccg
      841 atctgcgcct gcgtcccaat ggaagcagcg gactgctggt aacctccgtt gaggcttttg
      901 acgaatatca gaaaaatcat gcatgggtgt gggagcatca ggcattgaca cgcgctcgct
      961 tcgttgctgg cgacatccgc gttggcgaag ctttcgagag aacgcgaaag gaggtccttt
     1021 gccaatctcg tgacctgcag cgattaaaac aggaggtttt gcagatgcgt cgcaaaatcc
     1081 ttgacgctca tcctaactca agtggtttgt ttgacgtaaa gcatgaccgc ggcgggatta
     1141 ttgacgttga attcatagtc caatacctga ttctcgggca tgcacacgat catcccgagt
     1201 tgacgaggaa tatagggaat atcgggctcc tcaagctatc tgccgaatta gggctcgtcg
     1261 acttggaaac cgcggaacgg gcgctaagag cgtatcggga gtttcgccgg gttcagcata
     1321 ggctgagatt ggctggcatc tccgatctgg aggggagccc gcatgataag gcggggaaat
     1381 ttgcccgcgt tgagccaggc tatctgagtg aagaggttgc cgctgtgttg aggttgtggg
     1441 aagaagtgtt cgggccgcaa acccatctgt ccatgggaga gacgcctact cccggttagt
     1501 caaccccccg gccaccacta tgctcgacac aattcaactt aatcatggcg ctttttttgc
     1561 tctattgctt ctcgtccata acagggggca cgggaagctg cctcagtgcc gaatacagca
     1621 atcggcctcg gaacattgcg ctccggtctt ctcgctgtgg cgcataaacg accgcttcac
     1681 tctgcatttc ttttgtcaaa atgcgctaaa ctaagaggtt taaatttatc cggggctctt
     1741 aaatgtcaat ggctgaccgg gacggcgtga tctggagcga cggtaaggta atcccttggc
     1801 gtgacgcgac cacccacgta ctgacccaca ctcttcacta tgggctgggt gtttttgagg
     1861 gattgcgggc ttaccagacc gctcgaggct ctgccatttt ccgattgaag gagcataccg
     1921 accgcctttt caattccgct cacatattca tgatgaggat gcctttcgac aaggccactc
     1981 tcatgcaggc gcagtgtgaa atggtccggc tcaacaatct tgagtcctgt tatatccgtc
     2041 ccattgtatt ttacggctcg gaggcgatgg gcatatcggc taaaacgctt tcagtgcatg
     2101 tcgccattgc cgcgtggcca tggggcgcgt acctgggaac cgagggtctg gaaaaaggta
     2161 tccgcgtgaa gacctcctcg ttctcccgtc accatgtaaa cgtaaacatg tgccgcgcca
     2221 agtcggtaac cacttatgcg aactcgatac tggcgcacca ggaagttgcc catgatggtt
     2281 acgatgaagc actgctcctg gatgttgacg gctacgtggc tgaaggagcg ggagaaaacg
     2341 tttttatcgt aaagcatggc aagctttaca cgcccgacat gacctcctgc cttgaaggca
     2401 tcactcgcgc gtccatcata gaactggcgg gagaaatcgg tgtgccggtg atagaaaaac
     2461 gtattactcg ggatgaggtt tactgtgctg acgaggcttt ctttaccggc actgctgctg
     2521 aagtgactcc gattcgcgaa cttgacaaca gaacgatcgg aaacggtgga cgaggtcctg
     2581 tcaccgggag gctgcaagcg atgtttttcg actgcgtcag cggcaagtcg gagaaacacg
     2641 cgtcctggct gacctatgtc tgattcaaat ataaggggag gaaatcagcc aatgcagaaa
     2701 gaagctatca atgaagaccg tctggtggaa gttaccgcgg aggacttgcc cttgcactgt
     2761 ccgatgccat ccatgctctt gtggaattcg catccacgtg tttacctgcc catagagagc
     2821 acgggcgaag cgatgtgccc ttactgcggg acgcgctatg ccctaaaggg aggcgctagt
     2881 gttgggcatc atcattgaca tccggctcag gctcgctttt tcccatgcat aaaattccgc
     2941 cggaaatttt caaggcctac gatatcaggg gcgttgtcgg caaaacgctg gattcggaaa
     3001 ttgttaaaga aattggccat gcaatcggga ctgaagcacg gtcgcgaggc cttgcttcca
     3061 tagcgatagg cagggatggc cgattatctg gccccgaact tgcgcttgcg ttggcagagg
     3121 gcctgcaagc gagcggcatc gatgtgatag atgtgggtgc tgtcgccacc ccaatgctct
     3181 acttcgcggc ctataccctc tgcggttact ccggcgtaat ggttaccgga agccataatc
     3241 cggccgagta caatgggctg aaaatggtgc tgggtgggga gacgctggcg gcggaatcga
     3301 ttcagcgcct ccgcttgcgc ctggaggata acgaactcat gcatggcgag ggcagctacc
     3361 aaaaggtgga tatcgcagaa gcatatcttg agcgcattac tggcgatatc aagcttgcac
     3421 ggccgttgaa aatcgtggtt gactgcggca acggcattgc gggcatgtat gcgccggaat
     3481 tatatcgcag gctggggtgt gaggttctgg agctatattg cgacgtggac ggtgcttttc
     3541 ccaaccacca tccggatcca tcgatacccg aaaatctgcg tgacctcatt gagacattac
     3601 gagcggccga catggatctc gggttggctt ttgatggcga tggagaccgc gtcggggtcg
     3661 tgaccgggga cggcagcgtt atctatcctg atcgtcagct gatgctgttt gctgcagatg
     3721 tgctgtcgcg aaatccaggc ggacggataa ttttcgatgt caaatgcacg cgcaatctga
     3781 cgaagtggat cgagcggcac gggggtgagc ccatcatgtg gaaaacaggc cattccttta
     3841 tcaaaggcaa gttaaaggaa accaatgcgc tgctcgccgg tgagatgagc ggccacattt
     3901 ttttcaagga acgctggtat gggtttgatg atggtcttta cgcaggtgcg cgcctcctgg
     3961 agcttcttgg aaatcaaagt gatattgatg ccacgttgca cgcccttccc gattctgtca
     4021 gtacgcccga gctccatatc aagctgaaag agggggagaa ttacgagctt attgcacagt
     4081 tgcaaaaaac tgctcgtttt gacaatcccg agcgcattat cactaccgac gggctgcggg
     4141 ttgagtataa ggacgggttt ggcctagctc gctcgtccaa taccatgccg gtgatcgtct
     4201 tgcgtttcga ggcggacaac gaagcgtcac tcgcgcatat acagaacgat ttcagaagag
     4261 tcatcctcga ggctaaaccc gacgctgcct taccttatta gtccgcgtca cccaccagtt
     4321 actgcgttag atccgggctg tcggggcgct gcaaacagct ggaggtttta gatatacttg
     4381 gaacgccatt ctctgggatt acgacgaatg tcgctttctt ttttatataa ttttattttg
     4441 catgaccaaa atggaagaag ctgctaaaac gcgtgtcctg ttcgtgtgca tgggcaacat
     4501 ttgccgctct ccaaccgcag aggctgtttt tcggcgccag gttgaagctg cgggtctgga
     4561 gaacgagatt tatatcgatt ccgccggaac gcatgactat cacatcggcg aaccgccgga
     4621 tgaaagggcc cagaaagccg cagccaagcg aggctatgga acgaacggac tgcgggctcg
     4681 gcaggtggat gaaaaggatt tcgaggagtt tcactacatt ctggcaatgg accggggaaa
     4741 tctcgcggca ttgcagcgag agtgtccgcc ggagtacaag cataagctcg gcttgctgat
     4801 gcagtacagc gatagattct ccactggatc ccgtgaggtt cccgatccct atttcggagg
     4861 acagcaaggt tttgagcacg ttctaaatct tgtcgaggag gcggggagcg ggttgctcaa
     4921 gcgtattcag aacgaaagac ggcaatctct cgcaaatagc gagccgattt gatcgatcct
     4981 ggcaatctgc ggagtggagt gtcgttcacc ccacttctta cactccttct tacacctctt
     5041 ttctatcttt ttatcaaata aaaccttatt tgtgcgtttc tacctgcacc aacggctcgt
     5101 cctggtccgg gatttggact ggcaaatgct gttttcgccg ccgcggcgcc acttcagttc
     5161 cagcatctga ttcggcaggt ttcagtttct caggaactgt ctcaaccatg accagaccgc
     5221 tcgccctcag atcgggaagc tcgattatcg gcgatgggag cggctgctgg gtttcgtgat
     5281 agccttcaac ctgaaggggt tgtgcagcat ccagatcctt ttccagatcc ttttgatcgc
     5341 tctgaccgtt atcctccgtt tcccgccctc ctgatgttga atgcgcgaat tccacgtttc
     5401 ctgtttgttc agcggtaagc tgaacaggaa gccccaaatt ttccagattt tccggttgcc
     5461 tctcgggcaa ctcggttgat cccggcgcag gttgctccat ctcaggtttt actgtcacgt
     5521 ccttctgcac caatggagac tctgacacag tagcagaact tgaactgggg gttgccacag
     5581 ctgccagccc tgaggaaacc agcgtgaggg caggcgcctc ctgcacgagc tcgggaaggg
     5641 gttgtgagat ttcgatttcg attttgcctt cgggctgaac cgcctctcct tctacctgct
     5701 gcgggtggga atgtttcgga gccaactgct tgttctcccg caaagggcgt tctgcacgtt
     5761 cgcgctgcct accgccgcgc cgccgccgcc gtgatgcctc ttcctgctga gggacttcgt
     5821 ctgctgtgac gttttcgtct cccggcaatt tggcttctcc ccggttcttg tcatcccggg
     5881 ggggccgggc ctgctttctc tgcgggcttc tttcgttttg aggctttgac tcgcttcgtt
     5941 tttcgagccg cttcggcagg tctcctgcgg ccttgttcaa acccgtggaa gcctctccgc
     6001 ttcgctcaag cgcatccacc cgatcgccgc gaatacgcgc acggccgatt cgatcatggc
     6061 ttctcacagg ttcagatcga gcctttgttt ctcctggggc tatctgcttg aaccagccga
     6121 atatcttatc caataacgac ggggcctgct cctgctgctg cggatgggca ttttgctcgc
     6181 gtatcggcgc tggctgagaa ggaataatgc cgcgcactgc cgcctgttgc cgtgccggtt
     6241 tgatttcctg tccggtcgat ggaagcagga cctcttgtga aggtttctcc atcctttgat
     6301 agctgacctg aatatcgcct gacttgacat cgtcatgccg caaccggctg atattatagt
     6361 tcggcgtttc gaaataaata ttagggatca ggaccacgtt gactttcagc cgcgcctcga
     6421 tcgcatgaat ttcggcacgc ttttcattga gcaggtatgt tgcgacatcc acagggacct
     6481 gaacgtggag taccgcggta ttttccttca tcgcttcttc ctggattatt ctcaggatat
     6541 gtaatgcgga ggaatccgtg ccccggatat ggcccgtgcc atggcaacga gggcaggaaa
     6601 tgtaactgct ctcgccaagc gaaggcctca agcgctggcg ggacagttcc agcaagccga
     6661 agcgggaaat cttgcccatt tgaacgcgcg cccgatcata gcgcaatgaa tcgtgcagcc
     6721 gattttctac ctcccgctga ttccgggcga tttccatgtc gatgaaatcg atcacgacaa
     6781 gacctcccag gtcacgcaga cgcaactgcc tggcaatttc gtccgcagct tccagattgg
     6841 tgttaagggc ggtctgttcg atatctgaac cacgtgtagc gcgtgcagag tttacatcaa
     6901 ccgataccaa ggcttccgta tggtcgatca ctatcgcgcc accggaaggc agcgtcacct
     6961 gacgcgaata agcggtctca atctggtgct cgatctgaaa gcgggaaaaa agaggcacat
     7021 catcccggta gagcttgacg cggcccacat tggcaggcat gacgtggctc atgaactggc
     7081 acgcctgttc gtagatgctt tccgtgtcga tcaggatctc gccaatttcc tgatgaaaat
     7141 agtcacgtat cgcgcgaatg acaaggctgc tttcctgata aatcaggaaa gcgccactct
     7201 ggctattgga cgcgtcctcg atggcgcgcc agagttgcaa aagatagttc agatcccact
     7261 gcaattcctc ttcgctgcgt ccaattccgg ccgttcgggc aatgatgctc attcctggag
     7321 gaatatcgag ctgggacatg atatccctca attccgcacg atcctccccc tctatacggc
     7381 gcgatacacc tccgccgcga ggattgttgg gcatcagaac caggtagcgg ccagcaaggg
     7441 agatataggt agtgagtgcc gcccccttcg tcccgcgttc atccttgtcc acctggacga
     7501 tgagttcctg gccctcgcgg agggcctcct ggatgcgagt gcgcgaggaa tcgccgtttt
     7561 cgcggaagta tgagcgggag atttccttga agggaagaaa gccgtgacgc tcgccgccat
     7621 actcgacaaa cgcagcctcg aggcttggct cgattcgggt aattacgcct ttatatatgt
     7681 tgcctttacg ttgttccttt cctgaagatt caatatcgag atcaatgagt ttttgaccat
     7741 cgacgatagc gacacgaagt tcttccgggt gtgtcgcatt aaacagcatg cgtttcattt
     7801 tgtatgcctc ccgcgctctt gtcacgggca gacagccagc gcgcgatgtg catcagcgcg
     7861 catacgagca ttttagtcag acagcttgat catgatgtcg gggccgggtg aacgatgact
     7921 atttctctgg cttgtcaata aggccctgcc tttatagcag agtcagggaa tcaggtttca
     7981 atcagcttga aaatgcggtc cgcacgtggg acgctggtaa agctgaaaac gaaatctacg
     8041 tgtgctttga gcgcgcaaat caattactat cactacttta ttggtgagcg acgttaacca
     8101 gatcctcggg gctggctttt gccaatgcca tatcaatttg aatcaacagc gcgcttgttg
     8161 cgccggataa aggacaggcg ggaccgccga actgacaagt ataagtagaa ttaaacattt
     8221 gggcaaagaa tttccatccc ggcaagcggt taaagaaata ataaaagaag atagcgatgg
     8281 tcagcgcgtt gacaattttc taatcaagcg gctgaaggcc gtacccaaaa gccatattta
     8341 ccgactgttg cgcagtggcc aggtacgggt caacagcaag cgcgtcgagc cgggacatca
     8401 tcttcacctc ggcgatatcg tgcgaatccc gccggtcact gcggttgaac cggattctcc
     8461 ccccgccaaa acgcggagcg cgagaatctt ccggcatgtg ctgttcgagg tgctgttcga
     8521 ggatcactgc ctgatagtca tcaacaagcc atccgggatg gcggttcatg gtggaagtgg
     8581 aataagcctg gggattatcg agcagctacg ggcgcagcat ccggagtgga agttcctgga
     8641 actggtgcac cgcctggaca gggaaacctc gggagttctg ctccttgcaa agacaagaac
     8701 cgcgctcgtt gagctgcaca ggcagatacg cgaaggagtc gtggggaagc agtacctgac
     8761 gcttgttaaa ggtgaatggc aaaacgccag gcaaagcgtg aggttgcccc tcaacaaatt
     8821 cgtaaccttg gcgggggaaa ggcgtgtagc cgtggtaact ggaacaaagg tagagaagcc
     8881 gatggccgcg cataccgtgt tcgtgctaaa gaggcgcttt caggatttca gtctgcttca
     8941 ggcggaac
//
LOCUS       DASSHJ010000142        13716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_151764, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000142 DASSHJ010000000
VERSION     DASSHJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151764"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1213
                     /locus_tag="VFI43_05625"
     CDS             2..1213
                     /locus_tag="VFI43_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET7061639.1"
                     /translation="MSTFYPPQRTLMGPGPSDTHPRVLSAMARPTLGHLDPVFTEMME
                     ELKTLLRYAFQTSNLLTFPVSGPGSVGMEMCFVNMINPGDKVIVCRNGVFGGRMIENV
                     QRCRGTAVVVEDPWGAPVDPQKVEDALKQNPDAKIVAFVHAETSTGALSDAKTLCEIA
                     ERHNCLTIVDTVTSLGGTPVKVDEWKIDAIYSGSQKCLSCPPGLSPVSFSERVVDLVK
                     NRKGKVQSWFMDLNLLLGYWGTTRTYHHTAPTNALYALHEALVMLYEEGLEHSWARHQ
                     RNHTAFKAGLETFGIQYLVEEKARLPQLNSVYVPEGIDEKEVRRRLLADYSLEIGAGL
                     GDLAGKIWRFGLMGYSSRIENVMLCLGALETVLSDMGMKVEQGAAEAKAHYAYAANPL
                     PVESKSAAKAA"
     gene            complement(1249..2094)
                     /gene="metF"
                     /locus_tag="VFI43_05630"
     CDS             complement(1249..2094)
                     /gene="metF"
                     /locus_tag="VFI43_05630"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET7061640.1"
                     /translation="MESQKKLPPTFSFEFFPPQTAEGVEKLRATRRQLAQLKPKFFSV
                     TFGAGGSTRDRTLATVLEIQNEGYPVAPHLSCIGSTSENIRTILQRYSDNGIRHLVAL
                     RGDLPSGMAQGGEFRYADELVAFIRREFGSTFHIDVASYPEYHPQASSAQDDLQNFKR
                     KVEAGADSVITQYFYNADAYFSFVDACEEMGIDIPIVPGIMPINKYSQLARFSDACGA
                     EIPRWIRKKLEGYGDDSASIRAFGLDIVTDLCDRLLSAGAPGLHFYTLNSAGLTTTIW
                     QRLGL"
     gene            complement(2161..3645)
                     /gene="ahcY"
                     /locus_tag="VFI43_05635"
     CDS             complement(2161..3645)
                     /gene="ahcY"
                     /locus_tag="VFI43_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7061641.1"
                     /translation="MNAVLSASKPATGPNVTSSTPSSKSGEFSDYKVADLSLAEWGRK
                     EIAIAETEMPGLMALREEYAAAKPLTGACIAGSLHMTIQTAVLIETLISLGAQVRWAS
                     CNIFSTQDHAAAAIAAAGIPVFAYKGESLDDYWEFTHRILEWPGETSSTGGPNMILDD
                     GGDATLLIILGSKAEKDPSVIANPTNEEEQALFASIKKRLSTSPNWYSNKLANIRGVT
                     EETTTGVHRLYEMEKKGELPFPAFNVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIA
                     GKIALVCGYGDVGKGCAQSLRGLGATVWITEIDPICALQAAMEGYRVVTMDEVCDQAD
                     IFVTATGNLRVITHDHMLRMKNQAIVCNIGHFDSEIDIASIQKYQWENIKPQVDHVIF
                     PTGRRIIVLAKGRLVNLGCATGHPSFVMSSSFTNQVLAQMELWQNGESYQKNVYVLPK
                     HLDEKVARLHLGKLGVKLTELTEEQANYLNMDKNGPYKPALYRY"
     regulatory      complement(3660..3737)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(3795..4982)
                     /gene="metK"
                     /locus_tag="VFI43_05640"
     CDS             complement(3795..4982)
                     /gene="metK"
                     /locus_tag="VFI43_05640"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7061642.1"
                     /translation="MNDHLFTSESVSEGHPDKVADQISDSVLDAILAQDPNARVACET
                     LCSTGLIVMSGEITTQANVDYMQVARAAVKSIGYNSSDIGFDYRTCAVLTAFNKQSPD
                     IAQGVNRSKEEEMDQGAGDQGLMFGYACDETPQLMPMPIYYAHRLMERQAELRKDGRL
                     PWLRPDAKSQVSVRYLNGKPQHIETVVISTQHHPDISHTQLSEAVIEEIIKPVLPKKM
                     LSSETRYLINPTGRFVVGGPMGDCGLTGRKIIVDSYGGTAHHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVSAGLASRCEVQVAYAIGVARPVSLMVDTFGTGKIADDKIVKLIER
                     HFDLRPRAIIHTLNLLRPIYQKTAAYGHFGRDEPEFTWEATDKAARLRADAGMKVAAE
                     APA"
     gene            5111..5938
                     /gene="dapF"
                     /locus_tag="VFI43_05645"
     CDS             5111..5938
                     /gene="dapF"
                     /locus_tag="VFI43_05645"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7061643.1"
                     /translation="MKLKFTKMQGLGNDFVVIDGVNQSISLDTATLRTLADRRFGVGC
                     DQILVVERPQGDADFRYRIFNADGGEVEQCGNGARCFARYVHDRGLTKKTQIRVETRA
                     GLIVPRLEPGGEVSVNMGVPRFEPREIPFIAEKRSFTYLLDIGDKQVEISAVSMGNPH
                     AVQCVDDVENYAVAQEGPRVEMHPRFPERVNAGYMQVLDRHRVKLRVYERGSGETLAC
                     GTGACAAVVAGIERGLLEPDVEVNFRGGKLSVSWEGKGHPVWMTGPAVAVFDGEICL"
     gene            5976..6653
                     /locus_tag="VFI43_05650"
     CDS             5976..6653
                     /locus_tag="VFI43_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HET7061644.1"
                     /translation="MTFNDEEIARYLKDNPGFFEQYGAMLADVQVPHPRSGDAIPITE
                     RQIVALREKNRMLQEKLSELIRFGEENGALIEKMHRLAVALLVSRRLDALLNTFEFHL
                     REDFAVPHVGLRVWNMTGGRIILPQFARTSGDIHQMADSLIDPYCGHHVVEEIKSWFG
                     EGAGRLRSFAMVALRAKGQAMGLLVLASEDPQRFYAGMGTVYLAQLGEFLSAGLERFM
                     LADAVDS"
     gene            6671..7582
                     /gene="xerC"
                     /locus_tag="VFI43_05655"
     CDS             6671..7582
                     /gene="xerC"
                     /locus_tag="VFI43_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET7061645.1"
                     /translation="MNLDKDNTLAAEYLTHLATERRLSPRTCASYSHDVRILLDLAKD
                     TSPQDISLNQLQVHHIRRFIALLHGKGLSGKSLARLLSAWRGFYKYLARNQGHVTNPC
                     AGVRAPKSAKTLPRTLSPDEAGKLLEFSVDGDVTALRDKAMFELCYSSGLRLAELAGL
                     KPEDLDFSDGTARVNGKGGKTRIVPVGSKAMEALRNWIRHRANLAKPGEVALFLSRYG
                     RNISHRSIAQRLKVQAVRQGLDSTVHPHMLRHSFASHILQSSGDLRAVQEMLGHASIR
                     TTQVYTHLDFQHLSKVYDAAHPRAKKS"
     gene            7722..8258
                     /gene="hslV"
                     /locus_tag="VFI43_05660"
     CDS             7722..8258
                     /gene="hslV"
                     /locus_tag="VFI43_05660"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET7061646.1"
                     /translation="MPEFHGTTILSARRGAQVALGGDGQVTLGAVVAKASARKVRRLY
                     HDKILAGFAGGTADAFTLFERFEGKLEKHQGHLMRSAVELAKDWRTDRILRRLEAMLV
                     VADPGVTLIITGSGDVIEPELGLAAIGSGGSYAQSAARALLENTSLSPVEIVKKSLAI
                     AGDLCIYTNQNHVIEVLE"
     gene            8332..9663
                     /gene="hslU"
                     /locus_tag="VFI43_05665"
     CDS             8332..9663
                     /gene="hslU"
                     /locus_tag="VFI43_05665"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET7061647.1"
                     /translation="MSQLTPREIVHELDKHIIGQDAAKRSVAIALRNRWRRQQVNDPL
                     RQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LVESAIKQAREQETQKMRARAEDNAEDRILDALLPPARELGAQHEAVEGESATRQKFR
                     KKLREGELNDKEIEIEVASPHAHMEIFAPPGMEELTTQIQGMFQNIGGERKKTRKLRI
                     REAMKLIIEEEASKLINDEELKLRSVQNVEQNGIVFLDEIDKITSRSETSGADVSRQG
                     VQRDLLPLVEGTTVSTKYGMIKTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELAS
                     LSADDFVQILTNTDACLTRQYEALLETEGVKLEFTPGAVRRLAEIAYAVNEKTENIGA
                     RRLYTAMEKLLEDVSFEAEKHHGTNVVVDAEYVDLRLSSLSQSEDLARYVL"
     gene            complement(9854..10501)
                     /locus_tag="VFI43_05670"
     CDS             complement(9854..10501)
                     /locus_tag="VFI43_05670"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET7061648.1"
                     /translation="MSNPILYTMPGTCALAPNIAVAWLNAPVMIKTIERGDQKKEEYL
                     SINPKGKVPALQFDDGDVLTEAAAILAYLGAEYGKEGYARNQKLGRKEAEALSYMTSE
                     VHATYGPHFAPQAFADSESAQKEIKAKTYEKLATHYARLEGNLAAAGDWYLKDRSFAD
                     AYLYVLTRWIEQTPLSIDDYPNLKKHRARMEADEGVKLALQRQNMEPVSYPAKNR"
     gene            complement(10770..12029)
                     /locus_tag="VFI43_05675"
     CDS             complement(10770..12029)
                     /locus_tag="VFI43_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HET7061649.1"
                     /translation="MSHYDENQHNIAETPDPLTKASPAATSTTEVATAVRISDRRPSN
                     AASQTQCQSEHDREIPEVDLMSPLTLGSVTFRNRIAMSPMCMYSAHDGFANDFHLVHL
                     GSRAIGGVGLVVVEATAVTAEGRISPEDMGIWKDEHIEPLARIARFVEMHGAVPGIQL
                     AHAGRKASCDVPWNGGRSLETPAGGGWPVIGPSPLPFHADDPIPKPMSETDIEYCIEA
                     WETGARRALDAGFKVIELHAAHGYLMHEFLSPLSNHRTDNYGGSLANRVRLLLMVAKR
                     LRDVIPSELPFFVRISATDWVNGGWDIEQSIILCRELKLLGVNLIDVSSGGAIPDARI
                     PVARGFQVPFARRIRDEAQIHTGGVGLITDPQFANEIITSGEADLVFIGRELLREPYW
                     VLKAQHMLGIEPEWPIPYGYAVKRRAK"
     gene            complement(12488..13294)
                     /locus_tag="VFI43_05680"
     CDS             complement(12488..13294)
                     /locus_tag="VFI43_05680"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HET7061650.1"
                     /translation="MNNLHRELAPISGKAWAQIEEETARTLKRHFAARRVVDVPEPKG
                     LQLSAVSTGHLHQIQAPGDGIQAVQRDVKAIVELRVPFELARQAIDDVERGATDSDWR
                     PMKDAARKIAFAEDRAIFNGYAAAGIQGIREGSSNSEVVLPSSVRGYPDAVAQAVSQL
                     RLAGVNGPYALVLGTDAYTAASGGSDEGYPVFNHIERVVDGGVVWAPAIEGGFVVTTR
                     GGDFKLDIGQDISIGYSGHSSTDVELYFLETFTFRLLTTEAAVVLTLGEK"
BASE COUNT         3036 a         3663 c         3779 g         3238 t
ORIGIN      
        1 gatcagcacg ttttaccctc cccaacgcac actgatgggt cctggcccat ccgataccca
       61 cccgcgcgtg ctttctgcca tggcccgtcc gacactaggt cacctcgacc ccgtttttac
      121 ggaaatgatg gaagagctga agactcttct gcgttacgcc ttccagacat ccaacctgct
      181 gaccttcccg gtctcgggcc cgggctccgt tggcatggag atgtgcttcg tcaacatgat
      241 caaccctggc gacaaggtga tcgtttgccg taacggcgta ttcggcgggc gcatgatcga
      301 aaacgtgcag cgctgccgag ggacggctgt cgtcgtggaa gacccgtggg gcgctccggt
      361 cgatccgcag aaagttgaag atgctttgaa acagaatccc gacgcaaaaa ttgttgcttt
      421 cgttcatgcc gagacttcca ccggagcctt gtcggatgcc aagactttgt gcgaaatcgc
      481 cgagcgccac aattgcctga ctattgtcga tacagtcaca tctctgggcg gaactcccgt
      541 aaaggtcgat gaatggaaga tcgatgcaat ctattccggg agccagaaat gtctctcatg
      601 cccgcctggt ttgtcgcccg tcagcttttc ggagcgggta gtagatttgg tcaagaatcg
      661 taaaggaaaa gtgcagagct ggttcatgga tctgaatctg ctgctaggat actggggtac
      721 tacccgcacc tatcaccata ctgcacccac caacgcactg tacgccttgc acgaagcgct
      781 ggtaatgctt tacgaagaag gcctggagca ctcatgggcg cgtcatcagc gcaatcacac
      841 tgcgttcaag gcagggttgg aaacattcgg catccagtac ctggtggaag agaaggctcg
      901 cctgccccag ctcaattcag tgtatgttcc tgaaggtatt gatgaaaagg aggtccggcg
      961 cagactactg gcggactata gccttgaaat cggtgcaggg ctgggcgatc ttgcaggcaa
     1021 aatctggcgt tttggcttga tggggtattc cagcagaatc gaaaacgtca tgctttgtct
     1081 gggcgcgctc gaaaccgttc tgtctgacat gggaatgaag gtcgagcagg gagcggccga
     1141 agccaaggcg cattacgctt atgcggccaa ccctcttcct gtcgaaagca agagcgcggc
     1201 aaaggcggct tgaggttcag aggctatcta ctggcaggct gggtgttgtt acaatcccag
     1261 cctctgccat atcgttgtcg tcaatccggc tgagttgagc gtataaaagt gcagtccggg
     1321 tgccccggca ctgagcagcc gatcgcatag atcggttacg atatccaatc cgaaggcgcg
     1381 gatcgacgcg ctgtcgtccc cgtagccttc cagttttttt cttatccacc tcggaatttc
     1441 cgcgccgcat gcatccgaga agcgggcaag ctgcgaatac ttgttgatgg gcatgatccc
     1501 cggcacgatg ggtatgtcaa tgcccatctc ctcgcaggca tcgacgaagc tgaaataggc
     1561 gtcggcgtta tagaaatact gcgtgatcac cgaatccgca cctgcctcaa ccttgcgctt
     1621 gaagttctgt aaatcatcct gcgctgaact ggcctgggga tgatattccg gatatgacgc
     1681 aacatcgatg tggaacgtgc tgccgaattc tctgcggatg aatgccacca gctcatcggc
     1741 ataacggaat tctccgcctt gcgccattcc cgacgggaga tcgcctctca aggcaacgag
     1801 gtggcgaatt ccgttgtcgg aatatctctg caatatggta cggatattct cagaggtcga
     1861 gccaatgcag gagagatggg gcgcgacagg gtatccttcg ttctggatct ccagtacggt
     1921 cgcaagcgtc cggtcacggg tggatccacc cgcaccgaat gtcaccgaaa aaaacttggg
     1981 cttgagttgc gcgagttgcc tgcgcgtggc gcgcaacttt tctacacctt cagcggtttg
     2041 cggtggaaaa aattcgaaac tgaaggtagg agggagcttc ttctgcgatt ccattttgcg
     2101 agcaggggaa aggagagccg ccagggttcg gccctccttt acgtcttcct tataggaaaa
     2161 tcagtaccgg tagagagctg gcttgtaggg accgttcttg tccatattta gatagttcgc
     2221 ctgctcctcg gtcaactcag tgagcttgac gcccagcttt ccgagatgga gccgcgcaac
     2281 tttttcgtcc aggtgcttgg gcagaacgta aacgtttttt tggtagctct cgccgttttg
     2341 ccagagctcc atttgcgcca gtacttgatt cgtgaacgaa ctggacatga caaatgaggg
     2401 atggcccgta gcacatccaa gatttacaag gcgtccttta gccagcacga tgatgcggcg
     2461 gccagtgggg aatatcacat ggtccacctg cggcttgata ttttcccact ggtatttctg
     2521 aatggaggca atatctattt cggaatcgaa gtgcccgata ttgcagacga tagcctgatt
     2581 cttcatgcgc aacatatggt catgcgtaat gacgcgcaga ttaccggttg cggttacgaa
     2641 tatgtctgcc tgatcgcaga cctcgtccat cgtgacgacg cgataacctt ccattgcggc
     2701 ctgcagggca cagataggat ctatttccgt gatccaaacg gttgcaccca gcccccgcag
     2761 cgattgcgca caccctttgc ccacatcgcc ataaccgcac accagcgcga tcttgccggc
     2821 gatcatgacg tcggtggccc gtttgatgcc atccacgagc gattcgcggc aaccatagag
     2881 attatcgaat ttcgatttgg taacggagtc gttgacgttg aacgccggga acggcagttc
     2941 gcctttcttt tccatttcat acaggcgatg cacgcccgtg gtcgtttcct ccgttacacc
     3001 ccggatgttg gcaagtttat tggaatacca gttgggactt gtgcttaacc tttttttgat
     3061 ggatgcgaac agcgcctgtt cttcctcatt tgtcggattc gcaatgacag agggatcctt
     3121 ttcggcttta ctcccaagga tgatcaggag cgtcgcatcg cccccgtcat ccagaatcat
     3181 gttgggtccc cctgtactgg aggtttcgcc gggccattcg aggatgcggt gggtgaattc
     3241 ccaataatcg tccagggact cacccttgta ggcaaacaca gggattccgg ccgccgcgat
     3301 agcggcggcg gcatgatcct gggttgaaaa gatattgcag gaagcccatc ggacttgagc
     3361 tcccaagctg atgagggttt cgatcagcac cgccgtttga atggtcatgt gcagggaacc
     3421 tgcaatacaa gcgccggtga ggggttttgc agccgcgtat tcttcccgta aagccatcag
     3481 gccgggcatc tcggtttcgg cgattgcaat ctctttgcgc ccccattccg caagagataa
     3541 atcggcgact ttataatccg agaattcgcc cgatttactg gaaggggtcg atgatgtaac
     3601 gttggggcca gtggcaggct tcgacgcgct aagcacagcg ttcataaaaa tatctccaaa
     3661 atgaacgagc gccgttgttg caatccttcc tctgccgagc ctcgcgggat atgcccgttg
     3721 cagcgctcct cggcggagga aggggttaag ctgtttaaat cagggtgcaa acctggtatg
     3781 ccggggaggc attatcatgc tggagcctcg gctgcgacct tcattcctgc gtctgcccgc
     3841 aggcgcgcag ctttgtcggt tgcctcccag gtgaattccg gttcatcccg gccgaaatgg
     3901 ccgtacgcgg ctgttttctg atagatcgga cgcaagagat tgagcgtgtg gataattgcg
     3961 cgcggacgca ggtcgaaatg ccgttcgatc aacttgacaa tcttgtcatc cgcaatcttg
     4021 cccgttccga aagtatcaac catcaaggaa acggggcgag ccacgccaat cgcatacgct
     4081 acctgaactt cgcaccggct cgccaaaccc gcagacacga tgttcttcgc cacatagcgg
     4141 ccggcatagg cggcggagcg gtctaccttt gacggatcct tgccggagaa ggcccctccc
     4201 ccatgatgtg ccgtccctcc ataactgtcc acaatgattt tgcgtccagt caagccgcaa
     4261 tcccccattg ggccgccgac cacaaagcgt cccgtcgggt tgatgaggta gcgggtttcg
     4321 ctactcagca ttttcttcgg gagaacaggt ttgatgattt cctcaatcac agcttcgctc
     4381 agctgcgtat gcgaaatgtc gggatggtgc tgcgttgata tcacaactgt ttcaatatgc
     4441 tggggtttgc cgttcaaata gcgcaccgat acctgagact ttgcgtccgg gcgcagccag
     4501 gggaggcggc cgtcctttct gagctccgcc tggcgttcca tgagccgatg ggcatagtat
     4561 atcggcatgg gcatcaactg cggcgtctcg tcacaggcat agccgaacat gagcccttga
     4621 tctcccgcgc cctgatccat ctcctcttcc tttgagcgat tgaccccttg ggcaatatcc
     4681 ggagattgtt tattgaaggc ggtcagcaca gcgcaggtac gatagtcgaa tccaatatca
     4741 gagctgttgt aaccgatgct cttgacagcg gcacgtgcga cctgcatata gtcaacattg
     4801 gcctgggtcg tgatttctcc cgacatgacg atcagaccgg tgctgcatag cgtctcgcaa
     4861 gcaacgcgcg cgttagggtc ttgcgcaaga attgcatcga ggaccgagtc ggaaatctga
     4921 tcggcgacct tgtcaggatg gccttctgat acggattcgg aagtaaaaag atgatcattc
     4981 atgttgttcc cgccgcttat tgtctttgag gcctgtagaa taccaaatat tccttccatt
     5041 aagaagatag agaagatagg gaatatcagt ccagactgtt cgaatcattc agcgacctct
     5101 tcatcgaatc atgaagctca aatttaccaa gatgcaggga ctgggcaatg attttgtcgt
     5161 gatcgatggc gtgaatcaat ccatttccct tgacaccgcc acgttgcgta cgctggctga
     5221 ccgccgcttc ggggtcggct gtgatcagat cctcgtggtt gagcggcccc agggggacgc
     5281 ggatttccgc tatcgtattt ttaatgcgga cggtggcgag gtagaacaat gcggcaacgg
     5341 ggcccgttgt ttcgcgcgtt atgtacatga ccggggattg acgaagaaaa cccaaatacg
     5401 agtcgagacg cgtgccgggc tgatcgttcc caggttggag ccgggaggag aggtaagcgt
     5461 caatatggga gtgccacgat ttgaaccgcg agaaatcccc tttattgctg aaaaacgttc
     5521 gttcacatat ttgctcgata tcggtgataa gcaggttgaa ataagcgcgg tctccatggg
     5581 caacccccat gccgtgcaat gcgtggacga tgtggaaaac tatgcggtcg ctcaggaagg
     5641 cccccgggtt gagatgcatc cgcgctttcc agagagagta aatgcgggat acatgcaagt
     5701 gctggatcgc catcgtgtta aattgcgtgt ttatgaacgc ggttctggcg aaacccttgc
     5761 ttgcggaacg ggcgcttgtg cggcggtcgt tgcagggatt gagaggggat tgctggagcc
     5821 cgatgttgaa gtcaattttc gcgggggcaa gctgtctgtc tcctgggaag gcaaaggtca
     5881 tcccgtctgg atgaccggtc ctgccgttgc tgttttcgac ggggaaatct gtctctgagc
     5941 aggatgacag ggattaaggg aattcagata acaatatgac tttcaatgat gaagagatag
     6001 cccgatactt aaaggacaac cccgggtttt ttgaacaata tggcgccatg ctggccgatg
     6061 tacaggtgcc acatccccgt tccggggacg ctatcccgat caccgaacgc cagatcgtcg
     6121 ctctccgcga aaaaaaccgc atgctccagg aaaagctaag cgaactcatc cgatttggtg
     6181 aagagaatgg agctctcatt gaaaaaatgc accgtcttgc tgttgcgctt cttgtttccc
     6241 ggcgtctgga cgcgttgctc aatactttcg agtttcattt acgggaagac ttcgcggtcc
     6301 cccacgtggg gctacgggtc tggaacatga ctggcggtcg gatcattctg ccgcaattcg
     6361 ccagaacgag tggcgatatc catcaaatgg cagacagcct gatagatcct tattgcggcc
     6421 atcacgtagt ggaggaaata aaaagctggt ttggggaggg tgcgggacgg ttgcgctcgt
     6481 ttgcaatggt ggctctacga gcaaagggcc aggctatggg cttgcttgtc ctggcaagcg
     6541 aggatcccca acgcttttat gcaggaatgg gaacagtcta tctcgctcag ttgggggaat
     6601 ttttgagcgc cgggctggag aggttcatgt tggccgatgc cgttgattct tgaagcagtg
     6661 ggttgcccct atgaacctgg ataaagacaa caccctcgcc gccgagtacc ttacgcatct
     6721 tgcgacggaa agacgattgt ctccgcgtac ctgcgcgagc tactcgcatg acgtccggat
     6781 cctgcttgat cttgccaaag acacttctcc tcaggacatc tctctcaatc aacttcaagt
     6841 tcaccatatc cggcgcttta tcgctttgct acatggcaaa gggttgtcag ggaaaagtct
     6901 cgcgcggcta ctgtcagcct ggcgaggctt ctacaagtac ctggcacgga accagggcca
     6961 tgtaacgaac ccttgtgccg gagtgcgtgc gccaaagtca gcgaagacat tgcctcggac
     7021 gctttcaccg gatgaggccg ggaagctgct tgagttttcc gttgatggtg acgtgacggc
     7081 cttgcgtgat aaggcaatgt tcgaactttg ttattcatcc ggtttacggc ttgcggagct
     7141 ggccggtttg aagcccgaag acctggattt ttcggacggc accgcgcggg taaacggcaa
     7201 gggtggaaag acgcgaatcg tgcctgtcgg cagcaaggca atggaagcgc tgaggaattg
     7261 gataaggcat cgcgcgaatc tggcaaaacc gggcgaggtg gcattattcc tttcgcgata
     7321 cggcaggaat atcagccatc ggtcgattgc ccaacggctg aaggttcaag cagtgcgcca
     7381 aggcctcgac tcgactgttc atcctcacat gctgcggcat tcgtttgcgt cccacatact
     7441 ccagtcgagc ggcgatctgc gagccgtgca ggaaatgctt gggcatgcaa gcattcgcac
     7501 tacccaggtt tatacgcatc tggattttca gcacttatcc aaagtctatg atgcagccca
     7561 cccgagggcg aagaagagtt agattcctta agttgtcatg cgagtgtggg atttccagga
     7621 atcagcgtta taatttgacc cgcccgagag caagtctcta gggcttgcgg aatattcatt
     7681 caaacgtttc gtggctcctc cgaccacttt ttaatagtcc aatgccagaa tttcacggta
     7741 ctacaatact ttccgcccgg cgcggcgccc aagttgcgct tgggggcgat ggccaggtaa
     7801 ctttaggtgc ggtggtggcg aaggcgagcg cccgaaaggt tcgaaggctt taccacgaca
     7861 aaatactcgc ggggtttgcg ggtggcacgg ccgacgcctt cacgctgttt gagcgttttg
     7921 aaggaaagct tgaaaaacat cagggccacc ttatgcgctc ggccgttgag ttggcgaaag
     7981 actggcggac cgaccgtatt ttgcgtcgtc ttgaagcgat gctcgtcgtg gcggatccgg
     8041 gagttacgct catcataacg ggttcgggcg acgtgatcga gccggagctt ggccttgcgg
     8101 caattggcag cggtggctct tatgctcaat cagccgcgcg tgcgcttctc gaaaatacca
     8161 gcttgtcccc ggtggaaatc gtcaagaagt cgcttgcgat cgccggtgac ctgtgtatct
     8221 ataccaacca gaaccatgta atagaggtcc tggagtagcg caaggcggat tacctctggt
     8281 tttctctttc cgccttcagt tttctgcttc caaatcaata ctgggtaacc aatgtctcaa
     8341 ttgacgcctc gggaaatcgt ccacgaactc gacaaacata tcataggcca ggatgctgcc
     8401 aagcgttccg tagctatcgc actcaggaat cgctggcgca gacaacaggt taatgatccc
     8461 ctccgccagg agatcacgcc taagaatatt ttgatgatcg ggcctacggg agtgggtaaa
     8521 actgaaattg cccggcgatt ggcgaaactt gccgatgcac catttatcaa ggtggaagcg
     8581 accaagttta cggaagtcgg gtacgtgggg cgcgacgtgg attcaattat ccgggatctg
     8641 gtcgagtcgg cgatcaagca ggcgcgcgag caggaaacgc aaaaaatgcg cgcgcgggcg
     8701 gaagataatg ctgaagaccg catcctggat gctttgcttc ctccagcccg agagcttggg
     8761 gctcagcatg aagcggtcga aggggaaagc gcgacgcgtc agaaattccg caaaaagctg
     8821 cgtgaaggcg agctcaacga taaagagatt gaaatcgaag tcgcttcgcc tcatgcccat
     8881 atggagatat ttgccccgcc tggaatggaa gaactgacta cccagataca ggggatgttc
     8941 cagaatatcg ggggtgagag aaaaaaaacc cgcaagctgc gaatccggga ggcgatgaag
     9001 cttatcattg aagaagaggc gtccaaactc atcaatgacg aggagctcaa actgcgttct
     9061 gttcagaacg tggagcagaa cggcattgta tttctggacg agatcgacaa gatcacaagc
     9121 cgctcggaaa cttcaggggc cgatgtttcc cgccaggggg ttcaacgcga tctgctgcct
     9181 ctggtggaag gcacgacagt ttcaacgaaa tatgggatga taaaaaccga ccatatttta
     9241 ttcattgcca gcggcgcgtt ccaccttgcc aagccctccg atctgatacc cgagttgcag
     9301 gggcgctttc ccattcgggt cgagttggca agcctgagcg ctgatgactt tgtgcaaatt
     9361 ctcacaaaca ccgacgcctg cctgacccgg cagtacgagg cgctgctgga gaccgaaggg
     9421 gtcaagctcg agtttacacc cggtgcagta agacgcctgg cggaaatcgc ttacgcggtt
     9481 aacgagaaaa cagagaatat cggcgcccgc cggctttaca cggcgatgga aaagcttctg
     9541 gaagacgttt ccttcgaagc ggaaaagcat catggaacaa atgtcgtggt cgacgccgag
     9601 tacgtggatc tgcggttgag cagtttgtcc cagagcgagg atttggcgag atacgtgctt
     9661 tgaaaacgcg aagcattggt tgacgcctgt ttaagtcgcc atcttaaatt gtcgaatatt
     9721 ccgcttagct ggaagtcgct cgtctcgtca agctgcttga tgagctagca ttttaatctc
     9781 aggcgttccc taaaattccc tcctcgttcc gatgcgattc cccctttgat ctttcggcaa
     9841 aaccggcgcc catttatcgg ttttttgcgg ggtatgacac tggttccata ttctggcgtt
     9901 gcaaagccag cttcacgcct tcatcggctt ccattcgtgc acgatgtttc ttcagattcg
     9961 gataatcgtc aatcgataaa ggggtctgtt cgatccatcg cgtgagtaca tagagatagg
    10021 catcagcaaa acttcgatcc ttcaggtacc agtcaccggc ggctgctaga ttcccctcca
    10081 ggcgtgcgta atgcgttgcc agtttttcat acgttttggc cttgatttct ttttgtgccg
    10141 actcgctgtc tgcgaatgcc tgtggtgcaa agtgtgggcc ataggttgcg tgaacttccg
    10201 aagtcatgta gctcagcgct tcggcctcct ttcgccccag cttctgattg cgcgcatagc
    10261 cctctttgcc gtattcggcg ccgagatacg ccagaatggc tgcagcttca gttaatacgt
    10321 cgccgtcgtc gaattgcagc gctggcacct tgcctttcgg gttgatagac aaatactctt
    10381 cttttttttg atctccacgt tcgatcgttt tgatcattac aggcgcattc agccacgcga
    10441 ccgcaatgtt gggtgccagt gcacaggtac ccggcatggt ataaagaatg ggattactca
    10501 tcacacgctc ctaaagttca ttggcgactc ctgaacacga tttgatctgg caaaacttcc
    10561 caacctaagc tgtcctgttt atggttaatc tttgtcgatt catatcaccc cacttgatat
    10621 ggctccctga atccttacca ttcggcgcaa tctctaactt gaagcttgcg tcaaactgcc
    10681 tgcctaccct cagtttcatc ttcaaacctt ctctcaaaaa tgaataattc actcatcgag
    10741 cgtctgtgcc aattaagcca atacaatacc tattttgccc gccgcttgac agcataaccg
    10801 tacgggatag gccattcggg ttctatccct aacatgtgtt gcgccttgag aacccagtaa
    10861 ggttcccgca gtaactcacg gccgataaaa acgagatcgg cctcgccgct ggtgatgatc
    10921 tcattcgcga attgcgggtc agttatcagt cccactccgc ccgtgtggat ttgtgcttca
    10981 tctcgaatgc gtcgggcaaa tggaacctgg aaacctctgg caacgggaat ccttgcatcc
    11041 ggtatcgcgc cgcccgacga gacatcgatc agattcaccc caagtaactt cagttctcgg
    11101 caaagaataa tcgattgttc tatatcccac cctccattga cccagtccgt ggcggagatt
    11161 cgcacgaaga aaggcagttc ggacgggata acatcgcgca ggcgcttggc aaccattagc
    11221 aaaagccgca cgcgatttgc caggctgccg ccataattat ccgtgcgatg attgctcaaa
    11281 ggcgatagaa attcatgcat caggtagccg tgtgccgcgt ggagttctat cactttgaag
    11341 cctgcgtcca gcgcccgccg agcgcctgtt tcccatgcct caatgcaata ttcaatatcc
    11401 gtttccgaca tgggctttgg aatcgggtcg tcggcgtgaa aaggcagggg gctgggacca
    11461 atcaccggcc agcctcctcc cgctggagtt tccaggctgc ggccgccatt ccacgggaca
    11521 tcgcaactgg ctttgcggcc ggcatgtgcg agctggatgc ctggaaccgc gccatgcatc
    11581 tcgacaaacc gcgcaatgcg agccagcggc tcgatatgct catctttcca gattcccatg
    11641 tcttccggtg aaatgcgtcc ctcagccgtg actgcagtcg cctcaacaac cacaagccct
    11701 acgccgccga ttgcccggct accgagatgg acgagatgga agtcattggc aaaaccgtcg
    11761 tgggcggaat acatgcacat gggtgacatt gcgatccggt tgcggaaagt aacgctgccg
    11821 agggtaagcg gcgacatgag atccacttcc gggatttctc ggtcgtgctc gctctgacat
    11881 tgcgtctgac tagcggcatt gcttggccgg cggtcgctga tccggacggc tgttgcaact
    11941 tctgtggtgg aggtggcggc aggacttgcc ttggtcagag ggtcaggagt ttcagctata
    12001 ttgtgttggt tctcatcata atgagacatg actttttcct catttaagaa ttgccagtga
    12061 tcctcctgat ctcctgaata tcgtagccaa tattgcgccg gtactcattc ttgaaaaacc
    12121 gtagtccgag cgattgttac gcacggcaag cgccgcttct tcagagaaac tctagagccg
    12181 gtgcgccgct ttcctggagg aagccgtatc tcttatcatc tcgttgctga tacggtcaag
    12241 tgcgtgatga aaggcggaat ggggacattc cgattgacca tcctctcccg tcgcttgctt
    12301 ttggtcctct cctcaagcgc atgagtgcaa tcttgtaatg gggcgtgcgg aggtgcggcg
    12361 tggtttcaag gtccgggatg acgtagggga agggaagcat gccaaaagca aggatttact
    12421 gccccctcgc gacattctac cgccggctgg caatttcgag cgccgaaatt gcaaaaacac
    12481 tcaatgtcta cttctctcca agggtgagca ccacggcagc ctccgttgtc agcaaccgga
    12541 aggtaaaggt ctccagaaaa tacagttcga catctgtact ggaatgacct gagtaaccaa
    12601 tggagatgtc ttggccgata tccagtttaa aatcaccgcc gcgggtcgtg accacgaacc
    12661 cgccttcaat ggcgggcgcc cagacgaccc ctccgtccac cacgcgctca atatggttaa
    12721 agaccgggta accctcgtcg cttcctccac tcgcggcagt ataggcgtct gtcccgagca
    12781 caagcgcgta cggtccattg acgccggcaa gccgcaattg gctcacagcc tgcgccactg
    12841 catccggata gcccctcacg ctggaaggga gtacgacttc tgaattgctg ctgccttcac
    12901 gaatgccttg aattccagca gcggcgtatc cattgaagat agcgcgatct tccgcaaagg
    12961 cgattttccg tgccgcgtct ttcatcggcc gccagtccga atccgttgcg ccgcgctcca
    13021 cgtcgtcgat tgcctgccga gcgagttcga atggaacgcg tagttcgacg attgctttta
    13081 catcgcgttg caccgcctga atcccgtcgc cgggggcttg gatctggtga agatgcccgg
    13141 tgctgacagc cgagagctga aggccttttg gctccggaac gtctacgacc cgtcgtgccg
    13201 caaaatggcg cttcagagtg cgggccgtct cctcctcgat ctgagcccag gctttgccgg
    13261 aaataggcgc gagttcccga tggagattat tcactttcta cctctttttt cattttttca
    13321 gaggacggat acctggcgac cgatgacatg gccgctgagt cttcaaagtt tgaacacgaa
    13381 gtcatggaca cacacttcgg cgcggcattt tcgagaaggt cgcggatggc aggaaaaaca
    13441 atgtaccgta caggcggccg aagcccagtg aagattataa tttcccctcg tccaacttga
    13501 tgtgggaaag tttcatccgg cctataattc gctcttgttc gattgctgag cgagaaatca
    13561 gtagccgccc gaaccagatg gtgcccgagg cacgctagac ccttgcgaag agctcgagcc
    13621 cacgccagat cctggcattc tggaagaacc cgttgaggac ccttttttgc ttgccattcc
    13681 ctgtccggtc gaagattgtc cggacatgga tgactc
//
LOCUS       DASSHJ010000143         5090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1035066,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000143 DASSHJ010000000
VERSION     DASSHJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035066"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFI43_05685"
     CDS             <1..432
                     /locus_tag="VFI43_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7061651.1"
                     /translation="EDARAMAVYLKSLPESKPRTQSTPPLVTEQVRDWLEQGAKIYKK
                     HCEDCHGASGQGAPGIYPHLAENRGVMLDPPVNAIRIVLNGGYPPSTSGNPRPYGMPP
                     FAQLLNDGEVALVLSYVRNAWGNRASLVTPEQVDKSREGLH"
     gene            complement(790..1227)
                     /locus_tag="VFI43_05690"
     CDS             complement(790..1227)
                     /locus_tag="VFI43_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET7061652.1"
                     /translation="MESFLPKPAFSLAFIALIASLLVSSQNATAGKEIQVSLNGEQEV
                     PPVQTTASGNGTITVEDDKSIKGTITTSNIKGTGGHIHEGSAGKNGPDIITLTKTSDN
                     EWTIPSDAKLSDAQYDAFKAGQLYVNIHSDAHKSGEIRGQLKR"
     gene            complement(1495..1986)
                     /locus_tag="VFI43_05695"
     CDS             complement(1495..1986)
                     /locus_tag="VFI43_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HET7061653.1"
                     /translation="MFRWVSSRGLRAIAVVCVLWPAAARAELIGEVDTAFRWLGKDDR
                     VIVEAYDDPKVHGITCFVSRARTGGFKGTVGLAEDRSEASIACRQVTDSLQFTEKLPQ
                     QEDVFTERMSILFKRLHIVRIVDPRRNVLIYLTYSDRLIEGSPQNTVTAVPVPHSMVI
                     PLK"
     gene            2342..3532
                     /locus_tag="VFI43_05700"
     CDS             2342..3532
                     /locus_tag="VFI43_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7061654.1"
                     /translation="MNSGRDESQAEQRAADSERVVQEAIADGEKMPVETTSNPGVNIA
                     VWIIALIAVVAALYLGRAFFVPLLLGILVSYALRPIVDWFERYRIPRALGAALILVVL
                     VGGASWMTFSWSDEASAIIERLPDAARKLRQNLSLLRTEGSSAFRRVQEAADEIEGAA
                     VDAGLKSAAAPVVVTRESEANAWLRDFMLAQSALLVALIAQAPMVLLLTYFLLASGTH
                     FRRKLVQVVGSSLTRKKDIVRMLEEVDKQVQRYLLVMLTSNALVGILTWLAFEMLGVE
                     HAGVWGVAAGVLHFVPYLGTLVIALASGVSGLLQFDALPPALAVAGLSVLVSGTVGMV
                     FTTWLQGKFARVNAAVLFIVLLFFGWLWGIAGLLLGAPLLAIVKVVCDRVEPLKPLGE
                     LLGR"
     gene            complement(4528..>5090)
                     /locus_tag="VFI43_05705"
     CDS             complement(4528..>5090)
                     /locus_tag="VFI43_05705"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET7061655.1"
                     /translation="VAMMAPLLIPSIQYVRQRSFKHRRARAVALFVAGYGASWMAAGV
                     VVLAIEITVKPLAPMSFLPAAGAALIAVVWQFSPIKQQALNRCHSHTELAAFGAAADS
                     AVLRFGLTHGVWCVVSCWALMLLPMLLPSGRVAAMAVVAFLVFSERLDRPMPPSWRFR
                     GLTSVKRMVVARARIQFEGLRARSGA"
BASE COUNT         1131 a         1326 c         1408 g         1225 t
ORIGIN      
        1 gaagacgccc gggcaatggc cgtctatctg aaatcgttgc ccgaaagcaa gccccggacg
       61 cagtcgacac ctcccttagt gaccgaacag gttcgtgatt ggctggagca aggcgcaaag
      121 atctacaaga agcattgcga agattgccat ggggcgtcag ggcagggggc accgggtatc
      181 tatccgcatc tcgcggaaaa ccgcggcgtc atgcttgatc ctccagtgaa cgcaattcgc
      241 atcgtcctca acggaggata cccgccttcc acctcaggga accctcgtcc ctatggaatg
      301 ccgccattcg cgcagctctt gaacgatggc gaggtggcgc tcgtcctctc gtatgtccga
      361 aatgcatggg gaaaccgcgc cagtttggtg acgcccgagc aggtcgataa aagccgcgag
      421 ggccttcatt gacttggcca tgcgtgtcgg cagcgttacg ccggtgtatt atccctggag
      481 tctataaggt cgttgctggg ggctgggaca gtggaagcgg agacctcttc cccgggtttt
      541 ttagaattaa cgggagaggg agattgctgg actacgccgc tttcctcctg gttaaatcga
      601 tggcgagcgc caatatgccc gggtccgttc cgaaaatatt ttgtcatggc aagcggtttc
      661 cacgaatgag ttattgattg ttttagattg ccgccggaca cgtcccatca atgccagagg
      721 gatatgctcc ggactgctgt ggctattccc gtttgtcatg catctatccg atgaatcgtc
      781 acggctgcgt cagcgtttga gctgtccgcg aatttcgccg cttttatggg catcgctatg
      841 gatgttgacg tagagctggc ctgccttgaa ggcatcatac tgcgcatccg aaagtttcgc
      901 gtcgcttgga atcgtccatt cattatccga ggtcttggtg agcgtaatga tatcagggcc
      961 gtttttcccg gctgatccct catggatatg tccacccgtc cccttgatat tggaagtggt
     1021 tatcgtgcct ttgatcgact tgtcatcttc gaccgttatc gtgccgtttc cggaagcggt
     1081 cgtttgaaca ggaggaactt cctgctcgcc attcaggctg acttgaattt cttttccagc
     1141 cgttgcgttc tgcgagctaa ccagcaagct ggcgatcaac gcaataaagg cgaggctgaa
     1201 cgccggttta ggaagaaatg attccatttg agtggcctcc ttcgggatgg tgattaattg
     1261 ggatcagtgc ttagcgaggc gtgcccgtgt tcatatacgt tattcaccgg ggtcaacgcg
     1321 ccggacagta ctttaaataa acagccgaca atgagtccgt acggtagcgt acgtaaataa
     1381 atgattctca ttcttattga cagggaatat atatgctttg ccggtatgag gagaggagtc
     1441 tcgcgatgcg agactgcggg acgatgtgtg tttcccgagc gtttgtaacc ccttttattt
     1501 gagcggtata accatactgt gcggaacggg gactgcggtg acagtgtttt gggggcttcc
     1561 ttctatcagt cgatcagagt aggtcaggta aataaggaca ttgcgccgcg gatcgactat
     1621 tcggacgatg tgcaggcgct tgaagagtat ggacattcgt tccgtaaata cgtcctcctg
     1681 ttgcggaagc ttctctgtga actgaagcga gtcggttacc tgtctacatg caatcgaggc
     1741 ttccgatcga tcttcagcca gacctacggt acctttaaag ccccccgttc ttgcacggga
     1801 aacgaagcag gtgatgccgt gcacctttgg gtcatcgtag gcctcgacaa tgacccggtc
     1861 atcctttcca agccaacgga aagcggtgtc cacctcgccg atgagctccg ccctggccgc
     1921 agcgggccag agtacgcaga caacagcgat agcccgtaag cctcgagaac tcacccatcg
     1981 aaacatcttg gatctccgta cattgattgc attcgccgaa ctatcggaca ggtctttaaa
     2041 ggtgcatgtc cctaccttgc ccgctaaagc ttcggcactg cagaaagact gcggactgat
     2101 ccggggcaaa cggcggggag cagctttacg ttctcgacgc ccagtgttgg ggcgaggctc
     2161 aggattttaa cctaaaacca ggaccccgcg gcatattagc gaagctcgat ggatatcgaa
     2221 catcgggcgt acggctatct tctttcggag tatattcacg gagtatattc atatgggatt
     2281 tggcggagtt aatccgaaaa acggatgaac gtccagtctt tattctttca gataagcgag
     2341 catgaattca ggccgagatg aatcccaggc cgagcagagg gcggcggatt cggagcgtgt
     2401 tgtacaggag gcgattgccg acggcgaaaa aatgccggtg gaaacgacat ccaatccggg
     2461 tgtcaacatc gcggtctgga taattgcatt gatagctgta gttgccgcgt tatatctggg
     2521 gcgcgctttt ttcgttccat tattgcttgg catccttgtc agttatgcat tacgtccaat
     2581 agtggactgg ttcgagcgct atcgcattcc ccgcgcgttg ggagcggcac tcattctggt
     2641 agtgcttgtc gggggggcct cgtggatgac attttcctgg agcgatgagg cctctgcaat
     2701 cattgaaaga cttcccgatg ccgcgcgcaa actgcgacag aacctgagcc tcttgcgtac
     2761 cgaggggtca agtgcgtttc ggcgcgtaca ggaagccgcc gatgaaatcg agggagctgc
     2821 tgtggacgca ggtttgaaat ccgctgcagc gccggtggtt gttacgaggg aatccgaagc
     2881 gaatgcatgg ctgcgcgatt tcatgcttgc gcaatctgca ctgctggtcg cgcttattgc
     2941 acaagcgccc atggtattgc tgcttaccta ttttctgctc gcttccggta cgcatttccg
     3001 gcgcaaactg gtccaggttg tcgggtcttc gcttacccgg aagaaagata ttgtgaggat
     3061 gctcgaggag gtggataagc aagtccagcg ctacctgctt gtcatgctga catcaaacgc
     3121 cctggtcggg attcttacct ggcttgcgtt cgaaatgctt ggcgtcgagc atgcaggtgt
     3181 atggggagtg gctgccggcg tactgcattt tgttccttat ctggggaccc tggttatcgc
     3241 actcgcaagc ggtgtgtcag gcttgctgca atttgacgct ctcccgcccg cgctcgccgt
     3301 ggccggttta tccgtactcg tctcgggtac ggtggggatg gtgttcacga catggttaca
     3361 gggtaaattc gcgcgcgtga atgcggcagt tctattcatc gtacttctgt tttttggctg
     3421 gctgtggggc atagcgggat tgctgctcgg cgctccattg ctggctatcg tcaaagtggt
     3481 ttgcgatcgt gttgagcctc tcaaaccctt gggtgaactg ctcgggcgtt agaaaagcac
     3541 tccatcttca tagatcagtt tctatagaaa cacactgcga tagctcagcg tttccgaact
     3601 cttgacggtg gcgaaaccct tgaaattttc gtttacgccg ttgcgaaggc gagaaatagt
     3661 gtgatcgcga tcaatggata ttccatccga tatcctcctt gatctcgtca gcagcacgtg
     3721 agaccgcgga cgcactgtct tgccagaaaa acaatggcca tacggaaccg gctaaaccac
     3781 gtctgcttat atttctttcg ccttcaaggc aatgtcttgg gccgaatgag catttccctt
     3841 atataggact gaattctgat cagaaggaga tgttgggttc gcccataggc agggtcctaa
     3901 agaactcgat ttcagaagat tgagaagaag cagcaaacgg gcaagaagta cagcagtaac
     3961 atttttggtg ttgcctttgc caagccctca gcccacccac cgccccccat tgcttttgca
     4021 atacaagctc tcgtatcccc atgttataaa agcggttata taaaaagcat aataatgagg
     4081 gacatggcgt gaacgctttc aaagcgtgga ttttgtacgt gagctacgcc attgcgacta
     4141 tgctattcat catttaatcc aggttggaaa atcgaaattg gaaaaggagc aaagcacgat
     4201 gacttccctt cagaatccct ttccaaggta gaggccttct tatctgcgct ctaccagcag
     4261 aatttcttta aggacgagtc gacgcagggt agtgtcctct ggtacatcgc tttttacaaa
     4321 aaacgtgccc ctcagaggcg ccaggccttt cgagcttggg ggtttatatt gttattttta
     4381 agcattagtc tcccgttcct gacccagatg gtcgaaaaag agcaccaggc gcaggtggcc
     4441 tccacgcttg cttggctaat agctgcttcc cgaaacaaga cttgatgttt ccttcacaag
     4501 ttgtaaattc agatcgggaa acgccgttca ggcgcccgaa cgtgcccgca gaccctcgaa
     4561 ctggatgcgc gcgcgagcca ctaccatccg tttcacgctg gtcaatccgc gaaatcgcca
     4621 ggacgggggc atcggccggt ccaaccgctc actgaaaacc aggaaagcca ctacggccat
     4681 ggcagccacg cgccccgatg gcagcaacat aggtaacagc atcaacgccc agcaggaaac
     4741 cacgcaccag actccgtgcg tcaagccaaa acgcagcacg gcgctatcgg cggcggcacc
     4801 gaatgcggca agttcagtat gcgaatgaca gcgattgaga gcctgctgct tgatgggcga
     4861 gaactgccac accaccgcaa tcagcgccgc gcctgccgct ggcaaaaacg acatcggagc
     4921 cagcggtttg acggtaatct cgattgctag cactacgacc ccagcggcca tccaggacgc
     4981 tccataaccc gcaacgaaga gcgccacagc gcgcgcccgg cggtgcttga agctgcgttg
     5041 ccgcacgtat tgaatagaag gaatgagcag tggcgccatc attgccacca
//
LOCUS       DASSHJ010000144         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_382260, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000144 DASSHJ010000000
VERSION     DASSHJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382260"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFI43_05710"
     CDS             <1..234
                     /locus_tag="VFI43_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061656.1"
                     /translation="GGIVGTSGARTSGDINQQRYDAGYMQCMYAKGNRVPLAGRVMEN
                     SAAGAGTQGAGAPPPPPGTPPPSLETPSTVGGP"
     gene            complement(274..774)
                     /locus_tag="VFI43_05715"
     CDS             complement(274..774)
                     /locus_tag="VFI43_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061657.1"
                     /translation="MIPDSVDISSRVSSNLSALIDARMKDPDAEQPVTLAAVRAYLAG
                     LLPASLEETEKLHNFPIDTSLLEELDTLIEEYGGDVLADDFADNSASEALSRVIDAVI
                     NDGNNETPPTLGTVRDAIASGLSAKLVGDGAFDEDEDDTLLTETEALIQRFGEDAVAE
                     RFLRYE"
     gene            complement(792..1436)
                     /locus_tag="VFI43_05720"
     CDS             complement(792..1436)
                     /locus_tag="VFI43_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET7061658.1"
                     /translation="MIFANRDDAAKRLATALSEYRGRNPLVLAIPRGAVPMAKIIANE
                     LSGEFDVVLVRKLGAPGNPEFAIGSVDETGWVYVADYADRVGASKEYIKNEAARQLEV
                     IRRRRHQYTPVRPPIDPSGRIVIVVDDGLATGSTMISALHALRSKNPAELICAVPVAP
                     PDTVEKISTSADRVVCLSQPINFRAVAQFYEEFPQVTDEEVIDALSASGHSIKD"
     gene            1853..2413
                     /locus_tag="VFI43_05725"
     CDS             1853..2413
                     /locus_tag="VFI43_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET7061659.1"
                     /translation="MKFIFQITSRDISHSDALESHVRQKAEKLEAFYPHMMGCRVVIG
                     VPHKHKHQGRAFSVRLDITVPGKELVVNHEADEDVYVSLRDAFDAARRQLEDHARRQR
                     GDIKAHTPVLHGRVKRLIPGEDYGFIETPDGQELYFHRDNLANINFDKLSEGSEVQFV
                     EDVASEGLQAKRVSAGKHHSPGGEAK"
     gene            complement(2487..>2701)
                     /locus_tag="VFI43_05730"
     CDS             complement(2487..>2701)
                     /locus_tag="VFI43_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061660.1"
                     /translation="EDLNEVIGQCMRWIKCALDEDRARRLRSFHPRSFKQSTCSVARF
                     LPARPARSQYMTGLYRRLRNHARYSP"
BASE COUNT          591 a          750 c          741 g          619 t
ORIGIN      
        1 ggcggcattg taggcacaag tggagccagg acgtcgggcg acattaatca gcaacgttac
       61 gacgccggct atatgcagtg tatgtatgcc aagggtaacc gggttccgct tgcaggccgg
      121 gtcatggaaa actctgcggc gggtgcaggc actcaagggg cgggcgcgcc tcctccgccc
      181 ccgggaacac ctcccccttc gctagaaacc ccatccaccg ttggcgggcc ctgacgggaa
      241 cgtcagggct acgttctgct gttttagcgg gcgtcattca taccgcagga atcgctccgc
      301 aacggcgtcc tcgccaaacc gctggatcag tgcttcagtc tctgtcagca gcgtatcgtc
      361 ctcatcctca tcgaaagcgc catcgccgac gagttttgcc gacaaaccgg atgcaattgc
      421 atccctcacg gttccaagcg taggcggggt ttcgttattt ccatcgttta tcactgcgtc
      481 aataacacgg gaaagggctt cgcttgccga gttatccgca aaatcatcgg cgagcacatc
      541 tcccccatac tcctcgatga gcgtatcgag ttcctcgaga agtgaggtat cgatcggaaa
      601 attgtgcaat ttttccgttt cctccaatga ggccggcagc aaacctgcaa gataagcacg
      661 cacggcggct agtgtcacgg gttgctcggc atccggatct ttcatccggg cgtcgatcag
      721 ggccgacaaa ttggaactga cacgagagga gatatcaaca gaatccggga tcatggtttt
      781 actcctgtaa gctagtcctt gattgagtga ccggatgccg agagtgcgtc aatcacctct
      841 tcgtccgtca cttgtggaaa ttcttcgtag aactgggcga ccgcccgaaa attaatgggc
      901 tgcgacaggc aaacgacgcg gtcggcactg gtgcttattt tttccacagt atctggcggc
      961 gctaccggta cggcacaaat gagctcggcc gggtttttcg atctcagcgc atgcagcgcg
     1021 gaaatcatgg tcgagccagt ggccagtcca tcgtccacca cgatgactat ccgtccagaa
     1081 ggatcaatgg gtggacgtac tggggtgtat tgatggcggc gccggcgtat gacttcaagc
     1141 tgtcgggcag cttcgttttt tatatattcc ttgctcgctc cgacgcggtc ggcatagtca
     1201 gcaacatata cccacccggt ctcatcgact gacccaatcg caaattccgg attaccgggt
     1261 gctcccagtt ttcgtacaag caccacgtcg aattctccgc tcagttcgtt cgcaatgatt
     1321 ttggccatgg gcaccgcgcc ccgcggaatc gccagcacaa gggggttcct gccgcgatac
     1381 tccgagaggg cagtggcaag ccgctttgct gcatcatccc gatttgcaaa aatcatatgc
     1441 ctccaatgcg gtttgcatgt cttgaatgtt cttcgataca caggtttccc tgccggtggc
     1501 tttcaatggc atacttcgct gtgtaagttg ccgcgcaggc ttgctgtcct ggcaggcaga
     1561 tagaaggtct tctcgaagac catcccaata tctggtttgc gggtttgatg ggttgcatga
     1621 taacaacaac cgtaagccaa acgcccgtct cggctctttc agtctagtct attcttgaga
     1681 ttcatccagc taattcaatc agtcttgagt cccggttatc gctctcttta gctactatac
     1741 ttagaatggt taacaggatt tcatgcgaag aaaagaagat gcggtgcggg agcgctcgcc
     1801 cgttatagtt atccgtagac cactacggct catctcattt gaggacgcca atatgaaatt
     1861 catctttcag atcacatcgc gcgatatttc ccattctgat gccctggagt cgcatgtgcg
     1921 gcagaaagcg gagaagctcg aggcgtttta tccgcacatg atgggttgtc gtgtagtgat
     1981 tggagttccc cacaagcaca agcaccaggg gcgagccttc agcgttcgcc tcgacatcac
     2041 tgttcccggc aaggagctgg tcgtaaatca tgaagcagac gaagacgtgt acgtctccct
     2101 gcgggatgca ttcgatgctg cgagacggca actcgaggac catgcgcggc ggcagcgcgg
     2161 ggatatcaag gcacatactc cagtgctgca tggaagggtg aaacggctga ttccggggga
     2221 ggattatggt ttcattgaga cacccgacgg ccaggagctc tattttcatc gcgacaatct
     2281 cgccaacatc aactttgaca agctatccga aggcagcgag gtacagttcg tcgaggacgt
     2341 tgcaagcgag ggacttcagg cgaagcgcgt gagcgcgggc aagcaccatt cgcccggagg
     2401 ggaggcaaaa taaaattcga tacggcaggg caagtagccg tcactcatcg atgccctcgc
     2461 tactccaggc gtcttcctgt tggcaatcac gggctgtagc gcgcgtggtt gcgaagccgt
     2521 cgatatagtc ccgtcatgta ctgactcctc gctggcctcg ccggaaggaa gcgcgccacg
     2581 ctgcacgtac tctgcttgaa agatcgagga tggaaagacc gtagccttcg tgcccgatcc
     2641 tcatccaagg cgcacttgat ccagcgcatg cactgtccga tgacttcgtt cagatcttcc
     2701 a
//
LOCUS       DASSHJ010000145         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1224320,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000145 DASSHJ010000000
VERSION     DASSHJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224320"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /gene="glnE"
                     /locus_tag="VFI43_05735"
     CDS             complement(<1..1294)
                     /gene="glnE"
                     /locus_tag="VFI43_05735"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET7061661.1"
                     /translation="MNFRIAPEEIINSILPLSRYLAHLLESSPDLREELMQNLHRPLR
                     REEMRQSLSIDSSGADSQEGLQRVLRKLRRRVMLRVATRDLAGLANLPEVMATMTDLA
                     EVAIGFALENQKRALSASDRYGSPIGAECGTAQELIVIAMGKLGGCELNVSSDVDLIF
                     VYPEGGDTDGRRSVSNQDFFSRLGRRVIAALQEITEDGYVFRVDMRLRPYGESGPLAM
                     SFAMLEDYFVTQGREWERYAWIKSRAITGSDRNVSALTEIARPFVYRKYLDFGAYESM
                     RDLHSQIRQEVQRRELHENIKLGPGGIREIEFISQVFQLIRGGRDPELRLRPTLAALQ
                     RLGEKQQLPERIIADLTGAYCFFRKLEHRLQYLDDQQTHTLPENAVDQAIVGTAMGFS
                     SYSDFLKKLEGHRDNVTRHFEKVFAKPRKSSTLLTLPRG"
     gene            1602..>4653
                     /locus_tag="VFI43_05740"
     CDS             1602..>4653
                     /locus_tag="VFI43_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HET7061662.1"
                     /translation="MKLSIKPDLRPGNSFLLRGFAAALVVVAACFALLLLSLRYWVLP
                     DIERYRENIAQAISRASGQVVTIGSIRAEWDGLHPHMMLGMIRVHDHKGNTTLLLHKL
                     EGSLSWLSILHGNFHFRDIRIDQPDLIVRRDSKGIIHVAGFALSDAAEENESGFSDWL
                     LQQEHVTIRNASISWQDDQRSAPELELLVNLRLENRGDHHRFGILAVPPAELARRLDV
                     RGDLTGKSLGTPERWRGRLFVRIDQGDIASWRPWLPLPGQVKLDQGAGGLRMWAEIDG
                     TDIKKLSADMRLRNVRVQLGDDLPQLDLLRLEGRAGWQKVHDGNREGDRWYVRQLYYA
                     LRNKQSGEPVNFSLQLMQRHGDKPGGGKMSASALNLEMLTDLMDYLPVSQTLGQEVSK
                     ASARGDLRSLSVNWSGEWIAPVSFSVRGSFDNVSMSQVGKIPSFSGMSGNIDATEKRG
                     SLNLRSRDAKLELPDLFPEPLIADMLIGHVNWSSADRNSTMLKLSNVSFSNHFASGSA
                     SGTYHLKSDGVDTIDMVGRLANVDARYLARFIPDTTGPSDSSLANSVVAGKFSDVRVH
                     AKGPLENFPSRKQDWGILRAQARASGVTLDNLRGWPRIENISGSLLLQQNRIEFDSGQ
                     ASILGTRVTRGYLHIPDITSTDAILLGQIEASGPTQQFFNIATRYSGEAFKAPLLDSA
                     KFTGEGRLWLTFSSPLSSGSTKLSGNYQFIDNQLYPGSRIPGLKSINGMLGFNNSGIK
                     IQNVIARFLGGPVFINAGNMQDGSFHLAASGTLNPGNVNEITDGKMAEAVPLLVKHLH
                     GSTGWRASMQVSDKATSLIVESSLEGLSSNFPAPFAKSSVEEVPFRFERISTGPDKDE
                     ISITYGRRAAARINRIRKESGEYRVDRGVVAFGALLPATAAKAGVTVMGTLPLLDADQ
                     WLGQIKQFKLQSNMSTEASAIMSPVNIQAVNIQLGVLNFLGRQLTDVTLSASKEDGVW
                     RSTVTGKEISGNVSWDPSGRGKIAARLSKATVSPASSTSNVPPRY"
BASE COUNT         1105 a         1267 c         1232 g         1049 t
ORIGIN      
        1 cccccctcgg caaggttaac aaagtcgaag acttcctcgg cttggcgaac accttctcaa
       61 aatgccgggt aacattatcc cggtggccct caagcttttt caaaaaatcg ctgtagctgg
      121 aaaaacccat tgccgtcccc acgattgcct gatccacggc attttcaggc agggtatggg
      181 tttgctgatc gtcgagatac tgcaagcgat gctcgagttt tcgaaaaaag caatatgcgc
      241 cggtcaggtc agcgatgatc ctttccggta attgttgctt ttcaccaagg cgctgcagcg
      301 ctgccaaagt cggccgaagg cgcagctccg ggtcgcgccc gccccgaata agctggaata
      361 cttgcgagat aaattcgatt tcgcgtattc ctcctggtcc cagcttgatg ttctcatgca
      421 attcccggcg ttgaacctcc tgccgaattt gagagtgaag atcgcgcatg gactcatagg
      481 caccgaaatc caggtatttc cggtagacga atggtcgagc aatctcagtc agcgctgaca
      541 cgtttcgatc gcttccggtt atggcacgac tctttatcca ggcatagcgc tcccactcgc
      601 ggccttgcgt gacgaagtaa tcctcgagca ttgcgaaact catggcgaga ggcccgcttt
      661 ctccgtacgg gcgcaagcgc atatccaccc gaaaaacata tccgtcttca gtaatctcct
      721 gcaaagccgc aatgaccctt ctccccagac gggaaaaaaa gtcctgattg ctgactgatc
      781 ggcgtccatc cgtatccccg ccctcggggt aaacgaaaat cagatcgacg tccgatgaaa
      841 cattaagctc acatcctccc agtttgccca tcgcaatcac gatcaattct tgagccgttc
      901 cgcattcggc accgatgggt gagccgtagc gatccgatgc cgaaagcgct cttttttgat
      961 tttcaagagc aaaaccaata gctacttccg ccaagtcggt catggtcgcc atgacttctg
     1021 gcaggttcgc cagtcccgcc agatctcttg tcgccacacg caacatcacg cgccttcgca
     1081 atttgcgcaa aaccctttgc agaccttctt ggctgtctgc cccagaggaa tctatactga
     1141 gagattggcg catttcctcc cttctcaggg ggcgatgcaa attctgcatc agttcctccc
     1201 gaagatcggg gctgctctcc agtaaatgcg ccaggtaacg gctgagagga agaatggaat
     1261 tgatgatttc ttctggcgca atacgaaaat tcatggttaa cgaaaattca tggttaagga
     1321 ttccgagttg gaacacgcac tccggattat tctttttgca tacccgaaga aacctcctta
     1381 agccgccctg cacgggattt atgaatcgag tccgtctagg gcgattgaac gaactgcggc
     1441 aggtcttatc taaaaacgtt tttgcgcgcg ttcccctgta ggggtccccg tggggaacac
     1501 gtgtccttcc aagctttgac cctacctgtt cgattatttg cgcttcttct cctgcatcga
     1561 ctccaagaga tggccggtaa acatacaatc ggaacttcgc attgaagtta tcgataaaac
     1621 ccgatctccg acccggaaac agctttctgc tcagagggtt tgcagcagcg ctcgttgtcg
     1681 ttgcggcgtg ctttgcttta ttgctgttat cgctccgcta ctgggttctg ccggatatcg
     1741 agcgctatcg ggaaaatatc gctcaggcca tcagtcgagc ttccggtcaa gtcgtcacga
     1801 taggctcgat cagggccgag tgggacggcc ttcatcccca tatgatgctg ggcatgattc
     1861 gggtacacga tcataaaggc aacacgacgc tgctgttgca caagctcgaa ggctcgcttt
     1921 cctggctttc aatattacac ggtaattttc atttccgtga catccgcatc gaccagcccg
     1981 acctgatcgt gcgcagggat tccaaaggaa taatccatgt ggcgggtttt gcgctgagcg
     2041 acgccgccga agagaatgag agtggctttt ccgattggtt gttgcaacag gaacacgtga
     2101 ccatcagaaa cgccagcata tcgtggcagg atgatcagcg gagtgcccct gaactggagt
     2161 tgttggtcaa cctgcgcctt gaaaatcggg gtgatcacca tcgctttggt atcttggcgg
     2221 ttccacctgc ggagctcgca agacgactgg atgtcagggg agacctgacc ggaaaatctc
     2281 ttggaactcc agaacgatgg cgtggacgac tgtttgtaag aatcgaccag ggtgatatcg
     2341 catcctggcg cccgtggctc ccccttccag ggcaagtaaa gctggatcag ggagccggcg
     2401 gacttcgaat gtgggctgaa atagacggca cagacataaa gaaattatcg gcggacatgc
     2461 gtttacgaaa tgtacgcgtc caactgggcg acgacttgcc gcaactcgat ctgctccgtt
     2521 tagagggtag agcgggatgg caaaaggtgc acgacggaaa ccgggagggc gacagatggt
     2581 atgtccggca attgtattat gctttgcgta acaagcagtc gggggagccc gtcaacttct
     2641 cactgcagct catgcagcgc cacggggaca aacctggcgg cggaaaaatg agcgcgagcg
     2701 cactcaacct tgagatgctc accgacctta tggactatct gccggttagc caaacattgg
     2761 gtcaggaggt aagcaaagct tccgcgcgag gggatctacg atctttatcg gtgaactgga
     2821 gcggagaatg gatagcgccg gtcagcttta gcgtaagagg tagcttcgac aacgtcagca
     2881 tgagccaggt aggtaaaatt ccctcgttca gtggcatgag tggaaacatt gatgccacag
     2941 aaaaacgggg gtcgctaaac cttcgctccc gcgacgcaaa attggaactg cccgaccttt
     3001 tcccggagcc tctgatcgct gatatgctga tcggccatgt aaactggagt tcagcggaca
     3061 gaaattcgac aatgctcaaa ttgagcaatg tttccttttc caaccatttt gcctcgggat
     3121 cagcttccgg gacttaccat ctcaagtccg atggggtaga caccatcgat atggtcggca
     3181 ggcttgcaaa tgtcgacgcg cgctacctcg cgcgctttat accggacacg accgggccgt
     3241 ccgatagctc cctggcaaac tcggtggttg cggggaaatt ctccgatgta cgcgttcacg
     3301 ccaaggggcc tctcgaaaac ttcccttcca ggaaacaaga ctgggggatc ctccgggcac
     3361 aagcgcgagc atctggcgtg acgcttgata accttcgggg gtggccaaga atcgagaaca
     3421 tttcgggaag tctcctcttg cagcagaacc gcatcgaatt tgattctgga caagcttcta
     3481 tcttagggac ccgggtgacc agagggtatc tccacattcc cgacatcacc tccacggacg
     3541 ctatcctcct tgggcagatc gaggcgagcg ggcctacgca acaattcttc aatatcgcca
     3601 cccggtattc aggggaggcc ttcaaggcgc ccctccttga cagtgcgaag tttactgggg
     3661 agggccgatt gtggttgacc ttcagtagcc ctctgtccag cggttccacc aaattgtcgg
     3721 gaaattatca attcatagat aaccaactct atcctggttc ccgcattcca ggcctgaaga
     3781 gcatcaatgg catgctcggc tttaataatt cagggatcaa aatccagaac gtgattgccc
     3841 ggttcctggg cggaccagta ttcatcaacg ccggcaatat gcaggatggc agttttcacc
     3901 ttgccgcttc aggtacgctc aatccaggga atgtaaatga gatcacagac ggaaaaatgg
     3961 cagaagccgt cccgctgctg gtcaagcatc tgcacggcag tactggttgg cgagcctcca
     4021 tgcaggtgag cgacaaggca accagtctga ttgtcgaatc gtcactcgaa ggcctctcct
     4081 cgaatttccc cgcaccattt gccaaatctt ccgttgagga agtacctttc aggttcgaaa
     4141 ggatatcgac ggggccggac aaggatgaaa taagcatcac ctatggccgc cgggctgcgg
     4201 cgaggataaa ccgcattcgc aaggagtcag gggaataccg ggtagatcgg ggcgttgttg
     4261 ctttcggtgc cttactcccg gcaacagccg cgaaggcagg cgtaacagtc atgggaacat
     4321 tgcccttact ggacgcggat caatggctcg ggcagatcaa gcaattcaaa ctgcaatcaa
     4381 acatgagcac cgaggcttcg gcgatcatgt ccccagtcaa tatacaagca gtcaacatac
     4441 agcttggggt acttaacttt ctaggacggc aacttacaga tgtcacgttg agcgcaagca
     4501 aggaagacgg cgtatggcgt tccacggtaa caggcaagga aatcagcggc aacgtcagct
     4561 gggacccttc gggcagggga aaaattgccg cgcgactgag caaggccact gtttcacccg
     4621 cttcctcgac ttcaaatgtc ccgccccgtt atc
//
LOCUS       DASSHJ010000146         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_255808, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000146 DASSHJ010000000
VERSION     DASSHJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255808"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..815
                     /locus_tag="VFI43_05745"
     CDS             141..815
                     /locus_tag="VFI43_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7061663.1"
                     /translation="MSTSSNIPTRRARVMLVDDHAMLRHGMVMLINLEPDMEVVAEAG
                     DGDETLSILKNSSPPDIVLMDLSLKTISGFEVIKSVHSLIPGLPVLVVSMHDEAVYAQ
                     RALRAGARGYVMKHEPGEVLVTAIREVLKGNIYLSKQMHARLLNQVAAGGAKPEPLIN
                     SLTPSEFEVLHLIGSGHSSQEIAKLLHRSIKTIETHRFNIRSKLNLKDGADLIRYAAQ
                     WISEQS"
     gene            1026..1460
                     /locus_tag="VFI43_05750"
     CDS             1026..1460
                     /locus_tag="VFI43_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061664.1"
                     /translation="MKSTTERLVAVLSAVTLLASCAETAPLERKNANSHVASEKAKRP
                     ADHDRLARQYENAAKQLQAKAEEQKKLLRHYEEKSYLYGRQAQDNQSHAAAMLSRYQH
                     DAEETMELAAFHHRMASELAKREHAGQIAEKPGSKQTGETGA"
     gene            2199..>2824
                     /locus_tag="VFI43_05755"
     CDS             2199..>2824
                     /locus_tag="VFI43_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061665.1"
                     /translation="MRSKGESRSGFVKSSLITFLLAAVAVPALAESNVEKLRNMFRSV
                     NSHGIDATFSTAGYIDLDNPFFKSLGTNGRSCSTCHVPSEGWVITPKGVQARFEKTKG
                     MDPLFRLNDGANSPAARVSTLEERRNAYSMLLSKANIRVGVGVPPNAEFTLVEADDPY
                     GHASATELSLFRRPMPSTNLKFLSSVMWDTRETTQMPGSGNCIFGTTT"
BASE COUNT          755 a          690 c          723 g          656 t
ORIGIN      
        1 cactcacccc tggaatgggt atcaaaatta tgcggcatcg cgcaaggcaa ctcggagcaa
       61 ctcttgaatt cacgggcccc aaagaggggg gcatggaagt gcgactggaa atgagaatgg
      121 tttaggcgga ccatccaacg atgtcaacct cctccaacat tccgacccga agagcacgag
      181 tgatgcttgt ggatgaccat gcaatgctgc gtcacggcat ggtcatgctg atcaacctcg
      241 agcctgatat ggaggtcgtg gcggaggccg gagacggtga cgaaacactg tcgatactca
      301 agaacagctc tcctcccgac atcgttctga tggatttgtc tctcaaaacg atttccgggt
      361 tcgaggtgat taaaagcgtg catagcctga tacccggatt acccgtgctc gttgtatcaa
      421 tgcatgacga ggcggtatat gctcagcgcg cacttcgggc tggtgcacgt gggtatgtaa
      481 tgaagcacga gccgggagaa gtcctggtga cggcgattcg cgaagtactc aaggggaata
      541 tttacttgag caagcaaatg catgcgcggc tcctgaacca ggttgctgcc ggcggcgcaa
      601 aacctgaacc cttgatcaat agtttgactc caagtgaatt tgaagtactg catctgatag
      661 gttcgggtca tagtagccag gaaatcgcca aactgcttca ccgcagtatc aaaacgatag
      721 aaacccaccg attcaacatt cgctcaaaac tgaacttgaa agacggcgcc gacctgatac
      781 gttacgccgc ccagtggatt tcagagcaat cctgaaaacg ggtgcagatg ggaatgagta
      841 aatgggaatg agtatgagag gcagcgctaa tttggccgcg aggataagtt gtaaagccac
      901 ttctgccaca aaaatattac ctaagggttt cccttagaat aaaacaaggg tttccccaat
      961 atacggatag tattgactcg gataggattc atcctactta attctattcg ggttcattac
     1021 catccatgaa atccacaacg gaacggctcg tagcggtttt atcggcggtc accctgctgg
     1081 cttcttgcgc ggaaacggct cctctcgaaa ggaagaacgc aaatagtcat gtggcttcag
     1141 agaaagccaa gaggccggct gatcatgatc gtttggcgag gcaatacgaa aacgccgcca
     1201 agcaattgca ggcgaaggca gaggaacaaa agaagctgct tcggcattat gaagaaaaga
     1261 gctatcttta tggtagacag gcgcaagata accaatcaca cgctgctgcc atgctcagca
     1321 ggtatcagca tgacgcggag gaaaccatgg agttggccgc ctttcaccat agaatggcgt
     1381 ccgaacttgc aaagcgtgaa cacgccgggc aaatagcgga aaaaccaggg tcgaagcaaa
     1441 ccggggaaac tggggcctga gccagcaagc ttatccctac tgaccgagtt tcagaaactg
     1501 gcgacgcccg gttgtagtag ttcgatttaa tctggcttta atctgacagt gggccccgag
     1561 attattcggg gtcttagttt tcgttcttct ttcgcgctta tgactgtgta cgctatggaa
     1621 tcaggtgtac tcgctcgagg cgtgcccttc cgaattttcc tcgatagctc tacaacgggc
     1681 catcagtaaa tatcccacat catatcatcg ttgttcgcat cgagatgccc tcacttgagt
     1741 gtgttcgcgt ctgccgccta aaaaacagat ttgtgatcta agacactcgc cagctgcggt
     1801 ttacccggct ggttctctac ttcctttcaa agacaaaccc tcgtcataag cgaagcccaa
     1861 accggactat atccgttccc taaaaatgga tataataagc cccacatttt cagggacttg
     1921 ctctagctct accattattt ccgcagaaat caatattctt ggcatagcat tgctgccgca
     1981 ctgcctgttg tacagatcat cccaaagcta cagcttgagc atcacctcat ccatgagcaa
     2041 ttcgtaattt tcctatctca caaacggcgc gagcaagaaa aaaagaagaa gcaatacgcc
     2101 ttctcagcgc cgcttgctga aaatgccagt tttaaatcgt tgctgacaat tatctgcccg
     2161 ggggggggat tattttttaa cacaaccgtg gaggtaatat gcgtagtaaa ggggagagcc
     2221 ggtcgggctt tgtcaaatcg agcctgataa catttctgct tgcggcagtg gcagtgcccg
     2281 cgcttgcgga aagcaatgtt gagaagttgc gcaatatgtt caggtctgta aacagtcatg
     2341 gcatagacgc aacctttagc acagccggct atatagatct tgataatcca tttttcaaaa
     2401 gcctggggac gaatggccgt agctgtagca cgtgtcatgt accttccgag ggatgggtga
     2461 ttacaccaaa aggagtccag gcgcgctttg aaaaaaccaa aggtatggat ccgctgttcc
     2521 gcttgaatga cggcgctaat tcacccgctg cgagggtatc aacgctcgag gaacgccgta
     2581 atgcctatag catgttgctc agcaaggcaa acatacgtgt aggtgtcggc gttcctccca
     2641 atgcggaatt cacattggtt gaagcggatg atccctatgg acacgcgagc gcgacagagt
     2701 tgtcgttatt ccgccgtcca atgccctcga cgaatctgaa gttcctgagc agtgtaatgt
     2761 gggacacgcg tgaaacaacg caaatgccag gttcaggtaa ttgtattttt ggtaccacga
     2821 cctg
//
LOCUS       DASSHJ010000147         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_471255, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000147 DASSHJ010000000
VERSION     DASSHJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471255"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1199
                     /locus_tag="VFI43_05760"
     CDS             87..1199
                     /locus_tag="VFI43_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET7061666.1"
                     /translation="MKTRAAVAWGPAQPLAVEEVDLQLPKKGEVLVRIVATGVCHTDA
                     FTLSGKDPEGKFPAILGHEGGGIVEALGESVTSVSVGDHVIPLYTPECGQCEFCRSGK
                     TNLCQAIRATQGQGLMPDGTTRFSKDGKPIYHYMGTSTFSEYTVIPEIALAKINRDAP
                     LGKVCLLGCGVTTGLGAVANTAKVKAGDTVAVFGLGGIGLAVIIGAALAKAGRIIAID
                     TNPGKFGIARQLGATDTVNPKEYDKPIQDVIIEMTAGGVDFSFECIGNVQVMRSALEC
                     CHKGWGESVIIGVAGAGEEICTRPFQLVTGRVWRGSAFGGVKGRTELPGYVQRYLDGD
                     FELDTFITHTMGLEDINKAFDLMHEGESIRTVIHFN"
     gene            1281..2385
                     /gene="prfB"
                     /locus_tag="VFI43_05765"
     CDS             join(1281..1361,1363..2385)
                     /gene="prfB"
                     /locus_tag="VFI43_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239796700.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7061667.1"
                     /translation="MEAEQINAIEYKVTDLDTRAAELRRYLDFDAKQTRLSELTRLTE
                     DPGIWDDNKRAQELGREKKALEDVVIGLRTVTRQLNDARDLFEMAKEEDDDPALLSIQ
                     RDLANVEKTVADMEFRRMFSNPMDPNNCFVDIQSGSGGTEAQDWAAMLERMYLHYCER
                     RGYSVEVLEESPGEVAGIKSASLKVSGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTS
                     FASVFVYPEADETIEIDINPADLRVDTFRASGAGGQHINKTDSAVRITHNPTGIVVQC
                     QTGRSQHRNRADAMAMLKSRLYEAELRKRNEEKQAVEDSKTDIGWGHQIRSYVLDQSR
                     IKDLRTGIETGNTQAILDGDLDGFIEASLKQAV"
     gene            2390..>3758
                     /gene="lysS"
                     /locus_tag="VFI43_05770"
     CDS             2390..>3758
                     /gene="lysS"
                     /locus_tag="VFI43_05770"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7061668.1"
                     /translation="MVDHNNSEKSSQEKNLQDPSSQEPADRKGSELKLVEERRAKLAA
                     LREAGNPFPNDFRRTDLAAELHHSYAGNSNESLEAEPRMVSVAGRMMLKRVMGKASFA
                     TIQDMSGRIQLYIARDVAGEEAYSAFKHYDLGDILGAAGTLFRTKTGELSVRVTHLRL
                     LTKTLRPLPEKFHGLTDQEQKYRQRYLDLVTSEDSRKVFITRSRIIQAIRECLAARGY
                     LEVETPMMHPIPGGAAARPFVTHHNALDTDLYLRIAPELYLKRLVIGGMERVFEINRS
                     FRNEGISTRHNPEFTMLEFYEAYQDYAYLMKLTEIMLREISEKVLGTAKITYQGREID
                     LAQPFERLTITRAIEKFHPEYSEAQLGDHAFLISRLKALGVPFKPEDGIGGLQLTLFD
                     ETTEHLLFEPTFIIDYPAEVSPLARRNDSNPAVTDRFELYIAGREIANGFSELNDPDD
                     QKARFQ"
BASE COUNT          910 a          985 c         1095 g          768 t
ORIGIN      
        1 cctatgagac cataattgag gatggggcct gctctcatgc gacagggaca ttgagcaaca
       61 tgaattggag cgtttagatg gaaacagtga aaacccgggc agccgtggcg tggggtccgg
      121 cccagccttt agcggtggaa gaggttgatt tacagcttcc gaagaaaggt gaagtgctgg
      181 tgcgtatcgt cgcaacggga gtctgccata ccgatgcgtt taccctttcc ggcaaggacc
      241 cggaaggcaa atttcccgca atccttgggc atgaaggggg tggtatcgtt gaggcgttgg
      301 gggaaagcgt tacgagcgtc agcgtagggg atcatgtgat tccgctttat acccctgaat
      361 gcgggcagtg cgaattctgc agatcgggga aaacgaatct ctgccaggcg atccgggcga
      421 cccaggggca ggggctgatg cctgatggca ccacccgctt ttccaaggat ggcaagccca
      481 tttaccacta catgggcacg tcaactttct cggaatacac tgtgattccg gaaatcgcgc
      541 tcgcaaaaat caacagggac gcgccactgg gaaaagtgtg cctgctcgga tgcggcgtta
      601 caaccggact cggggccgtc gccaatacgg caaaagtaaa ggctggcgat actgttgcag
      661 ttttcggctt gggcggcatc gggcttgccg tcattatcgg cgcagccttg gcaaaagcgg
      721 gacgaatcat cgcaatcgat accaatcccg gaaaattcgg gattgcacgt caactgggcg
      781 caaccgatac cgtcaatcca aaagaatatg ataaacctat ccaggatgtc attattgaaa
      841 tgacagcggg cggggtggat ttttccttcg aatgcatcgg taatgtgcag gtcatgcgct
      901 cggccctcga gtgctgccat aaaggttggg gagagtcagt gatcataggg gtggccgggg
      961 cgggcgagga aatctgtacc cgtccctttc agctggtaac agggcgagta tggcgcgggt
     1021 ctgcgtttgg cggggtaaaa gggcgtacgg agttgccggg atacgtgcag cgttacctgg
     1081 acggcgattt cgagctcgat acatttatca cgcataccat gggacttgag gatatcaata
     1141 aagcgttcga cctgatgcat gagggagaat ccattcgtac agtgatacat ttcaactgaa
     1201 attcgacggt ctgccaagag cgttggagaa aattccgctt ccctttctgt ataatcgatc
     1261 cctttttcgg aataacgtcc atggaagcgg aacagattaa cgctatcgaa tataaggtta
     1321 cggacctgga tacccgtgcg gccgaattac ggaggtatct ttgacttcga tgcgaagcag
     1381 acccggctga gtgaactcac ccgtctgaca gaagatcccg gcatctggga cgacaacaag
     1441 cgtgcccagg agctgggcag ggaaaaaaaa gccctggagg acgttgtcat cggcctgcgc
     1501 accgtcaccc gacaattgaa tgacgcgcgt gatttgttcg agatggcaaa ggaagaggat
     1561 gacgaccctg ccctcctcag catccagcgc gatctcgcga atgtggaaaa aaccgtggcg
     1621 gacatggagt ttcgccgcat gttttccaac cccatggacc ccaataattg ttttgtggac
     1681 atacaatccg gctcgggcgg cacggaagcc caggactggg cggcgatgct ggagcggatg
     1741 tacctgcact actgcgagcg acggggctac tcggtggaag tacttgaaga gtccccgggc
     1801 gaggtggccg ggatcaagag cgcaagcctg aaagtatcag gcgagtatgc gtacgggtac
     1861 cttcgcaccg agacaggcgt gcaccggctg gtgcggaagt cgccgtttga ctcgggcaac
     1921 cggcggcata cctcgtttgc cagcgttttt gtttatcccg aagcagatga aaccatcgag
     1981 atcgatatca acccggctga cttgcgggtg gatactttcc gcgcgtccgg cgcgggtggc
     2041 cagcatatca acaagacgga ctcagccgtt cgaatcactc acaatcccac gggcattgtc
     2101 gttcaatgcc agacaggccg atcacagcac aggaaccggg cggatgcgat ggcgatgctg
     2161 aaatcccgtc tttacgaagc cgaactgcgc aaacgcaacg aggaaaagca ggcggtagag
     2221 gacagcaaga cggatatcgg ctggggtcat cagattcgtt cctacgtcct ggaccagtcc
     2281 cgtatcaagg atttgcgaac aggcattgaa acaggaaata cgcaagctat cctcgacggc
     2341 gatctggatg ggttcatcga ggcgagcctg aaacaggccg tataggagca tggtggatca
     2401 taataattca gaaaaaagtt cgcaggaaaa aaatttgcag gacccgagct cacaagaacc
     2461 tgctgaccgt aaaggctccg agctgaaact ggtcgaagag cgccgggcca agctggccgc
     2521 cctccgtgag gccgggaatc ccttcccgaa tgattttcgc cgaaccgacc tggcggcgga
     2581 actgcatcat agctatgcag gcaattccaa cgagagtctg gaagccgagc cgcggatggt
     2641 cagcgtggca gggcgtatga tgctcaaacg cgtgatgggc aaggcgagct ttgctaccat
     2701 ccaggatatg agcgggcgta tccagctata tatcgcccga gacgttgccg gcgaagaggc
     2761 ttattccgca ttcaagcatt acgatctggg tgacattctc ggggcggcgg gcacgctgtt
     2821 caggaccaaa actggcgaac tatcggtgcg cgtcacacat ttacgcctgc tcaccaaaac
     2881 gctgcgacct cttcccgaaa aattccatgg tctgaccgac caggagcaga aataccgcca
     2941 gcgctatctc gatctcgtca ccagtgaaga tagtcgaaag gtattcatta cgcgttcacg
     3001 gatcatccag gccatccgcg agtgcctggc cgcgcgcggc tacctcgaag tcgaaacccc
     3061 gatgatgcat cccatccccg ggggggcagc ggcgaggcct ttcgtcacgc accacaatgc
     3121 cctcgatacg gatttgtatc ttcgcatcgc accggagctt tacctcaagc gcctggtgat
     3181 cggcggaatg gagcgggtgt tcgaaatcaa ccgaagcttc cggaacgagg gaatttccac
     3241 tcggcataat ccggaattca ccatgctgga gttctatgag gcctaccagg attacgcgta
     3301 tttgatgaag ctgaccgaga tcatgctgcg cgaaatctcg gaaaaggttt taggtaccgc
     3361 caaaataacc taccaggggc gcgaaatcga tttggcccaa ccgttcgaac ggcttaccat
     3421 cacccgggct attgagaaat tccacccgga atattcggaa gcacagttag gcgaccatgc
     3481 ttttcttatc tctcgactga aggcgcttgg cgtgccgttc aaacctgaag acggaattgg
     3541 cggattgcaa ctcacgttgt ttgatgaaac caccgaacat ctgttgtttg agcctacgtt
     3601 catcatcgat tatcccgctg aagtctcccc ccttgcacga cggaacgact ccaacccggc
     3661 cgtcacggac cgcttcgagc tttacattgc cgggcgcgaa atcgccaatg gcttttccga
     3721 actgaatgat cctgatgatc agaaggcgcg cttccagg
//
LOCUS       DASSHJ010000148         8800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1092297,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000148 DASSHJ010000000
VERSION     DASSHJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092297"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1062)
                     /locus_tag="VFI43_05775"
     CDS             complement(145..1062)
                     /locus_tag="VFI43_05775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7061669.1"
                     /translation="MNIILISNNLAKTRSLTLTSAHVVLVAVVFFLTAVLLAMGLNYL
                     SLRYADKIDSPTLRSLILSVQQEEHQKAQSYLRDSLNAMAVRMGQMQAQLLRLDSVGA
                     RLAELSGIKPQEFLFNQAPGQGGVVPTLPSQEMSFIEFNNKIDELSRLLEDRSDKLGA
                     LDSLLMQDRLKKKMLPSVMPVNAKWYSSGFGVRIDPFSGRSAFHEGVDFTAAAGTPIV
                     AAAGGVVVYSDYHPEYGNMIDVDHGNDLVSRYAHASKRLVKVGQVVVRGQKIAEVGST
                     GRSTGPHLHFEVRHRGLPQNPSRFLKMPG"
     gene            1089..1553
                     /locus_tag="VFI43_05780"
     CDS             1089..1553
                     /locus_tag="VFI43_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET7061670.1"
                     /translation="MPPHKINFYLRLLSQGADHQRLFSHMTALTAMQHVFCGIAPPRL
                     ARHCALGGLSNGKLIIFARNSAIAAKLRQTTPSLLLKFQAKGYEVTAIRIAVQANSGT
                     HGNNTPATKRSIGGVGRESLRQLAVKLPQSPLKASVESLLHKASTAGRKTGG"
     gene            complement(1628..2794)
                     /gene="ftsZ"
                     /locus_tag="VFI43_05785"
     CDS             complement(1628..2794)
                     /gene="ftsZ"
                     /locus_tag="VFI43_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7061671.1"
                     /translation="MFEIMDTQTQEAVIKVIGIGGCGGNAVDHMILNGVQGVEFICAN
                     TDAQALKRNQARTLLQLGSAITKGLGAGADPEIGRNAALEDRERIAELIEGADMLFLT
                     AGMGGGTGTGAAPVIAQVARELGILTVAVVTKPFSFEGRRIKAAQAGMEALSQYVDSL
                     IVIPNDKLMAVLGEDVSMLDAFKAANNVLYGAVAGIAEVINCPGLVNVDFADVKTVMS
                     EMGMAMMGSAFAGGADRARVAAEQAVASPLLEDVNLSGARGVLVNITASAGMKMKEIH
                     EVMDVIRNFTAEDATVIVGTVIDEGMQEDLRVTMVATGLGGVANRALSKPFSIVHGRT
                     GTDNALFAEGVNWKEMDAPAAIRTNRRRDATVEAMKQSGVDMLDIPAFLRKQAD"
     gene            complement(2886..4124)
                     /gene="ftsA"
                     /locus_tag="VFI43_05790"
     CDS             complement(2886..4124)
                     /gene="ftsA"
                     /locus_tag="VFI43_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7061672.1"
                     /translation="MSRGNEAKNLIVGLDIGTSKIIAIVAEIKPEGGFDVIGMGTHPS
                     RGLKKGVVVNIDTTVNAIQRALEEAELMADCKIREVYTGIAGSHIKSFNSHGMVAIKD
                     KEVSRLDVERVMETAKAVNILNDQQVLHVLNQEFIVDGQEDVREPIGMSGIRLEVKVH
                     IVTGAVAAAQNIMKCVRRCGLEVRDLVLQPLASSLAVLSEDEKELGVCLLDIGGGTTD
                     IAVFTHGAIRHTAVIPIAGDQITNDIAMALRTPTKDAEDIKCRFGCALRSMADPHHMV
                     EVPGVGDRGTRQLSRQTLAEVIEPRVEELYTLVQAELRRSGFEELLSSGIVMTGGSSS
                     LQGMVELGEEIFHMPVRLGLPRYHGSLEEVVLTPRCSTGIGLIESGMEQLRRNQHTRL
                     EGNSFKRILGRMRSWFQVNF"
     gene            complement(4190..4888)
                     /locus_tag="VFI43_05795"
     CDS             complement(4190..4888)
                     /locus_tag="VFI43_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HET7061673.1"
                     /translation="MWDNHQMLGSLANSLFALAVLIASYYITKWVVNLPLLPLKEVSV
                     SSTGTGRLKHVTHEQITDSVRSQAIGNFLTVDLDAARRAFGKLPWVRTASVRRVWPDS
                     LELKIEEHVVLARWGNRRLVNIRGEIFEAASDEPLPLFEGMDESAGDMARHYAIFSEL
                     LRPIGENIGYMRLSPRRAWTLRLGNGTTLELGREQLEARLRRYVLAHSHGFGKTAMQL
                     GYVDLRYPNGFAAR"
     gene            complement(4878..5795)
                     /locus_tag="VFI43_05800"
     CDS             complement(4878..5795)
                     /locus_tag="VFI43_05800"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7061674.1"
                     /translation="MSQDDFGKVAVLLGGRSAERSISLESGRAVLHALRRRGVDAHPF
                     DPSEQPMEALVQQGFTRAHIALHGRYGEDGTVQGALELLGLPYTGSGVLASALAMDKW
                     RTKLLWEAGGISTPRHTLLTEQSDFDAVAEELGLPLIVKPSREGSTIGLTKVEAAKDL
                     RSAWRLAAQHDPLVLAEQFVEGVELTASILGDVTLPLVRIQPMHGLYDYEAKYVANTT
                     QYFCPSGLSASEEYAIQAEAMKAHQMLGCEGWGRVDVMLDRSGTPYFLEANTSPGMTS
                     HSLVPMAASTAGISFDDLVMRILELAHVG"
     gene            complement(5822..6790)
                     /gene="murB"
                     /locus_tag="VFI43_05805"
     CDS             complement(5822..6790)
                     /gene="murB"
                     /locus_tag="VFI43_05805"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7061675.1"
                     /translation="MDMSEVESAHSQPVLRGEVRMNEPMRRYTSWRVGGAARRIYFPA
                     DLTDFAGFLQTLEQDEPVYVVGLGSNLLVRDGGVHGTVVVLHARLNELRFEQRLDRSG
                     LIYAQAGVACAKAARFAASHNLAGAEFLAGIPGTIGGALAMNAGCYDGETWEIVERVQ
                     TVSRKGRVRERPASDYGIGYRRVTLKEDDQTGKWTGEPLESEEWFTGAWFRLPDGVQA
                     ESRQRIKEFLGKRIASQPLNLPNAGSVFRNPVGDKAARLIEQCGLKGFQVGGAMVSPK
                     HANFIVNTGTAIAADIEAVIMAVQKGVREKAGIELEPEVRIIGRAL"
     gene            complement(6790..8316)
                     /gene="murC"
                     /locus_tag="VFI43_05810"
     CDS             complement(6790..8316)
                     /gene="murC"
                     /locus_tag="VFI43_05810"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7061676.1"
                     /translation="MKHKVKRVHFVGIGGSGMSGIAEVLLNLGYKVSGSDLCESVITR
                     RLKKLGATIHIGHESSHVASADAVVASTAVGPDNPEVVAARERNVPVVPRAIMLAELL
                     RLRQGIAIAGTHGKTTTTSLIASILAEAGMDPTFVIGGRLEAAGSHAKLGRGEFIVVE
                     ADESDASFLYLQPVLAVVTNIDADHMETYGHDFAKLKQSFVDFVQHLPFYGMAVLCVD
                     DLRVREIMPSITKPVTTYGLSDSAQVRAADIQHNEGQMRFCALVGVNGSTRRLNITLN
                     LPGLHNVQNALAAIAVCNEIGLPDRAIIKALAGFRGVDRRFQRYGEVALAATADRAEG
                     SFTLIDDYGHHPAEMAATIAAARGAFPGRRLVLVFQPHRYTRTRDLFEDFVKVLSSPD
                     VLLLTEVYPAGETPLVAADSKSLARALRVQGKIEPIFVETVDELPEEIHGVSRDRDVV
                     LVMGAGSVGAVAPALQAEAAHGESQPAQEKHSQDKRRRPVEKAEISLDKPEDCREQGC
                     "
     gene            complement(8412..>8800)
                     /locus_tag="VFI43_05815"
     CDS             complement(8412..>8800)
                     /locus_tag="VFI43_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7061677.1"
                     /translation="NNYAEAQVEGELVNFINDMGARYADCDLVICRAGALTVAELAAA
                     GLASILVPFPFAVDDHQTSNARFLSDKGAAILVPQSELTSERLAELLMGFTREKLSEM
                     AMKARQLAKPDATRVVAEACMEVIRM"
BASE COUNT         1851 a         2803 c         2150 g         1996 t
ORIGIN      
        1 aaccgatcgt ttcggctgcc gaagaccttc ttgagcagat tgttcagcat gaaatcctaa
       61 ttaagtccac tatctgaaac gattgtccaa aaaataaggg gtgaggtttt tggcctcacc
      121 cccgccgcag agtgctaatc cgactcaacc gggcatcttc aagaatcgtg atggattttg
      181 tggtaagccc ctgtgcctga cttcgaaatg caaatgcggg ccagtagaac ggccagtgct
      241 gcccacttcc gcgattttct gtccgcgcac taccacctgg cccactttga ccaatcgctt
      301 cgatgcgtgg gcatagcggc taaccaaatc gttgccgtgg tcaacgtcaa tcatattacc
      361 atattcgggg tgataatcgg aatacaccac cacgccaccc gctgccgcca cgatcggtgt
      421 tcctgctgcg gcggtaaaat cgaccccttc gtggaaagcg cttctgcccg aaaacggatc
      481 tatccgcacg ccgaacccgg aggaatacca tttcgcgttt actggcatca cggatggaag
      541 catttttttc ttgagcctgt cctgcatcaa cagggaatcc agagcaccga gcttgtcgct
      601 gcgatcttcc agaaggcgcg acaattcatc gattttatta ttgaattcaa taaaggacat
      661 ttcctgggat ggcagggtgg gcacaacgcc tccctgtccg ggtgcctgat tgaagagaaa
      721 ttcctggggt ttgatgccgg acagttctgc aagccgtgcc ccgaccgagt ccagccgcaa
      781 cagttgggcc tgcatctgac ccatcctcac ggccatggca ttgaggctgt cgcgaagata
      841 agattgcgct ttctgatgtt cttcctgctg tacgctcaga atgagcgacc gaagcgtggg
      901 actatcaatt ttatcggcgt agcgcaggga taggtagttc aaccccattg cgagcaatac
      961 ggcagtcaga aagaatacaa ctgcgacgag cacaacgtga gcgcttgtta acgtcaagct
     1021 tcttgttttt gccagattat tggaaattag aataatattc atctcattcc ctcatcttca
     1081 tttggccgat gcctccccac aagatcaact tttacctccg cctcctgagc cagggcgccg
     1141 atcatcagcg cttgttcagc cacatgaccg cattaaccgc aatgcaacat gtattctgcg
     1201 ggattgcccc gcctcgactg gctcgccact gcgccctcgg tggactttct aatggaaagc
     1261 tgatcatatt cgcccgtaac agcgccattg cggcaaagct caggcaaacg acgccttctt
     1321 tattgttaaa atttcaggcg aagggctacg aggttactgc aattcgaatt gccgtgcaag
     1381 caaattcagg tacccatggg aataacacgc cggctaccaa acgcagcatt ggcggggtgg
     1441 gcagggaaag ccttcgtcag ctcgccgtga agcttcccca atctcccctc aaagcctcgg
     1501 tcgagtcgct ccttcacaag gccagcactg ccggccggaa gacagggggt tgaacgaact
     1561 ttaacgaacg tctggacaag tttaaggaac gtctgaacaa gtcctcgcgg accgcatgca
     1621 ataatcctta atccgcttgt ttccggagaa atgccggaat atccagcatg tcgactccgg
     1681 attgtttcat cgcctccacg gttgcgtccc gccttctgtt cgtgcgaatc gccgccggcg
     1741 cgtccatttc tttccagttc acaccttctg caaataatgc gttgtccgtt ccggtgcgtc
     1801 cgtgaacaat actgaacggt tttgacagag cacggttggc tacgcccccc aacccggtag
     1861 ccaccatggt gactctaaga tcctcctgca tgccttcatc aatgacagtg cccacgatca
     1921 ctgtggcgtc ttcggcagtg aagttcctga tgacgtccat gacttcatga atctccttca
     1981 tcttcattcc tgcgctggca gtgatgttga cgagtacgcc gcgcgcgccg gaaaggttta
     2041 cgtcttcgag cagcgggctt gccacggcct gctccgccgc cactcgggcc cgatctgctc
     2101 cgcctgcaaa cgcggagccc atcatcgcca tgcccatctc ggacattacc gtttttacgt
     2161 cagcaaagtc gacattgacg agaccgggac agttgatgac ctctgcaatt ccggcaaccg
     2221 ccccgtacag tacattattc gcggccttga acgcgtcgag catgctgaca tcctcgccca
     2281 gcacagccat cagcttgtcg ttcggaatca cgatcaacga atcaacgtac tgcgacaatg
     2341 cctccatccc ggcctgcgcc gccttgatgc gtcgcccttc gaaggaaaaa ggcttggtaa
     2401 ccactgctac ggtcagtatg cccaactccc tcgcgacctg ggcaattacc ggcgctgcgc
     2461 ctgttccggt gccacctccc attccggccg tgaggaacag catgtcggcc ccctcgatca
     2521 attcggctat ccgctcgcga tcctccaacg cggcgttgcg tccgatttcg gggtcggctc
     2581 ccgccccaag ccccttggta atcgccgatc ccagctggag taaagtgcgc gcctggttgc
     2641 gcttcaatgc ctgggcatcc gtattggcgc atatgaattc gacaccttgc accccattca
     2701 ggatcatgtg atccacggca ttccccccgc atccgccgat gcctatgact ttgattaccg
     2761 cttcctgtgt ttgtgtatcc atgatttcaa acatggcagt tctccttata taattttcca
     2821 tcaaatccat tacggaccat tcagtcccgc cgtttgccct ctctgtgcag ctcttcgttg
     2881 ccccactaaa aattgacctg gaaccagctg cgcatccgcc cgaggatgcg cttgaacgag
     2941 ttcccctcga ggcgagtatg ctgattgcgc cgcaactgct ccattccgga ctcgatgaga
     3001 ccaatgcctg tggagcaccg cggcgtaagt accacttctt ccaagctccc gtgatagcga
     3061 ggcagaccca gtcgcacggg catatggaat atctcttctc caagctccac cattccctga
     3121 agagaactgc tgccgcctgt catcacgatg cccgaagaaa gcaactcttc aaagccgctc
     3181 cggcgcagct cagcctgcac caaggtatag agctcctcca cccgtggttc gatgacttcc
     3241 gccagcgtct gcctggaaag ctggcgtgtc ccccggtcgc ccacccctgg aacttcaacc
     3301 atatggtgcg gatcagccat tgaacgcagc gcgcagccaa accggcactt gatgtcttcg
     3361 gcatcctttg taggcgtgcg gagcgccatt gcaatatcat tcgtgatctg atctccggca
     3421 attggaatca ccgccgtgtg acggattgcc ccgtgagtga atacagcgat gtcggttgtt
     3481 cctcccccga tatccaatag acagacaccc agctcctttt catcttcgga cagtacggcc
     3541 aacgatgagg caagaggctg cagcacgaga tcacgcactt ccaggccgca acggcgtacg
     3601 catttcatga tgttctgtgc agccgcgacg gcaccagtca cgatatgcac ctttacttcc
     3661 agccgtatcc cgctcattcc gatgggttcg cgcacgtcct cctgcccatc cacgatgaat
     3721 tcctggttaa gcacatgaag aacctgctgg tcattgagaa tgttcaccgc ctttgccgtt
     3781 tccatgactc gctccacgtc cagccgcgat acttccttat cctttatcgc taccattcca
     3841 tgtgaattga agcttttaat atggctgccc gcaattccgg tatatacctc cctgatcttg
     3901 cagtccgcca tcagctcggc ctcctcaagc gcgcgctgga ttgcgtttac cgtagtgtct
     3961 atattgacca cgaccccctt cttcaaacca cgcgatggat gagtacccat gccgatcaca
     4021 tcaaatccac cttcgggttt tatctctgcg acaatggcta taatttttga tgtcccgatg
     4081 tccaggccga caatcagatt tttcgcttca ttgcctctac tcatctgccc gccaaattcg
     4141 caaatctcat tcttttatcc aagctgactc actccaggtc cggggggaac tatcgtgcag
     4201 caaaaccgtt tggatacctc aaatccacgt aaccgagttg catggccgtc ttgccgaacc
     4261 cgtgggagtg cgccaatacg tagcgcctca acctcgcctc caactgctcg cgccccagtt
     4321 ccaaggtggt tccatttcca agccgaagcg tccacgcccg ccggggagaa agtctcatgt
     4381 aaccgatgtt ctccccgatc ggacgcagca attccgaaaa aatcgcataa tgccttgcca
     4441 tatcccccgc actttcgtcc atgccttcga acaatggcag gggctcgtcg gatgcagcct
     4501 caaaaatttc tccccgaata ttcacaagcc tcctgtttcc ccatcgtgcc agcacgacat
     4561 gttcctcgat cttcaattcc aggctgtcag gccagacgcg ccgcacgctt gccgtgcgca
     4621 cccacggcaa cttgccgaat gcgcgacggg cagcatccag atcgaccgtc aaaaaattcc
     4681 caatggcctg gctgcggacc gagtccgtga tttgttcgtg tgtcacatgc ttgagtcgac
     4741 cagtcccggt cgaactcaca ctgacctctt tcaacggaag caatggcaaa ttgacgaccc
     4801 acttggtgat gtaatagctc gctataagca cggccagggc aaacagcgag ttcgccagcg
     4861 agcccagcat ctggtggtta tcccacatgg gcgagctcca gtattcgcat gacgaggtca
     4921 tcaaatgaaa taccggcagt gctggcggcc atgggcacga ggctgtggct ggtcatcccg
     4981 ggtgacgtgt tggcctcgag aaaatagggt gttcccgatc gatccagcat cacgtcgacc
     5041 cttccccatc cttcgcaccc cagcatctga tgggctttca tcgcctccgc ctgtattgca
     5101 tactcctctg aagccgacaa accgctcggg caaaaatact gggtggtatt ggcgacgtac
     5161 tttgcctcat agtcgtacag tccgtgcatg ggctgaattc ttaccagggg cagggtaacg
     5221 tcgcccagga tagaggcggt cagctccacc ccttcaacaa actgttccgc cagcaccagg
     5281 ggatcatgct gggcagcaag gcgccacgcc gatcgcagat ccttggctgc ctctactttc
     5341 gtcaagccaa tggttgaccc ttcccttgac ggcttcacga tgagcggcag gccgagttct
     5401 tccgcaactg cgtcaaaatc gctttgctcc gtcagcaagg tgtgacgagg cgtgcttatc
     5461 cctcctgcct cccacagcag cttggtccgc catttatcca tcgcaagcgc acttgccagg
     5521 acaccgctcc ccgtataggg aagtcccaag agctcgagcg cgccctgaac tgttccatct
     5581 tcgccatagc ggccgtgaag agcgatatgg gcgcgggtga atccctgctg cacgagcgcc
     5641 tccatcggct gctcggaagg atcgaaagga tgcgcatcga ctcctcgccg gcgcaacgca
     5701 tgaagtactg ccctgccgct ttcgagggaa atactgcgct ctgccgaccg gccgccgagc
     5761 agcacggcaa cttttccgaa atcgtcctga ctcattgcca ttttttttaa agacctgcgg
     5821 actatagcgc ccgcccgata atccgcactt cgggctccag ctcaatccct gccttttctc
     5881 ttaccccttt ttgtaccgcc atgatcactg cctcgatatc tgcggcgatc gctgtccctg
     5941 tgttcacaat aaaattcgca tgcttgggtg aaaccatcgc acctcccacc tgaaacccct
     6001 tcagtccaca ctgttcaatc aaccgggcgg ccttatcgcc caccgggttt cggaaaacag
     6061 aacctgcatt gggcaggttc aaaggctggc tcgcgattcg tttaccgagg aattccttga
     6121 tgcgctgacg cgattcagcc tgcacaccat ccggcaatct gaaccaggct cctgtgaacc
     6181 attcctcact ctccaacggc tcccccgtcc atttcccggt ctggtcgtct tcctttaatg
     6241 tcacacggcg atagccaatt ccgtaatcgc tggcgggacg ttcacgcact cgtcctttgc
     6301 ggctgactgt ttgcacgcgc tcaacaattt cccaggtttc accgtcatag catcccgcat
     6361 tcattgccag ggcaccgccg atagtccccg gaatgcctgc cagaaattcg gcacctgcca
     6421 agttatgtga tgcggcgaac cgggcagcct ttgcgcatgc cacgccagcc tgtgcataaa
     6481 tcaacccgct tctgtcaagc cgctgctcga accgcaactc gttgagccgc gcgtgaagta
     6541 ccacgacggt accgtgcaca ccgccatcgc gcacgagcag gttactgcca aggcccacta
     6601 catacaccgg ctcgtcctgc tccaaagtct ggaggaaacc ggcgaagtcg gtgagatcgg
     6661 cggggaagta gattcgtcgg gctgctcccc cgacacgcca cgaggtgtac cgtcgcatcg
     6721 gctcgttcat gcggacttcc ccgcggagaa cgggctgtga atgagccgat tcaacttcgg
     6781 acatatccat cagcacccct gttctctgca gtcttccggt ttatcgagcg atatctccgc
     6841 cttttccacc ggcctccgcc tcttatcctg gctatgcttc tcctgcgctg gctgactctc
     6901 gccatgggca gcttccgctt gaagcgcagg agcaacggcg cccaccgacc ccgctcccat
     6961 taccaggacg acatccctgt cacgcgacac gccgtggatc tcctccggca actcgtccac
     7021 ggtctcgacg aagatgggct ctatcttccc ctgcaccctc aaagcgcgcg caagcgactt
     7081 gctgtcagcc gctacgagag gggtttcccc cgcgggatac acctcggtca gcagcaatac
     7141 atccggactg gataacactt tcacgaaatc ctcgaacaaa tcccgggtac gggtatagcg
     7201 atgcggctga aacaccagca caagccgtcg ccctgggaac gctccacgcg cggctgcaat
     7261 cgtggctgcc atttcagcag ggtgatggcc gtaatcatcg ataagtgtaa agcttccctc
     7321 cgcgcggtct gcggtggcgg ctaacgcaac ttcgccgtac cgctgaaagc gtcggtccac
     7381 gcccctgaaa ccagccaatg ccttgatgat tgccctgtcc ggcaacccga tttcattaca
     7441 caccgcaatc gctgcaagcg cgttttgcac attgtgcaat ccgggaagat taagcgttat
     7501 attcagcctg cgcgtgctgc cgttgactcc caccagcgcg caaaagcgca tttgtccttc
     7561 gttatgctgg atgtcggcgg cgcggacctg agctgaatcc gacaggccgt aggtcgtgac
     7621 gggtttggta attgagggca tgatttcgcg aacccttaga tcatcgacac acaagacagc
     7681 catcccgtag aaaggaaggt gttgcacaaa atccacgaaa gattgcttca gcttggcgaa
     7741 gtcgtgtcca tacgtttcca tgtgatcggc atcaatattg gtcacgaccg cgagcaccgg
     7801 ctgcaagtag agaaacgagg cgtcggactc gtccgcttca actacgataa actcgccgcg
     7861 gccgagcttc gcgtgcgatc ctgctgcttc cagccttccc ccgatcacga atgtcgggtc
     7921 cattccggct tcggccagaa tgctggcaat cagactggtc gtcgtggtct tgccatgcgt
     7981 acccgcgata gcgattccct ggcgcaatct cagcagttcc gccagcatga ttgcccgcgg
     8041 cacaaccggc acgttccgct cccgcgccgc gacaacttcc gggttgtccg gccccacggc
     8101 ggtggacgct acgaccgcat cagcggatgc gacgtgactg ctctcatggc caatatggat
     8161 agtcgcgccc agcttcttga gccggcgggt aatcacgctc tcgcacaggt ctgatccgct
     8221 gaccttgtac cccaggttca gcagaacctc agcaattcca ctcatccccg aaccgccgat
     8281 gccgacaaaa tgcacgcgct tcaccttgtg cttcatatcg gaccgccgaa gacatcaaat
     8341 aatctcgaaa acactacctt tgctcctcat ccttcttatg gtcagcctat gggtccgcct
     8401 acctccctgc cctacatcct tatcacttcc atgcatgctt ccgctacaac tcgagtcgca
     8461 tcgggcttgg caagctgccg tgccttcatg gccatttcag aaagtttttc ccgtgtaaat
     8521 cccatcaata attccgccag cctttcggat gtcagttcgc tttgcggaac caggattgcg
     8581 gcgcccttgt cgctcaaaaa tcgtgcgttg gatgtctgat ggtcatccac cgcaaatgga
     8641 aatgggacca ggatactggc cagtcctgcc gcggcgagtt cagcgaccgt cagagcgccc
     8701 gcccggcaga tcaccagatc gcagtcggcg taacgcgctc ccatatcatt gatgaaattg
     8761 accagttcgc cctcgacctg ggcctcggca taattgtttt
//
LOCUS       DASSHJ010000149        11778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_674063, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000149 DASSHJ010000000
VERSION     DASSHJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674063"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFI43_05820"
     CDS             <1..581
                     /locus_tag="VFI43_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET7061678.1"
                     /translation="AGAGDILDKAQVCENLEQALSGTVFAAAVTARRRDLSHEVFDAR
                     RGAIKLISHAVQDSVAVVFGTEISGLTNQEVSKCQVVVHIPADPYYSSLNLASAVQVL
                     AYELRMASLETGPSPQTVSTRNPVTFDDIELLLGHLERVAIRSGFLDPEEPKRLMQRM
                     RRLLARARLEREELNILRGILSACEKQMQNRL"
     gene            827..1312
                     /gene="iscR"
                     /locus_tag="VFI43_05825"
     CDS             827..1312
                     /gene="iscR"
                     /locus_tag="VFI43_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HET7061679.1"
                     /translation="MRLTTKGRFAVTAMIDLALHCGEGPVTLADISERQKISLSYLEQ
                     LFGKLRRNKLVGSVRGPGGGYCLTKPMRDISVVDIILAVDEPIDATQCGGKENCHNDQ
                     RCMTHDLWATLNARIRDYLALVTLEQLVANPKVKDIAVLQDMRAHKQPHPTERYPTAL
                     M"
     gene            1406..2563
                     /locus_tag="VFI43_05830"
     CDS             1406..2563
                     /locus_tag="VFI43_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET7061680.1"
                     /translation="MTYAYLDHNATTPVDDAVFEAMLPYFKDRFGNASSRHALGMVAR
                     RAVNQAREQVAEALGVQASQVIFTSGGSESNNLFIQGAAGYMKPSRIVISSVEHPCVL
                     RTAKELARNSNPGWNLRLLAVDDLGRVDPEDAEKAMQEQKPGLVSVMLANNETGVIQD
                     VAAIAEKAREHGAVVHTDAVQAFGKIPVDFESLNVHALTLSAHKVYGPKGAAALVVDK
                     RLLLKPLIHGGGHENGMRSGTENVPAIVGFGVACTLAGERMMAATEKILAMRERLEQG
                     LMAIGAVIFGYGALRLPNTCYFALPGIEGDTLVIRLDKAGFAVASGAACSSVNPGRSH
                     VLEAMEVDPALTRCAVRISLGSTNSMTEVEDFLRALKRGVDELRRMSMVTA"
     gene            2560..3411
                     /locus_tag="VFI43_05835"
     CDS             2560..3411
                     /locus_tag="VFI43_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HET7061681.1"
                     /translation="MKPGSMSGARTGLILTNYVGLFYESVRRCPSRLLLLVIGAVLVS
                     GCASTRRHPTEDTSFDNIDPYEKANRKSYAFTDYLDRKVMQPIAEFYIDYVPVRVQHS
                     VGHFYDNLAYPNVVLNVFLQGKVRQGFQDSARFLINSTAGIGGLFDVASRIGLPQHDE
                     DFGQTLGVWGVDADTYIFIPMLGPSTNRDIASLPVRLVTNPLFFAGIFAVGAPVTVPL
                     SFLDAVDTRARLSGPMKIRDEAALEPYLFVRDGYLQQRRHLIYDGNPPPEVYEEMMDE
                     EVPASTK"
     gene            3529..3936
                     /locus_tag="VFI43_05840"
     CDS             3529..3936
                     /locus_tag="VFI43_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061682.1"
                     /translation="MGEYHLSSPIIAPPQSLTRVTHLIYFLHAFSVLMGVLGPALIIT
                     TFLSGWPSIIAVIINYIKRNDVRGTYLDSHFRWQIRTFWYALFWLVVAALLFATVVGI
                     VFAYILAVGVGFWIVYRVLRGWMALGDGRSAPA"
     gene            4377..4943
                     /locus_tag="VFI43_05845"
     CDS             4377..4943
                     /locus_tag="VFI43_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HET7061683.1"
                     /translation="MFTNNKKVLAVFQIKPKFLFLVALVGLLTGCSVINAQKGNPLSS
                     GRWIILPFQNYSQAPRAGEQMEELVATLFRIRGVSDIEIYQPQDEDKSGWLEFNDRRR
                     QEKALLSAIKNNVSYAVTGSVDEWQYKLGVGSEPVVGLTVRILEVPSGKVVWSASGAR
                     SGWSTGSLSGTAQKLVKELISSVEIKRP"
     gene            4947..6344
                     /locus_tag="VFI43_05850"
     CDS             4947..6344
                     /locus_tag="VFI43_05850"
                     /inference="COORDINATES: protein motif:HMM:NF028273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PelD GGDEF domain-containing protein"
                     /protein_id="HET7061684.1"
                     /translation="MLTAVMREKEAKMRWAEPIAISLLLPAIGYAIDASDPFFIAYRF
                     PWLVLAPVFSSLRYGFLSGVTSSALLIVMISAVFYQGLPQVPFFPNEMIVGMLLLTVI
                     SAEFNESWARKIKLLDHQYNHLKVRMDKFARSYHLIKGSHYQLEQHLASQVKSLRLSL
                     LDLKKKILSLENASGEPLAGIGENILKILGSYLNVQIAGIYAVNERREIGTNPVASLG
                     QPRPVSALDPVIAEAMRTGHVASVEVEKNDPAAVESLVAIPLMDVYQRIWGIVVVNEM
                     PLFSLQESTMDFLAVLGGKMGDLIKRRAESHCNDNGIPVFEGKLRRILEEVRDLKTSA
                     VAIAAVISSDELKGKLLSKFQTELRGVDEILIVHDTPDHQVFLMLLPYTDEKGGKEFL
                     KRVELSRLSPKTIYHRPDAAMCISDTNIRVCMWLLDNKSSLEKVLSGIDQFCTEGSTD
                     IKIRENQNEGVLHSA"
     gene            6319..7368
                     /locus_tag="VFI43_05855"
     CDS             6319..7368
                     /locus_tag="VFI43_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061685.1"
                     /translation="MKVFYILLESAFLALALGLEITVLQLVLSESLSGQNLLPALLGH
                     LVTSIVAAYILPKSMMLDLPDPLVARLKVYFLFFVIIFYLPVLGLIGLCLAVPFKALH
                     GHKREKPNSFMHTNKIRDFPSESAVAGGPAVNLHSLNDLYRSRNPERRLQAVYATLKL
                     KDRDAIPLLRTALADPIDDIRLLAYTLMDRKEYRLSKHIENTKQELEKKENSGRKQLY
                     RQIASYYWELAHLGLVQGEAKNHVLGMAYKYIELGLEYSPEDCGLRFQYAQILLRLGK
                     YQLAFEQFKKAEILGIEHVSLLTYYAEIAFHNRRYYEVKQLMTAIELPAAYPLLTTAA
                     RFWQMRSNATATEIR"
     gene            7343..8854
                     /gene="pelF"
                     /locus_tag="VFI43_05860"
     CDS             7343..8854
                     /gene="pelF"
                     /locus_tag="VFI43_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HET7061686.1"
                     /translation="MQQQPKYADITLLLEGTYPFIRGGVSSWVHQIIEGLPQYTFSII
                     FLGSRKSDYSGLQYTLARNVVHIESHYLWDTFFSSPPKACKGNEAYLEEAAKLHDWFR
                     NPCSDFQESDLKRLLVSLGEPSGFTAEEFYFSDASWRHICEYYSRYNEGNSFIHYLWT
                     IKSTHIPLFMLAGIARRFSMATGAFHSISTGFAGFLGVLLNYRTARPLILTEHGIYTK
                     ERKMDLQTLFIKEHRDSLSDMLYMGMQYQELLWIRYFESLGRLIYKASNPIISLYESN
                     RKRQIADGAERARTQVIPNGMEMDRFRPARAQRPEKIPLVIGFLGRIVPIKDVKTFIR
                     AMRTVLAQLPEAEGWLIGPQEEDKEYVEECKSLIQELGLEGKVIFLGFQTVTDILPRV
                     GVLVLTSISEAFPLVLLEAFASGLPVVTTDVGACREIIEGNSDEDRALGCAGIVTPIA
                     DPEATAEAVVTLLTDENRWRAATKAGLDRLEKYYTQSGMLDSYKKVYDDALMQ"
     gene            9081..10448
                     /gene="pelG"
                     /locus_tag="VFI43_05865"
     CDS             9081..10448
                     /gene="pelG"
                     /locus_tag="VFI43_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HET7061687.1"
                     /translation="MAGIGFRLRDLSKTGALSGLLAAYGFAGIISSGPWVLSIVGILL
                     IGIINIENAITPHYVGEFQISVTYLMAISLILTSPLQLMISRFIADKIYEHHDEQVLP
                     NLTGAILVVITIAGTLAGICLIYLFTGSVLYRLLMLSGLVILSIIWLVVIVLSGVRAY
                     REILLAFLLGYGITVLAAIELKAFGLEGLLSGFVLGQAVLLFMLLVVIFRSYSSNKFI
                     AFDFLRRSQIFPSLALTGLVYNVAIWADKFIFWFNPQTSVMVIPPLRASQIYDLPIFL
                     AYLSIIPGMAVFLLRMETDFAEQYKNFYAAVNGGGSLKLIMEMYEEMLLTIRRGFIEI
                     FKVQGMTIVILLAIGDKLLAWVGISPFYRILLNIDLVAVGVQVLLLAVLNILFYFDYR
                     KDALYLCLLCLGTNVAFTMLSQYLGPAFYGYGFALSVVLTTLVGMVVISKRLSRLVYQ
                     TFMLR"
     gene            10619..>11778
                     /locus_tag="VFI43_05870"
     CDS             10619..>11778
                     /locus_tag="VFI43_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HET7061688.1"
                     /translation="MFSFFNRYALVGLLQGMLLSSACSIASADPSTAFFYGRPVPVKL
                     LSHFEQVVVEPENMDDLDHLRDQGTRVFAYVSVGEVNWTRPWYSKIPASWFIAIDKEW
                     QSSVVDLTQQGWHDYLINNYLRGLWQQGYRGFFLDGLESYQKHVVEPDARLLQEKALA
                     NLIKRLHQHFPGVNLILNRGFAVLPEVAHYAVALVAESLFQRWEPSGQAYVKVIEPDR
                     YWLLNKLKQVRDQYGLQVIVVDYVDPKQKELARITAKKISELGFTPWVANPSLDILGI
                     GASEIFPRQVLALYDGQQYPEGLQRTELHKLLAMPLEYLGCTLDYLDVNLGLPGNLLS
                     GQYAGIVTWFNNDELLRPEVYREWLLRQMQSGVKVVVLGNLGFKADNSFLHHL"
BASE COUNT         2759 a         2827 c         3141 g         3051 t
ORIGIN      
        1 cggcaggcgc gggggacatt ctggacaaag cgcaggtttg cgagaatctt gaacaagctt
       61 tgagcggaac ggtatttgca gctgcggtca ctgctcgccg gcgtgacctt tcacatgagg
      121 ttttcgatgc gagacgcggg gcaataaaac tgatttcgca cgccgtgcaa gacagcgtgg
      181 cggtggtatt cggcacagaa atttcaggtc tgaccaacca ggaggtcagc aaatgccagg
      241 ttgtcgtgca tatacctgcc gatccgtact actcctcgct caatctcgcg agcgccgtcc
      301 aggtattggc atacgagttg cgcatggcgt cgctggagac ggggccttct ccccaaactg
      361 tgtcaactcg gaatcctgtg actttcgatg acatcgaact gctgctcggc caccttgaac
      421 gggtggccat acgaagcggt tttctcgatc cggaagagcc caagcgcttg atgcaaagga
      481 tgcgccggtt gctggcgcgc gcccgcctgg aaagggaaga gctcaatata ctgcggggaa
      541 ttctcagcgc ctgcgaaaaa caaatgcaaa atcggctata agacaatcgc tcaggcatgc
      601 actcctggcc tcaagagaga ttgtgacgcg ggtgacagtg ctcttcattg ccgcgaattc
      661 aaggctcgca tggcatcgat atcgtgctga aaatgggaag gtcataccca taaaatagtt
      721 gactgtaata aaggggcttt atatacttga ccaacgttgt cgggtattag agcggtcatt
      781 ttgagaacgt ggatctcgaa aatatgaatt tttattggag cctaaaatgc gattgacgac
      841 caaggggcgg tttgcggtaa ccgcaatgat cgatcttgcc ttgcattgtg gggaagggcc
      901 agtcacgcta gccgatatta gcgagcggca gaaaatctcc ctgtcttatc ttgaacagct
      961 gttcgggaaa ttgcggcgca ataaattggt gggtagcgta cgtggtccgg gcgggggtta
     1021 ttgcttgacg aagccgatga gggacatctc tgtcgtagat attattctgg ccgtggatga
     1081 gcctattgat gcaacccagt gcggcggcaa ggagaattgc cataacgacc agcgttgcat
     1141 gacacacgac ttgtgggcga cactcaatgc gcggatccgc gattatctcg ccctggtgac
     1201 cctggagcag ctggttgcga atcccaaggt aaaggatatt gccgtcttgc aggacatgcg
     1261 cgcccacaag cagccacacc cgacggagcg atacccgacg gctctcatgt gaaaatgctg
     1321 tgcgcgtgtt tcctgccctg atgggaagct tgttgcagcc ctgtgaatta acctttcccg
     1381 ctgaacaacc tcttatcgac cgcttatgac ttacgcatat ctcgaccata atgccactac
     1441 accggtggat gatgcggtgt ttgaggccat gctgccctac ttcaaggatc gattcgggaa
     1501 tgcctctagc agacacgcac tggggatggt ggcgcgccgg gcagtcaacc aggcgcgcga
     1561 gcaggttgcc gaggcgttgg gcgttcaggc ttcccaggtt attttcacga gcggtggatc
     1621 ggaatcgaat aacttgttca ttcagggcgc agctggctac atgaagcctt cccggatcgt
     1681 cataagttca gttgaacatc cttgtgtgct gcgaaccgcc aaggagcttg cgcggaactc
     1741 gaatccgggt tggaatctgc gcttgttggc cgtggacgat ttggggcgcg tcgacccgga
     1801 ggacgcggaa aaagcaatgc aggagcaaaa gcctggtctc gtctcggtaa tgcttgcaaa
     1861 caatgagacc ggagtcatcc aggatgttgc ggcgattgca gagaaggcac gcgagcatgg
     1921 cgccgtggtg catacggacg cggtgcaggc attcggcaag attcccgtcg acttcgagtc
     1981 actcaacgtg cacgccctga ccttgtccgc gcataaggtt tatgggccga aaggagcagc
     2041 cgcgcttgtc gtggacaaac gtctgcttct caagccgctt atccacggtg gcggccatga
     2101 gaatgggatg cggtcgggaa ccgagaatgt accggcgatt gtggggtttg gcgtggcctg
     2161 tacattggcc ggagaacgga tgatggctgc taccgaaaaa attctggcaa tgcgagagcg
     2221 tctggagcag gggttgatgg caattggcgc tgttattttc ggatacgggg ctttacggct
     2281 gcccaatacc tgttattttg ccttgccagg catagagggc gatacgcttg tcatccggtt
     2341 ggacaaggca ggatttgcag tagcgagcgg cgccgcatgt tccagcgtga atccaggccg
     2401 cagccacgtg ctggaggcaa tggaagtaga cccggccctg acccgatgtg cggttcgcat
     2461 cagcctggga agcacgaact ccatgaccga ggttgaagat tttttgcgag cgttgaagag
     2521 aggagtggat gaattgaggc gaatgagcat ggtgacagct tgaagcccgg cagcatgagt
     2581 ggcgcaagga cagggctaat cctcactaat tatgtgggat tattttatga atcggtcagg
     2641 cgctgccctt ccaggctcct gcttctcgtt atcggtgctg tactggtatc ggggtgcgcc
     2701 agcacgcgca gacatcccac ggaagatact tcgtttgaca atattgatcc ctacgaaaaa
     2761 gcaaacagga agtcgtacgc attcaccgat tatctcgacc gcaaggtgat gcagccgatc
     2821 gctgagttct atatcgatta tgttccggta agagtacagc attccgttgg gcatttttac
     2881 gataacctgg cctatccgaa cgttgttctg aacgtttttt tgcaaggcaa agtgcgacag
     2941 ggtttccagg acagcgcgcg ttttctgatt aactcaaccg ccggcatagg cggacttttc
     3001 gacgttgctt cacgaatcgg tcttcctcag catgatgagg actttggcca gacactcggg
     3061 gtatggggtg tcgacgcaga cacttatata ttcataccca tgcttggacc cagcaccaac
     3121 cgtgatattg cgagccttcc ggtccgcctc gttaccaatc ctttgttctt cgcgggcata
     3181 tttgctgtcg gcgcgccggt caccgtacct ctttcctttc tggatgcagt tgatacccga
     3241 gcgcgactgt cgggcccaat gaaaattcgg gatgaagcgg cgctcgaacc ctatctgttc
     3301 gtgcgggacg gctatctgca gcagcgcagg cacctgatct acgacggaaa tccgccgccc
     3361 gaagtatatg aggaaatgat ggatgaggaa gtacccgctt ccacgaagta gcagatcgag
     3421 tgtacggcaa cggcatcctg gaacagaatg aatgcttcgg gttgacgaag gctcgtactt
     3481 ttcaggctac cccccgcttc gtttaaatga gggatgaagg agatgaagat gggcgagtat
     3541 cacctaagtt ctcccatcat cgcgcccccg cagagcctca cccgcgtgac gcaccttatc
     3601 tacttccttc acgccttcag cgttctgatg ggtgttctcg ggcccgcgct catcatcaca
     3661 acctttctga gcggatggcc ctcaatcatc gccgtcatca tcaactacat caagcgaaac
     3721 gacgtacgcg gcacctacct cgattcccat tttaggtggc aaattcgaac attctggtac
     3781 gctctattct ggctggtcgt agcagccctt ctctttgcca ctgtcgtagg gatcgtgttt
     3841 gcatacatcc tcgcggtagg cgtcggcttc tggatcgttt atagagtatt gcgcgggtgg
     3901 atggctctcg gggacggcag gagcgcgcct gcataggtgg ggctgttaac acttgtttcg
     3961 tgcccgcgac ccttcttcgg taatatttct tcttttttgc ctccaatctg atggaagcgc
     4021 atttataaag tactctactg tctgtcattt accaagcctc ccccttcttc ccggaagcct
     4081 tttcggtccg ggctaatcaa tcccaggttt tttgcagggt tttcatgctt tcggccgatc
     4141 tgtatcggct gccctccagc cctcgttttc tcaacccctt tttccggttt ttcgctgcag
     4201 cggaactatg ctgtttgcgt tatgtggggt gacgcacaga ttcagttagt ctgtatgctg
     4261 aaaactcatc atttcgtaga cctgaaaaat tgttgggaca gggcaacgaa tgaaacgggg
     4321 ccgggctata aatactgccc tcacttatta aataggcaat tagaaaaggt cgatttatgt
     4381 tcaccaataa caaaaaagtg ctggcggttt ttcagatcaa gcctaaattc ttgtttcttg
     4441 ttgccctggt tggcttgctg acgggatgtt cggtgatcaa cgcgcagaaa ggcaacccgc
     4501 tctcttccgg gcgatggatc attctacctt ttcagaatta ctcccaggcg ccccgggccg
     4561 gggagcaaat ggaagaactg gtggcgacat tgttcagaat tcgaggggtt tcagacatag
     4621 aaatctacca gccgcaggac gaagacaaga gcgggtggct tgagttcaac gatcgccggc
     4681 ggcaggaaaa agcgttgttg tcggctatta aaaataatgt ttcttacgcg gttaccggaa
     4741 gcgttgacga atggcaatac aagcttggtg tgggcagtga acctgtggtt ggcctgaccg
     4801 tccgtatcct cgaggttccc tcggggaaag tcgtctggtc tgcaagcggt gcgcggtctg
     4861 gctggagcac gggctcgctg agcggaaccg cgcagaaact ggttaaggag ctgatttcga
     4921 gtgtggaaat taagaggcca tagcccgtgt tgactgcagt gatgcgtgag aaagaagcaa
     4981 agatgaggtg ggcagagccc attgctatct ctttattgct gccggcgatc ggatacgcca
     5041 tcgacgcctc tgatcctttt ttcatcgctt accggtttcc ctggctggtg cttgcgcctg
     5101 ttttctccag tctgcggtac gggtttttat ccggcgtcac ctcttcagct ttgctcatcg
     5161 tcatgatttc cgcggtattt taccaggggc tccctcaagt tccgtttttc cccaacgaaa
     5221 tgatagtcgg aatgttgctg cttaccgtga tatcggctga attcaatgaa tcatgggcgc
     5281 gcaagatcaa gcttctcgat caccagtata accatctgaa ggtgagaatg gataagttcg
     5341 cccgttccta ccatctcatc aaaggttctc attatcagct ggagcaacac ttggcgagcc
     5401 aggttaaaag cctgcgttta tctttgcttg atctgaaaaa gaaaatcctg tctcttgaaa
     5461 atgcgagcgg tgaaccgttg gccggtattg gcgagaatat tctcaagatt ctgggcagct
     5521 atctgaatgt tcagatagcg gggatttatg ccgtaaacga acgtcgggaa atcggtacca
     5581 accctgtggc ctccctgggg cagccgcgcc ccgtatccgc gctcgacccg gtaatagcag
     5641 aagcgatgcg gacggggcat gttgcaagcg tggaagtgga aaaaaatgat ccagccgctg
     5701 ttgaatcttt ggttgctatt cccctgatgg atgtctatca gagaatctgg ggcattgttg
     5761 tcgtaaacga gatgccgtta ttttcattac aggaaagcac catggatttt ctcgctgtcc
     5821 tggggggcaa aatgggcgat cttattaaaa gacgggcgga atcgcactgt aatgataatg
     5881 gaatacccgt tttcgaaggc aagctgcgga gaatcctgga ggaggtgaga gacttaaaaa
     5941 catctgcagt tgcgatcgca gctgttatca gctctgatga actgaaaggc aaacttcttt
     6001 cgaagtttca gaccgagctg aggggggtgg atgaaatcct tatcgttcac gatactcctg
     6061 atcaccaggt ctttctgatg ttattgcctt ataccgatga aaagggtgga aaggaatttt
     6121 taaaacgtgt cgaattatcc cggctgtcgc cgaaaacgat ttaccacagg ccggatgcgg
     6181 ccatgtgcat tagcgatacc aatatcagag tttgcatgtg gcttctggat aacaagagct
     6241 cattagaaaa ggtgttgtca ggaatagacc agttttgcac ggaggggtcc acggatataa
     6301 agatcaggga gaaccaaaat gaaggtgttt tacattctgc ttgaatcggc ttttctcgcg
     6361 cttgccctgg ggttggaaat aactgttctg cagttggtct tgagtgaatc cctatcggga
     6421 cagaacctgc tgccagcctt gctcggtcat cttgtgacaa gcattgtcgc tgcttatatt
     6481 ctgccgaaaa gcatgatgct tgatctccct gatccccttg ttgctcgtct gaaggtttac
     6541 tttctgttct ttgtgatcat tttctatttg cccgtattgg gactgatagg actgtgcctg
     6601 gccgtgccct ttaaagcgtt gcatgggcat aagcgggaaa agccgaattc attcatgcat
     6661 acaaacaaga tacgggactt tccttccgaa tccgctgttg ctggcggccc agctgtcaac
     6721 ttgcatagcc tcaacgattt atatagatcc aggaatcctg aaagacggtt gcaggctgtg
     6781 tatgcaacct tgaagttaaa ggatagggat gccatcccgc tattgcgcac agcgcttgcc
     6841 gatcccattg atgacatcag gctgcttgcg tacactttaa tggatcgtaa agaatacaga
     6901 ttgagcaagc acattgaaaa taccaagcaa gagcttgaaa aaaaggaaaa ttcgggaaga
     6961 aaacagttat atcgccagat tgcgagttat tattgggaac tggcgcacct tggactggtt
     7021 caaggagagg caaaaaacca tgttctcggt atggcctaca aatacatcga actgggcctg
     7081 gagtattctc cggaagattg tggactgcgt tttcaatacg cacagattct gctgcggttg
     7141 ggaaaatacc agctggcgtt tgaacagttc aagaaggccg agattctcgg aatcgaacac
     7201 gtcagcctgc tgacctacta tgccgaaatc gcttttcaca atcgtcgtta ctatgaggtg
     7261 aagcaattaa tgacggcgat agaacttccc gcagcttatc ccctgttaac caccgcggca
     7321 cggttctggc aaatgagatc taatgcaaca gcaaccgaaa tacgctgaca tcacactatt
     7381 gctggaaggc acctatccat ttatccgggg cggagtttcg agttgggtac atcaaataat
     7441 agaaggtctc ccccagtaca ccttttccat aatttttctc ggatcgagaa agagcgacta
     7501 tagcggcttg caatatactc tagcgcgcaa cgtcgttcac atagagtccc attatctgtg
     7561 ggataccttc ttttcctcgc ctcccaaggc ctgtaaaggt aacgaggcat atctcgagga
     7621 agcggccaag cttcacgact ggttccgcaa tccgtgctcc gattttcaag agtcggacct
     7681 gaaaaggctt ttggtttctc tgggtgagcc gtccggcttc actgcagaag agttctattt
     7741 cagcgatgcg tcctggcggc acatctgtga atattacagc cgctacaacg aggggaattc
     7801 cttcatccat tacctttgga ccatcaaatc cacccatatt cctttattca tgcttgccgg
     7861 cattgcccgc aggttttcaa tggcaaccgg cgcgttccac agtatctcca caggctttgc
     7921 aggtttcctc ggcgtattgc tgaattatag aacagcccgg ccacttatcc tgacggagca
     7981 tggcatctat accaaggaaa gaaaaatgga tttgcagact ctttttatta aagagcatcg
     8041 cgattcattg agcgacatgc tttacatggg tatgcaatat caggaattgc tatggataag
     8101 atattttgaa tcccttggcc ggcttattta taaagcatca aatcccataa tcagcctcta
     8161 tgagagcaac agaaaacgtc agattgctga cggagcagag cgtgcccgga ctcaagtcat
     8221 tcccaatggc atggaaatgg atcgttttcg tcctgctcgt gcgcaaaggc cggaaaaaat
     8281 cccgctcgtg atcgggttcc tgggacgaat agtaccgata aaggacgtca agacttttat
     8341 tcgcgccatg cgaaccgttc tggctcaact gcctgaggct gaaggctggc tgatcggccc
     8401 gcaggaagaa gataaggaat acgtggagga atgtaaaagc cttatccagg agctggggtt
     8461 ggagggaaag gtaatatttt taggttttca aaccgttacc gatatccttc cccgggtggg
     8521 tgttctggtg ctgacctcga tcagtgaagc gtttccgttg gtgctgcttg aagcttttgc
     8581 aagtggtttg ccagtcgtga cgacggacgt aggtgcctgc cgcgaaatca ttgagggtaa
     8641 cagcgacgag gatcgggcgt tgggatgtgc tggcattgta actcccattg cagatccgga
     8701 agcaaccgct gaagccgtgg ttacgctgct cactgacgaa aaccgttggc gtgcagcaac
     8761 gaaagccgga cttgaccgac ttgaaaaata ttacacgcag tccggaatgc ttgacagcta
     8821 taaaaaggtg tatgacgacg cattgatgca atgagtcatt gcaaatcatc cggagcccgt
     8881 cgtcaatcaa atcctcctat tgttccaggg ctcagtgaat gtccatgacc aattaccata
     8941 gaccttgcgc cgctggattc tcagcgcatt gggccggata ctttctgttc cggctaacgc
     9001 aatgacccaa ctgcctttgt ccgacgtaga gcttctgaac tctaccgtca gccctcacac
     9061 aagttcccca tgaatcaata atggctggca ttggctttag attacgagat ctctcgaaaa
     9121 cgggagcgct ttccgggctt ttggcggcct acggttttgc cggaattatc agctcgggcc
     9181 catgggtctt atcgatcgtt ggcatcctat tgattggaat tatcaacata gagaacgcca
     9241 tcacgcctca ttatgtcggc gagttccaga tatccgtgac ttacctgatg gccatctccc
     9301 tgatcctgac cagccccttg caactgatga tttcacgatt tatcgcggat aagatatatg
     9361 aacatcacga tgaacaggtt ttaccaaatc ttacgggcgc aatccttgtt gtaataacaa
     9421 tcgcagggac gctagcagga atctgcctga tttacctgtt tacaggatcc gttttgtacc
     9481 gcttgttgat gctgagcggt ctggtcatcc tttccatcat ctggctggtg gtcattgttt
     9541 tgtccggagt gagggcttac cgggaaattt tattagcgtt cctgctcggt tacggaataa
     9601 ctgtattggc tgccatcgag cttaaggcgt tcgggcttga aggcttgctt tccggttttg
     9661 tcctgggtca agcggttttg ctgtttatgc tcctggttgt gattttccga tcttattcaa
     9721 gcaataaatt cattgcgttc gactttttgc ggcgaagcca aatattcccg agccttgctc
     9781 ttaccgggct tgtttacaac gtcgcaatct gggccgacaa atttattttc tggtttaatc
     9841 ctcaaaccag cgtcatggtc attccacccc tgagagcttc gcaaatctac gatcttccca
     9901 tatttctcgc gtatctatcc atcatacccg gcatggcagt tttcttgttg cgaatggaga
     9961 cggattttgc ggaacagtac aaaaactttt atgcggccgt taatggcggg ggttccctga
    10021 agcttatcat ggagatgtac gaagaaatgc tcttgacgat tcgaagggga tttatcgaaa
    10081 tcttcaaggt gcagggaatg accatcgtaa tactgctggc aataggagat aaattacttg
    10141 cctgggtagg aatatctcct ttctacagga tcctgctgaa catcgatctc gtcgcggtag
    10201 gtgttcaagt gctgttatta gctgttctta acatactgtt ctattttgac tacagaaagg
    10261 acgcgttgta tctctgtttg ctgtgcctcg gcacgaatgt tgcgttcacg atgctgagcc
    10321 aatatctagg acctgcgttt tacggttatg gcttcgcact atctgttgta ttgacaacat
    10381 tggttggaat ggttgtgatt tcaaaaaggc ttagccgtct ggtttatcag acctttatgc
    10441 tccgttaggc agttatgctg ggatgaatca actccgcact cccgtccgat tgtttctgcc
    10501 cgctatgaat ggaatcgata acctggaacc ttcgtcgaaa gacccaaaaa accggatcga
    10561 agatgggcgg gaatacctgc aaaccgccgt ataccaatag cgcaggaacc aagagcccgt
    10621 gttttccttt tttaatcgtt atgcacttgt agggcttctt caaggtatgt tgctatcttc
    10681 cgcatgctcg attgcatcgg ctgatcccag cacggcattt ttctatggcc ggcctgttcc
    10741 tgtaaagtta ctctcgcatt tcgagcaggt tgtagtagag cccgagaaca tggatgattt
    10801 ggatcatctt cgtgatcagg gtaccagggt attcgcttat gtaagcgtcg gagaagtgaa
    10861 ttggacgcgg ccctggtatt cgaaaatccc tgcaagctgg ttcatagcga tcgacaaaga
    10921 gtggcaaagc agtgttgtcg acctgactca gcaaggatgg catgactacc tgatcaataa
    10981 ttatttgagg gggctatggc aacaaggcta tcgcgggttt ttccttgacg gactggagag
    11041 ctatcaaaaa cacgttgttg aaccagatgc ccggttgctg caggaaaagg ctctcgcgaa
    11101 cctgatcaaa cgcctgcatc aacattttcc tggggtgaat ttgatactta accgcgggtt
    11161 cgcagtcttg cctgaagttg cccactatgc ggttgcgtta gttgccgaat ctctgttcca
    11221 acgttgggaa ccatccggcc aggcatatgt gaaagtaatc gagcctgacc gctactggtt
    11281 gctcaacaag ttaaagcagg tgcgggatca atatggatta caggtgatcg tcgttgacta
    11341 cgttgatcca aagcagaagg aattagcgcg aattactgcc aaaaaaatat ccgagctagg
    11401 ctttacgcct tgggtggcaa atccctcact ggatattctt ggcattggcg cgtcggaaat
    11461 tttccccagg caagtgctgg ctctctatga tgggcagcaa tacccggaag ggttgcagcg
    11521 gactgagctt cacaagctac tggcaatgcc tttggagtac ctgggttgta cgcttgacta
    11581 cctggatgtc aatcttggat taccgggaaa tctgctgtca gggcaatatg caggcatagt
    11641 tacttggttc aacaatgacg aattattgag gccggaggtt taccgggaat ggctattgcg
    11701 acagatgcaa tcaggggtaa aagtagttgt tctgggaaat cttggtttca aagctgacaa
    11761 ttcttttttg catcatct
//
LOCUS       DASSHJ010000150        10136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1413647,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000150 DASSHJ010000000
VERSION     DASSHJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413647"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /gene="fliM"
                     /locus_tag="VFI43_05875"
     CDS             complement(<1..279)
                     /gene="fliM"
                     /locus_tag="VFI43_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441595.1"
                     /note="with FliG and FliN makes up the switch complex
                     which is involved in switching the direction of the
                     flagella rotation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HET7061689.1"
                     /translation="MTDDFLSPEEVDALLRGMARSSGRAQDDNAGSNCRPYNLATQER
                     IVRGRMPTLEIINERFARLLRLGLFNFMRRSANVTAGPVRVIKFSEFVR"
     gene            complement(284..784)
                     /gene="fliL"
                     /locus_tag="VFI43_05880"
     CDS             complement(284..784)
                     /gene="fliL"
                     /locus_tag="VFI43_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HET7061690.1"
                     /translation="MASRAIKQANGKKEGDTEQKSKLKWVIVIASTVLLLAAGWGGAA
                     WYFIQSKDSPAAPSKPKLPVFVNLETFTVNLQPEHSEQHLQTNLTLKVEDTAAVDAIK
                     LHMPEVRNRILLLLSNKTAGQLMTVEGKRKLAQELTVEINQQFAERKADGQVVESVLF
                     TSFVIQ"
     gene            complement(1034..2467)
                     /locus_tag="VFI43_05885"
     CDS             complement(1034..2467)
                     /locus_tag="VFI43_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HET7061691.1"
                     /translation="MTTPSIAGRPVTINPLSSKPSPTGSPGRMEDGAAELAFGKLLAS
                     EMGDECGISAGISIKKTPVARNIDRTDDAVNPEVAEAAGKSMKHDMPGTEDSVKGGAD
                     PNRDLFAQPFIQNLPPNLLSRAMRPALEAAVSAEAPEAMGTVFPGDVRIGKNAGPDVD
                     KDRVNLQDAGREGLRFDSIEIPGARGGTPANMVLQEDKAKAGMRPGVRASLNGPDINA
                     PMSGEASMLRGVFSDGHAEHREEAGSTRTAVVQNGIAVPMDVDARQGGEKRVEFAQDS
                     SFKEHIEKYIEKAGETEAVAGTQFLSVNPPHATAAASQPGLSMSQTTMAANHPYLLPH
                     VDSTGWSDALSQKVLWMATEGRQTAELSLNPPDLGPLQVVLSVDNDQASAMFVSQNPD
                     VRQALEAALPRLKEMMAESGISLGSTTVSADSSSQNSNPDGSGWGSVASGKSARGSLT
                     GTSMAREGGVMAIERNIERRGLVDTFA"
     gene            complement(2570..3007)
                     /gene="fliJ"
                     /locus_tag="VFI43_05890"
     CDS             complement(2570..3007)
                     /gene="fliJ"
                     /locus_tag="VFI43_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HET7061692.1"
                     /translation="MNTLIELARARSDDAARRLGKLNLQNMDLQAKLDLLLQYGEEYR
                     ARFDALMRQGLTASDWRNYREFLDRLDAAIVEQRAVLALMNQRVRAGQSAWKAARRTL
                     ASYDVLAQRQLQAERACAARSEQKQTDEHVNNATARQIQPPVK"
     gene            complement(3033..4460)
                     /gene="fliI"
                     /locus_tag="VFI43_05895"
     CDS             complement(3033..4460)
                     /gene="fliI"
                     /locus_tag="VFI43_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HET7061693.1"
                     /translation="MTTFPVSSHSSRWRSFLKNCSDTAVACSPFQLSGSLTRATGLVM
                     EAVGLRLAVGSGCTLMLPNGNRVEAEVVGFQGERLYLMPTSDVFGVTPGAKVIPWDDA
                     GTLPVGGNERNLRRRASDRARRFPVGDELLGRVLDGTGRPLDGLGVLKASRSAPLASR
                     PLHPLDREPLTQVLDVGVRAINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTSADV
                     IVVGLIGERGREVKEFIEQILGAEGLARSVVVAAPADTWPLMRLQGAFYATSIAEYFR
                     DQGRNVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFARLPQLVERAGNGVRGG
                     GSITAFYTVLTEGDDQQDPIADSARAILDGHVVLSRHLAESGHYPAIDIEASISRAMS
                     ALISPEYLERAREFKRLYSSFQRSRDLISVGAYSSGSDPLLDRAITLYPRLESFLQQS
                     MFERTDYSDSAARLQGLLASALMEH"
     gene            complement(4457..5182)
                     /locus_tag="VFI43_05900"
     CDS             complement(4457..5182)
                     /locus_tag="VFI43_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HET7061694.1"
                     /translation="MSRRIIPKEKLSSCRSWEMEAFERRRSLPPDDEHDRQCDATGED
                     RRDQGTLGEKSEAEHDEPSSAEGYGHGYEAGQKAGYEAGYGEGRNSGYQAGYEAGMER
                     AVDEAKRLEALLQEFQRELSTADQVIGQDLLSLALSLAKQMAREALRIKPEIVCTVVR
                     ECLRHEPGFNQPKQLFLNVEDAELVRKYLDQELEGWVICVEPQIERGGCRIKMGHGEI
                     DATTETRWLRIAQSLGQSSAWME"
     gene            complement(5233..6231)
                     /gene="fliG"
                     /locus_tag="VFI43_05905"
     CDS             complement(5233..6231)
                     /gene="fliG"
                     /locus_tag="VFI43_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HET7061695.1"
                     /translation="MNEEGINKGAILLLSLGEDEAVQVLKHLNHLEVQKLGAAMAKLK
                     NVSREQVDGVLSDFCSRAVGEPRFLDLDPENYARTVLQRALGEDKSTDNISRILEGHD
                     TSGIEGLKWIDSQSVAELIKGEHPQIIATILVHLDRNQASEILGELSEQLRNDVLLRI
                     ATLEGIQPSALRELNTVLTDLLSGSENINKSSVGGIRTAAEIINFMGSTAENSVLDKL
                     REFDGELAQKIIDQMIVFENLMDIDDRGIQVLLREIQSDALVIAMKGAAPDLREKIFR
                     NMSSRAAEMLREDLEVMGPVRVSEVEAQQKQILQVVRRLADEGQIVFGSKNAESYV"
     gene            complement(6228..7889)
                     /gene="fliF"
                     /locus_tag="VFI43_05910"
     CDS             complement(6228..7889)
                     /gene="fliF"
                     /locus_tag="VFI43_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HET7061696.1"
                     /translation="MAVDTETVTISGSQGFMRSFLGRISSQQKIVLMLASSTIIALAA
                     GGWMWGKTPDYKVLFSNISDRDGGAIVVALQQMNVPYKFAEGGAAILVPDSQVHDVRL
                     KLASQGLPKGGLVGFELMENQKFGTSQFLEQVNFNRAVEGELARSIQSLSAVSSARVH
                     LAVPKQSVFMREQPKPSASVLVNLHPGRTLDAQQVSAVAHLVSSSVPNLPLKNVTVVD
                     QHGNLLSHTDDPSTNNGLDPGQLKYMHAVERSYIERIEAILSPIVGRENVRAQVTADI
                     DFAQSEHTSEMYKPNQNPGDSAVRSQQTSESSTRPGAGEGGVPGALSNQPPAPATAPV
                     TTPPGTPAPAPDSAPPPAPPNTRKDATINYEVDKTIQHTRKPVGSIKRMSVAVVVNYR
                     KITDQNGASSTKPLTEAEKAQITDLVKEAMGFNNERGDTLNVVNSPFADIEREALPEA
                     PLWKQPEMIDLAQQVGKMLLIGGLILYVILGVLRPTLKRINHPPAPVLAAPTNESHSE
                     VIPAAVMPSPPLISQHEQNLAMARQLAKDDPKRVANVVKQWVIGE"
     gene            8082..8459
                     /gene="fliE"
                     /locus_tag="VFI43_05915"
     CDS             8082..8459
                     /gene="fliE"
                     /locus_tag="VFI43_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HET7061697.1"
                     /translation="MITCVPYPKTGEVFMDVSKIDVMLAQLRTGAALAGSKVSPRDSG
                     EGMEGSSGVDFSAALRNSLDSVNAMQQGAAKLARSFELGEPEANLTEAMIAMQKASLS
                     FQYTIQVRNKLVAAYQEIMNMPV"
     gene            complement(8535..>10136)
                     /locus_tag="VFI43_05920"
     CDS             complement(8535..>10136)
                     /locus_tag="VFI43_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061698.1"
                     /translation="GFHAAGMTFEKYDKVGIDVRAYEHFVAMWTSLFSAASNKERILT
                     EWHASYVDARNEGSAGTLEPNGIYYTGKTLFQPVPANLSIRHYFLLDEPDRSRELAAL
                     IRRLQRMDVKVYRLDKPLSVPDFRRYGEVPSLVTLPSGTYWVPMAQAQKHWIQAMLHE
                     NPYIPVTVSYDVSAWSNPLLMNIAGGSSGAALKPSAGLVPPVAEPALPGPPPGAPRIG
                     LFQIPGSSGITSAGSIRYLFQRVWGVPFTEVDASQIQAGLSGVDVLLVPDGYASYGLQ
                     ALGSKGQRALADWVAAGGKYVGYVGGAELAVKAGVSTAVLKPSNTSAPGALIRAKLDE
                     GSILAQGVGPAAWVMYDDNNLMTPGLGEAAATFPAAGDPYYHTSGLAIGVDELAGTAA
                     VVDEAVGAGRSIVFSFDPNFRAWSDGTQRVLWNALFAPEPVAAAAAPAKVRASAVDRA
                     GKAAALLPDMGKAIRIVVHSSDADTARALIQRYGAEFKESGKSDRTIFLIENRKGLSR
                     EEHPFIIDLARELGNQITPISISVP"
BASE COUNT         2050 a         3030 c         2732 g         2324 t
ORIGIN      
        1 gcgcacgaac tcgctgaact tgatcacgcg caccggcccc gcagtcacat ttgcgctgcg
       61 gcgcatgaag ttgaacaatc cgaggcggag aaggcgcgca aaacgctcat tgatgatctc
      121 gagcgtgggc atgcgcccgc ggacaatacg ttcctgagtg gcaagattgt acggacgaca
      181 attactgccg gcgttgtcgt cctgcgccct gccagacgat ctcgccattc ccctgagcag
      241 cgcatcgacc tcttccgggg atagaaagtc atccgtcatg cgcttactgg atcacaaacg
      301 aagtaaagag gacgctttcg accacctgcc cgtccgcttt cctttcggcg aattgctggt
      361 tgatctcgac tgtcaattcc tgcgcaagct tccgcttgcc ttccacggtc atcaactgtc
      421 ccgcagtctt gtttgaaagc aacagcagga tacgattacg cacttcgggc atatggagct
      481 tgatggcatc tacggctgcg gtgtcttcaa ccttcaacgt caaattggtc tgaagatgtt
      541 gttcgctatg ctccggctgg aggttgacgg taaacgtttc caggttgacg aaaacaggca
      601 gctttggctt tgaaggcgcc gctgggctat ccttggactg gataaagtac cacgctgccc
      661 ctccccatcc ggctgccagc aacagcactg tggaggcaat gacgataacc catttgagtt
      721 ttgacttttg ctcagtgtcg ccttctttct tgccgtttgc ctgtttgatc gctctgctcg
      781 ccatgatttt taagtagcgg aagacgtgca attattgcag cacgagctct tcagtcatgc
      841 ggggaacagg gattgaaagg gatgttatct tgggggttga gcaggaggct tttcccgcgt
      901 gtccaaggcg tatggaagat tccaatcatc gaggctggga tccgcacacg accacctcct
      961 gcctggtcgt ttcctgctct ggtctgagtc tgggctgtta acaggccctg gcatccctgt
     1021 ccaggccgca acctcaagca aaagtatcaa ccaatccgcg ccgctcgata ttccgctcga
     1081 tggccataac tcctccctcc cgagccatgg acgttccggt caagcttccc ctcgcgcttt
     1141 tgccgctggc aacgctaccc catccagacc catctggatt gctgttctgg ctggagctat
     1201 cagcactgac cgtcgtgcta cccaaactga tgccgctttc cgccatcatt tccttcaagc
     1261 ggggcagcgc agcttcgagc gcctgccgca cgtccgggtt ctgggaaaca aacattgcac
     1321 ttgcctggtc gttgtcgaca gatagcacga cctgcaaagg gccgaggtca ggcgggttaa
     1381 ggctcaattc cgccgtctga cggccctcgg tggccatcca caacactttt tggcttaaag
     1441 catcactcca tccagtggaa tcgacatgcg gaagcaggta tggatgattc gcagccattg
     1501 tagtctgaga catcgacaaa ccgggctgcg atgctgccgc cgtggcgtga ggtggattaa
     1561 cggataaaaa ctgagttccg gcaacggctt ctgtctcacc cgctttttcg atgtattttt
     1621 caatatgttc tttaaagctg gagtcctggg cgaattccac ccgcttctcg ccgccctgcc
     1681 tggcatccac atccatcggg accgcaatac cgttttgaac aaccgcggta cgggttgagc
     1741 cggcctcctc ccggtgttca gcatgcccat cggaaaacac gccgcgaagc atgcttgctt
     1801 ctcctgacat cggagcattg atatctgggc cgttcaagga ggcgcgaact cccggcctca
     1861 ttcccgcttt cgctttgtct tcctgcaaaa ccatattggc tggagtacca ccccttgctc
     1921 ccgggatttc aattgaatcg aaccgtaatc cctcccttcc ggcatcttga aggttaaccc
     1981 tgtctttatc gacatctggt ccggcatttt ttccaattcg gacatcgccc gggaaaacgg
     2041 tccccattgc ttccggggcc tccgctgaca ccgctgcctc gagtgccggt cgcattgctc
     2101 gcgagaggag attgggtggt agattttgta tgaacggttg tgcgaagaga tcacggtttg
     2161 gatccgctcc gcctttgacg ctatcttccg tacccggcat atcgtgcttc atgctttttc
     2221 cagccgcctc ggcaacttct ggattcaccg catcatcagt gcgatcgatg tttctggcga
     2281 caggtgtttt ttttatgctg atacccgctg atatcccaca ttcgtcaccc atttcgctcg
     2341 ccagcagttt tccgaaggct agctcagccg ctccatcctc cattctgccc ggactacccg
     2401 tcggtgacgg cttgctggaa agaggattta tggtaacggg acggcctgca atggatggag
     2461 tagtcattat gtgctctaag agtgacatct aaggatggca agggctggtt tatgccaggc
     2521 ttggccgtct ggccagttca tcaagagatg gacgccattg atcgtttaat catttgactg
     2581 gcggctgaat ctgccttgca gtggcattgt tgacgtgctc gtcagtctgt ttctgctcgg
     2641 atcttgccgc acacgctcgt tcagcctgta attgccgttg cgccagcaca tcgtatgatg
     2701 caagcgtccg ccgcgccgct ttccaagcgg actgtcccgc cctcacacgt tgattcatta
     2761 gcgccagaac cgctcgctgc tccacgatgg cggcatcgag cctgtcgaga aattcccgat
     2821 aattccgcca gtccgatgcg gtgagcccct ggcgcatcaa ggcgtcgaag cgcgctcggt
     2881 actcttcgcc atattgaagc aataaatcaa gttttgcctg taaatccatg ttttgaaggt
     2941 taagtttccc caatcgccgt gctgcatcat cgctgcgggc ccgcgcaagc tctatcaagg
     3001 tattcaaggc agagattctg gatgtcaacc gttcaatgct ccatgagtgc cgatgcgagc
     3061 aagccctgca gccgcgcggc gctatcggag taatccgtcc gctcaaacat gctttgctgt
     3121 agaaacgact cgagccgcgg gtacaaggtt atagcgcggt ccagcaaggg atcgctgccg
     3181 gacgaatagg ccccgacgct aatcaggtcg cgcgagcgct ggaaagacga atacaggcgc
     3241 ttgaactccc tggccctctc aagatattcg ggcgaaatca gagcgctcat cgcgcgactg
     3301 atcgacgctt cgatatcgat ggcgggataa tggccgcttt cagcgagatg acgagacagc
     3361 acgacatgcc cgtcgaggat tgcgcgggcc gaatcagcga tcggatcctg ctggtcgtca
     3421 ccttcggtca gaaccgtata gaacgcagta atcgaaccgc ctcccctgac gccattgccc
     3481 gctcgctcca cgagctgcgg cagtctggcg aacacggaag gggggtaacc cttcgttgcg
     3541 ggcggctctc caatcgccag cgcgatctcg cgctgggcca ttgcgtagcg ggtcagcgaa
     3601 tccatgatca ggagaacgtt cctgccctga tcacggaaat actccgcgat ggaagtggcg
     3661 taaaaagccc cttgcaatct catcaaaggc caggtatcag cgggcgctgc aaccactacg
     3721 gaacgggcta gcccctcggc gccaagaatc tgctcgatga actctttcac ttcccggccg
     3781 cgctccccga tcaaaccgac cacgatcacg tcggcactcg tgtagcgcgc catcatgccg
     3841 agcaatacgc ttttaccaac ccccgaaccc gcaaagagac ccatccgttg cccgcgtccc
     3901 acggtcagca gcgcattgat ggctcgtacg ccgacgtcca ggacctgagt aaggggttcg
     3961 cggtcgaggg ggtgcagggg tcgtgatgca agcggagcgc tgcggctggc tttcaacact
     4021 cccaaaccat ccagtggacg cccggttcca tccagcactc gcccgagcaa ttcatcgccc
     4081 accggaaagc gtctggcgcg gtctgacgcc cggcgacgca aattgcgttc attgccccct
     4141 actggtaatg tcccggcgtc atcccacggg atcacttttg cgccgggagt gacgccgaat
     4201 acatcgcttg taggcatgag atacagccgt tccccctgga aaccgacaac ctctgcctca
     4261 acccggttgc cgttgggtaa catcagcgtg caaccgctgc ccacggcaag cctgagaccc
     4321 actgcttcca tcacaagtcc tgtcgcgcgg gtgagactgc ccgacaactg aaagggtgag
     4381 caggcaaccg ccgtatccga acagtttttg aggaagctgc gccatcgaga ggaatggctg
     4441 gatacaggga aggtcgtcat tccatccatg cgctgctttg tcccagtgac tgtgcaatac
     4501 gcagccagcg ggtctcggtc gttgcatcga tctcgccatg ccccatcttg atccggcatc
     4561 cacctcgttc gatctgcggt tcaacacaga taacccaccc ctccagctcc tgatccagat
     4621 atttacgcac cagctccgcg tcctccacat tcaggaataa ttgcttgggc tggttgaacc
     4681 cgggctcatg gcgaaggcat tcgcgaacaa ccgtgcagac aatctccggt tttattcgca
     4741 atgcctcgcg agccatttgc ttggccagac tcagggcaag cgacagcaga tcctggccga
     4801 tgacttggtc ggctgtggat aattccctct ggaactcctg caacagggcc tccaaccgct
     4861 tggcctcatc cacagcgcgt tccattccgg cctcgtaacc cgcttggtaa ccggaattcc
     4921 ggccctcccc ataaccggcc tcgtaaccgg ctttttggcc cgcctcgtat ccgtgcccat
     4981 aaccttccgc ggaggaaggt tcatcatgct cggcttcgct tttctccccg agcgtcccct
     5041 gatctcgccg gtcctctcct gtcgcgtcgc actgtcggtc atgctcgtca tcagggggta
     5101 gggagcgccg ccgctcgaat gcctccatct cccagctgcg gcaggaagag agtttttcct
     5161 ttggaataat tcgtctagac atgaccactc acgccactcc cggaaataat gcatggattt
     5221 ctttcgtttt tttcaaacat aactttccgc gttctttgac ccgaatacga tttggccttc
     5281 atccgcaagc cgccggacaa cttgcagaat ctgtttttgt tgagcttcga cttcgctcac
     5341 tctgacgggt cccatgactt ccagatcttc cctcagcatc tcggctgcac ggctcgacat
     5401 attcctgaaa atcttttctc tcagatccgg ggcggcgcct ttcatcgcaa tcacaagcgc
     5461 gtcggactga atttccctca gcagtacctg tatcccacgg tcgtcgatat ccatcaaatt
     5521 ttcgaatacg atcatctgat ctatgatttt ttgcgccagc tcgccatcga attcgcgtaa
     5581 cttgtccagt acggaattct cggccgtgct tcccatgaaa ttaatgattt ccgccgctgt
     5641 ccggatgcct cccaccgagc tcttgttgat gttctcgctc ccggacaaca gatcggtcag
     5701 aaccgtgttc aattcgcgga gtgcggaagg ctggatgcct tcaagagtgg caatgcgtag
     5761 taaaacgtca tttcgcagtt gctcggaaag ctcgccgagg atttcgctcg cctgattgcg
     5821 atcgaggtgt accagtatgg tcgcaatgat ctgagggtgc tcccccttta tcaactccgc
     5881 gaccgattgc gagtcgatcc atttgagtcc ctcgattccg ctcgtatcgt gcccttcgag
     5941 gatacggctg atgttgtcgg ttgacttatc ctctcccaga gcccgctgaa gcacagtgcg
     6001 cgcgtaattt tcgggatcca gatccagaaa acgcggttcg cccactgccc ggctgcaaaa
     6061 atcgctcagc acgccatcga cctgctcgcg gctcacattt tttaacttgg ccatcgctgc
     6121 gccgagcttc tgcacttcaa ggtgattgag atgcttgagc acctgcacgg cttcgtcctc
     6181 gcccaaggaa aggagcaata tagccccctt gtttatgccc tcctcgttca ttcccctatc
     6241 acccattgct tcactacatt cgccacccgc ttcgggtcat cctttgccaa ttgccgggcc
     6301 atcgcgagat tctgttcgtg ctgggaaatc agcggagggc tcggcataac ggcggcggga
     6361 atcacttcgg aatgagattc attcgtcggg gcggccaaca cgggagcagg gggatgattg
     6421 atgcgcttga gtgtcggtct gagcacgcca aggatgacat agaggatcag acctccgatg
     6481 aggagcatct ttcccacctg ctgggcaaga tcgatcattt cgggctgttt ccatagggga
     6541 gcctcgggta aggcttcgcg ttcgatgtcg gcgaaggggc tattgacaac gttcagcgtg
     6601 tctcctcgct cgttgttgaa tcccatcgcc tccttcacca ggtccgtgat ctgcgccttc
     6661 tcagcctcgg tcaatggttt cgtgctggac gcgccattct gatcggtaat cttgcggtaa
     6721 ttgacgacta ccgccaccga catgcgcttg atgcttccga cgggttttcg cgtgtgctgg
     6781 atcgtcttgt caacctcata gttgatcgtc gcatccttgc gggtgtttgg aggcgcaggc
     6841 ggtggagctg agtccggggc cggggccggt gtaccgggcg gggtcgtgac cggagcggtt
     6901 gccggcgctg gaggctggtt tgaaagcgcc cccggaaccc ctccctcacc ggctccgggt
     6961 cgagtagatg attcggacgt ttgctggctg cggacggccg aatctccggg attctggtta
     7021 ggcttgtaca tttcgctggt atgctcggac tgagcgaaat cgatatccgc tgttacctgc
     7081 gcgcggacat tctcacgacc gacaatgggg gagagaattg cctcgattcg ctcaatgtag
     7141 cttcgctcta ccgcgtgcat gtatttaagc tgaccaggat caagcccgtt attggtgctc
     7201 ggatcatccg tatggctcag taggttgccg tgctgatcca ccacggtgac attcttcagc
     7261 ggcagattgg gaacgctgct ggaaacgaga tgcgcgaccg cgctgacctg ctgagcgtcg
     7321 agcgtgcgcc cagggtgtag attgacgagt accgaagcgc tgggttttgg ctgttcacgc
     7381 atgaacacgg attgcttcgg aaccgccaga tggacacgcg cactgctgac cgccgatagc
     7441 gattgaatcg aacgcgcaag ctctccttcc accgcgcggt tgaaattgac ctgctcgaga
     7501 aactggctgg tcccgaactt ctggttttcc atcagctcga aaccgaccag cccccccttt
     7561 ggcaaaccct gcgatgccag cttcaggcgt acatcatgca cctggctgtc aggtacgaga
     7621 attgctgccc cgccctccgc aaacttgtag ggcacgttca tctgctggag cgccacgacg
     7681 atcgctccgc catcgcggtc ggaaatattg ctgaaaagca ccttgtaatc gggtgtcttg
     7741 ccccacatcc agccgccggc ggcgagcgcg ataatagtgg atgaggccag catcaacact
     7801 attttctgct ggcttgagat cctgcccagg aagcttcgca tgaagccctg cgatccgctt
     7861 atcgttacgg tttcagtatc aaccgccacg cactgacctc caatgcttta ttctatgcgc
     7921 cacgttttta ttggttttcg tatctggatt cagcccgatt ccatggccga agcagcgggt
     7981 gctgacggca tagatcagca ttatcagcag gtacggttat ttcgatgtgg cgaatagcaa
     8041 cgggtttagg aggttattca cgcaagaacc ggaaattggc tgtgataact tgtgtaccgt
     8101 acccaaagac cggtgaggtt ttcatggatg tgtcgaaaat agacgtgatg ctcgctcaat
     8161 taagaacggg cgcagcactt gccggtagca aagttagccc gagagactcc ggtgagggta
     8221 tggaaggatc aagcggcgtc gatttttccg cggccctcag gaattccctc gacagcgtca
     8281 acgccatgca gcaaggcgcg gcgaagctcg cccgttcttt cgagctgggc gaaccggaag
     8341 cgaatctgac cgaggcaatg atcgcgatgc aaaaggcgtc gctctcgttt caatacacga
     8401 ttcaagtgcg caacaagctg gtcgccgcct accaggaaat catgaatatg cctgtgtaga
     8461 tgaagaaggc ctatcgaaga aagcctgttc gcccgtttct ctgcttcttc aaaccgcgca
     8521 tgtcgcagtg ccccctatgg aacgcttatc gagattggag tgatctggtt cccgagttca
     8581 cgagccagat caatgataaa ggggtgctct tccctcgaaa gtcctttacg gttctcaata
     8641 agaaaaatcg tacgatcgga cttccctgat tccttgaact ccgccccgta acgttgaatc
     8701 agcgcccgtg ctgtgtctgc atccgacgaa tgcacgacga tccgaatcgc cttccccatg
     8761 tcaggcaata aggcagccgc tttgcccgca cggtcgaccg ctgaggctct caccttcgcc
     8821 ggtgcagccg cggctgcaac cggttcggga gcgaacagag cgttccaaag cactctttgc
     8881 gtaccgtcgg accaggcgcg aaaattcgga tcgaaggaaa atacgatgct gcgccctgca
     8941 ccaacggctt catccacaac cgcggctgtg cccgccagtt cgtccacccc gatcgccagt
     9001 ccggaggtgt gatagtaagg gtctccggcg gccggaaacg ttgccgcagc ctcgccaagg
     9061 ccgggggtca tgagattgtt gtcgtcatac atcacccagg ccgcgggacc cacaccctgt
     9121 gcaaggatgc ttccctcatc gagcttggct cggatgagag ctcccggtgc gcttgtgttg
     9181 gaaggcttca agactgcggt ggagactcct gccttgacag ccagttcggc gccaccgaca
     9241 tagcccacat atttgcctcc ggcggcaacc caatcggcca gcgcccgctg tccttttgat
     9301 cccagcgcct gcaacccata gctggcatat ccatcgggaa caagcagtac gtcaacccct
     9361 gacagccctg cctgtatctg gctggcatcc acctcggtaa aaggaactcc ccagacacgc
     9421 tggaacagat aacggatcga gccagcggaa gtaataccgg aagaacccgg gatctggaac
     9481 agtccgattc ttggcgctcc cggtggcggc cccggcagcg cgggttcagc aacgggcggc
     9541 acaagtcctg ccgagggttt cagcgcggcg cctgatgagc cgccggcaat attcatgagc
     9601 aagggattgc tccacgccga gacatcgtaa ctcacggtga ccggaatgta cggattctca
     9661 tggagcattg cctggatcca gtgtttctgc gcctgcgcca tgggtaccca ataagtgccg
     9721 gatggtagcg tgacgaggga cggcacttcg ccatagcggc gaaaatccgg cacggaaagc
     9781 ggcttgtcga gacggtagac cttgacgtcc atacgttgca agcggcggat aagggctgca
     9841 agctcgcgcg atcgatcggg ctcatccagg agaaaataat gccggatgct gaggtttgca
     9901 gggacgggct gaaacagcgt cttgcccgtg taatagattc catttggctc gagtgtcccg
     9961 gcgctgccct catttcttgc atcaacgtag gatgcatgcc actccgtcag gatgcgttcc
    10021 ttgttgctcg ctgctgaaaa aagcgacgtc cacatcgcca cgaaatgttc atacgcgcgc
    10081 acgtcgatgc ccaccttgtc gtatttctcg aatgtcatgc cagcggcatg aaaacc
//
LOCUS       DASSHJ010000151         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_4772, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000151 DASSHJ010000000
VERSION     DASSHJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4772"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFI43_05925"
     CDS             <1..388
                     /locus_tag="VFI43_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7061699.1"
                     /translation="SGGELSRIGLAIHVITSKLGAAPTLIFDEVDVGIGGRVAEIVGE
                     MLKKLSGQRQILCITHLAQVASAADNQWLITKTAGSSGDGAISSRITALNKEERIEEI
                     ARMLGGVKITETTRRHAAEMLFASQK"
     gene            complement(493..1071)
                     /gene="folE"
                     /locus_tag="VFI43_05930"
     CDS             complement(493..1071)
                     /gene="folE"
                     /locus_tag="VFI43_05930"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7061700.1"
                     /translation="MDSSNDSEFGLADWQRLLRSIGEDPFRQGLIETPGRVSRAWAHW
                     TRGYKQDPAAILKTFADGGEHYDELIVVRQIPVYSHCEHHLAPFFGHATVGYLPSGHI
                     VGLSKLTRLVNCFAARLQVQERLTQQVAQSLLEHLQPKAVGVIIRCRHMCMESRGIAV
                     AGEETVTSAMLGDLKTNAAQRAEFLALAERPD"
     gene            1255..2025
                     /gene="xth"
                     /locus_tag="VFI43_05935"
     CDS             1255..2025
                     /gene="xth"
                     /locus_tag="VFI43_05935"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7061701.1"
                     /translation="MKIATWNVNSLKVRLPHVLDWLATHQPDVLCLQETKLQDEHFPA
                     AEIEAAGYQTLYTGQRTYNGVALLSKRPATEHIMGIPDLDDHQKRVVSAMYGDIRVIC
                     VYVPNGQKVGSEKYEYKLRWLAALKNWIREMLARHQKLAVLGDFNIAPEERDVHDAKL
                     WEGQVLFSLPEREAFKEVLNLGLVDSFRLFEQADKSYTWWDYRMMAFRRNMGLRIDHI
                     LLSDELARTCTACAIDKMPRKLERPSDHAPIMVELGCM"
     gene            complement(2105..2821)
                     /locus_tag="VFI43_05940"
     CDS             complement(2105..2821)
                     /locus_tag="VFI43_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HET7061702.1"
                     /translation="MESLLNCTTVEELHSNATAIARQIGFEHFLYGVQVNTSLTRPYR
                     FILSCYPEEWRTHYDKEGYASVDPTVSHCAKTSIPIIWKNEIYKGRKENQFRNEAKDS
                     GLVSGVSFPVHGSRGEVAMLSLSTSRESREAENDILAVMGKAQLLTCYLHEAVQRIVL
                     SKGPVPLKKINLTEREKECLLWAAEGKTGWEIANIISISERTVTFHLQNAAQKLGVVN
                     RQQAISRALSMGLIEPQAIV"
BASE COUNT          682 a          722 c          792 g          627 t
ORIGIN      
        1 atccggagga gaactgtctc gtattggcct ggccatccac gtgatcacga gcaagcttgg
       61 cgcagcaccc accctgatat ttgatgaggt tgacgtcggg atcggcgggc gagtggcgga
      121 gattgtcggt gaaatgctta aaaaactgag cgggcagcgc cagatcctat gcatcaccca
      181 tctcgcccaa gttgcttcag cagcagacaa tcagtggttg attacgaaaa cagccggttc
      241 gtcgggcgat ggcgcgatat cgagccggat taccgccctc aacaaggagg agcggatcga
      301 ggaaattgca cgcatgctag gcggggtaaa aatcacagaa acgacgcgca ggcatgcggc
      361 ggaaatgcta tttgcaagcc agaagtagcg gatggaggaa ccgttctttt tcgagactct
      421 gcttgcggga agagccggag aaagcaagcg tattgcggaa tctgtaggca ggtatattgc
      481 gggaagagtc gcctaatcgg gccgttcggc cagggccaga aactcggcgc gctgcgctgc
      541 gttcgtcttg agatcgccca gcatcgcgga tgtaacggtt tcctcgcccg caaccgcaat
      601 ccctctcgac tccatgcaca tatggcggca gcggataatt acgccgaccg ccttgggttg
      661 gagatgctca agcagggact gggctacttg ttgggtcaaa cgttcctgca cctgcaacct
      721 tgctgcaaaa cagttgacga ggcgggtcag cttggaaagt ccgacaatat gcccgctcgg
      781 aaggtagcca acagtagcgt gcccaaagaa gggggccagg tgatgctcgc aatgcgaata
      841 gactggtatc tgcctgacaa cgatcagttc atcgtagtgc tcgcccccat ccgcgaaggt
      901 cttcagaatg gcagcaggat cctgcttgta gccgcgggtc caatgagccc acgcccggct
      961 gacacgccca ggggtttcga tcagcccctg gcggaaagga tcttctccta tggaccgtaa
     1021 cagtctttgc cagtcagcta ggccaaactc cgaatcattc gagctatcca taatcaattc
     1081 gcctttggtt gaaggagaag cggtaaaacg ttgccggaca ttcgcgagtc ggcggggacg
     1141 cgttagagag ctgtttcata atagcataac cgtgctgggc caagggaatg cgattaaaga
     1201 gcatgcaaaa tcatggggat ttattccgag aacttgtaac tggtaaaatg catcatgaag
     1261 atcgcaacgt ggaatgtaaa ttctctcaaa gttcgcctcc cgcacgttct cgactggcta
     1321 gcgacgcacc aacctgatgt gctatgcctg caggaaacaa aattgcagga tgaacatttc
     1381 cccgcagccg agattgaagc ggctggttat caaacgctct atacagggca aagaacttat
     1441 aacggggtgg cactgctcag caagcgcccg gcaactgagc acatcatggg catccccgat
     1501 cttgatgacc atcagaagcg cgtggtttcg gcgatgtatg gcgatatcag ggtaatttgc
     1561 gtctacgtgc cgaacggcca gaaagtagga tcggagaagt acgaatataa gttgaggtgg
     1621 ttggcagccc ttaagaactg gatccgggag atgttggcaa ggcaccagaa actggcagtg
     1681 ttgggcgatt tcaatatcgc gcctgaggag cgcgacgtac atgacgcaaa gttgtgggaa
     1741 ggtcaggtcc tgttcagtct gccggagcgc gaagccttca aggaggtgct caacctcggc
     1801 ctggtggaca gcttccgtct gtttgagcaa gcggataaat catatacctg gtgggattac
     1861 cgcatgatgg cgtttcgccg caacatgggt ttgagaatcg accacatctt gctgagcgac
     1921 gaacttgcac gaacatgtac ggcttgtgca atcgacaaga tgccgcgcaa gctcgagcgg
     1981 ccttccgatc atgcccccat aatggttgaa cttggctgca tgtaaatacc tatcgaagaa
     2041 tacctgccag agggaaaaag tgaacgcgta caacagcctg tattccggcg acggaaccat
     2101 ccgcttaaac gatggcctgc ggttcgatca ggcccattga taaggcgcga gagatcgctt
     2161 gttgccgatt gaccaccccg agtttttgcg ctgcgttctg gagatggaat gttaccgttc
     2221 gttcggagat actaatgatg tttgctattt cccagcccgt cttgccttcc gccgcccata
     2281 gcaggcattc cttttcgcgt tcggtcaaat tgattttctt caacggtacc gggcctttgc
     2341 tgagaacaat gcgctgcacg gcttcatgca ggtaacacgt caatagctgc gcctttccca
     2401 tcacagccag aatatcattc tcggcttcac gcgattcgcg ggacgtggag aggctcagca
     2461 tggccacttc gccacggctg ccatggactg gaaaactgac tccacttacc aggcctgaat
     2521 ctttcgcctc gtttctgaac tggttttcct tacgcccctt gtaaatttca ttcttccaga
     2581 taataggaat cgaagtttta gcacagtgcg aaacggttgg atcaacactg gcatagcctt
     2641 ccttgtcgta gtgcgtgcgc cactcctctg gataacagct caggatgaac cggtaaggac
     2701 gggtaagcga agtattgact tgtaccccat agagaaaatg ctcaaatccg atttgtcggg
     2761 ctatagcggt cgcgttgcta tggagttcct ccacagtggt gcagttcagc agagattcca
     2821 ata
//
LOCUS       DASSHJ010000152        16651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_121273, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000152 DASSHJ010000000
VERSION     DASSHJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 16651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16651
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121273"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2244)
                     /gene="polA"
                     /locus_tag="VFI43_05945"
     CDS             complement(<1..2244)
                     /gene="polA"
                     /locus_tag="VFI43_05945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7061703.1"
                     /translation="MKTLLLVDGSSYLYRAFHALPDLRNRNNEPTGAIYGVLNMLRRL
                     HKDYQADYSACVFDAKGKTFRDDLYPEYKSHRPPMPGDLVAQLEPLYECIAAMGWPML
                     VIEGVEADDVIGTLVKKAEPENLRCIVSTGDKDIAQLVKPGVTLINTMTNEVLDEAGV
                     RARFGVPPERILDYLALVGDATDNIPGVEKVGPKTAVKWLTQYGTLDNLIARADEIGG
                     VVGENLRKALGWLRKSPEILAIKCDVPLPVDLHELGPQPQNTSKLLELYDRLDFKTWL
                     RELRDQESGGEYQESTSSSGGPPRKPDVPPVDYQAVLTESELDQWIARLNDAPLVSLD
                     TETTSLDPMKARLVGIAFSVKCHCAAYIPLAHGYAGAPPQLPLDLVLHKLKPWLENRE
                     KLKLGQNLKYDKHVFANHGIELNGIVHDTLLQSYVLEPHRAHDMDNLALRHLDIRTVT
                     YDDVTGKGASRIGFDQVEIERATRYAAEDADITLCLHEHLYPQMSADVSLSYVYRELE
                     MPLLEILFEMERNGVLLDLKMLEIQSRELAEKMLMLEEHACAVAGLPFNLNSTKQVQE
                     VLFTRLKLPVLKKTPGGTPSTDEDVLQRLALDYPLAKMLLDYRGLAKLRSTYTEKLPR
                     MVDTTTGRVHTNYAQAVAVTGRLASNEPNLQNIPVRTAEGRRIREAFIAPPGYRIVSA
                     DYSQIELRIMAHISHDEGLLRAFGAGEDIHRATAAEVFGIPREAVSSEQRRYAKVINF
                     GLIYGMSE"
     gene            2264..2992
                     /locus_tag="VFI43_05950"
     CDS             2264..2992
                     /locus_tag="VFI43_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7061704.1"
                     /translation="MNSQEKPEHPLPSDYPEKARNATESWRIFGIMAEFVEATERLNS
                     IRPAVSIFGSARTSPDHPYYKLTEQIANQLSEAGFSVISGGGPGIMEAANKGAYLGPS
                     PSVGLNIQLPHEQHRNVYQDISQTFRHFFARKYMFVRFATAYVVVPGGFGTLDELMEA
                     LTLVQTGKTRKMPIILVCSAFWQGMLDWLRQTLVGEGMISPEDMDLIQIIDEPGEVVK
                     AIFRYYESRGFEPSAAEREVQLNL"
     gene            3047..3409
                     /locus_tag="VFI43_05955"
     CDS             3047..3409
                     /locus_tag="VFI43_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HET7061705.1"
                     /translation="MRQIAVALLLSVALPASAQSNQPKDLRPLPEVPEPPELALPAPG
                     ADQEDEALEPQVTITRRGEDQVEEYRLNGRLYMIKVIPRIGLPYYLIDNYGDGTFATG
                     TGGLDQRIRPPMWVIRQF"
     gene            3625..4590
                     /locus_tag="VFI43_05960"
     CDS             3625..4590
                     /locus_tag="VFI43_05960"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7061706.1"
                     /translation="MSVFTTVAPHQLSLWLKNYPLGTLLDLQGISSGIENTNYYVTTS
                     LGKYILTLFEKLTAGDLPYYLNLMAYLSHQSIPCPRPIPSLNNELLGTLNGRPASIVT
                     CLPGKSRENPSTLDCAKVGKLLADMHLRGLSYPGEMQNSRGPRWWKAAAHAVMPFLTR
                     EEADVLKEELHFQSLYRFEDLPRGVIHADLFRDNVLFDGGAVGGVIDFYFACNDVLLY
                     DLAITANDWCLTSNGKLDPDRTFSMLEAYHACRPLTSTERRAWPVMLRAAALRFWLSR
                     LHDYHLPRVGELTHVKDPAQFMRILANHAASHDELAKIWVEHTRN"
     gene            4602..5360
                     /locus_tag="VFI43_05965"
     CDS             4602..5360
                     /locus_tag="VFI43_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HET7061707.1"
                     /translation="MSRGESPPEIGNMDVRQVDGKQGWQWIVSGFLLFRRFPMVWILL
                     CTTLLLIAATLALIPVAGQFIFTLLSPVFLAGLMIGCRTLEKGGQLEIAHLLAGFRYG
                     ATPLITIGGIYLIGQVLIFGIAMLIGGETMRDLLIAGRRVDENELQEVMSSSLSALLV
                     ALTLSIPLMMATWFAPLLVIFRNMPPLEAMRLSFFACIKNIVAFQIYTFFLVVLTIVA
                     TIPYGLGLFVLVPTLFASIYASYEDIFSETPGVA"
     gene            complement(5401..7623)
                     /locus_tag="VFI43_05970"
     CDS             complement(5401..7623)
                     /locus_tag="VFI43_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7061708.1"
                     /translation="MSSLLSALNPEQLEAVTLPHQSVLVLAGAGSGKTRVLTTRIAHL
                     IQSGQVSPHGILAVTFTNKAAKEMLMRISSMLPVNTRGMWVGTFHGLCNRMLRAHHQD
                     VGLPQSFQILDTADQLAMIKRILKNLGVDDQKFPPRQVQWFVNNAKEEGLRASQVETY
                     DDFARKMVEFYLNYEQQCAKESVVDFAELLLRSYELLMRNEVIREHYCSRFRHILVDE
                     FQDTSKLQYKWLKLLGGTSTAVFAVGDDDQSIYAFRGAHAGNMKEFERDFRIPKIIKL
                     EQNYRSHGNILDAANALIRNNNGRLGKNLWTSEGHGEPIRVYNAPTDFDEASFIVEEV
                     KSLHREGVALAQMALLYRSNAQSRVLEHALFNASLAYRVYGGMRFFERQEIKHALAYL
                     RLIASPDDDNALLRVVNFPARGIGSRTLEQLQDEASRQGSTLWRVMRQHCKPPSSIAS
                     NGSSPAGAIDAGRGQDRARREKGVPGFVALIDGLREACEGLPLPEIVEHVLEHSGLAA
                     HYRAEREGADRLENLNELINAAASFTQEAEENGLIEFLSHASLEAGEHQAGSGQDALQ
                     LMTVHSAKGLEFHSVFLSGLEEGLFPHDNSRNETGGLEEERRLMYVALTRARRRLYLS
                     FSQSRMLHGQTHYNIPSRFLQEIPDRLLKLLQPVQKAMTASLGRFAGATPGITAPPSS
                     LNTARPAWRVGQNVIHAKFGAGVIVNCEGRGADARVEVRFGRAGTKWLLLEYAKLVPA
                     "
     gene            complement(7767..8639)
                     /locus_tag="VFI43_05975"
     CDS             complement(7767..8639)
                     /locus_tag="VFI43_05975"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET7061709.1"
                     /translation="MSQKHPVIAVTGSSGAGTSTVKNSFEHIFFREKLTAVVVEGDSF
                     HRYDRDEMKKAVAESEKDGNRPISHFGPEANEFEKLEALFKEYGETGTGKTRLYLHND
                     AEAAPYQQKAGTFTPWTSIKQGSDLLFYEGLHGGVKSETADVAKYVDLLVGVVPIVNL
                     EWIQKIHRDMNARGYSVEAVTHTILRRMHDYVHYITPQFSRTHINFQRVPTVDTSNPF
                     IARDIPTLDESFVVIRFRDPKGVNFPYLLSMIHNSFISRPNTIVVPGGKMGLAIELVL
                     TPLILDLVKASPSA"
     gene            9076..9510
                     /gene="rplM"
                     /locus_tag="VFI43_05980"
     CDS             9076..9510
                     /gene="rplM"
                     /locus_tag="VFI43_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7061710.1"
                     /translation="MKTFSAKPHEVHHEWFVIDAAGKVLGRLAAEIAYRLRGKHKPIF
                     TPHVDTGDYIVVINVDKMRITGNKAENKLYHRHSGYPGGIYQTTFAKMHARFPERPLE
                     KAVKGMLPKGPLGYAMLKKLKIYAGAAHPHAAQQPRQLEVRA"
     gene            9515..9907
                     /gene="rpsI"
                     /locus_tag="VFI43_05985"
     CDS             9515..9907
                     /gene="rpsI"
                     /locus_tag="VFI43_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7061711.1"
                     /translation="MAVNYNYGTGRRKSAVARVFIKPGKGLITVNDKPVDEFFSRETG
                     RMVVRQPLELTGNLTNFDVVVNIHGGGESGQAGAVRHGITRALIDFDASLKPLLSNAG
                     LVTRDAREVERKKVGLRKARRRKQFSKR"
     gene            10067..11095
                     /gene="argC"
                     /locus_tag="VFI43_05990"
     CDS             10067..11095
                     /gene="argC"
                     /locus_tag="VFI43_05990"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7061712.1"
                     /translation="MLKAGIVGGTGYTGVELLRMLSAHPEVSIEAITSRSEAGMAVAE
                     LFPSLRGRVTLRFSDPSAAELDKCDIVFFATPNGIAMQQARSLLNAGVRIIDLAADFR
                     IKDVAVWEKWYGVTHACPELVEEAVYGLPEVNREKIRSARLIANPGCYPTAVQLGFLP
                     LVEAGVVDLDHLIADAKSGVSGAGRKAEVHTLFSEASDNFKAYGVPGHRHLPEIRQGL
                     SAAARKPVGLTFVPHLTPMIRGIHATLYATLTSEADLQTLYETRYVDEPFVDVLPAGS
                     HPETRSVRGSNLCRISVHRPQGGNTVVVLSVTDNLVKGAAGQAIQNMNLMFGLPETLG
                     IDQAPLLP"
     gene            11266..12012
                     /locus_tag="VFI43_05995"
     CDS             11266..12012
                     /locus_tag="VFI43_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HET7061713.1"
                     /translation="MVMIRSLKRKFGISAPRVAVRPQVPGYVRWLGITVVAVLAIGLS
                     WGMYDAGRKFAGFDKNETDYELEQLSQVTARLQKENDELRMKIASLDRQSQMDHVARE
                     DISKQVKTLEGENIRLKEDLAFFQNLGSVAGKNEQGVAISGLKLERGQLPGEYRYTLL
                     LVQSGPRKKEFQGSVEFAINLQKDGKKMIMPLASESSSRVFDVRFKFYQRVERSFILP
                     PEAVVESMQIKVFENGMAQAKLTQTVNLST"
     gene            12021..12440
                     /locus_tag="VFI43_06000"
     CDS             12021..12440
                     /locus_tag="VFI43_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7061714.1"
                     /translation="MFGKKAGKPQSQIDTLIGANTRIEGNINFSGGLRVDGHIKGNVV
                     ASTDRPSILVLSDQASVEGKIQVSHAIINGVVSGSIQASEYVELQAKAKVSGDIQYKA
                     MEIHVGASVEGMLLNYGKDDPQSASKVVPLITAAHAD"
     gene            12494..12859
                     /gene="erpA"
                     /locus_tag="VFI43_06005"
     CDS             12494..12859
                     /gene="erpA"
                     /locus_tag="VFI43_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET7061715.1"
                     /translation="MNSTVETDLVEMQVPLIFTDSAANKVKELIEEEGNSDLKLRVFV
                     AGGGCSGFKYGFTFDELINEDDTVMEKNGVKLLIDAMSFQYLAGAEIDYQENAEGAQF
                     VIKNPGASSTCGCGSSFSV"
     gene            complement(12948..14045)
                     /locus_tag="VFI43_06010"
     CDS             complement(12948..14045)
                     /locus_tag="VFI43_06010"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET7061716.1"
                     /translation="MKTAYYIGMMSGTSLDGVDAALADFNRSPPSLLGTFYLPYSPDL
                     RGRLLELHQAGVDELHRAAMLGNELSRHYADAVGGLLRDSGVSRGEVRAIGCHGQTVR
                     HCPQAERAYTIQLFNPALLAELTGVTVVADFRSRDIAAGGQGAPLVPAFHSALFMHSE
                     CHRIIVNIGGICNLTSLARGQVAGYDCGPGNMLMDAWCSHHTGEPYDENGNWARSGEV
                     IESLLERFLALPFFSLPPPKSAGREQFNFTWLESCLNGNEKPEDVQATLLELTVQSIS
                     RSVFQYFPRAAELYLCGGGARNGAVITRLGLTMPGKTIELTDRLGVSADWVEAFAFAW
                     LAKQAISGVPANLPVVTGARDSRILGAIYQA"
     gene            complement(14095..15408)
                     /locus_tag="VFI43_06015"
     CDS             complement(14095..15408)
                     /locus_tag="VFI43_06015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7061717.1"
                     /translation="MDNNRGTFLRMLSAPVKKRSRWLILLSSFPLFGMMAAFGIAPDT
                     SLDSLPVQQVVLGLSLPEIHLSDDSSMTFWRQERIQRGDTIASLLSRLEVNNSDVGSF
                     LRDARSVKTMHQLVPGRIVHVQTGAAGELLWLRYFPGGSEQLTMEKSGESFTVAEQPA
                     QLETHIQMKSGVINSSLFAAVDIAGVPESVALQIVDILSSDIDFHRDLRKGDQFTVVY
                     DSLYINGEPARAGRVLAVEFVNQGTPHRALYFEADNGESGYYAPDGKNLKKAFLRSPL
                     EFSRISSGFSSARFHPILKTWRAHSGIDYVAPTGTRVKAASDGVVAFAGWQGGYGNVV
                     VLQHHGVYSTVYGHLSSFAKGLRKGQRVSQGDVIGHVGATGMATGPHLHYEFRQNGVQ
                     RDPLKVATPAANPVSSRLMPVFYDATIPLMARLDMLRGTNLAFVD"
     gene            15748..>16651
                     /locus_tag="VFI43_06020"
     CDS             15748..>16651
                     /locus_tag="VFI43_06020"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HET7061718.1"
                     /translation="MAAEDLTLDLLAFRPFWRLAAALRTRVEAIIGAWEEAVRENLPA
                     ADELTLQQLRNSLPLVLEEIAHAFASSTPKATRELIEGSKSHGTTRFHENYSIRELVV
                     EYRLLRRIVIEQLAEELGEDLDTETMVALNMALDAVLQSGVVTFTNHLQQQILAAAEI
                     QSKYLSFLSHDLRNHLSQAVMQIQLLSAKLAKAPEYAETVAGIESVKRAILRTTAGMD
                     RLLEAEQLRHGSANLDIHTVHLSQLLLEVSQQWQYEAQLKGLELRVEVPDGAKVLSDE
                     RLLTLVLQNLLGNAVKYSSAGTINI"
BASE COUNT         3833 a         4534 c         4506 g         3778 t
ORIGIN      
        1 ctccgacatg ccgtagatca acccaaaatt aatgactttt gcataacggc gctgctcgct
       61 gcttactgct tcccgcggaa ttccgaacac ttcggctgcg gtagcccgat gtatatcctc
      121 accagcgcca aatgctcgga gcaacccctc gtcatgggaa atatgcgcca tgatccgcag
      181 ctcgatctgg gaataatccg ccgacacaat ccggtatccg ggcggcgcga tgaaggcttc
      241 gcggatacgc cggccttccg ccgtgcgcac cgggatgttc tgcagattgg gttcgttgct
      301 ggcaaggcgt ccagttactg caaccgcttg cgcgtagttg gtatgaactc ttcccgttgt
      361 cgtatcgacc atgcggggaa gcttttcggt ataggtggat ctgagcttgg caaggcccct
      421 gtaatcgagc aacatctttg cgagcggata atcgagcgca agcctctgca gcacatcctc
      481 gtctgtggac ggagtgcccc ctggggtctt tttcaggacc gggagcttga gccgggtgaa
      541 cagcacctcc tggacctgct ttgtcgaatt cagattgaat ggcagcccgg ctacagcgca
      601 ggcgtgttct tccagcatca gcatcttctc cgccaattcc cggctctgta tctcgagcat
      661 tttcaaatcg agaagcactc cgttgcgctc catttcgaac agaatttcca gcaacggcat
      721 ttcaagctcg cgatagacat acgagaggct gacgtccgcg gacatctgcg ggtaaagatg
      781 ctcatgcaag catagggtaa tatccgcgtc ctcagccgcg tatctcgtgg ctcgctctat
      841 ctccacctga tcgaatccta tgcggctcgc gcccttcccc gtaacgtcat cataagtgac
      901 ggttctgatg tcgagatgcc ggagcgcgag gttgtccata tcgtgcgcgc gatgcggctc
      961 aagcacgtat gactgcaaca aggtgtcgtg aacgatgccg ttcagttcaa tgccatgatt
     1021 ggcaaaaacg tgcttgtcgt atttaaggtt ctggcccagc ttcagcttct ccctgttctc
     1081 cagccacggt ttgagcttgt ggagtacaag atcgagggga agttgcggcg gcgcgccggc
     1141 atatccatgc gccaagggta tataagcggc gcaatgacac ttcactgaaa atgcgatgcc
     1201 gaccagcctg gccttcatcg gatcgagtga ggtggtctcc gtatcaagtg ataccagggg
     1261 cgcgtcgttg agccgtgcga tccactggtc gagctccgat tcggtgagca cggcctgata
     1321 atcgaccggc ggcacatccg gcttccgagg ggggccaccg gaggaggaag tgctttcctg
     1381 atactctccc ccgctctcct ggtcccgcag ttcgcgcagc caggtcttga aatcgagccg
     1441 gtcgtacagt tccagcagtt tgctggtatt ctgtggttgc ggtcccaact cgtgcagatc
     1501 gacagggagc gggacatcgc atttgatggc aagaatttcg ggtgatttgc gtaaccaccc
     1561 gagtgccttg cgaaggtttt cacccactac accaccgatt tcatcggcgc gggcgataag
     1621 gttgtccagg gtgccatact gtgtgagcca tttgacggcg gttttgggac cgaccttctc
     1681 cacccccggt atgttgtcgg tcgcatcgcc gacaagggcc aggtaatcga gtattcgctc
     1741 gggtggcacg ccgaatcttg cccgcacgcc ggcctcatcg agaacttcgt tggtcatcgt
     1801 attgatcagc gtcacacccg gtttcacgag ctgggcaatg tccttgtccc ccgtggaaac
     1861 gatgcagcgc aaattctcag gctcagcctt ttttacgagg gttccgatca cgtcgtccgc
     1921 ctctacgcct tcgatgacca gcatgggcca ccccatggcc gcaatgcact cgtacaacgg
     1981 ttcgagttgc gccaccaagt ctcccggcat gggcggtcga tgggatttgt attcgggata
     2041 gagatcatcg cgaaaggtct tgccttttgc gtcgaaaacg caagcgctat aatctgcctg
     2101 gtaatcctta tgcaggcgac gaagcatgtt caggacgcca taaatcgctc ccgtgggctc
     2161 attattgcgg ttcctcagat ctggtaacgc gtgaaatgcg cgatacaggt aggatgagcc
     2221 atcaaccaaa agcaatgttt tcattcaact tccaagacct tcgatgaact cgcaagagaa
     2281 accagaacac ccattgcctt ctgattatcc cgaaaaagca agaaatgcca cggaatcgtg
     2341 gcgcattttt gggattatgg cagaattcgt ggaggcgacg gaacggctga attcgatcag
     2401 gccggctgtc agcattttcg gcagcgcccg tacttctccc gatcacccgt attacaagct
     2461 gacggaacag atcgctaacc agttgtcgga ggcaggcttc tccgtcattt cgggaggagg
     2521 accgggaatc atggaagccg cgaacaaggg cgcctacctc ggaccgtcgc cgagcgtagg
     2581 gctcaatatc caactccccc atgagcagca ccggaatgtc tatcaggata tcagccagac
     2641 gttccgccac ttcttcgccc ggaagtatat gttcgtgaga tttgccaccg cctacgtggt
     2701 ggttccggga ggattcggca cgcttgacga actgatggag gcactcacac tcgtccagac
     2761 cggaaaaacg cggaaaatgc ctattattct cgtctgctcg gcattctggc aagggatgct
     2821 cgactggctt cgtcagacgc tcgttggaga aggcatgatt tcgcccgaag acatggatct
     2881 catccagatt attgatgagc cgggcgaagt cgtcaaagca atcttccgat attacgaaag
     2941 ccgtggcttc gagccgtcgg cggcagagag ggaggtgcaa ctgaatctgt aatacaagcg
     3001 acatttcggc tagaatagaa tattcaccag atctgctgga ggcgccatgc gtcaaatagc
     3061 cgtcgcgcta ctgttgagtg ttgcgcttcc tgcatcggct caatccaacc aacccaagga
     3121 tttgcgccct ctgcccgaag tgcccgagcc gcccgagctc gcgttgccgg ctcctggggc
     3181 cgaccaggag gatgaggccc ttgagcccca ggttaccatc acgagacgag gggaagacca
     3241 ggtcgaggaa taccggctca acgggcggct ttacatgatc aaggtcatcc cgcgcattgg
     3301 gctgccctat tacctgatag ataattacgg ggacggcacg tttgccaccg gcaccggcgg
     3361 gcttgaccag cgcatccgtc cccccatgtg ggtcatacgg cagttctagc agctttaacc
     3421 ttgtgaaacc gtctctccag aggggatcac gatctaaacc taaaataaca gcttacctgt
     3481 gttgcttccg cctctgagtt cttccttgtc cccgcttcac agatttaaag tttcggttca
     3541 gagggtagct cggattcctg ccgcaccgcg aggaagtttt taacgaatcc ttgcttatat
     3601 cgctcctctc cggtttccat cctcatgtca gttttcacca ctgttgcccc gcatcagctt
     3661 tcactctggc tcaagaatta cccgctgggc acgttgttgg atctgcaagg catctcctcg
     3721 gggattgaaa acacgaacta ctacgtcacg accagtctgg gcaaatatat cctcacctta
     3781 ttcgaaaagc tgacggcggg cgatctaccc tattacctca atctgatggc gtatctatcg
     3841 caccaaagca tcccttgtcc aaggcccatc cccagtctca ataacgagct tcttggcacc
     3901 cttaacggca ggcccgccag catcgtcacc tgcctgcccg gaaagtctcg cgaaaaccca
     3961 tcgaccctgg attgcgcgaa agtcggaaaa ctgctcgcag acatgcatct gcgcgggctg
     4021 tcctaccccg gggaaatgca aaattcacgg gggccgagat ggtggaaagc ggccgctcac
     4081 gccgtgatgc cgtttttaac gcgcgaggag gcggatgtcc tgaaagagga gcttcatttc
     4141 cagtcgttgt atcgttttga agatctgccc cgtggcgtca ttcacgcaga tctgttccgc
     4201 gacaatgtcc tgttcgacgg tggagccgta ggcggcgtaa tcgacttcta ttttgcctgt
     4261 aatgatgtct tgttgtacga tttggcaatc acggcgaatg actggtgcct cacgagcaat
     4321 ggaaagctcg acccggatcg cacgttctcc atgcttgaag cctaccacgc ttgccgtccg
     4381 ttgacgagca ccgagcgcag ggcgtggccg gtcatgctgc gcgccgccgc gctgcgattc
     4441 tggttatctc gcctgcatga ttaccacctg ccccgggttg gggagctcac ccatgtcaag
     4501 gatcccgctc agttcatgcg tatattggcg aatcacgccg cgtcccacga cgagctggcg
     4561 aagatctggg ttgagcacac acgtaattag gagcgcgcgc aatgagcagg ggagaaagtc
     4621 caccggagat agggaacatg gatgtcaggc aagtcgatgg aaaacaggga tggcagtgga
     4681 tagtgagcgg atttctcctg ttccggaggt ttccgatggt ctggatcctg ctctgcacaa
     4741 ccctgttgct gattgccgcg acgctggccc ttattccggt ggcgggccaa ttcattttca
     4801 ccctgctttc gccggttttt ctggcgggcc tgatgattgg atgcagaacc ctggaaaaag
     4861 gcggccagct cgaaatcgca cacttgcttg ccggctttcg ctatggcgcg acgccgctga
     4921 ttaccattgg tggaatatac ctgatcggac aggttctgat atttggaatt gccatgctga
     4981 tcggcggcga aacgatgaga gatctcttga ttgcaggaag gcgcgtcgat gaaaatgagc
     5041 tgcaggaagt catgagcagc agcctgtctg cgttgctcgt ggcgcttacg ctgtcgattc
     5101 cgctgatgat ggcaacgtgg ttcgctccct tgctcgtcat attcaggaat atgcctcccc
     5161 tcgaggccat gcggctcagc tttttcgcct gcataaaaaa tatagtcgcg tttcagatat
     5221 atacattctt cctcgtagtg ctgacgatcg ttgcaactat cccgtatggc cttgggttgt
     5281 tcgtgcttgt gccgactctt tttgcttcga tttacgcgag ttacgaagat atattttcag
     5341 aaacgccagg cgtcgcgtag agctcctcgt tccactggag ccctctcatt ccacccctgt
     5401 tcaagctggg acaagcttgg catactcgag caacagccat tttgttcccg cacggccaaa
     5461 tcttacttcg acccgcgcat ccgcgccccg gccttcgcaa ttgacaatca cgccagcccc
     5521 aaatttcgcg tggatcacat tttggcctac ccgccatgcc ggccgcgccg tattaaggct
     5581 ggaaggcgga gccgttattc ccggcgtcgc tccggcaaac ctccccaggc tcgctgtcat
     5641 agccttttgg acgggctgca gtaatttgag caaacgatcg ggaatttcct gcaagaagcg
     5701 cgatggaata ttgtagtggg tttgcccgtg cagcatccgg ctttgcgaga agctcaaata
     5761 caaccgcctt ctcgcgcgtg tcaacgccac atacatcaag cgccgttcct cttcgaggcc
     5821 gccggtttcg tttctgctgt tgtcgtgcgg aaacaggccc tcctccaaac cgctcaggaa
     5881 aacgctgtgg aattcgagcc ctttggccga gtgcacggtc atcaactgga gcgcatcctg
     5941 gccacttccg gcctggtgct cgcccgcctc cagggaagca tggctgagaa attcaatgag
     6001 accgttttct tccgcttcct gagtaaagct cgccgccgca ttgatcaatt cattcaggtt
     6061 ttccagccgg tctgctccct cccgttccgc acggtagtgg gccgccagcc cactatgctc
     6121 cagcacatgt tcgactattt ccggcaacgg caacccttcg caagcctcgc gcagtccatc
     6181 gatcagtgcg acaaaccccg ggacgccttt ctctctcctt gccctgtcct gccccctgcc
     6241 cgcatcaatc gcccccgcag ggctgctccc attgctcgct attgaagagg gcggcttgca
     6301 gtgctggcgc attaccctcc aaagggtact cccctgccgg ctggcctcat cctgtagctg
     6361 ttccaaggtc cgggagccaa tgcctcgcgc cgggaagttg acaacccgaa gcagggcgtt
     6421 gtcatcatcg ggactggcta tcagccggag atacgccagc gcgtgcttga tttcctgtcg
     6481 ctcgaaaaaa cgcatcccgc cgtatacccg ataagccagc gaggcgttga acaaagcatg
     6541 ctccagcacg cgggattgcg cattggagcg ataaagcagc gccatctgcg ccaaggctac
     6601 cccctcccga tgcaatgact tcacttcttc gacgatgaag gacgcttcgt cgaaatcggt
     6661 cggggcatta tagacgcgta tcggctcgcc atgaccctcg gaggtccaca ggttcttgcc
     6721 gaggcgcccg ttgttgttgc gtatcagggc attcgctgcg tctagtatat tgccgtggga
     6781 acgatagttc tgctcgagtt tgatgatttt ggggatgcga aaatctctct cgaactcctt
     6841 catgttcccg gcgtgagcac cgcgaaatgc ataaatgctc tggtcgtcgt cgcctaccgc
     6901 gaatacggcg gtgctcgttc caccaagcaa tttgagccac ttgtattgca gcttgctggt
     6961 atcctgaaac tcgtccacga ggatgtgtcg aaaacgcgaa caataatgtt cgcgtatcac
     7021 ctcgttccgc atcagcaact catagcttcg caataacaat tcggcaaaat cgacgacgga
     7081 ttccttcgcg cattgctgtt cgtagttcag gtaaaactcg accattttgc gcgcaaaatc
     7141 atcgtaggtt tcaacctgag aggccctgag cccctcttcc ttggcattat taacgaacca
     7201 ttgcacctgc ctgggaggga atttctgatc atccaccccc aggtttttga ggatgcgctt
     7261 tatcatggcc agttggtctg cggtatcgag aatctggaaa gattgaggca gacctacatc
     7321 ctgatggtgg gcacgaagca tgcgattgca taggccgtga aaagtgccca cccacatgcc
     7381 acgagtatta accggcagca tggatgaaat tcgcatcagc atttctttcg ctgccttgtt
     7441 tgtaaaagtg acagcaagaa tgccatgggg actcacttgc cccgactgaa tcaggtgggc
     7501 aatgcgcgtt gtaagcacgc gcgtcttgcc gctgcccgct cccgcaagaa ccagcactga
     7561 ctgatggggc agcgtgactg cttcgagttg ttcgggatta agtgcggaga gtaaggaaga
     7621 catcggaaac agaccagtgg gttgggaagc gagccgttgc ccccttgaag agaatgccaa
     7681 agcggagggt gccaggctgc ctcgccatga catcccccca tttaaaattc agagtcgaaa
     7741 aagctgaaaa gccttgttca gtcagcctat gcgcttgggc tggccttgac aaggtccagg
     7801 atcaatggcg tcaataccaa ttcgatagcc agtcccatct ttcctcccgg aaccacaatg
     7861 gtattcgggc gcgatatgaa cgagttatga atcatcgaga ggagataagg aaaattcacg
     7921 cccttgggat ccctgaagcg gatcactaca aagctttcat ccagggtagg aatatcccgc
     7981 gcgatgaagg gattggatgt atccacggtt ggaacgcgct gaaaattgat atgggtgcgc
     8041 gaaaactggg gtgtaatata atggacgtaa tcgtgcatgc gccggagaat ggtgtgcgtg
     8101 acggcttcta ccgagtagcc gcgagcattc atgtccctgt ggattttctg tatccactcc
     8161 agattgacaa tcggtaccac gcctaccagc aaatcgacat acttcgccac atccgcagtt
     8221 tcactcttga ccccaccgtg caggccttca tagaacagaa gatccgaacc ctgttttatt
     8281 gatgtccatg gcgtaaacgt cccggccttt tgctggtatg gagcggcctc ggcatcattg
     8341 tggagataca ggcgcgtctt gccggttccg gtctcgccat actccttgaa caaggcttcg
     8401 agtttttcaa attcattcgc ttccggaccg aaatggctga tcgggcggtt gccgtctttt
     8461 tccgattccg ccaccgcttt cttcatttcg tcgcggtcat aacgatggaa actgtcgccc
     8521 tctaccacca ccgcagtcaa tttctcacga aaaaagatat gctcgaaact attcttgact
     8581 gtcgacgtgc cagctcccga cgaaccggtg acggcaataa cagggtgttt ttgtgacata
     8641 caggctttct cctcaggata aaaatgacta tttgtgctta tactcgccgc cggcgcttcc
     8701 gccagatggg gtcattgcat tttgcaggcc aataaaaacc tctgactatc cgctgccttc
     8761 tctcacccgc gttggaacaa cagccactca aggcgcggta caacctgcgc aaagaatgat
     8821 ggccagagga actcctggtg agatagaagc aaacgatcga tcgcgggagg attgttcaga
     8881 gcgttcagag gtttcttaac cctactataa tacctgtttt ctcggcaatt ttggacagca
     8941 gctccgcaaa gcgatgagcg ggcgaatagg agatgcgcaa acaaagcgtc aaggatcatg
     9001 gaagagcagt agatttgaca atatcaggag cgggccataa aatagacggc tttattgaaa
     9061 tagcaggtgg gttttgtgaa aacgttttca gccaaacctc atgaagtgca tcacgagtgg
     9121 ttcgtcattg atgctgctgg caaggtcctt gggcgtcttg ccgcagagat cgcctaccgt
     9181 cttcgcggca agcacaaacc catctttact ccacatgtag ataccggtga ttacatcgtc
     9241 gtgatcaatg tggacaagat gcgtattacc ggaaacaagg cggaaaacaa gctttaccat
     9301 cgccacagcg gatacccggg cggtatctac cagacaactt tcgcaaaaat gcatgcgcgc
     9361 tttccggaga ggccattgga gaaagcagtc aagggaatgc tgccaaaagg tcccctgggt
     9421 tatgcgatgc tgaaaaaact caagatttac gcaggtgctg cgcatccgca cgcagctcag
     9481 caaccacgtc aactggaagt aagggcttaa gcttatggca gtcaattaca attacggtac
     9541 ggggcgccgc aagagcgcgg tggccagggt attcatcaaa ccgggcaaag gtttgatcac
     9601 ggtcaatgac aagccggtgg atgaattctt ttcccgtgaa actgggcgca tggtggtaag
     9661 gcagccgctt gaactgacgg ggaatctgac gaattttgac gttgtggtca atattcacgg
     9721 aggcggcgaa tcaggacagg cgggcgcagt gcgtcacggc atcacacggg ccttgattga
     9781 tttcgatgcc agccttaaac ctcttttgag taatgcgggg ctcgtgacgc gggatgcccg
     9841 cgaggttgag cggaaaaaag tcggccttcg gaaggcgcgg cggcgcaagc agttttccaa
     9901 gcgatagcgt catttttcaa acaaaagccg cctgtttctg gcggcttttt tgtttgttgc
     9961 ctataatcgg tcaaccgtta tcggtcaacg tttagcgttt aaagttaaac gcgccgcctc
    10021 tgcacattaa cgtggagggt tcgttgaagg ttcgtcgagg gttctgatgc ttaaagcagg
    10081 tatagttgga ggtaccggat ataccggagt ggagttgctg cggatgttgt ccgcacaccc
    10141 ggaggtgagc atcgaggcta tcacttcgcg tagcgaagca ggcatggcgg tagcggagtt
    10201 attcccaagc ctgcggggac gagtgactct ccgattttcg gatccttctg cggcagaact
    10261 ggataaatgc gatatcgtgt tttttgcgac gcccaacggt atcgcgatgc agcaggctcg
    10321 ttccttgcta aatgccgggg tccgtatcat tgatctggct gccgatttcc gtatcaagga
    10381 tgtcgcagtg tgggaaaagt ggtacggcgt gacccacgcc tgccctgagc tggtggaaga
    10441 agcggtctat ggcctgccgg aagtcaaccg ggaaaaaata agaagtgcga ggttgattgc
    10501 caatcccggc tgctacccta ctgcggttca gctgggtttc cttcctttgg tcgaggcagg
    10561 cgtcgtcgac ctggatcacc tcatcgccga cgcaaagtcc ggtgtatcgg gggcaggacg
    10621 caaggcggaa gtgcatacgc tgttttctga agcgtcggat aattttaaag cctatggagt
    10681 acccggtcac cggcatttac ccgagatcag gcagggttta tctgcggcag ctcgtaagcc
    10741 agtgggactg acatttgtcc ctcaccttac ccccatgatc cggggcattc acgccacgct
    10801 ttatgcgacg ctgacatctg aggccgattt gcagacgctt tacgaaacgc gatacgttga
    10861 cgaaccgttc gtggacgtac tcccagcagg gtctcacccg gaaacccgtt cagtgagggg
    10921 ctcgaatctt tgccgtatct cggtccaccg gcctcaaggg ggaaatacag ttgtcgttct
    10981 ttcggtgaca gacaaccttg tcaagggggc ggcggggcaa gcgatacaaa acatgaacct
    11041 gatgtttggc ctgccggaga ctcttggaat tgatcaggcg cctttgctcc cttgaccact
    11101 aacgatcagg ttgaatgcca ggagcgcccg tgaggcgggt tgacccgata aaaatgggag
    11161 cggaccgggc ttgcgtgcaa gtctgctttt agccgaaaat acggatggag cgggattgct
    11221 ccttatcgag cggcgtcttc tccgcatcta ccggaatatg ttcagatggt catgatcagg
    11281 tcgctgaaaa gaaagttcgg tatatccgcg ccacgggtag cggtacgtcc ccaggtcccc
    11341 ggttatgttc gctggttggg aattaccgtt gttgctgtac tggcaatcgg cctttcctgg
    11401 ggaatgtatg atgcggggcg caagtttgca ggctttgaca aaaatgaaac cgactatgaa
    11461 ctggaacagc tatcccaggt aactgcccgt cttcagaagg aaaatgacga gttgcgcatg
    11521 aaaattgcca gccttgaccg ccagtcgcaa atggatcatg ttgcccgtga ggatatctcc
    11581 aagcaggtga agacgctcga aggcgagaac atccgcttga aggaggattt ggcttttttt
    11641 cagaaccttg gttcggtcgc aggaaaaaac gaacagggcg tcgccataag cggattgaag
    11701 cttgaacgcg gccagttgcc gggcgagtat cgttacacct tgctcctggt tcagagtggg
    11761 cctcgaaaaa aggaattcca gggcagcgtc gaatttgcga tcaatttgca gaaagatggc
    11821 aagaagatga tcatgccgct ggcgagcgaa agttcgtcac gggtcttcga tgttcgcttc
    11881 aagttttatc agcgggtgga aagaagcttt atcctgcccc cggaggcagt agtcgagagc
    11941 atgcagatca aggtattcga aaatggaatg gcacaggcaa agctgacaca aaccgtgaac
    12001 ctatccactt aggagatgag atgttcggaa aaaaagcagg taagcctcag agccagatcg
    12061 atacgttgat tggcgcgaat actcgcattg aaggcaatat caatttcagc ggcgggttgc
    12121 gtgtcgatgg ccacatcaag ggcaacgttg ttgcaagcac ggacaggcca agtatacttg
    12181 tattaagtga tcaggcctca gtcgaaggga agatccaggt ttctcatgca attatcaacg
    12241 gggtagtatc gggatcaata caagccagcg agtacgtgga acttcaggcg aaagcaaaag
    12301 tctcggggga tatccaatac aaagcgatgg agatacatgt gggggcatcg gtcgaaggaa
    12361 tgctgctgaa ttatggcaag gatgatccgc agtccgcgag caaggtcgtt cctctgatta
    12421 ctgccgctca tgcggattag cttcagcaaa attccctgaa taattttttc ctactttcaa
    12481 cttcaaaggc agaatgaact caactgtcga aacagacctg gtcgaaatgc aagtcccgtt
    12541 gatctttacc gatagcgcgg caaacaaggt caaggaactg attgaagaag aaggaaacag
    12601 cgatctcaag ctaagagtat tcgtagcggg aggtggttgt tccggcttca agtacggctt
    12661 tacctttgac gaactgataa atgaagacga tacggtaatg gaaaagaacg gcgtgaagct
    12721 gctgatcgat gcaatgagtt ttcaatacct ggcaggcgcg gagatcgact atcaggagaa
    12781 cgccgagggg gcgcagtttg ttatcaaaaa ccccggcgcc tccagcacct gcggttgcgg
    12841 atcttcgttt tcggtttaac ccgggcctga aatcagaaag acggaaaacg caagcgaaaa
    12901 ttccgttttg catcagaaca aaacggaggc gatcggcctg cagaacgcta tgcctgatag
    12961 attgccccaa gtattcgtga atctctagct cctgtcacga cgggaagatt cgcgggcact
    13021 ccagaaatag cctgtttcgc caaccaggca aaagcaaacg cctcgaccca atcggcgctg
    13081 accccaagcc gatccgtcag ttcaattgtc tttccgggca tggtcaatcc caaccgggtg
    13141 atcaccgcac catttcgagc tccgccgccg cagaggtata gctctgcggc ccgaggaaaa
    13201 tactggaata ccgacctcga gatggattga acagtcaact ccaggagcgt tgcctgcacg
    13261 tcctccggct tctcatttcc gttcaggcaa ctttccagcc aggtgaaatt gaactgctcc
    13321 cttcccgcac ttttcggcgg cggcagggaa aaaaagggga gtgccagaaa tctctcgaga
    13381 aggctttcga tcacttcgcc cgagcgtgcc cagttcccgt tttcatcata gggctcgccc
    13441 gtgtgatgcg aacaccacgc atccataagc atgttgccag gcccgcaatc atagcccgcg
    13501 acctgccctc gggcaagact ggtcagatta cagattccac cgatgtttac aatgatacga
    13561 tgacattcag aatgcatgaa cagcgcgctg tgaaaagccg gcaccagcgg ggcaccctgc
    13621 ccgcccgcgg caatatcacg gcttctgaag tccgccacaa ccgtgacgcc ggtaagttcg
    13681 gcaagcaagg ccggattgaa taactgaatg gtataggcgc gctccgcttg cggacagtgc
    13741 cgcacggtct gcccgtgaca tccaatcgcc ctcacttctc cccgagaaac tccgctatca
    13801 cggagcaacc ctccaacagc gtccgcataa tgccgggaaa gctcattgcc gagcatagct
    13861 gcccgatgaa gctcgtcaac tcccgcctga tgcaattcga gcagacggcc ccgcaggtca
    13921 ggactgtaag gcagatagaa agtgccgaga agagagggag gagagcggtt gaaatcagca
    13981 agtgccgcat caactccgtc gaggctggtc cccgacatca tgccaatata atacgctgtt
    14041 ttcaaagctc tcttcgaggc gctgaggcga tgcgatgaag gttagcgccg ctggctaatc
    14101 cacgaaagcg agatttgttc cgcgaagcat gtcaagcctg gccatcaggg ggatcgtagc
    14161 atcgtaaaag acaggcatga gccgcgacga aaccgggttt gccgcgggcg tggcaacttt
    14221 cagcgggtcg cgctgaactc cattttggcg aaactcgtag tgcagatgtg ggcccgttgc
    14281 cattcccgtc gctccgacat gcccgattac atctccctga ctgacgcgct gacctttgcg
    14341 caaacccttc gcaaaggatg acaggtgccc gtacacggtc gaatatactc catggtgttg
    14401 caggacgacg acgtttccat aaccgccctg ccaacctgca aaagccacaa cgccgtcgga
    14461 tgctgcctta acccgagtgc cggtcggggc gacatagtct ataccgctgt gggcccgcca
    14521 cgtcttgagg atgggatgaa agcgcgcgct ggaaaatcct gaactgatcc ttgaaaactc
    14581 gagcggcgag cggagaaagg cttttttcag attcttgcca tcgggagcgt agtagccgct
    14641 ctcaccgttg tccgcctcaa aatacaaggc gcggtgaggt gtaccctggt ttacgaattc
    14701 cacagccagc acccttcctg ccctcgcagg ctctccattg atatagagcg aatcgtagac
    14761 gaccgtgaac tggtcgcctt tgcgcaggtc ccgatgaaaa tctatgtcgg acgacaggat
    14821 atcgacaatc tgcagcgcga cgctttcagg aacgcctgcg atatcgaccg cagcaaacag
    14881 cgaactgttt atcacgcccg atttcatctg gatgtgcgtt tccagttgag cgggctgctc
    14941 agctaccgtg aagctctcac ctgatttctc catggtcaac tgctcgcttc cacccgggaa
    15001 atagcgtaac caaagcaatt ccccggcggc gcctgtctgg acgtgaacaa ttctgccagg
    15061 aacaagctgg tgcatcgttt tgacactccg ggcgtcccgc agaaaactcc ccacatcgct
    15121 gttattgacc tcgagccgcg acaacagcga tgcaatggta tctccccgct gaatgcgctc
    15181 ctggcgccag aacgtcatgc tgctgtcgtc ggatagatga atttcgggaa gactaaggcc
    15241 gagaaccact tgctgcacgg gcaggctgtc gagagaagta tccggcgcaa taccgaaagc
    15301 agccatcatg ccgaacaacg gaaagctcga taacaggatc aaccaccgtg atctcttctt
    15361 gaccggagcc gatagcatcc gtagaaaagt gcctctatta ttatccatgt ttggcaaatg
    15421 gctaatgagg gcgcaagtct aacataaaac cttatcaata cgtagggtaa agaagagagc
    15481 aatcctgttg cctgcacctg atggaactgc ttcaaccaat ctttcctcca atcaggagcg
    15541 ctgcagtcag tgttcataaa tttctctcac gcgcagacga ctctgtataa ttgcgtctgc
    15601 ctagattccc gcccctagag cttcaggttg cccaccaatg gaacagcagt gctcgtcgcc
    15661 agggcgccca cgcctacgcc aaccataacc atccaaaatc cccttgtaat tgaaaattga
    15721 ttttcagcat atacaggagc gccatacatg gctgcagaag atctcacgct tgatttgttg
    15781 gcttttcgtc ctttctggcg cttggcagcc gcccttcgaa cgcgcgtgga ggccatcatt
    15841 ggagcatggg aagaggcagt acgcgagaat ctgcccgctg ctgacgagtt gacccttcag
    15901 cagctcagga attcgcttcc tttggtactc gaagagattg cccacgcctt cgcatcgagt
    15961 accccgaaag caacccgcga actgatcgag ggcagcaaaa gccatggcac taccagattc
    16021 catgaaaatt acagcattcg tgaactcgtt gttgagtaca ggttattgcg aaggatcgtg
    16081 atcgagcagc ttgctgaaga attgggagaa gatctggata cagaaacaat ggtcgccctg
    16141 aacatggcgc tcgatgcagt tctgcagagc ggcgttgtga cttttaccaa tcatctgcag
    16201 caacagatcc tcgccgcagc cgagatacaa tcgaagtacc tttcttttct ttcgcatgat
    16261 cttcgcaacc atctcagtca ggcagtcatg caaatacagc ttctttccgc aaagcttgcg
    16321 aaggcaccag agtatgcgga aaccgtggca ggtatcgaat cggtcaaacg agctatcttg
    16381 cgaacaacgg ctggaatgga caggctgctg gaggcggagc agttgcggca tggctccgct
    16441 aatctcgata tacatactgt gcatctgtct caactgctgc tcgaggttag ccagcaatgg
    16501 cagtacgaag cacaactgaa aggattggaa ctgcgtgtgg aagtacctga tggcgcaaaa
    16561 gtcttaagcg acgaaagatt gctgacgctg gtgttgcaga atttgctggg taacgcggta
    16621 aaatacagct cagcgggtac aataaatatc t
//
LOCUS       DASSHJ010000153         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_810590, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000153 DASSHJ010000000
VERSION     DASSHJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810590"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFI43_06025"
     CDS             complement(<1..417)
                     /locus_tag="VFI43_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7061719.1"
                     /translation="MSQPMHLRHEKPLFPENARVSIAHLALPVRASHGFTRLLTVLAF
                     AITLGCSSENSWHMEEVTGHLPDLDFSLTSDRGSRVTANEFEGNLLLLYFGYTHCNAE
                     CPVSLARLARVVQMLGDGKNQARILFVSLDPGRDTPE"
     gene            726..2258
                     /locus_tag="VFI43_06030"
     CDS             726..2258
                     /locus_tag="VFI43_06030"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET7061720.1"
                     /translation="MKDHLIIFDTTLRDGEQSPGASMTRDEKIRIARQLERMRVDVIE
                     AGFPAASNGDFEAVKAVADCIRESTVCGLARAIEADIRRAGEALRGSNLARIHTFIAT
                     SPIHMEKKLRMTPDQVVEQAVKAVRWARQYTDDVEFSPEDAGRSDIDFLCRVLEAVID
                     AGAKTLNIPDTVGYTMPEQFGRLIRTLRERIPNSDKVVFSVHCHNDLGLAVANSLSAV
                     ANGARQVECTINGLGERAGNAALEEVVMAVRTRQDFFLCDTRVDTTHIVPASKLVSGI
                     TGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWGANKLLLGKHSGR
                     NAFRTRLAELGIDLRSEEALNTTFMRFKELADKKHDVFDEDLQALVSDEAMVLQEHYR
                     LLALVAHSETGETPYAQVVIAEDGRESHAQAEGSGPVDATFRAIEKILASGAELQLFS
                     VNAITSGTDSQGEVTVRLQRSGRIVNGHGADIDIVVASAKAYLSAINKLHSKLERAHP
                     QV"
     gene            2279..>3420
                     /locus_tag="VFI43_06035"
     CDS             2279..>3420
                     /locus_tag="VFI43_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HET7061721.1"
                     /translation="MESARFLDFAHYVWVRFFQHNCTQVAASLTFTTLLSLVPFIAIA
                     ISVVAAFPAFAEYSAQIKTFVLNTMVPEAANRVIAVYMQQFADNAVKLTAIGTFFLGV
                     TAVALMLTIDSALNSIWCVPRLRPLLHRVLIYWAVLTIGPLFIGVSLSLTSWLIGISM
                     GLTEDIPLVNVVLLQLVPLALTSVAFSISYLIVPNRQVQLRHAIAGGVTAAVGFEIMK
                     RVFALYISHFPTYQAVYGAFATVPIFLLWVYLSWLMVLLGAVIAASLSSWRFRRWGPN
                     PAIQGSQFLDALRILQALGEALHTGSVETYSSLQKRLDRGFEEIEPILERLVQANLVR
                     QIKAGGWVQIMDPDEVTVADIYRLFTFDPATLQPAVEDNPKLARVL"
BASE COUNT          758 a          933 c          947 g          782 t
ORIGIN      
        1 ttcgggagta tctcgaccgg gatccagcga gacgaatagg atgcgcgcct ggtttttccc
       61 atcgcccagc atttgcacga cccgggcaag gcgagccagg gaaacagggc attcggcatt
      121 gcaatgcgta tatccgaaat acaacagtaa caggttaccc tcgaactcgt ttgccgtaac
      181 ccggctgcca cggtcggaag tcagggaaaa atcaagatcc ggcagatgac ccgtaacctc
      241 ttccatgtgc catgagtttt cactgctaca tccaagcgtg atggcgaagg cgaggaccgt
      301 caaaagacgg gtaaacccat gggaagcccg aaccggcagt gccaggtgtg caatgctcac
      361 ccgggcgttt tcaggaaata agggcttttc gtgcctcagg tgcattggct gtgacatgca
      421 agatcaggcc taaaatttca ctatagcagt ccaatcatcg cagttcagag acaacgcgca
      481 gatcgccccg cttgggaatc cagaaacctc ttgcgtgaag gaaataaacc ttctatattt
      541 caagcatggc taccgctgag tattctttcc cctcccgcct cgcaaggcgt tcgcccctca
      601 aggcgaagcc cctgctgcgc ctcgcgcgct gatacatacc gcccttccag cctttcacca
      661 gcacagaatc agcttctgga ttctgtatca cagttcgagt attctttctc aagcgggaga
      721 aaacattgaa agatcattta attatttttg acaccacgtt gcgggatggt gagcaaagcc
      781 ccggcgcttc catgacgcgg gatgaaaaga tccgcattgc ccggcaattg gagcgcatgc
      841 gtgtcgatgt catcgaggcg ggcttcccgg cagcttcgaa cggcgacttt gaggcggtaa
      901 aagcagtagc cgattgcatt cgcgaaagta ccgtatgcgg cttggcgcgg gctatagagg
      961 ctgatatccg gcgcgctggc gaggccttgc ggggctcgaa tctcgcccgc atccacacgt
     1021 ttatcgccac ctctcccata catatggaga aaaagctgcg catgactccg gatcaggtgg
     1081 tcgaacaagc ggttaaagcg gtaagatggg cgcgccaata taccgatgac gttgagtttt
     1141 ctcccgagga tgcgggtcgg tcagatatcg acttcctttg ccgcgtgctg gaagctgtta
     1201 tcgatgcagg tgctaaaacg ctgaatattc ccgataccgt cgggtatacc atgcctgagc
     1261 aattcggaag gctcatccgg acgttgcggg agcgtatacc caattccgac aaggtagtat
     1321 tttccgtgca ttgccataat gaccttgggc ttgcggtcgc aaactcgctt tcagcggttg
     1381 ccaatggcgc ccggcaggtt gaatgcacga taaacggcct gggagagcgc gcgggaaacg
     1441 cagcgttgga ggaagtcgtg atggcggtcc gcacgcgtca ggattttttt ctctgcgata
     1501 cccgagtcga caccactcat atcgttcccg ccagcaaact ggtatccgga atcactggat
     1561 tccccgtgca accaaacaag gcaatcgtgg gagcaaatgc tttcgcccac gaatcgggca
     1621 ttcatcagga tggtgtgctc aagcatcggg aaacctatga gatcatgcgc gccgaggacg
     1681 taggctgggg cgcgaataaa ttactgctcg gcaagcactc ggggcgtaac gcttttcgta
     1741 cccgcctggc ggagctcggt atcgacctgc gctccgaaga agcccttaac accacgttca
     1801 tgcgcttcaa ggagttggct gacaagaagc atgatgtttt cgatgaagac ctgcaagcgc
     1861 tggtttcaga cgaggccatg gtactgcagg agcattaccg actactcgcg cttgtcgcgc
     1921 acagcgaaac gggcgaaacc ccctatgccc aggttgtcat agcggaggat gggagggaat
     1981 cgcatgctca ggctgaggga agcgggcccg tcgatgccac ttttcgtgca attgaaaaga
     2041 tcctcgcgag cggagcggaa ctgcaactat tttccgttaa tgccataacc agcggcaccg
     2101 actctcaagg agaagtcacg gtacggcttc agagatccgg ccgcatcgtc aacgggcatg
     2161 gagcggatat tgatattgta gttgcctctg cgaaggccta tctcagcgct atcaacaagc
     2221 tgcacagcaa gctggaacgg gcccacccgc aggtttaggc gccagcatgg cagaacttat
     2281 ggaatctgcg cgatttctgg atttcgccca ttacgtctgg gtgcgcttct tccagcataa
     2341 ctgcacgcag gttgctgcca gcctgacctt tacgactctc ctgtcgctgg tgccatttat
     2401 tgcgattgcc atttcagtcg tagcggcgtt tcctgccttt gctgaatatt cggcgcagat
     2461 aaagaccttc gttctcaaca ccatggtccc cgaggcagcg aatcgggtga tagctgtcta
     2521 tatgcagcag tttgcggaca atgcggtaaa gctgacagcc atcggtactt tttttctggg
     2581 cgtaacggcg gttgccctga tgctgacgat tgacagcgca ttgaactcca tctggtgcgt
     2641 gccacggtta cgccccctgt tgcaccgcgt actgatctat tgggccgtgc tcacgattgg
     2701 accattattt atcggtgtaa gcctgtcgct cacgtcgtgg ctgattggca tatccatggg
     2761 attaaccgag gacattcccc tggttaatgt cgtgcttttg caattggtac ccctcgctct
     2821 gacgagcgtt gcattttcta tttcgtacct gatcgtgcct aatcgccagg tacaactccg
     2881 ccatgccatt gcaggaggtg taaccgcggc tgtcggtttc gaaataatga aaagggtgtt
     2941 tgccctgtat atatcccatt tccccaccta tcaggcggtt tacggagcat ttgcaacagt
     3001 cccgatattc ctgctctggg tctacttatc ctggctgatg gtgttgcttg gggccgtcat
     3061 tgccgcttcg ctttcgagct ggcgctttcg ccggtggggg ccaaatcccg ctatccaagg
     3121 aagccagttt ctggacgcgc tgcgcatcct gcaagctctg ggcgaagccc tgcatacagg
     3181 aagcgtggag acttacagca gtttacagaa gcggctggat cggggtttcg aggaaatcga
     3241 gccgatactg gagcgcctgg ttcaggcaaa tctcgtccgg cagattaaag cagggggatg
     3301 ggttcaaatc atggaccccg acgaggttac agtcgcagat atctaccgcc tgttcacatt
     3361 cgaccccgca accttgcagc ccgccgttga agataacccg aaactcgcgc gagtgcttga
//
LOCUS       DASSHJ010000154         6021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1481661,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000154 DASSHJ010000000
VERSION     DASSHJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481661"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /locus_tag="VFI43_06040"
     CDS             <1..1905
                     /locus_tag="VFI43_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7061722.1"
                     /translation="NSAARSLENPLGITFSGVTTAREALQAIQEDGDIQSVVVDDTLY
                     TLKNGEKGSRNLQMTALKLVQEINRFRPELDIYILIAQEKEDEVVDALFSETVDGYFY
                     REERDYRGMYRILNAQLQEKARTPFYDQLKNYVLMAKDAWHTPGHSSGDSVRDSPWVS
                     DFYHFIGEHVFRADLSVSVPMLDSLMEPTGVIAEAQKIAAKAFGARRTFFATNGTSTA
                     NKVIFQTLLAPGEKLILDRNCHKSVHHGVVLSGAYPIYLDSSVNKKFSIYGPVPKQTL
                     FKAIEEHPDAQAMILTSCTYDGLRYDLAPIIDAAHAKGIKVIIDEAWYAFARFHPAFR
                     PTAMEAGADYATQSTHKVLSAFSQSSMIHVNDPGFNEHLFRENFNMHTSTSPQYTMIA
                     SLDVARKQAMMEGYKLLSRTLELAKELRAQINSTGVFRVLDLPDLLPAEVSGDNILLD
                     CTKVTVDISRCGFTVEDLIRELFERYNIQVEKSTFNTLTLLLTIGTTRSKVSRLYDAL
                     MRIAREGRAPRRLYRIPEIPRFTELRYLPRDAFYCGGELVPLLDEQERLNDHLDGRVC
                     ADQITPYPPGIPVLVPGQTITSEVMQYLVSMLRSQKRVEVHGIVYDGYLPCLRLLTAA
                     EEKGLKKLVK"
     gene            2144..3331
                     /locus_tag="VFI43_06045"
     CDS             2144..3331
                     /locus_tag="VFI43_06045"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HET7061723.1"
                     /translation="MCRDAFMSNLMNTYLQLPVTFARGKGVWLWDEEDKRYLDALAGV
                     AVCGLGHCHPKLVKAICNQAGTLIHTSNLYHIKRQEELARRLADLSGMDNAFFCNSGA
                     EANEAAIKLARLYGHGKKIDLPTIIVMEKSFHGRTMATLTASGNRKVQAGFEPLLTGF
                     ARVPYNDLEAVAQVGVHNKNVVAVLVEPYQGEGGVNVPEASYLQGLRHLCDQNGWLLM
                     LDEVQCGIGRTGKWFAFQHSGIFPDVITLAKGLGSGVPIGACLAKGMAAGLFKPGNHA
                     STFGGNPLACTAALTTLEVIAEEDLLRNAMELGDFIRMMLKAQLADVPDVEQVRGQGL
                     MVGIELSRPCGELVKTALEQGLLINVTSERVVRLLPPLVMQRHEAEQVVDILSRLIKK
                     FLA"
     gene            3419..4345
                     /gene="argF"
                     /locus_tag="VFI43_06050"
     CDS             3419..4345
                     /gene="argF"
                     /locus_tag="VFI43_06050"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7061724.1"
                     /translation="MQIRHFLQFNDFSREEFEHLFERTRWIKREFKQYRRYWPLEDRT
                     LAMIFDKHSTRTRLSFEAGMHQLGGAAIYLSTRDTQLGRGEPVEDAARVISRMVDIVM
                     IRTFEHEIVRRFAAHSRVPVINGLTDEYHPCQILGDIFTFIEHRGTISGKTVAWIGDS
                     NNVCNTWLQAAEVFDFNVHVSTPQGYEVEPERAGLYGTDHYEEFADPHDAVKDADLVT
                     TDVWTSMGFESETEERKKDFANFRVDTEMMACANSDTLFMHCLPAHRGEEVAAEVIDG
                     PQSVVWEEAENRLHTQKALIEYLLLGKVTSRT"
     gene            4342..5595
                     /locus_tag="VFI43_06055"
     CDS             4342..5595
                     /locus_tag="VFI43_06055"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7061725.1"
                     /translation="MRKAEKGEMSEVRKVVLAFSGGLDTSVILKWLQDAYHCEVVTFT
                     ADIGQGEEVEPARAKATQLGVKEIFIEDLREEFVRDFVFPMFRANAVYEGEYLLGTSI
                     ARPLIAKRQIEIARETKADAVSHGATGKGNDQVRFELGYYALQPDIRVIAPWREWDLT
                     SREKLLSYAEQHGIPVEMKKKNGSPYSMDANLLHISYEGRALEDPAQEPEESMWRWSL
                     SPEKAPDHAEYLELEFEQGDIVALNGEKLSPAQVLTKLNQLGGKHGIGRLDLVENRYV
                     GMKSRGCYETPGGTIMLRAHRAIESLTLDREVAHLKDELMPRYAELIYNGYWWSPERK
                     MMQAMIDASQAHVNGWVRVKLYKGNVMVVGRDSKTDSLFDPHIATFEDDEGAYNQADA
                     AGFIKLNALRMRIEANLKKRNKSVS"
     gene            5840..>6021
                     /locus_tag="VFI43_06060"
     CDS             5840..>6021
                     /locus_tag="VFI43_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HET7061726.1"
                     /translation="MPSFDIVSEVDKQEVRNAVDQVNKEVTTRFDFKGSNARVEQADY
                     VLTVFADDEFKLGQVL"
BASE COUNT         1444 a         1513 c         1736 g         1328 t
ORIGIN      
        1 aactcagccg cccgttcgct ggaaaatccg cttggcatca cattttcagg cgtcactaca
       61 gcaagagaag cgttgcaggc catccaggaa gacggcgata ttcagtcggt ggtcgtggat
      121 gataccctct acactctaaa aaatggagaa aaggggtccc gaaacctgca aatgaccgcg
      181 ttgaagctgg tgcaggaaat caatcgtttt cgccctgagc tcgatatcta catcctgatt
      241 gcccaggaaa aagaggatga ggtggtggac gccctctttt ccgaaacggt tgacggttac
      301 ttttatcggg aagaacgtga ttaccgtggc atgtatcgca tcctgaatgc ccagctacag
      361 gaaaaggcgc ggacgccatt ctacgatcag ctcaagaatt acgtgttgat ggccaaggac
      421 gcgtggcaca cgccggggca ttcttcgggc gattcggttc gcgatagccc atgggtcagc
      481 gacttttacc atttcattgg cgagcatgtc ttccgggcag atctgtccgt gtccgtaccc
      541 atgctcgact ccctcatgga gcccacggga gtgatagcgg aagcccagaa gatcgcagca
      601 aaggcgtttg gcgcgcgtcg tacttttttt gcgaccaacg ggacttccac ggccaacaag
      661 gtgatcttcc agaccctgct agccccgggc gagaagctga tactggaccg gaactgccat
      721 aaatcggtgc atcacggggt ggtgctgtcc ggcgcctacc cgatctacct cgattcttcg
      781 gtcaacaaga aattcagtat ttatggcccc gtgccgaagc agaccctttt caaggccata
      841 gaggagcatc cggacgcgca ggcaatgata ctgacgagtt gcacgtacga tgggctgcgc
      901 tacgatttag cccccatcat tgacgcggcg catgcgaaag gcatcaaggt gatcattgac
      961 gaagcctggt acgctttcgc ccgttttcac ccggccttcc gtccgacggc aatggaggcg
     1021 ggggcggatt acgctaccca gagcacacac aaggtgttat cggctttctc gcaatccagc
     1081 atgattcatg tcaatgaccc aggattcaac gagcatctct tccgcgagaa tttcaacatg
     1141 catacttcca ccagtccgca atacaccatg attgccagcc tggacgtagc tcgcaagcag
     1201 gcaatgatgg aggggtacaa gctgctttcg cggacgttgg agctggcgaa ggaattgcgc
     1261 gcccagatca attcgacggg tgtattcagg gttcttgacc ttccggacct gttgcccgcg
     1321 gaagtgagtg gagacaatat tctgctggat tgcaccaagg tgacagtgga tatatcccgt
     1381 tgcggcttta cggtggaaga tctcatccgg gaactgtttg agcgttataa catccaggtg
     1441 gaaaaatcga cgttcaatac gctcaccctg cttctcacga tcggcaccac gcgaagcaag
     1501 gtatcacgtc tttatgacgc actcatgcgt attgcgcggg agggtcgcgc tccgcgaaga
     1561 ctctaccgta taccggaaat accccgtttt accgaactca gatatcttcc gcgcgacgca
     1621 ttttattgtg gtggtgaact ggtgccgctg ctggatgagc aggaacggtt gaacgatcat
     1681 ctggatggca gggtgtgcgc ggatcagatc actccctatc ctcctggcat tccggtgctc
     1741 gttcccggtc agacgattac ttcggaagtg atgcaatatc tcgtgagcat gctgcgatcg
     1801 caaaagcgtg tcgaggtgca cggcattgta tatgacggat accttccatg cttgaggctt
     1861 ctgacggcag ccgaggaaaa aggcttgaaa aagctcgtca aataggtaaa gatacaagcg
     1921 atgctaagat tccctaagac tatcacgaca cggcggcgca tgccgccgtg ttgcattctg
     1981 cgccatgaaa tccaattcgc gatcgccgga aagcagggtt gctggcgggt cctcaaaaga
     2041 aaggaaaatc cgaaggcaaa aacctgatcc cgggtgttaa gaatttcgtg ggatttttca
     2101 aaagcagcgc acatttctcc agccaaattc agccgatgct tctatttgta gagatgcttt
     2161 tatgtcaaat ttgatgaaca cataccttca actgcccgtc acatttgctc ggggaaaggg
     2221 cgtgtggtta tgggatgaag aagacaagcg ctatcttgat gcgctggcag gcgttgcagt
     2281 atgcggtctc gggcattgcc atcccaagct tgtcaaggcg atttgcaatc aggccggcac
     2341 gctcatccat acatcaaatc tttaccatat taaacgccag gaggaactgg cgaggaggct
     2401 ggcggacctg tcggggatgg acaacgcttt tttctgcaat tccggggcgg aagcgaacga
     2461 ggccgccatc aaactggccc ggctatacgg gcatgggaag aaaatcgacc tgcctaccat
     2521 catcgtcatg gaaaaatcgt tccacggccg taccatggct accttgaccg ccagtggaaa
     2581 ccgcaaggtc caggcggggt ttgagccttt gctcacaggt tttgccaggg tgccgtacaa
     2641 cgatcttgaa gcggttgcgc aagtcggggt ccacaacaag aacgttgtcg ccgtgctcgt
     2701 cgaaccatac cagggagaag ggggcgtcaa tgtgcctgag gcgagttatt tgcagggttt
     2761 acgccacctg tgcgatcaga atggctggct gctcatgctg gacgaagtgc agtgtggaat
     2821 aggacggact ggcaagtggt ttgcctttca gcacagcggc atttttcccg atgtgatcac
     2881 actggccaaa ggtttgggat cgggggtgcc tatcggggcc tgtctcgcta aaggaatggc
     2941 agcaggactt tttaagccgg gaaaccacgc ttccacgttt ggcggaaatc cacttgcctg
     3001 cactgccgcg ctgacgacac ttgaggttat cgccgaagag gatctgctca gaaacgcgat
     3061 ggaactgggt gacttcatac gcatgatgct aaaagcgcaa ctcgccgacg ttccagatgt
     3121 cgagcaggtt cgggggcagg gcctcatggt gggtatcgaa ctttccaggc cctgcggtga
     3181 actggtaaag acggcattgg agcaaggact cctgatcaac gtgacctcgg aacgggtggt
     3241 gcgcctgctg ccgccgcttg tcatgcaacg gcacgaggcg gagcaagtgg tggatatcct
     3301 gtcccgctta ataaagaaat tcctggcttg aatgcatcac ggcggcgctt tagccgcagc
     3361 ttcaaccagt tttccgataa ccttcccaag ctgatgtacg cgcaggggag ccattttcat
     3421 gcagatcagg cattttttac agtttaacga tttctcccgg gaggaattcg agcatctgtt
     3481 tgagcgcacc cgctggatca agcgggagtt caagcagtac cggcgctatt ggccgctgga
     3541 agatcgcact ctcgcaatga ttttcgacaa gcattcgact cgcacgcggt tgtcgtttga
     3601 agctggcatg catcaactcg gtggggcggc gatttatctt tccacccgtg atacccagct
     3661 aggacgcggg gagccggtgg aagacgcggc ccgcgtgatt tcgcgcatgg ttgatattgt
     3721 catgatccgg accttcgaac atgagattgt caggcggttt gcggcccact ctcgggttcc
     3781 cgttatcaac ggattgaccg atgaatacca tccctgccag atactgggcg acatttttac
     3841 cttcatcgag catcgcggaa caatctccgg taagactgtg gcgtggatcg gggactcgaa
     3901 taacgtttgc aatacctggc ttcaggcggc ggaagtattc gatttcaatg tgcatgtttc
     3961 gaccccacag ggttacgaag tggagccgga gcgggcgggc ctttatggca ccgatcacta
     4021 cgaagaattt gccgatccgc acgacgcggt aaaggacgct gatctcgtga caaccgacgt
     4081 ctggaccagc atgggattcg aatccgaaac cgaggagcgc aaaaaagatt ttgccaattt
     4141 tcgcgtagat accgaaatga tggcgtgtgc gaacagcgat accttgttca tgcactgcct
     4201 gcccgctcac aggggagagg aagtggcggc agaggttata gatggtcctc agtccgtggt
     4261 ctgggaagag gcagagaacc ggctgcatac acaaaaggcg ctgatcgaat atttattgct
     4321 ggggaaggta acgagtcgaa catgagaaaa gcggaaaaag gggagatgag cgaagtcagg
     4381 aaagttgttc tggcgttttc gggaggattg gacacctctg tcatactcaa gtggttgcag
     4441 gacgcctacc actgcgaagt ggtcaccttc actgctgaca tcgggcaggg cgaggaagtc
     4501 gagccggcgc gcgcaaaagc aacgcagctt ggcgtgaagg aaatcttcat cgaggatctg
     4561 cgcgaggaat tcgtgcgcga ttttgtcttt ccgatgtttc gagcgaacgc ggtatacgag
     4621 ggcgaatatc tgcttggcac cagcattgcc aggcctttga tcgcaaagcg ccagatcgag
     4681 attgcgcgtg aaacaaaagc cgacgccgtt tcacatggcg caaccggaaa aggcaatgat
     4741 caagtgcgtt ttgagctcgg ttactatgca ttgcagcccg atattcgggt cattgcacct
     4801 tggcgcgaat gggacctgac ttccagggaa aagcttttga gctacgccga gcaacacggc
     4861 attcctgtgg aaatgaagaa aaaaaatggc tcgccctaca gtatggacgc caatctgctt
     4921 catatttcct atgaaggccg tgccctggaa gacccggcac aggagccgga agaatcgatg
     4981 tggcgctgga gtctttcgcc ggaaaaggcc cctgaccatg ccgagtacct ggaacttgaa
     5041 ttcgagcagg gcgatattgt tgccctgaat ggggaaaagc tttctcctgc ccaggtgctg
     5101 accaagctca accagcttgg gggcaagcat ggcataggcc gtctggatct ggttgaaaac
     5161 cgctatgtgg gaatgaaatc ccgtggctgc tacgagaccc cagggggcac tatcatgctg
     5221 cgtgcccatc gggcaatcga atccctcacg ctcgaccggg aggtcgctca tctgaaagac
     5281 gagttgatgc cccgctacgc cgaactgatt tacaacggct actggtggag tcccgagcgg
     5341 aagatgatgc aggccatgat tgacgcgtca caggcgcatg taaatggctg ggtgcgtgtg
     5401 aaactctaca aggggaatgt aatggtggtc ggccgggatt cgaaaacaga ctctctcttc
     5461 gaccctcata tcgccacctt cgaggatgac gagggcgcgt acaaccaggc agatgcagca
     5521 ggatttatca agctgaatgc actgcgcatg cgtatcgagg cgaatctgaa aaagagaaac
     5581 aaaagcgttt cgtgatagga gccattaaag gagcctgcct cagattcaga ttgttgccag
     5641 gcgaagatgc aagccgataa agatgggtga aaagcctggc aggcacagaa aaacgtgtga
     5701 tgcttttaag attgtttgga taggtattgc ctatgccaga ctgctttatg gccagtcaca
     5761 cggacgaagc ggcacatggt gttctgccag acgctaaggg aagttgctgg acaagttctt
     5821 ctatcaggag atttttccta tgccgtcatt tgatatcgtt tccgaagtcg acaaacagga
     5881 ggtccgaaac gcggtcgacc aagtgaataa ggaggtaact acccgctttg atttcaaggg
     5941 atcgaacgcg cgcgtggaac aggcggatta cgtgcttact gttttcgccg atgacgaatt
     6001 caagctgggc caggtactgg a
//
LOCUS       DASSHJ010000155         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1578658,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000155 DASSHJ010000000
VERSION     DASSHJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578658"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /gene="rfaE2"
                     /locus_tag="VFI43_06065"
     CDS             <1..1001
                     /gene="rfaE2"
                     /locus_tag="VFI43_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET7061727.1"
                     /translation="GYGIMTDKVIGTLGVLQRKKENILVIDAKSLDKYSRVRATVSKP
                     NYQELVTLLSITDPAPLGRRANQIRLYGQRLLRKTGAKYVAAPIDIEGALLFQRGKEP
                     YRTFSKPVENTKAAGAGDTYVAALTLALASGAAIEDAGEIARSAAGIILQKSGTATCT
                     QKELGHYFGSSGKYTQDWQELKRRFDFYKKEGIRTVFTNGCFDLLHSGHVTYLEQARA
                     LGDVLVLALNSDASIRRLKGSNRPINSMSERIRILSGLESVTLLTSFEEDTPAQLLDM
                     IKPDIYVKGGDYSVDKLPEAPVVLGYGGRIEIMPFVQDRSTTNIITRIKGLDKQMA"
     gene            1051..2136
                     /locus_tag="VFI43_06070"
     CDS             1051..2136
                     /locus_tag="VFI43_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7061728.1"
                     /translation="MRPDNMGDVLMSQPAMRALKEAIQGRKLTLLTSTAGASIARFIP
                     EVDEIIPFDVPWVKTGEAAQNDGKQQLLELASTLEDRRFDAAVIFTVYSQNPLPAAML
                     CYQAGIKTVLGYCRENPYQLISRWVPDREPFDYVLHEVERQLRLVESVGRATSNTRLC
                     LEFDEKSRKTVSGKIQAMLAAAGIRTDEPWFVLHAGASEEKRCYPAREYLKACRSLAG
                     QGYKIVLTGSFVEGSLVDEIAKPLGGKAVNFAGELSLPELIGLIAVAPVLVSNNTGPV
                     HIAAAVGTPVVVLYAMTNPQHTPWQVASRVLYFEVPPHLQSRNRLLQSFPGPSAPRAS
                     VEAIEMAVNELVRTDNGTKRYGTYSFL"
     gene            2099..2902
                     /locus_tag="VFI43_06075"
     CDS             2099..2902
                     /locus_tag="VFI43_06075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7061729.1"
                     /translation="MGPSVTVLIPSYNRPSTLAVTLTGLCFQRVRDFMVVISDQSSVP
                     VYQDASVQTAIRLLEQRGHQVEQLVNLPRLGLAQQRQFLLDQVESPYGLFLDDDVLLE
                     SFVIENMLQALGEEEIGFVGQALIGLSYRHDRRPGEQAIEMWESPVEPELVTPSHSAW
                     QRYMLHNAANILHIQEKLQLTPHKQKKYKIAWVGGCVMYDVQKLRESGGFEFWKEVPP
                     EHAGEDVLAQLRVMKRYGGCGLIPSGAYHQEAATTVIERKFDIPRELTI"
     gene            2948..>3759
                     /locus_tag="VFI43_06080"
     CDS             2948..>3759
                     /locus_tag="VFI43_06080"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7061730.1"
                     /translation="MNQLREAQNGKVKAPEQEDVFIQEIEELAKRAGEIVKDYYAKRG
                     PQEFKSERQVVTDADRESEKLLKEGLLRLHSCNFCGEESGGDVIGQGDQWIVDPLDGT
                     ENVAGYPPLLAISIGLLRDGKPVLGVVYDPIHHVLYSARQGGQANVNGEVTQVSKQTD
                     PARAIVAVDFSSKMETRPQTLGQLSKVLEHARAVRVLGTPALSLAAVAAGRLDLFFRP
                     STKLTDLVAGVCIVRASGGRAVDFDGTDWTIHSKGIIAGSAGMIGVYAHCFR"
BASE COUNT          912 a          932 c         1100 g          815 t
ORIGIN      
        1 atggctatgg aatcatgacg gacaaggtca tcgggacgtt aggggttttg cagagaaaaa
       61 aagagaatat tttggtgatc gacgcgaaat cactggataa atatagccgg gtcagggcta
      121 ctgtgtcgaa gccgaactac caggagcttg tcacgctgct ttccattacc gaccccgccc
      181 cgctaggccg tcgggcaaat caaatcaggt tgtacgggca gcggctcctc aggaagacgg
      241 gcgcaaagta tgtggcggcc ccgatcgata tcgagggggc gttgttgttt cagcgcggca
      301 aggaaccgta tcgaacgttc tcaaaaccag tcgagaacac caaggctgcc ggcgcgggtg
      361 atacgtatgt tgcagccctc accctcgcgc tggcaagcgg cgcggcaata gaagacgcgg
      421 gagagattgc aagatccgct gcgggaatca tcctgcaaaa atccggcacc gctacctgta
      481 cccagaaaga gctggggcat tatttcggga gcagcggcaa atacacccag gactggcaag
      541 aattgaagag gcggtttgat ttttataaaa aagaaggtat ccgcacggtc ttcacgaatg
      601 gctgctttga cctgcttcac agcgggcatg ttacctatct tgagcaagcg cgggcattgg
      661 gagatgtgct ggttttggcg ctcaactcgg atgccagcat tagacgcctg aaaggaagca
      721 accggcctat caatagcatg tctgagcgca tccgcatctt gtcaggactg gagtcggtaa
      781 cgctgctgac atcgttcgag gaagatactc ctgcgcaact ccttgacatg atcaagcccg
      841 acatatacgt caagggtggg gattattccg tcgacaagct tcccgaggcc cccgtcgttt
      901 tggggtatgg cggaaggatc gagatcatgc cttttgtcca ggatcgttcg acaacgaata
      961 tcattaccag gatcaaaggc ctggataagc agatggcgta accgggcaat ggcgtcggga
     1021 gactgggaca gcctgaggaa tatattgtgc gtgcggcccg acaatatggg cgacgtcctg
     1081 atgagccagc cggcaatgcg ggcgctcaag gaggctattc aaggcaggaa actaacgctg
     1141 ctgacttcta ctgcgggcgc atccattgcg cgttttatcc ctgaagtcga tgaaatcatc
     1201 ccgtttgatg ttccctgggt aaagacaggc gaggcggctc agaacgacgg aaagcagcaa
     1261 ctcctggagc ttgccagtac gctggaagac cggcggtttg atgctgcggt aatctttacg
     1321 gtctacagcc aaaatcccct tcccgctgcc atgctatgct accaggctgg gatcaaaacg
     1381 gttttgggct actgccgcga gaatccgtac cagttgatca gccgatgggt tcccgataga
     1441 gaacctttcg actacgttct ccatgaggtg gaacgccagc ttcgcctggt cgagagcgtg
     1501 ggccgggcga cctccaatac aaggctttgc ctcgagtttg acgagaaaag ccgaaaaact
     1561 gtatctggga agatacaggc aatgctggcg gcagcaggta ttcgcacgga tgagccatgg
     1621 ttcgtgctgc atgccggagc aagtgaagaa aagcgctgtt atcccgcgag ggagtaccta
     1681 aaggcttgcc ggtcgcttgc cggacagggc tataaaatag tgctcaccgg aagttttgtc
     1741 gagggcagtc tggtcgatga aattgccaag ccgcttggcg gtaaggcggt gaatttcgcc
     1801 ggtgagctat cgctcccgga actcattggg ctgatagcgg ttgcccccgt tctcgtctcg
     1861 aataatacgg ggcctgtaca catcgccgct gcggtaggca ctcccgtagt tgtgctgtat
     1921 gccatgacaa acccgcagca tacgccgtgg caggtcgcga gccgtgtact ttattttgaa
     1981 gtgccgccgc acctgcaatc gaggaaccgg ctgctccagt catttccagg tccctcagcc
     2041 cctcgcgctt cggtggaagc gatagaaatg gcggttaacg aactggtgag gacagataat
     2101 gggaccaagc gttacggtac ttattccttc ctataaccgt ccctcgaccc tggccgtgac
     2161 gcttaccggg ctctgttttc agcgcgttcg ggatttcatg gtcgttattt ccgaccaaag
     2221 cagtgtgccc gtttatcagg acgcttctgt tcaaactgct atccggctcc tcgagcaaag
     2281 agggcatcag gtggagcaat tggtcaacct gcctcgtctc ggcctggcgc agcagaggca
     2341 atttctgctc gatcaggtag agtcccccta tggtcttttt ctggatgatg acgttctgtt
     2401 ggagtctttt gtcatagaaa acatgctgca ggcccttggg gaggaggaga tcggatttgt
     2461 cggacaggct ctcatcggtc tttcatatcg gcatgaccgc cggcccgggg aacaggcgat
     2521 cgagatgtgg gaaagtcccg tagagcctga gctcgtcacg ccaagccaca gcgcatggca
     2581 gcggtatatg ctccataacg ctgccaatat tctccatatc caggaaaaac tccagcttac
     2641 gcctcacaaa caaaaaaaat acaagatcgc ctgggtcgga ggatgcgtca tgtatgacgt
     2701 tcagaagttg cgggaatcgg gggggtttga attctggaaa gaggtgccgc cggaacatgc
     2761 gggggaagac gtccttgctc aactgcgggt aatgaaacgc tatggcggtt gtggcctgat
     2821 cccgtccggc gcataccacc aggaggctgc caccactgtg atagagagaa aatttgatat
     2881 tcccagggaa ctgaccatat gaggaataag aggcaacgtt cacgggctgg accgtgatac
     2941 cttgagagtg aatcaactcc gggaagcgca aaacgggaag gtaaaagctc ctgaacaaga
     3001 ggacgttttt attcaggaaa tagaggaact cgcaaaacgt gcaggcgaga tcgtcaagga
     3061 ctattacgcg aaaaggggcc cccaggagtt taaatccgag cgccaggtgg ttaccgatgc
     3121 cgacagggaa tcggaaaagc tgctcaagga agggctttta aggttgcatt cctgcaattt
     3181 ttgcggggaa gaaagcgggg gggacgttat aggccaaggc gatcagtgga tagtggaccc
     3241 attggatgga acagaaaacg tagccgggta tcctccgctc cttgccattt caatcggcct
     3301 tctgcgagat ggcaagcctg tcctcggcgt tgtttatgat ccaattcacc atgttttata
     3361 cagcgcgcga caaggcgggc aagcaaatgt aaatggagag gtgacgcaag taagcaagca
     3421 aaccgatccc gcaagggcaa tcgtagcagt cgatttttcc tcaaaaatgg aaacgcgtcc
     3481 tcaaacgctc gggcaattat cgaaagtatt ggaacacgcg cgtgcagtaa gagtattggg
     3541 aactcctgcc ctatcgcttg ctgcagtggc agccgggagg ctcgatcttt ttttccgccc
     3601 atcaacaaaa ctgactgacc tggtagcagg tgtctgcatc gtaagagcct caggtggaag
     3661 agcagtagat tttgacggca cggattggac catccattca aaaggcatta ttgcgggttc
     3721 tgccggaatg atcggtgtct atgcacattg tttcaggat
//
LOCUS       DASSHJ010000156         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1311070,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000156 DASSHJ010000000
VERSION     DASSHJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311070"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="VFI43_06085"
     CDS             complement(<1..1898)
                     /locus_tag="VFI43_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7061731.1"
                     /translation="MSSVDVTKAVDYIKESTAARIEPAAVLIGPAIPNALVVARRIRS
                     VWPLGQMLFVADAPQLEKLQRELRHALMIGSNWSLIEIGDPRLAQKIQKAVYASQQRM
                     RLRTTLDRANVTLAAPKPVDSIQYRRMAISEHYLASLLEQSQDPILSLDNRNTVLYWN
                     TGAERLFQQRASSVYGTHVAELPFWSQALDSSLEKIHAGVETLTVETKCPLPDGGLDL
                     EILMSRVHDNTGSLVGTSLVLRDISSRNRMLEAERARRHEAERTSQMKDDFLAVLSHE
                     LRTPLSAVIGRTQLLKIRCRNEPDLLSSLSVIERNAKLQAKLIEDLLDSSLVVTGKLH
                     LELQLISVRQLVEATIESVRSVAEQKKITLSELFHPVAAVVHADSHRLQQVFINLLTN
                     AIKFTPQGGHIEVALSEAGSCVEVAVSDNGIGIDQEYLPFVFDKFGQEDASITRRHGG
                     LGLGLSIAKHLVKLHGGNIHASSPGRNMGARFTVSLPLHLGQVTGTEQFSPLEEPEKQ
                     YADILTGRRILVVEDVADARHLVTDVLQAYGAKVAAVESGAAALQILDSFRPHVLVSD
                     IGMPDMDGYELMERIRAQGCTSEELPALALTAFASPADREAATLAGYQTHIAKPLIVT
                     ELTAAVAR"
     gene            complement(2009..3166)
                     /locus_tag="VFI43_06090"
     CDS             complement(2009..3166)
                     /locus_tag="VFI43_06090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7061732.1"
                     /translation="MERISLKLAELKETTEQVVESVIAPLAAKVDEECVWPAHSMSAL
                     ADANLLGLQVPESLGGHGEGLLALSLLTETIGGACPSSALCFGMHCVGTAVIAAKATS
                     YQAEHYLRQIAQGKHVTSLALSESGTGANFYLSETTLTPDGDYFLVNGTKQFVTNGGH
                     ADSYVISTVCTQPTAEGDFSCLIVDRDTPGMEWLQSWQGFGMRGNSSRGLRLKDAKVR
                     QRNLLGEEGDQIWYVFEIIAPYFLMAMAGTYLGIAQSAVDAAGEHLRSRQHTHTGEAL
                     RNMGSLQTLYAKMWTSVEKTRGLLYEAAQRGDRGDPEALPFILACKADAAKTAVDVTN
                     DAMTICGGMAYRENSAVSQMLRDARAGHVMAPTTEMLNNWTGRALLGLPLL"
     gene            complement(3413..3859)
                     /locus_tag="VFI43_06095"
     CDS             complement(3413..3859)
                     /locus_tag="VFI43_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7061733.1"
                     /translation="MDDFVDSSRISGLKVLLIDNEPDALDLLESMLALYGANTIVATS
                     AIEGFNLLGVTRPDIIIADIGMPEKDGYQFMQDVRNLSPQEGGETPAIALTAFAHPED
                     RKKALRSGYQAHLSKPFEYSELLSIIENLASGSPSDDSKRMIEEDQ"
     gene            complement(4405..>4810)
                     /locus_tag="VFI43_06100"
     CDS             complement(4405..>4810)
                     /locus_tag="VFI43_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HET7061734.1"
                     /translation="FRELPAVLDALAQLETQPDLILYDGHGIAHPRRFGIASHLGVLV
                     DRPTVGVGKTRLIGRQVEPPRERGSWVALKEGEEVIGAVVRTQTGVKPIYVSTGHRVS
                     LATAVQVVLACTTRYRLPETTRQAHRLACAYQ"
BASE COUNT         1065 a         1321 c         1268 g         1156 t
ORIGIN      
        1 aaacgcgcga cagccgcggt taattcggtc acgataagtg gctttgcgat atgcgtttga
       61 tagccagcga gagtcgcggc ctccctgtca gccggactgg caaaggcggt tagggccagc
      121 gccgggagct cctcgctcgt acagccttgc gcacggattc tttccattaa ttcatatcca
      181 tccatgtccg gcatgccaat atcgctgaca agtacatgcg gacggaacga atcaagaatc
      241 tgcaacgcgg cagcgccgga ttcgactgcg gccactttgg cgccataagc ctggagaacg
      301 tcggtcacaa gatgccttgc gtcagctacg tcttcgacaa ccagaatacg gcggccggtc
      361 aatatgtcgg cgtactgctt ttctggttcc tccaatggag aaaattgctc ggttcccgta
      421 acctgtccaa ggtgcagggg cagtgagacg gtaaaccggg cacccatatt tcgtcccggg
      481 cttgaagcgt gaatattccc cccatgcagt ttgacgagat gtttggcaat cgaaaggcca
      541 agcccaagcc ctccgtggcg gcgggtaatg gaagcatcct cttgcccgaa tttgtcgaat
      601 acgaatggca agtattcctg gtcaatccct attccattgt ctgataccgc tacctcaacg
      661 caggaaccgg cctcgctcaa cgctacttca atgtggccac cttgaggagt gaacttgatg
      721 gcgtttgtaa gaagattgat aaatacctgt tgcaaccggt gcgaatctgc atgcacgaca
      781 gcggcaacag ggtgaaacag ttctgaaagc gttattttct tttgctctgc cacagacctt
      841 actgactcga tcgtggcttc caccagttgt cgtaccgata tcaattgcag ttccaggtgc
      901 agttttcctg tcacgaccag ggagctatcg agaagatctt caatcagctt tgcctgcagc
      961 ttggcgtttc tttcaatgac gcttaatgac gacaacaggt ccggttcgtt ccggcaacga
     1021 atctttagca attgagttcg cccgattacg gcactaaggg gagtgcgaag ttcatgggac
     1081 aagacagcca gaaaatcatc tttcatttgg ctcgtcctct ccgcttcatg gcgtcttgca
     1141 cgttcagctt ccagcatccg gttgcgagag gatatgtcgc gcaagacaag agaagtaccg
     1201 accagactgc cggtattatc gtgaacgcgg gacattaaaa tctccaggtc caggccaccg
     1261 tccggaagcg ggcattttgt ctcgactgtc agcgtctcga ctcccgcatg aattttctcc
     1321 agagacgaat ccagtgcctg tgaccagaac ggcagttcgg ccacgtgcgt tccataaacc
     1381 gagctggccc tttgctggaa tagccgttca gcccccgtat tccaatagag aaccgtgtta
     1441 cggttatcca gcgagagaat cgggtcttgg gattgctcca ggaggcttgc cagataatgc
     1501 tcggatatcg ccatgcggcg gtactggata ctatcaaccg gtttgggggc cgctagcgta
     1561 acattcgcgc ggtcaagcgt cgtgcgaagg cgcatgcgct gctggctggc ataaactgcc
     1621 ttttgtatct tctgtgccaa cctgggatca ccaatctcga taagcgacca gttggatcca
     1681 atcatgagcg cgtgacgcag ctctcgctgc agtttttcca actgcggagc gtccgcgaca
     1741 aaaagcattt gccctaaagg ccagacagaa cgaatgcggc gggcaacaac caatgcattg
     1801 gggatcgctg gcccgatcag cacggctgcc ggttctatcc tggcggctgt tgattccttt
     1861 atataatcga ctgccttagt tacatcaacc gacgacacgt tgagcccggc cgattgaata
     1921 tgcccttcga tgacgtcaga atagttgcta tcctgatgag catcagattc aatgactaag
     1981 acgagctgag ggttcagagg gggtaccttt aaagcaaggg gaggcccagc agggctctgc
     2041 cagtccaatt gttcagcatt tccgtggtgg gggccatgac atggccggcg cgggcgtcgc
     2101 gcagcatctg gctgaccgcg ctgttttccc tgtaggccat tcctccacaa attgtcatcg
     2161 cgtcgttcgt cacatcgact gccgtcttgg ctgcatctgc tttgcatgcc aatatgaagg
     2221 gtagcgcctc agggtctccc cggtcgcctc gttgtgccgc ttcatataag agacctcttg
     2281 tcttctcgac tgatgtccac atctttgcat atagcgtctg cagtgaaccc atattgcgca
     2341 aggcttcccc ggtatgcgtg tgctgccgcg aacgcaagtg ttcccctgct gcatctacgg
     2401 ccgactgtgc gatgccgaga taagtgccgg ccatcgccat gagaaaatac ggtgcaatga
     2461 tctcgaatac gtaccagatc tgatcgccct cttcccccag gagattcctt tgtctaactt
     2521 tagcatcttt aaggcgaagt cctcgcgatg aattccctcg cattccaaac ccctgccagg
     2581 attgcagcca ctccatgcca ggcgtgtcac ggtcaacaat gaggcaactg aaatctccct
     2641 ctgcggtagg ttgcgtacat accgtcgaaa ttacatatga atccgcatgt ccgccattcg
     2701 ttacaaactg tttggtgcca ttaacaagaa aataatctcc gtcgggcgtt aatgttgtct
     2761 ccgataagta aaaattagct cctgtaccgc tttccgaaag cgcaagagat gtgacgtgct
     2821 tcccttgggc gatctggcga agataatgct ctgcctggta gctcgttgct ttagctgcaa
     2881 taaccgcagt accaacacaa tgcatgccaa agcaaagcgc agaagatggg caggctcctc
     2941 caatggtttc agttaacagg ctcaatgcga gcaagccctc cccgtgcccg ccgagtgatt
     3001 caggaacctg aagcccgaga agattcgcat ccgccagagc gctcatggaa tgggccggcc
     3061 atacgcattc ctcgtctacc ttcgccgcaa gtggagcaat aacgctttcg actacttgct
     3121 ccgtggtttc ttttagctcg gcgagcttta aagaaattcg ttccatggtt ccttgtactt
     3181 aaaaataatt tgtaatgaca gctggacggc agtcttcatc ccgcagatgt tcgcactgtt
     3241 ttcgccgctg atactagggg atggggtatg gaacaaggca gaggcataat ggaaggaaag
     3301 tgccacagaa tcccgcgaaa ccgttacgcg ttgccaaggt tacttcggca atgcgtcttg
     3361 cagcggtttt cccggacttc tgccccatct ggtatcctta ttctcctgat cgtcattgat
     3421 cctcttcgat catccttttc gaatcgtcac ttggggagcc ggaggcgaga ttttcgatga
     3481 tgctgagcag ctcagaatat tcgaaaggct tggataaatg tgcctggtag cctgaacgaa
     3541 gggctttttt tcggtcttcc gggtgagcaa atgcggtgag cgcaattgct ggcgtctccc
     3601 ccccttcttg cggcgataga ttcctcacgt cctgcatgaa ctggtatcca tctttttccg
     3661 gcatcccgat gtcagcgatg atgatatcgg gtcgcgtaac tcccaataga ttgaagcctt
     3721 cgatcgcgct ggtggcaaca attgtgttgg ccccgtacaa agccagcatg gattcgagca
     3781 aatccagcgc gtcgggctca ttatcaatca agagcacctt tagcccggat attcgcgaac
     3841 tatccacaaa gtcgtccata ctcattccct ggaaatagat aggcttacga taaagggtat
     3901 gcttgccctt cttttgcttg aatcgccgcg gtgaagctct ataagcgtgc cgcgcgcacg
     3961 tcgaccccgt caaattgccg gtagtcgaag agtggtccaa gaacggaaaa aggcaaaccc
     4021 gcagagagca ggtcgcatat gatctgatta ggccgacaga tcggcctcag tcccgaatcc
     4081 caaggacgtg cagggcgagc gcctttagct ctgtgatagc tcggcgattc tacagcgcct
     4141 cgactatatg ggcaaggcgc aggagttgta aatttgaggg agtcttccaa agatgctcat
     4201 cccttccgtc tttccctttg aagcaacagc ggtacgacgt gtttctttgt tgatggtcaa
     4261 gcgggctgat gacaagagtt tgtagcgtgt tttcggaagt agcgcttccc tgttaattgt
     4321 tccgttcagg gtagtacgtg aaagagaaac gcgccgtggc gcattttcag cgccagcctg
     4381 acgttctctt cgaacgctcc ccagctactg atatgcgcag gcgagcctgt gtgcctgacg
     4441 ggtagtttcg ggcagccggt atctggttgt gcaggcgagt acaacctgaa ccgccgtcgc
     4501 caagctgacg cggtggccag tggacacata aatcggtttc actccggttt gcgtgcgtac
     4561 gaccgctcca atcacttctt caccctcctt cagcgcaacc cagctccctc gttccctcgg
     4621 cggttcaact tggcgtccaa taagacgcgt tttgcctacc cctacggtgg ggcggtcgac
     4681 caacactccc aggtggctgg caatgccgaa gcgccgaggg tgggcaatcc catggccatc
     4741 gtaaaggatg agatcgggct gcgtctccag ttgcgccagc gcgtccagca cggcaggcag
     4801 ctcgcggaag
//
LOCUS       DASSHJ010000157         5094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1462088,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000157 DASSHJ010000000
VERSION     DASSHJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462088"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3435
                     /gene="dnaE"
                     /locus_tag="VFI43_06105"
     CDS             <1..3435
                     /gene="dnaE"
                     /locus_tag="VFI43_06105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7061735.1"
                     /translation="EGMPALALTDLSNLFGLVKFYQTARSAGIKPVVGCDAWISNEAD
                     RDKPSRILLLCQSYSGYLLLCRLISRAYRENQYRGRAEIKKSWFGEDGTAGLLALSGA
                     SVGDVGMMLTQNDMAQAEALAKEWAGLFPGRFYIELQRAGHPNAESLVQRSLALASTL
                     RLPVVATHPVQFLNPEDYRAHEARVCIAEGYVLGDRRRPRHCTEEQYFKSQAEMTQLF
                     ADIPAALANTVSLAKRCNLTLELGINRLPAFPTPNNESLEDYLRSQAAAGLETRMKAL
                     FPDAAQREAQMQKYGARLEFEADVIIQMGFAGYFLIVADFINWAKNNGVPVGPGRGSG
                     AGSLVAYSLGITDLDPLRYDLLFERFLNPERVSMPDFDIDFCQDGREHVIEYVKQKYG
                     AESVSQIATFGTMAAKAVVRDVGRVLDLPYSFVDQLAKLVPFEIGMTLKKARQDEPQL
                     NERAQAEEEVRNLLELAERLEGITRNVGMHAGGVLIAPGKITDFCPIYSPGGDSVVSQ
                     LDKDDVEKVGLVKFDFLGLRTLTILDWTVRYIRQRKQTTEPANDPSQPSRTFSLETLP
                     LEDQGAYALLREGNAVGIFQFESRGMKDLLQKARPDCFEDIIALVALYRPGPMSLIPE
                     FVERKHGKRVEYLDPRLRPILEPTYGVMVYQEQVMQIAQVIGGYSLGKADLLRRAMGK
                     KKPEEMALHRDIFISGAMSNGLTEKDAAELFDLMEKFAGYGFNKSHAAAYALIAFQTA
                     YLKAHYPAEFMAATLSADMDDTDKVHGLFEDSLANKLVMLPPDINLSDYRFVPVDSKT
                     IRYGLGAIKGTGESAIQAIIEARKNGPFTDLFDLCHRVDKRIVNRRVVESLIRSGAFD
                     AIDTHRARLLATVGIALESAEQAGRAANQVSLFGESDTSIKQTSRINVSEWSEKEKLA
                     NEKAALGFYLSGHPFNAYAGDVRQFARTSLNKLSPQRETQLLAGIVYAIRIQMTRRGR
                     MSVVVLDDGSARVELVVYSELFESVRAWLKEDQLVVAEAKVFNRGGDGEYGGELRISA
                     DQLYSFEEARSRFAKRMEIHCNGLANSARLTELLTPYRGGANGIRSNGIPSNGIQSRE
                     SRNGASNSTCAVRVVYRNGSASCELDLSDEWRVSLRESLLQSLKANFSEENVRVIY"
     gene            3610..4365
                     /locus_tag="VFI43_06110"
     CDS             3610..4365
                     /locus_tag="VFI43_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7061736.1"
                     /translation="MEPNTTSIASGLERVKARIEAIAKKTGRSPESITLLAASKTNPA
                     ECLREAFVAGQRIFGENYLQEALTKMPQLTDLPIEWHFIGPIQSNKTRRIAENFAWVH
                     SIDRKKIADRLSKDRPESLPPLQVCVQVNVSGEDSKSGVAPEEATDLAAHIAGLPRLK
                     LRGVMAIPELTKATALQRNQFRMLKDVYDRLKQAGHDVDTVSMGMSEDLDVAIEEGAT
                     LVRVGTAIFGPRRYAIPEELAAEAAKAKASEKA"
     gene            complement(4362..4616)
                     /locus_tag="VFI43_06115"
     CDS             complement(4362..4616)
                     /locus_tag="VFI43_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HET7061737.1"
                     /translation="MALMITDECINCDVCESECPNSAISQGEEIFVIDPALCTECVGH
                     HETSQCVEVCPVDCIILNPDVAETREQLYEKYLSITGQPA"
     gene            complement(4624..>5094)
                     /gene="coaD"
                     /locus_tag="VFI43_06120"
     CDS             complement(4624..>5094)
                     /gene="coaD"
                     /locus_tag="VFI43_06120"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7061738.1"
                     /translation="YPGTFDPITRGHEDLVRRAARLFDQVVLGVAVSSSKKPFFTHEE
                     RVEMAREVLADCPNIQVTAFSGLLMEFAQRQNARVILRGLRAVSDFEYEFQMAGMNRS
                     MYPGVETVFMTPSEQYMFISASIVREISLLGGSVEKFVSPLVAERLRTRSRNNA"
BASE COUNT         1157 a         1401 c         1491 g         1045 t
ORIGIN      
        1 gaaggcatgc cggccttggc cctcactgat ctttcaaatc tcttcggcct ggtcaagttt
       61 taccagacgg cgcgtagcgc ggggataaaa cccgttgtcg gctgcgacgc ctggatcagc
      121 aacgaagccg atcgcgataa accgtcgcga attctcctgc tttgccagtc ctactccggc
      181 tatctgctgt tgtgccggct tatctcgcgc gcctatcggg aaaaccagta cagggggcgg
      241 gccgagataa aaaaatcctg gtttggcgag gatggaacgg ccggactgct cgcactatca
      301 ggtgctagcg tcggcgatgt cggcatgatg ctgacgcaga acgatatggc acaggcggaa
      361 gctctggcaa aagaatgggc aggcctgttc cccggccgtt tctacattga attgcagcgg
      421 gcggggcatc ccaatgctga gagcctcgtg cagcgttcac tggcgctggc ttccaccctg
      481 cgcttgccgg tggtcgcgac acatcccgta cagttcctga acccggaaga ctaccgggct
      541 catgaggcac gggtttgcat tgcggaaggc tatgtgctcg gcgaccgccg gcggccccgg
      601 cactgcacgg aagagcagta tttcaaaagc caggctgaaa tgacgcaact gttcgcggat
      661 attcccgcgg ccctggcaaa cactgtgtcg ctggcgaagc gatgcaatct cacgctggaa
      721 cttggtatca accgcctccc cgcgttcccg acgcccaaca acgaaagtct tgaggattat
      781 ttgcgcagtc aggctgcggc tggcttggaa acgcgcatga aggcgctttt ccccgatgcg
      841 gcgcagcgcg aggcgcaaat gcagaagtat ggcgccaggc tggaattcga ggcagacgtt
      901 atcatacaga tggggttcgc cggttatttt ctgattgtcg cggatttcat caattgggca
      961 aagaacaacg gcgtgcctgt gggaccaggc cgtggctctg gagcaggctc gctggtcgct
     1021 tattcgctgg gcatcacaga cttggacccc ctgcgctacg acctgctgtt cgagcgcttt
     1081 ctcaatccgg aacgcgtgtc gatgcccgac ttcgatatcg atttctgcca ggacgggcgt
     1141 gagcacgtca tcgaatacgt gaagcagaag tatggggctg aaagcgtttc ccagattgcc
     1201 accttcggta ccatggccgc caaggcagta gtgcgcgacg tgggacgtgt gctcgatcta
     1261 ccctatagct tcgtcgatca actcgccaag ctggtgccgt tcgagatcgg catgaccctc
     1321 aaaaaagccc gccaggacga gccccaactc aatgagcgtg cccaagccga agaagaggtg
     1381 cgcaatctgc tggaactggc ggagcgcctg gaaggaataa cgcgcaacgt gggcatgcac
     1441 gcgggcggcg tgctgattgc ccctggcaag ataacagatt tctgcccaat ctattccccc
     1501 ggcggggact cggttgtcag ccagctcgac aaggacgacg tcgagaaagt cggcttggtg
     1561 aagttcgact ttctggggct gcgcaccctc accatactcg actggacggt acggtatatc
     1621 cggcagcgga aacagactac ggagccggca aatgacccat cacagccgtc cagaacattc
     1681 tctctcgaga ccctgccgct cgaggatcag ggcgcgtatg ccctcctgcg cgagggcaac
     1741 gctgtcggga tattccagtt tgaatcgcgc ggcatgaagg acctcctgca aaaggccagg
     1801 ccggattgtt tcgaggacat catagcgctg gtggccttgt atcgtcctgg gccgatgtcg
     1861 ctcattcctg agtttgtcga acgcaagcat ggcaagcgcg tggagtatct cgatccacgg
     1921 ctgcggccca tccttgaacc cacctacggg gtgatggttt accaggagca ggtgatgcaa
     1981 attgcccagg tgataggcgg gtacagcctg ggcaaagcgg atctgctgcg tcgtgccatg
     2041 ggcaagaaaa agcccgaaga aatggccctg catcgcgata tctttatttc gggtgcgatg
     2101 agcaatggct tgacggaaaa agacgcggca gagctgttcg acctgatgga aaagtttgcg
     2161 ggttatggtt tcaacaaatc gcatgcggct gcctacgccc tcatcgcttt ccagactgcc
     2221 tacctgaagg cgcattatcc agctgagttc atggcggcca ccctctctgc cgacatggac
     2281 gacaccgaca aggtgcacgg cctgtttgag gacagtcttg ccaacaagct tgtcatgctt
     2341 ccccccgata tcaacttgtc cgactaccgc tttgtgccgg ttgacagtaa aacgatacga
     2401 tatggcctcg gggcgatcaa aggcacgggt gagtcagcga tccaggcgat tatcgaggcg
     2461 cggaagaacg gacccttcac cgatctgttc gatctatgcc accgtgtgga caaacgtatt
     2521 gtcaaccgcc gcgtggtgga atcgctgata cgttcaggcg cctttgatgc tatcgatacg
     2581 catcgggccc gccttctggc caccgtaggt atcgcgcttg aatcagcgga gcaggcgggt
     2641 cgcgcagcca accaggtaag tttgttcggc gagtcagata cgtcgataaa gcagacaagc
     2701 cggattaatg tctcggaatg gtctgaaaag gaaaaactgg caaacgaaaa ggctgcgctg
     2761 ggcttctact tgagcgggca tccgttcaat gcctatgcgg gggatgtcag gcagtttgcg
     2821 cgcaccagcc tgaacaagtt gagtccacaa cgtgaaacgc agctcctggc cggcattgtc
     2881 tatgcaatcc ggattcaaat gacgcggcgc ggcaggatga gcgtggtggt attggacgat
     2941 ggcagcgcgc gtgttgaact ggttgtgtac agcgaattgt tcgaatccgt ccgggcgtgg
     3001 ctaaaggaag accagttggt ggtagccgaa gccaaggttt tcaacagggg gggcgacggc
     3061 gaatatgggg gagaactgcg tatttcggcc gaccagttgt atagcttcga ggaagcgcgc
     3121 tcacgcttcg ccaagcgaat ggaaattcat tgcaacggac tggcgaattc agcgagatta
     3181 acggagctgc tcacccccta ccgaggcggg gccaatggta tccgctcgaa tggtatcccg
     3241 tcgaacggca tccaatccag ggagagcagg aacggggcga gcaattcaac ctgtgcggtg
     3301 cgcgtcgttt atcgaaacgg gagcgcgtca tgcgagcttg atctgagcga cgaatggcgc
     3361 gtcagtcttc gcgagagcct gctgcaatcg ctcaaggcaa acttcagcga ggaaaatgtt
     3421 cgagtcatct actgatgctg ctcttgtccc tgatgctgcc cttgtcccgc aaaaggggtg
     3481 gctctgcctg cgtgaacgtg atatgacgcg ctttttccac tgttttcttg accatgaaga
     3541 aacggcatcc tactatgttc gttttatttt tattggaggt tgccggctca ccggttaatt
     3601 aaaagggaaa tggaacccaa cacaacaagc attgcctccg gcctggaaag ggtcaaagct
     3661 cgtatcgagg cgattgcaaa aaaaacgggc cggtcacctg aatcgatcac gcttcttgct
     3721 gcaagcaaaa ccaatcccgc agagtgcctg cgggaagcat tcgtagccgg gcagaggata
     3781 tttggtgaaa actacctgca ggaagcgctg acgaaaatgc cgcagcttac cgacctgccc
     3841 attgaatggc actttatcgg ccccatacaa agcaacaaga caaggcgaat cgcagaaaac
     3901 ttcgcctggg tgcatagcat cgaccgtaaa aaaatcgccg atcgcttgtc gaaagaccgg
     3961 cctgaatcct tacccccgct tcaggtttgc gtacaggtca acgtgagcgg tgaggatagc
     4021 aaaagcggtg tcgcccctga ggaggcgact gacctggctg cgcatattgc gggactgcca
     4081 cgcctgaagc tacgcggagt aatggccatc ccggagctga ccaaagccac ggctttacag
     4141 cgtaaccagt ttcgcatgct caaagacgtt tatgaccggt tgaagcaggc cggccacgat
     4201 gtcgacacgg tttcgatggg catgtccgag gacctcgatg ttgccattga agagggggca
     4261 acgctggtgc gggtaggcac tgccattttc ggaccgcgac gctacgccat tccagaagag
     4321 ctggccgccg aggcagccaa ggcaaaggcc tccgaaaagg cctaggcagg ctgcccagta
     4381 atcgaaagat acttctcgta aagctgttcc cgggtttcag caacatccgg gttaaggatg
     4441 atgcaatcca ccgggcagac ctccacgcac tgggatgttt cgtggtgccc cacgcactcc
     4501 gtgcaaagcg ctgggtctat cacgaagatt tcctcgccct gcgaaatagc cgaattcgga
     4561 cattcggatt cgcacacgtc acaattgata cactcgtctg taatcattaa agccatagaa
     4621 acctcaagcg ttattccttg atctggtacg gagccgctcg gcgaccaggg gacttacaaa
     4681 tttttcgaca ctgcctccca gcagtgaaat ttcacgaaca atcgatgcgg aaatgaacat
     4741 gtattgctcg gagggggtca tgaagaccgt ttcgactccg ggatacatgc tccggttcat
     4801 ccccgccatc tgaaactcgt actcgaaatc ggacactgcg cgcaagccgc gcaaaatgac
     4861 tcttgcattc tgccgctgcg caaattccat cagaagaccg gagaaagcgg tcacttggat
     4921 attggggcaa tcggccaaca cttcccgcgc catttccacc cgctcctcat gggtgaagaa
     4981 gggtttcttg ctgctgctga ccgctacccc caggacgact tggtcaaaca ggcgggccgc
     5041 acgccgaaca aggtcttcgt ggccgcgagt gatggggtcg aaggtgccag gata
//
LOCUS       DASSHJ010000158         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_106, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000158 DASSHJ010000000
VERSION     DASSHJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFI43_06125"
     CDS             complement(<1..727)
                     /locus_tag="VFI43_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7061739.1"
                     /translation="MSANTVAFAYLIASVFFILALKGLSSPVSARRGNLFGMVGMAIA
                     VATTLTITKNWALILACIAIGGVIGAIVARRVQMTAMPELVAFMHSLVGLAAVFIAIA
                     AVNNPVSFGLPAVLPMGSKLELFLGTFIGAMTWSGSVIAFLKLSGRMSGAPITFGGQH
                     MVNLILAIVMVTFGLWFFFSETTNWTAFAAMTLIAFVLGFLIIIPIGGADMPVVISML
                     NSYSGWAAAGIGFSLGNPMLIIAG"
     gene            complement(727..1035)
                     /locus_tag="VFI43_06130"
     CDS             complement(727..1035)
                     /locus_tag="VFI43_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7061740.1"
                     /translation="MIGEVDPTIINLTVFVLAVFVGYHVVWNVTPALHTPLMSVTNAI
                     SGIILVGAMLAAGPAEADWGVWLGAVAVTLAAINVFGGFLVTQRMLEMFRKKDKKGSQ
                     "
     gene            complement(1129..2259)
                     /locus_tag="VFI43_06135"
     CDS             complement(1129..2259)
                     /locus_tag="VFI43_06135"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET7061741.1"
                     /translation="MQIGIPAEIRGGETRVAATPETVKKFTAKGLNTVLVQSGAGAAA
                     SIPDEAFQAAGATVAANAAELYDQSQIVLKVRGPDPGELELMRKDAVLVGLLAPQNPE
                     GIEMLARHGLTAFAMERLPRISRAQSMDVLSSQANIAGYKAVLMAANVYQKFFPMLMT
                     AAGTVKAARVLILGAGVAGLQAIATAKRLGAVIEAFDVRPAAKEQVESLGAKFVEVPL
                     SEEEKAQAETAGGYAREMSEDYKRRQGELVHQRAAAADVIITTALIPGRPAPVLIKEE
                     TVREMKPGSVIVDMAVEAGGNCPLSELDKIVVKHGVHLVGIANLPGLVAADSSALYAR
                     NLMNFLNLMLDPKTGEFKINREDEIIAGTLVCTGGEVIGKKV"
     gene            complement(2525..>3803)
                     /locus_tag="VFI43_06140"
     CDS             complement(2525..>3803)
                     /locus_tag="VFI43_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HET7061742.1"
                     /translation="EDDKNFKLHGKYFSACDENGDPVSPPRGNPKPGTASVLMSRNED
                     GRFISFFGDLHPSYSGNVVKAMSSAKQGYPVVNGVLQRMEPASTKPGWLFFAEINGRL
                     RATVHKVERLTPNIIEVVVHSPMAVERFHPGQFYRFQNFASLATTAGDTKLAMEGIAL
                     TGASVDVPRGLVSLIALEMGGSADLCAKLKPGDPVVLMGPTGTPTEIPSNETVVLVGG
                     GLGNAVLFSIGAAARAAGSKVLYFAGYKKLIDRYKVAEIEAAADVVVWCCDEAPGFQP
                     SRAGDLSFAGNIVDAMVAYGSGALGPQKVRLSEADRIVAIGSDRMMAAVGAARHNKLQ
                     PYLKTDHFAIGSINSPMQCMMKEICAQCLQPHKDPATGEITYVFSCFNQDQPLDQVDF
                     GGLASRLRQNSVQEKLTMRWISHCLKESSGIKV"
BASE COUNT          842 a         1145 c         1012 g          804 t
ORIGIN      
        1 atccggcaat aatcagcatg ggatttccca gcgagaaacc gattcctgcc gccgcccagc
       61 ccgaatatga gttcagcatc gaaatgacca ccggcatatc ggccccgccg atgggaatga
      121 tgatgagaaa accgagcacg aaagcgatca gggtcattgc ggcaaaagca gtccaattgg
      181 tggtctcgct aaagaagaac cacaagccga aagtgaccat gactatcgcc aggatcaggt
      241 tgaccatgtg ctggccgcca aaagtaatcg gcgcgccgct catgcggccg gacaacttca
      301 agaatgcgat gacggagccg gaccaggtca tcgcaccgat aaaggtaccc agaaacagct
      361 ccagcttgct tcccatcggc agcacggcag gcaatccgaa tgaaacgggg ttgtttactg
      421 ctgcaatagc aatgaacacg gccgccaggc caaccagcga gtgcatgaag gcgaccagtt
      481 cgggcattgc cgtcatctgc acgcgccggg cgacgatcgc gccgatgaca cccccgatgg
      541 cgatacaagc gagtatcagc gcccaattct tggtgatcgt aagggtcgtg gcaacggcaa
      601 tcgccatgcc gaccataccg aaaagattgc cccggcgcgc gcttactggg gagctcaggc
      661 ctttcagcgc gagaataaaa aataccgacg ctatcaggta tgcgaatgca accgtatttg
      721 cagacattac tggcttccct tcttatcttt tttcctgaac atttccagca tgcgctgcgt
      781 gacaaggaat ccaccgaaaa catttatggc cgccagtgtt acggccacag ccccgagcca
      841 cacgccccag tccgcttcag caggtcccgc tgccagcatt gcaccgacca gtatgattcc
      901 ggatattgca ttcgtaaccg acatgagtgg cgtgtgcagg gccggcgtaa cgttccacac
      961 gacgtgataa ccgacaaaaa ccgccagaac aaaaaccgta agattgataa tggttggatc
     1021 gacttcgcca atcattggct gctccttctt atcgtgactt gtaaacgggg aaccagtaaa
     1081 tcggtacgaa acaaggggtc ggccttgact ctacatctgc cacaagtttc agactttttt
     1141 tcctatgact tccccacctg tgcagacaag cgtccccgca atgatttcgt cttcacggtt
     1201 gatcttgaac tcgccggtct tcggatccag catcaggtta aggaaattca tgaggtttcg
     1261 tgcatagagg gcgcttgaat cggccgcaac caggcctgga agatttgcaa taccgacaag
     1321 gtgcacgccg tgcttgacca ctatcttgtc cagttcggac agggggcagt ttcctccagc
     1381 ctccaccgcc atatcgacga tgacggaacc tggtttcatc tcgcggactg tctcctcctt
     1441 gatcaaaacc ggggcggggc ggccgggtat gagcgctgtc gtgatgatga cgtcggccgc
     1501 ggctgcccgc tgatgcacca gttcgccctg gcggcgcttg tagtcctctg acatttcacg
     1561 ggcataccct ccggctgtct cagcctgcgc cttctcttcc tcgctcagcg gaacctccac
     1621 gaatttggcg ccaaggcttt ccacctgctc cttggccgcg gggcgcacgt cgaaggcctc
     1681 gatcactgca cccagccgct ttgccgtcgc aattgcctgc aggcccgcta ccccggctcc
     1741 gagaatcagc acccgcgccg cctttaccgt ccccgccgcg gtcatgagca tagggaagaa
     1801 tttctgatag acattggcgg ccatcagtac ggccttatag cccgcgatgt tggcttggga
     1861 cgacaggacg tccatgcttt gggcacggga aatgcgcggt agccgttcca tcgcaaaggc
     1921 cgttaaacca tggcgcgcga gcatttcgat accttccggg ttctgaggcg caagcaagcc
     1981 cacgagcacg gcatcctttc gcatcaattc cagctcgcca ggatcggggc cgcgaacctt
     2041 cagcactatc tgcgactggt catataattc cgctgcattt gccgccacgg tcgcccctgc
     2101 cgcctggaat gcttcatcgg gaatactagc cgcggcgcct gcgcccgact gtacaagaac
     2161 tgtgttaagt cctttcgcgg taaatttttt taccgtctca ggcgttgccg caacacgcgt
     2221 ttctcccccg cgaatttccg caggaatgcc tatctgcatt gataatctcc ctttttatcc
     2281 ttccgccgct tattcttcat gttcatgtgt gcatagcgtg acgccagccg attggcaaaa
     2341 agctgggatt ataaatgaat gccgaattcc cggctgtccg gattgacgaa gctcatcccc
     2401 agcggtagcg gcaggacgat tgcggtggaa cgcgggatga tcactagcag gcgatcatgt
     2461 ccgggttcct tcagccgccc cgcctcctca acacctagac tctcagcatc cagaccctca
     2521 gcgcctagac ttttatgccg ctggattcct tcaagcaatg gctgatccaa cgcatggtca
     2581 atttttcctg aacgctattc tggcgcaatc gcgatgccaa tccgccgaaa tcgacctggt
     2641 cgagaggctg gtcctggttg aaacaagaga acacgtaagt gatttctccc gttgccggat
     2701 ccttgtgagg ctggagacat tgggcgcata tctccttcat catgcattgc atgggcgaat
     2761 tgatcgaacc gattgcaaag tgatccgtct ttaaataagg ctggagcttg ttgtggcgag
     2821 ccgcgcccac tgccgccatc atgcggtccg agccgatggc aacaatgcga tcggcctcag
     2881 agagccggac tttctgtggt ccgagcgcgc cactgccata cgcaaccatg gcatcgacga
     2941 tattgccggc aaaactcagg tcgccggccc gggaaggctg gaaaccaggc gcttcatcgc
     3001 agcaccatac caccacgtcg gcggccgcct cgatctcggc gaccttgtag cggtcgatga
     3061 gctttttgta tccggcaaaa tacaagactt tcgatccagc cgcacgtgct gccgcaccaa
     3121 tggaaaacaa tacggcattc cccagcccgc cacccaccag aacaacggtt tcgttcgacg
     3181 gaatctccgt gggggtgccg gtcgggccca tcagtacgac tggatcgccc ggcttgagct
     3241 tcgcgcataa atctgctgac ccacccattt ccagggcaat cagcgagacg agtccgcgtg
     3301 gcacatctac tgaagcgcct gtcagcgcga tgccctccat ggccagcttg gtatcgcccg
     3361 cagtagtggc gagcgatgcg aagttctgga agcggtagaa ctgtccggga tggaagcgtt
     3421 ccacggccat gggcgaatgt acgacaactt caataatgtt gggtgtaaga cgctcgacct
     3481 tgtgaacagt ggcacggagc cgtccgttga tctcagcaaa aaacagccag cccggctttg
     3541 tggaagccgg ttccatacgc tgcaaaacgc cgttcacgac cgggtagcct tgcttggcgc
     3601 tggacatcgc cttgacgaca ttacccgaat aagatggatg caagtcgccg aagaagctga
     3661 tgaaacgccc atcttcgtta cggctcatca acacgctggc tgtcccgggc ttcgggttgc
     3721 cgcgcggtgg actgacggga tcgccatttt cgtcacaggc ggagaaatac tttccatgca
     3781 gcttaaaatt cttgtcatcc tcg
//
LOCUS       DASSHJ010000159         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_947932, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000159 DASSHJ010000000
VERSION     DASSHJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947932"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="fliD"
                     /locus_tag="VFI43_06145"
     CDS             <1..657
                     /gene="fliD"
                     /locus_tag="VFI43_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HET7061743.1"
                     /translation="DGVAADITLSAGTYASADALAAEVQTKMNGAPNLAAVNVSVRVS
                     SSGGTLTIGSASYGSSSSVNIIGGNGVASLIGASPVAALGVDVAGTINGVAAIGSGQV
                     LAATPGGAAEGLRVAIKGGTPGARGNVNFSRGYADQLSKFAADLLASDGVISSRMDGM
                     NASIKDIDRRQENLTQRLGEIEARYRTQFTALDTMLSGLNQTSQFLQQQLATLPKNNS
                     "
     gene            700..1188
                     /gene="fliS"
                     /locus_tag="VFI43_06150"
     CDS             700..1188
                     /gene="fliS"
                     /locus_tag="VFI43_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HET7061744.1"
                     /translation="MYSKSGNAVNAYANVGLETGVLAASPHKLILMLFQGAHVALSSA
                     LVHMKNGDVNAKGAAISKAIAIITSGLQASLDVETGGELAQQLNGLYDYMGRRLLQAN
                     LHNDPGHIEEVSRLLGELSGAWESIGLSGLPQGMRAHTGDRVAISTGQAAPVSSSPEA
                     FV"
     gene            1185..1523
                     /locus_tag="VFI43_06155"
     CDS             1185..1523
                     /locus_tag="VFI43_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HET7061745.1"
                     /translation="MMTGIEIISAFQSISSFTGDMAEAARAGEWDRLSDLEGRCALVV
                     AQLKAAQPFLPLTGEMQRQKVALINKILADDAAIRRYTEPWMERLHALLSSTSKSRRV
                     HIEYCQDPIR"
     gene            1569..2684
                     /locus_tag="VFI43_06160"
     CDS             1569..2684
                     /locus_tag="VFI43_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HET7061746.1"
                     /translation="MEAVNPLAPIAAVRPAATESDNRNLNAGSRFLAQLAPGQRARAT
                     VREILSNSDFIVTLESEGGKALKDQLMHITLPPGVRPDDRLSLVLISREPRPQFALLA
                     DLRTHGDVFWPSETGGFIDRLLRSPSLANRFIHSSIAPLLGGPPDDPAELSLRLGQTL
                     ERSGLFYESHMVQWIAGKRALADLLLEPQARFPRRGSLAVPGGDQNAKVGGTSVRAAP
                     STQDSGRPNSDFVNDASVRPDQAPREALALMRQQLEVLETRQVAWQGEAWPGQPVALE
                     FFEEDRGGSPDTDQPGQAPVTGWKTCLRLNLPSLGKITADLRLNTQGLEVSLAATDLA
                     AAAVLQSGTIGLGRSLESAGITLLALGVDVDEEIPSA"
     gene            2662..2973
                     /locus_tag="VFI43_06165"
     CDS             2662..2973
                     /locus_tag="VFI43_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET7061747.1"
                     /translation="MKKSHPREVAVALAYHAGQPAPQVVARGQGLIAEAIIREARNHG
                     IYVHESPELVSLLMQVDLDQHIPPQLYRAVAELLAWLYRMENRTENTIAPPAEPSNDP
                     V"
     gene            3185..>4038
                     /locus_tag="VFI43_06170"
     CDS             3185..>4038
                     /locus_tag="VFI43_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET7061748.1"
                     /translation="MCRIISSIFASTTVLAMLTCGSAVAADPECSPFLTPPVFTGTVP
                     SPKEVLGFPIGAREVTTQESDKYLDAVDASSARVVTGTAATSVEGRVLRYAIIGREGH
                     VTEQGLADIRKAVQQLTDPDTKASEAAQLAAATPAILWVSGNVHGNEKSGADAALRVT
                     YELSDRNDCVVNRILDNAIVVVLPIQNPDGREANTRRNFYGFDMNRDWFARTQPETDG
                     KLELLRQYRPVLYIDAHEFGYSHYFFPPNADPVYHEVPDRPFDWINNIYGASIAAEMD
                     RQKISYFHG"
BASE COUNT          876 a         1110 c         1166 g          886 t
ORIGIN      
        1 gatggcgtgg cagccgacat aaccctttct gctggaacct atgcctcagc agatgcgctg
       61 gcagcagagg tgcaaaccaa gatgaacggc gcgcccaacc ttgctgcggt aaatgtgtcc
      121 gtccgcgttt catcttccgg aggcacgctc actatcggct cagcaagtta cggatcctcc
      181 tccagtgtca acatcattgg gggcaatggc gtcgccagcc tgatcggtgc aagtcccgtc
      241 gcagcccttg gagtggatgt cgcgggcact atcaacggcg tagctgccat cgggtcagga
      301 caagtcctgg ctgctacccc gggaggagcg gcagaaggtt tgcgcgtagc gatcaagggc
      361 ggcaccccgg gcgcacgtgg caatgtgaat ttttcacggg gttacgcgga ccagctcagc
      421 aagtttgccg ccgatttact ggccagcgat ggggtcatct caagccggat ggacggcatg
      481 aatgccagca tcaaggacat cgatcgccgg caggagaatc ttactcaacg cctcggtgag
      541 atcgaagcgc gctaccggac ccagttcacg gccctggata cgatgctgag tgggctgaat
      601 cagacgagcc agtttctgca gcaacagttg gcaaccttgc cgaagaataa ttcctagcgt
      661 aagcaattat caaacccgga tccatccagg agaaaatcga tgtattcaaa atcaggcaac
      721 gcagtcaatg cttacgccaa tgttggcctt gaaacaggcg tccttgccgc cagccctcac
      781 aaactcatcc tgatgctttt ccagggagct cacgtggcgc tatcttcggc tctggtccat
      841 atgaaaaacg gcgatgtaaa tgccaaggga gcagccattt cgaaggctat tgcaatcata
      901 acgtccggtc tgcaggccag cctggatgtg gaaacgggag gggaactggc gcaacagttg
      961 aacgggctgt acgattacat ggggcgccgg ttgttgcagg ccaacctgca taacgatccg
     1021 ggccacatcg aagaagtttc ccgcttgctg ggggaattga gcggggcttg ggaatcgatc
     1081 ggtttatccg gtttgccgca gggcatgagg gcccacaccg gggatcgtgt agcgatttcg
     1141 acagggcaag ccgcaccggt cagtagttca cccgaggcat tcgtatgatg acgggcatcg
     1201 agatcatttc ggctttccag tctatttcca gttttacggg tgatatggcc gaagctgcgc
     1261 gagcaggaga gtgggatcgg ttatcggatc tggaaggacg atgcgcgctt gtagtggcgc
     1321 aactcaaagc cgcgcagccg tttttgccgc ttaccggcga gatgcagcgg cagaaagttg
     1381 cgctgataaa caaaatcctt gcagacgacg cagcgatacg gcgctacaca gagccctgga
     1441 tggagcgcct ccatgctctg ctcagcagta cttcgaaatc ccgtagagtt catatcgaat
     1501 actgccagga cccgattcgg taagcttcat ggattacaag ctcgtccggt caccccaaag
     1561 ttgctgaaat ggaggcggtc aatcctcttg ctccgatagc tgcagttcgc cctgcggcaa
     1621 ccgaaagtga caatcggaac ctgaacgcag gctcgcggtt tttggcgcag cttgcgcctg
     1681 gacagcgcgc gcgggcaaca gtgcgtgaga ttctctccaa ttccgatttt attgttaccc
     1741 tcgagtcgga aggtggaaag gcattgaagg atcaactcat gcacataacc ctgcctcctg
     1801 gagtgcgacc cgatgatcgt ttaagtctgg ttttgatcag ccgcgaacct cgtccgcaat
     1861 ttgcactgtt ggcggatctg cgcactcacg gggacgtttt ctggccaagc gagaccggtg
     1921 gttttattga tcgccttctt cgcagtccct ctctcgcaaa ccggtttatt cattcgtcga
     1981 ttgctcccct gcttggcggg ccgcctgatg atcccgccga gctctccttg cgtcttggcc
     2041 aaactttgga aagaagcggg cttttctatg aatcacacat ggtgcaatgg atagcaggta
     2101 aacgagctct tgcggactta ctgctggagc cgcaggcacg ctttccacgc cgtggctcgc
     2161 tcgcggttcc tggaggcgac caaaatgcga aggtaggggg tacttcggtg cgcgccgcgc
     2221 cttccaccca agatagtgga cgtccgaatt ctgatttcgt caatgatgct tctgttcgtc
     2281 cagatcaggc tccccgcgag gcgcttgcac tgatgcggca gcagctggag gtcctggaaa
     2341 cacgccaagt ggcttggcaa ggcgaagctt ggccgggaca gccggtcgcg ctggaatttt
     2401 tcgaggaaga ccggggtggg tcccccgata cagatcaacc tggtcaagct cccgttacag
     2461 gatggaagac gtgcttacgc ttgaatctgc caagcttggg caaaattaca gccgatctgc
     2521 gactaaatac gcaaggactg gaagtaagtc tggcagcaac tgatcttgcg gccgctgcag
     2581 tgctgcaatc tggcacaatc ggacttgggc gcagccttga gtcagccggg attactctgt
     2641 tggccttggg agtggatgtg gatgaagaaa tcccatccgc gtgaagtcgc ggtggccctg
     2701 gcttaccatg ccggtcaacc cgcgccacaa gtggtcgcaa gggggcaggg cctcatcgca
     2761 gaggcgatta ttcgggaagc caggaatcac ggcatctatg tgcacgaatc acctgagctg
     2821 gtttccctgt tgatgcaggt tgacctggat cagcacatcc cgccccagct ctatcgcgca
     2881 gtggcggagc tgctggcatg gctctacagg atggaaaaca gaacggaaaa tacgattgct
     2941 ccgccagcag aacccagtaa cgatccggta taggaatatt tcttcttgtt cctgcgcgca
     3001 tttactcgtt ggatcggtgc tcatccgctt tgccccgctt ttgaagaacg ccaagtgatt
     3061 ttatccggca tcacgcaatc ctgatccttg ccgctgcaca ggagcaacac taaactaaac
     3121 gcggtttgat ttcatggtct aggcgagcca tactgaattc tcagttggac gaagcgggtc
     3181 tcatatgtgc cgaattatat cgagtatctt cgcctccacc acggtgctcg ccatgcttac
     3241 ctgtggcagt gccgtggcag ccgatcccga atgctcgcct ttcctgacac ctccagtttt
     3301 taccggcacc gttccttctc ccaaggaagt gctgggcttt ccgattggcg cacgtgaagt
     3361 cactacgcaa gagtcggata agtatctgga tgctgtcgat gcaagcagcg ctcgcgttgt
     3421 gaccgggact gccgcaacct cggtggaagg gcgggtgttg cgatatgcga ttatcggccg
     3481 ggaggggcac gtaacggagc aagggcttgc cgatatccgc aaagcggtac agcaattgac
     3541 tgatcccgat accaaggctt cggaggccgc gcaacttgct gcggctacgc ctgcaatcct
     3601 ttgggtgagc ggcaatgtgc atggcaacga gaagagcggt gctgatgctg cgctgcgcgt
     3661 cacgtatgaa ctgtcggacc ggaacgattg tgtcgtaaac cgtattctcg ataacgccat
     3721 cgtcgtggta ttacccatcc agaatccgga cggccgggaa gcgaacacgc gccgcaattt
     3781 ctacggcttc gacatgaacc gggactggtt tgcgcgcacg caaccggaaa ccgacgggaa
     3841 gcttgagctt ttgaggcagt atcgtccggt gctttatatt gatgcccatg aattcggcta
     3901 cagccactat ttctttccac ctaacgccga tcctgtctat cacgaagtgc ctgaccgtcc
     3961 tttcgactgg atcaacaata tctacggggc gtcgatcgcc gcggaaatgg accgccagaa
     4021 aatttcctac ttccacgg
//
LOCUS       DASSHJ010000160         6155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1350270,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000160 DASSHJ010000000
VERSION     DASSHJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350270"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFI43_06175"
     CDS             <1..402
                     /locus_tag="VFI43_06175"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HET7061749.1"
                     /translation="EPGDEREVELVEYGGRRRVVGFNGLVNGGLTARWTVKEALERAR
                     SLEFKGMENLDREEAGVIERQAIEDQGKHPSKEHGEHPFKDHGKHAGGQEQQPVQGTH
                     QHSSGSEGQHSGGSQGHGTQSKNEGKKGGKP"
     gene            399..2117
                     /gene="ureC"
                     /locus_tag="VFI43_06180"
     CDS             399..2117
                     /gene="ureC"
                     /locus_tag="VFI43_06180"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET7061750.1"
                     /translation="MTTVDREKYADLYGPTTGDRFRLADTELICEVERSLLIPGEEVI
                     YGGGKTVRDGMGQVPGLRNDEGVLDLVITSVIVMDPILGIVKADIGVKNGRIAGIGKS
                     GNPNIMDGVDRRLVVGAGTETISGEGLIATPGAIDPHVHFISPQQAVHALSNGITTLI
                     GGGTGPADGSKGVTATPGPWNIHRMLEACEDMPVNIGLLGKGNSSLPETLREQIEAGA
                     SGLKVHEDYGSTTAAIDCALRVADEYDIQIAIHTDSLNECGYVGDTISAIDGRAIHTY
                     HTEGAGGGHAPDIMAVAGLPNVLPSSTNPTKPYTTDTVNNLFYMTMVTHHLNPGNPED
                     VAFAHSRIRAETMAAEDVLQDMGAISMFSSDAQAMGRIGETVTRCFQTADKMKRQTGK
                     LKEDSADNDNFRVLRYLAKLTINPAITHGMAEHLGSLEVGKIADIVLWPTNMFGVKPK
                     LIVKGGLINWSIMGDPNASIPTPEPVFYRPMFGANGRAIKTTCFTFMSGASIELGVPE
                     KLCLEKRILPVRNTRTLQKSHMVRNDALPHIEIDPETYQVYVDGTLATVPPADRLSLA
                     QYYFIV"
     gene            2117..2866
                     /locus_tag="VFI43_06185"
     CDS             2117..2866
                     /locus_tag="VFI43_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET7061751.1"
                     /translation="MHEVVALAAGMENDSTEGVDRAGSSAFLTSLQLADSFFPSGLYT
                     LSYGLEAFAQSGLLRANGLEALLMDYLRHAVGPSDGAALACAHRGIETGDLDVAAEAD
                     IRLAGVKLPKEARTSSQRLGRQLLKTAVIVFGGELIASYLDHVNRSELPGNHSVALGL
                     IAAAHGVEREQAVMGELYAFASGALAASVRLAVIDHLFAQAVLHRLKPVISEVGLRQC
                     ESRVQDIAGSAPLIDIMAMQHERASLRLFVS"
     gene            2900..3610
                     /gene="ureG"
                     /locus_tag="VFI43_06190"
     CDS             2900..3610
                     /gene="ureG"
                     /locus_tag="VFI43_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET7061752.1"
                     /translation="MNYQLHEHRHSHGHHTHSHDGHEFGLPSGKTRDVPCIGIGGPVG
                     SGKTALIEALVPILMERGHRPVVITNDIFTQEDANHVRRTLSGILDPARVVGVETGSC
                     PHAAVRDDPTMNLAAVEEIMQAFPDADIILLESGGDNLTLSFSPVLADYFIFVIDVGE
                     GDKIPRKRGPGVTHSDLLVINKIDLAPYVRASLEVMKRDARLVREERPFLLTNCLTGE
                     GLQAVADMIEGAKTRSPA"
     gene            3607..4554
                     /locus_tag="VFI43_06195"
     CDS             3607..4554
                     /locus_tag="VFI43_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET7061753.1"
                     /translation="MKKILFQERASQDWYTPSGLPAEVLAWGTPVRNLGVGASGKVGL
                     LDLDFSVSGGATRLVNQFQQLPLYVFHPLYIDPKRRDMAFIYLLQSGEGIVQGDRYRL
                     DLDCGPETAVHFTTQAVTKIYGMQDNFATQIVNLNAGPGTFVEYLPDPTMPFRGSRFY
                     QRMIFNVDPSATVIVGETLLPGRVARGEIHEYCFYYTDLEARSPDGALLFADRLKLGD
                     GLGHAASPGLLGSFAILAGFYVITRQLPPSTLATRLHDALAAETDVLAGISELPNKCG
                     VFARIMGHTSAIIEAAMKRVWGEARRVLFNLPVPDLRKM"
     gene            complement(4590..5639)
                     /gene="nagZ"
                     /locus_tag="VFI43_06200"
     CDS             complement(4590..5639)
                     /gene="nagZ"
                     /locus_tag="VFI43_06200"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET7061754.1"
                     /translation="MPIGPIMLDIRGTQLTAAERQKLKHPLVGGVILFTRNYSSFEQL
                     AELTAEIHALRTPPLLIAVDHEGGRVQRFREGFTRLPPMRELGIIWNDHPATARHLAK
                     AAGYVLAAELRAAGVDFSFTPVLDMDYGQSTVIGNRAFHDKPLVIAELAQQLMNGLKL
                     GGMAAVGKHFPGHGYIRADSHTDLPVDERPYREIEANDLVPFRKLIDSGLPAIMPAHV
                     IYPQVDSRPAGFSGVWLKEILRGNLGFEGCIFSDDLSMQGASIAGGPFQRAHAALEAG
                     CDMVLVCNDPDAAETLLEELEWDISALGTARLARMHGRPHPDSIVKLRENGDFVSAVE
                     KIAGLGFHNAVLPFG"
     gene            complement(5672..6049)
                     /gene="acpS"
                     /locus_tag="VFI43_06205"
     CDS             complement(5672..6049)
                     /gene="acpS"
                     /locus_tag="VFI43_06205"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7061755.1"
                     /translation="MIYGIGTDLVEPERIARLLEKYGDRFARRLLTKDEWAEYASSPR
                     PALFLAKRFAAKEALSKAFGTGIRHPVSLSHIGISHDDLGKPYFSFHPDLTAFAQNEG
                     ITGHHLSISDELNLVCAFVVLEK"
     gene            complement(6046..>6155)
                     /locus_tag="VFI43_06210"
     CDS             complement(6046..>6155)
                     /locus_tag="VFI43_06210"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET7061756.1"
                     /translation="ISELNIGHAIVAHAVFVGFAQAVREMKALMLEARK"
BASE COUNT         1343 a         1664 c         1834 g         1314 t
ORIGIN      
        1 gagcccggtg acgagagaga agtggaactt gtcgaatacg gcggcaggag gcgcgtggtc
       61 gggttcaatg gccttgtcaa cgggggtctg actgcgcgct ggacggtcaa ggaggcattg
      121 gagcgtgcgc gctcgctgga attcaagggt atggagaacc tggatcgtga agaggcgggc
      181 gttatcgaac gtcaagccat cgaagaccag ggcaagcacc cgtccaagga acacggcgaa
      241 cacccattca aggatcatgg caagcacgcg ggaggtcaag aacagcaacc ggtccagggc
      301 acgcaccagc actccagcgg gagcgaaggc cagcactcgg gtgggagcca gggacatgga
      361 acccaaagca agaatgaagg caagaagggg ggcaagccat gaccactgtt gatcgtgaaa
      421 aatatgctga tttatatgga ccgaccacgg gtgaccggtt tcgtcttgcc gatacagagc
      481 tgatatgcga agtcgagcgt tctttactga tccctgggga ggaagtcatc tacgggggcg
      541 ggaagacggt gcgcgatgga atgggccagg taccgggcct caggaatgac gaaggggtgc
      601 tcgacctggt catcacctcg gtgatcgtca tggatcccat tctcggtatc gtaaaggcgg
      661 atataggcgt caagaacggg cggatcgctg gcattggcaa atccggtaat cccaacatca
      721 tggacggtgt tgaccggcgc cttgtggttg gagcaggcac cgagacgatt tcgggcgagg
      781 ggctgatcgc gacgcctggg gctatcgatc cgcacgttca tttcatttcc cctcaacagg
      841 cggtgcatgc gctgagtaac ggcatcacga ctttgattgg cggcggtaca gggccggccg
      901 acggctccaa aggagtgacc gcgacgcctg gcccatggaa tatccaccgc atgctcgaag
      961 catgcgagga tatgccggta aacattggct tgcttggcaa aggaaacagc agcctcccgg
     1021 aaacgctccg ggaacagatc gaggctggcg ccagcgggct aaaagttcac gaagactatg
     1081 gatcaacgac tgctgcaatc gattgtgcgc ttcgcgtcgc ggatgagtac gatatacaga
     1141 ttgccataca caccgactcc ctcaatgaat gcggttacgt cggagatacg attagcgcca
     1201 tcgatgggcg ggccatccac acctaccata cggagggcgc agggggcggg cacgcacccg
     1261 acatcatggc ggtcgccgga ctgcccaatg tgctgccatc ctcgaccaac cccaccaagc
     1321 cgtacacgac cgacacggtc aacaatctgt tctatatgac aatggtgacg catcacctga
     1381 atccgggaaa tcctgaggac gttgcttttg cacacagccg catccgcgcc gaaacaatgg
     1441 cagcagagga tgtgttgcag gatatgggcg caatcagcat gttttcctcc gatgcccaag
     1501 caatgggtcg catcggagaa acggtcaccc gttgctttca gactgccgac aagatgaagc
     1561 gccagacggg taaattgaag gaggacagtg cggataacga taatttccgc gtgcttcgtt
     1621 atcttgccaa gctcacgatc aatcccgcca ttacgcatgg gatggcggag cacctggggt
     1681 cccttgaagt cggcaagatc gccgacatcg tgctctggcc caccaatatg tttggagtga
     1741 agcccaagct catagtgaag gggggattga tcaactggtc gatcatgggt gatcccaatg
     1801 cctcgattcc tacccccgaa ccggtttttt accgccccat gtttggcgcc aacggaaggg
     1861 caatcaagac aacttgcttt acgttcatgt ccggcgcttc gatagagctc ggcgtgccgg
     1921 aaaaactgtg cctggagaag cggattctcc ccgtccgtaa cacgaggacg ctgcaaaaaa
     1981 gccacatggt gcgcaacgac gccctcccgc atattgaaat cgatccggaa acataccagg
     2041 tctacgtcga cgggacactt gcaaccgttc cacccgcgga ccggctttcg cttgctcagt
     2101 actatttcat tgtgtgatgc acgaggtggt ggcgctggca gcggggatgg agaacgattc
     2161 aacggagggt gtcgatcgtg cggggtcgag cgcgtttctg accagccttc aactcgccga
     2221 ttcttttttt ccaagcgggc tttataccct ttcttacggc ctggaggcat tcgctcaatc
     2281 ggggctgtta agggcgaatg ggcttgaggc gttgctgatg gattacctgc ggcacgccgt
     2341 agggccctct gatggtgctg cgcttgcctg cgcgcatcgt gggatcgaaa cgggggatct
     2401 cgatgttgcg gcggaagcgg acatcaggct agccggggtg aagctgccga aagaggcgcg
     2461 cacaagttcc cagcggctcg gccggcagtt attgaagaca gccgtcatcg tttttggcgg
     2521 agagttgatc gcttcttacc tggatcacgt taatcgttcc gagctgccgg gcaaccactc
     2581 cgtagccttg gggttgattg cggcggcaca tggagtagag cgtgaacagg cggtcatggg
     2641 cgagttgtac gcctttgctt cgggcgcgct tgcggcgtca gttcgcctcg ccgtcataga
     2701 tcatttgttt gcccaggccg tgctgcatcg gttgaagcct gtcatctccg aggtcggtct
     2761 ccgccagtgc gagagccggg tgcaggacat tgccggtagc gctccattga tcgacatcat
     2821 ggcgatgcag cacgaacggg ccagcttgcg gctgttcgtg agctagaacg cgacaattgc
     2881 tagaacaggg agaatcgaaa tgaattatca gcttcatgag cacaggcact ctcacggcca
     2941 tcacacccac agtcatgacg gacacgaatt cgggctcccg tcagggaaaa cccgggatgt
     3001 tccttgcatc ggcataggcg gccccgtcgg ttcgggaaaa acggccctta tcgaggcctt
     3061 ggtacccata ttaatggagc ggggtcatcg cccggtcgtc atcaccaatg atatcttcac
     3121 gcaggaggat gcaaatcacg tgcggcgcac gctatccggc attctcgatc ctgcgcgggt
     3181 ggtgggcgtt gaaacgggtt cctgccctca cgctgcggtg cgtgatgatc cgacaatgaa
     3241 ccttgctgct gtcgaggaaa tcatgcaggc ctttcccgat gctgacatca ttttgctgga
     3301 aagtggcggc gacaatctga ctttaagctt cagcccggtg cttgcggact actttatctt
     3361 cgtgatcgac gtgggcgagg gcgacaaaat tcctcgaaag cggggccctg gggtaacgca
     3421 ttctgacctt cttgtgatca acaagatcga tctggctcct tacgtgcgcg cgagcctgga
     3481 agtcatgaag cgcgatgcgc gtctcgtgcg tgaagagcgc ccgtttctcc tgacgaactg
     3541 tctcactggg gaagggctgc aggcagtggc cgacatgatt gagggcgcaa agacacggtc
     3601 acccgcttga aaaaaattct attccaggaa cgagccagcc aggattggta caccccttcc
     3661 ggtctacctg ccgaggtgct cgcgtgggga acgccggtgc gaaaccttgg cgttggcgct
     3721 tccggaaaag tcggattgct tgatctcgat ttttctgtat cgggtggcgc gacgcgtctt
     3781 gttaaccagt ttcagcagct gccgctttac gtcttccatc ccctttatat cgacccaaag
     3841 cggcgcgaca tggcgttcat ctacctgctt cagtcgggag aggggattgt gcagggggat
     3901 cgatatcgac tggatctgga ttgcgggcct gagacggcgg tgcatttcac cacccaggca
     3961 gtcaccaaga tatacgggat gcaggataat ttcgctacac agatcgtcaa cctgaatgcc
     4021 ggtccaggca catttgtcga ataccttccc gaccccacca tgccgtttcg aggctcacgg
     4081 ttctatcaac ggatgatttt taacgttgat cccagcgcga ctgtcatcgt tggcgagact
     4141 ttgctgcccg gacgtgttgc ccgaggcgag atacatgaat attgcttcta ctatacagat
     4201 ctcgaagctc gctcgcctga tggcgccttg ctttttgcag accgcttaaa gcttggggac
     4261 ggcctgggtc acgcggcgtc acctggttta ctcgggtctt ttgcgatatt ggccggtttt
     4321 tacgtgatta cccgccagtt gcctccctct acccttgcaa cgcgactgca tgatgcgctc
     4381 gctgcagaga cggatgtgct cgctggcatc agcgaacttc caaataagtg cggtgttttc
     4441 gcgcgcatta tgggacatac gtctgcaata atagaggccg ccatgaagag ggtctggggg
     4501 gaagcaaggc gcgtactgtt taacctgcca gtacctgatc ttcgcaagat gtagcttgcg
     4561 aagatcgtcg cttgcagacg ccggaggtgc tacccaaacg gcaagacggc gttatgaaac
     4621 ccgaggcccg cgattttctc gacggcgctc acgaaatcac cgttttcccg cagcttcaca
     4681 attgaatcag gatgaggccg gccatgcatc cgggcgagcc gggctgttcc gagggctgaa
     4741 atatcccact cgagttcttc cagcaacgtc tctgccgcat caggatcgtt gcatacgaga
     4801 accatatcgc atcccgcttc aagcgccgcg tgcgcccgct gaaatgggcc gccggcgatc
     4861 gatgcccctt gcatgctcaa atcgtcgctg aagatgcagc cctcaaagcc caagttcccg
     4921 cgcaggattt ctttaagcca gaccccagaa aagccggccg ggcgggaatc cacttgagga
     4981 taaatgacat gagcgggcat gattgccgga agaccggaat cgatcagctt gcgaaatgga
     5041 acgaggtcgt tcgcctcgat ctctcgatag ggccgctcgt ccacaggtag gtcagtatgc
     5101 gagtctgcgc gaatatagcc gtgccctgga aaatgtttcc caacagctgc cattcccccc
     5161 agcttcaatc cattcatgag ttgttgggcc agttcggcga taacgagcgg cttgtcgtgg
     5221 aaggcgcgat ttccgatgac agtgctttgt ccgtaatcca tgtcaagcac gggcgtgaaa
     5281 ctgaaatcga ctcctgcggc tctcagttca gcagccagga catatccggc ggctttcgcc
     5341 agatgccggg cggttgcagg atggtcattc cagataatgc ccagttcgcg catgggcggc
     5401 aggcgggtga agccctctcg aaagcgctgg acgcgtccgc cctcatgatc gacggcgatc
     5461 aggagcggag gtgttcgcaa tgcgtggatt tctgcagtga gttctgcgag ttgctcgaag
     5521 gaggaatagt tcctcgtaaa gaggatcacc ccgccgacga gcgggtgctt gagcttttgt
     5581 ctctcggcag cagtgagctg ggtgccgcgg atatccagca tgatgggacc aatgggcatc
     5641 agataggcgt caggttggat atagatatgg attacttttc aagaacgaca aaagcgcata
     5701 ccaggttaag ctcatcactt atcgataaat gatgtccggt tatgccttca ttttgcgcga
     5761 atgcggtcag atcagggtga aacgagaaat agggtttgcc cagatcgtcg tggctgatac
     5821 cgatatggct caagctcaca gggtggcgga tacctgtccc aaatgctttc gacagggctt
     5881 ccttggcagc aaaacgcttg gcaagaaaca gggcagggcg gggactgctc gcgtattcag
     5941 cccattcatc tttggtaagc aagcgcctgg cgaagcggtc cccatacttt tccagcaagc
     6001 gcgctatgcg ctcgggttcg accagatcgg taccgatccc gtaaatcatt tgcgggcttc
     6061 gagcatcagt gctttcattt ctcgaactgc ctgcgcaaag ccgacaaata ctgcatgtgc
     6121 cacgattgca tgtccgatat tcagttctga aattc
//
LOCUS       DASSHJ010000161         9017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1541168,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000161 DASSHJ010000000
VERSION     DASSHJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541168"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFI43_06215"
     CDS             <1..251
                     /locus_tag="VFI43_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET7061757.1"
                     /translation="DTSVLKFLDSLENHKTVVLDAPPTAENLALAAFHILHSAYGDIY
                     GNHLRLERVRLFETPNCWADAVREHLPSARPYPVVSGV"
     gene            374..850
                     /locus_tag="VFI43_06220"
     CDS             374..850
                     /locus_tag="VFI43_06220"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7061758.1"
                     /translation="MVNQPVPDFELPSTAKKNFQLSAATGKTLVIYFYPKDDTPGCTT
                     EGGDFRDWYKEFNQFGCDVVGISRDSVKSHQNFKTKMDFPFELLSDSDETVCELFGVM
                     KMKNMYGKQVRGIERSTFVIDSNGILRREWRGVKIPGHVEEVLEFVKTLPSERHEL"
     gene            840..2282
                     /locus_tag="VFI43_06225"
     CDS             840..2282
                     /locus_tag="VFI43_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7061759.1"
                     /translation="MSSRKPVSPQSSATTVTRLRAATKLFVLDSNVLMHDPTSLFRFQ
                     EHDVYIPMVTLEELDHNKKGMSEVARNARQTSRFLDEIVSSAIADIDEGISLEPHGTK
                     NATGRLFLQTQAISSVLPMRLASGSADNQIIGVVKFLHETYPNRTVALVSKDINMRIK
                     ARALGLAAEDYFNDKVLEDTDVLFSGVQQLPPDFWDQHGKDMESWQQSGYTFYRIKGP
                     LCADFLTNQFVYVEDDKPFYAQVKECGGGTAVLQTLKDYTHLKNNVWGITARNREQNF
                     ALNLLMNPEIDFVTLLGQAGTGKTLLTLAAGLMQTLEQKVYSEIIMTRVTVPVGEDIG
                     FLPGTEEEKMTPWMGALEDNLDVLNKTEVDAGEWGRAATRDLIRSRIKIKSLNFMRGR
                     TFINKLLVIDEAQNLTPKQMKTLITRAGPATKVVCLGNIAQIDTPYLSEGSSGLTYVV
                     DRFKGWNHSGHITLQRGERSRLADYAAEVL"
     gene            complement(2331..3719)
                     /gene="dnaB"
                     /locus_tag="VFI43_06230"
     CDS             complement(2331..3719)
                     /gene="dnaB"
                     /locus_tag="VFI43_06230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7061760.1"
                     /translation="MVQFRTVAPTDPLLEPYKTPPHSVEAEQSVLGGLMLDNHAWDKV
                     ADILTEDDFYRQDHRLIYRHICKLVEHSKPADVITVAESLELSAELENAGGLPYIGIM
                     AQNTPSAANIRRYAEIVRERSIMRNLAQVGSEISDSAYSPAGRSAADLLDEAEAKVFE
                     IAEAGSRGKQGFVDIQPLLKQVVERIETLYNQDSPSDVTGIATGFPDLDQKTSGFQPG
                     DLIIIAGRPSMGKTAFSLNIAEHVALILQKPVAVFSMEMGGAQLAMRMLGSVGRLDQH
                     KVRTGRLQDEDWPKLTHALGKLSDAPVFIDETAALNALELRARARRLYRQHGELGLIV
                     VDYLQLMSASAQGENRATEISEISRSLKALAKELHVPVVALSQLNRSLEQRPNKRPVM
                     SDLRESGAIEQDADLILFIYRDEVYNPETQDKGIAEIIIGKQRNGPIGKIDLTFLGEY
                     TRFESYAPSGHY"
     gene            complement(3826..4281)
                     /gene="rplI"
                     /locus_tag="VFI43_06235"
     CDS             complement(3826..4281)
                     /gene="rplI"
                     /locus_tag="VFI43_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7061761.1"
                     /translation="MQIILMEKVANLGQLGDVVKVRNGYARNFLIPKGKAKRATQAAI
                     AEFEAKRAELEKNQADVVAAAQSQAEKLNGLMVQVTRKAGIDGKLFGSVTNADIEEAL
                     QAQGITIERAMIRMPQGPLKQVGDHPITIGLHSDVVANIIVSVLGEPTS"
     gene            complement(4293..4577)
                     /gene="rpsR"
                     /locus_tag="VFI43_06240"
     CDS             complement(4293..4577)
                     /gene="rpsR"
                     /locus_tag="VFI43_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7061762.1"
                     /translation="MARFISRRKDTKDKEKDKKLGRPLFKRRKFCRFTAEGIKKVDYK
                     DVDLLKDFINENGKIIPARITGTKSRYQRQLGTAIERARFLALLPYTDLH"
     gene            complement(4605..4901)
                     /gene="priB"
                     /locus_tag="VFI43_06245"
     CDS             complement(4605..4901)
                     /gene="priB"
                     /locus_tag="VFI43_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HET7061763.1"
                     /translation="MGCNDVAICGQIVETSALRYTPAGVPVAEFKITHASRQIEAGRS
                     RQVECEISAVALGQMAEAVSRVETGMLLKLTGFLAKKSRMNPQLILHVNGIDFI"
     gene            complement(4902..5291)
                     /gene="rpsF"
                     /locus_tag="VFI43_06250"
     CDS             complement(4902..5291)
                     /gene="rpsF"
                     /locus_tag="VFI43_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7061764.1"
                     /translation="MRNYEIVFIVHPDQSEQVPAMIERYRGIVTARNGRVHRLEDWGR
                     RQLAYLIQKVHKAHYVLMNIECDQETLDELEHSFKFNDAILRHLTIKMDEPMTAPSPM
                     MREEKSKPAGPAVEDTRETTLAIGPGK"
     gene            complement(5641..6396)
                     /gene="rlmB"
                     /locus_tag="VFI43_06255"
     CDS             complement(5641..6396)
                     /gene="rlmB"
                     /locus_tag="VFI43_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7061765.1"
                     /translation="MSSTRFIFGFHAIVSRLRQNPESIKQVFLDSGRNDQRARDLCNL
                     AEAQGVRLIYCDETRLAGIAGEARHQGVAASIDPTRSHVVIEDVLDTLTEPALLLALD
                     GITDPHNLGACLRVADAFGVHAVIAPKDRAVGLTAAVYKVASGAADTVPYIPVTNLAR
                     TLRELKARAITIIGAAAEAGNSVYSARFEGSIAWVLGAEGKGMRRLTRETCDQIVSIP
                     MQGSVESLNVSVSAGVCLFETCRQRAVLSKHSE"
     gene            complement(6411..8576)
                     /gene="rnr"
                     /locus_tag="VFI43_06260"
     CDS             complement(6411..8576)
                     /gene="rnr"
                     /locus_tag="VFI43_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET7061766.1"
                     /translation="MPIKKKQKLRQLDPYLEREKERYSHALPSREFILEILRERGIPL
                     NEQALRELLQITDDEAAIFDRRLSAMLREGQIIRNRKGDICVVEKLDLIKGRVQGHAD
                     GFGFLIPDDGSGDVFLSAKEMHKVLHGDRVIVRETGVDRRGRREGVIVEVLERAVTQL
                     VGRLHSDHGILFVEAENRRISQDILIPREESMGAAAGQVVMVEIIQQPSKHAQPIGRI
                     TEVLGDYGSPGMEIEIALRKHDLPHQFPLEVEKLSAKFPAEVQKRELKKREDLCHLPL
                     VTIDGETARDFDDAVYCERDEEGYRLFVAIADVSHYVGPGDALDREAFNRGNSVYFPR
                     RVIPMLPEVLSNGLCSLNPQVDRLCMVCEMKIDGAGDFQDYRFFPGVMHSQARLTYTK
                     VAAMLENPRGEDARQYKNILPHIQLLYKLFKVLLKARKKRGAIDFETVETQMVFNDQG
                     KIERIVPIKRNDAHRIIEECMLAANVCASDFLQKHKQPTLYRIHEKPTAEKVAALRDF
                     LKEFGVQLGGGSQPSAKDYARTLSKIKDRPDAQLLQTVMLRSLSQAVYSPDNVGHFGL
                     AYESYTHFTSPIRRYPDLLVHRAIKAVLNEKIYSPGDWHALGMHCSQTERRADDATRD
                     VEAWLKCYYMQDKIGECFDGVISGVVAFGLFVTLNGIYVDGLIHISELPSDYFHFDAG
                     KHMLLGERTGLRYRLGDPVRVKLVRVDLETTKIDFVLAV"
     gene            8634..8720
                     /locus_tag="VFI43_06265"
     tRNA            8634..8720
                     /locus_tag="VFI43_06265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8668..8670,aa:Leu,seq:cag)
BASE COUNT         2043 a         2522 c         2313 g         2139 t
ORIGIN      
        1 aagacacgag cgtcctgaaa ttcctcgact ctctggaaaa ccataaaaca gtcgtgctgg
       61 atgccccccc cacagcggaa aatctcgcgc tcgccgcctt tcatatcctg cattccgcct
      121 acggggacat ctacggcaac cacctgcggc tggaacgagt acgtttattc gaaaccccca
      181 attgctgggc agacgcggta cgggagcact tgccatcagc acggccatat cctgttgttt
      241 ccggcgtata gaaaggaccg gcgccaagcc tcaagacgcc ttgcagtgcc tttactcccc
      301 gtttacttta tgacatgcta acatgcaaaa tgaaaattca gtccccgagt ttccaataag
      361 cgagcgtttc tccatggtaa accaacctgt tcctgatttt gaattgccct cgacggcaaa
      421 aaagaatttt cagctatcag ctgcaacggg caagacactt gtcatttatt tctacccaaa
      481 ggatgacacg cccggctgca cgactgaagg cggagatttc cgggactggt acaaggaatt
      541 caaccagttc ggttgcgatg tcgtcggcat ttcccgggac agcgtaaagt cgcatcagaa
      601 tttcaagaca aaaatggatt ttccattcga actgttgagc gatagcgatg aaacggtgtg
      661 cgagttgttc ggcgtgatga agatgaagaa tatgtatggc aagcaggtgc ggggcatcga
      721 gcgcagcacg tttgttatcg attcgaatgg gattttgcga agggaatggc gaggagtaaa
      781 aatacctggg catgtcgagg aagtgctgga gtttgtaaaa accctgccca gcgaaagaca
      841 tgagctctag aaaaccggtt tcgccacaat catctgctac cacggttacg cgcctacggg
      901 ctgccaccaa gctgttcgtg ctggacagca atgttctcat gcacgacccg acaagccttt
      961 tccgtttcca ggagcatgat gtctatattc cgatggtcac ccttgaagag cttgatcaca
     1021 acaagaaggg gatgtcggaa gtcgcccgta acgcccggca gaccagccgc ttcctcgatg
     1081 agattgtcag cagcgcaata gcggatattg acgagggaat ttcgctggag ccgcacggta
     1141 ccaagaatgc aacgggaaga ctctttctcc agacacaagc gatcagcagc gtgttgccaa
     1201 tgcgccttgc cagcggaagt gcagataacc agattatcgg cgtcgtaaaa ttcctgcacg
     1261 aaacctaccc caaccgcacc gtcgcgctgg tctccaaaga tatcaacatg cgcatcaagg
     1321 ctcgtgcgct ggggttggcg gcagaggatt acttcaacga caaggttctc gaggacactg
     1381 acgtcctgtt ttccggcgtg cagcaactcc cacccgattt ttgggatcag cacggcaagg
     1441 atatggagtc ctggcaacaa tcgggatata ccttttaccg gataaagggg cctctctgcg
     1501 cagacttcct gacgaatcag ttcgtctacg tggaagacga caagccattt tatgcccagg
     1561 tcaaggaatg cggcggggga accgcggttt tacagacgct caaggattac acccacctta
     1621 agaataatgt ttggggcatc accgcgcgta accgcgaaca gaactttgcc ctgaatctgt
     1681 tgatgaaccc cgagatcgac tttgtcaccc tgctggggca ggcgggaaca ggaaaaacgc
     1741 tgttgacact cgcggcgggg ctgatgcaga cgcttgaaca gaaggtgtat tctgaaatca
     1801 tcatgactcg cgtcaccgtt ccggtaggtg aggacattgg attcttgccc ggcacggaag
     1861 aagagaagat gaccccctgg atgggggcgc tcgaagataa tctggatgtg ctcaacaaga
     1921 ccgaggtcga cgcaggtgaa tgggggcgcg ctgctactcg cgatcttatc cgttcccgga
     1981 tcaagataaa gtcactgaat ttcatgcgcg gacgcacttt catcaacaag cttcttgtca
     2041 tcgatgaggc gcagaacctt accccaaaac agatgaagac gcttatcaca cgcgctgggc
     2101 cggccacgaa ggtcgtgtgc ctgggcaata ttgcccagat cgatacgcct tacctgtcgg
     2161 aaggcagttc agggttaacg tacgtggttg atcgcttcaa aggatggaat catagcggac
     2221 atattacctt gcagcggggc gaacgatcca ggcttgccga ctacgcagcc gaggtgttat
     2281 aagggcaggc actcggcgga cggaggcgag acttccggtt cagcagcttc tcaataatgc
     2341 cctgacgggg cgtagctctc gaaacgggtg tattccccaa gaaaggtaag gtcaatcttt
     2401 cctatcggac cgttgcgctg cttgcctatt atgatttcgg caattccctt gtcctgcgtt
     2461 tcggggttat agacttcgtc ccgatagata aaaaggatca ggtccgcgtc ctgttcgatg
     2521 gcccctgact cccgcaggtc ggacatgaca ggccgcttgt tcgggcgctg ttcgaggctg
     2581 cggttcaatt gtgataacgc aaccaccggc acgtgcaatt cttttgccaa cgccttgagc
     2641 gaacgcgata tctcggaaat ctcggtggcc ctgttttctc cctgggccga ggcggacatg
     2701 agctgcaagt agtccaccac gatcaatccc agttcgccat gttgccggta aagtcggcgt
     2761 gcccgagccc ggagttccaa tgcgttgagc gctgccgtct cgtcgataaa cacaggggca
     2821 tcgctgagct ttccgagtgc gtgcgtaagc ttgggccagt cctcatcctg gagacggccc
     2881 gtgcgcacct tgtgctgatc aagccgtccc acagacccga gcatacgcat ggccaactgc
     2941 gcgccaccca tttccatact gaagactgcc accggttttt gcaatatcaa cgcgacgtgt
     3001 tcggcgatat tcaatgaaaa agccgtttta cccatggagg gccggcccgc gatgataatc
     3061 aaatcgcccg gctgaaaccc ggaggttttc tgatccagat cgggaaaccc tgtggcgata
     3121 ccggtaacat cgctggggct atcctggttg taaagcgttt cgatgcgctc gacgacttgc
     3181 ttcaggagcg gttgaatatc gacaaatccc tgctttccgc gggagcccgc ctcggcaatt
     3241 tcgaacacct tggcttcagc ttcatcgaga agatctgctg ctgagcgtcc cgcaggactg
     3301 tatgcggagt cggaaatttc ggaaccgacc tgcgccaaat tgcgcatgat ggaccgctcg
     3361 cgcacgatct ccgcataacg gcggatattg gctgccgaag gcgtgttctg ggccatgatg
     3421 ccaatgtaag gcaaacctcc cgcattctcc agttcggctg acagttcaag tgattccgct
     3481 acggttatga catccgctgg cttgctgtgc tcaaccagct tgcagatatg gcggtaaata
     3541 aggcggtggt cctgccggta gaaatcatcc tccgtgagaa tatccgccac cttatcccac
     3601 gcatgattgt ccagcatcag cccgcccagc actgactgtt ccgcctccac agagtgcggc
     3661 ggggtcttgt acggttcaag caacggatcg gtgggagcta cagtgcgaaa ttgaaccatg
     3721 agatgcgacc gggaggaatc gggaagattc taccattgtt ggtggccgga aaaacaaaaa
     3781 gggactgttg agtccaacag tccctctctt tcttgttcga cccactcagg aagtgggttc
     3841 gcccagaacc gacacgatga tattagccac gacgtcgctg tgcaatccaa tcgtgattgg
     3901 atggtcaccc acctgtttca gaggcccttg cggcatccgg atcatcgctc gctctatggt
     3961 tatcccctgt gcctgcaacg cctcctcgat gtcggcattg gttaccgaac caaacagctt
     4021 gccgtctatc cctgctttac gggtcacttg aaccatcaac ccgttcagtt tttccgcctg
     4081 gctctgtgcc gcagccacaa catccgcctg gtttttttca agttccgcac gcttggcctc
     4141 gaattctgct atggctgcct gggtcgcccg cttggcctta cccttgggaa tcaggaaatt
     4201 ccgcgcataa ccgtttctaa ccttgacgac atcgcccaac tgaccgaggt ttgccacttt
     4261 ttccatcaat atgatttgca tgtcaacatc tctcagtgca ggtcagtgta aggaagcaaa
     4321 gcgagaaacc gcgcgcgttc gatggcggtg ccgagctgac gctgataccg ggattttgtt
     4381 ccagttatcc gggccgggat gattttgccg ttttcgttta tgaaatcttt cagcagatca
     4441 acatccttgt agtccacttt cttgatgcct tcggcagtga aacggcagaa tttgcgccgc
     4501 ttgaagagcg ggcgtcccag tttcttgtcc ttctccttgt ccttggtgtc ttttcgtctt
     4561 gaaataaagc gagccataat tttctacctc ttgaataaat acggttagat aaagtcgatt
     4621 ccgttcacgt gcaagatgag ttgaggattc atgcggcttt tcttggcgag aaaaccagtc
     4681 aatttgagta acatgcctgt ctccacccgc gaaactgcct cagccatttg ccccaatgcc
     4741 actgcggaaa tttcgcattc gacctggcgg gaccgtcccg cctcgatctg acgggaggca
     4801 tgggtaatct tgaactcggc taccgggacc ccggccgggg tgtagcgaag agcgctcgtt
     4861 tcgacaattt gtccacaaat cgcaacatcg ttgcagccca attacttccc tgggcctatg
     4921 gcaagcgttg tctctcgcgt gtcttctacc gcagggcctg ccggcttgct tttctcttca
     4981 cgcatcatgg gagaaggcgc ggtcattggc tcgtccattt tgatggtgag atgccgtaat
     5041 atcgcgtcgt tgaacttgaa ggaatgctcc agctcatcca atgtttcctg atcacactca
     5101 atattcatca atacatagtg cgctttgtgg actttttgta tcagataggc aagctggcgg
     5161 cgcccccagt cctcgagtcg atgaacccga ccattcctcg cggtaacaat gccgcggtaa
     5221 cgctcgatca tcgcgggaac ttgctcgctc tgatccgggt gaacaataaa gacgatttcg
     5281 taatttcgca tgcaattttc cttatgggta aagcctcccg cggtgcaagc gcggtgaggc
     5341 aagggttgaa ttggagttcg tcaggatacc agggcgggct ccaaggagga atgcaagcgt
     5401 aaattttctt gcgccatgcc gccttcaatt tgccctaatt taccccatga gcctttacga
     5461 cgagttatcc agattgagga ggtcggtact atactgaaag ggaaaggaaa tatcaattgt
     5521 atcaactggt cccgcaaata ttcgcgacac gcacactcat tctcaccttg ccgcttccgc
     5581 ctgtgtagca gtacttgctg gccctttcgc ccaatcgaga tttttttgtc attccttctg
     5641 tcattccgaa tgtttgctta atacagcacg ctgtcggcag gtctcgaaga gacatactcc
     5701 ggcgctcacc gagacgttaa gactctccac gcttccctgc atcggaatgg aaacaatctg
     5761 gtcgcatgtc tcgcgcgtca gccgccgcat gccctttccc tccgccccca gaacccatgc
     5821 gatggaacct tcaaacctag cggaatagac gctgttgccc gcttcggcag cagccccgat
     5881 tatcgtgatt gcacgcgctt tgagctccct aagggtgcgt gcgagatttg ttacgggtat
     5941 atacggcact gtatccgctg caccactcgc aaccttatat accgctgcgg taagtccgac
     6001 agcgcgatcc ttgggcgcga tcactgcatg aacgccaaat gcgtccgcga cgcgcaggca
     6061 ggccccgaga ttgtgcggat ccgtaatgcc atccagcgcc aggagcagcg ccggttcggt
     6121 cagcgtatcc agcacgtctt cgatcacgac gtggcttcgt gtaggatcaa tggaggcggc
     6181 caccccttga tggcgagcct cgcctgcaat gcctgcaagc cttgtctcgt cgcaataaat
     6241 cagacgtact ccttgagctt cggcgaggtt acacaaatcg cgtgcgcgct gatcattgcg
     6301 cccgctgtcg agaaaaacct gcttgatact ctcggggttt tgacgtagcc ggcttacgat
     6361 tgcgtgaaat ccgaaaatga aacgggtgct ggacatctcg ggaaccaagc ctacacggct
     6421 agcacaaaat cgatcttggt ggtttcaagg tcgacacgca ccagttttac tcggacagga
     6481 tcccccaaac ggtagcggag accggtgcgt tcgccgagga gcatatgttt tccggcatcg
     6541 aaatgaaaat aatcgctagg cagttcggaa atatggatga gcccgtcgac gtaaattcca
     6601 ttcagggtga cgaacaatcc gaaagcaacg acaccgctga tgactccgtc aaagcattca
     6661 ccgatcttgt cctgcatgta ataacatttg agccatgcct caacatctcg cgtcgcatca
     6721 tcggcgcggc gctcggtctg ggaacaatgc attcccaacg catgccagtc tcctggagaa
     6781 tatatcttct cattcaacac ggccttgatg gcacggtgaa ccaatagatc cggatagcgc
     6841 ctgataggtg aagtgaaatg cgtgtaggat tcgtacgcca gtccgaaatg gccaacgtta
     6901 tcggggctgt agaccgcctg gctcaacgaa cgcagcatca cagtctgtaa cagctgcgcg
     6961 tccggcctgt ccttgatctt gctcagcgtc ctggcgtaat ccttggcgct tggctggctt
     7021 cctcctccaa gctgcacgcc gaactccttt agaaaatcac gcagcgccgc caccttctca
     7081 gcagtgggtt tttcatgaat tcggtatagc gtgggctgct tgtgtttctg caaaaaatct
     7141 gaggcgcaca cattggccgc cagcatgcat tcctcgataa tacgatgggc atcattccgc
     7201 tttattggaa caatgcgctc gattttccct tggtcgttga acaccatctg cgtttcaaca
     7261 gtttcaaaat cgatcgctcc ccgcttcttt ctcgctttca gcaaaacctt gaataactta
     7321 taaagaagct gaatatgggg gagaatattc ttatattgcc tggcatcttc gcctcgagga
     7381 ttttccagca tggcggccac cttggtatag gtaagccgtg cctgggaatg cataaccccc
     7441 ggaaaaaagc gataatcctg aaaatcgccc gcaccatcga ttttcatttc gcaaaccatg
     7501 cacaaacggt cgacctgtgg gttaagcgaa cacaatccgt ttgatagcac ttctggcagc
     7561 atcgggatga cgcgccgggg aaaatatacg gaattgccgc gattgaacgc ctccctgtcg
     7621 agcgcgtccc ctggacccac atagtggctg acatcggcaa tagcaacaaa taaccggtat
     7681 ccctcctcat cacgctcgca atatacagcg tcgtcgaagt cccttgccgt ttctccgtct
     7741 atcgttacga gcggtagatg gcaaaggtcc tctcgcttct tcaactctcg cttttgtact
     7801 tcagcgggaa atttcgccga aagcttttca acttccaggg ggaactgatg aggcaggtcg
     7861 tgtttgcgaa gagcaatctc gatttccatt cccggggagc cataatctcc aagaacctcg
     7921 gtaatgcggc cgataggctg agcatgcttg cttggctgct ggatgatttc caccattaca
     7981 acctgtcccg ccgctgctcc catcgactct tcccttggga tgagaatatc ctggctgatg
     8041 cgcctgttct ccgcctccac gaacaggatt ccatgatccg agtgaagtct tcccacgagc
     8101 tgggttacag cgcgctcgag cacctccacg atcacgcctt ccctgcgccc gcggcggtcc
     8161 acccctgttt cgcgtacgat gacgcggtcg ccatgcagta ctttgtgcat ttccttggcg
     8221 cttagaaaca cgtcgccgct tccgtcgtca ggaatcagaa atccgaagcc gtcggcatgc
     8281 ccctgaaccc tccccttgat gaggtccagc ttctccacga cgcaaatatc acccttgcgg
     8341 ttacgtatga tctgaccttc gcgtagcatc gcggaaagcc gtcgatcaaa aatggccgct
     8401 tcatcgtcgg taatttgcaa cagctcccgc agcgcttgct catttaaggg gattccccgc
     8461 tcgcgcagaa tttcaaggat gaattcgcgg ctgggaaggg catgagaata tcgctccttt
     8521 tcccgctcga gataaggatc cagttgacgg agtttttgtt tcttcttgat tggcaaagca
     8581 ggtttcctat agaattgcgg ctccgtccaa acgcgggacg gcacgccctc actgcccaga
     8641 tggcggaatt ggtagacgca ctaggttcag gtcctagcgg tggcaacact gtggaggttc
     8701 gagtcctctt ctgggcacca ccctacacga ttcatccgca ttgctcgcat gcgacatgaa
     8761 acgttcaata actgtgaccg ttgaattata gcagtaccag cataaccgtc cgttgtattg
     8821 gacgtccggc tgctcaactc catgatacct tccgctccaa acgcgtaacg gcacagccat
     8881 gagcgcgacg tttgcgttca gttgtcctga ttccccgggc gtcacgtttc ttgcccgcga
     8941 aaccttttcc gcaaattgaa gaccacaacg ttcgtatcat aaccatttga agactacaac
     9001 gttcgtatca taaccat
//
LOCUS       DASSHJ010000162         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1518378,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000162 DASSHJ010000000
VERSION     DASSHJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518378"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VFI43_06270"
     CDS             complement(<1..1753)
                     /locus_tag="VFI43_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7061767.1"
                     /translation="MGEKRMHKTTLARWLLSGEWKAHRVQAAVAIIAISLGVALGFAI
                     HLINSAAFNEFSAAGRSISGQSDLQVRSPRSTFDEALYPLLAQHPDVELINPVLELDV
                     AVPGKSDERRPATLKILGLDIFHASAMAPDLIGIPGEDKLFDVLADDAIFLSPAAMEW
                     LGVKQGDVLPLRVGTQTVTLRVAGGLRRARAGQRLGVMDIASAQWRFNRLGQLSRIEL
                     KLMPGVNREAFKAKLEQQLETRGQYVITETADQEARVANMSRAYRVNLNMLGMVALFT
                     GTFLVFSTQALSVVRRRHQFALLRVLGLTRNQLLAQVLFEGMVLGVIGSLAGLALGYA
                     TASAALNYFGGDLGGGFFPGVKPSVHFDPWAALAFFTLGLGITLLGSAVPAWEAASTQ
                     AAPALKSGSEEAAMARLGTPWPGILCLASGILFTQLPPVSGLPVFGYLAVAQLLVGGI
                     ALMPRLSAMIFTWVFSLIARRCSATAPALATVALARLANSPNQAAIALGGVLASFTLI
                     VAMGLMVASFRVSVDDWLKHILPADLYVRTAASGDARGLTPDEMTSLAHTPGVIRADF
                     LRSSQLMLDPTRPSVTLI"
     gene            complement(1746..2456)
                     /locus_tag="VFI43_06275"
     CDS             complement(1746..2456)
                     /locus_tag="VFI43_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061768.1"
                     /translation="MLELRHISKAYPNGRSVLSDLSYTLSRGEYVAIMGESGVGKSTL
                     LNLIAGLDAPDAGEILIGGTAISSLSDDAATRVRRRDLGFVFQAFHLLPHLSLSQNIA
                     LPLLLNNISSDQLMERTLQMLDEVGLGGREDHFPRQLSGGELQRVAIARALVHRPKLV
                     LADEPTGNLDPDTAQDILRLMRSEIKANAASGIMVTHSHTAAATADRVLVLRKDGLHP
                     EVSGIQAQWYENPKEADG"
BASE COUNT          618 a          789 c          803 g          565 t
ORIGIN      
        1 caatcagggt cacggaaggg cgggtcggat cgagcatcag ctgcgaagag cgtaaaaagt
       61 cagcgcggat gacaccgggc gtgtgggcga gcgacgtcat ctcatccggc gtaaggcccc
      121 gcgcatcgcc gcttgctgct gtccgtacgt agaggtccgc ggggagaata tgcttcaacc
      181 aatcatccac cgatacgcgg aaactcgcga ccatgagacc catcgcaacg atgagggtaa
      241 aactcgcgag cacgccgccc agggcgatgg cagcctggtt tggcgaattc gccagccgtg
      301 caagcgccac ggtcgcaagc gctggcgcgg ttgcggagca gcgtcgcgca atcagcgaaa
      361 atacccaggt aaatatcatc gcggaaaggc gtggcatgag cgcaataccc ccgaccagca
      421 gttgtgctac cgcgagataa ccgaagaccg gcaagcccga tacgggcgga agttgcgtaa
      481 agagaatccc cgaggcaagg cacaggattc cgggccaggg agttcccagt ctggccatcg
      541 cggcttcttc actcccggat ttgagtgcgg gggcagcttg cgtgcttgcc gcttcccacg
      601 caggaaccgc actccccagc aaggtgatgc caagcccgag agtgaagaag gccagggctg
      661 cccacgggtc gaaatgcacg ctcggcttca cgcccgggaa aaatccgccc cccaggtctc
      721 ctccgaaata attcagcgcc gctgacgccg tcgcatagcc gagggcaagt cctgcgagcg
      781 acccaataac gccgagaacc attccttcga acagaacctg cgcaagtagc tgattacgtg
      841 tcaaccccag cacgcgtagc agtgcgaatt gatggcggcg ccggacgacc gataacgcct
      901 gggtcgaaaa gacaaggaat gttccggtaa acagcgctac cattccaagc atgttgagat
      961 taacccgata ggcacgagac atgttcgcca ctcgagcctc ctgatcggca gtttccgtaa
     1021 ttacgtattg cccgcgtgtt tccagttgtt gctccagctt tgccttgaac gcttccctgt
     1081 taactcccgg catcagtttc agttcgatgc gtgacaattg tccaagccgg ttaaagcgcc
     1141 actgggcgga ggcaatgtcc atcactccca gtcgctgtcc cgcccgggct cgcctcaagc
     1201 cgcctgccac gcgcagggtg acggtctggg ttccaacccg cagcggtaat acgtcgccct
     1261 gcttgactcc caaccattcc attgctgcgg gagaaaggaa aatggcgtcg tccgccagca
     1321 cgtcgaacag cttgtcttct ccagggattc cgatcaggtc gggggccatg gccgaggcgt
     1381 ggaaaatatc cagcccgaga attttcagcg ttgcggggcg gcgctcgtct gattttccag
     1441 ggacggcgac atcgagttcc aggacagggt tgattagttc cacgtcgggg tgctgagcaa
     1501 gaaggggata taacgcttcg tcaaaagttg atcgcggact gcgaacctgc aggtccgact
     1561 ggcccgatat gcttcgtccg gcggcagaaa actcattgaa cgcagcggaa tttataaggt
     1621 gtattgcaaa gccgagggca acgcccagcg aaatcgcaat gatcgccact gcggcctgca
     1681 cccggtgtgc tttccattct cccgagagga gccagcgagc caacgtcgtc ttgtgcatcc
     1741 tcttctcacc catccgcttc tttagggttt tcgtaccatt gcgcctggat accagatacc
     1801 tctggatgta atccatcttt tcgtagcacg agcacgcggt cggcagtggc ggcagccgta
     1861 tgggaatgcg tcaccatgat gccggaagca gcattcgcct ttatttccga acgcatgagc
     1921 ctgagtatgt cctgggcggt atcgggatcg agattgccgg tgggttcatc ggcgagaacc
     1981 agttttggcc ggtgcaccag tgcccgtgcg atggcgaccc gttgcagttc tccgcccgat
     2041 agctggcggg gaaaatggtc ttccctgcca ccaaggccga cctcatccaa catctgcagt
     2101 gtgcgttcca taagctggtc agaggaaatg ttattcagca gcagcggcag ggcgatattc
     2161 tggcttagcg aaagatgcgg caggaggtga aacgcctgga agacaaatcc taaatccctg
     2221 cggcgcacgc gcgtggccgc gtcgtcgctc aaagacgaga tggccgttcc cccgatcaat
     2281 atctctcctg catccggagc gtccagaccc gcaatgaggt ttagcagtgt ggatttgccg
     2341 acccctgatt cacccatgat cgctacgtac tcgcctcgcg aaagggtata ggataaatcc
     2401 gaaagcacac tcctgccgtt ggggtaggcc ttactgatat ggcgcagttc gagcatgaag
     2461 ttctaccaga tgaggagcgt cgttatgccc atgccgccgt tctgacaggg tatggtggtc
     2521 cagggttgat gagcaaattt gggcaaatta tggctattgt acctgctgcc tgttcctgag
     2581 gtaccttact ctgatactgg tagggccttt agagtgatga tcaagagctt ccggacagct
     2641 cctgagcttt acgtctcctt gctgccataa atgttctaat ctgatagaaa gggattcatg
     2701 taggacgttc gagagcataa gctcgacttt tcgataagac cgggactata tggaattcaa
     2761 aaggaagaaa tccat
//
LOCUS       DASSHJ010000163         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_202670, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000163 DASSHJ010000000
VERSION     DASSHJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202670"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFI43_06280"
     CDS             <1..499
                     /locus_tag="VFI43_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7061769.1"
                     /translation="FEIMLPASEAGQFWNALLAAGVAPAGLGARDTLRLEAGMNLYGQ
                     DMDETTNPLESGLGWTIDLKTDREFVGRQALLEKPVTKQLIGLVLLDRGVLRSHQKVT
                     NQDEGEGEITSGGFSPTLNQSIALARMPASVSAGDEVQVIVRDKKLKAKAVKYPFVRN
                     GKILV"
     gene            550..939
                     /gene="gcvH"
                     /locus_tag="VFI43_06285"
     CDS             550..939
                     /gene="gcvH"
                     /locus_tag="VFI43_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET7061770.1"
                     /translation="MSIPDELRYAKSHEWAKLESDGSVTIGITQHAQELLGDMVFVET
                     PEIGRKLAQGEECAVVESVKAAADVYSPIAGEVLAVNPDLELTPEKINQDAYAAWLFK
                     LKPVDAADLSQLMRAAEYQKMLESEDH"
     gene            1063..2421
                     /gene="gcvPA"
                     /locus_tag="VFI43_06290"
     CDS             1063..2421
                     /gene="gcvPA"
                     /locus_tag="VFI43_06290"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET7061771.1"
                     /translation="MPFIPHTEEDVSAMLASIGANSIDDLFDEIPPALKSDELEGVPA
                     GLNEMEITRLMMERAEADGRYLNFIGAGAYEHHIPAAVWQIATRGEYYSSYTPYQAEA
                     SQGTLQLLYEFQTMMASLTGMDVSNASMYDGASALAEAALMAVRSHKSSRRILMPRTI
                     HPVYRSVVQAIVRSQGIEVVDVPYCRDSGRTQVEALDAIGDGEFAALVIPQPNFFGVL
                     EDVDILTDWAHRKHSLAIGVVNPMALAILTPPGNWGEKGADVAVGEGQPLGIPLSSGG
                     PYFGFMACKQALVRQMPGRIIGRTTDSEGKPGFVLTLQAREQHIRRSKATSNICTNQG
                     LMVTAATIYMALMGPEGLRRTAAQSHANTLALLEKLESLKGVERIFNGPVFDELAISL
                     PAPVQNVLGELRAAGILGGLNLEQYYPELGSSMLVCATETKSPADLTTYADKLSQILD
                     RF"
     gene            2476..2988
                     /gene="tpx"
                     /locus_tag="VFI43_06295"
     CDS             2476..2988
                     /gene="tpx"
                     /locus_tag="VFI43_06295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET7061772.1"
                     /translation="MVTLAGNPIKVEGTFPKVGDRAPDFSLVNKDLKDVTLADFAGKK
                     KVLNIVPSLDTPVCAVSTRKFNEKASNMENTVVLIIAADLPFAMSRFCDTEGLDKVVI
                     LSTMRGAEFMKNYGVAITDSPLAGITARAVLVLDENDKVIHAQLVPEIKDEPDYDAVM
                     AALEPASAPA"
     gene            3070..>4208
                     /gene="gcvPB"
                     /locus_tag="VFI43_06300"
     CDS             3070..>4208
                     /gene="gcvPB"
                     /locus_tag="VFI43_06300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET7061773.1"
                     /translation="MLIFEHSRPGRLNRSQLPETEEIKDIPANLVRKAPPLLPEVSEM
                     DAVRHYTRLSQLNFSIDTQFYPLGSCTMKYNPRACNSLAMLPQYLSRHPRAPESTGQG
                     FLACMYELQEILKAVTGMAGVSLTPMAGAQGELAGISMIRAYHEARGDTARTEIIVPD
                     AAHGTNPATAVMCGYKVVEIPTDKEGDVDMEALKAAVGPQTAGLMLTNPSTLGVFEKN
                     IAEMSKLVHQAGGLLYYDGANLNAILGKVKPGDMGFDVIHINLHKTFSTPHGGGGPGS
                     APVGVAERLLPFMPVPVVAFENGQYRWLTETDLPKSIGRLSAHMGNAGVLLRAYVYVR
                     LLGSEGMHRVAEFAALNANYLMAELSKAGFEIAYPTRRASHEFIVT"
BASE COUNT          987 a         1108 c         1196 g          917 t
ORIGIN      
        1 ctttgaaatc atgctgcccg cttccgaggc aggccagttc tggaacgcct tgcttgcagc
       61 gggtgtcgcc cccgcgggcc tcggcgcgcg cgatacgtta cggttggaag caggcatgaa
      121 tctctacggg caggatatgg atgaaaccac caacccactt gaatccggac tcggctggac
      181 gattgatctg aaaacagatc gtgaatttgt cggtcggcag gcactccttg agaaaccggt
      241 cacgaaacaa ctgatcggac ttgtgttgct tgaccggggc gtattgcgca gccatcagaa
      301 ggtgaccaac caggacgaag gtgaaggaga gattaccagt ggaggctttt ctccgacgct
      361 gaaccagtcc atcgccctgg cgcggatgcc cgcctctgtt tcggcagggg atgaggtgca
      421 ggtgatcgtc agggacaaaa aactgaaagc aaaggcggtg aaatatccgt tcgtgagaaa
      481 tggcaaaata ctggtctgat tcttttttaa ccacaagatg ccgttattta attattctgg
      541 agtaaaaaca tgagtattcc tgacgagttg aggtacgcaa aatcccatga atgggcgaaa
      601 ctggaaagcg atgggtcggt aacaataggt atcacgcagc atgcgcagga attgctgggc
      661 gatatggtat tcgtggaaac gccggagata ggacgcaaac ttgcccaggg ggaagaatgc
      721 gcggtggttg agtcagtgaa agccgccgcc gacgtatatt ctcccatcgc tggagaagtg
      781 cttgcagtga atcccgatct tgagttgacg cccgagaaaa tcaatcagga cgcgtatgcg
      841 gcatggctgt tcaagttaaa gccggtcgac gcggccgacc tgagccagct catgcgtgca
      901 gcagaatatc agaagatgct ggaaagcgag gaccactagc gcctgctcag cgaaccggca
      961 tccggaagcg cgttcacttt cacgtctacg cgctcctttt ctcccgctaa tttttctacc
     1021 ccgattatta ttttttgatg agcagaaact acggaacaag acatgccgtt cattccccat
     1081 actgaagaag atgtgagcgc gatgcttgca agcatcggcg caaattcgat cgatgatctg
     1141 ttcgatgaaa tcccccctgc tctgaaaagt gacgaactgg agggagtgcc cgcgggattg
     1201 aatgaaatgg aaatcacgcg gttgatgatg gaaagggcag aagccgatgg ccgctatctc
     1261 aacttcattg gtgcgggcgc gtatgagcat cacattcctg ccgccgtgtg gcagattgca
     1321 acgcgtggag aatactattc ctcctacacg ccctatcagg cggaggcgag ccagggaacg
     1381 cttcagcttc tgtatgaatt ccagacgatg atggcttcgc tgaccggaat ggatgtatcc
     1441 aacgccagca tgtacgatgg ggcctccgcg cttgcggaag cggctctgat ggcggtgcgc
     1501 tcgcacaagt cgtcgcgccg cattctcatg ccccgaacga ttcatccggt ttatcgcagc
     1561 gtcgtgcagg caatcgtgcg cagccaggga attgaagtgg tcgacgttcc ctattgccgg
     1621 gattcaggtc gcacccaggt ggaagcactg gatgcaatcg gcgatggcga attcgctgcc
     1681 ctagtcattc cccagcccaa tttctttggc gttctcgagg atgttgatat tctcaccgat
     1741 tgggctcatc gcaaacactc gctggcgatt ggcgttgtca atcccatggc gctcgcgatc
     1801 cttactcccc ctggcaattg gggagaaaaa ggcgcagatg tggccgttgg agagggccaa
     1861 ccattaggca tacctctttc cagcggcgga ccttattttg gctttatggc ttgcaagcag
     1921 gcactggtgc gccagatgcc cggacggatt atcggtcgca cgacggattc cgagggcaaa
     1981 cctggctttg tgctgacgct gcaggctcgt gaacagcata tcaggcgctc caaggcgacc
     2041 tccaacattt gcacgaacca ggggttgatg gtcacggcgg cgacaattta catggcgcta
     2101 atgggaccgg agggattgcg gcgcactgct gcccaatccc acgccaacac gcttgccttg
     2161 ctcgaaaagc tggagagcct gaagggggtg gaacggattt tcaacgggcc cgttttcgac
     2221 gagcttgcta tttcactgcc cgcccctgtg cagaacgtgt tgggcgagct tcgtgcggct
     2281 gggattctcg gtgggctgaa cctcgaacag tattatccgg agctcggcag ttcgatgctg
     2341 gtatgcgcga ccgaaacaaa aagcccggcg gatttaacga cctatgccga taaattgtcg
     2401 caaatacttg atcgtttcta atatttcccg cgtttttcat tgatggtcat attcacccac
     2461 acttaaggag gacgaatggt tactctcgca ggcaatccca ttaaggtaga aggaaccttt
     2521 cccaaggttg gcgaccgtgc gccggatttt tcgcttgtca acaaggatct gaaggacgtg
     2581 actcttgcgg atttcgccgg gaagaaaaaa gtgctgaata ttgtccccag cctggacact
     2641 ccggtttgtg cggtctctac ccgcaagttc aacgagaaag ccagcaacat ggaaaatacc
     2701 gtggtgctga tcatcgcagc tgatctaccg ttcgcaatga gccggttttg cgatactgaa
     2761 gggctggaca aagtggtgat tctctcaacc atgagaggag ccgagttcat gaagaactat
     2821 ggggttgcga tcaccgatag tccgctcgcg ggaatcaccg cccgcgctgt gctggtactg
     2881 gatgaaaatg acaaggtgat ccatgctcaa ctggtgccgg aaatcaagga cgaacctgac
     2941 tacgatgccg tgatggctgc tctcgagcct gcctccgccc ccgcctgatc gcgaaggcca
     3001 cagcctgtta aaacgctcac tcaggctgtg gcaccccaaa cactgataac cgccaaattg
     3061 aattgacata tgttgatatt tgaacactcg cgtcccggcc ggcttaaccg ttctcaacta
     3121 cctgaaactg aggaaataaa agatattccc gctaacctgg tgcggaaggc gccaccgctt
     3181 cttccggaag tatccgaaat ggacgccgta cgtcactaca ccaggctgtc gcagttgaat
     3241 ttttcgatcg acacgcaatt ttatcccctt ggctcgtgca cgatgaaata caaccctcgg
     3301 gcgtgcaatt ctctcgcgat gctgcctcaa tacctgtcac ggcacccgcg cgcgcctgaa
     3361 agcacgggac aggggtttct tgcatgcatg tatgaattgc aagagatatt gaaggcagtc
     3421 acggggatgg caggtgtcag cctgaccccc atggccggcg cccagggcga actggctggc
     3481 atatcgatga tccgcgccta ccacgaagcg cggggagata ctgcccgaac cgagatcatc
     3541 gttcccgacg cggcccatgg gacaaacccg gccaccgcgg tgatgtgcgg ttataaggtc
     3601 gtcgaaatac cgacagacaa ggaaggcgac gtcgacatgg aggccttgaa ggcggcagtg
     3661 gggccacaaa cggctggatt gatgctaacc aatccatcga cacttggcgt attcgaaaaa
     3721 aatatcgccg agatgagcaa gctggtgcac caggccggcg ggcttttata ttatgacggg
     3781 gccaacctga acgccatact gggcaaggta aagcctggag atatgggctt cgacgtgatt
     3841 cacatcaatc tgcataagac tttctccact cctcatggtg gtggaggtcc gggttcagca
     3901 ccggtcggag tcgcggagcg cttattgcca tttatgccgg taccagtggt ggcctttgaa
     3961 aatggccaat atcgctggtt gaccgaaaca gacctcccga aatcgatagg caggctttct
     4021 gcgcatatgg gtaacgccgg cgtgttgctt cgcgcgtatg tgtacgtacg cctgctgggc
     4081 tcggaaggca tgcaccgcgt agccgaattc gccgctctca acgctaatta cctgatggcg
     4141 gaactgagca aagcaggctt tgaaatcgcg tatccgacac gccgcgcaag ccatgagttt
     4201 attgttac
//
LOCUS       DASSHJ010000164         6812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1098337,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000164 DASSHJ010000000
VERSION     DASSHJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098337"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VFI43_06305"
     CDS             <1..959
                     /locus_tag="VFI43_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7061774.1"
                     /translation="DPNKLMVGEWVVAIGSPFGFENSVTAGIVSAKGRSLAQENFVPF
                     IQTDVAINPGNSGGPLFNLKGEVVGINSQIYSRTGGFMGLSFAIPIDVAMDIVNQLIA
                     TGKVSRGRIGVMIQEVTKELAESFGLSKPAGALVASVQKGGPAEKAGIEPRDVILKFD
                     GKPVNASGDLPRIVAATKPGSKVQVQVWRNGATKDITVTVDELPPDEKPGSRGGKRGK
                     ATPDTSNRIGLMLSELSPDQKKQLEVENGLLVEDMVPGVGSRAGLRPGDVILSINNQD
                     VSSVDQFNQLLNKIPKGRNIALLIRRGDATTFITMKVNGDNK"
     gene            943..1230
                     /locus_tag="VFI43_06310"
     CDS             943..1230
                     /locus_tag="VFI43_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET7061775.1"
                     /translation="MATTSNPSPPLSSANPPELVVYSREGCHLCEDMISALQQWQERE
                     RFSFEIVDVDSDQNLRLRYGERVPVLIAGETEVCHYHLDPLEFGKLFSSPS"
     gene            1476..3272
                     /gene="lepA"
                     /locus_tag="VFI43_06315"
     CDS             1476..3272
                     /gene="lepA"
                     /locus_tag="VFI43_06315"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET7061776.1"
                     /translation="MMLHIRNFSIIAHIDHGKSTLADRIIQMCGGLSDREMEAQVLDS
                     MDLERERGITIKAQTAALEYRSRDGNQYLLNLIDTPGHVDFSYEVSRSLAACEGALLV
                     VDASQGVEAQTVANCYTATEQGVEVVPVLNKIDLPAADPARVIKEIEDIIGIDAQDAL
                     YASAKTGQGVDEILEAVISRIPPPKGNPAAPLKALIIDSWFDNYVGVVMLVRVFDGVL
                     KPKDKILLMASKAVYLCEQVGVFTPKSRNRESLSAGEVGFIISGIKELKSAKVGDTIT
                     LADRPAEQPLLGFKEIKPQVFAGLYPVESNQYDALRDALEKLKLNDSSLHYEPETSQA
                     LGFGFRCGFLGLLHLDIVQERLEREYGMDLITTAPTVIYQIVLRDGSVIEIENPSRMP
                     DPSKIQEIREPIITATILVPHEYVGSVITLCISKRGAQKNMQYMGRQVMLTYEMPLNE
                     VVMDFFDRLKSVSRGYASLDYEFKEFRASDLVKLDILINGEKVDALSLIVHRASSQYR
                     GRELAERMRELIPRQMFDIAVQAAIGSHIIARESIKALRKNVLAKCYGGDITRKRKLL
                     EKQKAGKKRMKQVGNVEIPQEAFLAILKVGEK"
     gene            3356..4159
                     /gene="lepB"
                     /locus_tag="VFI43_06320"
     CDS             3356..4159
                     /gene="lepB"
                     /locus_tag="VFI43_06320"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7061777.1"
                     /translation="MNFPLIMFILLLVTGGIVLLDRFVLQPRRGAGTEGPWWVEYPRS
                     FFPVILIVFCLRSFLVEPFKIPSGSMIPTLLVGDFILVNKYTYGIRLPVINMKILDMK
                     EPQRGEVMVFRYPENPSMDYIKRVIGIPGDSVSYRDKQLFINNSPVKMEPDGEYTYVE
                     SGLAYVYTKRFNESFGGPDHKILINPELPDLMLAGVRRFPLQENCTYDDTGFTCKVPA
                     GNYFTMGDNRDSSSDSRYWGFVPEKNIVGKAFLIWWNFSDLKRVGTSIE"
     gene            4253..4927
                     /gene="rnc"
                     /locus_tag="VFI43_06325"
     CDS             4253..4927
                     /gene="rnc"
                     /locus_tag="VFI43_06325"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7061778.1"
                     /translation="MNNDTLCRKIGYAFRQPEFLRQALTHRSHSLPHNERLEFLGDSV
                     LNCAVATLLFRHFPDRAEGDLSRLRANLVNQQALADIAQMLDLGKLVRFGEGEIKSGG
                     NRRPSILADALEAVLGAIYLDGGFAAAEKVITTLYTPLLSELNTDTPGKDPKSLLQEY
                     LQSRHLAPPRYAVIATTGEAHQQQFEVECVISKLNMRTLGEGASRRSAEQEAARAAYR
                     QLHSQT"
     gene            5040..5951
                     /gene="era"
                     /locus_tag="VFI43_06330"
     CDS             5040..5951
                     /gene="era"
                     /locus_tag="VFI43_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7061779.1"
                     /translation="MTNAGDHITDSGYRCGLIAIVGRPNVGKSTLLNRLVGQKISITS
                     RKSQTTRHRILGIVTRPESQFIFVDTPGFQSQHGNRLNAIMNRTVQQSVHEVDVVVFV
                     IEALHFDERDRRVMKLLPAGKPVILAINKIDRLADKAPLLPFIQKMALELNPTTVVPV
                     SAERGTQLDNLLSAIRPYLPEQPALFGNDEVTDRSERFIAAELVREKLFRLLGEELPY
                     STTVIIDQFSTEGELRKIHASVIVDKPGQKAIVIGQNGEKLKLIGTSARKDMEEVFGG
                     KVYLEIWVKVKSGWADDVHALKSFGYE"
     gene            6006..6728
                     /gene="recO"
                     /locus_tag="VFI43_06335"
     CDS             6006..6728
                     /gene="recO"
                     /locus_tag="VFI43_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7061780.1"
                     /translation="MSVDKQDAQPAFILHSYPYSETSLIVEAFTRYSGRLPLLAKGAK
                     RPRSALRGMLRAFQPLQLSWGGKSELRVLHKAEWQGGFIPLQGTGLICGFYLNELLMR
                     LLHRNDPHEQLFVHYQDALAALSEHSDTISILRKFEQRLLRELGYAVTLDRDVVSGMP
                     LDPDEEYCYEVERGPVKLDESECPRGLGLSGRTLLDMDQGGYSGAATRQQSKALMRYI
                     LNHYLGDQPLHTRQLLKDLQQL"
BASE COUNT         1601 a         1882 c         1905 g         1424 t
ORIGIN      
        1 gagatcccaa caagttaatg gtcggggaat gggttgtggc gatcggctcg cctttcggct
       61 ttgagaacag cgtaaccgcg gggattgtta gcgcgaaggg acgctcgctc gcgcaggaga
      121 actttgttcc attcatccag accgacgtgg cgatcaaccc tggcaattcc ggcggtccat
      181 tatttaacct gaaaggggaa gtggtaggaa tcaattcaca aatctacagc cgcacgggcg
      241 gcttcatggg actgtcgttc gccattccca tcgatgttgc catggatatc gtgaatcagt
      301 tgattgccac cggaaaggtc agtcggggac ggatcggcgt gatgattcag gaagtgacca
      361 aggaactggc ggaatctttc ggcctgtcca aaccggccgg tgcgctggtt gcttcagtgc
      421 aaaagggagg acccgccgaa aaagccggta tcgaaccgcg ggatgtcatc ctgaaattcg
      481 atggaaaacc tgtcaatgct tcaggcgacc tgccacgtat cgtagctgcg acgaaacccg
      541 gaagcaaggt tcaggtgcaa gtctggagaa acggcgcaac caaggacatc acagtcaccg
      601 tcgatgaact gccgccggac gagaagcctg gttcccgtgg gggcaagcgc ggcaaggcga
      661 cgcctgatac ctccaatcgg atcgggctca tgttgagcga gctcagccct gaccagaaaa
      721 aacagcttga ggtggaaaac gggctgctgg tggaagacat ggtgcccggc gttggcagcc
      781 gtgccggcct tcgccctggt gatgtgattc tcagcatcaa caaccaggat gtaagcagcg
      841 tcgaccagtt caaccagttg ctcaacaaga taccgaaggg ccgcaatatc gcgcttctta
      901 tcaggcgcgg cgacgccacc acgtttatca ccatgaaagt gaatggcgac aacaagtaat
      961 ccgtcgcccc ccctttccag cgcaaaccct cccgagttgg tcgtgtacag ccgggaggga
     1021 tgccatctct gcgaggatat gatctccgcg ctgcagcaat ggcaagagcg ggaacgcttc
     1081 agttttgaaa tagtcgacgt ggacagcgac caaaacttga ggctgcgcta cggagagcgc
     1141 gtccccgtgc tgattgccgg agagacggag gtatgccatt atcatctcga tcctctcgaa
     1201 tttggcaagc tcttctcttc cccgtcatga cgagctggga ttacggcagc cgtgccgtcg
     1261 cgcatgtcgc ggagtaatgc tcaagttcct gatgcttgtt tcgccaaaat tcgctaaaat
     1321 tcctgcttcc tgaagaggac gccggtctcg ggcgcaccgg ttctccgcgc cgccggtaat
     1381 cgggagggca ccgcaagtgc cctttttgtt atggcgacaa cagtatcggc cgcccgatgg
     1441 cagtaaatcc gattacctac cccaatcgca aatccctgat gctgcacatt cgcaacttct
     1501 ccatcattgc ccacattgat catggcaagt cgacgcttgc ggaccggata atccagatgt
     1561 gcggcgggct gtccgaccgg gagatggaag cccaggtcct ggattcgatg gatctggagc
     1621 gcgagcgtgg cattacaatc aaggcccaga cagcagcgct cgaatacaga tcgcgcgatg
     1681 gcaatcaata cctgttaaac ctgatcgaca cacccggaca cgtggatttt tcgtatgaag
     1741 tatcccgttc cctggcagcc tgcgaaggcg ccctcctggt cgtggatgct tcccaggggg
     1801 tggaggcgca gactgtcgcg aattgctata ccgcgaccga gcagggcgtg gaagtcgtgc
     1861 cggtgctgaa caagatcgat ctgcccgctg ccgatccagc gcgcgtgatc aaggaaatcg
     1921 aagatatcat cggcattgac gctcaagatg cgctttatgc cagcgcgaag actggccagg
     1981 gcgtggacga aatcctggaa gccgtcattt cgcgcattcc accgcccaag ggtaaccccg
     2041 cagcgccgct caaggccttg atcatcgact catggttcga taactatgta ggcgtcgtga
     2101 tgctggtacg ggttttcgac ggggtgctca aacccaagga caagatcctg ctgatggcca
     2161 gcaaggctgt gtacctgtgt gaacaagtcg gcgtttttac gccaaaatca agaaataggg
     2221 aatcgctgag cgccggcgag gtaggattca ttatctccgg gatcaaggag ctgaaatccg
     2281 ccaaggtcgg cgacacgata acattagccg accgtcccgc cgagcaacct ctgctgggct
     2341 tcaaggaaat aaagccccag gtgtttgccg ggctttaccc tgttgaatcc aaccagtatg
     2401 acgcgttgcg ggatgcgctg gaaaagctga agctcaatga ttcttccctg cactacgaac
     2461 ccgagacttc gcaagccctt ggtttcggct tccgctgcgg gttcctgggg ctgttgcatc
     2521 tcgatatcgt ccaggagcgg ctggaacgcg aatatggaat ggatctcatc acgaccgcgc
     2581 ccacggtgat ctatcagatc gtcctgcgcg acggctcggt catcgaaatt gaaaatccct
     2641 cgcggatgcc tgatccatcc aaaatccagg aaatccgcga acccatcatt actgccacca
     2701 ttcttgttcc acatgagtac gtgggatcag tcatcacgct ttgcatcagc aagcgcgggg
     2761 cccagaaaaa catgcagtac atgggcaggc aggtgatgct cacgtatgaa atgcccctca
     2821 acgaagttgt aatggacttt tttgatcgat tgaaatccgt gagccgcggc tatgcttcgc
     2881 tggattacga attcaaggaa ttccgtgctt ctgatctggt caagctcgat atactcatca
     2941 atggcgaaaa agtcgacgcg ctgtcactta tcgtgcatcg cgccagcagc cagtaccgcg
     3001 ggcgggagct tgcagaaagg atgcgcgagc tgattccccg gcagatgttc gatattgctg
     3061 ttcaggccgc aatcggctcg cacattatcg caagggaaag catcaaggcg ctgcgcaaga
     3121 acgtgcttgc caagtgctat ggcggcgata tcacgcgaaa acgcaagctg ctcgaaaaac
     3181 agaaagcggg gaaaaagcgc atgaagcagg ttggcaatgt cgagattccg caggaagcgt
     3241 ttctcgccat tctgaaagtg ggcgaaaaat agatgttatc tgaaccgatg acggaagtgc
     3301 acagggcgcg ggaccactcg cccgctctat tgaaagccgg gccgagaggt agggcatgaa
     3361 ttttccgctc atcatgttca tcctgctgct ggtgaccgga ggcatcgtgc tgctggaccg
     3421 gtttgtcctg caacctcggc gcggtgctgg aaccgaggga ccctggtggg tggagtatcc
     3481 gagaagtttt ttcccggtca ttctcatcgt tttttgcctg cgctcattcc tggtggagcc
     3541 gttcaagatt ccttcaggat cgatgatccc gaccttgctt gtcggggatt tcatcctcgt
     3601 gaacaaatac acctatggaa tccggctacc ggtcatcaac atgaagatac tggatatgaa
     3661 ggagccgcag cgcggagaag tcatggtatt ccgttatccc gagaacccgt ccatggacta
     3721 tatcaagcgc gtcatcggca tcccgggtga ttccgtgagc taccgtgaca agcagttgtt
     3781 catcaacaac agcccggtaa aaatggagcc ggacggggaa tacacctatg tggaatccgg
     3841 gttggcctat gtttatacca agcgcttcaa tgaatccttt ggtgggcccg accacaagat
     3901 tttgatcaat ccggagcttc ccgatcttat gctcgcgggc gtgcgccgct tccccctcca
     3961 ggaaaactgc acgtacgatg acacggggtt tacctgcaaa gtccccgcgg gaaactattt
     4021 cacaatggga gacaaccgcg acagcagcag cgatagccgc tattggggtt ttgtgccgga
     4081 aaagaacatc gtgggcaagg cattcctgat ctggtggaac ttcagcgacc tcaagcgagt
     4141 cgggacgtca atcgaataat tgaaataaga accgcaaata aattcctgcg gttcggataa
     4201 tcatgcggga aattgccaaa accctgctcc ggcatctgct atttttcggg aaatgaacaa
     4261 tgatacgctc tgccgcaaga tcggctacgc tttcaggcaa cccgagtttc ttcgccaggc
     4321 cttgactcac cgcagccata gcctgcccca caacgaaaga ctcgagtttc tgggggacag
     4381 cgtgctcaac tgcgcggtgg ccacgctgct tttcaggcat tttcccgatc gcgccgaggg
     4441 cgatttgtcg cgccttcgcg ccaacctcgt gaaccagcag gcgctcgcgg acattgccca
     4501 gatgctcgat ctcggcaagc tggtgagatt tggcgaggga gaaataaaaa gcggcggcaa
     4561 tcgccgtcct tcgattcttg ccgacgcgct cgaggctgtt ctcggcgcga tctatcttga
     4621 tggtggtttt gcagcggctg aaaaagtcat tacaacgctt tatacccctc tgttgagcga
     4681 gctgaatacc gatacgccgg gtaaagaccc caaatcgcta ctgcaggaat atctgcagag
     4741 tcgtcatctg gctccccctc gttatgctgt gattgccaca acaggcgaag cccaccagca
     4801 gcaattcgag gtggaatgcg tcatatcgaa gctcaacatg agaacgctgg gtgaaggggc
     4861 ctcgcgccgg agcgcggaac aggaagctgc aagggctgcc tatagacaac tccattcaca
     4921 aacctgaaag ctcaagcagg cacaccaatg catgatatcc accgcctctg tcgctcgtcc
     4981 ccccgcccgt ccattgaaga agtgggaaca ggattcggcc acgcgctacc ctgcctccga
     5041 tgaccaacgc tggcgatcac attaccgatt caggctatcg atgcggactg atcgccatag
     5101 tgggacgtcc taacgtcggc aaatcgacgc tgctcaaccg gctggtcggg cagaagataa
     5161 gcataacgtc aagaaagtcg caaaccacgc gccatcggat cctgggtatt gtcacccggc
     5221 ctgagtcaca gtttatcttt gttgacaccc ccggttttca gtcgcaacac ggaaatcgtc
     5281 tgaatgcgat catgaaccgg accgttcagc agagcgtcca tgaggttgat gtcgtagtgt
     5341 tcgtcatcga ggccctgcat ttcgatgagc gcgacaggcg ggtgatgaaa cttctgcccg
     5401 cgggaaagcc ggtgatcctg gctatcaata aaattgaccg gctggcggac aaggcacctc
     5461 tgctcccttt cattcaaaaa atggccctgg agctcaatcc gacaacggtg gttcctgtaa
     5521 gcgcagagcg gggaacgcaa ttggacaatt tgctctccgc aatcaggccg tatctccccg
     5581 aacaacccgc attgtttggc aatgacgaag tcaccgaccg cagcgagcga ttcattgcgg
     5641 ctgaactggt tcgggaaaag cttttccgcc ttctcggcga agagcttcct tattcaacca
     5701 ccgtcatcat cgatcagttc agcaccgaag gcgaactgcg caagatccac gcctctgtaa
     5761 tcgtcgacaa gccaggccag aaggccatcg tcatcggtca aaatggcgaa aagctcaagc
     5821 tgatcggaac ctcggcgcgc aaggatatgg aagaagtctt cgggggcaag gtctatctcg
     5881 agatatgggt taaagtgaaa agcggctggg cggacgatgt tcacgctttg aaatccttcg
     5941 gctatgagta gcattgcccg ccgtgcatag cttcgtcctg agattccagg attgacggca
     6001 ggctgatgag cgttgacaag caggacgcgc agccagcatt catactgcat tcctacccct
     6061 atagcgaaac cagcctgatc gtggaagcct tcacccgata ttccggacga ctgcccttgc
     6121 tggcaaaggg cgcaaaacgt ccccggtccg cgctcagggg aatgctgaga gcatttcagc
     6181 cattgcagct gagctggggg gggaaatcag agctgcgcgt attgcacaag gcggaatggc
     6241 aaggcggttt tataccgctg cagggcaccg gattgatctg cggcttttac ctcaacgagt
     6301 tgctcatgcg cctgctgcac cgcaatgacc cgcacgaaca actgtttgta cactaccagg
     6361 atgcgctggc cgcgttgagc gagcatagcg acaccatctc gatactccgc aaattcgaac
     6421 agcggttgtt acgggaactc ggttacgccg tgacgctcga ccgcgacgtt gtgagcggca
     6481 tgcccctcga cccggacgaa gagtattgtt acgaggtcga gcgcggcccg gtcaaactgg
     6541 acgaaagcga gtgtccacgc ggccttggat tgagcggccg aaccctcctc gatatggacc
     6601 agggcggtta ttccggcgcc gccacaaggc aacagagcaa ggccctcatg cgttacattc
     6661 tcaaccatta tctcggcgac cagccgttgc acacccgcca gttgttgaaa gatttgcagc
     6721 aactttaacc catggcaaaa gaaatcgtga gaatccctga agtaactggg caccctttgt
     6781 gaaagatgat tgcgtcctca cggttaacca tc
//
LOCUS       DASSHJ010000165        15650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1199163,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000165 DASSHJ010000000
VERSION     DASSHJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 15650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15650
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199163"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /gene="glgC"
                     /locus_tag="VFI43_06340"
     CDS             complement(<1..1013)
                     /gene="glgC"
                     /locus_tag="VFI43_06340"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET7061781.1"
                     /translation="MPQGADARFHSYITRNSIAFILAGGRGSRLRQLTDWRAKPAVPF
                     GGKFRIIDFALSNCVNSGIRRIGVATQYKAQSLISHIQHGWGFLDGRFKEFVELLPAQ
                     QRTEESWYRGTADAVFQNIDILRSHKPEYVLILGGDHIYKMDYSKLLADHVDKGADMT
                     IACVDVPLESAGELGIVSVDGNWRINDFQEKPRHPTPIPGQPSQALASMGVYVFNASF
                     LYDQLIRDHDEADSSHDFGKDLIPYSVPRHKVFAHRFIDSCVNMASGVPYWRDVGTID
                     AYWAANLDLTHVTPDLNLYDQDWPIWTHQEQLPPAKFVFDDEDRRGQALDSMVSGGCI
                     ISGS"
     gene            1166..3376
                     /gene="glgB"
                     /locus_tag="VFI43_06345"
     CDS             1166..3376
                     /gene="glgB"
                     /locus_tag="VFI43_06345"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET7061782.1"
                     /translation="MQKRIARHDMPGNMDPALAALLSGRLHNPFSYLGFHAEQDHSLV
                     RVFYPYASHVWINIAGKFEPMQRTHPSGIFEWKGKTRPSLPYLLRVQDHISVPKDTAF
                     ETYDAYAFPPRISEYDLYLFHEGRLWQAYRTLGSHVIEIDGVAGVRFAVWAPNAARVS
                     VVGSFNRWDGRVHTMAVHESSGVWELFIPGVPQDSLYKYEMLSHETGEILLKTDPYAE
                     RYELRPRNASLTPCPGKAVWCDDTWMEARATSDWLHTALNIYEMHAGSWKRNLDGSFL
                     SFRELAIELIPYVLDMGFTHVELLPITEHPLDESWGYQTTGYFAATSRHGTAEDLKFF
                     VDACHEAGIGVILDWVPAHFPNDAFALAHFDGTALYEHADRRMGFHEDWGTHIFNFGR
                     NEVKSFLLSSAHYWLSEFHLDGLRVDAVASMLYLDYSRKPGEWLPNKYGGRENLEAID
                     FLRELNIMVHQEFPGALTVAEESTAWPGVSRPTYLGGLGFSMKWNMGWMNDTLAYMHH
                     DPVYRRFHHDKLTFGQLYAYSENFVLPLSHDEVVHGKGSLLEKMPGDNWQRRANLRLL
                     FCFQMTWPGKKLNFMGNEFGQRTEWNAGKGLDWEQLNQPEYQGILNAFRDLGRLYRNL
                     PALHQLDFSSGGFVWIDCHDADNSVISYLRQAKDGVFVVVVLNFTPVPRHHYRVGVPK
                     GGTYNEIFNSDASLYGGSGVGNMGHARATPKPWNGQPHSMSITLPPLGGLIFSL"
     gene            3458..4963
                     /gene="glgA"
                     /locus_tag="VFI43_06350"
     CDS             3458..4963
                     /gene="glgA"
                     /locus_tag="VFI43_06350"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET7061783.1"
                     /translation="MVAAGSHDSRLRVLFVTPEIHPLNKTGGLGDVSAALPAALRELH
                     VDARVLIPGYPQVMSGLRHKRKIAEFDAQGPLLPCALFSAELPVSRSEELPLLVIDCP
                     PYYQRDGGPYSDASGRSWPDNDVRFGLLSKIGAILASDSTPIDWHPHVAHCNDWQAGL
                     LPAYLHFHPGKRAATLMTIHNLAFQGVFPPDTVTKLGLPASSFGVNGVEYYGGMAFLK
                     AGLYYSDHISTVSPTYAREIQAAPLGFGMEGLLASRHNEISGILNGIDTDEWDPGNDP
                     YLARHYSISNLAAKSVNKKELQQVLGLDVDPRFPLFGAVSRITYQKGCDLLLRIAKEL
                     IAIPAQLAVLGNGEAVLEREFSMLAKSNPGKIAVSIGFDEKLAHMIEAGADSFLMPSR
                     FEPCGLNQMYSQRYGTPPLVHATGGLIDTVADFTPASLAAGKATGFLFQDMTPESFLA
                     GIRRVVDAYNDKPDWQRLKINGMTKDFSWRSSATAYREIYSRLSSLSSLPL"
     gene            complement(4974..7526)
                     /gene="glgP"
                     /locus_tag="VFI43_06355"
     CDS             complement(4974..7526)
                     /gene="glgP"
                     /locus_tag="VFI43_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET7061784.1"
                     /translation="MTIGTAFTLTVNPKLPPNLSRLEELANDLWYSWDRSTRSLFSRL
                     NPTLWGAVGHNPKAFLKRVDESLLLKAAEDQVFLANYHSILSAYDSYRSAPARREGAA
                     NLKPSDRVAYFCFEFGFHESFPIYSGGLGILAGDHCKAASDLRLPLVAVGLLYRQGYF
                     FQTIDSQGNQRVTYTDSDFEDMPVSPMLHSDDSEIRINVELPHRSVQAKIWCAKIGHV
                     TLYLLDTDLPENSVDDRDITHQLYGGDRVMRIEQEILLGVGGVRALQAIGIKPTVWHI
                     NEGHAAFMMLERMRGLVREGLNFPAALEAVAANTVFTTHTAVPAGHDHFSDDMVTAYF
                     EQLYHGLGISREEFLALGHVPDNPDFNMTALAVNISRYQNGVSKIHGNVSARICKDLW
                     PQIEPQENPMGYITNGVHVPTFLAQEWTDLFDRYLGYEWRNNICDTQYWSRISAIPDH
                     LFWSVRQTLKSQLFHGMRARIATQNTRIRGSEAHLDRLLKLIDPVNPSILTLGFARRF
                     ATYKRAGLLFEDLEWLRRLVLDQERPLLIVFAGKAHPADVPGQDLIRRVSQIARMPEF
                     EGRIFLLEDYDLRLARRLTAGVDVWLNSPVYPLEASGTSGMKAGINGTLNLSVLDGWW
                     GEGYDGKNGWAIKPGPENMDPARRDKEESKDFYETLQDHVVPLYYTRDKPGYSSAWVK
                     MAKSSMMSLLPRYNAARMVNEYTHKFYVPASRQGKLYAANDFEAAKKIADWKAFVRQA
                     WGGVRIWRLDTPSKRISFGDGLQFKAGVILNGLRAEDIVVELLISRQIKKSHLREFKH
                     YKFEPVGEESGERIYQLDLTPELCGKLEYFIRAYPYHPLLTHRFEMGMMAWV"
     gene            complement(7591..8136)
                     /gene="orn"
                     /locus_tag="VFI43_06360"
     CDS             complement(7591..8136)
                     /gene="orn"
                     /locus_tag="VFI43_06360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET7061785.1"
                     /translation="MTQDSQNLIWIDMEMSGLSPENDRIIEVALVVTDSQLNVVAEAP
                     VLVVFQPDTVLDAMDSWNRSTHGKSGLIDKVRSSQLAEAEVEASMIVFLQTYVPPGVS
                     PMCGNSICQDRRFLGRWMPRLESYFHYRNLDVSTLKELAKRWRPQVLAGLAKQSRHEA
                     LADIYDSIAEMKHYREHFIRM"
     gene            8245..9492
                     /locus_tag="VFI43_06365"
     CDS             8245..9492
                     /locus_tag="VFI43_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7061786.1"
                     /translation="MQIFTTVFLGALLLTLAARIWLSARHIQHVLAHRDRVPDNFASE
                     ITLESHQKAADYTCAKTRLGYVDMVLGTVLVLLLTLGGGLNALSDFWGVWLKDPLFHG
                     MALIISAVLLISLFELPLSYYRVFVIEQQFGFNRMTPRMFVTDLVKRTVLAILIGAPL
                     VLGILWLMEKAGSNWWFYAWLAWMAFNLLVLAVFPTWIAPLFNKFTPLNDAALRDRIE
                     NLMRKCGFKTSGLFVMDGSRRSNHGNAYFTGFGTSKRIVFFDTLLARLNASEIEAVLA
                     HELGHFKRRHVIKRIAWTFAGSLFFLWALGYLMQQGWFYEGLGVHEHSPAVALLLFFL
                     VMPAFTFLLQPLASLYSRKHEFEADEYAAHNASALDLIRALVKLYQDNAATLTPDPLH
                     SAFYDSHPPATLRIARLQRLAHN"
     gene            9537..9878
                     /locus_tag="VFI43_06370"
     CDS             9537..9878
                     /locus_tag="VFI43_06370"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7061787.1"
                     /translation="MENGTHELTTKKCRPCEGGTPPLSPKEVSELMRQLDGWEFLQTR
                     IAKDYHFKNYYQTIAFVNAIAWISHREDHHPDLTVEYNKCRVEYMTHAIGGLSENDFI
                     CAAKIDALLKI"
     gene            9875..10801
                     /gene="rsgA"
                     /locus_tag="VFI43_06375"
     CDS             9875..10801
                     /gene="rsgA"
                     /locus_tag="VFI43_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7061788.1"
                     /translation="MRPHLLPGVTPLVEGQIIASFGRQFLVKTGEGDTLSCVARGKKG
                     GAACGDRVQIRTVEPGRQGVIETILPRTALLHRSDAYREKLIAANITQVVIVVAAVPS
                     FSEELINRFLAAAENQRLKLLIVLNKADLVEATRAAAEALKLYQELGYTLLKLSASTD
                     PMPLRPYLSDQLSVFVGQSGMGKSTLINALLPGANRATASISVALDSGRHTTTHARLY
                     EIDGQSAIIDSPGLQEFGLYHMNNEELAWGFLEFRPYLGRCKFADCRHLQEPSCALVD
                     ATAQGGIAKRRLHFYQMLATRLTSRSGGSSPD"
     gene            10823..11092
                     /locus_tag="VFI43_06380"
     CDS             10823..11092
                     /locus_tag="VFI43_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061789.1"
                     /translation="MKAGILEIDYLYPCHSTALNFGLKLHLPQPDRPLYLETVQREIS
                     KQHCFSFGTAVRTEIQNGWGREGAFAPSADELMQMGSDQIFHLFK"
     gene            11245..11586
                     /locus_tag="VFI43_06385"
     CDS             11245..11586
                     /locus_tag="VFI43_06385"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7061790.1"
                     /translation="MHTCSHAVVGCMDFRIQKTVSNLLEHLKVHEGTFDRVTFAGGAA
                     NKEQLKHHLELSKRLHDSGMAILSVHEDCGAGAKKEDLYEAAETARSLGYDPKMFYIK
                     LDGTWEEVKPA"
     gene            complement(11696..12187)
                     /locus_tag="VFI43_06390"
     CDS             complement(11696..12187)
                     /locus_tag="VFI43_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7061791.1"
                     /translation="MARPATGREFVETAKERIASAKTVDALRAAQALLLPLEFGLSLE
                     QTAMIIGLSKSRTGKLRTRFQRIETGVEQVKTKKGLRNHARMSLDEEVKFLAPFVSEA
                     KDTGVLHIPQLKAELERRVGRQISTSTVYQLLRRHGLSKLAQHPRTDMTVAQAWERLA
                     QKK"
     gene            complement(12393..12716)
                     /locus_tag="VFI43_06395"
     CDS             complement(12393..12716)
                     /locus_tag="VFI43_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7061792.1"
                     /translation="MRTDALIAMKRIEIVIEEESLGDLIGLFKSVNVRGYTFIKHAGG
                     LGTRGERHPDDYALQEKNAVVILACEENQAERVINAIRPRLKDFGGMCLVSDCQWVIG
                     PAVSY"
     gene            complement(12838..13821)
                     /locus_tag="VFI43_06400"
     CDS             complement(12838..13821)
                     /locus_tag="VFI43_06400"
                     /inference="COORDINATES: protein motif:HMM:NF017765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HET7061793.1"
                     /translation="MSSLLVPAILFFALGMFACLIKSDLKFPPDMHKMIVIYLLIGIG
                     LHGGRAFINSPIGDAMPAVAAAFFFGIFLPILAYIILRGVGKIDPLNAAAISAHYGSV
                     SAGTYMTAVAMLGSMGVTYEAYPVIMLAIMESPAIMIGLVLAGYSRKIMGGAQKGEKG
                     MYGHLVREAFTNGSILLLFLSMGIGYVVSDASYKKIEPFFEGVFMGALCIFLCDMGME
                     AGKRISEFKTVGVFLVAFGVVMPIIGATFGMLVGHYFLQYSVGGVTLVTVLAASCSYI
                     AVPPAMRLAIPEANPSFYLTLSLGVTFPFNIVIGVPTYYAIAQYLAGQGAA"
     gene            15530..15646
                     /locus_tag="VFI43_06405"
     CDS             15530..15646
                     /locus_tag="VFI43_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061794.1"
                     /translation="MIALQQTNLRRLLAYASVAHRAGNRRFQARADFRQNCS"
BASE COUNT         3594 a         4247 c         4090 g         3719 t
ORIGIN      
        1 gacccgctta tgatgcagcc gcctgaaacc atcgaatcca gtgcctgacc gcgacgatcc
       61 tcgtcatcaa aaacgaattt ggccggaggc aactgttcct gatgcgtcca tataggccag
      121 tcctggtcgt atagattgag atccggagtg acgtgggtaa ggtcaagatt cgccgcccag
      181 tatgcatcga tcgtgcctac gtcgcgccag taaggaaccc ccgaggccat gttgacgcaa
      241 ctgtcgatga agcgatgggc aaacaccttg tgacgcggca cagaatacgg aatcaggtct
      301 ttgccgaaat cgtgggacga gtccgcttcg tcatggtcac gaataagctg gtcataaaga
      361 aacgaggcat tgaatacata gacgcccatg ctggcaagcg cttgggacgg ttgaccggga
      421 ataggggtcg ggtgcctggg cttttcctga aagtcgttga tccgccagtt gccgtccacg
      481 cttacaatgc ccaactcgcc tgccgattcc aatggcacgt cgacgcacgc aatagtcata
      541 tccgcgcctt tatccacgtg atctgcgagc aacttgctgt aatccatttt atagatgtga
      601 tcgccgccca ggatcaacac gtattcgggc ttgtggctgc gcaggatatc aatattctgg
      661 aacaccgcgt cggctgttcc gcgataccag ctttcctcgg tgcgttgctg ggcaggaagc
      721 agctcgacga actccttgaa ccgcccatca agaaaacccc atccatgctg gatatgactg
      781 atgaggcttt gcgccttgta ttgggtcgca acgccaatcc ggcggattcc tgaattcacg
      841 cagttggaaa gtgcgaaatc gataatgcgg aacttccctc caaacggaac ggcgggtttg
      901 gcacgccagt ctgtgagttg gcgcaatcga ctccctcgcc ccccggccag gatgaaggcg
      961 atcgaattac gcgttatgta gctatgaaaa cgggcatccg ctccttgggg catggcagcg
     1021 attcctgaaa aatgtcattg aaggcgaaca agctgcttgg cctgcccttc cagatagcga
     1081 atatactcac tcaccccctg attcatgggg tcgaacaagc aggacaatac catccttccg
     1141 cgcacatgcg gctataattc gccacatgca aaagagaatt gcaagacatg acatgccagg
     1201 taacatggat cccgcgcttg cggcgctgct ttcgggacgg ctgcacaatc cgttcagcta
     1261 ccttggcttc cacgcagagc aagatcacag cctggtgagg gtattttacc cctatgccag
     1321 ccatgtatgg atcaatatag caggcaaatt cgagcccatg cagcgcaccc atccctctgg
     1381 aatttttgaa tggaaaggca agacaagacc ttccctacct tatctgctgc gtgtccagga
     1441 tcatatcagc gtgccgaagg acaccgcatt tgaaacctat gatgcttacg ccttcccccc
     1501 acgcatttcc gaatatgact tgtacctgtt tcatgagggg cgcttatggc aggcttatcg
     1561 cacgctcggt tcccatgtta tcgagattga cggagtagcg ggcgtccgtt tcgccgtttg
     1621 ggcgccgaat gctgcccgag tgagcgttgt cggcagcttc aatcggtggg atgggcgagt
     1681 gcatacgatg gctgttcatg aatcgagtgg agtatgggag cttttcatcc cgggcgttcc
     1741 ccaagactct ctctataaat acgagatgct tagccacgag actggagaaa tccttctcaa
     1801 aaccgatccc tatgccgaac gttatgagtt acgccccagg aacgcatcgc ttactccgtg
     1861 tccgggaaaa gcggtctggt gcgacgacac gtggatggaa gcgagggcga cctccgactg
     1921 gctccatacg gcactcaaca tttacgagat gcacgcaggg tcctggaagc ggaacctgga
     1981 cggaagcttt ttaagcttcc gcgaactcgc catcgaactg attccctatg ttcttgacat
     2041 gggcttcacc catgttgagt tgctccccat taccgaacac ccactggacg aatcgtgggg
     2101 ttaccagaca accgggtatt tcgccgcgac gagccgtcat ggaactgcgg aggatctcaa
     2161 gttttttgtc gacgcctgcc atgaggcggg tattggagtg attctcgact gggtgcccgc
     2221 gcattttccc aacgatgcgt ttgcgctggc gcattttgac ggaaccgcgc tgtatgagca
     2281 tgcggaccgg cggatgggat ttcatgagga ctggggtacg catatcttca acttcggacg
     2341 taatgaagtg aagtcttttc tgctatcgag cgcccactat tggttgtcgg aatttcatct
     2401 cgacggtctg agagtcgatg cggtggcatc catgctctat ctcgactatt cccgaaaacc
     2461 gggcgaatgg ctgcccaata aatacggagg gcgggaaaat cttgaagcca tcgatttcct
     2521 gcgtgaactg aacatcatgg ttcaccagga gttccccgga gcgctgaccg ttgcagaaga
     2581 gtctaccgcc tggccgggcg tttcacggcc gacatacctg ggcggcctcg ggttctccat
     2641 gaagtggaac atgggctgga tgaacgacac gcttgcatat atgcatcatg atcccgtcta
     2701 caggcgattt caccacgaca aacttacctt cggccaactc tatgcctaca gcgaaaactt
     2761 cgtccttccg ctgtcgcacg acgaagttgt gcatggcaaa gggtcgctcc tggaaaaaat
     2821 gccgggggac aactggcagc gacgcgcaaa tctccggctg ctgttttgtt tccagatgac
     2881 gtggcctggc aagaagctga atttcatggg gaacgagttc gggcagcgga cagaatggaa
     2941 cgccggcaag gggctggact gggaacagtt gaatcagcct gaatatcagg gcatcctgaa
     3001 tgcatttcgc gatctcggtc gcctttacag aaaccttccc gcgctgcatc agctggattt
     3061 ttcatcggga ggcttcgtat ggattgattg ccacgatgca gacaattcgg taatcagcta
     3121 tctccgccag gcaaaggacg gcgttttcgt cgtagttgtt ctcaatttca cgcccgttcc
     3181 acgccaccat tatcgcgtcg gcgttccgaa gggtggaacc tacaatgaga tattcaacag
     3241 tgacgcttca ctttatgggg ggagtggcgt aggcaacatg ggacacgctc gtgcaactcc
     3301 gaagccctgg aacgggcagc ctcattcgat gagcattacc cttccaccat taggcggcct
     3361 gattttttcg ctgtgaagtc caggtgctgt tgcaacgtta ataaatgagg tataggtgtt
     3421 cgtagagaag cccgggaatg atagcaggca aacccctttg gtcgccgctg gttcacatga
     3481 ttcgagactc cgggttcttt ttgttacgcc ggaaatacac cccctcaaca agacaggcgg
     3541 acttggagac gtaagcgcag cactacctgc cgcattgcga gaacttcacg tcgatgcccg
     3601 cgtccttatt cccggttatc cgcaggtgat gtccgggctc aggcataagc gcaaaatagc
     3661 ggagtttgat gcccagggcc ctttgctccc atgcgcacta ttttcagcgg aactgccggt
     3721 gagtcgatcg gaagaacttc ccttgctggt cattgattgc ccgccttatt atcagcgaga
     3781 tggcggccct tattccgatg catccgggcg cagttggccg gataacgacg tgcgcttcgg
     3841 gctcctttcc aaaatcggcg ccattctggc aagcgattca actccgatcg actggcatcc
     3901 ccacgtagcg cactgtaatg attggcaagc gggcctgctg cccgcatacc tgcattttca
     3961 tccgggaaaa agagccgcta cgctgatgac aatacataat cttgctttcc agggcgtctt
     4021 ccctcccgac accgtgacta aactgggact gcctgcctcg agttttgggg taaatggagt
     4081 ggaatattac ggagggatgg ctttcctgaa ggccgggctc tattattccg atcatatttc
     4141 caccgtaagc cccacctatg cgcgggaaat tcaggccgcg ccgctgggct ttggaatgga
     4201 aggattgctg gcttcccggc ataacgagat cagcggcatc cttaatggta ttgacactga
     4261 cgaatgggat ccgggcaacg atccgtacct tgcccggcat tattcaattt ccaatctcgc
     4321 tgccaaatca gtcaataaaa aagaactgca gcaagtattg ggcctggatg tcgacccccg
     4381 ctttccacta ttcggagcgg tgagccggat tacctaccag aaaggctgcg acctgcttct
     4441 gcggattgcg aaagagttga ttgcgatacc cgcgcaacta gctgtcctgg gcaacgggga
     4501 agccgtgctc gagcgcgaat tctcgatgct ggcaaaaagc aatccgggaa agattgccgt
     4561 gtctatcggt tttgatgaga agctcgcgca catgatcgaa gctggggctg acagtttcct
     4621 gatgccttcc cgcttcgagc cctgcgggct gaaccagatg tatagccaac ggtatggcac
     4681 gccacctctt gtgcacgcga cggggggcct gatcgatacg gttgctgatt ttactcctgc
     4741 gtctctggct gctggaaaag ccaccggctt cctgttccag gacatgacac ccgaaagctt
     4801 ccttgctgga atacggcggg tcgtagacgc ctacaatgac aagccggact ggcagcgtct
     4861 aaagataaat ggcatgacaa aagatttcag ctggcgttcg agcgccactg cttaccgcga
     4921 aatctactcg cgtctctcgt ccctgtcttc tctacctctt tgacgtcgcg tgttcagacc
     4981 cacgccatca tccccatttc gaaccgatgc gtcagtaatg gatgatacgg gtaagcgcgt
     5041 atgaaatact cgagcttccc gcacaactcc ggagtgagat ccaactgata aattcgctcg
     5101 ccggactcct cgccgacagg ctcgaattta tagtgcttga attcgcgcag gtgggatttc
     5161 ttgatttgac gggagatgag caactccacc acgatatcct cggctcgcaa gccgttcagg
     5221 atcaccccgg ctttgaactg caagccgtca ccgaagctta ttcgctttga cggcgtgtcg
     5281 agcctccaga tcctgacgcc cccccatgct tgtctcacaa aagctttcca gtccgcgatt
     5341 ttcttcgccg cctcgaaatc gttcgcggca tacagcttgc cttgcctgct cgcaggcacg
     5401 taaaacttgt gagtgtattc gttgaccatc ctcgccgcgt tatagcgcgg caggagcgac
     5461 atcattgaag atttggccat ttttacccag gcggaggaat agcccggctt atcccgcgtg
     5521 taataaagag gcaccacatg atcctgcaag gtttcataaa aatctttact ttcctccttg
     5581 tcccggcgcg ccggatccat gttctccggc cccggtttga tagcccaacc gtttttcccg
     5641 tcgtagccct ctccccacca gccatccagc acactcaggt taagcgttcc attaataccc
     5701 gctttcatgc cagaggtacc actggcctcg agtggatata cgggactgtt gagccatacg
     5761 tcgactccgg cggtcagccg acgcgccagc cgcagatcat agtcctccag aagaaatatt
     5821 cttccttcaa actccggcat gcgtgcgatc tggcttacgc gccgaatcag gtcctgcccc
     5881 ggaacgtccg cagggtgcgc ttttcccgcg aacacgatca gcagcggccg ctcctggtca
     5941 agcacaagcc ggcgcagcca ctcgaggtct tcgaataaca gccctgcgcg cttgtaggtc
     6001 gcaaaccggc gggcaaatcc aagtgtaaga atgctggggt taacggggtc gatcagtttg
     6061 agcaggcgat ccagatgggc ttcggaacca cggatgcgcg tattctgggt ggcgatgcgg
     6121 gcgcgcatcc cgtgaaacag ctgggacttg agtgtctggc ggacgctcca gaacagatga
     6181 tcgggaatgg cgctgatacg ggaccaatat tgcgtatcgc agatattgtt acgccactca
     6241 tagcccaggt acctgtcaaa taggtcggtc cactcctgcg cgagaaacgt gggtacgtgc
     6301 acaccattgg tgatataccc cattgggttt tcctgcggct cgatctgagg ccataaatcc
     6361 ttgcagattc gcgcggatac gtttccatgt atcttgctca ccccgttctg gtaacgtgaa
     6421 atgttgacgg cgagcgctgt catgttgaaa tcgggattgt cggggacgtg gcccaaagca
     6481 aggaattcct cgcggctaat ccccaggcca tggtaaagct gctcaaaata agcggtaacc
     6541 atgtcgtcgc tgaaatggtc gtgaccagcg ggtacggcag tgtgggtcgt gaacacggtg
     6601 tttgccgcga cggcttcaag cgctgcggga aagttcaacc cttcacgcac caatccgcgc
     6661 atccgctcga gcatcatgaa agccgcgtgc ccctcattga tatgccacac cgtcggcttg
     6721 attccaatcg cctgtagcgc tcttactcca cccaccccca agaggatttc ctgctcgatg
     6781 cgcatgaccc tgtctccgcc gtagagctgg tgagtgatgt cgcgatcatc cacggaattc
     6841 tccggaaggt ccgtatccag cagatacagc gtgacgtggc cgatttttgc gcaccagatc
     6901 tttgcctgga cactgcggtg cggcaattcg acatttatcc gaatctccga atcatcgcta
     6961 tgcaacatgg gcgataccgg catatcctca aaatctgaat ctgtatatgt gacccgctgg
     7021 tttccctggc tatcgatcgt ctggaaaaaa tatccctgtc gataaaggag tccgacggcg
     7081 accaaaggca ggcgcagatc gctcgccgcc ttgcaatgat ccccggcaag aatgccgaga
     7141 ccaccagagt agatgggaaa gctttcgtgg aacccgaatt caaagcaaaa ataggcgacc
     7201 cggtcacttg gcttgaggtt ggcggcgcct tcccgccggg caggcgcgga gcgataggaa
     7261 tcgtaggcgg agagtatgct atggtaattc gccaggaaaa cctgatcctc ggcagctttc
     7321 agaagcagcg attcgtccac ccgcttgaga aacgccttgg gattgtggcc gacggcgccc
     7381 caaagggtgg gattgaggcg cgagaatagt gaacgggtcg aacgatccca gctataccag
     7441 aggtcattgg ccagctcttc gagccgagaa aggtttggcg gcagttttgg attgacggtg
     7501 agcgtaaacg cagtgccgat cgtcatgcga tagttccttc aagcgtggaa aaatgtgatg
     7561 gaaaccacga gcgggtcaag cgacctggct ctacattcta atgaaatgct cgcggtaatg
     7621 cttcatctcg gcaatcgagt cataaatatc cgccagcgcc tcatgcctcg actgctttgc
     7681 cagacccgcc aggacctggg gcctccagcg ctttgccagt tctttcagcg tactgacatc
     7741 gagattgcgg tagtgaaaat aggactccag cctgggcatc catcgcccga ggaagcggcg
     7801 gtcctggcaa atggaatttc cacacatggg ggaaacaccg ggaggaacgt aagtctgaag
     7861 aaacacgatc atggaagctt cgacctcggc ctccgctaat tgcgacgatc tgactttatc
     7921 gatcaggccg gatttgccgt gggtggacct gttccagcta tccatggcat caagtaccgt
     7981 atccggctga aagacaacca gcactggtgc ttcggccaca acattcaatt gcgagtcggt
     8041 tacgaccagg gcaacctcga tgatacgatc gttctctgga ctgagaccgc tcatttccat
     8101 gtctatccag atgaggttct ggctgtcttg tgtcataatt ggatcgatcc ggatgaattt
     8161 tcaatgcgtc attgtgtcat atcagcacca ctccgtcatc cggcgattcc ggacactgac
     8221 gattcctaac actctttctt ttttatgcag attttcacga cggttttcct gggcgcgctg
     8281 ttattgactc ttgccgcccg catctggctt tccgcccggc acattcagca tgttcttgcg
     8341 caccgggaca gagtacctga caacttcgct tcggagatca cgctggagtc tcatcaaaag
     8401 gctgccgatt acacctgcgc caagacccgg ctgggatacg tcgacatggt gctcgggact
     8461 gtccttgtgc tgctattgac gctgggcgga ggcctgaatg cattgtcgga tttctggggg
     8521 gtgtggctga aggacccgct ttttcatggc atggccctca tcatcagcgc ggtactgctc
     8581 ataagtctgt ttgagcttcc cctgagctac taccgggtat ttgttatcga gcagcagttt
     8641 ggcttcaata gaatgactcc acgcatgttc gtaaccgact tggtaaagcg gactgttctt
     8701 gcaattctaa ttggcgcgcc gctcgtgctc ggtattttat ggctgatgga gaaagcaggt
     8761 tcgaactggt ggttttacgc ctggctcgcc tggatggcat tcaacctgct tgttctcgca
     8821 gttttcccga catggatagc gccgcttttc aacaaattta cgcccctcaa tgatgccgcc
     8881 ctgagagatc gcatcgagaa cctgatgcgt aagtgcggct tcaagacaag cgggctgttc
     8941 gtgatggatg gctcgcggcg aagcaatcat ggtaatgcct atttcacagg ttttggcacg
     9001 tcgaaacgca tcgttttttt cgacacgctg ctcgcgcgtc tgaacgcatc cgaaatcgaa
     9061 gccgttttag cgcacgagct gggccatttc aaaagacgtc atgtgatcaa gcgcatcgcc
     9121 tggacctttg ccgggagcct gttcttcctg tgggctctcg gctatcttat gcagcagggc
     9181 tggttttacg aggggcttgg ggttcacgaa cattcccccg cagtcgcctt gctgttgttc
     9241 tttctcgtta tgcctgcgtt tacgttcctg ctgcagccgc tcgccagcct gtattcgcga
     9301 aaacatgaat ttgaggcgga cgaatacgct gcccataatg cgtctgcgct cgatcttatc
     9361 cgagcccttg tcaagctcta ccaggacaat gccgccacgc ttacccccga tccgcttcac
     9421 tccgcatttt atgattccca tcccccggca acgctaagaa ttgcgcgcct gcaaagactg
     9481 gctcacaatt gaggccaggg acaggaatga attcacacta aaggaaagga gcgcagatgg
     9541 aaaacggtac tcacgaactc accacaaaaa aatgccgccc atgtgaaggg ggcacaccac
     9601 cactttcccc aaaggaagtg tcggagctga tgcgacagct cgacggatgg gaatttctcc
     9661 agaccaggat tgccaaggat tatcatttca agaactacta tcagaccatt gcattcgtga
     9721 atgccattgc ctggatttca catcgcgagg atcaccatcc cgaccttact gtggaataca
     9781 acaaatgccg ggtcgagtat atgactcatg ccatcggggg gttgtccgag aacgatttca
     9841 tctgcgcggc gaagatagac gcccttctca agatttgaga ccccatctcc ttcctggcgt
     9901 tactcccttg gtagaagggc aaatcattgc cagtttcgga aggcagtttc tcgttaaaac
     9961 aggagaaggc gatacgctct catgtgttgc gcggggtaaa aaagggggag cagcatgtgg
    10021 agatcgggta caaatccgta ctgttgaacc agggcgtcag ggagtcattg agacaatcct
    10081 gccacgaaca gcgctattgc atcgcagcga cgcctatcga gaaaagctga tcgcggcaaa
    10141 cattactcaa gtggtcattg ttgtcgcagc cgtgcccagt ttcagcgagg aactgatcaa
    10201 ccgtttcctg gctgctgcag aaaaccagcg actcaagctg ctcatcgttc tcaacaaggc
    10261 ggatcttgtg gaagcaacca gggcagcggc tgaagcactt aagctctacc aggaattggg
    10321 ctacacgctc ctgaagctca gcgcttcgac tgacccgatg cccttgcgac cctatctatc
    10381 ggatcagctg agcgtgtttg tcggacagtc agggatggga aaatcgacgc tgataaacgc
    10441 ccttcttcct ggtgcaaaca gggcaacggc cagcatttcg gtcgcgctgg atagcgggag
    10501 acatacgact acccacgcgc gactgtacga gatcgacgga caaagcgcta ttatcgactc
    10561 ccctggcttg caggagttcg gcctttacca tatgaataac gaggagctgg cgtggggctt
    10621 tctcgaattt cgtccatacc tcggccgatg caagttcgcc gactgccgcc atcttcagga
    10681 accaagttgc gcccttgttg atgcaacggc tcagggcggg atagccaagc gccgcctgca
    10741 tttctatcag atgctggcga cgaggctgac gtcgagatcg ggtggttcgt ccccggattg
    10801 aaacattcag ggacgaaccc gcttgaaagc aggaatattg gaaattgatt acctttatcc
    10861 ctgccactct accgccttga acttcggtct taaactccat ttaccgcagc cggatcggcc
    10921 tctctacctt gaaacggtcc agcgcgagat ttccaagcaa cattgcttct ctttcggcac
    10981 ggcggtccgt acggaaattc aaaatggatg gggaagagaa ggcgcttttg ctccttctgc
    11041 ggatgaacta atgcagatgg gatcagacca aatattccat ttgttcaaat agatatacat
    11101 cctgtgaggg gtcgtggttt cccatttgca ttacttcttg agagcgtcaa tatgtgcttg
    11161 acgagggggt tgagggaagg gcacaatctt gtctgaggct gtactttttg cttgaagaaa
    11221 actcataacg aaaggggtgg agatatgcat acctgtagtc atgccgttgt tggatgtatg
    11281 gattttcgta tccagaaaac cgttagcaac ttgctcgagc atctgaaggt ccacgaggga
    11341 acgtttgacc gcgtgacgtt tgccggaggt gccgccaaca aggagcaatt gaagcatcac
    11401 ctcgagcttt ccaaaagact tcatgacagc ggaatggcca ttctgtccgt gcatgaagat
    11461 tgcggtgccg gtgctaaaaa ggaagatctt tacgaggcgg cggaaactgc ccgctcactg
    11521 ggatatgacc cgaaaatgtt ctacatcaag cttgacggta cttgggaaga agtaaagcct
    11581 gcgtgaggga gtaaaggcag ctgagagcga acctaatgat ggaatgaagg gatagtttga
    11641 aactttgatt actgaataat gacgccctta cacggggcgt cattcactat tcattttatt
    11701 ttttctgcgc caatctttcc catgcttgag ctaccgtcat atccgttctt ggatgctggg
    11761 caagtttgga taatccatgg cgccgcagca attgataaac cgtcgacgtt gaaatctgcc
    11821 ttcccactcg cctctcaagc tcagctttca attgcggtat atgaagaacg ccggtgtctt
    11881 ttgcctcgct gacgaatgga gccaggaact tgacctcttc gtccaggctc atccgggcgt
    11941 ggtttcgtaa accttttttt gtttttactt gctcaacgcc tgtttcgatg cgctgaaatc
    12001 gggttcgaag cttgcccgtc cggctcttgg acaacccgat gatcatggcg gtctgttcca
    12061 gcgatagccc aaactcgagc ggaagcagta atgcctgagc agcgcgcaag gcatcaactg
    12121 ttttcgccga tgcgattctt tccttagcgg tctccacaaa ttcccgtccc gtcgcaggtc
    12181 ttgccatttg tatgtacact cgtatccaag tttaaatatg aatgaatgca gcgccttttc
    12241 acgccccgtt cccatgatag ccccacctcg tcacgttgcg ccagatcaca aggacgcttc
    12301 ctgacgcaat gccgccgtgt gatcggctcc tcggagcggg caaacaagca gtctgctcgc
    12361 ctccagggag ctccggatga aatgaagcac gatcaatacg acactgctgg tcctattacc
    12421 cattggcaat cggaaaccag gcacatcccc ccaaagtcct ttagcctggg tcgaattgcg
    12481 ttgatgactc tctcggcctg gttctcctca caagcaagga tcacaaccgc attcttttcc
    12541 tgcagtgcgt aatcatcggg gtggcgttct ccccgggtcc ccagcccgcc agcatgcttg
    12601 atgaaggtat agccccgcac attcactgac ttgaataatc caatcaagtc cccaagcgat
    12661 tcttcttcga taacgatctc gatccgtttc attgctatta aagcgtctgt tctcataatc
    12721 gtgactccct ctcaaaatat ttccacttat taggaacctg aatctttcga attggcggcg
    12781 cctgttcgaa agattcagct aattggcatc atcgtgtcga aagctgcacc aaggctatta
    12841 cgccgccccc tgccccgcca gatactgagc gatggcgtaa taggtgggga cgccgatcac
    12901 gatgttgaac gggaaggtca cgccgagcga caaggtgagg tagaaggagg gatttgcctc
    12961 cgggatagcc aggcgcatcg ccggaggcac tgcgatatag gaacagcttg ccgcaagcac
    13021 ggttaccagc gtgacgcctc ccactgaata ttggagaaag tagtggccaa ccaacatgcc
    13081 gaacgtcgcg ccgatgatgg gcatcactac accaaacgca accaggaaaa cgcccaccgt
    13141 tttgaattcc gatattcttt ttccggcttc catgcccatg tcacacagga atatgcacag
    13201 cgcgcccatg aatacgcctt caaagaacgg ctcgatcttc ttgtagctcg catccgaaac
    13261 cacgtagccg attcccatgg acaggaacag cagcaaaata ctgccgttgg tgaaggcttc
    13321 cctcacgaga tgaccataca tccccttctc gcccttttgg gcgccaccca tgatcttgcg
    13381 ggaataacct gccaggacga gaccgatcat gatcgcggga gattccatga tcgccagcat
    13441 gatgacggga tacgcctcat aggtgacacc catgctgccc aacatggcaa ctgcggtcat
    13501 gtaagtcccg gcgctcaccg agccataatg cgcagagatg gccgccgcgt ttagcggatc
    13561 aatcttccca acaccccgca aaatgatgta agccaggatc ggcaagaaaa tcccgaaaaa
    13621 gaatgctgcg gccaccgcgg gcatggcatc accgatcggc gaattgatga acgctctgcc
    13681 cccgtgcagc ccgatgccga tcagcagata gatgacgatc atcttgtgca tgtcgggcgg
    13741 gaacttcaga tccgatttga taaggcacgc gaacatgccg agggcgaaaa acagtatcgc
    13801 cggtacgaga agactagaca tttatatttc ccctttagtg agttggtgaa ctgcttggat
    13861 ggttacaaac acgcttgcca gaactgcccg ttctgagcct cccgaagcca ggggtcggag
    13921 gtccgaacgg cgatgagccg tcaggaagag cccggcggcc cggcccggac agtatcgccg
    13981 ggaccctgag cattccggaa accgccctgt cgacctgcgc acgccccctt tttgggggat
    14041 ggcccaagtc ggcctcctcc aatatctcat gcgcgctaat ccctcttttc cttttcaggc
    14101 aaaaataagc atttatcata taccttcccc atcacggtaa aaatctggaa cagaactgga
    14161 attaataaca actactgatt cagctggaca ggtagtctgg acgcatacgt tttatgtcca
    14221 ttcccgctat cgctcaatca cctttcaacc ctacgataat ccttcgcggc ttatcgcggc
    14281 tctggctcca agttatatct tctttacgtg tatctctaag tcgctctcat tttcaaccca
    14341 ctgaaactga taaagatttt ctactgaata gaaacattat ttcgttttag agggtgtgaa
    14401 tttcaggtta gggcaaaaat taacataccg gttgtgaaga aaatattttc tatctgtgat
    14461 gaatttgtga aagctacaat aaagattcca gatcgtgaaa aaactgtcct tccgatccaa
    14521 atgctcctgg ctattaaggt tccgtttatt tatagaatat ggcttggaat agcaacaaat
    14581 ataaagaggt tttccggcga aacctgatca cgggtgggat tcatttgcca tagaggaaga
    14641 agaggtattc aggcgctgcc gggggtagct gtggaaacca ctgataaagc cggggaaact
    14701 gccgttgatc ccgacttgaa atatgctgaa gtgcaggtaa gttgttacga gggaatcaga
    14761 agtgaaggct tcagacgtcg ttttgcttgt gcaattgggg gctggcagtg gacggccgcg
    14821 caggcgccga ccgtattatt gttgcttggt attctttctg gctctgacga tgcgcttcca
    14881 gagcaaactg gggtgtggcc accggctatc accaaggcaa gcggtggtcg agtaatttgt
    14941 gatagagcgg ttgtcgtaaa gccgtcagca aggcgcgcac ttcgattgca cctgtcgatt
    15001 tcctccatat tctccaatcg atccataacc ttttgtcata ctttttccgg ctgtagaaac
    15061 gtatcgaaac aaaaacggga gttaataacg actgaagcag gcggaatacc ccctatccat
    15121 acgaggcctt ttattcattt tgtttaaccc gccattcctc ttgccaaagc cgcttttttc
    15181 cccagttaac cgtgatcatt tcggaattaa ttctctatcc accgttgtct aatgcctgaa
    15241 ccttttatat ttgtctgaat attaatcaaa gtttttacta cactgctgat cattcctgtt
    15301 acaacattcc gtaattacgc ttgggggcgc tgagattcgt aaatgtggtg tgaagaaaac
    15361 attcttgaag aaaatattct ctatttgtga tttatttgtg aaagcgagtt gcctcaagct
    15421 ctgtgcgttc ggcatgcttc ggtttgccat gccgctcacc ccggatgcct cagtggcata
    15481 ggcttgggtg ctgggcactc tcggcgccat cacattcctc tacacggcaa tgatcgcctt
    15541 gcaacaaaca aatctcaggc gcctgctggc ttacgccagc gttgcacatc gggctggtaa
    15601 tcgtcggttc caggcccgag cggatttccg gcagaactgc tcttgattat
//
LOCUS       DASSHJ010000166         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_658430, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000166 DASSHJ010000000
VERSION     DASSHJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658430"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..481
                     /locus_tag="VFI43_06410"
     CDS             143..481
                     /locus_tag="VFI43_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7061795.1"
                     /translation="MKKIEAVIKPFKLDEVCEALNEIGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKIEIVVDDKLVDNIIDMIVNTARTGKIGDGKIFVTTVEQVVRI
                     RTGETGDPAV"
     gene            620..2131
                     /locus_tag="VFI43_06415"
     CDS             620..2131
                     /locus_tag="VFI43_06415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7061796.1"
                     /translation="MNFRLLPVVLALLFIPPVNSSELPDLGDVSQAALSTREERDLGL
                     QIMAEIRADKDYLDDAEIAGYLTTLGNKLILGSREARPDQDFEFFTVKDPAINAFALP
                     GGFMGFNTGLILAAQSESELAGVMAHEIAHVTQKHLARMIAGQKYNLLTTLAALALAI
                     LASRTNPQVSEAVLVGSQARQIQSQLDFTRDHEKEADRVGMNILVSAGFDPHGMASFF
                     EHLQKAGRFHENGAPSYLRTHPITYERIADIENRIQQTSYRQVPDSIDFQLVRAKVRS
                     YLEKPGDAVKYFDSALRDKRYSNEAVERYGLVNALLEDKKYVRADKELALLYETLNAR
                     AIDETLEDHKLGAAIRIQRKAVPSSPMIETLAARVKLSVGQTAEALDLYRAALKIYPQ
                     HRALVYDYAGALLRTGRADTALKFISQQLRFTPEDIRLYSLQAQAYEAMGDHMSQHRA
                     QAEVYARQGNLTSAIEQLQIALRANDPDFYQASSMEARLRELREMAANKPKSK"
     gene            2257..3402
                     /locus_tag="VFI43_06420"
     CDS             2257..3402
                     /locus_tag="VFI43_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7061797.1"
                     /translation="MVLTLSRRTFVVILALLTTAAGFWYWIVKNESRPERYKTQPLER
                     GDIVHSISANGTLNPVVLVNVGTQVSGTVYKLFADFNDRVEKGEILVELDPALFEAQL
                     GQSKAAVLNAEVALRVAENKMARNVALKEQGFISPDALDVVVQQLETARAQLAVNRAQ
                     LERDRTSLNYTIIRSPISGVVIARNVDIGQTVAASFQTPVLFQIAKDLKQMQIDTSVA
                     EADIGHLQLGQPVTFTVDAFQDREFTGVVEQVRLNPTIQQNVVSYNVIVKVANDEGLL
                     LPGMTANVRFTINRKKAVLRAPNAALRFRPSGEEADSAVSTKLAGSQMLYLLEEGKPA
                     AVNVSTGASDSNHTEILGDNVKEGDKVIVREVGEKEKSGSKLKFRMF"
     gene            3402..4130
                     /locus_tag="VFI43_06425"
     CDS             3402..4130
                     /locus_tag="VFI43_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061798.1"
                     /translation="MESSRASSSPLIYVGDLCKIYTLGNGASGTINSQVLFDINLVIE
                     RGEFVAIMGHSGSGKSTLMNILGCLDTPTSGHYWLDGQDVSSLSDDDLARVRNRNIGF
                     VFQGFNLLKRMSALDNVATPLLYAGASRSASRKRALELLNQTGLGKFAGYQPNQLSGG
                     QQQRVAISRALVNNPQLILADEPTGNLDTQTSREIMDVFQRLNREQGITVVLVTHEDD
                     IATFTRRLVRLQDGRVVHDGGVTA"
     gene            4127..>4986
                     /locus_tag="VFI43_06430"
     CDS             4127..>4986
                     /locus_tag="VFI43_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7061799.1"
                     /translation="MSPLAIIGEALRALRLNRLRTGLTMLGMVIGVAAVVLMLAVGQG
                     AQTTVNQAITSMGSNLFFIVPGATSSGALRSGAGAVQTLTLGDAYAIAQLPSIKATAP
                     VITGTAQLNYGANNWSTSVTGATPDYFTVRDWPAENGALITEADLRSSNRVVVLGQIT
                     ANNLFGDEDPVGKMVRIKSSPFLVAGVLAAKGQSLDGRDQDDAVFIPLTTGQRQVFGN
                     QFPGTIRFMMAQAQSAEVMEEAEIEMNRLLRQRHRLAEGTENDFTVRNLAALASVATG
                     AAKVMSLMLGA"
BASE COUNT         1168 a         1348 c         1398 g         1072 t
ORIGIN      
        1 tttacaagac tgccgtccag cctgccgact tgagtacttg agtaaatgac tggttttctc
       61 tggatcaact cgcttcgaat ggagtttcca gattcagtag cggcatggct agcgaagcaa
      121 gtccaaccct ggggcgatta ccatgaaaaa aatagaggcc gttatcaagc cattcaagct
      181 tgatgaagtg tgtgaagcgc ttaatgaaat tggcgtgagt gggctgacag tcaccgaggt
      241 aaagggtttc ggacggcaga aaggacacac cgaactctat aggggcgcgg agtacgtggt
      301 cgatttcctc cctaaaataa aaatcgagat cgtggtagac gacaagctcg tcgataatat
      361 tatcgatatg attgtaaata ctgcgcgaac aggcaaaatc ggcgacggaa aaatcttcgt
      421 cacaacggtt gaacaggttg tgcgtatacg aaccggcgaa acgggtgacc ctgcagtgtg
      481 acaatcattt gccctgatcg atggctggaa gcagccagga ttcaactggg gaagatttca
      541 cggtattttc acgaattgaa gcgctgagcg acgtcacggt acaggttgaa ctttgctcca
      601 gccgctccat cttacccaca tgaattttcg tctgttgccg gttgtcctgg cgttgctgtt
      661 cataccacct gtaaactcga gtgaactgcc cgatctgggg gatgtttcac aagccgcgct
      721 gtccacgcgc gaggaaaggg atctcggcct tcagatcatg gctgagattc gtgccgacaa
      781 agactacctg gacgatgcgg agatcgccgg ttatctcacg actctgggca acaagttgat
      841 tctcggctct cgggaagccc gtcccgatca ggacttcgag tttttcacgg taaaggatcc
      901 ggcaatcaac gcattcgccc ttccgggtgg attcatgggt ttcaatactg gcctgatcct
      961 tgcagcgcaa tccgaatcgg aactggcggg tgtgatggcc catgaaatcg ctcacgtcac
     1021 gcagaagcat ctcgctcgca tgatcgccgg gcaaaaatat aatctgctga cgaccctggc
     1081 cgcattagcc ctggcgattc tggcgtcccg aacaaacccc caggtaagcg aggctgtttt
     1141 ggtcggctcg caagcacgcc agatacaatc ccagctcgac ttcacacgcg accacgaaaa
     1201 ggaggctgat cgagttggca tgaatatact ggtcagcgcc ggcttcgatc cgcacggcat
     1261 ggcttccttt ttcgagcact tgcaaaaagc ggggcgcttc cacgaaaacg gcgcgccttc
     1321 ctaccttcgc acccacccga tcacctacga gcgcatcgcg gatatcgaga atcgtattca
     1381 acaaacctct tacaggcaag tgcctgacag tatcgacttc cagcttgttc gggcaaaggt
     1441 ccgttcgtat ctcgaaaaac cgggagacgc ggtaaaatat ttcgattccg cgctgcggga
     1501 caagcgctat tccaatgagg ctgtcgagcg atatggactg gtcaacgcgt tgcttgaaga
     1561 caagaaatac gtgcgggccg acaaggaact ggctctcctg tacgaaacct tgaatgcgcg
     1621 agcgatcgac gaaaccctgg aggatcacaa gcttggggcg gctatccgca ttcaacgcaa
     1681 ggccgtacct tccagcccaa tgattgaaac actggctgcc cgggtaaaac tttccgtcgg
     1741 tcaaacggcg gaggccctcg atctttaccg tgccgcgcta aagatatatc cccagcatcg
     1801 ggcgctcgtc tatgattacg ccggcgcact gctgcgaacc ggaagggcgg acactgcgct
     1861 gaagttcatc agccagcagc tgcggttcac accggaagat ataaggctgt attcactgca
     1921 ggcgcaagca tatgaggcga tgggagatca tatgtctcaa catcgcgctc aggcggaagt
     1981 atatgcgcgg cagggtaatt tgacgtctgc aatcgaacag ctccagattg cattgagagc
     2041 gaacgatccc gatttttatc aggcatccag catggaagca cgactgagag agctgcggga
     2101 aatggctgcc aataagccga agtccaaata agtcttgata tcgccttttc ttcacccgct
     2161 cttgccgatc tccttgagcc tcgggtccga ccctctgcag tcaggtaaca ttttcgattt
     2221 tgcccggcag gacgcggcac tcaaccctgg gtttgtatgg tcctcacctt gagccgcagg
     2281 acgttcgtcg tcatcctcgc cttattaacg actgctgccg gcttctggta ttggatcgtc
     2341 aaaaatgaat cgagaccgga gcggtacaaa acccagccgc tcgaacgcgg cgatattgtt
     2401 cacagcatct cggcgaacgg cacccttaac cctgtcgttc tggtcaatgt cggtacccag
     2461 gtatcaggaa cggtctataa gctctttgcc gactttaatg accgtgtcga gaaaggggag
     2521 atcctggttg agctcgatcc agcactgttt gaagcccagc taggccaatc gaaagccgcg
     2581 gttctgaatg ccgaggtcgc gctgagagtc gccgagaaca aaatggctcg caacgtcgcg
     2641 ctgaaagagc aaggctttat ctcgcccgac gcgctggacg tcgtcgtaca gcaattggaa
     2701 accgcccgtg cgcaacttgc ggtcaacagg gcgcagctcg agcgggatcg gacaagcctc
     2761 aattatacga ttatccgatc acccatttct ggagtagtta tcgcacgcaa cgtcgatatc
     2821 ggccagactg tcgcagcgag ctttcagacg cccgtcttgt tccagattgc gaaggatttg
     2881 aagcagatgc agatcgatac gagcgtcgct gaagccgata ttggtcatct gcagctcggc
     2941 cagcccgtaa cattcacggt ggatgccttc caggatcgcg aatttaccgg cgtagttgag
     3001 caggtgcggc tcaacccgac gatacagcaa aatgtcgtgt cttataacgt cattgtgaag
     3061 gtggcaaatg atgagggctt attattgcct ggcatgaccg ccaatgtacg ttttaccatt
     3121 aaccggaaaa aagcggtgct gcgcgcccct aacgcagcac tccgcttcag gccaagcggc
     3181 gaggaggcgg actcggcagt ctcgacaaag ctcgcaggca gtcagatgct ttatctgctc
     3241 gaagagggca agcccgccgc cgtgaacgtc agtaccgggg cgtcggatag caaccatacc
     3301 gaaatcctcg gggataacgt caaggaaggg gataaggtaa ttgtccgaga ggtcggcgaa
     3361 aaggaaaaat ccggcagcaa gctcaaattc agaatgttct gatggaatca tctcgcgctt
     3421 cgagttctcc cctgatatac gtgggagatc tgtgcaagat ttataccttg ggaaacggcg
     3481 caagtggaac aatcaatagc caggttcttt tcgatatcaa ccttgttatt gagcgtgggg
     3541 agttcgtcgc gataatgggt cattccggtt cgggtaaatc cacgctgatg aatatacttg
     3601 gctgcctgga cacgcctacg agcgggcatt attggctcga cggccaggat gtctcatctc
     3661 tttcggatga tgatcttgcc cgcgtccgga atcgtaacat cggcttcgtg ttccagggct
     3721 tcaacttgct gaaacgcatg tcggcgctgg acaatgtagc cacccccttg ctatacgcag
     3781 gcgcaagccg ctctgcaagc aggaaacgcg cgctggaact tctcaaccag acaggcctgg
     3841 gtaaatttgc aggctatcaa ccgaaccagc tttccggcgg gcagcaacaa cgcgtggcca
     3901 tcagccgggc cttggtcaat aatccccagc tcatacttgc tgatgaacct accgggaatc
     3961 tcgatacaca aaccagccgc gagatcatgg acgtgtttca gcggcttaac cgggagcaag
     4021 gtattaccgt tgtgctggtt acccatgagg atgatattgc cacttttacc cggcgcctgg
     4081 tccgcttgca ggacgggcgt gtggtgcatg acggcggggt gacggcatga gtccgctcgc
     4141 gatcatcgga gaggcgttgc gcgcgctgcg cctcaaccgg ttgcggacgg gcctgacaat
     4201 gctcggcatg gttattggcg tggctgcggt cgtgcttatg ctcgccgttg gccaaggcgc
     4261 acaaaccacg gtgaaccagg caataacctc catgggcagc aacctgttct tcattgtgcc
     4321 tggtgcaact tcttccggtg ctctgcgctc cggggccggg gcagttcaga cattgaccct
     4381 gggggatgca tatgccatcg cccaactgcc ttcgataaag gcaaccgctc cggtcataac
     4441 aggaacggcc cagctgaatt atggcgcgaa taactggagt acctctgtta cgggcgccac
     4501 gcccgattac ttcaccgtac gtgactggcc ggcggaaaac ggcgcgttga tcactgaggc
     4561 ggacctgcgg tccagtaacc gtgtcgtggt tctcggacaa ataacagcga acaacctgtt
     4621 tggtgacgaa gacccggtag gaaaaatggt gcgcatcaaa tccagtcctt ttctggtggc
     4681 aggggtactt gccgccaagg ggcagagcct ggatgggcgc gatcaggacg acgccgtatt
     4741 tattcccctc acgaccgggc agcgccaggt attcgggaac cagtttcccg gaacgatacg
     4801 cttcatgatg gcgcaagccc agtcagcgga agtcatggag gaagcggaaa tcgaaatgaa
     4861 cagactgttg cggcagcggc accgccttgc ggagggtacg gagaacgact ttaccgtgcg
     4921 caatctggcg gcactcgcct ccgttgcaac cggcgctgcc aaggtcatgt cgctcatgct
     4981 cggcgc
//
LOCUS       DASSHJ010000167         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1092329,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000167 DASSHJ010000000
VERSION     DASSHJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092329"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /gene="purH"
                     /locus_tag="VFI43_06435"
     CDS             complement(<1..346)
                     /gene="purH"
                     /locus_tag="VFI43_06435"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111487.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7061800.1"
                     /translation="MNVKQALISVSDKRGIVEFARELRQLGIKILSTGGTARLLKEAG
                     VEVTEVGDYTGFPEMLDGRVKTLHPRIHAGILARRELPGHVAALEAAGIPGIELVVVN
                     LYPFSRTISRSDC"
     gene            complement(427..669)
                     /locus_tag="VFI43_06440"
     CDS             complement(427..669)
                     /locus_tag="VFI43_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7061801.1"
                     /translation="MSIIDENEIARCVRKAIEEYFNDLGGEKPHAIYDMVIRSVEKPL
                     IEMVVEQAGGNQTRAAELLGINRNTLRNKMKQYQIK"
     gene            complement(666..1679)
                     /gene="dusB"
                     /locus_tag="VFI43_06445"
     CDS             complement(666..1679)
                     /gene="dusB"
                     /locus_tag="VFI43_06445"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7061802.1"
                     /translation="MQIGSHILKNNLIVAPMAGVTDRPFRQLCKSMGAGMAVSEMVSS
                     NSLLWGSEKTRRRANHEGEVDPISVQIAGADPAMMAEAARYNVTQGAQIIDINMGCPA
                     KKICNVMAGSALLKDQPLVGRILDAVVGAVKVPVTLKIRTGWDTQNKNAVTVARIAES
                     AGVQALAIHGRTRACAYAGEAEYDTIAAVKALVKIPVIANGDITTPEKAKHVLEHTRA
                     DAIMIGRAAQGRPWIFREISHYLETGTHLPLPEVVEIHRVLVEHLHELYDFYGEYSGV
                     RIARKHISWYTKGLKGSASFRHAMNQLQTIDQQLDAVNEFFGELAGYGRRLTYEESEE
                     LVA"
     gene            complement(1856..>2116)
                     /locus_tag="VFI43_06450"
     CDS             complement(1856..>2116)
                     /locus_tag="VFI43_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiH"
                     /protein_id="HET7061803.1"
                     /translation="RDAWELAEEAISSPTDIGTPAMLERYRERRKMDSGVGRTFTDSL
                     VRLFSNDNVVLGLARGLGLTALDCLPPAKQFVARRMMFGPRG"
BASE COUNT          433 a          621 c          566 g          496 t
ORIGIN      
        1 tgcaatccga gcgggatatg gtccggctga aaggataaag gttgacgacg accaattcga
       61 tcccgggaat cccggcggct tccagcgccg ccacgtgtcc gggaagttcg cgccgggcaa
      121 ggattcccgc gtggattcgc ggatgcaggg ttttcacccg cccgtcgagc atttccggaa
      181 accccgtgta atcgcctacc tcggttactt ccacgcctgc ttctttaagc agcctggcag
      241 ttccccctgt cgagaggatt tttatcccta actgacgcag ctcgcgggcg aattcgacaa
      301 tgccacgctt gtcggaaacg ctaatgagag cctgtttaac gttcatcaat cgattgtttt
      361 tattaagggg gaacagcagc gccctttgat ccggctttgc tgagccagat ccagggctga
      421 gggaatttat ttgatctggt actgcttcat tttgttgcgc agcgtgtttc ggttgattcc
      481 gagcaattcc gccgcgcgcg tctgatttcc gccagcctgt tcgacaacca tttcgatgag
      541 cggcttctcg acgctacgta ttaccatatc atagatggcg tggggctttt ctccaccgag
      601 atcgttgaaa tactcttcga tggccttgcg cacgcaccgg gcgatctcgt tttcatcgat
      661 gatgctcatg ccaccaactc ctcggactct tcgtaagtca acctccgccc gtacccggca
      721 agttcaccga aaaattcgtt gaccgcatcg agctgctggt cgatcgtctg caactggttc
      781 atcgcatgcc tgaatgatgc ggagcctttg agacccttcg tgtaccagga gatgtgcttg
      841 cgagcaatgc gcacgcccga atattcaccg taaaaatcat aaagctcgtg cagatgctcc
      901 acgagaaccc ggtgaatttc cacgacctcg ggcaggggaa gatgagttcc ggtttcgaga
      961 taatggctga tttcgcgaaa tatccacggc cgcccctggg cagcccgccc gatcatgatg
     1021 gcatccgccc gggtgtgctc aagaacgtgc ttcgcttttt ccggggtggt gatatcaccg
     1081 ttggcgatga caggtatttt cactaacgcc tttaccgccg caatagtgtc atattctgcc
     1141 tcaccggcat aggcacaggc gcgagtgcgt ccgtggatgg caagggcctg cacaccggca
     1201 ctctcggcaa tgcgagccac ggtcaccgcg tttttattct gggtgtccca gcccgtgcga
     1261 atcttcagcg tcacggggac cttgactgcg cctaccacgg cgtcgaggat tcgtccgaca
     1321 agcggctgat ccttgagcaa cgccgagccc gccatcacat tgcagatttt cttcgccggg
     1381 cagcccatgt tgatatcgat gatttgcgct ccctgagtca cattgtaacg agccgcctct
     1441 gccatcattg cgggatcagc ccctgcgatc tgaacggaga tgggatcgac ttcgccttca
     1501 tgattggcgc gccgccgggt cttctccgat ccccacagca acgaattgct ggaaaccatt
     1561 tcggagaccg ccatgcccgc ccccatgctc ttgcatagtt ggcggaaagg ccggtccgtg
     1621 acgccagcca ttggcgctac gatgaggttg ttcttgagga tgtgcgagcc gatctgcatg
     1681 acagtaattt agggcgccgc aacgcgggta aaaggaacag ctataatctt aacagaaaaa
     1741 cccctaccgc ttacaggggc agcaccctcc tcaacagggt tggttttacg gctcgctttt
     1801 accgcttttg aataatttgt cgagaattcc cgttttaggt gttttcaggc cgaaatcagc
     1861 ctctcggccc gaacatcatg cgacgcgcaa caaactgctt ggctgggggt aagcaatcga
     1921 gagcggtgag tccgaggccc cgcgccaatc caagcacgac attatcgttg gaaaacaatc
     1981 ttaccaagga gtcggtaaac gtgcgcccga ccccgctatc catcttgcgc cgctcccgat
     2041 atctttccag catcgctgga gtcccgatgt cggtgggaga ggaaatcgcc tcttcggcga
     2101 gctcccatgc atcacg
//
LOCUS       DASSHJ010000168         9041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_339381, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000168 DASSHJ010000000
VERSION     DASSHJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339381"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..2296
                     /gene="prsK"
                     /locus_tag="VFI43_06455"
     CDS             215..2296
                     /gene="prsK"
                     /locus_tag="VFI43_06455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HET7061804.1"
                     /translation="MAPGIAAISYTMAGAANLFLATLLLTRWRSRSSPIVLPAACLLS
                     AVWGFGLAYHATQNPSSSLAANILEILRNAGWSMFIVLLLGLYRQGSSPAISRISPAA
                     LALGTLYICCLVLVVYVQAEPSFSSTDDPDFLNNIASLAMAITGMILVEQLYRNTPVK
                     QRWGIKFACLGIGGIFAYDFYLYSDALLLRHINPEIWAARGTINALVVPLIAVSAARN
                     PQWSIEITVSRRILFYSAALFGAALYLLAMAAAGYYFRFFGGSWGTVMQVTFLFGALI
                     LLLAVLFSGTLRSWLRVFISKHFFSYNYDYREEWLRFTRTLSEGGQGLEERALQALAQ
                     LVESPAGALWVCRESGKCEPVAQLNINAGSGLEPVDSPFCRFMIQKEWVIDLEEYAKS
                     PEKYSGLTLPQWLQRLPRAWLVVPLIQHRKLFGFVVLAQPRSRVKLNWEVSDLLKVAG
                     NQAASYLAQHEAANALMIARQFESFNRMSTFVVHDLKNLVSQLSLLLSNAEKHQNSPE
                     FQQDMVETVYLSVQKMKRLLEKLSSASSPEKPAPLFIAPLLRETVKSKSIVEPKPILE
                     IQDSGLEVCANSSRLERVMGHLIQNAIEATSRSGHVWVRLKRKNDFAMIEIRDTGRGM
                     SQEFIREKLFKPFESTKSAGMGIGVFESREYVSELGGEMEVSSSESEGTVFRIALPLH
                     RGEQTAVERVG"
     gene            2329..3681
                     /gene="prsR"
                     /locus_tag="VFI43_06460"
     CDS             2329..3681
                     /gene="prsR"
                     /locus_tag="VFI43_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HET7061805.1"
                     /translation="MGQDKKVLLVIEDDPGLQKQLRWSFDAYEVLVAGDREGALALAR
                     RHEPAVITMDLGLPPDPDGATEGLALLEQLLDLAPDTKVIVLTGNQDPGHALKAIGIG
                     AYDFHQKPFDPQILGLVVERAFFLHALQQENRRLLQAQIPSPSGGIVTRDPALVKVCR
                     NIEKVAPSDATVILLGESGTGKEILARTLHLSSARKEKRFMAINCAAIPEALLESELF
                     GYEKGAYTGAAKQTPGKIELAHEGTFFLDEVGDLPMALQAKLLRFLQERVIERVGGRK
                     EIHVDVRIVCATHQNLKKLIEENRFREDLYYRLSEIVITIPPLRARVGDAALLAHHFR
                     NKFCNQEKRQLLNFSKEALAAIESHGWPGNVREMENCIKRAVIMADGPLITADDLGLR
                     EASAEAEPINLRQVREKAEYDALMKALARVDGNVVKAAELLGVSRPTIYDLMSRHQIK
                     "
     gene            3713..5308
                     /locus_tag="VFI43_06465"
     CDS             3713..5308
                     /locus_tag="VFI43_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HET7061806.1"
                     /translation="MTVLIHELIQCSASRFPDSEALVHKKNRISYSAMAREVDLAKNS
                     ILDLGLDSGERVAVYLEKRPETVIALFGATAAGGVFVPVNPLLKAEQVAYILRDCNVR
                     ILVTSVERLRLLAGVLEHCHDLRTVIVVGREAPPVTVRHANLKFVQWGEALAQAGAIR
                     AHPRIDNDVAAILYTSGSTGKPKGVVLSHRNLVAGAASVSEYLKNRSDDRILSVLPLS
                     FDYGLNQLTTAFHVGATAVLMNYLLPRDIVDLVRDENITGLAAVPPLWIQLAQLKWPD
                     NLPLRYITNSGGAMPRATLELLRSHLPGTAVFLMYGLTEAFRSTFLPPEQVGIRPDSI
                     GKAIPNAEVLVVREDGSLCAPGEPGELVHRGVHVSMGYWNDPEKTAERFRPVPSRQPG
                     LTIPELAVWSGDTVRMDEEGFLYFIGRRDEMIKTSGYRVSPMEVEEVIYATELVAEAA
                     AVGVRHPVLGQAIALIATPRAGRVPDEQALLSACKHNLPAYMLPSRVIYKEGSLPRNP
                     NGKIDRKLLADELQNVFMETVNE"
     gene            5301..6536
                     /locus_tag="VFI43_06470"
     CDS             5301..6536
                     /locus_tag="VFI43_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HET7061807.1"
                     /translation="MSDMRPKHAAMLQFPVEDDCLQVGGIPLTRLAQRIGRTPFYAYD
                     RRLVSERVAWLRHCLPEGIHIHYAMKANPMPALVQHMAHLVDGIDVASAGEMKVALDT
                     PLAAENISFAGPGKRDDELGCAIAAGIVINLESEQEMERVSALAERQHVRPKVAVRVN
                     PGFELKSSGMKMGGGAKQFGIDAERVPAVLGRIGQLGLDFEGFHIFSGSQNLKVASIQ
                     EAHEKTFALAIALAQHAPGEIRLVNIGGGFGIPYFPGDEPLDLKAVGDNLRQLLPEAK
                     RRLPEAQVVVELGRYLVAEAGVYVCQVLERKKSRDKIFLITDGGLHHHLAASGNFGQV
                     IRKNYPVIVGNKVNGAARETVSVVGPLCTPLDLLADQMEMGQAEAGDLIVVFQSGAYG
                     LTASPTAFLSHPVPVEVLV"
     gene            6656..8512
                     /locus_tag="VFI43_06475"
     CDS             6656..8512
                     /locus_tag="VFI43_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET7061808.1"
                     /translation="MSGFCGWIGQGTSVPENLQLVRRMAEPISRFDSSSVKSISGKRS
                     AAAVAAPEGRAHLYQKEGLTVAIRGRPRFMDERQDPVETCEGIAKSVAEQWRNRRAEV
                     FTGLSGAFSLCILDEDSTEAVLATDRMGTYPVSFQLSGQALVFGSTADSVIRHPSIKP
                     EIAPQSLYNYVYFHMIPGPDTIYQGQNRLLPGEYLVYRKGRIETRKYWEMQFVESETR
                     PFQELRENFLGVLRSSVRTAVDGEEVGAFLSGGTDSSTIAGILGEVTGQPARTYSIGF
                     DASGYDEMSYARIAARHFGTRHQEYYVTPDDIVTAIPQVAGIFDQPFGNSSAIPSFYC
                     ARMAKADGLARMLGGDGGDELFGGNSRYAKQHLFSLYERIPALLRKALIEPMVFGIPQ
                     RVAPPMLRKARSYVEQASIGMPARTETYNLLEHYGAVEVFSREFLETVDPAYPGILLS
                     EIYHQNPAESLINRMLAFDRKFTLADNDLPKVSKACELAGMDIAYPLISDEIVDFSLR
                     LEPQLKLKGTKLRYFFKEALRGFLPDEIITKQKHGFGLPFGVWLQDHKPLQTLASDSL
                     SDLKTRNIINREFINKLLGQHLGEHAGYHGTMVWVLMMLEQWYRQRAESVKV"
BASE COUNT         2013 a         2319 c         2612 g         2097 t
ORIGIN      
        1 atcattatcc tgatcgatac gatcgaagtc gtcctgttac gcaagggtgg acgatgaggc
       61 ggaagatccc ctcgtcattg atagacgtgt tcttcaagca cactgcgtct actgggaaat
      121 tccacagttc cccccttgga aaggaggggt cgagggctcc cgcaggacgc cgttgttttt
      181 tcatgcttgc tgttgttgcc ggtctcgtcg tgtgatggca ccgggcattg cggcaatcag
      241 ctacacaatg gcgggcgccg caaatctttt cctggcaacg ctgctactga ctcgctggcg
      301 gagccgttca agtccgatcg tactgcctgc tgcttgcctt ctctccgccg tatggggctt
      361 cgggctcgcc tatcacgcaa cgcaaaatcc ttcttcctcg cttgcggcaa atatcctgga
      421 aattctcagg aacgcgggct ggtcaatgtt tatcgtttta cttctggggt tgtaccggca
      481 gggttcatct cccgcaatct caagaatcag tcctgccgcc ctggcccttg gcacgctcta
      541 catttgctgc cttgtgctcg tcgtatacgt tcaagccgag ccaagctttt cctccacgga
      601 cgatccggat tttctcaata atattgccag ccttgcaatg gcgataacag ggatgatcct
      661 ggtggagcaa ctttatcgca acaccccggt caaacagcga tggggcatca aatttgcctg
      721 tctcggaata ggaggaatct tcgcctatga cttttacctg tacagcgacg ccctgttgct
      781 gagacacatc aatccagaga tatgggccgc acggggaacc attaatgctc tggttgttcc
      841 gctgatcgcg gtatcggcgg cacgcaaccc gcagtggtcg atcgagataa ctgtttctcg
      901 ccgcatcctc ttctattccg cagcattatt cggggccgcc ctgtatctgc tggcaatggc
      961 tgcagccggt tattatttcc ggttcttcgg gggaagctgg ggtacggtca tgcaggtgac
     1021 attcctgttc ggggcgctga tcctgttatt ggcggttcta ttctcgggca ctctacgctc
     1081 ctggctcagg gttttcatca gcaagcattt cttcagctac aactacgact accgggaaga
     1141 atggctgcgt tttacccgca ctctttccga gggggggcaa ggcctggaag agcgggcgct
     1201 tcaggcgctc gcgcaacttg tcgaaagtcc tgccggggcg ctttgggtgt gccgtgaatc
     1261 gggcaaatgc gagcctgtcg cgcaattgaa tatcaatgca gggagcgggc tggagcctgt
     1321 ggacagcccc ttctgccggt tcatgatcca gaaggaatgg gttatcgacc tcgaggaata
     1381 cgccaaaagt cctgagaaat actccgggct cactctgcct caatggttgc agcgtctgcc
     1441 aagggcgtgg ctcgttgtgc cattgatcca gcatcgcaag ttgttcgggt ttgtcgtact
     1501 tgcacagccg cgaagcagag taaagctgaa ctgggaggtg agcgacctgc tcaaggtcgc
     1561 cggcaaccag gcggcaagct acctggcgca gcatgaggca gcgaacgcgt tgatgatcgc
     1621 gcgtcagttt gaatcattca accgaatgtc cacgttcgtg gtgcacgatt tgaagaatct
     1681 ggtttctcaa ctgtcactgc ttctttcaaa cgccgagaaa catcagaaca gccccgagtt
     1741 ccagcaggac atggttgaaa cggtgtatct atccgtccag aaaatgaaac ggctgcttga
     1801 aaagctgagc tccgcgagtt ctccggagaa gcctgctccc cttttcattg ctccgttact
     1861 gagggagacg gtcaagagca aatccatcgt cgagcccaag ccaatccttg aaattcagga
     1921 ttccggtctg gaggtttgcg cgaattcatc gcgtctggag cgcgtaatgg ggcatctcat
     1981 ccagaatgcc atcgaggcga caagccgaag cgggcacgtt tgggtacgtc tgaaacgtaa
     2041 aaacgacttc gccatgatcg agataaggga cacggggcgc gggatgagcc aggaattcat
     2101 ccgggaaaag ctgttcaagc cgttcgagtc gaccaaatcg gcgggaatgg gtattggtgt
     2161 ttttgaaagt agagagtatg tctccgaatt gggcggggag atggaagtct ccagcagcga
     2221 atcggagggg acggtcttcc gtattgccct ccctctgcac cgcggtgagc agacagctgt
     2281 ggaacgagtg ggttgacaac attttgaaaa gaaactgtag aggttaaggt gggtcaggat
     2341 aaaaaggttc tactggtcat cgaagatgat ccgggattac aaaagcagct acgctggagc
     2401 tttgacgcct acgaggtact cgtcgcgggt gaccgtgaag gtgcgctcgc cctggctcga
     2461 aggcacgagc ctgcagtcat taccatggat ctgggcctgc cacccgatcc ggacggcgct
     2521 acggaaggtc tcgctttatt ggagcagctg cttgatcttg cgcccgatac aaaagtaatc
     2581 gttctgacag gaaaccagga tcctggtcac gctctcaagg cgattggcat aggtgcttat
     2641 gattttcacc aaaagccttt tgatccgcag attctcgggc tggtggtgga acgggctttt
     2701 tttctgcacg ctcttcaaca ggagaaccgg aggctgctgc aagcacagat tccctcgcct
     2761 tccggaggca tcgtaacgcg cgatccggcc ttggtaaagg tctgccgcaa cattgaaaag
     2821 gttgcgcctt ccgacgcgac ggtaatcctg ctgggcgaga gcggcaccgg taaggaaatc
     2881 ctggcaagaa cactgcatct ctcgagcgcc agaaaagaaa agcgttttat ggcgatcaat
     2941 tgtgcagcca ttccggaagc gcttctcgaa agcgagctgt ttggctatga gaaaggcgca
     3001 tacacaggag cagccaagca gaccccgggc aagatcgaac ttgcccacga gggaacattt
     3061 tttctcgatg aagttggaga tttgcccatg gccctccagg caaagcttct tcgcttcctg
     3121 caggaaagag tgatcgaaag agttggggga cgcaaggaaa ttcacgttga cgtgcggatt
     3181 gtttgtgcaa cgcatcaaaa tttgaagaaa ctcattgagg aaaatcgttt tagagaagat
     3241 ctctactatc gtctcagcga gatcgtcatc acgatccctc cattgcgcgc acgtgtgggt
     3301 gatgccgcgc tgctggcgca tcacttcagg aataaattct gtaatcagga aaagcgtcaa
     3361 ttgctcaatt tcagcaagga agcgcttgcc gcaatagaaa gccatggctg gcccgggaat
     3421 gtgagggaaa tggaaaactg catcaagcgc gcggtcatca tggcagacgg gcccctgatc
     3481 acggcggatg atctgggctt gcgggaagcg tccgcagaag cggaaccaat aaatctgcgc
     3541 caggtgcgtg aaaaggcgga atatgacgct ttgatgaaag cgctcgcccg ggttgacggt
     3601 aacgttgtaa aagctgctga attgctgggt gtcagccgtc caaccatcta tgatctgatg
     3661 agccggcacc agataaaata gccggtcttt taacttccct ggttcacaat aaatgacagt
     3721 tcttatccac gagcttattc aatgctcggc cagtcgtttt ccggattcgg aagcattggt
     3781 tcataaaaaa aaccggatca gctattcggc gatggcgcgg gaggtcgatc tcgcaaaaaa
     3841 ctcgattctt gacctggggc ttgactcagg ggagcgtgtg gctgtctacc tggagaaacg
     3901 tccggaaaca gtgattgcgc tattcggcgc cacggctgcg gggggagttt tcgttccagt
     3961 caatcccctc ctcaaggctg agcaggtcgc ttatattctt cgcgactgta atgtcaggat
     4021 tttggtcact tccgtcgaac ggttgcggct tctcgcgggg gtcttggaac actgccatga
     4081 tttaagaaca gtgatagtgg tcggcagaga agcccctcca gtcaccgttc gtcatgcaaa
     4141 cctgaagttc gtgcagtggg gagaggcttt ggcgcaagcc ggggcgatac gtgcgcatcc
     4201 ccgtatagac aacgacgtcg ccgcgatcct ttacacatcc ggaagcactg gcaaaccgaa
     4261 aggcgtcgtc ctttcccatc gcaacctcgt ggcaggggcg gccagcgtgt cggagtatct
     4321 gaaaaatcga tctgacgatc gcatcctgtc ggttttgccg ctgagctttg actacggtct
     4381 caatcagctt acgaccgcgt ttcatgtcgg cgccactgcc gttctgatga attacctgct
     4441 tccccgggac atcgttgacc tggtcaggga tgagaacatt acggggctgg ctgcggtgcc
     4501 tccgttatgg attcagctcg cccagctgaa atggccagac aacctgcctc tgcgttacat
     4561 cacgaattcc ggtggagcga tgccgcgcgc tacactggaa ctgttgcgca gccatcttcc
     4621 cggcacagct gtattcctga tgtatggcct caccgaggcg tttcgttcga cattcctgcc
     4681 cccggaacag gtgggcatac gaccagactc cattggcaag gcgattccca atgccgaagt
     4741 gctggtagtc cgggaagacg gttcgctttg tgcgccgggt gagccaggag aacttgtgca
     4801 tcggggcgtg catgtttcga tgggatattg gaacgatccg gaaaagaccg cggagcgttt
     4861 ccggcctgtt ccttcccgtc aaccaggact taccattcct gaactcgccg tatggtcagg
     4921 tgacacggtg cggatggacg aggagggatt cctgtatttc atagggcgcc gtgatgaaat
     4981 gatcaagacg tcgggttatc gcgtcagccc catggaagtg gaagaggtga tttacgccac
     5041 ggaactggtt gcggaggctg ccgcggtcgg agttcgccat cccgttctcg gtcaggcgat
     5101 tgccctgatt gcaacacccc gtgcaggccg ggtgcctgat gaacaggcgc ttttgtccgc
     5161 ctgcaagcac aatctccccg catacatgtt gcccagccgc gtcatataca aggaggggag
     5221 tttgccgcgc aacccgaacg gaaaaatcga ccggaaactc ctcgcggatg agctgcaaaa
     5281 cgtgttcatg gagaccgtga atgagtgata tgcgtccaaa acacgctgca atgcttcaat
     5341 ttccggttga ggacgactgt ctgcaagtgg ggggaatccc cctcacccga ctggctcaac
     5401 gcataggcag aacgccgttt tacgcatatg accgccggct tgtcagtgag cgcgtggcgt
     5461 ggttgcgtca ttgcctgcct gaagggattc acattcatta cgcgatgaag gcaaacccga
     5521 tgcccgcgct ggtccagcac atggctcacc tggtcgatgg aatagatgtt gcctctgccg
     5581 gcgagatgaa agtcgcgctc gacacgccat tggctgctga gaacatcagt ttcgccggac
     5641 ccggcaagcg tgatgatgag ctcgggtgcg caatcgctgc cggcatcgtg ataaaccttg
     5701 agtcggaaca ggaaatggag cgtgtgtccg cgctggcgga acgccagcat gtccggccga
     5761 aagttgcggt tcgtgtcaat ccgggttttg aactcaaatc ctcgggcatg aaaatgggag
     5821 gcggcgcaaa acagttcggc atagatgcag agcgtgttcc ggccgtgctt ggccgaatag
     5881 ggcagctcgg gcttgatttc gagggcttcc atatttttag cggttcccag aacctgaagg
     5941 tggcgtccat ccaggaagcg cacgaaaaga cctttgcgct tgccattgca ttggcgcagc
     6001 acgccccggg cgagattcgc ttggtaaata tcggaggcgg cttcgggata ccttatttcc
     6061 caggcgatga acccctggac ctgaaggccg tgggagacaa tctaaggcaa ttgctgcccg
     6121 aagcaaaacg ccggttacca gaagcacaag tggttgtcga gctcgggcga tacctggtgg
     6181 cagaagcggg tgtttatgtt tgccaggtac tggagcgaaa gaagtccagg gacaaaatat
     6241 ttctgattac ggatggggga ttgcatcatc atctggccgc atccggcaat ttcgggcagg
     6301 tcatccggaa gaattaccct gttattgttg gcaacaaggt gaatggggcc gcaagagaga
     6361 cagtttcggt agttggcccg ctttgtacgc cgctggacct tcttgccgac cagatggaga
     6421 tgggtcaggc cgaagcaggg gatttgatcg tcgtttttca atcgggggcg tatggcctga
     6481 ctgcaagtcc aactgcattc ctcagtcatc cggttcctgt cgaagtattg gtatagttga
     6541 gctggcctgt tccactcctt tgcccccggt gattccgtaa cggggaaaag gagccgatgt
     6601 tgcggcgttc tttggattgg ggtgttctag aggggtttaa attgaggaaa ttggcgtgag
     6661 tggattttgt ggctggatag gacagggaac ctcggttccc gagaaccttc aacttgtgcg
     6721 gcggatggca gaacccattt cacgtttcga cagcagcagt gtcaagtcca tatccggcaa
     6781 gcggagcgcg gcggcggtgg cggctccgga aggcagagct catctttatc aaaaagaagg
     6841 gcttactgtt gcaatcaggg gacggccgcg ttttatggat gaacgtcaag accctgttga
     6901 aacgtgtgaa ggtattgcaa aaagcgttgc cgagcagtgg cggaaccgac gtgcagaggt
     6961 ttttactggt ctgagcggtg cattttcgct ttgcatactg gatgaagatt ccaccgaagc
     7021 tgttctcgcg actgaccgga tgggaaccta tcccgtttcc ttccagttgt cggggcaagc
     7081 ccttgtcttc ggatcaaccg cggattcggt catcaggcat ccgtcaataa aacctgaaat
     7141 tgctccccaa agcctttaca actacgtcta tttccacatg atcccgggtc ccgacacgat
     7201 ttaccaggga cagaatcgtc ttctgcccgg ggagtatctg gtgtatcgca aaggccgtat
     7261 agagacccgc aaatattggg agatgcagtt cgttgaaagc gagacacggc cctttcagga
     7321 attgagggaa aattttctcg gtgtcctgag gtcgagtgta cgtactgcgg tggatggcga
     7381 ggaagtgggc gcttttctca gcggaggcac agacagttcc acgattgcgg gaatacttgg
     7441 ggaggttacg ggccagccgg ccagaaccta ttccataggg ttcgatgctt cgggttacga
     7501 cgagatgtcg tatgcccgca ttgctgcgag gcattttggc actcggcatc aggaatacta
     7561 cgtcacgcct gatgatatcg taacggctat tcctcaggtt gcaggtattt tcgaccaacc
     7621 tttcggtaac tcttccgcca ttccctcttt ctattgtgca cgcatggcga aagccgacgg
     7681 ccttgccagg atgctcggag gcgatggggg cgacgagctt tttgggggaa actcgcgcta
     7741 tgcaaaacag catctgttct cgctctatga aaggatacct gctctgttgc gcaaggcgct
     7801 catcgagccc atggttttcg gcattcccca gcgggtggcc ccacccatgc tgcgcaaggc
     7861 gcggagttac gtggaacagg catcgatcgg catgccagcg cggactgaaa cctataactt
     7921 gctggagcac tatggtgcag tagaagtttt tagccgggag ttccttgaaa ccgttgatcc
     7981 agcttatccc ggaatacttt taagcgagat ttatcatcag aacccagcag aaagcctgat
     8041 caaccgcatg ctggcatttg accggaaatt cacgcttgcg gacaacgacc tgcctaaagt
     8101 gagcaaggct tgcgagctgg ccgggatgga tattgcatac cctctcataa gcgacgagat
     8161 cgtcgatttt tccttgcggc tcgagcccca actcaagttg aaggggacca agttgcgtta
     8221 ctttttcaag gaggcactcc gcggttttct gccggatgag atcatcacga agcagaaaca
     8281 cggcttcggc ttaccctttg gggtgtggct tcaagaccac aagcccttgc agacactggc
     8341 ttcggacagc ctgagcgatc ttaaaacccg taatatcatc aaccgcgaat ttatcaacaa
     8401 gctgctcgga caacacctgg gcgagcatgc gggatatcac ggtacgatgg tctgggtgct
     8461 gatgatgctg gagcaatggt acagacagag agcggaaagc gtcaaagtct aggaagcaat
     8521 aaccgtttga gcgggcacgg cccgctttga cgtacttcct acaggtagat gctgccccgc
     8581 cctccccaat tgcattcttc ctgtcgcttt tcgcgcattc aatcccctca acccctttcg
     8641 tcaagcggct cccggattca tcaagatact cgcggtctgg gttttgcggg tacccctgtt
     8701 ggggcacatg ccaataaaat acttgcggaa tgggtgttag taatgaatcg gggaattggg
     8761 acagcagcgt tcggtgcgcg gcctacatgc ttatccaata cgaacgaacg gcgaccggca
     8821 agaagctgcg ggaaagaatg gaggcgcgcg tataggccga ccgactgttt aaatggtaga
     8881 gcaatatctg cggggctacg agactggctt gtatttggtg gatgaacttc ctgatacacc
     8941 atttcttatc gttgataggt aatcctcatg cgatgacctc tggagatatc gcgttaaaaa
     9001 cctcaaaatg atcatggttt ctcattatta tcagggatat g
//
LOCUS       DASSHJ010000169        11413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_80386, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000169 DASSHJ010000000
VERSION     DASSHJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11413
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80386"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1311)
                     /gene="rlmN"
                     /locus_tag="VFI43_06480"
     CDS             complement(205..1311)
                     /gene="rlmN"
                     /locus_tag="VFI43_06480"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7061809.1"
                     /translation="MSVNLLDFNATTLAGFCAEIGEKPFRAKQLLRWIHRFGESDFSL
                     MSELAKGLREKLAKTAVVEPPALLSDHGAADGTRKWLLSVGGGNGIETVFIPEATRGT
                     LCVSSQVGCALACAFCSTGRQGFNRNLTVAEIIGQLWWANKALAQQPNSEAASIRPEH
                     AITNVVMMGMGEPLANFENVVIALDLMLDDNAYGLSRRRVTVSTSGLVPAMDRLRERC
                     PVALAVSLHAPNDPLRDQLVPINRKYPISELLAACERYLEAAPRDFITFEYVMLDGIN
                     DSVAHAHELVRLTADIPCKFNLIPFNPFPNSGYKRSPRDAILRFRDVLMGAGLVTTVR
                     KTRGDDIDAACGQLAGKVLDRTRRSSRGLAGPAA"
     gene            complement(1349..1774)
                     /gene="ndk"
                     /locus_tag="VFI43_06485"
     CDS             complement(1349..1774)
                     /gene="ndk"
                     /locus_tag="VFI43_06485"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7061810.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGEIYSRFEAGGLKIIAAQMLRLSQ
                     ADAEGFYAVHKDRSFFSDLVKFMTSGPVMVQVLEGEDAIKKNRDLMGATDPKKAEKGT
                     IRADFAESIDANAVHGSDAPETAAVEIAYFFPSLRIYSR"
     gene            complement(2006..4108)
                     /locus_tag="VFI43_06490"
     CDS             complement(2006..4108)
                     /locus_tag="VFI43_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET7061811.1"
                     /translation="MSAWVNFTMLLGARSFPIGAVEAAKGFALSILTALTALAPVEAF
                     AQSLPPKAPEPPQIHYDITVRIDPAARRIEGRTIIIANTSEELALMLGRRFEVLNAQV
                     DDSSIGPAATVRNMRGWRIPGEKQLPRRIEMHWRGDLASLDTSLDHSQTLGRNEPASG
                     EAGTFLPDSSGWYPYLADKLASYRVTIDLPPGQRGLVPGRLIEESDSEKGYHARFEFP
                     APTGGIDLMAGPYKVESRSMRGAGGKTIQLRTYFHPQIADLAPSYLDAVKGYIDLYES
                     WIGPYPFTEFSVVSSPTPTGFGMPTLTYLGIEVLRLPFIRSTSLGHEVLHNWWGNGVY
                     PDYAHGNWSEGLTTFMADYTYKERESPEAARDMRLGWLRDFAALPPAQDSALATFTSR
                     THGASQIVGYHKAAMVFLMLRDWLGRDVFDRGLQSFWREQRFRIASWTDLRHAFETAS
                     GRDLHAFFDQWLQRPGAPAVQLTGAKYTDSDSGHLLAVTLEQGTPAYSVRVPVAIRTD
                     KGEESRTLDLQSSRQTFTLNMRDKPSEAALDPDFRIFRRLTPGEAPPILRQIMVDRTT
                     VTVLLPEGGEPREVAQTLAIKLQHREPNVVDSDRLPDAPAVVIGLQDQVDAWLARHKL
                     PGRPEVVDKKGSAQAWTLSRPNGMTLAVVSARDVASLAALIRPLPHYGRQSYVVFDGA
                     KVVGRGAWPTRPQVVKLP"
     gene            complement(4105..5415)
                     /locus_tag="VFI43_06495"
     CDS             complement(4105..5415)
                     /locus_tag="VFI43_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HET7061812.1"
                     /translation="MDADVQECPEMGRMSRDISIQEHDLKILPRVWNVFSFPRNLLVL
                     ACLLCVSPSVLWAQAKADSKEKAGVDCAPFGKWTVPGGGSISETEVIIRAARRRVVLL
                     GEAHDNPDHHRWQLQTLAALHAYHPDMVIGFEMFPRRVQGALDQWVAGELSEAQFLSA
                     SDWNTVWSTDANLYMPLFQFARMNRIPMIALNIDTRLRRSVAAKGLSGVPENEREGVT
                     PPAAPSNAYLDYLLPIYAEHERDGKKKGEALKDDRDFRRFVEAQQLWDRAMAQALHSA
                     LDRPGKPLVVGIMGSGHIKYGYGVPHQLKDLKVTEVGMLLPWNTGSSCEQLVAGTADA
                     VFGTATIAAAANEPPRQRLGIRFEMAPDGGARVLEVEKGSIAEAAGIRDSDLIIEAAG
                     VQIKRQDDIVGIVKRQTPGTWLPIKLKRGGETIELVAKFPAVHK"
     gene            complement(5373..9425)
                     /gene="purL"
                     /locus_tag="VFI43_06500"
                     /gene_synonym="purI"
     CDS             complement(5373..9425)
                     /gene="purL"
                     /locus_tag="VFI43_06500"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET7061813.1"
                     /translation="MLLQFRGRNALSPFRLEKLASVLATEVPQVSHIRAEHWYFCAVS
                     RELDHNESFILESLLDTGTGTEEGAASSDNGDVSPAAKLLLVLPRLGTISPWSTKATD
                     IAHHCGLGAVERLERGTAFYVQTGDEPFLELPSAAKGALLALIHDRMTEAVFSSFDEA
                     GLLFRHFAPAPLTTVDLLTGGRSALQKANDRMGLALSPEEIDYFASYFIESGRNPTDV
                     ELMMFAQANSEHCRHKIFNADWIIDGQPRELSLFSMIRNTHGQHPEGTVVAYADNSAI
                     IEGAEISRFYPGKDNEYGYADEETHILMKVETHNHPTAISPYPGAATGVGGEIRDEGA
                     TGRGAKPRAGLSGFSVSNLNIPGFIQPWEACPEPGRPSCYGKPGRIASALQIMLEGPI
                     GGASFNNEFGRPNLAGYFRTFEQFVAGEMRGYHKPIMLAGGIGQIASRDAFKETFPPG
                     TLLIQLGGPGMLIGLGGGAASSMDTGTNEERLDFDSVQRGNPEMERRAQEVIDFCWQI
                     KRRGLPNPILSIHDVGAGGLSNALPELLHGSHRGGTVNLRDIPSEEPGMTPMQIWSNE
                     AQERYVLAVDPESLPLFNSICERERCPYAVVGTATGNEKLVVSDPLFENRPVDMDLLV
                     LLGKPPKMIRNVSHIQRTLRPFAAAGLDLEDAAYRVLRLPTVGDKTFLITIGDRSIGG
                     MTARDQMVGPWQVPVADVAVTLMGFHTYLGEAFAVGERTPVALIDSAASARMAVGEAI
                     TNIAPASIRHIGEIKLSANWMAAAGHPGEDAALFDAVHAVGMEFCPRLGISIPVGKDS
                     MSMKTVWQTEPGHDGGQGGGQEKKQVIAPLSLIISAFSRVEDARKTLTPQLRTDCGAT
                     ELILIDLGEGKNRLGGSALAQVYQQTGDEAPDISASKGAEMLKAFFAAIQSLNNENKI
                     MAYHDRSDGGLFVTLCEMAFAGHVGVTINLDQLCFDPLANDTDGSELRPEALGGRFRE
                     RILSVLFNEELGAVIQVKVEHHAEVMEMLAKEGLRDQCFVIGGLNEKDEIRILRNNKA
                     IFAQKRVDLHRAWSETSYHMQKLRDHPECAQQEYDRLLDTEDPGLHVQIGFDVDADVS
                     APYIAKHIRPKIAILREQGVNGHVEMAAALDHAGFAAVDVHMSDIIAGRVSLNDYKGF
                     AACGGFSYGDVLGAGRGWAKSILFNRLARDEFEAFFQRADTFALGVCNGCQMMSWLHE
                     IIPGTEHWPRFVRNKSEQFEARFVMVEVPESPSLFFDGMAGSRMPVSVAHGEGRAEFN
                     GENEVEAGSSLVALRFVDNRGKSTELYPFNPSGSPRGIAGVTTPDGRFSILMPHPERG
                     FRTVQHSWHPGWREDAPWMRMFRNARKWVG"
     gene            9497..11062
                     /locus_tag="VFI43_06505"
     CDS             9497..11062
                     /locus_tag="VFI43_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7061814.1"
                     /translation="MTTLARNSTAVYTTAEIREIEHLAAASLTQSNLMEKAGLAAAEV
                     ARDELLDQDSVHVLVLAGPGNNGGDAFVVARHLREWGFKVTLVFVGERAGQPADAQNA
                     LDAWRVAGGEISSGIPSDIKWDAVVDGLFGIGLDQHGRDVAGKYLELVNEINRMNLPV
                     LAIDIPSGLGSDTGNVRGAAIRAALTVTFIGLKPGLLTNDGPDYCGALFLRDLDLDVP
                     SLKQPMSWMVDLGFAKELSPPPRPANSHKGMFGSIGIIGGASGMIGAALLAGTAALKL
                     GAGRVYLGLIAANAPSVDMVRPELMLRPVEDIFKLSQLNCLVVGPGLGTEPSVKQWLH
                     CALESDIPLVLDADALNLIAADPAISIKLRERIAPSILTPHPAEAARLLGTDVASVQK
                     DRMGSASELASKFNCCAVLKGAGSICAMPAGRRYINTSGNPGLSSAGTGDILSGIAGA
                     LLAQGLVGEKALVLAVYLHGAAADLLLDRIGGPVGMTASEIVGAARNLLNNWIYQDRA
                     PTARPVAEALRFD"
BASE COUNT         2458 a         3393 c         3108 g         2454 t
ORIGIN      
        1 ctcagtattg ggggcttcgg ctccggttga atcgttattg ctgctatgat cgggcgcttc
       61 catgacttga atggatcatt acctctacag ttctctgcaa ggtcggaaca acaattgaca
      121 agcgctcagg agtccacaga aataaatcgc cgtacgggca tacgtcaaaa cgaaaccaga
      181 aggcaaacga aaccggaatt gatattatgc tgctgggcca gccagcccgc ggctgctccg
      241 ccgagtcctg tcaagcactt ttcccgccaa ctgaccgcag gcagcgtcaa tatcatcgcc
      301 ccgtgtcttg cgcactgttg tgacgagccc tgcgcccatc agcacatcac gaaaacgcaa
      361 tattgcgtcc cgtggcgaac gcttataacc agaatttgga aaaggattga acggaatcag
      421 gttgaacttg caggggatat cggcagtcag ccgtaccagc tcatgcgcat gcgccacact
      481 atcgttgatg ccgtcaagca taacatattc gaacgtgatg aaatcccgcg gggccgcttc
      541 aaggtaacgc tcgcacgcgg cgagcagctc gctgatcggg tatttccggt tgatcggaac
      601 caattgatca cgcaacggat cgttcggcgc atgtaaagac accgccaggg cgacaggaca
      661 acgctcgcgc agccgatcca tggctggcac caatccggac gtgcttaccg taacacggcg
      721 gcgtgacaat ccgtacgcgt tgtcatccag catcaggtcg agcgcgatta caacgttttc
      781 gaaattggcg agcggctccc ccatgcccat catgaccaca ttcgtaatcg cgtgctccgg
      841 ccggattgaa gcagcttcgc tattcggctg ttgcgccaga gccttgtttg cccaccataa
      901 ctggccgatg atttccgcca ccgtcaggtt acggttgaag ccctgcctcc cggtggagca
      961 gaaagcgcag gcgagtgcac agccgacctg gctggaaacg cataatgtgc cgcgagtagc
     1021 ctccggtatg aagactgttt caataccgtt gccgccccct acggaaagca gccattttcg
     1081 agtgccatcc gccgccccat ggtctgagag cagggcaggt ggctcgacta cggcagtttt
     1141 cgcaagcttt tcgcgcagcc ctttcgccaa ctcgctcatc aaagagaaat ccgactcgcc
     1201 aaaccggtgt atccagcgca gcagttgctt ggcgcgaaac ggcttctcgc cgatttctgc
     1261 acagaaaccg gcgagtgttg tcgcattgaa atcgaggaga ttgacgctca tgtatgagcc
     1321 cgcaccgggc ttgaaggctt tatgggtttt agcgggaata aatgcgaagt gacgggaaga
     1381 aataggcaat ttctaccgcc gctgtttccg gggcgtcgga tccgtggaca gcgttagcat
     1441 caatgctctc ggcgaaatcc gcacgaatcg ttcctttttc agcctttttt ggatcagtgg
     1501 cgcccatcag gtcccggttc tttttaatcg cgtcttcacc ctcgagcacc tgtaccatga
     1561 cggggccgga agtcatgaat ttcaccaggt cgctaaagaa cgaacggtcc ttatgcacgg
     1621 cgtaaaaccc ttcggcatcc gcctgcgaaa gacgcagcat ttgcgctgca attattttta
     1681 aaccacccgc ctcaaagcgg gaataaattt ccccaatcac atttttcgcc accgcatccg
     1741 gttttatgat ggatagcgtt ctttccacag ccatgcaata ctccagaaaa agaaccaagt
     1801 ttctataaat taatgagtag tttaacaaaa aaccggtttt caggcccgaa aaaaagaaac
     1861 ccggcgtcag gccgggcttc actgtgatat atgagcggct gaattagttc cgaaaagtgt
     1921 cgccgtcccc tttacaattt tccctttaca tcccagagcg ctgggattgc ggacatctgc
     1981 ttcgcgatta ttcgatccat agatctcatg gcagcttcac aacttgggga cgcgtgggcc
     2041 acgcccctcg cccgactacc ttggctccat caaatacgac atagctttgt ctcccatagt
     2101 gtggcagcgg acgtatcaag gcagccagcg acgctacgtc acgcgcggaa accacggcaa
     2161 gcgtcattcc attcgggcgg ctcaacgtcc aggcctgcgc cgaacccttt ttatccacga
     2221 cctcaggccg tccgggcagt ttatgccggg ccagccaggc gtccacctgg tcctgcaatc
     2281 ctattaccac tgcgggcgca tcaggcaatc tgtccgagtc aaccacattg ggttcacgat
     2341 gttgcaattt tattgccagc gtttgcgcga cctcgcgcgg ctcgccgcct tcaggaagca
     2401 gcacggtcac agtcgtgcga tccaccatga tctggcgcaa aatgggcggc gcctctcccg
     2461 gcgtcaaccg gcggaaaatt cggaaatccg ggtcgagcgc agcttccgac ggcttgtcgc
     2521 gcatgttgag cgtgaatgtc tgtcgcgagc tttgcagatc cagcgtgcgg gattcttcgc
     2581 ccttatcggt gcgaatggca acgggcacgc gcacgctgta cgccggagtg ccctgctcca
     2641 gggtgacagc cagtaaatgc ccggaatccg aatccgtgta ctttgctccc gtcagctgta
     2701 ccgccggcgc tcccggccgc tggagccact gatcgaagaa agcatgtaaa tcgcgccctg
     2761 aggcagtctc gaacgcatgc ctcagatccg tccaggaagc tatccggaag cgctgctcgc
     2821 gccagaagga ctggagcccc cgatcgaaca catcccgtcc aagccaatcg cgcagcatca
     2881 gaaataccat tgccgccttg tgatacccca cgatctggga tgcgccatgc gtccgggaag
     2941 taaaagttgc gagcgccgaa tcctgcgccg gcggcaatgc cgcaaaatca cgcaaccagc
     3001 cgagccgcat atcccgggcc gcctccgggc tttcccgctc cttgtaggtg taatccgcca
     3061 tgaaagtcgt gagcccttcc gaccagttgc catgcgcgta atcggggtag acgccattgc
     3121 cccaccaatt gtgaagcact tcatgaccaa gagacgtcga tcgtatgaat ggcaggcgca
     3181 gtacctcgat tccgaggtag gtgagcgttg gcattccaaa gcccgtggga gtcgggctgg
     3241 atacgacact gaattcggta aaggggtaag ggccgatcca ggactcgtag agatcgatat
     3301 aaccctttac cgcatcgagg taggatggcg caaggtcggc gatctgcgga tggaaataag
     3361 tgcgcagctg gattgttttg ccacctgctc ctcgcatcga ccgggattcc actttgtaag
     3421 ggcccgccat cagatcaata ccgccggtcg gggccggaaa ctcgaagcgt gcgtggtatc
     3481 ccttctcgga gtctgattct tcaatcaggc ggcccggcac gagcccccgc tgccctgggg
     3541 ggagatcgat ggtgacccgg tagctcgcaa gcttatcagc gagataggga taccatccgc
     3601 tcgagtccgg caggaaagtg cccgcttcac cgctggcggg ttcattcctc cccaatgtct
     3661 gcgagtggtc gagggaagtg tccagggatg ccagatcgcc tcgccaatgc atctcgatac
     3721 ggcggggcaa ttgcttctcg ccggggatgc gccatccacg catattgcgg acagttgctg
     3781 ccggcccgat cgaggagtca tcaacctgag cattcaggac ctcgaaccgg cggccaagca
     3841 tcaacgcaag ctcctcggac gtgttcgcga tgataatggt acgtccttct attcttcgtg
     3901 ccgcagggtc gatccggacc gtaatgtcat agtgaatctg gggcggctcc ggcgccttag
     3961 gaggcaagga ttgcgcaaat gcctcaaccg gagcaagcgc tgtgagagcc gtgagaattg
     4021 agagcgcaaa acccttggca gcttccaccg ccccaatggg aaaagaacgg gcgccaagca
     4081 acattgtgaa atttacccac gcactcattt gtgcaccgcc gggaacttgg caacaagctc
     4141 tatcgtttcc ccgccgcgct tgagcttgat cggcaaccac gtaccagggg tctgtcgctt
     4201 cacgataccg acgatgtcat cctggcgctt gatctgcacg cccgcggctt cgatgatcag
     4261 gtcgctgtcg cgaatgcccg ctgcctcggc aatgctcccc ttctctacct ccaatacgcg
     4321 cgcgccgcca tccggcgcca tttcaaaccg aataccgagt cgttgccgcg gaggctcgtt
     4381 cgcagccgca gctatcgtgg ccgtgccgaa cacagcatcc gctgtgccgg ccaccagttg
     4441 ttcgcacgaa ctgccggtgt tccaaggtag taacatgccc acctcggtta ccttgagatc
     4501 ctttaattga tggggcactc cgtagccata tttgatatgg cccgacccca tgattcccac
     4561 gacgaggggc ttcccgggcc gatctagcgc ggaatggagg gcctgcgcca tggcgcgatc
     4621 ccaaagctgc tgggcctcca cgaaacggcg aaaatcccgg tcatccttga gtgcttctcc
     4681 tttctttttg ccgtcgcgtt cgtgctcggc atagatcggc agcagataat caagataggc
     4741 attgcttggt gcagcaggtg gcgtaacgcc ctctctctcg ttttcaggaa ctccgctgag
     4801 tccctttgcg gcgactgagc gcctcaagcg cgtatcgatg ttgagggcta tcattgggat
     4861 ccggttcatg cgcgcaaact ggaatagtgg catgtagagg ttcgcgtccg tactccagac
     4921 cgtattccag tccgatgcag agagaaactg ggcctctgac aactctcccg cgacccactg
     4981 atcaagcgct ccctgaaccc tgcgtggaaa catttcaaaa ccgattacca tatcagggtg
     5041 atacgcgtgc aacgcggcca gcgtctgcaa ttgccagcga tggtgatcgg gattgtcgtg
     5101 cgcttctccc aaaagcacca ctcgacgcct ggccgcccgg atgatgacct ctgtttcact
     5161 tatgctgccc ccgccgggca cggtccattt cccgaatggc gcgcaatcca cgcctgcctt
     5221 ttccttgctg tccgccttgg cctgggccca aaggactgac ggggaaacac ataacaggca
     5281 tgcaaggacc agcagatttc gtgggaaaga gaatacgttc cacactctcg gtaaaatttt
     5341 caagtcatgc tcctggattg atatgtcccg gctcatccta cccatttccg ggcattcctg
     5401 aacatccgca tccatggcgc gtcttcccgc caaccgggat gccatgagtg ctgcactgtg
     5461 cggaatccgc gctctgggtg aggcattaaa atactgaagc ggccatccgg cgttgtcacg
     5521 ccggcaatcc ctcggggtga cccgctggga ttaaaggggt agagttcggt cgacttgccg
     5581 cgattatcta cgaaacgcag cgcaaccagc gaactacccg cttcaacttc attctcgcca
     5641 ttgaactcgg cccggccttc gccatgcgca acggacaccg gcatccggct cccagccatc
     5701 ccgtcgaaaa acagggaggg gctctctggc acctccacca tgacgaagcg agcctcgaat
     5761 tgctccgatt tgtttcgtac gaaacgcggc cagtgttcag ttccgggaat gatctcatgc
     5821 aaccagctca tcatctggca tccgttgcat accccgaggg caaatgtgtc ggcgcgctgg
     5881 aagaaggcct cgaactcgtc ccttgcaagc cggttgaaaa gaatcgactt ggcccatccc
     5941 ctgcccgctc caagcacgtc accataggaa aacccgccgc atgccgcgaa tcccttgtag
     6001 tcgttcagcg aaacacgacc tgcgataatg tcgctcatat gcacatcgac cgcggcaaat
     6061 ccggcatgat ccaaggctgc cgccatctcg acatgcccgt tcacgccctg ttcgcgcaat
     6121 atggcgattt tcggacgaat gtgcttggca atgtaaggcg cggaaacatc tgcatccacg
     6181 tcaaagccga tttgtacatg caggcccgga tcttcggtat ccagcaggcg atcgtattcc
     6241 tgttgagcgc attccgggtg gtcgcgcagc ttctgcatgt gataactcgt ttcggaccac
     6301 gcccggtgca agtcaacccg cttctgggcg aatatcgcct tattgttgcg caggatgcgg
     6361 atctcatctt tttcattaag accaccgatg acgaaacatt ggtcgcgcag gccttccttc
     6421 gccagcattt ccatgacttc ggcatggtgt tccaccttta cctggattac ggcccccaat
     6481 tcctcgttga aaagaacgga aaggatacgc tccctgaaac ggccgcccag cgcttcaggc
     6541 ctcaattcac tcccgtctgt gtcgttggcc aatggatcga aacacaactg gtcgaggttg
     6601 atggtaacgc caacgtggcc cgcaaaagcc atttcgcata acgtcacgaa caatccgcca
     6661 tcggagcggt cgtggtacgc catgatttta ttttcgttat tcaacgactg tatggcggcg
     6721 aagaaagcct ttagcatttc cgctcctttc gacgcggaaa tatccggcgc ttcgtcgccc
     6781 gtttgctggt agacctgggc cagcgccgat ccacccaggc ggtttttgcc ttctccgagg
     6841 tcaatcagga taagctccgt cgccccacaa tccgtgcgca attgcggcgt gagtgtcttg
     6901 cgggcatcct ccactcttga aaaagcgctg ataatcaagg aaagcggcgc aatcacctgt
     6961 tttttttcct gcccccctcc ttgcccccca tcatgtccgg gttcagtctg ccacacggtc
     7021 ttcatcgaca ttgaatcctt gccaaccggt atgctgattc ccagccgggg gcaaaattcc
     7081 atgcccaccg catgcactgc atcgaacagc gcggcatcct cgcccggatg ccccgccgca
     7141 gccatccagt ttgcagaaag cttgatttct ccaatatggc gaatcgatgc tggcgcgata
     7201 ttcgtgatgg cttccccgac ggccatgcgg gccgaggcgg cggaatcgat cagggcaaca
     7261 ggcgtacgct cgcctaccgc gaaagcctcg cccagataag tgtgaaaacc catcagtgtc
     7321 acggcaacgt cagcaacggg cacttgccaa ggtcccacca tctggtcgcg cgccgtcatg
     7381 ccgccaatgc tgcggtcccc aatcgtgatg agaaaggtct tgtcgccgac tgtcggcaac
     7441 cgtaggacac ggtaggccgc atcttcaagg tcgagtcctg ccgccgcgaa cggtcgcagg
     7501 gttctttgga tatgcgagac gttgcgaatc attttcggcg gcttgcccag caataccaaa
     7561 agatccatat cgaccgggcg gttttcaaag agcggatcac ttaccaccag cttctcattc
     7621 ccagtcgcag tgccaactac cgcgtaagga caacgttcgc gctcacagat cgagttgaat
     7681 aacggcagcg attcgggatc gacggcgagc acgtagcgct cctgcgcttc gttactccaa
     7741 atttgcatgg gcgtcatgcc gggctcttcc gatggtatat cgcgcaggtt aactgttccg
     7801 ccccgatgag aaccgtgaag caactcgggc agggcattgg atagtccccc tgcgccaaca
     7861 tcgtgtatgg aaagtatggg gttggggagg ccgcgccttt ttatttgcca gcagaaatcg
     7921 ataacttcct gcgcgcggcg ttccatctcg ggattgccgc gctgcacaga gtcaaaatca
     7981 agcctctcct cgttggtgcc ggtatccatg ctcgaagccg cgccgccacc caggccgatc
     8041 agcatgcctg gcccgcccag ctgtatcaag agcgttccgg gcggaaaagt ttccttgaac
     8101 gcatcccggg aggcgatctg ccctatcccg cccgcaagca tgatcggctt gtgatacccg
     8161 cgcatctccc ccgccacgaa ctgctcgaag gtccgaaaat accccgcgag gttgggccgc
     8221 ccgaactcat tgttgaacga cgcgccgcct atcggtccct cgagcatgat ttgcaaggct
     8281 gaagcaatac gtccaggctt tccataacaa gagggacggc cgggctccgg gcaagcttcc
     8341 catggttgaa taaacccggg aatattcagg ttggacactg aaaaaccgct gaggccagcg
     8401 cgcggtttgg cgcctcgccc agttgccccc tcatcgcgga tttcccctcc cactccagtg
     8461 gctgcgccgg gataaggcga aatggcggtg ggatgattat gcgtttcgac tttcatcaga
     8521 atatgcgtct cttcatccgc ataaccgtat tcattgtcct tcccgggata gaagcgcgag
     8581 atttccgccc cctcaatgat tgcggaattg tcggcgtaag caacgacagt gccttccggg
     8641 tgctgcccgt gcgtattgcg gatcatggaa aacagcgaga gctcgcgcgg ttgcccgtcg
     8701 atgatccagt cagcgttgaa aatcttgtgt cggcaatgct cggagttggc ctgcgcgaac
     8761 atcatcaatt ccacatcagt tggatttcgc ccgctctcga taaaatagct cgcaaaatag
     8821 tcgatttctt ctggagacaa ggcgagtccc atccggtcat tggctttttg aagggcgctt
     8881 cgtcctcccg tcagcaagtc caccgtagtg agcggagcgg gagcaaaatg cctgaacagc
     8941 aaccccgcct cgtcaaacga gctaaagacg gcctccgtca tgcgatcatg tatcaaggca
     9001 agcaatgccc cttttgcagc agagggtaat tccaggaaag gttcatctcc cgtttgaaca
     9061 taaaacgcgg ttccccgttc gagcctctcc acggctccaa gtccgcaatg gtgggcgata
     9121 tccgtagcct ttgtcgacca cggtgatatc gtgccgagtc gaggcagaac aagcagcagc
     9181 ttcgcggcgg gtgagacatc tccattatca ctgcttgccg ctccctcttc cgtaccggta
     9241 ccggtatcaa gcaggctttc gagaatgaag ctttcgttgt gatccagctc ccgggagacg
     9301 gcgcaaaaat accaatgctc ggcgcgaata tgggaaactt gtgggacttc ggttgcaagg
     9361 acacttgcca gtttttccag gcggaaggga gaaagagcgt tacgccctcg aaactggaga
     9421 agcatcggag attttcggaa aaatcggaaa aacataattt tacacgagaa gccgagccag
     9481 ccccagccag gaacccatga ctacactcgc aagaaattcc acagcggttt atacgactgc
     9541 cgagattcga gaaatcgaac atcttgctgc agcctccctc actcaatcca acttgatgga
     9601 aaaggcaggc ctggctgccg cagaagtcgc gcgggatgag ttgctcgatc aggatagcgt
     9661 ccatgtgctg gtgttggcag gccccggtaa taatggcggg gatgcgttcg tggtcgcaag
     9721 gcatctgcgc gagtggggat tcaaggttac gctggtgttc gtgggagagc gagccggcca
     9781 gcctgctgat gctcaaaatg ccttggacgc gtggagggtc gcgggcggcg agatcagctc
     9841 gggcatccct tccgatatca aatgggatgc cgtggtggat ggactattcg gcattggatt
     9901 ggaccagcac gggcgcgacg tggcaggcaa ataccttgaa cttgtcaacg agatcaatcg
     9961 gatgaacctg ccggtgcttg ccatcgacat tcccagtggg ctgggcagcg ataccggtaa
    10021 cgttcggggc gccgcaatca gggcggccct aacagtcacg tttatcggcc tcaagcccgg
    10081 cctgctcacc aatgacggcc ctgattactg tggagcactt ttcctgcgcg atctcgatct
    10141 ggatgtccct tcgctgaagc agcccatgtc atggatggtt gatcttggct tcgcaaaaga
    10201 gctttcaccc ccacctcgcc ccgcaaacag ccataaaggc atgttcggca gtattggcat
    10261 catcgggggt gcgagcggga tgattggagc ggcccttctt gcggggacgg cggctttaaa
    10321 actgggggct gggcgcgtct atctcggctt gattgccgcc aatgcaccca gcgttgatat
    10381 ggtacgaccg gaattgatgc tgcggcctgt cgaggatatc ttcaagctga gtcagctgaa
    10441 ctgcctggtc gtggggcccg ggcttggtac tgaaccaagc gtaaaacagt ggctccactg
    10501 cgcgctggaa tccgatatac cgctcgtgct cgatgccgac gccttgaatc tcatcgcggc
    10561 tgacccggca atatcgatca agctgcgcga acggatcgct ccttcgatat tgaccccgca
    10621 tccggctgag gcggctcggc tgcttggcac tgacgttgcg tccgtccaaa aagaccgcat
    10681 ggggtccgct tcggaactgg cgtccaaatt caattgctgc gccgtgctga aaggcgcagg
    10741 cagcatttgc gccatgcccg ctggcaggcg ctacatcaac acaagcggca atccgggttt
    10801 gagcagtgca ggaacgggag atatattgtc aggtatcgcc ggcgctttgc tggcgcaggg
    10861 attggttggg gaaaaagccc tggtactggc agtttatctg catggggccg cagccgactt
    10921 gcttctcgac aggataggcg gcccggtcgg catgactgct tccgaaatcg tcggtgcggc
    10981 acgaaatctt ctgaataact ggatatatca agatagagcg ccaaccgcga ggcctgtggc
    11041 tgaagccttg cgttttgatt gaatcccact caccccaagc ctcttcgagg tccccctgtg
    11101 cggttccgca cagaatcgcc ctctcgatct gggttaaaag tcctgattcg agagagaaat
    11161 cgcagtgagg aattcaggcg gagttgttga agctcctcgt tactcgcaaa tcgagttcat
    11221 gaaaggagcg cgactttcgc tcccctcggg aatcctcctt ttcctcatta attgccctaa
    11281 tttatcaatc acaggagaag tctcatgaca gtacttgtta cccatttcgc gcccgatttt
    11341 accaaaccag ccgttttgcc cgatggcacg ttcaacgata cattcaattt tcgcacggcg
    11401 atctcgggaa aat
//
LOCUS       DASSHJ010000170        21018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1390940,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000170 DASSHJ010000000
VERSION     DASSHJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 21018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21018
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390940"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2164
                     /locus_tag="VFI43_06510"
     CDS             <1..2164
                     /locus_tag="VFI43_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7061815.1"
                     /translation="NWNKLLAYPKPKLSAEEKAFLNGSVEQLCAMLDEWHITRDLHDL
                     PPQAWQFIKENGFFGMIIPKQYGGLGFSALAHSEVVMKISSRSGTAAVSIMVPNSLGP
                     AELLLQYGTEEQKNYYLPRLAKGLEIPCFALTGPEAGSDAGSIPDTGIVCYGEFNGDP
                     HVLGMRVTWEKRYITLGPIATLLGLAFKLRDPDGLLEGKEDLGITLALIPTRTPGVNI
                     GRRHFPLDAAFQNGPNSGKDVFIPMDWVIGGREGIGSGWRMLMNCLAAGRSISLPATG
                     TGGAKLATRVSGAYGRVRVQFKTPIGRFEGVEEVLARLGGNTYMMDAARVMTAGAVDM
                     GEKPSVISAIVKYHLTERGRQAINDAMDIQGGKGICMGPSNYLGRGYQQIPVSITVEG
                     ANILTRNMIIFGQGAIRCHPYILAEIQAANDVNIERGQVKFDEALAGHAVFTVGNGFR
                     ALLHGLSGASLASAPGSAGPETQVYYSQLTRFSAAFAFLADISMLVLGGSLKRKERLS
                     ARLGDILSMLYLCSATLKRFNDDGRPAADLPLLRWSLQDALFRMQEAFDGVLENFPVR
                     TFAKGLLRFVVFPLGKPFSPPSDDLGHKVATAVMTAGEARDRLTSGIYTPASPDEPLN
                     VLEQALQCAVVSEAVEAKLRAAVKTGQISAHADDKIAEALKQRVITQAELELIGKMKT
                     LRRRVISVDDFPPDFSNEPASAPIVVAQGDPALAQTEL"
     gene            2205..4061
                     /locus_tag="VFI43_06515"
     CDS             2205..4061
                     /locus_tag="VFI43_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7061816.1"
                     /translation="MQIEQHNLSDIISVEEAGTLDGLFRERARRSPGRIAYRDYDRSS
                     GTWRDLTWSQMDARIAQWQAALARENLPAGERVAIMLRNCPDWVTFEQAALRLGLVVV
                     PLYTADRPENAAYIVQHSGARVLLIEDPAQWQLFSEVREQLSNLVRVLTIQGGPDRSN
                     DGLVLPVHDWLSQGMHEQPAGKPGNQSEQARRGGKADPYEMATIIYTSGTSGRPKGVM
                     LTHHNILTNAYSCLNWVSIRKDDLLLSFLPLSHTFERTGGYYVPMMRGATVAYARSVH
                     QLQEDLLTIRPTILVSVPRIYERIHAGISAKLAEGPGLNRKLFDLAVEIGYSRFEYQQ
                     GREPKRVSHLLWPVLEKLVARKVMAKLGGRLRGAMSGGAALSSRISRVFIGLGLPILQ
                     GYGMTESSPVVSSNRFDDNLPSSVGRPIPGVEVKLGENDALLIRGPNVMLGYWKDSEA
                     TRAVIGEDGWLNSGDIARIDEQGRITITGRLKEILVMSTGEKVPPADMEAAILHDPLF
                     EQVMLIGDGRSYLSALVVLNSRNWESIAGQYDLDPDWRKLSGERKLEEILLQRIAYQI
                     REFPGYAKVYRVALAHEPWTIENHMLTPTLKLRRMAVLEHYKTEVERLYEGH"
     gene            4137..4550
                     /locus_tag="VFI43_06520"
     CDS             4137..4550
                     /locus_tag="VFI43_06520"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET7061817.1"
                     /translation="MESAAEQHVTLPDEDPILRLVPMPTDTNYAGDVFGGWIMAQVDI
                     AGSLPAIRRARGRVATVAVNSFVFKQPVFVGDVVSFYAKIIRVGRTSITVDVSVYALR
                     GLRQGGEEICIKVTEAVLTYVAVDEQRKPRVLPPE"
     gene            4579..7107
                     /locus_tag="VFI43_06525"
     CDS             4579..7107
                     /locus_tag="VFI43_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HET7061818.1"
                     /translation="MDNRLGVRKAAVLGAGVMGAQIAAHLVNANIETLLFELPAEQGD
                     PNANVSKAVENLKKLEPAPLSSPARATSIQPANYRENLELLKECDLVIEAIAERMDWK
                     TSLYQQIAPHLAEDATFASNTSGLSINQLAQALPENLRRRFCGIHFFNPPRYMHLVEL
                     IPCKESDSAMLDKLEAFLVTYLGKGVIRAKDTPNFIANRIGVFSILATMHHAQQFGLR
                     FDVVDALTGPLIGRPKSATFRTADVVGLDILTHVIQTMHNGLPDDPWHQYFAVPGWLQ
                     ALVNRGELGQKTRRGVYQKVGKEIRVLDVARQEYVPSIAEADPEIGEMLKRKNPADKL
                     AELHSNPNPQAQFLWAIFRDVFHYCAFHLQEIADNARDVDFAMRWGFGWNQGPFETWQ
                     AAGWSPIAQWISEDIASGKAMSSVPLPQWVTDLGKEDNAVVNEQVEGVHRSQGSYAPG
                     SNTFHPRSSLPVYRRQLFPDRLITEPVQYGETIFETDAVRMWHTKGDNAHDSIAILSF
                     KSKMHTIGSDVLDGVQQAIDEAERNWRALVIWQIEPPFSAGANLQKATERLKSGEPPS
                     AFSLLAKKLKKTAQSAVLKAARRLNLADALMAGKLAEVEAMVAQFQQTSQDLRYSMIP
                     TVAAMDGLALGGGCEFVMHCDRAVATLESYVGLVEAGVGLLPAGGGCKELALRAAQNA
                     INGDPFPLLKKYYQSVATAQLSKSAEQAKELGYLRHADPVVMNRFELLYVAKAQALAM
                     AEAGYRPPLEARNIPVAGSTGIATIKGMLVNMREGNFISEHDYLIGSKIAYVMCGGDL
                     TPGSLVDENWFLDLERDAFIELLATEKTQARIEHTLKTGKPLRN"
     gene            7329..8531
                     /locus_tag="VFI43_06530"
     CDS             7329..8531
                     /locus_tag="VFI43_06530"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET7061819.1"
                     /translation="MTTRQTQDAYIAVAVRTPVGKAPRGMFKTVRPDDMLAHVLKAAM
                     NECPELDPAAIDDVITGCAMPEAEQGMNVARIGLLLAGIPNNVPGMTVNRFCASGLQA
                     VALAADRIRLGNADIIIASGTESMSMVPMTGNKMSLDPAIFQHDENVGIAYGMGVTGE
                     KVAQRWHVSREAQDEFATVSHTRALKAIESGEFKEEIAPYTVEEHRPDLATHEVRDVS
                     TVRDTDEGPRPGTNTEVLSKLKPVFAANGSVTAGNSSQMSDGAGAVVVASEAALKRFN
                     LTPIGRFLGYSVAGVPPEIMGIGPVKAIPKVLQQVGLKQDDLQWIELNEAFAAQSLAV
                     INELGLDRAKVNPLGGAIALGHPLGATGAIRVATLLHGLRRHHQKYGMVTMCVGTGMG
                     AAGVFEAL"
     gene            complement(8570..8755)
                     /locus_tag="VFI43_06535"
     CDS             complement(8570..8755)
                     /locus_tag="VFI43_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HET7061820.1"
                     /translation="MSMYESDHTKFMRELFEKNPKLSEEQKKARAIWWDKKLDPEERK
                     RFKEAAVTQSGYVYFGK"
     gene            complement(8842..9654)
                     /locus_tag="VFI43_06540"
     CDS             complement(8842..9654)
                     /locus_tag="VFI43_06540"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET7061821.1"
                     /translation="MGQNTSRWGREARVYVSERNGVRSLHLGNNFVQSAMRLAFPNHL
                     ELAYTRCMMGFLLFHPHPENIIMVGLGGGSLAKFVHYRLTRAKTTVVEINEEVISAAR
                     NYFCFPEDDERLHTVIGDGADYIATHPGEADVLMVDGFDGGCQVPSLCTQDFYNNARG
                     ALRKNGVLVVNLLGREKGFQNYMRRMESSFSGNVTTLTAEPVGNVVAFAFRQNPAKRL
                     WNDLPSRAVDLEQRFALPFTEFAKKLNPPAITNSQLSRPEMVNNRSVSGREP"
     gene            9854..10369
                     /locus_tag="VFI43_06545"
     CDS             9854..10369
                     /locus_tag="VFI43_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061822.1"
                     /translation="MNKPIVAFLMSSCLLSACANLGLKEPTQAYGGKFEGNRSALSRC
                     VVDKLQSDSRWVIRGFQYETWAYPDIEATEVYGYSLNDVPGIYARTSPTNPDAVGIYP
                     GMPTGMPIPKTHVYRKNAGTPPDINPNYLFILTLKRTDPETVFATLSGKRYESNIVWE
                     KLKACADGSSA"
     gene            10578..12065
                     /locus_tag="VFI43_06550"
     CDS             10578..12065
                     /locus_tag="VFI43_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HET7061823.1"
                     /translation="MKGFWSKRVSMPVLCTAGFALALLGLPLAPAFAGDFEDGMQFIL
                     NKDYPKAVESFKKAAAQGNTDAQFNLGVLYSRGRGIKQDYEQAALWFRKAAEQGDVPA
                     QSMLGFLYLKGQGVKQDYQQAMFWYLRAAEKGYAIAQHNLGVMYAKGQGVAQDYKQAL
                     SWYRKAAEQGHAPAQAILGFMYLKGQGVEEDDKQAAAWYLKAAEQGYPDAQYALGVLY
                     AKGRGVIKSDEQAVAWYRKAAEQGNADAQFNLGLMYASGSGVPADDQQASSWFLKAAE
                     RGSAKAQFKLGVIYAKGRGVQQNDYDAVTWYRKAADQGYAPAEFNLGTLYATGRGVVQ
                     DDREAILWYSKAAEQGDPDAQYNLGVRYDTGRGVPKDEQQALSWYQKAADQGYARAQY
                     SLGVKYDNGLGVIQDHAQALSWYRKAAEQGNKGAQTNLGVLYYKGTGVKKDYVEADKW
                     FSIASAAGYEDAKENRELMEKLMTPVQVAEAHKLAEDWTKAHQKQ"
     gene            complement(12194..12367)
                     /locus_tag="VFI43_06555"
     CDS             complement(12194..12367)
                     /locus_tag="VFI43_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061824.1"
                     /translation="MKKLIKLIMALALVPMLSGCWLLVAGGAGAYGGYKMKEKGYTLQ
                     NPITKEKGTQQTK"
     gene            12570..13043
                     /locus_tag="VFI43_06560"
     CDS             12570..13043
                     /locus_tag="VFI43_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061825.1"
                     /translation="MSAGSVSIPWMKRLLVIALAALLTACQSADTRRVGGLAGSIAGA
                     IGGSYLGRYLGGGTVGAIATSVAGGVAGYYVGAGIGGYLGRDDQKKMANASQQAVYTG
                     EPQTFSNPDSGVKGRAEVIRPADVSQQPSGNCKIIRQTVVLKDGTTHTEDVKSCK"
     gene            complement(13184..13414)
                     /gene="chaB"
                     /locus_tag="VFI43_06565"
     CDS             complement(13184..13414)
                     /gene="chaB"
                     /locus_tag="VFI43_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HET7061826.1"
                     /translation="MPYKTINDLPDSVKHVLPKHAEEIYRAAFNSAWDEYKDPDERRG
                     HESREEAAHKVAWAAVKKEYEKVGDEWKRKKE"
     gene            13801..15687
                     /locus_tag="VFI43_06570"
     CDS             13801..15687
                     /locus_tag="VFI43_06570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET7061827.1"
                     /translation="MHSIYSPSVSVVIPTYDQMRFIVRALDSLQAQTLNDWEAIVIDD
                     GSRDGTAEAVSSYLGDVRIHYYQTPENRGLGHALNEGISKAKAPLIAYLPSDDVYYRD
                     HLQSLKALLEMNPEAVLVYSGVCHHYNRLANGQIEGYPLQLVQCMHRKTTARWVERPE
                     LESDDLERLYWSRLRLAGSFVGTNSITCEWVSHPMQRHKLMQEPEGGINPFRSHYRVQ
                     EPLRFHTSVGHAIDEESHYRTMRERPDTPYAKDGLKILLVGELAYNADRVLALEEQGH
                     KLYGLWMQNPHWYNTVGPLPFGHVQDLPRDNWREAIRKIQPDVIYALLNWQAVPFAHE
                     VLMATPGIPFTWHFKEGPFICLDKGTWSELIDLHQYCDGQIFSSPEMRDWFDTIVPGL
                     SLSKPTHVLDGDLPKRDWFDQPRTPLLSATDGEIHTVSPGRPIGLHPQDVAELAKHRI
                     HLHFYGEITHGQWLKWIEKTQGMAQGYLHLHPNVDQSRWTEEFSQYDAGWLHLFESGN
                     GGEMRRANWDDLNYPARLSTLAAAGLPMLQKANDGAIVATQTLTKQLGIGIFFDETEE
                     LAAQLRDSEQFHAIRERVWQQRHLFTFDHHVPELVDFFRSVIRSASSKPRGKIVSGMA
                     RQSA"
     gene            16159..16635
                     /locus_tag="VFI43_06575"
     CDS             16159..16635
                     /locus_tag="VFI43_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HET7061828.1"
                     /translation="MYSEIREIQEEIHKCGAKWTASETEFVKTVDPEQRVHRLGVVLD
                     QEALERIRRQPALDMPALIARFEQKMGWVPEGGEADHLDLVRKRLDLQLDTLFPPWFP
                     WFFNVDWRNRKGRNNVTPVKDQGGCGSGVAFGTTGTLESMLLMNIMLIWICPKPNC"
     gene            16632..17005
                     /locus_tag="VFI43_06580"
                     /pseudo
     CDS             16632..17005
                     /locus_tag="VFI43_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
     gene            17108..17533
                     /locus_tag="VFI43_06585"
     CDS             17108..17533
                     /locus_tag="VFI43_06585"
                     /inference="COORDINATES: protein motif:HMM:NF039257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tectonin domain-containing protein"
                     /protein_id="HET7061829.1"
                     /translation="MESPWKPVAGALKYISAASDGSVWGVNAGDEIFRWNGNSWDQVP
                     GALKQISVGNAATVWGVNSDDEIYRWNGAAWDQVSGALKHVAVANDGTVWGVNSGDKI
                     FHWNGNSWDQVPGALKQISVGAAKLVWGVNESDAIFRLR"
     gene            17927..18184
                     /locus_tag="VFI43_06590"
                     /pseudo
     CDS             17927..18184
                     /locus_tag="VFI43_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            18302..18436
                     /locus_tag="VFI43_06595"
     CDS             18302..18436
                     /locus_tag="VFI43_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061830.1"
                     /translation="MPLGTPYSVIIGPLHDFNLVNPDTGQWPHYFPNIYDYVECQERH
                     "
     gene            complement(18921..19019)
                     /locus_tag="VFI43_06600"
                     /pseudo
     CDS             complement(18921..19019)
                     /locus_tag="VFI43_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
     gene            19503..20105
                     /locus_tag="VFI43_06605"
     CDS             19503..20105
                     /locus_tag="VFI43_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7061831.1"
                     /translation="MPRVFAYCRVSTADQTTQNQSMEIKAAGFAIEARRLIEESISGS
                     VTAKERPGFMKLVDRMEPGDVLVVTKLDRLGRNAMDVRATVEQLASFGVRVHCLALGD
                     VDLTSPAGKMTMQVIAAVAEFERDLLIERTQSGIIRAKAAGKQFGRPPSLNMEERAAV
                     VERLDAGANIAEIAREFKTTRQTIMRVKEAALKLPSQQKE"
BASE COUNT         4969 a         5479 c         6031 g         4539 t
ORIGIN      
        1 aaactggaac aagctgctcg cttacccgaa gccgaagctg tccgccgaag aaaaggcttt
       61 tctcaacggt tcggttgaac agttatgcgc gatgctggac gaatggcata tcacccgtga
      121 tctgcacgac ctgccaccgc aggcatggca gttcattaag gagaacggtt ttttcgggat
      181 gatcatcccg aagcagtatg gaggcctcgg cttttcagcg cttgcgcatt ccgaagttgt
      241 catgaagatc agctcccgca gcggtactgc ggcagtatcg atcatggtgc ccaattcgct
      301 ggggcccgcc gaactgctgc tgcaatatgg tacggaagag cagaaaaact actatctgcc
      361 gcggctggcg aaaggattgg aaatcccttg ctttgcgctc accggcccgg aagcgggatc
      421 ggatgcgggg tccatcccgg ataccggcat cgtgtgctat ggagagttca acggcgatcc
      481 gcatgtgctg ggcatgcgcg tcacgtggga aaaacgctat atcacccttg ggcctatcgc
      541 aacgcttctt ggccttgcct tcaaactgcg cgaccccgac ggcctgctcg aaggcaagga
      601 agacctcggg attacgctgg cactgatacc cacccggaca cccggggtca atatcggccg
      661 acgtcacttc ccgctcgacg cagccttcca gaacggccca aactccggca aggatgtttt
      721 tatcccgatg gattgggtga tcggaggacg cgaggggata ggcagcggct ggcgcatgct
      781 aatgaactgc cttgcagccg gacgctcgat ttcgcttccc gctaccggta caggcggagc
      841 caagcttgca acacgggtga gcggtgcata tggaagagtg cgggtgcagt tcaagacccc
      901 tatcgggcgt tttgaaggcg tggaagaagt ccttgcccgt ctcggcggta acacgtacat
      961 gatggacgct gcccgcgtca tgacggccgg agcggtcgat atgggggaaa aaccttccgt
     1021 gatctctgca atcgtgaaat accatctgac cgaacgcggg cgccaggcga tcaatgacgc
     1081 catggatatt cagggaggca aaggcatctg catggggccc agcaattacc tcgggcgcgg
     1141 ctaccagcag attccggtga gcatcaccgt tgaaggcgcc aacattctga cccgcaacat
     1201 gatcattttc gggcagggcg caatacgctg ccacccttat atccttgcgg aaatacaggc
     1261 ggcaaatgat gtcaacatcg agcgcgggca ggtgaagttc gacgaggcac tcgcagggca
     1321 tgcggtcttt acggtcggga acggctttcg cgccctgctc catggcctga gcggcgcctc
     1381 cctcgccagc gctcccggca gcgctggccc ggaaacacag gtttactaca gtcagctgac
     1441 gaggttttcc gccgcattcg cctttctggc agatatctcc atgcttgttc tgggcgggtc
     1501 gttgaagcga aaggaaagac tgtcagcccg actgggtgat attctcagca tgctttacct
     1561 gtgctcggca acgctgaagc gtttcaatga cgacgggcgg cctgccgctg atctcccgct
     1621 gctccgttgg tcgctccagg atgcactctt tcgcatgcag gaagcctttg atggcgtgct
     1681 ggagaatttc ccggttcgga cgtttgcaaa gggcctcctg cgttttgtcg tctttcccct
     1741 gggaaaacca ttttcgccgc catcggacga tcttgggcat aaagtggcaa ctgcggtgat
     1801 gacggcagga gaagcgcgcg atcggctgac ttcgggcatt tacacacccg cgtccccgga
     1861 cgaaccgctg aatgtcctgg agcaagcgtt gcaatgcgcg gtggtcagcg aagccgtaga
     1921 ggcgaaactg cgggctgccg tgaaaacggg tcagataagc gcgcacgcgg atgacaaaat
     1981 cgccgaagcc ctcaaacaga gagtaatcac ccaggcagaa ctcgaactga ttggaaaaat
     2041 gaagacactg cgccgccgag tgatcagcgt ggatgacttt cctcctgatt tctcgaatga
     2101 acctgcttca gcaccaatcg ttgtggctca aggggaccct gcgctcgccc agacagagct
     2161 ttaaaaaaga caacgacaac agagaacaag aaggtacaaa ctccatgcaa atcgagcagc
     2221 ataacctctc ggacatcatt tcggtggagg aggccggaac gctcgatgga ttatttcggg
     2281 agcgcgcgcg gcgctcgccc gggcggatag cctaccgcga ttatgatcgc tccagcggta
     2341 cctggcggga ccttacctgg tcgcaaatgg atgcgcgtat tgcgcagtgg caggccgctc
     2401 tggcgcggga aaacttgccg gcaggggagc gcgtggcgat catgctcagg aactgcccgg
     2461 actgggtgac gttcgagcag gcagcgctga gactggggct cgtggtggtg ccgttatata
     2521 cggccgatcg cccggaaaac gcggcctaca ttgttcagca ttccggcgca agggtattgc
     2581 tgatcgaaga tccggcccag tggcaattgt tctctgaggt ccgagagcag ttaagcaacc
     2641 tggtgcgggt acttacgatc cagggtgggc ctgacaggag caacgacggg ctcgtgcttc
     2701 cagtgcatga ctggctctcg caaggaatgc atgagcagcc ggctggaaag ccgggaaatc
     2761 aatccgagca agcgaggcgg gggggaaagg ctgatccgta cgaaatggcg acgatcatat
     2821 atacgtcagg aacttccggc cgcccgaagg gagtaatgct gacccaccat aatatcctta
     2881 cgaatgcgta tagctgcctg aactgggtat cgatccggaa ggatgatctt ttgctttcat
     2941 ttctgccgct ctcccataca ttcgaacgca ccggcggata ctatgtaccg atgatgcgtg
     3001 gtgctacagt tgcctatgca cgttcggtgc accagttgca ggaggatctg ttgactattc
     3061 gccccactat cctggtttcc gtcccgagaa tctatgaacg aatccatgcc gggatcagcg
     3121 caaagcttgc ggaaggcccc ggcctgaacc gcaagctgtt cgacctcgca gtggaaatcg
     3181 ggtatagccg atttgaatat cagcagggaa gagaaccaaa acgcgtttcc cacctgttgt
     3241 ggcccgtgct ggagaaactc gtggcgcgga aggtcatggc caaactgggc gggcgcttaa
     3301 ggggggcgat gagcggcggg gcggcgttgt cgagccggat ttcccgcgta tttatcggtc
     3361 ttggattgcc tatcctgcaa ggctatggga tgaccgaaag cagtccagtg gtctccagca
     3421 acaggtttga cgacaatctt ccgagcagcg tgggacggcc tattccagga gtggaagtca
     3481 agcttggtga aaatgatgcc ctgctgatcc gcggaccaaa cgtgatgctg ggttactgga
     3541 aagactccga agcgactcgc gcagtgatag gagaggatgg atggcttaat tccggagata
     3601 tcgcccgcat tgacgaacag gggcgtatca ccataaccgg ccggttgaag gaaatccttg
     3661 tcatgtccac gggcgaaaag gttccgcccg cagatatgga agctgcaatc ctgcatgacc
     3721 cattattcga gcaggtcatg ctgatcggcg atggacgctc gtatctcagc gctcttgtgg
     3781 tgcttaactc gcgaaactgg gaaagcattg cgggccagta tgatcttgat ccggactggc
     3841 ggaagctatc aggcgagcgc aagcttgaag aaattcttct gcaaaggatt gcgtaccaga
     3901 taagagaatt tccggggtat gccaaggtat atcgggttgc gcttgcgcat gagccatgga
     3961 caatcgaaaa ccacatgctc acaccgacgc tgaagctacg acgtatggcc gtgctggagc
     4021 attacaaaac cgaggtggaa aggctttacg aaggacattg atgcgcccgc cccgattcgc
     4081 ctttcagtgg gaaggaagag gcggggtaag cagttgtgcg aagagcggga ggcaggatgg
     4141 aaagcgcagc ggagcaacac gtgacactgc cggatgagga tccgatattg cggctggtgc
     4201 cgatgccgac ggataccaat tatgcagggg atgtatttgg gggatggatc atggcacagg
     4261 tggatatcgc cggaagcctg cccgccatcc gccgcgcccg gggacgggtc gcgaccgtgg
     4321 cggtgaactc attcgtgttc aagcagccgg tattcgtggg cgatgtggtg agtttttatg
     4381 ccaaaatcat ccgtgtaggc cgcacgtcca ttaccgtgga tgtttctgtc tatgcactgc
     4441 gcggtcttcg ccagggcggc gaggaaatat gcatcaaggt taccgaggcc gtgcttacct
     4501 atgttgccgt tgacgagcag cgcaaaccac gcgtacttcc acccgagtaa aaacgaggtg
     4561 gaccatacag gaggcaaaat ggacaaccga ctcggagtgc gaaaagccgc agtgcttggt
     4621 gcgggggtaa tgggggctca gatagcggct cacctggtta acgccaatat cgaaacacta
     4681 ctgttcgaac tgccagcgga gcagggtgat cccaatgcca acgtaagcaa agctgtcgaa
     4741 aatctgaaga agctggagcc cgcgccactc tcgagccctg ccagggctac atccatacag
     4801 ccggcaaact accgggaaaa cctggaattg ctcaaggaat gcgaccttgt catcgaagca
     4861 attgccgagc gcatggactg gaaaacgagc ctgtatcagc aaatagcccc gcatcttgca
     4921 gaggacgcca ctttcgccag caacacatcc ggcctctcca tcaatcaact cgcccaggct
     4981 ttgcccgaaa atctgcgccg tcgcttttgc gggattcact ttttcaatcc gccgcgatac
     5041 atgcacctcg tggagctgat cccgtgcaag gaaagcgact ccgcgatgct cgacaagctc
     5101 gaagcgtttt tggttactta tctgggcaaa ggcgtgatac gtgcgaagga cacgccgaat
     5161 ttcatcgcca atcgaatcgg cgtgttttcc attctcgcca ccatgcatca tgcacaacag
     5221 tttggcctca ggtttgatgt cgtggacgca ctgacgggtc cgttgatcgg gcgacccaaa
     5281 agcgccacgt tccgcacggc tgatgtggtg ggactcgata ttctcacaca cgtgatccag
     5341 acgatgcata acgggctccc ggacgatcct tggcatcaat attttgccgt gccgggctgg
     5401 ctgcaagcgt tggtgaatcg cggggagctc gggcaaaaaa ccaggcgcgg cgtctatcaa
     5461 aaagtgggta aggaaatccg tgtgctggat gttgcgcgcc aggaatatgt cccctcgatc
     5521 gccgaggccg accccgaaat cggggaaatg ctcaagcgca aaaacccggc ggacaagctt
     5581 gcggagttgc attcgaaccc gaatcctcaa gcgcagtttc tatgggcgat tttccgcgac
     5641 gtgttccact attgcgcgtt ccacctgcag gaaatcgccg acaatgcccg cgacgtggat
     5701 tttgccatgc gctgggggtt tggctggaat caggggccct ttgaaacctg gcaggcggca
     5761 ggatggagcc ccattgccca gtggataagc gaagatattg cgtctggcaa ggccatgagc
     5821 tcagtcccgc ttccgcaatg ggttaccgac ctcggcaagg aagacaatgc agtggttaat
     5881 gagcaggtag agggggtcca tcgttcgcag ggttcttatg cacccggctc caataccttt
     5941 cacccgcggt cgtccttgcc cgtttatcgg cgccagttgt ttcccgaccg gttgataacc
     6001 gagcccgtcc agtatggcga gacgatattt gaaaccgacg cggtgaggat gtggcatacc
     6061 aaaggtgata acgcgcacga cagcattgcc atcctcagct ttaaaagcaa gatgcatacg
     6121 atcggcagtg atgtgctgga tggtgtccag caggctatcg acgaagccga gcgtaactgg
     6181 cgcgccctag tgatctggca gatagagccg ccgttctccg ctggcgcgaa tttgcagaaa
     6241 gcaacagagc ggctaaaaag cggcgagccg ccttcagcct tcagcctgct tgccaaaaag
     6301 ctcaaaaaga cagcacagtc agcagtgcta aaggcagcgc ggcgcctcaa cctggcagat
     6361 gcactgatgg cgggaaagct ggccgaggtg gaagcgatgg tggcgcagtt ccagcagact
     6421 tcgcaggatc tcaggtattc gatgattccc actgtggctg ccatggacgg actggccctg
     6481 gggggcgggt gcgaattcgt gatgcactgc gaccgcgcgg tcgccacgct ggaaagctat
     6541 gtggggctcg tcgaagcggg ggtgggtctt ttgccggccg gtgggggttg caaggaactt
     6601 gccttaaggg cggcacagaa tgcaataaac ggggatcctt ttccgttgct gaaaaaatac
     6661 taccaaagcg tcgccacggc gcagctgtca aaaagcgcgg agcaggcgaa ggaactggga
     6721 taccttcgcc atgcggaccc ggtcgtgatg aaccggttcg agctcctgta tgtcgccaag
     6781 gcacaggcgc tggcgatggc tgaagccggc taccggcctc ccctcgaggc acgcaacatt
     6841 cctgtcgcgg gatcgaccgg gatagcgact atcaagggaa tgctggttaa catgcgggaa
     6901 ggcaacttta tttcagaaca tgactacctc atcggcagca agatcgcgta cgtgatgtgt
     6961 ggcggcgacc tcacgcctgg cagcctggtg gacgagaact ggttcctcga tctggagaga
     7021 gatgcattta tagaattgct ggcgacggaa aaaacacaag cgcgtattga gcatacgctg
     7081 aagaccggca agccgttgag gaactaggta acgacaagcg acggggcgtg cccggatagc
     7141 atgtctgtga accgtaacca actcgcttac gtctaatttg taatagaaag gagaacaagg
     7201 cggcgaggag gaggcgacga cgacagtaca aatagtacac aaattgtaag cccgggacgc
     7261 cgaagacggg tcaacgcagc tatcgtttcc acagtttcgc tctatacgaa ttagcgttaa
     7321 aggaaaacat gacaaccaga caaactcaag atgcctacat cgccgtcgca gtgcggactc
     7381 cagtggggaa ggcgccgcgg ggaatgttta agacagtccg gccagacgac atgctggctc
     7441 acgtgctgaa agcggcaatg aacgaatgcc cggagcttga cccggcagcc atagacgacg
     7501 taatcaccgg ctgcgccatg ccggaagccg agcagggaat gaatgtagcc cgcatcgggc
     7561 ttttgctcgc cggcattccc aacaatgtcc ccggaatgac ggtcaaccgt ttctgcgcat
     7621 ccggcctcca ggccgtggcg cttgctgctg atcgcatccg cctgggcaat gccgacataa
     7681 ttattgcctc tggaaccgag agcatgagca tggtccccat gacagggaac aagatgtcgc
     7741 ttgacccggc catatttcaa cacgacgaga atgtaggtat tgcctacggc atgggcgtga
     7801 cgggggaaaa agtggcgcaa cggtggcacg taagccgcga agcacaggat gaattcgcaa
     7861 ccgtgagcca caccagggcg cttaaggcta tcgaaagcgg agaattcaag gaagaaatcg
     7921 cgccctatac agtggaagag cacaggcccg atcttgcaac tcacgaagtc cgtgatgttt
     7981 ccacagtcag ggatactgac gaaggaccgc gtcccggcac gaatacagag gtgctttcaa
     8041 aactcaaacc ggtatttgcg gcaaatgggt cggttaccgc tggcaacagt tcccagatgt
     8101 ccgacggggc tggcgcggtc gtggtcgcga gcgaagcagc actcaagcgc ttcaatctta
     8161 ctcccattgg ccgttttctc ggatattcgg tcgcgggcgt tccgccggaa atcatgggaa
     8221 tcggaccggt caaggccata cccaaggttt tgcagcaggt tgggttaaag caggacgacc
     8281 tgcaatggat tgagctgaat gaagcttttg ccgcccagag cctggcggtt atcaacgaac
     8341 tcgggctgga tcgcgcgaag gtcaacccgc tggggggcgc tatcgcactc gggcatcccc
     8401 tgggggcaac cggcgcgatc cgcgtagcca cgctgctgca tggcctgcgt cggcaccatc
     8461 agaaatacgg gatggtgacg atgtgcgtgg ggacgggaat gggcgctgcg ggtgttttcg
     8521 aagctctgta atgtcgccaa ggcagaatag cgttgacgtt cagtgtccct tattttccga
     8581 aatagacgta tccgctctgg gttaccgccg cttccttgaa gcgtttgcgc tcctcaggat
     8641 caagcttctt gtcccaccag atggcgcggg cttttttctg ttcttcagac agcttggggt
     8701 ttttttcgaa taattcccgc ataaatttgg tgtggtcgga ttcgtacata ctcatgtgtg
     8761 atttcttcct tatcaagagc ttgagttttt gaactttacc atatttgcgg gttccagaca
     8821 gctcactacg ttgccagcgc tctatggctc cctgcctgaa accgatctgt tattgaccat
     8881 ctccgggcgg gaaagttgag aattcgtgat tgcgggcgga tttaattttt tagcgaattc
     8941 agtgaatggc aacgcaaatc gctgctcgag gtccacggct cgactgggca agtcattcca
     9001 taaacgcttt gcgggattct gccggaaagc gaacgccacc acgtttccga ccggctcggc
     9061 tgtcagagtt gtcacatttc ccgaaaaact gctctccatg cgcctcatgt aattctggaa
     9121 tcccttttct ctcccgagca ggttaaccac cagtactccg ttttttctta atgccccgcg
     9181 cgcgttgtta taaaaatcct gggtacagag cgaggggacc tggcagcccc catcgaaccc
     9241 atccaccatg agcacatccg cctcgcccgg atgggtggcg atgtaatcgg caccgtcgcc
     9301 tattaccgta tgcaaccgct cgtcgtcctc aggaaaacag aaataattgc gggcggcgga
     9361 tataacctcc tcgtttatct cgaccactgt tgttttagcc cgcgtgagcc ggtaatggac
     9421 gaacttcgcc agggagcccc cgccaaggcc aaccattatg atgttttcgg gatgtggatg
     9481 aaaaagaagg aagcccatca tgcagcgcgt ataagcaagt tccagatgat tggggaaagc
     9541 cagcctcatc gcgctttgca cgaagttatt gccgagatga agcgaacgaa cgccattgcg
     9601 ctcgctcacg taaacccgtg cttcccttcc ccaacgcgat gtgttctgac ccattttgct
     9661 ctacgcgcga taatcccggc acctgcttta caaaaaagtc tgtttggcct atttttcgag
     9721 acaaaatggc aataaatcgg tcaaaaactc catgtaaaca gacttgtgcc gggccattct
     9781 cttcgctcca ggcgcataat gaccacgtct gattcaattc tactgatcta gcacctctga
     9841 gctcgatata cttatgaaca agccaattgt agccttcctt atgtcgtcct gcttgttgtc
     9901 ggcctgcgcc aacttgggac tcaaagagcc gactcaagca tacggaggca aattcgaggg
     9961 aaatcgttcg gcactctcgc gttgcgttgt cgacaaactg cagtcggaca gccgctgggt
    10021 gattagggga tttcaatacg agacctgggc ttacccggac attgaagcga ccgaggttta
    10081 tggttattca ctgaacgacg taccaggtat ctatgcgcgg acctccccca ctaaccccga
    10141 cgcagtgggc atttatcctg gaatgccaac cgggatgccg attccgaaga cgcatgtcta
    10201 caggaaaaat gctggaacgc ctcccgacat taatccgaat tatctgttca tcctgacctt
    10261 gaagcgaacc gaccccgaga cagtgtttgc gacgctgagc ggaaagcgtt acgagagtaa
    10321 tatcgtatgg gaaaagctga aggcatgcgc ggatggttca tcggcttgaa ccagctttat
    10381 ccgacgacct atgtggcgtc gtcctgtttg ccattctgtt aaatgccgga gccccgatcg
    10441 tgaatggcgg aaaatagcga gactcccatg ttcggtgtga tagcggatgt cctccgggga
    10501 taaaatggcg atctcggcaa aaagttgcga ttcaaattgg aattcaagtg acaagtgcag
    10561 gcgacaatgg tgagtgtatg aaggggtttt ggtccaaaag agtgtcgatg ccagtgctat
    10621 gcaccgcggg gtttgcactt gcgttgctcg gtctgccact agcccccgcc ttcgcgggag
    10681 atttcgagga tggaatgcaa ttcatcctca acaaggatta tccgaaagca gtcgaatcct
    10741 tcaagaaagc ggcagcgcag ggtaataccg atgcgcagtt caacctgggt gttttatatt
    10801 cgcgcggacg cgggatcaaa caagattatg agcaggctgc cctatggttc cgcaaggcgg
    10861 ctgagcaagg ggacgtcccg gcgcaatcca tgctcggctt cctctatctc aaggggcagg
    10921 gcgtcaagca ggattatcag caggcgatgt tctggtatct tcgcgcggcg gaaaaaggct
    10981 acgcgatcgc ccagcacaac ctgggggtca tgtatgccaa gggacaaggg gttgcgcagg
    11041 attataaaca ggcactatcc tggtaccgca aggcggcgga acaggggcac gcgcccgcgc
    11101 aggccatcct gggcttcatg tatctcaaag gccagggggt cgaggaggac gacaagcaag
    11161 cggctgcctg gtatctgaaa gcggcggaac agggataccc cgacgcgcaa tacgcgcttg
    11221 gcgtactcta tgccaaaggc cgtggcgtga taaaaagtga tgagcaggcg gtcgcatggt
    11281 atcgcaaggc cgccgaacag ggaaatgccg acgcccagtt caaccttggg ctgatgtacg
    11341 cttctggcag cggcgtaccc gcagacgatc agcaggcctc gtcctggttt ctcaaggccg
    11401 cagaacgtgg cagcgcgaaa gctcaattca agctgggcgt gatatatgcc aaaggccggg
    11461 gggttcagca gaacgactat gacgcggtca cgtggtaccg gaaagccgcc gatcagggtt
    11521 atgcgccggc ggaattcaat ctggggacgc tgtatgccac cggccgggga gtcgtacagg
    11581 acgatcgcga agcgatatta tggtatagca aggccgcgga gcaaggggat ccggacgccc
    11641 aatacaatct cggcgtaagg tatgacacgg ggcggggagt gccgaaggat gagcagcagg
    11701 cgctatcctg gtaccagaaa gcggccgatc agggctacgc gcgcgctcaa tatagcttgg
    11761 gggtaaagta tgataatggg ctgggggtca tccaggacca cgcccaggcc ctttcgtggt
    11821 atcggaaagc agcggagcaa ggcaacaagg gcgcacaaac gaaccttggc gtactgtatt
    11881 acaagggcac tggcgtcaaa aaggattatg tcgaggcaga caagtggttc agcatcgcca
    11941 gcgccgcggg ttatgaggat gccaaggaga accgcgaact tatggaaaaa ctcatgacgc
    12001 cggtacaggt ggcggaagcg cacaagctgg cggaggactg gacaaaagcg caccagaagc
    12061 agtaaaaaac tggaaaagcg ggtcctgctt acgcttgcaa gcactatcag ccactgttag
    12121 tcactgtaaa aactgcccgc cgctcccgca tgcttcctta tttgtcccgc gcaggagcct
    12181 tgatttgagc aggttacttg gtctgttggg tgcccttctc cttcgtaatc gggttctgga
    12241 gtgtatagcc cttttccttc atcttgtatc caccataggc cccggctcct ccggcaacaa
    12301 gcaaccagca gcccgaaagc atgggtacca gcgcgagggc cataatcagc ttaatgagct
    12361 tcttcatctt tcctgttttc tgtccataaa aaccacatgc cttatttcag tgcgcaaaaa
    12421 ttacaagccg atgccaacac gtttgaaccg tggttggaag ctttgctaat gataactcag
    12481 tgggttagcg gcgggaatcc gcagcgatca cgaagagtgg ccgaaaataa tgtagcggct
    12541 gggctagaat ataactttgg ggagcgatca tgagtgcagg aagtgtttcc attccatgga
    12601 tgaagaggct gctggtcatt gcgcttgccg cgctcctgac ggcatgccaa tcggccgata
    12661 ctaggcgggt cggcggactg gccggaagta tcgctggtgc gatcggagga agctatcttg
    12721 gaagatacct gggaggcggc actgtaggag ccatcgccac ctcggttgca ggtggcgttg
    12781 caggatacta cgtcggagcg gggattggag gctatctggg ccgggacgac cagaaaaaaa
    12841 tggccaacgc ttcacagcaa gcggtctata cgggagaacc ccagacgttc agcaatcctg
    12901 atagcggagt gaagggccgc gcggaggtca tacggcccgc cgatgtgtct cagcaaccct
    12961 cgggtaactg caagatcatc cggcaaacgg tcgtgctcaa agatgggaca acccatactg
    13021 aagatgtaaa aagctgcaag taatcgcagc cggcggatat ttaaccctcg gagttgccac
    13081 tgcatgcgtt ttccaggact cgtcctgatg agccgcttgc cggttaccgg ttgttatggt
    13141 cgagctttca gtgcgcctgc accgactgtc cgacctgaca ggatcactcc ttcttgcgct
    13201 tccattcatc cccgactttt tcatattcct tttttaccgc tgcccaggcg accttgtgag
    13261 cggcttcctc acgcgactcg tggcccctcc gctcgtcggg gtccttgtat tcatcccacg
    13321 cgctattgaa agccgcccgg taaatctcct cggcgtgctt cggcaatacg tgcttaacac
    13381 tatccggaag gtcgtttatc gttttgtaag gcatgttttc ctcccattgg agtgacgata
    13441 tgaatatcct tggctacagg gttaccaggc gggcagccct ttgctgcgtg tggggtctgc
    13501 cgttgagctg caagatgccg ggtccaatag aaagataggc gatacaaggg gtacaagaca
    13561 agatcggatc aatgttttga gtggcaggtt tccaacctcg gaagcgagcc ggttaaaaac
    13621 agtcaagatt aaatccttgc caagttcgta tacccgttgc cggggaaata atcacatagg
    13681 aagcaacact gcgctcccca aacccccacc ggcttgtctg ttcccgatac tcttctatta
    13741 ttatatttct atcgtggaag ccggatgccg gaatgactga acgataacaa aggaaccata
    13801 atgcattcaa tatactcccc ctcggttagc gtagtcatcc ccacttacga ccagatgcga
    13861 ttcatagtac gggcgcttga cagcctgcag gcgcaaacac tgaacgactg ggaagcaatc
    13921 gtcatagatg acggctcgcg cgacggaaca gcggaagcgg tatcgagcta cttgggcgac
    13981 gtgcgcatcc actattacca aacccctgaa aaccgtggct tgggccatgc cctgaatgaa
    14041 ggcatcagca aggcgaaggc gccgcttatc gcttacctgc caagtgatga cgtttattat
    14101 cgggatcacc tgcaaagcct gaaggcgctg cttgaaatga atccggaagc agtgttggtg
    14161 tattccggcg tctgccacca ctataaccgg ctcgcaaacg ggcagattga aggatatccg
    14221 ctgcaactgg tgcaatgcat gcaccggaaa actacggccc gctgggtgga gcgcccggaa
    14281 ctggagtcgg atgacctgga acgactctac tggtcacggc tgcggctcgc aggctcattc
    14341 gttggaacca acagtatcac ttgcgagtgg gtgtctcatc cgatgcagcg ccataagctc
    14401 atgcaggagc ctgaaggcgg catcaatcct ttccgctcgc attaccgggt ccaggaaccc
    14461 ctgcgctttc atacgtcggt tggccatgcg atcgacgagg aaagtcacta caggacgatg
    14521 cgcgaacggc cggatacgcc ctacgcaaag gatgggttga aaatactgct ggtcggcgaa
    14581 ctggcataca acgctgaccg ggtgctggcg ctcgaagagc agggacacaa gctatatggc
    14641 ttgtggatgc agaatcctca ctggtacaac acggtagggc ctttgccttt cggccacgtc
    14701 caagacctgc cgcgagacaa ctggcgtgaa gcgatcagaa agatccagcc ggacgttatc
    14761 tatgcactgc tcaactggca ggcagtgccc tttgcccacg aagtactgat ggccaccccc
    14821 ggcatcccat tcacctggca tttcaaggaa ggaccgttca tttgcctgga taagggaacc
    14881 tggtcggaac tgatcgacct gcaccagtat tgcgacggac agatattctc gagccccgaa
    14941 atgcgtgatt ggttcgatac gatcgtgcct ggtctgtcgc tgagcaaacc gactcacgtg
    15001 ctggacggag acctgccgaa gcgcgactgg tttgatcaac cgcggacgcc gttactctcc
    15061 gcaaccgacg gggaaatcca cacggtttcc ccaggccgcc cgattggctt gcaccctcag
    15121 gatgttgccg agctggcaaa gcatcgtatt cacctgcatt tttacggcga gatcacgcac
    15181 gggcagtggc tcaagtggat tgaaaaaacc cagggaatgg cgcaggggta tctgcacctg
    15241 caccccaacg tagaccaaag ccgctggacc gaggagtttt cacaatatga cgcgggctgg
    15301 ctgcacctgt ttgaaagcgg caacgggggc gaaatgcgca gggccaactg ggacgacctg
    15361 aattatcccg cgcggctgag caccctcgcg gccgctgggt tgccgatgct gcaaaaggcg
    15421 aacgacggtg cgattgtcgc cacccaaacg ttgacgaagc agcttggcat tggaatattt
    15481 tttgacgaaa ccgaggagct tgcagcccaa ttaagagatt cggagcagtt tcatgccatc
    15541 cgcgagcgag tctggcagca gcggcacctg ttcacattcg atcatcatgt gccggaactg
    15601 gtcgatttct tccgcagcgt gatcagatca gcgtccagca agccgagagg aaaaattgtc
    15661 tcggggatgg cgaggcagtc ggcgtgagtt ttctgaggat caaaaaaaat ccagattagc
    15721 gatggtgtga ttgacgaaaa cttcctgaaa ccgcttatag aattactcct ttccgcttca
    15781 tcgctcaagt tgataaaaac ttacgcctct accgggccaa gtaaccacgt tcgagatacc
    15841 atgccgccag attcacggct actatagcgt gacggaatat gctcaagtct cacattgatt
    15901 gccatccaac ccatgactca gcattttccg ggtggaggtt cccccgtctc agggtcttct
    15961 ccctgctcgc ggagacgatg tggttgccgg cgggactgaa ccggcggcaa ctcccagatt
    16021 tgctcgaaat ttaaccgaaa tttaaccgtt ttgttacaac ttttgctttt ttcgcgccta
    16081 tccttcatcg tggtcttgag gttaaaggcc cgaaccggaa cctaaattcc ttccaactct
    16141 ccagcataag gagattacat gtattctgaa atcagagaaa ttcaagaaga aattcataaa
    16201 tgcggcgcaa aatggacggc ttcggaaacg gaatttgtta aaacagttga tccagaacaa
    16261 cgtgtccatc gtttaggtgt tgtgctcgat caggaggccc tggaaaggat tcggcgacag
    16321 cccgccttag atatgccagc tttgatcgca aggtttgagc aaaaaatggg ctgggtgcct
    16381 gaaggaggcg aagcggacca tctcgatttg gtacggaaga gactcgattt acagttggat
    16441 acattgtttc cgccttggtt tccctggttt tttaacgtcg attggcgaaa ccgtaaagga
    16501 cgtaataacg ttacccctgt taaagaccaa ggtggctgcg gctctggcgt tgcttttgga
    16561 acaacgggta ccctcgaatc gatgcttctt atgaacataa tgttgatctg gatttgtccg
    16621 aagccgaact gttgaactgt ggtggtggaa gctgtggggg ctggtggccg gatagcgcag
    16681 taaactatct tttgtctcaa ggcattgctc aagaacgctg ctttccatat caacccaaaa
    16741 atatgccctg taccacgtgc gcagagcgcg acggtgaagc catccagatc agtgatcgta
    16801 ttacaattaa tgatgtcgca caacgcaggc aatacattga caatatcggt cctgtgatgt
    16861 gtgtatttga ggtatttcag gatttctatt cctactcaac ggtatctact cgcatgtttg
    16921 gggcaattcg gtaggtttac attgtgtaga agtgattgga tacaatagcc tgtttggcta
    16981 ctggatatgt aagaatagct ggggtaccgg gtttggtgag agcggttttt tcaaaatcgc
    17041 attcggtcag tgccaaattg acaccacgtt cccattttgg gggatatcag ggacgaagtt
    17101 tttcgagatc gaatcgcctt ggaaacccgt cgctggcgcg ctgaagtaca tctcggccgc
    17161 aagtgacggg tccgtttggg gggtgaacgc gggagacgag atcttccgat ggaatggtaa
    17221 cagctgggat caggtccctg gcgccctcaa gcagatctca gtaggcaacg ccgccacagt
    17281 atggggtgtg aactccgacg acgagatcta ccgctggaac ggcgcagcct gggatcaagt
    17341 gtctggtgcg ctcaaacatg tggcagtagc caacgacggt acggtctggg gcgtcaacag
    17401 cggcgataag attttccact ggaatggtaa cagctgggat caggtcccgg gcgccctcaa
    17461 gcagatctca gtgggcgcag ctaaacttgt ctggggagtc aacgagagtg acgctatctt
    17521 tagattgcga tgatcaagcg cctcgccgat accaaattga taccaccttc ccattttggg
    17581 gaatatcagg gacgaagttt ttcgggacct gagtgagtgt gctgaccacg aagatgggct
    17641 gcgaatttac cgtgcagctt aatggacagc ctaccgctgg gtatgaatgg cgttgcgagg
    17701 cgcccccccg gcgtaatcct tctttggcga gaagtacggc ctatctcatc aatggtcgga
    17761 gcctcagcgg agtatatctt ccgttttcgt gcagatgcaa ttggaacatt cttggtacat
    17821 tgcacctaca gacggccgtg ggagaagcag cccgaagaaa cacgaagtat caaggtccaa
    17881 gtggaaccga cgtaagtgca cggaggtcgc cggtcccaac tcgcccgacg cgatcaacct
    17941 cgggctgacc cagatggcag agtcctgctc cggcacgacc tacacctgac cgtcatggct
    18001 gcaagcctga cacatccgcg acgagaccta ctcaagggcg ctggcggaaa agccgagagc
    18061 accggacaca tcaacccgaa gtacaccgcg gcgatgctga cgaatgttct atacccacat
    18121 ctcggatcag tatgcgccgt tccacaccaa ggtaatcaat gtcagcgtgc gggattcgac
    18181 ctttgattac cccatgctac cttggagtat ccatgaagcc aaattccctg cgtcgccact
    18241 agcagcacac cccccatagc cgagttcgac cccgcctatt aacgaaattt aaggagaaat
    18301 catgcctctt ggtacaccgt acagcgtgat tatcggtcca ttgcatgatt tcaacctggt
    18361 caacccagat acgggccagt ggccccacta ctttccgaat atctatgact atgtcgagtg
    18421 tcaggagagg cattagctag ctagaaacga tttcaccgac cgctgggtat cagcgtccca
    18481 tttactatct gagaatttcc aagctcattt ggtgagcaaa aagatgggtg aggggttcaa
    18541 gaaaattccc taagtagctt aggcaaggcc gaaggccgcg tcaggaagat ttctttatat
    18601 ggtgaaagga aggatgccgg tgggcaatcc ctgtgggaaa cctcgtggat ggggaggtta
    18661 tccgtccatc aggtttttcc atcagagttg tccatgggct aagcgcaggc cgaatgatcg
    18721 gcaaggcagc tgctgcagta ggtatcagtg acgggtcaat cttcttaccc caaataccct
    18781 gcttccttct gatggcgaaa ggccagcaca tatatagcat catcagccgg ttcgtggcga
    18841 tataaggcta cgtcgctttc attcgtatac gaccccgccg tagctgggtc gtatacgaat
    18901 gaattctgag ccagaccaag ctaatcggcg tcttgttgtc tcttgcggcg tgccacaaaa
    18961 ccgagaagtc ccaaaccagc gagcagcatc gcataggttt cgggttcagg tacgggggaa
    19021 tagataatgt cgagtttcgg cgccagagca atgtcgccag ccgccatcgc accatctttt
    19081 gaccaaaagc ccaggagcga acgcacgatc ccttcgtgat cgcttttcag caaccagccg
    19141 ttattgtcag agggattgtt cagccagtcc tgcacatccg cagccatttg gggggagttc
    19201 catgtgtgag ttgttcccgt agcaaatgtg gtaaaagtag aggctgcgct ttcggtgggc
    19261 gatgttgtaa ttggagtaat cccaggtttg acaggccgtc gcttctaata tagccccact
    19321 ttgaggaact acaagcggga atgctcgtga ttggatagtt ggatcggtcg tccgtctagt
    19381 atcgaatccg tacgaaaagg agcagtttcg gtcatgtatg ctcgtgggtg tacgaaaaca
    19441 ggctaaatgc atttcggatg caagctataa ttcgtactcc attttcgggc gaaatcagac
    19501 ctatgccacg cgtttttgct tactgtcgcg tttcgactgc cgaccagacc acccagaatc
    19561 agagcatgga aatcaaagcg gctggattcg ccatcgaggc gcgccgactg atcgaagaaa
    19621 gcatcagtgg ctcagtgacc gcgaaggagc gacccggctt tatgaagctg gtcgaccgga
    19681 tggaaccggg cgatgtgctg gtggtgacca aacttgaccg acttgggcgg aatgcgatgg
    19741 atgtgcgggc aaccgtcgag cagctcgcga gttttggcgt gcgcgtgcat tgcctggccc
    19801 ttggggatgt tgatctaacc agcccagcag gcaaaatgac aatgcaggtt atcgccgccg
    19861 tggctgagtt cgagcgcgac ctgctgattg aacggacgca atcgggaatt attcgggcta
    19921 aggctgctgg aaaacaattt ggcaggccgc catcactcaa tatggaggaa cgcgcagccg
    19981 tcgtagagcg actggacgcc ggtgccaata ttgccgaaat tgcgcgcgaa ttcaaaacga
    20041 ctcggcaaac aattatgcgg gtaaaggaag cggctttgaa actaccgtcg caacaaaaag
    20101 agtgactcct gttttgggcg tattcggacg ggattagccg cagttagttt gattttacct
    20161 gttcgaggag ttgggcaaaa ataaccgatc ttacattttc ggcgggatga gcaatattag
    20221 cctaactaga ggtaaagaca cgattcgcgc tttaaagcac gctaagaacc accaacgcat
    20281 caatcgcgcg cgtacacagt gaatcaaggg ccctgcaatt tctgattgcg aaatttaacc
    20341 gaaatttaac gttttgttac aacttttacc tttctcttgc ctatccttca gacgtggagc
    20401 aatgaaattg ttctctatgg atacaaaacg ataaggaggt tgaaatgtct tcaaataacc
    20461 agtttacagc tttaatgctc ggtcaacgaa agagagtaag ggtgtgtcca ttatcaatgc
    20521 cttcaaaaca aaggctataa cttgggtttt atttttcgtt ttttcgacaa gtgtattcgc
    20581 ccaagctacc gaaggtgcga ggataacaaa cgatagcata gccaattttt cgataacggt
    20641 tgggcatggc ggggttattg acgttgcaga aggacgcgac gcgtcttatt ttggtgtgac
    20701 aatgagcggt ttttacaact atgattatca gaatcccacc gaaacaccga attagtgcca
    20761 gtccgaggaa ccgttagtta cggacaccag gttgacgact taggactact cgtttatctt
    20821 ttcccggtag gttcgaatga ggttgtgcgc ggggagcagt cgttttcttt agttgccggc
    20881 cccttccagt tctaacctga agaacaatac tcagttgttt cgcaggtggg tcaccgtatc
    20941 ggcatccaga cgttttcggt ggtagtagtt ccagaaccga agacttacgc tttgatgctg
    21001 gcaggactag gactgctg
//
LOCUS       DASSHJ010000171         6006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1381667,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000171 DASSHJ010000000
VERSION     DASSHJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381667"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFI43_06610"
     CDS             <1..916
                     /locus_tag="VFI43_06610"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HET7061832.1"
                     /translation="AEPREAEGAISETPVEGTPNSRRAKEEPSPSPAGQDEVTASAEN
                     RLQRGETTGETGSAESNEAEFNEEVRSHETEARGRDGNAPRPDHAGESPAAATAARET
                     QTQHERPHDRSREQGHEVDAYAESAHAGAYAFEAEQSEIASFAWSLGCLLLLIIFTGQ
                     TIYFYSADLAKAAPAAKPYLEQYCELMGCTVSPARPQQLLDIESSDMRSDPLRPSIIT
                     LNATVRNHALYPQALPLFELTFIDAENRSLASRIFRPDMYLPPDANRMESIPPGKEFN
                     VKLHLDTTDLNPAGYRLSLLYPPPDPQA"
     gene            1028..1840
                     /locus_tag="VFI43_06615"
     CDS             1028..1840
                     /locus_tag="VFI43_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061833.1"
                     /translation="MASETLVEIKDLNFSYGERPVLKGINMTMARGKVIAIMGGSGSG
                     KTTLLRLIGGTIRPSSGYVKVAGKEVDKLSRDELFRLRRKMSMLFQFGALFTDLSVFD
                     NVAFQMREHTDLPESMIRDLVLMKLQVVGLRGAQELMPGELSGGMARRVALARSIALD
                     PLLIMYDEPFTGLDPISLTVIGNLIRRLTDALGMTSIVVTHDVQESLKIVDYVYFVAD
                     GVVAAEGTPAEVRKSDAPFVHQFVHGEEDGPVSFHYPAKAYAADLKLEARVA"
     gene            1833..2630
                     /gene="mlaE"
                     /locus_tag="VFI43_06620"
     CDS             1833..2630
                     /gene="mlaE"
                     /locus_tag="VFI43_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HET7061834.1"
                     /translation="MPSRIVEGIRGIGHRAVDSVWRLGYAARFFTLTLLKSGTSLRRF
                     GLVVREIYFTGVLSLIIIIVSGLFVGMVLGLQGYETLQRYGAESAVGTMVALSLLREL
                     GPVVSALLFASRAGSAITAEIGLMKATEQLSAMEMMAVDPIARVVAPRFWSGVISMPL
                     LAGLFSAVGVFGGYMVAVVLIGVDEGSFWSQMQDAVDFRYDIVNGIIKTFVFGIAVTA
                     IAVFEGYDASPTAEGVSGATTRTVVTSSLAILGLDFVLTSFMFRGMS"
     gene            2630..3130
                     /gene="mlaD"
                     /locus_tag="VFI43_06625"
     CDS             2630..3130
                     /gene="mlaD"
                     /locus_tag="VFI43_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET7061835.1"
                     /translation="MQRAAMDLWVGLFVIAGIGALLLLAFKVGNLGTYSAGDSYILKG
                     NFENIGGLKIRAPVKGAGVVVGRVKDIQFSTQTYDALVTLSIDGRYKFPKDTFASILT
                     SGLIGEQYIGLSAGGDEAMLKDGDKIMKTNSAMVLEEMIGRFLFDKASENKSSDDDPG
                     ESGSSK"
     gene            3190..3834
                     /locus_tag="VFI43_06630"
     CDS             3190..3834
                     /locus_tag="VFI43_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7061836.1"
                     /translation="MKKYLSIIPIVLGWLLASPAWSIERPDTLVDNTAQEVLTMIRQD
                     KELRGGNHGKLLNLIEVKILPHFDFTRMTRLAMGKNWVKATPEQQQTLVNEFRTLLVR
                     TYSNALSTYSDHTIKVEPLKTVPDSDTLVKTKVVQDSGQEPIPIDYSMEKTGDGWKVY
                     DVTVAGVSLVTNYRSTFNTQVRDGGVEKLIKTLADKNKSLLAADGAAEKKAASR"
     gene            3879..4241
                     /locus_tag="VFI43_06635"
     CDS             3879..4241
                     /locus_tag="VFI43_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7061837.1"
                     /translation="MAADEFAEVRVGEEGRLVVSGPVTIENVVAVMEQGTALFDRQDF
                     TVDLGGITDVDSSAASLLLHWEREALDRNQRLVFVNIPQKLQTLLRLYGISGLINGNA
                     SRPDPAAFAETASSQRGG"
     gene            4347..5270
                     /locus_tag="VFI43_06640"
     CDS             4347..5270
                     /locus_tag="VFI43_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061838.1"
                     /translation="MPSAIQVKQMRKRFGSVQALAGVDLEIHEGEFFALLGPNGAGKT
                     TLINIIAGLMRATSGSAFVMGHDVVRDYREARRMLGVVPQELVYDPFFTVRETLTIQS
                     GYYGFRNNGDWIDEIIHHLDLDDKANANMRSLSGGMKRRVLVAQALVHRPPVIVLDEP
                     TAGVDVELRQGLWHFIKQLNHDGHTIVLTTHYLEEAEVLCNRVAMLKHGNVVALDSIK
                     NLVKSVPGCDVRMRLSPDELPSALQPLQKAYEDGYRVLSLKDYAQLEEVMASLRVAGC
                     KVLEMQVLQPDLEEVFVKIMSQADSAKATLI"
     gene            5309..>6006
                     /locus_tag="VFI43_06645"
     CDS             5309..>6006
                     /locus_tag="VFI43_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7061839.1"
                     /translation="MNGFSGFPTLLYKELLRFWKVGLQTIFAPIVSVLLYLMIFSHAL
                     QEHVQPYPGVAYAIFLVPGLIMMAVLQNAFANSSSSIIQSKVTGNLVFVLLSPLSYQE
                     IFFAYVLASIVRGLAVGLGVYLVTLWFFELSLSSLAWVFIFALMGSALLGALGIVAGI
                     WADKFDQLAAFQNFVILPLTFLSGVFYSIHSLPPFWQGLSHLNPFFYMIDGFRYGFFK
                     VSDISPYVSLGIVTA"
BASE COUNT         1325 a         1547 c         1727 g         1407 t
ORIGIN      
        1 agctgagccg agggaagcag agggagcaat ttcagaaaca ccggttgagg gaacgcccaa
       61 ctcgcgaagg gcaaaggaag agccttctcc aagcccggca ggccaggatg aggtgactgc
      121 atctgctgaa aatcgattgc agagggggga gacaactggg gagacaggca gcgccgagtc
      181 caatgaggct gagttcaatg aggaagtgcg aagccatgaa actgaagcaa ggggaagaga
      241 cggaaacgct cccaggcctg atcatgcagg agaatcgcct gcggctgcca cggcagccag
      301 ggaaacacag acacagcatg agcggcccca tgataggtcg cgcgagcagg gtcatgaggt
      361 agacgcctac gcggagtccg cgcatgccgg agcttacgct tttgaagccg aacagtcgga
      421 gattgcctcg ttcgcgtgga gccttggctg cctgcttctg cttatcatat tcactgggca
      481 aacaatttat ttttacagtg ctgacctcgc aaaggccgcc ccggcagcca aaccttatct
      541 tgaacagtat tgtgaattga tgggatgtac tgtctctcca gcccggccgc aacaacttct
      601 ggacatcgaa tcatcggaca tgcgatccga tcccctgcgt ccaagcatca tcacgctgaa
      661 tgcgacggta cgtaatcatg ccctttatcc tcaagcattg cctttgtttg aacttacttt
      721 catcgacgct gaaaaccgct cgctggcgag ccgtattttt cgtcccgaca tgtatcttcc
      781 acctgacgcc aaccggatgg aatcgatccc tcccggaaaa gagttcaacg tcaagctgca
      841 tttggatacc actgatctca acccggcggg ctatcggctc tccctgcttt atccgccgcc
      901 tgatccgcaa gcctgaagat ttgtcatgac tttctgctca tcagcagtcg agcattactc
      961 gtctgaccag cctccggttt taggttaaaa tcgaacccct ttcaaagttg ggtgtactgc
     1021 cggaagcatg gcctctgaaa cactggtaga aataaaggat ctcaattttt cctatggcga
     1081 gcgcccggtt ctgaaaggga tcaatatgac catggcgcgg gggaaggtga tcgctatcat
     1141 ggggggcagc gggagcggca agacgacgct gctgcggctc atcgggggca ccatccggcc
     1201 ctcgtccggc tatgtgaaag tggcgggaaa ggaggtggat aagctcagca gggatgagct
     1261 cttccggttg cggcgcaaga tgagcatgct cttccagttc ggtgcccttt ttaccgatct
     1321 gtccgtgttc gacaacgtcg cttttcagat gcgggaacat accgacctgc cggaatccat
     1381 gattcgggat ctcgtgctga tgaagttgca ggtggtcggc ctgcgcggcg cccaggaatt
     1441 gatgccgggg gagctttccg gagggatggc gcgccgggta gccctggccc gttccatcgc
     1501 gctcgatccc ttactgatca tgtatgatga accgtttacc gggctcgacc ccatctcgct
     1561 gacagttatc ggaaacctta tccgtcgctt gacggatgcg cttggaatga cctctatcgt
     1621 ggtcacgcac gatgtccagg agtcactgaa gattgtcgat tacgtgtatt tcgttgctga
     1681 cggagtggtc gcggccgaag gcacgcctgc ggaggtgcgg aaatcggacg ccccctttgt
     1741 gcatcagttc gtgcatgggg aggaagacgg cccggtctcg tttcattatc cggcgaaagc
     1801 gtacgcggcc gacctgaagc tggaggcgcg cgttgcctag tcgcattgtc gagggcatcc
     1861 gggggatcgg tcaccgggcc gtggatagcg tctggcggct gggctacgcg gcgcgttttt
     1921 ttacgctgac cctcctcaag tccggtacca gcctgcgccg cttcggcctc gtagttcgag
     1981 aaatttattt taccggcgta ctttccctca tcatcatcat cgtgtcagga ttgttcgtcg
     2041 gaatggtgct tggcttgcag ggttacgaga cgttacagcg atacggggct gaatcagccg
     2101 tagggacaat ggttgcgtta tcgctgttga gggagctcgg gcccgtagtg tctgcgttat
     2161 tgttcgccag tcgtgcgggc tcggcaatca ctgctgaaat agggttgatg aaggccaccg
     2221 agcaactctc tgccatggaa atgatggcgg tggatccgat tgcgcgagtg gttgctccaa
     2281 ggttctggtc cggagtgatt tccatgccac tccttgccgg tctcttttcc gcggtcggcg
     2341 tgtttggcgg ctacatggtg gccgtcgtac tcatcggagt ggatgagggg tcgttctggt
     2401 cccagatgca ggatgccgtg gatttcaggt acgatattgt caatggcatt ataaaaacgt
     2461 ttgtgtttgg tatcgcggtc acggcaatcg cagtgttcga gggttacgac gcatctccca
     2521 cggctgaagg cgtgtccggc gcgaccactc gcaccgtcgt aacttcctcg ctggccatcc
     2581 tcgggctcga ttttgtcctg acctcgttca tgttcagggg aatgagctga tgcagcgggc
     2641 ggcaatggat ttatgggtgg ggctgttcgt cattgcggga atcggggctt tattgctgtt
     2701 ggcattcaaa gtcgggaatc tcggtacgta cagtgcgggg gacagctaca tcctcaaggg
     2761 aaatttcgag aatataggcg gcttgaaaat cagggcaccg gttaaaggcg caggggtagt
     2821 ggtagggcgt gtaaaggata tccagttcag tacccagact tacgacgcat tggtcacctt
     2881 gagcatagat ggccgttaca agtttccaaa agacacgttc gccagtatcc ttacgtccgg
     2941 tctcataggc gagcaataca tcggcctttc ggcgggaggg gatgaagcca tgctgaagga
     3001 cggcgataaa ataatgaaga ccaactcggc catggtgttg gaggagatga tcgggcggtt
     3061 tctgttcgac aaggcctcgg agaacaaatc ttccgacgac gatccgggcg aaagcggaag
     3121 cagcaaatga agtggattta agggcgtata tgtttgcctt ttttatacag taaaaatcgg
     3181 tgaggaaata tgaaaaagta tctcagcatc atccccatag tgctaggttg gttgcttgcg
     3241 tctcccgcat ggtccatcga acggcccgat actctcgtgg acaacacggc acaggaagtg
     3301 ctcaccatga tcaggcagga caaggaattg cgcggcggca atcacggcaa gctccttaac
     3361 ctgatcgaag tgaagatatt gccgcatttc gactttaccc ggatgacacg cctcgcgatg
     3421 ggaaagaact gggtaaaagc aacgcccgag cagcagcaaa cactcgtgaa cgagttccgt
     3481 acgctcttgg tcaggaccta ttccaatgcc ctgtccacct atagcgacca tacgatcaag
     3541 gtcgagccgc tcaaaactgt tccggattcg gatacgttgg tcaagacaaa agttgtccag
     3601 gacagtgggc aggaaccgat cccgatcgat tacagcatgg aaaagaccgg cgacgggtgg
     3661 aaggtatacg atgtgacagt cgctggagtg agccttgtca cgaactatcg cagcaccttc
     3721 aatacccagg ttcgggacgg cggcgtggaa aagctgatca agacactggc cgataaaaat
     3781 aaatcgctat tggccgccga cggcgcagcc gaaaaaaagg ccgcatccag atagcaggtt
     3841 agtcgcgaaa ttcctacaag actgaaagct gcatcagtat ggcggcagac gaatttgctg
     3901 aagtgcgagt gggtgaagag ggcagactgg tcgtcagcgg acctgtaacg atcgagaatg
     3961 tcgtcgcagt aatggagcaa ggcactgctt tatttgaccg ccaggacttc acagttgatc
     4021 ttggaggaat tacggacgtg gactcgtcag ccgcaagttt gttgcttcat tgggagcgcg
     4081 aggccctcga tcgcaatcag cggctcgtct tcgtcaatat tccgcaaaag cttcagaccc
     4141 tgttaaggct gtatggtatt tccggactga tcaacggaaa tgccagcaga cctgatccgg
     4201 cggcgttcgc tgaaactgct tcttcgcagc ggggcggttg agaaactact gctctcctgc
     4261 gcagaatccg cgcaatagtt gcgtgcgtcg ttttcctcat ttgttcctcc agcatctttg
     4321 ttcctcggtc gttctcactt ttatccatgc cttctgcaat tcaagtcaag cagatgcgca
     4381 agcgctttgg ctcggttcaa gcgctcgctg gcgtcgatct cgaaattcat gaaggtgaat
     4441 tcttcgcctt gctggggccc aacggggctg gcaagaccac gctgatcaat atcattgcag
     4501 ggctgatgcg ggctacgagc ggcagcgcgt ttgtaatggg gcatgatgtc gttagggatt
     4561 accgtgaagc gcgccgcatg ctgggagtag tgccgcagga gctggtatat gatccattct
     4621 tcactgttcg cgagactctc accattcagt ccggctacta tgggttcagg aataacggcg
     4681 attggataga tgagatcatt catcacctcg accttgacga caaggccaat gcaaacatgc
     4741 gctccctgtc cggagggatg aagcgccgcg tgctagtcgc ccaggcgctg gtgcaccggc
     4801 cgcccgtgat tgtgctggat gagcccaccg ccggcgtaga tgtcgaattg cgtcaggggc
     4861 tgtggcactt catcaagcaa ttgaatcacg acggacatac catcgtactg accacccact
     4921 atctggaaga agcggaggtc ttgtgcaaca gggtggcaat gctgaagcac ggcaacgtgg
     4981 ttgcactcga cagcataaaa aatctcgtca agagtgtgcc aggatgcgat gtccgcatgc
     5041 gtttgtcgcc cgacgagtta ccttcagccc tgcaaccgct acagaaagcc tatgaagatg
     5101 gatatcgcgt tctctcgctt aaggactacg cacagcttga agaggtcatg gcgtccctgc
     5161 gagtggcggg atgtaaagta cttgaaatgc aggtattgca gccggacctg gaagaggtat
     5221 tcgtaaaaat catgagccag gccgatagcg ccaaggcgac gctcatttga ttgcgagcaa
     5281 aaagcatgaa agaaagaaca tcggccatat gaacggattt agtggatttc ctaccctgct
     5341 ttataaagag ttgctgcggt tctggaaagt cggactccag accatatttg cgcccatcgt
     5401 atccgttctg ctctatctca tgattttttc ccacgcgttg caagagcacg tgcagccata
     5461 ccccggggtt gcctacgcaa ttttcctggt tccaggactg atcatgatgg cggtcctgca
     5521 aaatgctttt gccaactctt catccagcat tatccagtca aaggtgacag gcaatctcgt
     5581 ctttgtgctg ttgtcgccgc tttcctacca ggaaatattc ttcgcctatg tactggcgtc
     5641 gatcgttcga gggcttgcgg tggggttggg tgtttacctg gtcacgctct ggttcttcga
     5701 actatcccta tcgtctttag cgtgggtatt catttttgcc ctgatgggca gcgcgctgct
     5761 cggagcactc ggaatcgtcg cagggatctg ggccgacaaa ttcgaccaac tcgctgcgtt
     5821 ccagaacttt gtcatcctgc cattgacgtt cctgtccggc gtattctatt cgatccattc
     5881 gttgccgccc ttctggcagg gcctttcaca cctcaaccca ttcttctaca tgattgacgg
     5941 attcaggtat ggcttcttca aggtttcgga tatttccccc tatgtcagcc tcgggattgt
     6001 aactgc
//
LOCUS       DASSHJ010000172         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_119161, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000172 DASSHJ010000000
VERSION     DASSHJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119161"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..889)
                     /locus_tag="VFI43_06650"
     CDS             complement(380..889)
                     /locus_tag="VFI43_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061840.1"
                     /translation="MKNMPGKKTVVSLALGSAFAVALGAAPIASAGENPFAAQSVTKG
                     FMLADAHGDRGYGKSGEGRCGMSMADTDNDGRVSADEHARHCQVMFEKMDTNKDGFID
                     KDEAAKMRKMHGHGHRGGGYGSRERSSGYSGDQSSRSGYARESMSGYNEYGTRTGDNE
                     LQHMKPMGQ"
     gene            1315..2181
                     /locus_tag="VFI43_06655"
     CDS             1315..2181
                     /locus_tag="VFI43_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7061841.1"
                     /translation="MNDWEKLYTQAESLLSRLEGLVSGEKPETDWNAAVAFRWRKHGR
                     IQGLIQPVPHPHHISLESLRGIDAQKRLVDQNTRQFVTGHPANNVLLTGARGTGKSSV
                     VKAMLTKYASTGLRLIEVEKHDLIDLHDIVDQVCERPERFIIYCDDLSFESGEPGYKA
                     LKVVLDGSIATSSDNVLVYATSNRRHLMPEFMQENRDTRHVGNEIHPSEAVEEKIALS
                     ERFGLWVSFYPFDQDQYLEAVAQWLRYFQSAELTPEVRQQALQWALGRASRSGRVAWQ
                     FARDRAGKDGAA"
     gene            2255..3262
                     /locus_tag="VFI43_06660"
     CDS             2255..3262
                     /locus_tag="VFI43_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HET7061842.1"
                     /translation="MESSAATPLGYSAIVEVAVAVILRADGSFLLARRPEGKPYAGYW
                     EFPGGKVQPGESLLHALKRELWEELDITVEHAYRWLTRTFTYPHATVRLCFFRITEWR
                     GPPRPCENQQLSWQYSGRVEVEPLLPANAPVLRALELPVVYAVTNALEAGTEGIESGL
                     AAVERALHAGARLIQIREKRLKGEMLRRFASETVRHACAQQAIVVVNGDTGDADFCRE
                     IGADGLHLPSHQLMSLHARPDVTWCGASCHNAEEIFRAEQLSMDFIVLGPVLPTLSHP
                     YSSVLGWRKFGKLIENCSLPVFALGGLRSEDLETAWEAGAHGIAMMSAFSPQHPYSGN
                     S"
     gene            complement(3259..3483)
                     /gene="yacG"
                     /locus_tag="VFI43_06665"
     CDS             complement(3259..3483)
                     /gene="yacG"
                     /locus_tag="VFI43_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET7061843.1"
                     /translation="MGNSNNRQAEKRPIVNCPQCGKVVVWETGNSYRPFCSERCKLID
                     LGQWAAESYRIPESEKQEGELPDEWEDQKN"
     gene            3899..4504
                     /gene="petA"
                     /locus_tag="VFI43_06670"
     CDS             3899..4504
                     /gene="petA"
                     /locus_tag="VFI43_06670"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET7061844.1"
                     /translation="MADIDKKQEMNGRRRFLVAATSIAGGIASIGVATPFVLSMMPSE
                     RAKAAGAPVEVDITKIEPGMLLLVEWRGKVVWVLDRTPEMLATLKKLDSQVADPKSEK
                     PQQPPYCQNETRSIKPAILVVEGVCTHLGCSPVYRKDIAPADLGPDWLGGFFCPCHGS
                     KFDLAGRVYKSVPAPTNLVVPPHMYLSDTRLLVGSDKKESA"
     gene            4505..>5298
                     /locus_tag="VFI43_06675"
     CDS             4505..>5298
                     /locus_tag="VFI43_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HET7061845.1"
                     /translation="MGKHLESMMEWIDSRFPLTSTWKAHLSEYYAPKNFNFWYYFGSL
                     AMLVLVNQLLTGIFLTMNYKPDASMAFGSVEYIMRDVDFGWLIRYMHSTGASMFFVVV
                     YLHMFRGMMYGSYRKPRELLWLIGMGIFFVLMMLAFTGYILPWGQMSYWGAQVIISMV
                     GAIPVVGQTLSNWILGDFMLSDAALNRFFAYHVVTLPVLLVTLVFVHIIALHEVGSNN
                     PDGIEIKANKDPVTHIPVDGIPFHPYYSVKDIVGVVGFLIVFCGII"
BASE COUNT         1181 a         1465 c         1394 g         1258 t
ORIGIN      
        1 atttgtcgta tgactacgtg agaatcaacg caagctaccg aagttagatt aaggagcagg
       61 aagtcggaat tcaccctctc tccctcaagg agggagggag ggcgaaaagt agctgcattt
      121 aggagaccgg gccgcgtatc cctttgttgt ccgccggatt cgctggtccc cgactacact
      181 ctggtaccta cactcacggg gagcctcacg ctgcgctcca ggatgcagcc gcgaagggcg
      241 aagcagtgat cctgtgcata aagatgcaac cgacataaag atgcaaccga taatgagatt
      301 cccttcatct caccctttat cagggcggca tccccttcag ctgcatgacc gcttcgcggc
      361 ccctcatggc gctctagcgc tactgcccca ttggcttcat gtgctgcaac tcgttgtcgc
      421 cggttcgggt gccatattcg ttatagcctg acatggattc cctggcatag ccggatcggc
      481 tagattgatc gccgctgtat ccggacgacc gctcacgcga gccatacccg ccgccacgat
      541 gaccatgacc atgcattttt ctcattttag ctgcctcatc cttgtcgatg aatccgtcct
      601 tgttggtatc catcttctcg aacatgacct gacagtggcg ggcatgctcg tcagcgctta
      661 cccggccatc gttgtcggtg tccgccatcg acatcccgca ccggccctcg ccggacttgc
      721 cgtaacctct atctccatgg gcatcggcga gcatgaatcc cttagtcacg gattgggcag
      781 caaatgggtt ctctcctgct gatgcaatag gggcagcgcc caacgcaacg gcaaaagcag
      841 aacctaaagc aagagacacg accgtttttt ttcctggcat atttttcatg atgtttatct
      901 ccttagtgtt ttgcacgatt tgagaaatag cacgcgccta cgcttacccg tgcgccaatt
      961 ccacaagcta tcgttccatc aagccatgat gggatacagg gccaggttgt tggtcagtat
     1021 tagcaattgc gcgagcaatc cagtacgata cgaaaccttg ccgccgttca catgctcggc
     1081 gtagggtaca catcattgct tttctagctg aacggttcag tgcgttaaca cacataaata
     1141 tccaccaata gagaatcgcc tacgaggtaa gcgataccag gttttctacc agaggaaacc
     1201 tggatgtgaa gcgagtgcaa taagaaacgt caaggagtaa cggccggggg attgaagcga
     1261 taggtcctga cgcttcacgc aaccatgact cttgttttcg caaaccggtt ttcaatgaac
     1321 gattgggaaa aactgtatac ccaggccgaa agcctcctct cccgcctgga agggttggta
     1381 tccggcgaga aacctgaaac agactggaac gctgccgtgg cttttcgctg gcgcaaacat
     1441 ggccgcatcc agggactcat acaacccgtc ccgcaccctc accatatttc cctggaatcg
     1501 ctgcgtggca ttgacgccca gaaaaggctg gtggatcaaa acacccggca gttcgtaaca
     1561 gggcatcccg ccaacaacgt cctgcttacc ggcgcgcgcg gcaccggcaa gtcgtcggtg
     1621 gtcaaggcga tgcttaccaa atatgcgtcg accggtctcc ggttgataga ggtggaaaaa
     1681 cacgatctga tcgatctgca tgatattgtt gaccaggtgt gcgagcgccc cgagcgcttc
     1741 attatttatt gcgacgactt gtcgttcgaa tcgggtgagc cgggttacaa ggccctgaag
     1801 gtggtgctcg acggttccat cgccacgtct tcagataatg ttcttgttta cgcaacatcc
     1861 aaccggcgtc acctcatgcc tgagttcatg caggaaaatc gtgatacgag gcacgtggga
     1921 aatgaaattc atccgagcga agccgtggaa gagaaaattg cactatccga gcgcttcggt
     1981 ctatgggtct ctttctatcc attcgaccag gatcagtacc tggaagccgt tgctcaatgg
     2041 ctccgttact tccaatctgc ggaactgaca cccgaggtgc gtcagcaagc gctgcaatgg
     2101 gcgttgggac gcgcctcccg aagcggacgc gtggcgtggc aatttgctcg tgatcgggcg
     2161 ggaaaagatg gtgcagcatg ataattctca ttgacaggat ttcaatctca ctccagcctt
     2221 caattgcctg atcctcgaca gcgaattcct caccatggaa tcatcggccg ctaccccctt
     2281 aggctattcc gctatcgtag aagttgcggt tgcggtcatt ctcagggcag atggctcttt
     2341 tctcctcgcc cgcaggcctg agggaaaacc ctacgcgggg tactgggagt tccccggggg
     2401 caaagtccag ccaggagaat ccttgctgca cgctctgaag cgcgagcttt gggaagaact
     2461 cgacataacg gtcgaacacg cctaccggtg gcttactcgc acattcacct atccccatgc
     2521 aactgtccgt ctttgttttt ttcgcataac ggagtggcgg ggcccgcccc ggccctgcga
     2581 aaatcagcag ctttcgtggc aatactcagg tcgcgtggaa gttgaaccgt tgcttcccgc
     2641 caatgcgccg gtcttgcgag cgcttgagtt gcctgtggtc tatgccgtca caaatgcttt
     2701 agaggccgga accgaaggga ttgaaagcgg gttggcagca gtcgaacggg ctcttcatgc
     2761 aggagctcgg ctgatccaga ttcgcgaaaa acgcttgaaa ggtgaaatgc tgcggcgatt
     2821 cgcaagcgaa acagtccgcc acgcatgcgc gcaacaagct attgtcgtgg tcaatggcga
     2881 tactggtgac gccgatttct gccgcgaaat cggagcagac ggactacacc tgccttcgca
     2941 ccaactaatg agcctccacg cgcgccccga tgtcacatgg tgcggagcgt cttgccacaa
     3001 tgcagaggaa atcttccgcg ccgagcagtt gagcatggac ttcattgtgc ttgggcctgt
     3061 actaccgacc ttgagccatc cttattcttc ggtactgggc tggcgcaagt tcggaaaact
     3121 catcgagaac tgctcgctcc cagtattcgc actgggcggt ttacggtcgg aggatctgga
     3181 gacagcttgg gaggcaggcg ctcacggaat tgcgatgatg agtgctttct cgccgcaaca
     3241 tccgtactcg ggcaatagct aatttttttg gtcttcccac tcatccggca attcgccttc
     3301 ctgtttttcg gattccggta tccggtagga ttccgccgcc cattggccta aatcgatcag
     3361 cttgcaccgc tctgaacaga acggccggta gctattgcca gtttcccaca cgacaacctt
     3421 gccgcactgc gggcagttga cgataggcct cttctcagcc tgcctgttat tgctgttgcc
     3481 catgttccca ctcgtataag tcgtacacaa gtcgtactat tgcaattttt taacagcctg
     3541 ctggaaggtt tatcgcggcg caggcaataa gaacccgggt cattgaaaac attataggtt
     3601 aaaaggtgga tggaaagacc cgctggacag cccgcccggt gtaacgaagt tcagcatgaa
     3661 aatttgtgaa gacattcttt ttgtgaccct acggcgcgcg cgcatatcgc ggcagccaac
     3721 tcatcgccaa acatcagtcc cagtggaatt ttttccgtcc aatcaatgtg ttttatgagc
     3781 agatgtttat ttcctaatac ttactatgtt aaaatggtta agtttttttt ttaacctcaa
     3841 ccaggctttt taccgttaaa aacggggagt ccgaattcgg aaaacggaac tgcaaaatat
     3901 ggcggatatt gataaaaaac aagaaatgaa tgggagaagg cgttttcttg ttgccgcgac
     3961 ttccatagcg ggcgggatcg caagcatagg ggtcgcaaca cctttcgtgc tgagcatgat
     4021 gccgagtgag cgcgcgaagg cagcaggagc ccctgtcgaa gtggacatca ccaagattga
     4081 gcctggcatg ctgcttctgg tggaatggcg agggaaagtg gtgtgggtac tcgatcgcac
     4141 cccggaaatg ctggcaaccc tgaaaaagct tgatagccag gttgccgacc ccaaatccga
     4201 aaagccccag cagccaccct actgtcaaaa tgaaacgcgg tcaatcaagc ctgccattct
     4261 ggtggtggag ggagtatgca cccacctcgg atgctcaccg gtataccgca aggacatcgc
     4321 tccggccgat ctcggtcccg actggctggg aggctttttc tgcccttgcc acggctccaa
     4381 gttcgatctg gccggacgtg tttacaaaag tgttcccgcc ccgaccaacc tggtggttcc
     4441 gcctcatatg tacctcagcg acacccggct gctggtgggg tccgataaaa aggagtcagc
     4501 gtaaatgggc aagcaccttg agtcgatgat ggaatggatt gattcgcgtt tcccgttgac
     4561 ttcaacctgg aaagctcacc tttccgaata ctacgcgccg aaaaacttca atttctggta
     4621 ctacttcggc tcactggcaa tgctggtgct ggtcaatcag cttcttaccg gcattttcct
     4681 caccatgaac tacaagccgg atgccagcat ggcgttcggc tcggtcgaat acatcatgcg
     4741 ggacgtcgat ttcgggtggc tgatacgcta catgcactcc accggcgcct cgatgttttt
     4801 cgtggtagtt tacctgcata tgttccgcgg aatgatgtac ggctcctatc gcaagccgcg
     4861 cgaactgctc tggctgatag gcatgggaat ctttttcgtt ctcatgatgc tcgcgtttac
     4921 tggctacatc ctgccatggg gtcagatgtc ctactggggt gcccaagtga ttatcagcat
     4981 ggtcggggcc atcccggtgg tcggtcaaac cctgtcaaac tggattctcg gcgatttcat
     5041 gttatcggat gcggcgctca accggttttt cgcttaccac gtcgtaaccc tccctgtttt
     5101 gctggtgacg ctggtattcg tccacatcat cgcgctgcat gaagtgggct ccaacaaccc
     5161 tgacggcatc gagatcaagg cgaacaagga ccctgtgacg cacatcccgg tcgatggcat
     5221 tcccttccat ccctattact cggtaaagga tatcgtcggc gtcgtcggat tcctgatcgt
     5281 gttctgcggg atcatatt
//
LOCUS       DASSHJ010000173         5013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1444879,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000173 DASSHJ010000000
VERSION     DASSHJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444879"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFI43_06680"
     CDS             complement(<1..530)
                     /locus_tag="VFI43_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET7061846.1"
                     /translation="MKSSEIRQKFLEFFESHGHAVVPSSSLVPGNDPTLLFTNAGMVQ
                     FKDVFLGHEKRPYVRATSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RHAIKFAWEFLTVTLDIPAEKLWVTVYAEDDEAADIWLNEIGIDPARFTRIPTQDNFW
                     QMGDTGPCGPCSEIFY"
     gene            complement(581..949)
                     /locus_tag="VFI43_06685"
     CDS             complement(581..949)
                     /locus_tag="VFI43_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061847.1"
                     /translation="MTKKTMRTFMPIRAARAAALASALVFSGCGINATPVGDIAKSPA
                     NFDGKEVTLHGTAKDQTRIPLIDMKSYVLKDNSGEIMILTNGDLPKADEKLTVKVKVQ
                     NLAIVNGESLGMAATEISRR"
     gene            complement(949..1425)
                     /gene="recX"
                     /locus_tag="VFI43_06690"
     CDS             complement(949..1425)
                     /gene="recX"
                     /locus_tag="VFI43_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET7061848.1"
                     /translation="MRTAASKARTTGQGPSLKHRALAHLARREHSRLELEKKLAPHAG
                     TPEELSAILDELERAGWLSPARVVEQVIHTRKRRFGSQRIAYELREKGIPENLIAEAL
                     PNLKEAEQVTAQEVWRKKFGVMPGNAKEFVRQTRFLLGRGFSSAVIARVLRNKEDE"
     gene            complement(1496..2512)
                     /gene="recA"
                     /locus_tag="VFI43_06695"
     CDS             complement(1496..2512)
                     /gene="recA"
                     /locus_tag="VFI43_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7061849.1"
                     /translation="MDDNRSRALAAALSQIEKQFGKGSIMRLGASDVAKDVEVISTGS
                     LGLDIALGVGGLPRGRVIEIYGPESSGKTTLTLQVIGQMQKMGGTAAFIDAEHALDPQ
                     YAQKIGVNVQELLISQPDNGEQALEIADMLVRSGSIDVVVVDSVAALTPRAEIEGEMG
                     EPQMGLQARLMSQALRKLTANIKRSNTMVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRTGSIKKGEEVIGSETRVKVVKNKVAPPFKQAEFDILYGEGISREGEIVE
                     LGVQHKLIDKSGAWYAYKGDKIGQGKDNAREFLKEHHDIAMEIESKIRAAVGVSNPAE
                     AVHA"
     gene            complement(2672..4024)
                     /locus_tag="VFI43_06700"
     CDS             complement(2672..4024)
                     /locus_tag="VFI43_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HET7061850.1"
                     /translation="MHHDSFSNRCAKRVERHSCLALLGGFASALLSSLAPADTAKVDE
                     QVFSGVRDSVVAVETFDARGKRVAGGSAVAIGEGEVVTSCTLIRGGASIHIREAGKAF
                     KAVVRHKRQDLDLCELDVAGLGIRPVGLAGSKKPWAGERVYAVGISSKQEPQPVIHSA
                     KISGLLPHEGSWYMQVSAVPSQGFEGGGLFSDSGQLIGILAFRHVEGQNLAFALPVDW
                     LKELEKKVTPASTKKENGLEWLNRCFALEKKGDWRGLLKLSQLHVERDQDSAAGWFEV
                     GAASIHLKQYRQAVHAYRQAIRHQARYGEAWHGLGMAYMHLKEYEHATRALQDAVRLQ
                     PENAEAWYELGNAYHKSEKYPHAIHAYDQSLRFKPENATVWYQLGKTYDELDLYGDAI
                     EAYRETVRIQPKNVDALYSLGVDYAIAGERAEMRKVYAALRGLDPVKAELYFKTYILP
                     "
     gene            4188..4742
                     /locus_tag="VFI43_06705"
     CDS             4188..4742
                     /locus_tag="VFI43_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HET7061851.1"
                     /translation="MAYFIFTTRWLQLPLYLGLILAQCVYVYHFWVELIDLIGAVMGN
                     QAALQHILDAVAIEGAPRPSKINETTIMLVVLGLIDVVMISNLLIMVIIGGYETFVSR
                     MNLEGHPDQPEWLSHVNASVLKVKLATAIIGISSIHLLKTFINAHAYDEKTIIAQTAI
                     HFVFLVSAIAIALSDRIMNYGNSH"
     gene            complement(4845..5006)
                     /locus_tag="VFI43_06710"
     CDS             complement(4845..5006)
                     /locus_tag="VFI43_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET7061852.1"
                     /translation="MGTTCFAWMIKDGLARAETHYFSGNREEIREQAVGIALQGAIDL
                     LNSIPPEQA"
BASE COUNT         1048 a         1434 c         1302 g         1229 t
ORIGIN      
        1 tcgtaaaaga tttccgaaca tggcccacac ggccccgtgt ctcccatttg ccagaaattg
       61 tcctgcgtgg ggatgcgcgt gaaccgggca gggtcgatgc caatttcgtt cagccagata
      121 tcggctgctt cgtcgtcctc ggcatacact gtgacccata gtttttctgc cggtatatcg
      181 agcgtgacgg tcaggaattc ccaggcgaat ttgatggcgt ggcgcttgaa ataatccccg
      241 aagctgaaat tgcccagcat ctcgaaaaac gtgtgatgcc gcgccgtata gccgacgttt
      301 tccaggtcgt tgtgcttgcc gcctgcgcgc acacaacgct gcgaacttgt cgcgcggaca
      361 taaggccgct tctcatggcc gagaaataca tccttgaact gcaccatgcc tgcattggtg
      421 aacagcaagg tgggatcgtt gcctgggacg agcgagctgg atggcacaac cgcgtggccg
      481 tgcgattcaa agaattcaag gaacttctgc ctgatttcgc tgcttttcat atagggtttt
      541 aattgcccgg caagaggacc ggaaaccgaa agccttccag tcaacgccgc gaaatttcgg
      601 ttgccgccat cccaagggat tcgccattga cgatagcgag attttgaacc tttactttga
      661 ccgtaagttt ctcgtcggct ttaggtaagt cgccattggt cagaatcatg atttccccgc
      721 tattgtcctt cagcacatag gatttcatgt cgatcagcgg gatgcgcgtc tgatctttcg
      781 ccgtcccgtg cagcgtaacc tctttcccgt caaagttcgc gggagatttg gcaatgtcgc
      841 caacaggagt cgcgttaatg ccgcaaccac tgaatacaag tgcacttgca agagccgccg
      901 ctcgggcggc acggattggc ataaatgtac gcattgtctt ttttgtcatt attcgtcctc
      961 tttgttgcgc aacactcggg cgatgacggc actgctgaac ccccggccta gaaggaaacg
     1021 tgtctgtctc acaaactctt ttgcattgcc cggcatgacg ccgaactttt tgcgccatac
     1081 ttcctgcgcg gtgacctgct cagcttcctt gagattgggc aacgcctcgg cgattaaatt
     1141 ctccggaatc cctttttccc gcaattcata tgcgattcgc tggctgccga accggcgctt
     1201 gcgcgtatgt atgacttgtt ccacgacgcg ggcaggtgaa agccagccag cccgctcaag
     1261 ctcgtcaaga atggcggaaa gttcttccgg ggtgccggcg tggggcgcaa gctttttttc
     1321 aagctccagc cgggagtgtt cgcgccgggc aagatgagcc aaggcgcgat gtttcaagct
     1381 tggtccttgg cccgtggttc tggccttgga cgccgcggtt cgcatggggg ttcaggtcca
     1441 gttcacccga aggttaagct atagggcggc cgtacttgcg gtcagggaca gcccattatg
     1501 catgcacggc ttctgccgga ttggagacac ctactgctgc ccggatcttg gactctattt
     1561 ccatcgcaat gtcgtggtgt tccttgagga attcgcgagc gttatctttg ccctgcccga
     1621 ttttgtctcc cttataggca taccaggccc cggatttgtc gatcagcttg tgctgaaccc
     1681 caagctcgac aatttcccct tcgcgagaaa tcccctcgcc atagagaata tcgaattccg
     1741 cctgcttgaa gggaggggcg accttgtttt tcacgacttt gacgcgcgtt tcgcttccga
     1801 tcacctcttc acccttcttg atcgaacccg tgcggcggat atcgaggcgt accgatgcgt
     1861 agaatttcag cgcatttccg cccgtggtgg tttcagggct gccgaacatg acaccgattt
     1921 tcatgcgaat ctgattaatg aagatgacca tggtattact gcgcttgatg tttgcagtca
     1981 acttgcgcaa cgcctgggac atcaatcgcg cctgcaaacc catctgcggt tcgcccattt
     2041 ctccctcgat ttccgcccgc ggcgtcaaag cggcgacgga atccacgacc accacgtcaa
     2101 tcgaccccga gcgcaccagc atgtccgcga tttccagcgc ctgttcgcca ttgtcgggct
     2161 gggaaatcag caactcctga acgttgacgc cgattttttg cgcatactgc ggatcgaggg
     2221 cgtgctccgc atcaatgaac gccgcggtcc cgcccatttt ttgcatttgc ccgatgacct
     2281 gcagggtaag cgtcgttttg ccggatgatt ccggaccata gatttcaatg acccgtccac
     2341 gcggcaaccc gcctaccccc agcgcgatat cgagtccaag cgatccggtc gaaatcacct
     2401 caacgtcctt ggccacatca ctggcgccca gacgcatgat agagcccttg ccgaactgct
     2461 tctcgatctg ggaaagtgcc gccgcgagcg ctctgcttct attgtcgtcc atgaatattc
     2521 ctctaaaaag tgggcggatt atcgcatatg tggccacggg ctggaaggcg aaggaatgcc
     2581 ccttccgctt gatgatgaat taattgttgc gggaaaaacc ttcctctcct ggaacttgcg
     2641 aaggatacga ggaccggggt tacggatcgg atcagggcag gatataggtt ttgaaataca
     2701 gctctgcctt taccgggtcc agtccacgca gcgcagcgta taccttgcgc atctccgcgc
     2761 gctcacccgc gatggcatag tccaccccca ggctgtacag cgcgtccacg tttttaggct
     2821 gaatacgcac tgtctcacga taagcctcta ttgcatcacc gtataggtcc agttcatcgt
     2881 aagtttttcc gagctgatac caaaccgtcg cgttttcggg tttgaagcgc aaggactggt
     2941 cgtatgcgtg tatggcatga ggatacttct cggacttatg gtaggcattg ccgagttcat
     3001 accaggcttc ggcgttctcc ggttgcagac gtacggcatc ctgcaaggca cgagtggcat
     3061 gttcatattc tttcaagtgc atatacgcca ttccaagccc atgccacgct tccccataac
     3121 gcgcctgatg gcgtatcgct tggcgataag catgaaccgc ctgccggtat tgtttaagat
     3181 ggatggaagc agcgcccact tcgaaccagc cggctgcact gtcctgatcc cgctcgacat
     3241 gcagctgcga aagcttcaat aagccgcgcc agtcgccctt tttctcgagg gcaaagcagc
     3301 ggttcagcca ttccagccca ttttctttct tcgttgaggc aggagtgacc ttcttttcaa
     3361 gttcctttaa ccagtctacc ggtaatgcaa aagcgaggtt ttgcccttcg acgtggcgga
     3421 acgctagaat tccgatcagc tggcccgaat cactgaacaa tccccccccc tcaaaaccct
     3481 gggaaggaac agcggagacc tgcatatacc acgatccttc gtgcggaagc agcccggaga
     3541 ttttcgcgct atggatgacg ggctgaggct cctgtttcga ggatatgccg acagcgtaca
     3601 ctcgctcgcc ggcccagggt tttttgctgc cagccaggcc cacgggtcgg atacccagcc
     3661 cggcaacgtc gagctcgcag aggtcaagat cctgcctttt atggcgtacg accgccttga
     3721 acgccttgcc cgcctcccga atatgaatac ttgctccacc acggataagc gtgcaactgg
     3781 tgacgacctc accctccccg atcgcaacgg cactacctcc tgcaacgcgc tttcctctcg
     3841 cgtcgaaggt ttccaccgcc actactgaat cacgaacccc tgaaaaaacc tgttcgtcaa
     3901 cctttgctgt atccgctgga gcgagggacg aaagcagcgc tgacgcaaaa cctccaagca
     3961 gagccaggca tgaatgcctt tcaactcgtt ttgcgcatcg gttggaaaag ctgtcgtggt
     4021 gcatggccgg gtcgtagagt tattgttaat gattgtgcgc aacaaaaggt gggcaatgta
     4081 gaatgtcggc ttttttatgc gacacgaact ggattttatg gcctatggaa aataacgaga
     4141 attcctctgg ctctcggact cccgcatatt caggaagaat ccagcccatg gcgtatttca
     4201 tctttacgac gcgctggctg caattgccct tgtatctcgg actgattctt gcacaatgcg
     4261 tctacgtgta tcacttctgg gttgaactga ttgacctgat cggcgccgtg atgggcaatc
     4321 aggctgcact gcagcatata ctcgatgcgg tggctatcga gggtgctccc cggccctcga
     4381 aaatcaacga gactacgatc atgctggttg tgctgggcct gatcgacgtg gtgatgattt
     4441 cgaacctgct tatcatggtt attatcggag gttatgaaac ctttgtgtcc cgtatgaacc
     4501 tggaggggca tccggatcaa ccggaatggc tgtctcatgt gaacgcgtcg gtgctcaagg
     4561 tgaagctggc gacggccatc atcggcattt catccattca cctgctcaaa acctttatta
     4621 acgcgcacgc atatgacgag aaaacaatca tcgcccagac ggcgatccat ttcgttttcc
     4681 tggtttccgc cattgcaatc gccctgagcg accgcatcat gaattacggc aattcccatt
     4741 aagcttaggt catcccatcc ttttctgtcc aagatgtgca agcaatgatt gaaagggtgg
     4801 cgcagaggat tccggcattt catagtttcg ggataaggac gctgtcaggc ttgttccggt
     4861 ggaatactgt ttagcagatc gatcgcgcct tgcaacgcaa tacctaccgc ctgctctcgt
     4921 atctcctcgc ggttgccgga aaaatagtgc gtttcggccc gcgccaagcc gtcctttatc
     4981 atccaggcaa aacaggtcgt gcccaccggt ttc
//
LOCUS       DASSHJ010000174         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_438075, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000174 DASSHJ010000000
VERSION     DASSHJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438075"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFI43_06715"
     CDS             <1..991
                     /locus_tag="VFI43_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RodZ family helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7061853.1"
                     /translation="SGAGQATPGQILRAARLGRGLSIQDVARQLRLSVRQVTALEEDD
                     YSKLSSGTFLRGFVRNYAKLLQLDPAPLLQRIEEALPPPQTQTISYQIEGIPFPSGRN
                     RGKRAVIMGLAIVIALSLLIYEIYRGNETNVERREAVEKAPVSAEIKDQADTPASAPQ
                     SSLLSNLLEEAGETGVPQTEEENRPSLDAAEPAFIEKKTVRPPIAQAPAISPPSKPAL
                     VPVPGPTTAVPPKPAGGESPAAAAIPESADGSDVVRLSFEGDSWTEVKDGRGKLLLSR
                     INRSGTEQVLRGKSPFALTIGNAAEVKLVYNNKPVDLARYTNAYGGTARLSLE"
     gene            1039..2277
                     /gene="ispG"
                     /locus_tag="VFI43_06720"
                     /gene_synonym="gcpE"
     CDS             1039..2277
                     /gene="ispG"
                     /locus_tag="VFI43_06720"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET7061854.1"
                     /translation="MSQNFFPPRRNSVGVRVGTVTIGGGAPVVVQSMTNTDTEDELAT
                     TKQVEQLALAGSELVRITVNTVEAAKAVPGIRARLDDRGCDVPLIGDFHFNGHKLVTE
                     YPGCAKALSKYRINPGNVGHGRKRDEQFSILIETACKYDKPVRIGVNWGSLDPELLAR
                     MMDENARSADPRDASHVMHEALITSALQSAARAEEIGLGRDKIILSCKMSGVRDLISV
                     YRDLASRCDYALHLGLTEAGMGSKGIVASTAALSVLLLQGIGDTIRISLTPEPGGDRA
                     REVIVAQEILQTTGLRAFVPLVAACPGCGRTTSTYFQELAESIQGYVRERMLVWRDQY
                     DGVENMTLAVMGCVVNGPGESKHANIGISLPGSGERPVAPVFVDGQKSVTLKGDNIAG
                     EFRQIVDQYVQTKYPKKIAQ"
     gene            2274..3539
                     /gene="hisS"
                     /locus_tag="VFI43_06725"
     CDS             2274..3539
                     /gene="hisS"
                     /locus_tag="VFI43_06725"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET7061855.1"
                     /translation="MTTRTVQAVRGMNDILPDEVYLWEYFEQIVGEWMAGYGYSNIRM
                     PIVEQTDLFVRSIGAVTDIVEKEMYSFQDRLNGDSLTLRPEGTASCVRAVLEHNLLYG
                     GPQRLYYSGPMFRHERPQKGRYRQFYQVGAEALGYPGPDIDAELIIMCADLWKKLGIS
                     DIRLEIGTLGDAESRAAHRARLIAYLERHLGKLDDDASRRLHSNPLRILDSKNVEMRE
                     IIESAPRLLDDLDEESLVHFEGVQRILREQGIDFEVNPRLVRGLDYYNRTVFEWVTGR
                     LGAQGTVCAGGRYDGLVEQIGGKSSPACGFALGVERVLALMMESGEDIHPSVPDAYVI
                     HQGELADALAWKTVRHLRDGGLKAILHCGGGSFKSQMRKADASGARFAVIIGEDEARV
                     GEISIKPLREAAVQVRVGLAETVDLLKRA"
     gene            3561..4196
                     /locus_tag="VFI43_06730"
     CDS             3561..4196
                     /locus_tag="VFI43_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7061856.1"
                     /translation="MAVYDLEEQEKIDTLKAWWKANGTLVIILIAAFAFGIGGMQAWN
                     HYQKQKTEQAVELYDDVLKAQGSGDPKKTSDAARLLMEGFPSSGYASRAALISARVSF
                     ENGDLQTTKSRLQWVLDNSKEEELKDLVRFRLAGILLDEKKFGEALGLLESKHGESFD
                     GLYADLKGDILGAMGKAGEARAAYQVALDKIGVKGTYHSIIQMKLDSLPAS"
BASE COUNT         1084 a         1235 c         1452 g          952 t
ORIGIN      
        1 gtcaggcgcc ggacaagcaa ctcctggaca gatcctgcga gcggcgcgtc tgggccgtgg
       61 tttgagtatc caggatgttg cgcggcaatt gcggctgtcg gtaaggcaag tcaccgcgct
      121 ggaggaggac gattacagca agctctcaag cggcactttt ttgcgggggt ttgtgcgcaa
      181 ctacgccaag ctgttgcaac tcgatcctgc gcccttgctc cagagaatag aggaagccct
      241 gcccccgccg caaactcaga ccatttcgta tcaaatcgag ggaattccgt ttccctcagg
      301 gcggaatcgg ggaaagcggg ccgtgatcat gggtctggca atcgtgatcg ctttgtcgtt
      361 gctgatctac gaaatttatc gcggcaatga aacaaacgtg gaaaggcgcg aggccgtcga
      421 aaaggctcca gtgagcgccg agatcaagga tcaggctgat acgccggcaa gcgcgcccca
      481 atcctcgctg ttatcaaacc tccttgagga ggcaggggaa acgggtgttc cgcaaacaga
      541 agaggaaaac aggccgtcac tggatgcagc ggaacctgct tttattgaaa aaaaaacggt
      601 tcggccgccc atagcacaag ctccagcgat ttcgcctcct tccaaaccgg ccctggtgcc
      661 agtaccggga ccaacgactg ctgttccacc aaagccggca ggcggtgaat caccagccgc
      721 cgcggcaatt ccggaatccg ctgatgggag cgatgtggta cggttgtcgt ttgaaggcga
      781 ctcatggacg gaagtcaagg atggaagagg aaaactgctc ttatcccgga tcaaccgttc
      841 cggaaccgag caagtcctcc ggggcaagtc gcccttcgct ctcacgatcg gcaacgctgc
      901 ggaagtgaag ctggtctaca ataacaagcc ggtcgatctg gcgcgctata cgaacgccta
      961 tggcggaacg gcgcgccttt cgctcgaatg atttctgcat aatctcacct ttatcaaact
     1021 ctcattccac atatgactat gtcacagaat ttctttccgc cccggcggaa cagcgtgggc
     1081 gtccgcgtag gtacggttac tattggcggg ggcgcgcccg tagtggtgca gtcgatgacc
     1141 aataccgaca ccgaagatga actcgctacc acgaagcagg tggagcagct tgcgcttgca
     1201 ggatcggaac tagtccgcat tacggtaaac acagtcgaag cggccaaagc agtgccggga
     1261 atccgcgccc ggctcgacga caggggatgc gacgtccccc tgataggcga ttttcacttc
     1321 aacggccata aactggttac ggaatatcca ggttgcgcga aagcgctttc caagtaccgt
     1381 attaaccctg gaaatgtcgg ccacggcagg aaacgcgatg agcaattttc gattctgatc
     1441 gaaacagcgt gcaagtacga caagccggtg cgcatagggg tcaactgggg cagcctggac
     1501 cccgaattgc tggcgcgtat gatggatgag aacgcgcgct ccgctgaccc cagggatgcg
     1561 tcgcatgtga tgcatgaagc cctgattact tctgccttgc aaagcgccgc ccgtgccgag
     1621 gagattgggc tggggaggga caagataata ttgtcatgca agatgagcgg ggtgcgggat
     1681 ttgatctctg tttatcgcga tctggcctcc cggtgcgact atgcgctgca cttgggcctg
     1741 accgaggctg gcatgggctc caagggaatc gtcgcctcga ccgcggcgtt atccgtgctg
     1801 ctgttgcaag gcataggcga cacgattcgc atatccttga ctcccgaacc tggtggcgat
     1861 cgtgcgcggg aagtcatcgt cgcgcaggaa atcctgcaga caaccggatt acgcgctttc
     1921 gtgccgctcg ttgccgcctg cccagggtgc ggccgcacca ccagcacgta tttccaggaa
     1981 cttgccgaaa gcatacaagg ctatgttcgc gaacggatgc tggtgtggcg ggaccaatac
     2041 gacggcgtcg agaacatgac cctggccgtg atgggatgcg tggtcaatgg cccgggtgaa
     2101 agcaagcatg cgaacatcgg gatcagtctt ccaggatcgg gggaacgtcc ggttgccccg
     2161 gttttcgtgg atggtcaaaa atcggtaacg ctgaagggag acaacatcgc gggcgaattc
     2221 cgtcagattg tcgaccagta cgtgcagacg aagtacccca agaaaattgc tcaatgacaa
     2281 cacgaaccgt gcaggccgtc cgcgggatga acgatatcct gccggacgag gtttacctat
     2341 gggagtattt cgagcagatc gttggcgaat ggatggcagg atacggttac agcaatatcc
     2401 gtatgcctat tgtcgagcaa accgacttgt tcgtgcgctc gatcggcgcc gtcaccgaca
     2461 tcgtcgagaa agagatgtat tccttccagg acaggctgaa tggggacagc ttgaccctgc
     2521 ggcccgaggg aaccgcttcc tgcgtacgag ccgttctcga gcataacctg ctctatggag
     2581 gaccgcagag gctttattat tccggcccca tgtttaggca cgagcgcccg caaaaaggtc
     2641 gatatcggca attctaccag gtaggtgcgg aggctctggg atatccaggc ccggatatcg
     2701 acgcggagct gatcatcatg tgtgccgatc tgtggaaaaa gctcggcatt tccgatatac
     2761 ggcttgaaat aggcaccctt ggcgacgccg aatcacgcgc ggcacaccgg gcaagactca
     2821 ttgcctacct cgagagacac cttggcaagc tcgatgatga tgcaagccga aggttgcata
     2881 gcaatccgct ccggatactc gacagcaaga acgttgaaat gagagagatt atcgagagcg
     2941 ctccccggtt gctggatgat ctggacgaag aatctcttgt acatttcgag ggcgtgcagc
     3001 gaattctgcg ggaacaggga atcgattttg aagtcaatcc aaggttggtg agaggcctgg
     3061 attattacaa ccgtacggtg tttgagtggg ttacgggaag gctcggtgcg caaggtacgg
     3121 tttgcgcggg cggccgttat gacggcctgg tggaacagat cggcggaaag tcatcccctg
     3181 cctgtggttt tgcattgggg gtggaacggg tcctggcgct gatgatggaa agtggggagg
     3241 atatacatcc gtccgtcccg gacgcttacg ttatccacca gggcgagctg gcggatgcgc
     3301 ttgcctggaa aaccgtaagg catctgcgag atggaggctt gaaagcgatt cttcattgcg
     3361 gcggcggcag cttcaagtcg cagatgagaa aagccgatgc cagcggggct cgcttcgctg
     3421 tcatcatagg cgaggacgaa gcgcgagtgg gtgagatcag cataaagccc ttgcgggaag
     3481 cggcagtgca ggtcagggtt ggattagcgg aaacggtcga tttgctcaag cgggcataac
     3541 tggaattttc aggaaaagag atggcagtat acgatctgga agagcaggag aagatagata
     3601 cactgaaggc ctggtggaaa gccaacggca ccctggttat tattttaatt gccgcttttg
     3661 cctttggaat agggggtatg caggcatgga accactacca gaagcagaag accgagcagg
     3721 cagtggagct ctatgacgac gtacttaagg ctcaggggag cggcgaccct aaaaaaacga
     3781 gcgatgcagc gcgtttgctt atggaaggtt ttccatcgag cgggtatgcg tcacgggctg
     3841 ctcttatctc ggcgcgtgta agctttgaaa acggcgatct gcaaacgaca aaaagccgct
     3901 tgcagtgggt cctggataac agcaaggaag aagagttgaa agaccttgta cggttcaggt
     3961 tggccggtat cctgctggat gaaaaaaaat tcggcgaggc gctcggtttg cttgaaagca
     4021 agcacgggga gtcgttcgat ggcctttacg ctgatctcaa aggagatatt ctcggcgcaa
     4081 tgggcaaggc cggagaagca cgggccgcat atcaggttgc gctggacaag atcggcgtga
     4141 aagggaccta ccatagcatc atccaaatga agctggattc gctcccggct tcctaaggaa
     4201 cctctgaaca agtcctcatg gagcgcgttg ctggtaaaat cgggatgatc acaaggcgcg
     4261 cgacgcaggt cgtagcgggg tctacgatgc caaggagcgc aacgccgtgc gccccgaagc
     4321 gttgcccgag ggacctgcga agcgggatcg ggcagcggag gggatgcggc gaccgcggtt
     4381 atggattggt ggggcgtacc tcccgcccgg ggtcgcatct tcctgcggaa gaaactgctc
     4441 cgccctccgc ggtcgtacca ttcgcttcgt cgcctttcgc gcatcccatc gcgcaaagcc
     4501 gccgtcgcgc ccacgatgac ttattcagag gttcctaatg acccgtacag ttttgtcccc
     4561 actcataaac gtttccattc agctccgctg atcagaatgt tcccgcagtt tgctcgcacg
     4621 atccggcaca tcctgctact gaccgcttca ttcatgctgt ccggatgcgc ggggacggtg
     4681 gagatggcgc gcgaccttgt gaatgccgcc tcggacattg aca
//
LOCUS       DASSHJ010000175        10517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1094435,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000175 DASSHJ010000000
VERSION     DASSHJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094435"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFI43_06735"
     CDS             <1..562
                     /locus_tag="VFI43_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7061857.1"
                     /translation="AIMEATMSGPYERLSSRTESGLINIIIDTPKGSRNKFKYDESAQ
                     CFRLSRVLPAGASFPFDFGSIPKTLAEDGDPLDVLLISEAASFPGCLMAGKLIGTIFG
                     DQTEKNETVRNDRLLAVPVTPANPAPFSALAELPDAWLKEIEHFFVSYNQIEGRKYNP
                     IGRGGPQEADKALCAAEQRFTKQDNR"
     gene            559..1485
                     /locus_tag="VFI43_06740"
     CDS             559..1485
                     /locus_tag="VFI43_06740"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET7061858.1"
                     /translation="MKGQIRQFKPVNPVYGLLHKTIDEALEQLDATRPILDESIHNAR
                     KSMKNSRAELRLLREGMPGVAYQTENTRLRDAGRALSPIRDARSVIDAFNSLRNNFAK
                     ELEGVDVAPLEEILHSKLAKARSQLQLDMPDQSAQLQNCNHLLEDCLSLAKKEHFSSI
                     DSVVIHSGVQRIYRKGRNAFAQAKAERTTEVLHECRKQAKYLFNGLNVLVIATPNGNS
                     KALKHARRLAERLGDDHELAMLSEQISADDYVSVNAETIKVFRKLIEQRRAKLQNDSL
                     KVGEKLYQQKPREFTKKVMQDVVLPSRNLRQI"
     gene            1546..2337
                     /locus_tag="VFI43_06745"
     CDS             1546..2337
                     /locus_tag="VFI43_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7061859.1"
                     /translation="MGRPPSADESLEFSHHLADYARSLALDHFRTRFKISHKADKSVV
                     TVADRHIESELRSLIRERFPDHGIIGEEYGGTTRECSWILDPIDGTASFVLGNPLFGT
                     LIGSLRSQQPFTGLIEMPVMRERWTGDGKRALFYDSFGSHGVSVSDCKMLQHARLYIQ
                     IPDFLRSGHASRLYRLQKHVAASHSSCDCYAYGLLASGHCDLVIEDGLEPCDYLPIVP
                     VILGAGGFMSDWDGNPLDLNSGKAVVAAATQALLHEAIRALKSFR"
     gene            2478..3011
                     /locus_tag="VFI43_06750"
     CDS             2478..3011
                     /locus_tag="VFI43_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061860.1"
                     /translation="MTSSPLFTVHPCVMAVALIVLGTGAAHAQFTSSGSANEQIAQLG
                     PGQGVEVIGQPPGSMTSSIIGSPVPGQRESSLGLPSVLNQPAPGQERPGQEGLGSRPN
                     PGIGSSAFPMERNVVREKLNRTNREAADIDRNGRISSEEASRLPPGSLPPGVPLGVPL
                     SSGPQPPGNSRSSPLYR"
     gene            3488..7327
                     /locus_tag="VFI43_06755"
     CDS             3488..7327
                     /locus_tag="VFI43_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7061861.1"
                     /translation="MSAYKKVFLSYWQAGYEGADHINHKGQPICMNEATRHTEQAYND
                     YLLLKEFGIRTVRESVGWRSVEKNGHFDFSSVEPRARAAQKLGLQVIWTLCHYGWPDD
                     IDVYSPAFVDRFYRYCRRAVHFLESFSHSECASVYTPMNEISFTSWEFAKNRFMCSDV
                     RNDGGDELKRQLVRAALRGCQAIWQINPSARILHCDPLIHVVENPDRPELNDSAVSYR
                     ECQFTAWDMLSGKIEPELGGKPHFLDLMGINYYHSNQWEIQGDSLWWHLDHPRRAPFY
                     TLLNQVYQRYRRPVLLSETSHVGSGRGAWIKDVAQQLALAQQLGVDLRGVCIYPIVDR
                     PDWEEPHRWHKSGLWEVNLKEKEEKRYRRVISETYAMGLRDAQRLIQHVSHSFSTQVP
                     PITEGAPMQTIIVFSHLRWDFVYQRPQHLLARLAEHYKIVFIEEPMFHEHDSFLETLM
                     PAPNITVLKPHTPVSARGFHDEQLPHLIKLLRQFVNMDEAHIAWFYTPMALPLLQELD
                     PSLVIYDCMDELASFKNPPKQMLQRENALFKVADLVFTGGPSLYGAKRERHHNVHCFP
                     SSVDVVHFEQARDRNNRHPAHDDIPGPRLGYYGVIDERIDTDLIAKIADAHPNWQIVL
                     VGPVVKIDRASLPKRHNIHYLGQQPYKALPHFLAGWNVCLLPFALNESTRFISPTKTL
                     EYMAAELPIVSTPITDVVDLYGDVVAIGATPQAFIKACEDALLTTPEDYQQTSAKMRR
                     MISETSWNATAEKMHELMQQTSAQKDESLNQSQSRFQANALMRAEPQSTPSEYKQCVI
                     IGAGPTGLSAAYHLDKNTLLLDKNPVVGGWCRSIEDKGFTFDHAGHIMFSNDDYVQKL
                     YRILLGNNVHWQNREAWVYSKGVHTRYPFQGALYGLPPSVIKECIVGAMESRYGSLKK
                     PDSKQAAADCSITDGAPTPLSDCCADGSVPDAGKASVRQEQSGEPNKSENFENFIYRV
                     WGAGIAKHFAIPYNRKLWTVPLTEMETSWLGGRVPLPDLEEIIEGALQPVAKPMGPNA
                     RFGYPLRGGFQAMMSGFVPHIKGSIELEADVAKVSPEQHTITLRDGRRFRYDHLISTM
                     PLPELIRKIGNEAPQAIKDAAAALRHVSVRCVNIGVGRENISDKHWIYYPEDTIFHRI
                     FLQGNASPSCNPPGGFGLTCEISYSPAKPLPLDGQKLIDRCVEDCIKVGLLTREDKII
                     TTNLVDMPYAYVVYDHARARSVETAKKWLLEHDIILSGRYSEWEYYNSDHAFLAGKKA
                     ADLVKQLRPRQKISAAE"
     gene            7344..8147
                     /locus_tag="VFI43_06760"
     CDS             7344..8147
                     /locus_tag="VFI43_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7061862.1"
                     /translation="MIIDAHIHCSGEEKADDVLRALDWAEVDKAVLLAPFLTGNYSLR
                     DRESLQAANQYLAGLITHHKDRLIGFAVINPMLEGASDDLLRAHELGLTGLKMVPSGW
                     YPYDDCAHRIYETASGLNIPVLFHSGIFIDGKSGRFCRPVFFEAIRDHPGLRVTLAHL
                     GWPWTDEANAVGVIDLINGVPPDTSQFRFDISFGPPPVYRLDVLKKAIAVLTPALLQF
                     GSDVFLPCPGDLIKSRIADVSALLSDLDVDRPSRERIMGGTAAAWLGVD"
     gene            8149..9288
                     /locus_tag="VFI43_06765"
     CDS             8149..9288
                     /locus_tag="VFI43_06765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7061863.1"
                     /translation="MKIISTAERPINLLEIIGGSTIGGTETHVLQLIQNLPRDLFHVT
                     CLCVSESPFTSQLRDLGCDVHLTPMTDETDWQSIQLGASLVRASAIDVIHAHLPNAHL
                     LAGMLSRLTETPALATVHGRYLGIRDLEVHKLMHTHLCVVCKSAYFQGLTLGVPPSKL
                     RFIPNGVDTQLFRPGPRNGYLHSQLKIPSGAPLVGFVGRLSPEKGPSNFIKIASIAHN
                     KIRDCHFVLIGDGPLRQKIEEDIDSLGMEKFIHLAGLQTDMRAVYDSLDLVVSTSHSE
                     AMPLAILEAMASGLPVIATNVGGVIDLIEEGQTGLLNSADDFDGIARHIVLLMTEQSL
                     RMRMGASARKRIEKNFELRNIVSQTSELLKSLTQTGYSERSIEHL"
     gene            complement(9298..10269)
                     /locus_tag="VFI43_06770"
     CDS             complement(9298..10269)
                     /locus_tag="VFI43_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7061864.1"
                     /translation="MNLKQVWSMVKDTFASWLDDYAPSMGAALAYYTLFSIAPLLLIV
                     ISTAGLIFGQDAVRGEIFDQFRGLMGDQGAQAIQTLLASVSKPSAGIAGTIIGTALLV
                     IGATTVLGELQDDFDRIWRAPSKEKKGNSIWRWIHTRLLSIGIILGIGFLLIVSLVFS
                     AAMAALGKWWALRFSDLEAIITPINFIFSFALLSAVFALLYKSMPRVKISWGDVAAGA
                     IVTALLFSVGKLLIGMYIGKSAIGSGFGAAGSVVVLLVWVYYSAQIVLLGAEFTRVYS
                     QTHGSRKGLLAFEEPVDQTPPAPSKSRTAAMGDRKSESMASSGERLA"
BASE COUNT         2516 a         2897 c         2774 g         2330 t
ORIGIN      
        1 ggcaataatg gaggcaacga tgtcaggacc gtacgaacgc ctatcttcga gaacggaatc
       61 aggccttatt aatatcatta ttgatacgcc aaaaggcagc cgcaacaaat tcaaatatga
      121 cgagagcgca cagtgtttcc gtctgagccg cgtactgccg gcaggcgcgt ctttcccgtt
      181 cgattttgga tcaattccga aaacacttgc cgaggatgga gaccccctgg atgtcctttt
      241 aataagtgaa gcagcgagct ttcccggatg ccttatggcg ggaaaattga ttggcacgat
      301 tttcggggat cagacggaga agaacgagac cgtacgtaat gatcggcttc tggcggtgcc
      361 ggtcacgcct gcgaaccctg cgccgttttc cgcccttgcc gaactgccag atgcgtggtt
      421 aaaggaaatc gagcatttct tcgtctctta taatcagata gaaggacgca aatacaaccc
      481 aatcggccgt gggggcccgc aggaagcgga taaagcgttg tgcgcagccg agcagcgttt
      541 tacgaaacag gacaatcgat gaagggccaa atccggcagt ttaagcctgt caatcccgtc
      601 tatggtcttc tgcacaagac aattgacgag gcgctggagc agcttgacgc aactcgacct
      661 atcctggacg aaagtatcca taatgccagg aagtcgatga aaaactcgcg ggcagaactg
      721 aggctgttac gggaaggaat gccgggcgtg gcttatcaga ccgaaaacac gcgactgaga
      781 gatgcaggcc gcgctctttc ccccatacgg gatgcgagat cggttattga cgccttcaat
      841 tccctgcgca acaatttcgc caaagaactg gaaggagtgg acgtcgcccc gctcgaagaa
      901 atcctgcatt caaaactcgc gaaagcacgc agtcagcttc agctcgatat gcctgatcag
      961 tcagcccagc tgcaaaattg caatcacttg ctcgaagatt gcctttcact ggccaaaaaa
     1021 gagcatttct catctattga ttccgttgtc attcattccg gagtgcagcg tatctaccgg
     1081 aaaggccgaa acgcatttgc gcaggcaaag gcggagagaa cgactgaagt gctccatgag
     1141 tgcagaaagc aggcaaaata cctgttcaac ggcctcaatg tgctcgtgat agcaactccc
     1201 aatggcaatt caaaagccct gaagcacgcc cgtcgactgg ccgagcgact cggcgatgat
     1261 catgagttgg caatgctttc cgagcaaatt tctgcagacg actacgtgtc cgtaaacgcc
     1321 gagaccataa aagtcttccg taagctgatc gaacaacgac gagccaagct gcaaaacgat
     1381 tcgctcaaag taggcgagaa gctctaccag caaaaaccac gtgagtttac gaaaaaggtt
     1441 atgcaggacg tcgtacttcc ttctcgcaat ctccgacaga tatagaatga cagcgcaatc
     1501 gttctatctt ttggagtacc aaaggttgct ttaaaggggc gcagagtggg gaggccgccc
     1561 agcgccgacg aatccctgga attttcccat caccttgccg actacgcccg ctccctggcc
     1621 ctcgaccact tccgtactcg ctttaaaatt agccacaaag ctgataaaag cgtagtaact
     1681 gtcgcagacc ggcatattga gagtgaactg cgaagtctca tccgcgagag atttcctgac
     1741 catggaatta tcggggagga atatggtgga acaacccgcg aatgtagctg gatacttgat
     1801 ccaatcgacg gaacagcaag tttcgttctt gggaatcctc tcttcggaac cttgatcggc
     1861 tcgttacgct cgcaacagcc atttacaggt ttgatcgaaa tgccagtaat gcgggaacgc
     1921 tggacagggg acggaaaacg cgcgcttttt tatgacagct tcggctcgca tggggtatct
     1981 gtaagcgatt gcaaaatgct ccagcacgcc cggctctata tccagatacc tgatttctta
     2041 cgctctggac acgcaagcag gctttacagg ttgcagaaac atgttgccgc ttcccattcc
     2101 tcctgcgact gctatgccta cggcttgctt gcctcaggac actgcgatct cgtcattgaa
     2161 gacggcctcg aaccctgtga ttacctgccg atcgttcccg tgattctggg agctggaggg
     2221 tttatgtcgg attgggatgg gaatcccctg gacctgaatt cgggtaaggc tgtcgtagcc
     2281 gcagccaccc aagccctgtt gcatgaagcc attcgcgcat taaaaagctt ccgctgaccc
     2341 caacacttcc aacttttcct cgatgctgca agcggaacgt gcggtgtcgc actgaaaaac
     2401 gacaaatcga aagataggat caagccgaat cgagtttggt ttaatttgaa catggtctag
     2461 caaatgtggg ggttattatg acttcatctc ctttgttcac ggttcatccg tgtgtaatgg
     2521 cggtagcgtt gatcgttctg ggaacgggtg cggctcacgc gcaatttact tccagcggta
     2581 gcgcgaacga acagatcgcg cagcttggcc ccggtcaggg ggttgaggtt atcggccagc
     2641 cgccgggctc gatgaccagt tcgatcattg gaagccccgt acctgggcag agggagtcat
     2701 cgctcggcct accgtctgtt ctcaatcaac ctgcgcctgg gcaagagagg ccagggcaag
     2761 aggggctcgg ttcgcgacct aatcctggca taggctcgtc ggcttttccc atggaacgga
     2821 acgtcgtgcg ggaaaaactg aatcgtacca accgcgaggc cgcggatatc gaccgcaacg
     2881 ggagaatcag ttctgaagaa gcttccaggc tacccccggg atctctgcct cccggagttc
     2941 cactgggcgt tcccctgtcc agtggacctc agccccccgg aaattcacgg agcagtcctc
     3001 tgtaccgtta gcgaggcaga ctcggggcgc ccttgccatt acgcggttac gctttcaaac
     3061 tgtggccatc gcgttcatgg atttttgcac cctcgaaaac tcgagcggag ggcggcctcc
     3121 tgtttgcttc cgaagttctc tggggcagtt tataggagat gaggggattt ccaagccggg
     3181 ttagcccccg ttcgctgccg ctgcgagtgg cgcatttggc ttgaatgtcg gaaccctggg
     3241 gacatcctat ccgccttccc gctgattgct cttctctagt agcgcaattt tgaacccgcc
     3301 acatccgccc gcttcacgcc tgaaaagcgc tatcgcattc tcacaaattc cttcccgccc
     3361 ttgaaggtgc aattggctca atctgagaga gagccaaatg tacgttatcg cacagtccgc
     3421 cttactaaaa actcagatca tggcaaagct gggcggaacc cccgcgcttg attatggaat
     3481 ctataccatg agtgcatata aaaaagtctt tttatcctat tggcaagcag ggtatgaggg
     3541 agcggaccac atcaatcaca agggtcagcc catctgcatg aatgaggcta cccggcacac
     3601 cgagcaggcg tacaacgact atctgctctt gaaagagttc gggatccgga cggtgagaga
     3661 aagtgttggc tggcgatcag tcgaaaagaa cggacatttt gacttttcgt ctgtcgagcc
     3721 acgcgcccgg gcggcccaaa aacttggctt acaggtcatc tggacgctat gccattatgg
     3781 atggcctgat gacatcgacg tctactcccc tgcattcgtc gatcggtttt atcgatattg
     3841 ccgtcgggcc gtgcattttc tcgagtcgtt ttcgcatagc gaatgcgctt cggtttatac
     3901 gcccatgaac gagatttcgt tcacaagctg ggaattcgca aaaaaccggt ttatgtgctc
     3961 cgacgtcagg aacgacggag gcgatgaact taaacggcag ttggtgcgcg cggcgttgag
     4021 gggttgccag gcaatctggc aaatcaatcc gtcagcgcgc attctgcatt gtgacccgct
     4081 tattcatgtc gtggagaatc ccgatcggcc tgaactgaac gatagcgcgg tttcctatcg
     4141 ggaatgccag tttactgcgt gggatatgct ttctggaaag atcgagcctg aactgggtgg
     4201 gaaaccgcat tttctcgatc tgatgggaat caattattat cacagcaacc aatgggaaat
     4261 tcagggcgac agcctgtggt ggcatcttga ccatccacgc cgcgcgccct tctacaccct
     4321 cttgaaccag gtttatcagc gttaccgccg tcccgtcctg ttatccgaaa cgagccatgt
     4381 tggcagcggc cgcggcgcat ggataaaaga tgtggctcag cagctcgcac tggctcaaca
     4441 gcttggcgtc gatttacgcg gcgtgtgcat ctaccccatc gttgatcggc ccgactggga
     4501 ggaacctcat cgatggcaca aaagcgggtt atgggaagtc aaccttaaag aaaaagaaga
     4561 aaaacgttat cggcgggtca tttccgaaac ttatgcaatg ggcctgcgtg atgctcagcg
     4621 gctcatccag cacgtttcac attctttttc aactcaagta ccccctatca ctgaaggagc
     4681 tccaatgcag acaattattg ttttctcaca tcttcgctgg gatttcgtat atcagcggcc
     4741 acagcatctt ctggcgcggc tggctgagca ttacaagatt gttttcattg aagagccaat
     4801 gtttcatgag catgacagct ttcttgagac gttgatgccc gcacctaaca tcaccgtgct
     4861 caagccgcac acgccggtgt cggcccgcgg ttttcacgac gaacaattgc cgcatctgat
     4921 caagctcctg cgtcagtttg tcaacatgga cgaagcgcac atcgcatggt tttacactcc
     4981 gatggcacta cccctgttgc aggagctcga tccctcccta gtcatttatg actgcatgga
     5041 cgagctggcc agcttcaaaa acccgcccaa gcagatgctg caacgcgaaa acgccctgtt
     5101 taaagttgcc gaccttgtat ttaccggtgg cccgagcctg tatggcgcga agcgcgaacg
     5161 ccaccacaac gtgcattgct ttccgagcag cgtggatgtc gttcacttcg agcaggcacg
     5221 cgaccgcaat aacaggcacc cggcacacga cgatataccg ggacctcgac tcggctatta
     5281 tggcgtgata gatgagcgta tcgacacaga cctgatcgca aaaattgcgg acgcccaccc
     5341 aaactggcag atcgttctgg tcggtccggt ggtaaaaatc gaccgcgcct cccttccgaa
     5401 acggcacaat atccattatc tcggacagca gccatacaag gcgctgccgc acttcctggc
     5461 tgggtggaat gtttgcctgc tgccattcgc gttgaacgaa tcaacgcggt tcataagccc
     5521 caccaaaaca ctggagtaca tggcggcgga actgcccatc gtcagcacgc ccattaccga
     5581 tgtcgtggat ctctacggcg acgtcgtagc aatcggggct acgccgcagg cttttatcaa
     5641 agcctgcgaa gacgctttat tgacaacccc cgaagactat caacagacca gtgcgaagat
     5701 gcgacggatg atttcggaga cgtcgtggaa tgcgactgcg gagaaaatgc acgaactgat
     5761 gcagcagacg agcgcacaaa aggatgaatc gctcaatcag tcccagtcga ggttccaggc
     5821 caatgccctc atgcgagccg aaccccaatc gacgccctcc gaatataaac agtgcgtcat
     5881 catcggtgcc ggtcccaccg gtttgagcgc tgcatatcat ctcgacaaga ataccctttt
     5941 actggataag aatccggtcg tagggggatg gtgccgctcc attgaagaca aaggttttac
     6001 cttcgatcac gccggccata tcatgttttc aaacgacgat tacgtccaga agctgtacag
     6061 gatactgctg ggtaacaacg ttcactggca aaaccgcgaa gcctgggtat acagcaaggg
     6121 agtccacacc cgctatccat tccaaggggc gctttatggc ttgccgccca gtgtcatcaa
     6181 ggaatgtatc gtcggcgcga tggaatcgcg ttacggatca ctgaaaaagc cggattccaa
     6241 gcaggcagca gccgactgct cgatcaccga cggcgcgccc acacccttat ccgactgttg
     6301 cgctgacggg tcagtgcctg atgcgggtaa agcgagcgtc cgccaggaac aatccggcga
     6361 gccgaacaag tcggagaatt tcgagaattt tatttacagg gtgtggggtg cgggaatcgc
     6421 caagcacttc gcgataccct acaaccggaa actgtggacc gttccgctga cggagatgga
     6481 aacatcctgg ctcggggggc gtgttccgct acctgatctg gaagaaatca tcgaaggagc
     6541 tctccagcct gtcgctaaac ccatgggccc caacgcgcgc ttcggctatc ctctccgcgg
     6601 cggctttcag gccatgatgt ccggttttgt tccccatatc aagggatcca tcgaactgga
     6661 ggcggacgtg gcgaaggtat cacccgaaca gcatacgatc actcttcgtg acggccgccg
     6721 ctttcgctat gaccacctga tcagtacgat gccgctgccc gaactgatcc ggaagatcgg
     6781 taacgaagcg ccccaagcca tcaaggacgc ggccgctgct ctccgccatg tctccgtgag
     6841 gtgcgtgaat atcggggttg gaagggaaaa catttccgac aagcactgga tttattatcc
     6901 ggaggatacg atattccacc ggatctttct ccagggcaat gcgagcccgt cctgcaaccc
     6961 cccgggcgga ttcggcctca cctgcgagat atcctattcg cctgccaagc cacttcctct
     7021 tgacgggcag aagctgatcg accgatgcgt tgaagactgc ataaaggtcg gcttgctgac
     7081 tcgcgaagac aagataatca cgaccaacct cgtggatatg ccctacgcat acgtggtgta
     7141 tgaccacgcc cgggcgcgca gcgtggaaac cgccaagaag tggttgctgg agcacgatat
     7201 catcctttcg ggacgttaca gcgaatggga atactacaac tcggatcacg cattcctggc
     7261 cgggaagaaa gccgcagatc tggtcaagca attgagacca aggcaaaaaa tctcggcagc
     7321 ggaatgaatt ctctgttccg cagatgatca tcgacgccca catccactgc tctggagaag
     7381 aaaaggcaga cgatgtctta agagcactgg attgggcgga agtggataaa gcggtactcc
     7441 tggctccgtt cctgacaggg aattattcgt tacgggaccg ggagtccctg caagcggcaa
     7501 atcaatatct cgcgggactg attacgcatc ataaggatcg cctgatcggc ttcgcagtga
     7561 tcaatcccat gctcgaagga gcctcggatg atctgctccg tgcgcatgaa ctggggctta
     7621 ccggcctgaa aatggtacct tcaggctggt acccttatga tgattgcgcg catcgcatct
     7681 acgagaccgc atccggcctc aacattccag tgcttttcca tagcggaatt tttatcgacg
     7741 gaaaatcagg acggttctgt cgtccggtat tcttcgaagc gatacgcgac catcccggcc
     7801 tgcgcgtcac cctggcccac ctcggatggc cctggacgga cgaggcgaat gcagtgggcg
     7861 tgattgatct tatcaatggt gtgccacccg acacgagcca gttccgcttc gatatctcgt
     7921 ttgggccgcc gcctgtctac cgcctcgacg tattgaaaaa ggcgattgct gtcctgacac
     7981 ctgctctact tcagtttgga agcgatgtat ttttgccttg cccgggcgat ctgatcaaaa
     8041 gcaggattgc agatgtttcc gcgctgttga gcgatctcga cgtagaccgt cccagccgtg
     8101 aacgcatcat gggaggcacg gcagccgcct ggttaggagt cgactaagat gaaaataatt
     8161 tcgactgcgg aacgtccgat caacctgctg gagattatcg gggggtctac gatcggcggg
     8221 accgaaactc atgtattgca actgatccag aatcttcccc gggatctgtt ccatgtaacc
     8281 tgcctatgcg tttccgaaag tcctttcaca tcccaattgc gcgatctggg atgcgacgtg
     8341 catttaacgc ccatgaccga cgagacggac tggcagtcca ttcagctcgg cgcaagcctc
     8401 gttcgggcca gcgccatcga cgtcatccat gcgcaccttc ccaacgcgca cttgctggca
     8461 ggcatgctga gcagattgac cgagacacct gcattggcca ctgttcatgg tcgttatctg
     8521 gggataagag atcttgaagt ccataaattg atgcatacgc atctttgcgt agtctgcaag
     8581 tcagcctatt tccagggcct gacgcttggc gttccgccca gcaaactccg ctttatccca
     8641 aacggtgttg acacacagct gttccgcccc ggccctcgaa acggctatct gcactcccag
     8701 ctcaaaatcc cctctggtgc gccgctcgtt ggatttgtgg ggcgcctatc cccggaaaaa
     8761 gggccgtcaa actttattaa aattgccagt attgcccata ataaaataag ggactgccac
     8821 tttgtgctga ttggggatgg cccgctccgc caaaagattg aggaagacat cgacagcctc
     8881 gggatggaaa aatttattca cctggcggga cttcaaaccg acatgcgggc agtgtatgac
     8941 tcccttgatc ttgttgtttc cacttcacat tcagaggcca tgccgctcgc catcctcgaa
     9001 gcaatggcct cgggcctccc ggtcatcgca accaacgtag gcggagtgat cgacttgatc
     9061 gaggaggggc agacgggtct actgaacagc gctgatgact ttgacggcat tgcaagacat
     9121 atcgtcttgc tgatgactga acagtccctc agaatgcgga tgggcgccag cgcgcgaaaa
     9181 cggatagaaa agaactttga gctcaggaat attgtgagcc agacatccga attactgaag
     9241 tcgctcaccc agacggggta cagcgaacga agtatcgagc acctctaaaa acgctatcta
     9301 ggcaagacgt tcgcctgaac tggccattga ttcgcttttg cggtcgccca ttgctgccgt
     9361 acggctttta cttggggccg gaggggtttg atcaacaggt tcttcaaagg cgagcaaacc
     9421 ttttcgagac ccatgggttt gagagtacac ccgcgtaaac tcggctccga gaagaacgat
     9481 ttgcgcggaa tagtaaaccc ataccagcaa gaccacaacg gaaccggccg cgccaaagcc
     9541 agagcctatg gcgctctttc ctatatacat cccgataaga agcttgccta cagagaacag
     9601 cagcgctgtg acgatcgctc ctgccgcgac atctccccaa ctgattttga cccggggcat
     9661 cgatttgtaa agcagggcga acaccgcaga tagaagtgcg aagctgaaaa tgaagttgat
     9721 gggcgtgata atcgcttcca ggtctgagaa tcgcagcgcc caccactttc ccaatgcggc
     9781 catcgcggcg ctgaaaacga gcgacacaat gagcaggaac ccgatcccca gtatgattcc
     9841 aattgacagc aggcgggtat ggatccaccg ccatatgctg tttccttttt tctccttgct
     9901 cggagcgcgc cagatccgat caaagtcatc ctggagctcg cccaagacgg ttgtcgcgcc
     9961 gatcacgagc aatgcagttc caataatcgt tccggctatg cccgctgacg gcttgctgac
    10021 acttgcaagc aacgtttgta ttgcctgagc cccttgatcc cccatcaggc cccggaactg
    10081 atcgaaaatc tctccgcgta ccgcatcctg accgaaaatg agcccggcgg tcgagatgac
    10141 gatcaacaaa agcggcgcga tggagaacag agtgtagtag gcaagcgccg ctcccatgct
    10201 aggagcataa tcatcgagcc atgacgcgaa agtatccttg accatggacc agacctgttt
    10261 gagattcata tggacctcct ttttcaacgt ttggcttagt ccctaacgct tcttcatatg
    10321 caatcatagt gaaaacgcgt tgaacggtat gtacgtcgta ccaccgaatt cgtaaaacct
    10381 cggtacagcc gttgcttgag cctatttgcc tttctcgggt tggaagagga tgagattaat
    10441 gctacgaacg gatgacttta gggttcttcg gattcattgc gctgcaccga tccggagtct
    10501 tggtttgaat atctggt
//
LOCUS       DASSHJ010000176         6728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_583177, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000176 DASSHJ010000000
VERSION     DASSHJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583177"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="pdxA"
                     /locus_tag="VFI43_06775"
     CDS             complement(<1..915)
                     /gene="pdxA"
                     /locus_tag="VFI43_06775"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7061865.1"
                     /translation="MSKRPVIALTPGDPAGIGPDLCVQIAQLDLPCNLVVIADRGLLR
                     ERARLLRLPLELVDYPPFPEASGSPQVLHVLHMPLSRPAIPGKLDPSNATYVLNTLKR
                     AVEGCSNGAFSALVTGPVHKGIINDAGIPFTGHTEYLAERTNNKPVMMLAGGGMRVTL
                     ATTHLPLKDVPAAITQSLLEQKLKIIHRDLIAWFRVPNPRIAVAGLNPHAGESGHLGR
                     EEIDIIIPTLDGLRAEGMDLVGPLPADTLFNPSKLKEYDCIFAMYHDQGLPVLKHASF
                     GTAVNVTLGLPIIRTSVDHGTALELAGTG"
     gene            complement(953..2281)
                     /locus_tag="VFI43_06780"
     CDS             complement(953..2281)
                     /locus_tag="VFI43_06780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7061866.1"
                     /translation="MSKKFLERFIVLLAVMTAGTASAQQIASTSGIETIDHIVAVVNE
                     TVVTRHELDEVLKATVRQLQKQGVQPPASSVLEKQLLERIILNQVQLQLAKETGLTVS
                     DTELDQTLRRIAQQNNMSLPEFYSALERDGISFNRFRDEIRDEIILVRLKEREVSNRV
                     NVTEGEVDNFLNSQEGSPNQDEEYRISHILIQMPEKATPSQVSEKRLRADAALAQLKD
                     GAEFARVAAEFSEAPDAMEGGLLDWRPGAQLTKKFAEILGPMKPGEITPVIQTQAGLH
                     ILKLVDRRGKKESAAPTMVNQTHARHILIKISELTSEADARRRILELKERLDHGGNKF
                     EELAKLHSEDTSAPSGGDLGWVSPGDTVPEFEQAMATLKPGQTSDPVQTPFGWHLIQV
                     IEHRTQDVSKERQRQSARQAIRSRKADAAFQEWLQRLRDRAYVEYRLEES"
     gene            complement(2317..5106)
                     /gene="lptD"
                     /locus_tag="VFI43_06785"
     CDS             complement(2317..5106)
                     /gene="lptD"
                     /locus_tag="VFI43_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HET7061867.1"
                     /translation="MKSRFSRPACWSVLLFCLACDARAETPSPDSKSKYERTAPPGGK
                     NANGNKPAIIDNQPIPGAHEYETEAQGETGLRGTTSATSAQERQPSSPEGVRTAPQNY
                     TSPAVKSAPAEGISAGDTVHKETPPLAGGSEKVEEHALHEGEANLRTTTRPRTGVAAA
                     APQESGAAKPPGEQTAVEQYAVTKTDQIIGRFEQESDFKLRLNGRAEPEAAPRLATPR
                     PEAEPTKTKPVFVAAERLQGHPGKDIEAIGKAELASGEQFITADRMKYYETTEDAEAE
                     GGVRLEKQGDILEGSRLKFNLASKTGELDQPTYRLKDASSRGYADKVLFEGENRYRLQ
                     GATYTTCPAGDDSWLLQVADMEVDNSKKVGTAKGVKLTFKDVPILYTPWMNFAYSGER
                     KSGLLAPLYGTSVRTGLELTVPFYWNIAPNYDATISARMMSKRGVAINNEFRYLTNRS
                     SGVALLDILPYDLATKESRWRTSFWHDQNLTHGFWTRLDYNRVSDDAYFRDLGVNLNL
                     TSRTNLLQQAIVGYGRNLGDEGTLNVTAMGQQFQTIQDPLASIVKPYKRLPQFTMTVN
                     KPGVLGADLAFFGSWSNFSHPTLVSGQRVVLFPSVSYPIRRAFGYITPKVGIHHTRYS
                     LEDGTPDASPTRTLPIFSVDSGLAFDRNTSFRGEQFTQTLEPRIFYVYVPFRDQSQLP
                     NFDSAKTDFSFAQMLTENRFSGSDRINDANQVTVALTSRFIQSSTGIERLRLAVGQQI
                     SFIDRQITLDQPNTIPRRPDVIAAVSGFLTPTISTDASMQFDHQRFTGEVVRSGLSYR
                     PEPGKVLNVGYRFTRDVLHQVDASSQWRWGEKWQTVARINYSLQDHRILEGLAGLEYN
                     SCCWSLRFVLQHLITATQRSTTAAFVQLELNGLMQIGSNPLQVLQRSIPGYTKTGSQG
                     SSVVEGPQSGEHRLQ"
     gene            5446..>6728
                     /gene="mpl"
                     /locus_tag="VFI43_06790"
     CDS             5446..>6728
                     /gene="mpl"
                     /locus_tag="VFI43_06790"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HET7061868.1"
                     /translation="MHIHILGICGTFMGGIAVIASEAGHKVTGCDAAVYPPMSEQLRS
                     LGIDLIEGYSSEQTQLNPDLYVIGNVVSRGNPLMEEVLNCNLPYTSGPQWLAENILRN
                     KWVMAVAGTHGKTTTSSMLAWILDYAGMKPGFLIGGIPGNFGLSARLGKTPFFVIEAD
                     EYDTAFFDKRSKFIHFRPRTVILNNLEFDHADIFADLAAIEQQFHYLIRTIPGNGLIV
                     CNGREESLERVLCRGHWTSVEKIGVGGGWEAETTTDHPLEILFRGERLGALNWSLLGE
                     HNRMNGLAAIAAARHAGVPGSVALEALGQFRNVKRRMEVRGEVRKVTVYDDFAHHPTA
                     IRTTLEGVRGKVKGARIIAVLEPRSNTMKMGVWKDSLGDSLSEADLVFCYSASLGWDV
                     RSAMEPLGSKALIHDNLETLVASIALAARPHDHIL"
BASE COUNT         1368 a         1820 c         1940 g         1600 t
ORIGIN      
        1 accggttcct gccagttcca gcgccgtgcc atgatctaca gaggtgcgaa tgataggcaa
       61 gcctagggta acattcacag ctgtgccgaa gcttgcatgt ttcagcaccg gaaggccttg
      121 gtcgtggtac atcgcgaaaa tgcagtcgta ctcctttagc ttggaaggat tgaatagcgt
      181 gtcggcaggc agggggccga ccagatccat tccctcggcg cgtagcccgt cgagggtcgg
      241 gataatgata tcgatctctt ctcttccaag atggcccgat tccccggcgt gggggttgag
      301 tccggcaacg gcaatgcgtg gattgggaac tcgaaaccat gcgatcaaat cgcggtgaat
      361 gatcttgagc ttttgttcca gcagagactg ggtaatagcg gccggaacat ccttgagcgg
      421 caggtgggta gtagcgagcg tgactctcat cccacctccg gcgagcatca tcactggctt
      481 attattcgtt cgttccgcca ggtattccgt gtggccagtg aacggtatcc ctgcatcatt
      541 aataatgcct ttatgtaccg gtccggtcac cagagcgctg aacgctccgt tgctacaacc
      601 ttcaacagcg cgctttaaag tattgaggac gtacgtggcg ttggagggat caagctttcc
      661 cggaattgcc ggcctggaga gcggcatatg cagcacatgc aagacctggg ggctgcctga
      721 tgcttccgga aatgggggat agtccaccag ttcaagcgga agccgcagca aacgggcccg
      781 ttcccgcagc aatccgcggt ctgcgatgac aaccagattg catggcagat cgagttgggc
      841 gatctgcacg cacagatcgg gccctatgcc ggcggggtcg ccgggagtga gagcaattac
      901 cggcctttta ctcagtggga gacgtgattc tttattgtga gaaccgctaa tctcagcttt
      961 cctcaagccg atattcgacg tacgcgcggt cgcgcaaccg ttgcagccat tcctggaagg
     1021 ccgcgtccgc cttccgggaa cgaatcgcct gccgtgctga ctggcgctgg cgttccttgc
     1081 tcacatcctg ggtacggtgt tcgatgacct ggatcaggtg ccagccaaat ggcgtttgaa
     1141 cgggatcgct ggtttggcca ggtttgagcg tagccatcgc ctgttcgaat tccggaactg
     1201 tatcgccagg ggaaacccat ccaagatcgc cgccggaagg ggcgctggtg tcttccgagt
     1261 gaagcttggc caattcctcg aatttgttgc ccccatgatc cagtcgctcc ttgagttcga
     1321 ggatgcgccg ccgcgcgtcg gcttcggatg tcagttcgct tatcttgata agaatgtgac
     1381 gagcatgcgt ctggtttacc atggttggcg ccgcactctc tttctttccc cggcggtcga
     1441 caagcttgag gatatggaga ccagcctggg tctgaatgac aggcgtgatt tctcctggct
     1501 tcatcggtcc caatatctcc gcaaattttt tagtcagctg agcaccggga cgccagtcca
     1561 ggagtcctcc ttccatcgca tccggtgctt ccgagaattc agcggctacc cgcgcaaact
     1621 ctgccccatc ttttaattgg gcgagggcag cgtctgcccg aagccttttt tccgagactt
     1681 gcgagggcgt cgccttctcc ggcatttgaa tcaggatatg ggaaatcctg tactcttcat
     1741 cctggttcgg tgaaccctcc tggctgttga ggaagttgtc cacttcccct tcagttacat
     1801 tcacccgatt gctgacttcg cgttccttca ggcgtacgag aataatttcg tcgcggatct
     1861 cgtccctgaa cctgttgaaa ctgattccat cacgttccag cgcgctgtag aactcgggta
     1921 atgacatgtt gttctgctgg gcaatgcggc gcagagtttg atcaagctcc gtatcgctta
     1981 ctgtgaggcc ggtttctttc gccagctgca actggacctg attgagaata atgcgctcca
     2041 gcaactgctt ttcgagcacc gaggaagcgg gcggctgcac cccctgcttc tgcagttgcc
     2101 tcaccgtggc ctttagcacc tcatcgagtt catgccgggt gacaacggtt tcgttcacaa
     2161 ccgcgacgat atggtctatg gtctcgatgc cgctggtgga ggcgatttgc tgggcgctcg
     2221 cagtccccgc ggtcatcacc gcgagcagga caataaaccg ctccagaaat ttcttgctca
     2281 tgcacatcag cattagcaga gattcatggg aataaattat tgcagcctgt gttcgccgct
     2341 ttgcgggcct tcgacaacac tgctgccctg gctgccggtt ttggtatagc cgggtatact
     2401 gcgctgcaat acttgcaacg gattcgatcc gatctgcatc aagccattga gttcaagttg
     2461 tacgaatgcc gccgtggtcg atctctgggt ggcggtgata agatgctgga gcacgaatct
     2521 cagcgaccag cagcaggaat tatactcaag tcctgccaat ccttccagaa ttctatggtc
     2581 ctgcaaagag taatttatgc gcgccacggt ctgccatttc tccccccagc gccattgact
     2641 cgacgcgtcg acctggtgca gaacatcccg agtgaagcga tagccaacat taagtacttt
     2701 gccgggctcg ggccggtaac tcaggcccga ccgtacaact tcaccggtaa acctttgatg
     2761 gtcaaactgc atgctggcat ccgtgctgat cgtcggcgtc aaaaaccctg aaactgcagc
     2821 gatgacatcc ggccgcctag gaatggtatt gggctgatcc agtgttatct ggcggtcgat
     2881 gaagcttatc tgttggccca cggcaagacg caggcgctca atgcccgtgc tggactggat
     2941 gaagcgggag gtgagcgcca ccgtgacctg gttcgcgtca ttgatacgat cgcttccgct
     3001 aaaccggttt tcggtcagca tctgggcgaa gctgaagtca gtcttggccg agtcgaaatt
     3061 gggcaactga ctctggtccc ggaagggtac gtaaacgtag aagatgcgcg gctcaagagt
     3121 ctgggtgaac tgttctcccc ggaaagaggt gttgcggtcg aatgcaaggc cgctatccac
     3181 gctgaagatc ggcaaagtcc gggtcgggct tgcatccggg gtaccgtctt ccaggctata
     3241 ccgggtgtga tgtatcccca ccttgggtgt gatgtaacca aatgcgcggc gaatcggata
     3301 actgacgcta gggaagagta cgactcgctg gccactgacc aatgtaggat gggaaaaatt
     3361 gctccagctc ccgaagaaag ccagatctgc ccccaatacc ccgggtttgt ttacagtcat
     3421 tgtaaattga ggcagacgct tgtagggctt cacaatcgat gccagcggat cctggattgt
     3481 ctggaattgc tggcccatcg cggtaacatt caaggttccc tcgtctccca ggttgcggcc
     3541 ataaccaacg atcgcctgtt gtagcaggtt ggttcgagat gtgagattca ggttgacgcc
     3601 caggtcgcgg aagtaggcgt catccgagac gcggttgtaa tcaaggcggg tccagaagcc
     3661 atgggtcagg ttctgatcat gccagaatga cgtgcgccag cggctttcct tggtagccag
     3721 gtcatagggc aatatatcga gaagcgcaac gccactcgat cggttggtca gataacgaaa
     3781 ctcgttattt atcgcgacgc cgcgtttgga catcatcctt gcagaaattg ttgcgtcata
     3841 atttggcgcg atgttccagt agaagggcac ggtaagctcc agcccggttc tgacgctggt
     3901 gccgtacaga ggcgcgagca gcccggattt tcgctccccg ctataggcaa aattcatcca
     3961 cggggtatag agaatcggga catccttgaa cgtgagcttg acgcccttgg ccgtacccac
     4021 cttcttcgaa ttgtcgactt ccatatctgc gacctgcagg agccaggaat catccccggc
     4081 cggacatgtc gtgtaggtcg cgccctggag gcggtacctg ttctctccct cgaatagcac
     4141 tttatccgca tacccgcggc tgctggcatc cttgagccgg taggtgggct gatccagttc
     4201 cccggttttg ctggccagat tgaacttgag gcgcgagccc tccagtatgt cgccctgctt
     4261 ttccaggcgc acgccgcctt cggcttccgc atcctcggtt gtttcgtaat acttcatgcg
     4321 atcggcggta atgaactgtt cgccgctcgc cagctccgcc tttcctatcg cctcgatgtc
     4381 ttttccggga tgcccctgca aacgctccgc agctacgaat accggcttcg ttttggtggg
     4441 ctcggcctcc gggcggggtg tggccagccg aggggccgct tccggctccg ccctcccatt
     4501 cagtcgcagc ttgaagtcgc tctcctgctc gaaacgccca atgatctggt ccgttttggt
     4561 aaccgcgtac tgctcgacag cggtttgctc gcctggaggt tttgctgcgc ctgattcttg
     4621 cggagcagcc gccgccactc ccgtgcgtgg gcgtgtcgtg gtgcgcaggt tagcttctcc
     4681 ttcgtgcaga gcatgttcct ccaccttttc actcccccct gcgagaggtg gagtctcttt
     4741 gtggacagta tcgccagcgg aaattccctc tgcaggtgca ctttttaccg ctggggatgt
     4801 atagttctgc ggtgccgtcc gaacaccttc cggggaactc ggctggcgtt cctgggcaga
     4861 ggtagcggaa gtagtcccgc gcagcccggt ttcgccttgc gcctcggttt catactcatg
     4921 cgcgcccggt atcggctgat tgtcaatgat cgcaggcttg ttgccgttgg cattcttccc
     4981 gccaggcggg gccgtgcgct catatttgct tttcgagtca ggcgaaggtg tttccgcacg
     5041 cgcatcgcat gccaggcaaa acaacagaac tgaccagcag gcagggcggg aaaagcgtga
     5101 tttcatgtaa tggtattcat ccccacgggc ctgtcgctat ggatgaatcg ccgttttcac
     5161 agtcagattt aataatctcg catcccagct atcggagcat gagatcgctc gcggtagtga
     5221 aattggtctt gtgaaaacgg agctgccctg aggtggagct aaccggttag ctgagtcgac
     5281 gccggaattg acgagcggaa aacaaaagcc gtaagtgtaa caaaaaaact ggcgagacca
     5341 cggttatcct tgaggtttct ttaagccgca tataatccag ccccctgaat atccttgctc
     5401 ttgctatacg gtaattatta acagctttaa ttgggggaca tgaatttgca cattcatatt
     5461 cttggcatct gcggtacttt catgggaggt atcgccgtaa tcgccagtga ggcgggtcac
     5521 aaggtgaccg gatgcgacgc agcggtttac ccgccaatga gtgagcagtt gagatccctg
     5581 ggaatcgatc tgatcgaggg ttactcttcg gagcaaaccc agctgaatcc tgatctgtat
     5641 gtgataggta acgtcgtaag ccgtggcaac cctttgatgg aggaggttct caactgtaac
     5701 ctcccctata cttcgggtcc tcagtggttg gcggagaata tattgcgaaa caagtgggtg
     5761 atggcggtag cggggaccca cggcaagacg acgacaagct ccatgctcgc atggatactg
     5821 gattacgcgg ggatgaagcc gggcttcctg attggcggaa tacctggcaa tttcgggctg
     5881 tcggccaggc tgggaaagac tccctttttt gttatcgagg cggatgaata tgacaccgcc
     5941 tttttcgata agcgctccaa gtttattcat ttccgcccca ggaccgtaat acttaataat
     6001 ctcgaattcg atcacgcgga tatttttgcg gatcttgcag cgattgagca gcagtttcac
     6061 tacctgataa ggacgattcc gggaaacggg cttattgtat gtaacggccg ggaggagagc
     6121 ctcgaacgtg ttctgtgcag agggcactgg acctctgttg aaaagattgg ggtaggaggc
     6181 ggttgggagg ctgaaacaac aacggatcat cctctggaga tccttttcag aggggagcgg
     6241 ttgggcgcct tgaactggag cttgcttgga gaacacaacc ggatgaacgg acttgccgct
     6301 attgccgccg cccgccatgc gggggtgcct gggagcgttg cacttgaagc attggggcag
     6361 ttcaggaacg tgaagcggcg catggaggtg cgcggcgagg tgagaaaggt cacggtttac
     6421 gatgatttcg cccaccatcc cactgcaatc cgcacaactc tggaaggggt gcgcggcaag
     6481 gtgaaagggg cacgcattat cgccgtgctg gaacctcgtt cgaatacgat gaaaatgggc
     6541 gtctggaagg atagcctggg agacagcctt tccgaggccg atcttgtatt ctgctattcg
     6601 gcgagcctgg gctgggacgt aaggagcgcg atggagccgc tcgggtcaaa agcgcttatc
     6661 catgataacc tggaaacttt agttgcctct attgccttgg ccgcgcgccc gcacgaccac
     6721 atactcat
//
LOCUS       DASSHJ010000177        33161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_62787, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000177 DASSHJ010000000
VERSION     DASSHJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 33161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33161
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62787"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..3027)
                     /locus_tag="VFI43_06795"
     CDS             complement(208..3027)
                     /locus_tag="VFI43_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET7061869.1"
                     /translation="MSNIFHGQKAIASISAALTLFAAALVSSPPAQAAAHNISLSAEV
                     LPNGQVGYKRNGEDKAVIPGPTLFVKQGDTVTLSVTNNTGKPVGLRVPSLKNGSAAKA
                     APGGTVSFSFTASKTGAHAYLGDGQELLGLFGAVVVDGTDGKVESYVNGDGVITSVKA
                     ADLAKQYVLFMVGSTFWGAEISEAGTQTPLWTNPTFGAVEGDIVRFHVLSAGPGHTFH
                     LHAHRWLEQGAKSTIDVRLLANGSDSHTFTVKAGTGVGPGHWQYHCHVFSHMEAGMHG
                     SFRVDAANGSHRGPGNSIAGASPYGAIYGSSSKDPGLVTFVISDEPGSWFRSARSETI
                     FNITKGTRSLEVISPGSSVNFIMAETNTVHSMTSLLWPSAAGLPSSGHHSNGHHSNEG
                     KGKRRGQGAANIEHASMPFDQSRAYKGGGIVKLTTPGLYVFTCKVHPYMFGAVIVDDP
                     ATDGLDLGETINLATGIVNLPTSSDLATRLLSIFFIATAPNNWQNPASSAHWNITYPN
                     VPVRITGGATVNLSDVLVARYGNNIALTPPIPPAIQGVGEVWVDTQFEKTAGKTKPGT
                     ITVIDTSTWKAVRKIALPQINMNNPHNMWTNRDQSIVFQTQWFDNKMTMINQKTGALI
                     KNIQVGEAPAHVMTRPDTDDITVTNNGEDGISMIPVGKTEVVRMMPTQKSGQPATNPH
                     GHWISVEGGEALVVTPNIFTNDVGFYNLNGNGSILERTPVGESAPGAHPIAISMMPDG
                     SKFYAANLLHHNVSVLSKNGTLLKTINLIADYNPIDGTIADRDGDGQATVGMLPIQLP
                     VSPDGKAVVVANMGGTITVIDTATDKIAKMLPCDPGCHGANFGAKKGGGYYAYVTSKF
                     SNELVVVDVDPNNDGNVSDAVVAGRISLVSNHSTARDDTTTSLEGYGGQGVLGIPNVY
                     NTWVRHLPPEWKDQLTPAQQTASRH"
     gene            complement(3055..4077)
                     /locus_tag="VFI43_06800"
     CDS             complement(3055..4077)
                     /locus_tag="VFI43_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7061870.1"
                     /translation="MKCQHHSDVSKFFASSKPPFFAGSPQLKSFHTKLIIPFALLLGS
                     FLSAHAVAADHRIEMIAEQTADGLLAYQMIEHLVDGENVTSRYSPGPTIPGPTIVLTE
                     GDKVRLTIRNGITVTPDQQVSVHVHGVHYKILSDGSLKVINRIEDEGAYPDHGGRFYT
                     YLWDVAPGTAGTWPYHDHNYETHNGSEDRGLYGAIIVNPASGSVPAAHGENITSVPVR
                     SIKKDFVLYLSDDSFWGMEIDGHSHKQKALGDNPHLTAGRGDDVRFHIIAMGTYLNRF
                     QLRDYHWIDPGTSNLIREKNIGPLEKHVFTIKALNSARYVNANFSRKLMGVGGSFSVT
                     AAHHGH"
     gene            complement(4441..5139)
                     /locus_tag="VFI43_06805"
     CDS             complement(4441..5139)
                     /locus_tag="VFI43_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7061871.1"
                     /translation="MSIRILLVNSHQIVLWGLEKLLNAESPRMEIVGKASNRPDALRL
                     SRETDPDVVLLGLHLGKERGVDVISDLLRERQINIIIYTEAHNQADVDKAVLSGARGV
                     VYKEEPTQTIVKAIEKVHAGELWLDRCTIGRVFVDFVRAGQKPADLITKKITTLTRKE
                     RTIVCVFANEAGASNKKIAQKLCISEQTLRNHLTSIFSKLEITNRFDLFMFAKVHQHL
                     ISDPDLLPARSNGG"
     gene            5458..5892
                     /gene="gspG"
                     /locus_tag="VFI43_06810"
     CDS             5458..5892
                     /gene="gspG"
                     /locus_tag="VFI43_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET7061872.1"
                     /translation="MKYEHINNRSKAESGFTLLELLVVMVIIGLLAGYVAPKYFSQVG
                     KSEVKVAQAQLDSLGKALDQYRLDTGHYPATEQGLGALMTRPTNEPRWQGPYLKKAAP
                     PDPWGRPYAYKYPGEHSEYDLYSYGKDGQPGGNGEAADITNW"
     gene            5899..7083
                     /locus_tag="VFI43_06815"
     CDS             5899..7083
                     /locus_tag="VFI43_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7061873.1"
                     /translation="MRYEVKAVLARRGTVLLQLDAQSEEEARLQVTAQGGRVLNVRRR
                     FSGWASKQKVRFPLAHFSHELLSLLVAGLSLVEAIETLADKEQDVGVRQIIQGLLARL
                     YEGVTLSKALESFPQAFPPLYVATARASERTGDLPEALTRFITYQTQMDLVRKKLIGA
                     SIYPVLLLTIGLLVALFLLVFVVPKFSAIYEDAGGDLPWLSMLLIRWGHLVQEHGWQL
                     AGMVGVLLIIIIYGVQQPVFRTWASAQLWKIPAVGERMKVYQLARFYRTLGMLLRGGI
                     PVVKALEMVSGLLQAHFRPRVHAAAALIREGKTISSAMQAEGLTTPVGSRMLRVGERT
                     GHMGEMMERIGSFHDEEIARWVEWATKLIEPLLMAIIGGVIGGIIILMYMPIFQLAGS
                     VG"
     gene            7128..8780
                     /locus_tag="VFI43_06820"
     CDS             7128..8780
                     /locus_tag="VFI43_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET7061874.1"
                     /translation="MDVDQLLEIRRAASAREVPVIRYLEEVSGWTPDRTLSELGRLLR
                     MPALTMEGLRSLETAFEKLPFNEAITQECALFRSGEQYVLAVGNPFLPNLRAWADDRI
                     DVPAAWHLVHPSDLAAFFTKQEQSMRAMDSVLPGAQQRSQQVGEENLSIKSINEGTSQ
                     VVRLVHSTLYDAHKSQASDIHLEMVTGALSIKYRIDGVLSSIGVVQGADLAEQVISRI
                     KVMSDLDIAERRVPQDGRFKISIQGREIDFRVSIMPSSLGEDAVLRILDRQALADHVE
                     GLKLDHLGFDAPTMSSLRRLSSEPYGMLLVTGPTGSGKTTTLYAAISEVNHGYDKIIT
                     IEDPIEYQLPGVLQIPVNEKKGLTFVRGLRSILRHDPDKIMVGEIRDPETAQIAIQAA
                     LTGHLVFTTVHANNVFDVIGRFSHMGVDPYSFVSALNGIAAQRLVRLLCIHCAVEELP
                     EERLVAESGISPERARTFRFRTGKGCGQCRGSGYRGRNAIAEMLVLNDEIRELIVAKE
                     PIRRIKEAARRNGTRFLREAALEMVKAGQTSLQEANRVTIVA"
     gene            8761..9645
                     /locus_tag="VFI43_06825"
     CDS             8761..9645
                     /locus_tag="VFI43_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061875.1"
                     /translation="MSLSWRDRLQIFFAPGQVSITRFPKGLHYRAPKNGRLNYSWPKS
                     KALTTISAVCHEKPGKVLWASPLEELERCLDGVRGAEAVITISNHFVRYSVLPAQKDI
                     TNPAELNTYAEFHMREVFGERVAAWILSVGAWDPCYGGVCAAVDRELFEQLDELATRC
                     KVRLQYIEPYLTSAFDGFRKRFLDARSWFAVVEEGRLCLVLLEDGAWRHIRNRRIAHD
                     INDELLAALDQEAILFPGSKGVAGTVYLLAPAYPELILPEGCGWRIIWLQAGPMAAPS
                     NVRALPGPDEASTCAASG"
     gene            9627..10160
                     /locus_tag="VFI43_06830"
     CDS             9627..10160
                     /locus_tag="VFI43_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HET7061876.1"
                     /translation="MRSLRLKFPDDGGEARGGGYVLLVLGLAVLAGVLYQFNQAMNEV
                     AYWDLRIASMDRRVERKSAPAAAAARINETPETKREIRKANALLNELDLPWEALFDAI
                     EYASNHDVALLSFQPDPSSRKMRIGGEAKNIGAMLEFVGALEREPSLKDAHLLRYEIK
                     RDDPYRPVTFSLTATWG"
     gene            10157..10744
                     /locus_tag="VFI43_06835"
     CDS             10157..10744
                     /locus_tag="VFI43_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061877.1"
                     /translation="MNITPDVLSNTLLRIRWQAERLGNPGKIGLGLLVFSAVFFIAAV
                     LPRQEESSALRIKADEMQARLRSKPSEKEGDTAPKVVYGNQALKVFYAFFPNIDSTPF
                     WIGEVVKAAAQHGIELSGTDYRMEREKDIKLARYEMVLPVRGQYAQVRGFVADALRAV
                     PAMSLADVAIKREGVESDLLDANLKFNLYLSEGKN"
     gene            10777..11304
                     /locus_tag="VFI43_06840"
     CDS             10777..11304
                     /locus_tag="VFI43_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system X translation initiation
                     factor"
                     /protein_id="HET7061878.1"
                     /translation="MWLSGALVATLLAAQWVSGEEGGTDADQAAREEAPAAETRAPKA
                     RTASETPREVVEPEGLEIERLERRKFNAQAGDIFRQQSWAPPPPLPDPDNPPPPPPPP
                     PLQFKYLGKIIDGDEIRVFLALAERNYIVKPGENINSQYRMEEVNENSITFTYLPLNA
                     KQALSTIGGTAPESF"
     gene            11304..13571
                     /locus_tag="VFI43_06845"
     CDS             11304..13571
                     /locus_tag="VFI43_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HET7061879.1"
                     /translation="MRLQKYILIASVMLAFAGCATKNTALIEGNRLISEGNLDMGLAS
                     LEQAAREKPHDLQIRAVLARQRDAVTARLLFEADTARSSGDLDLAEQKYRHVLRLVPR
                     NERAQAGMETLDADRRHLALVKNAQELMERNEYTAAEPLIRQVLQENPLQPAARQLMQ
                     KITEMESELDRVGPVLKTAFTKPVTLEFRDAGLKSVFEIIARTAGLNFVFDKDVKQDT
                     KTTIFVKDARVEDIFKLLLLTNQLTHKVLNENSVLIYPNTPPKQKEYQDLMVRSFFVT
                     YTDVKQMVAMVKGLVKTKDVHIDEKLNLFVMKDTPEAIRLVARMVALNDVADPEVMLE
                     VEVLEIGRNRLLNLGLQYPDNIRVNMLAAGAALTGGAITPPGTFNISSEGVNGSGSNL
                     DRLTGFITNPAMIINLKQQDGIVNTLANPRIRVKNREKAKIHIGDKVPVVTTTATANV
                     GVASSVSYLDVGLKVDVESTISLKDDVSMKINLEVSTIVKEVQLPNSGIAYQVGTRNA
                     STTLALKDGETQVLAGLISDDERATLAKVPGLGDLPWVGKLFTNQNLTRNKTEIALLI
                     TPRIIRNIPRPSRASTELHFGTENNVGVVPVSIGKTPARSLGISPSSSGGSAGGMGIP
                     MPGNESPPGQLAASPRPDSPTVTIAAPEQALAGKEFGVSVNLSGAESLPSGEVELNYD
                     PTALELLDGGEKSGSRTVKVSKGGGSVELRFQVVAQKPGTTQVAVRNVAFRDESGNPI
                     PAQVVLPPVASIDIR"
     gene            13575..14084
                     /locus_tag="VFI43_06850"
     CDS             13575..14084
                     /locus_tag="VFI43_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET7061880.1"
                     /translation="MPKRWWGANRAKGAQEGFTLIEMTIVVAIMAILASSAVPLYELT
                     AKREKEQELRLALRQIREALDAYKRAVNEGRIAKQAEKKSDYPVKLEDLVDGVPDAKD
                     PGKRKIYFLRRLPRDPMSEDRSLSAAETWGKRSYESPPDNPQPGDDVFDIYSRSQVIG
                     LNGIPYDKW"
     gene            14071..14526
                     /locus_tag="VFI43_06855"
     CDS             14071..14526
                     /locus_tag="VFI43_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7061881.1"
                     /translation="MISGRNLRHRLPCGIGKRDIGRTVLLPAARSSGGFTLVELLVVM
                     AIIATLLSIVAPRYFNSLDRSRETVLRQDLNIMRDAIDKFNSDTGKYPADLAELVEKR
                     YLRAIPVDPLTESSETWVAVPPPNADSGIYDVRSGSSAEAKDGTSYADW"
     gene            14550..15269
                     /locus_tag="VFI43_06860"
     CDS             14550..15269
                     /locus_tag="VFI43_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET7061882.1"
                     /translation="MPNGREREKGFTYIWMMFTVAAIGLTLAAAGQSWRTQAQREKEK
                     ELIFAGDQLRRAIGSYYESSPGAKRFPPSLEKLLLDDRFPFVKRHLRKVFADPLTGKF
                     EWGLITQPGVGIMGVHSLSTGKPLKRANFHERYTDFEKAETYKDWKFIYSPGDPELAT
                     PQVQGRPVPEAPPPGGQRRGAGAQPPAPQGPSAGPAMQPGQFPGFPESNPTPDAVPDQ
                     DDSDPDSLEPGSVSSRMRDMP"
     gene            15504..16067
                     /locus_tag="VFI43_06865"
     CDS             15504..16067
                     /locus_tag="VFI43_06865"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET7061883.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEEVIVFRNDEISLDDIKRLKP
                     ARIVISPGPCTPSKAGISVPLIGHFHRDLPILGVCLGHQSIGQAFGGRIIHAGEIMHG
                     KTSPVFHNGRGLFRSLPDPFNATRYHSLVIERQSLPDCLEITAWTGDGEIMGIRHKTL
                     PIEGVQFHPESILTDHGHSLLANFLGR"
     gene            16111..17157
                     /gene="trpD"
                     /locus_tag="VFI43_06870"
     CDS             16111..17157
                     /gene="trpD"
                     /locus_tag="VFI43_06870"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7061884.1"
                     /translation="MKPQEALTRIVERDEILHDEMLSLMRQIMRGELSPALIAAIVFG
                     LRVKGETIGEIAAAAQVLREFALPLEVGDLDNLVDTCGTGGDSANTFNISTTAAFVAA
                     AAGARVAKHGGRSVSSRSGSADVLEALGINLDRTPREIAEDITEIGLGFMFAPNFHSA
                     MKHAAPVRRDLGIRTLFNILGPLTNPAGARNQLLGVFHPDLTGTLARVLQRLGSRHVM
                     VVHGGSGNVGLDEITVAGETCVSELRDGEVFEYRIKPEDFGLKSAPIEAIQVSEPLES
                     RQMMRAVLDNRPGPALDVVLLNAGAAIYVGGVAESLQEGVERARTTIESGAAEAKLRE
                     AVAFSNRRGTSANG"
     gene            17150..17983
                     /gene="trpC"
                     /locus_tag="VFI43_06875"
     CDS             17150..17983
                     /gene="trpC"
                     /locus_tag="VFI43_06875"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7061885.1"
                     /translation="MGDILQDILTVKFNEIAVAKAIKPLATVMREAEQAPQPRDFSGA
                     LRVKIAARRPAVIAEIKKASPSKGLLREHFDPGAIAASYALHGAACLSVLTDEQFFQG
                     SPDYLKQARNACSVPVLRKDFLIEEYQVLQSRAMGADCILLIVAAFGLLPPPSDAKLR
                     SEKIVGSMRKLERLSHSLGMAVLVEAHDAAELEIALELDSPLIGINNRNLRTFTTTLD
                     TTLTVLDQVPPDRIVITESGLRSPADIALMRNNNVHAFLIGEAFMRSDDPGVELARMF
                     G"
     gene            18265..19587
                     /locus_tag="VFI43_06880"
     CDS             18265..19587
                     /locus_tag="VFI43_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7061886.1"
                     /translation="MKKKLIALAVASALAAPVVASAQGTNVTIFGRAQAEYSLVDFST
                     QHSQGSINDNARSSRWGLHITEDLGNGFKANARLEFGLNTGAGSALTPREQWVGLSSN
                     QWGDVKFGRVQSPFKDFAGGMTIDPFAYTTLQANGAGGTMSGGANGLGSGANGFVNSA
                     IRLDSANIAGFSFAAIFMPGDANSLNPLNSGSIASSFLPGGLGSQGNTGGRNGDFDGQ
                     AAGKYHVDFMGMGLDVFGGYSRDNANHWQKAIGLKTEEVWRVGAAWTWENLKFAGQYE
                     QINNAIGSATCTTAASLTANPLDAASQGPTGLATGQCNSAMNWGGDGNIWHAMGSYRW
                     GNTTFVAQGGMTHANGTTGNGAIAGAAKRAADSFTVGVIHNLSKRTSVFGGYQHVYVR
                     NSNGSGANDLPASGDTASTNGAGGLSYAAPAYASGNRATYTIGIRHNF"
     gene            19920..21029
                     /gene="hpnH"
                     /locus_tag="VFI43_06885"
     CDS             19920..21029
                     /gene="hpnH"
                     /locus_tag="VFI43_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET7061887.1"
                     /translation="MGVPLRQQLAVAGYLLKQKLKGVKKYPMVLMLEPLFRCNLACAG
                     CGKIDYPDEILDKRLSYDECMQAVDECGAPMVSIPGGEPLIHKEMPEIVTGIVARKKY
                     VYLCTNALLLKKKIDDYTPSPYLTFSIHLDGLKERHDASVCQDGVFDRAVEAIKVAVA
                     KGFRVTVNCTLFQGETPEDVAEFLDYAMELGIEGATIAPGFSYERAPRQDIFIKGKDT
                     KNLFRDIFKIGKTRNWKLNHSTLYLDFLAGNQNYQCTPWGNPTRNIFGWQKPCYLLTD
                     EGYAASFQELLDDTPWEKYGNSSNPKCAQCMAHCGYEATAVEDALQHPLKALITSIRG
                     PKTTGPMVAEPTPKWATEQLKPRKTIGGIPVKIER"
     gene            21358..21990
                     /locus_tag="VFI43_06890"
     CDS             21358..21990
                     /locus_tag="VFI43_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpnM family protein"
                     /protein_id="HET7061888.1"
                     /translation="MNVVRTIKSFIFQIFAASLLIAPVYSSWAQSQSAGDPEQVVHTL
                     HEALIKAMREGEKLGYQGRFNLLAPVVDKTHDLDFIARTTLGANWTQLDAEQKRTFSE
                     IFRKLSIGTYAGWFKSYSGERFEFIESQPMPRDQVMVRSKLIQSDGEVVRFDYILRQG
                     KDGWRIINILADGVSDLALKRVEYRAILQRDGFSTLIDMLKKKLALTEQG"
     gene            22045..24735
                     /locus_tag="VFI43_06895"
     CDS             22045..24735
                     /locus_tag="VFI43_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7061889.1"
                     /translation="MTPSNKASDSRTYYLFARWATGCYSHARWVLILTLMLAAACGIY
                     IWRDLGMNTDTTDMLSKDLPFRVNLKHYNETFPQDMDTMLVVLEAPTPEQAQVSTKRL
                     TALLKRDAFNFFDVYSSDVDDFFSRNALLYESVPELARITDRLAAAQPLIAHIARNPS
                     LRAFADVISGAVNELRTGRDMELRSVLGGVSATLDARLSGSPRELSWQSLFQGEPQKQ
                     KYRDFIVVKPRLDYSQMFPAEQSISALRAAAKDIGITENSPVQLRITGEVALADDELN
                     ASLRGMEAAGVITFVLVGIVLYFAMRAGGLILNVLVCLTLGLILTAAFATAAVGHLNL
                     ISIAFAVLYIGLGADFAIHFLLRYRETLENGAGPQAAVHISGGEAGAALTACTITNAI
                     GFYAFMPTDYRGVAELGIISGTGMIISLLVTLTVGPALLRYLPGRLPAKSAKKKSPIG
                     RVLELSLKWHKATYAVTLAAAVGAVALLPQVRFDYNLLNLQDQKGEAIRTFRELLAET
                     DQSPWHAVALASSRSEADILAQRLAQLPEVSKVVNLTDFVPREQAEKLPLIEEMALTM
                     GPITISSGKRAADDAPADQLKSLEKLASTLDRFIADRPDHPAAQPARRLRSSLDKLLA
                     QLNSSSADEKQNLLSAVEDDLLAALPIALRNLQTATEAAPFNEQTLPESLRQRWRSQA
                     GEYRLAIYPAEDLNDNDALRRYVRAVQGVAPDATGAPMVSLEAGEAVVRAFAQAFMLA
                     LAGIVVALMIVLRSIKYTVLVLIPLLLSSLFTAAFTVLLNVPFNFANIIALPLLLGLG
                     IDSSLHMVHRSLNHELVSEVLIHTSTARAIFYSALTALVDFASLMLSSHRGTASMGIL
                     LTVGLAFTLICTLVILPVLLNAPTRTPIGVPRTAGRKDAG"
     gene            24979..25722
                     /locus_tag="VFI43_06900"
     CDS             24979..25722
                     /locus_tag="VFI43_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HET7061890.1"
                     /translation="MRPSEGSAETGKSEADEQIDPNEETNRKFYEFTDMIDRNVVAPV
                     ADVYIKYVPGPMQRSIGNFYDNLAYPNVILNDFLQGKVRQGFEDSLRFVVNSTAGAAG
                     LFDVATPLGLPQHDEDFGQTLGVWGVGTGSYLFVPLIGPSSYRDAPGLPVSLFTNVLF
                     YAGTFASGAFTAPITVLSIIDRRARLAGPMRVRDQAALDPYLFVREGFLQQRKHLIYD
                     GDPPPESFDEAIEEELSDEQANGKTRKAN"
     gene            25860..27818
                     /gene="shc"
                     /locus_tag="VFI43_06905"
     CDS             25860..27818
                     /gene="shc"
                     /locus_tag="VFI43_06905"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET7061891.1"
                     /translation="MKRSKTVARLVPNDRFTSSTNTKTNRTSPEDGLASARKALLSLQ
                     KDDGHWCFPLEADCTIPSEYVLMMHFMDDVDVALERRIAQFIREKQDAGHGGWSLYFG
                     GDFDLSCTIKSYYALKIVGDSPDAPHMARARAAILSHGGAARANVFTRLMLAMFGQIP
                     WRGVPFVPVEIMLLPKWFPFHLSKVSYWSRAVTVPLSILCSLKARAVNPRNVGIRELF
                     TVPPEEERDYFPTRTRLNRLLLMLERTGAYFEPFIPGPLRRLALRRAEQWTIERLNGE
                     CGLGAIFPAIVNAYEALALLGYPYEHPYREQCRRALLGLVIEEGERAWVQPCTSPVWD
                     TVLASLALQEDPEVDKKAVARALDWLIPNQVLDAPADWKDEHPGLAGGGWAFQYANPH
                     YPDLDDTAAAAWALYQGDPEAYRESLVRAADWLAGMQSRNGGFAAFDSDNTYYYLNEI
                     PFADHGALLDPPTSDVTARCAGFLALYGESRHKQNVERGLEFLFRDQEPSGAWFGRWG
                     SNYIYGTWSVLEGLRLIGLDTGHFAIRRAVQWLKSVQRDDGGWGESNYTYSAPEQAGR
                     FHCSTAFQTAWAVLALMAAGEGRSHEVRRGIEYLLYEQSEDGLWDDQWFTAPGFPKVF
                     YLKYHGYNKYFPVWALARFNAINRKISG"
     gene            27815..28519
                     /locus_tag="VFI43_06910"
     CDS             27815..28519
                     /locus_tag="VFI43_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HET7061892.1"
                     /translation="MREIGIVTAMRMEARCITRRRLPFNKRVVLGERTAIWLCGMGNE
                     AARKAAEGLKASGASALVSFGFAGALDSNLRPGDLVLPDTIHGRQSLPVDPGWRSRLR
                     KCLPASLNVLSGVLATSDIVLCSANAKREFGRATGACAVDMESGPVAEAAAVARIPFL
                     AVRVISDAVEFSPPPVLLNAIRPDGGADLSRLLPLLLRGAVTPSTLLRLAKESRVACA
                     TLSSVARHAEAELGIA"
     gene            28663..28932
                     /gene="rpsO"
                     /locus_tag="VFI43_06915"
     CDS             28663..28932
                     /gene="rpsO"
                     /locus_tag="VFI43_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7061893.1"
                     /translation="MPVTTDQKAQVVREYQRAAGDTGSPEVQVALLTARINDLANHFK
                     AHVKDHHSRRGLLRMVSRRRKLLDYLKQSSADRYRALIERLGLRK"
     gene            29068..31176
                     /gene="pnp"
                     /locus_tag="VFI43_06920"
     CDS             29068..31176
                     /gene="pnp"
                     /locus_tag="VFI43_06920"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7061894.1"
                     /translation="MNSIKKTISYGAHQLTFETGEIARQAHGAVMVSLDDTVVLVTVV
                     GAKTAKQGQDFFPLTVDYQERTYAAGKIPGSFFKREGRPSEKEILTSRLIDRPIRPLF
                     PENFYNEVQIVATVLSSDSEVDADIPAMLGASAALVLSGIPFAGPIGSARVAYINGEY
                     VLNPTTSELRQTQLNLVVAGTQRAVLMVESEASELSEDVMLGAITYGHQQMQSAIDAI
                     NSLADEAGVTAWDWVPPQRDTELATRIAELAEADLRAAFHMKQKQARSEAIDSIWTKV
                     FHELSVDAEGGPDAQAVKEACFALESRIVRSQILDGEPRIDGRDTRTVRPITIRHGLL
                     PRTHGSALFTRGETQALVVATLGTGRDEQKIDALQGDYSERFMLHYNMPAYATGETGR
                     VGTPKRREIGHGRLAKRALVAVLPTPEEFGYSMRVVSEITESNGSSSMASVCGGCLAL
                     MDAGVPLKAHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTEAGITALQMD
                     IKIQGITKEILHAALIQAKEGRMHILKIMKDALPVTRSDISEHAPRIIKIKINPEKIR
                     DVIGKGGAVIRALTEETGTTIDITDDGTVMIACVNAEGGELAKKRIEDITAEVEVGRV
                     YDGTVLKLLDFGAIVSVLPGKDGLLHISQIANERVNNVADHLKEGQVVRVKVLEADDK
                     GRLRLSMKAVNAETADDTAS"
     gene            complement(31352..31774)
                     /gene="dksA"
                     /locus_tag="VFI43_06925"
     CDS             complement(31352..31774)
                     /gene="dksA"
                     /locus_tag="VFI43_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HET7061895.1"
                     /translation="MPPELQKQVAPYKPKKGESYMSSKQLMHFRNILEGIKVELGHDI
                     DRTVHTMQDEATIFADPNDRASQESDMALELRNRDRERKLIKKIEETIAKIDTGDYGY
                     CESCGVEIGLKRLEARPTATLCIDCKTLDEIREKQVAK"
     gene            32117..32536
                     /locus_tag="VFI43_06930"
     CDS             32117..32536
                     /locus_tag="VFI43_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061896.1"
                     /translation="MRSKKSMLGIAVGSALTAGIHVAPASAADELFSVRELDKGYMVA
                     YADRVDPDKYGGGKPGGYGGGSGGGKTAEGQCGMSRIDVNKDGKVTKQEFLKHHETIF
                     DQIDANKDGAVDQPEADGYAAKVNAASNPAPHGAMKK"
     gene            32552..>33161
                     /locus_tag="VFI43_06935"
     CDS             32552..>33161
                     /locus_tag="VFI43_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET7061897.1"
                     /translation="MSGARLIEGTGLGLRRELIPALQSGVPAAIDFFEIAPENWIGLG
                     GKAERDLRALTEQFPFVCHGLSLSIGAPARLNEDLLRGIKKFLDLHNVSLYTEHLAYS
                     SDEGHLYDLLPIPFTGEAVKYVASRVRRAQDVLERRIALENASYYVQAPISEMSEADF
                     IRAVLVEADCDLHLDVNNVYVNSVNHAYDAEEFIHSMPRERIV"
BASE COUNT         7634 a         8607 c         9362 g         7558 t
ORIGIN      
        1 gaaaatatgt cgagttgctg catgagaata tggagtcaaa agcgaggaat caagaacgtt
       61 tgttcttatt atagagaaca attgttctta attcaattca gcaaaggaat atatcctgcc
      121 cttcctggga gagcagtcag ccctgctgcc acataaaaaa aacggccatc aacgatggcc
      181 gcctcaagat gaatctacgg aacttgatta atgacgggat gccgtttgtt gcgcgggtgt
      241 caactggtct ttccattcag gcggtagatg cctgacccag gtattgtaaa cattgggaat
      301 acccagaacc ccttgcccgc cgtaaccctc caggctggtg gtcgtatcat ccctggcggt
      361 gctgtggttg cttaccagtg aaatcctgcc cgcaaccacc gcgtctgata cgttcccatc
      421 attgtttgga tcgacatcca ccacgacaag ctcgttggag aacttgctgg tgacataggc
      481 atagtatccc ccgcctttct tggctccaaa attggcccca tggcagcctg gatcacaggg
      541 gagcatcttg gcgatcttgt ccgtagcggt atcgattacg gttatcgtcc cacccatatt
      601 cgcaaccact accgcttttc catccggaga aacgggtagc tggatgggaa gcattccaac
      661 cgttgcttgt ccatctccat cgcggtctgc tatcgtaccg tcaatggggt tgtaatcagc
      721 gatgagattg atggttttca acaaggtccc attcttgctg agcacgctca cattatggtg
      781 caacaggttg gctgcataga atttgcttcc atccggcatc atgctgatag cgatggggtg
      841 agccccgggc gcagactcac cgacgggcgt tctctccagg atgctgccat tcccgttgag
      901 attgtaaaaa ccaacgtcat tcgtaaagat gttgggcgta acgaccaagg cttcgccccc
      961 ctccacgctg atccagtgtc catgtggatt tgtggccggc tgaccggatt tctgcgtagg
     1021 catcatccgg acaacctccg tttttcctac tggaatcatg gatatcccgt cctcgccatt
     1081 gttcgtgaca gtaatgtcgt cagtgtcggg acgcgtcatg acgtgagccg gggcttcgcc
     1141 tacctggatg tttttgatca gcgctccggt tttctggttg atcatcgtca tcttgttatc
     1201 aaaccactgg gtctggaaga cgattgactg atccctgttg gtccacatgt tgtgtggatt
     1261 gttcatgttg atctggggaa gagcgatctt gcgcactgct ttccaggtac tggtgtcgat
     1321 cacagtaatc gttccgggct ttgtcttgcc tgccgttttc tcaaactgtg tatcgaccca
     1381 cacctctccc acaccttgga tagcgggagg gattggaggc gttagagcga tattgtttcc
     1441 atatctggca accagaacat ccgagaggtt gaccgtggct ccgcccgtaa tccggacggg
     1501 aacgttcggg taggttatat tccagtgtgc agaggaggca gggttctgcc aattattggg
     1561 agcagtggcg ataaagaaga tgctcagcag tcgggtggcc agatcgctgc tcgtggggag
     1621 attcacgatc ccggtagcca ggttgatggt ttccccaaga tcaagcccgt cggttgccgg
     1681 gtcatccacg atcaccgcgc caaacatgta aggatgtacc ttacaggtga aaacgtataa
     1741 accgggcgtc gtcagcttga caatcccgcc gcccttgtag gccctgcttt ggtcgaaagg
     1801 catggaggca tgttcgatat tcgctgcgcc ttgccctctt cttttgccct tgccttcatt
     1861 ggaatgatgt ccgttggaat gatgtccact ggaaggtaag cccgccgcac ttggccagag
     1921 caggctggtc atggagtgta cggtgtttgt ctcggccatg atgaaattca cgctgcttcc
     1981 gggcgaaatg acttcaaggg agcgcgttcc cttcgtgata ttgaaaatcg tttcgctgcg
     2041 cgcactccgg aaccagcttc ccggttcgtc cgaaattacg aaagtaacca ggccgggatc
     2101 cttgctagag ctgccgtaaa tggcgccgta gggagaagcc cctgcaatgc tgttgcccgg
     2161 ccccctgtgc gaaccgttgg cggcatcaac cctgaagctt ccgtgcatgc ccgcctccat
     2221 gtgggaaaag acgtggcaat gatactgcca gtgtcccggg cctacgcctg ttcccgcttt
     2281 cacggtaaaa gtatggctat cggaaccgtt ggcgagcagc ctgacatcga tggtgctttt
     2341 ggccccctgc tccagccagc gatgagcatg gaggtggaag gtgtggccag gtcctgctga
     2401 aagaacatgg aaccggacga tatccccctc gactgcgccg aaggtcgggt tggtccacag
     2461 cggcgtttgt gttccagcct ctgagatttc cgcaccccag aaggtcgagc ccaccatgaa
     2521 caaaacgtac tgcttggcaa gatctgctgc cttcactgac gtaatgacgc cgtcgccatt
     2581 gacgtagctt tccactttcc cgtccgttcc gtcgaccaca acggcgccga acagaccgag
     2641 cagttcctgg ccatcgccca gataggcgtg cgcgccggtt ttgctcgcgg tgaatgaaaa
     2701 gcttactgtt ccgcccggcg cggctttcgc ggcgctgccg tttttgagac tcggaaccct
     2761 gagcccgact ggtttaccag tgttgttggt caccgacaga gtgacagtat cgccttgctt
     2821 cacgaagaga gtcggtccag gaataacggc cttgtcttcc ccatttctct tgtatcccac
     2881 ctgaccgtta ggcagcactt cggcggacag cgagatgttg tgagccgccg cctgagctgg
     2941 aggtgacgaa acaagcgcag cggcaaagag cgtcaaggcc gctgatattg aagcgatcgc
     3001 tttctgtcca tgaaagatgt tgctcatatg ttcatccttt tgagtattgg gcagttagtg
     3061 gccgtggtga gctgcagtaa cgctgaaact gcctcccacg cccatcagtt tgcgtgaaaa
     3121 gttggcgttg acgtagcgcg cgctgttcaa cgccttgatc gtaaaaacgt gtttctcaag
     3181 tggcccgata tttttttctc gtatcaggtt gctcgtcccg ggatctatcc agtgataatc
     3241 cctgagctga aaacggttca agtaagtgcc catggcgatg atatggaacc ggacgtcgtc
     3301 gcccctgcca gcggtaaggt gagggttgtc tcccagggcc ttttgcttgt ggctatggcc
     3361 gtcaatttcc attccccaga atgagtcgtc gctgaggtaa agcacaaagt cttttttaat
     3421 gctgcgaacc ggcacgcttg tgatgttttc accatgtgcg gcaggcacgg agccggaggc
     3481 cgggttgacg atgattgcac cgtaaagccc cctgtcttcg gaaccgttgt gcgtttcata
     3541 gttgtggtcg tggtacggcc aggtgccggc tgtgccgggc gcgacatccc agaggtaggt
     3601 atagaagcgt ccgccgtgat ccggataggc accttcatct tcaattctgt tgataacctt
     3661 gagagagccg tcgctcaaga ttttgtaatg caccccatgc acatggacgc tgacctgctg
     3721 atccggggtc acggtaatgc cgttgcggat ggtcaggcga accttgtcac cctcggtgag
     3781 cacaattgtg ggccctggta tggtgggccc aggactgtac ctggaagtaa cattttcccc
     3841 atccaccaga tgctcaatca tctggtaggc aagcaatcca tccgccgttt gttcagcgat
     3901 catttcgatg cgatgatccg cagcaacggc gtgcgctgaa agaaaagatc ccaggagaag
     3961 agcgaatggg atgatcagct tggtatgaaa ggatttaagc tggggtgagc ccgcgaagaa
     4021 gggcggcttg ctggaagcaa aaaatttgct tacatcggaa tgatgttgac acttcatgcc
     4081 aggaaggctc ctttaaattg aaattgccca ggtttgagct aaaacggcag aacgccggtt
     4141 tatcaaggtt gaaacatgcc tccgactgcg ggaagaggat aagaatgttt ttcctcaaac
     4201 cgaatcacga ggtggcggga ttatgattga agagacttta aagaccgcgg gagggttgta
     4261 cctttttgat tactttgatg tactactctt ttttatcaat tttggtacat ctgaaccaat
     4321 acgggcagga acgcggaaga aagtcctgct aaattcctat aaaacaaaag cctgatatgc
     4381 ggggaagcca gggaaagccg gagcagagcc agcttggggg aaaacggaaa ttgagggcgt
     4441 ttagccgccg ttggatcggg caggcagcag atcagggtcg cttatgagat gctggtgaac
     4501 ctttgcaaac atgaatagat caaagcggtt tgtgatttcc agcttgctga aaatcgaggt
     4561 gagatggttg cgcaacgttt gttcgctgat acaaagcttc tgagctattt tcttgttcga
     4621 ggcacctgct tcgtttgcga agacgcagac gatggtgcgc tccttgcgag tgagggtcgt
     4681 aatttttttg gtgatcagat cagccggttt ctggccagcg cgtacgaaat caacaaacac
     4741 gcgtccaatt gtgcaacggt cgagccatag ttcgcctgca tgaacttttt cgatggcttt
     4801 gacgatcgtt tgagtcggtt cctccttgta aaccacgccg cgcgctccgc ttagcacagc
     4861 tttatcaacg tcagcctgat tgtgggcttc cgtataaatg ataatattga tttgcctttc
     4921 tcttaataag tcggaaataa catctacacc cctttccttt ccgagatgaa ggccgagcaa
     4981 aacgacatcg gggtccgttt ccctgctcaa ccgaagggcg tcgggacgat tggaggcctt
     5041 gccgacaatt tccatacggg gggactcggc attcaggagt ttctccagtc cccagagaac
     5101 gatttgatgg ctgttgacga gtagtatgcg gatggacatg acatcgtgcg agcagttcta
     5161 ctactctttt agatcagtat aagcgggttg caggttgtct gccagaaacg gataggtgcc
     5221 gacagtagac cgatacatgt gcattatagg cagatgctca ttttttaggc aattgtaaat
     5281 tttctatcct tttgcggatc gtccatggat acggcttgcg gcatttgtaa taagctaata
     5341 tgatttgtag ggtttttctg accaaatttt caggttaata ttcacgccaa gtcttatcgt
     5401 aaccttctgt catacggcga actttcgctc ggccccgcaa ggaatcctga aatcgagatg
     5461 aagtacgagc atatcaacaa ccgctccaaa gcggagtcag gatttacgtt gcttgaactc
     5521 ctcgtcgtga tggtaatcat tgggttgctg gccggttatg tcgcgccaaa atacttctcc
     5581 caggtcggca aatcagaagt caaggtagcc caggcgcagc ttgattcact gggaaaagcg
     5641 ctggatcaat acaggctgga tacggggcat tatccagcaa ccgaacaagg gctgggtgcg
     5701 ctgatgaccc ggccgacaaa cgaaccaagg tggcaagggc cctacctcaa aaaagcggcg
     5761 ccccctgatc catgggggcg tccgtacgcc tataagtatc cgggggaaca ttcggagtat
     5821 gacttgtatt cttacggaaa ggatggccag cccggcggaa acggcgaagc ggcggatatc
     5881 acaaactggt aggttgcgat gcgctacgag gtaaaagctg tgctggccag gcgaggcaca
     5941 gtgcttttac agctcgatgc tcaaagcgag gaggaggctc gtcttcaggt caccgcacag
     6001 ggtggaaggg tcttaaacgt ccggcggaga ttttccggct gggcatcaaa acagaaagtg
     6061 cgttttccgc tggcgcactt cagccatgaa ttgctatcgc ttctggtggc aggtctcagt
     6121 ctggttgaag cgatagaaac cttggctgac aaggagcagg acgtgggagt ccgccagatc
     6181 atccaggggt tgcttgcgcg cctctacgaa ggtgtcacgc tctccaaggc gctggagtca
     6241 ttcccgcagg catttcctcc gctttatgtg gcgacggcaa gggcaagcga gcgcactggg
     6301 gacttgcctg aagcattgac gcgctttata acttatcaga cccagatgga cctggttcgc
     6361 aaaaagctca tcggcgcatc catttacccc gttctcctcc tgaccatcgg cctgctggtt
     6421 gctttatttc ttctcgtttt cgtagtgccc aagttcagcg ccatctatga agatgctggg
     6481 ggagatttgc cgtggctttc catgctcctg atcagatggg ggcatcttgt ccaggagcat
     6541 gggtggcaac tggcggggat ggttggcgtt ttactcatca ttatcattta cggggtgcaa
     6601 cagcctgtgt tccggacgtg ggcttcggcg cagttgtgga aaattcccgc ggtaggggaa
     6661 cggatgaaag tttatcagct cgcgcgtttt taccggacgt tggggatgct gttgcgcggc
     6721 ggcataccgg tggtaaaggc cctcgagatg gtgagcggtt tgctgcaagc gcacttccgc
     6781 ccccgagttc acgccgccgc ggctcttatt cgcgagggca agaccatctc gagcgcgatg
     6841 caggccgagg gccttactac ccctgtagga agccgcatgc tccgtgtggg ggaaagaacc
     6901 ggacatatgg gtgaaatgat ggaacgcatc ggaagttttc atgatgagga aatagcccgc
     6961 tgggtcgagt gggcaaccaa attgatcgaa ccattgctga tggcgatcat cgggggcgta
     7021 atcgggggaa tcatcatcct gatgtacatg ccgatattcc agcttgcggg gagtgtgggg
     7081 tgagccagtt gcttgaatcg gagccgacac ctccagcaat gcccgaaatg gatgtggatc
     7141 agcttctgga gatccgcagg gcagcttctg cccgggaagt tccagttata agatacctgg
     7201 aggaagtatc cgggtggacg cctgaccgaa cactttcaga gcttggccgg ttactgcgga
     7261 tgcctgcgct gacgatggaa ggattgcggt cgctcgaaac agcatttgaa aaactgccat
     7321 ttaacgaggc aatcacacag gaatgcgcac tattccggag tggcgagcaa tatgtactgg
     7381 ctgtcggcaa tccttttctc ccgaatttaa gagcgtgggc ggatgaccgg attgatgttc
     7441 cagcagcctg gcatctcgtt catccatccg atctggctgc ttttttcacg aagcaagagc
     7501 agtctatgcg ggccatggat agcgtgcttc cgggagcgca acagcgttcc caacaggtgg
     7561 gcgaagaaaa cctctcgatc aagtccatta acgaagggac gagccaggtc gtgaggttgg
     7621 ttcactccac gctctatgac gcgcataaat cacaggcaag tgatattcat ctggagatgg
     7681 tgacaggtgc tctatcgatc aaatacagga tcgacggcgt gctgagcagt atcggggtgg
     7741 tgcagggtgc cgaccttgcc gaacaggtca tttcacggat caaggtaatg tctgacctgg
     7801 atattgccga gcgtcgcgtg ccgcaggatg ggcgcttcaa aatttccatt caaggccggg
     7861 agatcgattt ccgcgtctcg atcatgccca gcagtttggg cgaggatgca gtgttgcgta
     7921 ttcttgaccg gcaggcgctg gcagaccatg tcgaaggctt gaagctcgac catctggggt
     7981 tcgacgcgcc taccatgtcc agcctgaggc gcttgagttc cgaaccatac ggcatgctgc
     8041 tggtgaccgg accaacgggg agcggcaaga caacgacgct ttacgcagca atttccgagg
     8101 tcaaccacgg ctatgacaag attatcacga ttgaagaccc aatcgagtat cagttgcccg
     8161 gcgtgctgca gatacccgtc aacgaaaaaa aaggactcac gtttgtccgc ggtttgcgct
     8221 ctatcctgcg tcatgatccg gacaagatca tggttggcga aatccgtgat cctgaaacgg
     8281 cgcaaatcgc catacaagca gctctgaccg gacacctggt attcacgaca gtgcatgcga
     8341 acaacgtctt cgatgtgatc gggcgttttt cgcatatggg agtcgatccc tacagtttcg
     8401 tgtccgcttt gaatggaatc gctgcacaac ggctggttcg cctgctttgc atccattgcg
     8461 cggtggaaga actgccggaa gagcgtcttg ttgcagagtc ggggatcagt ccggagcgtg
     8521 cacgtacgtt caggttccga acgggcaaag gctgcgggca gtgccgggga agcggttacc
     8581 gggggagaaa tgcaatagct gagatgctgg ttcttaatga tgaaatcagg gaacttatcg
     8641 tcgcaaagga acccatacgc cgaatcaagg aagcggcgag acgaaacgga acacgctttc
     8701 tacgggaagc ggcgcttgag atggtaaagg cagggcaaac aagtctacag gaggccaatc
     8761 gtgtcactat cgtggcgtga ccggttacag atttttttcg cccctgggca agtgtccatt
     8821 acgcgtttcc ctaaagggtt gcattacagg gcgccgaaaa acggcaggct gaactatagc
     8881 tggccgaaat caaaggcttt gactaccatt tcagccgtat gccacgagaa gccggggaag
     8941 gtgctttggg cctctcctct tgaagagctc gagcgatgtc tcgacggagt tcggggcgca
     9001 gaggccgtta tcacgatttc aaaccacttt gtccgatatt cggttctgcc tgctcagaaa
     9061 gatattacga atcctgccga actgaatact tatgctgaat tccatatgcg ggaggtgttt
     9121 ggagaacgag tggccgcctg gattctcagt gtaggcgcct gggatccctg ctacggagga
     9181 gtatgcgcgg ctgttgatcg cgaactgttc gagcaacttg atgaacttgc cacccgctgc
     9241 aaagtgaggc tgcaatacat agagccgtac ctgacaagcg cgttcgatgg tttccggaag
     9301 cgcttcctag atgcacggtc atggtttgca gtggtggaag agggacgcct atgcctggtg
     9361 ttgcttgagg acggagcctg gcggcacatc agaaatcgca ggatagcgca tgatatcaac
     9421 gacgagttgc tggcagctct cgaccaggaa gctatacttt ttccaggaag caagggagtt
     9481 gccggaacgg tttacctgct ggcacccgcg tacccggaat tgatcctgcc ggagggttgc
     9541 ggttggcgaa tcatttggct gcaggctggt cctatggccg cgccctcgaa cgtgcgagct
     9601 cttcccggtc ctgatgaggc atcgacatgc gcagcctcag gctgaaattc ccggatgatg
     9661 gaggcgaagc ccgtggaggc ggctatgtat tactcgtgct tggcttggca gtgcttgccg
     9721 gagtgctgta tcagttcaac caggccatga acgaagtagc gtactgggat ttgcggattg
     9781 ccagcatgga ccggcgagta gaacgcaaat ctgctcctgc cgctgccgct gcccgtatca
     9841 atgagacgcc tgaaacgaag cgggaaatca gaaaagcaaa cgcgcttttg aatgagcttg
     9901 acctcccttg ggaggcttta tttgacgcca ttgaatacgc ctcaaaccat gatgtggcgc
     9961 tgctgtcgtt tcagcccgat ccttccagcc gcaaaatgcg tatcggcgga gaagcaaaaa
    10021 acattggcgc aatgctcgaa ttcgtcggtg cactggaacg ggaaccctct ctcaaggatg
    10081 ctcacctcct gaggtacgaa atcaagcggg atgatcccta tcgtccggtt actttctccc
    10141 ttaccgcaac atggggatga acataacgcc cgatgtttta tctaatacgc tgctacggat
    10201 acgctggcag gcggagcggc tggggaatcc cggaaaaatc ggcctgggcc tgcttgtctt
    10261 ttcggcggtt tttttcattg ctgcggtgct gcccaggcag gaggaatcca gcgcgctgag
    10321 aatcaaggcg gatgaaatgc aggcgcggtt gagatcaaaa ccctcagaga aagagggcga
    10381 taccgcccca aaggttgttt atggcaatca agcattgaaa gttttttacg cttttttccc
    10441 aaatattgac tcaacgcctt tctggattgg agaagtggtc aaggccgcag cgcaacatgg
    10501 tatcgagctg agtggtaccg actatcgtat ggagcgggaa aaggacataa agcttgcgcg
    10561 ttacgaaatg gtattaccgg ttcgggggca atatgcacag gttcgcggct tcgtcgccga
    10621 tgcattacgg gcggttccgg ccatgtccct tgcggacgtt gccataaagc gcgagggcgt
    10681 cgaatccgac ctgctggatg cgaacctgaa attcaatctc tacctgagtg aaggcaaaaa
    10741 ttgaaacggg gccagccaca aggacatgga cgcgtaatgt ggctgagcgg cgcgctagtc
    10801 gcaacgctcc ttgctgccca atgggtgagc ggcgaagaag gaggaacgga tgcggatcag
    10861 gctgcgcgag aagaagctcc ggcagccgaa acacgcgcac caaaagccag aacggcgagt
    10921 gagacgcccc gggaagtagt ggaacctgaa gggctggaga tcgagcgtct cgagcgcagg
    10981 aaattcaacg cgcaagcagg ggatatcttc cggcagcaat cctgggcacc gccgccgcct
    11041 ctacctgacc ccgataatcc accgcctcca ccaccgccgc ctccgttgca gttcaagtac
    11101 cttggcaaaa tcatagatgg ggatgaaatt cgtgtatttc tcgcccttgc cgagcgtaat
    11161 tacatcgtga agccgggaga gaatatcaat agccaataca ggatggaaga agtgaacgag
    11221 aactccatta cgttcactta ccttccgctt aacgcaaagc aggcgctttc cacaatcggc
    11281 gggactgcgc cagaaagctt ttgatgagac ttcaaaagta tatcctcatc gcctcggtaa
    11341 tgctggcgtt cgccggatgc gcgacgaaga acacggcgct gatcgagggg aaccggctga
    11401 tttctgaagg caacctggat atgggactcg cgagcctgga acaggcagcg cgcgaaaagc
    11461 cgcatgacct tcaaatccgt gccgtactcg cccgccagcg tgatgcggta accgctcgtt
    11521 tgctgtttga agcagatact gcgcgatcat cgggtgatct tgacctggcc gagcaaaaat
    11581 accggcatgt gcttcggttg gtccccagga acgaacgcgc tcaggcggga atggagactc
    11641 tggatgccga ccgccgtcac ctggcgctgg taaaaaacgc gcaggagctg atggaacgca
    11701 atgaatatac ggcggcggaa ccgctgattc gccaagttct ccaggagaat ccattgcagc
    11761 ctgctgcccg ccagctcatg cagaagatca ccgagatgga atcggaactg gacagggtcg
    11821 ggcctgtact gaaaaccgcc tttacaaaac cggtaaccct cgagtttcgc gacgccggcc
    11881 tgaaatcagt ttttgaaatc attgcccgca cggctggatt gaattttgta ttcgacaagg
    11941 acgtcaagca ggacaccaaa acgaccattt ttgtgaagga tgcaagggtg gaggatatat
    12001 tcaagcttct gctcctgacc aatcaactca cccacaaagt ccttaacgag aattcagtcc
    12061 tgatctatcc caacacgccg cccaagcaga aggaatacca ggatttgatg gtacgcagct
    12121 tctttgtgac gtataccgac gtaaagcaga tggtcgccat ggtcaagggc ctggtcaaga
    12181 ccaaggacgt gcatatcgac gagaagctga atctttttgt gatgaaggac actcccgagg
    12241 caatacgcct ggttgcgcgc atggtcgccc tgaacgacgt tgcggatcct gaagtcatgc
    12301 ttgaggtgga ggtcctggaa atcggccgta atcgcctgct taatctcggc ctgcagtatc
    12361 cggacaatat cagggtaaat atgcttgcgg ctggagctgc ccttacgggc ggcgccatca
    12421 cacctcctgg gactttcaat attagcagtg aaggagtcaa tggaagcggg tcgaaccttg
    12481 accggctcac cgggttcatc accaaccctg ccatgatcat caatctcaag caacaggatg
    12541 gcatcgtcaa cacccttgcg aatcctcgaa ttcgggtgaa gaaccgtgag aaagcgaaaa
    12601 tacacatcgg tgacaaggtg cctgtcgtaa ctaccactgc cacggccaac gtcggcgttg
    12661 catcatcggt cagctacctc gatgtagggc tcaaggtaga tgtcgaatcc acgatttcgc
    12721 tgaaggacga cgtctcgatg aaaatcaacc tggaagtcag cactatcgtg aaagaggtgc
    12781 aactgcccaa cagtggaatc gcttaccagg tcggtacccg aaacgcatcg actacgcttg
    12841 cgttgaagga tggggaaacg caggtattgg caggactgat cagcgacgat gagcgcgcaa
    12901 cgcttgccaa agtccccggg cttggagatc tgccctgggt gggcaaactg ttcacgaacc
    12961 agaatttgac ccggaacaag actgaaattg cattgctgat aaccccgcgc attatccgga
    13021 acatcccccg tccgtcgagg gcatccaccg agcttcattt tggaacggaa aataatgtgg
    13081 gggtggttcc ggtcagtatt ggcaaaacgc ctgcccgttc gctcggaatt tcgccgtctt
    13141 cttcaggtgg aagtgccggg ggcatgggga ttcccatgcc cgggaacgaa tctcccccgg
    13201 gacaattggc tgcttctcca aggccggact ctcctacggt aaccatagca gccccggaac
    13261 aggcgctggc agggaaagag tttggggtca gcgtcaatct atcgggtgct gagtcattgc
    13321 cttcaggcga agtggagctg aattacgatc ctactgccct tgagttgctc gatggcggcg
    13381 agaagtcggg tagccggaca gtgaaggtga gcaagggtgg cggtagcgtg gaattgcggt
    13441 tccaggtggt tgcccagaag cctggcacca ctcaggttgc agtcaggaac gtcgcattcc
    13501 gggatgaatc cgggaatccc attcccgcgc aggttgtgtt accgcctgtc gccagcatcg
    13561 atatccgttg atccatgcca aaaagatggt ggggagcaaa ccgggcgaaa ggcgcacagg
    13621 aaggatttac cctgatcgaa atgaccatcg tggtggcgat catggctatt ctggcttctt
    13681 cggctgtgcc actgtatgag ttgacagcca agcgtgaaaa agaacaggaa ctgcggctcg
    13741 cgctaaggca aatccgggaa gcgcttgatg cgtacaagcg tgcggtcaac gaaggcagga
    13801 tcgccaagca ggcggaaaaa aaatcggatt atcccgtgaa attggaagac ctggtggatg
    13861 gggttcccga tgcgaaggat ccgggaaaac gcaagatcta cttcctgcgc cggctgcctc
    13921 gagacccgat gtcggaggat cgttcgttat ctgctgctga aacctgggga aaacgctcgt
    13981 acgaaagccc ccccgacaat ccacaaccag gcgatgacgt ttttgatatt tattcacgct
    14041 cgcaggtgat cggcctgaac ggcattcctt atgataagtg gtaggaactt gcgtcaccgg
    14101 ctcccttgcg ggataggcaa gcgggatatc ggaagaaccg ttctgctgcc tgcagcgcga
    14161 tccagcgggg gcttcacgct ggttgaatta ttggttgtca tggctattat cgcgactctc
    14221 ttaagcattg tcgccccccg atatttcaat tcgctggatc gctccaggga aactgtgctg
    14281 aggcaggatc tcaacatcat gcgggacgcg atcgacaagt tcaatagcga taccggcaaa
    14341 tatcccgccg acctggctga acttgtggaa aaacgatacc ttcgggcgat tcccgtggat
    14401 cctttgaccg agagttccga aacgtgggtt gcagttccgc ctcccaatgc tgattcaggt
    14461 atttatgacg tgcgtagcgg ctcgtctgcc gaggcgaagg acggcacctc atatgcggac
    14521 tggtagaagt ggcaagcgtg gaaggagtca tgccgaacgg cagggaaagg gaaaaggggt
    14581 tcacctatat ctggatgatg tttacagtgg cagcgatagg cttgacactg gctgctgcag
    14641 gacagtcatg gcgcacacag gctcaaaggg agaaagaaaa ggaacttatt tttgcgggag
    14701 atcagttaag aagggcgatc ggctcgtact acgaaagctc acccggggcc aagcgttttc
    14761 caccttccct ggaaaaactg cttctggacg accgctttcc gttcgtcaaa cggcatttgc
    14821 gcaaggtctt tgctgaccct ctgacaggaa aatttgagtg gggacttatc acgcaaccgg
    14881 gggtcggaat catgggcgtg cacagccttt caaccggaaa gccgctcaag cgggcaaatt
    14941 ttcacgaacg ctacactgac ttcgaaaaag cggaaaccta caaagactgg aaatttatct
    15001 attctcccgg agatcctgag cttgctacac cgcaggtaca gggccggccg gtgccggaag
    15061 caccgcctcc ggggggccag cggcgaggcg cgggagccca gcctcccgca ccccaggggc
    15121 cgtcagctgg gcccgcgatg cagcctggac aatttcctgg atttcccgag tcaaatccaa
    15181 cccctgacgc agttcccgac caagacgatt ccgatcctga ttcacttgag ccggggtctg
    15241 tctcatcgcg gatgcgggac atgccgtagt tgttttttag cgggccgtag agctgttagt
    15301 acagccaccc ggcgggaagc cgtatcttgc ctttagctca tgcgctgtag gtaaaagcag
    15361 tttccccgta atccattcgc catcccttat ttcttgccca tgcctgcgtg agcaagggat
    15421 aatagaggtt atttgctgcg aaacccttca agcatgggtt aacctcgtgt tcatgatttc
    15481 gcctatcggc cacgaactca agtatgctgt tgatgatcga caactacgat tccttcacct
    15541 ataacctcgt acagtatttt ggcgaactgg gtgaagaagt aatcgtattt cgtaatgatg
    15601 agatcagtct cgacgatatc aagcggctga agcccgcgcg cattgtcatt tctcctggcc
    15661 catgcacgcc gagtaaagcg ggaatttccg ttccattaat cggacatttt caccgcgacc
    15721 ttcccatatt aggcgtatgc ctgggacatc aaagcatagg gcaggccttc ggggggagga
    15781 tcattcatgc tggagaaatc atgcacggga agacctcccc cgtgttccat aatggccggg
    15841 gcttgttccg cagcttgccg gatccgttca acgctacccg ctatcactcg ctcgtgatcg
    15901 aacgtcaaag cctccccgat tgcctggaga ttacggcatg gacaggggat ggggaaatca
    15961 tgggaatacg ccacaaaacc ctgcccatcg aaggcgtcca gttccaccct gaatccatcc
    16021 ttaccgacca tggccacagc ctgctcgcca acttcctcgg aaggtgagaa aaaagatgca
    16081 ccagaagcag agcagtacat cgaaaacgtc atgaagccgc aggaagcgct gacccgtatt
    16141 gtcgagcggg acgaaatttt gcacgacgaa atgctctcgc tcatgcgcca gatcatgcga
    16201 ggcgaactgt cgcctgcgtt gatcgcagca atcgtgttcg gtcttagggt aaaaggggag
    16261 accatcggtg aaatcgcagc ggcggcacag gtacttcggg aatttgcctt gccccttgag
    16321 gtgggggatc tggataatct ggtcgacacc tgcggtacag gaggcgattc agccaataca
    16381 tttaatattt ccacgaccgc ggcttttgtt gcggcagcag ctggcgctcg agttgcgaag
    16441 cacggggggc gatccgtttc gagcaggtcg gggagcgctg atgtgctcga ggcgcttggc
    16501 atcaatctcg atcggacgcc gcgcgagata gcggaggata ttacggaaat aggcctgggc
    16561 ttcatgttcg cacctaactt tcacagcgcc atgaaacatg cggctcccgt ccgacgagac
    16621 ctcggcattc gaactctatt taatattctt ggcccgctca ccaatccagc gggagcaagg
    16681 aatcaactat tgggcgtgtt tcaccctgat ctcaccggca cgctcgcgcg ggtgcttcag
    16741 cgattgggca gccgccatgt catggtggtc catggcggaa gtggtaacgt ggggctcgat
    16801 gagattaccg tagctggcga aacgtgtgtc agcgaactca gggatggaga ggtttttgaa
    16861 tacaggataa agccggaaga tttcggattg aaatcagctc caattgaggc aattcaagtc
    16921 agcgagcctc ttgagtccag gcagatgatg agggcggtgc ttgataatcg cccaggccct
    16981 gcgctggatg tcgtcttgct gaatgcgggg gcagccatct atgtgggggg cgtggcagag
    17041 tccctgcaag aaggtgtgga acgggctcga acgacgatag aaagcggggc agctgaagcg
    17101 aaattgaggg aagccgtcgc attctcgaac aggcgtggga cttcagccaa tgggtgatat
    17161 tctgcaggac atcctgacgg taaagtttaa tgagatcgct gtggcaaagg cgataaaacc
    17221 attggctacg gtgatgcgcg aggcggagca ggcgccacag ccgcgggatt tttccggtgc
    17281 gctgcgcgtt aaaatcgccg ccaggcggcc cgcggtcatt gccgagataa aaaaagcgag
    17341 ccctagcaaa gggctgttgc gggagcattt tgatcctggc gctatcgccg cgagttatgc
    17401 gcttcatggc gcagcctgtc tatccgtgct tacggacgag caattttttc aaggcagtcc
    17461 cgactatctg aagcaggcaa gaaatgcatg cagcgtcccg gtccttcgaa aggacttttt
    17521 aatagaggaa tatcaggtat tgcagtctcg agcaatgggg gcagactgca tcctgctcat
    17581 cgtggccgcc tttggcctgc tccccccccc ctcagacgct aaactgcgct cggagaagat
    17641 cgtgggcagc atgcgaaagc tggagaggct ttctcattcg ctgggaatgg cggtgctggt
    17701 cgaagcgcac gacgctgcag agctcgagat tgcgctggag ctcgattccc ctctgatagg
    17761 aatcaacaat cgtaatttgc gcactttcac gaccactctt gatactacct taaccgtgct
    17821 cgatcaggtt ccacccgatc gtatcgtgat cacggagagc ggcctccgga gcccagcaga
    17881 tatcgcgcta atgcgcaaca acaatgtcca cgcctttttg attggagagg ctttcatgcg
    17941 atcagacgac cctggggtcg aactcgcccg gatgtttgga taagacctcg aaaatatgga
    18001 taaatttgcg gcgtcaagga tcgatcggca gggaaaaaac cgcgaaggtt cgatttggta
    18061 acagttcaag gcgactttgt tgcaaaaaag caacaaacgg tccgacgatc tgaggaaagt
    18121 ctctttattt ccttgctagg tgttgccgcg ttaggcacaa tctctcaacc ctctcatcgt
    18181 gagaggtcca ggcagcggga tttactccac cgaatagggt cggtgcgagg ccccaccgtt
    18241 tcaactttaa aggagatttt ccacatgaaa aagaaactga ttgcactggc ggttgccagt
    18301 gcactcgcgg ccccggtggt tgcatccgct caggggacca acgtcactat ctttgggcgg
    18361 gcacaggccg aatacagcct ggtcgatttt tctactcaac atagccaagg aagcatcaac
    18421 gacaacgccc gttcttcgcg ttggggtcta catattaccg aggatctggg caacgggttc
    18481 aaggccaacg cccgcctgga attcggcctc aataccggcg ccggttccgc gcttacgccg
    18541 cgggaacaat gggtcgggct ttccagcaac cagtggggtg atgtcaagtt tggccgcgtg
    18601 cagtccccct tcaaggactt tgccggaggc atgacgatcg acccatttgc atatacgact
    18661 ctccaagcta acggtgcggg cggtaccatg tctggtggcg ccaatggtct cggctcgggc
    18721 gcgaacggtt tcgtgaacag tgcgatccgt cttgattcgg ccaacatcgc gggcttctcc
    18781 ttcgccgcca tattcatgcc tggcgatgcc aattccctca atccgctcaa ctccggcagc
    18841 atcgcatcga gcttccttcc cggtggtctc ggcagccaag gcaatacggg tggccgcaat
    18901 ggtgatttcg atggccaggc cgccggtaag taccatgtcg atttcatggg catggggctt
    18961 gacgtattcg gcggctactc gcgcgacaat gccaaccact ggcagaaagc gattggcctg
    19021 aagactgaag aagtatggcg ggttggagct gcctggacct gggaaaacct gaagtttgcg
    19081 ggccaatatg agcagatcaa taacgcaatt ggttctgcaa cctgtaccac cgccgcttcg
    19141 ctgaccgcaa atccgcttga cgccgcgtcg caaggcccaa cgggactggc taccggacaa
    19201 tgtaactctg ccatgaactg gggcggagac ggaaacatct ggcacgcgat gggtagctac
    19261 agatggggta atactacttt cgtggctcaa ggcggtatga cgcacgctaa cggtacaacg
    19321 ggcaatggcg ctattgcggg cgccgccaaa cgtgcagcag acagcttcac ggtcggcgtc
    19381 attcacaacc tgagcaaacg caccagcgtt tttggtggct atcagcacgt ctatgtgagg
    19441 aatagcaacg gttcaggggc aaacgacttg cccgcctccg gcgatacggc cagcaccaac
    19501 ggggcaggcg ggctcagcta tgctgcgccg gcgtatgcaa gcggaaatcg tgcgacctac
    19561 acgatcggta tccgtcacaa cttctagtag aaacaaggaa atttaaaatg gggcgcctgg
    19621 cgccccattt ttttgctcta ccatgacatt gaagcaagca gggtgatatc aaactgcaca
    19681 ggccgaagcg agccgaatat cgcaatctcc cttgagaact tacttgggcg ctttatcggt
    19741 tccggaggct gctgccggac gtagctcttt gattcaatgt gcttaacctt gaaatgtttg
    19801 tgtgtttagt ttgtaaaaca tccgtggtac catacaagcc cattcagcaa cacaacaata
    19861 tggagcggtt ttagaccgca cccctcaaca gtttatttga agttttttgg agagtcgtca
    19921 tgggagttcc tttacgtcaa cagttggctg tagccggtta tctgctcaag caaaaattga
    19981 aaggcgtcaa aaaatatcct atggttctga tgctggaacc cctgttccgc tgtaacctgg
    20041 cttgtgcggg atgcggcaag attgattatc ccgacgagat tctggacaaa cgcctttcgt
    20101 atgatgagtg catgcaagcg gtggacgaat gcggcgcccc aatggtatcg attcctggag
    20161 gcgagcctct catacacaag gaaatgccgg agatcgttac tggcattgtc gcacgaaaaa
    20221 aatatgtcta tctgtgcact aacgcactct tgttaaagaa gaagatcgat gactatacgc
    20281 cgtctcccta tctgactttc tcgatccacc tggatggatt gaaggaacgg cacgacgctt
    20341 ccgtgtgtca ggatggcgtt ttcgaccggg cggttgaagc catcaaggtc gcggtagcga
    20401 aaggattccg cgttaccgtc aactgcactt tatttcaggg tgaaacgccg gaagatgtgg
    20461 ccgagtttct ggattatgca atggaactgg gaatcgaagg cgcgaccata gcaccagggt
    20521 tcagctatga gcgggcgcca agacaggata tcttcatcaa gggtaaggat accaagaatc
    20581 ttttcagaga tatcttcaag atcggaaaga cccgcaactg gaagttgaat cactcgacac
    20641 tctatctcga ttttcttgcc ggcaaccaga attatcaatg caccccttgg ggaaacccta
    20701 cccgcaacat cttcggttgg caaaaaccct gttatttatt aaccgatgaa ggttatgcgg
    20761 caagttttca ggaattgctg gatgacaccc cctgggaaaa gtatggcaac agcagcaacc
    20821 cgaaatgcgc gcagtgcatg gcacattgcg gctatgaagc gactgctgtt gaagacgccc
    20881 tgcagcaccc cttgaaagct ctgatcacct cgattagagg gccgaaaacg acaggtccca
    20941 tggtggccga accgacccca aaatgggcaa ccgagcagct taagccgcga aagacgatcg
    21001 gtggaattcc ggtaaagata gaacgataag ctatactcaa tctaagcatt ttggctcagg
    21061 aatgagctaa atgacgagtc ccggtcgaaa tgcttgaagt gagcctgata taagcgcccc
    21121 ggacagttcg ttcggggcca cttcgttcat aggcattttg acgaaatgct cgatttggga
    21181 atggatttgt tttgctagag caattaatcc gcaatggcta ttcggatcgg gaggcattga
    21241 gacctgttgc cccgggagcg cttcccggga ggcggctagc tagtttcgca tgaattcgta
    21301 tcgcagtagt cacccaggcc ctgggtgcta aaaccaaggc gtaaacaggt tctcaatatg
    21361 aatgtcgtgc gtacaatcaa gtctttcatc ttccagatct tcgcagccag cctgttgatt
    21421 gcgccggttt attcctcttg ggctcaatct cagtcggcag gggatccgga gcaggtggtg
    21481 catactctcc acgaggcact tatcaaagca atgcgggagg gagaaaaact aggctaccaa
    21541 gggcgattta atctgctcgc tccagtcgtg gataagactc atgatctcga cttcatcgcc
    21601 cgcaccactc tgggggcgaa ctggacgcaa ctggatgcag agcagaagcg cactttctct
    21661 gagatcttcc gcaaactgag tattggcact tacgcgggct ggttcaaaag ctatagcggc
    21721 gaacgtttcg aatttattga gagccagcct atgccgcggg atcaggtcat ggtgcgcagc
    21781 aagctcatcc agtctgatgg tgaagtcgtg cgattcgatt atattttgcg gcagggcaag
    21841 gatggctggc gcatcatcaa cattctggcg gacggtgtca gcgatcttgc actcaaacgc
    21901 gttgaatacc gcgcaatcct tcaacgcgac gggttttcca ccctcatcga tatgctcaag
    21961 aaaaagcttg ccctcaccga gcaaggctga aaaaccgccc cgctgtatgg gcagttgatc
    22021 gcccaagccg ttctatcccc aaggatgacc ccttccaaca aggcttccga cagccgtacc
    22081 tactacctct tcgcgcgctg ggcgaccggg tgctatagcc acgcccgctg ggtgctcatc
    22141 cttaccttga tgctcgccgc tgcatgcggc atctatatct ggcgggatct tggcatgaat
    22201 accgacacca cggacatgct ctcaaaggac ctcccgtttc gcgtcaacct gaagcactat
    22261 aacgaaacgt ttccgcagga catggatacg atgctcgtcg tcctggaggc gcccacaccc
    22321 gagcaggccc aagtatccac caagcgtctc acagcgctgc tcaagagaga tgcgttcaat
    22381 ttctttgatg tttattcatc cgacgtcgac gatttttttt cccggaacgc actactatac
    22441 gaaagcgtcc cagaactcgc gcgtatcacc gatcgcctgg cggctgccca gccgctcatt
    22501 gcccatattg cgcgcaatcc gagccttcgg gctttcgccg atgtaatcag cggagcagtg
    22561 aacgagttgc ggaccgggcg agacatggaa ctacggtcgg tattaggcgg agtgtcggca
    22621 acccttgatg cgcgcctctc cggctcacct cgagagctct cgtggcaatc cctctttcag
    22681 ggtgagccgc aaaaacaaaa ataccgggac ttcatcgtag tcaagccgag gctggattat
    22741 tcgcaaatgt ttcccgcaga acagtccatt tcggcacttc gcgccgcagc aaaggatatt
    22801 ggcattaccg aaaacagccc tgtccagtta cgcataaccg gagaggttgc tttagccgat
    22861 gacgagctga atgcttcgtt acgcggcatg gaagcagcag gagtcatcac ctttgtcctc
    22921 gtcgggatcg tgctttattt cgccatgcgc gccggagggc tgatcctgaa cgtcctggta
    22981 tgcctgacgt tagggctgat cctcacggcc gcgtttgcga cggcggcggt tggccatttg
    23041 aaccttatct ccatcgcatt cgcggtttta tatatcggcc tgggggctga tttcgccatt
    23101 catttcctgc tgcgctaccg ggaaaccctg gaaaatggag cgggccctca ggcagccgtg
    23161 cacatatcag ggggagaggc aggtgctgcg ctcaccgctt gcacgatcac caacgccatt
    23221 gggttttatg cattcatgcc cacagattac cgaggcgtag ctgaattggg aatcatctct
    23281 gggaccggca tgattatcag cctgctggtc acccttacgg taggccccgc attgcttcgc
    23341 tatcttccag gaaggctgcc tgcaaaatct gcgaaaaaga agagtccgat cggacgggtg
    23401 cttgaactgt cactaaagtg gcacaaggca acatatgcgg ttacccttgc ggccgccgtc
    23461 ggcgccgtcg ccctcctgcc gcaagtcagg tttgattaca accttttgaa cctccaggac
    23521 caaaaggggg aggcgataag aacctttcga gaacttctcg cagagacgga ccagtcacca
    23581 tggcatgcgg ttgctttagc aagtagccgc tctgaggcag acatccttgc gcagcgcctg
    23641 gctcagcttc cggaggtcag caaagttgtt aacctgaccg attttgtgcc aagggagcag
    23701 gcggagaaac ttcccctgat cgaggaaatg gcgcttacga tgggtcccat caccatatct
    23761 agcggaaagc gcgcggcgga tgatgcgcct gctgatcagc tgaagtcgct tgaaaagctc
    23821 gcaagcaccc tggatcggtt cattgccgac cgtcctgatc accccgccgc tcagccggca
    23881 cgtcgcctca gatcatctct cgacaaattg cttgctcaat tgaattcctc cagtgcggat
    23941 gaaaaacaga atctgttgag cgcggtggag gatgatcttc tcgcggctct gcccattgcg
    24001 ttgcgcaatt tacagactgc tacagaagct gctcctttca acgagcaaac gcttcccgaa
    24061 tctttaagac aacggtggcg cagccaggcg ggagagtacc ggcttgcaat ctatccggct
    24121 gaagatctta acgataatga cgcgttacga cgctatgttc gcgcggtaca aggcgtggcg
    24181 ccggacgcga cgggcgcgcc aatggttagc cttgaagcag gcgaagctgt cgtacgcgca
    24241 ttcgcccagg cttttatgct ggcccttgcg ggcatcgtgg tagcactgat gattgtatta
    24301 cgaagcatca agtacaccgt gctcgtgctg attccgttac tattatccag cctttttaca
    24361 gctgcgttta ctgtcctgct gaacgtaccc ttcaattttg caaatattat tgccttgcca
    24421 ctgctgcttg gccttggaat cgacagcagc cttcacatgg ttcacagaag ccttaaccat
    24481 gagcttgtca gtgaggtgct gattcacacc agcaccgctc gcgcaatttt ttacagcgcc
    24541 ctcacggctc tggttgattt cgccagtctt atgctttcct ctcaccgagg aaccgccagc
    24601 atgggaatac tactaacggt tggtctggct ttcaccttga tctgtacgct cgtcatactt
    24661 ccggtattgc ttaacgctcc aactcgcacc cccatcggag tgcctcgtac agctggacgc
    24721 aaagatgcgg ggtgatggaa aaattttggt acttcgaggc ctggaagctc taatggaaca
    24781 gccgataaag tgtgggcgaa tccaacaatt attgtttata attcgcacct tgagaccact
    24841 catcagatcg gggaaattga aagaactgaa taatctgtct caacaaagcc caatcggctt
    24901 actcctaccc acatgaagtg tatcgcgaca gtctctttat tgcttgtatg cacgtttctg
    24961 gcaacgggtt gcgcaaccat gcgcccgagc gaggggagcg cggaaaccgg taaatcggaa
    25021 gccgacgagc agatcgatcc caacgaggaa accaaccgca agttttacga atttaccgac
    25081 atgatcgacc ggaatgtagt agccccggta gcggatgttt acataaagta cgtccctggt
    25141 cccatgcagc gatccatcgg gaatttttac gacaatcttg cttatccgaa tgttattctc
    25201 aacgactttt tgcaggggaa agtacgacag ggatttgagg atagcctgcg tttcgtggtg
    25261 aactcgaccg caggcgcagc ggggcttttt gacgtggcaa ctccactggg cctgcctcaa
    25321 catgatgaag actttggtca aacgctgggc gtatggggag tgggaacggg ttcgtatctt
    25381 ttcgttccgt tgattgggcc aagcagttat cgggacgcac caggccttcc tgtaagcctt
    25441 ttcactaacg tcctgtttta tgccggaaca tttgccagcg gcgcgtttac agccccgata
    25501 acggtactca gcattatcga caggcgcgcc cgactggccg ggccaatgcg tgttcgcgac
    25561 caagcggcat tggatccata tctttttgtc cgcgaaggtt ttttgcagca gcgcaaacac
    25621 ctgatctacg acggtgaccc acctcccgaa agctttgatg aagcaatcga ggaggagcta
    25681 tcggacgagc aggcaaatgg aaaaacccgg aaggcaaact gaccctgttt ccggggattg
    25741 tttcttcata tgaaaaacga gaaggagttc atctttcttc ccgcaactca atggatgacg
    25801 ttttgtatga gcgagaggac tggctggtta acttgcttga gggggcaagg taaatagaaa
    25861 tgaaaagatc aaaaactgta gcaaggttgg ttccgaacga tcgattcacg agttcgacaa
    25921 acacgaagac caacaggacg tcgccggaag atgggttggc ctcagcacga aaggcactgc
    25981 tgtccttgca aaaggatgac ggtcactggt gctttccctt ggaggctgat tgcacgattc
    26041 cgtccgagta cgttctgatg atgcatttca tggatgatgt ggacgttgct ctcgagcgaa
    26101 gaattgccca attcatccgc gaaaaacagg atgcggggca tggcgggtgg tccctttatt
    26161 ttgggggtga ctttgatttg agctgtacca tcaagtcgta ttatgccctt aaaattgttg
    26221 gagactctcc cgacgccccc catatggcgc gggcaagggc cgctattctg agccacggtg
    26281 gagctgcccg cgccaatgta tttacccgcc tgatgctcgc catgttcggc cagattccct
    26341 ggcgcggcgt tccgttcgtg ccggtggaaa tcatgttgct gcccaaatgg ttcccgtttc
    26401 atttgagcaa ggtttcctac tggtcacgcg ctgtgaccgt gccactatca attttatgta
    26461 gcctgaaggc gcgtgcagta aatccccgta acgtcggaat acgcgaactg tttacggtcc
    26521 cccctgagga ggagcgggac tattttccaa cccgaacccg cctcaaccgg ctgctcctga
    26581 tgttggaacg cacgggggcg tacttcgaac cctttattcc tggccccctg cggcggcttg
    26641 cgcttcgtcg ggcggaacag tggacaatcg agcgtttgaa cggagagtgt gggttgggag
    26701 cgatttttcc agccatcgtc aacgcatacg aagcgctggc cctgcttgga tatccttacg
    26761 aacaccctta ccgggagcaa tgccggcggg ccctccttgg actcgtgatt gaagagggtg
    26821 agcgggcgtg ggtacagccc tgcacctcgc cggtctggga cacggtgctc gcatcccttg
    26881 ccttgcagga agaccccgag gtggataaga aagcggtagc gcgcgctctc gattggctga
    26941 ttcccaacca ggtgctggac gcgcctgccg attggaagga tgagcacccg gggctggcag
    27001 gtggtggatg ggcgttccaa tatgccaatc cccattaccc tgatctggat gacacggcgg
    27061 ctgctgcctg ggcgctatac cagggagacc ccgaagctta tcgggagagc ctggttcgcg
    27121 cagcggactg gctggctgga atgcaatcta gaaatggcgg ttttgcagct tttgacagcg
    27181 acaataccta ttactacctc aatgagattc cttttgcgga ccatggagcc ctgctcgacc
    27241 cgcccacaag cgatgtgaca gcgcgttgcg ccggctttct ggctttgtac ggcgagtcca
    27301 ggcacaagca gaacgtggag agaggactgg agttcctttt ccgcgaccag gagccgagcg
    27361 gcgcctggtt tggacgctgg ggcagcaatt atatttatgg tacctggtca gtactggagg
    27421 gcttgcgtct cattggcctg gataccggcc acttcgctat ccgccgagct gtacagtggc
    27481 ttaagtcggt acaacgtgat gacggtggct ggggggaaag caactacacg tactccgcgc
    27541 cggaacaagc cgggcggttc cactgcagca cagcgttcca gactgcctgg gcagtactcg
    27601 cgctcatggc agctggcgag gggcgtagcc acgaagtgcg ccgtggaata gaatacttgt
    27661 tgtacgaaca gagcgaagat ggcttatggg acgatcaatg gttcacggct cccggcttcc
    27721 caaaggtttt ctacctcaaa taccacgggt ataacaaata ttttccggta tgggctctgg
    27781 cgaggtttaa cgcgataaac cggaaaatat cggggtgagg gaaataggta ttgtcacggc
    27841 aatgcgcatg gaggcgcgct gcataacacg caggcgcctg cctttcaata aaagagtggt
    27901 tttgggcgaa cgcacggcga tatggctttg cggaatgggt aacgaggcgg cccgcaaagc
    27961 tgccgagggg cttaaagcaa gtggcgcttc ggccttggtg agttttgggt ttgcaggagc
    28021 actggattcc aatttgcgcc cgggagatct cgtgctgcct gataccattc atgggcggca
    28081 atccctgccc gtggacccgg gctggagatc tcgcctgcgt aaatgccttc ccgcttcact
    28141 gaatgttctc agtggagtgc tggctacaag cgatatagtg ctgtgttctg caaacgccaa
    28201 gcgcgagttt ggccgcgcta cgggcgcctg cgcagtcgat atggaaagtg gtcctgtggc
    28261 ggaggcagca gcggttgccc gaatcccctt cctggctgtg cgcgttatct cggacgcagt
    28321 tgaattttcg ccgccacctg ttttgctgaa cgctatccgc cccgacggcg gagcggatct
    28381 gtcgcggctg ctacctttgc tgttgcgcgg cgccgtaacg ccgtcaaccc tgctcaggct
    28441 cgcgaaggag tcacgcgtcg cctgcgccac cttgtccagt gtagccaggc acgctgaagc
    28501 cgagctggga atcgcctgac aaccttccct gaaaaccttc cctatctgta ggtggttcca
    28561 ctggctgtcc tgcgactttt ccatttccac gtaaaacttg ttcccgcatt ccgtaacgag
    28621 taaaatatcg ccctttcgca tcaggcattt aatggagagc acatgccagt aacaacggat
    28681 caaaaagcgc aagtggtgcg cgagtatcaa cgagccgccg gagatacggg ttctcccgaa
    28741 gtacaagttg cattactgac ggcgagaatc aatgacttgg cgaatcactt caaggcgcac
    28801 gtgaaggatc atcattcgcg ccgtggcctg ctgcgcatgg taagcaggcg tcgcaagctg
    28861 ctcgattact tgaaacaaag cagcgcagac cgatatcgcg cacttattga gcgccttggc
    28921 ttacggaagt agaaatatct attgcccttc agcggagctg tcagcacagc aagcagaaat
    28981 ccattcgatc ggactgttta gccaagctct cagtctcact cctgcttttg gccgagacat
    29041 acgaatgctt attgaaaagg gtattaattg aattcgataa agaaaaccat ttcatatggg
    29101 gctcatcagc ttacatttga aaccggagag atcgcaaggc aggcccacgg agcagtaatg
    29161 gtatcgctgg atgataccgt tgtcctggtt actgtcgtgg gtgcgaagac tgcgaagcag
    29221 ggtcaggatt tcttcccgct gaccgtggat taccaggagc gtacctacgc ggcgggcaag
    29281 attcccggaa gcttcttcaa gcgcgaaggc cgtccgtctg aaaaggaaat cctgacctcc
    29341 cgcctcatcg atcgcccgat ccgcccgctg ttccccgaaa atttctacaa cgaagtacaa
    29401 attgtcgcaa cggtcctgtc gtctgatagc gaagttgacg ccgacatacc tgcaatgctt
    29461 ggagcgtctg ccgcgctcgt tctttcgggt ataccgtttg cgggccccat cggttccgcc
    29521 cgcgtcgcgt acataaatgg cgaatacgtt ctgaatccta ctacgagtga gcttaggcaa
    29581 acgcaactta acctcgtcgt tgccggaacg cagcgggctg ttctgatggt ggaatccgag
    29641 gcttccgaat tatcggaaga cgtcatgctg ggcgcaatca cctacggcca tcaacaaatg
    29701 cagtctgcga tcgatgccat taattcgctc gctgatgaag caggagtcac ggcgtgggat
    29761 tgggtacctc cccagagaga caccgaactc gccacccgga ttgcagagct tgcggaagca
    29821 gatctgcgtg ctgcttttca catgaagcag aaacaggcgc gttccgaagc catagatagt
    29881 atctggacaa aagttttcca tgaacttagt gttgacgccg agggtggacc ggatgctcag
    29941 gctgtaaaag aagcctgttt tgcgctggaa tcaagaatcg tgcgctctca aattctcgat
    30001 ggcgagccgc gcatcgacgg acgagatacg cgcacggttc gtcctattac gatacgccat
    30061 ggcttgctgc cacgcacgca cgggtcggcg ctttttaccc gtggagaaac tcaagcattg
    30121 gtcgtggcta cgctcggcac aggacgagac gagcagaaaa ttgatgcgct gcaaggagac
    30181 tactccgagc gcttcatgct tcactataac atgccggcct atgccacagg agaaacgggc
    30241 cgggtaggca cgccgaagcg ccgtgaaatc gggcatggac gtcttgccaa gcgagccctc
    30301 gttgccgtac tgcctactcc cgaggagttt ggttattcga tgagggtggt gtctgaaatt
    30361 accgaatcga acggctcgag ttccatggcc tccgtttgcg gcggatgcct tgcactgatg
    30421 gatgccggag tgcctttgaa agcgcatgtg gcaggtattg ccatgggcct tattaaagaa
    30481 ggtaaccggt ttgccgtcct gactgatatt ctcggcgacg aggatcatct gggtgatatg
    30541 gatttcaaag tggcggggac ggaggccggc atcactgcgc tccagatgga tatcaaaatc
    30601 cagggaatca ctaaagagat tttacatgca gccttgattc aggcgaaaga aggacgcatg
    30661 catatcctca agatcatgaa ggatgcgttg ccggttactc ggtcggatat ttccgaacat
    30721 gctccccgca taatcaagat aaagatcaat ccggagaaaa tacgcgatgt aatcggaaaa
    30781 ggcggggcgg taattcgcgc tctgaccgag gaaacgggca ccacgatcga cattaccgac
    30841 gacggtactg tcatgattgc atgtgtcaat gccgaaggcg gagagctggc aaaaaagcgc
    30901 atcgaagaca ttactgcaga ggtcgaagtc ggccgggtct atgatggaac agttttaaaa
    30961 ctgctcgact tcggggcaat tgtcagcgtc ctgccgggca aggatggctt gctgcatatc
    31021 tcgcaaattg cgaacgagcg ggtcaataac gtggctgatc atttgaagga gggccaggtc
    31081 gtccgggtaa aagttctcga agccgatgat aaggggcggc tgcgactgag catgaaagcc
    31141 gtgaatgcgg agacggcgga cgatactgct tcctagcttg ctccatagcg aaaacgttaa
    31201 aaaaccccgg cctcacaacc ggggtttctt ttttgatgga actagagtgt tactgccgtt
    31261 atgtcactgc cacttcactc aaggagaagg ctgagaacgg tgaaggaggt gtaagggagt
    31321 ttaaggtcct ttgactattt gagtctttgg actatttggc cacctgcttc tcccttattt
    31381 catcaagcgt cttgcagtct atgcatagtg tggccgtagg acgagcttcc agccgtttaa
    31441 ggccaatttc cacgccacag ctttcgcagt aaccgtaatc gccggtatct atttttgcaa
    31501 ttgtctcctc aatttttttg atcagcttgc gctcgcgatc ccggtttctc agttccagcg
    31561 ccatgtcgga ctcctggctc gctcggtcat taggatcggc aaaaattgtc gcctcatcct
    31621 gcatggtatg cacggtgcga tcaatgtcgt gccccaactc aactttaatg ccttccagta
    31681 tgtttcggaa atgcataagc tgcttggaac tcatgtaact ttcccccttc tttggcttgt
    31741 aaggagcaac ttgtttttgc agttctggcg gcatatcgat attcctttat tcctggtgac
    31801 gtcaaactaa attagacctt gacaaacatt atacagatta ctcaaaggca cttcggggta
    31861 cttcgatatt catccgcgac gtcccaaaag gaattgaaac tcctcgtcaa agccgagaag
    31921 ttcccgaggt cccgggctcg tggatagcag cgtgaatttt tgcagaattc tcatcgcttg
    31981 aataccccta cagcagaaat aaaaagaagc tctaatctca aacgaaacgg atgacaagcc
    32041 ccgggcgaag gtcaatcccg aatggctcta tccttgaatt gtgacataat attggcccga
    32101 aattaaggag agcattatgc gaagcaagaa atcgatgctg ggcattgccg ttggctctgc
    32161 actcactgcg ggcattcacg tcgcgcctgc ttctgcagcc gacgaactat tcagtgtacg
    32221 ggaactggat aagggataca tggttgcgta tgccgaccgt gttgatccgg acaaatacgg
    32281 gggaggaaaa ccaggcggat acggaggcgg ttcgggtgga gggaagacgg cggaggggca
    32341 atgcgggatg tcgcgaatag acgtcaacaa ggatggcaag gtaacgaagc aggagtttct
    32401 caagcaccat gaaactattt tcgaccagat tgacgcgaac aaggatggcg cggtcgacca
    32461 acccgaggcc gatgggtacg ccgcgaaggt gaacgccgct tcaaatccgg ctccccatgg
    32521 ggcgatgaaa aaataacaaa cgagcttctc cgtgagcggc gctcgcctca ttgaaggtac
    32581 cgggttgggt ttgcggcggg agttgattcc cgcactgcaa tctggtgttc ctgcagcgat
    32641 tgactttttt gaaatcgctc cggaaaactg gataggtctg ggtggcaagg cggagcggga
    32701 tttaagagcg cttacagagc agtttccctt tgtctgtcat ggactttcac tatcgatcgg
    32761 ggcccctgcg cggttaaatg aggacctgct gcgggggatc aaaaaatttc ttgatctcca
    32821 caacgtttcc ctttatacgg agcacctcgc gtattcttcc gacgaggggc atttatacga
    32881 cttgcttccg attcccttta caggggaagc ggtgaaatat gttgcttcgc gggtgcgccg
    32941 cgcccaggac gttctggagc ggcgtattgc cctggaaaat gcatcgtatt acgtccaggc
    33001 gcctatttca gagatgagcg aggccgattt tatccgtgcc gtgctcgtcg aagctgactg
    33061 cgatctgcac ctcgacgtca acaatgtata cgtcaacagc gttaatcatg cctatgacgc
    33121 tgaggaattc atccacagca tgcccagaga gcgcatagtc t
//
LOCUS       DASSHJ010000178        10507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1000040,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000178 DASSHJ010000000
VERSION     DASSHJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000040"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VFI43_06940"
     CDS             complement(<1..1190)
                     /locus_tag="VFI43_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET7061898.1"
                     /translation="MNRFLFCTGIENSYPTIQLPNGEMTRVDEMEKCGHYERWREDFD
                     RVLEMGLEYLRYGPPYYRTHLGPGNYDWSFSDETFNELKRLNIDPIVDLCHFGVPDWI
                     GNFQNTEWPALFAEYARAFAVRYPWVRFYTPVNEIYVCATFSGQWGMWNERLLSDSAF
                     VTALGNLCKATLLAERAILEIRPDARFVQSESSEYFHPEDPGAQEIAEIFNEKRFLSL
                     DLCYGKDVSARMYQYLTDHGMSREDYAWFQKTGHTLRPHCIMGNDYYITNERLVSRTG
                     RLSHAGEVFGFYVIARQYFDRYHLPVMHTETNLGDAEKAPGWLWKEWANAIRLKQDGV
                     PIIGFTWYSLTDQVDWDTALCENNGRVNPLGLYDLNRKIRPVGVQYRRLILQWGQALP
                     AERI"
     gene            1582..2331
                     /locus_tag="VFI43_06945"
     CDS             1582..2331
                     /locus_tag="VFI43_06945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HET7061899.1"
                     /translation="MLEQDKKPELIDDEKYRISSKAGVLAVLKLMIRNKSLATCAFGD
                     RDNFFPTALLGLDVRRDEMVLDYGPDEGINRQALKADKWNIVAFPNQVKIHFSCQQIR
                     KIELEGRSAFLTEIPKSLLRVQKREYYRVVIPAMAPVKCVIPLLDPEPPVPVQVILEN
                     ISCGGMTFIDREYRTDFEIGTVYENCLVTLPGVGTAKASIRIQRVEPGHASALAYQRV
                     SCEFIDTEENRLSLIQRYVIKLELERKRNFD"
     gene            complement(2518..3102)
                     /locus_tag="VFI43_06950"
     CDS             complement(2518..3102)
                     /locus_tag="VFI43_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7061900.1"
                     /translation="MKNLNLKLLAVAFFLLPLPLLSGCALVAATGVAGTGVAIAEDRR
                     TSGTMVQDESIEVKSNRRIKEKFGYKVHVEVTSFNRHVLLTGEATSAKVKNEIEDIVK
                     TVEGVQDVTNEIAVGEIRPLTARSHDALITSKVKAKFVNEGRFQANHVKVVTEDGVVY
                     LLGLVDREEAQNAVKIAKSTSGVRKVVQVFEYLD"
     gene            complement(3859..4614)
                     /locus_tag="VFI43_06955"
     CDS             complement(3859..4614)
                     /locus_tag="VFI43_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HET7061901.1"
                     /translation="MHELIAHPSIRCAGYTLQLLPQRVLWWASKKTLLVADVHLGKGA
                     TFRALGQPLPRGGSADDLQRLAALVAQLQAKQLIVLGDFWHSRRGLNPTLFNALHQWR
                     LQHAQLQVMLVRGNHDRAISKTELPASLRIEVVEEPFPLAPGLYACHEPPLEVAGLPQ
                     PDNNGIAKMLSAPVSTNGPEAFYLAGHLHPAVSLKGKSLDSARLPCFVWQAGQLVLPA
                     FGSLAGHLMLDLNLLRRNGAKAGIVAPDAVWLV"
     gene            complement(4630..7437)
                     /locus_tag="VFI43_06960"
     CDS             complement(4630..7437)
                     /locus_tag="VFI43_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HET7061902.1"
                     /translation="MVTDDARAKRWLALRNWQPFPFQLKVWKAMSKGRSGLLHVTTGA
                     GKTLAVWLGALERFVPLDQGKEKQTGSEGANSHDVSRKGNIAPSLTVLWLTPMRALAA
                     DTLRALREPLPDFAPHWTSGLRTGDTPSGERAAQNRCLPTLLVTTPESLSLLLARADA
                     PETLATVQLVVVDEWHEMLGNKRGVQVQLAMARLSGWNPQLMVWGMSATLADLKRALS
                     TLLPLPAPAVPLKQSGKLPPAPLMPVLIKDRTAKKLVVDTLLPERVERFAWAGHMGLS
                     MLPRVGKEIAQSGSTLIFTNTRSQAERWYQALLEHYPGWAGEIALHHGSLDLSVRNWV
                     ELGLKNGTLRAVVCTSSLDLGVDFLPVERVLQVGSAKGVARMLQRAGRSGHAPGRPSR
                     ITLVPTHSLEIIEAAAVRQAIQTGQIEDRHSPNQPLDVLVQHLVTVALGGGFQPDALY
                     AEVRRTFAYAGLSPDNWEWALDFVRQGGPSLAAYPDFQRAAPDAYGIWRVGDSRLARR
                     HRSNIGTIVSDAVLVVQFMSGGRLGTVEESFGARLRPGDVFIFAGRLLQLVRIYQMTA
                     YVRLAKAGSPALPRWNGGRMPLSSTLADTMLSELDAAERDEYRSPEMRCVQPLLELQK
                     KWSHLPTPRSLLAETLKSREGWHLFLYPFAGRQVHLGLAGLMAWRAAQAQANTFSIAL
                     NDYGFELLSTKPMDWAKLLPDMLALPKERVPASFHQQQNQPQHRPQLLAEVLASLNAT
                     ELARRRFREIARISGLIFQSHPGEQRSNRQLQASASLYYDVFQNYDPGNRLLQQAKSE
                     LLQQELDIDRLARSLERMQGQALHIVAVKKPTPLGFPLMAERFREKLSNEPLAARIAR
                     MVEELEASAGSNAPASLGRKRGNQAAPASTREKILQTLELNSQADPRWKQEKDGPPET
                     NSPSGGASAMRRIRARYRRRL"
     gene            complement(7465..9129)
                     /locus_tag="VFI43_06965"
     CDS             complement(7465..9129)
                     /locus_tag="VFI43_06965"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7061903.1"
                     /translation="MKAFSALYRELDSSTSSLAKQAALQSYFRSAAPADAAWAVFFLA
                     GGKPRQMVPNRLLKALAQEVAALPEWLFVESYEAVGDLAETIALLLPDPPDTKELSLA
                     EWLSVHLLPLRGLPEEELRARLLAQWRTISAADRLVYFKLITGSLRVGVSRLQVTQAL
                     AAVSGVDAKRVAQRLMGYTQIGSQPAAVDFLALIRPEDAQEQGPQLAGHPYPFFLAHP
                     FNQPLAHFYELLGSPADWMVEWKWDGIRAQLVKRGGQSWIWSRGEELVSEQFPELAQA
                     GLSLPDGMVLDGEIVVWRNGKVQPFNELQKRLGRKIVGSKVLREQPVILLAYDLLEEE
                     GEDLRRQSQYIRRARLEALLEKHAPEASGLPLRLSPIVQGSKWEELAILREGARSQGT
                     EGFMLKQRNAAYGVGRTKDVGRWWKWKIDAMSVDAVLVYAQRGHGRRASLYSDYTFAV
                     WSDSPENPMRVLVPFAKAYSGLTDAEMAQVDAIIRKTTRESFGPVRSVEPTLLFELGF
                     EGISRSSRHKSGIAVRFPRMLRWRQDKPVAEADTLQALAALLPDGA"
     gene            complement(9129..10304)
                     /locus_tag="VFI43_06970"
     CDS             complement(9129..10304)
                     /locus_tag="VFI43_06970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HET7061904.1"
                     /translation="MTLPLIVNRPEGLYCPEGEFFIDPWRPVNRAVITHAHSDHARPG
                     NAHYLAHRDSAELLHIRLGADISLQTLEYGEALEHRGVRISLHPAGHVLGSAQVRLEC
                     RGRVWVASGDYKLADDETCQPFEPVRCDTFITESTFGLPIYRWPHQADLFVEINNWWQ
                     RNAASGRASILFCYAFGKAQRILRGVDSTIGPIVCHGAVEPLNAVYRATGVPLPGTFR
                     VTDRTMNKSALSQALVLAPPSTAGTPWLRRFGDYSDAFASGWMQLRGARRRRGVDRGF
                     VMSDHADWPGLHRAIGNTQAGQVFVTHGSIAIMVRYLREQGLDAQAFATEYGGEEEAD
                     PVNGEAIPMSDVQDSNHTGDAAFSVEVDKDGAGANNPGASGFSLDLSAARRPRPLDS"
BASE COUNT         2296 a         3036 c         2910 g         2265 t
ORIGIN      
        1 caaattctct cagccggtaa agcctggccc cactgcaaaa tcaaacgccg gtactggacc
       61 ccaacgggac gaattttccg gttcaggtcg tacaacccga gcgggttcac gcggccatta
      121 ttttcgcaaa gggcagtgtc ccagtctacc tgatcggtaa ggctatacca tgtaaaacct
      181 atgatgggca cgccgtcctg ctttaaccgg atggcgttgg cccactcttt ccatagccac
      241 cctggcgctt tttccgcatc gccgaggttg gtctccgtat gcatgacggg gagatggtag
      301 cgatcgaaat actgacgtgc aatcacataa aagccaaaca cttcgccggc atggctgagg
      361 cggcccgtcc ttgaaaccag ccgctcgttg gtaatgtaat agtcgttgcc cataatgcaa
      421 tgcggcctta atgtatggcc cgttttctgg aaccacgcgt aatcctcccg cgacatgcca
      481 tggtcagtca ggtactgata catgcgggcg ctcacatcct tgccatagca aagatcgagc
      541 gaaagaaatc ttttttcgtt gaaaatttcc gctatttcct gagcccccgg atcctcggga
      601 tggaagtatt cgctggattc gctttgcaca aaacgggcgt cgggccgaat ctccaggatt
      661 gcgcgttctg caagcagggt cgccttgcat aggttaccaa gtgcggtcac gaacgcagaa
      721 tccgacaaca ggcgctcgtt ccacattccc cactgcccag agaaggttgc gcatacataa
      781 atctcgttca caggggtata aaaacggacc catgggtaac ggacagcgaa agcacgtgca
      841 tactcggcga aaagagcggg ccattccgta ttctggaaat tgccgatcca atcaggcacc
      901 ccgaaatggc aaagatccac gatcgggtcg atgttcagcc tctttaactc gttgaaggtt
      961 tcatcagaaa acgaccagtc gtaattcccc ggccccagat gagtccgata ataaggcggt
     1021 ccatagcgca agtactccag acccatttcg agtacgcggt caaagtcttc gcgccatcgc
     1081 tcgtaatggc cgcacttctc catctcgtcg acccgcgtca tttcaccatt gggaagctgg
     1141 atagtgggat aactgttttc gatgcctgta cagaaaagga aacgattcat ttgtttattg
     1201 ccataactgt cccgacttct acgagatgct gaaaaacagg atacccatat gcgccgactt
     1261 gttgttcttg tgatctggag gtcagtccaa agtaacaacg aaattaagaa actacggctt
     1321 taagccgatg gagcccttgc ctttacagca ttgactcgct ctaacgccag tgaattcgtt
     1381 atcgcttggg ggcagttgcg agaaattctt gccagactcc tcaattcact ccaatagcgg
     1441 ccgttattag gaaattgtag cccgtgaggc tgctggcgtc tgcctgttgg ccgtctggct
     1501 tttgatgcaa caaattgcaa atttaatgga atggatagat cagtccgaac tgtttcataa
     1561 aagttgagca ggcaaaagat catgctggag caggacaaaa aacccgaact cattgacgat
     1621 gagaaatatc gcatttcgtc gaaagcgggg gttctcgccg tgctcaagct tatgatccgc
     1681 aacaaatcgc tggccacttg cgccttcggc gatagagata attttttccc caccgccctt
     1741 ttaggccttg atgtacggcg cgatgaaatg gtgctggatt acgggcccga tgaagggatt
     1801 aatcggcagg cactgaaagc cgataaatgg aacatcgtcg cgttccccaa tcaagtgaag
     1861 attcatttta gctgccagca aattcgaaag atcgagcttg aagggcggag cgccttcctt
     1921 acagagattc caaaaagcct gctgcgcgtg caaaagcgcg agtattaccg cgtagttata
     1981 ccggcaatgg cgcctgtgaa gtgcgtgatt cctttactcg atccggagcc acctgttcct
     2041 gttcaagtta ttctggaaaa tatcagttgc ggcggaatga cctttattga ccgggaatat
     2101 aggacagatt ttgagatagg aaccgtttac gaaaactgcc tggtcacgtt acctggcgtt
     2161 ggcacagcca aggcgagcat caggatccaa cgcgtggagc ctgggcacgc gagtgccctc
     2221 gcttaccagc gcgtgagctg cgaatttatc gataccgagg aaaataggct gtcgctgatc
     2281 cagcgctatg tcatcaagct ggaactggag cgaaaaagga attttgatta gaagaagggg
     2341 aaccctcgtt ttgttgggcg aaaagttcaa ttttgcacgc tattccggct ttcaaaacgg
     2401 gagctaaaaa ctgtggtctg tccccaattc ttgggtccca attccggggc gggggcgaga
     2461 ataatgccaa gtccgaatac aaattagtag gactctcccg caactaaagc tcaagtatta
     2521 atccaggtac tcgaacactt gtaccacctt gcggacgcca cttgtggatt tggcaatctt
     2581 caccgcgttt tgcgcttcct cccggtccac caaccctagc aagtagacca ccccatcctc
     2641 ggtgacaact ttaacgtggt tggcctgaaa tctcccctca ttcacgaact tcgccttgac
     2701 cttcgatgta atcaaggcgt catgactgcg cgcggtaagc ggtctgattt caccaaccgc
     2761 aatttcattc gtgacgtctt gcacaccctc tactgtcttg acgatatctt caatctcatt
     2821 tttaaccttc gcagacgtcg cttcgcccgt aagcagcaca tggcgattaa agcttgtcac
     2881 ctccacgtga accttgtatc caaacttttc cttgattctc cggttgcttt taacctcaat
     2941 actttcatcc tgaaccatgg tgccgcttgt acgtctatcc tcagcgatcg ccactccagt
     3001 gccggctaca cccgtggccg ccactagcgc gcaaccagag agaagtggaa gcggaagcag
     3061 gaaaaaagca actgccaata acttcaaatt caggtttttc atatattccg ttgaataagt
     3121 aaatgggaga gacacggcta gcagcgcgtc gcgcaatccc gctttgtagc agggcgatgt
     3181 ggctttcccg acgtgtggcc ccggaaatgg ggtgatgatg agaaaaaggt attgcgcaga
     3241 tagcggtttg ttttttggtg ggtcgtgagc gattcgaacg cttgacctag gattaagagt
     3301 cccgtgtaac caataattga gaataattgg cagcaaatta taggaataga atcaggaact
     3361 gttaacactt atttagtgac gcgttgctgt acgtaaaagc gtggggcgag gcgcgaagcg
     3421 caggacatag caagcctatg gtcaagcttt gcaacgcagc atccacgttt ttttcgtgcg
     3481 caacccgaag agacggggcg gattttaccc ggccccgtcg atgctcgccg cttagagagg
     3541 cttgctctgt ggcgcgactc gcgtctctgc tgcataaaat cgtcgccgtc gcggacgtga
     3601 gataagtgtt aacaggccct agaacgttat cgatatttct ttattgctgc tcattctgtt
     3661 ttatcgcggt attttacagg aaagttccca ctattgggct cgcggggtaa atgaatagtg
     3721 atcaaatcta cctgacgggg ccctccgata aacgattatt gacataattt aagcccgtta
     3781 cgggccgtct tgggggcatg aatctctggc agcatgaatg ggtctactgc ctgcttgtcc
     3841 ctttcgtgga cgacccacct ataccaacca gactgcatcc ggtgcaacga tcccggcctt
     3901 agccccgttc cgccgcaaca aatttagatc gagcatgagg tggcctgcca agctaccgaa
     3961 agcggggagt acaagctgac ccgcttgcca gacaaagcac ggcagccgag cactgtcgag
     4021 gctttttccc ttgaggctta ccgccggatg caagtggccg gccaagtaaa atgcttctgg
     4081 cccatttgtc gataccggcg cggacagcat cttcgcaatg ccattattgt ctggctgagg
     4141 caaacccgcc acctccaacg gaggctcgtg acaggcatag aggccgggag ccagcggaaa
     4201 aggttcttcg accacctcga ttctaaggct cgccgggagc tcggtcttgc ttattgcacg
     4261 gtcgtgattg ccacgcacca gcatcacttg cagctgcgcg tgctggaggc gccattggtg
     4321 aagcgcgttg aaaagcgtgg gattaaggcc tcgtcggcta tgccagaaat cccccagcac
     4381 aatcagctgt tttgcttgca gctgcgccac cagcgcagca agacgttgca gatcgtccgc
     4441 gctgccgccc cgcggcaagg gttgacccag cgctcgaaaa gttgcgcctt tgcccagatg
     4501 cacatcggct accagcaacg ttttttttga ggcccaccac agtactcgct gtgggagcag
     4561 ctggagagta tagccagcac agcggatgga gggatgggca atcaactcat gcatgcgagg
     4621 tcatacaact cagagacgcc gccggtatcg ggcacgtatt cttcgcatag cagacgcgcc
     4681 gccggatggg gagttggttt cgggaggacc gtccttttcc tgtttccagc gtggatcggc
     4741 ttggctattc aattcgagcg tctgcaaaat tttctcgcga gtgcttgcag gcgcagcttg
     4801 attgcctcgc ttgcgcccga ggctagctgg agcgttcgat ccggcgcttg cttcgagttc
     4861 ctccaccatg cgcgcaatgc gggcggccag cggctcattg ctgagttttt cgcgaaaacg
     4921 ttcggccata agcggaaaac ccagtggagt gggttttttt acggccacaa tgtgcagcgc
     4981 ttgcccctgc atgcgctcaa gcgatctagc caggcggtcg atatccagtt cctgttgcag
     5041 cagttccgat ttcgcctgct ggagaagacg attgccggga tcgtagttct ggaacacgtc
     5101 gtagtataac gaagcggatg cctgcaattg ccgattgctc cgttgctcgc ccgggtggct
     5161 ttgaaagatc agtccggaaa tgcgcgcaat ttcgcgaaag cgccgccgtg ccaattcggt
     5221 ggcattgagg ctggcgagca cttccgcgag gagctgcggc cggtgctgcg gctggttttg
     5281 ctgctggtga aaggaagcgg gaaccctctc cttcggcaag gcgagcatgt cgggcaacag
     5341 cttggcccag tccataggct tggtggacaa cagttcaaat ccgtagtcgt tgagcgcgat
     5401 tgaaaacgtg tttgcctgtg cctgtgcggc acgccatgcc atgagtccag ccaatcccag
     5461 atgcacctga cgccccgcga acggataaag aaacaagtgc cagccttccc ggcttttcag
     5521 cgtttcggcc agcaacgatc gcggcgtagg aaggtgcgac cacttttttt gtagttccag
     5581 caagggctgc acgcagcgca tctcggggct gcggtactcg tctcgctcgg cagcgtcaag
     5641 ttcgcttagc atggtatcgg caagggtgct ggacagtggc atgcggccgc cattccagcg
     5701 cggcagcgct gggctgcctg ccttcgccaa cctgacatag gctgtcatct ggtagatgcg
     5761 taccaactga agaagacggc cggcaaatat gaacacgtcc cctggccgca gtcttgcccc
     5821 aaagctttct tccacagtgc ccaggcggcc gccgctcatg aattgcacca ccagcactgc
     5881 gtcactcaca atagtgccga tattgctgcg gtggcgccgc gccagccgtg aatcacccac
     5941 ccgccagatg ccataagcgt cgggcgcggc acgctggaag tccggatagg cagccagcga
     6001 aggaccgccc tggcgtacga agtccaacgc ccattcccag ttatccggcg acaagcccgc
     6061 ataggcgaag gtgcggcgaa cttcagcata caatgcgtcc ggctggaacc cgccacccag
     6121 cgccaccgtt accaagtgct gcaccagtac atccaggggt tgattgggac tgtggcgatc
     6181 ttcgatctgg ccggtttgaa tggcttggcg cactgccgcc gcctcgatta tttccaagct
     6241 atgggtaggc accagcgtga tgcgggaagg ccttcccggc gcatgtccgg accgtccggc
     6301 gcgctgcagc atcctcgcca cgcccttggc cgagccgact tgcagcacgc gctcgacagg
     6361 caggaagtcg acgcctagat cgaggctgga agtgcaaacg acagcacgca aggtgccatt
     6421 cttcaggccc agttccaccc aattccgcac ggataagtca agagacccat ggtgcagcgc
     6481 gatctctccc gcccagcccg gataatgctc cagcagcgcc tggtaccagc gctccgcctg
     6541 cgagcgggta ttggtgaata tcaaggttga accgctttgt gcaatctcct ttcccacgcg
     6601 aggaagcatg gacaagccca tgtgcccggc ccaggcaaag cgctcgacac gctcgggcag
     6661 gagcgtatcc accaccagtt tcttagcggt acggtccttt atcaacactg gcattaatgg
     6721 cgcgggaggc agcttgcccg actgtttgag ggggacggct ggcgcaggca ggggcagcag
     6781 cgtgctcagc gcccgcttca ggtcagccaa cgttgccgac atcccccata ccatgagctg
     6841 cgggttccaa cccgatagcc gcgccatggc gagctgcacc tgtacgccgc gcttgttgcc
     6901 cagcatttcg tgccactcat ccaccaccac cagctgcaca gttgcaaggg tctcaggcgc
     6961 atcggcgcga gccagcaaaa gagacaggct ttcgggcgtg gtgaccagaa gggtggggag
     7021 gcagcggttt tgagcggcac gctctccgct cggcgtatcg ccggtacgta atccggaggt
     7081 ccagtgcgga gcgaaatcgg gcagtggctc gcggagggcg cgcaacgtat cagcggccag
     7141 tgcccgcatg ggagtcagcc agagcacggt caatgaaggg gctatatttc cttttctaga
     7201 aacgtcatgg gaatttgcgc cctccgaacc ggtttgcttc tcctttccct gatcgagcgg
     7261 cacgaatcgc tccaatgctc ccagccagac agcaagcgtc ttgcccgctc ccgtggtgac
     7321 atgcaggagc cctgagcggc cctttgacat cgccttccaa accttgagct gaaatggaaa
     7381 aggctgccag ttgcgcagcg caagccatcg cttcgcacgc gcatcgtctg tgaccatcgc
     7441 ttcaggtcca tggcttggca atgctcaggc cccgtccggg agcaacgccg ctagcgcctg
     7501 cagtgtgtcc gcctcagcga ccggcttgtc ctggcgccag cgcagcattc gcggaaagcg
     7561 caccgcgatg ccgcttttat gacggctgct gcgcgaaatt ccttcgaaac cgagctcaaa
     7621 tagcagtgtc ggctccacgc tgcgtacggg gccgaagctt tcacgcgtgg ttttgcgaat
     7681 aatcgcatca acctgcgcca tctcggcgtc cgtcaggccc gaataggcct ttgcaaacgg
     7741 cacgagaacg cgcattgggt tttcaggcga atcgctccat acagcgaaag tatagtcgct
     7801 gtacaggctg gcgcgccggc catggcctcg ctgcgcataa accagcaccg catccacgct
     7861 catcgcatca atcttccatt tccaccacct gccgacgtct ttggtgcgtc ccaccccata
     7921 cgcggcattg cgctgcttga gcatgaaacc ttccgtgccc tggcttcgcg cgccttcccg
     7981 caggatagcc aactcctccc atttgctgcc ctggacaatg ggactcaagc gtaatggcaa
     8041 tcccgatgcc tcgggcgcat gcttttccag cagcgcctca aggcgcgcgc ggcgtatgta
     8101 ttgcgactgc ctgcgcagat cctccccctc ctcttccagg aggtcgtatg ccaggaggat
     8161 taccggctgc tcccgcagaa ccttgctgcc cacgatcttg cgcccgagtc gcttttgcag
     8221 ttcattgaaa ggctggactt ttccattacg ccatacgacg atctctccat caaggaccat
     8281 cccgtcgggc agggacaggc ccgcctgggc cagttcggga aattgctcgc tgacaagttc
     8341 ttccccgcgc gaccatatcc aggactgacc gccccgcttg actaattgtg cgcggatacc
     8401 atcccatttc cactccacca tccagtcggc gggggaaccg agcagttcgt aaaaatgtgc
     8461 aagtggctga ttgaagggat gcgcaagaaa aaaggggtag gggtggccgg cgagttgtgg
     8521 gccttgttcc tgggcatctt cagggcggat cagcgccaga aaatcgacag cggccggttg
     8581 cgaaccaatc tgcgtgtagc ccatcagccg ctgcgccacg cgttttgcat cgacaccact
     8641 gaccgccgcc aatgcctgtg tcacctggag tcgggagacc ccgacacgca gactgcctgt
     8701 gatcagcttg aaatacacca ggcgatccgc tgcggaaatg gttcgccatt gcgccagcag
     8761 gcgcgcgcgc agctcttctt ccggcaaacc gcgcaggggt agcaggtgta cggacagcca
     8821 ttcggccagg ctgagctcct tcgtgtccgg tgggtcgggc aacagcaggg cgatggtttc
     8881 ggcaaggtcg ccaacggctt cgtagctttc cacgaacagc cattccggca gtgcggcaac
     8941 ctcttgcgcc agcgctttga gaagccggtt tggcaccatc tggcggggtt tgcccccggc
     9001 aaggaagaat accgcccaag ccgcgtccgc cggggcagcg ctccgaaaat agctttgcag
     9061 cgctgcctgt ttcgctaggc ttgaagtcga actgtcaagc tcgcgataca gtgcggaaaa
     9121 agctttcatc agctatccaa cggtcgcggg cgtcgcgctg ccgataaatc taggctaaaa
     9181 cccgaagcgc ctggattatt ggccccagcc ccatccttat ctacctcgac ggaaaacgct
     9241 gcatcgccgg tatgatttga atcctgcacg tcagacatcg gaatagcttc accatttacg
     9301 ggatcggcct cctcttcgcc gccgtattcc gtggcaaagg cttgcgcgtc gaggccctgc
     9361 tcgcgcaggt agcggaccat gatagcaatg ctgccatgcg ttacaaagac ctgcccggcc
     9421 tgggtattgc cgatagcgcg atgcaggcct ggccagtcgg cgtggtcgct catgacgaaa
     9481 ccgcgatcca ccccgcggcg gcggcgcgca ccgcgcagct gcatccagcc actggcaaac
     9541 gcatcggaat agtcgccaaa gcgtcgcagc cagggtgttc ccgcggtcga aggcggcgcc
     9601 agcaccagcg cctgggaaag cgccgatttg ttcattgtcc tgtccgtcac gcggaatgtg
     9661 ccagggagcg ggacgccggt ggcacggtaa acggcattaa gcggttccac tgcgccgtga
     9721 cagacgatag gaccgatagt gctatccacg ccgcgcaaaa tgcgttgcgc cttgccaaat
     9781 gcgtagcaga aaaggatcga agctcggcct gacgccgcat tccgctgcca ccagttgttg
     9841 atctcgacaa agagatcggc ttgatgcggc cagcggtaga tggggagccc gaatgtcgat
     9901 tcggtgatga aggtatcgca gcgcactggt tcgaaaggct ggcaggtttc atcgtctgcc
     9961 agcttgtagt cgcccgaagc gacccatacc cttcccctgc actcgagcct gacctgtgcc
    10021 gatcccagga catgcccggc gggatgcagg ctgatgcgaa cgcccctgtg ttccagcgct
    10081 tcaccatact ccagcgtttg gaggctgata tctgcaccga gacggatgtg cagcaactcg
    10141 gcactgtcgc gatgcgccag ataatgtgca ttcccgggcc gcgcatgatc gctgtgagcg
    10201 tgcgtgatta cagcacggtt aaccgggcgc caggggtcga tgaaaaactc gccctcgggg
    10261 cagtacaagc cttcaggacg gtttacgatt aagggaagtg tcatccaaaa gttggagagg
    10321 tttcatcgaa acaagttttc ataatttcaa taaccccgac agcgaaaagc gaatatcgct
    10381 tttcataacc agttatgggt cgtaacgcta tttttttcaa gacctcttga tgagcagcgg
    10441 cgatccgagc caagctttca gttcgttagg tgtcagaaat aacctcagga tgcgaacagt
    10501 cacttca
//
LOCUS       DASSHJ010000179         8568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_656066, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000179 DASSHJ010000000
VERSION     DASSHJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656066"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VFI43_06975"
     CDS             <1..1473
                     /locus_tag="VFI43_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061905.1"
                     /translation="QKMIDNCLQDILRIWDKDKNDPWFPVAAQVELSGDDHMDGKNFI
                     NVLQGLGSFEYKNVTVLFALIRCFLMTNPTRLRFIRKSYRGICEPMSGSYAWIWHRIA
                     FSDANFFEHLLVFLASDASRRHHYAHIIPILENLLGYCVCISQEWLETPNNSIPHPAI
                     TCLPVDAEGRPLCRLSEKAMQQKRDLGFGDYIPDTDTTFLTLGMARKWLDFVQREELA
                     VDKALLEACESLLAHPWVEIISEYQVGGKYSSNPPTIQITKPLDYEGAVPIWFDKTFR
                     NDGRIIREMLGNEICPGHNMDILDAILVNRAQWSALEGENLVFLQRLLDFHYQAFASR
                     NFRHETAFKYYLPEIYVYYLGRMYRTYGTLADIDKRILDPGGKIEDMRRIALRYCKDE
                     LMGYSLNAFDAALAVSALVLLAYEPRRDGVIAAGLNVLKQAMGEGRGRHPYRAYEWNR
                     MRHPTRIVVGSEVATSLFVLRACTEAMRYLEKEEFINQGT"
     gene            2187..4040
                     /locus_tag="VFI43_06980"
     CDS             2187..4040
                     /locus_tag="VFI43_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HET7061906.1"
                     /translation="MNVFYEEEGTFKVGAILADNTTSLQVEAPHGRRAKIKAASVLLR
                     FDTPSLSEFMDVAVKTAEELDPNFLWECCESEAEFASETLASDYFGHAARPEEAAAVL
                     IRLHSAPIYFYKRGRGRYKAAPSKALEAALASQEKRRRQAEQQAHYAQLLCAFSLPEE
                     FKPILPSLLYRPDKNTIEWKALEAACSTTKLTVRKLLEMCGGIPSTHDYHLDRFLLEH
                     FPEGPGFGEVDTRELMELPELPVADVTAFSIDDVTTTEIDDAFSVTPLSLGSFRVGIH
                     IAIPTLGITPGSPLDRIAGRRLSTVYLPGSKITMLPDAVIQHYTLREGGVCPALSMYL
                     DVADDFTITGTTSRVDQINVAANLRHDTLEECFNESTLAAGSVNHHFGNELLALWKFA
                     CKLEANRGKANDSNNDRMDYSFSVKDDRITISQRRRGSPIDKVVSELMIHVNAEWGKQ
                     LADGGVTGIYRSQGGGKVRMSTSPAPHQGLGVSQYAWISSPMRRYVDFINQRQLVALL
                     RGETPPYARESEDLQTYMRDFESAYEVYGDFQRSMERYWCLRWLLQEKIEVTSAQVLK
                     ENLVKLDSLPLVTRVCGLPDIAPETSIQVEISNIDLLELGFTARFLHKLHG"
     gene            4262..5194
                     /locus_tag="VFI43_06985"
     CDS             4262..5194
                     /locus_tag="VFI43_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7061907.1"
                     /translation="MNLASDISVLRPAAGPDWLLSPFRLNVAIAVSLVIHVVLLFGVN
                     FKLPFTGKELSQPLEVVLVNSKSATKPRGADALAQANLDGGGNTDSNRQNKARSRPAA
                     SPDKGIAAAELRESTERVEQLEHEATRLMTATHGAAEVSQPVAEVSSQAEQAAPAHYT
                     ADARDLVQRGLEIARLEAQTSLDYEAYQKRPKRKFIGARTKEYRFARYVEDWRFKVER
                     VGNLNYPEAARRDKLYGSLQLTVGIRSDGSLESVELNRSSGKKVLDEAAIRIVNLAGL
                     DGFAPFPPEISRDTDILHITRTWVFTRSDELMSE"
     gene            5219..6037
                     /gene="aroE"
                     /locus_tag="VFI43_06990"
     CDS             5219..6037
                     /gene="aroE"
                     /locus_tag="VFI43_06990"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7061908.1"
                     /translation="MPDRYAVIGNPVAQSKSPLIHAEFSLQTGEDVCYEAILAPLDRF
                     AQTVTDFREKGGKGLNVTVPFKLDAFRISDQVTGRARVAGAVNTLVFEGPDIVGDNTD
                     GVGLVRDIESNLGFLLPGKRILLIGAGGAARGAIMPLLGREPAALVIANRTLQKARDL
                     QQNFSGDGNILAVTYHDLQGEHFDLVINATSTSLFGELPPLPEHIFAPQSLAYDMMYG
                     KGRTPFLQFAKLQGAEYLADGMGMLVEQAAESFLVWRGVRPRTDAIIEMLGNCV"
     gene            6065..6763
                     /gene="mtgA"
                     /locus_tag="VFI43_06995"
     CDS             6065..6763
                     /gene="mtgA"
                     /locus_tag="VFI43_06995"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HET7061909.1"
                     /translation="MKRFLWRSFWRISFFLLIAGLIYHLWILGHVFYWSYYNPSSTAF
                     MEARLEVLRQKDGAARLRKQWISYERISPHLKRAIIAAEDSRFLEHEGFDFEAIQKAY
                     EKNLRKGRLVAGGSTISQQLAKNLFLSDEKTPWRKIEEALITLMLENIMTKRRIMEIY
                     LNVIEWGEGVFGADAAIRHYYGTSSSSISPEQAARLAAMVPNPRFYDRHRNTPWLSKK
                     TGIILSRMGQVQIP"
     gene            6922..8358
                     /gene="mgtE"
                     /locus_tag="VFI43_07000"
     CDS             6922..8358
                     /gene="mgtE"
                     /locus_tag="VFI43_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7061910.1"
                     /translation="MTKANKLGEAENLQEHLQRVLYLLSQRKLAQELAEHIPEQQHER
                     ELEQKQNLAELQALLDRLHPADVAHILEALPLQDRLTIWDLVKAERDGEILLEVSDAV
                     RETLIASMNSGEMLAAAEQLDADEIADIAPDLPRYVIDDVFRSLPIEEREKLRAAMSY
                     PEDAVGALMDFDIVTIREDVTLEVVLRYLRRLDELPDHTDLLFVVDRTERLKGVLPLN
                     RLLVSDPALKVGSIMSKEMVVFHPSEKAHEAAQAFERYDLVSAPVVNGEEKLVGRVTV
                     NAVMDFIREESASEALSLGGLRQEEDLFAPIWKSLKNRWTWLAVNLITAFIASRVIGI
                     FENSIEKLVALAALMPIIAGIGGNSGNQTITMIVRALALGQINAANARNLVIKEIGVS
                     VVNGLVWGGVVGLFAFVIYRSVPLGLVMMLAMILNLILAALSGVLIPLTLYRFGRDPA
                     AGSSVMITAVTDSGGFFIFLGLATLFLL"
     gene            complement(8339..>8568)
                     /locus_tag="VFI43_07005"
     CDS             complement(8339..>8568)
                     /locus_tag="VFI43_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061911.1"
                     /translation="GDLLAIMSAGAYGMSMSSNYNTRARAAEVMIDGDRFHVIRQRET
                     MEQLMAGEVIPLWHGEHHSPAVPVPATAEKA"
BASE COUNT         1931 a         2210 c         2421 g         2006 t
ORIGIN      
        1 cagaagatga ttgataactg tctgcaggac attttacgga tttgggataa agataagaac
       61 gatccctggt ttccagttgc cgcccaggtc gagttgtcgg gcgatgacca tatggatggc
      121 aagaatttca tcaacgtgct acaggggctc ggttctttcg aatataaaaa cgttaccgtg
      181 ttatttgcat taatacgctg ctttctgatg acgaatccca cccggctcag gttcatccgt
      241 aaatcgtatc ggggaatttg cgagcccatg tctggatcgt atgcctggat ctggcacaga
      301 attgcctttt cagatgccaa ttttttcgag catctgctcg tatttttggc ttccgatgct
      361 tcgcgccggc atcattatgc tcatatcatt ccaatcctgg aaaatctttt gggctattgc
      421 gtttgtatca gccaggagtg gctcgaaaca cctaataaca gcattccgca cccggctatc
      481 acctgcctgc ctgtggatgc ggagggaaga ccgctgtgtc gactatccga aaaagccatg
      541 caacagaaac gggatctagg attcggtgat tacattccgg atactgacac gacttttctc
      601 actttgggga tggcgagaaa gtggcttgat ttcgtgcaga gggaggaact ggcggtggat
      661 aaagcgctcc tcgaagcttg cgagtcgctg ttggctcatc cctgggtgga gattatcagc
      721 gaataccagg tgggaggtaa atacagttca aatccaccca ccatccagat taccaagccc
      781 ctggattacg agggcgcggt gcctatatgg tttgataaaa cgttcaggaa cgatggcagg
      841 ataattcggg aaatgttagg taatgagatt tgcccaggtc ataacatgga tatcctggat
      901 gccattcttg ttaaccgggc gcaatggtcc gccttggagg gtgaaaacct ggtcttttta
      961 cagcgattgc ttgattttca ttatcaagcc tttgcaagcc gcaatttccg ccacgaaacg
     1021 gccttcaaat attatctgcc agaaatttat gtctattatc ttgggcgaat gtaccgaact
     1081 tatggaacgc tcgctgatat agacaaacgt atccttgacc ctggaggaaa aattgaggat
     1141 atgcgtcgca ttgctctgcg gtactgcaag gatgagctga tggggtattc cctcaatgcc
     1201 ttcgatgccg ccctggcagt atctgcgctt gtgctgctgg cctatgagcc gagacgtgac
     1261 ggagttatag ctgctggcct gaacgtgcta aagcaggcga tgggggaggg gagaggccgg
     1321 cacccttata gagcctatga gtggaaccgc atgcgtcacc ccacccggat tgtggttggc
     1381 agtgaggttg caacttcgct gtttgtgctg agagcctgca cggaagccat gcgctatctg
     1441 gaaaaagagg aatttataaa tcagggaacg tgacagcact gatacgttcg tgccatgggc
     1501 gcggtcggat aatcaaggcc tccattcgtt tatccctacc gttgctgcta tatagtggac
     1561 ccgggggttc catggcgtag tttttaagcc cgcgagcatc gcacatgaag ccgcgtggtg
     1621 cactttcaag cgtatgctcg taatggtcac acagctcgaa aaactcacga gacatcgatc
     1681 acgattcgct ctgtttcatc ctccatcaac gcttcgtgta ccccgcactg gacatccaac
     1741 cttcgggccc cgggtttcgt tggcttttct gttataaatc acaacccttg atcgatatcc
     1801 ggcatgacag cgtcgggctt tgcgtcgatc tttaatcttt aatattggcg cctgcatgat
     1861 atgcggacgg cgaccattcc atcttgatag gcccggtatc ccataaagtt tctccgatcg
     1921 caaactaatt gcttttaatt caatctaacg ggcgcaaaag cgtttcgcat attgcctggt
     1981 ccgatccatg aatttttgtt gcagaccgtt ttgccgctgc ttattgttgg tcggccgggc
     2041 ttgcaggaaa gtctcattct ctttcggact ataattttaa aagagcgaca tgcgatgcca
     2101 ctcgtacgaa agcgcttgca tttgcctggt ttagagacat ttccccaacg aatccggtca
     2161 cgccgaaccg gctaacgaaa gcactcatga acgtatttta cgaagaggaa ggcacattca
     2221 aggtcggcgc tatccttgcg gataatacga catcgctgca ggtcgaggct ccccacggca
     2281 ggcgcgcgaa aataaaggcc gcctctgtac tgctgcggtt tgatacgcct tcgctttccg
     2341 agttcatgga tgttgcagtt aaaacggcgg aggaactgga tccgaatttt ctctgggaat
     2401 gttgcgaaag cgaggcggag ttcgcgagcg aaaccctcgc ctccgattat ttcggtcatg
     2461 cagccaggcc ggaggaagct gcagccgtgt taatccgttt gcacagcgcg cccatatatt
     2521 tctataagcg gggccgcggg cgctataagg ctgctccttc caaagcgctt gaggctgcct
     2581 tggcgagcca ggaaaaaaga cggcggcagg cggaacagca ggcgcactat gcccagttgc
     2641 tgtgcgcatt cagcctgcct gaagaattca agccgatatt gcccagcctc ctctaccgcc
     2701 cggataaaaa cacgattgag tggaaggctc tggaggcggc atgcagcaca acaaaactga
     2761 ccgttcgcaa gctgctggag atgtgcgggg ggattccctc tacacacgat taccatctgg
     2821 accgttttct gctggagcac ttcccggaag ggccaggttt tggggaagtc gatacgaggg
     2881 agttgatgga gctgccggag ctaccggttg cggatgtgac cgctttcagc atagacgacg
     2941 tcacgacgac cgaaatcgat gatgcttttt cggttacacc cttaagtctc ggcagtttca
     3001 gggttgggat acatattgcc attcccacct tgggaatcac gccgggatcg ccgcttgaca
     3061 ggattgcggg gcggcggctt tcgacggtat accttcctgg cagcaagatc acgatgctac
     3121 cggatgccgt gatacagcac tacaccttga gggaaggagg agtctgtccc gccttgtcga
     3181 tgtatcttga cgtggcggat gattttacga taaccggaac aaccagccgg gtcgaccaga
     3241 taaacgttgc cgcgaatcta aggcatgaca cgcttgaaga gtgcttcaac gaatccactc
     3301 tcgcagctgg ctctgtgaat catcatttcg gtaacgaact actggcgtta tggaagtttg
     3361 cgtgcaaact ggaagcaaac aggggcaagg ccaatgacag caacaacgat cggatggatt
     3421 acagtttcag cgttaaggac gaccggatca cgatcagtca gcggcggcgt ggctccccca
     3481 ttgacaaggt tgtatccgaa ctgatgattc atgtgaatgc cgaatggggc aagcagttgg
     3541 ctgacggggg agtcactggc atttatcgca gccagggtgg aggaaaggtt cgcatgagca
     3601 catcccctgc tccgcaccag ggtctgggtg tttcgcagta tgcctggatc agctctccaa
     3661 tgcgccgcta cgtggatttc atcaaccagc ggcagctcgt ggcattgctg cgcggcgaaa
     3721 caccgcccta cgctcgcgag agtgaggatt tgcaaacgta catgcgtgat ttcgagtcgg
     3781 cttacgaggt ttacggggat tttcagcgta gcatggagcg ctactggtgc ctgcgttggc
     3841 tgctgcagga aaaaatcgaa gtaaccagcg cccaggtttt aaaggagaac ctggtgaagc
     3901 tggactcgtt gcctctcgtc acccgcgtat gcggtttgcc ggacattgca ccggaaacca
     3961 gcatacaggt ggagatttca aatattgatc ttctcgagct cggttttacc gcaagattcc
     4021 tgcataagct ccacggctga gcggttgagg cgaatttccc cctcttgatg catattgttt
     4081 gtgacaaagc aattgcctgt cgttagaata cgcatctgcc atcctgtcct gaaatgttca
     4141 gcctgcttgc agtacgttca actgcagtta gttcaacgtt agccacgttg agacgtggat
     4201 gggaagtttt acctcttccc attgcttttc cctccaataa acctcctcgc tgctgcaaga
     4261 attgaatctc gcctcagaca tatccgtttt gcgtcccgct gctggtccgg actggctgct
     4321 gagtccattt cgcctgaacg tggcaatagc ggtttccctg gtcattcacg tggttctgct
     4381 atttggcgtt aatttcaagc ttccgtttac ggggaaagag ctcagccagc cgctggaggt
     4441 ggttctggta aacagcaaat ctgcgaccaa gccgcgcgga gcggatgcgc tggcgcaggc
     4501 aaacctggat ggcggcggta acacggatag caacaggcag aacaaagcgc gaagccggcc
     4561 agccgcgagc cccgacaagg ggattgctgc ggcggagctc agggaaagca cggaaagagt
     4621 ggagcaactc gagcacgaag ccacgcgctt gatgacggcg acgcatggcg ccgcggaggt
     4681 gagccaaccg gttgccgagg tttcgagcca ggccgagcag gcagcgccag ctcattatac
     4741 ggctgatgca cgcgatctcg tgcagcgagg ccttgaaatt gcgcgcctcg aagcgcagac
     4801 ctcacttgac tacgaagctt atcaaaagcg tccgaagcga aaatttatcg gcgcgaggac
     4861 gaaggaatat cgtttcgcgc gttacgtcga ggactggcgt ttcaaggtcg agcgcgttgg
     4921 aaatctcaat tatcctgagg cggcaagacg cgataaactc tacggaagtc tgcagcttac
     4981 cgtgggtatc cgctcggacg gcagcctcga gtcagtcgaa ctcaaccggt cttcaggcaa
     5041 aaaggtactg gatgaggccg caataagaat tgtgaacctg gctggactgg atggctttgc
     5101 accctttccc cccgaaatca gccgggatac cgacatcctt catattaccc gaacctgggt
     5161 attcacacgc tcggacgagc tgatgagcga atagacgatc gacatacagg ttcccagcat
     5221 gcctgatcgt tatgcagtca tcggcaatcc agtcgcccaa agcaaatctc cactgataca
     5281 cgcggaattt tcgctgcaaa cgggtgagga cgtctgctat gaggcaatac tcgcgccctt
     5341 ggacaggttc gcccagacgg tgacggattt ccgggagaag gggggcaaag gcctcaatgt
     5401 caccgttcca ttcaagcttg atgctttcag gatttctgat caggttactg gccgcgctcg
     5461 cgttgcaggg gccgtaaaca cgcttgtatt cgagggacct gatattgtgg gcgataatac
     5521 tgacggggtg gggctggtgc gtgatatcga gtccaacctg ggcttcctcc tgccgggaaa
     5581 gaggatcctg ttaatcggtg cgggcggcgc agcgcgaggc gcgataatgc cgttactcgg
     5641 acgagagccg gctgccctgg tgatcgccaa tcgaacgctt caaaaagctc gggacctgca
     5701 acagaacttt tcgggcgatg ggaacatcct tgccgttacc tatcacgatc ttcagggcga
     5761 gcacttcgat cttgtgatca atgcgacatc aacgagcctg ttcggcgaac tgcccccgtt
     5821 gccagagcac atttttgctc cacaatcttt agcgtacgac atgatgtacg gcaaagggcg
     5881 gacgcctttt ctgcaattcg caaagctgca gggagccgag tacctcgctg acggtatggg
     5941 gatgctggtg gaacaagcag ccgaatcatt cctcgtctgg cgaggagtca ggccaagaac
     6001 cgacgctatc atcgagatgc ttgggaactg cgtttgagct gttttaaaac gcaagatcag
     6061 taaggtgaag cgttttctct ggcgatcgtt ctggcgaatt tccttttttt tgctgattgc
     6121 cggcttgatt taccatctgt ggattttggg ccatgttttt tactggagct actacaaccc
     6181 ctcgagcacg gcattcatgg aggctcggtt ggaggtcctg cggcagaaag acggcgctgc
     6241 caggttgcgg aagcagtgga tttcttacga gcggatttcc ccccatctta aacgcgcgat
     6301 cattgctgct gaggacagca ggttcctgga gcacgaaggt tttgatttcg aggcaatcca
     6361 gaaagcctac gagaaaaact tgagaaaggg caggctggtt gcgggaggtt caaccatcag
     6421 tcagcagttg gcgaaaaacc tgttcttgtc ggacgaaaag acgccctggc gcaaaatcga
     6481 ggaagcgctc atcaccttga tgctcgaaaa catcatgaca aaacggcgca tcatggaaat
     6541 ctatctgaat gtgatcgaat ggggcgaagg cgtgtttggg gccgacgctg caatccgtca
     6601 ttattatggc acttcctctt cctcaatcag tcctgagcaa gccgcacgcc tggctgcaat
     6661 ggttccgaat ccacgttttt acgatcgtca tcgtaatacg ccctggctgt caaaaaaaac
     6721 aggcatcatc ctgagccgca tggggcaggt gcagattcca tagcaaaccc tgcttcatcc
     6781 cagcggctgg cggaaaccgg ggaagtggcg gtattcgcgg tgtctgacag cactttccgt
     6841 tccccattcg tcctgtcata atatcgcgcg attttctccc atctttctgc cgctttcacc
     6901 ggctccgtta gagcagtccc tatgacaaaa gcaaataaac tcggggaagc ggaaaacctg
     6961 caggagcact tgcagcgcgt actctatctg ctgtcccagc gaaagctggc gcaagaactc
     7021 gcggagcata ttcccgagca acagcatgag cgggagctgg agcagaagca gaatcttgcc
     7081 gagttgcagg ctttgctcga caggcttcat cccgccgacg tagctcatat tcttgaagcc
     7141 ctgccgcttc aagaccgtct gacgatctgg gatctggtca aggcggagcg cgacggcgaa
     7201 attcttcttg aagtgtcgga cgccgtacgc gagacgctga tcgccagcat gaacagcgga
     7261 gagatgctcg ctgcggccga gcagctggat gctgacgaga ttgccgatat tgcccccgat
     7321 ctcccacggt atgtcatcga tgacgtgttc cgctccctcc cgatcgagga acgcgaaaaa
     7381 ttgcgggccg ccatgtcgta cccggaggac gcggtgggcg cgctcatgga tttcgatatc
     7441 gtcaccattc gtgaggacgt cacactggaa gtcgtgctcc gttatttgcg cagactcgat
     7501 gagcttcccg atcataccga cctgttgttc gtcgtggatc gtaccgagcg tctcaaaggc
     7561 gtcctgccgt tgaacaggct gctggtcagc gaccctgcgc tcaaggtggg ctccatcatg
     7621 agcaaggaaa tggtcgtgtt tcatccgagc gagaaggccc acgaggcggc tcaggcattc
     7681 gagcgctatg acctggtttc cgcaccggtc gtaaatggtg aagagaagct ggtgggacgc
     7741 gtgacggtga acgccgtcat ggatttcatc cgggaagaat cggcaagcga ggcgctgagc
     7801 ctgggggggc tgaggcagga agaagatttg ttcgcaccca tatggaaaag cctgaagaat
     7861 cgatggacct ggctggctgt caacctgatc accgccttca tcgcctcacg ggtaatcgga
     7921 atattcgaga attcgatcga aaagctggtc gccttggcgg cgctgatgcc gattattgcg
     7981 ggaatcggcg ggaattccgg caatcagacg attacgatga ttgtacgcgc gctggcgttg
     8041 ggccagatca atgctgccaa tgcccgcaac ctcgttatca aggaaattgg agttagcgtt
     8101 gtaaatgggc tcgtatgggg cggagtggtc ggattgttcg ctttcgttat ttatcggagt
     8161 gtgccgcttg gcctggtgat gatgctcgca atgatcttga atctgatcct ggcggcattg
     8221 tccggcgtgc tgataccgct gacgctgtat cgattcggcc gcgacccggc agcgggatcc
     8281 agcgtgatga ttacagccgt gacggatagc ggaggattct ttatcttcct ggggctggct
     8341 acgctttttc tgctgtagcc ggcactggca cggccgggct gtggtgctcg ccatgccaga
     8401 ggggaatcac ttcccccgcc atgagctgtt ccatcgtttc ccgttgccgg atcacgtgga
     8461 acctgtcccc atcgatcatc acctcagcag cccgggcgcg agtattatag ttcgagctca
     8521 tgctcatgcc ataagctccc gccgacatga tcgcgagaag gtctcctt
//
LOCUS       DASSHJ010000180         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_241466, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000180 DASSHJ010000000
VERSION     DASSHJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241466"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFI43_07010"
     CDS             <1..517
                     /locus_tag="VFI43_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7061912.1"
                     /translation="HAGIDGLAKTLSATHIDLLINNAGIYAGSHGTPLEDIDYEAWAK
                     SFHINTMASLKMAQAFLHHIERSKQKKIVTISSKMGSITDNTSGGSYLYRTSKTAVNM
                     VIKTLSIDLKTAGVIAVVFHPGWVKTDMGGPRAQISATQSVSGMRRIIDRLSMADSGK
                     FLSYDGQIVPW"
     gene            complement(652..972)
                     /locus_tag="VFI43_07015"
     CDS             complement(652..972)
                     /locus_tag="VFI43_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET7061913.1"
                     /translation="MQLTTTPNIEGKRITRYCGVIAGEAILGANLFKDLFAGIRDLVG
                     GRSGTYEKELQRAREIALDELEQRARDVGANAVVGIDIDYEIMGKENGMLMVSASGTA
                     VVVE"
     gene            complement(1072..1341)
                     /locus_tag="VFI43_07020"
     CDS             complement(1072..1341)
                     /locus_tag="VFI43_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061914.1"
                     /translation="MEKSHTMPSHHRLKELLAIPERKRTDAEWAEINELEITLTPINR
                     AAAHEQTPRRREASSPAPPKSRDTAHLKQALKKPRKRPPRGGSGA"
     gene            1426..2433
                     /locus_tag="VFI43_07025"
     CDS             1426..2433
                     /locus_tag="VFI43_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7061915.1"
                     /translation="MQPRFYHYLSVLLYCSSVFGGSAHADEVVVYSARIEQLIKPMFD
                     AFTKDTGIGVKFVTDKEGPLLARLKAEGKNTPADVLITVDAGNLWEAAREGLLRPVES
                     KVLEANIPAYLRDPNNEWFGLSVRARTIIYNTQKVKPSDLSTYEALADPKWKGRLCLR
                     TSKKVYNQSLVATMIAEYGEPKTEQIVRGWVANLAMDPLSDETKAMEYVAAGRCDVTL
                     ANTYYYGRLMEKKPSLPLAIFWPNQEGSGVHVNISGGGVTRYADNVQGGIKLLEFLSS
                     EKAQNLFADVNMEYPVNPRVKTDPVVASWGTFKQNPINVKKAGELQAEAVKLMDRAGY
                     R"
     gene            2441..4132
                     /locus_tag="VFI43_07030"
     CDS             2441..4132
                     /locus_tag="VFI43_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7061916.1"
                     /translation="MESEAIDPAESNVTSRQGSSHFSTSVYRDSPRWRLTPILVAALV
                     LVPLGTVISSFFAPATDIWQHLVETTLPGLLTNTFWLVLGVALGVVLLGVSLAWLTAV
                     CEFPGRRFLSWGLLLPLAVPAYVTAFVALGLFDYVGPVQTALRAWLGSNRFWFPDIRS
                     RGGVVAVMVLAFYPYVYLLARNAFLTQGKRSLEAAQSLGLGRTQGFFKVALPMARPWI
                     AAGLMLALMETLADFGTVAVFNYDTFTTAIYKAWFGMFSLSAASQLASLLIVIVFAVI
                     IIEQQFRSRTRYAESRPGARVDRVHLIGRRAWLATGFSWSVFFLAFLLPVMQLFLWAS
                     DVFARDFDQRYLEFLWHSLLLSAAAAALTCSVALLLVYAARRHPDPLTYASVRVATIG
                     YALPGAVLAVGIFIPVAWLDNQLSDLALQLFHIETGLIIQGTLAIMLIAYLVRFLAVG
                     HGPLDSAMQRVTSSLDEAAMGMGLDGWAILRRVHLPMLKGGLFTAATMVFVDVMKEMP
                     ITLMTRPFGWDTLAVRVFELTSEGQWEHAALPSITLVLAGLIPIAFFMRQSDRSG"
     gene            4137..>4942
                     /locus_tag="VFI43_07035"
     CDS             4137..>4942
                     /locus_tag="VFI43_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7061917.1"
                     /translation="MHALLDLRNIHHAYGPHVVVKNLSLALKKGQIGCLLGPSGCGKT
                     TVLRCIAGFERITGGEIMLKGAAVSRANLYLPPEQRHIGMVFQDYALFPHLTVAGNIG
                     FGLQRLSRNEREARISEMLDTVGMVEASRKYPHELSGGQQQRVALARALAPRPDLLLL
                     DEPFSNLDVSLRERLSLEVRDILKSQNATAILVTHDQDEAFAMADEIGVMHDGEIQQW
                     DTAFNLYHQPANRFVADFIGQGVFLPGKMLDPHNVEIELGVLSGTVAHECT"
BASE COUNT         1038 a         1407 c         1337 g         1160 t
ORIGIN      
        1 ccatgcagga attgacgggc tcgcgaaaac gctgtcggca acacacatcg acttgctcat
       61 caataatgct gggatttacg cgggatccca cggcacgccg ctggaggata tcgattacga
      121 agcatgggcc aagtcattcc atatcaatac tatggcaagc cttaaaatgg cccaggcttt
      181 tctgcatcac attgagcgca gcaagcagaa aaaaatcgtg acgatcagca gcaagatggg
      241 cagcatcacg gacaatacca gcggaggcag ttacttgtac cgaaccagca agactgcggt
      301 aaacatggtt atcaagacgc tctcgattga cctcaagacc gccggagtca tcgcagtcgt
      361 tttccatccc gggtgggtaa agaccgatat gggcggcccg agggcgcaga tttcggctac
      421 ccaaagtgtt tccggaatgc gccggatcat tgaccgcctt tccatggccg attccggcaa
      481 gtttctgtcg tatgacggac agattgtacc gtggtaattt ctattaaaac atttgctttc
      541 tgaccctctc ccccgacccc tctcccataa gtgggagagg ggggtgagga gttgcttcgc
      601 gctaagggag tagcattgcc ttaaggcagc gagggtgacc ggaaatgcga tctattcaac
      661 gactaccgcg gttccgctcg cggaaaccat cagcattcca ttttccttgc ccatgatttc
      721 gtaatcaata tcgattccta ccaccgcgtt tgccccaaca tccctcgcac gttgctcgag
      781 ttcgtcgaga gcaatctcac gggcgcgttg cagttccttc tcgtaagttc cagaacggcc
      841 tcccacgagg tcacggatac ctgcaaacag atctttgaaa agattggcgc caagaatggc
      901 ctcaccggcg atgacgccac aatatcgggt gatgcgtttc ccttctatgt tcggtgtcgt
      961 cgtaagttgc aattgtctca tcctctttaa aattcgggct tctgttgatc ataataccgg
     1021 caaacagttt tatccccgca gcgggaaacc gccgtattct aagcttcttc gctatgcacc
     1081 gcttcctccc ctcggcggtc ttttccgcgg cttcttcagg gcttgcttga ggtgcgccgt
     1141 atccctggac ttcggcggag cagggcttga agcctccctg cgccttggcg tctgttcgtg
     1201 cgcagcagcc cgattgattg gagtgagcgt aatctcgagt tcattgatct ccgcccattc
     1261 cgcgtccgtc cgcttcctct ccggaatcgc cagaagctct tttaaccgat ggtgagacgg
     1321 catcgtatgc gatttctcca tgacagccgg taactcagta ccgagtttta ctgataatta
     1381 ttatcattta taatagggaa aatttcgtga ttaccaggag aacaaatgca gccccgcttt
     1441 taccattatc ttagcgtact cctttactgt tccagtgtct tcggcgggtc agcgcatgcg
     1501 gacgaagtgg ttgtctattc ggcccggatc gagcagctca tcaaacccat gtttgacgcc
     1561 tttaccaagg ataccggcat cggcgtcaag tttgtgacgg acaaggaagg gccgttgctg
     1621 gcgcggctca aagcggaggg aaaaaatacg cccgcggacg tattgatcac cgtagacgcc
     1681 gggaacctct gggaagctgc cagagaggga ttgttacgtc ccgtcgagtc caaagttctg
     1741 gaggccaaca ttcctgctta tctccgcgat ccgaacaacg aatggttcgg gctttcggtt
     1801 cgtgcccgaa ccatcatcta caatacgcag aaagtgaaac catccgatct ttccacttat
     1861 gaagcactag cggacccaaa atggaaggga aggctatgtc tgcgaacttc caagaaagta
     1921 tacaaccagt ctctggtagc caccatgatt gccgaatacg gtgagccgaa aaccgagcaa
     1981 atcgtgaggg gctgggtggc caacctagcc atggatcctc tctccgacga aaccaaggca
     2041 atggaatacg tcgcagctgg gcggtgcgac gtaaccctgg caaatactta ctactatggg
     2101 cgcctgatgg agaaaaaacc ctctctgcca ctggcaattt tctggcccaa ccaggaggga
     2161 agcggcgttc atgtaaatat ctccggggga ggcgtaactc ggtacgcgga taacgtgcag
     2221 gggggaatca agctgctcga atttctttcc tcggaaaaag cgcaaaacct gtttgccgac
     2281 gtgaatatgg aataccctgt caatcctcgt gtcaagactg atccggtcgt cgcaagctgg
     2341 ggcacgttca agcaaaatcc aataaatgtc aagaaggctg gcgagttgca agccgaagcg
     2401 gtgaaactga tggatcgcgc cggctatcga taggcacaag gtggaaagtg aggcaatcga
     2461 tccggcggaa agcaacgtaa cctcgaggca aggttcttcc catttctcaa cttccgtata
     2521 ccgcgactcg ccgcgttggc gcctgacccc catcctcgtc gccgcgcttg tccttgttcc
     2581 cctcggaacc gtcatttctt cattctttgc ccctgccacc gatatctggc agcatctcgt
     2641 cgaaacgacg ctgcccggcc tgctcaccaa tacattctgg cttgtgctgg gtgtcgccct
     2701 cggggtggtg ctcctgggcg tcagcctcgc ctggctgact gccgtttgtg aatttcctgg
     2761 aagaaggttt ctttcgtggg gtctattgct gccccttgcc gttcccgcat acgtgaccgc
     2821 gttcgtggcg ctcgggcttt tcgattacgt ggggcccgtg cagaccgcgc taagagcgtg
     2881 gctgggatca aaccgcttct ggtttccgga tattcgaagc agaggcgggg ttgttgctgt
     2941 catggtcctg gcattttacc cctacgtcta cctgctggcg cgcaacgcct ttctaaccca
     3001 gggcaagcgt tcgctcgagg ctgcgcaatc gctgggcttg ggccgcaccc aaggtttttt
     3061 caaggttgcc ctcccgatgg cgcgaccgtg gattgccgcg gggctgatgc ttgccctgat
     3121 ggaaacgctt gcggatttcg gtacagtagc cgtattcaac tatgacacat tcacgacggc
     3181 catctacaag gcctggttcg ggatgttttc attatccgct gcttctcagc ttgcctccct
     3241 gctgatcgtg atcgtctttg cggtgatcat catcgagcag cagttccgtt cccggacccg
     3301 ttatgcagaa agcaggcctg gcgcgcgagt ggacagggtt catctcatcg gacgaagggc
     3361 gtggctcgcc acaggctttt cctggtcagt tttttttctg gcattcctgc taccagtcat
     3421 gcaattgttc ctctgggctt ctgacgtgtt tgcacgcgat ttcgaccagc gctacctcga
     3481 attcctctgg cactcactgc tgctgtcagc agccgccgcc gcgctgactt gttccgtcgc
     3541 cctgcttctc gtctacgcag cacgccgcca tcccgatccg ttgacttatg cctcagtgcg
     3601 ggtggccact attggctatg cgctgcccgg cgctgtcctg gccgtcggca tcttcattcc
     3661 cgtagcttgg ctggacaacc agttgagcga cctcgcactg caactgttcc atatcgaaac
     3721 cggactgatc atccagggga cacttgccat catgctcatc gcctaccttg tccgcttcct
     3781 ggccgtgggc cacggccctc tcgacagtgc catgcaacgc gttacgagca gcctggatga
     3841 ggccgcgatg ggaatgggcc tggacggatg ggcgatttta cgccgggttc acctgccgat
     3901 gcttaaaggc ggcctgttta ccgccgccac aatggtattc gtcgacgtca tgaaagaaat
     3961 gcccatcacc ttgatgaccc ggccattcgg ctgggatacc ctggcagtga gggttttcga
     4021 gctgacctcg gaagggcagt gggagcatgc tgcccttccc tcgattacgc ttgtgctggc
     4081 agggctcata cccattgcgt tcttcatgcg gcagtcagac cgttccggat agtcgcatgc
     4141 acgcgttgct cgacctcagg aatattcacc acgcctatgg tccgcacgtc gtcgtcaaga
     4201 acctgtccct ggcactcaaa aaaggccaga tcggctgtct tctgggtcct agcggctgtg
     4261 gcaaaacaac agtgctgcgc tgtattgccg ggttcgagcg catcaccggc ggcgagatca
     4321 tgctaaaagg ggctgcggta agcagggcga atttatattt gccgccggaa caacggcata
     4381 tcggaatggt attccaggat tacgcgctgt tccctcacct taccgtcgcg ggaaatatcg
     4441 gctttggtct gcaacgtcta tccagaaacg aacgcgaggc gaggatctcc gagatgctcg
     4501 acacggtagg catggtcgag gcatcaagaa agtatcccca tgaactttcc ggaggtcagc
     4561 agcagcgtgt ggcgctggca cgcgcactcg cccctcgtcc cgacctgcta ttgctcgacg
     4621 aaccattctc caatctggat gtaagcctca gggaacggct gagcctggaa gtgcgcgata
     4681 tcctcaaaag ccagaacgcg accgcgatcc tggtcaccca tgatcaggat gaagcgtttg
     4741 cgatggcgga tgagatcggc gtgatgcatg acggcgaaat ccagcagtgg gatacggcat
     4801 tcaaccttta tcaccagccc gctaaccgct ttgtcgcgga ctttataggg caaggcgtat
     4861 ttctccccgg gaaaatgctc gatccacaca atgtggagat agagttgggc gttctgtcag
     4921 gaacggttgc ccatgaatgc ac
//
LOCUS       DASSHJ010000181         9015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1264601,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000181 DASSHJ010000000
VERSION     DASSHJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264601"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2707
                     /gene="secA"
                     /locus_tag="VFI43_07040"
     CDS             <1..2707
                     /gene="secA"
                     /locus_tag="VFI43_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7061918.1"
                     /translation="SRNDRLIKQYSQNVRGINALEEPIAALTDTELRAKTGEFRQRLG
                     EGAVLDTLLPEAFAVVREASKRVLGMRHFDVQLIGGMALHEGKIAEMRTGEGKTLMAT
                     LPAYLNALPGKGVHLVTVNDYLAKRDAEWMGRIYRFLDISVGVILSQMDHGDKQSAYA
                     ADITYGTNNEYGFDYLRDNMVGHPAERVQRLLNFAIVDEVDSILIDEARTPLIISGQA
                     EGNTDVYVRINALIPKLVRQESESASGDFSVDEKAQQVLLSEAGFEHAEKLLVQSGLL
                     PAGTSLYDPANISLVHHLYAGLRAHALFHRDQHYVIQNGEVIIVDEFTGRLMAGRRWS
                     EGLHQAVEAKEGVSIQKENQTLASITFQNYFRMYQKLAGMTGTADTEAYEFQQIYGLE
                     TVIIPTHRPMVRIDRMDQVFRTMDEKHKAIIEDIKDCHQRGQPVLVGTTSIENNELLS
                     SLLTRDKLPHQVLNAKQHAREAEIVAQAGQPKMITIATNMAGRGTDIVLGGNPEPELE
                     RLRSDENLSEAEKQERSAEVDQQWHVLHDEVLKQGGLHIIGTERHESRRVDNQLRGRA
                     GRQGDPGSSRFYLSLEDPLLRIFASDRVANIMQRLKMPEGEAIEHPWVTRAIENAQRK
                     VEARNFDIRKQLLEYDDVANDQRRVIYQQRNELLESQDISETVTAMREDMLGNLIAVH
                     VPPNSVEDEWDIPGLEKALASEYQVSLPLIEWLEKEPDLHEETLQERISEAARAHYQA
                     KVDLVGTSVMHQYERAVMLQSLDAHWREHLAALDHLRQGIHLRGYAQKNPKQEYKREA
                     FELFTSMLEEIKSEVTRTLLTVQIRSEQQVEAVAEAQPQPVNVQYKHAAYEDALGEEQ
                     AQEVAETQERSEKQQPFVRGQKIGRNDPCPCGSGKKYKQCHGKLS"
     gene            complement(2809..3363)
                     /locus_tag="VFI43_07045"
     CDS             complement(2809..3363)
                     /locus_tag="VFI43_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HET7061919.1"
                     /translation="MRTFAGVVTTILAISLAGCTNVPKPVVQNEKMSDGDLTVPANYK
                     SWPKFLSDIQRADSKQVRDIYINPIGNSTKMGDAFPNGTITVMEIYKAREAADGTPLK
                     GPDGKFVKGNLVKIGVMGKGPGWGDTVSPPELRNGDWVYSMYMADGKTKAPDDTATCR
                     ACHLPLKDKDYIFRYDEYFQKRNG"
     gene            complement(3617..5716)
                     /locus_tag="VFI43_07050"
     CDS             complement(3617..5716)
                     /locus_tag="VFI43_07050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7061920.1"
                     /translation="MNPLLDFSGLPRFSDIRPEHVTPAVDQLLSENRALLERIRNESS
                     APAWENFVQPLEDANERLSRAWGPVSHLNAVVNTPELRNVYNSNLPQVTQYYAELGQD
                     QVLFEKFKALKDNEQYEKLSPARKKIVENALRDFRLGGAHLPPAEKERFLKIQEELSA
                     LSSTFNDNLLDATNNFVLHVQNREEVAGLPDDVLQMARSAAEQEGVSGWKFSLHAPSY
                     LPVLQYAENRALREKMYRAYSTRASEIEARGVLADTAGKYDNGPLIRRILELREEEAR
                     ILGFDCYAEVSLATKMASSPQQVLDFLGELGTKARPYAEHDLEELREFASSQLGLQDL
                     EAWDIAYASEKLRVDRYAFSDQEVKQYLPETSVLPGMFRLVQHLYGITIDQVTEGVQL
                     WHPDVKFFSILDKQGKLIGHFYLDLYARAGKRGGGWMDDAVSRRQLRSSGSGVPGIQA
                     PVAYLVCNFSAPVQMNGQMRPALFTHDEVITLFHEFGHGLHHLLTQVEDLGVSGIHGV
                     EWDAVELPSQFMENFCWEWEVMRNMTKHVDTGEPIPRALFDKMLAAKNFQAGMQTVRQ
                     IEFALFDMHLHSDFDPHGKKTVQQLLNEIRRQVAVIIPPEFNRFPNSFSHIFGGGYAA
                     GYYSYKWAEVLSADAYSLFEESSAGTVVNPETGALFWNEILAVGGSRSALESFIAFRG
                     REPKIDALLRHNGMAAA"
     gene            complement(5870..7156)
                     /locus_tag="VFI43_07055"
     CDS             complement(5870..7156)
                     /locus_tag="VFI43_07055"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7061921.1"
                     /translation="MEIVIEQGRVVDPKNSLDAIMDIYISAGRIAALGTPPAGFRPER
                     VIDARDTVVCPGLIDLAARLREPGFEYKATLESEMAAAVAGGVTSLACSPDTDPVLDE
                     PGLVEMLKHRAKSLNKANVYPIGALTRGLKGDWLTEMAELRDAGCVAFGQADKPLPNP
                     RVLMQAMQYAATFGFILWLRPQDAYIAGEGVAHDGEVATRLGLPAIPVSAETLALSNI
                     ILMARETGARVHLCRISSAEGVEMIRAAKRQGLPISADVTINHLHLSEMDIGFFDSNC
                     HLVPPLRGLSDRDALRRSLMDGTIDAICSDHAPVDEDAKLLPFGEAEAGATGLELLLP
                     LTLKWASEAKVALSAALARITVEPGRILGPDIGHLTPGAVADVCVFDPLQDWRVEPAA
                     LKSQGKNTPFLGMELQGRVIYTLVSGNIVHEASGIR"
     gene            complement(7158..8108)
                     /locus_tag="VFI43_07060"
     CDS             complement(7158..8108)
                     /locus_tag="VFI43_07060"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7061922.1"
                     /translation="MSSNPQLNKNGELQHLLSTEGLPPSVLLKILDTAESFVGVTERD
                     VKKVPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSADVVNLNIAASSQSKGETLLDTV
                     NNLSAMHADMFIVRHSQSGAAHLIARHVRADIHVINAGDGRHAHPTQGLLDAFTIRRY
                     KRDFHNLRVAIVGDILHSRVARSQIHALTTLGVPEVRVIAPKTLLPSKVERLGVHVYH
                     KMTEGLKDVDVLLMLRLQNERMSSAMLPSKEEYFKYYGLTQEKLALAKPDAIVLHPGP
                     MNRGVEIDSAVADGKQSVILPQVTFGIAVRMAVMSILAGN"
     gene            complement(8101..8619)
                     /gene="pyrR"
                     /locus_tag="VFI43_07065"
     CDS             complement(8101..8619)
                     /gene="pyrR"
                     /locus_tag="VFI43_07065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET7061923.1"
                     /translation="MELPDAEQLLASLADQLRPAVEKNAALALVGIHTGGAWLAERLH
                     RKLGLSLPFGTLDISFYRDDFDRIGLHTQVKPSDIPFDVDGASIILVDDVLYTGRTIR
                     AAMNELFDYGRPAAVQLAALVDRGGRELPIAPEYVGTVLQLPLGQMLALKRSFSGELS
                     LHLYDKTLRSDE"
     gene            complement(8606..>9015)
                     /gene="ruvX"
                     /locus_tag="VFI43_07070"
     CDS             complement(8606..>9015)
                     /gene="ruvX"
                     /locus_tag="VFI43_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7061924.1"
                     /translation="GFDFGAKRIGVSVGNLGLCLAHPLVTITGSNGEQQFDRIFELIR
                     QWRPILLVVGLPTHTDGKEHELTRRSRRFARRLEGRFGIKTVLVDECYTSIDASTALR
                     EAGIKGKKQKPVLDQVAAQLILQSYFDRYNGTA"
BASE COUNT         2130 a         2415 c         2465 g         2005 t
ORIGIN      
        1 cagccgaaac gatcggttga tcaagcagta ttcccagaac gtaaggggca tcaacgctct
       61 cgaggaacca atagcagcgc ttactgatac cgagctccgt gccaagaccg gggagttcag
      121 gcagaggctg ggtgaaggag ccgttctcga tactcttttg ccggaggcgt tcgcggtcgt
      181 gcgggaagcg agcaaacgcg ttttggggat gcggcatttc gatgtgcaac tgatcggcgg
      241 catggcgctg cacgagggta agattgccga aatgcgcacc ggtgagggca agaccttgat
      301 ggcgaccctg cctgcctatc tcaatgcgct gcccggcaag ggagtgcact tggttaccgt
      361 aaacgattat ctggcgaagc gcgatgctga atggatgggc cggatttacc ggtttctcga
      421 catcagcgta ggcgtcatcc tttcccagat ggaccacggg gacaagcaga gcgcatacgc
      481 cgcagacatc acatacggga ccaacaacga atacggcttt gattacctgc gcgacaacat
      541 ggtgggccat cccgctgagc gggtacagag gctgctcaac tttgcgatcg tcgacgaggt
      601 ggactcgatt ctgattgacg aagcgcgcac gccgctgatc atttcaggtc aggcggaggg
      661 taatacggat gtgtatgtgc gtattaatgc cctgattccc aagctcgtgc ggcaggagag
      721 cgagagcgcg tcgggggatt tcagcgtgga cgaaaaggct cagcaggtat tgctgagcga
      781 agcggggttc gagcacgcgg aaaaactgct tgtccagagc ggattgctcc cggcgggtac
      841 gagcctatac gacccggcga atatcagcct ggttcatcat ctctatgcag gcttacgggc
      901 ccacgcattg ttccaccggg accagcacta cgtgatacag aatggtgaag tcatcatcgt
      961 agatgaattc accggccgtc tcatggcagg aaggcgttgg tcggaagggc tgcatcaggc
     1021 ggtcgaagca aaagaaggcg tttccataca gaaagagaat cagacgcttg cttcgattac
     1081 tttccagaac tatttccgga tgtaccagaa gctcgcgggg atgacgggaa ccgcggatac
     1141 cgaggcttac gagtttcagc agatttatgg actcgagacc gtgattatcc cgactcatcg
     1201 tccgatggtc cgcatagaca gaatggatca ggtttttcgt acgatggatg agaaacacaa
     1261 agcaatcatc gaggacataa aggattgcca tcagagagga cagcctgttc tcgtaggtac
     1321 aacttccatt gaaaacaacg aattgctgtc cagcttgctc acccgcgaca aattgcccca
     1381 ccaggtgttg aacgccaagc agcatgcgcg tgaagcagag atcgttgctc aggcgggcca
     1441 gcccaagatg attacgattg cgacaaatat ggctggacgg ggaacggata ttgtactggg
     1501 aggcaatccg gaaccggagc ttgagcgtct gcgctcggat gaaaatttaa gcgaagcgga
     1561 gaaacaggaa cgaagcgccg aagtggatca gcaatggcat gttcttcacg atgaagtgct
     1621 gaagcagggg gggttgcata tcatcgggac agagcggcat gagtcccggc gagtggataa
     1681 ccagcttcgc gggcgcgcag gccgccaggg cgatccgggc tccagccgct tttatctttc
     1741 gctggaagat cctttgctga gaatatttgc gtccgatcgc gtggcaaata tcatgcagcg
     1801 tcttaaaatg cccgagggag aagcgatcga gcacccctgg gtgacgcggg cgatcgaaaa
     1861 cgcgcagcgg aaggtggagg ctcgcaattt cgatatccgc aagcaattgc ttgaatatga
     1921 cgatgtcgcc aacgatcaga ggagagtcat ttatcagcag cgtaacgaac tccttgagtc
     1981 gcaggacatc tcggaaacag tgactgcgat gcgtgaggat atgctcggca atcttattgc
     2041 cgtgcatgtc cctcccaaca gcgtggagga cgagtgggat atacctggcc tcgaaaaggc
     2101 attggcttcg gaataccagg tttccctgcc tttaatcgag tggctggaga aagagcctga
     2161 cctccacgaa gagacccttc aggagcggat ttcggaggct gcccgagccc attatcaggc
     2221 gaaagtcgat ctggtcggca cgagcgtcat gcatcagtac gaacgtgccg ttatgttgca
     2281 aagcctggac gcacattggc gggagcatct tgctgcactg gatcacctac gccaaggcat
     2341 ccatctccgg ggatatgcac aaaagaatcc caagcaggaa tacaagcgtg aagcgtttga
     2401 attgtttacc tcaatgctgg aggagataaa gtccgaggtg actcgaacat tgctgacggt
     2461 acagataaga agcgaacagc aggtcgaagc cgtggcggag gcacagccgc aacctgtgaa
     2521 cgtccaatat aaacatgcgg cgtacgagga cgcgcttggc gaagagcagg ctcaggaagt
     2581 ggccgagact caggagcgca gtgaaaaaca gcaaccgttt gtgcggggtc agaaaatcgg
     2641 ccggaatgat ccttgcccgt gtggatcagg aaagaaatac aagcagtgcc atggcaaatt
     2701 aagctaaagc ttgaattacc tccccaacga caaatttatt gctccccgga ataagctgcc
     2761 ggcataagtg ccggcagctc gcgatgcaga aatcccggat tctccctatc agccattgcg
     2821 tttctgaaaa tactcgtcat agcggaagat ataatccttg tccttcaagg gcagatgaca
     2881 ggcacggcag gttgcggtgt catcaggagc cttcgttttt ccgtccgcca tatacatgga
     2941 gtaaacccaa tccccgtttc gaagttcagg tggactgact gtatcgcccc agccagggcc
     3001 ctttcccatc acgcctattt ttaccaagtt acctttgacg aactttccgt ccggtccctt
     3061 caaaggagtg ccatccgcag cctcgcgcgc cttgtaaatt tccatcacgg taatggttcc
     3121 attcggaaag gcatccccca tctttgtgct gtttccaata gggttgatat aaatatcacg
     3181 cacctgcttg ctgtccgcac gctgtatatc agatagaaac ttgggccagc tcttgtaatt
     3241 tgccggcact gtcagatcgc catcactcat tttttcattt tgaactaccg gcttcggtac
     3301 gttggtgcaa ccggccagtg aaatagccaa tatcgtggta acaactcctg cgaacgttct
     3361 catccttttt tctcctttta tctgccgagc tggatatgcc tcagcctgtg cctttgtccg
     3421 aaatatgcat gagcgcacgg cgggtacttc accgatgcgg ttttcactgt tgccggagca
     3481 atctaacaca gcagtggcga gatgacaaag ggcaagattg gaaatggcag gcttgaaggc
     3541 atgagatgga gaacggcgcg cgaaacgggc atacaagggg aaacattcgc ggattcacgg
     3601 cttgttcgga ggttccctac gctgccgcca tgccgttatg gcgcagcaag gcgtctattt
     3661 tcggctcgcg ccctcggaaa gcaatgaacg actccagcgc ggagcggctt cctcctaccg
     3721 caagaatctc attccagaaa agggcgcccg tttcaggatt aacgacagta ccggccgaac
     3781 tttcttcgaa aaggctgtag gcgtcggccg acagaacttc agcccatttg taactatagt
     3841 aacccgccgc ataaccaccg ccgaagatgt gggagaagct attgggaaag cggttgaatt
     3901 cgggtggaat gatgaccgct acctggcgcc ggatctcatt caggagttgc tgtacggttt
     3961 ttttgccatg cgggtcgaaa tcgctatgca aatgcatatc gaacagcgca aattcgatct
     4021 gccggactgt ctgcatgccc gcctgaaagt tcttggcggc aagcatcttg tcgaatagcg
     4081 cgcgtgggat gggctccccc gtatccacgt gcttcgtcat attacgcatt acttcccatt
     4141 cccagcagaa gttttccatg aattggctcg gcagttccac tgcatcccat tccacgccat
     4201 gaatgcccga caccccaagg tcttccactt gggtcagcaa gtggtgcaac ccgtgaccga
     4261 attcgtggaa caaggttatc acctcatcat gagtgaacag cgcggggcgc atctgtccat
     4321 tcatctgcac aggggcggag aaattacaaa cgagataggc aacgggagcc tggatgccag
     4381 gaacacctga gccggaactc ctcagctggc gtctgcttac cgcatcgtcc atccaaccgc
     4441 cgccccgctt gccagcccgt gcatagaggt cgaggtaaaa gtgcccgatc agttttcctt
     4501 gcttgtcgag gatactgaaa aatttgacat cgggatgcca gagctggaca ccctcggtga
     4561 cctgatcaat ggtgatgcca taaaggtgct gcaccagcct gaacatgccg ggcagcacac
     4621 tggtttccgg aagatactgc tttacctcct ggtctgagaa tgcataacga tccacgcgca
     4681 gtttctcact ggcataggca atatcccacg cttccaggtc ttgcagaccc aattggctac
     4741 tcgcaaattc acgcagttct tcaaggtcgt gttcggcata aggcctcgct ttcgttccga
     4801 gctcaccgag aaaatccagg acctgttgag gagatgaggc catttttgtc gcaagcgata
     4861 cttccgcgta acaatcaaaa ccgagtattc gagcttcttc ctcacgcagc tcaagtatcc
     4921 gccggataag agggccattg tcatatttcc ccgcggtgtc cgcaagtacg ccccgcgcct
     4981 cgatctcgct tgcccgggtt gaataagctc gatacatttt ttctcgtagc gcacggttct
     5041 ctgcatattg caggacgggc aggtaggaag gcgcgtgcag cgaaaatttc caaccggaga
     5101 caccctcttg ctccgccgcg gagcgcgcca tctgcaacac atcatcggga agtcctgcta
     5161 cctcttcccg gttctgcaca tgcaggacaa aattgttcgt tgcgtcgagg aggttgtcgt
     5221 tgaatgtcga agaaagggct gataattcct cttggatctt aagaaaacgc tccttttccg
     5281 ccggaggaag atgggctccg ccgagacgaa aatcacgcaa tgcgttctca actatttttt
     5341 ttcgtgccgg gctcagcttt tcatattgct cattatcctt gagcgctttg aatttctcaa
     5401 aaagaacctg gtcctggcct agctctgcat aatattgggt gacctgcggc aggttggagt
     5461 tatagacgtt gcgcagttcc ggcgtgttca cgaccgcatt caggtgggat accggccccc
     5521 aagcccgcga aagccgctcg tttgcatctt ctagaggctg cacaaaattt tcccaggcag
     5581 gtgccgaaga ttcattccgt atcctctcaa gcagggctcg attctctgaa aggagctggt
     5641 cgactgcagg ggtaacatgc tcgggccgaa tatccgagaa tcgcgggagg ccggaaaaat
     5701 cgagtaatgg gttcatgctc ttatctgaat attaacgtga aggatttagc cgttgtttga
     5761 tggaaacagc gtatccctgt tcattcttga tacagggaca gcgaaaacat gtatttccag
     5821 aagagtacta tattcgagga gctttgagga tcttcggaca gtggttgctt cagcggattc
     5881 ctgaagcttc gtggacaata ttgccgctga ccaaggtata tattaccctg ccctgaagtt
     5941 ccatgcctaa aaaaggcgtg ttcttgccct ggcttttaag cgccgcaggc tcaacccgcc
     6001 agtcttgcag ggggtcaaac acgcagacgt ctgctacggc tcctggggtg aggtgaccga
     6061 tatccgggcc aagtatacgg ccgggctcga cggtgattct cgccaaggca gcagacagcg
     6121 ctaccttggc ttcactggcc catttcaagg taagcggcag cagaagttcg agtcccgttg
     6181 cacccgcctc agcttcccca aagggaagca gcttggcgtc ctcgtccact ggagcgtgat
     6241 cggaacagat cgcatcgatc gttccgtcca tcaagctgcg tcttagcgca tcacggtcgc
     6301 tcagcccgcg aagcggcggc acaagatggc aattggaatc aaaaaaacca atatccattt
     6361 cagatagatg aaggtgatta atggttacat cagcgctgat gggcaggcct tgccgcttcg
     6421 cggcccgaat catctcaacg ccctctgcac ttgagatacg gcacaaatgc actctcgccc
     6481 cggtttcccg cgccatcaga atgatatttg ataacgcgag cgtctccgca gacaccggga
     6541 ttgcaggcaa tcccaagcgc gttgccactt ctccatcatg tgccacgcct tctcccgcaa
     6601 tataagcatc ctgcggccgc aaccacagga taaagccaaa tgttgccgca tattgcattg
     6661 cctgcatcag cacacgaggg tttgggagcg gcttgtcggc ttgtccgaat gcaacacaac
     6721 ccgcatcgcg caactcggcc atctcggtca gccaatcacc cttcaagcct cgggtgaggg
     6781 caccaatagg gtagacattc gccttgttca ggctcttggc gcgatgcttc agcatctcga
     6841 ccagcccggg ttcatccagg accgggtccg tatcgggaga gcatgcgaga ctcgtgactc
     6901 cgcccgctac cgctgcggcc atctcggatt ccagcgttgc tttatattcg aatccgggtt
     6961 cacgcaagcg cgctgcaaga tctatcaagc cagggcatac caccgtatct cttgcatcaa
     7021 ttacgcgttc agggcgaaac cctgcgggtg gagtgccaag cgcggcgatt cgtcctgcag
     7081 atatatagat atccattatg gcatcaagcg aatttttggg atcgacaacg cgaccttgct
     7141 cgatcacaat ttccacttca atttcctgcc aggatggaca tgacggccat tcgtacagcg
     7201 ataccgaacg ttacttgcgg aagaatgacg gactgcttgc cgtccgctac ggctgaatca
     7261 atttcgacgc cgcggttcat cgggcccggg tgcaatacga tcgcgtcggg cttggccaag
     7321 gcaagctttt cctgagtcaa gccataatat ttgaagtact cttccttgct cgggagcatt
     7381 gcgctcgaca tgcgttcatt ttgcaaacgc aacatcagca gcacgtcaac atccttcagc
     7441 ccctccgtca tcttgtggta aacgtgcact ccaagccgtt caacctttga gggcagcaag
     7501 gttttaggtg caataacccg aacttccggt acgcccaacg tggtaagcgc atggatctgg
     7561 gagcgcgcga cacgtgagtg cagaatatct ccgacaatcg ctacgcggag attgtgaaaa
     7621 tcacgcttgt aacgccgtat ggtaaatgcg tcgagcaagc cctgcgtggg atgagcgtgg
     7681 cggccgtctc ctgcattgat aacgtggatg tccgcacgta catggcgagc aatcaggtgg
     7741 gcggcgccac tttgggaatg ccgcacgata aacatatcgg catgcatggc agagaggttg
     7801 ttgactgtgt ccaggagcgt ctcgcctttc gactgggaag aagcggcaat gttaagattg
     7861 acaacatccg ccgataaacg cttcgcggca atttcaaagg tggttcgggt gcgtgtgctc
     7921 ggctcaaaaa aaaggttgaa tactgacttt ccccgcaaaa gagggacttt tttaacatcg
     7981 cgctcggtaa ccccgacgaa ggattcggca gtgtcgagga tcttcagcag aacagagggg
     8041 ggaagtccct ccgtactgag caggtgttga agctccccat ttttgtttag ttgaggattg
     8101 ctactcatcg ctccgaagag tcttgtcata gagatgcaaa ctcaactctc ccgagaaact
     8161 ccttttgagc gccagcattt gaccgagagg aagttggagc accgtcccga catactctgg
     8221 tgcaatcgga agctctcttc cgccacggtc taccaaggct gcaagctgga cggcagcagg
     8281 ccggccatag tcgaacaatt cattcatcgc cgcgcgaatc gtccgtcccg tatagagcac
     8341 gtcatccaca agaatgatgc ttgctccatc gacgtcgaag gggatgtccg acggcttgac
     8401 ttgcgtgtgc aacccgatcc ggtcaaaatc atccctatag aacgagatat ccagcgtgcc
     8461 gaacgggagc gagagcccga gtttgcggtg gagacgttca gccagccatg ctccaccggt
     8521 gtggatgcca accagtgcca gtgccgcgtt cttctcgacg gcagggcgaa gctggtcagc
     8581 aaggctggcg agtaattgct cagcgtcagg cagttccatt gtatcgatcg aaataggatt
     8641 gtaaaatcag ttgagctgcc acctgatcga gcacaggctt ttgctttttt ccttttatac
     8701 ctgcttcccg caatgctgtg ctggcatcga tggatgtata gcactcgtca acgagaacag
     8761 tcttgatccc aaagcgccct tccaggcgtc tggcgaagcg acggcttcgc cgggtcaact
     8821 cgtgttcttt gccgtccgtg tgggtgggta gccctaccac aagcaggatg gggcgccatt
     8881 gccggattaa ctcgaaaatt ctgtcaaatt gctgttcccc attggagccg gttatcgtca
     8941 caagcggatg cgccaggcaa agtccgaggt ttcctaccga cacgccaatt cgttttgcac
     9001 caaaatcgaa accca
//
LOCUS       DASSHJ010000182         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_988300, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000182 DASSHJ010000000
VERSION     DASSHJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988300"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1417
                     /locus_tag="VFI43_07075"
     CDS             395..1417
                     /locus_tag="VFI43_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061925.1"
                     /translation="MDVDQLTDDQIARLTPEQIEILENNPEKLTEILALQQSSRDVPQ
                     ETPSEKPEQEDNSAANGAGSDEGSVEDEAADKDASGTGKEEEPAGVSTKNGKGIIPYE
                     KHKELRVENSTLRQQLREAQSKLDDLLKRKDEVRSVAGAAEADDAIGKYLETLKGNMP
                     ELHQVITAVLEGSRKQGEKLEQTLTELEREKEESERRKQLSVAEQVAEAKDNNPDLVH
                     WESNDPEAWEEALKQDEILRTSSRWANKPYAERFEEVVRRVRAIVPDASPARKQPDPE
                     SVKAEARAKLEKAPARKPTTLSDIHGGVSASPSDEVSMMSPYELARKLMTMPAHKAAA
                     LRAELD"
     gene            1429..2700
                     /locus_tag="VFI43_07080"
     CDS             1429..2700
                     /locus_tag="VFI43_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HET7061926.1"
                     /translation="MAETNVANGSSLAIKHYSAALFANTLKGTTAMENLVGPVDPSAA
                     MEKMAGQTQPGMPVVRIDNLMKNAGDTVSLDLVDTIGGEPLMGDVNREGRGSALSFSS
                     MDIRIDLSSKVVDAGGSMSQQRTKHQLREIALAQLSGYFPRLASQTSLVHLAGARGSQ
                     TGTDWTIPLQSTANFASVMVNPVKAPTYNRHFVVNGSGLTAGGQQLGSIVSTDALKLV
                     HLDLLRKRLDDMDQPLQSVQLVGDRAAQTSKMWVFLATPNQYSVLLTEGSLRAFQQNA
                     VNRSAYLDSRHPLFAGEVGMWNGILVIKNERAIRFLPGDTTKIVTAANAATAVESDQQ
                     INSSLTTGFAVERGLLLGAQALGIAYGKTRVSGMQYGWKEHWYNFESNLEVMGEKVCG
                     HAKIRMSIDDGTGSKVPTDFGVITVDSAVPL"
     gene            2767..3210
                     /locus_tag="VFI43_07085"
     CDS             2767..3210
                     /locus_tag="VFI43_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061927.1"
                     /translation="MATFKATDLNRDRFMGGYGNGWIGFGTVTPTGGASGDIYYPLLI
                     PAGVMVTDVDIVNDKLDSSGSPTLACKVGYAPVNSADGPTSDDAYFAAPGTTLLRNAG
                     RTPLAFQPMKFDKPVYLIVTLSANAATFVPGKVTAIVKGDALGIR"
     gene            3278..>3636
                     /locus_tag="VFI43_07090"
     CDS             3278..>3636
                     /locus_tag="VFI43_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061928.1"
                     /translation="MPQVKYIGLTVKTDSIPGIGLQWHPGQIRNVTAEVAERLFVFTD
                     TWLRIEDKDSDESELVGLTQLPDAIEEPLPVIDFLGMDKTALVTFAQREYNERLDKRL
                     SETTIRHHVIALFGKHQS"
BASE COUNT          882 a          974 c         1055 g          725 t
ORIGIN      
        1 ggggaaagga tttccgtgga aagcagtttt acaatccgcc aaatatagga gcgatggatg
       61 aagtatcagt gaccccgcgc tacctgttca ccgaccggaa gtccgcctga tcaggatttt
      121 acgagcaaca tcttagctga agttgctcca ggtcttctgt tattagcaag ccgcctttgg
      181 gcggcttttt tatttgtgct ttccgctgca tacccggctt gaggtgaaag catttcacct
      241 cgtaacactt gccgatcttt tcgctcatgc gacgcgctca cggcgatacc gcagttttta
      301 tcaacccctg cttagggcct ctgaaaaagt cccacgacaa tgtcagattc cctggcagcc
      361 cgctcactgg gtcaagtagt tggagaagaa ggaaatggac gtagatcagc ttacggatga
      421 ccaaattgca agactgacgc cggaacagat cgagattctg gagaacaatc cggagaagct
      481 caccgagatc ctggctttgc agcaatcatc cagagatgtg cctcaggaga cgccttctga
      541 aaaacccgaa caggaagata acagcgcggc taacggcgcg ggtagcgatg aaggcagtgt
      601 ggaagacgag gctgcggata aggatgcaag cggtaccggt aaggaagaag agcctgccgg
      661 cgtcagcacc aagaatggga aaggaattat tccctacgag aagcataagg aattgcgggt
      721 ggagaactcg accctgcgcc agcaattgcg ggaggcccag agcaaactgg acgacctcct
      781 gaagcgcaag gacgaggtca ggagcgtggc gggtgcggcc gaggcggatg atgccatcgg
      841 gaagtatctg gaaacgctca aggggaatat gccggaactc caccaggtaa ttactgctgt
      901 tctcgaagga agccggaagc aaggtgagaa gctggagcaa acgcttacgg agctggagcg
      961 tgaaaaggag gaatccgaac gccggaagca attaagcgtt gccgagcagg tcgccgaagc
     1021 caaggataac aatccagacc tggtgcattg ggaaagcaat gatccggaag cctgggaaga
     1081 ggcattgaaa caggacgaaa ttttgagaac cagctcccgc tgggcgaaca agccctacgc
     1141 ggaaaggttc gaggaggtcg tgcggcgtgt cagggcaatc gtgcctgatg cctctcccgc
     1201 gagaaaacaa cccgatccgg agagcgtgaa agctgaagca agagcaaagc tggaaaaagc
     1261 cccggctagg aaacccacaa ccctatcgga tattcacggc ggagtgtctg catcgccgag
     1321 cgatgaagtc agtatgatga gcccgtatga actggcgcgc aaactgatga ccatgcccgc
     1381 tcataaggca gccgccttga gagccgaact tgactgagga attattgaat ggctgaaacc
     1441 aatgtagcaa acggcagctc gcttgccatc aagcactaca gcgcggcgct tttcgcgaac
     1501 acactgaaag gcacgacggc aatggaaaac ctggtcgggc ccgttgaccc gtccgctgcg
     1561 atggaaaaga tggccgggca gacccagccc ggcatgcccg ttgtccggat tgacaatctg
     1621 atgaagaacg cgggcgatac ggtgtcgctg gacctggtcg atacgatcgg tggcgagccg
     1681 ctcatgggtg atgtgaatcg cgaaggccgt ggcagcgcgc tttcgttttc ctccatggat
     1741 atccgcatcg atctctcgag caaggtcgtg gatgcgggcg gcagcatgtc tcagcagcgc
     1801 acgaagcatc agttgcgtga aatcgcactg gcccagctct ccggttattt cccgcgtctt
     1861 gcctcgcaga cgtcactggt ccatcttgcc ggtgcgcggg ggtcgcaaac cggaacagac
     1921 tggacgatac cgttgcaaag cactgcgaac ttcgcttctg tcatggtcaa ccccgtcaag
     1981 gccccgacct acaaccgcca tttcgtggtc aacgggtcgg gattgactgc gggcggccag
     2041 caactgggtt ccatcgtttc gacagacgca ctcaagctgg tgcatctgga cctgcttcgc
     2101 aagaggctgg atgatatgga tcagcccttg cagtccgtgc agcttgttgg cgaccgggca
     2161 gcccagacct ccaaaatgtg ggtattcctt gccaccccga atcaatattc ggttttgcta
     2221 accgagggct cgctgcgtgc gttccagcag aacgcagtaa accggtcggc atacctcgac
     2281 agtcgccatc ccttgttcgc cggtgaagtg ggtatgtgga acgggatcct ggtcatcaag
     2341 aatgagcgtg ctatccgctt cctgcccggg gacacgacca agatcgtgac cgcggccaac
     2401 gcggcaacgg ctgttgaaag cgatcagcag atcaattctt cgctgaccac cggtttcgcc
     2461 gttgagcggg gcttgttgct gggcgcccaa gccctcggaa tcgcctatgg aaaaacgcgg
     2521 gtaagcggca tgcagtacgg ctggaaggag cactggtata acttcgagag caatcttgaa
     2581 gtaatgggtg agaaggtttg cggtcacgca aagatccgga tgtccatcga cgatggaacc
     2641 ggttcgaagg tgcctaccga cttcggcgtc atcaccgtcg attctgccgt cccgctctaa
     2701 ccggtcgtga cgtaaggcgg ggacgaacaa ttcctcgcca atctattctt ttccaaggag
     2761 attctaatgg caactttcaa ggcaacggac ttgaatcgcg atcgcttcat gggtggttac
     2821 ggtaatggct ggatcggttt tggcacagtc acgccgacgg gcggcgcgtc aggagacatt
     2881 tactatcctc tcctcattcc ggcgggggtt atggtgacgg atgtggacat cgtcaatgac
     2941 aaactggaca gcagcggttc tcccactcta gcgtgcaagg tcggatatgc gccagttaat
     3001 tccgcagatg gccccacttc cgacgacgcc tatttcgccg ccccagggac tacacttctc
     3061 cgcaatgccg gacgtactcc cctcgctttc caacccatga aattcgacaa gcctgtgtac
     3121 ctgatagtca ctttaagcgc taatgccgca actttcgttc caggcaaagt tacggcgatc
     3181 gtcaaaggcg acgctttagg catcagatag ttatccagct tgcaagagtt caggcggctc
     3241 cggcgaaagc ggagccgcct ttatgaggga gttctaaatg ccccaagtca aatacatcgg
     3301 gctcaccgta aaaacagaca gcattcccgg aatcggattg cagtggcatc cgggacagat
     3361 ccggaacgtg accgctgagg ttgctgaacg cctcttcgtt ttcaccgaca cctggctcag
     3421 aatcgaggac aaggattcag atgaaagcga gctcgtaggg ttgacgcaac ttccggatgc
     3481 aattgaagaa cctcttcccg tgatcgattt cctcggcatg gacaagacag ctctggtgac
     3541 ctttgcgcaa cgcgaataca acgaacggct cgataagcgg ctgagcgaga ctacgatccg
     3601 tcaccacgtg attgcgctgt tcggcaagca tcaatc
//
LOCUS       DASSHJ010000183         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_291497, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000183 DASSHJ010000000
VERSION     DASSHJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291497"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VFI43_07095"
     CDS             complement(<1..1160)
                     /locus_tag="VFI43_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET7061929.1"
                     /translation="MNKTVQPDNAEKPVLNPDELRKINAYWSAANYLSVGQIYLLGNP
                     LLRKPLTVEDIKPRLLGHWGTTPGLNFIYAHLNRAITKYDLDMIFICGPGHGGPAMVA
                     NTWLEGSYSKFYPRISESETGMERLFKQFSFPGGIPSHAAPETPGSINEGGELGYSLA
                     HAFGAVFDNPDLIAACVVGDGEAETGPLAASWHSNKFLTPARDGAVLPILHLNGYKIA
                     GPTVLSRISHEELEELFLGYGYQPYFVEGSDPEPMHEKMAAVVDAVIAKIKSIQKETR
                     ASNAPSPEIYPHWPMIILRSPKGWTGPKEVDGKKVEDYWRSHQVPLAQIRSNPQHLKM
                     LEEWMKSYKPEELFDVQGKLVPELAALAPKGKRRMGANPHANGGLLLKDLEMP"
     gene            complement(1244..2350)
                     /locus_tag="VFI43_07100"
     CDS             complement(1244..2350)
                     /locus_tag="VFI43_07100"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HET7061930.1"
                     /translation="MNTGSSSVRLGAFTNDGGTQKEVASARLERSASSPANPEAALTR
                     FMEAHGFRTFSAAAHRVVHGGKNLTESRLIDEKVEQEIERLAVLAPLHNPVALRWIRA
                     ARQVLGPAVPQVAVFDTAFFTALPEVARIYAIPHELTEQHGLWRYGFHGLAHQAMWLG
                     WREQIPGDSLKGKVISLQLGSGCSITATAGGVPQDTSMGFSPLEGLVMATRSGDIDAG
                     LVTFLEDEKGFGPEQVDELLNERSGLSGVSGISADIRELLSSANDQARLAVDLYCYRA
                     RKYVGSYLAVLNGADAIIFGGGVGENAAVVREKILEGMEWCGIELDQKKNRDHDRKEI
                     SCISREASRVEVWVIPVNEAALLAQEAESVLRNL"
     gene            complement(2362..3273)
                     /locus_tag="VFI43_07105"
     CDS             complement(2362..3273)
                     /locus_tag="VFI43_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7061931.1"
                     /translation="MGKIRRILAATDFSPRARRAVQRAAQIAHEHEAELYLLNVLPVF
                     PLEALKRLMVETPPETEQRLYNQAKAALQQEAELLARNRIVIRQHVAIGRAHIEINRY
                     AKSHRIDLLVIGDQGESFVRELFLGTTASKTLRTGGHPLLITKQEPRGPYKHVLVPVD
                     FSPSSLEALDFASKVVTGEAIYVLHALNIPTERGKERHTSLHEEWIDPYRNKIVNRAQ
                     EDMDGVVEDYASRQIPVTPLIDRGPAPAVIREKARALDIDLIVIGKRGETELDDTLLG
                     SVTKHVLYETTCDVLIVSPATAPNPPG"
     gene            3564..4442
                     /locus_tag="VFI43_07110"
     CDS             3564..4442
                     /locus_tag="VFI43_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HET7061932.1"
                     /translation="MKKILTLFTLAIFSFGLATFDAEAKRFGGGQSVGTQRQSIGSQG
                     APKSTQTPAAAPAPAAAGGNRWLGPLAGLAAGGLLAALFMGGAFEGINMMDVLMLAAL
                     MAAVFFVVRMMRKPRQEQSARPMQYSGMGAGAAGGTTPASGPTVAPSETSGGSEPKAP
                     VPSGTPNIPADFKVEPFLRNAKTSFIRLQAANDARDLNDIREYTTPEMFAEISMQINE
                     RGNAPQKTEVLSINAELLEVVTENDKAIASVRFNGQLREAPQPLPESFDEIWHVQKNL
                     KDPDAVWLLAGIQQVS"
     gene            4455..5030
                     /locus_tag="VFI43_07115"
     CDS             4455..5030
                     /locus_tag="VFI43_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein"
                     /protein_id="HET7061933.1"
                     /translation="MLESVAAALLNHLLRGESWARKRLQPYAGKTARFCLPPFPDLVL
                     IVQATGELSPATGDRVDDTVLIAAPGLLPGLLSGDERAYRKIYISGDREFAEEILHTG
                     RNLNCDAEQELSHLMGDILAHRVVQSGRNLVHWHADTIRNLSQALMDYWTEEHPVLAK
                     PAHLRNFIREVESLQDKTAYLEKRVNALIVQ"
     gene            5170..>5618
                     /locus_tag="VFI43_07120"
     CDS             5170..>5618
                     /locus_tag="VFI43_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7061934.1"
                     /translation="MRFFRLIKIFIVAFRFGLDEFFLSHERLRVFRPAVRLATFWRRL
                     DKARGERLRLALEALGPIFVKFGQMFSTRRDLLPPDIADELAKLQDSVPPFPSDVALK
                     TLELVYGKPVREVFLVFDSEPVASASIAQVHLAVLHDGTEVAVKILRP"
BASE COUNT         1156 a         1622 c         1517 g         1323 t
ORIGIN      
        1 ggcatttcga gatctttcag cagcaatccg ccgttcgcgt gcgggttggc tcccatgcgt
       61 cgttttcctt ttggagcaag tgcagcgagc tcgggaacga gttttccctg cacgtcaaac
      121 aattcctccg gcttgtagct cttcatccac tcttcgagca ttttcaagtg ctgcgggttc
      181 gagcggattt gcgccagagg aacctgatgc gagcgccaat aatcctccac tttcttgcca
      241 tcgacttctt tcgggcctgt ccatcctttt ggacttcgca ggatgatcat gggccaatgg
      301 ggatagatct ctggtgaagg cgcattactt gcgcgagttt ccttctgaat ggattttatt
      361 ttggcgataa ctgcgtctac caccgcagcc atcttctcat gcatgggttc cggatccgat
      421 ccttcgacga aataaggctg atagccatag cccaggaaca gttcttccag ttcctcgtgg
      481 ctgatgcggg aaagcacggt tgggcccgct attttgtagc cgttcagatg gaggatcggc
      541 aggaccgccc cgtcgcgagc gggagtcagg aatttattcg agtgccagga cgccgctagc
      601 ggtccggttt cagcttcgcc atcacccact acacaggcag cgatcagatc cgggttatca
      661 aaaacagcgc cgaaagcatg ggcaagcgag tacccaagct cccccccttc attgattgaa
      721 cctggcgtct cgggcgctgc atggctgggg atgccgccgg ggaaagagaa ctgtttgaac
      781 aaacgctcca tcccggtttc gctctcggaa atgcgcggat aaaacttgct atagctgcct
      841 tcgagccatg tattggccac cattgcgggt ccgccgtgcc cggggccgca aataaaaatc
      901 atgtccagat catattttgt tatggcccgg ttcaggtgcg catagatgaa gttgagcccg
      961 ggagtagtcc cccagtggcc gagcaagcga ggttttatat cctccacggt aagcggcttc
     1021 ctgagtaacg gattgccgag aagataaatc tgtccgacag agaggtagtt tgctgcgctc
     1081 cagtaggcgt ttatcttgcg cagctcatcg gggttcagga ctggtttctc agcattgtct
     1141 ggttgcacgg tcttgttcat acgcttcctc caaactaact tgcttcaact ttctgcctcg
     1201 atagccgctt gcttttcagt acacaacgcc tttgcgggtt caatcagagg tttcttaaaa
     1261 cactctctgc ttcctgtgcg agcagcgccg cttcgttcac tggaattacc caaacctcaa
     1321 ccctgcttgc ttcgcggctg atacacgata tttcttttcg gtcatgatcc cggttctttt
     1381 tctgatcgag ctcgatgccg caccattcca ttccttccag tatcttttcc cgcactaccg
     1441 ctgcattttc gccgacgcct ccgccgaaaa taatcgcatc cgcgccattc aatactgcca
     1501 agtaactgcc cacgtacttt cgcgcccggt aacagtaaag atccaccgcg agcctcgctt
     1561 gatcattcgc cgagctcaga agctccctga tatcagcact gatccccgat acccctgaca
     1621 ggccggaccg ttcgtttagc agttcgtcca cttgctccgg gccgaagcct ttttcatcct
     1681 ccaggaacgt gacgagtcct gcgtcaatgt cgcccgagcg tgttgccatc acgaggcctt
     1741 ccaggggcga gaatcccatc gatgtgtcct gcggcactcc tccggctgtt gcagtgatcg
     1801 agcaacccga gccgagctgc aatgagatca ccttgccttt cagcgaatca ccgggtatct
     1861 gttcgcgcca tcccagccac attgcctgat gggccagccc atggaagccg tagcgccata
     1921 gcccatgctg ttcggtaagc tcgtgcggaa ttgcgtatat gcgcgcaacc tccggcagag
     1981 ctgtaaaaaa cgcggtgtca aataccgcga cctggggaac cgcggggcca agtacttggc
     2041 gcgccgcgcg aatccagcgc aacgcgaccg ggttatgcaa gggtgccagt acagccaaac
     2101 gctcgatctc ctgctcgact ttttcatcga tcaggcggct ttcggtcaga ttctttcccc
     2161 catggacaac acggtgtgca gccgcactaa atgtccggaa tccgtgcgcc tccatgaatc
     2221 gggttagcgc cgcctcagga ttggcaggtg aactcgccga ccgttcgagg cgagcgctcg
     2281 ccacttcttt ctgcgtgccc ccgtcgttgg tgaaggcccc tagccgaact gacgagctgc
     2341 cggtattcac ggtcagaatc atcatcctgg tggattggga gcggttgcag gactgacgat
     2401 caggacatca catgttgttt catacagcac atgttttgta accgatccga gcagggtgtc
     2461 gtccagttcc gtttcgccgc gcttgccgat gacaatcaga tcaatgtcga gcgcgcgcgc
     2521 tttctccctg atgacggccg gcgcggggcc gcgatcgatg aggggtgtaa ccgggatctg
     2581 ccgggaggcg taatcctcga caacgccgtc catatcctct tgcgcgcggt tcactatctt
     2641 gttccggtaa ggatcaatcc actcctcgtg gaggcttgtg tgcctttcct ttcccctttc
     2701 agtcggtatg ttcagggcat gcagtacata tattgcctct cccgtcacca cctttgacgc
     2761 aaaatccagt gcttccagcg aggaaggtga gaaatccacg ggcaccagca catgcttgta
     2821 tggacctctt ggttcctgtt ttgttatcag gaggggatgg cctcctgtgc ggagcgtttt
     2881 tgatgcggta gtaccgagaa acaattcccg aacaaagctc tcgccctgat ccccgatcac
     2941 cagcaggtcg atccggtgcg acttcgcgta acggttgatc tcaatatgag ctcttccaat
     3001 cgcaacgtgc tgcctgatta ctatccggtt gcgggccaac aattcggctt cctgctgcag
     3061 cgctgccttg gcctggttat acagcctctg ttccgtctcc ggcggagttt caaccattaa
     3121 acgctttaag gcctcgagcg ggaagaccgg gagcacgttc aggagataca gctccgcctc
     3181 atgctcatgc gctatttgag cagctcgctg cactgctcgc ctggcgcggg gcgagaagtc
     3241 ggtagctgcc agaatccgtc gaatttttcc cacaagcagg ctccgcgttc gttaacgatc
     3301 acccggcatt tagcgacgcc ggcgcagacg gtttcgggtt gagcgtgcag caagcggcgg
     3361 tgaaattcgg acaggccgcg atccgctgga atcagtatag gtcatgcagg aatgaatgcg
     3421 ccgcgcccaa ctgaggaact tgaccccgtg aggagaatca aacctgtgtc caagctcact
     3481 ggttgtttcg tgatactctc tctcattgaa attatcgctg agtgctatac tttccacttc
     3541 aaccgctatc tctggagatt ctaatgaaga agatattgac cctgttcacc ctggccattt
     3601 tcagcttcgg cctggccact tttgatgcgg aagccaagcg cttcgggggc ggtcaaagtg
     3661 ttggcacgca acgtcaatcg ataggctccc agggggcgcc aaaatcgaca caaacccctg
     3721 ctgctgcgcc ggcgcccgca gcagcaggag gaaacagatg gcttggaccg ctcgccggtc
     3781 ttgccgctgg aggcctgctc gcagcacttt tcatgggcgg tgcttttgaa ggtataaaca
     3841 tgatggatgt gcttatgctg gccgcgctta tggcagcggt attctttgta gtccgtatga
     3901 tgcgtaagcc gcggcaggag cagtcagccc ggcctatgca atattcaggg atgggcgcgg
     3961 gcgcagcggg gggcacgaca cctgcgtcag gcccaaccgt ggccccatcc gaaacctcgg
     4021 gaggctctga accgaaggct cccgttccca gcggaacacc taatatcccg gcggacttca
     4081 aggttgagcc cttcctgcgc aacgcaaaga cctcttttat caggcttcag gcagccaatg
     4141 acgcgcgcga cctgaacgat atccgcgagt acaccactcc ggagatgttt gccgagatca
     4201 gcatgcagat caacgaacgc gggaacgcgc ctcaaaagac cgaggtacta tccattaatg
     4261 cagagttgct cgaagtggtc actgaaaatg acaaggcaat tgccagtgtc cgtttcaacg
     4321 gccagctgcg cgaggcgccg caaccattgc ccgaaagttt cgatgagatc tggcacgtcc
     4381 agaagaatct gaaagacccg gacgctgtct ggctgctggc aggaatccaa caggtttcct
     4441 gagttccacc ctttatgctc gagtccgttg cggccgccct gctcaaccac ctcctacggg
     4501 gggaaagctg ggcgcgcaaa cggctgcaac catacgcagg caagaccgcg cgtttttgcc
     4561 tgccaccttt ccctgacctt gttctgattg tgcaagccac cggcgaattg tcccctgcaa
     4621 caggagaccg ggtcgacgac actgtcctga tagctgcccc tggcttgttg ccgggattgc
     4681 tttccggtga cgagcgcgcc tatcggaaga tctatatctc tggcgaccgg gaattcgcag
     4741 aggaaatact tcataccgga cgcaatctga actgcgatgc ggagcaggag ttgagccact
     4801 tgatgggtga tattcttgcg caccgcgtcg tgcaatccgg caggaacctc gtgcattggc
     4861 acgcagacac aattcgcaat ttgtctcaag cactcatgga ttattggaca gaagaacatc
     4921 ccgtgctggc aaagcccgcc catttgcgta attttatccg ggaagtcgaa tctctgcagg
     4981 ataaaacggc atatctggaa aagcgggtaa atgcgttgat tgttcaataa ttacctcaaa
     5041 agagatagaa aaaccgcctc cggcccggcg gcggtgctag cgcgagttga ggtttcgtgc
     5101 gtcctgaagc aggacgtccc acgcctcgag tggggaaagc attgacgaat tgactattca
     5161 caagaataaa tgcgtttctt tcgtctcata aaaatattca tcgtagcctt tcgttttggc
     5221 cttgacgagt ttttcctgtc ccatgaacgg cttcgggtat tcaggccggc cgttcggctc
     5281 gcaactttct ggcggcggct cgacaaggca aggggggagc gccttcgtct ggctcttgaa
     5341 gcgctcggac ctattttcgt caagttcgga cagatgtttt ccacccggcg cgatcttcta
     5401 ccgccggaca tcgcggacga gctggcaaaa ttgcaagaca gcgttcctcc gtttccgtcc
     5461 gacgtggcgc tcaagaccct cgaactggtt tatggcaagc ctgtccgtga agtttttctg
     5521 gtgttcgatt cggagcccgt cgccagtgct tcaattgctc aggttcatct ggctgtactg
     5581 cacgacggaa cggaagttgc ggtaaagatt ctccgtcc
//
LOCUS       DASSHJ010000184         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_471366, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000184 DASSHJ010000000
VERSION     DASSHJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471366"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFI43_07125"
     CDS             <1..1032
                     /locus_tag="VFI43_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HET7061935.1"
                     /translation="VASRILWQIAVQPYETEADVYSIVRALPWEQWFSPALTIRVNLA
                     AIRCPLRSLDFVTLKVKDAVCDKFRETTGRRPNVDTAKPDIRIHGFFDAQMLSLYLDT
                     SGEPLFKRGLRKSAGEAPIRENLAAGILYLAGWKPGTPLLDPMCGSGTFLLEAAQIAL
                     AIAPGSGRSFAFEKLRNFDAECWSRLKEMAAARQRAKIPQPIFGSDLYGDALAVAREN
                     LSAAGLSEVVMLKQANVLEISAPAAAGILITNPPYGVRMGNRQPLEDFYRKLGDVLKK
                     KFSGWNAYIMTADPLLPKLIRLNPSRRVPLFNGSLECRLLEYKIVAGGMRKQRANSET
                     AKTSGEPDE"
     gene            1046..1540
                     /locus_tag="VFI43_07130"
     CDS             1046..1540
                     /locus_tag="VFI43_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HET7061936.1"
                     /translation="MKLRAVRGIFLWIFLGCTGLIAYALYLQLVKGLLPCPLCVVQRV
                     AYWLIGLTALMAFFHPSRVARRVYSALIALFAFSGATVALRQAWLVRYPEAFECGISP
                     EEEFLNALPLARWWPSMFEANGDCADVSWKFASLTLPDWSAVFFAIFCVLGVYLFSTP
                     QERH"
     gene            1709..2587
                     /gene="htpX"
                     /locus_tag="VFI43_07135"
     CDS             1709..2587
                     /gene="htpX"
                     /locus_tag="VFI43_07135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HET7061937.1"
                     /translation="MKRIFLFVLTNLAILLMLSITLRLLGIDRILNAEGTGLDLNSLL
                     VMAAVIGFGGSFISLAMSKWSAKNMTGAVVIEQPSDPTERWLVETVRRNAERVGIGMP
                     EVAIYDSPDLNAFATGMNRNDALVAVSTGLLHNMSKDEVEGVLAHEISHVANGDMVTL
                     ALIQGVLNTFVIFLSRMIGHLVDRVVFKTEREHGPAFMIVTLVAQLVLGVLASMIVMW
                     FSRQREFRADAGGAQLAGRNKMIAALERLKQWHEPTQLPDRLAAFGIRGQGGIAELFM
                     SHPPLDVRIAALRAMP"
     gene            complement(2698..>3571)
                     /locus_tag="VFI43_07140"
     CDS             complement(2698..>3571)
                     /locus_tag="VFI43_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HET7061938.1"
                     /translation="DDAPNKTSASKNSTTDSSATAAASTPEPSSVTPGATNSNKVAAV
                     DNSSAPNRNNGSASNGPVFKNGAPPDSYPTVNKKDPWESMNRSVFSFNEKIDENVLKP
                     VARGYKWIMPDPLETAVGNVFSNLNDIPVTFNNILQLKLRNALRSSGRFVVNSTVGLG
                     GMVDVASDIGLEKQNEDFGQTLGYYGIAPGPYLVLPVLGPSSTRDAGGLPIDFATDPI
                     AVGSFFVAPFIGPVVGSTRFADTRARLLDNDKTLNEASLDKYEFMREAYLQRRRNLVH
                     DGNPPPHKDDEDLE"
BASE COUNT          766 a          929 c          999 g          877 t
ORIGIN      
        1 gttgcgagca ggattctgtg gcagattgct gtccaacctt atgagaccga agccgacgtg
       61 tacagtatcg tccgtgcgct tccatgggag cagtggttct ctccggctct taccatacga
      121 gttaatctgg cggcgatcag atgtccatta cggagcctcg actttgttac cctcaaggtg
      181 aaggatgcag tctgcgataa gtttcgggag acgaccggtc ggcgacccaa cgtagataca
      241 gcgaagcctg atattcggat tcatggtttt tttgatgcgc aaatgctttc actttatctc
      301 gatacctccg gtgaaccatt attcaagcgc ggtctgagaa agtcggctgg agaggcaccg
      361 atacgagaaa accttgcagc aggaatcttg tatctggcgg gatggaaacc aggcactcct
      421 ctgctcgatc ccatgtgcgg cagtggcacc tttctgctcg aggcagcgca gatcgcgctg
      481 gctatcgctc ccggatcagg acgctccttt gctttcgaaa aattgagaaa ctttgatgcg
      541 gaatgctgga gccgcctaaa ggaaatggca gcggcccggc agagggcaaa aataccgcag
      601 ccaatattcg gcagcgatct ttatggtgat gcattggctg ttgcccgtga gaatctctct
      661 gccgccggat tgtcagaagt cgtgatgctg aagcaggcga atgtgcttga gatttcggct
      721 cctgcggcag caggcatcct tatcaccaat cctccctacg gagtgcgcat gggcaaccga
      781 caaccgcttg aagattttta tcgaaagctt ggagatgtac tgaagaaaaa attcagcggg
      841 tggaacgcat acataatgac cgccgatccc cttctgccga aactcatacg gcttaatccc
      901 tcgcggcgcg ttcccttgtt taacggatca ttggaatgcc gcctgctgga atacaaaatc
      961 gtagcgggag ggatgcgaaa acaaagagca aacagcgaaa cggctaaaac ttcaggcgaa
     1021 ccagacgaat aggtggcaag gagtaatgaa gctgagggca gttagaggaa tttttttatg
     1081 gatatttctg ggctgcacgg gtctgatagc ctatgctctt tacctgcagt tggtaaaggg
     1141 cctgttgcct tgcccgctct gcgttgtaca gcgggttgca tactggctca tcgggttaac
     1201 tgcgctcatg gccttcttcc atccctcgcg ggtggctcgc cgtgtttaca gcgcgttgat
     1261 cgcactcttt gcgttctcgg gcgccacagt tgctttgcgc caggcatggc tggttcgata
     1321 ccccgaagcg tttgagtgcg gcatcagtcc ggaagaagaa tttctgaacg ctttgccgct
     1381 tgcgcgatgg tggcccagca tgttcgaagc gaatggcgat tgcgcggacg tttcctggaa
     1441 atttgcctcg ctcacccttc cggattggtc tgccgttttt tttgccattt tctgcgtttt
     1501 gggggtgtat ctgttttcaa ctccacaaga acgccattga ccatagagaa tgaacgcccg
     1561 gtgcaatacc gaagttttat ataaactgat cagacgggat aaaagataag cgaggtaaaa
     1621 gataatttag cgaacgttcg ctccttcttg aaaacgaaac tttcaaccct atctataacc
     1681 tgtctttaaa caaagaacgg accgatccat gaaacgcatt ttcctttttg tactcaccaa
     1741 cctggccatc ctcctgatgc tgagtattac cctgcgcctg ctggggatag accgcatcct
     1801 gaacgcggag ggtacaggcc ttgatttgaa ctctctgctg gtcatggcgg cggtgatcgg
     1861 ttttggcggc tcattcattt cgcttgcgat gtcgaagtgg agcgcaaaga acatgacggg
     1921 agcggtcgta atcgagcagc cttccgaccc gacagaacgc tggctggtgg aaacggtccg
     1981 gcgtaatgcc gagcgtgttg gaataggcat gccagaggtc gcgatttatg attcgccgga
     2041 tctgaatgct tttgcgaccg gtatgaaccg taacgatgct ctggttgcgg tcagcaccgg
     2101 cttgctccac aacatgagca aagacgaggt tgaaggcgtg ctagcgcacg aaatcagcca
     2161 cgtagcgaac ggggacatgg ttacgctcgc gctgatccag ggcgtgctca atacatttgt
     2221 gatattcctg tccaggatga tcggccacct ggtagatcgg gttgtcttca aaacggaacg
     2281 ggagcacggg ccagccttca tgatcgtgac gctcgttgcc caactggtac tgggcgtgtt
     2341 ggccagcatg atcgtgatgt ggttcagccg tcagcgcgag tttcgcgccg atgccggcgg
     2401 cgcgcaactg gctgggcgaa acaagatgat cgccgcgctt gagcggttga agcagtggca
     2461 tgagccgact caattgccgg accggctggc cgcatttggc atccgcgggc agggcggtat
     2521 tgccgaactc ttcatgtcac acccgcccct ggacgtccgt atcgccgcgc tgcgggcaat
     2581 gccctgatcc tcctctttcc atataaaaaa ggcagagctc gctctgcctt ttttatttcg
     2641 cctgttcgct gttcaggaac aatttaccaa gcctgcctca actcctcggc ccgtaatcta
     2701 ctccagatct tcatcatcct tatgcggagg tggatttccg tcgtgtacga gattgcgacg
     2761 ccgctgcaga taagcttcgc gcatgaactc atatttatcc agcgacgctt catttagagt
     2821 cttgtcgtta tcaagtagcc gggctcgagt gtcggcaaac cgggtcgatc caaccactgg
     2881 gccgatgaac ggagcaacga agaagcttcc tactgcaatg gggtcagtgg caaagtcaat
     2941 cgggaggccg cccgcatcgc gagttgaact tggtccaagc accggcagta cgagatatgg
     3001 ccccggggct atgccgtagt aacccagcgt ctggccgaaa tcttcgtttt gcttttccag
     3061 tccgatgtcg ctcgctacat ccaccatgcc acccagccca acggtgctgt tgacgacgaa
     3121 ccgcccactg ctgcgcaggg cattgcggag cttgagttgc aggatgttgt tgaacgtcac
     3181 gggaatatca ttcaggttgg agaagacatt ccctacagcc gtttcgagag gatcgggcat
     3241 gatccatttg tagcctcttg ccactggctt caacacgttt tcatcaattt tctcgttaaa
     3301 cgagaatacc gagcggttca tcgattccca cgggtctttt ttattgactg tcggatagct
     3361 gtcgggaggt gcgccgttct tgaaaacagg accgttgctc gcggaaccat tgttccggtt
     3421 cggcgcgctg ctgttgtcaa ctgcagcgac cttgttggag tttgtcgcac ccggggtcac
     3481 gcttgaaggc tccggtgtgc ttgcagcagc agtcgcgctt gagtccgtgg ttgagttctt
     3541 ggaggcgctc gttttgttcg gagcatcgtc a
//
LOCUS       DASSHJ010000185        17084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_354279, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000185 DASSHJ010000000
VERSION     DASSHJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 17084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17084
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354279"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VFI43_07145"
     CDS             <1..1190
                     /locus_tag="VFI43_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7061939.1"
                     /translation="PWFTASGEYAGHVGISLDITEAVNGEIALLEADRRKDEFLATLA
                     HELRNPLAPIVNALTLIAQTDGPAAVPRLLPMISRQVNYMVRLVDDLLEISRITGGKV
                     ELRTVPTDLVGVVRNAIEANMPGINEKQLELSVSLPQTPLVVQGDAVRLEQVFTNLLN
                     NAVRYTPNCGHIWVVAWQEDNSAVVLVRDNGIGILTGMLPRLFEMFSQERRGGMGTQE
                     GLGIGLCLVDRLVKMHGGTVEARSEGKDKGSEFIVRLPLGQTRTSEKMQQPEKTASVK
                     PGLRVLVVDDNHDAAEVLCMLLQSIGVKVEAVDSGSAALAAIPDYRPNVILMDIGMPG
                     MDGNEVARRIRQQPEFNDIKLIALTGWGQEEDRRRSKESGFDHHLTKPVNFRDLADLI
                     ASI"
     gene            2091..2417
                     /locus_tag="VFI43_07150"
     CDS             2091..2417
                     /locus_tag="VFI43_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7061940.1"
                     /translation="MKSSAHIAGAGGIGIAAAACLVRAGWDVTMIELNSAKITVGRLN
                     EMWLDDQRIEGVRFIHFDEWVPPTDEVVLLCTKTFDNAPVLGVSEFLCARRSQTFPRR
                     RSQPLI"
     gene            complement(2457..2677)
                     /locus_tag="VFI43_07155"
                     /pseudo
     CDS             complement(2457..2677)
                     /locus_tag="VFI43_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2677..2967
                     /locus_tag="VFI43_07160"
                     /pseudo
     CDS             2677..2967
                     /locus_tag="VFI43_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3125..3589
                     /gene="traD"
                     /locus_tag="VFI43_07165"
     CDS             3125..3589
                     /gene="traD"
                     /locus_tag="VFI43_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HET7061941.1"
                     /translation="MLHDKWLTARLAYLRGLKAPSEQQQQLLLLADKLSRTDADSRIL
                     TALIRAEATAERAQRAKAEAARLAAGDEEAIRILEANNREAASRERDEELYNVSELLV
                     LAGLVNPATRAAAFDRGELLGALLGLTNVPPEDPRRREWKRAGDALLAQKTK"
     gene            complement(4061..4645)
                     /locus_tag="VFI43_07170"
     CDS             complement(4061..4645)
                     /locus_tag="VFI43_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061942.1"
                     /translation="MLKKMLRLLPLITIFPLAACVSVPTGPSIMVLPGNGRSFEEFRF
                     DDYTCRQFALEQGGAPSRASMSSGLGSAAVGAGLGAAAGAALGGGQGAAIGAGTGAAA
                     GGLAGANAAGTSGSITQQRYDMGYIQCMYAKGHRVPVSAQLNYGPARQAAPYASTPSA
                     STPPPPPPRGGKPQALPPGVPPPPPGLPPPPPPR"
     gene            complement(4655..5110)
                     /locus_tag="VFI43_07175"
     CDS             complement(4655..5110)
                     /locus_tag="VFI43_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061943.1"
                     /translation="MKKIGYLCVILALLGSIPPNPAWARGWGHHHHGGGLGLGLGLGF
                     LGGWGLGYYGRVPYAPYYGYPPAYGVGPYGYGYAPGYGYAPGYGYPPVVSVPAAPPVI
                     VQQQPQVIQAPPPAPPTNYWYYCRNPDGYYPYVKSCPGGWLQVAPQPAQ"
     gene            5631..6083
                     /locus_tag="VFI43_07180"
     CDS             5631..6083
                     /locus_tag="VFI43_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061944.1"
                     /translation="MKKMKLMCVSLMLLGSALAGPVWANGGHHGHGHHGHGHHRHHHG
                     GGGWGYGGYGAVLGMGLLGYGVGSYFGRPSYYGGYTPAYGYAPAYGYAPAVAAPAVPP
                     PVYVQRQDVVHVQAQPQASNYWHYCRHPEGYYPYVKSCPGGWMQVVPQ"
     gene            6286..6606
                     /locus_tag="VFI43_07185"
     CDS             6286..6606
                     /locus_tag="VFI43_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061945.1"
                     /translation="MYKGMTALLAVIVLVYGGAAMARGNGDQHGSGQLGGGGQHGNQG
                     GMASGHMSGEGRDNTNAQWSGGATKGQDRSDLKGGDHQGHGKNHSQNHGKGKGHGQGH
                     GKHQ"
     gene            6701..7306
                     /locus_tag="VFI43_07190"
     CDS             6701..7306
                     /locus_tag="VFI43_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061946.1"
                     /translation="MATESVNYWKFATIGILLVGATVAATLLVAGRDSKTGSSDAATV
                     QMNEPPIDANTQIPPGKVASSEPPRNARVNQSSKASSGALPPPPPYPPQAAYPPQVAP
                     QPAYPPQSVMDGCNRYAHEKVSSKAEEVLKNAALGAAIGAAVGAAGGAIAGGGSGAGK
                     GAAIGGVTGAAGGTLYGLNESKSQDSQYQAAYASCMRSKGF"
     gene            complement(7452..8603)
                     /locus_tag="VFI43_07195"
     CDS             complement(7452..8603)
                     /locus_tag="VFI43_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7061947.1"
                     /translation="MSEPIIIIGSGLAGYTVAREFRKLDAETPLLIVSSDHGGFYSKP
                     TLSNALATGKTPATIVTASAEKMAEQLKATVRSHHRLAAIDPHNKAVALEGGERLSYR
                     ELVLALGADPVRLPLEGNAADEILSVNDLDDYQRLRDALEGKKRVTILGGGLIGCEFA
                     NDLVAAGYQASIIDISNQPLGRLLPPPAGAFLQRKLEAAGVRFYLGEATERVERIGEN
                     FRLTLASGKTIETDLVLSAVGLSPRTTIAKAAGIHVNRGIAVNRSLQTHHASVYALGD
                     CAEVEGRVLPFVMPIMHAARGLAATLAQNHTLVKYPAMPVLVKTPACPTIVAPPESTA
                     QGEWVIEEDETGLKALFKSADGKLLGFALMGSATRERAALSAQLPAIME"
     gene            9175..10095
                     /locus_tag="VFI43_07200"
     CDS             9175..10095
                     /locus_tag="VFI43_07200"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET7061948.1"
                     /translation="MANIGFIGLGIMGKPMTRRLIKGGHTLFLYSRSGVPEELLKQGG
                     NACPSPVEVAENSDVIITMLPEAKDVEQVLFGDRGIACGLGSALPNADLHEPKTIVDM
                     SSISPIETRHFAERIGRLGCHYMDAPVSGGDIGARNGTLTIMVGASEEVFQRVLPVLE
                     LMGQTITRIGNIGSGQICKISNQIIVALTIEAVGEGLLFASKAGADPVKVREALLGGF
                     AASRVLDVHGGRMIRNAFEPGFRVELHQKDLGIALSNARAMGISLPNTASVQEMFNAC
                     VAHGDSKLDSSAVVKVLEKLANHEIQPGQL"
     gene            10092..11546
                     /locus_tag="VFI43_07205"
     CDS             10092..11546
                     /locus_tag="VFI43_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7061949.1"
                     /translation="MSSLDLAARLRDFLPPEAVLQEVEDLKPYECDGLSAYRRIPLLV
                     ALPSTEEEVARVLRVCHSLKVPVVARGAGTGLSGGALPHAEGVLLSLAKLKRILEIDP
                     KARTARVQPGVRNLAISEAAAPYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
                     VHNILRLRVLTAEGELLEIGSEGLDSPGYDLLALMTGSEGMLGIVTEIIVKLMPRPEK
                     AQVIMAAFDDIRKAGDAVADVIAAGVIPAGMEMMDRITTRAVEEYLNAGYNLSAAAIL
                     LCESDGTAEEVAEEIQQIDEIMNRNGATEIKASCNEPERLRFWAGRKAAFPAAGRISP
                     DYYCMDGTIPRKKLAQVLAGIEALSAEYGLRCMNVFHAGDGNLHPLILYDANNPGELA
                     RAEEFGARILQMCIDAGGTITGEHGVGIEKIDQMCSQFKSPELEMFHAVKAAFDPDGL
                     LNPGKAVPTLRRCAEHGAMHVHRGEEKFSELPRF"
     gene            11602..12696
                     /gene="glcE"
                     /locus_tag="VFI43_07210"
     CDS             11602..12696
                     /gene="glcE"
                     /locus_tag="VFI43_07210"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HET7061950.1"
                     /translation="MQDMIDQFSDTIRVAVLRRTALRIRGGGTKDFYGNSITAVGDAS
                     SENEILDPSSFAGLVDYEPTELVVTARSGTRLADLEGALHEHGQMLPFEPPHFGSEAT
                     LGGCVGAGLSGPRRASAGAVRDFVLGVRMLDGKADDMQFGGRVMKNVAGYDISRLLTG
                     SMGTLGLLLEISLKVLPLPPRELTLYFRMSESEAIKKMNEWAGRPLSISATCFTDGEL
                     AVRLSGAEPAVRAARQKLGGEAIAEGGAFWSAVREQTHPFFQRGGQLWRLSIKSTAPL
                     LSLPGKQLIEWGGALRWLMLDEDPDQESVHSAVRQAAESAGGHATLFRSPGPRSNVFH
                     PLSPAMARITQSLKEKFDPHRIFNPGRMYP"
     gene            12704..14035
                     /gene="glcF"
                     /locus_tag="VFI43_07215"
     CDS             12704..14035
                     /gene="glcF"
                     /locus_tag="VFI43_07215"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HET7061951.1"
                     /translation="MQTNLADFIKDTPEGQEAEEILRSCVHCGFCLAACPTYQLLGDE
                     LDSPRGRIYLMKQVLEGAPVTEKTQLHLDRCLTCRACETVCPSGVRYGRLVDIGRDIV
                     EKKVGRDRSSGAVRYALRQVLPKPGVFRTLFKLGQTARPLLGLLSVQLRESIPQSKGT
                     QSTSFWPPPRHPRKMLVLDGCVQPTLAPNINAAAARVLDRLGVSLIKASDAGCCGGLS
                     YHLNAQQEGLDYMRRNIDAWWPYIAGQSSGARGPGEGVEAIVITASGCGVTVKDYGHL
                     LRHDAEYAGRAAHVSAMAKDISEVIDAETETLLPLLKEGTLLEDEKQKLAFHSPCTLQ
                     HGMKIRGVVERILREAGFELTYVPDAHLCCGSAGTYSILQPELSKQLLANKVAALECG
                     APARIATANIGCLKHLQSGTRFPVDHWIEILDERLQHDDEKAQIAAGPIPA"
     gene            14156..15310
                     /locus_tag="VFI43_07220"
     CDS             14156..15310
                     /locus_tag="VFI43_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7061952.1"
                     /translation="MTLSTDQNIDPERLSPAARRMLELRDEVFAEWIKRVRQTVKEAE
                     QLSQPILINTFPSLYENLAEAITPDYPRATGDEGNTVATEHGGERARLTNYNARSVIA
                     EYQLLRWTTFDVLKLNDVQLNDDEVFVINASIDGSIREAVTAFALAQTVLRERFAATL
                     AHDLRNPLSAAHVLAQLIQRSSDLGKIKEFAGQITDNLGRVDRMIQDLLDAVTFQTGE
                     RLPLRLEEFDVQEVAKEVCDQFESVHGPRFRFIGKTVIGWWDRDAIRRALENMIGNAV
                     KYGAPGTPIRIKIDPIDERMLLSVHNEGEPIPPEQIECVFQVFRRAVAAKEDKEGWGI
                     GLPYVRSVAESHGGSVGVDSSAERGTTFLIDIPVDARPFQDAPTLGRKPD"
     gene            15685..>17084
                     /locus_tag="VFI43_07225"
     CDS             15685..>17084
                     /locus_tag="VFI43_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7061953.1"
                     /translation="MSFTSKPMAKKTSSSPLPPRLARLLRESGGIALLGVMLYLALIL
                     YGYDRVDPGWSHTGEGALQNPGGTAGAWLADLLLYLFGASAWWCILFLAYLISLIFRG
                     GESNGVFDRRPLLVSAIGFLVLLLASSGLESLRFGTHKLPLPQAPGGVLGLVISTNLS
                     QALGFIGATLALLILVAIGFSLFTGLSWLRFVEKLGAMLEGACVSAKERWEAWQDRRA
                     GAISAVKRDALVEVGKKRLHEHPPLHIEQPASLIPKSPRVLKEKQAPLFLDLPDSPLP
                     PLHLLDQPAHKEMEVLSPETLEFTSRLIERKLMDFGVEVKVVTAFPGPVITRYEILPA
                     VGVKGNQILNLVKDLARSLSVVSIRVVETIPGKTSMGLEIPNPKRQVVRLTEILSSQA
                     YADMGSPLTIALGKDIGGHPVVADLARMPHVLVAGTTGSGKSVAINAMILSLLYKATP
                     EQVRLILVDPKMLELSV"
BASE COUNT         3865 a         4462 c         4859 g         3898 t
ORIGIN      
        1 tgccatggtt taccgcctcc ggagagtatg cggggcatgt cggcatatcc ctggacatta
       61 ccgaggcggt taacggggaa atagccttgc tggaggcaga ccgacgcaag gacgagtttc
      121 tcgccacact ggcgcatgag cttcgtaacc cgttagcacc catagtcaat gcactgacgt
      181 tgattgccca gacggacggc cccgcggccg ttccccgtct gttgcccatg atcagccgtc
      241 aggtcaacta catggttcgt ctggttgacg atctgctgga gatatcccgc attaccggtg
      301 gaaaggttga actgcggaca gtgccgacag acctggttgg tgtcgtgcgt aacgcaatag
      361 aggcaaatat gcctgggatt aatgagaagc aactcgaatt gagcgtatcc cttccccaaa
      421 cacctttggt tgtacaaggg gatgccgttc ggctcgagca ggtctttacc aatctgctta
      481 acaatgccgt acgctacacc cccaactgcg gacatatttg ggttgttgcc tggcaggaag
      541 acaatagcgc tgtcgtattg gtaagggaca acggcatcgg aattttgacc ggaatgttgc
      601 ctcgattgtt cgaaatgttc tcacaggagc ggcgtggcgg tatgggcacc caggaggggc
      661 tgggcatcgg gctctgtctg gtggaccgcc tcgtcaaaat gcacggtggt acggtcgaag
      721 cccgaagcga agggaaggat aaaggcagcg aatttattgt gcgccttcca ctaggacaaa
      781 cccgaacgag tgaaaaaatg caacaaccag agaaaacggc ttccgtaaag cctggcctgc
      841 gtgtattggt tgtcgatgac aatcatgacg ctgcggaagt cctttgcatg ctgcttcagt
      901 caattggggt caaggtggaa gcagtggata gcggctctgc tgcacttgcg gccattcctg
      961 attatcggcc gaatgtgatt ttgatggata tcggcatgcc cggcatggac ggtaatgagg
     1021 tagcgcggcg gatccgccag cagccggagt tcaacgatat caagctcatt gcgctcactg
     1081 gctggggcca ggaagaggat cgacggagat cgaaagagag tggcttcgat catcacctta
     1141 ccaagcccgt gaatttccgc gacctggcag atctgattgc ctcgatttaa gccgctgatt
     1201 accgatcgag cgtcgcccaa ctattggatc catctaatcc cgcagtgctt tatgtcagca
     1261 tagccaattt tcattttcac tccctcaaca agttattatt cccctgaaac tcatccgtcc
     1321 tttggtttat cggagaaagt atttcaagat atagtttttg ggatactaaa atgcgggaaa
     1381 taacatgaat cttcttgtac cgagctttgt tcgaaaaacg agggtttaag agaaaagaat
     1441 tattggcgga attaacgtgg ttacattaga taccgatact cagaagcgat acctgagcgc
     1501 gcacttgagg aaatggcaga cggctgcggg agctgactga cctcgtcggc gacgagcttg
     1561 aaaacctccg aaaggcggga tgcgccactt caacgtcggc aaccactaca cccggcttcg
     1621 gcttccaaga tcgcgcgagc ccataccttg atcatatgct ccgtcacatc gatcctgcca
     1681 caaaatatct gaaatggcgc ctctgttttg ccagcttccg tttaccttag cagaacagtt
     1741 ccgggaaaga atttattgtg tttgcgtgcg aacagattat gcagtcattt tgaatgtaaa
     1801 taaaaccttt ggtcgtccgc tgtacatgtt tagagcacga cagcttgtat gaaagtcaaa
     1861 atctccagtt ctgcttgcca ggaagctttg ttttaatccg cctctaggga aagtgaactt
     1921 gccgaagcgc ctcgataaca ttttcctgaa aatcctcggt cggggttttg ctattcagca
     1981 atgacttgac cgcgacagta acaacatgcc ccccaccaca ttatgagtgg aaaagttttg
     2041 aaattgtcca gagctctcga ctctcaacct gggtgaggaa gcgctttcat atgaaatcat
     2101 ccgctcatat tgctggcgcc ggaggtatcg gtattgctgc agcggcgtgt cttgtgaggg
     2161 ctggctggga tgtgaccatg attgaattga actctgcgaa gattaccgtc ggtcgcctca
     2221 atgagatgtg gctcgatgac cagcgcattg agggagtgcg cttcatccat tttgacgaat
     2281 gggtcccgcc aacggatgag gtggtgttgt tatgcacaaa aacattcgac aacgctccgg
     2341 tgctaggagt gtcagaattt ttgtgtgcga ggcgttcgca gacttttcca cggcgacgct
     2401 cgcagcccct tatatagggc aagagcgtgc ggcgcagtgg aaaagtcgat ctcattttat
     2461 cgcattgacc tgaaacgatc ttcaaagaga atggcgaact ggttcatcgc ctgtttccag
     2521 tcatgcacgc ccgcccccaa tcggcggtaa tgttgcgcaa tgccagaaag atcagcttgg
     2581 cagctgcctc atcactgggg aaatgactgc gcgtcttgat gatcttgcgc aggcgcgcat
     2641 gcacgctctc gatcgcgttg gttgtgtaaa tgacacgtgt accctgtggt gttcttcgat
     2701 gccttgcggg tgaagatccg ggaagatgcc gtggtgcgca acaaggcgat ttacctggcc
     2761 ctcggcgtgc tgccggacgg cacacgcgac atcctggggc tgtggatcga gaacaccgag
     2821 ggtgcgaaat tctggatgaa ggtattcaat gatcttaaga cccgcggcgt gaacgatatc
     2881 ctgattgcag tcaccgatgg cttgaagggt atggccgagg cactttgtac agtctatcct
     2941 gccacaacgc tacaaacctg catcgtgcta gcttggctac aagcggaccc tcagtagccc
     3001 tgataacttc cagaggcaaa tctgctcata tacccctgga gtggcgtttg ccgtctccag
     3061 cttactctcg cctccctcaa agcagctaga atggagtcgt caatcaacaa accaggaaat
     3121 actcatgttg cacgacaaat ggcttactgc caggctggcg tatctacgtg gcctcaaagc
     3181 accaagcgaa caacagcagc aactcctctt gctggcagac aagctcagcc gcactgacgc
     3241 ggactcgcgt atccttacag cgttgattag ggcggaggcg acggcagagc gggcacaaag
     3301 ggcaaaggcg gaagccgccc gccttgcggc aggggacgag gaagcgatac gtattttaga
     3361 agcgaacaac agggaggccg cctctagaga acgtgacgag gagctgtaca acgtttccga
     3421 gttgctggtt ctggcaggtc tggtgaatcc ggcaacccgc gctgcagcat tcgatcgcgg
     3481 ggagctactg ggagccctgc tggggctgac aaatgtgccg ccggaggatc ctagaagacg
     3541 cgagtggaaa cgtgcgggag atgcattgct cgctcagaaa acaaaataag aatgcggtgc
     3601 gctgaattgg gagaagctaa tgcatctttt attattgata aaatacgtta ccaataatag
     3661 aagagggcga atgagcacct cggtcttccc gcttcaattc ctccttgcag ccggcttctg
     3721 agcacggctg acttccatca actggcggca ccctcctcat tgttcgaata tctatgcgac
     3781 cgaaatgccg tgacacacaa tccggtcaaa ggcatcaagc ggcctttagt ggaaggttat
     3841 caaggcaaga cgccgcaatc atgggggtct gctggaggct cccgctacgt ctcactcagg
     3901 cacgcccgac cgtgtcatct cgggccacct tactctatca cgcactcctg cctgaagagc
     3961 tatgcaagct cttggtcaag gatttcaaac acgaacgtgg tgtcgcatcc ccccgtccac
     4021 ccagataggt tagatctagc tcctggcgct gccacttaac ttagcgcggt gggggcggcg
     4081 gaggaaggcc aggaggagga ggcggcacgc cagggggcag cgcttgtggt ttgcctcctc
     4141 gcggtggtgg cggagggggg gtggacgcgg aaggagtaga cgcataggga gcggcctgac
     4201 gggctggacc atagttgagc tgagctgata ctggcacgcg atgacctttg gcatacatac
     4261 actggatgta acccatgtca taacgctgct gggtgatgct tcccgaagtc ccggcagcat
     4321 tcgcgccggc caagccacct gccgccgccc ccgtaccggc gccaatcgcg gcgccttgtc
     4381 ccccaccgag ggctgccccc gctgctgcgc caagccccgc gccaacggct gcgctcccaa
     4441 gtccgctgga catcgaggcg cggcttggtg ctccgccctg ttccagcgcg aattgcctgc
     4501 acgtatagtc gtcgaaacgg aactcttcaa aacttcgacc gtttcctggc aacaccataa
     4561 tgctggggcc ggtcggtacg ctcacgcacg ccgcaagcgg aaatatcgtt atcaggggca
     4621 ataatctgag catttttttt aacatatctt ttcgctattg ggcaggttgg ggtgcgactt
     4681 gcagccagcc gccggggcaa cttttgacat agggatagta gccgtccggg ttgcggcaat
     4741 agtaccagta attcgtgggg ggagcgggcg gcggagcctg gatgacctga ggttgctgct
     4801 ggacaatcac cgggggcgcc gccggtactg atacgactgg cggataacca tagcccggcg
     4861 cgtatccata cccgggcgcg tatccgtagc cgtacggccc aaccccataa gccggtggat
     4921 atccatagta gggagcatag gggactctgc catagtatcc aagcccccat ccgcccaaaa
     4981 atcccaggcc cagtccaagc ccaagtccgc ctccatgatg gtgatggccc cagccacgtg
     5041 cccacgcggg atttggcgga atgctcccca acagcgccag aataacgcac agatacccga
     5101 tctttttcat gtcaatctcc aaccctccag tattaagatg agttaaccgc cacttcattc
     5161 agctcatgaa atttacgaag gcattcagtc gttagatagt tgttagacag ttattttttc
     5221 aatataacgc gcgaatagag cagaggttgt cacgtgttcc cctaactggc tcgattttgc
     5281 ctacagtcat agcgaaacca acagacttgt atacatgcaa ctgccgttga cgaattacca
     5341 gctctaatcc agttgccatt aaatctggag cacgccttcc ttctctccac ccgtccggat
     5401 aagcggtgtg tcgaaagaag cggcgaattc tcactgggtc ctgcctttgc cttgactcgg
     5461 cgaacgctat tttcgattac tggaatcagc cctatcaaaa aattttcata gagcgcgttg
     5521 caagacaacc aattttcggc tcatgcgtta ttaccattgc aactgcataa atcccgctta
     5581 taagcggctt ttattccgca gggcgactta accacaagtt ggagactgat atgaaaaaaa
     5641 tgaagttaat gtgcgtgtcc ctcatgctgc ttggatcggc ccttgccgga ccagtttggg
     5701 caaatggcgg gcatcacggt cacggtcatc acggtcacgg acatcaccgc catcatcacg
     5761 gcggcggtgg ttggggatac ggtggatacg gagcagtgct gggaatggga ttgctgggat
     5821 atggtgtggg cagttatttc ggacgtccgt cctattatgg tggctatacg cccgcctatg
     5881 gttacgcgcc tgcctatgga tatgcaccgg ctgttgcagc gcccgccgtt ccaccccctg
     5941 tttacgttca acggcaggat gtcgtgcacg tccaggccca gccgcaagcc tcgaattact
     6001 ggcactattg ccgccacccg gaaggctatt atccctacgt gaagagttgt cccggcggtt
     6061 ggatgcaagt agttcctcaa taggtgttcc ctgagcaagg ctggcgggta gattcaggct
     6121 tctgcgtcaa gagattgctg aggggtggaa ggcttgcttc tgtccgattt caatattgtc
     6181 gatgctttta aaattggaaa aaagaaggta atgcagcaac cagggcgtgc agttgaagtg
     6241 ataagacgtt ttatcgataa catgactatg gaagggatat aaaacatgta caagggaatg
     6301 acagcattgc tggcggttat cgttctggta tacggtggcg cggcaatggc ccggggcaac
     6361 ggcgaccagc atggtagcgg acaattgggc ggagggggtc agcatggaaa ccagggcggc
     6421 atggcgtccg ggcatatgag cggtgagggg cgggacaaca caaacgcgca atggtcgggc
     6481 ggcgcaacta aaggccagga ccgttccgat ctcaagggcg gggatcacca gggacatggc
     6541 aagaaccaca gccaaaacca tggcaagggc aaaggccacg gccaaggtca tgggaagcat
     6601 caataagaaa gtaaattaac ggatgtaggc tgcgctaatc acaagatcag gcggaaaact
     6661 gaccggtaga ataaaaagca gttttgaagg gggagtggaa gtggcaactg aatcggttaa
     6721 ttattggaaa tttgcaacaa ttggaattct gttggtaggt gctaccgtcg ccgcgacgtt
     6781 gctcgttgcc gggcgggatt caaagactgg ctcatccgat gccgcgacgg tccagatgaa
     6841 cgaaccgccg atagatgcaa atacccagat tccacccggc aaagttgcgt cttcggagcc
     6901 tccgcgcaat gctcgggtta accagtctag taaagcctct tcgggcgctt tgcctcctcc
     6961 cccaccctat ccgcctcaag ccgcctatcc tccacaggtg gctcctcagc ctgcatatcc
     7021 cccccaatcg gtgatggatg ggtgcaaccg gtatgcccat gaaaaggtaa gcagcaaagc
     7081 cgaggaagtg ctcaagaacg cagctctcgg tgcggcaatt ggagccgcgg tcggggcagc
     7141 gggtggcgcc attgcgggag gaggaagcgg cgctggcaaa ggggctgcca taggcggtgt
     7201 tacaggtgcg gcgggcggaa cgctttacgg cctcaacgaa agcaaatcgc aagattcgca
     7261 ataccaggcg gcttacgcat cctgcatgcg ctcgaaaggc ttctgatcac cttatcctcg
     7321 ggccggggta atgccgaggg atatttttct tttttctttc ttctttcgta tcttctgccc
     7381 cctgccctcg ccatccaggc ccacccgctg gtaaccccca ctggtaatat tgagggtaag
     7441 gagggaagag tttattccat tatcgcaggc aactgcgcgg agagcgcggc tctttccctc
     7501 gttgctgaac ccattagcgc aaagccgagc agtttgccgt cggcactttt gaaaagggcc
     7561 ttcaagccgg tttcatcttc ctcgatgacc cattcccctt gcgcggtact ctccgggggc
     7621 gcgacgatgg tcggacacgc cggtgtcttg accagcaccg gcatagcagg atatttgacc
     7681 aaagtgtggt tttgggccag cgtggccgcg aggccgcgag ctgcatgcat gatgggcatg
     7741 acgaaaggaa gaactcttcc ttccacttct gcgcagtccc ccaaggcata aacacttgcg
     7801 tgatgagttt gcagggatcg gtttaccgca atgccgcggt ttacatggat gcctgccgct
     7861 ttcgctatcg tagtccgcgg acttagtccc accgcggaaa gcacgagatc tgtttctatg
     7921 gttttcccgc ttgcgagcgt caggcgaaaa ttctctccga tccgttcgac acgttcagtc
     7981 gcttcgccca ggtaaaatcg aacacccgct gcttccagct tacgctgcaa gaacgcccca
     8041 gctggtggcg ggagcagcct cccgaggggc tggttgctaa tatcgattat gcttgcctga
     8101 tagccagccg cgacaagatc attcgcgaat tcgcacccga tcagtccacc gcccaggatc
     8161 gttacgcgtt ttttgccttc gagcgcgtcg cgcaggcgct gataatcatc cagatcgttc
     8221 acggacagga tctcgtccgc agcgttacct tcaagcggca aacggacagg atcagccccc
     8281 agggccagga cgagttcccg gtaggagagc ctctctcccc cctctaaagc cactgctttg
     8341 ttgtgtgggt cgattgccgc aaggcgatga tgcgagcgaa cagttgcctt gagctgctcg
     8401 gccattttct cagcgctggc agttacgata gtggcgggtg tttttccggt cgccaaagca
     8461 tttgacagcg tcggcttgga gtaaaaaccg ccatgatctg aggaaacgat caggagcgga
     8521 gtttccgcat cgagcttgcg aaattcgcgc gcaacggtat aaccggctag gccgctccca
     8581 atgataataa tgggctcact catgatcgcc ttcggcttga tgatcgcctt gggcttgccc
     8641 gtccgaaacc ctcatgcgga cagcgaattg cggcgtggga gggctatctc ccggcttgac
     8701 gtgcttaaaa ctattccttg caaattttgt cactctggtt cctgctattt ctctcgggtt
     8761 tttatcaagt cggtattttg actgtttttg atttgcttaa gtggaatttt cagaagcttc
     8821 gatgcttgcc gcagctataa ggctcgaaat gggcgatttc tgggtagcgc tcttcattaa
     8881 agatcaaccc gataaaacat caccgctcgt gctcggtctt cttttagaga gtttttttgc
     8941 aatgatgggt aaatttcgga tataaatagg aaattttatt tcactgccat gactgagcgg
     9001 ccgaggggtc agtcaagccc tgagagtatc aagcagtggt ccatgtactg cgctggcttt
     9061 tcaacaaatt atcggacagg tttcaccgcg acctctgtgc ctggggaaga cctcggcgtg
     9121 caggccagaa aattcttaag caagcaggtt ttttaatcaa ggtacacggc ggagatggcg
     9181 aatatcggct tcattggtct cggtatcatg ggcaagccta tgacaaggcg cctgatcaaa
     9241 ggtggtcata ccttgttttt gtactccaga agcggcgtgc cggaagaatt gctcaagcag
     9301 ggaggaaacg cgtgcccttc gcctgtagaa gtcgccgaaa attctgacgt catcatcacc
     9361 atgttgcccg aggcaaaaga cgtggagcaa gtgctttttg gagatagagg gatagcttgc
     9421 ggccttggca gtgccttgcc gaacgccgat ctgcatgaac ctaaaacaat cgtcgacatg
     9481 agttcgatct ctcctatcga aaccaggcac tttgcggaaa gaatcggccg gctcggctgc
     9541 cattatatgg acgcgccggt atcgggcggc gatatcggtg caaggaatgg cacgcttacc
     9601 atcatggtcg gagcttcgga agaggtgttc cagagagtac tacctgttct cgaactgatg
     9661 ggtcagacta ttactcgtat tggaaacata ggttcgggac agatttgcaa gatatccaac
     9721 cagattatcg ttgcattgac tattgaggca gtgggagaag gtttgctgtt tgcgtcaaag
     9781 gcaggtgcag acccggtcaa ggttcgtgaa gccctgctcg gtggctttgc tgcctcgcgc
     9841 gttcttgacg ttcatggcgg gcgcatgatc agaaatgcgt ttgaacccgg atttcgggtc
     9901 gaactgcatc aaaaggatct tggtatcgca ctttcgaatg ctcgtgcaat gggtatcagc
     9961 ttgcccaata ctgcttctgt tcaggaaatg ttcaacgcct gtgtcgccca cggcgattca
    10021 aaattggaca gctccgccgt agtcaaggtg cttgaaaagc tggcgaacca cgaaatccag
    10081 ccaggccagt tatgagttct cttgatctgg cagccaggct acgcgatttt ctgccccccg
    10141 aagcggtgct tcaggaagtt gaggatctta agccatacga gtgcgacggt ctgtccgcat
    10201 accggcggat accattgcta gtggcgttgc ccagtaccga agaagaggtt gctcgcgtgt
    10261 tgcgcgtttg tcattcgttg aaagttcccg tggttgcccg tggagcggga accggactgt
    10321 cgggaggcgc actgccccac gcagagggcg ttcttctttc gcttgcgaaa ctgaaacgca
    10381 tactggagat tgacccgaag gcacggactg cgcgggtgca gccaggggtg cgcaacctcg
    10441 ccatcagcga ggcagcggcg ccctatggct tatattatgc tcccgatcct tcttcccaga
    10501 ttgcatgttc cattggcgga aatgtggcgg aaaactcggg tggcgtgcac tgcctgaaat
    10561 atgggcttac cgtgcacaac attcttaggc tgagagtact gacggcagag ggtgagttgc
    10621 ttgaaatcgg gagcgagggc ctggattcac ccggctacga ccttctcgcc ctcatgacag
    10681 gcagcgaagg catgcttggc attgtgacgg agataatcgt aaagctcatg cctcgtcccg
    10741 aaaaagccca agttatcatg gcagcctttg acgatatacg caaggcgggg gacgcagtcg
    10801 ctgacgtcat tgccgctgga gtcattccgg cggggatgga aatgatggac aggataacta
    10861 ctcgggcggt ggaggagtat ctcaatgcgg gatacaacct gagtgcagcc gccattcttc
    10921 tgtgcgaatc ggacggcacg gcggaggagg ttgcggagga gattcagcaa atcgacgaga
    10981 ttatgaaccg gaacggggcg acggagatca aagcatcttg caatgaaccg gagcggttga
    11041 ggttttgggc aggccgtaaa gctgccttcc ccgcggcagg ccgcatttcg ccggactatt
    11101 actgcatgga tggcaccatt ccgcgtaaga agttggctca agtgttggcc ggtatcgagg
    11161 ccttgtctgc ggaatatgga ctgcgatgca tgaacgtttt tcacgcaggg gatggaaatc
    11221 tgcatccgct cattctttat gacgccaata atcctggaga actggccagg gcggaggaat
    11281 ttggcgcgag gatattgcag atgtgcatcg atgccggcgg aacgataacg ggcgagcatg
    11341 gggtagggat cgaaaaaatt gatcagatgt gctctcaatt caagagtcct gagctggaga
    11401 tgttccatgc ggtgaaagcg gcttttgacc cagacgggct gctgaatccc gggaaagcgg
    11461 tgccgacgct gcgccgctgc gccgagcacg gcgccatgca cgtgcatcga ggtgaggaaa
    11521 aattttcaga acttccgcgg ttttagcggc ttatccgatt gcccataatt tcctatcctc
    11581 tctaatctcc tggattggct gatgcaggac atgatcgatc aattttccga tacgattcgc
    11641 gtggctgtgt tgcggcgcac cgcactgcgt attcgcggag gagggaccaa ggatttttat
    11701 ggcaacagca taacagccgt gggggatgcg tcctcggaaa acgagatcct cgatccatcg
    11761 tcttttgcag gcctcgtgga ctacgagccg acagagcttg ttgtgactgc acgcagcggc
    11821 acgcgactgg cagacctgga aggggcgctt catgagcatg gacagatgct cccttttgag
    11881 ccgccccatt ttggatccga agcaacactc ggcggatgtg ttggcgccgg tctttccgga
    11941 ccccggcgcg cctccgcagg ggctgtgcgg gactttgtac tcggtgttcg catgctggac
    12001 ggcaaggcgg acgacatgca atttggcggc agagtcatga aaaacgttgc aggttatgac
    12061 atatcccggc tgttgaccgg ttcaatgggt acactgggtc tgttgctgga aatctctctc
    12121 aaggtactgc cccttccgcc acgagagctt actctgtact ttcggatgag cgagtctgaa
    12181 gcgatcaaga agatgaatga atgggcgggg agaccacttt cgatttctgc cacctgtttc
    12241 actgatggtg aactggcggt gaggctgtcg ggtgccgagc ctgccgtacg cgcggccagg
    12301 caaaagctcg ggggagaggc aatcgctgaa ggcggggcat tctggtcggc tgtccgcgaa
    12361 cagacgcatc cattttttca acggggcgga caactctggc gcttgtcgat caaatcgact
    12421 gctccacttc tgtcattacc cggcaaacaa ttgatcgagt ggggcggtgc attgcggtgg
    12481 ctgatgctcg atgaggaccc cgatcaggaa tcggtgcatt cagcggtacg ccaggcagcg
    12541 gaaagcgcag gtggccacgc tacattgttc agaagccctg gaccgcgtag caatgtattt
    12601 catccgcttt cccccgcgat ggcccggatc acccagagcc tcaaggaaaa gttcgatccg
    12661 caccgcatat tcaatcctgg ccgtatgtat ccctaaattt aagatgcaga ccaatctcgc
    12721 tgatttcata aaggacacgc cggaaggcca ggaagccgag gagatcctcc gttcctgcgt
    12781 ccattgcggc ttctgccttg ccgcctgccc gacctaccag ttgcttgggg acgagctcga
    12841 tagcccccgc ggccggatat atttgatgaa gcaggtattg gaaggcgcgc ctgttactga
    12901 aaaaactcaa ttgcatctgg accgttgcct gacctgcagg gcttgcgaaa cagtttgtcc
    12961 atcgggggta cgctatgggc gcttggtgga tattggccgg gatattgtcg aaaagaaagt
    13021 gggacgggat cgttcctctg gcgccgtcag gtacgcactg cgtcaggtat tgcccaaacc
    13081 aggggttttc aggacattgt tcaaactcgg gcaaacagcg cgtccactgc ttggactgct
    13141 gtcggtacag cttcgcgagt ccattcccca atcaaaaggg acgcaatcca cctctttctg
    13201 gcctccccct cgacatccgc gaaagatgct ggtactcgac ggttgcgtcc aaccgacgct
    13261 tgctcccaat atcaatgctg cggccgcacg cgtgctggat cgcctcggcg tctcgctgat
    13321 caaagcctcg gatgcgggct gttgtggtgg gttatcgtat cacctcaatg cgcagcagga
    13381 aggtctggat tatatgcggc gcaatatcga tgcctggtgg ccatacatcg ccggccagtc
    13441 gagtggggcg agaggcccgg gggagggtgt cgaggcgatc gtcataactg ccagcggttg
    13501 cggagttacg gtaaaggatt acgggcattt gctgaggcat gatgccgaat acgcgggaag
    13561 ggcagcgcac gtatccgcca tggcgaagga tatcagcgag gttatcgatg ccgagaccga
    13621 aacgctgttg ccccttttga aggaggggac gctccttgag gatgaaaagc aaaagcttgc
    13681 tttccattcc ccctgcaccc tgcaacatgg aatgaaaatt cgcggggttg tggaaaggat
    13741 tttgcgggaa gcaggattcg aactcactta cgttcctgat gcccacctct gctgcggatc
    13801 ggcgggaacc tactccatac tacagccgga attatcgaaa cagctattgg cgaacaaggt
    13861 cgcagcgctc gaatgcggtg cccctgcccg cattgcaacg gccaacatcg gctgcctcaa
    13921 gcacttgcaa agtggaaccc ggtttcccgt tgatcactgg atagagatat tggacgaaag
    13981 actgcagcac gacgacgaga aagcacagat tgccgcgggg ccgattccag cctaggcctt
    14041 ttgactcttc gccagaatgc ttgctttgga cgatgactgc gcgcgggaca tgcttttgac
    14101 gattattgtt gcaatcccgt accgttatcg ttaaaccctg caacatccaa ttatcatgac
    14161 cctgtcaacc gatcagaata ttgaccctga gagactttct ccggctgcga gaagaatgct
    14221 cgagcttcga gacgaggtat ttgcggaatg gataaaaaga gttcgacaaa ccgtcaagga
    14281 agctgaacaa ctctctcaac caattttgat caataccttc ccatccctat atgaaaacct
    14341 tgcagaagcc attacccctg attaccctag agccacagga gatgaaggta ataccgtggc
    14401 taccgaacat gggggcgaac gggccaggct gacgaattac aatgcacgtt cggtcattgc
    14461 ggaatatcag ctgctgagat ggacaacatt tgatgttctc aagctgaatg acgtgcagct
    14521 taacgatgac gaagtttttg tgatcaatgc gtccattgac ggatcgatac gagaggcagt
    14581 taccgcattc gctcttgcgc aaactgtgtt gcgggaaaga ttcgctgcga ctcttgcgca
    14641 tgaccttaga aatccgttat cagcggcaca cgtgcttgcc cagctaatcc aacgttcttc
    14701 ggatctgggt aagataaagg agttcgcggg acaaatcacc gacaatctgg ggcgggtgga
    14761 tcgtatgatc caggatttac tcgatgccgt cacgttccaa accggagaac ggctgcccct
    14821 tcgcctcgag gaattcgacg tacaagaggt ggcgaaggaa gtatgcgatc agttcgagag
    14881 tgtgcatgga cctcgctttc gatttattgg caagacggta ataggttggt gggatcggga
    14941 cgccattcga agggcactgg aaaatatgat tggcaacgcg gtgaaatacg gagcgcctgg
    15001 cacccctata cgaatcaaga tagatcccat agatgagcgc atgttactct ctgtgcataa
    15061 tgaaggagaa cctattccac ccgagcaaat cgagtgcgtg ttccaggtat tccgaagggc
    15121 agttgcggca aaagaggaca aggaaggctg gggtataggc ttgccatacg ttcgaagtgt
    15181 cgcggagagc catggtggaa gtgttggagt cgatagctcg gcagagcggg gaacaacctt
    15241 tctcattgac attccagtcg acgcacggcc ttttcaggat gcccccacgc ttggcagaaa
    15301 accggactga tgcaggaaca gaagaggtga ttgaaagccg attcgtgtgg cgtttctgtt
    15361 tttgggtaat catgcatcca gtacttcgta tcaaatgatg cagagaaatc gccatctcaa
    15421 taattttctc cttccctgtc gtttcgaata aacctaccaa ggaacctcaa gccagcgcgt
    15481 cacgcgccaa ggtctctccc ccatttcgat tcatcggccc ttgcccagct gatctgacct
    15541 tttagagctt ttgcattaaa cagcaaaaca cttcggtagt ttgtgctcga aaccagcacg
    15601 ccgatgattg ccaactttgc gaagatggtt ttgagccttc cggtctataa tctcacctat
    15661 cctctcttta aggctttcaa gtacatgagc ttcaccagca agccgatggc aaagaagacc
    15721 agttccagcc ccttacctcc ccggttggca aggttgctgc gtgaatctgg tggaattgct
    15781 ttgcttgggg tcatgctcta tctggcgttg atattgtatg gctacgatcg cgtggatccc
    15841 ggctggtctc atacagggga gggagcactc cagaatcccg gggggacggc aggagcctgg
    15901 cttgcggatc tgttgctcta cctgtttggc gcctcggcct ggtggtgcat actcttcctt
    15961 gcttatttga tatcacttat cttccgcgga ggcgaaagta atggggtttt cgatcggcgg
    16021 cccttgcttg tctcggccat cggcttcctt gtactgctgc tggcaagcag tggacttgag
    16081 tccttgcggt tcgggacgca caagctgcca ttgccgcagg ctcccggggg cgtcctgggt
    16141 ctggtcatca gcaccaatct gtctcaagca ctcgggttca tcggggcgac tctcgcgctc
    16201 ctgatccttg tcgccattgg attcagcctg ttcaccggcc tgtcatggtt gcgctttgtc
    16261 gaaaaactgg gcgctatgct ggaaggcgcg tgcgtgtcag ccaaagagcg ttgggaagcc
    16321 tggcaagacc gtcgcgccgg cgcgatctcg gcagtcaaac gtgacgcgtt ggtcgaggtc
    16381 gggaagaagc gtttgcatga gcatcctcct ctgcatatcg agcaaccggc gagcctgatc
    16441 cccaaatctc cgcgagtact gaaggagaaa caggccccac tcttcctcga ccttcccgat
    16501 tcacccttgc cgccgctgca tcttctcgat caaccggcgc ataaggagat ggaagttcta
    16561 tctcccgaga cactggaatt cacttcgaga ctgatcgaac ggaaactgat ggattttggg
    16621 gtggaagtta aagtcgtaac ggcattccct ggtccggtca ttacccggta cgaaatcctg
    16681 cccgctgtcg gggtcaaggg taaccagatt ctcaatcttg taaaagatct cgctcgatcg
    16741 ctgtcggtcg tgagcatccg tgtggtcgaa accattcccg gaaagaccag tatggggctc
    16801 gaaattccga atcccaagcg gcaggtggtg cgattgaccg aaattctcag ctcccaagcc
    16861 tatgctgaca tggggtcgcc tcttacgatc gcgctcggga aggatattgg cggccatccg
    16921 gttgttgctg accttgccag gatgcctcac gtactggtcg ccgggacgac cggctcgggg
    16981 aaatcggtcg cgatcaacgc catgatcctg agcctcttat acaaggcaac acctgagcag
    17041 gtccgcctta tcctggtgga tccaaaaatg ctcgagttgt cagt
//
LOCUS       DASSHJ010000186         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1425482,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000186 DASSHJ010000000
VERSION     DASSHJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425482"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1195
                     /locus_tag="VFI43_07230"
     CDS             410..1195
                     /locus_tag="VFI43_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061954.1"
                     /translation="MLRHFPQPDIAYPHRVRDYFIAALTFVCVAISLNIDANGTLEQQ
                     NRMGLIAWTFLAALLLGENKEIRMQVIVAVAFATLGEHFASIYMQGYTYRFGNVPAYV
                     PPGHGMVYLTAVALARSGFFLRYSRKIATFVVLTCGAWSLWGISGYPEQGDQVGALLF
                     CIFLIYLFRGRSPLVYLGAFFITTWLELIGTAAGTWKWASIEPVLSLSQGNPPSGVAA
                     WYCLVDAVAIGSAPLLLRMLAKGNEWFRVGRAQKDAHQGARNE"
     gene            1436..2920
                     /locus_tag="VFI43_07235"
     CDS             1436..2920
                     /locus_tag="VFI43_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7061955.1"
                     /translation="MSFTQLNLSNEILRAIADQGYTEPTPIQTQAIPPILDHRDIMGA
                     AQTGTGKTAGFTLPMLHLLQQHANSSTSPARHPVRALILVPTRELAMQVYESVQAYGK
                     YIPLKYTAVFGGVDMDPQIRELRAGTEILVATPGRLLDHVQQKTVNLSRIEILILDEA
                     DRMLDMGFLPDIKRILSLLPENRQSLLFSATFSEEIKKLANKLLKDPISIEVAKRNSI
                     TELITHVVHLVPHSQKRELLAHLVKSENLKQVLVFVRTKHGASRLAQQLEREGIAATA
                     IHGDKTQAQRTEALARFKQGAVRVLVATDVAARGLDIEDLPHVFNYELPASPEDYIHR
                     IGRTGRAGMKGNAVSLVSEDEAELLENIEKLLKLKLIKKTVAGFELVAPGQKPLEHTN
                     RHVGHEGRDAEQRKRAGVADSRKNGSGKKERRPVPAGRSKIAEDPLFSQPYTPSVGKN
                     QSDPGGTSPAYEHPDHLRYGHKEQAIAALFMPRPPRKPQQDGEA"
     gene            2966..3529
                     /locus_tag="VFI43_07240"
     CDS             2966..3529
                     /locus_tag="VFI43_07240"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7061956.1"
                     /translation="MGKRLTKIYTRTGDNGTTGLGDGSRVLKTSVRIEAIGEVDELNS
                     FIGLLLAEELDEDVRLRLLNIQHELFDLGGDLSIPGRVSMQSTQVVRLEKELDELNAT
                     LDPLREFILPGGLRAAALCHIARSIARRAERRLVSLYQDEPVIPIHIHYLNRLSDLLF
                     VLSRILNRCSEAGETLWQPVKGTDPAV"
     gene            complement(3545..4078)
                     /gene="lspA"
                     /locus_tag="VFI43_07245"
     CDS             complement(3545..4078)
                     /gene="lspA"
                     /locus_tag="VFI43_07245"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7061957.1"
                     /translation="MKLSVSLIIASVVVVLDLATKRWVESELFHGQQIPLTSFFNLVL
                     TYNAGAAFSFLSDAAGWQRWFFSLVAVGASVMIVYLLRKHAHDTLFCVALSLILGGAL
                     GNLWDRISLGHVVDFLDFYLGPYHWPAFNVADSAIFVGAVLLIVESFRGSETGTGKTS
                     AIEDENNGKGRAEKRRS"
     gene            complement(4109..>4722)
                     /locus_tag="VFI43_07250"
     CDS             complement(4109..>4722)
                     /locus_tag="VFI43_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7061958.1"
                     /translation="GGFYLDILKDRLYTAAAKGASRRSAQSALYHIAQSLVRLFAPIL
                     SFTSEEAWEYLTGDREGSVFLHTWHAFPEQLGGEDLIWRWTRIRELRSRVQKALEEAR
                     AEGRIGSSLAAAVEIYMQGEDFALLDSLKDDLRLVLITSQAQAILALGSTDERVVVTP
                     SGYPKCERCWHYRADVGIDPEHPRICGRCVSNLYGSGEIRHYA"
BASE COUNT         1134 a         1309 c         1204 g         1075 t
ORIGIN      
        1 cctgcatcga ttgccaccag aacctggtgc acaaggaagt ggccgagacc gacctgaacg
       61 aaagcaagaa ggcaggcaag atggtcctca agcccgaaaa gaaatccaag gatgaggacg
      121 aggacgacga ggaagagtaa attcgtgtcg aatcgtaacc tgtcatcctg ttgatctggc
      181 cccttttgat cgagtcgttc ctgtgttggc cgctgtttca tttacgaatg gaacagcggc
      241 ttcatttgtc ggtgtctccc cccggtctct tcgcctgaac gacaggaatc cgaccgtcgt
      301 ctcactgcta cgacttgatt taaccctatt tcaatacgat ttcgaaatcc gttgcatgca
      361 ggagacggat tttgcatctg accccatact ccctgccgga tgctctgaaa tgctgcgtca
      421 ttttccccaa ccggacatcg cttatcctca tcgggtacgc gattatttta ttgcggcttt
      481 aacctttgtt tgcgtcgcca tcagcttgaa catcgacgcg aatggcacgc tcgagcagca
      541 gaatcgaatg ggcctcatcg catggacctt cctcgccgcg ttgctgttag gtgaaaacaa
      601 ggaaatccgg atgcaggtta tcgtcgctgt cgcgtttgcc acgctcggcg agcattttgc
      661 atccatctac atgcagggtt atacctaccg cttcggaaat gtccccgcat acgtgccgcc
      721 gggccacgga atggtttatt taactgcggt tgcgctggca cgatcaggtt ttttcctcag
      781 gtattcacga aaaatagcca ctttcgttgt cctgacgtgt ggagcgtggt cactttgggg
      841 aataagcggt tatcctgaac aaggcgacca agtcggcgcc ttattattct gcatatttct
      901 catttatctt ttcaggggtc gctcaccgct cgtgtatctg ggggcattct ttattacaac
      961 ttggctggag ttgattggaa ccgccgccgg aacgtggaaa tgggctagta tcgagcctgt
     1021 tctctcgtta tcgcaaggta atccacccag tggagtcgcg gcatggtatt gtctggtcga
     1081 tgctgttgca atcggatcag cgcctctgct cctgcgcatg ctcgcaaaag gtaacgagtg
     1141 gttccgggtg ggaagagcgc aaaaagatgc tcatcaaggt gcacgaaatg aataaagcac
     1201 tgtctatatt gcgtttataa cggtttttca ggttgctgag aatcctctcc cacgtatcca
     1261 atggcacatt ttggattccg catcctctca ttgtcgcatt tcatcatact tgtcgctctc
     1321 attttacaaa cccctgccct ggctccttcc gaagcgcgcg gccttgattt ggagacctgc
     1381 cggcctcatg ctggaggtct actgacgttc gtagtggaaa ccaggcagca actatatgtc
     1441 ctttactcaa cttaatttat ccaatgaaat tctgcgagcc atcgcagatc aaggttacac
     1501 cgagccaact cccattcaga ctcaagcaat tccacctatc ctggatcaca gggatatcat
     1561 gggagcggct cagaccggta cggggaaaac ggccgggttc acgctgccga tgctccatct
     1621 gcttcaacag cacgccaact ccagcacatc acccgcaaga catcccgttc gcgcattgat
     1681 ccttgtgccc acacgtgaac ttgcgatgca ggtttatgag agcgtgcaag catacggtaa
     1741 atatattcct cttaagtata ccgcggtctt cgggggagtc gatatggatc cccagatcag
     1801 ggaactgcgc gcagggaccg agattcttgt ggcaactcct ggacgtttgc tggatcacgt
     1861 tcagcagaaa accgtcaatc tttccagaat cgagattctg attctggatg aggcagatcg
     1921 catgctcgac atgggatttc tcccggatat caagcgtatt ctttcgttac tgccggagaa
     1981 tcgacaaagc ttgctgttct cggccacatt ttctgaagag atcaagaagc tcgcaaacaa
     2041 gctcctgaaa gaccccatat ccatcgaggt cgcgaaacgt aactccatta ccgagcttat
     2101 cacccatgtg gtgcatctcg ttccccacag tcaaaaacga gagctgttgg cgcatctcgt
     2161 caaaagcgag aacctcaaac aggtgctggt gtttgtacgc accaagcatg gagcaagccg
     2221 actcgcgcag caactggagc gggaggggat agcagcgaca gcgattcatg gagacaagac
     2281 gcaggcccag cgtacagagg cactggcgcg gttcaagcag ggcgccgtaa gggtgctcgt
     2341 agcaacggat gtcgctgcca gaggcctcga tatcgaagat cttcctcatg ttttcaacta
     2401 cgagttgccc gcatctccgg aggattatat ccatcgcatc ggtcggacag gtcgcgcggg
     2461 gatgaaagga aatgctgttt cactggtaag cgaggatgaa gcagaactgc tggagaacat
     2521 cgaaaagctt ctcaaattaa agcttatcaa aaaaaccgtt gctgggttcg agctggtagc
     2581 gccggggcaa aaaccacttg agcatacgaa ccgccatgtt gggcacgagg ggcgagacgc
     2641 tgaacagaga aaacgcgcag gtgtggcaga cagccgcaag aacggcagtg gcaaaaagga
     2701 acgtcggccc gttcctgcag gccgttcaaa gattgcggaa gaccccctgt tcagccaacc
     2761 ctatacgcct tccgtcggca agaatcagtc cgatcctggc ggtacttccc ctgcctacga
     2821 acatccggat caccttcggt acggacacaa ggagcaggca attgccgcgc tttttatgcc
     2881 tcggccccca cgcaagcctc aacaggatgg cgaagcgtag cctccctctc taagcgttct
     2941 cgcatccttc agcaggagtt cctgggtggg taagcgcctg acaaaaattt acacacgtac
     3001 cggagacaat gggactacgg gcttgggaga tggtagtcgc gtcctcaaaa cctcagtccg
     3061 cattgaggca attggcgagg tggacgagct caacagcttt attggcttac tgcttgctga
     3121 agagctcgac gaagacgtcc gtcttcgcct gttaaatatc cagcacgagc tttttgacct
     3181 gggtggggac ctaagcattc ccggacgagt gagcatgcaa tccacgcaag tcgttcggct
     3241 tgaaaaagaa ctcgacgaac tcaacgcaac gctcgatccg ttgagagagt ttattctacc
     3301 tggaggtttg cgggcggctg cgttatgtca catcgcccga tccatagcac gacgggcgga
     3361 aagacgcctg gtaagcctat atcaggacga accggttata cccatccata ttcattatct
     3421 caaccggcta tcggacttgt tgttcgtcct gagcaggatc cttaatcgtt gcagcgaagc
     3481 cggggagacc ctatggcagc ctgtaaaagg cactgatcct gccgtctgaa accctcattc
     3541 aagtttaact tcttcttttc tcggctctac ctttaccatt attttcatcc tcgatcgcgc
     3601 tggtctttcc ggtgccagtt tcgctgccgc ggaaactctc aacgatcagc aacacggcgc
     3661 cgacaaaaat tgcggaatct gccacattga acgccggcca gtgataaggg ccaaggtaga
     3721 aatctagaaa atccacgaca tggccgagcg agattcgatc ccaaagattc cccagcgcgc
     3781 cgcccagtat caggctcagc gcgacacaga atagcgtgtc gtgcgcatgc ttgcgcagga
     3841 ggtaaacgat catcaccgag gcccccactg caacgagact gaaaaaccag cgttgccatc
     3901 ctgccgcatc gctcagaaag ctaaacgccg cgcctgcgtt ataggtcaat acgagattaa
     3961 aaaagctcgt caggggtatc tgttgcccat ggaataattc cgactccacc cagcgttttg
     4021 tagccaaatc caggacaacc acaacactgg caatgatcag gctgactgat aacttcatct
     4081 ttaccgccag aacgggggac ttcctgcctc aggcataatg tcggatttcg ccggaaccgt
     4141 aaaggttcga gacacatcgg ccgcaaatgc ggggatgctc aggatctatt cccacatccg
     4201 ctcgatagtg ccagcaacgc tcgcatttcg ggtatccgct gggagtcacg actaccctct
     4261 catccgtgct acccaaagcc aggattgcct gcgcttgcga agtgatgagc accaggcgaa
     4321 gatcgtcttt caaggaatcg agcaacgcga agtcctcccc ctgcatataa atttccaccg
     4381 ccgccgcgag cgatgagccg atcctcccct cggcgcgcgc ttcttccagc gccttttgca
     4441 cccgggagcg aagttcgcga attcgtgtcc agcgccatat caaatcctct ccacccaatt
     4501 gctcagggaa ggcatgccaa gtgtgcagga aaacgctccc ttcgcgatct ccggtgagat
     4561 actcccaggc ctcctctgag gtaaaactga gaataggcgc aaacaatcga accaggcttt
     4621 gagcgatatg atatagcgca gattgtgcag agcgacgcga ggctcccttt gctgctgccg
     4681 tatacagccg gtctttcagt atatccagat agaagccgcc ca
//
LOCUS       DASSHJ010000187         8027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_688370, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000187 DASSHJ010000000
VERSION     DASSHJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688370"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="mnmG"
                     /locus_tag="VFI43_07255"
     CDS             complement(<1..1269)
                     /gene="mnmG"
                     /locus_tag="VFI43_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7061959.1"
                     /translation="MHSPERFDVIVVGGGHAGTEAALAAARMGCRTLLLTQNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAAADEAGIQFRILNSSKGPAVRATRAQADRA
                     LYRQAIRSRLENEPNLSIFQQSVDDLTLEGERVVGVVTQLGIRFFARAVVLTAGTFLG
                     GRAHVGTANFAAGRAGDPPSTSLAQRLRELKLPVGRLKTGTPPRLDGRTIDYSVMVEQ
                     PGDNPVPFFSFLGNAAQHPRQLPCWITSTNSRTHDIIRGGLDRSPLFTGIIEGVGPRY
                     CPSIEDKVVRFSARESHQIFLEPEGLQTCEVYPNGISTSLPYDVQVELVRSIRGLEDA
                     YITRPGYAIEYDYFDPRALKQSLENKFIEGLFFAGQINGTTGYEEAAAQGLLAGLNAG
                     LKVQEREAWCPRRDEAYLGVLVDDLTTRGVT"
     gene            complement(1456..2817)
                     /gene="mnmE"
                     /locus_tag="VFI43_07260"
     CDS             complement(1456..2817)
                     /gene="mnmE"
                     /locus_tag="VFI43_07260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7061960.1"
                     /translation="MPAPDIIAAVSTPPGRGGIGVVRVSGRDLRLLAEKVVGSMPKPR
                     YATLSHFLDEDGQVIDQGIALYFPAPHSYTGEDVLEFQGHGGPIVMSLLLASCISAGA
                     RLAEPGEFTLRAFFNDKLDLAQAESVADLIDAGTREAARSALRSLNGEFSSLIHEIVS
                     GLTDLRMIVEAWLDFPEEDIDVLAQPHIDERLGDIRGRLDEVFLSARRGSLLREGVSV
                     VLVGKPNVGKSSLLNQLTGEEAAIVTEVPGTTRDTIERPIEIKGVPMHVVDTAGLRET
                     EDLIESIGIERTRSAIEKAALALVVLDCREGLAQEDRLIIESLPANLQRVYVYNKIDL
                     MDHALPAWNGPTSSGETEIYVSAKTGAGMEALRDAVLDLAGWNLESGGGVFMARQRHL
                     VALSEATGHLTHADDLAGSGKPLELLAEELRLAQIALSSITGEFTADDLLGEIFSRFC
                     IGK"
     gene            complement(3024..4712)
                     /gene="yidC"
                     /locus_tag="VFI43_07265"
     CDS             complement(3024..4712)
                     /gene="yidC"
                     /locus_tag="VFI43_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7061961.1"
                     /translation="MDTQKLVLFLIFSTSMLFLWEAWQKEQYAALPKPAVEGSSSQSG
                     EHVAPIPGEKLSSTQAGTSTPGAENITPGRPSSILESGEKIVVKTDVVTAEIDTAGGD
                     LRRLELLNYPDRDDKNKSFALLESQGDQLYVTQSGLIGEGLPTHKTQYTAEAGTYSLE
                     PGKDKVEIRLSAPEADGVQVTKVYTFHRGKYLIDVSFEVSNHRAAPIQPFSYFQIVRD
                     SKPPVGSIFMIPTYTGAAVYTEQDKFKKIEFSALDKGNASYPKNADNGWIAMLQHYFM
                     TAWLPKDKQPREYYAKRMAENQYSAGVIVPVGLIEPGKTAVVDVPLYAGPEEQSKLAS
                     IAPGLDLTVDYGWLTVIAAPLFWLLSFFHSWTDNWGVAIILLTMSVKAAFFPLSAAGY
                     RSMAKLRLVTPKLQRLREQHGNDRQRMHEAMMQFYKTEKINPMGGCLPIVVQIPVFIS
                     LYWVILSSVELRNAPFALWIQDLSVPDPYYVLPVIMGVSMFIQSKLSPPATDPIQQKV
                     MQIMPFAFSVFFFFFPAGLVLYSLVNNILSIAQQWQITRMIEGAAATTALSKKR"
     gene            complement(4765..4974)
                     /gene="yidD"
                     /locus_tag="VFI43_07270"
     CDS             complement(4765..4974)
                     /gene="yidD"
                     /locus_tag="VFI43_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7061962.1"
                     /translation="MSQIIIGFIKLYQYCLSPFLGPSCRFSPSCSHYACEALARHGAM
                     RGSCLSIWRIMRCHPWSQGGDDPVP"
     gene            complement(4956..5282)
                     /gene="rnpA"
                     /locus_tag="VFI43_07275"
     CDS             complement(4956..5282)
                     /gene="rnpA"
                     /locus_tag="VFI43_07275"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7061963.1"
                     /translation="MRKPEEFSSVLRFRCSANSELLQVFAKPNGLSQSRLGLIVAGKI
                     ERLSVRRNRTKRLLREAFRARQHELGGLDLVVRLRSRTMQDDSPRIRAEADELMVQLQ
                     RCRKLS"
     gene            complement(5336..5473)
                     /gene="rpmH"
                     /locus_tag="VFI43_07280"
     CDS             complement(5336..5473)
                     /gene="rpmH"
                     /locus_tag="VFI43_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7061964.1"
                     /translation="MKRTYQPSVTRRKRTHGFRVRMKTTGGAAVIRARRAKGRVRLAV
                     A"
     gene            5657..7060
                     /gene="dnaA"
                     /locus_tag="VFI43_07285"
     CDS             5657..7060
                     /gene="dnaA"
                     /locus_tag="VFI43_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7061965.1"
                     /translation="MPAMDNFWRSCVGYFEKELSAQQFNTWIKPLRLQPATGETSDPI
                     LIAPNRFVLQWIKDNFLAHIERMAEQYFCRTVRFQLALSDPLSNHVAASPAPVNTIAP
                     APSSRADLDAPAVVIKKPNGDAREKNASRLNASFTFENFVTGRANQLARAGAIQVAER
                     PGVAYNPFFIYGGVGLGKTHLIQAIGNLVLEHNRSAKVRYIHAEQYVSDVVKAYQHKA
                     FDDFKRYYHSLDLLLIDDIQFFGGKNRTQEEFFYAFNALIEAHKQVIITCDSYPKEIS
                     GMEERLISRFGWGLTVAVEPPELEMRVAILLKKALMENIMLDENVAFFIAKHIRSNVR
                     ELEGALKRVVAYARFTGHALSLDLAREALKDLLAVQNRQISIENIQKTVADYYKIKVA
                     EMYSKKRSRVVARPRQMAMAISKELTPLSLPDIGEAFGGRDHTTVLHGYRKIAELRAS
                     DPAINRDFNTLLHILRG"
     gene            7261..>8027
                     /gene="dnaN"
                     /locus_tag="VFI43_07290"
     CDS             7261..>8027
                     /gene="dnaN"
                     /locus_tag="VFI43_07290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7061966.1"
                     /translation="MKLIQTERDILLKPLQVVTGIVERRHTLPILSNVLIERKDDRIS
                     FVATDLEIQITTSAQDGATASEDHGVTVAAKKLQDILRALPDKTQVALETEGSRLHVK
                     AGKSRFHLQTLPVEDFPKFPAGSEPQEKITLKQKELKHLLSMVQYAMAQQDIRYYLNG
                     LLLLTEDHQLKVVATDGHRLAFAVMPLEAGEENPEARREVILPRKVVLELTRLLNDTD
                     EPVTVEMLQNQVRFTFGAVVLVTKVVDGKFPDYNRVIP"
BASE COUNT         1903 a         2319 c         2083 g         1722 t
ORIGIN      
        1 agtgacccct cgggtagtga gatcatcgac gagtacaccg aggtaggctt cgtcgcgccg
       61 aggacaccat gcttcccgct cctgcacctt gagccccgcg ttgaggcccg ctagaagccc
      121 ttgtgcagcc gcttcctcgt acccggttgt cccattgatc tgtccggcaa aaaacaaacc
      181 ctcgatgaat ttgttctcca gagattgttt cagcgcccgg ggatcgaaat aatcatattc
      241 gatcgcgtag ccgggacggg taatatacgc gtcttccaat ccgcggatcg atcgtaccag
      301 ctccacctgc acatcatagg gcaaactggt tgaaatcccg ttcggataga cctcgcaggt
      361 ttgcaggcct tccggttcaa gaaaaatctg atgcgattcc ctcgcggaaa atcgcaccac
      421 cttatcttct atcgaagggc aatatcgagg gccgacgcct tcgattattc ccgtaaacaa
      481 gggcgaacga tcaaggccgc cgcggatgat atcgtgggtg cggctgttgg tggaggtgat
      541 ccagcacggc aattgccgag ggtgttgagc ggcgttaccc aggaaggaaa agaaaggcac
      601 cggattgtcc ccgggctgtt caaccattac cgaataatca atcgttcggc cgtccagccg
      661 ggggggtgtc ccagttttca aacgcccgac cggcagcttc agctctcgca agcgctgcgc
      721 caggctggtg gaagggggat cgcccgcgcg gccggcggcg aaattcgccg ttcctacatg
      781 tgctcgcccc cccagaaatg ttcccgcggt cagcacgaca gcgcgcgcga aaaagcgtat
      841 gcccaattgc gtaacgacgc cgaccacgcg ttcaccctca agcgtcaggt catccaccga
      901 ctgttgaaat atcgaaagat tcggctcgtt ctccaggcgg ctgcgaatcg cctgacgata
      961 aagagcgcga tcagcctggg cgcgcgttgc ccgaacggcc ggccccttgc ttgaattaag
     1021 gattcggaac tgtattccgg cttcatctgc ggccgcagcc attgcccccc cgagcgcgtc
     1081 cacttccttg acgagatgcc ctttgccgat gccaccaatc gatgggttgc atgacatctg
     1141 ccccagcgtt tcgatattct gggtaagcaa caacgtcctg caacccatgc gagcggccgc
     1201 aagcgccgcc tcggttccgg catgtccgcc gccaactaca atcacatcaa aacgttcagg
     1261 ggaatgcatt tcgattttgg tacaacggga ttaagcgaac cgggaaaaga gggcgattct
     1321 acggcaaaat gaaccatgag ggaaacgcga ccagcgcgtt gcggcgcttt cggtggtcca
     1381 tggttgccca tgtttcacgt gaaacacaaa gccagccagt cgcgagcgga gaagcgtcaa
     1441 tccgaaccgg ttcaattact tcccgatgca gaagcgagaa aatatctcac ccagcaggtc
     1501 gtcggcagta aactcccctg taatcgacga cagtgcaatc tgcgcaagcc gcaattcctc
     1561 cgccagcaac tccagcggct tcccgctccc cgccaggtca tccgcgtgag tcagatgccc
     1621 ggtcgcctcg gacaaggcaa ccaagtgtcg ctgtcgcgcc atgaacaccc cgccccccga
     1681 ttccaggttc caccctgcca gatccagcac cgcatcccgc agcgcttcca ttcccgcacc
     1741 ggttttagcc gatacatata tctctgtttc ccctgaggat gtcggcccgt tccacgccgg
     1801 aagagcgtga tccatcagat cgattttgtt atagacatat accctttgca agttcgctgg
     1861 cagactctct attatcagac ggtcctcttg cgcaaggcct tccctgcagt caagaaccac
     1921 caaagcaagc gccgcctttt caatggccga ccgcgtccgc tcgatgccga tgctttcaat
     1981 caggtcttct gtttcgcgta acccagccgt atcaaccacg tgcatcggaa cgcctttaat
     2041 ttctatcggg cgttcaatgg tatcgcgtgt cgtcccagga acttcggtta cgatagcggc
     2101 ctcctcaccc gtcagctgat tcagcaaact cgatttacca acattcggct tgccaaccaa
     2161 tacaactgaa acaccttcac gcaacagact tccccttctg gcagataaaa aaacctcatc
     2221 cagccgaccc cttatatctc caagccgttc atcaatatgc ggttgggcaa gcacgtcgat
     2281 gtcctcctcc gggaaatcaa gccaggcctc aacgatcatg cgcagatcag tgagtccact
     2341 tacaatttca tgtatgaggg acgaaaactc accattcagc gagcgtaacg cagaacgagc
     2401 tgcctcccgg gttccagcat cgatgaggtc tgcaacgctt tccgcttgcg cgagatccag
     2461 cttgtcgtta aaaaaagcgc gcagcgtgaa ttcccccggc tcagccaggc gcgcgcccgc
     2521 cgaaatacag ctggcaagca ataagctcat gacgatggga cccccatgac cctgaaattc
     2581 cagcacatct tctcccgtat acgaatgagg tgccggaaaa taaagggcaa tgccctggtc
     2641 aatcacttgt ccatcttcgt ccaggaaatg acttagagtt gcgtaccttg gcttgggcat
     2701 ggagccgaca actttctccg ccaataatct gaggtccctg ccagagacgc gcaccacgcc
     2761 tattcccccg cgacctgggg gggtggaaac tgccgcaata atatcggggg ctgggatcaa
     2821 ggcaggtaca gggagggccg ccacaggcgc aatagacgca atagaacggg tttgcgctac
     2881 gatcgagctt atacgcaacc gcagaacatt tgcactatct cgttacgcgg ctttgtctag
     2941 tcgtcgcgct caggcaaggg cgcctgcagc ccccaggctt ttgcattaaa accgcgcgga
     3001 caaccgggca agtcctgtgc gaatcacctt ttcttgctta aagcagtcgt ggccgcggca
     3061 ccctctatca ttcgggtgat ttgccactgc tgggcgatcg acagtatgtt gttgaccagc
     3121 gagtacagga cgagtcccgc agggaagaag aaaaagaaga cgctgaaggc aaatggcatg
     3181 atctgcatca ccttttgctg aatcggatcc gtcgccggcg gactgagctt cgactggatg
     3241 aacatcgaaa ctcccatgat gaccggcaag acataataag ggtccggaac ggataaatcc
     3301 tgtatccata atgcaaacgg cgcattacgg agctcgacgc tggaaaggat gacccaataa
     3361 agcgagatga aaacaggtat ctgcaccact atggggaggc agccgcccat gggattgatt
     3421 ttttcggtct tgtagaactg catcatggct tcgtgcatgc gctgccggtc attcccatgc
     3481 tgctcccgca gccgctgcaa cttcggcgtc acgagccgaa gcttggccat cgagcggtag
     3541 cccgcagccg ataaggggaa aaaggcggct ttgaccgaca tggtcaaaag gataatcgca
     3601 actccccagt tatccgtcca ggagtggaag aacgacaaca gccagaaaag cggcgccgcg
     3661 atgacggtga gccatccata atcaaccgtg aggtccagcc cgggcgcaat cgaggccagc
     3721 ttgctctgtt cctctggccc ggcgtaaagg ggaacgtcga cgaccgcggt ctttcccggt
     3781 tcgatcaaac ccaccggcac gatcacgccc gctgaatact ggttttctgc catgcgcttg
     3841 gcgtaatact cgcgtggctg cttatccttc ggcagccagg ccgtcatgaa ataatgctgc
     3901 agcatggcta tccagccatt gtccgcgttc ttcggatagc ttgcgttacc cttgtccagg
     3961 gcggaaaact cgattttctt gaatttatcc tgctcggtgt agactgccgc ccccgtgtat
     4021 gtcggtatca tgaatatcga accgacgggc ggcttgctgt cgcgcacaat ctggaaatag
     4081 gaaaaaggtt gaatcggcgc tgcccgatga ttggaaacct cgaagctgac atcgatgaga
     4141 tatttcccac gatgaaatgt ataaaccttg gttacctgca ccccatcagc ttccggcgcg
     4201 gacagcctga tctcgacctt atccttgccg ggttcgagac tgtacgtgcc agcctccgcc
     4261 gtatactgcg tcttgtgcgt gggcagacct tccccaatca gccccgactg ggtcacgtag
     4321 agctgatcgc cttggctctc caataatgca aaggacttgt tcttgtcgtc ccggtcagga
     4381 taattgagca attccaggcg gcgcaggtca ccgccggcgg tatcgatctc cgccgtgacc
     4441 acgtcggtct tgacgacaat tttctccccg gattcaagaa tgctggaagg tctccccggg
     4501 gtgatatttt cagcgccagg agtgcttgta ccggcctgcg tggaagaaag tttttcgcca
     4561 ggaatcggag ctacatgctc acctgactga ctggatgacc cctcaactgc tggtttaggc
     4621 aaagccgcat actgctcttt ttgccaggcc tcccacagga acagcatgga ggttgaaaag
     4681 ataaggaaaa gtacgagttt ttgggtatcc atggtacttg cagttttatt ttttgacgaa
     4741 gcgcacgagc ttcccggatg tcaactaggg aacgggatca tccccgcctt gactccacgg
     4801 atggcagcgc atgatccgcc aaatgctcag gcatgaccct cgcatggccc catgcctggc
     4861 cagcgcctca caggcgtaat gcgagcagga aggactgaag cggcatgaag ggcccagaaa
     4921 agggctaagg caatattggt agagcttgat gaaacctatg ataatttgcg acatcgctgc
     4981 aactgaacca tgagttcgtc tgcttccgcc cttattcgcg gagaatcatc ctgcatggtg
     5041 cggcttcgaa gccgcaccac cagatcaagt cctcccaatt catgctgccg cgcacgaaac
     5101 gcctcccgca gtagtcgctt cgtcctattg cgtcttacag acaaacgctc tattttacca
     5161 gcgactatca gtcctaagcg agactgcgaa aggccattgg gtttagcgaa aacctgaagc
     5221 aattcactat tggcggagca tctgaatcga aggaccgaag agaattcttc gggcttgcgc
     5281 aatcggtgag ttcggggaaa acggatattg atccctcacc caaggttttg gcagttcaag
     5341 ctactgccag acgaacgcgc cctttggccc ggcgcgcgcg gataacagcc gccccaccag
     5401 tggttttcat gcgcaccctg aaaccatggg tgcgctttct tcgggtgacg gaaggttgat
     5461 aagtgcgttt catgacgatt cttcctgagg ccgttgaata actcaacatt acaactcttt
     5521 acgcggcctg ctgtcaaccg atattttttc ctcaccctgt ggataagtgc ctgggaacag
     5581 tctagaatac gactcacccc gctcccgaaa ttcctttttt ctcccaagag tattttctcc
     5641 aagagcaatt acgatcatgc ctgcaatgga taacttttgg cggtcttgcg tcggatattt
     5701 cgagaaagaa ctgagcgccc agcaattcaa cacatggatc aagccgctgc gcctccagcc
     5761 ggcaacggga gagacttccg atccgattct cattgcccct aaccgcttcg tcctgcagtg
     5821 gatcaaggat aattttctcg ctcatatcga gcgcatggct gagcagtatt tttgccgcac
     5881 tgttcgcttc cagctggcgc tttccgaccc gctttccaac catgtggccg ccagtccggc
     5941 tcccgtaaac acgatcgcgc ctgccccatc atcccgggcc gatctggatg ctcccgcagt
     6001 agtcataaag aagccgaatg gcgacgcaag ggaaaaaaat gcgagccgcc tgaatgcgtc
     6061 attcaccttt gaaaacttcg ttaccggcag ggccaatcaa ttagcccgcg caggcgccat
     6121 acaggtcgca gagcgtccgg gcgtcgccta caaccctttc tttatttacg gcggtgtagg
     6181 gctgggaaaa acgcacctca tacaggccat tggcaatctg gtgctggagc ataatcgctc
     6241 cgccaaggtg aggtatatcc acgcggaaca gtatgtatcc gacgttgtaa aagcgtatca
     6301 gcacaaggca ttcgacgatt tcaaacgcta ctaccactcg cttgatctgc tgctgatcga
     6361 cgacatacag ttctttggag gcaagaaccg aacccaggaa gaattctttt acgctttcaa
     6421 cgcgctcatc gaagcgcaca agcaggtcat tatcacctgc gactcttatc ccaaggaaat
     6481 ctcgggcatg gaagaacggc tcatttcgcg cttcggctgg ggtctgacag tggcggtgga
     6541 gccgccggaa ctcgaaatgc gggtcgccat cctgctcaag aaagcgctga tggaaaatat
     6601 catgctggat gaaaatgtgg cttttttcat cgccaaacac atacgttcca acgtgcgcga
     6661 gctggaaggc gccctgaaac gggtcgtcgc ctatgcccgc ttcacggggc acgcgttatc
     6721 gctggatctt gcgcgggaag cgctaaaaga cctgctcgca gtgcagaaca ggcagatttc
     6781 aatcgaaaac atccagaaga cagtggccga ttactataaa atcaaggttg ccgagatgta
     6841 ttcaaaaaaa cgttcccgcg ttgttgcacg gccccggcaa atggccatgg caatatccaa
     6901 ggaactgacc cccctcagcc tgcccgatat cggcgaagcg ttcggaggca gggaccatac
     6961 aaccgttctc cacgggtatc ggaaaatcgc cgaactgcgc gcgtccgacc cggcaatcaa
     7021 tcgcgacttc aatacactgt tacacattct ccgcggctga tttccccttg ataagaaaag
     7081 gacaaaatgg ggataactcg gactcttagt cttccggccg tttctgctca caaataatcc
     7141 acaggcaata cctcgacaat acaggcccaa aatagcaccc aagctgttga gattgcgcca
     7201 gtttgcagat tcataccgga gttgttccaa gactattaac gattattggg gttatataac
     7261 atgaaattaa tacaaaccga gagagacata ttgctcaagc ccctccaggt tgtgacgggc
     7321 atcgtggaac gccggcatac gctgccgata ctctccaacg tgctcattga gcgaaaagac
     7381 gacaggattt cctttgttgc aacagacctc gagatccaga taacgaccag cgcgcaggat
     7441 ggggcgactg ctagcgaaga ccacggcgtg actgtcgccg cgaaaaagct tcaggacata
     7501 ttgcgcgcgc ttcctgataa aacgcaggta gctcttgaaa ccgaagggtc tcggctgcat
     7561 gtgaaagcgg gaaaaagccg cttccatctc cagacgcttc ccgtcgagga tttcccaaaa
     7621 tttccggcag gaagcgagcc ccaggagaaa ataaccttaa aacagaagga gttgaagcat
     7681 ctgttgtcaa tggttcagta tgcgatggcc cagcaggata tccgctatta tttgaatggt
     7741 ttgctcctgc ttacggaaga ccatcaattg aaggttgttg cgaccgacgg ccaccgcctc
     7801 gcgttcgcgg tgatgccgct tgaggccggc gaggagaatc cggaagcaag aagggaagtg
     7861 attctgccgc gcaaggtcgt cctcgagttg acgcggctct tgaacgacac ggacgagccg
     7921 gtcaccgtgg agatgctgca aaaccaggta cgtttcacct tcggagccgt tgtactggtc
     7981 accaaggtgg tggatggaaa atttcctgat tacaaccgcg tcattcc
//
LOCUS       DASSHJ010000188         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1576053,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000188 DASSHJ010000000
VERSION     DASSHJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576053"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFI43_07295"
     CDS             complement(<1..479)
                     /locus_tag="VFI43_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7061967.1"
                     /translation="MTHPSDPAAGNPIARIHGEAITEIPRDLYIPPDALEVFLDTFQG
                     PLDLLLYLIRKHNLDVLDIPMAELTRQYLDYIEMMRANRLELAAEYLLMAAMLIEIKS
                     RMLLPKPAEPPQDEAGDPRAELVRRLLEYEQMKKAGRRLDELPQAERDFSLVQVWID"
     gene            complement(573..1775)
                     /locus_tag="VFI43_07300"
     CDS             complement(573..1775)
                     /locus_tag="VFI43_07300"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7061968.1"
                     /translation="MFADRIVSGMRPTGGLHLGHYHGVLKNWIELQHQFECLFFVADW
                     HALTTHYDTPEVIEQHVWDMVIDWLAAGVDPAQATLFIQSRIPAHAELDVLLSMITPL
                     GWLERVPSYKDQQEKLAEKDLSTFGFLGYPLLQSADILVYRATRVPVGEDQVPHIELT
                     REIARRFNHVFGKDPGFREKAELAIRKLGSKKSRVYRELRNRYQEQGDEEALAAAKSL
                     LDAQQNLSLGDQERLFGYLEGGGKMILSEPEAMLTPESKMPGLDGQKMSKSYNNTINL
                     LEEPASVIKKIRTMPTDPARVRRNDPGDPNKCPLWQFHLVYSSPEIKEWAYNGCITAG
                     IGCLDCKQPVIDAILAEQGPMRERAQIYEEDPTLVRSIIADGCERADKLADETMREVR
                     EAMGLDYS"
     gene            complement(1824..2474)
                     /locus_tag="VFI43_07305"
     CDS             complement(1824..2474)
                     /locus_tag="VFI43_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7061969.1"
                     /translation="MDNIIQTIAIYALPVIFAITLHEAAHGYVAKHFGDLTAYAQGRV
                     SLNPIRHIDPLGTILLPLATLLLGGILFGWAKPVPVNFSALRRPKQDMLWVALAGPGA
                     NLFMALLWGLVIRVGLAMSESDLARPMILMGEAGIKINIVLMVLNLLPLPPLDGGRIA
                     VSLLPMRMAQSFARIEPYGFMILLLLLLTGVLGAIIGPFIAIGIRMVAWFFGLGTE"
     gene            complement(2467..3099)
                     /locus_tag="VFI43_07310"
     CDS             complement(2467..3099)
                     /locus_tag="VFI43_07310"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET7061970.1"
                     /translation="MAQFFSVHPDNPQPRLIRQAAAIIRDGGLIVYPTDSCYALGGHL
                     GDKSIMGRMRAIRQVDEHHHFTLMCKDLTEVSHYARFDNFQYRLLKACTPGCYTFIFQ
                     ATREVPRRLQHPKRNTIGVRIPDHPVVQTLLSELGEPLLSSTLILPGEELPLNDPSEI
                     RERLEHHVDLVLDAGACGVETSTIIDLTGNAPELVRQGRGSLAPFGFSHG"
BASE COUNT          737 a          957 c          890 g          753 t
ORIGIN      
        1 tcatcaatcc acacctgtac cagcgaaaaa tcccgttctg cttgcggcag ctcatcgagc
       61 cggcgccctg ctttcttcat ttgttcatat tccagcaacc ggcgcaccaa ctccgcgcgg
      121 gggtcgccgg cctcgtcttg gggcggttcc gcaggcttgg gcagaagcat gcgcgattta
      181 atttcaatca acatcgccgc catcaagagg tattccgcgg caagttccaa gcggtttgca
      241 cgcatcatct cgatatagtc gaggtattgc cgggtgagtt cggccatggg aatatccagc
      301 acatccaggt tgtgcttgcg aataagataa agcagaagat cgagcggccc ttgaaatgta
      361 tcgagaaaca cttcaagcgc gtccggggga atgtatagat cccggggtat ttccgtaatt
      421 gcctcgccat ggattctcgc gatcgggttg ccggcagcag gatcggatgg atgagtcatc
      481 ctgacactat ccagttcagc ttgtggtata ggcacggtag ttcttagggg tggggctcgc
      541 ttgaagctgc aagttccaga tctgctcaaa tctcacgaat agtccagccc catcgcctcc
      601 ctgacctcgc gcatcgtctc gtcagccagc ttgtctgctc tctcgcaccc gtcggcaata
      661 atgctgcgca ccaacgtcgg gtcctcctcg tatatctggg cacgctcgcg catcgggcct
      721 tgctctgcga gaatcgcgtc gataaccggc tgcttgcagt ccaggcaacc tatccctgcc
      781 gtgatgcaac cgttataggc ccattccttg atttccgggc tggaataaac cagatgaaac
      841 tgccataaag gacatttgtt gggatcgcct ggatcgttgc gccgaactct cgccgggtcg
      901 gtcggcatcg ttctgatttt ttttatgacg ctggccggtt cttccagcaa attgatcgtg
      961 ttgttatatg acttcgacat cttctggcca tccagccccg gcattttcga ttccggcgtc
     1021 agcatcgctt ccggttcgga caggatcatt ttaccgccgc cttccagata tccgaacagt
     1081 ctttcctggt ctcccaggct caaattttgc tgcgcatcga gcagcgattt tgccgcggcc
     1141 aaagcttcct catccccctg ttcctggtag cgattgcgca gttcccggta aactctcgat
     1201 tttttcgacc ccagcttcct gatcgccaat tccgcttttt cacgaaaccc cgggtccttg
     1261 ccaaagacat gattgaagcg gcgtgcaatc tcccttgtga gctcgatatg cggtacctgg
     1321 tcctcgccta ccggcacgcg ggtagcccgg tagacgagaa tatctgcgct ctgcagcagg
     1381 gggtagccga gaaaaccgaa tgtgctcaag tccttttccg cgagcttctc ctgctggtct
     1441 ttatatgagg gcactctttc aagccatcca agcggcgtaa tcatggaaag gaggacatca
     1501 agttccgcat gcgctggtat gcgcgactgg atgaataaag ttgcctgcgc ggggtccacc
     1561 ccggctgcaa gccagtcgat caccatatcc cacacgtgct gctcgatcac ctcaggggta
     1621 tcgtaatggg tagtgagggc atgccaatcc gcaacgaaga acaggcattc gaactgatgt
     1681 tgcaattcaa tccagttctt cagcacccca tgataatggc ccaagtgcag gccgccagta
     1741 ggccgcatgc ctgaaacaat tcgatcagcg aacattcatt aatccttact cgatcctcat
     1801 gagtttattc attcagcagt gacttattca gttcccaaac cgaaaaacca ggccaccata
     1861 cgaatcccaa tggcgataaa gggaccgata atcgcgccga gtactcccgt cagcaacagc
     1921 aacagcaaaa tcatgaatcc atacggctcg atcctggcaa agctttgcgc catccgcatc
     1981 gggagaaggc ttactgcaat gcgccctccg tcaagtggcg gtaagggcag cagattcagc
     2041 accatcagca cgatattgat ctttatcccc gcctcgccca ttaatatcat tggccgagcc
     2101 aggtcgcttt cggacattgc gagtcccacc ctgatcacca gaccccacag caaggccatg
     2161 aacaaattgg cgcccgggcc cgcaagggca acccataaca tgtcctgctt gggccttcgc
     2221 aaggccgaaa aattgacggg taccggtttg gcccagccaa acaggatccc gcccagtaac
     2281 agcgtggcta acggaagaag aatggtacct agaggatcga tatgccggat cgggttcagg
     2341 ctgacgcgcc cctgagcata ggcggtgaga tcgccgaaat gcttggcgac atagccgtgg
     2401 gccgcttcat gcagggtaat cgcaaagatg accggtagcg cataaattgc gatagtctgg
     2461 ataatgttat ccatgggaaa acccgaaagg agcaagactc cccctacctt gccgcaccag
     2521 ctcgggagca ttcccggtca aatcaattat cgtgctcgtt tccactccac atgctccggc
     2581 atcgagtaca agatccacgt ggtgctccag gcgttcgcga atctcagagg gatcgttaag
     2641 gggcagctcc tcaccgggca ggatcagtgt agaacttagc agcggctcgc ccaactcgga
     2701 cagcagtgtc tgcacgaccg ggtgatcagg aatccggacg ccaatggtat tgcgcttcgg
     2761 gtgctgcaac cggcggggca cctcgcgcgt cgcctggaat ataaaggtgt agcaaccggg
     2821 cgtgcatgcc ttcagcagtc tgtactggaa attgtcgaac cgggcgtagt gcgagacttc
     2881 cgtcagatcc ttgcacatca gggtaaagtg gtgatgttcg tccacttggc gaattgcgcg
     2941 catacggccc attatacttt tgtcgcccaa atggccgcca agcgcgtagc atgaatctgt
     3001 aggatatacg atcagccctc cgtccctgat gatggccgcg gcttgtctta tcaagcgggg
     3061 ctgtggatta tcaggatgta ctgaaaaaaa ttgagccact caggggaggt ttctggtttg
     3121 gttctttttc ggcgcgaaat tcaggcttcc gcctcgcacc gtggcaatag agagccggag
     3181 gttcgtgcat ccgtacatcc gaggcagagg cggacaaagg caggactgaa tggcgaggcg
     3241 tagcgatgca atggcgatcc ctcgggacgg ctgcgtccca tcatgccgtt cccttccagt
     3301 catgccatac cgggacgcaa tgggcgggga gttcagg
//
LOCUS       DASSHJ010000189         8780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1232596,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000189 DASSHJ010000000
VERSION     DASSHJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232596"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFI43_07315"
     CDS             <1..241
                     /locus_tag="VFI43_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7061971.1"
                     /translation="QPGFVALLCRHLKTGGYIHVATDWEEYAEQILEVLDREPQLNNT
                     AASYASRPEYRPLTRFEQRGLKLGHNVRDLIFRKN"
     gene            complement(262..1491)
                     /gene="hemW"
                     /locus_tag="VFI43_07320"
     CDS             complement(262..1491)
                     /gene="hemW"
                     /locus_tag="VFI43_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET7061972.1"
                     /translation="MSSTVSLASLSASQAPRLKSLPPLSLYVHIPWCVRKCPYCDFNS
                     HEVRGRVPDLPEEEYVAALVSDLEQSIPTVWGRNVVSVFFGGGTPSLFSAQSIDAILS
                     AARALLTVELLAEVTLEANPGTFEAQKFADFRSAGINRLSVGIQSFNSRHLQALGRIH
                     DGDDARRAIEIAQKNFDNVNLDLMYGLPDQSLDEARSDIETGVSSGVSHISAYHLTVE
                     PNTLFHRYPPTLPDDDLAAEMQIMIEQTLRENGYVNYETSAFAQSGRESRHNMNYWLF
                     GDYLGVGAGAHSKISFRERIIRQVRYKQPKEYLSRAASGMSDSAALIMEQHEVTRSDR
                     IFEFMMNALRLTTGFAAETFVERTGVPITAIQRQLDEAERGGLIERDYRRIAPTLTGK
                     RFLNDLLQIFLPQKSRT"
     gene            complement(1594..2187)
                     /gene="rdgB"
                     /locus_tag="VFI43_07325"
     CDS             complement(1594..2187)
                     /gene="rdgB"
                     /locus_tag="VFI43_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET7061973.1"
                     /translation="MRKLVIASSNAGKLREISHLLAPLGIEAMPQSAFNVSETDEPYS
                     TFVENALTKARHSSEFTGLPSLADDSGICVNALNGSPGVLSARYAGEPRSDERNNLKL
                     VSALQNQADCRAHYYCVIVLVRHPVDPQPIIADGCWHGEIVMEPRGQGGFGYDPYFFL
                     PALGQTAAELPMEQKNRISHRGKALARLIERIREETL"
     gene            complement(2213..2932)
                     /gene="rph"
                     /locus_tag="VFI43_07330"
     CDS             complement(2213..2932)
                     /gene="rph"
                     /locus_tag="VFI43_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7061974.1"
                     /translation="MRPSRRSSAQLRPVEIIRHYTKHAEGSVLIACGDTKIVCTASID
                     ESVPPFLKGKGQGWLTAEYGMLPRSTGERMQREAAKGRQSGRTMEIQRLIGRALRSVV
                     DLTKLGERTIQIDCDVIQADGGTRTASITGAFVALHDAVDTLMRRQMILATPILSHVA
                     AVSVGVYEGTPVLDLDYEEDSRCDTDMNVVMTGNSGFVEMQGTAEGMPFTRDDLNAML
                     DLAQQGIRELIELQKKALASA"
     gene            3116..3982
                     /locus_tag="VFI43_07335"
     CDS             3116..3982
                     /locus_tag="VFI43_07335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET7061975.1"
                     /translation="MIYSMTGYAMVTRELPQGSLNLELRSVNNRYLDIQFRLPDELRS
                     LEAAMREFLVSRLNRGKVECRINFSRLPESDDLQLINSNLLQKLLELDRTVRAALPEA
                     RGLEVADILRWPGILGGTPQTLENLREPCIDLLQAALDDFAASRSREGEKLKAVLLER
                     IYRMRELVSNVAPRIPALLAAFQEKLKARLQEAMINCDDERIRLELGLFASKIDVDEE
                     LSRLTAHLEEVERILCRGGAIGKRLDFLMQELNREANTLAAKSVDAEVSKISVELKVL
                     IEQMREQVQNIE"
     gene            4420..4650
                     /locus_tag="VFI43_07340"
     CDS             4420..4650
                     /locus_tag="VFI43_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061976.1"
                     /translation="MGSQVYKEIPMREYKITFIPINPAEAPVGPVIVTGSSLDEACHK
                     AVERLKNENPSRPYDAKDYKPFPQISWQELPE"
     gene            4854..5291
                     /gene="trxC"
                     /locus_tag="VFI43_07345"
     CDS             4854..5291
                     /gene="trxC"
                     /locus_tag="VFI43_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HET7061977.1"
                     /translation="MSDFSHVVCPNCQAINRVPEQRLDQHPNCGQCHQPLFTGHPVEL
                     TQASFAKHLDRDDIPLLVDFWAPWCGPCRAMAPAFAQAAAILEPHARLAKVNTDNEQA
                     LGARYNIRSIPTLILFRSGREVARQAGAMGAQDIVRWVQSHQR"
     gene            5376..5990
                     /gene="gmk"
                     /locus_tag="VFI43_07350"
     CDS             5376..5990
                     /gene="gmk"
                     /locus_tag="VFI43_07350"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7061978.1"
                     /translation="MTATPGSLFIVSAPSGAGKTSLVKAMLRSGVDLSLSVSHTTRPA
                     RSQEVEGRDYYFVSRENFEEMQRNGEFLESAQVYGNYYGTSKKWIDEAIASGRDVLLE
                     IDSQGARQVRQVFPQAVSLFVLPPSLQVLELRLRSRAQDSLESIARRLAAARDEVSHV
                     GEYDYVIINETLEQAAQDFKSVVQAERLRLAKQLVRHRDLLEQF"
     gene            6135..6341
                     /gene="rpoZ"
                     /locus_tag="VFI43_07355"
     CDS             6135..6341
                     /gene="rpoZ"
                     /locus_tag="VFI43_07355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7061979.1"
                     /translation="MARITVDDCLKRIPNRFDMTLAATIRARQLSIGGTPMVEPARDK
                     PTVIALREVAQGKIGPDILTTGAL"
     gene            6489..8636
                     /locus_tag="VFI43_07360"
     CDS             6489..8636
                     /locus_tag="VFI43_07360"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7061980.1"
                     /translation="MQVRSLPNSSATSPEAGFLFSELSSYLKREDVYELQNVYLFSKS
                     AHSGQFRQSGEPYISHPLAVASILGKLRLDTQTLAAALLHDVMEDTHVSKSEISDRFG
                     KPVAELVDGVSKLDKIEFQSYADAQAENFRKMLLAMAQDVRVILIKLADRLHNMRTLE
                     AMRPEKRRRIARETMEIYAPIANRLGLNNIYHELQDLSFRYAFPTRYRVLANATKAAR
                     GNRREMVTKILEAMRQRLKTAGLDAEVSGREKQLYSIYKKMAEKHLTFSEVLDIYGFR
                     VIVKDVPSCYIALGALHSLYHPIPGKFKDYIAIPKANGYQSLHSTLWGPFGTPLEVQI
                     RTPEMHRIAEAGVASHWLYKSSDADLNDLHIKTNQWLQSLLETLSDSSDSLEFLEHIK
                     VDLFPGEVYVFTPQGKILALPKNSTAVDFAYAVHTDIGDCCVAAKINGESAPLRTALK
                     SGDQVEIVTASHAKPNPAWLNYVVTGKARSHIRRFLKTMQYEESARLGERLLNQALAS
                     LRIDPQNITLAQWEKLARESGAKSKQDLLADIGLGKHLPAVIARRLGSPVESVSGATT
                     NIDVITILGTEGMAVQFAKCCRPIPGDPVVGFIKKDQGLVIHIDDCPAIPKNRGHSDN
                     WLDVAWGKNITRQFETSIRLMAANKRGVLARVAAGIADSGSNIDNVAMEGDGAYTTMT
                     FALQVRNRLHLAQVMRTLRRIPEVERIIRVKGS"
BASE COUNT         2094 a         2303 c         2430 g         1953 t
ORIGIN      
        1 ccagcccgga tttgttgcgc tgctctgccg ccatctaaag actggagggt atattcatgt
       61 cgcgaccgat tgggaggagt acgcagagca gatactggaa gtgttggacc gggagccgca
      121 acttaacaac acggctgcga gttatgcgtc gcggccggaa taccggccac tgacaaggtt
      181 tgaacagcgc ggcctgaagc tgggccacaa cgtgcgagac ctgatatttc ggaaaaatta
      241 gacgaaagcg ttcatgcagc tctacgtccg gctcttctgt ggaagaaata tctgcaacag
      301 atcgttcagg aagcgcttcc ccgtaagggt gggtgcaata cgacggtaat cgcgctctat
      361 taagcctccc cgctccgctt catcaagctg gcgctggatt gcagtaattg gcactccggt
      421 tcgctcgaca aaagtttcag ccgcaaagcc ggtcgtaaga cgaagcgcat tcatcatgaa
      481 ttcaaatatc cggtcgctcc gcgtaacttc atgctgttcc atgatcaggg ctgcggaatc
      541 ggacatgccg gatgcagctc tggaaagata ttctttgggc tgtttgtagc gaacctgacg
      601 gataattctc tcgcgaaagc tgatcttgct gtgcgcgcca gccccgacgc ccaggtaatc
      661 accgaacagc caatagttca tgttatggcg tgattctcgt ccagactggg caaatgcgga
      721 agtttcgtag ttgacgtaac cattttccct gagggtttgc tcgatcatta tctgcatttc
      781 cgccgcgagg tcgtcgtcag gcagtgtggg gggataccgg tgaaagagcg tattgggctc
      841 aaccgtcagg tgatacgcgg aaatatggga gacccccgag gaaaccccgg tttcgatgtc
      901 gctgcgcgct tcatcaagtg actgatcggg caagccatac atgagatcga ggttcacatt
      961 atcgaaattc ttctgggcaa tttctattgc ccggcgggca tcgtcgccat catgtatccg
     1021 gcctagcgcc tgaagatgcc gggagttgaa gctctggatt cccaccgata accgattgat
     1081 gcccgcgctg cgaaaatccg cgaacttttg agcttcgaaa gtgccggggt ttgcctccag
     1141 cgttacctcg gcaagcaact ccaccgtcaa cagggcgcga gcagccgaaa ggatggcgtc
     1201 aattgattgg gcgctaaaca ggctcggcgt gccgcctcca aagaatacgc ttactacgtt
     1261 gcgaccccag acagtgggaa tcgactgttc gagatcgctt acgagggccg caacatactc
     1321 ctcctccggc aaatcgggaa cccgtccacg aacttcatgc gaattgaaat cacaataagg
     1381 acactttctc acgcaccagg gtatatggac atagagactc aggggcggca gagactttag
     1441 ccgcggtgct tgtgaagcgg ataaggaggc gagagaaaca gtggatgaca tggcagtccg
     1501 gcagtttttc taaagtggaa tacagtggaa aaggcgctgt ttcagttaat cactgcgacg
     1561 atccacattc tgcgtcccgt ccacaaaaaa agctcagaga gtttcttccc tgattcgttc
     1621 gatcagccgc gccagcgctt ttccccgatg gctgattcga tttttttgct ccatcggaag
     1681 ttccgctgca gtttgcccta acgccgggag aaaaaaatag ggatcgtagc caaacccgcc
     1741 ctgtccccgt ggttccatca cgatttcccc gtgccagcac ccatcggcaa taatcggctg
     1801 gggatcgacc ggatggcgaa cgagtacaat cacgcaataa tagtgggccc tgcaatccgc
     1861 ctggttttgg agtgcggaaa ccagtttcag gttgtttcgc tcatcagatc tgggttcgcc
     1921 ggcatagcgc gccgacagta cgccgggact gccgttcagg gcgttgacac agatccccga
     1981 gtcatcggcc agggaaggga gtccggtgaa ctcggatgag tgccgggctt tcgtgagcgc
     2041 attctccacg aaagtggaat agggctcgtc agtttcgctg acattaaacg cggactgggg
     2101 catcgcttcg attcccaagg gcgccagcag atggctaatt tcccgcaatt tgcctgcgtt
     2161 gctgctcgct ataaccagct tcctcatatc tttcatgaac gccggttcga catcacgcgg
     2221 atgccaatgc ctttttttga agctcgatca gctctcgtat gccctgctgt gccagatcga
     2281 gcatcgcatt caggtcatcg cgagtaaatg gcataccctc agcagttccc tgcatttcga
     2341 cgaaacccga attgcctgtc atgacgacat tcatatcagt atcacagcgg gaatcctctt
     2401 cgtagtcgag atcgagaact ggagtgcctt catagacgcc gaccgagacg gcggcaacgt
     2461 gcgaaaggat gggggtcgcc agtatcatct ggcggcgcat cagcgtgtcc accgcatcat
     2521 gcagcgcgac aaaagcgccc gtaatactcg cagtccgggt gcctccatcc gcctggatga
     2581 cgtcacaatc gatctgtatg gttcgttcac ccagtttcgt cagatccact actgaacgca
     2641 gggctcgccc aatcaagcgc tgaatctcca tggtacgccc tgattgcctg cctttcgccg
     2701 cttcacgctg catgcgctcc ccggtcgaac gcggaagcat cccatactca gcagtaagcc
     2761 aaccctggcc ttttcccttg agaaacgggg ggacgctctc gtctatgctc gccgtgcata
     2821 cgattttcgt atcgccgcac gcgatcagga ccgaaccttc ggcatgtttg gtgtaatggc
     2881 ggattatctc gacgggacga agctgagcgg aagaacggcg gcttgggcgc atggcggaaa
     2941 tcggtttttt ataacggaag atcggagttc ctggtctcga ttacagacga tcgcagcaag
     3001 ctgcctggca attcgaggga gagtgcgggc tgcgggccca ctatggcata atctgctctg
     3061 cggatgaact cattatttta acgcataggg aaatggcgag caggcgaagt taaccatgat
     3121 ctacagtatg actggttacg ccatggttac cagggaattg ccacagggct cactcaacct
     3181 ggagctgcgc tctgtcaaca accggtatct cgacattcag ttccggctgc cggacgagct
     3241 tcgttcgctt gaagcggcga tgagggaatt tctcgtctcc cggctgaatc ggggaaaagt
     3301 cgagtgcagg atcaattttt ctcggctacc ggaaagtgac gatctgcagc ttatcaattc
     3361 gaacctgctc cagaagctac tggaactgga tcgaaccgtc agggctgcgc tccccgaagc
     3421 aaggggattg gaagtcgcag acatccttcg ctggccaggt atcctgggag gaactcccca
     3481 aacgctggag aatctacgcg agccttgcat tgacctgctt caggcggcat tggacgattt
     3541 tgcagcttcc agatcccggg agggagagaa gctcaaggct gtgctgctgg agcgtatata
     3601 caggatgcgt gagctcgtta gtaacgtcgc tccgcgtatc cctgccttgc tcgctgcctt
     3661 ccaggaaaaa ttaaaggcac gcctgcaaga agcgatgata aattgcgatg atgagcgtat
     3721 ccgcctggaa cttggcctat ttgcaagcaa gatcgacgtg gatgaagaac tgtcgcgcct
     3781 gaccgctcat cttgaagagg tagagcgcat attgtgcaga gggggggcaa ttggaaagcg
     3841 cctcgatttc ctgatgcagg agctcaatcg cgaagccaat acgcttgcgg caaaatcggt
     3901 cgatgccgag gtatcgaaaa tatcggtcga gcttaaagta ttaatcgaac agatgcgtga
     3961 acaggtgcaa aacatcgaat gaggtttcgg ccacccctat ctcggaagga gaaaggcgga
     4021 ttccttcacc gcctgtgtgg catttattga aggtgggttt gtgaaggagc gcacaatgca
     4081 ggaacccatg ctgtggaata agctggacta agcggcaatc tggttgagtc agacgaagtg
     4141 aaaggcatgg acagttatca agtattgaaa gctgcgataa gctatcctaa acctcttcgc
     4201 cggagcagac ctcagaaaac ctacttaaag gtcatcatgc ctaaagatac tgtttttggc
     4261 gtgaccggtg caataaccct gacagcctga agcggttcac ttttcgtagc gtgccaggca
     4321 atcaaaaccc tcagctcaaa agtctggggg ttttgttttt cctggttgaa agagtgcaga
     4381 cgcctgcatt ttcttgatgt ttttattttt aggaatatca tgggtagtca ggtttataag
     4441 gaaataccga tgcgcgaata taaaattacc tttatcccaa ttaaccctgc tgaggctcca
     4501 gtcggcccgg taattgttac ggggtcatca ctggatgaag catgtcataa ggcggtcgag
     4561 agattgaaga atgagaaccc gagccgccca tatgatgcaa aggattacaa gccgttccct
     4621 caaattagct ggcaagaact gccggaataa atcgcggggt tggagtccga ttatctattc
     4681 cagcgatgat ttctgccaaa cggataccgc cggttgacat gtacgccggt tgacatgtac
     4741 ccctgcgtat acctttagca aaggttgagt aatacaaagc caatagggat aagcgtttga
     4801 tgactctagt tcttgttcag gtgcatcgaa tagttcccga gagatgattc cccatgagcg
     4861 acttctcgca tgttgtgtgt cccaactgcc aagctatcaa tcgtgttccc gagcagcggc
     4921 ttgaccagca tcccaattgt ggacagtgcc atcagcctct cttcaccggg caccccgtag
     4981 aacttaccca ggcgagcttc gccaagcacc tggaccgtga cgacattcct ctattggttg
     5041 acttttgggc gccctggtgc ggcccatgca gggcaatggc ccctgcattc gcgcaagcag
     5101 cggcaatcct tgagccacat gctcgcctgg ccaaagtaaa caccgataac gaacaggctc
     5161 taggggcgcg ctacaatatc cggagtattc ccacgcttat cctgttcagg agcgggcgcg
     5221 aggtggcgcg ccaggccgga gcaatgggag ctcaggatat cgtccggtgg gtgcaatcac
     5281 atcagcgctg aactttttaa cttatggagc ggggagtcga acgggactgc gagggagttg
     5341 cgggagctga tcaccacatt gctttgggat atatcatgac agctacgccg ggcagcctgt
     5401 ttatcgtcag tgctccatcg ggcgcgggaa agaccagtct cgtcaaggca atgcttcgat
     5461 ccggcgttga tctgagcctt tcagtatccc ataccactcg gcctgcccga tcccaagaag
     5521 tcgaagggag ggattactat tttgtgagtc gggagaattt cgaggaaatg cagagaaacg
     5581 gcgagtttct tgaaagcgct caagtctatg gaaactatta cggcacctcc aaaaaatgga
     5641 tcgatgaggc gattgcatcc ggacgtgacg tattgctgga aatcgatagt cagggtgctc
     5701 ggcaggtgcg ccaggttttt cctcaagctg tcagtctctt tgtcctgcct ccctcgctgc
     5761 aagtgctgga attacgcctg agaagccggg ctcaggacag tctcgaatcc atcgcccggc
     5821 gcctcgccgc agctcgcgac gaggtcagcc atgttggcga gtatgactat gttattatca
     5881 acgagacctt ggagcaggca gcacaggact ttaaatcggt tgttcaagcc gagcggttga
     5941 gactcgcaaa gcaactggtc cggcaccgcg atctcctgga acaattttga agcgttactg
     6001 atcatagtca ttatacggct ttggatcagc ggtagcgtta cgcaaggctt tggtttatag
     6061 cttctcttat ggttgccctt gatccaaggc gcgttctata ctgttatttc ttatgcattg
     6121 acgggggtat tttcatggcg cgcattacag ttgatgattg tttaaaaaga attcccaacc
     6181 gtttcgatat gacgttggcc gcgactatca gggcaaggca gctttcgatc ggcggtactc
     6241 ccatggtgga accggcaaga gacaagccta ccgtaatcgc attgcgggaa gtcgcccaag
     6301 gtaaaatcgg acctgacatt cttacaaccg gcgctctttg agacatcccg ccatctgtcg
     6361 aagcctgctc tgaaagcgtt accgcgccag ccagcctcac cggacaacca ggcaatatca
     6421 tgccgcactg acttatcccg ataacttctc aagcaagggt gctttgagct gagatgaagg
     6481 aaacgaccat gcaagtgaga agtttgccga attcctctgc tacttcgccc gaggccgggt
     6541 tcctgttcag cgaactgtcg agctacctga agcgagagga tgtttacgag cttcagaacg
     6601 tatacctttt cagcaagagc gctcattcag gccagttccg ccaatcgggg gaaccttaca
     6661 tttctcatcc gctggcggta gccagcatac tgggcaaatt gcgtctggat actcagacgc
     6721 ttgcggcagc cctgcttcat gacgtgatgg aggatacgca cgtctcgaaa tccgagatca
     6781 gcgacaggtt cggaaaaccg gtcgcggagc tcgtcgatgg ggtttcaaaa ctcgacaaga
     6841 ttgaatttca gagttacgcg gatgcgcaag cggagaattt tcgcaagatg cttctggcaa
     6901 tggcgcagga tgtccgggtg atactcatca agctggccga ccggctgcac aacatgcgca
     6961 cgctggaggc aatgcgcccc gagaagcggc ggcgcattgc gcgcgaaacc atggaaattt
     7021 acgcgcccat tgccaaccgg ctagggctca acaatatcta ccacgaattg caggatctaa
     7081 gctttcgcta cgcttttccc acgcgctatc gcgttctcgc caacgccacc aaggcggcac
     7141 gtggtaaccg gcgcgaaatg gttaccaaga ttcttgaagc aatgagacag cgcctgaaaa
     7201 ctgcagggct ggatgcagag gtcagtgggc gtgaaaagca gctttatagc atttacaaga
     7261 aaatggcgga aaaacacctc accttttcag aggtgctcga tatctacggc tttcgcgtca
     7321 tcgtcaagga cgtgccttcc tgttacattg ccctgggcgc tttgcatagc ctctaccatc
     7381 ccattccggg aaaattcaag gactatatcg ccattccaaa agcgaatggc taccagtcgt
     7441 tgcatagtac cttatgggga ccgttcggca ccccactcga agtccagata cgcaccccgg
     7501 aaatgcaccg tattgccgag gccggcgtgg cgtcgcactg gctttacaag agttctgatg
     7561 cggacctcaa cgatctgcac ataaaaacca accagtggct acaaagtctg ctggaaaccc
     7621 tcagcgactc ctccgattcc ctggaattcc tggaacacat caaggtagac cttttcccgg
     7681 gcgaagtcta cgtgttcacg ccgcaaggaa aaatcctcgc gcttcccaag aattctactg
     7741 ccgtcgattt cgcctacgcg gtgcataccg atatcgggga ttgttgtgtg gctgcaaaaa
     7801 tcaacgggga gagtgcgcct ctgagaacgg ccctgaaaag cggcgaccag gtcgaaatcg
     7861 tgacggcgtc gcatgcaaaa cccaaccctg cctggctgaa ctacgtagtg acgggcaagg
     7921 ctcgctctca catccggcgt tttctcaaga caatgcaata cgaagagtca gcgcggctcg
     7981 gggagcgcct gctcaatcag gcgctcgcct ctctcaggat tgatccccag aacatcacct
     8041 tagctcagtg ggaaaagctt gcccgggaga gcggcgccaa atcgaagcag gaccttcttg
     8101 cggacatagg gcttggaaag cacctgcctg ctgtgattgc aagaaggctg ggttcacccg
     8161 tagagtcagt ttccggcgca acaacaaaca tcgatgtcat tacgatactc ggcacggaag
     8221 gcatggcagt gcagtttgcg aaatgttgcc ggccgatacc gggagacccg gtcgtcggct
     8281 ttataaagaa agatcaaggg ctcgtaattc atatcgatga ttgccccgct attcccaaaa
     8341 acaggggcca ctcggataac tggctggacg tggcctgggg taaaaacatt acgaggcagt
     8401 tcgaaacgag catccggctg atggcggcca acaagcgggg agtactggcg agagtggcgg
     8461 ctgggattgc agactccggt tcgaatattg acaacgtcgc aatggagggc gatggggcgt
     8521 acacgacgat gactttcgca ctgcaagtcc gcaaccgtct tcatctggcc caagtaatgc
     8581 gcacattgag gcgtattccg gaagtggaga gaatcatccg ggtaaaggga agttaataaa
     8641 gggaagttaa aggagagagg tgaggggaga aagcggaaac taccaccttc cctccctcgc
     8701 ttgtggggga ggggcggggg gagagggcag gctcgcgcgg agcatgagag atcagcgaaa
     8761 gcatctccat gtggttcgcc
//
LOCUS       DASSHJ010000190         7291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_174006, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000190 DASSHJ010000000
VERSION     DASSHJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174006"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..1569
                     /gene="ald"
                     /locus_tag="VFI43_07365"
     CDS             451..1569
                     /gene="ald"
                     /locus_tag="VFI43_07365"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7061981.1"
                     /translation="MLIGLPKETKDHETRVGMTPSTVRALTRSGHEVRVQRGAGEGSF
                     LADEEYVAAGAVIVPQAEDAWAAHMVVKVKEPTPAEYRYLHQGMILFTYLHLASDKAL
                     TCALVDTRVTAIAYETVQTEAGKLPLLTPMSEVAGRMAVLVGATYLQRTHGGRGVLLG
                     GVPGVESANVAILGGGIVGTNAARIAVGCGAQVTLLDVNHDRLKYLDDIYRGQLQTRF
                     SDEYNLEDAVFNADLVIGAVLIPGGRAPWLITAGMLPKMRKGSVIVDVAVDQGGCVET
                     TRPTTHSNPTYEIDGVVHYCVANMPSAVPRTSTFALNIQTAMYTVLLANEGLDAVRKN
                     RALQFGLNTYGGSVTYPAVATEFGMKYTEPLEALAGGD"
     gene            1647..2582
                     /locus_tag="VFI43_07370"
     CDS             1647..2582
                     /locus_tag="VFI43_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7061982.1"
                     /translation="MKIRYFPFFAVLVAPFFTAGQAALANVGIAFDYRYDSSGFFTGA
                     NSGRQNLLESAASVFENRFQDNLTEITSSGSNQFTAKLFEPDNGKNISIPDFSVAAGK
                     ITVFVGAYDLGRTLAVGGSGGSSATGSEDFVNNALGRGQAGALSAVKTDFGPWGGSMS
                     FNSNANWYFDPDARTDESFPGKYDFYSVAVHELAHVLGFGGSASFENLASSGVFTGSS
                     VQGLLGYAPPVTTDGHWARGLSYLGQEAAMGPDIGANERQHFTALDYAAMKDTGWQVS
                     PIPEAETWSMLLAGLSLLGWRLRSPRSNRDITSRA"
     gene            2841..4550
                     /gene="ybaL"
                     /locus_tag="VFI43_07375"
     CDS             2841..4550
                     /gene="ybaL"
                     /locus_tag="VFI43_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HET7061983.1"
                     /translation="MPHSISLITTIAVSFGLALLMGLIANRLKLPVLVGYLTAGVILG
                     SHTPGFVADMELSGQLAEIGVILLMFGVGLHFSLDDLLAVRRIALPGAVVQIAVATAL
                     GAAVSHMWGWNTGVAIVFGVALSTASTVVLLRALEQRGLLKSVNGAIAVGWLIVEDLA
                     MVLVLVLLPPLSGWLGGNPDAGSGAPDFSLLTTLLLTFGKVAVFIALMLVVGKRVFPW
                     LLWHVASTNSTELFILSVIAVAIGIAYGSAVLFGVSFALGAFFAGMVMRESDLSHRAA
                     QESLPLRDAFSVLFFVSVGMLFDPNVLVEQPIRVLATIGIIIIGKSVAAFFLVLLFRY
                     PLNTALTVSASLAQIGEFSFILAALGVSLGLLPVEAQSLIVAGAFISITLNPLVFRVI
                     EPAQAWIRSRSNLARILERSDDPLAELPTSVASSYVTSHVVLVGYGRVGRRIAEALTG
                     QGVPFVVAEQNRDLVEGLRKRGTHAVAGDAAEPAVLIQAHVARAAMLVIAVPDTLRAR
                     RMIEIARMLNPPIQVIVRTHNEEEAALLQKESEVNVFLGEHELALSMTRLVLEKIRTS
                     PTG"
     gene            4639..4992
                     /gene="arsC"
                     /locus_tag="VFI43_07380"
     CDS             4639..4992
                     /gene="arsC"
                     /locus_tag="VFI43_07380"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179252.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET7061984.1"
                     /translation="MNDRITIYQKPTCSKCRETLKLLRESGQEFDAINYYETPLTAVQ
                     LRELLDKLGLPPRAILRSEESLARSMKLGQRELSDDELIQIMVENPDLIQRPIVVRGD
                     QAELCRPPENVMKLL"
     gene            5173..5655
                     /locus_tag="VFI43_07385"
     CDS             5173..5655
                     /locus_tag="VFI43_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7061985.1"
                     /translation="MMFRELIRALFKTSYSWRKIGIARVREGFDWKKTVKLATFIALL
                     GLAGPGAVPQAQAEAECSGCICPGNPCKLCPLPPVKNGSAVSQEADTCRKIQEKVPPV
                     SKHTEPNEHYASLNRSIMECVRNGGDVLMNSRRSTEFPSKHYCKPYIPSSKADTAKGR
                     "
     gene            5678..7180
                     /locus_tag="VFI43_07390"
     CDS             5678..7180
                     /locus_tag="VFI43_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7061986.1"
                     /translation="MIARAEARHESVGETGSSSWLRRNPSSFLKLILLGFALVGLPLI
                     IALINSALSIDRLAEHSRKAVYQAAQVAHSSRALADEIAAMERAVRQTHILGDNALLE
                     GYFRAHDQFEITAASLLELSLHAEQKELLIKLQALEASIFEKLSIVRQSPDELRELIG
                     NFAALRDSARSFSYLGQSLIEREVGEMQDMAGYARFTVGWQLLALIPFAILLAFMFSL
                     RIARPIQQIDEAIRNMGQGKLSKAISVDGPQDLVYFGERLDWMRRRLLKLEEQKTRFL
                     QHVSHELKTPLTAMREGADLLAEGVAGELTEEQQRIANILHNNSLQLQRRIEDLLSYS
                     ALQNERAALIRHRANLRNILEAVLQDQNLAIMSKGLRIEVSCPELVVDCDGQKIKTIV
                     DNLLSNAVKFSPQRGRISIWANRIEESIVLNVLDAGAGIDEADRDKVFEPFYQGRQMH
                     NSPIRGTGLGLSIAREYALAHGGNIELIPQPAAGAHFRLTIPIRDPEGSS"
BASE COUNT         1597 a         1929 c         2098 g         1667 t
ORIGIN      
        1 ggcgctaaac ggacgctatt ttccgttgcg aagtgagctg cctgagtgtt cgatattgat
       61 aactcatcat agatggctct aatgaaatat ctactgcctt aaggttgttc gacatacgac
      121 tccaatgccg caggcccgcg accggatcgc gatgaccttt ctgcagccgc atgccagtat
      181 ccagaactgc gaggatattt ctaccaataa cgtctacaga aacgagacgc agcccaccaa
      241 gccatatcct cattgagcgc agcactattg cgggaattat tttccaaaaa atcgggaaga
      301 tggccggaag gcggttgtat acttcacaaa gcagagctgt tctcttgatg caatttgccg
      361 gcctgcagga attgcactac ttccccatct ggtcttgtcc aaacgtcgtt ttgctccacc
      421 tatactgcct ataatcatcc ggaataaata atgctgatcg gactgcccaa ggaaacaaaa
      481 gaccacgaaa cgcgggtcgg aatgaccccc agcacggtaa gagcgctgac acgcagcggg
      541 catgaggtac gggtacagcg gggggccggc gagggtagtt ttcttgcgga tgaagaatac
      601 gttgctgcag gagcggtaat tgttcctcag gctgaggacg cctgggcggc gcacatggta
      661 gtaaaggtca aggagcccac acccgccgaa tatcgttacc ttcatcaggg aatgattctt
      721 tttacttacc ttcacctggc ttcggataaa gcgctgacct gtgccctcgt cgatacccgc
      781 gttaccgcca ttgcttatga aaccgtacag accgaagcag ggaaactgcc gttgctgacg
      841 cccatgagcg aagttgcggg acgaatggcc gttctggttg gggcaacata tctgcaaaga
      901 acgcatggcg gtcgcggcgt actgctggga ggggtgccgg gagtcgaatc cgcgaatgtg
      961 gcgattctcg gcggcggcat cgtcggcacg aatgctgcac gcatcgcagt gggctgcggc
     1021 gcacaggtga ctctcctcga tgtgaatcat gatcgcctga aatacctgga tgacatatac
     1081 cggggtcagt tgcagacacg tttcagcgat gaatacaacc ttgaagacgc tgtgttcaat
     1141 gcggacctcg tgattggcgc ggtactgatt cccggtgggc gggcgccctg gctgattacg
     1201 gcaggaatgc tgccaaagat gaggaaaggt tcggtaattg tcgatgtggc ggtcgatcag
     1261 ggaggatgcg tcgaaacgac gcgtcccacg acgcacagca accccacgta cgagatcgat
     1321 ggcgtggtgc attactgcgt ggcgaatatg ccaagtgcag ttccccgtac cagtacattt
     1381 gctttgaata tacagacagc aatgtatacg gttcttcttg cgaatgaagg actcgatgcg
     1441 gtgcgaaaaa accgcgcgct tcaattcgga ttgaatacgt acggcggatc ggtgacctat
     1501 cctgccgtcg caaccgaatt cgggatgaaa tatactgaac cattggaggc tttggcaggg
     1561 ggcgattaaa aggggccctg aaggggtgat taacatcggg gtccaataaa atggaccgct
     1621 gaatattggc acaggagaga agcaagatga aaatcagata tttcccgttt tttgcggtat
     1681 tggtcgcccc atttttcacg gctgggcagg ccgctttagc caatgtgggt atcgctttcg
     1741 attatcgata tgacagttcg ggttttttca ccggcgcgaa cagcgggcgg caaaatttgc
     1801 tggagtccgc ggcatccgtg ttcgagaatc gttttcagga caatctgact gaaatcactt
     1861 catccggatc gaaccaattc accgccaagc ttttcgagcc agataacggc aagaacatca
     1921 gcattccgga tttcagcgtg gctgccggca aaataactgt cttcgtggga gcctatgatc
     1981 tcgggagaac gctcgcggtg ggtggatccg gaggttccag cgccacggga tcagaagatt
     2041 tcgtcaataa tgcccttggt cgcgggcagg ccggagcatt atccgcggtg aagacggact
     2101 ttgggccgtg gggtggctcg atgtctttca atagcaatgc aaactggtat ttcgatcccg
     2161 atgcccgcac cgatgaatcc tttcctggca aatacgattt ttattcagtg gctgtacatg
     2221 agttggccca tgtcctaggt tttggaggtt ccgcatcatt cgagaacctg gcctcaagcg
     2281 gcgtctttac cggttcgagc gtgcaaggtc tgctagggta cgctccgccg gtgaccaccg
     2341 acgggcactg ggcacggggg ctgagctatc tcgggcagga ggctgcgatg ggtcccgaca
     2401 taggagccaa tgagcggcag cattttaccg ccctcgatta tgctgccatg aaggataccg
     2461 gctggcaggt ttcgccaata cccgaggcgg aaacctggtc catgttgctt gccggactga
     2521 gtttgcttgg ttggcggctt cgttctccac gttcaaaccg ggacattacg agccgcgctt
     2581 gagccgcccc aggcggacca catgggctcg tctgctggag tgcgttataa tcgcacctca
     2641 ggatcatgct cagcgagaaa agctcggcac taatttttga actcatcgga ttgagaatac
     2701 cctcggaaat cactctttca tgacggcgaa acgccgtaac cagaagcact gccttcacaa
     2761 attccgcgtc cccgcactgg attgccccag aatattgggg gatgggacta cccctgccgc
     2821 acccgtagtt tcctccaccc atgcctcata gtatttccct gatcaccact attgccgtca
     2881 gtttcgggct agccctactg atggggttga ttgccaatcg gcttaagctt cccgtattgg
     2941 tgggatacct gactgctggc gtcatccttg gatcgcacac accgggcttt gtcgcggata
     3001 tggaactatc cgggcaactg gctgaaatcg gggtcatcct gctcatgttc ggcgttggac
     3061 ttcatttttc actggacgat ctgctggctg tgcggcgcat cgcattgccc ggcgccgtcg
     3121 tccagatcgc ggttgcgact gccctgggag cggctgtttc gcacatgtgg ggatggaata
     3181 caggggtcgc gatcgtgttt ggggtagccc tgtccacggc aagcaccgtc gtgctgcttc
     3241 gggcgctgga gcagcgtgga ttgctcaaat cggtaaacgg agcgatcgcg gttgggtggc
     3301 taattgttga agatcttgca atggtcctag tgcttgttct actcccgccc ctttcgggct
     3361 ggctcggcgg aaatccagac gcagggtccg gcgcacctga tttcagcctg ttgaccactc
     3421 tcctgcttac ctttggcaag gtagcggttt ttatcgctct catgctcgtg gtcggtaaac
     3481 gtgttttccc ctggctgctg tggcacgtgg ctagcaccaa ttcgacggaa ctcttcattt
     3541 tgagcgtgat agccgtggca attggcattg cctacggttc ggccgtgctc tttggcgttt
     3601 ccttcgctct cggtgctttt tttgccggaa tggtcatgag ggagtcggat ctgagccata
     3661 gggccgccca ggagtccctg cccctcaggg atgctttttc agtccttttt tttgtttccg
     3721 tcgggatgct ctttgatccg aacgtgctgg tcgaacagcc gatacgcgtt cttgccacta
     3781 tcgggatcat tatcatcgga aaatcggttg cggccttttt cctggtgctt ctttttcgct
     3841 atccgttgaa tactgcgctc acggtatcgg caagcctcgc gcagatcggg gaattttcct
     3901 ttattctggc tgcacttggc gtatccctcg gactcctccc ggtcgaggcg caaagcctta
     3961 tcgtcgcggg cgccttcata tcgatcacgc ttaatcccct ggttttccgc gtgatcgagc
     4021 cggcccaagc ctggatacgt tcacgctcca atctcgctcg catccttgag cgctcggacg
     4081 acccgcttgc agagttgcct acgtccgtcg catcaagcta tgtcacgagc cacgtcgtgc
     4141 tggtgggcta cggacgtgtg gggcgtcgta ttgccgaagc cctcacggga cagggggtcc
     4201 ctttcgttgt ggcggagcag aatcgcgacc tggtggaagg tttgcgcaag cggggcacac
     4261 acgccgtcgc gggcgacgct gccgagccgg cggtattgat acaggcgcac gttgctcgag
     4321 cggcgatgct ggtgattgcg gtacccgaca cattgcgtgc ccggcgcatg attgaaattg
     4381 cgcggatgct taatccgcct atccaggtta tcgttcgtac gcataatgag gaggaagcgg
     4441 ctctgttgca aaaggaaagc gaggtcaacg tatttttggg ggagcatgag ctcgcgctaa
     4501 gcatgacccg ccttgtactt gagaaaatac gcacctcgcc gactggctaa ggcataagga
     4561 tcccggggaa ataaagggaa cctgacggaa aaccagccgc cctaagcgac tgtgatttaa
     4621 ctcaggagga gaacagcaat gaatgacagg atcacgatct atcagaaacc gacatgcagc
     4681 aaatgcaggg aaacgctcaa gcttctcagg gagagcggtc aagagttcga tgcgatcaat
     4741 tattatgaaa ctcccctcac cgctgtccag ctccgggagt tgctcgataa actgggattg
     4801 ccgccgcggg cgatcctccg aagcgaggag tcgctcgcgc gcagcatgaa gctgggccag
     4861 cgggagcttt cggatgatga gttgatccag atcatggttg aaaaccctga cctgattcaa
     4921 cgcccgatcg tggttcgggg cgaccaggcc gagttatgcc gtccccctga gaatgtcatg
     4981 aaactgctct gagaatggaa aaacaaccct ttgagaacgg aaaagcagct acattcgttt
     5041 ccagccgaga tttcacttgc ggatattcgc taacaacggg atattggtat aaagtagctt
     5101 cgaagctgga agaggctgag ataggaaatc tgccgcgtgt tatgcagcca tgggaattga
     5161 tcaaggtaaa gaatgatgtt cagggaattg attcgggcac tgtttaagac ctcgtattcc
     5221 tggcgcaaga tcgggattgc gagggtacgc gagggatttg actggaaaaa aacggtcaaa
     5281 cttgcgacgt tcattgcgtt gcttggcctt gcaggtcccg gcgcagttcc tcaagcgcag
     5341 gctgaagccg aatgttccgg ctgcatttgc cctggtaatc cgtgcaagct ctgtccgttg
     5401 cctccggtca agaatgggag cgcagtttct caggaggcgg atacatgccg gaaaatccag
     5461 gagaaggttc cccccgtgtc caagcacacc gagcccaacg agcattacgc cagcctgaac
     5521 aggtccatca tggaatgcgt cagaaacggt ggggatgtac tgatgaactc tcgccggagt
     5581 acggaatttc cttccaagca ttattgcaag ccatacatcc cgtccagcaa ggcagacact
     5641 gccaaaggcc gctgaccaac aagaaccagg ggagaaaatg attgcacgcg cagaagcaag
     5701 gcacgagagc gtcggagaga cgggttcctc gtcgtggctg cgccgtaatc ccagttcttt
     5761 tctcaagctg attctgctgg gttttgccct ggtggggctg cccctgatca ttgcgcttat
     5821 caatagcgct ctttcaatag accggctggc cgagcacagc cgaaaagctg tgtatcaggc
     5881 tgcacaggtt gcgcatagca gtcgcgcctt ggctgatgaa atcgctgcca tggagcgcgc
     5941 tgtacggcaa acgcacatac tgggcgataa cgcactgctc gagggttatt ttcgcgctca
     6001 cgaccaattc gagatcacgg cagcaagcct gttggagctt tcgcttcacg cggaacagaa
     6061 ggaactcctg ataaaactcc aggcgctgga agcgtctatc ttcgaaaagc tctccatcgt
     6121 acgccagtcc cccgacgagc tgcgcgagtt gatcggtaat tttgccgcac ttcgggattc
     6181 cgcccgaagc ttctcttatc tcggccagtc gctgatcgag cgggaagtgg gcgagatgca
     6241 ggacatggcg ggatacgccc gtttcaccgt aggctggcag cttcttgccc tgattccgtt
     6301 tgcgatcctg cttgcgttca tgttttcgtt gcggatcgcg cgtcccattc agcaaattga
     6361 tgaggctata cgaaacatgg ggcagggcaa gctgtccaaa gccataagcg tggacggccc
     6421 tcaggatctg gtgtattttg gcgagcgcct cgactggatg cgtcgccggc tgttgaaact
     6481 ggaagagcag aaaacacgct tcctgcagca tgtttcccat gagctcaaaa ctccgctcac
     6541 agcaatgcgc gagggtgccg acctgcttgc cgaaggcgtg gcaggtgagt tgaccgaaga
     6601 gcagcagcgc atcgcaaata tcctgcacaa caacagtctc cagttgcaga ggcggatcga
     6661 ggatttactg agctacagcg ccttgcaaaa cgagagagcg gcgctgataa ggcatagggc
     6721 aaacctgagg aatatacttg aggcggtatt gcaggaccag aatctggcga tcatgagcaa
     6781 gggcttgaga atagaagttt cctgcccgga gctcgtggtg gattgtgatg gacaaaaaat
     6841 aaaaacaatc gtggacaacc tgttatcgaa tgctgtgaaa ttttcgcccc aacgcggccg
     6901 catcagcatc tgggcgaata ggatagaaga gtcgatcgta ctgaacgttc ttgacgccgg
     6961 ggccggaatc gatgaagcgg accgcgacaa ggtcttcgag cccttctacc agggacggca
     7021 aatgcataac agccctattc gcggcactgg tttagggctc tccatcgctc gtgaatatgc
     7081 cttggctcac ggcggcaata tcgaactcat cccgcagcct gcggccggtg ctcatttccg
     7141 cctgaccata ccgattcgtg atccggaagg ttcctcatga cccagtacta ccatgcgcgg
     7201 gtgattggcc ttgccattgc tctcgtatcc ctgattgggt gttccgcgat tcccgagcaa
     7261 cagccgtgcc agtgcccacc cgccgtcgag c
//
LOCUS       DASSHJ010000191        17383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_789952, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000191 DASSHJ010000000
VERSION     DASSHJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789952"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..2172)
                     /gene="fusA"
                     /locus_tag="VFI43_07395"
     CDS             complement(82..2172)
                     /gene="fusA"
                     /locus_tag="VFI43_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7061987.1"
                     /translation="MARQTPIERYRNIGIMAHIDAGKTTTTERILFYTGVSHKLGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMENNYPSHRINIIDTPGHVDFTIEVERS
                     LRVLDGACTVFCAVGGVQPQTETVWRQANKYKVPRLAFVNKMDRAGANFMRVYEQIQA
                     RLKAQPVPIQLPIGAEDKFEGVIDLVKMKAIYWDDSSQGMRFDEREIPADLLDDAKHW
                     REKMVETAAEANEELMNKYLEEGDLSVADIKQGLRIRTLSNEIVPMLCGSAFKNKGVQ
                     AMLDAVLDYLPSPKDIQAINGEKENGEPGERHAKDEEPFAGLAFKIATDPYVGQLIFF
                     RVYSGVVSSGDTVYNPVKGRKERIGRLLQMHANQREEIKEVRAGDIAAAVGLKEAITG
                     DTLCDPSEVITLERMTFPEPVIHVAVEPKTKLDQEKMGIALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEANVGAPQVAYREAIKKPVEIEGKFIKQSGG
                     RGQYGHVWLKMEPHESGKGFEFVDAIKGGTVPREYIPAVQKGLEETLPNGVLAGFPVV
                     DVKVTLFDGSYHDVDSNENAFKMAASIAFKDGMRKASPVLLEPMMSVEVETPADFMGN
                     VVGDLSSRRGMIQGMDDLPGLKVIRAEVPLSEMFGYATSLRSATQGRATYTMEFKHYS
                     EAPKNVAEAIINKK"
     gene            complement(2238..2708)
                     /gene="rpsG"
                     /locus_tag="VFI43_07400"
     CDS             complement(2238..2708)
                     /gene="rpsG"
                     /locus_tag="VFI43_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7061988.1"
                     /translation="MPRRREVPKREILPDPKYHNTEVAKFVNVLMTRGKKSVAERIIY
                     GAMEQIQKRTGKDPVEVFTQALSNVRPMVEVKSRRVGGANYQVPVEVRSIRRNALAMR
                     WLRDAARKRAEKSMGSRLAGELAEAAEGRGGAVKKREEVHRMAEANKAFAHYRF"
     gene            complement(2774..3151)
                     /gene="rpsL"
                     /locus_tag="VFI43_07405"
     CDS             complement(2774..3151)
                     /gene="rpsL"
                     /locus_tag="VFI43_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7061989.1"
                     /translation="MPTISQLVRKPRAARRVKSKVPALGNSPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQGRSKYGAKRPKAA"
     gene            complement(3432..4868)
                     /locus_tag="VFI43_07410"
     CDS             complement(3432..4868)
                     /locus_tag="VFI43_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7061990.1"
                     /translation="MKEWKAGVMPIDLDAIVAETKGRNFEIFEEHVNPVFVKVLRTLG
                     FNRTWVRGEGAYLWDDTGTQYLDFLTNWGVFNFGRRHPVIRGALQQVLDSEFPGWIGF
                     DAPPLAATLAQELVKRMPPGLDTVYFCNSGTEAIEAAIKFARAYTGRPSTAHLSKAFH
                     GLTMGSLSLNGEASFRRGFEPMLPGSSEVKLGDLEGLEAKLSRGDVAAFVFEPIQGKG
                     VNIASDEYLLGAQRLCRKHGTLMICDEVQSGMGRTGRFLASQWVPGLEPDVVCLSKAL
                     SGGYIPVGATITRRAVYDSVYDGMHRAIVHASTFGMGNMAMVAALASLKVLEEENLEQ
                     RALELGARFRNGIEAMVPRFEFLKGVRQRGLMIAIEFGQPDSMSLRAAWSMVNKMDEN
                     LFAQAIVLPLLDDHHILTQVAGHAMPVIKILPPLNISESDVDWFLGALEDVMMKLHKF
                     PGPAWEVLKKLGNHALTAKRREGRAAVS"
     gene            complement(4925..9145)
                     /gene="rpoC"
                     /locus_tag="VFI43_07415"
     CDS             complement(4925..9145)
                     /gene="rpoC"
                     /locus_tag="VFI43_07415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET7061991.1"
                     /translation="MKALLDLFKQVTQKEEFDSIKIGLASPDKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLQRCQLL
                     TDDDYRAKTEEYGDDFRASMGAEGIRDLLSNLNVRAEIDNLRREMGTTGSETKMKKIA
                     KRLKVLEAFNKSGIKPEWMVLAVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLEIMGIAS
                     TIKAAKREVENESPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMECRTLMMSTNNVLSPANGEPIIVPSQDIVL
                     GLYYTTREKVNARGEGMRFSNISEVSRAYESRIIELNARITVRIKEYEVKADGERCEK
                     ITRYDTTVGRALLSEILPAGLPFPLINKVLKKKEISRLINASFRRCGLRETVIFADKL
                     MYAGFSYATRAGISICLDDMLTPAQKDDIIGSAEREVQEIEVQYTSGLVTQGERYNKV
                     VDIWGRAGDQVAKAMMDQLGVEPVHDPVTGENKKDVNGKQLTQESFNSIYMMADSGAR
                     GSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALK
                     TANSGYLTRRLVDVTQDLVVTQDDCGTSNGVVMKALVEGGEVIEALRERILGRVVAND
                     IINPEHQTVLYPSGVLLDEKAVDMIESLGIDEVKVRTPLTCETRYGLCAKCYGRDLGR
                     GTPVNVGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVASQVESKSNGIVRYSPS
                     MRYVTNARNELVAISRSGEVVIYDDNGRERERHKAPYGATLLIRDGEVVRAGQVLAAW
                     DPHTRPIITEYSGKVRFENVEEGVTVAKQIDEVTGLSTLVVIDPKRRGVVQGKGLRPM
                     VKLFDEEDKEVKLAGSNLPVNITFQVGSVITVRDGQQVNVGEVLARIPQESSKTRDIT
                     GGLPRVAELFEARSPKDAGVLAEVTGIVSFGKDTKGKQRLIITDLDGVSHEYLIPKDK
                     HVTAHDGQVVNKGESIVDGPADPHDILRLLGIEALARYITDEVQDVYRLQGVKINDKH
                     IEVIVRQMLRRVQIVDPGDTRFIPGEQVERADMLAENEEVELQNKQPATYEYMLLGIT
                     KASLSTDSFISAASFQETTRVLTEAAIMGKKDDLRGLKENVIVGRLIPAGTGLTFHNA
                     RKKQRLLLNTEESHELREEDTGNQQFAL"
     gene            complement(9237..13310)
                     /gene="rpoB"
                     /locus_tag="VFI43_07420"
     CDS             complement(9237..13310)
                     /gene="rpoB"
                     /locus_tag="VFI43_07420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7061992.1"
                     /translation="MSYSFTEKKRIRKSFAKRASVLQIPFLLATQIDSYAAFLQAEVE
                     PNHRANQGLQAAFTSIFPIESHSKNARLDFVNYVLGVPPFDVKECQQRGLTYASPLRA
                     KVRLTIMDKEASKPTVKEVKEQEVYMGEIPLMTSTGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDYLYFRVDRRRKMPITVLLKAIG
                     YTPEQILADFFSFDTFHLTQEGIFFKLIPERLRGDIARFEIATKSGKVIVQKDKRITV
                     KHVREMQQAGIENLEVPEDFVLGRVLAHNVVDKETGEVLALANDEVTEALLTKLRQAN
                     IEQIQTIYTNDLDQGAYISQTLRIDETADQMAAQVAIYRMMRPGEPPTEEAVKVLFNG
                     LFYAPERYDLSVVGRMKFNRRIGKTQLDGPTTLSNEDITAVIKILVELRNGRGEVDDI
                     DHLGNRRVRSVGELAENQFRSGLVRVERAVKERLSQAESENLMPHDLINAKPVSAAVR
                     EFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSLALYARTNEYGFMETPYRKVKNGQVTDEIDYLSAIEEGQYVIAQA
                     NAQLDQNGRFTNEIVSCRHKNEFALSTPDRIEYMDVAPAQIVSVAASLIPFLEHDDAN
                     RALMGSNMQRQAVPCLRAEKSLVGTGIERVVAVDSGTAVQAVRGGAVDYVDASRLVVR
                     VHDAETRAGEVGVDIYNLTKYTRSNQNTNINQRPLVKVGDVIARGDVVADGASTDKGE
                     LALGQNMLVAFMPWNGFNFEDSILISERIVADDRFTSIHIEELSVVSRDTKLGTEEIT
                     GDISNLSEAQLSRLDASGIVHIGAEVEAGDVLVGKVTPKGETQLTPEEKLLRAIFGEK
                     ASDVKDTSLRVPSGISGTVIDVQVFTREGIERDKRAQQIIDDELKRYKKDLADQMRIV
                     EADAFKRIERLLIGKAATGGPKKLAKGTPITKEYLDSVDPHHWFDIRLAEEDASVQLE
                     QIEEGLSQKRKAFDMAFEEKKKKLTQGDELPPGVQKMVKVYVAVKRRLQPGDKMAGRH
                     GNKGVISKIVPVEDMPYMADGTPVDVVLNPLGVPSRMNVGQILETHLGWAAKGLGKKI
                     GDLMRAQASVAEIRAMLDKIYNTSGKPEDITSLDDAEVLHLAENLKDGVPFATPVFDG
                     ATEQEIKNMLELAGLPRSGQITLYDGRTGEAFDRPVTVGYMHVLKLHHLVDDKMHARS
                     TGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYTLQEMLTVKSDDVNGRTKVY
                     ESIVKGDHKIDAGMPESFNVLVKEIRSLAMDIDLERH"
     gene            complement(13484..13864)
                     /gene="rplL"
                     /locus_tag="VFI43_07425"
     CDS             complement(13484..13864)
                     /gene="rplL"
                     /locus_tag="VFI43_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET7061993.1"
                     /translation="MAVAKAEILDAIANMTVLELSQLIKDMEEKFGVSAAAAAVVSAA
                     PAAGAAAAPAEEQTEFTVVLTAVGESKVNVIKVVRAITGLGLKEAKDLVDGAPKPVKE
                     GIPKADAEAIQKQLTEAGATAEIK"
     gene            complement(13920..14444)
                     /gene="rplJ"
                     /locus_tag="VFI43_07430"
     CDS             complement(13920..14444)
                     /gene="rplJ"
                     /locus_tag="VFI43_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET7061994.1"
                     /translation="MGLNLEEKKAVVADVSAEVAKAQAIIIAEYRGLGVGNMTKLRAK
                     ARESGIYFRVLKNSLARRAVDGTPFSGLSDQMVGPLAYGIGSDPVAAAKVLYEFSKGN
                     DKFVIKAGAMPDLVMSGKDIASLAALPSRDELLSKLAGTMQAPIANFVRTLNEVPARF
                     VRGLAAVRDKREAV"
     gene            complement(14825..15517)
                     /gene="rplA"
                     /locus_tag="VFI43_07435"
     CDS             complement(14825..15517)
                     /gene="rplA"
                     /locus_tag="VFI43_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET7061995.1"
                     /translation="MGHVSKRVKSIRAKMDRSKFYPLAEAFRIIKETATAKFDESVDV
                     AVNLGIDAKKSDQAVRGSVVLPSGTGKTARVAVFAQGDKAKEALAAGADIVGFEDLAE
                     KIKGGDIDFDVAIASPDAMRIVGQLGQILGPRGLMPNPKVGTVTPDVAGAVRNAKAGQ
                     VQYRADKGGIVQCPIGRASFSTEALTQNLLALLDALNKSKPATSKGIYLRKISISSTM
                     GIGVRVDQTSMR"
     gene            complement(15519..15950)
                     /gene="rplK"
                     /locus_tag="VFI43_07440"
     CDS             complement(15519..15950)
                     /gene="rplK"
                     /locus_tag="VFI43_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET7061996.1"
                     /translation="MAKKITGYIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     ATQKMEVGLPVPVVITAYADKSFTFTMKTTPATVLIKKAAGVGKGSAKPHTDKIGRLT
                     RNQAEEIAKAKMPDLTAADMDAAVRTIAGSARSMGIEVEGI"
     gene            complement(16048..16581)
                     /gene="nusG"
                     /locus_tag="VFI43_07445"
     CDS             complement(16048..16581)
                     /gene="nusG"
                     /locus_tag="VFI43_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET7061997.1"
                     /translation="MDKKWYVVHAYSGFEKSVQRALKDRINRAGMQDKFGQILVPVEE
                     VIEMKAGQKNLSERKFFPGYVLVEMEMTDETWHLVKNTAKVTGFIGGSAMKPTPISDK
                     EVQNILHQIQEGVEKPKPKVLFEPGEAVRVKEGPFTDFHGNVEDVNYDKSKIRVSVSI
                     FGRPTPVELDFSQVEKA"
     gene            complement(16581..16925)
                     /gene="secE"
                     /locus_tag="VFI43_07450"
     CDS             complement(16581..16925)
                     /gene="secE"
                     /locus_tag="VFI43_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET7061998.1"
                     /translation="MDKIKLGFALLLLIAGIAGFYYLSDSAMVLRVISVLAGIVLAVV
                     MVWFTPEGKQFYAFSRESAEETRRVVWPSRKETMQTTGIVFAFVLLMAIFLWMVDAGL
                     LLAVKYLMGQEG"
     gene            complement(17098..17173)
                     /locus_tag="VFI43_07455"
     tRNA            complement(17098..17173)
                     /locus_tag="VFI43_07455"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17138..17140),aa:Trp,seq:cca)
     gene            complement(17266..>17383)
                     /gene="tuf"
                     /locus_tag="VFI43_07460"
     CDS             complement(17266..>17383)
                     /gene="tuf"
                     /locus_tag="VFI43_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7061999.1"
                     /translation="VSVTVNLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE"
BASE COUNT         3736 a         5193 c         4267 g         4187 t
ORIGIN      
        1 cgtgacctat cgtcccaaca ttcacatgcg gcttcttccg ctcaaatttg ctctttgcca
       61 taataatcct ttacctgtga cttatttctt gttgataatt gcttcggcga catttttggg
      121 cgcttcagaa taatgcttga attccatcgt ataagttgca cggccctggg tcgccgacct
      181 cagcgaagtg gcatagccga acatttccga cagcggaact tccgcacgta tcaccttcaa
      241 tccaggtaag tcgtccatgc cctgaatcat gccgcgacgg gaagaaagat cacccacgac
      301 attacccatg aaatcagcgg gggtctcgac ttccactgac atcattggct ccagcagcac
      361 tggactggcc ttgcgcatac cgtccttgaa ggcgatcgat gccgccatct tgaaagcatt
      421 ttcattggag tcgacatcat ggtatgaacc gtcgaacaag gtcaccttta catcaacgac
      481 ggggaagcct gccaatacgc cgttcggaag tgtttcttcc aaccctttct gaaccgcagg
      541 tatatattcg cgcggcaccg tgccgccctt gatcgcgtcg acaaactcaa accctttgcc
      601 ggattcgtgc ggttccatct tcagccagac atgaccatac tgcccgcgcc ccccggactg
      661 cttgataaat tttccttcga tctcgaccgg cttcttgatg gcttcgcggt aagcgacttg
      721 cggagcgccc acgttggcct ccactccgaa ctcccgcttc atccgatcta caatgatttc
      781 gagatgcaac tcacccatgc cggaaatgat cgtctgcccg gattcctcgt cggttcgcac
      841 gcggaacgaa gggtcttcct gcgctaacct gttcagcgcg atacccatct tttcctggtc
      901 aagctttgtt ttcggctcga cggcgacgtg aatcacgggc tctggaaagg tcatgcgttc
      961 cagagtaatg acttcgctgg gatcgcacaa cgtatctccg gtaatcgctt ccttcaagcc
     1021 gaccgccgcc gcaatgtcac ccgcacgcac ttccttgatt tcttcccttt ggttggcgtg
     1081 catttgcaag agacgcccga tgcgctcttt tcgtcccttg acagggttgt ataccgtatc
     1141 acccgaactc actacccccg aatacacacg gaaaaagata agctggccga cataggggtc
     1201 ggtcgctatc ttgaatgcca gtcccgcaaa aggctcctcg tccttcgcgt gccgttcgcc
     1261 gggctcgccg ttttcttttt caccattgat cgcctggata tccttcggag aaggcaagta
     1321 gtccagcaca gcgtcaagca ttgcctgaac gcctttgttc ttgaatgccg atccgcagag
     1381 catgggcacg atctcgttgc tgagcgtacg gattcgcaat ccctgcttga tatcagcgac
     1441 tgacaaatcc ccctcttcga gatacttgtt catcaattct tcattcgctt ccgcggcggt
     1501 ttccaccatt ttctcccgcc agtgtttcgc atcgtccagc agatcagcgg gaatttcacg
     1561 ctcgtcaaac ctcatcccct ggctcgagtc gtcccagtaa atggctttca tcttgacgag
     1621 atcaatgacg ccctcgaatt tatcctcggc accaatgggc aactgaatag gtacgggttg
     1681 cgctttcagg cgagcctgta tctgctcgta cacgcgcatg aagttggcgc ccgctcgatc
     1741 catcttgttg acgaaagcca ggcgcggaac cttgtacttg ttagcctggc gccagaccgt
     1801 ttccgtctgg ggctgcacgc cccccacagc gcagaatacc gtgcaggccc catcaaggac
     1861 gcgcaacgac cgctcgacct cgatagtgaa gtctacgtga cctggagtat caataatgtt
     1921 aatgcgatga gacggatagt tgttttccat ccccttccag aagcaggtgg tcgcagcgga
     1981 agtgatcgtg attccacgtt cctgttcctg ctccatccag tccatggtag cggcgccatc
     2041 atggacttcg cctagcttgt gcgatacccc ggtatagaac aatatccgct cagtcgtcgt
     2101 ggttttaccg gcatcaatat gcgccataat gccgatgttc cgataacgct cgatcggtgt
     2161 ctgtcttgcc acagtgttta aaccctagtt gaaatgatca gtaaagtaat gaatgagtcc
     2221 gtccggcgtt gctgccgtta gaatcggtaa tgagcgaatg ccttattggc ctccgccatc
     2281 cggtgaacct cttcgcgttt ttttacggcg cctccgcggc cttcggctgc ttccgcgagt
     2341 tcacccgcca agcgggatcc catcgacttt tcagcacgct ttcgcgccgc atcgcgcagc
     2401 caccgcatag ccagtgcatt cctgcgaatc gatctcacct ctaccggaac ctggtaattc
     2461 gcaccgccga cccgtcggct ttttacttcc accattggac ggacattgga taatgcctgc
     2521 gtaaatacct ccaccggatc tttacccgtc cttttctgaa tctgctccat cgccccgtag
     2581 atgatccgct cagccaccga cttcttacct cgcgtcatca agacattaac gaatttcgcg
     2641 acctcggtat tgtgatactt gggatcaggc aggatttcgc gttttggaac ttctctccgt
     2701 cttggcattt tctaaccttg aaatctgatt aaaaccgcat gcttaaagca atgcggcgct
     2761 ccatgttcag tctttaggct gctttcggcc gttttgcacc gtattttgag cggccttgtt
     2821 tgcgatcctt cactcccgcg gtatccaggc tgccgcgaac ggtatggtag cgaacaccgg
     2881 gaagatcttt gacgcgcccc ccccgaatca ataccacaga gtgctcctgc aggttatgac
     2941 cctctccacc gatataactc gacacttcga aaccattcgt tagccgtacc cgagcgactt
     3001 ttctcaacgc ggaattcggc ttttttggag tggtagtgta gacgcgcgtg cagacacccc
     3061 gcttttgagg gctgttgccc aacgccggaa ccttgctttt cactcgcctt gcggcacgag
     3121 gtttacgtac taactgactg atcgtcggca tgatattcct tcaaaaacag gagagagatg
     3181 ccctcctgct gccatggata cggaatttcg ccgcatcgga tgcgtatccg ggggcgatga
     3241 aaaaagactg cgaattttag ggggtttgcc tgaacgtgtc aagacaggat ttcgtcaggc
     3301 aaatgaaccg cgagcccaag tgggaccgct cccggagata aataaactgt tcttccctga
     3361 aggggcgcca ccacgagcca aactgcgggg atcaaaaggt cggaaccccc actcctgatg
     3421 tgaattaaaa actagctgac cgcagcgcgg ccctccctcc gctttgcggt caaggcgtgg
     3481 ttacccagtt tcttcaagac ctcccacgca gggcccggga acttgtgcag tttcatcatc
     3541 acgtcctcaa gcgctccaag aaaccagtcc acatcggatt cgctgatatt caacggtgga
     3601 agtattttta ttaccggcat cgcatggcca gcaacctggg taagaatatg atgatcatca
     3661 aggagcggaa gaacgatggc ctgagcgaaa aggttctcat ccatcttgtt taccatgctc
     3721 cacgctgctc taagcgacat cgaatccggc tgaccgaatt cgatcgcaat cataaggcct
     3781 cgctgacgca cacctttaag aaattcaaag cgaggcacca ttgcttcgat accatttcta
     3841 aaacgagcgc cgagttccag cgcgcgctgc tcaaggtttt cctcttcaag gaccttgagc
     3901 gaggcaagtg ccgccaccat cgccatatta cccattccaa aggtggaggc atgaacgatc
     3961 gcgcgatgca ttccatcata gacactgtcg taaaccgcac ggcgagtaat cgttgcgcca
     4021 acgggaatat atcccccgga tagcgctttg gaaagacaga ccacatcggg ctcaagccca
     4081 gggacccact ggctcgcaag gaaacgtccg gtgcgcccca ttcccgactg cacttcgtcg
     4141 catatcatca aagtgccatg ctttctgcaa agtcgctgag caccgagaag atattcatcg
     4201 ctcgcaatat tgacaccctt tccttgaatg ggctcaaaga caaaggccgc gacgtcgccc
     4261 ctggaaagct ttgcttcgag gccttcgaga tcccccagct tgacctccga actacctgga
     4321 agcatgggtt cgaaaccgcg gcggaagctt gcttcgccat tcagggatag cgaccccatg
     4381 gtcaggccat ggaaggcttt cgaaagatga gccgtcgagg gacgtcccgt ataggctcgc
     4441 gcaaacttga tcgcagcttc gatcgcctcg gtacctgaat tacagaaata gacggtatcc
     4501 aggcccggag gcatgcgctt caccagttcc tgcgccaatg tcgcagcaag gggaggcgcg
     4561 tcgaagccga tccacccggg aaactccgag tccagtacct gctgcaaagc gccccgaatg
     4621 acaggatgac gccgaccaaa gttgaagacc ccccagttgg tcaagaaatc gagatactgt
     4681 gtccccgtgt catcccagag atacgcaccc tcgcctcgca cccaggtacg gttgaaaccg
     4741 agcgtgcgca gcaccttgac gaaaacggga ttgacgtgct cttcgaaaat ttcgaaattt
     4801 ctacccttcg tttccgcaac tattgcatcg agatcaattg gcataacccc ggccttccat
     4861 tccttcattt acctccacga aaaccaacac catcagtctc aagccgggcc tcgaccggcc
     4921 atgcttacaa cgcgaattgc tgattccccg tgtcttcttc acgcaactca tgactttcct
     4981 cagtattcag taacagcctc tgtttcttgc gggcattgtg gaaggtcagg ccggtccccg
     5041 caggtattag ccgtccgaca ataacgttct ctttaagtcc gcgcaagtcg tccttcttgc
     5101 ccatgatcgc agcctcggtc aggactcgcg tggtctcctg gaaggatgca gccgaaataa
     5161 aggaatcggt ggaaagcgac gccttggtaa taccaagcag catgtattca taggtggcag
     5221 gctgcttgtt ctgcaattcg acctcttcgt tttcggccag catgtcggca cgttcaacct
     5281 gttcaccggg aataaaccgc gtatcaccgg gatcgacgat ctggacccgg cgcaacattt
     5341 gccgcacaat gacctcgatg tgcttgtcgt tgatcttgac gccctgcaga cgataaacgt
     5401 cctgaacctc gtccgtaata taacgggcaa gcgcttctat acccagcaac cgcaaaatgt
     5461 catgcggatc ggcagggcca tcgacgatgc tctctccctt gttcaccacc tgaccatcgt
     5521 gagccgtgac atgcttgtcc ttgggtatca ggtactcatg cgagacaccg tcgagatcag
     5581 taatgatcag acgctgcttg cccttggtat ccttcccgaa cgatacgatt ccggtcacct
     5641 ctgcgagtac gcccgcatcc tttggtgagc gcgcctcgaa cagttccgcc acacgaggca
     5701 agccgccagt aatatcccgg gtcttcgagc tttcctgggg aatccgggcc agaacctcgc
     5761 caacattgac ttgctgcccg tctcgcacgg taatgacgga accgacctgg aaggtgatat
     5821 tgacgggcag attgctgccg gcaagcttca cttccttgtc ctcttcatcg aacagcttca
     5881 ccatcggacg cagacccttg ccctgaacaa caccgcgtcg tttcggatcg atcacgacaa
     5941 gcgtggagag gccggtcact tcgtcaatct gtttggcgac ggtaacgcct tcctcaacgt
     6001 tttcgaaacg gaccttgccg gaatattccg tgatgatggg gcgggtatgc ggatcccatg
     6061 cggccaatac ctgtccggcc ctcacgactt ccccatcacg gatcagcagt gttgcaccgt
     6121 aaggcgcctt gtgccgttca cgttcgcggc cattgtcatc gtatatgacg acctcgccgc
     6181 tccgggagat tgcaaccagc tcgttcctcg cattggtgac ataacgcatc gagggcgagt
     6241 agcgaacgat cccgttggac ttgctctcca cctggctcgc caccgctgtc ctcgaggccg
     6301 caccgccgat gtgaaatgtg cgcatggtaa gctgagtgcc cggctcgccg atcgattgcg
     6361 ccgcaatgac tcccactgcc tctcccacat tcacgggcgt tcctcgtccg aggtcacggc
     6421 cataacattt tgcgcacaag ccatagcggg tttcgcaggt caacggggtg cgtactttta
     6481 cttcatctat cccgagagac tcgatcatat caacggcttt ttcatccagc aaaaccccag
     6541 acgggtacaa aaccgtctgg tgctccggat tgatgatgtc gttcgcaacc actcttccca
     6601 aaatgcgttc gcgcagcgcc tcgatgactt cacctccttc cacgagggcc ttcatgacca
     6661 cgccattgct cgtaccgcaa tcgtcctggg taaccaccag atcctgggtg acatcaacca
     6721 ggcgacgggt aagatatccg gaattcgcag ttttgagcgc cgtatcagca agacctttcc
     6781 gtgcgccatg ggtcgagatg aagtattgca gcacgttcag accttcgcgg aaatttgcag
     6841 tgatcggcgt ctcgatgatt gagccatcgg gctttgccat gagaccccgc attccggcca
     6901 attgccgaat ctgagccgca gagccccggg cgccggaatc agccatcatg taaatcgaat
     6961 tgaacgattc ctgggtcaac tgctttccat ttacgtcttt tttgttttca ccggtcacag
     7021 gatcatggac gggttcgacg ccgagctggt ccatcatcgc cttggctacc tgatcgccgg
     7081 cgcgtcccca tatatccaca accttgttat agcgttcgcc ctgcgtcacg aggcccgatg
     7141 tgtactgaac ctcgatctcc tgcacttccc tttcagccga accgatgatg tcatccttct
     7201 gcgcgggcgt cagcatatcg tccaggcaaa tcgatattcc agctcgagta gcatagctga
     7261 agccagcgta catcaacttg tccgcgaaga tgactgtctc gcgcagaccg cagcggcgga
     7321 agctcgcatt gatcagccgt gagatttcct tcttcttgag tactttgttg ataagcggga
     7381 acggaagccc ggccggcaat atttcagaca gcagagcgcg acccaccgtc gtatcataac
     7441 gcgtgatttt ttcgcaacgc tccccgtcag ccttgacttc atattccttg atccgcaccg
     7501 tgatccgcgc gttcagttcg ataattcggc tttcataagc acgcgacact tcacttatgt
     7561 tggaaaagcg catgccttcg ccgcgagcgt tcactttctc ccgcgtcgtg taatacaggc
     7621 ccagtacgat atcctgtgac ggcacaataa tgggttcacc gtttgccggt gacagaacat
     7681 tattagtgga catcatgagc gtgcggcatt ccatttgcgc ttcgagcgaa agcggcacgt
     7741 gaacggccat ttggtcgcca tcgaagtcgg cgttgaacgc agcgcaaaca aggggatgca
     7801 gctggatggc cttcccttcg ataaggacag gctcaaacgc ctgtatgccc aaacggtgca
     7861 aagtcggggc gcggttcagc atcaccgggt gctcgcgaat cacctcttca aggatatccc
     7921 acacgacagg actttcgttc tcaacttcac gcttcgcggc ctttatggtg ctcgcaatcc
     7981 ccatgatttc gagcttgttg aagatgaacg gcttgaacag ctccagtgcc attttctttg
     8041 gcaggccgca ctggtgaagc tttagctgcg gacctacgac aatgacggag cgaccggaat
     8101 aatccacgcg cttgcccagc aggttttggc gaaaacggcc acccttgccc ttgatcatgt
     8161 ccgcgagcga cttcaatggc ctcttgttgg cgccggtcat ggccttgccg cggcgtccat
     8221 tatccagcag cgaatccacc gcctcctgca gcatccgctt ttcgttacgc acgattattt
     8281 ctggagcttt aagctcgaga agccgcttca accggttatt tcgattgatg acacggcggt
     8341 acaaatcgtt cagatcggac gtcgcaaaac ggccgccgtc cagcggcacg agagggcgaa
     8401 gctcgggcgg cagtacgggc agcactgcca ataccatcca ttccggcttg atgccagact
     8461 tgttgaaagc ttccagcacc ttcaaccgct tggcgatctt cttcatcttc gtttcggaac
     8521 ctgttgtccc catctcacgc cgaagattat cgatttccgc gcgaacattc agattgctca
     8581 gcaaatcacg gatgccttct gcgcccatgc tcgcgcggaa atcgtcgccg tactcttccg
     8641 ttttcgcgcg atagtcatcg tccgtcagca actggcagcg ctgcagcggg gtcattccag
     8701 gatcggttac gacatatgcc tcgaagtaga gcactctttc gatgtcacgc aaggtcatat
     8761 ccagtaccat gcccaagcgc gaaggcagcg atttcagaaa ccagatgtga gcgaccggcg
     8821 aggccaactc gatgtgcccc atgcgctcgc gccttacctt tgacaaggtc acttccacgc
     8881 cgcacttttc gcagatgacg cccctatgct tcaggcgctt gtattttccg cagagacatt
     8941 cgtaatcttt taccggtcca aaaattttcg cgcagaagag gccgtcccgc tccggtttga
     9001 acgtgcggta attaatggtc tcgggtttct ttacttcgcc atacgaccag gaacgtatct
     9061 tatccggcga agccaggccg atcttgatcg agtcgaactc ttctttctgt gtaacctgtt
     9121 tgaataaatc aagcagtgct ttcatttgtc actcctgttg atcaggggtc gatatgtaaa
     9181 aaatgatgcg ggccgggaag atcgtgcgca aacctccatg tctgcccggc gaattgctaa
     9241 tgccgctcaa gatcaatatc catcgccaag gaacgaattt ccttgaccag tacgttgaat
     9301 gactccggca tgcccgcatc gatcttgtga tcgcccttta caatgctttc gtatactttt
     9361 gtccgtccgt tcacgtcgtc ggacttgacg gtcaacattt cctgcaaggt atatgacgcc
     9421 ccatatgctt cgagcgccca tacttccatc tcgccaaacc gttgtccgcc aaactgcgcc
     9481 ttgccgccga gcggctgctg ggtgaccagg ctgtaaggac cggttgagcg cgcatgcatc
     9541 ttgtcgtcca cgagatggtg cagcttcagc acatgcatgt accctacggt gacgggacga
     9601 tcgaaagcct cgcccgtcct tccgtcgtaa agtgttatct gtcccgatcg gggcaggccg
     9661 gcaagctcca gcatattttt tatttcctgt tcggttgcac catcgaatac cggagttgca
     9721 aagggcacgc catccttgag gttctccgca agatgcagca cttctgcatc atcgagcgaa
     9781 gtgatgtctt ccggcttccc gctcgtgttg tagatcttgt cgagcatcgc gcgaatctcc
     9841 gcaacgctcg cctgggcacg catcagatca cctatcttct tgcccagtcc tttcgctgcc
     9901 cacccgagat gggtttcgag aatctggccg acgttcatgc gcgagggaac tcccagcgga
     9961 ttcagcacca catcgacagg ggtcccgtcc gccatgtatg gcatgtcctc gaccgggaca
    10021 atcttggata tgacgccttt gttgccatgg cggccggcca tcttgtcccc tggctgcaag
    10081 cggcgtttta ccgccacata aaccttgacc attttctgta cgcccggcgg caattcatcg
    10141 ccctgcgtca gcttcttttt cttttcctca aaggccatat caaaggcctt gcgcttctgc
    10201 gagagacctt cctcgatctg ctcgagctgg acgctagcat cctcctcggc aagccggatg
    10261 tcgaaccagt gatgaggatc cacgctatcc agatactcct tcgtgattgg tgtacctttg
    10321 gcgagctttt tcggtccgcc agtggccgct ttgcctataa gcaaacgctc gatacgcttg
    10381 aacgcgtccg cctccacgat ccgcatctga tcggcgagat ccttcttgta ccgcttgagc
    10441 tcgtcatcga tgatctgttg ggcgcgcttg tcgcgctcga ttccttctct tgtgaacacc
    10501 tggacatcga tgaccgttcc ggatattccg gagggaaccc gaagagacgt gtccttcacg
    10561 tccgacgctt tctcgccgaa aatggcgcgt aacagctttt cctccggggt cagctgagtc
    10621 tcgcctttcg gcgtgacctt gccaaccagc acgtcgcctg cttccacttc cgcgccgata
    10681 tgtacaatgc ccgaggcatc cagccgggag agttgcgctt ccgataagtt ggagatatca
    10741 cctgtgattt cttccgttcc gagcttggta tcgcgactca ccacggacag ctcttcaatg
    10801 tggattgatg taaaacgatc atccgccaca atgcgctccg aaatgagaat cgaatcctcg
    10861 aagttgaagc cattccatgg catgaaggca accagcatgt tctgccccaa cgccagctcg
    10921 cctttatcgg ttgacgcgcc atccgcaacc acatccccgc gggctatcac atccccgacc
    10981 ttcacaagcg gcctctgatt gatattggtg ttctggttgg agcgggtgta cttggtcagg
    11041 ttgtaaatgt ccacacccac ttcgccagca cgtgtttccg catcatgcac acgcacgacg
    11101 aggcggctgg catccacgta atcgaccgcg ccacctctca ctgcctgaac cgctgttcca
    11161 gagtccactg ccacgacacg ctcgataccc gtgcccacca gggacttttc agcccgcagg
    11221 caaggtacag cctggcgctg catgttggaa cccatcaatg cgcgattcgc gtcatcgtgc
    11281 tcaaggaacg gaatcagcga agccgccact gacacaatct gcgcgggtgc aacgtccata
    11341 tactcgatcc gatccggggt cgacagcgca aactcattct tgtgccggca ggaaacaatt
    11401 tcattggtga atctgccgtt ctggtcaagt tgcgcattgg cctgcgcgat cacatactgg
    11461 ccttcctcga tggccgacaa ataatcgatc tcatccgtca cctgaccgtt tttcactttc
    11521 cggtaaggtg tctccatgaa accatactcg ttggtgcgcg cgtacagcgc gagcgagttg
    11581 atcagtccga tattggggcc ttcaggcgtt tcgatcgggc atacccgccc gtagtgcgtc
    11641 gggtgaacat cgcgcacttc gaatcccgct cgctcgcgcg tcaatcctcc cggccccagc
    11701 gccgagaccc ggcgcttgtg ggtaatctcg gacaatggat tggtctgatc catgaactgc
    11761 gacaactggc tggaaccaaa aaattcacgc acggcagctg ataccggctt cgcgttgatc
    11821 agatcgtggg gcatcaggtt ttccgactca gcctgactca gtcgttcctt gactgcccgc
    11881 tccaccctca ccaaccccga gcggaactgg ttctccgcca gttcgccgac cgagcgcacg
    11941 cggcgatttc cgagatgatc gatatcgtcg acctctcccc ggccgttgcg gagctcgacg
    12001 agtatcttga taaccgcagt gatgtcttcg ttcgaaagcg tggtaggacc atcgagttgc
    12061 gttttgccga tgcggcggtt gaatttcatg cgccctacaa cggaaagatc atagcgttca
    12121 ggcgcgtaaa atagcccgtt gaatagtacc ttgaccgctt cctcggtagg gggttcccct
    12181 ggccgcatca ttcgatagat cgccacctgc gccgccattt gatcagcggt ctcatcgatc
    12241 ctcaaggtct gggatatgta tgcgccctgg tcgagatcat tcgtataaat agtttgaatc
    12301 tgctcgatat ttgcctggcg caacttggtc agcagggcct cggtaacctc gtcattggca
    12361 agagccaata cctcgccggt ttccttgtcc accacattgt gcgccaatac gcggcccaac
    12421 acgaaatctt caggcacctc aaggttttcg atgcccgcct gctgcatttc gcgaacgtgc
    12481 ttgacggtta tacgcttgtc cttttgaaca ataaccttcc cgcttttcgt agcaatctcg
    12541 aaccgtgcga tgtctccgcg caaccgctca ggaatcaact tgaagaaaat accttcctgg
    12601 gtcagatgaa acgtatcgaa cgaaaagaaa tccgccagaa tttgttccgg cgtgtaaccg
    12661 atcgccttca gcagaacggt aatcggcatc ttgcggcgcc tgtcgacgcg gaagtagaga
    12721 tagtccttgg gatcgaattc aaaatcgagc caggaacccc ggtacggaat gattcgcgca
    12781 gaaaaaagca gtttcccgga tgaatgggtt tttccacggt catgctcgaa gaacacgcct
    12841 ggcgaacgat gcaattggga tacgatgacg cgctccgtgc cgtttatgac gaacgagccc
    12901 gtgctggtca tgagcgggat ttcgcccatg tacacttcct gttccttgac ttccttcaca
    12961 gtgggtttgg aagcctcctt gtccatgata gtcagccgga cctttgccct tagtggcgat
    13021 gcataggtaa ggccacgctg ctggcattct tttacgtcaa acggcggaac gcccaataca
    13081 taattgacga aatcgagacg cgcattcttg gaatggctct cgatcgggaa aatcgacgtg
    13141 aacgcagcct gcagcccttg gttggcccga tgattgggct caacttccgc ttgcaggaaa
    13201 gctgcgtaag agtcaatttg cgtagcgaga agaaaaggaa tctgcaaaac gctggcgcgt
    13261 ttggcaaaac ttttgcggat acgttttttc tcggtaaacg aatagctcat ggaatctccg
    13321 gggaggtcag aggacggatg aacagaaaat ggaagcagag tacctgattt tggctaccgt
    13381 gctgcgtgtg ctaccgcaca ttatgcggga ttccaaacgt ttaaaacgcc atggcaggac
    13441 ggcgcgcgtt tcgcgccgtc ctgccaggtt tgttgcatga tgcttacttg atttcagcag
    13501 tcgcacccgc ctcggtcagc tgtttctgga tcgcttcagc gtcggccttg ggaatacctt
    13561 ccttcaccgg cttgggcgcg ccatccacca gatcttttgc ctctttcaga ccgagacccg
    13621 tgatcgcgcg cacaaccttg atcacattaa ccttgctttc gcctaccgcg gtcagcacta
    13681 cggtgaattc cgtttgctct tccgcagggg cagcagcggc gcctgccgca ggagccgctg
    13741 agacaacagc ggcagcagcg gcggaaacac cgaacttctc ttccatatcc ttgatcagct
    13801 gggaaagctc cagcactgtc atgtttgcaa ttgcgtccag aatttctgct ttggctacag
    13861 ccatgttttt ctcctgataa atttaagcta tgatcgtgtt aggttgccta aggataaggc
    13921 tagacggctt ccctcttgtc ccgaacggcg gcgagacccc gtacgaaacg tgccggaacc
    13981 tcgttgagtg tccgaacgaa attggcgatg ggcgcttgca tggtgcctgc aagcttcgaa
    14041 agtagctcat cgcggcttgg caacgcggca agacttgcaa tgtccttgcc ggacatgacc
    14101 aggtcgggca ttgctcccgc cttgattaca aacttatcgt tgcctttcga aaactcgtac
    14161 agcacctttg ccgcggcaac gggatcgctt ccgatgccgt aggccagggg tcccaccatc
    14221 tggtcggaaa gtccactaaa tggcgttccg tctactgcgc gccgcgcaag agagtttttc
    14281 agcacacgaa aatatattcc cgattcacgt gccttcgcac gcaatttggt catatttcca
    14341 acgcctagtc cgcgatattc ggcgataatg atagcctgag cttttgccac ctcggcgctg
    14401 acatccgcca ccaccgcttt tttctcttct aaatttaaac ccaaggttca ttctccagtt
    14461 aaaatagata ccgagcttag aaagtccctg cgtcagggca accgcaatca gctcaatatc
    14521 cgaaccggcg acctaaaatc aggagatgaa atctttcctg ttctgggagc gccatctgcg
    14581 ttgggcgtgg aacaaatcac gaagaaatgc ttgagtcttc gcttccgttc cataattaag
    14641 cgcctgggtc cgtctgctca atcgcttttc agcaaggctt gagcgtaccc gaacccgtta
    14701 accccaacgg tctttgataa cccgccgaac cggattattg gattatttcc ttgcttcccg
    14761 tccgacggcc caaagtctta caaaccgtat aaccgtataa ccgtataaaa ctgtattctt
    14821 gacctcaccg catgctggtc tggtcgaccc tgactcctat tcccatcgtg ctggaaatcg
    14881 aaatctttct gagataaatt cctttggatg ttgcgggctt ggacttgttg agcgcgtcca
    14941 gcaacgcaag caggttctgg gtcaatgctt cggtgctgaa ggacgcacgt ccgatcgggc
    15001 attgaacaat acccccctta tcggcccggt actgaacctg accggccttc gcatttctta
    15061 ccgcacccgc aacgtcgggc gtcacggtgc ctaccttggg gttaggcatc agtccgcgag
    15121 gtccaagaat ctgccccaac tggccgacga tgcgcattgc gtcgggactg gcgatggcaa
    15181 catcgaaatc tatgtcaccc cccttgatct tttccgcaag atcttcaaac ccgacgatgt
    15241 cagcacccgc cgccagcgcc tctttggctt tgtcgccttg cgcaaatacg gccacacgag
    15301 cagtcttgcc cgtgccggaa ggcaatacta cggaacctcg aaccgcctgg tctgattttt
    15361 ttgcatcaat accgagattg acagccacgt cgacggattc gtcaaacttg gcagtcgccg
    15421 tctccttgat aattctgaac gcttcagcca aagggtagaa tttgcttcga tccatttttg
    15481 ccctgatcga ttttactcgc ttggatacgt gtcccacctc agattccctc cacctcaata
    15541 cccatgctgc gcgcgctacc tgcgatggtg cgaactgccg catccatgtc cgcagccgtg
    15601 agatcgggca ttttggcctt ggcaatttct tccgcctgat tacgtgtcaa acggcctatc
    15661 ttgtccgtgt ggggcttggc gctccccttg cctactcccg ctgccttctt gatcagcacc
    15721 gtggcgggcg tggttttcat cgtgaaggtg aagctcttgt ccgcataagc ggtgataacc
    15781 accgggaccg gcagcccaac ctccattttc tgcgtagcgg cgttaaacgc cttgcagaac
    15841 tccatgatat tgagtcctcg ttgcccgaga gcgggtccta tgggaggact aggatttgct
    15901 tttcctgcag gtacctgcaa cttgatataa ccggttattt tctttgccac agaaattctc
    15961 ctattgggtg caagcgaatg taggttctcc ccattaaaca atcaacaggc ggtgttccct
    16021 atcgaacacg gcctgcactt cctgctttca ggccttctcc acctggctga aatccagttc
    16081 caccggagtt gggcgcccga aaatggaaac ggaaaccctg attttgcttt tgtcataatt
    16141 gacatcttca acattcccat ggaaatcagt aaatggccct tccttgacac gaacagcctc
    16201 cccaggctcg aacaggacct tgggcttcgg cttctccacg ccctcctgga tctgatgaag
    16261 gatgttctgc acctctttat cgctgatagg cgttggcttc atcgccgaac cgccgataaa
    16321 acccgtaacc ttggccgtgt tcttgaccag atgccacgtt tcgtcggtca tttccatctc
    16381 gaccagtacg taaccaggaa aaaacttacg ctcgctgaga ttcttctgac cggccttcat
    16441 ttcgatgacc tcttccacgg gaaccagaat ctggccgaat ttatcctgca tgccggcgcg
    16501 attgatgcga tccttcaaag cgcgctgcac actcttctcg aacccggagt aggcatgaac
    16561 cacgtaccat ttcttatcca ttacccttcc tgacccatga gatatttcac cgcgagcaac
    16621 aatccggcgt ctaccatcca gagaaatatg gccatcagca aaacaaaagc aaacacgatg
    16681 cccgtcgtct gcatcgtctc tttgcggctt ggccagacaa cgcgacgcgt ttcttccgct
    16741 gactccctgc tgaacgcata aaactgttta ccctcggggg taaaccatac catcactacc
    16801 gccagaacga ttcccgccaa aacggaaatc acgcgaagaa ccatggcgct gtcgctcagg
    16861 taataaaagc ccgcaatacc tgcgatcagc agcagaagcg cgaatcccaa cttgatctta
    16921 tccactaccc tatcccactc tgccgttaaa gttgtcagtc aacgaagcga tgcgtttact
    16981 gagtacaaat tacaatttca gcgcatgtat ctagcatatc gaggggaggc gtggcggcat
    17041 atcttgcttg cgttgcaaag caagcagaac tgaaggtttt caatgaaact ccttgtttgg
    17101 caggccagga gggcatcgaa cccccaacct tcggttttgg agaccgacgc tctgccaatt
    17161 gagctactgg cctgtattcc tgttgaggaa aagctgatgt aatgagattc actcaagtat
    17221 aacttgggga acatccagcc gggagacttc aagcccgccc gtcaactact cgataatctt
    17281 tgcgacgacg cctgcgccga cggttcttcc gccttcgcgg atggcaaagc gcagaccctc
    17341 ttccatcgca atgggtgcaa tcaggttgac cgtcaccgat acg
//
LOCUS       DASSHJ010000192         6865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1306292,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000192 DASSHJ010000000
VERSION     DASSHJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306292"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /gene="ipk"
                     /locus_tag="VFI43_07465"
     CDS             complement(<1..293)
                     /gene="ipk"
                     /locus_tag="VFI43_07465"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030480.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7062000.1"
                     /translation="MSEDNCLICPAPAKLNLFLHIVGRREDGYHLLQTVFRFLDFADE
                     LEFVSRRDGQIRLHTPLPEVPEEKDLCVRAARLLQQESGTGLGVDIFLKKRIP"
     gene            complement(296..937)
                     /gene="lolB"
                     /locus_tag="VFI43_07470"
     CDS             complement(296..937)
                     /gene="lolB"
                     /locus_tag="VFI43_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HET7062001.1"
                     /translation="MCAPPLLSGCSALAPSHKDTVVKTILIEPAADAAHLGSPNFLFA
                     GRVSVTGGKESFSSGVRWRHSEAGDEIILLSPLGQTLAQIQLGQDGAYLTTSEQQGYY
                     APDVESLTEQVLGWHLPVAGLQYWVQGISSPVTPAEVDVDESGRIVAIRQDGWTIDYL
                     GYFPARASSAVPLDMEELPPPPTAQPRLLRLKRDALQIKLVIDAWNPIAADGA"
     gene            complement(1014..2744)
                     /locus_tag="VFI43_07475"
     CDS             complement(1014..2744)
                     /locus_tag="VFI43_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7062002.1"
                     /translation="MILKLPGTLLLLTFCAACAQIPGKPDTKASANKSAAKGEAGHSK
                     LPNQDLTAPMLFDFLLAETALQRGDQELAIQTYLKLARTTRDPRVAQRATEIALHARQ
                     AAQALEAAKLWVEFEPDSVTARQTIAALLVNVDRLEEARPHLQKLLAAEGANLSEAFM
                     QLNSLLARNPNKNATFELVKQLTQPYPDLPEAHFAVSQAAWFANQFDTALAEMKKALE
                     LRPDWEVAAIYQGRILARNSNASAIEFFEKYLKSHPKANDTRITYARLLLAEKDYAKA
                     RQQFQQLLAENPGNADVAVAVGLLSLELRDYDAAESNFKKALQLDYRDPGMVRFYLGG
                     VYEKTQQFHAAMESYLSVTSGSPQYLPAQIRYALLLSKNGKMNEALQHLEHLPVTTDQ
                     QRAQLIVAEAQLLREAGSYQKAFRLLSDGLEKFPDSMELLYDRALAAEKIGKTDLMEQ
                     DLRKLIKLKPDHAHAYNALGYGLAEHTNRLKEALELIEKAIQLSPNDPYIMDSLGWVH
                     YRMGNLNQGLSFLKQAFGMNHDPEIAAHLGEVLWVQGTRDEAKEIWQSALKNHPGNEV
                     LVSTMKKFMK"
     gene            2998..3864
                     /gene="mutM"
                     /locus_tag="VFI43_07480"
     CDS             2998..3864
                     /gene="mutM"
                     /locus_tag="VFI43_07480"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET7062003.1"
                     /translation="MPELPEVEVIRRGITPHIEGRCIVGTMVRNAALRWTVPADLAQI
                     LDGFFIAKVSRRAKYLLLGSDKGTLILHLGMSGRLRLIPASAAEPPGKHDHVDLLLDT
                     GAVLRFRDPRRFGAILWEAGDVTRHPLLAKLGPEPLTEMFTADLLYKATRGRSVSIKQ
                     LLMNSSVVAGVGNIYANEALFRAGINPLTAAGDIGMGGYRKLVQAIKDTLDLAIEAGG
                     SSLRDFVNSEGNPGYFQQQYWVYGRAGRPCRKCGSAIRQIRQGQRSSFYCPGCQKAAS
                     TKEGADHVEDSA"
     gene            4010..4819
                     /gene="proC"
                     /locus_tag="VFI43_07485"
     CDS             4010..4819
                     /gene="proC"
                     /locus_tag="VFI43_07485"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7062004.1"
                     /translation="MTISFIGGGNMTRALIGGLLKQNSPAGMRVVEIDAEQRDRIRLE
                     FNLEVYAELDKGVAGSDTVVLAVKPQQLSAVARELAPLLSGHLVISIAAGIRAADISR
                     WLGDYSKIVRAMPNTPALAGSGITGLHALPAVDEKGKLDAETLFAGVGSVLWCPHEEM
                     LDVVTAVAGSGPAYVFYFMEAMQKAAVELGQTAAQARQLTIETFLGAVRLTIQSNEDA
                     SILRARVTSPGGTTERAIRSFEADGVQNAIVKAIHLAHERSQELGDEFGKT"
     gene            4852..5424
                     /locus_tag="VFI43_07490"
     CDS             4852..5424
                     /locus_tag="VFI43_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7062005.1"
                     /translation="MLNQMLVFLLDTLLGLFSLALLLRFYMQLVRAPYGNPVSQFLMS
                     ITDFVVRPARRIIPSLRGIDLPTLLLAWLAQFALLASVYMLQGYNFSSTMGIAAAGLS
                     LLALLEIIKMTLYIVMAAVIIQAVLSWVNPHSPLAPLLDSFTRPFLGVFRKRIPPIGN
                     VDLSPFFILIVIQLLLFLVAGVEKGISRLF"
     gene            5426..5734
                     /locus_tag="VFI43_07495"
     CDS             5426..5734
                     /locus_tag="VFI43_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET7062006.1"
                     /translation="MISQAWWRYETNDKGRRLILNLHIQPGAKRTEVAGLHGDALKIR
                     LAAQPLEDAANSALLEFLCRIFDVPLRQVILKRGTHSRRKIVEIREPAREAKALYETG
                     "
     gene            complement(5796..>6865)
                     /locus_tag="VFI43_07500"
     CDS             complement(5796..>6865)
                     /locus_tag="VFI43_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062007.1"
                     /translation="SKQLLMNIARAHHHQPVHFTGVSNVAATFDFRFSAGGTPALTGN
                     AGGMLLPVFGGSVAENPTISIVPIEGEEFTRRLLTPFQQNKLTLLLRQRYDIDLLLRL
                     MAQEVRIQNAKQETGYRNRPSDPSGYEMFRRIVLHLSAIQDQNQLYAEPLNLEQTWTI
                     PGASVSGDSFLALQKEFSVLYNRQNDVYTLRKQSVGPILITNYDPDTLSPHERARLNE
                     EAEDWIGNDVAFDIRPGHPGGEWPMRGVFRLRSFHAILSFLGRSVGDEPEYHVDPDPR
                     TPALLNNENPVFAMEVVAKDDPPPKEETSVYSHGKYYFVNIDGPLSRWDRDAFQMLYL
                     LFQMTITDMPRVGVPSITIAK"
BASE COUNT         1523 a         1855 c         1906 g         1581 t
ORIGIN      
        1 atcggaatac gcttcttcag gaaaatatcc acgccgagcc cggtgccgct ctcctgttgc
       61 aacaacctcg ctgcgcgcac gcataagtcc ttttcctccg gcacttcggg caggggcgta
      121 tgcagtctta tctgcccatc ccgccgcgaa acgaactcca gttcgtcggc aaaatcaagg
      181 aagcgaaaaa ccgtctgcaa gagatgatag ccatcttctc ttcggccaac aatgtggagg
      241 aacaggttaa gttttgctgg agcagggcaa ataaggcagt tatcctcgct cacagctacg
      301 ctccatcggc ggcaattgga ttccacgcat cgatgacgag cttgatctgc agcgcgtcac
      361 gcttgagccg caagagtctt ggctgggcgg taggtggcgg cggcagttct tccatatcca
      421 ggggaacagc ggatgatgca cgtgccggaa aataaccgag ataatcaatc gtccatccat
      481 cctggcgaat cgccacgatc ctcccgctct catcaacatc cacttcagct ggcgtgacag
      541 gcgaactgat cccttgaacc cagtattgca aacccgcgac cggcaagtgc caccccagca
      601 cctgttcagt caggctttcg acatcaggag cataatagcc ctgctgttcc gaggtggtta
      661 agtatgcccc gtcctgccca agctgaatct gagcgagtgt ttgcccgagt ggcgaaagca
      721 gaattatttc gtcccctgcc tcggaatgac gccaccgaac tccgctcgaa aagctttcct
      781 ttccgcccgt cactgaaacc cttcccgcga acagaaagtt tggcgacccc aggtgcgcag
      841 catccgcggc cggctcgatc agaatcgttt ttacgacggt atctttatga gatggagcca
      901 gtgcggagca accggacaat aaaggtggtg cgcacaagaa cagactccac cagggaaatg
      961 gaaaacgcgg cggctggcgc gattttaggc cggcatttac ttccatcctg gtcctatttc
     1021 atgaatttct tcattgtact gaccagtacc tcattgccgg gatggttctt gagtgccgat
     1081 tgccagattt ccttggcctc gtccctcgtt ccctgcaccc aaagcacctc acccaggtga
     1141 gccgcaattt cgggatcgtg gttcattccg aaagcctgtt tcaaaaagct gagcccctgg
     1201 ttgagattgc ccatccggta atggacccag ccaaggctat ccatgatata ggggtcattg
     1261 ggagaaagct ggatcgcctt ctcgatcagt tccagggctt ccttgagacg gttggtgtgt
     1321 tccgcaaggc cgtagccaag ggcgttatac gcgtgcgcat ggtcgggctt gagcttgatc
     1381 agcttgcgta gatcctgctc catcaagtcg gttttgccga tcttttcggc tgcaagcgca
     1441 cggtcgtaca aaagctccat cgaatcagga aatttttcaa gcccgtcgct gagcaatcgg
     1501 aatgctttct ggtagcttcc cgcttctctc aacagctgag cctccgcgac gataagctgc
     1561 gcacgctgct gatcggtggt gacggggaga tgctccaggt gctgaagcgc ctcgttcatt
     1621 ttcccgtttt tgctcaacag cagcgcgtac cggatttgag cgggaagata ctgcgggctt
     1681 ccgctcgtga ctgacaggta cgattccatg gccgcgtgaa actgctgcgt tttttcgtaa
     1741 acacccccga gataaaagcg gaccatcccc gggtccctgt aatcgagctg caatgccttt
     1801 ttgaaatttg actccgctgc atcgtaatcg cgaagctcta gcgacagcaa accgaccgct
     1861 acggcgacat cggcgtttcc cggattctcg gcaagcaact gctggaactg ctgccgcgcc
     1921 ttcgcgtaat ccttttcggc gagaagcagg cgggcgtaag taatccgggt gtcattcgct
     1981 ttcggatggg atttgagata tttttcaaag aattcgatag cactggcatt ggaattacgc
     2041 gcaagtatgc gtccttgata gattgcggca acctcccagt ccggcctgag ttcaagggcc
     2101 tttttcatct ctgccagagc cgtatcgaat tgattcgcaa accacgccgc ctgagatact
     2161 gcaaagtgag cttcaggaag atcaggatag ggttgagtaa gctgcttgac cagctcgaat
     2221 gtcgcattct tgttcgggtt gcgcgccagg aggctgttca attgcatgaa cgcttcgctg
     2281 agattggcgc cttcggctgc cagcagcttc tgcaaatgag gccgagcctc ctccagtcgg
     2341 tcaacattga ccagcaacgc ggcgatagtc tggcgcgcgg ttactgagtc aggctcaaac
     2401 tcaacccata gctttgcggc ttccaacgcc tgcgccgctt ggcgcgcgtg aagggcaatt
     2461 tcggtagcac gctgggcaac tcgcggatca cgcgtggttc tagccagttt caggtatgtt
     2521 tgaattgcaa gttcctggtc tccacgctga agggcggttt cagcaagcag aaaatcgaac
     2581 agcatgggtg ctgtcagatc ctggttgggc aatttggaat gcccggcttc acccttcgct
     2641 gctgatttat tcgcgctcgc cttggtatcg ggcttgcccg gtatctgcgc gcaagcggca
     2701 caaaaagtca gcagcaacaa tgtgccagga agtttaagga tcatgtgggt aaatccgggc
     2761 aagtttgaaa ataggacagg gcgaagcact gccttccggc acaaatgcca catattaggt
     2821 atattttacc cttatcacaa gtgcgcagcc gtgtggcaag atgcggcgga cgtccatccg
     2881 tcccttggaa cagcaaacaa atgcaacgcg taaccaatat gttagggaga cagggatgct
     2941 ttcatggatt ctcggtggga tcactatccc ctctgactca gtcccttaag caccatcatg
     3001 cctgaactac ccgaagtaga agtcattcgc cgaggcatta ccccccatat cgagggccgg
     3061 tgcattgtgg ggaccatggt aagaaacgcg gcgctgcgct ggaccgtgcc cgccgatctg
     3121 gcgcagatac ttgacggttt tttcatcgcg aaagtttccc ggcgggcaaa atacctgttg
     3181 ctcggctctg acaagggcac tctcatcctg catctcggca tgtccggaag actcaggttg
     3241 atccctgcgt ctgctgccga acctccgggc aagcatgatc acgtcgatct gttgctggat
     3301 accggcgctg ttcttcgctt cagggacccc cgccgtttcg gcgcgattct atgggaagcc
     3361 ggggacgtca cgcggcaccc cctgttagcc aagctgggtc ccgaacccct caccgaaatg
     3421 ttcaccgccg accttttgta caaggcaacg cgagggcgga gcgtcagcat caagcaattg
     3481 ctgatgaata gtagcgtggt tgcgggcgtg gggaatattt acgcaaacga ggctcttttt
     3541 cgggcaggca tcaatccgtt gactgcagca ggggacatcg ggatgggcgg atatcgaaaa
     3601 ctggtgcaag cgataaagga tacgcttgat cttgcaattg aagctggagg cagcagcctg
     3661 cgggatttcg tcaacagcga gggcaatcca gggtatttcc agcagcagta ctgggtatat
     3721 gggcgagcag ggcgcccctg ccggaagtgt ggatcggcga taagacaaat ccggcaaggc
     3781 cagcgctcca gcttttattg cccgggctgt cagaaagccg catcgacaaa agaaggcgcc
     3841 gatcacgttg aagattctgc gtgaacttgc cgcttcctac tccacctctt tgaagttcgc
     3901 agcagaaaat aagagcataa taccttaatt cacttttctc cctttctgca cgcggcgtca
     3961 gtttcccgcc acttcttctc acggtcttga atatttccag gattaaaaaa tgacgatcag
     4021 tttcattggc ggcggcaata tgacacgggc tctgatagga gggctgctga agcagaattc
     4081 accagcgggg atgagggtgg tggaaattga cgccgagcag cgcgacagga tacggctgga
     4141 attcaatctg gaggtatacg ccgagctcga caagggggtt gccggcagcg ataccgtcgt
     4201 gcttgcagtc aagcctcagc aactctccgc tgtcgctcgc gaactggcgc cactgctttc
     4261 aggccatctc gtcatttcca ttgctgcagg cattcgggct gcagatattt cccgctggct
     4321 gggggactac tcgaaaattg tacgcgcgat gcccaatact ccagccctgg ctggttcggg
     4381 tattaccggc ctgcatgcgc ttccggcggt tgatgaaaaa ggaaaacttg acgcagagac
     4441 cctgtttgca ggggtgggat cggtgctgtg gtgcccgcac gaagagatgc tggacgttgt
     4501 gacggctgtg gccggcagtg ggccggcgta tgtattttat ttcatggagg cgatgcagaa
     4561 agcagcggtt gaacttgggc aaactgcggc ccaggcacgg cagctgacaa ttgaaacgtt
     4621 cctgggcgct gtaaggctta ccatccagag taacgaagac gcgagcatac tgcgtgcacg
     4681 ggtaacatcg ccggggggaa ccacagaacg cgcgatccgc tcctttgaag ctgacggggt
     4741 gcagaatgcg atagtcaagg caatccacct ggcgcacgag cgatcccagg agttgggcga
     4801 tgaattcggc aagacataac gttaccgtaa atatcggttg cagggctcgc catgcttaac
     4861 cagatgcttg tatttctgct ggacacttta cttgggctat tttccctggc gttattgctt
     4921 cgtttttaca tgcaactggt gcgcgcccct tacggcaacc cagtgtcgca atttctcatg
     4981 agtatcactg acttcgttgt acggcccgcc cggcgaatca tcccgagctt aaggggtatc
     5041 gatcttccga cactgctact ggcgtggctt gcacagttcg ccctgctggc aagcgtatat
     5101 atgcttcaag gctacaattt cagctcgacc atgggcattg cggccgctgg attgagcctg
     5161 cttgcgctcc tcgaaattat caaaatgacg ctttatatcg taatggcagc ggtgatcatt
     5221 caggcagtac tgtcctgggt caacccccac agcccgcttg cccctctgct cgacagtttt
     5281 acccgcccct ttctgggagt gttccggaag cggatcccac ccatcggaaa tgtggaccta
     5341 tccccgtttt ttatcttgat cgttattcag ttgctgttat ttctggtggc gggagtggaa
     5401 aagggaattt cccgtttatt ctgatgtgat aagccaggcg tggtggcgct acgaaaccaa
     5461 tgacaaagga cggcgcctta tccttaatct gcatatccag ccaggggcga aacgtacgga
     5521 ggtagcgggc ttgcatgggg acgccttgaa aataaggctg gccgcacagc cgttggagga
     5581 tgccgccaat tcagcgctgc ttgaatttct gtgcagaatc tttgatgttc cgctccggca
     5641 ggtaatcctg aaacgcggta ctcactcgag gcgcaagatc gtcgagatac gggaacccgc
     5701 gcgggaagcc aaggcgttgt acgagacagg ttgacgataa gtaaaagggc gggcagtttt
     5761 caagttcaga ttcttatcga actcactcga cagtctcact tggcaatagt aatgctcggt
     5821 acgccgacac gaggcatgtc ggtaatagtc atctggaata atagatacag catctgaaac
     5881 gcgtcccgat cccaccggga gagcggaccg tctatgttca cgaagtaata tttgccgtgt
     5941 gaataaacag atgtttcttc ttttggagga ggatcgtctt tagcgacaac ttccatggcg
     6001 aaaaccgggt tctcattgtt caagagagcg ggagttcgtg gatcgggatc cacgtgatac
     6061 tccggctcgt cgccgacgga gcgaccgagg aaactgagaa tcgcatggaa actcctcagc
     6121 cggaaaaccc ctctcatcgg ccactccccg cccggatgac cgggacggat atcgaatgcg
     6181 acgtcattgc ctatccagtc ctccgcctcc tcgttcaacc tcgcgcgctc gtgcggggat
     6241 aacgtgtcgg ggtcgtaatt cgtaataagg atcggcccta ccgattgctt gcgaagcgta
     6301 taaacgtcat tctgtctgtt atagagaacg gagaattcct tttgtagagc cagaaaactg
     6361 tccccggaca cagaagcgcc cgggatggtc cacgtctgtt caaggttcaa aggttccgcg
     6421 taaagctggt tctggtcctg aatggccgac agatgcagaa cgatgcgccg gaacatttcg
     6481 taccccgatg gatcggatgg ccggttacga tagccggtct cctgctttgc attctgaatg
     6541 cgaacttcct gcgccatcaa tcttagcaag agatcaatat cgtagcgctg gcgaagcaaa
     6601 agggtaagct tgttttgctg gaacggggta aggagccgtc gcgtaaattc ctctccttcg
     6661 ataggcacaa tgctgatggt gggattttca gccacgctgc ctccgaatac gggcaaaagc
     6721 atgccgccag cgttgcccgt cagcgcaggc gttccccccg cgctaaagcg gaaatcgaag
     6781 gttgctgcga cgttggaaac tcccgtaaag tgcacgggct gatgatgatg ggcgcgagca
     6841 atattcatca gcagctgttt ggatc
//
LOCUS       DASSHJ010000193         9631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1207526,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000193 DASSHJ010000000
VERSION     DASSHJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207526"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFI43_07505"
     CDS             complement(<1..848)
                     /locus_tag="VFI43_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7062008.1"
                     /translation="MDETRRNFMKGAVTGGALLALGIPPINPASAAEPLSNTTRSFRL
                     LLGNTQFDKIFADGARVAYSAYSRYASLTGSPPPQLSTVTLKGGLLAEPGRMAELLNQ
                     SRDVRWIAIMDDASAAVFVELARGANACLLSLGLHSASAEAAFSSATSPFLRHAWTAA
                     SSSHGPGELLASLLVHNQNHFTIMEHFLGQPSQNQTAAGGSMQGFVSYRGQGEAQGET
                     HLHCAGISPQDGCDLVGWKSPGGWGPLPTAADQNQTAKNGTAADQGRRAVNWRPENWV
                     EATGYTV"
     gene            complement(882..1223)
                     /locus_tag="VFI43_07510"
     CDS             complement(882..1223)
                     /locus_tag="VFI43_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7062009.1"
                     /translation="MKKAKPGASIACFLLAVIAAGGIAAQNVSAAEQEPPGQGFAWKD
                     GAQVYAGICVYCHESGVGPPIRNRDLPSAYIRTVVRNGNRAMPAFRPSEIDDESLAKL
                     ADFISGSKARP"
     gene            complement(1366..2001)
                     /locus_tag="VFI43_07515"
     CDS             complement(1366..2001)
                     /locus_tag="VFI43_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HET7062010.1"
                     /translation="MTAGQRYFMFEMGMICAFLGACSILPQAETIELYQLPSSSITNT
                     AHADKLPGTVRVSTPFGSRVTDSQQVLIVNEDNRINAYKGARWSDSASVLVRNRLTNA
                     FRADGRLNTIGHDNADLLPDLELDGELAAFQVEYVEGKPAVIIRMYATLVQPARYKAI
                     ATNGFEVMQRVEGTGMPEVIKAFGKASDRLAGEIVGWTVEQSKALQSKTQE"
     gene            complement(1998..2933)
                     /locus_tag="VFI43_07520"
     CDS             complement(1998..2933)
                     /locus_tag="VFI43_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7062011.1"
                     /translation="MEPRAHHVLIGLFTVIAVTAALLFALWLNKSSADEQGYYVVVFN
                     ETVRGLSKGSEVQYNGIKIGEVVELELDPQDPRRVLARIRVNGKAPIKQDTKARLALT
                     SITGNSVIQFSGGTPDSPDLVSPDGEEPVIVATPSPFAKLIEESGDNLAELTELIQRA
                     GEILSPENVQHFSKILINLDRASGTLASQDENFRELIQQLTASSRQANSVLQQASKLV
                     GNAQSLVDDEGARALGSAGRAMASLENSANALDQLLRDNRAGLAGGVRGLNELGPALQ
                     ELRETLISVRRIASRLEDSPASYLTGREKIEEVEP"
     gene            complement(2933..3781)
                     /locus_tag="VFI43_07525"
     CDS             complement(2933..3781)
                     /locus_tag="VFI43_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7062012.1"
                     /translation="MVTDEAAGRRQEPDAQAVIRIRNLTNRFGDQVVHDNLNLNVYRG
                     EILGVIGGSGSGKSVLLRSIVGLRTPASGTICVFDEDLLKLPRKERVRLERRFGVLFQ
                     GGALFSSLTVTENIAVPLIENGGLERADAERLAEVKLALAGLPAEAGEKYPSALSGGM
                     IKRAALARALALDPDILFLDEPTAGLDPIGATEFDQLTLTLRDALGLTVFIVTHDLDT
                     LYAICDRVAVLSHKRILVVGPLDKVVDTDDPWVQEYFNGPRGRAARNVRKAAPGRLAL
                     PVQEVD"
     gene            complement(3775..4923)
                     /locus_tag="VFI43_07530"
     CDS             complement(3775..4923)
                     /locus_tag="VFI43_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7062013.1"
                     /translation="MTDPALVTWDPSKNPPKLTVRGDWTLGNYAVLGEQIAHVRAEGA
                     DLTAAPRNVDLTGLKAIDTSGASRLYELLGENLARKVSSGNSTLSTGSIQLLQTVAAA
                     MKNTGEIPHKPPQNAFMEFFARVGLGMERFWREIVAMLSFSGLTLEALARGLLRPRSW
                     RITSVVAQIQATGMDAVPIIVLLNFMVGAVIAFLGATVLADFGAGIYTVNLVTFAFLR
                     ELAVLLTAILMAGRTASTFAAELGSMKINEEIDATRVLGLSPINLFILPRILALFVSL
                     PILTFLAMMAGIAGGALVCALELNISPAMFLNIFHEQIPLRHFLLGMSKAPVFAFIIA
                     VIGCLEGLRVGGSALSLGQHTTASVVHSIFVVIFLDAMAALFFMEMGW"
     gene            5405..6295
                     /gene="dapA"
                     /locus_tag="VFI43_07535"
     CDS             5405..6295
                     /gene="dapA"
                     /locus_tag="VFI43_07535"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7062014.1"
                     /translation="MQSALRGSLVAIVTPMHEDGELDLERFHGLIEFHIAQGTDGIVV
                     AGTTGESPTVDFDEHHLLIRSAVRYAAGRVPVIAGTGANSTREAIELAIYAKDAGADA
                     SLSVVPYYNKPTQEGLYRHFRAIAEAVDIPQILYNVPGRTVADISNETVLRLAEIPNI
                     VGIKDATGDIGRGFDLLCGIPENFSVYSGDDASALALLLLGGHGVISVTANVAPKVMH
                     EMCLAVFAGDLVAARAANKKLLRLHIDLFVEANPIPVKWAVAQMGLIGPGLRLPLTPL
                     SDRYHALVRDAMRWAGITVN"
     gene            6315..7439
                     /gene="bamC"
                     /locus_tag="VFI43_07540"
     CDS             6315..7439
                     /gene="bamC"
                     /locus_tag="VFI43_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HET7062015.1"
                     /translation="MKWRVWIALCMVVNLAGCQLMSLLPEAKKINYKSAGKLPPLEVP
                     PDLTAPGSDERYVVPDISPTGSATFSAYSKDRTKLAGVDGAAVLPAADETGVRMERSG
                     TQRWVVVKGEPEDIWPVVKNFWQELGFIIKLELPEAGVMETDWAENRAKIPDDLIRSA
                     LSKILDTLYSTAERDKFRTRLERGETGTTEIYISHRGMDEVMEGNVRTVWQPRPPDPD
                     LEAEMLSRLMIRFGATEERARSSMASAGTARERARLGEKGRALTVNEAYDRAWRRVGL
                     ALDRIGFTVEDRNRSQGIYYVRYVNPDEDSGAGTGAKGLLSKLIFWSQGEDTDQPGQY
                     RIQLSEAGQATDVKVLNKEGGAEKSSDATKILKLLYDQLK"
     gene            complement(7504..8397)
                     /locus_tag="VFI43_07545"
     CDS             complement(7504..8397)
                     /locus_tag="VFI43_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062016.1"
                     /translation="MDQVKYRSVFSPASAQQRQENFEAYWQFTQRHSGALIEHDKDLS
                     EKRIRLRQFQSNPVKLRCPLTDPEGFYRNYIKMEDDPASLDRMTLMLTCLYKFARHEW
                     VGIKGAWEAVPDMAHSFNIVDKITRVHLAEEFCHVRLFDEMLRTCGLDKVHWVPLSPF
                     QEKIYEQFPKAPDFLIAAPAFVTELMGVTFYCHLARLIDELLAEEPEVRGRLHQLLDE
                     ITIDETAHVGLRRNFLGPLGVRISRALVKPFYIHFFEDIPEIKHLFDVNQMVKDGIAF
                     DFNQVPRYMIEHSWIPSYCRE"
     gene            complement(8475..9512)
                     /locus_tag="VFI43_07550"
     CDS             complement(8475..9512)
                     /locus_tag="VFI43_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HET7062017.1"
                     /translation="MPASLFPSIGLTAPFILAGMAIVAFSGSVSARENSSRELVVTNR
                     YTLPLLEISGLAPAPALDSKGAQTANRSLRLYAVGDATREVVTFYIDGSSGGIVISLK
                     DAALPSGRRAAGASQWEAVAADGVDTVCMLSETDSKVSCFDDRINENRGIITLDTSPL
                     KHLNRLWGNRPNSRGEGMLLMKKGHLLILKEKKPSLLVEFGPQGDLATGYGPDTFLAP
                     DEAFALPSRAPGQQEPTLVALKSWEFSDRLRELASDASEITLGPDGHVYLLSHESSTL
                     IRLEKVLKPDEEKVNINDDAWWILPKGIGKTEGLAIDSEMRPWIAVDRKQKEKPNLFR
                     LSLISPGAEGD"
BASE COUNT         2103 a         2632 c         2754 g         2142 t
ORIGIN      
        1 accgtataac cggtcgcttc aacccagttt tcgggcctcc agttgactgc gcggcgcccc
       61 tggtctgcgg cagttccatt tttggctgtt tggttttgat ctgctgccgt gggaagcgga
      121 ccccatccac ccggtgattt ccagccaacc agatcgcatc cgtcctgagg ggaaatgccc
      181 gcgcaatgca gatgggtttc tccttgggct tccccctgcc cgcggtagga tacaaatccc
      241 tgcatagatc cacctgctgc tgtttggttt tgcgaaggct gcccaagaaa atgttccata
      301 atcgtgaaat ggttttgatt gtggacaagc agcgaggcca gcagctctcc agggccatgc
      361 gaggatgacg ccgctgtcca ggcgtggcgc aggaaaggcg aggtggcgga agagaaggct
      421 gcttcggccg aagcactatg gaggcccagc gacagcagac aggcgtttgc gccacgcgcg
      481 agttcaacaa atacggctgc gctggcatcg tccatgatgg caatccacct gacgtcccgc
      541 gactgattca gtaattccgc catcctgccg ggctcagcca gcaagccgcc ttttaatgtc
      601 accgtgctca actggggagg gggcgaaccg gtcagcgatg cgtaccggga gtaagctgag
      661 taagcgacgc gtgccccgtc ggcgaagatc ttatcaaact gcgtattgcc gagcagcagg
      721 cggaagctcc tggtcgtatt ggacaggggt tcggccgccg acgcagggtt gatgggggga
      781 atgccaaggg ccagcagcgc acctccggtc acagcgcctt tcatgaaatt acgtctggtc
      841 tcgtccatcg aaggcagtcc tttcaagggc cttgcttcgg tctagggcct tgctttgctt
      901 ccggaaatga aatccgcgag cttcgccagc gactcgtcat cgatttccga aggccggaag
      961 gcgggcatcg ccctgtttcc attccgaacg acagtacgga tataggcgga aggaaggtcg
     1021 cggttgcgaa tgggcgggcc aacgcctgat tcatggcagt atacgcagat cccagcgtat
     1081 acctgcgcgc catctttcca cgcaaaccct tgccccggcg gttcctgctc tgccgccgat
     1141 acattttgtg ccgcgattcc ccccgcggct atcaccgcaa gcaaaaaaca ggcgattgat
     1201 gctcccggtt ttgccttttt catgttcagt ttccgaagaa cgcttgtttg aagcgagcac
     1261 ttttaacgca cgcctcggca acgcctcatt tcatcttagg cgaaaggcgg cacagtgttc
     1321 ccgttttatc caggatgatg gccaacgttc ttcatatccg tttgtttatt cttgcgtctt
     1381 ggattgcagc gccttacttt gttctactgt ccagccgaca atctctcctg cgagcctatc
     1441 ggaagctttt ccgaaagcct tgatcacttc aggcattccc gtgccttcga cccgctgcat
     1501 gacttcgaat ccgtttgtgg ctatcgcctt atatcgcgcc ggttgcacga gcgtggcgta
     1561 catgcggatt atcacggctg gcttcccttc gacgtattcc acctggaagg ctgcgagttc
     1621 tccatccagt tcgagatcgg gaagcaggtc ggcattatcg tgacctattg tgttcagccg
     1681 cccgtcggcc cgaaaagcat tcgtgagccg gttgcgaacc agtacggaag ctgaatcgct
     1741 ccagcgcgcg cccttgtagg cgttgatccg gttatcctcg tttacgataa gcacctgttg
     1801 gctatccgtg actcggctcc cgaagggggt ggaaacccgc acggtcccgg gaagtttatc
     1861 tgcgtgcgcc gtgttcgtaa tggaagagga aggcagttga tatagctcga ttgtctctgc
     1921 ctggggaagt atcgagcagg cgcccaaaaa cgcgcatatc atccccattt cgaacatgaa
     1981 ataccgttga cccgcggtca tggttcgacc tcctctattt tttcgcggcc cgtgaggtaa
     2041 ctggcaggac tatcctcaag ccggctggct attcttctta ccgagatcag tgtctccctg
     2101 agttcctgca aggcagggcc aagctcgttg aggccacgaa cgccccccgc aaggccggcg
     2161 cgattatcgc gcaggagttg atcgagggca ttcgcgctgt tctcgagcga agccatcgcc
     2221 cgtccggcgc tgccaagcgc gcgtgcgccc tcatcgtcga cgagtgattg cgcgttgccc
     2281 accagcttcg atgcttgctg caacaccgaa tttgcctgcc tgctggatgc cgtcaactgc
     2341 tgtatcaatt ccctgaaatt ttcgtcctgg cttgcaagag tgcccgaagc gcgatccaga
     2401 tttatgagaa tcttgctgaa gtgctgaaca ttctcaggcg agagtatttc cccggcccgc
     2461 tggatcagtt ccgtaagctc agccaagttg tcgccgcttt cctcaatcaa cttcgcgaag
     2521 ggtgagggcg tggcgacgat gacgggctct tcgccatctg gggagacaag atctgggcta
     2581 tccggtgtcc cgccgctgaa ctggatgacg gagtttccgg taatgctcgt caaggccaac
     2641 cgggccttgg tatcctgctt gatcggggcc ttgccgttca cgcgaatacg ggcgagaacc
     2701 cggcggggat cttgcggatc gagctcaagc tccaccactt ccccgatctt tataccgttg
     2761 tattgcactt cgcttccctt cgacaggcct cttaccgttt cattgaaaac cacaacgtaa
     2821 tagccctgtt cgtcagccga cgacttgttc agccacaagg cgaacagcaa ggcggcggtg
     2881 acggctatta ccgtaaacag tccgattaga acatgatgcg cgcgaggctc catcaatcga
     2941 cctcctgcac gggcagcgcg agtcgtccgg gagcggcttt ccttacgttt cttgctgcgc
     3001 gtccgcgcgg tccgttgaag tactcctgca cccagggatc atcggtgtct acgactttat
     3061 cgagcgggcc gaccaccagt atccgcttgt gggaaagcac ggcgacgcga tcgcatatgg
     3121 cataaagcgt atccagatca tgcgtgacga taaaaacggt gaggccgagg gcatcgcgca
     3181 gcgtgagggt aagctggtca aattcagtgg cgccgatagg atcgaggccc gccgtcggct
     3241 catccaggaa caggatgtcc ggatcgaggg cgagcgctcg cgccagtgcc gcacgtttga
     3301 tcattccgcc cgagagggcg gatggatatt tctctccagc ttcagcggga aggccggcaa
     3361 gcgcaagctt gacttcggca agacgttctg cgtctgcccg ttccaggccc ccattttcaa
     3421 taagcgggac tgcgatattt tcggtgacgg tcagcgacga aaacagtgcg cccccttgga
     3481 agagcacgcc aaagcggcgc tcgagccgga ctcgctcctt gcggggcagc ttcagcagat
     3541 cttcgtcaaa aacgcatatc gtgccggagg cgggtgtccg caatccgacg atcgaccgca
     3601 gaagaacgga tttgccggag cccgagccac cgatcacccc caggatctcg ccgcggtaga
     3661 cattcaggtt caaattgtcg tgcacgacct ggtcgccgaa tcgattcgtc agattgcgaa
     3721 tccggatcac cgcttgggca tccggttcct ggcgcctccc ggccgcctca tccgtcacca
     3781 tcccatttcc atgaaaaaca gcgcagccat cgcatcaaga aaaatcacca cgaaaattga
     3841 atgcacgaca ctggcagtcg tatgttggcc gagcgacaac gcgctgccgc cgactctaag
     3901 accttcgagg cagccgataa cggcgatgat gaaggcaaaa acgggtgcct tggacattcc
     3961 cagcaggaaa tgccttaaag ggatttgctc atggaaaata ttgaggaaca ttgcgggcga
     4021 gatattcagt tccagcgcgc acacgagcgc gccacccgct attcctgcca tcatggccag
     4081 aaacgtgaga ataggcagag agacgaacaa tgccagtatg cgtggcaata tgaacaggtt
     4141 gatagggctt agacccagta cccgggttgc gtcgatctct tcgttgatct tcatcgaccc
     4201 cagctctgca gcgaacgtgc ttgcggttcg ccctgccatc aggatagcgg tcagcaggac
     4261 agcgagttcg cgcaaaaacg caaaagtcac caggttgacc gtatagatgc cggcgccgaa
     4321 atctgcgagg acggtggccc ccaggaaagc aattaccgcg cccaccatga aattcaaaag
     4381 cacaatgatg ggcacggcgt ccatgccggt cgcctggatt tgcgcgacca ccgaagtgat
     4441 gcgccagctt cgcgggcgca ggagacctcg cgccagcgct tcgagtgtca ggcctgagaa
     4501 actgagcatt gccactattt cacgccagaa tctttccatc cccaggccga cgcgggcaaa
     4561 aaattccata aacgcgttct gcggtggctt gtggggtatt tctccggtgt ttttcattgc
     4621 cgcagcgacg gtttgcagta actgaatgct ccccgtgctc aacgtcgaat tgccgctcga
     4681 aactttcctc gccagatttt caccaagcag ttcgtacagc cgcgatgccc cggacgtatc
     4741 gatcgccttg agcccggtca gatcaacgtt acgcggagca gcagtcaaat ctgctccctc
     4801 ggcgcgaacg tgcgcaatct gttcgcccag cacggcataa ttgccgaggg tccagtcgcc
     4861 acgcacagtg agctttggcg gattcttgga aggatcccag gtcaccaggg ctggatcagt
     4921 catgattttc gtgatgcata actccttgca cctaaaataa actcttactt agttatagca
     4981 gcccccggat gttgacactt gtgctagagg tgcgaccgag tgagaacggg gagcaaagca
     5041 gcttgaaggg aagaatctcg gaggccgcca tcggccaggg gatcattgca aggtatggca
     5101 ctcgcgcggg ttgggatgga aatgaaagtc cttaccggct cttgcgacac gaacgcagcg
     5161 ctcttttttt cattgaacct atcctgcctc tgcattggat tatcgctgtc gttgaaacca
     5221 ttgtccccga atcgtgtccg aagcaaggct gtcactcgcg gtgcaggtgc gatgatccgc
     5281 gatgaaagtt tcctggagcg agcggcggcg cattgccaag ttatggtcaa gtttgcgcag
     5341 cgattatttt tggcaacttc cagattaagg taaaataacc ggtttcaccg gatgggaata
     5401 ccgcatgcaa agtgcgttac gaggaagcct ggttgcaatc gtgaccccta tgcacgaaga
     5461 cggggaactg gatctggagc gttttcacgg cctgatcgag tttcacatcg cgcaggggac
     5521 ggatggcatc gttgtggcag gcacaaccgg agaatctcca actgtcgatt tcgatgagca
     5581 ccaccttctg attcgttctg cggtgcgata tgcggcggga cgcgtgccag tcatcgcggg
     5641 gacgggcgcc aattcgacgc gcgaggccat tgagttggcg atttacgcga aggatgcagg
     5701 ggcggatgca agcctgtcgg tcgttcctta ctacaacaag ccgacgcagg aagggttgta
     5761 tcgccacttt cgcgcaatcg ccgaggcagt cgatattcca cagattctct acaacgttcc
     5821 cggcaggact gtggccgata tttccaacga gacggtactg cggctggcgg agattccgaa
     5881 tatcgtcggg atcaaggatg ctacgggcga tattggacga ggtttcgatt tgttgtgtgg
     5941 gattccggag aatttttcgg tttatagcgg cgatgatgcg agcgcgctcg ctctgctctt
     6001 gctgggcggg catggcgtaa tttcagtgac ggccaatgtc gctccaaaag tgatgcacga
     6061 aatgtgtctt gcggtgtttg ccggggatct cgttgccgcc cgggcggcga ataaaaagct
     6121 gctgagactg catatcgact tgtttgtcga ggccaaccct atccctgtca agtgggcggt
     6181 ggctcaaatg ggattgatcg gccccggcct gcggttgccc ttgacacccc tgtcggatcg
     6241 atatcacgct ctggtcagag atgcgatgcg ctgggcagga ataacggtta attgatgaat
     6301 caagcgtata caacatgaaa tggcgggtat ggatcgcgct gtgtatggta gtcaatctgg
     6361 ctggctgcca actcatgagc ttgttgccag aagccaaaaa aatcaattac aagtcggcgg
     6421 ggaaactccc acccctggaa gtgccgcccg acttgacagc acctgggtcg gacgagcgtt
     6481 acgtcgtgcc ggacatcagt cccactggct ctgcgacatt ttccgcttac agcaaggatc
     6541 ggaccaagct tgcaggcgtt gatggcgccg cagttcttcc cgcagcggat gaaacgggcg
     6601 tgcggatgga gcgctccggg acccagcgct gggttgttgt aaagggcgag ccggaggata
     6661 tctggccggt agtcaagaat ttctggcagg agctgggctt tattatcaaa ttggaactgc
     6721 cggaagctgg ggtcatggag accgattggg cggaaaaccg tgcgaagata cccgacgacc
     6781 tgattcgcag tgcgttgtca aagattctcg atacgctgta ttcgaccgcc gagcgcgaca
     6841 agtttcgtac gcgtctggaa cggggcgaga ctggcacgac ggaaatctat atcagccatc
     6901 gtgggatgga tgaagtgatg gaaggcaatg ttcgcaccgt atggcagcca cgtccgcctg
     6961 atcctgacct tgaagccgag atgctttccc gcttgatgat acgatttggc gccacggagg
     7021 aacgggcgcg gtcgtcgatg gcgagcgcgg gaacggccag ggagcgcgcc cgcctgggtg
     7081 agaagggccg ggcactgacc gtgaatgaag catatgaccg agcgtggaga cgcgtgggac
     7141 tggcgctcga tcgtatcggc ttcacagttg aagatcgcaa tcgctcgcag ggaatctatt
     7201 acgtgcgcta tgtaaatcct gatgaggaca gcggcgccgg cacgggggcc aagggattgt
     7261 tgtccaagct catcttctgg agtcagggcg aggacaccga ccagccaggg caataccgca
     7321 tacagctcag tgaagcgggc caggcgacgg atgtcaaggt gctgaacaag gaggggggag
     7381 ccgagaaatc cagcgatgcg accaagatat tgaaactgct atatgaccag ttgaagtaaa
     7441 ctggtccgaa gcggaaggaa tatccattga gtcaaaagga gggctcgggg gcttctggca
     7501 gactcactcc ctgcaatagg atggtatcca gctgtgttcg atcatgtaac ggggaacctg
     7561 attaaagtcg aaagcaattc catccttcac catctggtta acatcgaaca gatgcttgat
     7621 ttccgggatg tcttcaaaga aatgaatata gaaaggcttg acgagcgctc ttgaaatcct
     7681 gacgcccaac ggacccagga agtttcttct tagcccgaca tgggcagtct catcaatggt
     7741 aatttcatcc agtaactggt ggagacgtcc cctgacttcg ggttcttccg caagcagttc
     7801 atcgatcagg cgtgccagat ggcaatagaa agtgactccc atgagctcgg ttacaaaagc
     7861 cggagcggcg atcagaaaat cgggggcttt gggaaactgt tcatagatct tttcctgaaa
     7921 ggggcttaaa ggcacccagt gaaccttgtc aaggccgcat gtgcgcaaca tctcgtcaaa
     7981 caggcggaca tgacaaaatt cctctgccag gtgcacgcgg gtgatcttgt ctacgatgtt
     8041 aaaggagtgg gccatatcgg gaactgcttc ccacgcgcct ttaattccta cccattcatg
     8101 tctggcaaac ttatataggc atgtcaacat tagcgtcata cgatccagcg aggcaggatc
     8161 atcttccatt tttatgtaat tgcgataaaa cccctccggg tccgtcaaag gacagcgcaa
     8221 tttgacagga ttgctctgga actgcctgag acgaatgcgt ttctcggata aatccttatc
     8281 atgttcaatc aatgctccgc tgtgccgctg ggtgaattgc cagtaggctt cgaagttttc
     8341 ctgcctctgc tgtgcggatg cgggggaaaa gacggaacga tactttactt ggtccatgga
     8401 tattcctcct gtcatcagct gcgggactcc ttataaatca tagcgaatat tatgtaatgg
     8461 agaacttcgc acgctcagtc accttcggct cctgggctaa ttagagacag tcggaacaga
     8521 ttaggttttt ccttctgctt tctgtcaacg gcgatccatg gccgcatttc tgaatcaatg
     8581 gcgagaccct cggtcttgcc aattcccttg ggtaggatcc accaggcgtc atcatttatg
     8641 ttaacctttt cttcatccgg cttcaggact ttttccaggc gaatcagcgt cgaactttcg
     8701 tgagacagta gatatacgtg accatccggg ccaagcgtga tttcgctcgc atcgctggcg
     8761 agttcgcgca ggcgatccga aaattcccag gatttgagcg cgacgagagt cggttcctgc
     8821 tgaccaggtg cccgggatgg caacgcaaag gcttcgtctg gcgccaggaa agtgtccggt
     8881 ccgtagcccg tggcgagatc cccttgcgga ccaaattcca ccagcaagga gggttttttt
     8941 tccttcagga tcagcaaatg gcccttcttc atgagcagca ttccttcacc acgcgaattt
     9001 ggccggttac cccaaaggcg gttgaggtgc ttcaagggtg aagtgtcgag agtaattatt
     9061 cctctgtttt cgttgatgcg atcatcgaag cacgaaactt tgctgtccgt ctcgctcaac
     9121 atgcaaaccg tatccacccc gtcggctgcg accgcttccc attgcgaggc gccggcggct
     9181 cgtcttcctg aaggtaaagc cgcatctttc aagctgatga caattccccc agatgatccg
     9241 tcaatataaa aggtgacgac ctcacgggtt gcatctccaa cggcataaag gcgcaagctc
     9301 cggttagccg tttgcgcccc tttactgtca agtgcaggag caggggccag ccccgatatc
     9361 tccagaagcg gaagcgtgta tcgatttgtg acaaccagtt cgcgcgagga attttcccgg
     9421 gcggaaacgg atccggagaa cgcaactata gccatgccgg ccaggatgaa aggggccgta
     9481 aggccgatcg acggaaataa tgatgcgggc atgccgggaa cctgaatcgt gcgccttgtg
     9541 atcatacccg attttatcag caatgcgctt cacgaggact tggttagaag tcctttaagt
     9601 ccgatgggca atgcgtgtga gggacgtaag a
//
LOCUS       DASSHJ010000194         8068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1208389,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000194 DASSHJ010000000
VERSION     DASSHJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208389"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFI43_07555"
     CDS             complement(<1..524)
                     /locus_tag="VFI43_07555"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7062018.1"
                     /translation="MNLALFDLDNTLLAGDSDFEWAQFLIEKRVLDREVYEARNLEFY
                     EQYKAGTLDIHEFLDFQLKPLSRHPRVQLDAWHSEFMKNRVLRLIAPRAHELIKQHML
                     GHDLCIIITATNSFVTGPIARALGIDNLVATEPEQKDGEFTGRVSGLPCFREGKIARL
                     ENWLDQRNLTWLSFL"
     gene            complement(521..1198)
                     /gene="hda"
                     /locus_tag="VFI43_07560"
     CDS             complement(521..1198)
                     /gene="hda"
                     /locus_tag="VFI43_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HET7062019.1"
                     /translation="MKQLLLGIAPPAQPTLANFVPGRNIELLQTLSSVVMGREGERLV
                     YLWGGPGSGKTHLLKAIAAICRRSKMKVAYFECQRDTRFVEGREADCIIVDGVDHLGA
                     DSQIGLFGLYNYIHDEGRAFMLASGPVPPAQLKMRADLVSRLGWGLVYQVHELTDEEK
                     IKAMKSHAASRGLDVPDEVCEYLLRHTQRDLSSLIAILDALDNYSLASQRKVTVPLIR
                     ELFEEES"
     gene            1307..2365
                     /gene="purM"
                     /locus_tag="VFI43_07565"
     CDS             1307..2365
                     /gene="purM"
                     /locus_tag="VFI43_07565"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041352649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET7062020.1"
                     /translation="MTSSKSENSASNPLSYRSAGVDIDAGDRLVENIKPFAKRTMRSE
                     VLAGIGGFAALFEISRNYRNPVLVSGTDGVGTKLKLAFVSDRHDSIGIDLVAMSVNDI
                     LVQGAEPLFFLDYFACGTLDVCTGTKVIKGIAEGCEQAGCALIGGETAEMPGMYPRGE
                     YDLAGFAVGIVEKNRIVTGSTIHEGDAVLGLASSGAHSNGYSLIRKIIEKNNIDLSAD
                     FGGQALIDVLMTPTRIYVKSILELMKHLPVKGMAHITGGGLIENIPRVLPDNLAAVLT
                     LNSWPVPPLFHWLQQQGNVADQEMFRVFNCGIGMVLIVAEEFAGDALMLLQSSGESAW
                     RIGRIEPHVANSPRAVIE"
     gene            2397..3035
                     /gene="purN"
                     /locus_tag="VFI43_07570"
     CDS             2397..3035
                     /gene="purN"
                     /locus_tag="VFI43_07570"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET7062021.1"
                     /translation="MKSVVILISGRGSNMQALMEARLPARISAVISNRPNAPGLAIAK
                     RHGIETIAVDQQSYPDRRAFDDALANTIEGYTPDVIALAGFMHILDNDLVSRYQGKLL
                     NVHPSLLPAFPGLFSHRRALQEGVKIHGCTVHFVTPEMDRGPIVIQAAIPVLPGDTEE
                     TLGARVLEQEHRIYPEAVRWFVEDRLKLSGNCVDISKAEPDGSVLYSPGLSS"
     gene            3032..3799
                     /locus_tag="VFI43_07575"
     CDS             3032..3799
                     /locus_tag="VFI43_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET7062022.1"
                     /translation="MKKFLAVLFFWLAFPAISALPASRGTVPSRVDISYSITAGNLEG
                     EVNDTVEIRQENGTRSYNIYSEGRAKGLLALTQPDNVVRDSEGTVTPKGVLRPARFSD
                     QHGKKLPKVAIFDWDKKSLTLQHKGKEEHRQLPADAQDKLSLLYSFMFTPVRGKVIQL
                     HETDGKRLEPVRYAVDRETLDTPMGKLETIVLTKQLDKNEDGGRDKKIWLAVDHHMLP
                     VRIIAHEKMGIVTDQMVTNISYGEPSNGLKQGQLQIK"
     gene            3895..5151
                     /locus_tag="VFI43_07580"
     CDS             3895..5151
                     /locus_tag="VFI43_07580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HET7062023.1"
                     /translation="MRLTPSYLDITTSALRAVLPLEFPADAILRNFFRNNPKLGPSER
                     AFIAQTIFGILRHRFFLESIILNPRPRSLLIAYLIKYGGLNLREVAPFLSERETKWAT
                     TLKAISEESRPLAIRAELPDWLIEKLRHFMTESAILALGRSLQQNASVDLRVNTLMAS
                     RENVIALLEQTGIASAATPYSPVGIRLAENPAINRHELFLSGKVEVQDEGSQLLGYLV
                     APKRRDMVVDFCAGSGGKALMLGAMMHSQGRIYAFDTSEKRLNNLKPRLKRSGLSNLF
                     PQLIAHEKDQKVKRLTGKIDRVLVDAPCSGLGTLRRNPDLKWRHSPQSIEELKHKQSA
                     ILSSAAHLLKPGGRLVYATCSFLPEENQQIVEDFLANNRQFTLLNCAELLAQQKIPVD
                     TGVFLQLSPASHGTDGFFAAAMEIKQ"
     gene            5576..6901
                     /locus_tag="VFI43_07585"
     CDS             5576..6901
                     /locus_tag="VFI43_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET7062024.1"
                     /translation="MQPESELHSLLRNLIDNVREITVLWQVAVLLASIGFAWLMQRQL
                     AKRMASRAASWEPAGLALNISPRSMSRLMFPIFGLVLLVIGRWALGHWYSTHLLNVAV
                     PLLFALALIRITVYVLRRVFSSQEWLHPWERFIGWAIWVGLALHITGLLPEILELLDE
                     ISFHIGKHRLSVLSILQGIVIFTVSMLLALWLASSLESRIMGSRTLEINQRVILSKIS
                     RTVLIVVGVLIALPMVGVDITVLSVFGGALGVGLGLGLQKIASNYISGFIILLDRSLR
                     IGDVVTVDNRKGEVTSLTTRYVVLKSDDGSDALIPNDTFITSTVVSQTYSNRKIRLTM
                     PFQVSYSSNVEVAMDIMLQAARKRPHILNDPEPVVFLKEFAGSGINVELIIWVEDPQG
                     GVLRLRSDLNIEIWAEFKKNGVDIPYPQYEVRLVTPDESAPDVNKAGKD"
     gene            7087..7353
                     /locus_tag="VFI43_07590"
     CDS             7087..7353
                     /locus_tag="VFI43_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062025.1"
                     /translation="MGALIFYTAIFFIGYFAAHFLNQITGRVLFQNRRIAGLALTLLV
                     GTLHGYKIITSSLPHDHENKAVYALGLYVILPIVIIGVAMLYLI"
     gene            complement(7397..7786)
                     /gene="gloA"
                     /locus_tag="VFI43_07595"
     CDS             complement(7397..7786)
                     /gene="gloA"
                     /locus_tag="VFI43_07595"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HET7062026.1"
                     /translation="MRILHTMLRVGNLEKSIAFYVDILGMRLLRSKDYPDGRFTLAFV
                     GYQDESEGAVLELTHNWDTDKYDMGTGYGHIAIEVDNAYQACEDVKKRGGKVTREAGP
                     MKHGATVIAFVEDPDGYKIEFIQKKTS"
     gene            complement(7802..7990)
                     /locus_tag="VFI43_07600"
     CDS             complement(7802..7990)
                     /locus_tag="VFI43_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET7062027.1"
                     /translation="MPTAETRVYRCYMCLICGFIYDEAEGSPAEGIPPGTRWEDVPMN
                     WTCPECAARKEDFEMVEI"
BASE COUNT         1936 a         2142 c         2094 g         1896 t
ORIGIN      
        1 agaaacgaca accaggtgag attgcgttga tccagccaat tttccaagcg cgcgatcttt
       61 ccttccctga agcagggaag accggaaacc ctacctgtaa actcaccgtc cttttgttcg
      121 ggctccgtcg caaccaggtt atctatcccc aatgcacgcg caatgggtcc cgtcacgaag
      181 ctgttcgttg cggtaatgat gatgcacaaa tcatggccaa gcatatgctg cttgatcagt
      241 tcatgtgcac ggggagcaat caatcgcaaa accctgttct tcatgaactc gctatgccac
      301 gcgtcgagtt gcacgcgggg atgccgtgac agcggcttca actgaaaatc cagaaattcg
      361 tgtatatcga gcgtgccggc cttgtattgc tcataaaatt ccagattgcg cgcttcgtat
      421 acttcacggt cgagcacgcg cttttcaatc aggaattggg cccattcgaa gtcgctgtcg
      481 cccgcgagga gggtattgtc gagatcgaat aaagcgagat tcacgattct tcctcaaata
      541 attcccggat caggggaacc gtaacttttc gctggctcgc caaggaatag ttgtcgagag
      601 catccagtat tgcaatcaat gaagacagat cccgctgtgt atgacgtaac aggtattcgc
      661 acacctcgtc cggcacatcg agtccccggc tggccgcgtg gcttttcatc gcctttattt
      721 tttcctcgtc ggtcaattcg tgaacctggt ataccagacc ccaaccgaga cgcgatacaa
      781 gatctgcacg catcttcagt tgcgcaggcg ggacggggcc gctcgcgagc ataaatgctc
      841 ggccttcatc atggatgtaa ttataaagac cgaatagccc gatctgcgaa tccgcaccga
      901 gatggtccac gccatccaca atgatgcaat cagcctcccg cccctcgacg aaacgagtgt
      961 cccgctggca ttcaaaatac gccaccttca ttttgctacg cctgcatatt gcggctattg
     1021 ctttcaacag atgtgtcttg ccggaacccg gcccacccca taaatagacg agacgttcgc
     1081 cctctcggcc cataacgacg ctgctcaggg tctgcagcaa ttcaatattg cgccccggca
     1141 cgaaattcgc cagcgtaggc tgcgcgggag gggcgatacc tagcagcaat tgcttcataa
     1201 gaaagcagaa ggttggcgct taccggatga tccgcccata tttacggtaa aatcagcgcg
     1261 attcaaaaaa ggaagcactc taactgcaac ttggcgagaa ctcaacttga cttcatccaa
     1321 atcggaaaac tcagcctcga atccgctttc ctaccgatca gcaggggtag atattgacgc
     1381 aggtgaccga ttggtggaaa acatcaagcc gtttgccaag cggacgatgc gatcggaagt
     1441 acttgcgggc atcggtgggt tcgccgcgtt attcgagatc tcgaggaact accggaaccc
     1501 cgttctcgta tccggtaccg atggtgtcgg gaccaaactc aaactcgcgt tcgtgtccga
     1561 caggcacgat agtatcggta ttgatctggt cgcgatgagc gtcaacgaca ttttggtgca
     1621 aggcgccgag cccctgtttt ttcttgatta tttcgcgtgt ggcacgctgg acgtatgcac
     1681 aggcacgaaa gtcattaaag gtatcgcgga agggtgcgaa caggcgggat gcgccctgat
     1741 aggcggggaa acggcggaaa tgccgggtat gtatccacgg ggtgaatacg accttgccgg
     1801 cttcgcagtg gggattgtgg aaaaaaaccg gatcgtgacc ggatcgacga tccatgaagg
     1861 agatgccgtt ctgggactgg cgtcaagcgg agcccattcg aacggttact ctctgatccg
     1921 caagatcatc gaaaaaaaca atatcgacct gtccgctgat ttcggcggac aagcgctgat
     1981 agacgtcctt atgaccccga cgcgcattta tgtaaaatcg atactcgagc tgatgaaaca
     2041 tcttccggta aagggcatgg cgcatataac cggggggggg ctgatagaga atattccacg
     2101 cgttttgccg gataatctgg cggcggtgct aaccctgaac tcatggcctg tgcccccgtt
     2161 gttccattgg ctccagcagc agggtaacgt agctgatcag gaaatgttcc gggtcttcaa
     2221 ttgcgggatt ggcatggtgc tcatcgtagc ggaagaattc gccggtgatg cgctgatgct
     2281 gttgcaatcc tccggagaga gcgcctggcg catcggccgg attgagccgc acgttgcaaa
     2341 ttcacctcga gcagtcattg aatgaaggga aacgcaagcg gttcccgcgc tgaccattga
     2401 agtccgttgt tatcctgatc tcaggccgcg gaagcaacat gcaagcgctg atggaagcga
     2461 ggctccctgc aagaataagc gcagtaatca gtaatcgacc caacgcaccg ggcctggcaa
     2521 tcgccaaaag gcatggaatt gaaaccatag cggtcgacca gcagtcatac cccgacaggc
     2581 gtgcattcga cgatgcgttg gcgaatacga tcgagggtta tactcctgac gtcattgcac
     2641 tcgcgggatt catgcatatt ttggataacg atctcgtcag ccgctatcaa ggtaaattgt
     2701 taaatgttca cccctcgctg ctacccgctt ttcccggcct cttctctcac cggcgggcat
     2761 tacaggaagg cgttaaaatc catggctgta ccgtgcactt cgttacgcct gagatggatc
     2821 gtggcccaat cgttatccaa gcggcaattc ccgtattgcc aggcgatacg gaggaaaccc
     2881 taggggcacg ggtactggaa caggaacacc gcatttatcc ggaagccgtg cgctggttcg
     2941 ttgaagaccg gctgaaactt tccggtaatt gtgtggacat aagcaaagcc gagcccgacg
     3001 gctcggtgct atattcacca ggattatcat catgaaaaaa tttcttgcag tcctcttctt
     3061 ttggctggcc ttccccgcca tctccgctct tcctgcgtct cgcggcaccg tcccatcgcg
     3121 cgtggatatc agttattcga tcacagcggg caaccttgaa ggcgaggtaa acgacacggt
     3181 ggaaatcagg caggaaaatg gcacccgaag ctacaacatt tacagcgagg ggcgagccaa
     3241 gggcttatta gcgctcactc aaccggataa cgtagtgaga gacagcgagg gaacggtcac
     3301 tcccaaggga gtcctgcgtc ccgcgcgctt ttctgaccag catggcaaaa aactgcccaa
     3361 ggttgccata tttgattggg acaaaaaatc gctaacgctt cagcacaaag gcaaggagga
     3421 acacagacaa ctgccggcgg atgcccagga caagttgagc ctgctataca gcttcatgtt
     3481 cactcctgtt cgtggcaagg tgatacagct tcatgaaaca gacggaaagc ggcttgaacc
     3541 ggtgcgatat gccgtggaca gggaaacact ggataccccg atgggcaaac ttgaaaccat
     3601 tgttctgacc aagcaattgg acaagaacga ggacggcggg cgcgacaaga agatctggct
     3661 cgccgtcgat caccatatgc tgcccgtacg tattattgcg catgaaaaaa tgggtattgt
     3721 gaccgaccag atggttacga atatcagcta cggagagccg tcgaacggct tgaaacaagg
     3781 gcaactccag ataaaataat ttgagctggc tccaataatt catctttcct gttgcgcacc
     3841 gtctccgagc gactcctgcg tggtagttgt gtgcctgata ttttgagcaa tccgctgcgc
     3901 cttaccccgt cctatctcga tatcaccact tcagctctgc gcgcagtgct tccgctggaa
     3961 tttccggccg atgcgatatt acggaacttc tttcgcaaca atccgaagct tggcccgagt
     4021 gaaagagctt ttatcgccca aaccatattc gggattctcc ggcatcggtt ttttcttgaa
     4081 agcataatcc tcaatccccg gccgcgctcc ctgctcatag cctatctaat caagtacggt
     4141 ggactcaatt tgagggaagt tgctcctttc ctcagcgaac gcgaaacaaa atgggcgaca
     4201 accctcaagg caatatcaga ggaatcgcga ccacttgcga taagagctga actcccggac
     4261 tggttgattg aaaagctgcg gcatttcatg acggaaagcg ccattctcgc actgggtcgc
     4321 tcattgcagc aaaatgcgtc cgtcgacttg cgcgtcaaca ctcttatggc aagccgggag
     4381 aacgtcatcg cgttgcttga gcagaccggg attgcgtcag ccgctacacc ctattctcct
     4441 gtggggatac gactggcgga aaaccccgcc attaaccgtc atgaactgtt cctgagcgga
     4501 aaagtggaag tccaggatga gggcagtcaa ttgctggggt atctggtcgc gccgaagcgg
     4561 cgcgatatgg ttgtcgattt ttgtgcgggg tccggaggta aggcgctgat gctaggagcg
     4621 atgatgcatt cgcaggggcg gatttacgct tttgatacat ccgagaagcg gctgaataac
     4681 ctgaaaccgc gcctcaaacg ctccggtctc tcaaatcttt ttcctcagct tatcgctcac
     4741 gaaaaagacc agaaggtgaa acgcttgact ggaaaaatag atcgagtcct ggtagatgct
     4801 ccctgtagtg gtctgggcac gctgcgccgt aatccggact tgaaatggcg tcactcaccc
     4861 cagagcatag aggaactcaa acacaagcag tctgcaatcc tctcttctgc tgcacacctg
     4921 ctcaagcccg ggggcaggct ggtttatgcg acctgtagct ttttgccaga ggagaaccag
     4981 caaattgtgg aggatttcct cgcgaataac cggcagttca ccttgttaaa ctgcgcggaa
     5041 ttactggcac agcaaaagat ccctgtcgat accggcgtat ttttacaact atcgcctgct
     5101 tctcacggga cggatggttt ttttgccgca gcgatggaga tcaaacagta aagcaggagc
     5161 gcgaaaaaca aaggcgggag ctcctccttc accttcttcg gccctgattt cttctactat
     5221 tggcgggcat cttgccgctt gcagcgaagc tgtcccaatc ggcgttccac tcaatagacc
     5281 ctgcctgcgt gctaccatgc gttagcataa acgaagacga gcatctcttc cccttgctga
     5341 tcagtcagta ggggtcgcac cttcccgaga tttatggtgg agaagatgtg cagacaaccg
     5401 gaatggcagc aagagcgctg tacggtttga tgcgaatagc gctttccgcc gcgtagacag
     5461 acgtctggca tggcagaagt tgcgcttcag tggtccggat tcccccgaag tctgtaacgg
     5521 caccaaaaaa cccaattcaa tacgccgtag caagcagaaa tgctgagaat tctcaatgca
     5581 acctgaaagt gaattgcaca gtctgcttcg caatctgatc gacaatgttc gtgaaattac
     5641 tgtgctgtgg caagtggcgg ttttgcttgc cagcatagga tttgcgtggc tcatgcagag
     5701 gcagctagcc aagcgtatgg cgtcacgtgc agcatcctgg gaacctgcag gactcgcgct
     5761 caatatcagt ccgcgaagca tgagtcggct catgttccct atcttcggcc ttgttctgct
     5821 cgtcatcggc aggtgggcgc tcggccactg gtatagcacc catcttctga atgtcgcggt
     5881 ccccctgcta ttcgccctcg cgttgattcg tatcaccgtc tatgtgctcc gaagggtttt
     5941 ttctagccag gaatggctgc atccctggga gcggtttatt ggttgggcaa tatgggtcgg
     6001 cctggcgctc cacattaccg ggttgttgcc ggaaatcctg gagcttctcg acgaaatttc
     6061 ctttcacatc ggaaagcatc gcctctccgt gctatcgatt cttcaaggga tcgttatctt
     6121 cacggtcagc atgctgcttg ccctatggct cgccagttcg ctcgaatccc gcatcatggg
     6181 ctcgcgaaca ttggagatca accagagagt cattctgtca aaaatcagtc gcacggttct
     6241 gattgttgtc ggtgtgctga tcgccctgcc catggtgggt gtcgacataa ccgtgctgtc
     6301 ggtattcggc ggcgcgcttg gggtaggact gggattgggt ctgcagaaaa tcgccagtaa
     6361 ctatataagt ggattcatca ttctgctgga tcgctcacta cgcatcggcg acgtcgtaac
     6421 tgtcgataac cggaaagggg aagttacctc ccttacaacg cgctacgtag tactcaagag
     6481 cgacgacggt agcgatgcac tcatccccaa tgacaccttc atcacctcga cagtcgtcag
     6541 ccagacttac tcgaaccgca aaatccgtct gacaatgccc ttccaggtca gttactccag
     6601 caatgtcgag gttgcaatgg atatcatgct gcaagcagca aggaaacgtc ctcacatatt
     6661 gaacgaccct gaacccgttg tcttcctcaa ggagtttgcc ggtagtggga tcaacgtaga
     6721 actgatcata tgggtagagg acccccaggg cggagtgctg aggctacgat ctgaccttaa
     6781 catcgaaatc tgggcagagt tcaagaaaaa cggggtagac ataccttatc cccagtatga
     6841 agtgcgattg gtgacgcccg atgagagtgc gcctgatgtg aataaggcgg gaaaagacta
     6901 aaaagaagag aaggccaaaa gtaagagagg tctaaaaggg agagaacctg atattttcgg
     6961 ttcactcgac agaactttat gctatcgcca tcgagaatct atctcacacc gcaccgattc
     7021 ggggaaggag taagcaagat tccgactgga gcgttgtaat gattggcagt aaaatccgaa
     7081 gaacctatgg gtgccttgat cttttatacc gcgattttct ttatcggtta tttcgcggcg
     7141 cactttctca atcaaataac cggccgcgtc ctttttcaaa atcgcagaat tgctggactc
     7201 gccctgaccc ttttagtcgg cacgcttcac ggctataaaa taataacgtc cagcctgcct
     7261 catgatcacg aaaacaaggc cgtttatgcg ctcggtctat atgttatttt gcctatcgtg
     7321 attatcggcg ttgcgatgtt atacctgata tgacaggaaa agcgtgatga cagctctaac
     7381 tgatcagtcc tgacattcaa gatgtctttt tctggatgaa ctcgatcttg tagccgtcag
     7441 ggtcttcaac aaacgcgatc acggtcgccc cgtgcttcat tggtcccgct tcccgcgtaa
     7501 cctttcctcc gcgttttttc acgtcttcac aagcctggta ggcgttatcc acctcaatcg
     7561 cgatgtgccc ataaccggtt cccatatcat acttgtcggt atcccaatta tgggtcagtt
     7621 ccagcaccgc gccctctgat tcatcctggt atccgacaaa cgcaagggta aacctgccgt
     7681 cagggtagtc cttgctgcga aggagcctca tcccgaggat gtctacataa aaagcgatgg
     7741 atttttccag atttccgacg cgcagcatgg tatggaggat tcgcatcaga tttccgtcgt
     7801 cttagatttc caccatttca aaatcttcct tccgggctgc acattcggga caggtccagt
     7861 tcattggcac gtcttcccat cttgttcccg gggggatacc ttccgctggt gacccttcgg
     7921 cttcgtcgta tatgaagccg caaatcaggc acatgtagca acggtaaacg cgagtttcag
     7981 ctgttggcat gatgagtgag agtaatatgg gttaaaatgg cattttacgg tatgaaccca
     8041 tgtctcagcc accccccatt gtactttc
//
LOCUS       DASSHJ010000195         7024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1219892,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000195 DASSHJ010000000
VERSION     DASSHJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219892"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFI43_07605"
     CDS             complement(<1..660)
                     /locus_tag="VFI43_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET7062028.1"
                     /translation="MIKILRLWHYLTRYVDGLLLMGILALMLTGLITLFSATDANLAR
                     VTNQATNMLVALGIMWLVANIPLQHIMRIALPIYATGLVLLLGVMLFGEINNGARRWL
                     NIGVTRIQPSELMKIAVPLMLAWYFDKHETTLRLRDYGVAALLLLLPVVMILRQPDLG
                     TALLIAASGFYVLFLTGLSWRIMAALFVLGAGSLPLMWSMMHDYQRRRVMTLLDPTQD
                     AL"
     gene            complement(653..2590)
                     /gene="mrdA"
                     /locus_tag="VFI43_07610"
     CDS             complement(653..2590)
                     /gene="mrdA"
                     /locus_tag="VFI43_07610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7062029.1"
                     /translation="MKGAVELRNHLRELRNFQLRLAITAVFVLVMCLMLFARFYYLQV
                     MQREHYHTLAEANRISIAPIVPNRGLIFDRNGEVLAHNYSAYTLEIVPSKVVDSDLET
                     LINELSTVVEIAPRDRKRFKKLMEESKRFESLPIRTRLSDVEVARFAANRYRFPGVEI
                     KARLFRQYPKGESASHVVGYIGRINDKDLEQLEANDDLPNYRGSQYMGKIGVEQSYEK
                     ELHGITGFEEMETDAAGRVIRVISRTPPTSGNNLMLSLDSKLQEVAEKAFGERRGALV
                     AIEPSSGDVLAFVSKPGFDPNLFVDGIDSENWDLLNNSIDRPLNNRALRGMYPPGSTF
                     KPFMALAGLELKKRVPQQAINDAGFFSLPGSSHRFRDWKAGGHGVVDLHKSLVVSCDT
                     YYYGLANDLGIDNIFSFGSRFGLGRKTGIDIEGEVAGLLPSQDWKMKRHKQKWYAGDT
                     ISVGIGQGYNLATPLQLAFATAILAGNGTAYRPHLVKKVLDNNNQVVREIATEPLYSL
                     NLNAANVATVRNALIDVARPGGTAAVAGAGAAYTLAGKTGTSQVVGIKQGEKYVENKI
                     QERHRDHALFIAYAPAENPKIAVSVLVENGGHGGSSAAPIARLVMDYFLLGKLPEESA
                     AKTVEASGDEEEESVEADSHD"
     gene            complement(2587..3108)
                     /gene="mreD"
                     /locus_tag="VFI43_07615"
     CDS             complement(2587..3108)
                     /gene="mreD"
                     /locus_tag="VFI43_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7062030.1"
                     /translation="MAFVNQTKQEILLPAKGSFIILSLLFALVLNLLPLKGMALALWP
                     DFVALVILYWCINQPQRAGISMAFGMGLLMDIGDASALGQNALAYTVMAFLGLVFQRR
                     LSVFGPAKQAPQIGLILFAGQLILLFTGLLDGSHFPGWNFFLVTIISMLLWPLVSSLL
                     RMPQKPRSDSNAG"
     gene            complement(3095..3988)
                     /gene="mreC"
                     /locus_tag="VFI43_07620"
     CDS             complement(3095..3988)
                     /gene="mreC"
                     /locus_tag="VFI43_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7062031.1"
                     /translation="MAPRFFRHGPSLLGRLIFFVMLSLVLMAIDTRFKYLVEIRQVVS
                     TATYPLQKLANAPSALYGQLSEYFSGLDLVDENEKLKQQHLVDSGQLQRLRALEAENA
                     QLRNLLETAQRIESKAVMAEILHVPRDPFNRKVMLDKGSQGGIQPGQVVVDEAGVVGQ
                     VTRTYPWLSEVTLITDKDQSVPVQVVRNGLRSVVSGTGKDGALELRYAAVNTDIQEGD
                     LLTTSGIDGVYPPGLPVAVVSKIERNPSYVFARVTCLPAGGVARNRQLLILSLMPAVE
                     IPPEALTIKPEKKRREKGGIR"
     gene            complement(4069..5127)
                     /locus_tag="VFI43_07625"
     CDS             complement(4069..5127)
                     /locus_tag="VFI43_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7062032.1"
                     /translation="MLNFINNNVLKGYFSTDMAIDLGTANTLIYVRGQGIVLNEPSVV
                     AIRQDGGPNGKKVIQQVGLAAKQMLGRTPGNITAIRPMKDGVIADFTVTEQMLKHFIK
                     KVNPPRLFSANPRIVICVPYGSTQVERRAIREAAYGAGARKVELIEEPMAAAIGANLP
                     VEEATGSMVVDIGGGTTEVGVISLGGIVYSNSVRVGGDKFDEAIINYIRRNYGMLIGE
                     VTAELIKKEIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTDPLNSIVSA
                     VKSALEHTLPELGADIAEKGMVLTGGGALLRDIDRLLMEETGLSVIIAEDPLTCVVRG
                     SGIALENMDQLVGIFASD"
     gene            5252..5539
                     /gene="gatC"
                     /locus_tag="VFI43_07630"
     CDS             5252..5539
                     /gene="gatC"
                     /locus_tag="VFI43_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7062033.1"
                     /translation="MSLSLDDVNRLANLARIELGKDEASTALAQLSDIFGMIEQMQAV
                     NTSEIAPMSHAQDVTQRLRDDVVTEPDQRSLFQAIAPQVEAGLYLVPRVIE"
     gene            5614..>7024
                     /gene="gatA"
                     /locus_tag="VFI43_07635"
     CDS             5614..>7024
                     /gene="gatA"
                     /locus_tag="VFI43_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7062034.1"
                     /translation="MLNLSLKQLSALLAERKISSVELTSEFLKRSHTLNQRYNAFITF
                     NEEISLAQARAADAAIATGQAGPLTGIPIAQKDIFCTKGWLTTCGSKMLSNFISPYDA
                     HVVERFNAAGTVTIGKTNMDEFAMGSSNETSFYGSVKNPWDTSAVPGGSSGGSACAVA
                     ARMVPAATGTDTGGSIRQPAALCGISGLKPTYGLVSRYGMIAFASSLDQGGPMAKSAE
                     DLALMLNVMAGFDTRDSTSLMREAEDYTRDLEKPLEGLRIGLPKEYFVEEINDDVARS
                     VEAAIGEYRKLGAETVEVSLPNTPLSVPVYYVLAPAEASSNLSRFDGVRYGYRAPEYS
                     DLNDMYRKTRAQGFGAEVKRRILIGTYVLSHGYYDAYYIQAQKLRRLMAHDFSEAFKQ
                     CDVIMGPTSPTVAFNLGERISDPVQMYLSDAYTIAVNLAGLPAMSIPSGFGSKGRPVG
                     LQIVGNYFSEAKMLNVAHQY"
BASE COUNT         1555 a         1986 c         1927 g         1556 t
ORIGIN      
        1 cagcgcatcc tgggttggat ctagcagcgt catcacccgc cggcgctggt aatcgtgcat
       61 catcgaccac atgagcggga ggcttccagc tcccagcacg aaaagcgccg ccatgatgcg
      121 ccatgataac cctgtaagaa aaagcacgta gaagccgctc gcggcgatca gcaaagccgt
      181 gccgagatcg ggctggcgca ggatcatcac caccgggagc aggagcagca gtgcggcgac
      241 cccgtaatct cgcagccgaa gcgtggtctc atgtttgtca aaataccatg cgagcatcag
      301 cggcacggcg attttcatga gttcggaggg ctgaatacgc gtaactccga tattcaacca
      361 acggcgggcg ccattgttga tctcgccgaa gagcatgacg cccaaaagca ggaccaggcc
      421 ggtcgcatag attggcagag caatccgcat gatgtgctgt agggggatat tcgcaacaag
      481 ccacatgatg cccagcgcga ccagcatgtt cgtggcctga ttggtgacgc gggccaagtt
      541 ggcgtcagtc gcgctgaaca gcgtgatcaa gccggtcagc atcagcgcca ggatgcccat
      601 caggagcaat ccatccacgt aacgcgtcag gtaatgccaa aggcgaagaa tcttaatcat
      661 gagagtctgc ttccaccgat tcttcttcct catccccgct ggcttcaacc gtttttgcgg
      721 cagattcctc ggggagcttg cccagcagga aatagtccat caccagcctt gcgataggcg
      781 ctgcacttga accgccatgc ccgccatttt ctaccagtac cgacactgcg attttcggat
      841 tttccgccgg tgcgtaagca atgaacagcg cgtggtcgcg atggcgttcc tgtatcttgt
      901 tctccacgta tttttcaccc tgcttgatac cgaccacctg agaagtgcct gtcttgccgg
      961 caagcgtata ggccgcgccc gcgccagcta ccgctgctgt tccgcctggc cgggcgacat
     1021 cgatgagcgc gttgcgcacg gttgcaacgt tagccgcgtt gagattgagc gaatacaaag
     1081 gctccgtagc aatctcgcgg actacctggt tgttattgtc cagaaccttc ttgaccagat
     1141 gcggacggta agcagtgcca tttccagcga ggattgccgt ggcgaatgcg agctgcagcg
     1201 gagttgccag attgtaacct tgtcctattc cgacagagat ggtgtctccc gcataccact
     1261 tttgcttgtg ccgcttcatt ttccaatcct gcgacgggag caagccagcg acctcgcctt
     1321 ctatatcgat tccggttttc cttcccagcc cgaaccggct tccaaagctg aaaatattat
     1381 caatgcccag gtcgttggcg agcccgtagt aataagtgtc acacgacacc accaaagact
     1441 tgtgcaggtc caccactcca tgacctcccg ctttccagtc gcggaagcga tgagaacttc
     1501 cgggcaggct gaaaaacccc gcatcgttga tggcctgctg gggtacgcgc ttttttagct
     1561 ccagccccgc cagcgccatg aaaggcttga acgtggagcc aggcggatac atgccacgca
     1621 aggcgcggtt gttgagcggc ctgtcgatgg aattattcaa taagtcccag ttctccgaat
     1681 caataccatc gacaaaaagg ttggggtcga agcccggttt gctgacgaac gcaagtacgt
     1741 cgccggagga aggctcaatc gccaccagcg cgccacgccg ttctccaaaa gccttttccg
     1801 cgacttcctg cagtttcgag tcgagcgaaa gcatcaggtt gttgccggag gtcggggggg
     1861 tgcgggatat cacgcggata actcgccccg ccgcatcggt ttccatctcc tcgaagcctg
     1921 ttatgccgtg cagttccttc tcgtaactct gctcgacccc gatcttcccc atgtattgcg
     1981 agccccgata attcgggagg tcgtcgttcg cctcgagctg ctccagatcc ttgtcattta
     2041 tgcggccgat gtatcccacc acgtgggaag cactttcccc ttttggatat tggcggaaca
     2101 gccgggcctt gatttccact ccggggaatc gataccggtt ggctgcaaaa cgcgcgacct
     2161 cgacgtccga taagcgcgtc cgaatgggca ggctttcgaa gcgtttgctt tcttccatca
     2221 acttcttgaa acgcttgcga tcccttggtg cgatctcgac tacagtggaa agctcgttga
     2281 tgagcgtctc aagatcggaa tcgaccacct tgctggggac gatctcaagc gtatatgcgg
     2341 aataattgtg cgccagcact tcgccgttgc ggtcgaagat caggccgcga ttggggacga
     2401 tgggtgcaat cgagatgcgg ttggcttcgg ccagggtgtg atagtgctcc cgctgcatga
     2461 cctgtaaata atagaaacga gcgaacagca tgaggcacat cactaatacg aacacagcag
     2521 tgatggcaag ccgcaactga aaattgcgca gttcgcgtag atggttacga agctctactg
     2581 cgcctttcat cctgcatttg agtcagacct aggcttttgc ggcattctca gcagcgaaga
     2641 aaccagaggc cacagaagca tgctgattat tgtcaccaga aaaaaattcc agccgggaaa
     2701 atgtgaccca tccagcaatc ccgtgaaaag caggatgagt tggcccgcaa acagtatcag
     2761 gccgatctgg ggagcttgct ttgctggccc gaagacgctc aggcgccgct ggaacaccag
     2821 acccaggaat gccatgacgg tatacgcaag tgcgttttga cccaacgcgc tcgcgtctcc
     2881 gatatccatt aacaatccca ttccgaaagc catgctgatg cccgcgcgct gcggctggtt
     2941 gatgcaccag tagagtatga ccagggcgac gaagtctggc cagagagcaa gggccatgcc
     3001 tttcaagggc agcagattca gtaccagtgc aaaaagcagg cttaagatga tgaaagagcc
     3061 ctttgcgggc aggagaatct cctgctttgt ctgattaacg aatgccaccc ttttctcgcc
     3121 tctttttctc gggcttgatg gtcagggctt ctggcggaat ctccactgcc ggcatcagcg
     3181 acagtatgag aagttgccgg ttacgtgcta ctccaccggc aggcaggcag gtaacgcgag
     3241 caaacacata ggatggatta cgctcgattt ttgagaccac ggcgaccggc aggcccggcg
     3301 gataaacacc gtctattccc gaagttgtca gcaggtcccc ctcctggata tccgtattta
     3361 cggcagcata ccgcagttcc agcgcgccat ccttgccggt gcctgatacc actgatcgca
     3421 acccattacg cactacctgt acagggacag actggtcctt gtctgtgatg agggtgactt
     3481 cggatagcca aggataggtg cgcgtgacct gccctactac ccccgcctcg tccacgacga
     3541 cctgccccgg ctggataccc ccctggcttc ctttatcgag cattaccttc cgattgaacg
     3601 gatcccgtgg aacatggaga atttcagcca tgacagcctt gctttctatc cgctgcgccg
     3661 tttccaggag attgcgcagc tgggcatttt cggcctccag tgcccgcaag cgctgaagct
     3721 gtccactatc gaccaagtgc tgctgcttga gtttctcatt ttcgtcaacg agatccaggc
     3781 cggagaaata ttcgctcaac tggccataca gtgcggatgg cgcattcgcc aatttttgca
     3841 atggataggt tgccgtagag actacctggc gtatttcgac gagatacttg aagcgggtat
     3901 cgatagccat cagcaccaat gacagcatta caaagaaaat caggcgcccg agcaggctgg
     3961 ggccgtgcct gaaaaacctt ggagccattt cagtcacaca cgaggttccc ggatttccgg
     4021 ttcagcaagt accggatgga aagcggaccg gcaaggtgga aaaaaccatc agtcactcgc
     4081 gaatatgccg accaattgat ccatgttctc caaggctatg cctgaccccc ggacgacgca
     4141 ggtaagcggg tcttctgcga tgatgacgga caggccggtt tcttccatca gcaagcggtc
     4201 gatatcgcga agcaacgcgc cgccgccagt cagtaccatg cccttttccg cgatatcggc
     4261 ccccaactcg ggcaatgtat gctccagggc ggacttgacc gcacttacaa tgctgtttaa
     4321 cggatcggtg agggcttcaa gaatctcgtt gctggaaatg gtgaagctgc gtgggatgcc
     4381 ttcggcgagg ttacgtccct tgacttccat ttcgcgaacc tctgaacccg ggaaagccga
     4441 tcctatttcc tttttgatca attcagcggt aacttcgccg atcagcatgc cgtagttgcg
     4501 gcggatatag ttgatgatgg cctcgtcgaa cttgtcaccg ccgactctca cagaattcga
     4561 ataaacgatg cctccgagcg agatcacgcc aacctcggtc gtgcctccac ctatatcgac
     4621 gaccatcgag cctgtggcct cctcgaccgg aaggttggcg ccaattgcgg ccgccatggg
     4681 ctcttcgatc aattcgacct tgcgagcccc cgcgccatat gcggcttccc gaatggcgcg
     4741 ccgctcgacc tgcgtggatc cataaggaac acaaatgacg attcgcgggt tggcggaaaa
     4801 caggcgcggc ggattcacct tcttgatgaa atgcttcagc atctgctcgg taacggtaaa
     4861 atcagcgatc acgccgtctt tcatgggccg gatcgcggtt atgttgccgg gggtgcgccc
     4921 gagcatctgc ttcgccgcca gcccgacctg ctggatgacc ttcttgccgt tgggcccccc
     4981 gtcctggcgg attgcgacca ctgagggctc gttcagcacg atgccttggc cgcggacgta
     5041 gatcagggtg ttcgctgttc ccagatctat tgccatatcg gtcgaaaaat agcccttcaa
     5101 cacattgttg tttatgaaat taagcatgat ttacggaatc cgtcgctgtg gcgagagaaa
     5161 gctgatggta aagcgggaaa agatgcggta gtcaggcgcg ctatgatact ctattagcta
     5221 cttgaatata agggttatag gttgagtccg catgtcactt tcgctggatg atgtaaaccg
     5281 ccttgcaaat cttgcccgca ttgaactcgg aaaggatgaa gcgtccactg ctcttgctca
     5341 gctatcggat atctttggaa tgattgagca gatgcaggct gtgaatacgt cggaaattgc
     5401 gccaatgtcg catgctcagg atgtaaccca gcgcttgcgc gatgacgttg tgaccgaacc
     5461 cgatcagcgt tcactcttcc aggcaattgc accccaggtc gaagcagggc tttacctggt
     5521 gcccagagtc atcgagtaga gcgccgcaac ccgcctccgt tgcaggctgt tcgcttattt
     5581 cccgatcgat ttgaacccct cgccctgagc ctcatgttga atctcagcct taaacagcta
     5641 tccgcactgc tcgcagaaag aaaaatttcg agtgtcgagt tgacctcgga atttctcaag
     5701 cgttcacata cgctgaacca gcgctacaac gccttcatta ctttcaacga ggaaataagt
     5761 ctcgcgcagg cgcgtgcagc cgatgcagcg attgcaaccg ggcaagccgg ccctttgacg
     5821 gggataccta ttgcccagaa ggacatcttt tgcaccaagg gatggctgac cacttgcgga
     5881 tcgaaaatgc tctcgaactt catttcgccc tacgatgcgc atgtggtgga gcgtttcaat
     5941 gcagctggaa cggtgaccat aggcaagacg aacatggatg aatttgccat ggggtccagt
     6001 aacgaaacct ccttttacgg ttcggtaaaa aatccgtggg atacttcagc cgtgccgggc
     6061 ggaagctccg gtggttcggc ttgcgcggtg gctgctcgca tggttcccgc tgcaaccggt
     6121 accgacaccg gtggctcgat ccgtcagcca gcggcgctgt gcggtatttc cggcctcaag
     6181 ccgacctacg gactggtatc acggtacgga atgatcgcgt tcgcttccag cctagaccag
     6241 ggagggccga tggcaaaatc cgcggaagac ctcgcattga tgctgaacgt gatggcggga
     6301 tttgacacgc gtgactcgac cagcctcatg cgtgaggcgg aagattacac gcgcgatctg
     6361 gaaaagcccc tggaaggcct gcgtattggc ttgcccaagg aatatttcgt cgaggaaata
     6421 aatgatgacg tggcacgctc ggtagaagcc gcgatcgggg aataccgcaa gctcggggcc
     6481 gaaacggtcg aagtgtctct ccctaatacg ccactatcgg ttccggttta ttatgtgctt
     6541 gcgcctgcag aagcgtcgag taatctttcc cgtttcgacg gcgtgcgtta tgggtatcgg
     6601 gcaccggagt attcagacct gaacgatatg taccgcaaga cccgcgccca gggtttcggc
     6661 gcggaggtca agcggcgcat cctgatcggg acgtatgtgc tctcgcatgg ctactatgac
     6721 gcatattaca tccaggcaca gaaactgcgg cggctgatgg cgcacgattt ttccgaggct
     6781 ttcaagcagt gcgacgtgat catgggcccg acctcgccga ctgtggcatt caatcttggc
     6841 gaaaggatca gtgatcccgt acaaatgtat ttatcggatg cctataccat cgcagttaat
     6901 ctggccggtt tgcccgcgat gtctattcca tcgggattcg gcagcaaagg caggcccgtg
     6961 ggattgcaga ttgtgggcaa ttatttctcc gaagcaaaaa tgctgaacgt ggcgcaccag
     7021 tatc
//
LOCUS       DASSHJ010000196         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_882258, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000196 DASSHJ010000000
VERSION     DASSHJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882258"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /gene="rsmH"
                     /locus_tag="VFI43_07640"
     CDS             <1..575
                     /gene="rsmH"
                     /locus_tag="VFI43_07640"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7062035.1"
                     /translation="DMSEGPTAADWLASVTETELEEVLRKYGEERFAKQIARAIVAVR
                     GAEPIITTGQLAALVARVIPVHRREAGQNPATRTFQAIRIYLNQELENLSLALPQCAE
                     MLNPGGRLVVISFHSLEDRIVKRFLRKLASPDVMPRRLPLRALEVQRYAQQKMRLVGK
                     PIRPGAHEIASNPRARSAVMRVAERVDMVH"
     gene            630..935
                     /gene="ftsL"
                     /locus_tag="VFI43_07645"
     CDS             630..935
                     /gene="ftsL"
                     /locus_tag="VFI43_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET7062036.1"
                     /translation="MTRINILLIFIFIACALSIVSSQHKARRLFVDLEKAQELASRLA
                     VEWDQLQLEQLTWATHARVEKIAVNQLNMRILDASRVQTLPLQESTVPAPLVPGSKP"
     gene            932..2665
                     /locus_tag="VFI43_07650"
     CDS             932..2665
                     /locus_tag="VFI43_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7062037.1"
                     /translation="MTSRLPPRVILPSGRARLLIVVLLLAMVSLIGRAAYLQGMRNGF
                     LQEKGESRHSRVLEMSAHRGMITDRHGEPLAISTPVESVWASPKDMNATAEQLEKLAA
                     LIGVDAAELRKRIDTSQGGTQRDFVYLKRHLPPEAAARVVELDIKGVFLKREYRRYYP
                     SGELMSHMLGFTDIDDNGQEGIELALQDKLAGKPGSRRVIRDRKGYIVEDVENIRAPR
                     PGQNVALSIDSKIQYLAYRELKQAVEANKAKAGGIVVLDVRTGEVLALVNLPTYNPNN
                     RAGRGDNQFRNRALTDVFEPGSTLKPFTIAAALEAGGIKPDTVFQTAPGTFSIGRATI
                     RDAHKENALTVAQVIQKSSNIGSAKIALSLPSQTLWEMLSDAGFGAPTGSGFPGEVSG
                     KLRPYRSWRPIEQATMSYGHGIAVSLLQLARAYTLFSSGGELKPVSLLKLDALPEGKR
                     VISQDTAMAVSEMLEMVTQLGGTAPQARIEGYRVAGKTGTAHKPEGNGYAKNRYLSTF
                     VGYAPASNPRFIIAVMLDEPSAGKYYGGAVAAPVFSRVMAGTLRLNNVPHDGSGKVMS
                     FTAAPATKGDV"
     gene            2662..>3826
                     /locus_tag="VFI43_07655"
     CDS             2662..>3826
                     /locus_tag="VFI43_07655"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7062038.1"
                     /translation="MSPLLNKEDWDYPGSGDFDFALLEKLGVEIRNLVTDSREVKPGD
                     TFLAYPGEKSDGRRYIPQAIAAGVSSILWDSAGFAWNSAWHIPNLPVAGLRAKAGIIA
                     DQVYRHPSSKLWLIGITGTNGKTSCSHWIAQAMTALGTRAAIIGTLGIGFPGELVPTA
                     NTTPNAVLLQKELAELFERGARVAAMEVSSHGIVQERINGSTFGVALLTNLSRDHLDY
                     HGSMEAYAEAKARLFRWPGLKHAVLNMDDPFGVQVSRNLAGASVEIIGYGFTDFLNHI
                     PVAGYAKNMRVMRGYGVKSDPEGLMFNIEFESEKIEFKAGVIGGFNASNLLGVLATLI
                     TSGINLREAVEALRAIRPVAGRMEQMGGGDSPLIVIDYAHTPDALEKVLMTVRE"
BASE COUNT          889 a          981 c         1161 g          795 t
ORIGIN      
        1 tggatatgag tgaggggccc acggccgctg actggctggc ctccgttact gaaacggaat
       61 tggaagaggt attgagaaag tatggcgaag agcggtttgc taaacagatt gcaagggcga
      121 ttgttgcagt tcggggcgcc gagcccatta ttacgacggg tcagctcgcc gcgctcgtgg
      181 cgcgggttat ccccgtacac aggcgcgaag ccgggcagaa cccggcgacg agaacttttc
      241 aggccatacg gatttatctc aatcaggagc ttgaaaatct ctcgctggca ttaccgcagt
      301 gcgctgaaat gctgaaccca ggcggccggc tcgtcgtcat cagctttcac tcgcttgagg
      361 acaggatagt caaacgtttc ctgcgcaaac tggcaagtcc ggatgtcatg cccaggcgat
      421 tgccgctgcg agccctggaa gttcagcgtt acgcacagca gaagatgcgg ctggtgggga
      481 agccgatacg tccgggggca catgagattg ccagcaaccc acgtgcacga agcgcagtga
      541 tgagggtagc ggaacgcgtt gacatggttc attgacgacg ccgatatggg acgaacgata
      601 tttattttgt gtgcagcccc ggggctgatg tgacccgtat aaacattctt ttgattttca
      661 tttttatcgc ctgcgcgctg agcatcgtgt cgtcgcagca caaggcgaga aggcttttcg
      721 tcgatctcga gaaagcgcag gagctagcga gccggctggc cgtggaatgg gaccagctgc
      781 aactcgagca acttacctgg gcgacgcatg cgcgcgtcga gaagatagcc gtaaaccagt
      841 tgaacatgcg gatactagat gcttcgcgcg tccagactct tccattgcag gagtcgaccg
      901 tccctgctcc tttggtcccc gggtccaagc catgacgagc cgcttgcctc cccgcgtcat
      961 ccttccctcc ggccgcgcgc ggctattgat cgttgtatta ctgttggcaa tggtctcatt
     1021 aattgggcgg gccgcgtact tgcagggaat gcgtaacggg tttctgcagg agaagggcga
     1081 gtcgcgccat agccgcgttc tcgagatgag tgcccatcgg ggaatgatta ccgatcggca
     1141 tggcgaaccg cttgctatca gcacccctgt cgagtcggta tgggcaagcc cgaaagacat
     1201 gaatgcgacg gctgagcagc tcgagaagct tgcggctctg ataggcgttg acgcggcgga
     1261 attgcgaaag cgcatcgata cgtcacaagg cggaacccag cgcgattttg tgtatctgaa
     1321 gcggcatctc cctccagagg cggcagcaag ggtggttgag ctcgacataa agggcgtttt
     1381 cctcaaacgc gaataccgcc gttattaccc ctcgggagag ctcatgtccc atatgttagg
     1441 gtttaccgat atcgatgaca acggccagga aggcatcgaa ctggcgctgc aggataaact
     1501 cgcgggcaag ccaggaagcc ggcgcgtcat ccgggatcgg aagggatata ttgtcgagga
     1561 cgtggaaaac atacgcgcgc ccagaccggg acaaaacgtt gccttgtcca ttgacagcaa
     1621 gattcagtat ctcgcgtacc gggagttgaa gcaggcagtg gaagcaaaca aggccaaggc
     1681 gggtggaatc gtggtgctcg atgtacgcac aggtgaagta ctggcccttg tcaatctacc
     1741 tacttacaac ccgaataacc gggctggaag gggtgataac cagttccgta acagggcgtt
     1801 gacggatgtg ttcgagcctg gctcgacact taagccgttt acgatagctg cagctctgga
     1861 agcaggcggg ataaagccgg acaccgtctt ccagacagca cccggaacat tttccatagg
     1921 cagggcgaca atacgtgatg cccataagga aaatgcattg accgtagcgc aggttatcca
     1981 gaaatccagc aatatcggct ccgcaaagat cgcactttca ctgccttcgc aaaccttgtg
     2041 ggagatgctg agtgatgccg ggttcggggc gccgacgggc tcgggtttcc cgggagaagt
     2101 cagcggaaag cttcgcccct atcggagctg gcgtcccatc gagcaggcaa ccatgtcata
     2161 tggtcacggc attgccgtga gtctcctgca acttgcccgt gcgtacacgt tgttttcctc
     2221 aggcggagaa ctgaagcctg tgtcattgct gaagcttgat gcgctgccgg agggcaagcg
     2281 cgtgatttca caagataccg caatggctgt aagcgaaatg cttgaaatgg tcacccagtt
     2341 gggaggcact gcacctcagg cccgcataga gggttatcgg gtcgcgggca agacggggac
     2401 tgcacataaa cctgaaggta acggctacgc aaaaaatcgc tatctctcca cattcgtggg
     2461 gtacgcgccc gcgtccaatc cccgcttcat tatcgcggtc atgctcgatg agccttccgc
     2521 cggtaaatat tatggaggag cggtggcggc gcccgtattc agccgggtca tggctggaac
     2581 gctgcgtttg aacaatgttc cccacgacgg ctcaggaaag gtgatgtcat ttacggcagc
     2641 accggctacg aagggggatg tatgagcccc ttgctcaaca aggaagactg ggattacccg
     2701 gggagcgggg attttgattt tgcactcctt gagaagcttg gggtggagat caggaacctt
     2761 gtcacggata gccgcgaagt aaagccggga gacacttttc tggcctatcc aggcgaaaag
     2821 tccgatggcc gcaggtatat accccaggca atcgcagcag gagtttccag catattatgg
     2881 gacagcgcag gttttgcatg gaattccgcc tggcatatcc cgaatttgcc ggttgccggg
     2941 ctgcgcgcca aagcgggcat tatcgctgat caggtatata ggcatccatc aagcaagtta
     3001 tggttgatcg ggattaccgg aacgaacggc aagacctcct gcagccactg gatagcacaa
     3061 gccatgaccg cgcttggcac cagggctgcg attatcggca ccttgggaat cggtttcccg
     3121 ggtgaactcg tcccaactgc gaacacgacc cccaacgcgg tattgctcca gaaagaactg
     3181 gcagagcttt tcgaacgcgg cgcgcgcgtc gccgcgatgg aagtctcatc gcacggcatc
     3241 gtgcaagagc gcattaacgg gagcacattt ggagttgcgt tgctcacgaa tctttcgcgc
     3301 gatcacctgg attatcatgg gagcatggag gcgtatgccg aggcgaaagc gcggctgttt
     3361 cgctggcccg gactcaagca cgcggttctc aacatggatg atccatttgg tgttcaagta
     3421 tcgagaaacc tcgcaggcgc gagcgtcgaa ataatcggat atggatttac cgacttcctt
     3481 aatcatatcc cggttgcggg atatgctaaa aatatgcggg tgatgcgcgg gtacggcgtt
     3541 aaatcggatc cggaaggatt aatgttcaat atcgaatttg aatccgagaa aatcgaattc
     3601 aaagcaggag tgataggagg tttcaacgca tccaatctgc tcggagtgct ggctactttg
     3661 ataacgagtg gcatcaactt gagggaggct gtggaggcgc tgagagcgat acggccggta
     3721 gcgggaagaa tggaacaaat gggaggggga gattcgcctc ttatcgtgat cgattacgcg
     3781 catactccgg acgccctcga aaaagtgctc atgacggtaa gagaaa
//
LOCUS       DASSHJ010000197         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_666500, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000197 DASSHJ010000000
VERSION     DASSHJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666500"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFI43_07660"
     CDS             complement(<1..299)
                     /locus_tag="VFI43_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7062039.1"
                     /translation="MELGMIGMGRMGGPMSQRLLNAGHQVVGFTRSLGGLAQLEKNGG
                     VAASSLEDLVARLKAPRAVWLMIPAGAPVDDAIERLQPMLQPDDVIIDGGNSNYK"
     gene            complement(354..1490)
                     /gene="tal"
                     /locus_tag="VFI43_07665"
     CDS             complement(354..1490)
                     /gene="tal"
                     /locus_tag="VFI43_07665"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7062040.1"
                     /translation="MNPLKQLLQHGQSVWLDSISRNLITSGELQRLITEDGLRGLTSN
                     PTIFEHAIEHGKEYDDALRELLRNNEKQSDKALFEAIAVEDIRMAADVLRPVYDDTGG
                     ADGYASLEVSPQLARDTQGSILEARRLWRAVQRPNLMIKIPATPEGIPAIEQLTSEGI
                     NVNVTLMFSLSHYDAVSRAYITGLGRWSASSPGGVKEAWPYSVASFFVSRVDTMVDPI
                     LEKIGAPEGLALRGKIAIANAKLAYQRFRETFYGEPFAALRRKGARVQRPLWGSTSTK
                     NPNYSDVLYVEELIGPDTVDTIPPKTLAAFRDHGRVRDTLEEGIEQARREIAELGNLG
                     IDLNAITEQLQKDGVDAFAASYEKLLNTLKKKRQELLATANHAV"
     gene            complement(1842..2633)
                     /gene="gloB"
                     /locus_tag="VFI43_07670"
     CDS             complement(1842..2633)
                     /gene="gloB"
                     /locus_tag="VFI43_07670"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HET7062041.1"
                     /translation="MTTLNVVAVRAFIDNYIWVIRDHLHAVVVDPGDASPILDYLRHE
                     KLKLVAILNTHHHNDHVGGNAALLREFSVPVYGPATENIPTVTHRLKECPDKENEEGC
                     AYIPEFSLSLHVLDIPGHTAGHIAYYGANLLFCGDTLFACGCGKLFEGTAQQMAYSLQ
                     KLAELPNETLVYCGHEYTLNNIRFARVVEPDNQALSKREADVEQLRKQDLPTLPSTIA
                     MEKATNPFLRCQQPEVIKSASNHGGKQLSDPVSVFATLRDWKNHF"
     gene            2762..>3534
                     /locus_tag="VFI43_07675"
     CDS             2762..>3534
                     /locus_tag="VFI43_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7062042.1"
                     /translation="MPTRNAVEWFNTCLGQYLLEREQNYYDQAVANVFGYHAMQIGFS
                     QYPFLRANRMPLQFCVGAEAGSAVRGAPDFLPIETNSIDLVLLPHILEFSANPHQILR
                     EVQRVLMPEGHVIIGGFNPRSLWGLRGLFGSIEDNFPWRGNFIARPRLKDWLALLDFE
                     ITEDRLCCYTPPFSKEKWLKRFHFMEAAGDRWWRFSGGVYFLTAVKRVHGMRVIKPEW
                     KDVRAAKRRVAAISQNARPQKVTGDTSGSRPARAARTRAR"
BASE COUNT          767 a          942 c          970 g          855 t
ORIGIN      
        1 tccttgtaat tcgagttgcc gccatcaatg atcacgtcgt caggctggag cattggttgc
       61 agtcgctcga tcgcatcatc caccggagca cctgcgggaa tcatcagcca tactgcgcga
      121 ggcgccttca atcgcgcaac cagatcttcc aacgatgatg cggcaacgcc tccatttttc
      181 tcgagttgcg cgagtccacc caggcttcgg gtaaagccaa cgacttgatg ccctgcattc
      241 agcaatcgct gtgacattgg accgcccatg cggcccatgc cgatcattcc tagttccatt
      301 cgattttctg cggcttattt gattgaaggg gaaaaggatt gtctacgagc gattcagacc
      361 gcgtgattgg cagttgcaag aagctcctgc cgctttttct tcagagtatt cagcagcttc
      421 tcgtacgaag cggcaaaagc atctacaccg tccttctgta gttgctcggt gattgcgttg
      481 aggtcgattc ccaggtttcc caactccgca atttccctcc tcgcctgctc gataccttcc
      541 tcaagcgtat cgcgcacgcg gccgtgatca cgaaacgcag ccagggtttt tggcgggatc
      601 gtatcgacgg tatctggtcc gatcaactct tccacgtaca gcacgtccga gtaattgggg
      661 tttttcgtgc tcgtgctgcc ccagagcgga cgttgcaccc gggcaccctt gcgccgcaac
      721 gcggcaaacg gctcaccgta gaatgtttcc ctgaaacgct ggtaggcgag cttggcgttc
      781 gcgatggcga tttttccgcg caaggcaagt ccctcggggg cgccaatttt ctcaagtatg
      841 ggatcgacca tggtatccac gcggcttaca aagaaagatg caactgaata gggccatgct
      901 tctttcaccc cgcccggcga agaagccgac cagcgaccaa gaccagtaat atatgcgcgg
      961 gacacagcgt cgtaatgact cagcgagaac attaaggtga cgttcacgtt gatgccctca
     1021 ctggttaatt gttctattgc gggaatgcct tccggcgtgg cgggaatctt tatcatcagg
     1081 ttgggccgct gcactgcccg ccagagccgc ctcgcttcca agatgcttcc ttgcgtatcc
     1141 ctcgcgagtt ggggagaaac ttcaaggctg gcataaccat ccgcccctcc cgtgtcgtca
     1201 taaaccgggc gaagcacgtc agcggccatg cgtatatcct cgacggcaat tgcttcgaac
     1261 agtgccttat ctgactgctt ctcattgttt cgcagaagct ctctcaacgc atcgtcatac
     1321 tctttgccgt gctcgatcgc atgctcgaaa atagtcggat tgcttgtcaa tccacgcaaa
     1381 ccatcctcgg ttatcagacg ttgtagctcg ccgcttgtga tgagattgcg gctgatcgaa
     1441 tccagccata cggactgacc gtgttgcagt aactgtttca agggattcat gcctgttctc
     1501 ctacgcggtt tcggccggga ggctatgcac ccgtcctggg ctatggggta tgtgctgaca
     1561 tatgtaaagt aaaacaggat tctgtatatc ttagggttta tcgaagtaaa gcacaattct
     1621 gtgacaatgc aacctcccga ttattcatgc ccgacaaaga aaatttaaaa caggcggata
     1681 tgcctaatcc tcgagagcat gggaagggtt ttgagtttga agaaacagct gtggctgcaa
     1741 tcagggagga agcaattctc gcttcacagt tggatctgct tccgctttcc gggaagcaga
     1801 tggaagatgc ttttgacttg gaaaaggggc ccttgcaaat tctagaaatg atttttccag
     1861 tccctgagcg tggcgaatac gctcaccgga tcggataact gtttcccccc atggttgctt
     1921 gcgctcttga tgacttccgg ttgctggcag cggagaaacg ggttggttgc tttttccatc
     1981 gcgatcgttg acggcagtgt aggcaagtcc tgcttccgca attgttccac atcagcttcc
     2041 cgtttgctta acgcctgatt atccggttcc actacccttg caaagcggat gttgttcagt
     2101 gtgtattcat gcccgcaata aaccagggtt tcgttgggca gttccgccag cttctgcagc
     2161 gaataagcca tttgctgcgc cgtaccttcg aacagcttgc cacacccaca ggcaaacagg
     2221 gtatcgccac aaaacagcag gtttgcgcca taatacgcaa tatgtccggc agtgtgacca
     2281 ggaatatcca gcacgtgaag gctgagcgag aattccggaa tatacgcgca gccttcttca
     2341 ttttctttgt ccgggcattc tttgaggcgg tgagtgaccg ttggaatatt ctctgtcgcc
     2401 ggaccgtaga cgggtaccga aaactcccga agcagcgcgg catttccgcc cacgtgatca
     2461 ttatggtgat gggtgttgag gatcgcgaca agtttcagct tctcgtggcg caggtaatcg
     2521 agtatggggg aagcatcgcc agggtcgacc acgactgcgt gaaggtgatc ccggataacc
     2581 cagatgtagt tatcgatgaa tgctcgaacg gcaacgacat taagtgtggt catggtagcg
     2641 gtgtggtcga gtcaggatgg cttgttcaat aatacagcaa gggaacggat aatgagcgag
     2701 gcgcggatcg aaatcgattc actccgttca cgcagtccag ccggcgcagc tcacattata
     2761 tatgccgaca cgaaacgcgg ttgagtggtt caatacctgc cttgggcagt atctgctgga
     2821 gcgggagcag aattattacg atcaggctgt tgcgaatgtt ttcggctatc acgcaatgca
     2881 gatcggcttt tcacagtatc ctttcctgcg cgccaaccgt atgcctctgc aattttgtgt
     2941 aggggctgag gccggctctg cggtccgggg agcacccgat tttctcccga tcgaaacgaa
     3001 cagtatcgat ctcgtgcttc tgccccatat ccttgaattc agtgccaatc cccaccagat
     3061 cctgcgggag gtgcagcggg tcttgatgcc cgagggccac gtcattatcg gcggattcaa
     3121 tccccgcagc ctttggggtt tgcggggtct gttcggttcg atagaagaca attttccgtg
     3181 gcgaggcaat ttcatcgccc gacccaggct caaggactgg ctggccctgc tggattttga
     3241 gatcaccgag gaccggttgt gctgctacac gcctccattc agcaaggaaa agtggctgaa
     3301 gcgttttcat ttcatggagg cggccggcga tcggtggtgg cgattttccg gaggggtata
     3361 tttcctcaca gcggtcaagc gggttcacgg aatgcgggtt atcaagccgg agtggaaaga
     3421 cgtaagggca gccaaacgac gtgtggcagc gatttcgcag aatgcaaggc cacagaaagt
     3481 gaccggtgac acaagcggga gcaggccggc aagggcggcg cgaacgcgcg cgcg
//
LOCUS       DASSHJ010000198        15975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_993356, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000198 DASSHJ010000000
VERSION     DASSHJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993356"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1637)
                     /locus_tag="VFI43_07680"
     CDS             complement(357..1637)
                     /locus_tag="VFI43_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc chelation protein SecC"
                     /protein_id="HET7062043.1"
                     /translation="MADDRTHAVPRAMHWLNSMHGRAVREALDSRFFGVLEDDEYDQL
                     PDQYNDAYQQIMTNALEWLLADGVITVKGKEQRVADAVLGRGGPLFSAEQRRWIELLA
                     AAPLRLYEVVDVVAGECMTLKDILLPDRPPVLVQEKSGSQRATRFDVIGARLIPFKDQ
                     FELSGAAYLFPRNRIWDLLKDLRDELDGLEPDSCPAKEVTGVLIPHHWLMLWVTRFEV
                     PQVVDHATGEPVLFITDHYRVQEWEALDRALAAQTDVDGSRETGWNRLLEGKDGFVRA
                     RLSIDLSKYSDRIKVSYRTQTHADEGRPWFEAIAGSTVTFISREISDSKGVFGGSPPA
                     DEEEPVPPSLPPEIATQLMEKSIRQYYANWADEPIPVLNNKTPREAVQTAEGLEQVKF
                     LLRTYEQGEAQQAKAQSREPVSYDFLWQSVGIAP"
     gene            2352..2861
                     /locus_tag="VFI43_07685"
     CDS             2352..2861
                     /locus_tag="VFI43_07685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7062044.1"
                     /translation="MTFAIRRALESDAASLIDLRRWLFEETSFMLWEPAEFKQTEDDE
                     RARIIRLNRQPNSVILLAEAGMQLVGVLSATGDDRKQLRQTALLSIGVVKSHWSQGIG
                     SRMIQDAINWSLAAGIKRLELTVHTSNLRAISVYMRYGFEVEGLRRSSLLVNGRYINE
                     YLMALIHEG"
     gene            3277..3747
                     /locus_tag="VFI43_07690"
     CDS             3277..3747
                     /locus_tag="VFI43_07690"
                     /inference="COORDINATES: protein motif:HMM:NF028515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062045.1"
                     /translation="MQKIDVDDDVYQFLESKVQGFGDTPNTVLRRLLGIGASKLQASN
                     SGDIKHPHATSMRGKAPKANLRDLVRVGSLSEGQVLYLHDYRGTKVGGVEACIHGNNL
                     EYKGQIQSMSALARAHMKIQGYDNDSYRGPEHWFTAQGQSVKDLWIEYLKAFPR"
     gene            3914..4294
                     /locus_tag="VFI43_07695"
     CDS             3914..4294
                     /locus_tag="VFI43_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7062046.1"
                     /translation="MQTNPVNWFEIYVEDMERAKAFYEAVLDGKLEHLPSPESEPCEL
                     WAFPMSHEGYGSAGALIKMEGCAPGDGGTLIYFKCDDCAVEAARAVENGGRIFKEKMS
                     IDKYGFIALVYDTEGNMIGLHSMK"
     gene            4759..5106
                     /locus_tag="VFI43_07700"
     CDS             4759..5106
                     /locus_tag="VFI43_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062047.1"
                     /translation="MYEQQIVRPRSITLVLVIALSVAVSGCVISGHEIVQNRVVIPPG
                     AVAKVRAVCPSGKKVTGGGFDVEIPQDVRVYASDPGDSQGNIVSDGWRVMVRNEGTTA
                     RQATAIAVCASAH"
     gene            5725..6774
                     /locus_tag="VFI43_07705"
     CDS             5725..6774
                     /locus_tag="VFI43_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HET7062048.1"
                     /translation="MSLLSISHLIHRHLLWFLISAYVLAMIFPLPGLWIRSMSFGEVN
                     LFQQKTHVSLLMVMLAILMFNAGLGLKLSDLKSLVDCKHLLLTGLAANLIIPITYIWA
                     MTIPMQLWYEPFEVQYILVGLALVAAMPIAGSSTAWAQNCNGNLALSLGLVFFSTILS
                     PIVTPVTLNILGEMASEEYEKVLHGLAAYGSGAFLGLWVVLPSLIGIGTRLLIGRDRD
                     GSGSRHIKLANSIVLLLLNYSNASVSLPQAVAERDYDFLAITLAITAGLCAALFAAGY
                     GLSRFFKLNRPEEISLMFGLGMNNNGTGLVLASLALSAYPNIMVPIIFYNLVQHIVAG
                     TVYEVVSKRQGNPDT"
     gene            complement(7008..7940)
                     /locus_tag="VFI43_07710"
     CDS             complement(7008..7940)
                     /locus_tag="VFI43_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062049.1"
                     /translation="MVRVHQIPVVGPFRLDLTVNALRRLPTNIVDLLTPEGGYLRALS
                     GPPQPLIVRVSQTPHNSSLSIMLDGDCRDESQTLELLQKMLGTGKDISDFDKAAVHIP
                     WLALLVERMRGVKPPCYPTLWEACVNAIVFQQLSIHAAGAIIRRLIVASGQRVETDWA
                     PIPLYAFPRAEAFKEAADDTLRATGLSASKVATLRRVAERLLSGALEAKKLEEATSQD
                     AIVDLCRIKGIGRWTASIILLRGMGRLDVFPQNDSGVLRNIMLLAGTADSGSPEILDS
                     LGPQRGMLYFYLLLARLEARGQIDRPSFPSQAGS"
     gene            8203..8418
                     /locus_tag="VFI43_07715"
     CDS             8203..8418
                     /locus_tag="VFI43_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HET7062050.1"
                     /translation="MSQRFKVGDHVTWNSEAGHVSGKIIKVHTENVVYKGYTHHASDE
                     EPQYEIKSDKTDHIAMHKGTALKKIND"
     gene            8627..8815
                     /locus_tag="VFI43_07720"
     CDS             8627..8815
                     /locus_tag="VFI43_07720"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HET7062051.1"
                     /translation="MPENTARSDSQGTNAPAQLMQMLFGFMTSQAISVAAELGLADLL
                     KNGPKSPDELAQATGARA"
     gene            8831..9667
                     /locus_tag="VFI43_07725"
     CDS             8831..9667
                     /locus_tag="VFI43_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7062052.1"
                     /translation="MRALASVGIFAEVDGGRFQMTPLAEPLRSDAPNSLRDFSIFLGA
                     DWHWQAWGDLAGGARTGEPAFEQVHGKAFFEYLGENPGPAQIFNDAMTSLSTAASAAV
                     VNGYDFTGINTLVDVGGGHGLLLASILKENPRMTGVLFDAPSVVAGASGVIEAHGVSG
                     RCETLGGDFFVSVPAGGDAYIIKHIIHNWDDDRASTILQNCRQGMSPSGKLLVVEMVI
                     PESNAPSPGKLLDLEMLVLFLHAHERTEGEYRALFERSGFRLSRIVPTPSPYSVIEGV
                     PM"
     gene            complement(9809..10063)
                     /locus_tag="VFI43_07730"
     CDS             complement(9809..10063)
                     /locus_tag="VFI43_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7062053.1"
                     /translation="MNASAKQKLREFLKEALENHGDRSEFGDGESLFASGRLDSFTMM
                     NLVMYLEQAFALDFSDLEFDAGLVDSVNEIEGLVDSKLTA"
     gene            complement(10063..11559)
                     /locus_tag="VFI43_07735"
     CDS             complement(10063..11559)
                     /locus_tag="VFI43_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062054.1"
                     /translation="MTAAISPSAESGAPAAGGKVRESRLRRFLAIDTRESFITLAQTA
                     AGQLLITFTAILAVAPYFGIWAGALAVAAAMTAVSQPALRDRILFAATWATAFVGTVL
                     GENDTLDNIVAVLQQQMVDLSPAVLAIAFLLLLLVAATGALYWVRRNPQSILARRPLV
                     TLLALEALLCGLASVEFMQGLPSVMLWAAIFVLTPYIWFLPYAIVDVRSRGGSSSLMQ
                     LAVLRPFWSPAYLPFGKGAAFLRKHLAKTPRDLAITQLKGVKLLIWATVLFALRDGLS
                     WGFEAQLQIPALAQALDAFLRGQPYPLIVGWGAMILSTAKFSLKVAAWAHLFIGVARL
                     AGYRLPRGSWRPLESRTLMDYFNRFHYYFKEMLVDFFFIPTFFKVFRNHPRLRMFFAT
                     FMAAGVGNAIWHFTRDIDLVATLGPTAALETYTSYAFYCVVLAIGVGISQVRITMGIR
                     PSPTVIGRLYSFLFIWSFVVCMHVFSDESRNHTFEERFSFLVSLFGVS"
     gene            complement(11556..13130)
                     /locus_tag="VFI43_07740"
     CDS             complement(11556..13130)
                     /locus_tag="VFI43_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET7062055.1"
                     /translation="MPKNYNLACAISHHGREAPQAPAVVCNGQSLTYAELAERAAVLA
                     ARITGSPGWQRNKGQPQIGILASRGIDACVALLGACWAGATYVPISLKQPEERILSLL
                     GQCRLSAIIADDIGMKLLTERLVAVCPQPIIHTSFAATSDRTTGSVQSVANPRTILEE
                     PVLMTPSDTAYIIFTSGTTGAPKGVMISAGAVRHYVTTIVEQLGLRATDRALETCEIS
                     FDFSVHNMFSTWEAGASLHILPANKVMNAVKFVRNSALTVWNSVPSLAGMLRQVKALA
                     PGSLACLRITVFGGEQLPASTVTAWQNAAPSSVIVNLYGPTEATVFCLAQVVAIPLPL
                     TPERDVIAIGTPLPGNDAAVIDKDGQTLPDGSTGELVIAGKQLADGYLEAPELNAARF
                     PTLNGKRWYRTGDRAIRDAAGRFHCLGRIDNQVKVLGYRVELEEIDAHLRVVSGADVV
                     GSVAWPLADGMASGIVSFVDTSEIAAERIIDLLKTRVPPYMLPNRIIFLKKMPLNPNG
                     KVDRSTLRQLLENDPA"
     gene            complement(13248..13613)
                     /locus_tag="VFI43_07745"
     CDS             complement(13248..13613)
                     /locus_tag="VFI43_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062056.1"
                     /translation="MKTRTALVILATMAVLASCAHIDPHPMDMTSAVQNAKTSADHEA
                     LARHYDDAAKSMQAKADQQKRMLAEYHRHAYYYGRRTDDLIEHSEALVRVYEEAARAN
                     ENLAHYHRLLAEDTSEMES"
     gene            complement(13786..14076)
                     /locus_tag="VFI43_07750"
     CDS             complement(13786..14076)
                     /locus_tag="VFI43_07750"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HET7062057.1"
                     /translation="MKFIQTLILTLALATPGLGRGEDATTHRTTAVVKSVDTANGKVK
                     LAHEPVRSLNWPAMTMNFSVKEPALFDKLTVGKKVEVEFVQQGSNSVVTGVK"
     gene            complement(14233..14985)
                     /locus_tag="VFI43_07755"
     CDS             complement(14233..14985)
                     /locus_tag="VFI43_07755"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET7062058.1"
                     /translation="MHKAQSHILLSLLVIIASAWGYLIYQHWQMTALPMSEMWMPPSE
                     ASEWKSIDFVLVYLMWAAMMAAMMLPSAIPMILAFLQVAKRHYPAPRNITFLFASSYL
                     TVWLLFSVGMTLLQWQLHGLQMISPMMETESELVSAVIFLGAGMYQLTPVKNRFLRFC
                     QSPAGFLLNEWREGAKGAFHMGLKHGGACVGCCWAQMLITFAVGVMNLVGMALITLLI
                     LVEKVVPSKSLFICRASGTILMGWGLLRLLMA"
     gene            complement(15119..15724)
                     /locus_tag="VFI43_07760"
     CDS             complement(15119..15724)
                     /locus_tag="VFI43_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET7062059.1"
                     /translation="MDNADWSLSGGYFETCNCQTSCPCIWLQPPSEGDCKLLVAWHID
                     SGHSDGQGLENLNVALVCYSPGNMKEGGWQAALYVDERANDAQFNAITQIFSGKQGGH
                     FAILMTFVSEVLGVKKVRMDYTEEGNRRQLAIPGITQADIESIKGITGGEATINNPPL
                     CVVTSHPAVVARSRHYEYHDYDKNWLFSNRNGFYSPFTYHP"
BASE COUNT         3696 a         4353 c         4305 g         3621 t
ORIGIN      
        1 gcctctcatt ggaatgcttc tgttgacctt cctgggatac aggatcatgc aatccaagcg
       61 ggtgcaggca tggaggaata tatcgcgccc ccgccgatag cctttcgagg gaattccata
      121 taagtccgca ccggtgtggg agacttcacc ggatacggat aacatatcgg cccgtgtatg
      181 ataacgatgc aatccaggcg ccgcttttgc tcacggaccc cttccgggtc tgggcggtgg
      241 tactgacgaa tcatccttct tgggcaccat ataccccctg ctgtcgttct cgtcgtccac
      301 cctgtgattg cctcgacttt atagcagcac tcgcaggaag acgcgttcag cttcccttac
      361 ggcgctattc cgactgactg ccagagaaag tcataggaaa ccggttcgcg ggattgagct
      421 tttgcttgct gagcctcgcc ctgttcgtag gtacgtaaca agaattttac ctgctcgagc
      481 ccttcagccg tttggaccgc ttcgcgtggt gttttattgt tcaatactgg tatcggctcg
      541 tcagcccaat tcgcataata ttgccggata ctcttctcca tcagctgtgt tgcgatttcg
      601 gggggcaagg agggtggcac gggttcctcc tcatcagcag gcggggaacc cccgaaaacg
      661 cctttggaat cggatatctc gcgactgata aaagtaacgg tggagccggc aattgcttcg
      721 aaccacggcc gcccttcgtc ggcatgcgtc tgtgtgcggt aagatacttt gatccggtct
      781 gaatatttgc ttaaatcgat gcttaggcga gcgcgtacaa aaccgtcctt tccttccagc
      841 agcctgttcc agcccgtttc gcgggaacca tcaacatcgg tctgcgccgc caaggcccga
      901 tcaagcgctt cccattcctg aaccctgtag tgatcggtaa tgaacaggac aggttcgccc
      961 gttgcatgat cgactacttg aggcacttca aatctggtga cccacagcat gagccagtga
     1021 tggggtatga ggactccggt cacctccttg gcgggacatg aatccggctc gaggccatcc
     1081 agttcgtctc taaggtcttt cagtaaatcc cagattcgat tgcggggaaa gagatacgca
     1141 gcgccggata gctcgaattg atctttaaaa gggatcaatc gagccccaat aacatcgaat
     1201 cgagtcgctc gctgcgatcc ggatttttcc tggacgagga ctggcggacg gtcgggcagc
     1261 aaaatgtcct tcagtgtcat gcattcccct gccactacat caacgacttc gtaaagcctg
     1321 aggggcgccg ctgccagaag ctctatccat cgccgctgtt ccgcagagaa taagggacca
     1381 ccgcggccga gcaccgcatc tgcaacacgc tgttctttac ctttgactgt tattacaccg
     1441 tccgccagca gccattccag ggcattcgtc atgatctgct gatacgcatc gttgtattga
     1501 tccggcagct gatcgtattc gtcgtcctcc aggaccccaa aaaaccggga atcgagcgct
     1561 tcgcggacag cccggccatg catcgagttc agccagtgca tggcacgcgg gactgcatga
     1621 gtccggtcat ccgccatctt gctttcatca accctgagac agcactgctt atactttttt
     1681 ccgctgccac aggggcacgg atcattacgt cccaatcttt tcatctcaat ttaatcgctg
     1741 atttcaagag gcttaagtgc ttgtaaacgg aaagatgccg gggtcttgaa ggggtaaaat
     1801 atagcatatg cacctgacag tttttctgcg agcattaatc ctttggacga acaatggatt
     1861 gacctgcgat gacggacgct ggttttaccc cagtgttctt gctgtttatc gcagttgtac
     1921 ttcagtccaa tagtaaagcg ggggtatccc ggtaagatgt cgttgcgggg cgatttttca
     1981 accctgcaca agtgtaacgt cccgccccgg ctcccaggta atgctgcgag ccgggtttgt
     2041 ttttcaggtt ggaactccgc cagaaacaac gcagggctgc ttctcattgg ccccttagta
     2101 gtcccttagg cgtagttttc ctgacaatgc ctaatgaaac tgctcttatt taacgttggg
     2161 ataaaagccg ggagcgaaca ttcttcaatc atgccattcg cagtggtatt cggatgattg
     2221 agaattgaag gaacgaatcg cgcgtcaata atatgcagta cctataaata tgcagaacct
     2281 atactcagca cgtcgaaaaa tctctggacg aagcatccga tatcactgtt aacgctggga
     2341 tcacaacctt catgacattc gccatccgcc gtgctcttga atccgacgca gcttccctga
     2401 ttgatttgcg cagatggctg tttgaagaaa cgtcgttcat gctttgggaa cctgctgagt
     2461 tcaagcaaac cgaagacgac gaacgtgcac ggattatccg cctgaatcga cagccgaata
     2521 gcgttatctt gcttgcagag gcggggatgc aactcgtggg cgtactgtcg gccacgggcg
     2581 atgaccgcaa gcagctgcgc caaaccgcgc tgctatcgat aggcgtcgtc aaatcgcatt
     2641 ggtcccaagg catcggctcc cgaatgatac aggacgcaat taactggtct ctggcagccg
     2701 gcatcaagcg gctcgaactg accgttcata cttcgaatct tcgcgccatc tccgtgtata
     2761 tgcgctacgg gtttgaggtc gaaggcttgc gccgctcgtc gctcctggtg aatggcaggt
     2821 acatcaatga atacctgatg gccctcatcc acgagggcta atcattcgat caactcgaaa
     2881 acccgcccct agttggtttt cctgtaccac ttgccgaccc acccgcagca aatcacccaa
     2941 atcactgtta cctagttctg gcatatccct ccgcgctcga atacgctgat gttttgctgc
     3001 gtggtgcgtc atgttcgata gtgcatcgtg gtaaaaagta tacggggccc ctgttccgcc
     3061 cctgaccgcc tcctaaaata ccccgtgcga atacagctct aactcctcat tccagtaagc
     3121 tcatctctcg ccgcgagcgc gaaaaaagcc catattacgg agcacttcat gcctcccctt
     3181 atccttatcc ttgatcttat catttcaatt gctagcaagg ataagaattt ataagtatat
     3241 tcttatcctt gctttctttg ataaggaaat ccggttatgc aaaaaatcga tgtcgatgac
     3301 gacgtctacc aatttctcga aagcaaagtt caggggtttg gcgatacacc aaataccgtt
     3361 ctgaggcggc tccttggtat tggcgcgtct aaacttcaag cgtcgaacag tggtgacatc
     3421 aagcaccccc atgccacgtc gatgagaggt aaggctccaa aggccaatct tcgggatctg
     3481 gttcgcgtag gcagcctttc agaagggcag gttctctacc ttcacgacta ccgcggcacc
     3541 aaggtcggcg gtgttgaagc ctgtatccat ggcaataatc tagagtacaa ggggcaaata
     3601 caatcgatgt ccgcccttgc ccgcgcgcat atgaaaattc agggctatga taacgactca
     3661 taccgaggcc ctgagcattg gttcacggca caggggcaaa gtgtcaaaga tctttggatt
     3721 gaatacttga aggcatttcc aaggtaatgt tctacgagta aacgggaatg ctgggcgaaa
     3781 aagccttctc ctcgcaggga ttcgatcact tcatttggca aagatggtac gatttgcgtg
     3841 aaacaaaatc gcatcggctg ccgccagaac gccatgcact tcaaataata cccccgagat
     3901 aggagcaaac gtcatgcaaa ccaacccggt gaactggttt gaaatttacg tggaagacat
     3961 ggaacgcgcc aaggcgtttt atgaggctgt gctggacggg aagctcgagc atttgccgag
     4021 ccccgagtcc gagccgtgcg agttgtgggc ctttcccatg tcccatgaag gatatgggtc
     4081 ggcaggtgcg ctgataaaaa tggagggttg cgctccgggt gacggcggta ccctgattta
     4141 cttcaaatgt gacgactgcg cggtagaggc ggcccgtgcg gttgagaatg gcggacggat
     4201 tttcaaggag aaaatgtcga ttgacaaata tggcttcatc gccctcgtgt acgacacgga
     4261 ggggaatatg atcgggttgc attcgatgaa gtaatgctct tccgcaagcg gtccgccagt
     4321 tgcctttgct ccggagaggc gaaggacgag gtatggagcg cccccttcct gtgaacacca
     4381 gccgataaat cgttttgcca gagagaccgg cctcactaca ctttatcctg accatggatt
     4441 ctcttcgacg gggagccgct caagggtcac atgcttcgct gtagggcatt atcccggtcg
     4501 tggtccctca cgcgacgctt tacgctttag tgaccccctc tcctgccctc ttcggtaaat
     4561 gggagagagg aattgatgag gttcatgcac tacaaggcga cgcttgagtt actcttgagc
     4621 ctaagctccc gggctgaatc tgttacttga ctggaggttt cactgaacta gtattaatag
     4681 gggtgaggca atgagcgtgc agaggaatgt ctacccgcat ccctgtcgca tgaaatcaac
     4741 gcatgacaaa gaggccttat gtacgaacag cagatagtac gtccacgatc cattactttg
     4801 gtgcttgtca tcgctctcag tgtcgcagtc tccgggtgcg tgatctccgg ccatgaaatc
     4861 gttcagaacc gtgtcgtgat tcccccaggg gcggttgcca aggtaagggc cgtatgccca
     4921 agcggcaaga aggtgactgg aggcgggttc gacgtcgaga tacctcagga tgtgagggta
     4981 tacgcctccg atccgggcga cagccaagga aatattgtca gtgatggctg gcgcgtgatg
     5041 gtgcggaatg aagggacgac cgcgcggcag gcaacggcca tcgctgtgtg tgcatcagcg
     5101 cactagtctg ctcaagtggt gcgaggttca cggttctctc gggtgaactg cgaatgatgt
     5161 tctttatcct cgcgttgcaa tcgctaccca ggtatcgaac aggtagttga acgtttattt
     5221 ttctccttga agtccacaaa acagtcgtca tggacactgt cgtcaccttc agggggagcg
     5281 ctcattcgga ctttgtaacg aaaaggatat ttgacacgcc taatccaggc ttgggcgtct
     5341 gatcgccctt tatgctcagc ctgccctgcc cccaaacccg gggatttccg cctggaataa
     5401 tcgtccccga ccgccggctg ttccgtgctc tccttttggc atcctccgtt ttttgggccc
     5461 cctccccttt cctcccaaag accccgacta attctccagc gtctgcacct cgcgccctac
     5521 cctccttccg ccgagttatg tcgatcgaat gtcctgcaac gtacgtccca tcgcctattt
     5581 ttaacttatg attgatcgaa aaatggaaaa tttaccttct ttgcatcgac agcgacttcc
     5641 ggtctctttc gaacggcgat agcaccgtca acgaagacca atagagcctc ccggccgcgc
     5701 gaagcaggtg gaaggataag ataagtgagc ctgctctcga tctcccatct gatccatagg
     5761 catctgctct ggtttttgat cagcgcgtat gttctcgcca tgatttttcc gcttcccggc
     5821 ttatggatca gaagcatgtc tttcggcgag gtgaatctct tccagcagaa aacacacgtg
     5881 tcgctgttga tggtcatgct cgcgatactc atgtttaacg ccggcctggg gctgaaatta
     5941 tccgacctca aaagcctcgt ggactgcaaa cacctcttgc tgaccggact ggcggcaaac
     6001 ctgatcattc ccatcacgta catctgggcc atgacaatac ccatgcagct ctggtacgaa
     6061 cccttcgaag tccagtatat cctcgtgggg ctggcattag tcgccgccat gcccatcgcc
     6121 gggtcatcca ccgcctgggc gcaaaactgc aacggcaacc ttgcattgag cctgggactg
     6181 gtcttctttt ccacgatcct gagcccgatc gtcaccccgg taacgcttaa cattttgggc
     6241 gaaatggctt cggaggagta tgaaaaagtc ttgcacggcc tggccgccta tggctccggt
     6301 gcttttctcg gcctctgggt ggtattgccg tcgctgatag gcatcggcac acgcttgctg
     6361 ataggtcgag accgggatgg aagcggcagc cgccatataa agctcgcaaa tagcatcgtt
     6421 ttgctgctgc tgaattactc caatgcttcg gtgtccctgc cccaggccgt ggcagagcgt
     6481 gactacgatt ttctcgccat cacgcttgcc attaccgctg ggctttgcgc agccctgttt
     6541 gcagcaggct atggactgag ccgcttcttc aagctgaacc ggccggaaga aatttccctc
     6601 atgtttggcc tggggatgaa caacaatggc actggacttg ttctggcgtc actcgctttg
     6661 tcggcctatc cgaacatcat ggtaccgatc atattctata acctggttca gcacattgtg
     6721 gcgggcacag tgtacgaagt ggtcagtaaa aggcaaggca atccggatac ctgaaaatcg
     6781 ataaccggca atgaggcggg caagggtttt tgtctctcca tcaggagcag tgttggggca
     6841 agcaggatca cggggcgcgt tgaggggtgc tcctatgacg accgccgcgg cgagggtaac
     6901 tttctggagc gctttggcga cggcggcaac ggacggcgtc aagggttgtt tgcgtggcga
     6961 ccctggaatc tcgctgcctc ggtgcttgat gaccacggtt tgagtcctta ggacccagcc
     7021 tggctcggaa atgatggacg atcgatctgt cctcgggcct ccagacgggc aaggagcaag
     7081 taaaaataca acattcccct ctgtggaccg agcgaatcca ggatctccgg gctaccggaa
     7141 tccgcggtgc cggcaaggag catgatattc ctcaggactc cactgtcgtt ttgcggaaat
     7201 acatcgagcc ggcccatgcc gcgaagcagg atgatcgatg cggtccagcg gccaataccc
     7261 ttgattctgc agagatcgac aatcgcatcc tggctcgtgg cttcctcgag tttttttgct
     7321 tcgagggcgc ccgacagcag cctttcggca actcgcctta gcgttgcgac tttgcttgcg
     7381 ctcagcccgg ttgcacgcag cgtatcatca gctgcttcct taaatgcttc cgccctcgga
     7441 aacgcatata gcgggatggg cgcccaatcg gtttccaccc tctgtcccga agccacgatc
     7501 aggcgacgta tgattgcgcc cgccgcatgt atgctcaatt gctggaacac gatggcgtta
     7561 acgcaggctt cccaaagggt aggatagcaa ggcggcttta ctccgcgcat tctctcgacg
     7621 aggagagcga gccatgggat atggacagcg gctttgtcga aatccgagat atccttccct
     7681 gttcccagca tcttttgcag gagctccagc gtttgggact catcgcgaca atccccatca
     7741 agcatgatcg acagcgagct gttgtggggc gtctggctga cccgcacaat caggggctgg
     7801 ggtggtccgg atagggcgcg caaatagccg ccctcgggcg taagcaggtc gacgatattc
     7861 gtcggaagcc ggcgaagcgc atttaccgtc agatcgagac ggaatggccc aacgactgga
     7921 atctggtgta ctctaaccat cgtggcttcg gtgtggatcg agattcaccc atattcggtt
     7981 tgtccggggt ttctcattcg acgagcgcat ggctttgact tatggtgtga agcgatggaa
     8041 ggaacaagga gaattcctgt tatcggagcg aaattctcca actgttccct ccccccttct
     8101 cgaaaggcat aagaaacgcg ccggggcata tgggtagtac ctgtttgcac acatcatatt
     8161 attccgcatc aagggtggta aaaaagtgag gctatggaga gcatgtcgca acgattcaag
     8221 gtgggtgacc atgtaacgtg gaattcagag gccggccatg tcagtggaaa aatcatcaag
     8281 gtgcatacgg aaaatgtcgt ctacaaagga tatacccatc acgcgagcga cgaagagccc
     8341 caatacgaaa tcaagagcga caagactgac catattgcca tgcataaggg aactgccttg
     8401 aaaaaaataa acgactagaa acgcgatggc aaaagggccg taaaagtcga aattgatttg
     8461 atcccggcgc ctcctttttt acgattcaac ttcatttttt tattctgcac ctttttttcg
     8521 ctcctcatgg atggagcacc ctgtgcaacg tatttctcgc ggaacactgg taccgggaaa
     8581 caggaaagac ggaataatcg tgaacgtcaa tttttgagga aatccaatgc ccgaaaacac
     8641 agcacggagt gattcgcaag gcacgaacgc gccggcacag ttgatgcaga tgcttttcgg
     8701 tttcatgact tcgcaagcga tttcagtcgc agcggagctt ggtctggctg accttctcaa
     8761 gaatgggccg aaaagcccgg atgaactggc tcaggcaacc ggagcgcggg catagtcgct
     8821 ttatcgcatt atgcgtgcac tggcaagtgt gggtatcttc gcagaagtcg acggcggccg
     8881 cttccagatg acgccactgg ccgaaccttt acgcagtgat gcgccgaaca gcctgcgcga
     8941 cttctcgatt ttcctcgggg ccgactggca ctggcaggca tggggagatc ttgctggcgg
     9001 tgctcgaacc ggcgaacctg ccttcgagca ggtccatggc aaagcatttt ttgagtacct
     9061 gggcgagaac ccgggccctg ctcagatctt caatgacgcg atgaccagcc ttagtacagc
     9121 ggcaagcgcg gcagtggtca acggttacga cttcacgggg atcaatacgc tcgtcgatgt
     9181 cggcggcggc cacggtttgc tcctcgcctc gattctcaaa gaaaacccgc gaatgacggg
     9241 tgtgcttttc gatgcaccat ccgtcgtcgc gggagccagc ggtgtcatcg aagcgcatgg
     9301 ggttagcgga cgttgcgaga cgctcggcgg cgattttttt gtgtcggttc ctgctggtgg
     9361 tgatgcttat atcataaagc acatcatcca caattgggat gatgatcgcg cgtccactat
     9421 ccttcagaac tgccgccagg ggatgagccc gagtggcaaa ttgctggtgg tggagatggt
     9481 gatccctgaa agcaatgcgc cgtcacccgg caagctcctc gatcttgaaa tgctcgtgct
     9541 cttcctgcat gcgcatgagc gaacggaagg ggaatatcgc gccttattcg agcgatctgg
     9601 ctttcggtta agcaggatcg tgcctacgcc gtcaccctac agcgtgatag aaggcgtacc
     9661 gatgtgacgg ccacaggggt tgaaacgaag gaaacggccc agcaataagt gataagtgga
     9721 ccctgttccc tccaaagtca cttctcattt gatggctgct acggcgggct tcagcaatac
     9781 gcgaggatga tttcggcggc aacaccgctc acgcagtcag tttggaatcg accagcccct
     9841 cgatttcgtt cactgaatcg acgagacccg cgtcaaactc caaatcggaa aaatccaggg
     9901 cgaaagcttg ctcgagatac atgacaagat tcatcatggt gaaggaatcc agccggccgc
     9961 tggcaaaaag agactctcca tcgccaaact cgctgcgatc gccatggttt tccagggctt
    10021 ccttgagaaa ttcccgcagc ttctgtttcg cacttgcatt catcaggata ctccaaaaag
    10081 gcttacgagg aacgagaacc gttcctcgaa ggtgtggttg cgcgattcgt cgctaaagac
    10141 atgcatgcaa acaacaaagg accaaataaa gagaaaggaa taaagtctgc caattactgt
    10201 cggggaaggt cgtatgccca tggtgatacg aacttgcgag attccgacgc cgatcgccag
    10261 aacgacgcaa taaaaggcgt agctggtgta tgtttccagg gcggcggtag gacccagggt
    10321 ggcgacgaga tcgatatccc gagtgaaatg ccagattgca ttgcccacgc ccgcagccat
    10381 aaaagttgca aaaaacatcc gcaggcgcgg atggttgcga aacaccttga agaaggttgg
    10441 tatgaagaaa aagtcgacca gcatttcctt gaagtagtag tggaagcggt tgaagtaatc
    10501 catgagcgtc cttgactcca ggggacgcca ggagccccgt ggcagccggt aacctgccag
    10561 ccgggcgacg ccgatgaaga gatgggccca ggccgcaacc ttcagagaaa attttgcggt
    10621 tgacaggatc atggcacccc aaccgacgat cagcggatag ggctgtccac gcaggaacgc
    10681 atccagggct tgtgcaaggg ccggaatctg caactgcgcc tcgaatcccc agctcagtcc
    10741 gtctcggagt gcaaaaagca cggtcgccca gattagcagc ttgacccctt tgagctgtgt
    10801 tatagccaag tctcgcggcg tcttcgccag atgcttgcgg aggaatgcag cgcccttgcc
    10861 aaagggcaga taggcgggac tccagaacgg gcgcagcaca gccaattgca tcaatgacga
    10921 actgccgcct cgggaacgaa catccacaat ggcataaggc agaaaccaga tgtatggcgt
    10981 caggacaaaa atagctgccc acagcattac gcttggaaga ccttgcatga attcgacgct
    11041 cgcaagaccg cacaagagtg cttccagcgc taaaagagta acaagcgggc gtctcgcaag
    11101 aatggattgc gggttgcggc gaacccaata tagcgctccg gttgcagcga cgagaagcaa
    11161 cagcagaaac gcgatcgcca gtaccgccgg ggaaagatcg accatctgct gctggaggac
    11221 cgcgacgata ttgtccaggg tatcgttctc ccccagcact gtaccaacga aggcagttgc
    11281 ccaggtggct gcgaacagta tgcgatcgcg caacgcgggt tgcgacaccg cggtcattgc
    11341 agcagcgacg gcgagggcgc cggcccagat gccgaaatat ggggcgacgg ccagaatggc
    11401 cgtgaaggtt atgagcaact gcccggctgc cgtctgggcg agcgtaatga atgactcgcg
    11461 cgtatcgatg gcaagaaagc gccgcaaacg gctttcgcgc accttgccgc ccgcggctgg
    11521 cgctccgctt tctgcagagg gggagatcgc tgctgtcatg ccggatcatt ttcaagcagt
    11581 tgacggagcg tgctgcgatc caccttccca tttggattaa gcggcatttt tttcaaaaag
    11641 atgatgcggt tcgggagcat gtagggagga acccgggttt tcagcagatc gatgatgcgc
    11701 tcggcagcaa tttccgaagt gtctacaaag ctgacgatgc cgctcgccat accgtcagcc
    11761 agcggccagg cgacagaccc aacaacatcg gcacctgaga ctacgcgcag atgggcgtcg
    11821 atttcttcca gttcgacgcg atagcctagc accttgacct gattgtcgat ccgccccagg
    11881 caatgaaagc gacccgcagc gtcgcgtatc gcccgatcgc ccgtcctgta ccagcgttta
    11941 ccattcaggg tcgggaaacg tgccgcgttc aactcggggg cttcgagata accatcggca
    12001 agttgcttgc cggcaattac cagttcgccc gtcgagccat ccggcagagt ctgcccatcc
    12061 ttgtcgatga cagccgcatc gttgccgggt agcggcgtcc cgatcgcaat gacatcccgc
    12121 tccggagtga gcggcaacgg aatagcaact acctgggcca ggcaaaaaac cgtggcctcc
    12181 gtcggcccat agaggttgac gatgacgctg ctgggcgccg cattctgcca ggcagtaaca
    12241 gtactggcag gcaattgttc gccgccgaag accgtgatgc ggaggcatgc gaggcttccg
    12301 ggcgccagcg ctttaacctg acgcagcatg ccggcaagcg aaggcaccga gttccagaca
    12361 gtcaaagcgg agttccgcac aaatttcacg gcattcatta ccttgttggc gggcaggata
    12421 tgcaacgacg cgccagcctc ccacgtcgaa aacatattgt gtaccgagaa atcgaagctg
    12481 atctcgcagg tctccagcgc ccggtcggtg gcccgcagcc ccagttgctc cacaatcgtc
    12541 gtcacataat gacgcaccgc ccccgcagaa atcatgacgc cttttggggc gccagtcgtt
    12601 cccgaggtaa aaatgatata ggccgtatcg gatggcgtca tgagcacggg ttcctccagg
    12661 atcgtccgcg gattggcaac tgactggact gatccggtcg ttctatccga ggtggcagcg
    12721 aacgaggtat ggataatcgg ctgtggacag acagcaacga gacgttccgt gagcagcttc
    12781 atcccgatgt catcggcaat aatagcggac agcctgcatt gaccaagcag ggacaggatg
    12841 cgctcttccg gctgtttcaa gcttataggc acgtaagtcg ctccggccca gcaggctccc
    12901 agcaaggcaa cgcaggcatc aatcccgcgg gaagcgagaa tcccgatctg cggctggccc
    12961 ttattgcgct gccagccagg actgccggtg atccgggccg ctagaactgc agcacgctcg
    13021 gctagttcgg cgtaagtcag ggattgccca ttgcagacta ccgctggagc ttgaggagct
    13081 tcgcgaccgt gatggctgat ggcgcaagcc agattgtaat ttttaggcat atttctgttt
    13141 tacacgccgc acggcatgaa tagcgagcgc gtgggcagat gcgaaagtcg attgacaggc
    13201 gtcagaaaaa tccgaacagg agaaatctgc taaaaggcag caaaatatta agactccatc
    13261 tcacttgtat cctccgcaag gaggcggtgg taatgagcca gattctcgtt tgccctggca
    13321 gcctcctcat acacccgtac gagagcttct gagtgctcta tcagatcgtc ggtccgtctg
    13381 ccgtagtagt aagcgtgccg atgatattct gcaagcattc tcttttgctg atctgccttt
    13441 gcctgcatgc ttttcgccgc atcgtcatag tgcctggcca aggcctcatg atcggcgctg
    13501 gttttggcgt tttggacagc acttgtcatg tccatggggt gagggtcgat atgcgcacac
    13561 gaggctagca cagccatcgt agcaagaata acgagagcgg ttcgagtctt catcagcatc
    13621 tcccttttga attgttaata acattatagg cactagtgtt attacaatct gacgcgattg
    13681 aggccgaggg aaagaaatag ggtcgggctc gacgtaagta cgtgtaaccg gtttacccac
    13741 tctttatcaa gctgctgccg acgcagccaa cttcggattt ccgggttact tcacccccgt
    13801 aaccacagag tttgaccctt gctgcacaaa ctcgacttcc actttttttc caacagtgag
    13861 cttgtcgaac aatgcgggct ccttgactga aaaattcatg gtcatcgctg gccagttcag
    13921 acttcttacc ggctcatgcg caagcttgac ctttccgttg gctgtatcga ctgacttgac
    13981 taccgccgta gttctatgtg tggtcgcgtc ctcgcctctg ccaaggccag gggtggccag
    14041 agcaagagtc aggataagag tttgaatgaa tttcattaag ctggtccttt cgatgatggg
    14101 tcggtaaatg atctgatttt gatctgattt tgatcgactt gacggagttg cgccgggaac
    14161 gacccttcgc tccacctccc ctgctgagct ctaccgaact ctgacttatt aatcggggga
    14221 ggatggcagg tattatgcca tcagtaagcg caagagtccc cagcccatca atatcgtccc
    14281 gcttgcccgg caaataaaca ggctttttga cggcacaacc ttttcaacaa ggatcagcag
    14341 ggttatcaag gccatcccca ccaggttcat cacgcccacg gcaaacgtga tgagcatttg
    14401 cgcccagcag cagcccacgc aagcgccacc atgcttcaat cccatgtgga atgcgccttt
    14461 tgctccctcc cgccattcat tcaataaaaa accggcggga gactggcaaa atcgaaggaa
    14521 acgatttttg acaggggtca actggtacat ccctgcccca agaaaaatca cggcggagac
    14581 caattcactt tccgtttcca tcataggaga tatcatttgc agcccatgaa gctgccattg
    14641 caacagcgtc atcccgacgc tgaacagcaa ccagacggtc aggtagctcg aagcaaaaag
    14701 aaatgtgata ttccggggag ccggataatg ccttttggcg acctgcaaaa aagcgaggat
    14761 catgggtatg gcggatggga gcatcattgc agccatcatc gctgcccaca tgaggtatac
    14821 aaggacgaaa tcgatcgatt tccattcgga agcttccgag ggaggcatcc acatttccga
    14881 cattggcaac gcagtcatct gccagtgctg gtagatcagg tatccccaag cgctggcgat
    14941 aattaccagc aacgacagca gaatatggga ctgagcttta tgcatccttg ggcgggtgaa
    15001 agcgccccac cacctttaat gggggagatt tggcaaaaaa gactttggaa agccatttat
    15061 ccacgaaaac ggtgaattga ttcgatagcg cggcttgtcc gagtatgagc aaaaactgtc
    15121 aaggatgata ggtaaacggc gagtaaaaac cgttccggtt gctgaatagc cagtttttgt
    15181 catagtcgtg gtactcgtaa tgcctggacc gggcaaccac ggcggggtgg ctggtgacaa
    15241 cacataaagg cggattatta atcgtcgctt caccccccgt gatgcccttg atcgactcga
    15301 tatcggcctg agtgatccct gggatagcaa gttgccgcct gttgccctcc tccgtgtaat
    15361 ccatccgaac cttcttcact cccagcacct cactgacgaa ggtcatcagg atggcgaaat
    15421 ggcccccctg tttcccggaa aaaatttgcg ttattgcgtt aaattgcgcg tcgttcgcgc
    15481 gctcatccac gtaaagagcg gcctgccagc caccttcttt catgttccca ggcgaatagc
    15541 aaaccagggc gacgttcaga ttctccaagc cttggccgtc cgaatggccg gaatcgatgt
    15601 gccatgcgac caacagcttg cagtcacctt ccgacggtgg ttgcagccat atgcatgggc
    15661 aggatgtttg gcaattgcac gtttcgaagt acccgccgct caagctccag tccgcgttat
    15721 ccatcgtcgt tcccctattc aaaaaggatt tttcgttcac atatttccaa tatagagcaa
    15781 accaccgagg gaagttgaac atgaatcggc tggatgtgag acgtagagaa atctgtccga
    15841 gaaacttcga ggctaccgac tggcttgacg gcgagttcac cgcgcttagg gtgtcatttg
    15901 ccccctaacg gaatcgaccc cagaattaag gctacgcgct gatatcccat gcagccacgc
    15961 aacatcccta tttac
//
LOCUS       DASSHJ010000199        11387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_147134, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000199 DASSHJ010000000
VERSION     DASSHJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147134"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VFI43_07765"
     CDS             complement(<1..136)
                     /locus_tag="VFI43_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7062060.1"
                     /translation="MPLHIETPLIESPSISHKVGQSIWLKMESAQPSGSFKTRGIGYA
                     C"
     gene            682..1428
                     /locus_tag="VFI43_07770"
     CDS             682..1428
                     /locus_tag="VFI43_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062061.1"
                     /translation="MLETEKLDKLSCLLKEMTRPGISQPKAAPLLIMERQIAHSMIGF
                     LLLLGFAYFSQSEGQRLFGITPLELHDALLIVQWPLIACFVFFLVMLTRRALYIRRHR
                     KELVPFPFLYPPQKSALHRDAEFITQLLRFDKATLAYGLLQYSHRWSSPEGRAAVFVG
                     DLRKLGLVPTVVALFISVAMLFKGDSSPFLLFLQDLVVIVIIYYVAAFLGFLSRERPQ
                     QVIQLLEYAIQHADQCNATPHDAIASAPSD"
     gene            1668..1988
                     /locus_tag="VFI43_07775"
     CDS             1668..1988
                     /locus_tag="VFI43_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062062.1"
                     /translation="MKYYSPLKSKGMLPVLGAVLLTMAGVAHAQIPTPGQGDAMQRPA
                     DSMQPPVIPGKPFEKGTTRGEEAQAQNRFNKNEEEAMRHKLDTNKGYGSRQDGGSGSS
                     SGGY"
     gene            complement(2075..3223)
                     /locus_tag="VFI43_07780"
     CDS             complement(2075..3223)
                     /locus_tag="VFI43_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7062063.1"
                     /translation="MIKTKILIRLTVAVLILAVLGFAVWFATRPKPVEVELGTISRGV
                     VEATAVNTRAGTIKACRRAKLAPAAGGQIVKLWVKEGDRVGKGQPLLELWNSDIAAQR
                     DLAQRHLATAKERRREACIVADNARRQAERTHHLAAKGFLSPQASENADAEARARQAS
                     CDATASDVKRAQAEVKVTQAGLERTVLVAPFAGIVALITGELGEYTTPSPPGIPTPPA
                     VDLIDDTCLYVTAPMDEVDAPKLKVGQSARITLDALPGQSFPGKVRRIAPYVTEVEKQ
                     ARTVDVEINFEEIPKNALLVGYSADVEVVIQRRENVLRVPTQAIRQNDKVLVVNQESR
                     LEERTVETGLANWAFTEVISGLKEGDRILLSSDQDVKAGMQVRPKTSP"
     gene            complement(3275..4438)
                     /gene="dinB"
                     /locus_tag="VFI43_07785"
     CDS             complement(3275..4438)
                     /gene="dinB"
                     /locus_tag="VFI43_07785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7062064.1"
                     /translation="MIKVRVVSRRIAHLDMDAFYASVELLRYPELRGLPVVVGGRGDA
                     DPEALEGSGSRFLRLKSYAGRGVVTTATYEARVFGIFSGMGLMKAAQLAPDAILLPAD
                     FDAYRHYSRLFKAAVAAIAPRIEDHGIDEIYIDLSELGEDAATLARRLKQAVRESTAL
                     SCSIGVATNKLLAKICSDLDKPDGLVILGPDDMPEKIWPLPVKKISGVGPKANGKLSA
                     LGIVTIADLANADRSFLQAQFGRSYGNWLHEAANGIDDRPVITDSEPKSLSRETTFER
                     DLHSRADRHVLSEILTALCRELAGDLRRKGYVSRAIGIKLRYDDFHTITRNITLSSPT
                     ADPGAIRRATEECLRRVSFERKLRLLGVRASTLSRGNSCQADTPRQKELPLAG"
     gene            complement(4569..7097)
                     /locus_tag="VFI43_07790"
     CDS             complement(4569..7097)
                     /locus_tag="VFI43_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7062065.1"
                     /translation="MARVFALTFMNLVKLSFRMLRRDWRAGELRVLALALIIAVGGMT
                     TVGFFADRVQLALSRQGNQLLGADLIVFSDHPLAGKYAEEAKRLGLATSTALKFPSMA
                     AKGDNSLLTEIKAVTAGYPLRGDLRITEDFSKASPETARTADFIPSPRTAWVDEKLMV
                     RLDLKRGDKIELGATRLTVTALIAQEPDFSIGFINLRPRLMINAADLPATGLVQEGSR
                     ISYRLLVSGESGKVEHFRTWAQSQLTLGERIEGIRDARPEIRAALERAEKFLSMAALA
                     SVVLAAAAAALAVRRFTQRHLDGCAVMRCLGASQPSIFRLYLYHFITLGVIASIIGCL
                     LGFIAQQALAFWLSEMVEAELPSPTIWPAVHGILTGIVLLLGFSLPPLLNLRSVPALR
                     VLRRDIGMPNTYSMAGYVLGMAALSALFLWKAGNVRLGVSVIGGFIAAIAVFGSVGYL
                     LVKGLAHLRGQTGGAWRYGLANIRRRATSSVVQAVALGLGIMALLALTLIRDDLLQNW
                     RTSLPPDAPNHFLVNIQKDQLEPLEAFFAQRHMAHPPIFPMIRGRLTAINDTPISSGD
                     FSDIRARRLVEREFNLSWAERMQNDNQIVKGRWWKDTDRGKPVLSVEEDIAKTIGINV
                     GDTLTYDIAGSTFTAKVTSLRKVNWDSFKVNFFVVTPPGVLEDYPASYITSFYLPPHQ
                     TELTNQLIKAFPNLLIIDVASILAQVQKMIEQVTKAVEFVFLFTLLAGLAVLYAAIVS
                     TQDERIREAAIFRALGAKRRQLARAWAAEFAILGGLAGLFAAAGASALGYALGEYALN
                     LTYTFDPWLWLIGVVAGMTGVTAAGLIGTHSALSTPPLMTLRKV"
     gene            complement(7227..7628)
                     /locus_tag="VFI43_07795"
     CDS             complement(7227..7628)
                     /locus_tag="VFI43_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7062066.1"
                     /translation="MTSITSARDMPEKRSDHKTAFERSPCPITNGLDIFGDKWSLVII
                     RDLMLGKRRYQDLISSPESIASNILADRLRRLEVAGLVERRPYQQTPVRYEYILTEKG
                     RDLKPVLEAIIEWGKRHYPDTVVFAPFSPAE"
     gene            7907..8611
                     /locus_tag="VFI43_07800"
     CDS             7907..8611
                     /locus_tag="VFI43_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7062067.1"
                     /translation="MLTLYQFERTWGIPNLSHFCCKTETYLRMAGIEYAIKTTLPLAA
                     PKGKLPYIEDGDLKLADSRFIIEHLKTRYRDLDVELTPAEAALSLGMQRLIEEHLFWA
                     TMYSRWHYTDSNWQVNKKAIFSVLPPVINNLAALFYRYRIKQQIYGHGMGRHKPEEVF
                     ELGMKDLDALSACLGDKHYFLGEHPTTLDASAFGFLINTIGCPIESPLKDYGLAKRNL
                     RNYVDRVKAKYYPDLA"
     gene            8681..9310
                     /locus_tag="VFI43_07805"
     CDS             8681..9310
                     /locus_tag="VFI43_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062068.1"
                     /translation="MGLWRKLSPRTRLLAVAGLVGLIVVGGTQFHVSSQGDRSSTYPK
                     GYRGGTCTIETEGLMVGYSAYFIPSDYEIPDDPTSAKAVPVLCGMVPSPGTLNVTLDL
                     LHPESARNALLGLRLVKLIESGGEQELLSVPPQAYRSGTLTQAFKFDATGQYMLYLDG
                     KQSDAAGFNVQIPIKVGTDWRDYAAKYWIQALIFVFVALVLGNWKRIFG"
     gene            complement(9370..11265)
                     /gene="atzF"
                     /locus_tag="VFI43_07810"
     CDS             complement(9370..11265)
                     /gene="atzF"
                     /locus_tag="VFI43_07810"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HET7062069.1"
                     /translation="MTSSAVDRLGDIPSLLRRYADGELTPRKVVESVHAMIADDPDHI
                     WIHKLPLEAMMAFAQQLEPRVEEISALPLYGVPFAIKDNIDLAGVPTTAACPAFSYTP
                     QRNATVVQRLIDAGAIPVGKTNLDQFATGLNGTRSPYGACRNAFEPEYISGGSSSGSA
                     VALAKGLVSFSLGTDTAGSGRVPAAFNNLIGYKPTRGWISTNGVVPACRSLDCVSVFA
                     FTACDAGRVIEVAAAYDAEDIYSRAPRIKGYRFDFNRFCFGIPRKAQLRFFGNSESER
                     LFNEAVARLEDLGGEAIEVDLDPFLEAAHLLYGGPWVAERYVSIQPFFDLHSDQVIEP
                     VRGIIADAKRFSAADTFKGLYRLRQLKRQADQIWIDVECLLTPTTGTIYTINEMLRDP
                     IGLNSNLGYYTNFVNLLNYAAVAIPAGFQNDGLPAGVTLIAPADHDVALLDLAGHLQN
                     AYALPLGATGIPLEEASLPERLGMPTLREPFVIPASQPVAPLSDSSDSIGRVRVAVCG
                     AHLTGLPLNDRLTNRDGQLITATMTSTDYKLFALPGGPPHRPGLVRVEKDEQGYAIEV
                     EVWELPLGEFGSFVAEIPAPLGISTITLANGETVQGFVCERYAVTEAEDISRSGGWRK
                     YLSQNVE"
BASE COUNT         2552 a         3226 c         3118 g         2491 t
ORIGIN      
        1 cgcatgcata gccgattcct cgggtcttga aagagccgga tggttgcgcg gactccattt
       61 tcagccaaat ggattgcccc actttatgac taatcgaagg agattcaatt aatggcgttt
      121 caatatgtag cggcattcaa agagagatgg ttgaaaggtg atatgacttg aagctgacgg
      181 cgtacaggat gcttgtttct cccaaaatgg gaaagtatgc tcatgaactt tttccaattc
      241 tactatggat gcagcaactg caatacccca cggcgtccca atcaggggag taagtcctta
      301 aactgctgca aatagatgag gtgaggtagc cgccgctttg gttcggtaag atgacgttta
      361 tcgagacgtt gatcattact gaaagggaaa gcggttagta tgggacggga attattgcca
      421 gggttattga gtggtgagga agggctttcg aagctggtgg aagcggtgct cagtcaggta
      481 ttggaggccc agtgagttgt ttctctgaat ggtaagattt ccccttccag agaaaagcga
      541 tcagccgaga tgcataaacg ggagccgaac tgattcacgt atagcggagg cgccgccagg
      601 aaggacgtgt tttacctgcc cgaaacgccg ttcaattggc gagagaagat gtgccttggc
      661 atctatcgga ggaatgggct aatgctcgaa actgaaaagc tcgataagct gtcctgtctg
      721 ttgaaagaga tgactcgtcc cggaattagc caaccaaagg ccgcaccgct cctaatcatg
      781 gagcgccaga tagcgcactc aatgatcggg ttcttactcc tcctcggatt cgcgtatttt
      841 tcgcagtctg aaggacagcg gctctttggc attacgcctt tggaactgca tgatgctctg
      901 cttattgtcc agtggccctt aatagcttgc ttcgtcttct ttctggtgat gcttacccgc
      961 cgcgcccttt acatcagacg gcaccgcaaa gagcttgttc cctttccctt tttgtatccc
     1021 ccgcagaaaa gtgctttgca tcgcgacgcc gaattcatta cacagttatt gaggttcgac
     1081 aaagcaacac tagcttatgg cctacttcaa tacagtcatc gctggtcgtc cccggagggc
     1141 cgggccgcag tattcgttgg tgacttgcgg aagcttgggc tagtcccaac agtggtcgcc
     1201 ctattcatat ctgtagcaat gttgttcaag ggggatagca gtcctttcct tttattcctt
     1261 caggaccttg tggtaatcgt cataatctac tacgtggcgg cattccttgg ctttctttcc
     1321 cgtgaaaggc cccagcaagt cattcaattg ttggagtacg ccattcagca cgcggatcaa
     1381 tgcaacgcta ccccacacga tgccatcgca agtgcacctt ctgattaggg tgtcccgtga
     1441 aggggcctct tttgcaacac taaaacgagc actgtgaatt agactggtgt gtcacctaca
     1501 tagctcttgg cgcatcaccc gcccgaacgg cccttgaatc cccagtagga cttaccacga
     1561 cgcactgttc ctgcttgcag agtgtgtgtt gtcgcacggt aacgtttccc actacgcggt
     1621 aggctctcta gtacagcaat atcgctgtgc cgaaaaaagg aactatcatg aaatactatt
     1681 ccccgttgaa aagcaaaggg atgctgccgg ttctcggcgc ggtcttgttg accatggcgg
     1741 gagtcgccca cgcgcagatt ccaacgccgg gccaaggcga cgcgatgcaa aggccggcgg
     1801 actcgatgca gcccccggtt attcccggca aaccattcga gaaagggacg acgagaggcg
     1861 aagaagcaca ggcacagaac cggttcaata aaaacgaaga agaagccatg cgccacaagc
     1921 tggatacgaa taaaggttat ggcagcaggc aggacggcgg ttctggctca agctccggcg
     1981 gctattgatc tgcgaggcct tcggcggcgt ttccagtttg ggttcgacag atcgtgtgac
     2041 cgggggcgcc gttctttttc gagatggcat gtcgtcaggg ggaagttttt ggcctgacct
     2101 gcatgcctgc ttttacatcc tggtcagatg aaagcagtat ccggtcgccc tccttcaagc
     2161 cactgattac ttcggtaaac gcccagttgg caagacccgt ttccaccgta cgttcctcga
     2221 gcctgctctc ctgattcacg accaagacct tgtcattctg cctgatcgcc tgcgtgggca
     2281 cgcgcaatac gttttcccgc cgctgaatga cgacctcgac gtctgcgctg tatccaacca
     2341 gcaacgcgtt cttcggtatt tcctcaaagt tgatttccac gtccactgtt cttgcctgtt
     2401 tctccacctc ggtaacataa ggcgcgatgc gacggacttt tcccggaaat gattgtccgg
     2461 ggagggcatc cagtgtaatg cgggccgact gccctacctt taactttggt gcatcgacct
     2521 catccatcgg cgccgtcaca tagagacacg tatcgtcgat caagtccacc gccggtggcg
     2581 ttgggattcc cggtggcgat ggggtggtat attcgccaag ttcgccggta atcagcgcga
     2641 cgatgcccgc gaaaggcgcg acgagtactg tgcgctccaa tccagcctgg gtgaccttca
     2701 cttccgcttg cgcgcgtttc acatccgaag cggtcgcgtc gcaactggct tgacgggcac
     2761 gggcttctgc gtcggcattc tcactcgctt gaggactgag aaaacccttt gcggcaagat
     2821 ggtgggtgcg ttcagcttgc cgccgcgcat tatctgccac gatacaagcc tcccggcgcc
     2881 gctccttcgc ggtcgccagg tgtcgctggg ccaagtcgcg ctgtgccgca atgtctgaat
     2941 tccacaattc cagcaacggt tgcccctttc ccacccggtc gccttccttt acccacagct
     3001 tgacaatctg ccccccggcc gcaggcgcga gcttggcccg gcggcaggct ttgatcgtcc
     3061 cggcacgcgt attgacagca gtcgcctcca ccacgccccg gctgatcgtc cccagctcaa
     3121 cctccaccgg ctttggtcgc gtggcgaacc agacggcgaa accgagcacc gcaagtatca
     3181 gcacagcaac cgtcaggcgg atcaatattt ttgtttttat catgacatac gtaacacgga
     3241 acaagacgga tgcgctgccc tcgccgaagc tcctttagcc agccagcggc aattcctttt
     3301 gccggggcgt gtctgcctgg caggaattcc ctcgtgataa agtgctggcg cgcacgccca
     3361 gcaatcgcag cttccgttca aaggatacgc gccgaaggca ttcttcagtt gcccggcgga
     3421 tggcgcctgg atcagcggtg ggagaggata atgtaatatt ccgcgttata gtatggaagt
     3481 cgtcataccg cagcttgatc ccgatagcgc ggctgacata ccctttgcgc cgcagatcgc
     3541 ctgccagttc cctgcacagc gccgtgagaa tttccgaaag cacgtgcctg tccgcgcgtg
     3601 agtgaagatc gcgctcgaaa gtagtctccc ggctgagcga cttgggttcg gagtctgtga
     3661 tgacgggccg gtcatcgata ccattagcag cctcatgcag ccaattcccg tagctcctgc
     3721 caaattgcgc ctgcaggaag gatctgtccg cgtttgcaag gtccgcgatg gtaacgatgc
     3781 cgagcgctga aagttttccg tttgctttag gaccgacacc gctgatcttt ttgaccggca
     3841 ggggccagat cttttccggc atatcgtccg ggccgagaat gacaagtccg tcgggcttat
     3901 caaggtcgga acagattttt gcgagcagct tgttcgttgc aacgccaatc gaacaggaga
     3961 gcgcggtgct ttcccgtacg gcctgcttga gccgccgggc aagggtggcg gcgtcctcgc
     4021 cgagttcgct tagatcaatg taaatctcgt caattccatg atcctcgata cgaggcgcga
     4081 tagcggcgac cgctgccttg aacagccgcg aatagtggcg ataggcatcg aagtcggcgg
     4141 gaagaagaat ggcatccgga gcgagttggg ccgccttcat gagccccatt ccggagaaaa
     4201 tgccgaaaac gcgtgcttca taggttgccg tcgttacgac accccggccc gcatagcttt
     4261 taaggcgcaa gaaccggctg ccgctgcctt caagagcttc tggatccgcg tctccgcgcc
     4321 ccccgacgac gacgggcagc ccccgcaact ccgggtagcg gagcaactcg acggatgcat
     4381 aaaatgcatc catatccaga tgagcgattc gacgagacac tacacgtact ttgatcatcc
     4441 ccgcaatcga ctgcaccctc ccaaggccac gatgatcgca agaggtgctg gtggaaaggg
     4501 tctctcgtag cctggctttc ggccctcgcc cgaggccctc tcaaggtggc gagggaagcc
     4561 agggcacatc aaaccttccg caacgtcatg agcggaggtg ttgaaagtgc ggagtgagtt
     4621 cctatcaagc ctgccgcggt aacccccgtc ataccggcca caactcctat gagccacagc
     4681 cagggatcga atgtgtaggt cagattcagc gcgtattcgc ctaaggcata gcccagcgca
     4741 ctggcgcctg ccgcggcaaa aagcccggcc agcccaccga gtatcgcgaa ttcggctgcc
     4801 catgcgcgcg ccaactgccg ccgcttcgcg cccagcgcgc gaaatatcgc agcctcgcgg
     4861 atacgctcat cctgggtgga gacaatcgct gcgtagagca cagcgagccc agccagcagc
     4921 gtaaacagga agacaaattc aaccgccttg gttacttgtt cgatcatctt ctgtacttgc
     4981 gccaggatcg acgcgacatc gattatgagc aaattcggga aggccttgat cagttgattc
     5041 gtcagctcgg tctgatgagg cggaaggtaa aaactcgtaa tgtagctcgc cgggtagtct
     5101 tccagaacgc cggggggcgt aacgacaaaa aaattgacct tgaacgagtc ccagttcact
     5161 ttcctcaaac tggtgacctt ggccgtaaac gtgctgcccg ctatatcgta ggtaagcgta
     5221 tcaccgacat tgatgccaat cgttttcgcg atatcttcct cgacagatag aacaggtttg
     5281 cctcgatcgg tgtctttcca ccagcggccc ttgacgatct gattgtcgtt ttgcatgcgt
     5341 tccgcccatg acaggttgaa ctcgcgttcc accagccgcc tggcacgaat gtcggaaaaa
     5401 tcgcccgatg atatgggagt atcgttaatt gccgtgagcc gtccgcgaat catcgggaaa
     5461 atgggtggat gcgccatatg ccgttgggca aagaatgcct cgagtggctc aagctggtct
     5521 ttctgaatgt tgaccaggaa atgattggga gcgtccggag gcaggctggt acgccagttt
     5581 tgcagcaggt catcccgaat cagggtcagc gccagcagcg ccattatgcc aaggcctagc
     5641 gccactgcct gcaccacgct tgaagtcgcc cgcctgcgga tgtttgccaa tccatagcgc
     5701 catgccccgc ccgtttgccc acgaaggtgg gcgagtcctt tgacgagcag gtatccaacc
     5761 gagccgaaca ctgcaatggc cgcgatgaac cctcctatga cggaaacgcc caagcgcaca
     5821 ttcccggctt tccatagaaa cagcgccgac agagccgcca taccgagtac atacccagcc
     5881 atgctatagg tattgggcat gccaatatcg cgtcgcagta cccgcagcgc ggggacgctg
     5941 cgcaagttca gcagcggcgg caaggaaaag ccgagcaaca agacgattcc cgtcaggatg
     6001 ccatgcacgg ctggccagat ggtaggcgac ggcaattcgg cttctaccat ttccgatagc
     6061 cagaacgcca gcgcctgctg agcgatgaaa ccgagcagac agccgataat gctggcgatc
     6121 accccgaggg taatgaaatg gtagagatac agcctgaata ttgaaggctg gctcgcgccg
     6181 agacaacgca tgaccgcgca gccatcgaga tgtctctggg taaagcggcg cacggcgagc
     6241 gctgctgcag ccgccgccag tacgacgctg gcaagagcgg ccatgctcag aaatttttcc
     6301 gcgcgctcca gtgctgcacg gatttcaggc cgggcatcgc ggataccttc gatacgttcc
     6361 ccaagcgtca gctgcgactg agcccatgtg cggaaatgct ccaccttccc ggattcacct
     6421 gaaacgagca gacgatagct gatgcggctg ccttcctgca cgaggcctgt tgccggcaaa
     6481 tccgcggcat tgatcatcag gcgggggcga aggtttatga agccaatcga gaaatccggc
     6541 tcctgcgcga tcagtgcggt gacagtaagc cgagtcgccc ccagttctat cttgtcgccc
     6601 cgcttgagat cgaggcgaac catgagcttt tcgtccaccc aggccgtgcg gggagacggg
     6661 atgaagtcag cggtgcgggc agtttcggga gatgctttgc tgaagtcctc ggtgatacgc
     6721 aaatcgcccc gcagcggata accggcggta acagctttta tttccgtgag aaggctgtta
     6781 tcccccttgg ccgccatgct gggaaacttg agcgcggtag aagtcgccag ccctagccgt
     6841 tttgcctcct ccgcgtattt cccggccagt ggatgatctg aaaaaacgat cagatcagcg
     6901 ccgagtaact gattgccctg ccgggaaagc gccagctgaa cgcggtcggc aaaaaagccg
     6961 acggtagtca ttccgccaac ggcgatgatc agggcaagcg ccaggacgcg cagttcaccc
     7021 gcgcgccagt cgcggcgaag cattcgaaaa gagagcttga caaggttcat aaacgttagg
     7081 gcaaataccc ttgccatgct tccaactgaa gcgtggcaga gtcattcagg atggaaatcc
     7141 atgcaagcag ggttttgttc agctatcgga atcgcatcgc ccgcctggat ttattggtgc
     7201 aagcgccaca gtgtatggag caagcgctac tcggccggtg agaatggagc gaatacgacc
     7261 gtgtcggggt aatgcctttt accccattca ataatagctt ccagcaccgg cttcaggtcc
     7321 ctgcccttct ccgtcagtat gtattcgtag cgtaccgggg tctgctggta gggccgccgc
     7381 tcgaccaggc cggccacttc cagtctcctc aagcggtcgg ccagtatgtt cgaggcgata
     7441 ctttccggcg aggatatgag gtcctggtag cgccgcttgc ccagcatcag gtcacggata
     7501 attaccagtg accatttgtc cccaaaaata tccagcccgt tggttatagg gcagggcgac
     7561 cgctcaaatg ctgttttgtg atctgatcgt ttttccggca tatcgcgcgc gctggttatg
     7621 ctggtcatgt tttactcgaa taactgccca tcggataatg taatcgactc acttgcaaat
     7681 tgcaatatat caggctaagc tcacgttgct ttcgaactgc aagccaagag cgcgaaagtt
     7741 atgaacgcga attccgcagc gagttccagc cagcgaacgg cttgctgcag attgcggcgg
     7801 attgcttgtc ccgatgcaga ttatgagcgg cagtttctga cctcattcaa attggctgtc
     7861 atattcatat ttcaaatcca ttcccgccgg gaaattcata tttcatatgt tgacacttta
     7921 ccagttcgag cgcacttggg gtattcccaa cctgagccac ttctgttgca agacggagac
     7981 ctatctgcgc atggcaggca tcgagtatgc cattaaaacc accctcccgt tggcggctcc
     8041 caagggaaaa ctgccctaca tcgaggacgg cgacctgaaa ttggcggact cgcgcttcat
     8101 cattgaacac ctcaagacca ggtatcgaga cctggatgta gagttgactc cggcagaggc
     8161 cgcgttgtcg cttggcatgc agcgattgat cgaggagcat ctcttttggg cgaccatgta
     8221 ttcgaggtgg cattacacgg attccaactg gcaagtcaac aaaaaagcca tattcagtgt
     8281 cctgcctccc gttatcaata acttggcggc tttgttctac cgctacagga ttaagcagca
     8341 gatttatggt catggaatgg ggcgtcataa accggaagaa gtttttgaac tcgggatgaa
     8401 agaccttgac gcgctatcgg cgtgccttgg ggataagcac tatttcctgg gtgagcaccc
     8461 gaccacgctc gatgccagtg ccttcggatt cctgatcaat acgatcggtt gccccatcga
     8521 atcccccctg aaagactatg gtctggccaa gcgcaatctg cgaaactatg ttgacagggt
     8581 caaggcaaag tattacccgg atctagcttg atgagcactg ccggggccat gctcgaaaaa
     8641 gacaacgcgc atgatggaaa atggcagagg aacaaccagt atgggacttt ggcgcaaatt
     8701 atcgcctcgt acgcgacttt tggcggtagc ggggctcgtc ggactcatcg tggtgggggg
     8761 cacgcaattt catgtgagtt cacagggaga ccggtcttcc acctatccca aaggatatcg
     8821 cggcggaacc tgcacgattg aaactgaagg gttgatggtc ggctactcgg cctacttcat
     8881 ccccagtgat tatgaaatac ccgatgatcc aaccagcgcc aaggcggttc ccgtcttgtg
     8941 cggcatggtg cccagcccgg gaacgttgaa tgtaacgctt gacctgctcc atcccgagtc
     9001 cgcgcgcaat gcccttctcg gactccggct ggtcaaactg atcgaatcag ggggcgagca
     9061 ggagctgctt tcagtgcccc ctcaagccta ccgatccggt acgctgaccc aggcgttcaa
     9121 atttgacgca accgggcaat acatgctcta tctggacgga aaacaatcgg atgcggctgg
     9181 cttcaatgtc cagataccga tcaaggtcgg caccgactgg agagattatg cagccaagta
     9241 ctggatacag gctctaatat tcgttttcgt tgccttggtt ttaggcaatt ggaagaggat
     9301 attcgggtaa ttactcaaaa gtaggttggg tggatatcct caaagcggct tttcgggatg
     9361 ccttggtgcc tattcgacat tctggcttaa atacttgcgc caccctccag accggctgat
     9421 gtcttccgcc tccgttacgg catagcgttc gcagacaaaa ccctgtactg tttcgccgtt
     9481 tgcgagggta attgtactga tgccgagtgg ggctggaatc tctgcaacaa agctcccgaa
     9541 ttcccctaga ggcaattccc agacttccac ttcaatcgcg tatccctgct catccttttc
     9601 cacacgcacc aaccctggac ggtgaggagg tccccctgga agcgcaaaaa gcttgtaatc
     9661 tgtcgaggtc atggtcgcag taattaattg cccatcccgg ttggtcaatc ggtcattcag
     9721 gggaagaccg gtcaaatgag cgccgcagac tgcaactctc acccggccta tgctatccga
     9781 ggaatcggaa agcggcgcga caggttggga tgccgggatg acgaatggct cgcgcaacgt
     9841 tggcattccc aaacgttctg gcaaacttgc ttcttccaat ggaatacccg tagcaccgag
     9901 gggtagtgca tatgcgtttt gcaagtgccc ggcaaggtcc agcagagcta catcatgatc
     9961 agctggtgca atcagtgtta ccccggcagg caacccgtca ttctgaaatc cggcagggat
    10021 tgctacggca gcatagttca ggaggttgac gaaattggta tagtagccaa ggttcgagtt
    10081 aagtccgatg gggtcgcgaa gcatctcatt tatcgtgtag atcgtgcccg tcgtcggtgt
    10141 aagaaggcat tccacgtcga tccatatctg gtcggcctga cgtttaagct ggcgcagccg
    10201 gtaaagccct ttaaaggtgt ccgctgccga aaagcgtttc gcgtcagcaa taattccgcg
    10261 caccggctcg atcacctgat cgctatgcag atcgaaaaac ggctggatgg acacataacg
    10321 ctccgccacc caaggcccgc catagagcaa gtgcgcggct tcaagaaaag gatcgagatc
    10381 aacctcgatt gcctctccgc ctaaatcttc cagccgcgcc accgcctcat taaacaaccg
    10441 ttcggattca ctattgccaa aaaagcgcaa ttgcgccttt cgagggatgc caaagcaaaa
    10501 ccggttaaaa tcgaaccgat aacctttgat cctcggcgcg cgcgaataga tgtcctccgc
    10561 gtcatatgcg gcggctacct cgatgaccct cccggcatcg caggccgtaa aagcgaaaac
    10621 cgaaacacaa tcgagagaac gacaggctgg cacgacgccg ttcgtggaaa tccagccccg
    10681 cgtcggctta tagccaatca aattgttgaa cgccgcaggc accctgccgg accccgcagt
    10741 gtcagttccc agactgaaac tcaccaagcc cttggcaagc gcaacggcgg agcccgagct
    10801 cgatccgcct gaaatatact ccggctcgaa tgcgttgcgg caggctccat aaggcgagcg
    10861 cgtgccattg agaccggttg caaactggtc gaggttggtt tttccgacag gaatggcgcc
    10921 tgcatcaatc aatctttgca ccacggtcgc gttgcgctgg ggggtatagg aaaacgcggg
    10981 gcatgccgcg gtagtcggca cacctgccag atcgatgttg tccttgatgg caaacgggac
    11041 accatacaac ggtagcgccg aaatttcctc gacccgtggt tccagctgct gggcaaaggc
    11101 catcatcgcc tcaagcggca gcttgtgtat ccagatatga tcgggatcat ctgcgatcat
    11161 ggcatgcacc gactccacga ccttgcgggg agtcaactcg ccatcggcat agcgccgcag
    11221 caacgatggg atatcaccca gcctgtcgac cgcggaactt gtcactgggc ctcctgaatc
    11281 acacacagca tctgcccggc tgacacatgc gtgccttcct tgcaaaaaaa ctggcctacc
    11341 atgccgtcga ccggcgcgct caccggaaac tccattttca tggactc
//
LOCUS       DASSHJ010000200         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_822021, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000200 DASSHJ010000000
VERSION     DASSHJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822021"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..499
                     /locus_tag="VFI43_07815"
     CDS             104..499
                     /locus_tag="VFI43_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET7062070.1"
                     /translation="MAELTDDQLAQLKTRLQDLYRTLREGVRTEIEDADGQHYEDLAG
                     AVPDPGDSSVADVIVDTGAALTDKQVNELREVEAALKRLADLDYGDCEECGGEIGFER
                     LMAYPTATRCIRCQEVHEKTYAHENKPTM"
     gene            complement(626..1243)
                     /locus_tag="VFI43_07820"
     CDS             complement(626..1243)
                     /locus_tag="VFI43_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7062071.1"
                     /translation="MEAPSQASGVPASPAATKIEEDEAALFRNSVRDVSPLPPPDKAS
                     KSAKARPPPLPRPVLQSESKQPSDFTSSDFLVGIEAGEEVSFSRPGLARQTLRRLRRE
                     HWGVDAELDLHGYTRDQAQGELKAFLDNSVSRGFRCVRVIHGKGLGSRNHEPVLKARV
                     GGWLKQRNDVLAFCPARPEHGGSGAVLVLLRSSRGEHNDYSGYKS"
     gene            complement(1411..2364)
                     /gene="trxB"
                     /locus_tag="VFI43_07825"
     CDS             complement(1411..2364)
                     /gene="trxB"
                     /locus_tag="VFI43_07825"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7062072.1"
                     /translation="MTTNKHARLIILGSGPAGYTAAVYAARANLKPVIITGLAQGGQL
                     MTTTDVDNWPADAMGVQGPELMDRFQRHAERFNTELIFDHIHTAKLLEKPLTLIGDQG
                     TYTCDALIIATGASARYLGLPSEEAFMGKGVSACATCDGFFYKGQDVAVVGGGNTAVE
                     EALYLSHIARNVTVVHRRDKFRSEKIMIDKMMEKVASGNISLELHYVLDEVLGDQSGV
                     TGMRIRNVQDDSVKEIKLQGVFIAIGHKPNTDIFEGQLKMENGYIMTRSGNHGNATAT
                     SVPGVFAAGDVQDHIYRQAVTSAGTGCMAALDAEKYLDLLA"
     gene            complement(2433..>2725)
                     /locus_tag="VFI43_07830"
     CDS             complement(2433..>2725)
                     /locus_tag="VFI43_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7062073.1"
                     /translation="DADSKTLRETLDKLKNKLQPCVVVLSSVEGNSVKLIAGVSSDLT
                     AKVKAGELVNFVARQVGGKGGGRPDMAQAGGTLPDELPTALESVTGWVEQRL"
BASE COUNT          574 a          792 c          754 g          605 t
ORIGIN      
        1 gccccggtta cctatacttc gattggaact gaagattgca caaccgtttt gcagtctcaa
       61 tctccgtatt ccagcgcctt agaacattcc aggacaaacg acaatggcag aactgacaga
      121 cgaccagctt gcgcagttga agacgagatt gcaggacctg tatcggacgc tgcgggaagg
      181 cgtacgcacg gaaatcgaag acgcggacgg ccagcactat gaggatctgg caggggcggt
      241 gcctgatccg ggcgacagct ccgtggcgga tgtgatcgtg gatactggcg ccgccctgac
      301 agacaaacaa gttaatgagc tacgcgaggt ggaagctgcg ttaaagcgtt tggcggacct
      361 tgattacggc gactgcgaag agtgcggcgg ggaaatcggc tttgagcgtt tgatggctta
      421 tccgacggcg acgcgatgca tacgttgcca ggaggtgcat gaaaagacgt acgcgcacga
      481 gaacaagccc acgatgtaat gctatctggc tgtcagtccc tcaatacggt taaatgtcgc
      541 taggtaatgt aaataacccg cggtaggaat agtccatagg aataagccac agggagggtc
      601 acagggagta caggcaggcc gggcatcaac ttttgtagcc gctgtaatcg ttgtgctcac
      661 ctctggatga cctgaggagt accaataccg cgccgctccc gccatgttcg ggtcttgccg
      721 ggcaaaatgc cagcacgtcg tttcgctgct tgagccatcc gcccaccctg gctttaagaa
      781 ccggctcgtg gttcctggag cccaagccct tcccgtgtat cacgcgaacg cagcgaaaac
      841 ccctgcttac gctgttatcc agaaacgctt tcagttcacc ctgcgcctgg tcccgcgtat
      901 aaccgtgcag atcaagttct gcatcgacgc cccaatgctc gcgtcgcagc cgccgtagcg
      961 tctgccgcgc caaacccgga cgcgaaaagg atacctcttc gccggcctca atcccgacga
     1021 gaaaatcact ggaagtaaag tcgctcggct gcttcgattc actctgcaaa accgggcgcg
     1081 gtaaaggcgg cgggcgcgct ttggcggatt tgctcgcctt atctggcggg ggcagcggag
     1141 acacatcccg gacagaattg cgaaacagtg cggcttcgtc ctcctcgatt ttggttgcag
     1201 caggagatgc aggcacgccg ctggcctgag atggcgcttc cacttccgtt gacggtgtcc
     1261 ttccgccgcg tttcatcgct tgaaatataa aagaattgag ctgcgattgc gcgattgtga
     1321 tgccaagcct gcggatgttc agctggttac ccgtaatggg atgccaccct ctcccccacc
     1381 ccctctccca cttgtgggag aggggagttc ttatgccagg agatccaggt acttttccgc
     1441 gtccagcgcg gccatgcacc cagttccggc gctggtcacc gcctggcggt agatgtgatc
     1501 ctgcacatcg cccgcggcga aaacaccggg aacgctcgtt gcggtcgcat tcccatggtt
     1561 gccgctgcgc gtcatgatat aaccgttctc cattttcagt tgcccctcga aaatgtcggt
     1621 attcggtttg tggccaatcg cgataaacac gccttgcaat ttgatctcct tgacagagtc
     1681 atcctgcaca ttcctgatgc gcatcccggt tacacctgat tgatccccaa gcacttcgtc
     1741 gagcacgtag tggagctcca gggaaatgtt gccactggcg accttctcca tcattttgtc
     1801 gatcatgatc ttttcggaac ggaatttatc gcgccgatgt acaaccgtca cattccgcgc
     1861 gatatgcgag agatacagcg cttcttccac cgccgtgttc ccgcctccta ccaccgcaac
     1921 atcctgtccc ttgtaaaaaa atccgtcgca cgtggcgcaa gcggacacgc ctttgcccat
     1981 gaacgcttct tccgatggta atccgagata acgcgccgag gcgcctgtgg cgataatgag
     2041 cgcatcacat gtgtaggttc cctggtcgcc gatcagggta aggggctttt ccagcagctt
     2101 ggctgtatgg atgtgatcga aaatgagctc ggtgttgaaa cgctcggcgt gtctttggaa
     2161 ccgatccatc agctccggcc cttgaacgcc cattgcatcc gccggccagt tgtccacgtc
     2221 ggtagtcgtc atgagttgcc cgccttgcgc gaggccggta atgatgacag gcttgagatt
     2281 ggcccgggcg gcgtagaccg cggcagtgta tccggcgggg ccggagccga gaataataag
     2341 acgcgcgtgt ttgtttgttg tcattgcttg aagtcaggat gtgcttgaaa caaaaagctg
     2401 ctggctcaaa tggatcgaaa aactgaaaag ttctacaacc gttgctcgac ccaacccgtt
     2461 acgctttcca gcgcagtggg caactcatca ggcaatgtac cgcctgcctg agccatgtcc
     2521 ggccggccgc ccccttttcc gcccacctgc cgggcaacga aattaaccag ttctccagct
     2581 ttcaccttgg ccgtcagatc agagctgaca cccgcgatca gtttgaccga gttgccttct
     2641 accgagctca gcaccacgac gcacggttgg agtttgttct tgagcttgtc cagtgtttcg
     2701 cggagcgttt tcgaatcggc atcct
//
LOCUS       DASSHJ010000201         8429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_792915, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000201 DASSHJ010000000
VERSION     DASSHJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792915"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFI43_07835"
     CDS             complement(<1..628)
                     /locus_tag="VFI43_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7062074.1"
                     /translation="MSPFSPPPPRVGLVLTGGGARAAYQVGVLQAIATMLPPRTRNPF
                     PVICGTSAGAFNAAGLALSARSFHRGVRRLSAVWENAHVSQAYRSDPIGVYGNAARWL
                     TSLVFGSFVKRKVISLLDNSPLAQTLEKNLPLQGIQRNIKEGALHALGITAWGYTSGQ
                     SVTFFQGGESIEPWKRERRIGIRSRIGIEHLMASSAIPILFPAVRLNRE"
     gene            complement(625..2037)
                     /gene="fumC"
                     /locus_tag="VFI43_07840"
     CDS             complement(625..2037)
                     /gene="fumC"
                     /locus_tag="VFI43_07840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET7062075.1"
                     /translation="MNYREERDTMGVIQVPANALWGAQTQRSVQNFRISGERMPLELI
                     RALAMVKRAAASVNQDLGLLRSDVAAAIITAADEVISGKHDGEFPLVVWQTGSGTQTN
                     MNMNEVIANRASELLGGGRGITSKVHPNDEVNKGQSSNDVFPSAMHVAAVRAICQELQ
                     PAILTLRQTLATKAGEFSSIVKIGRTHLQDATPVTLGQEFSGYVSQLDHSLEHVQATL
                     PHLCELALGGTAVGTGLNAHPDFAVRIAAELASMTGLPFITAPNKFEALAASDAMVYA
                     HGALKTLAASLIKIANDIRWLASGPRCGIGELKIPENEPGSSIMPGKVNPTQSEALIM
                     LCCQVMGNDVAINMGGAMGNFELNVMRPLIIYNFLHSVRLLADGMRSFNDHCVSGITP
                     NRERIDALMRNSLMLVTALTPYIGYDKAAAIAKKGHQEGTTLKEAAVTSGYVTPEQFE
                     AWVKPEYMVGSKRSDLALDG"
     gene            complement(2332..2607)
                     /locus_tag="VFI43_07845"
     CDS             complement(2332..2607)
                     /locus_tag="VFI43_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062076.1"
                     /translation="MARTIPQASRDYENTSVIERPDGFYWHDKENDKVFGPFPTLLEA
                     IQDMEYNAESDYEPGETLEEAEEEIGISDWIDQETGQPGEESFRLEN"
     gene            complement(2765..3541)
                     /gene="pssA"
                     /locus_tag="VFI43_07850"
     CDS             complement(2765..3541)
                     /gene="pssA"
                     /locus_tag="VFI43_07850"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET7062077.1"
                     /translation="MMHDPHPRLVLKSKLRRRGIYLLPNLFTTAALFAGFYAIVQAMN
                     GRFEHSSVSIFVAMVLDGLDGRVARLTHTQSEFGAEYDSLSDMVSFGAAPALVIYEWA
                     LKGMGKLGWIAAFIYCVCAALRLARFNTNIEKVDKRFFQGLPSPAAAALVAGLIWIMH
                     DFDIAGQDIRWLAWSVTLFAGLTMVSNLPYYSGKEINLRKSVPFITVLLLALFFFVLI
                     PSHPPVVLFSLFFVYAFSGYFIWLWRLIKKKRKSDVHPST"
     gene            complement(3666..4682)
                     /gene="ilvC"
                     /locus_tag="VFI43_07855"
     CDS             complement(3666..4682)
                     /gene="ilvC"
                     /locus_tag="VFI43_07855"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7062078.1"
                     /translation="MNVYYDKDADLSLIKAKKVTIVGYGSQGHAHANNLRDSGVDVTV
                     GLRKGGASWSKAEKAGITIREVAEAVKGADVVMVLLPDEQIASVYASEIEPNLKQGAT
                     LAFAHGFNIHYGQVSPRADLDVIMVAPKGPGHLVRSTYVQGGGVPSLIAVHQNPSGRA
                     RELALSYAAANGGTRGGVIETNFREETETDLFGEQAVLCGGLTALIQAGFETLVEAGY
                     APEMAYFECLHEVKLIVDLIYEGGIANMRYSISNNAEYGDITRGPRIITDATRVEMRK
                     ILHEIQVGEYAREFILENRAGAPMLKSSRRLAATHEIETVGAKLREMMPWISKNKLVD
                     QAKN"
     gene            complement(4754..5245)
                     /gene="ilvN"
                     /locus_tag="VFI43_07860"
     CDS             complement(4754..5245)
                     /gene="ilvN"
                     /locus_tag="VFI43_07860"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7062079.1"
                     /translation="MRHIISLLMENEAGALSRVAGLFSSRGYNIESLTVAATEDPTLS
                     RMTLVTVGSDDVIEQITKQLNKLIEIVKVVDLSEGDHIERELMLIKVRTVGKDREEIK
                     RLADIFRGRIIDVTDKSYTIELTGTGSKLDAFIGAIERGSILETVRTGVSGIGRGERI
                     LKV"
     gene            complement(5249..6952)
                     /locus_tag="VFI43_07865"
     CDS             complement(5249..6952)
                     /locus_tag="VFI43_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HET7062080.1"
                     /translation="MTMELTGAEITIRCLQKEGVDHIFGYPGGAVLFIYDELFKQDKV
                     KHVLVRHEQAAVHAADGYARSSNKVGVALVTSGPGVTNAVTGIATAYMDSIPLVVISG
                     QVPTYAIGLDAFQEVDTVGITRPCVKHNFLVKNVADLATTIKKAFFIASTGRPGPVLV
                     DIPKDVSQQKAPFVYPENVAMRSYNPVLKGHAGQIKKAVQLILEAKRPMIYTGGGVIL
                     SDAAAQLTELVRMLGFPCTNTLMGLGGYPATDPKFLGMLGMHGTYEANMAMQHCDVLV
                     AIGARFDDRVIGNPKHFFNEDRRIIHIDIDPSSISKRVKVDIPIVGSVPDVLAELVRQ
                     LKVRKEKTDQTALQAWWTQIESWRSRDCLRYDRASPVIKPQWVIEKLYEVTGGNAFVT
                     SDVGQHQMWAAQFYKFDKPKQWINSGGLGTMGFGLPAAMGVQFANPGEDVACITGEAS
                     IQMCIQELSTCRQYNLPIKIINLNNRYMGMVRQWQEFFHGNRYAESYMDALPDFVKLA
                     ESYGHVGMKIERPEDVEGALREAFKLKDRLVFMDFITDQTENVFPMVPGGKGLSEMIL
                     V"
     gene            complement(7317..7772)
                     /gene="fur"
                     /locus_tag="VFI43_07870"
     CDS             complement(7317..7772)
                     /gene="fur"
                     /locus_tag="VFI43_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HET7062081.1"
                     /translation="MTNSNNLKTMGLKATLPRLRILSLFESSSVRHLSAEDVYRTLIN
                     EGEDIGLATVYRVLTQFEQAGLLERHHFEGGKAVFELASGTHHDHLVCLQCGRVEEFY
                     DAEIEKRQSKIAKDRGFAIHEHSLSLYADCIKPACPYRKPVERPLGKTE"
     gene            7844..>8429
                     /gene="bamE"
                     /locus_tag="VFI43_07875"
     CDS             7844..>8429
                     /gene="bamE"
                     /locus_tag="VFI43_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HET7062082.1"
                     /translation="MRARILTLVALLLAGCSSVPSLPYKIEVQQGNVITQEMVDKLKP
                     GMTRSQVRFVLGSPMISDAFHDNRWDYVYRLEQAGRLIEQRKLVVFFEEDKLVRLGGS
                     FSPVAFNKPPSIPSAEPPKTAGALTLEPGSVSGGELPGPVTSAVPPQGTPAQGSIAAA
                     TSEQGSAAARVPVRNPVPRQVPSQATDMGDEEADQ"
BASE COUNT         1879 a         2328 c         2227 g         1995 t
ORIGIN      
        1 attcccggtt gagccggaca gcaggaaaaa gaatcggaat cgcagaagag gccatcagat
       61 gctcaatccc gatacgcgag cgtattccga tgcggcgttc ccttttccag ggttctatgc
      121 tttccccccc ctggaaaaag gtaacggact gcccggaggt atagccccag gcggtaatgc
      181 ccaaggcgtg cagggcgcct tccttgatgt ttctctgaat gccctggagg gggagatttt
      241 tttcaagtgt ttgggcaagg ggtgaattgt caagcagcga aatgaccttg cgcttcacga
      301 aactcccaaa caccaatgaa gtgagccagc gcgctgcgtt tccatatacg ccgatcgggt
      361 cggagcgata ggcttggcta acgtgagcat tttcccagac cgccgataac cgtctcacgc
      421 ctctgtggaa acttctggcc gaaagggcga gaccggccgc gttaaaggcg cccgcagagg
      481 ttccgcaaat caccggaaag ggatttcggg ttcttggcgg cagcatcgtt gcaatggcct
      541 gtaacacgcc tacctggtac gcggcgcggg ccccgcctcc cgtaagcacc aagccgaccc
      601 tagggggagg tggagagaaa gggctcatcc atcaagggcc aagtcgctcc gcttgctgcc
      661 taccatatac tccggcttga cccaggcctc gaattgttcc ggggtcacat aacccgatgt
      721 cactgcggct tccttgagcg tagtgccttc ttgatgccct tttttggcga tcgcagccgc
      781 cttgtcataa ccaatatagg gagtgagcgc cgttacaagc atcaaggagt tcctcatcag
      841 cgcatcgatg cgttctctat tcggcgttat tccagacaca caatgatcgt tgaagctcct
      901 catgccgtca gcgagaaggc gtacgctatg caggaagtta tagatgataa gcggtctcat
      961 gacgttgagc tcgaaattgc ccattgctcc gcccatattg atggccacat cattgcccat
     1021 gacctggcag cataacatga taagcgcctc tgactgcgtc gggttgactt tgcccggcat
     1081 gatggagctt cccggttcat tttccgggat tttaagttct ccaatcccac agcgaggccc
     1141 ggatgcaagc caccggatat cgtttgcaat ttttatcagg gacgcagcca gggttttcag
     1201 cgcgccatgt gcatacacca tggcgtcgct cgcggcaagc gcttcaaatt tgttaggagc
     1261 ggtaatgaac ggcaagccgg tcatacttgc gagttcagcc gctatgcgca cggcgaaatc
     1321 cggatgggcg ttcagccccg tgccgaccgc ggtgccgccg agcgcgagct cgcagaggtg
     1381 cgggagtgtt gcctgcacgt gctcaaggct gtgatccaat tgcgacacat acccggagaa
     1441 ttcctgccca agcgtcacgg gcgtggcgtc ctgcaagtgc gtgcggccaa tcttgacgat
     1501 ggaagaaaat tccccggcct tggtcgcaag cgtttgccga agcgtcagga tggcaggttg
     1561 caattcctgg cagatggcgc ggacggccgc gacgtgcatg gcggatggaa atacgtcatt
     1621 ggacgactgt cctttgttta cctcgtcatt gggatgaacc ttgctggtga ttccccttcc
     1681 tccaccgagg agttccgagg cgcggtttgc gatcacctcg ttcatattca tgttggtttg
     1741 ggttcccgac cccgtctgcc agacgacaag cggaaactcc ccatcgtgct tgccagaaat
     1801 cacttcatct gctgcagtaa tgatggcggc ggcaacatca cttctgagca gtccaaggtc
     1861 ctggttgacg ctcgccgccg cgcgcttcac catggcgagc gcacgaatta gctccagggg
     1921 catgcgctcg cctgaaattc tgaaattttg aacggagcgc tgggtctgcg cgccccacag
     1981 ggcgttggca ggaacttgta tgacgcccat ggtgtcgcgt tcttcgcgat agttcatgga
     2041 caaatggaag ataaacgggg atcagcgccc gaacggctta tcgaggcaaa gggggttctg
     2101 caagcgagat tcgtggtact gagcaaagaa gtcttatgga aactgttcgg gctcgccaat
     2161 gtatttatgg tatttataag tatttatgaa cccgagtctt ctgcctagca aaagtatggt
     2221 caccaagtgc tatgacttgg cgcgtctcta tgcgttttgg ctttcactca acagaaggga
     2281 aaacaaaaaa atcggaagaa agcaaaaaag gttgttgccg agcacttttg atcagttttc
     2341 aagcctgaac gattcctcac ctggctgacc ggtttcctga tcgatccagt ctgaaatgcc
     2401 aatttcctct tcagcctcct caagcgtctc tccaggctca taatcggatt cagcgttata
     2461 ttccatatcc tgaatggcct cgagcaatgt ggggaatggc ccgaaaacct tgtcgttttc
     2521 cttgtcgtgc cagtagaagc catcggggcg ttcgatgacg ctggtatttt cgtaatcacg
     2581 agatgcttgg ggaatcgtac gagccatggc tacctccatt acgagttacg ggtgaagaat
     2641 ctaatcttaa tctcgtcagt aaaaatttgc cagtacaaat ttgccagtac aaatggatga
     2701 ttcgaagaag caatggctcg ataccaaagg cagggtgcgc cgatgcctgg agcatgctca
     2761 ggaatcaggt cgatggatgc acatcactct ttcttttttt cttgatcaac ctccaaagcc
     2821 agatgaaata gcccgaaaac gcgtaaacaa agaagaggct aaaaagaacg acgggaggat
     2881 ggcttgggat gagtacaaag aaaaacagcg cgaggagtag aacagtgatg aaaggcacgc
     2941 tcttgcgaag attgatctcc ttcccgctgt agtatggcag attgctgacc attgtcagtc
     3001 ccgcaaatag cgtgaccgac caggcaagcc agcggatatc ctgtccggct atgtcgaaat
     3061 catgcattat ccagatgagc cctgccacca gcgcagcggc tgcggggctc ggaagtccct
     3121 ggaaaaagcg cttgtccacc ttttcgatat tggtgttgaa acgcgcaagg cgaagcgcgg
     3181 cgcaaacaca atagataaaa gcggcgatcc agcccagttt tcccatgcct ttcaaggccc
     3241 actcataaat gacgagtgcc ggggccgccc cgaacgaaac catatcggac aggctatcgt
     3301 attccgctcc aaactcgctt tgcgtatgtg tcagccgggc tacacggcca tccaggccat
     3361 cgagaaccat tgcaacaaag atggaaaccg acgaatgttc aaaacgtcca ttcatcgcct
     3421 gcacaatggc gtaaaaaccg gcgaacaggg cggctgtggt aaacagattg gggagcagat
     3481 aaattccccg tcggcgcagc ttggacttga gcacgagccg gggatgaggg tcatgcatca
     3541 ttcatcgttt ccataatggg tctgatgcat gtcaaaaggt cacctatagg gtgagagcag
     3601 aggtaccttg aactggagga aggctagaac ccgaaccttc ccgcccgcgt cttacttcct
     3661 gttctttagt tcttcgcctg atccaccaat ttattcttgc tgatccatgg catcatttcc
     3721 ctcagtttcg caccgactgt ctcgatttca tgggttgccg caagtcggcg gcttgatttc
     3781 agcatgggcg ccccggcccg gttttcgaga ataaactcac gtgcgtattc tccgacctgg
     3841 atttcatgga ggatcttgcg catttccacc cgggtggcgt cggtaatgat tcgcgggccg
     3901 cgcgtaatgt ctccatattc cgcgttgttg gaaatcgaat aacgcatgtt tgcgataccc
     3961 ccctcgtaga tcagatccac aatcaatttc acttcgtgca ggcattcaaa ataggccatc
     4021 tcaggtgcat acccagcctc taccaaggtt tcaaaacctg cctgtatcaa ggcggtaagc
     4081 ccgccgcaca agactgcctg ctcgccgaaa agatcggttt cggtttcttc gcggaagttg
     4141 gtttcgataa ctccgccgcg agtcccacca ttcgctgcgg cataggaaag tgccagctcg
     4201 cgcgcccgtc cggaagggtt ctgatgtacg gcgatcaggg acgggacccc cccgccctgc
     4261 acataagtgg atcgcaccag gtgacccggg cccttgggag caaccatgat gacatccaga
     4321 tcggctctcg gcgagacctg gccataatga atgttgaatc cgtgggcgaa tgcaagggtc
     4381 gccccctgct tgaggttggg ctcgatttcc gaggcgtata cggacgcaat ctgctcatcg
     4441 ggcaatagaa ccatgactac atccgcccct tttaccgctt cggctacttc cctgatcgta
     4501 ataccggctt tttccgcctt gctccaggat gctcctcctt tgcgcaagcc gacggtaacg
     4561 tcgaccccgg aatccctgag attattcgcg tgggcatgcc cctgggagcc atagcctaca
     4621 atagtgacct tctttgcctt gatgagtgat aaatccgcat ccttatcgta atagacgttc
     4681 atggtttcct ttctgcttaa aataaaaaac ggccagccgg ccggtcttcg tcatgtaccc
     4741 gcccgcagac cgcttatacc ttcaatatac gttcgcctcg cccaatgccg gacaccccgg
     4801 tgcgcacagt ttccaggatc gacccccgct cgattgcccc aatgaatgcg tcaagtttcg
     4861 aacctgtgcc tgtaagctcg atagtgtagg acttgtccgt gacatcgata atgcggcccc
     4921 tgaaaatatc ggccagccgc tttatctctt cccggtcttt gcccaccgtg cgtaccttga
     4981 tcagcatcag ctcgcgctcg atatggtcgc cctcgctcag gtccacgacc ttgacaatct
     5041 caatgagttt gttcaactgc ttggtaatct gctcgatcac gtcgtcggat ccgacggtaa
     5101 ccagcgtcat gcgggacaat gtgggatctt cggtagccgc caccgtcaat gattcgatgt
     5161 tgtagccacg ggatgaaaac aggcctgcta cccgcgacaa tgcacccgcc tcgttttcca
     5221 tcagaagaga aatgatgtgc cgcattttct ataccaggat catttcggag aggcctttgc
     5281 caccgggaac catcgggaaa acgttctcgg tctggtccgt tatgaaatcc atgaatacca
     5341 gccggtcttt gagcttgaac gcttctctca aagcgccttc gacgtcctcg ggtcgctcga
     5401 ttttcattcc gacgtggcca tagctctctg cgagtttgac aaaatcggga agcgcgtcca
     5461 tgtaggattc cgcgtagcga ttgccgtgga agaattcctg ccactgccga accattccca
     5521 tgtagcgatt gttcaaattg atgatcttga tggggagatt gtattggcgg caggtcgaca
     5581 actcctggat gcacatctgg atgcttgcct cgcccgtaat gcacgcgaca tcttcacctg
     5641 gattcgcgaa ctgaaccccc attgccgcag gaagcccgaa gcccatggtt cccaggcccc
     5701 ctgaattgat ccattgcttt ggcttatcga atttatagaa ttgagcggcc cacatctgat
     5761 gctggccgac atccgaagta acgaacgcgt ttcccccggt aacctcgtag agcttctcga
     5821 taacccattg cggctttata accgggcttg ccctgtcata tctcaggcaa tcgcgcgagc
     5881 gccatgactc gatttgcgtc caccatgcct gcaacgcggt ctgatccgtt ttttccttcc
     5941 gaactttaag ctgcctgaca agctcggcca gtacatcggg gacacttcca acgatgggaa
     6001 tgtcgacctt gacgcgcttc gagatggacg aggggtcgat gtcgatgtga ataatcctcc
     6061 gatcctcatt gaaaaaatgc ttcggattgc cgataacccg gtcgtcgaac cgggcgccga
     6121 tagccaccag cacgtcgcaa tgctgcatgg ccatattggc ttcgtaagtg ccatgcattc
     6181 ccagcattcc taaaaatttg ggatcggtcg cgggatatcc cccgagtccc atcagagtat
     6241 ttgtgcacgg aaaaccgagc attcttacaa gttcggtcag ttgcgccgcg gcgtcactca
     6301 ggataactcc gcctcccgtg tagatcatgg gacgcttggc ttcgaggatg agctgcacgg
     6361 ctttctttat ttgtcctgca tgtcccttca gtacagggtt ataagagcgc atcgcaacat
     6421 tttccggata cacaaaaggt gctttctgct ggcttacgtc tttgggtata tccaccagca
     6481 cgggtccagg gcgacctgtc gatgcaataa aaaatgcctt cttgatggtt gttgccaaat
     6541 cggccacgtt cttcacgagg aagttatgct tgacgcaggg gcgcgtaatt cctaccgtat
     6601 ccacctcctg aaacgcgtcg agaccgatcg cgtacgttgg tacctgaccg ctgatgacga
     6661 cgagcggaat cgaatccata taggcagtgg caatgcctgt taccgcattt gttacacccg
     6721 gcccggaggt gaccagagcc acgcctactt tattgcttga gcgtgcatac ccgtcggccg
     6781 cgtgcactgc tgcctgttcg tgccgcacaa ggacgtgttt caccttatcc tgtttgaaga
     6841 gttcatcata aatgaaaagt acggcgcctc cggggtagcc aaaaatatga tccacccctt
     6901 ccttttgcaa gcaacgtatc gttatttccg ctcccgtcaa ctccatcgtc atgcctggta
     6961 gaatcctgat atgtattaag tctcgcaaaa atgctgaatg caaacactga acagtaacgg
     7021 ctggataatg tttggtcaag ccggaaatag cgcaattagg ccggtcagct taatcgacca
     7081 ttataattcc tgataaaaaa tggttcgttc cttcaggcag aggagcagac tgtcaccctg
     7141 ggacgcggga ggtatcaggg acgagcgaga tcggggcgat caatctccag gtcgcagccg
     7201 atgaagccgg ggcaggatta gcgggccttt acaagcgcac ggtatatgca acaagattcg
     7261 catttgggcc tacccatgca ttttgatcag cgcttcatgc cttcggaaga agtttgctac
     7321 tcggtttttc ccaaaggtct ttcaaccggc ttgcggtagg ggcaagccgg ctttatgcaa
     7381 tccgcgtaaa gcgacagcga atgctcatgt atggcaaagc cgcgatcctt ggcaatcttg
     7441 ctttggcgct tttcgatctc cgcatcgtag aattcctcca cgcggccgca ttgcaggcaa
     7501 acgaggtgat cgtgatgggt accgcttgcc agctcgaaca ccgccttccc gccctcgaaa
     7561 tgatgccgct cgagcagccc ggcctgctcg aactgggtca acacgcgata aactgtggcc
     7621 agtccgatat cctctccttc attgatcagg gttctgtaga catcttccgc tgacaagtgc
     7681 cttaccgagc tgctttcgaa caggctcaat attctcagcc tgggcagcgt tgctttcagc
     7741 cccatggtct tcaggttgtt cgagttagtc atggtgatgt ccatccggtg ggtcgatttg
     7801 cggtatcata taacgttaag tctgctttgg aaaccgctcc gggatgcgcg cgcgaatact
     7861 tacgctggtt gctttgctgc tcgccggatg ttcttctgtt ccatctctgc catataaaat
     7921 cgaagtccag cagggtaatg tgattaccca ggaaatggtg gataagctaa agccggggat
     7981 gacccggtct caggttcgtt tcgtgcttgg ctctcccatg atcagcgatg catttcacga
     8041 taacaggtgg gattatgtct atcgcctgga gcaggcgggc aggctcattg agcagcgcaa
     8101 actcgttgtt tttttcgaag aggacaagct ggttcggctt ggagggagtt tttccccggt
     8161 tgcctttaac aaaccaccat ccataccttc tgcggagccg cccaagacag ccggggcttt
     8221 gaccttggag ccaggttccg tttctggggg agaacttcct gggccggtga cctccgcggt
     8281 gccgccccag ggaacaccgg ctcaaggcag tatcgctgcc gcaaccagcg agcagggtag
     8341 cgcagccgcc cgtgttcccg tgagaaaccc tgttccaagg caggttcctt cgcaggcaac
     8401 ggacatgggc gacgaagagg cggatcaac
//
LOCUS       DASSHJ010000202         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1426640,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000202 DASSHJ010000000
VERSION     DASSHJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426640"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFI43_07880"
     CDS             complement(<1..1096)
                     /locus_tag="VFI43_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7062083.1"
                     /translation="MNSIKSQIMVFAVLATLIPSLGLGLLSYRQNEMQTSENVTRQLH
                     ALTNYASREIELWRDRRAHEVNVTATSNAVIDTLSPAGRTHMRSPAKNPRALAHYLNS
                     VAEKLDTILELTVLDASGVTVASSARKPTSVPFPSNWPANAATEGLVVAPPQWDERFG
                     TASITIAIPVLSYDNLIMGAVVAVLDLGALQPHLKSPVKSPPGEVLLLDSSGRILVGS
                     QPGAPGQGHVDPLLLQRLAAQPGDSQVFQGVTHPEVLGLAEESKDLGVTVLAERDLAE
                     IHNAWADLRHGFLILVGFLILIVAAVAFKMGSSIVLPLRRLISAADRIAEGDLEVRLS
                     ATRNDELGHLTRVFNQMADRLRRSHAEIMAA"
     gene            complement(1348..>2512)
                     /locus_tag="VFI43_07885"
     CDS             complement(1348..>2512)
                     /locus_tag="VFI43_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HET7062084.1"
                     /translation="TPIPRTLAMSLEGLRDFSVIATAPQRRLAIKTFINRFSDGIIRE
                     ACLRELKRGGQIYFLHNEVSTIQTVHEKLVRLLPEARINVAHGQMRERELEHVMKDFY
                     QQRFNLLICTTIIETGIDVPTANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLT
                     PDEEALGAQARKRLEAIQSMEELGAGFYLAMHDLEIRGAGEVLGESQSGEMQEVGFNL
                     YASMLDTAVRSLKEGKEPDMQHPLGIATEINLHVPALLPDEYCGDIHERLILYKRMAS
                     CTDEDQLDDMQIELADRFGVLPDPVKALLECHRLRISAKPLGITRIDASGENIQIQFA
                     PNPPIKPANIVSLIQHDRNYQLSGPDRLKIQMQIPEVKERVRRIKSLIAELAA"
BASE COUNT          506 a          682 c          713 g          611 t
ORIGIN      
        1 ttgcggccat gatctcagca tgactccggc gcagtcgatc cgccatctgg ttaaacacac
       61 gcgtaagatg cccaagctcg tcatttcgcg ttgccgagag tcgcacttcc agatcccctt
      121 ccgcaatccg atccgctgcg ctaatgagcc gccgtaacgg caaaacaatg gaacttccca
      181 ttttgaaagc aacggcagcc actatgagta tcaaaaaccc gaccagaatc agaaacccgt
      241 ggcgcaaatc agcccatgcg ttatggattt ccgcaagatc gcgctccgcg aggacggtca
      301 cgccgagatc tttggattcc tcggccagtc cgagcacctc gggatgagtg acgccctgaa
      361 acacttgaga gtcgccaggc tgagcggcga gccgttgcag caatagcgga tccacgtgtc
      421 cctgaccagg cgcgccgggt tggctcccca ccaggattct gccacttgaa tccagcagca
      481 gcacttcgcc gggtggggat ttgacagggc ttttgaggtg gggttggagc gctccgagat
      541 caagcacggc gacaacagcg cccatgatga ggttatcata ggacaacacg gggatggcga
      601 tggtaattga agcggtaccg aatctttcgt cccactgcgg cggggcgacc acaaggcctt
      661 ctgtcgctgc gtttgcgggc cagttactcg ggaacggcac gctcgtcggc ttcctggcgc
      721 tgctggccac cgtcaccccg cttgcatcca gtactgtcaa ttccagtatc gtgtcgagct
      781 tttctgctac cgaattcagg taatgggcca atgccctcgg gttttttgca ggcgagcgca
      841 tatgggttct gcccgccggt gacaacgtgt cgatgaccgc gttcgatgtc gccgtgacgt
      901 tcacttcatg ggcgcgtctg tctctccaga gttcgatttc gcggctggca taattcgtca
      961 gcgcatgcag ttgccgagtg acattttcgc tggtctgcat ttcgttttgc cgatatgaca
     1021 gcaaaccaag cccgagcgat ggaataaggg tggcgagcac ggcgaatacc attatctggc
     1081 tttttatgct gttcatgcta accgtcagga gtaattgcgc gccggggatc gtccggtgga
     1141 tagtgccgga aaatggttgt tattcttgag ttgttcgatg atttaacgga aaaacgaaca
     1201 acagttgcgg ttcggcggca gcagcggcct gggtcccttc tggcacaccc atcatgtttt
     1261 gcgtgattca gcagcgtctt ctggagcgag cagtcttgcc catcctaccg gtatcttctt
     1321 tcaaccgaac gatgaataag agaggtttta agccgcgagc tcagcaatta ggctctttat
     1381 gcgcctgaca cgctctttca cctctgggat ctgcatctgt atttttaaac gatcaggtcc
     1441 cgaaagctgg taattacggt cgtgttgaat gagcgagacg atgttcgccg gctttatcgg
     1501 agggttcggt gcgaactgga tttgtatgtt ctctccgctc gcatcaattc gcgtgatacc
     1561 cagcggtttt gcggagatac gaaggcggtg gcattcgagc agcgctttga cgggatcggg
     1621 cagcactcca aaccggtcag ccagttcgat ctgcatatcg tccagttgat cttcgtccgt
     1681 acagcttgcc atccgcttat acagtatcag gcgctcgtgt atatccccgc agtattcgtc
     1741 cgggagcagc gcgggtacat gcaagttgat ttcggttgcg attcccaggg gatgttgcat
     1801 gtcgggctct ttcccctcct tcagggagcg gacggcagta tcgagcatgc tcgcgtataa
     1861 gttgaaaccc acttcctgca tttcaccgct ttgtgattcc ccaagaactt cgcctgcccc
     1921 gcgaatctcc aggtcgtgca tggcaagata gaagcccgct cccagttcct ccatcgactg
     1981 tatcgcttcc agccgctttc tggcctgcgc tcccaaggct tcctcgtcgg gagtgagcag
     2041 gtaggcatac gcttgatggt gcgagcgtcc gacgcggccg cgcaattgat gcaattgcgc
     2101 aaggccgaat ttatccgcac gattgataat gatggtgtta gcggtaggga catcgatacc
     2161 ggtttcgata atggttgtgc agatcaggag attgaagcgc tgctggtaga agtctttcat
     2221 tacgtgttcg agttcgcgct cgcgcatttg cccgtgagcg acattgatgc gggcctccgg
     2281 cagcaggcgg accagctttt cgtggacagt ttgaatcgta ctgacctcgt tgtgcaggaa
     2341 atagatctga ccaccgcgtt tcaattccct gaggcacgct tcacgtatga tcccgtctga
     2401 aaagcggttt atgaaggttt ttatcgcgag gcggcgctgt ggcgctgtgg caatgaccga
     2461 aaaatcccgc aagccttcca gggacattgc gagtgttcgt ggtatgggtg tg
//
LOCUS       DASSHJ010000203        10393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1357588,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000203 DASSHJ010000000
VERSION     DASSHJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357588"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFI43_07890"
     CDS             complement(<1..367)
                     /locus_tag="VFI43_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HET7062085.1"
                     /translation="MKKEYWLERWEREDTGFHQEEINPYLREYWHELHLAKNSEVFVP
                     LCGKSRDMLWLRDQGSSVLGVELSAHAAEAFFLETGQTPRHSPGESFDRWEANGITIL
                     CGNFFNVTKDDLRNVNGVYD"
     gene            504..1370
                     /locus_tag="VFI43_07895"
     CDS             504..1370
                     /locus_tag="VFI43_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062086.1"
                     /translation="MDFPPHNSKLTIFALSSGRSGTGFLADFFAKNVYQCYSVHEPYL
                     VPGNPVLFGRPIGWNTAHNDKRLLPILERKCGFIASCKDPVYFESNHAFLKVFNRHAG
                     ALLHDVGFVHLVRHPAKVAKSEMLRERLIHRAHLPFMHYISDTGERCLRWALTGKEEI
                     FRYYAGNILSRFQFYFLQWIEIEYRAMQLLKENRWQDRVFFIDVDSQLQDETVLRQML
                     DFFGLRYKAGFNMNLRRNRIPFMRPTIVSPQDEMEIQAVMQNLPCKYKAMLMGEPYSK
                     CSAWEALRRYCV"
     gene            complement(1522..1959)
                     /locus_tag="VFI43_07900"
     CDS             complement(1522..1959)
                     /locus_tag="VFI43_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7062087.1"
                     /translation="MINNEKAYEGSCFCGAVQFIVRGEPAGMGYCHCESCRRWSAGPV
                     NAFTLWKPEAIQITKGVENIGTYNKTPRNFRKWCKTCGGHVFTEHPHWGLTDVYAAGI
                     PSFPYHPAIHVNYQETVLPIKDDLPKMKDLPKEMGGSGDIITE"
     gene            2433..4589
                     /gene="ovoA"
                     /locus_tag="VFI43_07905"
     CDS             2433..4589
                     /gene="ovoA"
                     /locus_tag="VFI43_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HET7062088.1"
                     /translation="MPVSADSSQDSEVRDTHKPLFSRTPVLVGDNVEAKREEIRRYFH
                     STLDRYEALFETLEGDEAFYKKPISLRHPLIFYLGHTATFFTNKLVLAGLIEERINPK
                     LESMFAVGVDEMSWDDLDNTHYDWPFVDDVYAYRRTVRNVVDKLITDTPLTLPITWES
                     PWWTIMMGIEHERIHLETSSVLIRQHELRHVRPQPVWEPCRKTGPAPRNKLIDVPEGQ
                     VNLGKKKSDQTYGWDNEYGTHIADVPAFQAGKYLVSNQEFLEFVEAKGYATERFWEAE
                     GLAWRNHTGAQYPTFWINDSGEWRLRLMTEEISMPWDWPAEVNYHEAKAFCNWKAEMT
                     GQPFRLPTEDEWYRLYDAAGLAEIASTEQAAGNIHLDYWASSCPVNQFSQGEFFDVMG
                     NVWQWTETPIYPFEGFDIHPHYDDFTTPTYDGRHNLIKGGSWISCGNESLKSSRYAFR
                     RHFFQHAGFRYVVSDAPAIQNDSHYETDKLLSEYAEFHYGESYFGVSNFPRALAELAI
                     AATDGKPRRKALDLGCASGRATFELAREFEEVTGIDFSARFIGQGVQLATKGTLRYTL
                     TDEGELVFYKERTLSGLGLEAVKHKVTFYQGDACNLKPLFGGYDLILAANLIDRLYDP
                     AKLLTTIHERLNMGGVLLIASPYTWLEDHTKRDAWIGGFKRDGESFGTLDGLREMLGA
                     RFKLIRGPQEVPFVIRETRRKFQHTLSEATIWERIA"
     gene            4586..5818
                     /locus_tag="VFI43_07910"
     CDS             4586..5818
                     /locus_tag="VFI43_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7062089.1"
                     /translation="MSVPGSRDFDSIIDRSGTSSLKYDGRRSMFGAPDVIPLWVADMD
                     FAAPPAVTRALGERALHPIYGYTLYPDSLYESLMAWLKRRHGWDVHRDWIMMCPGVVP
                     SLHTAVAAFAEPGASVIVQPPVYFPFFSSVTTTGRKLTLNPLVLRNDAYTIDFDHLEH
                     CAEEGARLLLLCSPHNPVGRVWSRRELNQILQIARKYGLILFSDEIHADLVYPGNKHH
                     VISMLAQAAGTDVPIITAVAPSKTFNIPGLNLSVIIVPDPECREKLSRTFEAMHVSAS
                     NPFSIVAFEAAYREGERWLEDLLVYLAQTRDLVHRYLAAHIPQIRLIEPEGTYLLWLD
                     CRVLKSELGMDDMQLRHFLVHQAGVGMSPGTLFGEAGSGFMRMNIGTPRKTIETALKN
                     IRNAIKLNRANVGGVHQA"
     gene            5820..6719
                     /locus_tag="VFI43_07915"
     CDS             5820..6719
                     /locus_tag="VFI43_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7062090.1"
                     /translation="MDKAGGARIGWASLLGVICIWSGFILITRHSTTGALTSWDIGML
                     RFGVGALVSVCFLPFIALPAPRVIALFSLFGGVGYALVVYAAFHMSPAAHASILLPGT
                     LPFATAMIAWLWLGQKPLPLHLAALASVFAGVMMTAADSLSQGPRLTLTQITGDVLFF
                     CGSLSWGVFTLLLGRYSVQPLAAAVTTTLGSAMLYLPIWWLFLPTGLMQASMPEIVMQ
                     AVYQGVLVVFISMMLYSVAIDRLGPHTVALQITFVPVISSLLAVPILGETISVLEWGG
                     LVAVTAGALLGARASGRAFNQYG"
     gene            6842..9178
                     /locus_tag="VFI43_07920"
     CDS             6842..9178
                     /locus_tag="VFI43_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7062091.1"
                     /translation="MQDPKLFLVPPTIPLAGTPPIPNEWTVQVESRLLPQEQVLAWLQ
                     TDLSSRLYYAPGLVIVTDKRLLSTAESEGVWQDWFYRPGLKLARSDHSGIGSLELFDE
                     ASLLAKWRYTLGNDAAASRLIESFTRQLSYQLTAALPLAQEQHVCSRCQRVLDAPLPP
                     GQDECAACVREASTPPSTWTLFRLWRFAKPYKMRLLAGFLLTVGSTGATLVPPYLTMP
                     LMDNVLIPYQNGKPIDVSLVTFYLTGLLGAALLAWVLGWSRTYILALVSERIGADLRT
                     ATYEHLLKLSHAYFAGKRTGDLMARIGSETDRINVFISLHLLDFATDVLMITMTVVIL
                     VSIHPSLALVTLLPLPIIAWLIHLVRDRLRTGFEKVDRVWAEVTSVLADTIPGMRVVK
                     AFAQEKREASRFRAANDRNLEVNDRVNKVWSLFTPTVTLLTEIGLLVVWVFGIWQLSM
                     NEVTVGVLTAFLAYISRFYLRLDSMSRIVSFTQKAAAGTKRIFDILDHVSSVPEPARP
                     VHLPVVKGHIELQNVGFRYGTRTVLRNVNINIKPGEMVGLVGHSGSGKSTLVNLICRF
                     YDVTEGAILLDGVDIRSLPVVEYRKHIGLVLQEPFLFFGTIAENIAYGKPDAGLDLIV
                     DAARAAHAHDFILRLPHGYDSLVGERGQALSGGERQRISIARALLIDPRILILDEATS
                     SVDTTTEKEIQKALENLVRGRTTIAIAHRLSTLRDADRLIVLDRGNVVETGSHLELMA
                     RGGHYYQLYQAQARNVDVEDSLLRAPISEHKAGEGREQ"
     gene            9175..9645
                     /locus_tag="VFI43_07925"
     CDS             9175..9645
                     /locus_tag="VFI43_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HET7062092.1"
                     /translation="MSASGFTLQRDKFGKLTFTGADGRVHEGVVPVRAFPITSVGWGL
                     ALVDRHGHEVAWMTNLGDLPEESRKLIEAELDSREFVPEITRIVSVSSFATPSLWQIE
                     TDRGDTTLMLKGEEDIRRLSPSALLVADSHGVHFLIRDRFTLDRQSRKLLDRFL"
     gene            10083..>10393
                     /locus_tag="VFI43_07930"
     CDS             10083..>10393
                     /locus_tag="VFI43_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062093.1"
                     /translation="MLPGTDTNAGFANNDIINNRDISFLEIRHLNGPNIWTLRPVLET
                     IIDIGDLEDCPSDTIPGFYERLSSWLPSLIEHRCSYGERGGFLRRLKEGTWPGHILEH
                     VT"
BASE COUNT         2240 a         2794 c         2793 g         2566 t
ORIGIN      
        1 gatcgtagac tccatttaca tttcgcagat catcttttgt tacattgaaa aagttcccgc
       61 agagtatggt tatcccattc gcttcccagc gatcgaacga ttcgccgggg ctgtgccgtg
      121 gcgtttgacc agtttccagg aaaaatgctt ccgctgcgtg cgcacttaat tccactccca
      181 gtactgaact cccctgatcg cgaagccata gcatgtcacg gcttttgcca cacagtggca
      241 cgaacacttc actatttttc gccaggtgca gttcgtgcca gtattcgcgc aaataaggat
      301 tgatttcttc ctgatggaaa ccggtatcct ctcgttccca acgttccagc caatattctt
      361 ttttcattcg ttatccaagg gcatgtttcg ataaaaagtg acggatggta aagctgttcg
      421 ttgtcctgcc tttggcgcac gcacattagg ggataatacc tgaccaagct agcattatct
      481 ggacattcca gatcgcctga cccatggatt ttcccccgca caactccaag ctgacaattt
      541 ttgcactttc cagcggccga tcgggcacag gttttctcgc tgactttttc gccaagaatg
      601 tgtaccaatg ttacagcgtc cacgaacctt atcttgttcc cggaaatcct gttttattcg
      661 gtaggcccat cggctggaat actgcgcata atgataagag gctgctgccg atcctggaaa
      721 ggaaatgcgg tttcattgcc agctgtaaag acccggtata ttttgagtcc aaccacgcct
      781 tcctgaaagt ctttaaccgt catgcagggg cattgctgca tgacgtcggt tttgttcatc
      841 ttgtgcgtca tccagccaag gttgccaaaa gcgaaatgct gcgtgaacgc ttgatccata
      901 gggcccacct gccttttatg cattacatta gcgatacggg tgagcgttgc ttgcgctggg
      961 cacttacggg aaaggaggag atcttccggt attacgcggg aaatatctta agccggttcc
     1021 agttctattt ccttcaatgg atagaaatcg aataccgggc aatgcagctg cttaaagaga
     1081 atcgctggca ggatcgcgta ttctttatag atgtggacag ccaattacaa gatgaaacag
     1141 tattacggca aatgctcgac tttttcggct tgcgttataa ggccggtttt aacatgaatt
     1201 tacgcaggaa cagaattcca ttcatgaggc ccaccatcgt atctccccag gatgagatgg
     1261 aaatacaggc cgtcatgcaa aacctgcctt gtaaatataa ggcaatgttg atgggagaac
     1321 cttactcgaa gtgttccgct tgggaggccc tccgccggta ttgcgtgtag aaatccaggt
     1381 cagttatcgc tgcatagggg aactgccatg accgattccg cggtggaatg cgcggcgaga
     1441 cagggaagca cgtgcgaagc agggtgagtg gttttcggcg aagagactct gctggtattg
     1501 aacctcgctt ggttcatcct gttattccgt tataatgtca cccgacccac ccatttcctt
     1561 cggtaggtct ttcatcttcg gcagatcgtc cttgatgggt agcaccgtct cctggtaatt
     1621 tacgtgaatt gcgggatggt acggaaacga gggaatgcca gcggcgtaaa catccgtcag
     1681 tccccaatgc ggatgctcgg taaaaacgtg gcccccgcac gttttgcacc acttccggaa
     1741 atttcgaggc gtcttgttat acgtgccgat attttccact ccctttgtaa tctgtatcgc
     1801 ttccggtttc cataaagtga aagcattgac gggtcctgcc gaccagcggc ggcaagactc
     1861 gcagtggcaa tatcccattc cagccggctc acctcttacg ataaactgaa ctgccccgca
     1921 aaaacagctg ccttcataag ccttttcgtt gttaatcatg attcatctcc gacgatgcgc
     1981 gacgagtgac gacttgaaaa gataagccgg attgtattca gcatgaagtt ccatctggct
     2041 caatgagggg gcttccccag tcagtcgctc ccgttcaatc cttatgtaac ttctgcctga
     2101 tttatcagaa gaaagcgaag gaagcgcgta gcagatcggg tagcaagggt accaacaggg
     2161 gaactcatcc ccgccaccag actcgaaggc gacattaccg ctctttcacc cagtttgatc
     2221 ctcatcaaga ctatcgagca aggcgcgcgt cctgatctcg catgactgca caattatcga
     2281 gctataatct ccccagtttt tattatcgca cttgattatc agcttgagcc agactcggct
     2341 tgatcaattc attcctttat tttacataat ttgttaaagg ccaattctaa ccgaggtcaa
     2401 atatgaattt gaacgatgct cgaaaagtca taatgcctgt gtcagcggat tcttcacaag
     2461 attcagaggt gagagatacg cataagcctt tgttttcgcg gactcctgtt cttgtgggag
     2521 ataatgtcga ggcaaaacgc gaggaaatac ggcgctattt tcactccacg ctcgatcgct
     2581 acgaggcact tttcgaaacc ttggaggggg acgaggcttt ttacaagaaa ccgatctcgt
     2641 taagacatcc tctcattttc tacctgggcc acaccgccac cttttttacc aacaaactcg
     2701 ttcttgccgg tttgatagag gagcggatca atccgaaatt ggaatcgatg ttcgcggtcg
     2761 gcgtggacga aatgagctgg gacgacttgg ataatacgca ttacgattgg ccctttgtag
     2821 atgacgtcta cgcctaccgt cgaacggttc gaaacgtggt tgacaagctc atcacggata
     2881 ctccgctgac attgccgatc acttgggaaa gcccttggtg gaccatcatg atgggtatcg
     2941 agcacgagcg aattcatctc gaaacatcgt cagtcctgat caggcagcat gagcttcggc
     3001 atgtcaggcc tcaaccagtc tgggaacctt gtcgcaagac cggtcccgca cctcggaata
     3061 aactgattga cgtacctgaa ggccaggtga atctgggcaa aaagaaaagc gatcagacgt
     3121 atggatggga caatgaatac ggtacccaca tcgcggacgt gcccgctttt caagcgggta
     3181 aatatctggt gagtaaccag gagtttttgg agtttgtcga agcaaaaggg tatgccaccg
     3241 aaaggttttg ggaggccgag ggtttggctt ggcgcaatca tacgggggcg caatatccca
     3301 ctttctggat aaatgacagc ggggaatggc ggttgcgcct gatgaccgag gagatttcga
     3361 tgccgtggga ttggccggca gaggtgaatt accatgaggc aaaggctttt tgcaactgga
     3421 aggctgagat gacgggccaa ccgttcaggc tgccgaccga ggacgagtgg tatcggctat
     3481 acgatgctgc tggattggct gaaatagcga gcaccgagca agcagcgggc aacatccatc
     3541 ttgattactg ggcctccagt tgcccagtga atcaattttc acagggtgag ttcttcgatg
     3601 tgatggggaa tgtatggcag tggactgaaa cccctatcta tcctttcgag ggtttcgata
     3661 ttcaccccca ttatgatgac tttacgacgc cgacctacga cggcaggcac aatctaatca
     3721 agggcggctc ctggatttcg tgcggaaatg agtcgctgaa gagcagccgc tatgctttcc
     3781 gccggcattt tttccagcac gccggatttc gctacgtagt gagcgacgcg cccgcgatcc
     3841 agaacgactc ccattacgaa actgacaagc tgctctcgga atatgcggaa tttcattatg
     3901 gcgagagcta ttttggcgtt tcaaactttc caagagcgtt ggcagaactg gcgattgcgg
     3961 caacggatgg caagccgcgc cggaaagccc tcgaccttgg gtgcgcctcg gggcgtgcaa
     4021 cttttgaact ggcgcgcgag ttcgaggagg taacaggaat tgatttctcc gcccgtttta
     4081 tcgggcaggg tgttcaactc gcaacgaagg gaacgttgcg gtatacgctg accgatgaag
     4141 gagaactcgt cttttacaaa gagcggacgc tttcgggcct cggcctcgag gcggtcaagc
     4201 ataaggtcac gttctaccaa ggcgacgcct gcaacctcaa acctctgttc ggtggatacg
     4261 acctcatcct tgccgccaac ctgatagacc ggctttacga ccccgcgaaa ctgctgacga
     4321 ccattcacga acggctcaat atgggaggtg tgttgctaat cgcttcccct tatacctggc
     4381 tggaggatca tacgaagcgc gacgcatgga tcggaggctt caagcgggat ggggaaagtt
     4441 tcggaacgct tgatggtctc agggagatgc tgggcgctcg tttcaaactt atccggggtc
     4501 ctcaggaagt cccttttgtt atccgcgaaa cccggcgcaa gtttcagcat acgctctcag
     4561 aggcgacaat ctgggagcgg atcgcttgag cgtccccggc tcccgtgatt tcgacagcat
     4621 aatcgatcgc tccggtactt cgagccttaa atacgatggg cgccggagca tgttcggcgc
     4681 tcccgatgtc attccgctgt gggtggcgga tatggacttc gccgcccccc cggccgttac
     4741 ccgggcgctt ggagaaaggg ctctccaccc gatatacggg tatacgctct atccggacag
     4801 cttgtacgaa tcattgatgg cgtggctcaa gcgccgccac ggctgggacg tccatcggga
     4861 ctggatcatg atgtgtccgg gcgtagttcc atcgcttcat accgccgtag cagcgttcgc
     4921 ggaacccggc gcgtccgtca ttgtgcagcc acccgtatac tttccttttt tttcgtctgt
     4981 caccaccacg ggcaggaagc tgacactcaa tcccctggtg ttgcgaaatg acgcgtacac
     5041 cattgatttc gatcacctcg agcattgcgc cgaggagggc gcgcgcctgt tgctgctatg
     5101 ttctccgcac aatcccgttg gacgtgtatg gagcagacgt gaactgaacc agatcctgca
     5161 gatcgccagg aaatacggac tcatcctttt ttctgatgaa attcacgcgg acttggtcta
     5221 tcccggcaac aagcaccacg tgatttcaat gcttgcgcaa gcggccggta ccgatgttcc
     5281 catcattaca gctgtagcgc ccagcaagac gttcaatatt ccgggcctca atctttccgt
     5341 tattatcgtg cccgatccag agtgcaggga aaagctttcc cggacgttcg aggccatgca
     5401 cgtgagcgcg tccaacccat tcagcattgt cgccttcgag gccgcttacc gggaagggga
     5461 acgatggctg gaagacctgc tcgtttatct ggcgcagact cgggatctgg tgcaccgtta
     5521 cctggcggcg catattccgc aaatccggct tatcgagccg gaaggaactt acctgctctg
     5581 gctcgattgc cgtgtgctaa agagtgagct ggggatggac gatatgcagc tcaggcattt
     5641 tttggtgcat caggcggggg ttggcatgag ccctggcacg ctatttggtg aggcaggcag
     5701 cggcttcatg cggatgaata taggtacgcc gcgtaaaaca attgaaactg cgctcaaaaa
     5761 tatacggaat gcgatcaaac tgaaccgtgc gaacgtaggc ggcgtccatc aggcctgagg
     5821 tggataaagc cgggggtgcc cgtatcggtt gggcctcgct cctcggcgta atttgcatct
     5881 ggagcgggtt tatcctgatt acccggcaca gcacgacagg cgcgcttacc agttgggaca
     5941 tcggtatgct gcgtttcggt gttggcgcgc tcgtctcagt ttgttttctg ccctttattg
     6001 cattgccggc gccgagagtg attgcgctat tttcactttt tggcggcgtt ggctacgcgc
     6061 ttgtggtgta cgctgcattt cacatgtctc cggcagcgca tgcgtcgatt cttttgcccg
     6121 ggacgttgcc cttcgctacc gcgatgattg cgtggctatg gctcggccag aaacccctgc
     6181 ctctccatct ggctgccctt gccagtgttt ttgccggagt catgatgacc gcagccgact
     6241 ccctgtccca agggcccagg ctgacgctca cgcaaattac cggagatgtt ctattttttt
     6301 gcggttcatt atcctggggc gtattcacgc ttctgctcgg acgctattcc gtgcagcccc
     6361 tcgccgctgc ggtgaccact actttaggga gcgccatgct ttacctgcca atctggtggc
     6421 tattcctgcc caccgggctc atgcaggctt ccatgccgga aatcgttatg caggcggtat
     6481 atcaaggtgt cctcgtggta tttatctcga tgatgctcta cagcgtggcg atcgatcggc
     6541 tcggccccca tacggtggcg ctacagataa cgttcgttcc cgttatctca tctctcctgg
     6601 cagtgccgat tctcggcgaa acaatatctg tgctcgaatg gggcgggctg gttgctgtga
     6661 ctgcaggcgc attactgggt gcaagagcat cgggtagagc gttcaatcag tacgggtgac
     6721 ctcgctttcg gataagggca taatgtccgg acggggcgcc gctgagtctc aggctgccgc
     6781 attgctcggc tagctatggg cctcgattcc tgcgaaagcc ttttgatttc tggatgtagc
     6841 gatgcaagac cccaagcttt tcctggttcc tccaacgatt ccgttagccg gcacgccgcc
     6901 catccctaat gaatggacag tgcaagtcga atcgcggctt ctcccccagg aacaggtatt
     6961 ggcctggcta caaacagatc tgagttcccg gctttattac gcgcccggcc ttgtcatcgt
     7021 tacagacaag cgcttgttga gtaccgcaga aagtgaaggg gtatggcagg actggtttta
     7081 ccgtcccgga ttgaagctgg cacgctcgga tcattccggg atcggaagcc tggaactttt
     7141 tgatgaggca tcattgcttg ccaagtggcg ctacacgctc ggaaacgatg ccgcggctag
     7201 caggctgatc gaatctttta ctcggcaact cagctaccag cttacggctg cattgccgct
     7261 tgcgcaagaa cagcacgtat gctcgagatg ccaacgcgtc ctcgatgctc cgctgccgcc
     7321 tggccaggac gagtgtgccg catgcgtcag ggaagcctct actcctcctt caacctggac
     7381 attatttcgc ctgtggcgct ttgcaaagcc ctacaagatg cgcttgcttg ccggtttcct
     7441 gctgaccgta ggcagcacgg gtgcgacgct ggttccgcct tacctgacga tgcctctgat
     7501 ggacaatgtg ctcattcctt accagaacgg caagccgatc gacgtcagtc tcgtgacgtt
     7561 ttatctcacc gggctgctcg gggcggcgct tcttgcctgg gtgctgggat ggtcgcgaac
     7621 gtatattctg gccctggttt cagagcgcat cggcgccgac ctgagaaccg ccacctacga
     7681 gcatcttctc aagctgtcac acgcttactt tgctgggaag cgcacgggcg accttatggc
     7741 tcgcataggc tctgaaacgg atcgaatcaa cgtcttcatc tctcttcatt tgctcgactt
     7801 cgccacggat gtgctgatga tcaccatgac cgtggtcatc ctcgtctcga tccatccgtc
     7861 gcttgcactc gtgacgctgc ttccgctacc gattatcgcc tggctgattc acctggtccg
     7921 ggatcgcctg cgtacggggt ttgaaaaagt ggaccgcgtt tgggcagagg tcaccagtgt
     7981 tcttgcggac acgattcctg gcatgcgggt agtcaaggca tttgcgcagg aaaaacgcga
     8041 ggcctcgcgc tttcgggcgg caaacgatcg taatctggaa gtgaacgacc gggttaacaa
     8101 ggtctggtca ttattcacgc caaccgttac cctgctcaca gagatcgggt tgctggtggt
     8161 ctgggttttc gggatctggc agctatccat gaatgaggtc acagtaggcg tgctgactgc
     8221 cttcctcgcc tacatcagca ggttttacct tcggctggat tccatgagcc gcattgtttc
     8281 ctttacacaa aaggctgcgg cgggaacgaa gcggattttc gatattctcg accacgtgtc
     8341 aagtgttccc gaacctgcac gtcccgtgca tctgcctgtg gtcaagggac atatcgagct
     8401 gcagaatgtc gggttcaggt atgggacccg taccgtgctc cggaacgtaa acatcaatat
     8461 aaagcccggt gaaatggtcg ggcttgtagg ccatagcggt tccggcaaga gtacgctcgt
     8521 caacctgatc tgtcgctttt acgatgtcac ggagggggca attctgctcg atggggtcga
     8581 tatccgctcg ctgcccgtgg tggaataccg gaagcacatc ggcctcgttc tccaggagcc
     8641 gttcctgttt tttgggacaa tcgccgaaaa catcgcgtac gggaaacctg atgccggact
     8701 cgacctaata gtcgacgctg cccgggccgc tcatgcccat gacttcattc tgcgtcttcc
     8761 ccatggatac gattcgctgg tcggcgaacg cgggcaggct ctctcgggag gcgagcgcca
     8821 gcgaatctcg atcgcgcgtg cgctactgat cgatccccgc atcctgattc ttgacgaagc
     8881 cacctcctcg gtggacacaa cgactgaaaa agaaattcag aaagcactcg aaaatctcgt
     8941 tcgcgggcgc accactattg ccattgctca tcgtttatcc acccttcgcg atgccgatcg
     9001 attgatcgtg ctggaccgag gaaacgtggt cgagacgggc agtcatctcg aacttatggc
     9061 tcgtgggggt cattattatc aactgtatca ggcccaggca cgtaacgttg atgtcgaaga
     9121 cagcctgctg cgcgcgccga tttcagagca taaagccggg gaggggcgcg aacaatgagt
     9181 gcttccggat tcactttgca acgcgacaaa ttcggcaagc ttacgtttac cggagccgac
     9241 gggcgggtac acgagggggt ggtgcccgtc cgcgctttcc cgattacttc agtgggttgg
     9301 ggattggcat tggtcgatcg acacggccat gaggttgcgt ggatgacgaa cctcggcgat
     9361 ttgccggagg aatcccgcaa gctgatcgag gccgaactcg atagccggga attcgttcct
     9421 gaaatcacgc gtattgtaag cgtcagcagt tttgccaccc caagcctctg gcaaatcgaa
     9481 acggatcgtg gcgatacgac gctgatgctc aagggcgaag aggatatccg gcgcctgtct
     9541 ccctcggcgc ttcttgtcgc tgacagtcat ggcgtacatt tccttatccg ggatcgcttt
     9601 accctcgatc gtcagagccg gaagcttctc gatcgttttc tctgactcaa ttcttgcccg
     9661 tcagtgataa ggcggaggcg aatgtctgtc gtccgcttcc cgccacgtta ccgcatctgc
     9721 tcatgtcgcc gggttgcgac atgaaataat attgctatat caagttctta tcccaaatac
     9781 tacccatttc tgtcgaataa tggcgacatc tccctccctc cttctcttcg gctatgtcac
     9841 ataagggatt gttagatcaa gaatttttaa atggcacgag aagtgcttgc gtctgcttgg
     9901 cggggcaagt cgagccgatt ccctcagttc gtatgaaaaa aacgagcagg agcgccataa
     9961 gccacgcgcg ttctttgatt tgttgagctc agacagccat ttcccttaaa tttatacacg
    10021 aggttgtaaa ccgatttaat gtattcgcgt tagttcaatt tccatccacc cctgcgactt
    10081 ttatgctgcc tggcaccgac acaaacgctg gctttgcgaa taacgatatc atcaacaaca
    10141 gggacatctc atttctcgaa atccggcatc tcaacggccc taacatctgg acacttcggc
    10201 cggtactcga gaccatcatc gacattgggg atctggaaga ttgcccctca gataccattc
    10261 caggttttta tgaacgtctt tcttcctggc ttccgtcgct catagaacat cggtgcagtt
    10321 acggggagcg cggcgggttc ctgcgccgtc tcaaggaagg gacgtggccg gggcacattc
    10381 tcgagcacgt tac
//
LOCUS       DASSHJ010000204        13800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1446269,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000204 DASSHJ010000000
VERSION     DASSHJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446269"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFI43_07935"
     CDS             complement(<1..195)
                     /locus_tag="VFI43_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HET7062094.1"
                     /translation="MPRRHIKEDSEKHDRWLISYSDFITLLLALFVVLYAISQVNEGK
                     YRMVANSLGSAFGSAPNRSVV"
     gene            complement(222..962)
                     /locus_tag="VFI43_07940"
     CDS             complement(222..962)
                     /locus_tag="VFI43_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HET7062095.1"
                     /translation="MDFSSITGILIALIAVLGGQALEGGSVASLIQFTSLGVVLGGTL
                     GAVLLQSPIRQFLLGMKMSRWVLIPPLLDEAGLVHHIGSWSSISRKDGLLALDTHIEG
                     IDDPFIRKGLQLLVDGIEPAVLKQILEVEIESFDDLHRQGARVWEAAGGYAPTIGILG
                     AVMGLIQVMENLSDPAGLGAGIAVAFVATLYGVGLANLIFLPVANKLKALVSRQVLMR
                     EILVDGLVGIARGENPRLITGRLNGYLV"
     gene            complement(944..1672)
                     /locus_tag="VFI43_07945"
     CDS             complement(944..1672)
                     /locus_tag="VFI43_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HET7062096.1"
                     /translation="MYTSAGIFDKQQHLTRFAPLVKRIAQHLIAKLPVSVEIDDLIQV
                     GMMGLMEALDRYEQMPGAQFATYAQQRIRGAMLDELRHMDWLPRSARKNMREIEAAIS
                     VLQQRQYRSPTEVEIAAELGVSVDTYHEMLFEARGVQLVHYEDFGENEEDDYLERHCA
                     DNAADPLVGLLDGDLRQSLIAAINALPEREKTLMGLYYEQELNFKEIGAVLGVSESRV
                     CQIHSQAIARLRARLKECAWTSAA"
     gene            complement(1677..2573)
                     /locus_tag="VFI43_07950"
     CDS             complement(1677..2573)
                     /locus_tag="VFI43_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7062097.1"
                     /translation="MTDTGHDQAEGLRRLLMQDSTRIVSVVSGGAGAGKTTSIINLAA
                     FLAASGKNVLAIDENACTSNICATLGLSAHRDLLDVIRRDKTLAQVLISAPLGFQILP
                     AGRGMRVLDKLNTGDQVHLIGCFAQIAQPMDIVLIDTAPGRSSRLLSLDFAGHELLVV
                     LAPEPASITSAYSLIKHVHGYHADKQRFHILINKSSMEREAGTVFENMARAADQYLGL
                     SLDFTGYIPTDGKLSLGLPVAEAFPDTPTAAAFREAAESLGKWSCREGESRMLERFIQ
                     RLLQNSRARRSERAQAVISGLR"
     gene            complement(2566..4254)
                     /gene="flhF"
                     /locus_tag="VFI43_07955"
     CDS             complement(2566..4254)
                     /gene="flhF"
                     /locus_tag="VFI43_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HET7062098.1"
                     /translation="MSIQKFFARTTSEALRKVRDVLGPDAIILSNRDIDGGIEILAAH
                     HDHISSLIPSHLSSAPHEAAGFEDLNPRAFRHAPGILSDKESRPRRDESRRDESRNGT
                     QTVSFSSWKDSLKRVELPTPPENYPSRENAVSSGKMEKKHRAPLPDFPIQHPKTSAGD
                     STGSGLREPAQPDEPLNDRPSQKRRNPPLSQFESSPLEIDNGRKKRSSSLSRTDAIIS
                     SESAAKSREAPVFDTHRLAEEVSASVIREINSMRSALEQQLALLNRSGEAHGAPVRRN
                     LVSRLLDAGFSARFAHRLLDRSAHISDMQNDEKQAMAHVHRLLAENLETVESEDEILE
                     KGGIYALVGPTGVGKTTTTAKLAARCVVRHGADKLALLTTDGYRIGGHEQLRIYGKIL
                     GVPVHTVRDAQDLAVILSELRGKHMVLIDTVGMSQRDEMVAEQVAMFTECDEEISRIL
                     LLNASSSLQTLDEVAGAYRGNGLAGAIITKIDEAVVSGGALEAAIRHRLPLYYVSTGQ
                     RVPEDLQLADRGKLIEAALEISPAFADSLPPDLFLPMVGRVTRSIGNAAGVPLD"
     gene            complement(4251..6353)
                     /gene="flhA"
                     /locus_tag="VFI43_07960"
     CDS             complement(4251..6353)
                     /gene="flhA"
                     /locus_tag="VFI43_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HET7062099.1"
                     /translation="MNSIAKLANVFGFNDAKDANRIKAGSFASPLLIFLILGMMVLPL
                     PAFLLDIMFTFNIALAIMVLLVSIHTHKPLDFAVFPTVLLLSTLLRLSLNVASTRIVL
                     MEGHSGPDAAGKVIEAFGHYLVGGNYTVGIVVFIILVAINFIVITKGAGRIAEVSARF
                     TLDAMPGKQMAIDADLNAGLIGEEEAGRRRTTIAQEAEFYGSMDGASKFVRGDAIAGI
                     LILFINVIGGLVIGVLQHDLDLSTAARNYTLLAIGDGLVAQIPALIISTAAGLVVSRV
                     GTDEDIGTQLASQLFSRPRVLALTAAIVGFMGLIPGMPHFAFLLLAGALAGGAYSMIR
                     RQATPAIKAPAAPNVQPEVKDASWDDVVPVDILGLEVGYRLIPLVDKTQDGELLRRVK
                     GVRKKFAQEIGFLPPPVHIRDNLDLRPGGYRITLKGVAIGSGEVSVGHYLAINPGQVS
                     GNLTGQATSDPAFGLPATWIDASQREHAQITGYTVVDAGTVVATHLNHLIHGHSAELL
                     GRTEVQQLLDRLATESPKLTEDLTPKFLPLASVQKVLQNLLEENVHVRDLRTIVETLA
                     EHAPRVQDPHELTALVRIALGRAIVQQIWPQSTELQVMALDSGLERLLTQALQSSPQG
                     AGVEPGLAETLLREASGGSKRLEQLGLTPVLLVAAPLRALLARFLRRALPQLKIISHA
                     ELPDSASIKVTSILGART"
     gene            complement(6386..7537)
                     /gene="flhB"
                     /locus_tag="VFI43_07965"
     CDS             complement(6386..7537)
                     /gene="flhB"
                     /locus_tag="VFI43_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HET7062100.1"
                     /translation="MSEESDMERSEPASPRRLEKSRDEGQVPRSVELSTFAILMAAGS
                     GLWLMSGHITSQLMDLMRDGMKLPPAAGFDTGLLGERLLDQSLHALVALAPYLMLIFV
                     VGLLAPMLLSGWLFSPKALAPDFNRINPLKGLARIFSLHGLAELVKALLKATLIGTIG
                     AWTIWHHKETVLSLISFPFAAGAVHMGELVAMSFLTIAAAMAFIAAVDVPFRLWDHHR
                     KLKMTKEEVRQENKETEGDPHIKARIRAQQRSMARRRMMAQIPSADVVVTNPAHYSVA
                     LKYEEGKMRAPRVVAKGAHLVALRIREAAREHQVPLLEAPPLARALYHHAELGDEIPQ
                     TLYNALAEVLAYVYQLRRYRQSEESGQNSGPAPQRPSDLPVPPELDPGQ"
     gene            complement(7780..8703)
                     /gene="motB"
                     /locus_tag="VFI43_07970"
     CDS             complement(7780..8703)
                     /gene="motB"
                     /locus_tag="VFI43_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HET7062101.1"
                     /translation="MAEAPRPIVIKKIKKYHRGHGGQWKIAYADFITAMMAFFLLMWL
                     LGSSTKAELKGIAEYFQSPLQVSPKGGTGSEDGTSVLQGGGRDLLRSHGRTSGQNSES
                     GKPVNLKAAQAELERIDALRLEDLKRRLEQLIESNPVMRQYKKQLLLDITTEGLRIQI
                     VDDKNRPMFALSRAELEPHTRDILQKLGGALNEIPNKISLSGHTDAKPYPTGEKNYSN
                     WELSADRANASRRELILGGMQEQKMLRVVGLSSAVLFDKDDPANPVNRRISIIVMSRK
                     AENALTGEDGQIKQIRRDAPIQAQALQYHGG"
     gene            complement(8829..9689)
                     /gene="motA"
                     /locus_tag="VFI43_07975"
     CDS             complement(8829..9689)
                     /gene="motA"
                     /locus_tag="VFI43_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HET7062102.1"
                     /translation="MLVIVGFIIVVASIFGGYMLAGGHIAALYQPLELLIIGGGAAGA
                     FVLGNTHKAQKATLKALPKVLRGSRYNKAIYMDLMALLYTLLAKVRKEGLMSIEVDVD
                     KPEESEIFTQYPSILSDPEVVEFMTDYLRLMVGGNLNAFEIENLMDNEIETHHQEGEV
                     PVHCITKLADSMPAFGIVAAVMGVVHTMASVGLPPSELGELIAQALVGTFLGILMGYG
                     FVGPFAAVLEQKLSESTKMLQCVRVTLLASINGYAPALAVEFGRKVLYSTERPSFSEL
                     DEHVRQAKKK"
     gene            complement(10371..10919)
                     /gene="flhC"
                     /locus_tag="VFI43_07980"
     CDS             complement(10371..10919)
                     /gene="flhC"
                     /locus_tag="VFI43_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HET7062103.1"
                     /translation="MRKKSVISEAREIQIAIEMIRLGARLQLLETETNLSRERLIKLY
                     KELKGVSPPKGMLPFSADWFLTWQPNVHSSIFAGIYQYLTKHGGCQGIAAIVKSYKLY
                     LEHCEVTEMEPVLSMTRAWTLVRFFEAKMLQTAPCKKCSGHFVVHSNDLHGRYVCGLC
                     HMPSRAGKTNKSRIAALAVAAA"
     gene            complement(10942..11259)
                     /gene="flhD"
                     /locus_tag="VFI43_07985"
     CDS             complement(10942..11259)
                     /gene="flhD"
                     /locus_tag="VFI43_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HET7062104.1"
                     /translation="MKSSELTTEIKELNLTYLMLAQQMVISDKDMAIFRLGISRDIAD
                     ILEVLTPGQIVKLSNSNMMLCRIRFDDNLIFGMLANYNKDRAMAQSHAAILMAGQPAE
                     VIS"
     gene            complement(11564..12772)
                     /locus_tag="VFI43_07990"
     CDS             complement(11564..12772)
                     /locus_tag="VFI43_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062105.1"
                     /translation="MTRYIERLAVVATLAVAAVCSLQGALAGKVSVDVGRSNAPIFTT
                     KEKFIFLLKTDFGELWKHRAPPNTEVPDEIKDFEMPGSLSWTDKAGKHELPVDVHLRG
                     NTSQSEAQCPFPKMTLTFKKDRDGERVSKGTLFDGLKTVGIGTHCGGKPGSSVRFHRV
                     WGGLSPHREALVYKVQALLSISGFEAAPASFSYIDSKTGKGPMEPRPSGFIVERSMPG
                     FFLEDIKTFVKYADGKEIRAADASLKDSDKAYIFTSVVNAQKDAAAAGSTIEPIHPKA
                     VIRILLFQSLVGNYDWHLRINPDDIPDSQGLWNIKVVETDGNWIIFPYDYDLSGWVKS
                     ADGSPEMPDLAPRFFKPDDVAKVVAEFREKRPAIEALVDELASEDRAGAQSMKRQIDS
                     FYKELNKKFH"
     gene            13649..13737
                     /locus_tag="VFI43_07995"
     tRNA            13649..13737
                     /locus_tag="VFI43_07995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13683..13685,aa:Leu,seq:taa)
BASE COUNT         3064 a         3881 c         3667 g         3188 t
ORIGIN      
        1 aacaactgac cggttcggcg cactgccgaa agcgctgccg agggaatttg caaccattcg
       61 gtacttaccc tcgtttacct gggagatggc gtacagcacc acaaagagcg cgagcaggag
      121 agtgatgaaa tctgagtaag agatgagcca gcggtcatgc ttttcactgt cttcctttat
      181 atggcgtcgc ggcattatgg gatgggtagg cgagcgacgg attacaccag gtatccgttc
      241 agccgccctg taatcaggcg aggattttct cccctggcta tgcctaccag cccgtccacc
      301 agtatttctc gcatcaatac ctgccgggac acgagcgcct tcagcttgtt tgcgacgggg
      361 agaaatatca ggttggcgag ccccacgccg tagagcgtcg cgacaaacgc gacggcgatg
      421 cctgccccca agcccgccgg gtcggacaga ttctccatca cctggatgag tcccatgaca
      481 gcgccgagga tgcctatggt cggagcgtat cctccggctg cttcccacac tctggccccc
      541 tggcgatgca gatcatcaaa tgactcgatc tccacctcga gtatttgctt gagaactgct
      601 ggttcaatcc catccaccaa tagttgcaat ccttttctta taaaaggatc atcaataccc
      661 tcgatgtgcg tgtccagggc aagtaacccg tcctttcgcg agatcgagct ccaggagccg
      721 atgtgatgca ccagccccgc ctcgtcgagc agcggcggga tcaaaaccca tctgctcatt
      781 ttcatgccga gcaaaaattg cctaatcgga ctctgcagca ggactgcgcc gagcgtgccg
      841 ccgagcacga caccaagtga tgtaaactgg atgagtgaag cgacgctccc tccctccagg
      901 gcctggccgc caagcaccgc gatcagcgcg atgagaattc ctgttatgct gctgaagtcc
      961 acgcgcattc cttgagcctc gctctcagcc gggcgattgc ctggctgtgt atctggcata
     1021 ctcttgactc cgatacgcca agaacagcgc cgatctcctt gaaattgagc tcctgctcgt
     1081 aatagaggcc catgagcgtt ttttcgcgct ctggaagcgc atttatcgcc gcgataaggg
     1141 attgccgcag gtcgccgtcc agcagaccca ccaatggatc ggcggcgttg tccgcgcaat
     1201 ggcgttccag gtagtcgtct tcctcgttct cgccaaaatc ctcatagtgc accaactgca
     1261 ccccccgcgc ctcaaaaagc atttcgtggt aggtatcgac cgataccccg agttcggctg
     1321 cgatttcgac ctctgtcggc gaacggtact ggcgctgctg gaggacgctg atggctgctt
     1381 cgatttccct catattcttg cgcgcgctgc gcggcagcca atccatgtgg cgcaattcat
     1441 ccaacattgc tccccgaatg cgctgctggg cgtacgtcgc aaattgagcg cccggcattt
     1501 gctcgtagcg atccagcgcc tccatcaagc ccatcattcc cacttggatc agatcatcga
     1561 tttcgacgct gacgggtagc ttggcgataa gatgctgtgc aattcgcttt accagaggcg
     1621 cgaacctggt cagatgctgc tgcttgtcaa aaataccggc agaggtatac atggattcac
     1681 cttaaccctg aaatgactgc ttgtgcccgt tcggatcgac gggcacgact gttctgcagt
     1741 aaacgctgaa tgaagcgttc cagcattctg ctctcgcctt cccggcagga ccatttgccc
     1801 aatgattccg cagcctcccg gaatgccgct gccgttgggg tatccggaaa cgcctcagcc
     1861 acaggcaaac ccaggctgag ctttccgtcg gtcgggatgt atccggtgaa atcaagagag
     1921 aggccgaggt attggtcggc agcccgcgcc atgttttcga aaacagtccc tgcttctctt
     1981 tccatcgagc ttttattgat caatatatga aaacgctgct tgtccgcgtg ataaccgtgc
     2041 acgtgcttga tgagggaata ggccgaggtg atggaagcag gctccggagc gaggacgaca
     2101 agaagctcgt ggccagcgaa atcaagtgaa agcaatcggc tggatcggcc gggcgcagtg
     2161 tcaatcagta cgatgtccat gggttgagca atctgagcaa agcagccgat cagatgcacc
     2221 tgatcgcccg tgttgagctt gtccagtaca cgcattccac ggcccgccgg cagaatctga
     2281 aagcccagtg gcgcagaaat caagacctga gccaaagttt tgtccctgcg gataacatcc
     2341 agcaaatccc ggtgcgcgct taaacccaat gtcgcgcaaa tattgcttgt gcaggcattc
     2401 tcatcgattg ccaagacgtt ttttcccgat gcggcgagaa atgcagcgag attgataatt
     2461 gacgtcgtct tgcccgcgcc ggcaccaccg gaaacgacgc tgacaatacg tgtggaatcc
     2521 tgcatcagca gacgacgcaa cccttcggcc tgatcatgtc cggtatcagt caagcggaac
     2581 tcctgccgca ttgccaatgg agcgcgtgac acgcccgacc atgggcagga ataaatccgg
     2641 cggtaatgaa tctgcaaaag caggtgaaat ttcgagcgca gcctcgatca gcttcccccg
     2701 atcggccaat tgaaggtctt ccggaacacg ctgtcctgtg gaaacataat agagaggcag
     2761 acgatgacgg atggccgcct caagagcgcc tccacttaca actgcctcat caatcttggt
     2821 aatgatggcg cccgccaagc cattgccgcg atatgctccc gcaacttcat ccagggtttg
     2881 caagcttgac gaagcattca gcagcagtat gcggctgatt tcctcatcgc actctgtgaa
     2941 catggccact tgctccgcca ccatctcatc gcgttggctc atacccaccg tatcgatcag
     3001 gaccatgtgc ttgcctcgaa gttccgacaa tatcactgcg agatcttgcg catctcgcac
     3061 tgtgtgtacc gggactccaa gaattttgcc gtatattcgt aactgttcat gaccaccgat
     3121 acgataaccg tcggtagtaa gcaaggcgag cttgtctgcc ccgtggcgaa cgacgcagcg
     3181 tgccgcgagc ttggccgtgg ttgtcgtctt gcctaccccg gtcgggccca cgagcgcgta
     3241 gattcctcct ttttcaagga tttcgtcctc actctcgacg gtctccaggt tttccgcgag
     3301 caagcggtga acatgcgcca tggcttgctt ttcgtcgttt tgcatatcgg aaatatgagc
     3361 cgaccggtcg agcagacggt gtgcgaagcg agcgctgaaa cctgcatcca gcaacctcga
     3421 taccaggttc cgtcgaaccg gcgcaccatg tgcctcacca ctccggttca gcaacgcgag
     3481 ctgttgctcc agggcgctgc gcatcgaatt gatttccctg attacgctgg cgcttacctc
     3541 ctcagccaac cggtgcgtgt caaacacagg ggcctctctc gatttcgcag cgctttcgga
     3601 cgatataatc gcgtctgttc tcgacaacga tgaactgcgc ttttttctac cgttatcgat
     3661 ctcgagcggc gagctttcaa actggctcag gggtgggttg cgacgttttt gggatggacg
     3721 atcgttgagc ggctcgtccg gctgggctgg ctcccgaagg ccggagccgg tggagtcacc
     3781 tgcggatgtc ttcgggtgct gaattggaaa gtcaggtagg ggcgcgcgat gctttttttc
     3841 catctttcct gaagacacgg cattctctcg ggatggataa ttttcaggag gagttggcag
     3901 ttcgacacgt ttcagcgaat ctttccagct ggaaaatgat actgtttgcg tcccattcct
     3961 cgattcatcc ctcctcgatt catccctcct tggcctgctc tccttatctg aaaggatgcc
     4021 aggggcgtgg cggaaagcgc gtggatttaa gtcttcaaat cctgctgctt cgtgaggcgc
     4081 tgaagacagg tggctcggaa tgagcgacga gatatggtca tggtgcgcag ccaggatctc
     4141 gattccgccg tcgatatcac gattggacag gatgattgca tctgggccga gcacgtcgcg
     4201 cacctttcgc aacgcttcgc ttgtcgtcct ggcaaaaaac ttctggatgc tcatgtcctc
     4261 gctccaagga tggaagtgac tttaatactg gctgaatcgg gcagctcagc gtgagaaatt
     4321 atcttgagtt gcggcagtgc ccgccgcagg aagcgcgcca gtagcgccct cagtggcgcc
     4381 gccaccagca atacgggcgt caatcccaac tgctccagcc gcttgctgcc cccggatgct
     4441 tcccggagaa gagtttcggc gagcccaggc tcgactccag cgccctgagg gctgctctgc
     4501 agcgcttgcg tcagcaatcg ttccaacccg gaatccagcg ccattacctg cagctcggtg
     4561 gattggggcc atatctgctg aacgattgcg cgacccagag caatcctgac cagtgcagtc
     4621 agctcgtgcg ggtcctgcac gcgaggcgcg tgctcggcaa gggtttcaac aattgtccgc
     4681 aaatcgcgga cgtggacatt ttcctcgaga agattctgca agaccttttg cacggaagcg
     4741 agcggcagaa atttgggtgt gagatcttct gtcagcttgg gagattcggt tgccaggcgg
     4801 tcaagaagct gctgaacttc cgtgcgcccg agcagttcag cggaatggcc atgtataaga
     4861 tggttcaaat gcgtggctac gacggtgcct gcgtcaacca ccgtgtagcc cgttatttgc
     4921 gcgtgttcgc gctggctcgc atcgatccat gtcgcgggca gtccaaatgc cggatcgctc
     4981 gtggcctgac cggtcagatt cccggaaact tgccccgggt ttatcgccag gtaatgaccc
     5041 acgctgacct ctccgctgcc gatagcaacc cctttcaggg taatgcgata accgccgggc
     5101 ctcagatcga ggttgtcgcg aatatgcacg ggcggaggaa gaaaaccgat ttcttgggcg
     5161 aatttcttgc gaacgccctt gactcgccgc agcagttcgc cgtcctgcgt cttatccacg
     5221 agtgggatca agcggtagcc tacttcgagg ccaagaatat ccaccggcac gacgtcatcc
     5281 cagcttgcgt ccttcacttc cggttgcacg tttggcgccg ctggcgcctt tattgccggc
     5341 gtcgcctgcc gccgtatcat cgaatacgcc cctcccgcaa gcgccccggc cagcaacagg
     5401 aatgcaaaat gcggcatgcc aggaatgagc cccatgaagc cgacgatcgc cgcagtcagc
     5461 gccagcacgc gtggcctcga aaacaattgg cttgccagtt gcgtgccaat atcctcgtcg
     5521 gttcctacac ggctcaccac gaggcccgcg gccgtcgaaa tgatgagcgc tggaatctgg
     5581 gctacgaggc cgtctccaat ggcaagcagg gtatagtttc tggcagcggt cgagagatcc
     5641 aggtcgtgct gaagcactcc tatgaccagg ccgccaataa catttataaa aagaatgaga
     5701 atgccggcta tcgcgtcacc ccggacgaac ttgctcgctc catccatcga accatagaac
     5761 tcggcttcct gcgcgatcgt agtgcggcgc ctgcctgctt cctcctcccc gatgaggcca
     5821 gcattgagat ctgcgtcgat cgccatctgt ttgccgggca ttgcatccag agtgaagcgc
     5881 gcggaaactt ctgcgatgcg accggcaccc ttggtaatga caataaagtt gattgccacc
     5941 aggataatga acacgacaat gcctaccgtg taattgccgc ccacgagata gtggccgaac
     6001 gcctcgatca ccttccctgc cgcgtcgggc ccactatggc cttccatcag cacgatgcgg
     6061 gttgaagcca cattgagcga cagccgtaat agcgtggaaa gcagcaggac ggtgggaaac
     6121 acggcgaaat cgagcggctt gtgcgtgtgg atgctgacga gcagcaccat gatggcaagg
     6181 gcgatgttga acgtgaacat gatatcgagc aggaatgccg gaagcggcag taccatcatg
     6241 cccaggatca ggaagataag cagcgggctg gcgaaactgc cggccttgat cctgttggcg
     6301 tccttcgcat cgttgaagcc gaagacgttg gccagtttgg caatcgaatt catggatgga
     6361 ttaatattcc gagtgtgcca tcgtattact gtccgggatc gagctcaggc ggcaccggca
     6421 aatcggatgg cctctgtggc gcgggacccg aattctggcc cgactcttca gattggcgat
     6481 agcggcgaag ctgatagaca taggcgagca cttccgccaa ggcgttgtag agtgtttgcg
     6541 gtatctcgtc gcccagttcc gcgtggtgat agagcgcgcg tgccagtggc ggggcttcga
     6601 gcaggggaac ctgatgttcc ctggccgctt ccctgattct cagggcaacc agatgcgcgc
     6661 ctttcgccac aacccgcgga gcgcgcattt ttccttcctc gtacttgagc gctaccgaat
     6721 aatgagcggg attggtgacg accacatcgg cggatggaat ctgcgccatc attcgtcttc
     6781 ttgccatcga ccgctgctgg gcgcgaatac gggccttgat gtgcggatca ccttccgttt
     6841 ccttgttttc ctgccgcacc tcctccttgg tcatcttgag tttgcggtgg tgatcccata
     6901 agcggaacgg gacatcaacc gctgcaatga atgccatcgc cgctgctatg gtcagaaaac
     6961 tcattgccac cagctcgccc atatggaccg ctccggctgc gaaggggaaa ctgataagtg
     7021 atagcaccgt ttccttatgg tgccagatgg tccacgctcc tatggtcccg ataagagtgg
     7081 ccttgaggag cgccttgacg agttcggcca gtccatgcag cgagaatatg cgcgccagac
     7141 ccttgagtgg attgatccgg ttgaaatcag gggcgagtgc ctttggactg aaaagccacc
     7201 cggaaagcag cattggcgcg aggaggccga ccacaaatat caacatcagg tagggagcga
     7261 gcgcaaccag tgcatgcagg gattggtcaa gcaagcgctc tcccagcaat cctgtgtcga
     7321 acccggctgc cggcggcaac ttcatgccat ccctcatcaa atccatcagc tgagaggtaa
     7381 tgtgcccgct catcagccac aggccactgc cggcagccat caagatggcg aacgtggaca
     7441 gctccacgga acgcggcacc tgcccctcgt cacgcgactt ttccagccgc cgtggtgacg
     7501 caggctccga acgttccata tcactttctt cactcaccgc gacttgtctc tcaaatgcac
     7561 tcagccgtat cagctgatct gcattatcaa gcggtgagga actgcagtat tgaaggaaaa
     7621 gagccgaatt agccggtcaa tttgggtggg gggtgggatt ggggactacc aagagtttta
     7681 ccgctactcg cgacggagat gctgctccgt gggatggcgc gaaatgggaa cgcctgaagg
     7741 tgccgcatga tgggaatcaa atatattact gctcgtgcgc tagccgccgt gatactgaag
     7801 agcctgcgcc tggattgggg catctctcct gatctgttta atctggccgt cctccccggt
     7861 cagcgcgttc tcggcctttc tgctcatcac aatgatactg atccgccggt ttacaggatt
     7921 ggccggatcg tccttgtcaa acagaaccgc cgagctgaga cccactacgc gcaacatttt
     7981 ctgttcctgc atccctccca gtatgagttc acgccgggaa gcgtttgccc tgtcggcgga
     8041 caactcccag ttactgtagt ttttctcccc ggtaggatac ggtttggcat cggtatggcc
     8101 ggaaagactg atcttgttgg gtatctcgtt cagcgctcca cctagctttt gcaggatatc
     8161 tcgggtatgc ggttccagtt ccgcccggga gagcgcaaac atggggcgat tcttgtcatc
     8221 gacgatctga atgcgtaacc cttcggtagt aatatcaagc agcagttgct tcttgtattg
     8281 ccgcatgacc ggatttgact cgatcaattg ctccagcctt ctcttcaaat cttccagtct
     8341 cagcgcatcg attctctcga gctcggcctg cgcagccttc agattgacgg gttttcccga
     8401 ttcgctattc tgtccactgg tacgcccgtg gctccgcagc agatccctgc ccccgccctg
     8461 caggacgctg gtaccgtctt cgctgcctgt gccgcctttg ggcgagactt gcagcggaga
     8521 ctggaaatat tccgcaatgc ctttcaactc ggccttcgtc gaggatccaa gcagccacat
     8581 gagcagaaag aacgccatca tggccgtgat gaaatccgcg tacgcaatct tccactgccc
     8641 accatgaccg cggtgatact tcttgatctt ttttatgaca atgggacgcg gtgcttcagc
     8701 catgggaatt tttcaggata gttttggatg ccgagccaat ttctaccggc aaatgctggc
     8761 tgaacgtgaa gagttcgcgg cctgaatacg gccgtgatac tggagaaagc cggtggcatt
     8821 ttcttcaatc attttttctt ggcctgtctg acatgctcat ccagctctga aaaactgggc
     8881 cgttcggtcg agtagagcac cttgcgtcca aactcgactg caagcgcagg agcatatccg
     8941 ttgatactcg ccagaagggt taccctgacg cattgcagca ttttggtgga ttcgctcagc
     9001 ttttgctcga gaacagcggc aaaaggaccg acaaacccat aacccatgag aattccgagg
     9061 aatgtgccga cgagcgcctg tgcgatcaac tcgcccaact cggatggagg aagtcccacg
     9121 gacgccattg tatggacaac acccattacc gccgcgacga tgccgaatgc cggcatgctg
     9181 tctgcgagct tggtgatgca atgaacgggt acctctcctt cctgatggtg tgtctcgatt
     9241 tcgttatcca tcaggttctc gatttcgaaa gcattaagat tgccgccgac catcaggcgc
     9301 aggtaatccg tcatgaattc cacgacttcg ggatccgaaa ggatgctggg gtattgggtg
     9361 aatatctcgc tttcctcagg tttatccaca tccacttcga tggacatcag cccttccttg
     9421 cgtactttcg ccagcagtgt atagagcaat gccatcaagt ccatgtagat cgccttgtta
     9481 taccgcgaac cccgcaggac tttgggcaat gctttcaagg tcgctttctg tgccttatga
     9541 gtattgccaa gaacgaacgc tccagccgcc ccgccaccta tgatcagtag ttccagcggt
     9601 tggtatagcg ccgcaatatg ccccccggca agcatgtacc caccgaaaat cgatgctacg
     9661 acgatgataa atccaacgat gaccagcaat ttgcagaccg cctgcttgaa atggttactc
     9721 gggcttccac cgtcgctacg catttcacct tgggctgcct gccggaaggg agagtgtgcg
     9781 aacgcgtggg atatccctat taacgccggg aggagagcaa actttagggg ggtcaaagga
     9841 tgcggttgga aacctgcttc tcgactccac ccgtgttggg tggcagtcca aaacgaatga
     9901 tcagttggag ggtgaggctg gctaaaagct cctggatgga gaaagcctgg cagcaatagt
     9961 taactttttg cttctactct cgttaagtta atctgtcttg aatgagaacg atttgcatta
    10021 attgcattaa ttgcattaat cgaatgaaat agacaagcgg tataaacctg gcgacgaaca
    10081 tcgcgtcccg cgaaggggtg aacgctccga gtataaggac gttccttgcc cccaatttca
    10141 tcagcccact gagctcaccc agtaatgcct tgcgttcttc catcctgagc aggaacagcg
    10201 gcgtatgaat attctgtttt tcccgcctgt cgcgttatcc ggcaacctgc cgtcttccag
    10261 cgcaggaatg cgtggctttg cagaaagccg tgaaatccat tccaggggct tgaaaaagaa
    10321 aaagctcccg gccgccgtgg ggttggcagc caggagccgg agggaattat tcaggctgct
    10381 gcgacggcga gcgcagcgat tcttgacttg tttgtcttgc ctgcgcgaga gggcatgtgg
    10441 cacagcccgc acacatatcg accgtgaaga tcgttgctgt gtacaacaaa atgtccactg
    10501 cactttttgc agggggcggt ttgcagcatt ttggcctcaa agaatcgcac gagagtccac
    10561 gcacgggtca tgctcagcac cggctccatt tccgtcacct cgcaatgctc cagatatagc
    10621 ttatagctct tgacgatggc agctatgccc tggcaaccgc catgtttggt gaggtattga
    10681 taaatacctg caaagatcga cgagtgaaca ttgggttgcc aggtaagaaa ccaatcagca
    10741 gaaaatggaa gcataccctt gggaggcgac acccctttca attccttgta gagtttgatg
    10801 aggcgttccc gactgaggtt ggtctcggtt tcaagcaatt gcaaacgggc gccgagacgg
    10861 atcatttcaa tcgcgatctg gatctcgcgg gcttcggata tgacactctt tttacgcatg
    10921 atgtgactcc tcgtattcga ttcaggaaat tacttcggcc ggctggcccg ccataaggat
    10981 cgccgcgtgt gactgcgcca ttgctctatc cttattgtaa ttcgcaagca tgccaaagat
    11041 gaggttgtcg tcaaagcgaa tgcggcaaag catcatattg gaattcgaca atttcacgat
    11101 ctgcccgggt gtcagcactt caagtatgtc tgcaatatca cggcttattc ccaaccggaa
    11161 aatagccatg tccttatcag agatgaccat ctgctgcgcc agcatcagat aggttaagtt
    11221 caattccttg atctcggttg tcagttcgct ggatttcatt tttcccccct gttgatgtga
    11281 tggcgtcatt ttgcacacct caaagggagc tttacactcg aacttatccg atttgcaggt
    11341 aggagaatca ggatgattga tgtaggaaaa tttcctacag ggtattagcg aggaaacgct
    11401 ggagcttcag tatggaaaat gccccggtac acggaaagct gatgtagcct cacttgaggt
    11461 ctttcatcta cggcatcgcg tcggaaagct ttagacgaac aggtaaaact tatgcctctg
    11521 tcaatccaac gcagacctcg agccaattct tcaacgcgac gtatcaatga aactttttgt
    11581 tcaattcctt atagaacgag tcgatctggc gcttcatgct ctgcgcccca gccctgtcct
    11641 cactggccaa ctcatccacc aaagcctcga tggccggccg cttttccctg aactcggcca
    11701 caaccttggc cacgtcatcc ggcttgaaaa agcgtggggc cagatcaggc atttcgggcg
    11761 aaccgtccgc agattttacc cagccagaca gatcgtaatc gtatggaaag atgatccaat
    11821 tcccgtcagt ttccacgacc ttgatattcc atagcccttg tgagtcaggg atatcgtccg
    11881 ggttaatgcg cagatgccaa tcgtaatttc cgaccagaga ctggaacagc agaatccgta
    11941 tgacagcttt gggatgaatg ggctcaatgg ttgaacccgc cgctgctgca tccttttgcg
    12001 cattcaccac gctcgtgaat atgtaagcct tgtccgaatc cttgagactt gcgtctgcgg
    12061 cacgaatctc cttgccgtca gcgtatttga caaaagtctt gatatcctcg aggaaaaagc
    12121 cgggcatcga ccgctccaca atgaatccgc ttggtcgggg ctccatcggt ccctttccgg
    12181 ttttgctatc gatgtagctg aagcttgccg gcgccgcttc aaaacccgat atcgaaagaa
    12241 gtgcctgcac cttgtaaacc aatgcctccc ggtgtggcga aagacctccc catactcgat
    12301 ggaagcgaac cgaagagccc ggcttgccgc cgcaatgcgt tccgatacca accgttttca
    12361 ggccgtcaaa cagcgttccc ttggaaactc gttccccgtc tcggtctttt ttgaaagtca
    12421 gggtcatttt gggaaagggg cattgcgctt cggactggct ggtgttccct cgcaaatgca
    12481 catccacggg caactcatgc ttgcccgcct tgtctgtcca cgaaagcgat ccgggcatct
    12541 caaaatcctt gatctcatca ggcacttcgg tattcggagg ggcccgatgc ttccacaact
    12601 ccccaaaatc ggtttttagc aaaaatataa acttttcttt cgtggtaaag atcggcgcgt
    12661 tgctgcgccc cacatccacc gaaactttgc ctgcgagagc tccttgcaaa gagcaaacgg
    12721 ccgcgacagc gagggttgcg acaacggcca gcctctcaat atatcttgtc atcgaacctc
    12781 cagcagaatt gataaaggcg gccagggcat tgatcgatca tgctcaccca gaagaatgaa
    12841 atttaatgct aggaaattgt tgcgtctttt caacttggcg ccagaaaata gcactgctga
    12901 tttgcactct ttttttttac aggaacagga aaagatggcc gggaaaatct acctaagaag
    12961 tagaaatgat ctattcttct agaagtttca aagcacgcct gagcttgtgg aggtgcgcgt
    13021 cggctgaata gcgaacttca agaggcaggg ataaaaaatt tacgatgaat atcaagaaaa
    13081 aattcacgaa aagaccctaa gaagagcagg tacatttaga atgtctgcaa ttgcacgttc
    13141 cgcttcaata ttttagggaa gggttcggct atgaagttcg agtggctccg cgatgacctg
    13201 aaaggtaatc atttactatg ccttgctgaa cagagaagct tgtttttccg tggaacactc
    13261 tccccggaaa gagcgggcaa gcgattctgg caaccgttcc ccgaagggga attacaggaa
    13321 gttagtgccg atttgaaaag cgcacagcgc cagtgcgagg aaatcgtatc ttcaacggag
    13381 agcgcagata tcggtatgtg atgcgacgtg ttccgactcc agcacggaga gttaaggtag
    13441 ccaccctcat cacccgccac ccaaatcctt accgccgcgc gtgcttcaag ctgcgtccac
    13501 cagcttttta aatagaacaa tacagcttcc cttggttact tttgacgcca aacatgggcc
    13561 tctctgagaa gcattgcacg ccgcgccttg tgataacata gcgcgggctg acgcccttat
    13621 ccgcttctcc agcgtcccac ataaatccgc ccggatggtg aaattggtag acacaaggga
    13681 cttaaaatcc ctcgggctta accgcccgtg ccggttcgat tccggctccg ggcaccaatt
    13741 gtagattatt attcaacagc ttatctaaag catagtcttc ttgtggtggt actttcatgg
//
LOCUS       DASSHJ010000205         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_367708, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000205 DASSHJ010000000
VERSION     DASSHJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367708"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..960
                     /locus_tag="VFI43_08000"
     CDS             157..960
                     /locus_tag="VFI43_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7062106.1"
                     /translation="MERPTTSRFTRILRSVRLLLHLVSGFAQAAVYPHVSQRIQKRMA
                     QNWSAGLLSALSIRLRYSGPVPDPVAPRVMLAANHVSWLDVYSLISVCPARFVAKSEI
                     GGWPLLGWFSKNAGTLFIERSKRSDTARINEHIADAVISGDRVAVFPEGTTGNGTFLR
                     HFHASLLQPAVAVGATLFPVAIRYTNTRGQISEAAVYVNISLMESLRMVLREPWIQVE
                     LIFTDPISSAEKNRRELARSAESAIAAALSLPLPHKGPGSPPYRPGEQP"
     gene            complement(881..1708)
                     /locus_tag="VFI43_08005"
     CDS             complement(881..1708)
                     /locus_tag="VFI43_08005"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HET7062107.1"
                     /translation="MPIFAIGDLQGCYSSFRQLIDRIQFDPARDRLWLVGDLVNRGPD
                     SLATLRFVKELGNAAVTVLGNHDLHLLMVAAGCARLRPDDTLEDILAAPDREELLDWL
                     RHREMLHVEGDHVLVHAGLLPSWSVQKASELARMVEDGFRSTNFHEFCANMYGNQPDQ
                     WSDELEGHERSRVVINAMTRMRVCTRDGKMNFSYKGPPQDAPPGYLPWFDVPGRASQE
                     ATIICGHWSALGLRMEENLIALDTACVWGKALTAVRLDDREVIQVPCGGGEGTTRRQ"
     gene            complement(1784..2332)
                     /locus_tag="VFI43_08010"
     CDS             complement(1784..2332)
                     /locus_tag="VFI43_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7062108.1"
                     /translation="MVSGTEDEEHSSAIKTPEQLIAVLVAAFVFPVLIILLLARLAIG
                     TAPVEREGDAFSDQAVAARLKPVGTLVLAGTPEAEAAPAASAVNAASAPPSASAPASA
                     EDKAKAVYTSSCAACHMTGAAGAPKLGDKAAWAPRIKTGAESLYTSALKGKNAMPPKG
                     GNASLSDSDVKSAVDYMVSQAK"
     gene            2467..2543
                     /locus_tag="VFI43_08015"
     tRNA            2467..2543
                     /locus_tag="VFI43_08015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2501..2503,aa:Arg,seq:ccg)
     gene            complement(2982..>3559)
                     /locus_tag="VFI43_08020"
     CDS             complement(2982..>3559)
                     /locus_tag="VFI43_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HET7062109.1"
                     /translation="RCREPMFGISNRIAYDGQMVYAAGSHEAGTIGRVLGPSTWLNID
                     GEADSKWSPAEGELVVHLLKKIASAGITEPDLFIITPFQIVAQELRRRLGREAALFAA
                     FKADVREWTTDRVGTIHTVQGREADTVILVLGAPKASQNGARGWAAGTPNILNVAVSR
                     AKQNLYVVGSYGAWSGVGHAREVATMPRRRV"
BASE COUNT          769 a         1052 c          934 g          804 t
ORIGIN      
        1 attgtttcac tcgagatgaa atcgcacaac gctatttcga taattttggc tcacggattc
       61 tataggtaac agaaggatcc ggaacaagct ctccaggagg aaatgcagct ccaggcggca
      121 ttttggattc tcgtcgccgg atgtcataat aatggcatgg aacgtccaac caccagccgt
      181 tttacaagaa ttcttcgttc cgttcgcttg ctgctgcatc ttgtttcggg gtttgcccaa
      241 gctgcggtct acccccatgt cagccagcgc attcaaaaac gaatggccca aaactggtcg
      301 gccggcttgc tctccgccct gagcatccgg ttgcgctaca gtgggccggt tccggaccca
      361 gtggcacccc gcgtcatgct ggcagccaat catgtttcct ggctcgacgt ttactctttg
      421 atatccgtgt gccccgcgcg cttcgtagcc aaatcggaaa ttggcggatg gccgctgctc
      481 ggctggttta gcaaaaatgc gggaacattg ttcattgagc ggtcaaagcg cagcgatacc
      541 gctcgaatca acgaacatat cgcagatgcc gtcatcagcg gcgaccgggt tgctgtattt
      601 ccggaaggaa ccactggcaa cggcaccttc cttcgccatt ttcacgcctc gctcctgcaa
      661 cccgcggtag cagtgggcgc cacgctgttt cccgttgcga tacgttatac caacactagg
      721 gggcagatca gcgaggcagc cgtctatgtg aatatctcgc tcatggaatc attacgtatg
      781 gtactaaggg aaccgtggat tcaggtagaa ctgatattta ccgacccgat aagcagcgct
      841 gaaaaaaatc ggcgcgaact cgcacggagc gcagaatcag ctattgccgc cgcgttgtcc
      901 cttcccctcc cccacaaggg acctggatca cctccctatc gtccaggcga acagccgtaa
      961 gagccttccc ccaaacgcag gcagtatcga gggcaatcag gttttcctcc atgcgcagcc
     1021 ccagggcgga ccaatgcccg cagatgattg ttgcttcctg gcttgccctc cctggaacgt
     1081 caaaccacgg caaatagcca gggggagcgt cctgcggcgg ccccttgtaa gaaaaattca
     1141 tttttccatc ccgggtgcaa acacgcatac gtgtcatcgc gttgatgacc acccgggatc
     1201 gctcatgacc ttcaagctca tcgctccatt ggtcgggttg attgccgtac atgttcgcgc
     1261 aaaattcatg gaaatttgtg ctgcggaaac cgtcttccac catccttgcc agttccgagg
     1321 ctttttgcac ggaccaggag gggagcaacc cggcatgcac cagaacgtgg tctccttcga
     1381 cgtgcagcat ttccctgtgt cgcaaccaat cgagcaactc ctccctgtcc ggtgctgcga
     1441 gaatatcttc gagggtgtcg tcagggcgca atcttgcaca tcccgcagcc accatgagca
     1501 gatgaaggtc atggtttccc aacactgtga cggccgcgtt gcccagttct ttcacaaaac
     1561 gcaaggttgc cagtgaatca gggccgcggt tgacaagatc gccgacaagc catagccggt
     1621 ctcttgccgg gtcaaactgg atccggtcga taagttgtcg gaacgagcta tagcagccct
     1681 gtaagtcgcc aatcgcaaaa atgggcattg gctccgggta tcaaccctat aagtgcgtgt
     1741 ggaaagggtt acgcgtggta attcgcgaca cggttgattc gctctacttg gcttggctta
     1801 ccatatagtc caccgccgat ttaacgtctg aatcggaaag gcttgcgttt cctcctttgg
     1861 gtggcatggc gttcttgccc ttaagcgcac tggtatagag cgattccgcc ccagtcttga
     1921 ttcttggcgc ccacgctgcc ttatcgccca gtttcggcgc acctgcagcc ccggtcatgt
     1981 ggcacgcggc gcagctcgaa gtgtaaacgg cctttgcctt atcttctgcg cttgctggcg
     2041 ccgaagctga aggtggtgcg cttgcggcat ttacagcact agcggcggga gccgcctcag
     2101 cctcgggggt tcctgccaga accagggtgc cgactggttt caaacgtgcc gctacggcct
     2161 ggtcggaaaa tgcatcgcct tcgcgctcga ccggggcagt ccctattgcg aggcgagcca
     2221 gaagcagaat aatcaggaca ggaaaaacga aagcggccac gagaaccgcg atcaactgct
     2281 ccggcgtttt tatggccgac gaatgctctt catcttctgt gccgctcacc ataacccccc
     2341 aagaaaaaga ggttattgta tagattcatc cttatcgaag caaaaacaac ctatcggctc
     2401 cgttggacgg aggaaccaaa aaacgctatt ctattaaccg aatcggtatc cttgattacc
     2461 tcacttgcgc ccatagctca gatggataga gtaccgccct ccgaaggcgg gggtcacacg
     2521 ttcgaatcgt gttgggcgcg ccagttgctg cttaatttgc tggtttgcga acttcttaac
     2581 cgcaggttac cgcactaaat tgccgtaaac agcagaactt aggcactacg acaccgcgat
     2641 caagcccctg tttggaggca tctgaacacc aacgcgatcc tgcgcgggcg tattcgccat
     2701 gatgatccgt cgcgacgaac cccccaggat ctattgcgaa tgcagttcca agcccctgta
     2761 aatcctcatt agcattggcc gagaaaacgt gagcgatagc tttgcggacc ccgaacgggt
     2821 cgtgcatcga gaatagtgtg tgttgctcac catcgtgtgg gatcattggc atagcatgtt
     2881 ccgcgccggc taggccctca caggtttgga ctgcaatggg ggaaaatgtt ttgttcagcg
     2941 caagtccttc cgatctgaat gattgttttc gtgtacgctt gctacacccg ccggcgcggc
     3001 atcgttgcca cttctcgtgc atgtccgacc cccgaccagg cgccatagga acccacgaca
     3061 tacaggttct gcttcgcccg tgagacagcc acattcagga tgttcggcgt tcccgccgcc
     3121 cagcctctcg cgccgttctg cgatgcctta ggtgctccga gaacaaggat tacggtgtct
     3181 gcttcccgac cttgaaccgt atgaatggtc cccacgcgat ccgtcgtcca ctcacgaaca
     3241 tccgccttga atgcggcaaa caacgcagct tcacgcccca accgccgtcg cagttcctgg
     3301 gcgacgatct ggaatggcgt gatgatgaac agatcgggtt ctgtgatacc ggcagatgca
     3361 atcttcttga ggagatggac aaccaactca ccttccgccg gactccactt agagtctgcc
     3421 tcgccatcaa tattaagcca tgtagacggt ccaagcacgc ggccaatcgt tcctgcttcg
     3481 tgtgaacccg cggcgtagac catctggccg tcataggcga tccggttcga tattccgaac
     3541 attggctctc ggcagcggc
//
LOCUS       DASSHJ010000206        10718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1412636,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000206 DASSHJ010000000
VERSION     DASSHJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412636"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFI43_08025"
     CDS             complement(<1..366)
                     /locus_tag="VFI43_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7062110.1"
                     /translation="MNKRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQEHEV
                     HYVCADDTHGTPIMLRAEQEGITPKQLIDRVGKEHKADFDGFYVDFDSYYTTDSPENR
                     EFCNDIYRQLEANGLIEKRP"
     gene            584..1672
                     /gene="apbC"
                     /locus_tag="VFI43_08030"
     CDS             584..1672
                     /gene="apbC"
                     /locus_tag="VFI43_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HET7062111.1"
                     /translation="MAISEQQIQSALKEIVDLTTGKDYLSSNAARNIKIDNNDVSLAI
                     VVGYPARSVMESIRDQVTGKLKTIPGIGKVNVNITSRVAPHSVQRGVKLIPGVKNIIA
                     IASGKGGVGKSATAVNLALALAAEGASVGILDADIYGPSQPQMLGISGRPESPDGKSI
                     EPMKAHGIQAMSIGFLIDVETPMVWRGPMVTQALQQLLNDTRWDEVDYLIVDMPPGTG
                     DIQLTLAQKVPVTGAVIVTTPQDIALLDARKGLKMFEKVGIPILGIIENMSTHICSNC
                     GHEEHIFGEGGGEKMCKDYDVELLGSLPLDIHIREHIDTGKPTVVADPDGRVAELYRR
                     IARRLAVKIDELALDHTELFAKIVIEND"
     gene            1717..2283
                     /gene="dcd"
                     /locus_tag="VFI43_08035"
     CDS             1717..2283
                     /gene="dcd"
                     /locus_tag="VFI43_08035"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET7062112.1"
                     /translation="MTIKSDRWIRRMALEHGMIEPFESGQIKHANGHRIVSYGTSSYG
                     YDIRCSDEFKLFTNINSAIVDPKNFDSNSFVDVKSDVCLIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPFEPEWEGFVTLEFSNTTPLPAKIYANEGVAQVIFF
                     ESDEVCETSYKDRGGKYQGQHGVTLPKI"
     gene            2710..3885
                     /locus_tag="VFI43_08040"
     CDS             2710..3885
                     /locus_tag="VFI43_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HET7062113.1"
                     /translation="MQMRATALRQEVEAQEVFDTHPEVRLDFRHVQDVLKKGYTKPFL
                     LVDSDIVRNKARRFKSAMPRVHPHYAVKANPDPRVLKTLIEENVGFEIASISELDLLL
                     GLGVPAADIYYSNPIKSRAYIEYAAAKGVEWYVLDSVEELRKIVSVKPDAKLYVRIDT
                     PNIGSDWPLAGKFGTHMGEIKDIVHEAAKLHADLAGVTFHVGSQCRNPQNWRVGIERA
                     RKVFEDMRHAGLTPRLLNIGGGYPVRHVKPIPSIEVIGDVVNEAIADLPDDIRIMAEP
                     GRYLVSDAAYFVCRVEGTATRNGKRWMYWDAGVFGGLIEVTEGLRYEVLSDRKGAGIP
                     WAVAGPTCDSVDILMRDEILPEDIEEGDFIYIPNAGAYTTAYASNFNGFPLPDVVVL"
     gene            complement(3960..4481)
                     /locus_tag="VFI43_08045"
     CDS             complement(3960..4481)
                     /locus_tag="VFI43_08045"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7062114.1"
                     /translation="MTCRLSALVAVARNGIIGKDNALPWRLSADLKHFKALTMGHSII
                     MGRKTYESIGRLLPGRTSIIVTRQAGYKIENAIVVHSIEEALKACCGQDWNKAAGVEC
                     FVIGGAQIFREAAPNCDRMYITEIHRDFEGDVRFPEFNRKEWIEVSREKHQDDGGLEY
                     DFVVLDRKTNGPK"
     gene            complement(4478..5272)
                     /locus_tag="VFI43_08050"
     CDS             complement(4478..5272)
                     /locus_tag="VFI43_08050"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7062115.1"
                     /translation="MRQYLELMRHVLEHGHQKSDRTGIGTLSVFGYQMRFDLSEGFPL
                     VTTKKCHLKSIIYELLWFLRGDTNIKFLNAHGVTIWDEWADPNGDLGPIYGHQWRSWD
                     APGGKHIDQIAQAIGQIRKNPDSRRIIVSSWNVGELPEMRLPPCHALFQFYVSDGNLS
                     CQLYQRSADIFLGVPFNIASYALLTLMIGQVCGLKPGDFVHTFGDAHLYLNHIDQARE
                     QLSREPRKLPWMKLNPDVGNIFDFEYEDFTLEGYDPHPAIKAPVAV"
     gene            5402..6862
                     /locus_tag="VFI43_08055"
     CDS             5402..6862
                     /locus_tag="VFI43_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HET7062116.1"
                     /translation="MLPTLVQHSAQLAEPAMATHILIVLPKSNQTPDKLDIFGKKTLE
                     SILRRRKMQPEEISKAPVLGTLESGALCVWAMIDLARPVFDQQTSMRKAVQLLTEEKP
                     GEIHVAVYGNEEEKSSLVKVALYTAWINAAQLPSRKTGIKSAEAKPLAHIVLHGYKDL
                     QEVPLLKAKAEGNLLARDLTVLPPNELTPLNYRKRVQKLATAEGWKYTEFDVKALRKM
                     GAGAFVAVAQGSNTDDAAIVHLQRRIRNATKTVALVGKGICFDTGGYNVKPARHMHGM
                     NEDMNGSAVALGLLLAASRLDLPVNIDCWLAIAENLISPHAYKQDDVVTSLNGTTIEI
                     VHTDAEGRMVLADTLTLAAREKPDAMIDFATLTGSMHTALGSRYSGIFSNRADLLEKA
                     LAAGKSSGERTWSFPWDADYDAELESRVADVKQCTLEGEADHILATRFLSRFVDDAPW
                     LHMDLSASNNKGGLGAVASDVTGFGVSWGIDLLTQL"
     gene            complement(6892..7893)
                     /locus_tag="VFI43_08060"
     CDS             complement(6892..7893)
                     /locus_tag="VFI43_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7062117.1"
                     /translation="MKAILMKAPGSLDVLKLADVPKPELTDPYTVLVRLYAAGVNPID
                     TKVRKANAYYPDQLPSILGCDGAGVVEAIGSSVTRVRPGNDVFFFNNGLGGPPGTYAE
                     YAVVNEDYLALKPKNISMQEAAAIPVAMITAWEALLDRGNLRKGKHALIHAGAGGVGH
                     IAIQLARHFGARVATTVSGPAKAAFAQSLGTELAINYAEQDFADATLNWTNGLGADVV
                     LDTVGGETFCKSFAATKLYGRVVTLLSTGCDIRYTNTARLRNLIIGYVQITAPLVFGL
                     HSLRMEQTRILERGGELIERGLLKVHVSHALPLEQASEAHRLIEAGHTTGKIVLQID"
     gene            8012..9118
                     /gene="aroC"
                     /locus_tag="VFI43_08065"
     CDS             8012..9118
                     /gene="aroC"
                     /locus_tag="VFI43_08065"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7062118.1"
                     /translation="MSGNTFGKIFCVTSFGESHGPAIGCVVDGCPPGMELSEEDIQRD
                     LDRRKPGTSRHVTQRRESDTVEILSGVFEGKTTGTPIALLIRNEDQRSKDYTKIMDTF
                     RPGHADYVYWQKYGIRDYRGGGRSSARETAVRVAAAAIARKWLLIKYGVVIRGFMSQL
                     GPIELSFKDWEAVSRNPFFAPDPDVVPRLEEFMDRLRKSGDSVGARITVVAEGVPVGW
                     GEPVYDRLDAEIAYAMMSINAVKGVEIGAGFSAVAQKGTEHSDEITPDGFLSNNSGGI
                     LGGISTGQDIFVNIAVKPTSSIRLARRSIDKAGHPVMVETHGRHDPCVGIRAAPIAEA
                     MLALVLMDQALRHRAQNADVVCATPRIPARRLED"
     gene            complement(9216..>10718)
                     /locus_tag="VFI43_08070"
     CDS             complement(9216..>10718)
                     /locus_tag="VFI43_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7062119.1"
                     /translation="DQLRHEANVLCKDASVRHILWLHSRLTRHTAEDPAILSVGLDMT
                     EHKRAEGRLAWLADHDPLTDLFNRRRFQEELEQMLNLAARYKHSGALLFFDLDQFKYI
                     NDTSGHQAGDALLKMVARMLLGSIRSVDILGRLGGDEFAVILPQTTAEGAIEVAKNAL
                     ASLSKGQITVNGRTHKALASIGIALFPEHGNNVHDLLAAADLAMYQAKEAGRAGWHLF
                     SDEEKTRERMHTLVYWKEKIEHALLHERFLFYFQPIMHIPDKSINHYEVLLRMLDDDG
                     TVLAPNLFIPAAEQTGLIHAIDHMVLTKAIAQSAQIQHNGQRVHFSINLSAHAFRDPE
                     LLPTLTKAFDRYSADPTNFMFEITETAALEDLDAAKELMETIKALGCSFTLDDFGVGF
                     SSFYYIRQLPVDIVKIDGSFIRNLAENPDDQILVKALCDVARGFGKKTTAEFIETSAT
                     LSILESMNVDYAQGFLIGTPVSAEESPFRDYLKPRNPACPSPIFDPPRQF"
BASE COUNT         2454 a         2808 c         2982 g         2474 t
ORIGIN      
        1 cgggcgtttc tcgatcaggc cgtttgcctc aagctgccgg tagatgtcat tgcagaactc
       61 ccggttctcc ggtgaatccg tcgtgtaata gctgtcgaaa tcaacataaa acccgtcgaa
      121 atcggcttta tgttcctttc ccacgcgatc gatcaattgc ttgggggtaa tgccttcctg
      181 ttccgcacga agcatgatcg gagtgccgtg cgtatcgtcg gcacagacgt aatggacttc
      241 gtgctcctgc attttctgga aacgcaccca gatatccgtc tggatatatt ccacgaggtg
      301 gccgaggtgg atactgccat tcgcgtaggg aagagctgag gtgacgagaa ttttccgctt
      361 gttcatcaat ggtctatata aggggccaaa ttgggcgcca aacgtgattg taacaaactg
      421 ggagccacaa ctggcgaaac ggccgcaatt cgcacgatag cggcttacca ttagagcgcg
      481 cggcctcatc ctcccggtat gttaatatcc aatgttgttg tcggcatact tatcgccgga
      541 taagtatgcc ggacgaggaa tgaaacaaca gaaggagtgc aaagtggcta tctcggaaca
      601 gcaaattcaa tcggcgctca aggagattgt ggaccttacg accgggaagg attacctgag
      661 cagcaacgcg gcgcgcaaca tcaaaataga caacaatgac gtctctcttg ccattgtggt
      721 ggggtatcca gcaagaagcg tgatggaaag tatccgcgat caggtgacgg gcaagctcaa
      781 aaccattccc ggcattggga aggttaatgt caacatcacg agcagggtcg cgcctcacag
      841 cgtccagcgc ggggtaaagc tcatccccgg cgtgaagaac attattgcga tcgcgtccgg
      901 aaagggcggg gtggggaaat cggctactgc cgtcaacctg gccctggcat tggccgcgga
      961 gggcgcttcg gtcggcatcc tggacgcgga catctacggc ccgtcgcagc cgcaaatgct
     1021 cggaataagc ggacgccctg agtccccgga tggcaagagc atcgagccga tgaaggcgca
     1081 tggcatccag gcgatgtcga tcggtttcct cattgacgtg gaaacgccca tggtatggcg
     1141 cggccccatg gtgacacagg ccttgcagca actgttgaac gatacgcgct gggatgaggt
     1201 ggattacctt atcgtggata tgccccccgg aacgggggat attcaattga cgcttgcaca
     1261 gaaggttccc gtcactggcg ccgtgattgt cacgactccc caggacatcg cgctgctgga
     1321 tgctcgaaaa gggctcaaga tgttcgaaaa ggtcggcatt cccatcctgg ggatcataga
     1381 aaacatgagt acgcatatct gctcgaactg cggacatgaa gagcacattt tcggggaagg
     1441 aggcggcgaa aaaatgtgca aggattatga cgttgaactg ctcggcagcc tgcccctcga
     1501 cattcatatc cgcgagcata ttgacacggg taagcccacc gtagttgcgg atccggatgg
     1561 gagggtcgct gaactttacc gccggatcgc ccggcgtctt gccgtgaaaa tagatgagct
     1621 cgctctggac cacactgagc tgttcgcaaa gatcgtgatc gagaacgact gatccccccg
     1681 atgagcagca cgattcagcg tcttaagtat ttttagatga ccatcaaatc ggacaggtgg
     1741 atacgtcgga tggcgttgga acatgggatg atcgagcctt tcgagtccgg tcagatcaag
     1801 catgcgaatg ggcacaggat cgtgtcctac gggacgtcca gttacggata cgatattcgc
     1861 tgctccgacg agttcaagct cttcacgaac atcaactcgg cgatcgttga ccccaagaac
     1921 ttcgactcga actccttcgt cgacgtgaaa agcgatgtct gcctcatccc tcccaactct
     1981 ttcgccctgg cgcgcactgt tgaatatttc cgcattccaa gaaatgtctt gaccatttgc
     2041 ctcggaaagt caacctatgc ccggtgtgga attattgtaa atgtcacccc ctttgagccg
     2101 gagtgggagg gtttcgtgac cctcgagttc tcaaacacca ccccgcttcc cgccaagata
     2161 tatgccaatg aaggcgttgc ccaagtgatt ttcttcgagt ctgacgaagt gtgcgagacc
     2221 tcgtacaagg atcgtggcgg aaaataccag gggcagcacg gcgtcaccct tcccaagatc
     2281 tgattacatc cccgggttcc cgcggctgag gcaggcctgc tagcggagtc agtcggcctt
     2341 tgaacgccaa gagcagacaa aggtcaaagg tctctaaact ccgcttgtta tcgtgctttc
     2401 agaccccaca tcctgtgtca ggatatggag cggcatcccg agcgggaatg gcatgctggg
     2461 tgggtttttt gacgtcgtcc gggtggacga aatatttttt ttattgctgg gcaaaaacgc
     2521 ttgattcccg ctgaatgcgg tctataatcc gcataatttt tttcacgttc aaactgggaa
     2581 cggaggtcat catgaaggag gtcatcatga aggaggtcat catgaaaagc aaagcaaact
     2641 acttcgatac ggctgatctg gtcaatattc atattcccgt aacgtagcac ccctgcaagg
     2701 accactctca tgcaaatgcg cgcgaccgct ctacgccaag aagtagaggc ccaagaagtt
     2761 ttcgacaccc atcccgaagt ccgcctggat tttagacatg tccaggacgt cctgaaaaaa
     2821 ggctacacaa agccattcct gctggtagat agcgacatcg tgcgcaacaa ggcgcgtcgc
     2881 ttcaagtcgg ccatgccccg ggtccatcct cactatgccg tcaaggccaa ccccgatcct
     2941 cgcgtgctaa agaccctgat cgaggaaaac gtggggtttg aaatcgcttc catttccgag
     3001 ttggacttgt tgctcggcct gggcgtacct gctgccgata tttattacag caatccgatc
     3061 aaatcgaggg catatataga atatgctgcc gccaaaggcg tggaatggta cgtgctcgac
     3121 agcgtggaag agcttcgtaa aatcgtaagc gtcaagcctg atgcaaagct gtatgtgcgc
     3181 atcgatacgc caaatatcgg cagcgactgg ccgctcgcag gtaaatttgg aacgcatatg
     3241 ggcgagatca aggacatcgt tcatgaagcg gcgaaactcc acgctgacct cgcgggagtc
     3301 acttttcatg tcgggtccca gtgccgcaat cctcaaaatt ggcgggtggg aatcgagcgg
     3361 gccagaaagg ttttcgaaga catgcgccac gccgggttga cgcctcgcct gctgaacatt
     3421 ggcggcggct atcctgtgcg tcatgtcaaa cctattcctt cgatagaagt tattggcgat
     3481 gtcgtgaacg aagccattgc cgatctgccg gacgatatcc ggatcatggc ggagcctggg
     3541 cgttatctcg tatcggacgc agcttatttc gtatgtcgcg tggagggcac ggccacccgg
     3601 aacggcaagc gctggatgta ttgggacgca ggcgtgttcg gtggtttgat cgaagttacc
     3661 gagggtctcc gctacgaggt tctctcggat cggaagggtg cggggatacc atgggctgtt
     3721 gccgggccga cgtgtgattc tgtggatatc ctcatgcggg atgaaatatt gcctgaggat
     3781 atcgaggaag gcgattttat ctatattccg aacgcgggtg cctataccac ggcttacgcc
     3841 agcaacttca acggcttccc attgccggac gtggttgtgt tgtgatgtgc tgatgtaaga
     3901 cgtcgggcta taagccctgg atatcgatga acggcgggat gagaggttat cccgccgttt
     3961 tattttggac cattggtctt gcgatcaagc actacgaaat cgtattccag tccgccgtca
     4021 tcctgatgtt tttcacggga tacttcgatc cactccttgc ggttgaattc cgggaagcgc
     4081 acgtctccct caaaatctct gtgtatctcc gtgatataca ttctgtcgca gtttggagct
     4141 gcctcgcgaa atatctgggc cccgccaatt acgaaacatt ccacgcccgc tgccttgttc
     4201 cagtcctgac cacagcaggc ttttagcgct tcttcgatcg aatggaccac tatcgcattt
     4261 tcgattttgt atcccgcttg tcgtgtgacg ataatcgaag tgcgtccagg cagcagcctg
     4321 ccaatcgatt cataggtctt gcgtcccatg ataatggaat ggcccatcgt aagggccttg
     4381 aagtgtttga ggtcggcgga caaccgccag ggtaacgcat tatccttgcc aatgattcca
     4441 tttcgcgcaa ccgctaccag agcggatagg cggcaggtca tactgcgacc ggcgccttga
     4501 tcgcggggtg tggatcatag ccttccagcg tgaaatcttc atattcgaaa tcgaagatat
     4561 ttccgacgtc gggattcagt ttcatccacg gcaatttcct gggttcccgg ctcaattgct
     4621 cacgtgcctg gtcgatatgg ttgaggtaaa ggtgcgcgtc gccgaatgtg tgcacgaaat
     4681 ctccaggttt cagcccacaa acctggccga tcatcaatgt gagcaaggca taagatgcga
     4741 tgttgaaagg aacgccgagg aatatatctg cacttcgctg ataaagctgg caggagagat
     4801 ttccgtcgct gacataaaac tgaaaaagcg cgtggcatgg cggcagtctc atctctggaa
     4861 gctcacccac gttccaggac gacacgatta ttcgccgcga gtcggggttt ttcctgatct
     4921 gcccgatcgc ctgggcgatc tgatcaatgt gctttccgcc gggtgcgtcc catgaccgcc
     4981 attggtgccc atagatcggc cccaggtctc cattcgggtc cgcccattcg tcccagatgg
     5041 taactccgtg cgcgttaaga aactttatgt tggtatcgcc tctcaggaac cataagagtt
     5101 cgtatatgat ggatttcagg tggcattttt tcgtggtgac gagagggaag ccctcggaca
     5161 gatcgaatct catctggtac ccgaacactg aaagggtgcc gatacccgtt cgatcagatt
     5221 tttgatgtcc gtgctcaagc acgtgccgca tgagttcaag atattggcgc atggaatcaa
     5281 acgagaaagc ggcgcagcca ctcgatacga aggccaatac ctgccccgat acccagtggc
     5341 agagctaaat accctatact taccaatttt ttaacacttt ttgacttaag ggcagccgtc
     5401 aatgctccca acacttgtac agcactctgc gcagcttgcc gagccagcca tggctaccca
     5461 tatccttatt gtactgccga agtccaatca aacacccgac aaactggaca ttttcggcaa
     5521 gaaaacgctt gagtcgatat tgaggcgccg caagatgcag cccgaggaaa tcagcaaggc
     5581 acccgtcttg ggcactctgg aaagcggcgc cctgtgcgtt tgggccatga tcgatctggc
     5641 caggccggtg ttcgaccagc agacttccat gcgtaaagcg gtgcaattgc tcacggagga
     5701 aaagcctggc gaaattcacg tggcagttta cggaaatgaa gaggagaagt catcactggt
     5761 caaggtcgcc ctctatacag cgtggattaa tgccgctcag ttgccgtccc gcaaaaccgg
     5821 gataaaaagt gcggaggcga agccgcttgc ccatattgtg ctgcacggct ataaagattt
     5881 acaggaggtg ccactgttaa aggcaaaagc tgaaggaaac cttcttgcac gggatctgac
     5941 agttttacct cccaatgaac ttactccctt gaattatcgc aaacgtgtcc agaagttggc
     6001 gaccgccgaa ggctggaaat ataccgagtt tgatgtgaag gccctgcgga agatgggggc
     6061 aggggctttc gtcgcggtgg cccaaggcag caataccgat gatgcggcga tcgtgcattt
     6121 gcaacgccgg atcaggaatg caacgaaaac cgtggcgttg gtcggcaagg gcatttgctt
     6181 tgacacgggc gggtacaacg tgaagcccgc tcgccacatg cacggaatga acgaggatat
     6241 gaatggctca gccgtggcgt tgggactgct gcttgctgcc agccgtcttg acttgccggt
     6301 aaatatcgat tgctggctcg cgatcgccga gaatcttatc agccctcacg catacaagca
     6361 ggatgatgtc gttacatcgc taaacgggac gactatcgaa attgttcata cagatgccga
     6421 gggacgaatg gtgctggctg atacgttgac gctggcagcc agggagaaac cggatgccat
     6481 gatcgatttt gcaaccttga ccgggagtat gcatacggcg cttggatcgc ggtacagcgg
     6541 aatcttcagt aaccgcgccg atctgctgga aaaggcactg gcggcgggaa aatcaagcgg
     6601 cgaacgaacg tggtccttcc cgtgggatgc ggattacgat gctgagctgg aaagccgggt
     6661 tgccgacgtc aagcaatgta cgttagaggg tgaagcagac cacatcctgg cgacacggtt
     6721 tctaagcagg ttcgtcgacg acgcaccctg gttgcatatg gatctttccg ccagcaacaa
     6781 caagggcggt ttgggtgcgg tagcaagcga tgttaccggt ttcggtgttt cctggggaat
     6841 cgatctgctt acacagctct aaagagtttt cagttttctt ccgattcctg ctcaatctat
     6901 ctgcagcacg atcttgcctg ttgtatgccc ggcttcgatc agtcgatgcg cctcagaggc
     6961 ctgctccagg ggaagtgcat gactgacgtg aaccttgagc aaacctcgct cgatgagttc
     7021 gccgccgcgc tcgagtatcc gtgtctgttc catccgcaag gagtgcaagc caaatacaag
     7081 gggcgccgtg atctgcacgt acccgatgat caggttgcgt agccgggcgg tattcgtgta
     7141 gcggatgtcg catcccgtgg acagaagcgt tacaactcgt ccgtacagct tggttgccgc
     7201 aaaggatttg cagaaggttt cgcctcccac cgtatccagc acgacgtccg cgcccaagcc
     7261 gttcgtccag ttgagagttg catcagcaaa gtcctgttcc gcatagttga ttgccagctc
     7321 cgtgccaagg gattgcgcaa acgccgcttt agcagggccc gaaacagttg ttgcaacacg
     7381 tgccccgaag tgacgcgcaa gctgaattgc gatatggccg accccgccag caccggcgtg
     7441 aatcagcgca tgcttgcctt tccgcaaatt gccgcgatcc agtaaagctt cccatgcagt
     7501 aatcattgct acggggattg cggcggcttc ctgcatggat atgtttttcg gcttcagcgc
     7561 gagataatct tcgtttacca ccgcatattc ggcgtacgtt cccggtggcc cgcctaaccc
     7621 attgttgaaa aagaatacat cattgcccgg ccgcacccgc gtcaccgagc ttccaatcgc
     7681 ttccacgaca cctgctccat cacacccgag gatcgagggc agttgatccg ggtaataggc
     7741 atttgccttc cgcacttttg tatcaatggg gttcacgcca gccgcataca gcctcacgag
     7801 caccgtgtat gggtccgtca gttccggctt cggtacgtcg gcaagtttca atacatccag
     7861 gctgcccgga gccttcatga gtattgcctt catggctcgc ctttcgttct gatgtaatat
     7921 tttttactgc ttgtgttcac cgcctcagcc ctcgagaaga acctcgaacg gggttatgtt
     7981 aaacttgact ccttgaattt gcgagcaaaa aatgtctggg aatacctttg gtaaaatttt
     8041 ttgcgtcacc tcttttggtg agtcacacgg tccggccata ggttgtgtgg tcgacgggtg
     8101 tccgccggga atggaattat ccgaagaaga tatccagcgc gacctcgatc gccgcaaacc
     8161 cggcacttcc cgtcatgtga cacagcgtcg cgaatcggac acagtggaaa tactctccgg
     8221 agtgttcgaa ggcaagacga cggggacgcc aattgcattg ctgattcgca acgaagatca
     8281 gcgtagcaag gattacacca aaatcatgga cacgtttcgg cctggccacg cggattatgt
     8341 gtactggcag aaatacggga taagggatta tcgtggcggg ggccgttctt ccgctcgtga
     8401 aacggcagta agagttgctg cagcggctat cgcaaggaag tggttgctca tcaagtatgg
     8461 cgtagtgata cgcggcttca tgtctcagtt ggggcccatt gagctttctt tcaaggattg
     8521 ggaagcggtc agccgtaatc cgttctttgc tccggatcct gatgtggttc cgcgtcttga
     8581 agaattcatg gacagactgc gcaaatcagg agattcagtc ggagcccgga taaccgtggt
     8641 tgcggaaggc gtgccggtcg ggtggggtga gccggtttat gaccggctcg atgcagagat
     8701 tgcttacgca atgatgagta tcaacgcagt aaaaggtgtg gaaatcggcg caggattttc
     8761 cgccgttgcc cagaagggga ccgagcattc ggacgagatt accccggatg gatttttgag
     8821 caataattcg ggtggcatac ttggcggtat ttccactggc caggacatct ttgtgaacat
     8881 cgcggttaag ccgacatcca gtatccgtct tgcgaggcgg tccatagaca aggccgggca
     8941 cccggtgatg gttgaaacgc acggtcgtca tgacccctgc gtaggtatcc gcgcagctcc
     9001 tatcgccgaa gcaatgcttg cgctggtatt gatggatcag gcattgcgcc acagggcgca
     9061 gaatgcggac gtcgtctgcg caacaccccg aattccagct cgccgactcg aggattgata
     9121 gaagtggctc ctccttgacg gaagaatcga gttcccgcac ctgtttactg ctggttaatc
     9181 aattgccggg caaggctccg gggacgagtg ttggtttaaa actggcgagg cgggtcgaaa
     9241 atgggcgagg ggcaagcggg atttcggggt ttcagatagt ctctaaatgg cgattcctcc
     9301 gctgataccg gggttcctat caaaaatccc tgagcgtagt ccacgttcat ggattcaagt
     9361 atcgatagcg tagcggaggt ttcgatgaat tctgccgttg tcttcttacc gaaacctctt
     9421 gccacgtcgc acagggcttt aacgagaatc tgatcatcgg gattttccgc caggttgcga
     9481 ataaaagacc cgtcgatctt gactatgtca acgggaagct gccgtatgta atagaaggaa
     9541 gagaaaccta cgccaaaatc atcaagggta aaactgcatc ccagggcctt gattgtttcc
     9601 atgagctctt ttgccgcatc aagatcctcc aaggcggccg tctcggtaat ttcaaacatg
     9661 aaatttgtcg gatcagcgct ataacgatcg aatgctttgg tcagagtggg cagcaattcg
     9721 ggatcccgga acgcatgcgc cgacaaattg atggaaaaat ggactcgctg cccgttgtgc
     9781 tgtatttgcg ccgactgggc gatcgctttc gtaagcacca tatgatcgat tgcatgaata
     9841 agcccggtct gctcagcggc ggggataaac agattggggg caaggacagt cccatcatca
     9901 tcgagcatgc gcagcagcac ttcataatga tttatggatt tgtccggaat atgcatgatg
     9961 ggctgaaagt agaacaggaa ccgctcatgt aaaagcgcat gctcgatttt ttctttccag
    10021 tatacaagcg tatgcatccg ttcacgggtc ttctcttcat cggaaaacag gtgccagccg
    10081 gcccgtcccg cttccttcgc ctgatacatc gccaggtccg ctgcagccag caagtcgtgc
    10141 acgttgtttc catgctcggg aaatagcgca attccaatgc tggcgagcgc tttgtgggta
    10201 cggccattta ctgttatctg ccccttgctc agggaagcga gcgcgttctt cgcgacctct
    10261 attgcgccct ccgcagtggt ttgcggcagt attacagcga attcgtcacc tcccagccgt
    10321 cccagtatat cgacgctgcg gatactgccg agcagcatgc gcgccaccat cttcagaagc
    10381 gcgtcgccgg cctggtgacc gcttgtatca ttgatatatt tgaactgatc caggtcaaaa
    10441 aagagcagcg cgcccgaatg cttatagcgg gccgcgaggt tcagcatctg ctccagctcc
    10501 tcctggaagc gccggcggtt gaataaatcg gtaagcgggt cgtgatccgc cagccatgca
    10561 agccgccctt ccgcccgctt gtgctcggtc atgtcgagtc cgaccgataa tatcgccgga
    10621 tcctcggctg tgtggcgagt caggcgggaa tgaagccaca ggatatgccg aacagaggcg
    10681 tccttgcata gcacgttcgc ttcgtggcgt agttgatc
//
LOCUS       DASSHJ010000207        12518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1386784,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000207 DASSHJ010000000
VERSION     DASSHJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12518
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386784"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFI43_08075"
     CDS             complement(<1..293)
                     /locus_tag="VFI43_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET7062120.1"
                     /translation="MSGTIGERLISEGVISAAQLAQAMVRQKQEGGDLGQALMGLGFL
                     ASCQLRQFFETVPQVPVKIADTGLSEAFLTDLLLKAAYMESGAFTLQPLATTLS"
     gene            701..991
                     /gene="groES"
                     /locus_tag="VFI43_08080"
     CDS             701..991
                     /gene="groES"
                     /locus_tag="VFI43_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7062121.1"
                     /translation="MNIRPLHDRVIVKRLEEERKTASGIVIPDSAAEKPDQGEIVAVG
                     KGKVGDDGKVRPLEVRVGDKVLFGKYSGQTVKVSGEELLVMREEDIMGVVEG"
     gene            1041..2699
                     /gene="groL"
                     /locus_tag="VFI43_08085"
     CDS             1041..2699
                     /gene="groL"
                     /locus_tag="VFI43_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110680.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET7062122.1"
                     /translation="MASKEVKFGDSARHKMVTGVNILADAVKVTLGPKGRNVVLERSY
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVKE
                     GMKYVAAGMNPMDLKRGIDKAVGITVEELKKLSKPCTTGKEIAQVGSISANSDPEIGK
                     IIADAMEKVGKEGVITVEDGSGLQNELEVVEGMQFDRGYLSPYFINNPDRQIALLENP
                     FVLLHDKKISNIRDLLPVLEQVAKAGKPLLIIAEDVDGEALATLVVNNIRGILKTCAV
                     KAPGFGDRRKAMLEDIAILTGGTVIAEEVGLSLEKATLKDLGQAKRIEVGKEETTVID
                     GAGDPQVIQGRVKQIRGQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRTRSALQGIKGDNNDQDAGIKIVLRALEEP
                     LRQIVANCGDEPSVVINKVMEGSGNFGYNAATSEYGDMVAMGVLDPTKVTRYALQNAA
                     SVASLMLTTDALVAELPKEESGGGGMGGMGGGMGGMGGMGGMDM"
     gene            3080..3922
                     /gene="hpnD"
                     /locus_tag="VFI43_08090"
     CDS             3080..3922
                     /gene="hpnD"
                     /locus_tag="VFI43_08090"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET7062123.1"
                     /translation="MTPDQYCQEKAIQSGSSFYYSFLYLPLERRRAITALYAFCREVD
                     DVVDECSDPLVARAKLAWWRVEIGAIFAGGDARPSHPVARALASVAKPFNLTAERLNA
                     IIDGMEMDLDHSRYPDFDTLRRYCYHVASVVGLCSADIFGYRNPQTLSYAENLGLAFQ
                     LTNIVRDVGEDARRNRIYIPQDELARFGVTEDDILNARETPNFQRLVQFQIERAEHYY
                     DKAFSMLPGEDRKSQRPGIIMAAIYRTLLDEIKRDNCHVMQQRVSLTPVRKLWIAWKT
                     WMKG"
     gene            4083..4829
                     /locus_tag="VFI43_08095"
     CDS             4083..4829
                     /locus_tag="VFI43_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HET7062124.1"
                     /translation="MNDLNRYRPPVGLLKERVILITGAGQGIGRAAALAYASHGATVI
                     LHGRNVRKLERVYDEIEALAGPQPAIFPLDLEKAGDNDFALLAESIERQLGRLDGILH
                     NASLLPGLAPLETHTLEQWLTVLRVNLAAPSALTRACLPLLKASPDSRVVMTSETHGH
                     VPAAYWGAFAVAKAGIEVLVRIQAQEWEIMPNVRINAIIPGPVHSPQRSRTHPGEIKQ
                     NLKQPEDLMPVYLYLMGPDSNALSGEIVTC"
     gene            5009..5093
                     /locus_tag="VFI43_08100"
     tRNA            5009..5093
                     /locus_tag="VFI43_08100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5043..5045,aa:Leu,seq:tag)
     gene            5180..6484
                     /gene="tig"
                     /locus_tag="VFI43_08105"
     CDS             5180..6484
                     /gene="tig"
                     /locus_tag="VFI43_08105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7062125.1"
                     /translation="MEANVEELGALERRLDVSIPQEKIRTEVESRLKRLARTAKIHGF
                     RPGKVPLKIVQQQYGPQIRQEVIGDELKRNFGEVIREKNLRVAGYPRFEPKNIEENAS
                     AFEFSATFEVYPDIVLGDLGSARIERPIVSVAEGDVSRTIETLRKQRVHYEPVQRPAE
                     KGDQITIDYRGTIDGQEFAGSQAENFTLVLGQGRLLKDFEDALLGMSTGESRTFEVNF
                     PPDYHGKEAAGKTARFEVKLHEVKAPQLPELDAEFAKSLGIADGDIEKLRGEVQVNLE
                     REASNRVRARMKEQVMQCLLDTAKSEAPKALVEIERERLMEDARRDLAARGMNKNMPL
                     PPELFNERAERRVKLGLILAEVVKLHGLQPKPEQVRAIVEDIAQSYENPSEVVKWHYA
                     VPDRLNEAESLALEENVVTWVLEKATVEDKPVMFDELMGRAA"
     gene            6505..7116
                     /gene="clpP"
                     /locus_tag="VFI43_08110"
     CDS             6505..7116
                     /gene="clpP"
                     /locus_tag="VFI43_08110"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET7062126.1"
                     /translation="MEPRSLGLVPMVIETSGRGERAYDIYSRLLKERVIFLVGPVTEI
                     SANLIVAQLLFLESENADKDIHFYINSPGGLVSAGMAIYDTMQYIKPDVSTLCIGQAA
                     SMGSLLLAAGAKGKRFCLANSRVMIHQPLGGFQGQASDIEIHAREILFLKRRLSELLA
                     KHSGQSMETIERDTDRDNFMSGDEAVSYGLVDAVLTSRNQSAS"
     gene            7176..8453
                     /gene="clpX"
                     /locus_tag="VFI43_08115"
     CDS             7176..8453
                     /gene="clpX"
                     /locus_tag="VFI43_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7062127.1"
                     /translation="MSEKTGGEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIELCND
                     IIREEIQGAEAAKLTKSDLPVPHEICQILDQYVIGQEQAKKILSVAVYNHYKRLRTIN
                     KAGESDDIELAKSNILLIGPTGSGKTLLAQTLARLLDVPFVMADATTLTEAGYVGEDV
                     ENIIQKLLQKCNYDAEKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIE
                     GTVASVPPQGGRKHPNQEFVQVDTTNILFICGGAFDGLDKIIRARSEKGGIGFSASVR
                     TQDERKDFGVLLRGVEPEDLVKYGLIPEFVGRLPVVATLEELGEAALIQILTEPKNAL
                     VKQYQKMFSMEGGVDLEFREQALRAIAKKALARKTGARGLRSILENTLLDTMFDLPSL
                     ENVAKVVIDYGSVNGDIKPILIYSDKPKVAKSF"
     gene            8541..10952
                     /gene="lon"
                     /locus_tag="VFI43_08120"
     CDS             8541..10952
                     /gene="lon"
                     /locus_tag="VFI43_08120"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7062128.1"
                     /translation="MPSPVSDPNQLSLPLLPLRDVVVFPHMVIPLFVGRPKSIKALEV
                     AMESGKTILLVAQKFAAKDEPGPEDLYQVCSVANLLQMLKLPDGTVKVLVEGSRRARI
                     VKVTDDGSYFSGEAAMIPPDAADNHEIEAMRRATLAQFDQYVKLNKKIPPEVLTSLSG
                     IDEAGRLADTIAAHLPLKLEQKQEVLEIFDVPKRLEHLLGLLETELDILQVEKRIRGR
                     VKRQMEKSQRDYYLNEQVKAIQKELGEGEDGADLEEIDKKIKAAQMSKEGRAKAEAEL
                     KKLRLMSPMSAEATVVRNYIDALVALPWKKKSKISRDLHVAETVLEEDHYGLEKVKER
                     IVEYLAVQQRVDKLKAPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGGVRDEAEI
                     RGHRRTYIGSMPGKILQNMTKVGVKNPLFLLDEVDKMGMDFRGDPSSALLEVLDPEQN
                     NSFVDHYVEVEYDLSDVMFVATANTLNIPPALLDRMEVIRLSGYTEDEKLNIAIRYLL
                     PKQIKNHGLKENELTVSESALRDITRYYTREAGVRALEREISKICRKVVKALLLKRDQ
                     KKIVINGRNLDKYLGVRRYTYGVAEEKNQVGQVTGLAWTEVGGELLTIETVVLPGKGK
                     TITTGKLGEVMQESVQAALSVVRGRSRTLGISDDFYQKNDVHIHLPEGATPKDGPSAG
                     IGICTAMVSALTGIAARADVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIKTVLIPED
                     NVKDLTEIPETIKNRLDIHPVKWIDQVLDMALERKPEALPVVPPAVPTPVAVEGDASS
                     TVIKH"
     gene            11382..11654
                     /locus_tag="VFI43_08125"
     CDS             11382..11654
                     /locus_tag="VFI43_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7062129.1"
                     /translation="MNKSELIDAIAKSSDISKASAGNALDGALTAIKNALKKGQTVTL
                     VGFGTFRVGKRAARTGRNPRTGAAITIKEAKVPKFTAGKALKDAVN"
     gene            11668..11743
                     /locus_tag="VFI43_08130"
     tRNA            11668..11743
                     /locus_tag="VFI43_08130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11701..11703,aa:Val,seq:tac)
     gene            11768..11844
                     /locus_tag="VFI43_08135"
     tRNA            11768..11844
                     /locus_tag="VFI43_08135"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11802..11804,aa:Asp,seq:gtc)
     gene            11910..>12518
                     /locus_tag="VFI43_08140"
     CDS             11910..>12518
                     /locus_tag="VFI43_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7062130.1"
                     /translation="MFDFVHKKRRIVQMILGLATLPFLFWGIESYRNAGGDDSIAVAA
                     GEKISRQEFEQALRNQQENLRGTMGERFDETLMERPEVRAAVLDGLIQQRLLRQEADR
                     VGLAVSDPQLIEMIQNISAFQEDGKFSKKRYEELLRGQGMTPKAFEARVRQDLMRQQL
                     ISPYQEHGFVPASVAEKIMRLSEEKREISLAKIQPEQFLSRMK"
BASE COUNT         3065 a         3046 c         3700 g         2707 t
ORIGIN      
        1 gaaagtgtcg tggcgagtgg ttgcagggtg aatgcgcctg actccatata tgctgccttg
       61 agcagcaaat cggtgagaaa cgcttcgctc agcccggtat cggcaatttt tacgggcacc
      121 tgtggaacgg tctcgaagaa ctggcgcaac tggcaggatg caagaaatcc aagccccatc
      181 agcgcctggc cgagatcacc gccttcctgc ttttggcgca ccatcgcctg cgccagttgc
      241 gccgctgata ttacgccttc actaatgagg cgttcaccga tcgtgccgga catgagccgc
      301 tagtaatgcg tgggtcaaaa atttaaagcc gtgccgcggg gtccgccaca gaggaaaatg
      361 cgccaggagc ccagtcatca tctatgccgt tgtaaaggta aatccgcgaa gggcgactat
      421 actgataata aatattattc ttcgcagcaa aaatagctgg aaaagttaga ctggaaacgt
      481 ttcaactcca actcctctgg ttataacggc cgactttgcc cgcaagtgac ccctgcggcc
      541 gcttttttat ttttcattca cttgaaattc agagcgacag cacctattat tagcactcgt
      601 tggcgtagag tgctaacaat gtgaaatcca ttccgggaac tcggcgtggg tcgggtttcg
      661 gcaggttttt attggcttaa ttttcaacag gagaagaaac atgaacattc gtcccctaca
      721 cgaccgtgtc attgtcaagc gactggaaga agaacgcaag actgcgtccg gcatcgtcat
      781 tcctgacagt gccgcagaaa agcccgatca aggcgaaatt gtcgccgtgg gcaaaggcaa
      841 ggtcggtgat gatggaaaag tacgcccact ggaagtgaga gtcggtgaca aggtgctgtt
      901 cgggaaatat tccggacaaa cggtaaaggt atcgggagaa gaactgctcg tgatgcgtga
      961 agaagacatc atgggcgttg ttgaaggctg atcaagagac ggcctactca atattttcgg
     1021 caaattagga gaaagagatc atggcatcga aagaggttaa attcggcgat tcggctcgcc
     1081 acaagatggt tactggggtc aacattctgg ccgatgcggt caaggttaca ctcgggccaa
     1141 aaggccgcaa cgttgtgttg gagcgttctt acggcgcacc caccattacc aaggacggtg
     1201 tgtccgtcgc gaaggaaatc gagctgaaag acaagttcga gaacatgggc gcccaaatgg
     1261 tgaaggaagt ggctagcaag acttccgacg tggcgggcga tggcaccacc actgctactg
     1321 tgctggcgca ggcgatcgtc aaggaaggca tgaagtatgt ggccgccgga atgaacccca
     1381 tggacctgaa gcggggtatc gacaaagcag ttggcataac agtcgaagaa ttgaagaaac
     1441 tttccaagcc ttgtacgact ggaaaggaaa tcgcgcaagt gggcagcatc tccgccaatt
     1501 cagaccccga gattggaaag atcatcgccg atgcgatgga aaaagtcggc aaggaaggag
     1561 tcattacggt tgaagatggt tccgggttgc aaaatgaact tgaagtggtc gaggggatgc
     1621 agttcgaccg cggctacctt tcgccctatt tcatcaacaa ccccgaccgc cagatcgcgc
     1681 tgctggaaaa tccttttgtc cttttacatg acaagaagat ttcgaacata cgggacctgc
     1741 ttccggtgct cgagcaggtt gccaaggcag gcaagccgtt gctgatcatc gcggaagacg
     1801 tggacggcga agcgctggcg acgcttgtgg tcaataatat ccgcggcatc ctgaagacct
     1861 gcgcggtaaa ggcgccgggc tttggcgacc gccgcaaggc catgctggaa gacattgcca
     1921 tcctgaccgg tggaacggtc attgcggagg aagtgggtct ctcattggag aaagcgaccc
     1981 tgaaagacct tggtcaagcc aagcggatcg aagtcgggaa ggaagaaacg acggtgatcg
     2041 atggcgcagg cgatcctcag gttatccagg gcagagtcaa gcagatccgg ggtcagatcg
     2101 aggaagctac cagcgattat gacaaggaga aattgcagga gcgcgtggcg aagctggccg
     2161 gcggcgtggc cctgatcaag gtgggtgcgg cgaccgaagt tgaaatgaag gagaagaagg
     2221 cacgtgtgga agacgccttg catgcgaccc gggccgcggt cgaggaaggc attgtcccgg
     2281 ggggtggcgt agctttgata cgtacgcgct ctgcgctaca gggaatcaag ggtgacaaca
     2341 atgatcagga tgcaggcatc aagattgttc tgagagcatt ggaagagcct cttcgacaga
     2401 ttgtcgccaa ttgcggcgat gagccttctg tcgtcatcaa caaggtcatg gaaggcagcg
     2461 gcaattttgg atacaacgcc gcaacgagcg aatatggcga catggtcgcc atgggcgtcc
     2521 tggatcccac gaaggtgact cggtatgccc tgcagaatgc tgcttcggtc gcaagcctga
     2581 tgctcactac ggatgcgctc gttgccgaac tgcccaagga ggaatccggg ggtggcggca
     2641 tgggtggcat gggtggcggt atgggtggca tgggcggcat gggcggcatg gatatgtaag
     2701 aaccttgccc ggccatttcc ggtgctacat gaaaaccccg ctacggcggg gtttttgttt
     2761 gaaaaccatg gaggggatct cgaatcaagc cgcttcagac tttgttcaaa tgttccttaa
     2821 atatgttatg ttcccgcgtc gatccgccta ggggcgttgg tcgaagtaat tgcgggttac
     2881 gcgtgctgaa gggtgtgtcc ggtaaaactg ttaaaatagg gcgggaatgt gcgtgatgaa
     2941 ggattatccg cacgccttgc ttgtcgtcca taagccattt cggctagccg atttgaaaca
     3001 tgaggtccac gcgagcggta agggttcacg agtagaaaat gagcggcaag gcccacggtt
     3061 acaatcctgg atttccaaca tgaccccaga tcagtattgt caggaaaaag cgatacaaag
     3121 cggttcaagc ttctattaca gtttcctcta tctgcctttg gaaaggcgac gggctattac
     3181 tgcgctttac gcattctgcc gtgaagtgga cgatgtcgtg gatgaatgtt ccgacccgct
     3241 ggtggcgcgc gcaaagcttg cctggtggcg ggtggaaatc ggggccatct tcgctggcgg
     3301 ggatgcaagg cccagccacc ccgtagccag ggcactggct tccgtggcca agccgttcaa
     3361 tcttacggcc gaacggttga acgcaattat agatgggatg gaaatggatc ttgatcacag
     3421 caggtatcct gacttcgata cgctccgccg ctattgttac catgtggcca gcgtggttgg
     3481 attatgttct gcggatatat tcggatatcg aaatcctcag acgttaagtt acgctgaaaa
     3541 tctgggcctt gcattccagc tcaccaatat cgttcgtgat gtgggggagg atgcacggag
     3601 aaatcgtatt tatatccctc aggatgagct tgcacgcttt ggagttaccg aagacgatat
     3661 attgaacgct cgcgaaacac ccaattttca acgtctcgta cagtttcaaa tcgagcgggc
     3721 tgagcattat tatgacaagg ccttttcgat gcttccggga gaggaccgca aaagtcagcg
     3781 gcccggcatt atcatggcag ccatttaccg tactctgctt gacgaaataa agagagacaa
     3841 ctgccacgtc atgcaacagc gggtcagctt gacacctgta cgaaagcttt ggattgcctg
     3901 gaagacctgg atgaaaggtt aatcgattat cttattatct tatccagccc cgcttcgatt
     3961 tttccgtcga cttctatcga cttctattta cacaaaagtc catcgagcat gggatcctgc
     4021 tatccggata gtcaaggcgg ataggcgctt gcgcgtcttt tccatatgct cgttactcgt
     4081 caatgaatga tttgaatcgc tatcgcccgc cggtgggttt gctgaaggag cgcgtcatcc
     4141 tgattaccgg ggcagggcag ggtatagggc gagcggctgc gctggcgtac gcgagccatg
     4201 gcgcgaccgt tattctgcac gggcgcaatg tcaggaagct cgaaagggtc tacgacgaga
     4261 tcgaggcgct cgcaggtccg caacccgcaa tttttccgct tgatcttgaa aaggctggag
     4321 acaatgattt tgcgttgctc gctgagtcca tcgaaaggca gttgggcagg cttgatggca
     4381 tattgcataa cgcgtcgttg ctgccagggc ttgcgccgct tgaaacacac acgctggagc
     4441 aatggctgac agtgcttcgt gtcaacctgg ctgctccctc ggcgttgacc cgcgcgtgcc
     4501 tgccgctact gaaggcttcc cccgattccc gcgtcgtcat gacttccgaa acgcatggtc
     4561 atgttcccgc tgcatactgg ggagctttcg ccgtcgcgaa agcagggatc gaggtgcttg
     4621 tcaggataca ggcgcaggaa tgggaaatca tgcccaacgt tcgcatcaac gcgatcatac
     4681 cgggccccgt gcactcgccg cagcgatccc ggacacatcc gggcgagatc aagcaaaacc
     4741 tgaagcaacc ggaggatctc atgcctgtct acctgtacct gatgggtccg gacagcaacg
     4801 cgttgagcgg cgagatcgta acgtgctgat cctgcttcag ggatcttcgg acggcgctcg
     4861 tgccgtgtct ccctaccgtc agatcgcccg aaatttccat ctcgccggca caaaccatta
     4921 taaccatgct cagagcggcg ttgtccggat tcaggatggg ctgatgtagg gaggagaaac
     4981 gggtataatg gatttgcaca aaaacggcgc gaaagtggcg gaattggtag acgcaccaga
     5041 tttaggttct ggcgccgaaa ggtgtggggg ttcgagtccc ccctttcgca ccagttaaca
     5101 gtagcagtca ttgtgcaatt attccttcat aatcccgtgg cggaacgccg cacgatctta
     5161 tcaattgccg ggaaattcaa tggaagcaaa tgtggaagag ctgggggcgc tggagcgtcg
     5221 ccttgatgtt tctattcctc aagagaaaat caggaccgaa gtcgaaagtc gcctgaagcg
     5281 tttggcgcgg acagcgaaaa ttcacggttt tcgccctgga aaggttccgc tgaaaatcgt
     5341 tcagcagcaa tatggcccgc agatacgcca ggaggtgatc ggtgacgagc taaaaaggaa
     5401 cttcggcgag gttatacggg aaaagaacct gagagtggcg ggttatccac gctttgaacc
     5461 gaaaaatatt gaggagaatg cttctgcgtt tgaatttagc gctacgttcg aggtctatcc
     5521 cgacatagtg ctgggtgatc tgggttcagc ccggatcgag cggccaatag ttagcgtggc
     5581 cgagggggat gtatccagga cgatagagac gttgcgaaaa caacgggtcc attatgagcc
     5641 ggtgcaaagg ccggccgaaa aaggcgatca gatcacgatt gattatcgcg gtactatcga
     5701 cgggcaggag tttgcaggca gccaagccga gaattttacc cttgtgcttg ggcagggtag
     5761 attgctcaag gatttcgagg atgcgctcct cggaatgagt acgggtgaga gcagaacatt
     5821 tgaagttaac tttcctccgg actatcatgg taaggaggct gccggcaaaa cagcgcgctt
     5881 cgaggtgaag ttgcatgagg taaaagcacc gcagctgccc gaactcgatg cagaatttgc
     5941 gaagtcactg ggaattgcag acggcgatat cgaaaagttg cggggggaag tccaagtcaa
     6001 cctggagcgc gaagcatcga atagggtgag ggcacggatg aaggagcagg tcatgcagtg
     6061 cctgctcgac accgcgaaaa gcgaggcgcc caaagcgctg gtggagattg agcgggaacg
     6121 cctgatggag gacgcgcgtc gcgatctcgc tgcgcgaggc atgaacaaga atatgcccct
     6181 acccccggaa ctgtttaatg agcgtgcgga gcggcgcgta aaactcggct tgatcctggc
     6241 ggaagtcgtg aagcttcacg gcttgcaacc caaaccggaa caggtgcgtg ctattgtgga
     6301 agatatcgcg caaagctacg aaaacccgtc ggaagtggtc aagtggcatt acgcggtgcc
     6361 ggatcgcctg aacgaggcag aatctttagc gttggaagag aatgttgtaa cgtgggtgct
     6421 ggaaaaggct acggtcgaag acaagccagt gatgtttgac gaattgatgg gaagagcggc
     6481 atgattcagt ggaagcagga ggatatggaa ccccgcagcc ttgggctggt gcctatggtt
     6541 atcgaaacca gcgggcgagg cgagcgcgct tacgatattt attctcgctt gctgaaggag
     6601 cgtgtgatat ttctcgtggg gccggttacc gagatatccg ccaacctgat tgtggcgcaa
     6661 ctcctgttcc tggagtcgga gaacgccgac aaggatattc atttttacat caattcacct
     6721 ggcggcctgg tttctgccgg aatggcaatc tacgatacca tgcagtatat caagccggat
     6781 gtgagcacgc tctgcatcgg ccaggccgct agcatgggat cgttattgct ggctgccggg
     6841 gccaagggga aacgtttttg cctggcaaac tcgcgcgtca tgatacatca gcctttaggg
     6901 gggtttcagg gccaggcttc ggacatcgag atacatgcga gggaaatcct gtttctgaaa
     6961 cgacgcctga gcgagcttct ggcaaaacac agtggtcaaa gcatggagac gattgaacgg
     7021 gacacggatc gggacaactt catgagtgga gatgaggctg tcagctatgg tcttgtcgat
     7081 gctgtgctaa cctctcgaaa tcagagcgcg agttgaaata cgggggtatg aggttcacct
     7141 tcaggaacac ctgctcataa acgcgagata acactatgtc agagaaaacg ggtggcgaga
     7201 aactgcttta ttgctccttc tgtggcaaga gccagcatga ggtaaaaaag cttattgctg
     7261 gcccatcggt attcatttgt gatgaatgta tcgagctatg caacgatatc attcgtgagg
     7321 aaatacaagg agcggaagcg gcgaagctga cgaaatcgga tttgccggtt ccgcacgaga
     7381 tatgccagat tcttgatcag tatgtaatcg gccaggaaca ggcgaaaaag atcctgtccg
     7441 ttgctgttta caatcattac aagcgcttgc ggaccatcaa caaggcgggc gagtccgacg
     7501 acatcgagct ggcgaaaagc aacatccttc tgatcggccc tactggctcg ggaaagactc
     7561 tgctcgcgca gacgcttgcc cgtctgctcg atgtgccctt cgtcatggca gatgcgacaa
     7621 ccctcaccga agcgggttac gttggggagg atgtcgagaa tatcatccag aagcttttgc
     7681 aaaagtgtaa ttatgacgcg gaaaaggctc agcagggcat cgtctatatc gatgagatcg
     7741 acaagatatc gcgtaaatcg gacaatccgt ccattacgcg tgacgtttcc ggagaaggcg
     7801 tacagcaggc attgctgaag ctgatcgaag gaacagtggc atcggtgccg ccgcaagggg
     7861 gtagaaagca tccgaaccag gaatttgtgc aggtcgatac gaccaatatt ctcttcattt
     7921 gcggcggggc gtttgatgga cttgacaaga tcatcagggc ccgctcggaa aagggaggca
     7981 taggtttcag cgcaagcgtg aggactcagg atgagcgcaa ggacttcggc gtattgctgc
     8041 gcggggttga acccgaggat ctcgtcaaat acggcctcat ccctgaattc gtcggccgct
     8101 tgccggtggt cgcaacgctc gaagagttgg gtgaagcggc gctgatccag attttgaccg
     8161 agccgaagaa tgcccttgtg aagcagtacc agaaaatgtt cagcatggaa ggcggagtcg
     8221 acctcgaatt ccgcgaacag gcgctcaggg ctattgcaaa aaaggccttg gcgcgcaaga
     8281 ccggtgcccg cggattgcgc tcgattctgg aaaatacctt gctggacacc atgttcgatc
     8341 taccatccct ggaaaacgtc gccaaagtcg tgatcgatta cggctctgtc aatggcgaca
     8401 tcaaacctat cctgatctac tccgataagc cgaaggtggc taaaagcttt tagctcggat
     8461 ttcaggttgg ccgattctcc gtcttgattt tcagggcggc gtccccataa cttctgcttg
     8521 tctttttaca ggtgagtcaa atgccctctc cagtcagtga tcccaaccag ttatccttgc
     8581 cattgttgcc gcttcgggat gttgtcgttt tcccgcacat ggtcattccg ctctttgtcg
     8641 gccgtcccaa atccatcaag gcccttgaag tcgcgatgga gtctggcaag acgattttgc
     8701 tggtggctca aaaatttgca gcaaaggacg agccaggccc cgaagatctc taccaggtat
     8761 gcagcgttgc gaacctgctg caaatgctca agttgcccga tggcactgtc aaggtattgg
     8821 tggaaggcag ccgccgcgca cggattgtaa aggtgactga tgacgggagc tacttttccg
     8881 gtgaagcggc catgataccc cctgacgcag cggacaatca tgagatcgag gctatgcgcc
     8941 gcgcgacgct ggcgcaattc gatcaatacg taaagctcaa caagaagatt cctccagaag
     9001 ttctcacatc gctcagtgga attgatgaag caggccgtct cgcggatacc attgctgccc
     9061 atctgccgct caagcttgag caaaaacagg aagtgctgga aattttcgat gtgccgaagc
     9121 gcctggaaca cctgctcggc ttgctggaaa ccgaacttga catccttcag gtcgaaaagc
     9181 gcattcgtgg aagagtcaag cggcagatgg aaaaaagcca gcgcgactac taccttaacg
     9241 aacaggtgaa agccattcag aaggagttgg gggaaggcga agatggcgcc gatctcgaag
     9301 agatcgacaa gaaaatcaag gctgcccaga tgtccaagga aggacgggcg aaggcggaag
     9361 cagaactcaa gaaattacgc ctgatgtcgc ccatgtcggc tgaggccact gttgtacgta
     9421 actatatcga tgcgctggtt gcccttccct ggaagaagaa gagcaagatc agcagggatc
     9481 tgcatgtggc cgagacggta ctcgaagagg atcattatgg ccttgaaaag gtaaaggagc
     9541 gcatcgtcga gtacctggcg gtgcagcagc gtgtggataa gctgaaggcc ccgattcttt
     9601 gcctggttgg ccctccagga gtcggcaaga cttcactggg ccaatccatt gctcgcgcaa
     9661 cgaaccggaa attcgtgcgc atgtctcttg gtggcgttcg cgacgaggcc gagatccggg
     9721 ggcatcgccg cacctatatc ggatcgatgc cgggcaaaat tcttcagaac atgaccaagg
     9781 tgggggtcaa gaatccgtta ttcctgttgg acgaagttga caagatgggg atggacttcc
     9841 ggggcgaccc ctcctccgcg ctgctcgagg tgctggatcc cgaacagaat aattccttcg
     9901 tcgatcatta tgtcgaggtt gagtacgatt tatccgacgt gatgttcgtc gcaaccgcca
     9961 atacgctgaa cattcctcct gcgttgctgg accggatgga agtgatcaga ctgtcgggtt
    10021 ataccgagga tgagaagctc aatattgcaa tccggtacct tttacccaag cagataaaaa
    10081 accatgggct gaaggaaaac gaattgacgg tttctgaatc ggcgttgcgg gacattaccc
    10141 gatattacac ccgggaggct ggcgtccggg ccctggagcg cgagatttcg aagatatgcc
    10201 gcaaggtggt gaaagccctt ctgctgaaac gcgatcaaaa gaagattgtc atcaacggcc
    10261 gaaatctcga taaatatctg ggcgtgcgac gttacacgta tggcgtggcg gaggagaaga
    10321 accaggtggg acaggttacc ggcttggcct ggacagaggt gggcggtgag ttgttgacga
    10381 tcgagacagt tgtgctcccc ggcaagggaa agaccattac gaccggaaag ctcggtgaag
    10441 tgatgcagga gtctgtccag gcggcgctgt cggtagttcg cggccgatcg cggacgttag
    10501 gaatttccga tgatttttac cagaagaacg atgtacatat ccatttgccc gagggtgcga
    10561 cgccaaagga cggaccgagc gcgggtattg gaatttgtac ggccatggta tcggcgctaa
    10621 cgggcatcgc cgcgcgagca gacgtcgcca tgacaggtga aatcacgctg cgcggagagg
    10681 tgctgcctat cggtgggttg aaggaaaagc tgcttgccgc gcatcggggt ggaataaaaa
    10741 cggtgctgat tcccgaggat aatgtcaagg atctgacgga aattccggag accatcaaaa
    10801 accgtctcga tattcatcct gtcaagtgga tagaccaggt gctggatatg gcactggagc
    10861 gcaaaccaga agcgcttccc gtcgttcctc cagcagtgcc taccccggta gcggtggaag
    10921 gggacgcgtc aagtacagtc atcaagcact agctttgctc cgggaacctc tgatccagag
    10981 gttcccgagg ttttcctgga cttgagcccg gaataaaatt cagtgcggcc tcatgtcctt
    11041 tcaaggggag cctgagctgc ttcagcgtat ttccaggtga taatccatcg tcttcaacaa
    11101 ttagcgggag cggcagcaga ccaacgcttc ttctctgccc atgcaggcgt ccgcgctgtt
    11161 tgcctcgcgg ctcatgctat agcggtttac gaggtacccg agagcaaaaa ctgagcttat
    11221 ggtggctatg ccccgttttg cacggcaatt ggagagcgtc ctgagctcta tagacgtctt
    11281 ttaaatattt ccacaatcgc ttgaccaagc acaagtggct tgatataaaa ccaattgcag
    11341 ggtttttgca gctctctcaa caataacgaa aggggaatca cgtgaataaa tctgaactga
    11401 tcgatgccat cgcaaagtct tccgacattt ccaaagcatc tgccggcaat gctctggacg
    11461 gagcattgac cgcaataaaa aacgcattaa aaaaaggtca aaccgtaacg ctggtcggat
    11521 ttggaacttt cagggtaggc aagcgagcag cgcgcaccgg tcgtaatccc cgcaccggcg
    11581 cagcgatcac gattaaagaa gcaaaagttc ccaagtttac tgctggcaag gcgctcaagg
    11641 atgcagtaaa ctaacgggtt tttgacgggg tgcttagctc agttggtaga gcgtcgccct
    11701 tacaaggcga atgtcggcgg ttcgaccccg tcagcaccca ccatcagtcg ggcaggttga
    11761 atttgctgga gtggtagttc agttggttag aataccggcc tgtcacgccg ggggtcgcgg
    11821 gttcgagtcc cgtccactcc gccagctttt cacgttgaac aggcgaacgc aagttcgcct
    11881 tttttttatt cgctctccga atctctacaa tgtttgattt tgttcataag aaaagacgca
    11941 tcgtccagat gatccttggc ctggcgacgc tgccgttcct gttctggggc attgaatcgt
    12001 accgcaatgc cgggggggac gactctattg ccgtggccgc gggagagaag atttcgcggc
    12061 aggagttcga gcaggctctc cgtaatcagc aggaaaacct gcggggaacg atgggcgaga
    12121 ggtttgacga aaccctgatg gaacggccag aagtgcgcgc cgcagtgctg gacgggttga
    12181 tccagcagcg cctgttaagg caggaggcgg accgcgtcgg attggccgtc tctgatccgc
    12241 agttgataga aatgattcag aacatctcgg ctttccagga agacgggaaa ttctccaaga
    12301 aacgatacga ggagttgcta aggggccagg ggatgacgcc aaaagctttc gaagcgcgcg
    12361 tccggcagga cctcatgcgc cagcagctca tttcccctta tcaggaacac ggttttgttc
    12421 ctgcatccgt tgcagagaag atcatgcgat tgagtgagga gaaacgcgag atcagcctgg
    12481 ctaaaataca gcccgagcaa tttttatccc ggatgaaa
//
LOCUS       DASSHJ010000208         6052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_38404, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000208 DASSHJ010000000
VERSION     DASSHJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38404"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFI43_08145"
     CDS             <1..487
                     /locus_tag="VFI43_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7062131.1"
                     /translation="GGPLTEKKVKKVTLILPDGTEYGKEGELNFAASQIDPALGTQQL
                     RATFDNSEHQLLPGQFVRARVTTGERSGVFLVPQPAVQTSDLGKSVYVVNSKNEATVR
                     PVVVGHWIGTNWVILDGLKAGDKVIVDNIIKLRPGTPVAPHAPGENPPPPASPAEEKQ
                     A"
     gene            496..3648
                     /locus_tag="VFI43_08150"
     CDS             496..3648
                     /locus_tag="VFI43_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET7062132.1"
                     /translation="MGSSFFITRPIFSSVISIIIVLAGLAAAMQLPIAQYPQIAPPTV
                     LVTATYPGASAETLTKTVAAPIEEQLSGVENLLYFSSVADSSGTLTITVTFDVGTDID
                     QATFNVSNRVNIALPRLPDEVRRTGLIIQKRSNDILLVFMLISKEKGKYDPLYLSNYA
                     TLNLLDELKRTKGVGDAIVFGGQDYSMRIWLRPDRMAQLGVTTTDIATAIQAQNAQYA
                     AGKIGQEPAPADQQLVFTVNAKGRLLEPEQFGNIILRAEGPEGVLHLRDVARIELGAQ
                     SYDVRTALNGQPGAGIPIFLQPGANALETAEALMAKMDELKQRFPPGMDYVVPYDTSK
                     FVKASMWEVVKTLGEAMVLVIVVVYLFLQSWRATLIPIIAVPISLIGTFAGLWVFGFS
                     INTLTLFAMVLSIGIVVDDAIVVLENIERLMDEEKLSPLNAAIRSMQQVARAVVAIVL
                     VLCAVFVPVAFMGGIAGELYRQFAVTVAVAVVISGMVALTLTPALCAILLKHTHGESA
                     FFRAFNGGFGRLTHFYTSMVDTTIRHKIIGAVVFTGIIGISIYLIETVPGSFVPPEDQ
                     GYVVTATILPDGATLARTTRTAEAVRAAIAQDPAVAHQFVVNGFDLIGGGNKTSSATM
                     FVAFKDWSERTETAEDIVNKLMGIGMQQSDGIAIAFNPPSIRGLGTAGGFEVYVQSRA
                     GGSPLQLAEVMNNFIAALNQEPRLAGIATFFRPTVPQFFIEVDEPKAISQGVRIADIY
                     ATLQSTMGSLYINDFNRLGRTYRVQLQAEPQYRMKPEDLGRVYVRSQSGAMIPLSALS
                     KISTVVGAEQLERYNGLLSAKILGGGAPGVSSGETIELVEEIAEKNLPSNYQIAWTGQ
                     AFQEKRTGSAAVFAFSFAIIMVFLILAAQFETWALPLAVIMAVPFALAGALLAVLVRG
                     MPNDIYFQVGLITLIGLAAKNAILIVEFATQKMKEGMPVAQAAVEAARLRFRPIVMTS
                     MAFVLGILPLVFATGAGAAARRSMGTGVLGGMLLATFVATVFIPLFFTWLSRKRIQRP
                     DENVRREKLEGTHDLAPEEM"
     gene            3689..5107
                     /locus_tag="VFI43_08155"
     CDS             3689..5107
                     /locus_tag="VFI43_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET7062133.1"
                     /translation="MLLPGVLLSSLLLASCAMGPDYSRPAIDVSDKFRMGETEGQSIA
                     NLPWWELINDEQLQLLIRQALLGNKNLKQAVASVEELQARLGVARMDFLPKLDMNVNA
                     PAFGRMGGFLFPGFPTPYSYFGQATLNWEIDIWGRVRRSNEAALADLMAREENRRAVV
                     LTLVSNVAQSYFDLLQFDMQLTIARNALRSWEESVALSQSQLRGGTISRLDLDQFNAE
                     RANAATRVAELERMMVQKENELSVLLGNNPVTVARGRSLTEQVIPPEVPAGLPSELLQ
                     RRPDILQAEKTLHAATARIGMAKASRFPRLTITGFLGVASPALSKILLSGSEFGVGGL
                     GLAGPLLNAQSLGLEQRAAEAQARQVLAQYEQSVLVAFKEVEDALVAVRTSADQRRAQ
                     QEQVEALRSALNISNLRYKGGITSYVDVLLAKRNLFDAEFALASTHRLQLASVIQLYK
                     ALGGGWAPGMGMTSTSFPGAGG"
     gene            5276..>6052
                     /locus_tag="VFI43_08160"
     CDS             5276..>6052
                     /locus_tag="VFI43_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7062134.1"
                     /translation="MPQNRGVGYMDVTGQSTNPTVHNNPYVSSLRAPALGLAVPLFLA
                     PILLLGCSQKAPQSPPQQIPEVEVINVITQTVPDEPEFIGRTEAFRPVEIRPQVTGII
                     QAVYFTEGRNIHKGEKLYLIDPVPFKAVYLNTEARVAQARARLIQAEQNLARVRPLLE
                     AQAVSQKDVDDAVAEMLAAKAALEGARSDLVRAKFDFDNTLITAPVSGRIGRSNFYEG
                     RLVSAQTALLTTIDQLDPMYVNVSVPESYLLRRRRELAEHK"
BASE COUNT         1296 a         1718 c         1757 g         1281 t
ORIGIN      
        1 tggcggaccc ctgaccgaga aaaaagtaaa aaaagtaacg cttatactgc ctgacggcac
       61 ggaatacggg aaggaaggcg aattgaactt cgccgcgagc cagatcgatc ctgcgctggg
      121 aacgcagcaa ttgcgagcaa cgttcgataa cagcgagcac cagttgctgc cgggtcaatt
      181 tgtccgcgcg cgggtaacga ccggcgagcg cagcggcgtc ttcctcgtgc cgcagcctgc
      241 ggtgcaaaca tcggacctgg gtaaaagcgt gtatgtcgtc aacagcaaga acgaagcgac
      301 ggtgcgtccc gtcgtggttg gccactggat tggtacaaac tgggtcatcc ttgatggact
      361 gaaagccggc gacaaggtca tcgtcgacaa catcatcaaa cttcgtcctg gaacacctgt
      421 tgcacctcat gctccggggg agaatcctcc tccgcctgct tcgcccgctg aggaaaagca
      481 ggcttagagg acccaatggg cagttcattt ttcattacgc ggccgatttt ctcgtcggtc
      541 atatccatca tcatcgtctt ggcgggactg gccgcagcaa tgcagctacc catcgcccaa
      601 tatccgcaga tcgcgccccc cacggtgctg gtcacagcca cctatcctgg cgccagcgcg
      661 gaaactctga caaaaaccgt tgccgctccc attgaagagc agttgagcgg agtggaaaat
      721 ctcctctact tcagttcggt ggcggattca agcggtacct tgaccatcac cgtcaccttc
      781 gacgtaggca cggatattga ccaggcaact ttcaacgtca gcaaccgcgt caacatcgcg
      841 ctgccgcgcc tccccgatga ggtacggcgc acgggattga tcatacagaa gcgctccaat
      901 gacattctcc tcgtcttcat gctcatctcg aaggagaagg ggaaatacga cccgctgtat
      961 ctaagcaatt acgccaccct caacctgctc gacgaactca agcgaaccaa gggtgtcggc
     1021 gatgcgatcg tatttggcgg acaggactat tcgatgcgta tctggttgcg cccggaccgg
     1081 atggcgcagc tcggcgtgac aacaacggat attgccacag caatccaggc gcagaatgcg
     1141 caatatgcgg cgggcaagat cgggcaggag ccggcgcctg cggatcagca actggttttc
     1201 accgtcaatg cgaaggggcg cctgctggag cccgagcaat tcggaaatat catcctgcgc
     1261 gccgaggggc ccgaaggcgt cctccacctt cgggatgtcg cccggatcga actaggagcg
     1321 caaagctatg atgtacgcac cgcgctgaat gggcagccgg gcgctggtat ccccatcttt
     1381 ctgcagcccg gggccaacgc gcttgaaacg gcggaagcgc tcatggcgaa gatggatgag
     1441 ctgaagcagc gtttcccccc ggggatggat tatgtcgtgc cttacgatac cagcaagttc
     1501 gtcaaggcat cgatgtggga agtggtcaag acgttgggcg aggcgatggt actggtcatt
     1561 gtggtggtat acctgttcct gcaaagctgg cgcgcgactc tcattccgat cattgcagta
     1621 cccatttccc tgatcggtac ttttgccggc ttgtgggtat ttggtttttc gatcaataca
     1681 ctcacccttt tcgcgatggt tctgtccatc ggcattgtcg tggacgatgc aattgtcgtg
     1741 ctggaaaata tcgagcgcct gatggacgag gagaaactgt caccgctcaa cgctgccatt
     1801 cgctcgatgc agcaggtcgc gcgcgccgtt gttgccatcg tgctggtgct ctgcgccgtg
     1861 ttcgtgccgg tggcattcat gggcggcatt gcgggcgagc tctaccgcca gttcgcggta
     1921 accgtggcgg tcgcagtcgt catttccggc atggtggcgc ttacgctcac tccagcatta
     1981 tgcgcgatcc tgctgaagca cactcacggg gaatccgcgt tctttcgtgc attcaacggg
     2041 ggatttgggc gcctgaccca tttttatacc tcgatggtgg acacgaccat tcgtcacaaa
     2101 atcattggag ccgtcgtatt caccggcatt atcggcattt cgatctatct gatcgaaaca
     2161 gttccaggca gcttcgtgcc gccggaagac cagggttacg tggtcacagc caccattctt
     2221 cctgacggcg cgacgctcgc gcggacaacc cggacagctg aagcggtgcg tgctgcgata
     2281 gcgcaagacc cggccgttgc gcatcagttc gtggtcaacg gctttgacct catcggagga
     2341 gggaacaaga cgagttcggc gacgatgttc gtcgcgttca aggattggtc ggagcgcacg
     2401 gaaaccgccg aggatattgt aaataaactg atgggtatcg ggatgcagca atcggatgga
     2461 atcgcgattg cattcaaccc gccctcgatt cgaggcctgg gtacggcggg gggatttgaa
     2521 gtgtatgtgc aaagccgggc cggtggcagc cctctccagc ttgcagaggt catgaataat
     2581 ttcatcgccg cgctgaacca ggaaccgcgc ctggccggca tcgcgacttt ctttcgacca
     2641 accgtgccgc agttcttcat cgaggtggat gagcccaagg ctatttccca aggggtgaga
     2701 atcgctgata tctatgcgac cttgcaaagc acgatgggat cgctttatat caacgatttc
     2761 aaccgccttg gacgcaccta ccgggttcag ctccaggccg agccgcaata tcgcatgaag
     2821 ccggaagatc tgggcagggt ctatgttcgc tctcagtcgg gagcaatgat tccgctttcc
     2881 gccttgagca aaataagcac ggttgtcggg gctgaacaac tggaacggta caacggcttg
     2941 ctttccgcaa aaatcctcgg cggaggcgca ccgggcgtga gctccggcga aaccatagag
     3001 ctcgtggaag aaattgcgga aaaaaacctg cccagcaatt atcaaattgc ctggaccggg
     3061 caggctttcc aggaaaaacg cacgggttct gcggccgtat tcgcgttcag tttcgcaatc
     3121 atcatggtat tcctcatcct tgcagcgcaa ttcgaaacct gggcgctacc cctggctgtc
     3181 atcatggcgg taccgttcgc gttggccggg gcgctgctcg ccgtgctggt aagggggatg
     3241 ccgaacgata tttatttcca ggtgggattg attacactta tagggcttgc cgcaaaaaat
     3301 gccatcctta tcgtagagtt cgccacccaa aaaatgaagg aaggcatgcc tgttgcacaa
     3361 gcagcggtcg aagctgcacg tctgcgcttc cgcccgattg tcatgacttc gatggcattc
     3421 gtgctaggca tcctgccgct cgtattcgca acaggcgcgg gtgcagcggc acggcgctca
     3481 atgggaacgg gagtgttggg tggaatgctt ctcgccacat tcgtcgctac tgttttcatt
     3541 cccttgtttt ttacctggct gagccgcaag cgcatacaac gaccggatga aaacgtgcga
     3601 agggaaaagc tggagggaac tcatgatctt gctcctgaag aaatgtagca gcgcctggga
     3661 ggcgcgctgt tccagagcca gagcgcttat gttgctcccc ggcgtgttgc tctccagtct
     3721 cctgctcgcc tcgtgcgcaa tggggcccga ttattcccgg cccgctatcg atgtctcgga
     3781 taaattccgc atgggggaaa cggaggggca atccattgcc aatcttccgt ggtgggagct
     3841 tattaacgac gagcagcttc aactcctgat caggcaggcg ctcctgggaa acaagaattt
     3901 gaagcaggcg gtcgcaagcg tggaagaact ccaggcgcgt ttgggcgtcg cccggatgga
     3961 tttccttccc aaattggata tgaatgtcaa tgcacccgct ttcggccgca tgggcggttt
     4021 tcttttcccg gggtttccga ctccctacag ctattttggc caggccacgc tgaattggga
     4081 gatcgatatc tggggcaggg ttcgacgttc gaacgaagcc gcactcgccg atctcatggc
     4141 gcgagaggaa aaccgccggg cggtcgtgct gaccctggtc agcaacgtgg cgcaatccta
     4201 cttcgatctg ctgcagttcg acatgcagct taccattgcc cgcaacgcgc tccgctcgtg
     4261 ggaagaatcc gttgcgctct cccagtcgca acttcgcgga ggcaccatat cgcgcctcga
     4321 cctggatcaa tttaacgctg agcgagccaa tgcagccaca agggtggcgg aacttgagcg
     4381 gatgatggtg caaaaggaaa acgagttgag cgtcctgttg ggaaacaatc ccgtaacagt
     4441 cgcccgcggc cgctctctga ctgaacaggt gatcccccct gaagtgcccg ccggtcttcc
     4501 ttcggaattg ctgcaacgcc gtcccgacat actgcaggct gagaaaacac tgcatgcggc
     4561 aacggctaga ataggaatgg ccaaagcctc ccgctttccc aggctcacca ttaccggttt
     4621 tctcggcgtt gcgagtccgg ccttgtcaaa aatattgctc tccggaagcg agttcggcgt
     4681 tggaggactg ggattggcgg gtccattgct caacgcccaa agcttgggtc tggagcagcg
     4741 cgcggcggag gctcaggcaa ggcaggtgtt ggcgcaatac gagcaatctg tcctggtcgc
     4801 gttcaaggaa gtcgaagacg ctcttgtagc tgttcgcaca tctgccgacc agcgcagggc
     4861 gcagcaagag caggttgaag cgctgcgctc agcgctgaac atatcgaatc tgcgttacaa
     4921 gggcggcatt accagctatg tggatgtgct tctcgccaag cgaaatctgt tcgacgccga
     4981 attcgcgctc gcctccacgc accgcctcca gctcgcgtcg gttatccagc tctacaaggc
     5041 tctgggcggt ggatgggctc cgggaatggg aatgacaagc acttcattcc cgggggcagg
     5101 cggctaagcg aattcgagtc tcttccccgc tccagcttat ctgcaacccg ccttcccctc
     5161 gctttcacgg gcatttccaa ggagcaggca atctctcccc aatcgcccaa gcaagaccgg
     5221 caagagcatt tgcggtcgag acatgattgc cctaatctga aatcgggcac tctttttgcc
     5281 acaaaaccgg ggggtcgggt atatggatgt aacagggcaa tcgacgaacc ccactgttca
     5341 caataatccc tacgtttcct ccctgcgcgc tcccgcgctt ggtctggccg tgcctttatt
     5401 cctggctccg attctacttc tgggttgctc gcagaaagcg ccccaaagcc caccccagca
     5461 gatcccggag gtggaagtca ttaacgtgat tacgcagacg gttcctgacg agccggaatt
     5521 tatcggaagg actgaagcct tccgcccggt agaaattcgg ccccaggtta cgggaattat
     5581 tcaggcggtc tacttcacgg aggggcgcaa tatccacaag ggggaaaaac tgtatctcat
     5641 cgatccggtt cccttcaagg ctgtctacct taacaccgag gcgagagtgg cgcaggctag
     5701 agcgcggttg atccaggcgg aacagaacct ggcccgagtg cggcctcttc tggaagcaca
     5761 agcggtgagc cagaaagacg tagacgatgc ggtggccgaa atgctggcag cgaaggctgc
     5821 cctggaaggc gcgcgaagcg acctggtcag ggcaaaattt gatttcgata ataccctgat
     5881 caccgcgccg gtgagcggac ggataggtcg cagtaatttt tatgagggca ggttggtctc
     5941 cgcccagacc gctcttctga ccaccatcga tcaactggat ccgatgtatg tgaacgtgag
     6001 cgtgcctgaa agctatcttc tgcggcgccg ccgcgaactc gccgagcaca ag
//
LOCUS       DASSHJ010000209        15022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_918880, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000209 DASSHJ010000000
VERSION     DASSHJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 15022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15022
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918880"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFI43_08165"
     CDS             <1..221
                     /locus_tag="VFI43_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7062135.1"
                     /translation="VGLVVFILSVVIQYGLAHVPANQAVVIMLFELVVASIAAYFFAD
                     EAMTLREWAGGAMIVSASLFSAKINRKQ"
     gene            340..1290
                     /locus_tag="VFI43_08170"
     CDS             340..1290
                     /locus_tag="VFI43_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET7062136.1"
                     /translation="MSHHIVFLERDSVKAHIRRPKFEHTYEEYPHTADDQVVERLRPA
                     TIAIINKVQLSEETLAQLPRLQLVAVSATGYNVIDIDACRRRGIDVVNIRNYAIHTVP
                     EHVFALVLALKRNLLAYRQEIEQGAWQKSEQFCLFTHRIRELRGSTMGIIGEGAIGRG
                     TAALARGFGMKVLFAYHPSSKKKDVILTPFDQVLAESDVLSLHCPLTPATRNMIGIDE
                     LRKMKRDALLINTARGGLVDEAALVQALDEGQIAGAGFDVLTTEPPVAGNPLLDARRP
                     NFILTPHVAWASDEAMQVLADQLIDNIEAWAAGRPQNLVT"
     gene            complement(1315..3234)
                     /locus_tag="VFI43_08175"
     CDS             complement(1315..3234)
                     /locus_tag="VFI43_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HET7062137.1"
                     /translation="MQPACLVVALLCWFCLFPVTAHSKSNPASDYLAELQQQVRQKKL
                     AHERVWHLLLKYDKRPFVGTVSEADGMEFFNSPHGKTDSEAELAATLASFFVPLSEIP
                     EGKEHPQCNFPARFKWLKQQLVFDLARLEIQTCDRLGRWMDTLDPGGATLVFASYFMN
                     NPASMFGHTLLRIDSRRTGTGNNLLNYGANYAAIPDTDNAFLYALRGLIGSFKGRFAI
                     FPYYTKVQEYNNWESRDLWEYELKFSPDQLNMLLLHLWELGGTYFDYYYFQENCSYHV
                     LSLLEVANPDLRLKDSFFFSVIPTDTVKTVMAQEGLVKEIVYRPAVVNQMNQKRLRMT
                     GDEKRILQDMIKGDLSVESPAFGSLPQPSQALLLNAYMDYLQYRSMQEKSDKEIKIPR
                     SVLLARSRLQDAGDKFEGIVKRFSSAPHEGHGTDRFRLAVGHNSHEPFLEFAYRPAYH
                     DLMARAAGYDKDSEIIFMDFKLRYYAESDRLRLDQAKFLSITSLNPYDPLFPKSSWRA
                     DLGIDTLRDRTCGYCNSLKGSYGRGVSYRPDFFSPLLLYSFLDLKAEVSDHLRQNYRL
                     GGDAEVGMYYDITENLRVKFAGSYDVFVLGESKRFFSAHFITRYALSRDLDVRVEWNR
                     YDHNNEGIFSANYFF"
     gene            complement(3302..3811)
                     /locus_tag="VFI43_08180"
     CDS             complement(3302..3811)
                     /locus_tag="VFI43_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HET7062138.1"
                     /translation="MGRYSRIAVAASLFLLPVSVFAAGYGPAGCGLGSMVVGSKPGGE
                     QVLAATTNGTFGSQTFGITFGTSNCGDNGLVNLTKEREVFAQENYTSLIKEMAQGKGE
                     NLNTLASLYQCPASSQQEFGAMVQEKFDSLVMSDQTTSTELLSHLEKQLNRHPTLAKS
                     CNTQVSALD"
     gene            4329..4748
                     /locus_tag="VFI43_08185"
     CDS             4329..4748
                     /locus_tag="VFI43_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HET7062139.1"
                     /translation="MELKGSKTEGNLKAAFAGESQANRRYLYFANKADVEGQNDVAVV
                     FRSTAEGETGHAHGHLEYLEASGDPATGLPIGASRENLKAAIAGETHEYTDMYPGMAK
                     SAREEGFDEIADWFETLAKAERSHANRFQKALDSLTD"
     gene            4888..6243
                     /locus_tag="VFI43_08190"
     CDS             4888..6243
                     /locus_tag="VFI43_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET7062140.1"
                     /translation="MTTREGSLEAPKRHPLDWENPDFYDEPKLFTEMERVFDICHGCR
                     RCVNLCTAFPRLFDLIDDGPTGELDGVDKHKFWEVADRCYLCDMCSMTKCPYVPPHPW
                     NVDFPHLMLRAKAVKHKKGDVRFRDTLLSSTDAVGKLASIPVVAQTVNAMNKSPAVRK
                     LMDTTLGIHAERKLPEYASHKFRGKARPNAQHEVRDGARTRGKVAIYATCYVNYNEPG
                     IGHDLLKVLDHNGIPVRLVEKEACCGMPKLELGDLAAVKELKEKNIPPLARLVKEGYA
                     ILTAVPSCTLMYKQELPLMFPNDEDVKAVAEAMFDPFEYLMLRNRDGLLKTEFTKPLG
                     NVSYHIPCHLRVQNIGQKTRELLQMIPGTKINTVERCSGHDGTWGVKSEHFAESMKIG
                     KPVFRQMATQGPDYISSDCAIAARHIEQGMGEQGKGGSPGKKAQKEHPLTLLRIAYGL
                     E"
     gene            6297..6899
                     /locus_tag="VFI43_08195"
     CDS             6297..6899
                     /locus_tag="VFI43_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HET7062141.1"
                     /translation="MDRVTHDSLLTLEAYAKARQEFRAHVMAHKKNRTIHLGKNVTLI
                     FEDELTVRYQIQEMLRAEKIFDENGIQDELEAYNPLIPDGTNWKATMLVEFPDPVERA
                     ARLRELIGIEDRVWVKIEGFDPVHAIADEDMERETEEKTSSVHFLRFELSPAMIDALH
                     NGASLSMGIAHSSYDASCDPVQSGVRDSLINDLVATDGAS"
     gene            6896..7426
                     /locus_tag="VFI43_08200"
     CDS             6896..7426
                     /locus_tag="VFI43_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HET7062142.1"
                     /translation="MMKRLFLLLCWIPVLVPIGAYAEKTMDYEFDNEKPWAEIQAQLP
                     PYPKTENLLQFDAGPASTNSHYVDISSLSLGEDGVVRFSLVIKSASGAMNVSYEGIRC
                     QTAEKKTYAYGRNNNTWLEARASRWTDLENIAQNYAQRTLYRYFFCPLGIDMVRDADE
                     AIQALKAGVNPRAVRY"
     gene            complement(7469..7798)
                     /locus_tag="VFI43_08205"
     CDS             complement(7469..7798)
                     /locus_tag="VFI43_08205"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HET7062143.1"
                     /translation="MKITEIDGNFSITPQISAENLNEIKAAGFKSVICNRPDSEDGGA
                     HPGHELMESEARKLGLEFAYLPVIPGQINDNHVDQFRALVDKLPGPVLGYCRMGMRAK
                     TLYERSR"
     gene            complement(7876..8994)
                     /gene="proB"
                     /locus_tag="VFI43_08210"
     CDS             complement(7876..8994)
                     /gene="proB"
                     /locus_tag="VFI43_08210"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET7062144.1"
                     /translation="MQSIVKQSRRIVIKVGSSLVTNQGQGLDHGALACWAEQIARLKE
                     VGKEVVLVSSGAIAEGMQRLGWKRRPQAVHELQAAAAVGQMGLVQAYESCFRAHDLHA
                     AQVLLTHEDLSDRKRYLNARSTLTTLLSMNVIPVINENDTVATDEIRFGDNDTLAALV
                     TNLIEADVLVILTDQGGLFTADPRKDTKATLVREARAGDAAIEAMAGGAGSSIGRGGM
                     LTKVLAAKRAARSGAHTVIASGHEPDVLMQLAHGTAIGTQLLAETMTLAARKQWLADQ
                     LQMRGNITLDEGAVEALTSGGKSLLSIGVTNVSGNFERGEVVGCLDPHGNEIARGLVN
                     YSAAETRRILRRTSNEIEAVLGYLGEPELIHRDNMVLL"
     gene            complement(8999..10030)
                     /gene="obgE"
                     /locus_tag="VFI43_08215"
     CDS             complement(8999..10030)
                     /gene="obgE"
                     /locus_tag="VFI43_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7062145.1"
                     /translation="MKYIDEAMIEVIAGKGGDGAAAFRREKYIPRGGPSGGDGGRGGS
                     IFAVADRNINTLVDYRFARIFRAKNGENGRGSDCYGKSAEDITLRMPVGTVITNIATG
                     EVVADLERDGEKVLLAKGGTGGLGNLHFKSSTNRTPRQFTPGEPGEELELKLELKVLA
                     DVGLLGMPNAGKSTLIRAISAARPKVADYPFTTLHPNLGVVRVDENRSFVVADIPGLI
                     EGAAEGAGLGYRFLKHLSRTRLLLHVVDVAPYDQAVDPVREAKAIVEELRKYDEALYE
                     KPRWLVLNKVDLLPEQERGKTCRNFIQNLGWKGKSFAISAITGEGCKELTYAIMEYLE
                     QEASKASED"
     gene            complement(10110..10367)
                     /gene="rpmA"
                     /locus_tag="VFI43_08220"
     CDS             complement(10110..10367)
                     /gene="rpmA"
                     /locus_tag="VFI43_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7062146.1"
                     /translation="MAHKKAGGSSRNGRDSNSKRLGVKSFGGELIPAGSIIVRQRGTR
                     FHAGNNVGMGKDHTLFAKATGTVNFGFKGSEKRKTVSIIPA"
     gene            complement(10377..10688)
                     /gene="rplU"
                     /locus_tag="VFI43_08225"
     CDS             complement(10377..10688)
                     /gene="rplU"
                     /locus_tag="VFI43_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7062147.1"
                     /translation="MYAIIKTGGKQYRVENGLRLKIEQIPADIGSELVIDQVLMIADG
                     ENVSMGKPILSGAAVKATVLQQGRHDKVRIFKMRRRKHYQKHQGHRQNYTEIQITGIS
                     A"
     gene            10901..11869
                     /gene="ispB"
                     /locus_tag="VFI43_08230"
     CDS             10901..11869
                     /gene="ispB"
                     /locus_tag="VFI43_08230"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HET7062148.1"
                     /translation="MSIERIRSLIANDMAAVDSVIREKLYSDVVLIRQVSEYIINSGG
                     KRLRPALVILSAGAFGYSGKYHHDLAAIVEFIHTATLLHDDVVDASELRRSRATANAL
                     FGNAASVLVGDFLYSRAFQMMVEVDNMRVMRVLADATNTIAEGEVLQLLNVRDPDVDE
                     ENYLRVIRYKTAKLFEAATRLGAILGNASAAEEEAMAVYGMHLGTAFQLTDDMLDYSG
                     DYQETGKNLGDDLAEGKPTLPLIFAMRTGSSAQAAIIRNAIAQGGEGGFQPVLEVIQQ
                     TGALAYARRRAQAESDTASKTISLLPDSDYKECLLQLCTFAVARNY"
     gene            12019..12095
                     /locus_tag="VFI43_08235"
     tRNA            12019..12095
                     /locus_tag="VFI43_08235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12053..12055,aa:Pro,seq:cgg)
     gene            complement(12290..12526)
                     /locus_tag="VFI43_08240"
     CDS             complement(12290..12526)
                     /locus_tag="VFI43_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062149.1"
                     /translation="MKVILLIARTYATCNTLSAAPSPTRGSGENRQYPTTTLCPQIVF
                     FMPVLFLDAGESLRENQVSWRLFAFYQLHQPLWT"
     gene            12580..12819
                     /locus_tag="VFI43_08245"
     CDS             12580..12819
                     /locus_tag="VFI43_08245"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HET7062150.1"
                     /translation="MGRILFLILVAVLFFLLIRSFARSLRKRREEIEKAQRSLEGEDM
                     VRCVHCGVHLPRSESVVSEGKFFCSDEHRRLHLQP"
     gene            12844..13407
                     /gene="ampD"
                     /locus_tag="VFI43_08250"
     CDS             12844..13407
                     /gene="ampD"
                     /locus_tag="VFI43_08250"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HET7062151.1"
                     /translation="MAEFDETGIDGEGYLGNTRFIPSPNFDERPEECGVNLLVIHNIS
                     VPPDDFGGEGVIELFTNVLDPEAHPYYQRLRGLKVSAHFFIRRRGEIIQFVSCEKRAW
                     HAGESCWQGRGRCNDYSIGIELEGSDCMPFTNAQYAALASLTHELQERYPIHDIVGHS
                     DIAPGRKTDPGPCFDWARYRTAISHRR"
     gene            complement(13463..>15022)
                     /locus_tag="VFI43_08255"
     CDS             complement(13463..>15022)
                     /locus_tag="VFI43_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HET7062152.1"
                     /translation="GKSTFMKILGGDLEPSSGNVAVDAGERVGKLRQDQFAFEDCVVM
                     DTVIMGHAELWQVKQERDAIYANPDATEADYMHAAELEARFAELDGYSAEARAGELLL
                     GVGIPLSQHQSMMSAIAPGFKLRVLLAQALFADPDILLLDEPTNNLDINTIRWLEDVI
                     NQSRSTIIIISHDRHFLNSVCTHMADLDYGEIRIYPGNYDEYMIASTQVRERMLADNA
                     KKKTQIAELQAFVSRFSANASKARQATSRARQIEKIKLEDVKPSSRQYPYIRIEVDER
                     EKLHRLAVSIQSISKQFPGMEKPLFHKFSMNVEAGEKIAIIGPNGIGKTTLLRCIAGD
                     LVPDSGEVKWAEKARSGYFAQDHSTDFENDVTLTDWMTQWRRENDDDQAVRSILGRLL
                     FSGDEVKKSVQVISGGEEGRMLFGKLMLQRNNVLLMDEPTNHLDMESIESLNSALEKY
                     TGTLIFVSHDREFVSSLATRIIEMEPEGITDFKGSYEDYLQGQGIGAFPVPARGNTAS
                     VEKEKRRASAR"
BASE COUNT         3432 a         3989 c         3976 g         3625 t
ORIGIN      
        1 tggtcgggct cgtcgtattt atcctgagcg tggtcatcca gtatggcctc gcgcatgtgc
       61 cggccaacca ggctgtcgtg atcatgctgt tcgaattggt cgttgcttcg atcgcggctt
      121 atttttttgc ggacgaggcg atgaccttga gggaatgggc gggtggagcg atgattgttt
      181 ccgcaagcct gttttcggcg aaaataaacc gcaagcagta gtctcacagc aggtccggct
      241 tttcgagcac ccccggattt tacggtggga gtgcttgagc ggtttgaggg tgctgagggt
      301 gctgaggtcg aaaaaagaac agtaataaga gggcatatta tgtctcatca tatcgttttt
      361 ctggaacgcg attcagtcaa agcacatata cgcagaccga aattcgagca tacctacgag
      421 gaatatccgc atactgctga tgatcaggtt gtggagcgac tgcgcccagc aacaattgca
      481 atcatcaata aggtgcagtt gagcgaggaa actcttgcac agttaccccg gctgcaacta
      541 gtggccgttt cggcgacggg gtacaacgtc attgacattg acgcgtgccg ccgccgaggg
      601 attgatgtcg tcaatatccg caattacgcg atccacaccg tgccggagca tgtgttcgca
      661 ctggtgcttg cactgaagcg gaacctgttg gcctatcggc aggaaatcga gcagggggcg
      721 tggcagaaat cagagcaatt ctgcctgttt acccatcgca tccgcgagct gcgtggatca
      781 accatgggga tcatcggaga gggtgcaatt ggtcggggca cggcagccct tgcgcgaggt
      841 tttggcatga aggtattgtt tgcataccat ccatcatcga aaaagaagga tgtcattctc
      901 accccgtttg accaggttct tgccgaatcg gatgttctct cgttacattg ccccctgact
      961 ccggcaacta gaaacatgat tggtatcgat gaattgcgca aaatgaagcg cgacgcgcta
     1021 ttgatcaata ccgcccgggg tggtctggtc gatgaagcgg cgctggtaca ggccctcgac
     1081 gaaggtcaga ttgccggcgc gggtttcgat gtgctcacca ccgagccgcc ggtagcgggt
     1141 aatccgctgc ttgatgcgcg ccgtccgaac ttcatcttga ccccgcacgt ggcctgggct
     1201 tcagacgagg caatgcaggt tctcgctgac cagttgatcg ataatatcga agcgtgggcc
     1261 gcgggcaggc ctcagaacct ggtcacatag aagctttagg tgggagcctg ggactcagaa
     1321 gaagtaattg gctgaaaaaa taccttcgtt attgtgatcg tagcgattcc attcgactct
     1381 cacgtcaaga tctcgggaga gcgcataacg ggtaataaaa tgagcagaga agaaacgttt
     1441 gctttcgccc aagacaaaaa catcgtaact gccggcgaat ttgactctca aattttccgt
     1501 tatatcgtaa tacatgccga cctccgcgtc tccgcccagg cggtaattct gccttaaatg
     1561 atcagaaact tccgccttca ggtcgaggaa cgaatacagc aggagcggcg agaaaaaatc
     1621 cggccgatag gaaacgcccc ttccataact gcccttcaag gaattgcagt agccacacgt
     1681 gcggtctctc agcgtgtcga ttcccagatc tgcgcgccag gaagacttcg gaaagagggg
     1741 atcgtaggga ttgagcgatg taatggaaag gaattttgcc tgatcgagcc tcaggcggtc
     1801 ggactccgca taataacgga gcttgaagtc cataaaaatg atttccgagt ctttatcgta
     1861 tcctgcggct ctggccatca aatcatggta agcggggcga taggcgaatt cgaggaatgg
     1921 ctcgtgagaa ttatgcccta ccgctaatcg aaagcgatcc gtgccatggc cctcgtgggg
     1981 ggcgctcgag aagcgcttga cgatgccttc gaacttatcg cccgcgtctt gcaggcgact
     2041 tcttgccaat aataccgagc gggggatttt tatttccttg tcactttttt cctgcatgct
     2101 tcgatactgc aaatagtcca tgtaggcatt caacagcagc gcttgtgatg gttggggaag
     2161 gctgccgaaa gcggggctct ccaccgagag gtcccctttg atcatgtctt gtaatatccg
     2221 cttctcgtct cctgtcatcc tgagccgttt ctgattcatc tggttgacca ccgcggggcg
     2281 atacacgatc tcttttacca gcccttcctg tgccatcacc gtttttacgg tgtcggtcgg
     2341 gattacacta aagaaaaagg agtcttttaa cctgagatcc ggattggcga cctccagcag
     2401 cgaaagcaca tgataagagc agttttcctg aaaataataa taatcaaaat aggttcctcc
     2461 gagttcccat aagtgcaaca gcagcatgtt caactgatcc gggctaaact tgagttcata
     2521 ctcccaaagg tcgcggcttt cccaattgtt gtattcctgc accttggtgt aataagggaa
     2581 aatggcgaag cggcctttaa atgagcctat caaacccctt agcgcataca gaaaagcatt
     2641 atccgtatcg ggaatcgctg cgtagtttgc gccgtagttc aacagattgt ttccagtgcc
     2701 cgtcctcctg ctatcgatgc gaagcagggt atgcccaaac atcgatgcag gattgttcat
     2761 gaaataagat gcgaatacga gggtggcgcc gccggggtcc aaagtatcca tccagcggcc
     2821 gagccgatca caggtctgga tttccaggcg ggcgagatcg aaaaccaatt gctgcttcag
     2881 ccacttgaat cgcgccggaa agttgcactg ggggtgttcc ttgccctctg gaatctcgct
     2941 caggggaaca aagaagctgg cgagggtggc tgccagctct gcttccgaat cggtttttcc
     3001 gtgcggagaa ttgaagaact ccattccatc ggcttcactg accgtgccga caaaaggtct
     3061 tttgtcgtac tttaaaagca gatgccagac tcgttcatgg gcaagctttt tttgcctgac
     3121 ttgctgttgc agttcagcca gataatccga agcggggttg gatttggaat gagcagttac
     3181 agggaaaagg caaaaccagc aaagtaacgc aactacgagg caagcaggct gcacaggtga
     3241 accatggagt gaacgatatc gacacggata cgggctttcg cccgtatccg gttactattt
     3301 tttaatctaa cgcggatact tgcgtattac aggattttgc aagtgtggga tgacgattta
     3361 actgcttctc gagatgactt agcaattcgg ttgaagtggt ctggtcactc attaccaagc
     3421 tgtcaaattt ctcctggacc atcgcgccaa attcctgctg gctggatgcc gggcactggt
     3481 acaagctggc aagggtattc aaattttcac ctttgccttg cgccatttcc ttgatcaggc
     3541 tcgtgtaatt ttcctgggcg aacacttcac gctctttggt caggtttacc aaaccattgt
     3601 caccgcaatt tgaagtcccg aatgtaattc cgaaagtttg agacccgaat gtcccgttcg
     3661 ttgttgcagc aaggacctgt tccccgcctg gtttcgatcc gacaaccatt gaacccagtc
     3721 cgcaacctgc tggcccatat ccagcggcaa aaaccgaaac gggaagtaaa aacagagaag
     3781 ctgctactgc aatccggcta tatcgtccca taattctctc cctataaaga aggttactat
     3841 caattcaaat ctggatcgat gggtctggta actcaccgat aatttgaaag aacttaattt
     3901 ttaaatgctt cttaaccact tcaatgatct ggaaaaaatt aatattcttc ttctccagac
     3961 gagattgaat ttactgaacg tttgctcacc tgtcaaatag ttattcatat ctcggctgca
     4021 tacagccggc gtggcgatcc gcttgcttga gcatttccca tactggcttc ttctggcctg
     4081 tttttgggaa attttgccaa aggaggccat gttcgatagc gctgagcggc tcgcatgtca
     4141 ccgtggctcg cccgggcatg ctctccgggc aatagttgac aagccatgca cccttcgcta
     4201 gtctcctacg gttcggtttt cgcctgtccc ctcaggcagg cgattcctgt gatcgagtcc
     4261 atacctcaag tattctgaat tttagtttgc agttagctat aacattaacc acggaacagg
     4321 agagactgat ggaacttaag ggatcaaaaa cggaaggaaa tctgaaagcc gcttttgccg
     4381 gggaatccca ggcaaaccgc cgctacctct actttgcaaa caaggcggat gtggaagggc
     4441 agaacgatgt cgcggtggta tttcgctcca ctgccgaggg cgagactggc cacgcgcacg
     4501 ggcaccttga atacctcgaa gcgagcggtg atccggcgac gggcctgcct atcggggcgt
     4561 cgagggaaaa tttaaaagca gccattgccg gcgaaactca tgaatacacc gacatgtatc
     4621 ccggcatggc caagtctgcg cgggaagaag gctttgatga gatcgccgac tggttcgaaa
     4681 ccctggcgaa ggctgagcgt tctcacgcca accgctttca gaaagccctg gacagcctga
     4741 ctgactgatt acccatatct gaaggatatt ctggaccgct tgccggtcca gaacatccgc
     4801 gctctttgct tcaaaacttc tcgccccgct tcttgtctac tgcttccacg aagtgtttga
     4861 tgttgaattc gtctatccga catttgcatg accacccgag aaggtagcct cgaggctcca
     4921 aagcgtcatc cgctcgattg ggaaaatccc gatttttacg acgagcccaa gctgtttacc
     4981 gagatggagc gcgtctttga catttgtcat ggctgcaggc gctgcgtcaa cctgtgcact
     5041 gcttttcccc ggctgttcga tttgatcgat gacggaccga ctggcgagct ggacggggtc
     5101 gataaacaca aattctggga agtagcagat cgctgttatc tctgcgatat gtgctccatg
     5161 acaaaatgcc cttacgtacc accgcatccg tggaacgtcg attttccgca tttgatgctg
     5221 cgtgccaagg ctgtcaagca taagaagggt gacgtccgtt tcagagacac cttgctttcc
     5281 agtaccgacg cagtgggcaa gctggcgagc attcccgtgg ttgcacaaac agtcaatgcc
     5341 atgaacaagt ctccagcggt ccgcaagctg atggatacta cgctgggcat tcatgccgag
     5401 cgcaagctgc ccgaatatgc cagccacaaa ttccggggca aagcgcggcc aaacgctcag
     5461 cacgaggtcc gtgacggtgc ccggacacgg ggcaaggtgg cgatctacgc aacatgctac
     5521 gtcaattaca atgaacccgg tatcggacat gacttgctga aagttctcga ccacaacggg
     5581 attccggtgc gcttggtgga aaaagaggct tgctgcggga tgccaaagct ggaactgggt
     5641 gatcttgccg ccgtgaagga actgaaagaa aaaaacattc ctcccctggc aaggcttgta
     5701 aaagaaggat atgcgatatt gacggctgtg ccgtcatgca cgctgatgta caagcaggaa
     5761 ttgcctctca tgtttcccaa tgatgaggac gtcaaagcag ttgccgaagc gatgttcgat
     5821 ccgttcgagt acctcatgct gaggaaccgg gacggcctgt tgaaaacgga atttacgaag
     5881 cctctcggca atgtttccta ccatattccc tgtcatttgc gggtgcagaa catcggccag
     5941 aagacgcgtg agttgctgca aatgattcca gggacgaaaa taaacacagt ggagcggtgt
     6001 tccggccacg atgggacttg gggtgtcaaa tccgagcatt tcgcggaatc catgaaaatc
     6061 ggaaagcccg ttttcaggca aatggcaacc cagggtccgg attacatcag ctcggattgc
     6121 gccattgcgg cgcgccatat cgaacagggt atgggtgaac agggaaaggg cggtagtcct
     6181 ggtaaaaagg cgcagaaaga gcaccccctg acgctcctgc ggattgccta cgggctggag
     6241 tagctcgaat aagcgataag cgtcgtaaaa aaattatcca ccaagcgagc tgaaacatgg
     6301 atcgagttac ccacgatagc ctgctgacac tggaggctta tgccaaggca aggcaggaat
     6361 tccgtgcgca cgtcatggcg cacaaaaaaa accgcacgat ccaccttgga aaaaatgtaa
     6421 cactcatatt tgaggatgaa ttgacggtcc ggtatcagat ccaggaaatg ctccgggcgg
     6481 aaaagatttt tgatgaaaac ggcatacagg acgagctgga ggcctacaac ccgctcattc
     6541 ccgatggcac caactggaag gccaccatgc tggtcgaatt ccccgatccc gtcgagcgcg
     6601 ccgcaaggct gcgggaactg atcggaatag aagacagggt atgggtgaaa atagaaggct
     6661 tcgacccggt tcatgcgatt gccgacgagg atatggagcg cgagacagaa gagaagacct
     6721 cgtccgtgca tttcctgcgt tttgaacttt ccccggcgat gatcgacgcg ctgcacaatg
     6781 gcgcatcgct gagtatgggt attgcccact cgagctacga tgcgtcatgt gatcccgttc
     6841 agtcgggtgt gcgagattcg ctgataaatg acctggttgc tactgacggc gcttcatgat
     6901 gaagagatta ttcctgttgc tatgctggat accggtgtta gtgccgatag gcgcgtacgc
     6961 cgagaaaacc atggattacg agtttgataa tgaaaagcca tgggccgaga tacaggcaca
     7021 gttgccgccg tatccgaaaa ctgaaaatct gctgcaattt gatgccgggc cagcaagcac
     7081 caattcgcac tatgtcgaca tatcctccct ttccctggga gaagatggcg ttgtccgctt
     7141 cagcctcgtg atcaagtctg cttcgggagc aatgaatgta agctatgagg ggatacgctg
     7201 ccagaccgcc gagaaaaaaa cgtatgcgta cgggcgcaat aataatacct ggctggaggc
     7261 aagggcgtcg agatggacgg atctcgaaaa tatcgcccag aactatgcac aacggacgct
     7321 ttaccgttat tttttctgcc cccttggcat cgacatggta agggatgcgg atgaggcgat
     7381 tcaggcccta aaagcaggcg tgaatccacg tgcagtgcgg tattaggaac tggaattggt
     7441 actcaagtcc gcaacttatc cccaaaccct atcgcgatcg ctcgtatagc gtttttgcgc
     7501 gcattcccat gcggcaatag cctagaactg gcccgggtaa tttatcgacc aaggcccgga
     7561 attggtcaac atgattatcg ttgatctggc ctggaatgac aggcagatag gcaaactcaa
     7621 ggcccagttt ccgtgcttca gattccatta attcatgccc cggatgagcg ccgccatctt
     7681 cactgtcagg ccggttacag atcacgctct tgaaccctgc cgctttgatt tcgttgaggt
     7741 tctccgcgct gatttgtgga gtgattgaaa aattgccgtc gatttcagta attttcatat
     7801 cgatatctcc aaggaagatg caaggaactc gactttttca gatacattct accgcaatcc
     7861 ggttaggaga tttcgtcaga gtagaaccat gttgtcccta tggatgagtt cgggttcgcc
     7921 cagataaccc aggaccgctt cgatttcgtt acttgtccgg cgcaatatcc tgcgggtttc
     7981 cgcggcgcta taattgacga gtccgcgtgc aatttcgttt ccatgggggt cgagacagcc
     8041 gaccacctcc ccgcgttcga aattcccact aacgttcgta acgccgatcg aaaggaggct
     8101 tttcccgcct gacgtgagcg cttccaccgc gccttcgtcc aaggtgatgt tgcctcgcat
     8161 ttgcagttga tcggcaagcc attgcttacg tgcggccagg gtcatggttt ccgccagcag
     8221 ttgcgtaccg atggccgtcc cgtgcgcaag ctgcatcagg acgtccggct catgtccgga
     8281 ggcgatgaca gtatgtgcgc cgctgcgggc cgcccgtttc gcggcaagca cttttgtgag
     8341 catgccgccc cgtccgatgc tgctgcctgc gccaccggcc atcgcttcaa tcgcagcatc
     8401 gcccgccctg gcttcccgca caagcgtcgc tttagtatct ttgcggggat cggctgtaaa
     8461 cagcccacct tgatcagtga gaatcaccag cacatcggct tcgatcagat tggtaaccag
     8521 cgcggccagc gtgtcattgt ccccaaaccg gatttcgtcg gttgccacgg tatcgttttc
     8581 gttgatgacg ggaatcacat tcatgctcag caatgtggtc agggtcgaac gggcattgag
     8641 gtagcgtttc cggtccgaca aatcctcatg ggtcagcaac acctgcgcgg cgtgaaggtc
     8701 gtgggcgcga aagcatgatt catacgcctg caccagaccc atttgcccga ccgcggccgc
     8761 ggcctgcagc tcgtgaaccg cttgtgggcg ccttttccag cccaagcgtt gcatgccctc
     8821 tgcaatagcg ccggaagata ccagcaccac ctccttgccc acttccttca gccgcgcgat
     8881 ttgctcggcc cagcacgcaa gtgcaccgtg atccaaacct tgtccctggt tggtgaccag
     8941 gctgctgccg accttgatga cgatgcgccg tgattgcttt acgatggact gcatgaggtc
     9001 aatcttcgct tgcttttgac gcttcctgct cgagatattc catgatcgcg taggtcaatt
     9061 ccttgcagcc ttcgccagtt atggccgaaa tggcaaaact tttccccttc catcccaggt
     9121 tctggatgaa gttcctgcag gtctttccgc gctcctgttc gggcagaaga tcgaccttgt
     9181 taagcaccag ccaacgcggc ttttcataga gtgcctcgtc atacttcctg agttcttcaa
     9241 cgatcgcttt cgcttcacgc accgggtcta ctgcctgatc atatggtgca acatccacga
     9301 catggagaag caggcgggtt cgggaaaggt gtttcagaaa gcggtagcct aggcctgcgc
     9361 cttccgcagc gccttctatc aatccaggta tatcggccac gacgaagctg cgattctcat
     9421 caacgcgcac caccccaaga ttggggtgca atgtggtaaa cggataatcc gccactttcg
     9481 ggcgagcggc cgatattgcc cgtataagcg tggatttacc tgcatttggc atccccagga
     9541 gccccacatc ggctaaaacc ttcagctcca gtttaagctc gagctcttct cccggttcgc
     9601 ccggagtaaa ctggcgaggc gtacgattgg tactggattt gaagtggagg ttgcccagtc
     9661 cgcctgtgcc ccctttggcg agcagaacct tttcgccatc gcgctccaga tccgcgacca
     9721 cctctccggt agcgatattg gtgatgactg tgccgacagg catgcgaaga gtaatatctt
     9781 cagcactttt tccgtaacaa tcggaaccgc gtccgttttc cccgttcttt gcgcgaaaaa
     9841 tacgtgcgaa gcggtagtcg acgagcgtgt tgatgttgcg gtcggcaacg gcaaaaatgc
     9901 tgccgccccg tccgccgtct cctcccgaag gaccgcccct tggaatgtat ttttcacgac
     9961 ggaaggcggc cgctccatct ccacctttac cagcgatgac ctctatcatt gcctcgtcta
    10021 tatatttcac ggcatgtgtt ctttagtatg tacaaacaaa aaagccctat cataatcgat
    10081 agggcttttt tacatcaaaa tttgaagggt caagccggaa taatgcttac tgttttccgt
    10141 ttttcagaac ctttgaatcc gaagttcacc gtaccggtgg ccttcgcaaa aagcgtatga
    10201 tctttgccca ttcccacgtt atttcctgca tgaaagcggg tgccgcgttg ccgcacgatg
    10261 atgctccctg caggaatcaa ctcaccgcca aaacttttca cgcccaaacg cttggaattt
    10321 gaatcgcgtc cattcctgga acttccgcca gcttttttat gcgccataca atcctcctag
    10381 gccgagatac cggtgatctg gatctcggtg taattctgcc gatgcccctg atgtttctgg
    10441 tagtgtttgc gccgccgcat tttaaaaata cgcaccttat cgtgcctgcc ctgctgaaga
    10501 acagtagcct tgactgcggc gccgctcaat atcggcttgc ccatcgatac attctcgccg
    10561 tctgcaatca tcaatacctg gtcaatcacc agttcactgc cgatatctgc gggtatttgt
    10621 tctatcttaa gccttaggcc gttttcgaca cggtattgct tgcccccggt ctttataatt
    10681 gcgtacatcc tgtcatgctc cattgattgc aatcgctgcc tcgcccgacg attctgaggg
    10741 cgggcaagga aaacaagtat tatacgcagc cggtacattt cagtcaaact actacgccta
    10801 ccgagttcta gaagaagaga accaaaaatt tcaggagcat agcgaacttg acacgttggc
    10861 ggctacgttt ctaccatggc agctttttcc gggaaccttt gtgtcaattg aacgtatcag
    10921 gagcctgatc gccaatgata tggccgccgt ggactcagtc attcgcgaga aactctactc
    10981 cgatgtcgtc cttatccgcc aagtaagtga atacatcatc aatagcgggg gaaaacgtct
    11041 gcgtcccgca ctcgttattc tttccgccgg tgcgttcggt tattccggaa aatatcatca
    11101 cgatcttgca gcgatcgttg aattcattca taccgccacg ttgctgcacg atgacgtcgt
    11161 ggatgcttcg gaactgcgcc gcagcagggc gacggcgaat gcattgttcg gtaatgctgc
    11221 gagcgtactg gtaggagatt ttctctattc cagggcattc cagatgatgg tcgaagtgga
    11281 taacatgcgc gtcatgaggg tactggctga tgcgaccaac acgattgccg aaggcgaagt
    11341 gctgcaattg ctcaacgttc gcgatccgga tgtcgatgaa gagaactatt tgcgcgtgat
    11401 ccgctacaaa accgccaaat tgttcgaggc tgcaacccgg ctcggagcaa tacttggtaa
    11461 cgcgagcgcg gccgaggaag aagcgatggc ggtgtacggc atgcatcttg gcacggcatt
    11521 tcagttgacc gatgatatgc tggattattc cggcgattat caggaaaccg gtaaaaacct
    11581 gggggacgat ctcgctgaag gtaagccgac gctgccactc atcttcgcaa tgagaaccgg
    11641 atcaagtgca caggctgcca tcatccgcaa tgccatagcg cagggaggcg aagggggatt
    11701 tcaacctgtc ctggaggtta tccagcagac tggcgcgtta gcctacgcgc gccggcgggc
    11761 tcaagccgaa tcggacacag cttccaaaac catttcatta ttgcctgatt cggattacaa
    11821 ggaatgcctg ctgcaactgt gcacgtttgc ggttgcccgc aattattaga aagtctctga
    11881 ggatacttcc ttaaaacaaa gtccttacat ttaggatttg ctcgaatacc gcttcgtgcg
    11941 tacaattgct gcccacccgc ttgctggctc gggttcaatg aagcgggtta ttaagccggt
    12001 ttgaaaacgg gatcaattcg gggtgtagct cagcctggta gagtactgcg ttcgggacgc
    12061 aggagtcgga ggttcgaatc ctctcacccc gaccattttt ttccaacgtc aatctttttg
    12121 aaaactcggc atatcggtgc tttttgaacc cccgctctta taggcgtttc acaaaaaact
    12181 gaaaatgctg gctgagtaaa gtatatttcc cggtttggtt tcttgaaccc tctccccgcc
    12241 ccctctccca accggggagg ggttaattct ccctcttttc ctgttgttac tacgtccaca
    12301 gcggctggtg gagctggtaa aaagcaaata gccgccaact cacctggttt tccctgaggc
    12361 tctcaccggc atccaggaat aaaacgggca tgaagaaaac aatctgcggg caaagtgttg
    12421 tagtgggata ttgcctgttt tccccgctcc cacgggtggg agagggggca gcggagaggg
    12481 tgttacaagt tgcatatgtc cgtgcaatca gtagaatgac tttcatgttt cataatcagg
    12541 aaacgcacct tgcgggggct caccaaagga attcgagcgt tgggtagaat cctgtttctc
    12601 atccttgttg cagtactctt ctttttgctg atacgcagct tcgcccgttc gctcaggaaa
    12661 cgccgggaag aaatcgaaaa ggcgcagcgc tcactcgaag gggaggatat ggttcgctgc
    12721 gttcactgcg gggtacattt gccgagaagt gaaagcgtcg tttcagaagg taagtttttc
    12781 tgtagcgacg agcacaggcg cttgcacctt caaccctagc aattctctcg gcccactcgg
    12841 aacgtggcgg agttcgatga aaccgggatt gatggagagg ggtacctggg gaacacacgc
    12901 ttcatcccgt cacccaattt tgacgaacgc ccggaagaat gcggcgtcaa tctgctggtg
    12961 attcacaaca tcagcgtgcc ccctgacgat tttggcggcg agggggtgat cgagctgttc
    13021 acgaacgtgc ttgatcctga ggctcacccg tattaccagc ggcttagggg tctaaaggtt
    13081 tccgcgcact ttttcatccg ccgtcgcggc gaaatcatcc agttcgtgtc ctgtgaaaag
    13141 cgggcgtggc acgctggtga atcatgctgg cagggcaggg ggcgatgcaa cgattattct
    13201 atcggtatag agctcgaagg gagcgattgc atgccgttta ccaacgcgca atacgctgcg
    13261 ctcgcctcgc tcacccatga gctacaggag agatatccca ttcacgatat tgtaggccat
    13321 tccgatatcg cgcccggtcg caagactgat cctggtccct gctttgactg ggctcgttac
    13381 cgcaccgcga tcagccaccg tcgctgagcc gctcggcctc cgccggtttt tattgcctat
    13441 attccaggcg acccgcttgg atttaccggg ccgatgcgcg acgcttttcc ttttccactg
    13501 aagccgtatt gccgcgggca ggaacgggaa aggcgccgat accctggccc tggagataat
    13561 cctcgtagct tcccttgaaa tcggtgattc cttccggctc catttcgata atgcgggtcg
    13621 ccagcgacga cacgaattcc cggtcgtgcg aaacgaaaat gagcgtaccc gtatatttct
    13681 ccagtgcgct gttgagcgac tcaatcgact ccatgtcgag gtgattggtc ggttcgtcca
    13741 tcagcaacac attattgcgt tgcagcatga gcttcccgaa aagcatgcgt ccttcttctc
    13801 ccccggagat gacttgcacc gatttcttta cctcgtcgcc ggaaaacagc aatctgccca
    13861 ggatgctgcg cacggcctga tcgtcgtcat tttcccgacg ccactgcgtc atccagtcgg
    13921 taagcgtcac gtcattttca aagtccgtag agtggtcctg tgcgaaatag ccggaccgcg
    13981 ctttttcggc ccatttcact tcgcccgagt cagggacgag atcgccggcg atacagcgta
    14041 gcagcgtcgt cttgccgatg ccgttagggc caatgatggc gattttctcg cccgcttcga
    14101 cattcatgct gaatttgtgg aatagcggct tttccatgcc gggaaattgc ttgctgatgc
    14161 tttgtatgga aacagccagc ctgtgcagtt tttcgcgttc gtccacttcg atgcggatat
    14221 aaggatattg acggctcgaa ggtttgacat cctccagttt gattttttct atttgccgag
    14281 cgcggctcgt ggcttggcgc gccttggaag catttgcaga gaagcggctg acgaatgcct
    14341 gcaattcggc gatctgggtt ttctttttgg cattatccgc gagcatgcgc tctctcacct
    14401 gggtggaagc gatcatgtat tcgtcatagt tgccggggta gatgcgaatc tcgccgtagt
    14461 ccaggtcagc catatgagtg catacgctgt tcaggaagtg gcgatcatga gagatgatga
    14521 taattgtgct tctgctctga ttgatgacgt cttccagcca gcggatggtg ttgatgtcaa
    14581 ggttattggt gggttcgtcg agcagcagga tgtcagggtc ggcaaacagc gcctgcgcca
    14641 gcagaaccct caacttgaac ccaggcgcaa ttgcactcat catggattga tgctgggaca
    14701 gaggaattcc cacgccaagc agcaattctc cagcccgcgc ttccgcagaa taaccatcca
    14761 gctcggcgaa gcgcgcctcc agctccgcgg catgcatata gtccgcctcg gttgcgtcag
    14821 gattcgcata aattgcgtcg cgctcctgtt tcacctgcca tagctcggca tggcccatga
    14881 tcaccgtatc catgacgaca caatcttcga aggcgaactg atcctgacgc agcttgccga
    14941 cacgctcgcc ggcatcgact gctacattgc ccgaactggg ctccagatcg ccgcccagta
    15001 ttttcatgaa ggtcgactta cc
//
LOCUS       DASSHJ010000210         6355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1447946,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000210 DASSHJ010000000
VERSION     DASSHJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447946"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..796
                     /locus_tag="VFI43_08260"
     CDS             380..796
                     /locus_tag="VFI43_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET7062153.1"
                     /translation="MAGLSKDTPRPSEIKLHRKSRVLEISFSDGRIFQFPCEFLRVYS
                     PSAEVRGHNPGQEVLQTGKKEVGISHIEPVGNYAIQLNFSDGHNTGLYSWDLLYDYGL
                     HQEEMWRRYLHRMEEAGASREPVTSSRGFSPHEGKK"
     gene            862..1596
                     /gene="ubiE"
                     /locus_tag="VFI43_08265"
     CDS             862..1596
                     /gene="ubiE"
                     /locus_tag="VFI43_08265"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET7062154.1"
                     /translation="MSKTTHFGFNTVAEAEKAHKVAGVFDSVAERYNLMNDLMSAGMH
                     RLWKHFAVQASGVQAGARVLDIAGGTADLSALFLEQVGENGEVWLTDINNSMLSIGRD
                     RLLDSGTAVPVAQCDAEKLPFPGNYFDCVSVAFGLRNMTHKESALKEMLRVLRPGGRV
                     IVLEFSRVWKPLQRLYDAYSFNLIPALGGVIAQDRDSYRYLVESIRMHPSQEELKQLM
                     QQAGFERVQYFNLAGGVVALHRGYKF"
     gene            1658..2098
                     /locus_tag="VFI43_08270"
     CDS             1658..2098
                     /locus_tag="VFI43_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7062155.1"
                     /translation="MYRRILVPLDGSSTSERALREAIGLAREQGAQLELLHVMEDILF
                     LENEAYINYSEVRKSVRGEGERMLSQALSAAEQAGVTAEQKLLDADGARTASVIVDEA
                     RRWAADLIVIGTHGRSGFSRILFGSVAEGVVRTAQIPVLLIRGH"
     gene            2509..3420
                     /locus_tag="VFI43_08275"
     CDS             2509..3420
                     /locus_tag="VFI43_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7062156.1"
                     /translation="MADLRPRVLLFGELLVDRFPEGDVFGGAPFNVARHLSAFGCAPT
                     LVTRIGMDQEGGHALQVLESAGLPTHGVQLDSIYPTGTVAVRFTSSGVHRFDILPNQA
                     YDHIHPRLARIAAIATRPQLVYFGTLAQRSDSHRALRHLLRAATGQRFLDVNLRSPWL
                     DVPRLHWSLKQADIVKANDAELDLMGDLLDLGESTAEAKAELLMRRYALKGVLITLGP
                     EGAWWLDDGGWIRIASVPVEELRDTVGAGDAFSSVFILGLLHRWEVPLTLQRAHRFAG
                     AVCGILGAVPEHPRFYSPFISEWFPAG"
     gene            complement(3463..4215)
                     /gene="epsA"
                     /locus_tag="VFI43_08280"
     CDS             complement(3463..4215)
                     /gene="epsA"
                     /locus_tag="VFI43_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HET7062157.1"
                     /translation="MDFDFPLNPEHAQRYARIIEEGACVRRHYDLLIWLQGEIQSYLP
                     HEIMLAACGDFNSGFIRYDLISALLGIRTDCSNIKALAPLLQGAFNRWVHLGKAPYIL
                     GRGDSVFLLEEYGLQCALGVTLRGMRSALIHGVSDKRGQEDSLYIAFSSNEKLDISAI
                     NAMRFLLPYLDMAMGQMELAARNPQPAALRPNVKVDVLTEREEGLISLIKAGKTNVEI
                     EALLQISPIALKSQLRNMFRKLYEPDAKNPKG"
     gene            4981..5415
                     /locus_tag="VFI43_08285"
     CDS             4981..5415
                     /locus_tag="VFI43_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062158.1"
                     /translation="MNKKEVIFALTVFLSFPLAAMAVQQVQTEQSQAQPPLHGDGWRL
                     VRSIQLGTSKHFIHMVLIDSKRDMDKAVYGAALSKICRSEPDFCRVRFWNEERLVPSA
                     ISFTDAQFKGMRAEYVFNRAGNVQEMRYACTVVSDKNQCFSH"
     gene            5487..5876
                     /locus_tag="VFI43_08290"
     CDS             5487..5876
                     /locus_tag="VFI43_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7062159.1"
                     /translation="MPRQLISSGSTFEQEIGYSRAVVEGDWIFVSGTTGFDYSKMTIS
                     DDLLEQAEQCFRNIQSALNEAGAFLQDVVRVTYVLPEASDFPKCWPVFRKYLGQVRPA
                     AMMIAAGLSDPRMRIEIQVTARRGSSS"
     gene            5955..6344
                     /locus_tag="VFI43_08295"
     CDS             5955..6344
                     /locus_tag="VFI43_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062160.1"
                     /translation="MAAKTNKVFISFALRDIKLRDQLLEQLNKEQTTYSFVDMPVKQS
                     WEPAWKEECREKVTGCDGVIAIITKNVVRADGQLWEMHCAYEGRMPVLLIHGDAEKLP
                     SKLPEPVGSREIDAWTWPTISTFLNRL"
BASE COUNT         1446 a         1574 c         1766 g         1569 t
ORIGIN      
        1 ggccggtcag ttgctggtca tggccgatgt tccgaaagac cgggttgaag aaatcgaagg
       61 catcataaaa tctcatctgc cccaggtgga tatagagggg accgaaccaa gaataccggc
      121 atttccctga tcctcgggca gtacgcggga aatcatgacc tgccgctggg caggtcatat
      181 tgtgtctgct ccctcgctcg ttattgttgg aaagtggatc ggcttgattg cccatgctgt
      241 cccttgaaac tggcctcctt tgaaacgatc gagcatccag caagcaattg caatcacgtc
      301 tcaaaatttc cccagggttt cggtttagct tgcactggtt tatcattcgc gctcccgaat
      361 tgcgaaaacg acgaaaacga tggcaggttt gagcaaagat acgccacgcc ctagcgaaat
      421 aaaactgcat cgaaagtctc gcgtgctgga aatttccttc agtgacggtc ggatctttca
      481 atttccgtgc gaattcctga gggtctattc gccgtccgcg gaggtgcggg ggcacaatcc
      541 tggacaggaa gtgctgcaaa ccggaaagaa agaggtcggg atcagccata ttgagccggt
      601 cggaaattat gcaattcaat tgaatttttc cgatggccat aatacgggtc tctattcctg
      661 ggatctgctc tatgactatg gcttgcacca ggaagaaatg tggcgccggt acttgcaccg
      721 aatggaagaa gccggagcaa gccgggagcc ggtcacaagc tcgcgcggct tctccccgca
      781 cgaggggaag aagtagtttt ctggtctgcc cttcttatcc agcccgacaa tccgaccgaa
      841 cgtctcaata acttattatt catgtcgaaa accacccatt tcggtttcaa tacggttgct
      901 gaagcagaga aggcccataa agtggccggc gtattcgatt cggttgctga acgttacaac
      961 ctgatgaacg acctgatgtc cgcgggtatg caccgtttgt ggaagcattt cgcggtgcag
     1021 gcgagcggag ttcaggcggg tgcgcgggta cttgacattg ctggcggcac cgctgacctg
     1081 agcgccctgt tcctggagca ggttggagag aatggagagg tctggcttac cgatatcaat
     1141 aattcaatgc tctcgattgg tcgtgaccgg ctactggatt ctggcactgc cgtacccgtc
     1201 gcacagtgcg acgcggagaa attgcccttc ccgggcaatt attttgattg cgtcagcgtc
     1261 gcgtttggcc tgagaaacat gacgcataag gagtcagcat taaaagaaat gctgcgcgtt
     1321 cttcgccccg gcgggcgcgt gattgtgctg gaattttcaa gagtgtggaa gccgttgcaa
     1381 cgcttgtacg atgcctattc ctttaatttg atccccgcct tggggggcgt catcgcccag
     1441 gatcgggaca gctatcgcta tcttgtcgaa tcgatacgga tgcatccttc gcaggaggag
     1501 ctaaagcaac tgatgcaaca ggccgggttt gagcgggtgc aatatttcaa cctggccggc
     1561 ggtgtggtcg cgctgcaccg aggttacaag ttctaggagc atatcgacta cgcccggctt
     1621 gcccggtcgg atcaatggat cggctatagg agaaaatgtg tacaggcgca tcctggttcc
     1681 tctggacgga agctctactt ccgaacgcgc actgcgagaa gccattgggc tcgcgaggga
     1741 gcagggggcg caactcgagt tgctgcacgt gatggaagat atcctgtttt tagaaaacga
     1801 agcgtatatc aactattccg aagtaaggaa gagtgtacgg ggggaggggg aacgaatgct
     1861 ttctcaagcg ttgtcggcgg cagagcaggc aggtgtgaca gcagaacaaa aactgcttga
     1921 tgcggatggc gcgcgcactg ccagcgtgat tgtcgatgaa gcgcgccgct gggcggctga
     1981 ccttatcgtt atcggcacgc atgggcgttc aggttttagc cgcatcttgt tcgggagcgt
     2041 tgctgaaggt gtggtacgaa cggctcaaat accggtatta ttaattcgcg ggcactagat
     2101 gtttctctcc ggtggccgga cccggaaaaa agagcatatg cagactcgag agtaatgaac
     2161 tgaacagatt gctcaattcc ctggcattct cctttttttc ctggagcctg agaataggca
     2221 cttggatgcg agccgcatca actgatcatt atgagcttgt tataccgaca agctatcgta
     2281 agcgaacatc aacgaacccc ggcattgagc tctttgtccg caagtttcct gaaaggcgcc
     2341 gttgattgtg aatgtagtcc gccgcgattg gcgttcgact ttgactgctg agggctctgc
     2401 tgtatcccca atcgggacaa gtcgagcacc gcgattcttc cggtaaatat ccttcgtcat
     2461 ttcttttttt tcttgctcct cctacactca agtatttgag agtaagaaat ggccgacctt
     2521 cgcccacggg tactcttgtt cggcgagctt ctcgtcgaca ggtttccgga gggtgatgtc
     2581 ttcggcggcg cgccatttaa tgtggcaagg catttaagcg ccttcggctg cgcgcctacg
     2641 cttgtgacgc gcattggcat ggatcaagag gggggtcacg ccctgcaagt cctcgaaagc
     2701 gcagggctgc cgacgcatgg tgtgcagctc gattccatct accctaccgg gacggttgcg
     2761 gtaaggttta cttccagtgg cgttcatcgc ttcgatattc ttcccaatca agcatatgac
     2821 catattcatc cgcgcctggc ccgcatcgcc gccatcgcga cccggccgca actggtatac
     2881 ttcggaacat tggcgcagcg ctcggattcc catcgcgcat tgcgccatct cctgcgcgca
     2941 gcaacaggcc agcgattcct ggacgtcaat ttgcgctctc cctggctaga tgttccgcgc
     3001 ctgcactggt cgctgaaaca ggccgatatc gtcaaagcga acgacgcaga gcttgatctc
     3061 atgggcgatt tgctcgattt gggtgaatcg acggccgaag ccaaggcgga actgctcatg
     3121 cgccgctatg cgttgaaggg ggtgctgata acattgggcc cggagggagc gtggtggctc
     3181 gacgatggag ggtggatcag aatcgcttct gtacctgtcg aggaattacg ggacacggtg
     3241 ggtgcgggtg acgcgttctc ctcggttttc atactcgggc ttttgcaccg atgggaagtt
     3301 ccccttaccc tgcagcgcgc acatcgtttc gcgggggccg tgtgcggcat actgggagcg
     3361 gtccccgaac accctcgttt ctacagtccc ttcatcagcg agtggtttcc tgcaggctga
     3421 aaagggtttg atttttttga tgtaaggctc gtggcggtac tctcaacctt tcggattctt
     3481 ggcgtccggt tcataaagct tcctgaacat attccgcaat tgactcttga gggcaatcgg
     3541 actgatctgc aataacgctt cgatttccac attggtcttg ccagccttga tcaagcttat
     3601 tagtccttct tcccgctccg ttaacacgtc aactttcacg ttcgggcgta atgctgcagg
     3661 ttgcggattg cgcgcggcaa gctccatctg gcccatggcc atatcaagat aagggagcag
     3721 aaatctcatc gcatttatgg cggatatatc aagcttctca tttgagctga acgcgatata
     3781 aagactatcc tcttgtccgc gcttatcgct gaccccatgt atcagtgccg aacgcatgcc
     3841 gcgcagcgtc actcccaagg cgcattgcaa accatattcc tcgagaagga ataccgagtc
     3901 cccgcgtccc aggatgtagg gggctttgcc caagtggacc cagcgattga atgctccctg
     3961 gagcagtggc gcaagcgctt taatgtttga gcaatctgtc ctgatcccga gcagggcgga
     4021 gataaggtca tagcgaatga aacctgagtt gaaatctccg caggctgcaa gcatgatttc
     4081 gtgtggcagg taggactgga tctcgccttg aagccatatg agaaggtcat agtgccgccg
     4141 cacgcacgcg ccttcctcta ttatgcgagc gtaccgctgg gcatgttcgg gatttagtgg
     4201 aaagtcaaaa tccatatcta ttatttcttc ttgaggagta cgagtcctgc ttgtaggaag
     4261 gtttattcag gacggtctcg caaattcttt cgtaattcat tatcaactac tccttcttag
     4321 caaaaaccat gccttgatcc tacttcattg ataattgaca taaacacaac tatctcgaga
     4381 tgctggtgta agcatttccg gcgttaatcc ggacgggccc tttgtccttg tataagtaaa
     4441 tgcggttcga tctctggccc gaatggaatt tcattttttg ccggccaggc aactgatcgc
     4501 tttttccaat acttgagcgg acgagaagcg catgtcagcc attgctggac aaaaaacgac
     4561 aaatacaatc aactgcttgc cgccttcacc atcccgcaaa tttggcacta tctgttaggc
     4621 gatgagtggc attgaaccag ggacctagaa cgggggacgg ctgaagggaa accggtcaaa
     4681 tgcgcactct ttcatgagtt tctgaattct ggattggtac taccggtttt aagccggggg
     4741 atcgatttcc ttgttggcaa caatcgttat ttgatagaat cgattcgatg aacaactctt
     4801 gcagaacgca tgaaagatga attccgtgtc ttttccgggg gtcttgacat tcaagttgga
     4861 gggacagcct gaggtatccg ctcgcatttg tgccgcccgt tctagtcagg gaatgcgcgg
     4921 gcgtgcactg tttaaacact cattgaatcg gattatggtt ggagatcagc aggaccatct
     4981 atgaacaaaa aagaagttat cttcgcactc accgtctttc tctcgtttcc gttggcagca
     5041 atggcagtac aacaggtcca aaccgagcaa tcccaggctc agcctccgct ccatggggac
     5101 ggatggcggc ttgtacggtc tattcagctc ggtacgtcaa aacacttcat tcacatggtg
     5161 ctgatcgata gcaagcgtga tatggacaag gcagtctatg gggccgccct cagcaagatc
     5221 tgccggtccg agccggactt ttgcagagta cggttctgga atgaggagcg gcttgttcct
     5281 agtgcgattt cgtttacaga cgcgcaattc aaggggatga gagccgagta cgttttcaac
     5341 cgcgcaggaa atgtgcagga aatgcgttat gcctgcacgg tcgtgtctga caagaatcag
     5401 tgtttttccc attgattctc cctcttgtca tgcaccgaag ttcctgaaag gacgggaaag
     5461 cttatgaaaa gagataaggg attcagatgc cacgccagtt gatcagctcg ggctctacct
     5521 tcgagcagga gatcggctat tcaagggccg tcgtggaggg ggactggata tttgtttccg
     5581 ggacgacagg ttttgactac agcaagatga cgatttccga cgatctgctg gagcaggccg
     5641 agcaatgttt caggaatatt cagagcgcgt tgaatgaagc cggcgccttc ctccaggatg
     5701 ttgtcagggt gacttatgta ttgccggaag catcggattt tcccaaatgc tggcctgtct
     5761 ttcgcaaata cctgggccag gttagaccag ctgcgatgat gattgctgcc ggattgtccg
     5821 atccccggat gcgcatcgag attcaggtaa ctgctcgccg cggttcaagt agctgaatca
     5881 ggcaatatcc tgcctggaga agtagggatc ttttgcttgt gtcgaattac aataatcgat
     5941 tgagggaggt atgaatggct gcaaaaacga acaaggtttt catcagcttt gcgctgaggg
     6001 atataaagct gcgtgatcaa ctgctcgagc aattgaacaa ggagcaaaca acatactcgt
     6061 ttgtggatat gccggtcaag cagtcatggg aacctgcctg gaaagaagag tgtcgcgaga
     6121 aggttaccgg ttgtgatggc gtgattgcca tcattacaaa aaacgttgtg agggcagatg
     6181 ggcagttgtg ggagatgcac tgtgcctatg aaggaaggat gcccgtgctg ttgatccatg
     6241 gcgatgccga aaaattgccc tcgaaactgc ctgaaccagt gggaagccgt gagattgacg
     6301 cttggacctg gcctaccatt tcgaccttcc tgaacagact atagaagttc agcca
//
LOCUS       DASSHJ010000211         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_949626, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000211 DASSHJ010000000
VERSION     DASSHJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949626"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFI43_08300"
     CDS             complement(<1..325)
                     /locus_tag="VFI43_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET7062161.1"
                     /translation="MPANYIDPGLIAVLEKHAQPLRGSSNDYDNLIQAARGKKFVLLG
                     EASHGTAEFYRVRAEITRRLIEEEGFDAVAVEADWPDAYRVNRFVSRMSDDTDADMAL
                     SDFERF"
     gene            complement(412..1083)
                     /locus_tag="VFI43_08305"
     CDS             complement(412..1083)
                     /locus_tag="VFI43_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7062162.1"
                     /translation="MPRPQIAETKEVRIPTSGIWLNADLAIPPEPKGIIAFAHGSGSG
                     RHSSRNRYVATTLNQYRFATLLADLLTEEEEIVDMRTRHLRFDIPMLADRLITIAAWL
                     QEEHQTKQLKIGWFGASTGAGAALIAAARRPENIFAVVSRGGRPDLAGDYLRDVTAPV
                     LLIVGGNDPQVLELNEIALTRLNAQSRLEIVPHATHLFEEPGTLEIVARLAGEWFQQN
                     IPEKR"
     gene            complement(1373..2734)
                     /locus_tag="VFI43_08310"
     CDS             complement(1373..2734)
                     /locus_tag="VFI43_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7062163.1"
                     /translation="MSDSHPKILIVDDDTDLLELLSIRLTAAGYKVDAVESAEAALNY
                     LDVTRPQLVISDMQMSGMDGMALFEYIHRSLPTLPVIILTAHGTIPDAVAAVQRGVFG
                     YLAKPFDSKTLLANIAQALRFVQGAVAQNEVMQASWRRNIITQSAVMEDILTKAGLVA
                     EGDASVLIYGESGVGKELFAHAIHEASKRRANPFVAINCAAIPEQLLESELFGHVKGA
                     FTGAVRDHKGLFHLAESGTLFLDEIGDMPVLLQVKLLRVLQERKVRPVGSAHAVPVDV
                     RIISATHRDLKAEIAAGNFREDLYYRLDVVALTIPVLSQRREDIPLLTNYFLNTFSEK
                     YQKNINGFSPEAMEMLVSASWPGNVRQLMNVVERCIVLSTAPLISAVLVYDAMHREEE
                     QLVSFEEARKSFERDYLVRVLKISSGNVTQAARLAKRNRTEFYKLLQRYQLDPSVFKQ
                     PHG"
     gene            complement(2749..3279)
                     /locus_tag="VFI43_08315"
     CDS             complement(2749..3279)
                     /locus_tag="VFI43_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acyltransferase"
                     /protein_id="HET7062164.1"
                     /translation="MTRYYHARVIGLAIALVSLIGCSAIPEQQPCQCPPAVEQVVTGQ
                     MDDMMQYYESLRKLPTPDLAKLHERVKQNFAQNKSDANRLRLVLLLTLPQTSFRDIQG
                     AINLLNEWPRDAKPNNLQSFRNLLASFLADQQRLSTHVEEASQKLKEEQKRADMLQHQ
                     IDALKSMEKNLILIQP"
     gene            complement(3276..>3626)
                     /locus_tag="VFI43_08320"
     CDS             complement(3276..>3626)
                     /locus_tag="VFI43_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7062165.1"
                     /translation="GQKIKTIVDNLLSNAVKFSPQRGRISIWANRIEESIVLNVLDAG
                     AGIDEADRDKVFEPFYQGRQMHNSPIRGTGLGLSIAREYALAHGGNIELIPQPAAGAH
                     FRLTIPIRDPEGSS"
BASE COUNT          787 a         1002 c          984 g          853 t
ORIGIN      
        1 gaaatctttc gaaatctgac aaagccatgt cagcatccgt gtcatcggac atgcgcgata
       61 cgaagcgatt gacgcggtag gcatcgggcc agtccgcctc cacggctacc gcatcaaagc
      121 cctcttcctc aatcaaccgg cgagtgattt cagcacgcac ccggtaaaat tcagctgtgc
      181 cgtggctcgc ttcgccaagc aggacaaatt tcttgccgcg agccgcttgt atcaagttgt
      241 cgtaatcatt ggaggagccg cgcaagggct gcgcatgctt ctccagcacg gcaatcaatc
      301 cgggatcgat atagttggca ggcataagaa ttaatcctcc attccttttg cacgtcagcg
      361 aaaaaacgtt cgctagacat aaacccttca ctatttcagc tcgtcaaaac gctacctttt
      421 ctccggtatg ttctgctgaa accactcccc agccaggcgt gcaacgatct cgagcgtgcc
      481 tggctcttca aacaggtgtg tcgcatgagg gacgatttcc agtcttgact gcgcattcag
      541 ccgcgtcagc gcgatttcgt taagctcgag aacttgggga tcattccccc ctacgatgag
      601 aagtacaggg gctgtcacgt cccgcaggta atcgcccgcc aagtcgggac gtccgccgcg
      661 ggaaactacg gcgaaaatgt tttctggacg gcgtgctgcc gcgatcaacg cagcgcccgc
      721 acctgtgctc gccccaaacc acccgatttt taattgcttt gtctgatgct cttcctgtaa
      781 ccatgccgct atcgtgatga gccggtcggc cagcataggg atgtcgaaac gcaggtgccg
      841 cgtccgcatg tcgacgattt cctcttcttc cgtcaggaga tcggcgagca gggttgcgaa
      901 ccggtattga ttgagtgttg tggcgacgta gcgattccgc gaactgtgcc ggccgctccc
      961 gctcccgtgg gcaaaggcga taataccctt tggctccggc ggaatggcga ggtcagcatt
     1021 aagccaaatc ccgctggtgg gaatccggac ttcttttgtt tccgcgattt gaggccgagg
     1081 cattgcggaa aaatctctaa agaggattgg aagctctttt ttatacgaaa aacaactcca
     1141 tttccgtacg atttgccaca taggagcgga aatgctactg gccctgtggc atccgccgat
     1201 tttttgctgg aggactacgt cacagactga cgtaggtgaa gatgccgcgt cgcccgtgag
     1261 attcggcgat tgcacaagtt gcgaaggaca gcgaccgaca caagttcatt gaaggctaac
     1321 tcgatgcacc cttgcctcct cgccagtgag ggcatgatgc catgggatgg agttagccat
     1381 gcggttgctt gaaaacggaa ggatcgagtt gatacctttg gaggagcttg tagaattccg
     1441 tgcgattgcg cttggccagg cgcgcggcct gagtcacgtt tccgctcgat attttgagga
     1501 cgcgaacaag atagtccctt tcaaaactct tgcgggcttc ttcaaacgaa acgagctgct
     1561 cctcttcccg gtgcattgca tcatatacaa ggaccgccga aatcagcgga gccgtactga
     1621 gcacgatgca tctctccaca acgttcatca gttgcctcac gttacctggc catgaagcgc
     1681 tcacaagcat ttccatcgct tcaggggaaa atccgttgat gtttttctga tatttctcgg
     1741 agaatgtgtt gaggaagtaa tttgtcagca agggaatatc ctcccttctc tgggacagga
     1801 cgggaatggt gagcgccacg acatccagcc tgtaataaag gtcctcccta aaattgcccg
     1861 cagcaatctc ggccttgaga tcgcggtgag tcgccgaaat gatgcgaaca tcgacgggta
     1921 ccgcgtgcgc agaacccacg gggcgcactt tccgctcctg gaggacccgc aacagcttga
     1981 cctgtagaag cactggcatg tcaccgattt catcgagaaa tagcgttccg ctttctgcca
     2041 gatggaacaa gcccttatgg tcccggaccg ccccggtaaa tgcccctttg acatgcccga
     2101 atagctcgga ttcaagcaat tgctccggaa tcgccgcgca atttattgca acaaacgggt
     2161 tggcgcggcg cttggaggcc tcgtgtatgg cgtgggcgaa cagttctttg ccgacaccgc
     2221 tctcgccgta gatcagcacg ctggcgtcac cctccgcgac aagccccgcc ttggtcagga
     2281 tatcctccat caccgcgctt tgcgtgatga tattcctgcg ccaggatgcc tgcatcacct
     2341 cgttctgggc cacggccccc tggacaaacc tcagcgcctg cgcaatattg gccagcaatg
     2401 tcttgctgtc gaagggcttc gccaaatagc cgaacacgcc tctttgaacc gccgctacgg
     2461 cgtcaggaat tgtcccgtgc gcagtcagga taataaccgg cagtgtcgga agactgcgat
     2521 gtatgtactc gaacaaggcc attccgtcca tgccgctcat ctgcatgtcg ctgatgacca
     2581 gttgagggcg tgtcacgtcc aggtaattga gtgcggcctc ggcgctttcg acggcgtcga
     2641 ccttgtatcc cgcagctgtc aggcgaatcg acaacagttc gagcaggtcg gtatcgtcgt
     2701 ccacgatgag aatttttgga tgggaatctg acattggcgg tgttggtatt agggctgaat
     2761 gagaataaga tttttttcca tgcttttcag agcgtcgatc tgatgttgca gcatatcggc
     2821 tcgcttctgc tcctccttca gtttctgcga cgcttcctcc acgtgggtgc ttagccgttg
     2881 ttgatctgcc aggaatgatg cgaggaggtt cctgaagctt tgtaaattgt tcggcttcgc
     2941 gtcgcgaggc cattcattga gcagattgat cgccccctga atgtcacgaa aagaagtttg
     3001 cggcaaggtt aaaagaagca ccaggcgaag gcgattggcg tcactcttgt tctgcgcgaa
     3061 attctgcttc acgcgctcat gcaactttgc gaggtcggga gtaggcagct ttcgcaatga
     3121 ctcgtaatat tgcatcatgt catccatctg ccccgtcacg acttgctcga cggcgggtgg
     3181 gcactggcac ggctgttgct cgggaatcgc ggaacaccca atcagggata cgagagcaat
     3241 ggcaaggcca atcacccgcg catggtagta ccgggtcatg aggaaccttc cggatcacga
     3301 atcggtatgg tcaggcggaa atgagcaccg gccgcaggct gtgggatgag ttcgatattg
     3361 ccaccgtgag ccaaggcata ttcacgagcg atggagagcc ctaaaccagt gccgcgaata
     3421 gggctgttat gcatttgccg tccctggtag aagggctcga agaccttgtc acggtccgct
     3481 tcatcgattc cggccccagc gtcaagaacg ttcagtacga tcgactcttc tatcctattc
     3541 gcccagatgc tgatgcggcc gcgttggggc gaaaatttca cagcattcga taacaggttg
     3601 tccacgattg tttttatttt ttgtcc
//
LOCUS       DASSHJ010000212         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_462394, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000212 DASSHJ010000000
VERSION     DASSHJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462394"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="VFI43_08325"
     CDS             <1..93
                     /locus_tag="VFI43_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET7062166.1"
                     /translation="TLADWVLRDRLPVRMQIQLHKLLWGEGPGK"
     gene            118..801
                     /gene="queC"
                     /locus_tag="VFI43_08330"
     CDS             118..801
                     /gene="queC"
                     /locus_tag="VFI43_08330"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET7062167.1"
                     /translation="MAKAVVMLSGGLDSATTLAIAKQEGYECYALSVDYGQRHASELV
                     AAANLAGFLGACQHRIIQIDLAAFGGSVLTDKSQAVPVSGTSSGIPTTYVPARNTIML
                     SLALAWAEVLQSRDIFAGINAVDYSGYPDCRPEYVTAFQKMANLATKAGVEGRRLSIH
                     TPLLALSKADIIRRGIELGVDYSLTVSCYQADEAGYACGLCDSCQLRRSGFEAANVPD
                     STRYRPTPR"
     gene            859..1557
                     /gene="aat"
                     /locus_tag="VFI43_08335"
     CDS             859..1557
                     /gene="aat"
                     /locus_tag="VFI43_08335"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7062168.1"
                     /translation="MIPWLNPADPFPSVTTALVTPNGLLAAGGELSPQRLIEAYRLGI
                     FPWFNEGDPVLWWSPNPRMVLFPNELKISRSLKKTLRKAQYEVRVDTAFLEVMWGCAA
                     PRNGHVGTWIHDEVIAAYNFLHERGFAHSVELWIDGELAGGLYGVSLGRMFFGESMFS
                     RVSDASKIAFVHLVRQLQRWGFGMIDCQMKTAHLASLGAREIPRSEFGQRLKELVNYP
                     GRIGKWSFDNEPVE"
     gene            1541..2275
                     /locus_tag="VFI43_08340"
     CDS             1541..2275
                     /locus_tag="VFI43_08340"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HET7062169.1"
                     /translation="MSQLNDLPSSVLQFYTTASYPCSYLPEQLARSQVATPSHLIDTS
                     VYGELVQAGFRRSGAFTYRPHCDHCRACVPVRVTVDKLSLRRSQRRSWKRHENLIATQ
                     HDLYYHPDHYALYLRYQARRHSGGGMDRDSREQYRHFLLPSNVDSRLVEFRENGQLRM
                     ISIIDELPDGISSVYTFFDPDVPGASYGTYNILWQAHLCLELKLPYLYLGYWIKDSGK
                     MSYKAGFRPLEGLIDGKWEALPDVDS"
     gene            complement(2339..3274)
                     /locus_tag="VFI43_08345"
     CDS             complement(2339..3274)
                     /locus_tag="VFI43_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7062170.1"
                     /translation="MKLISFLLISALASIAVPAAAMSEWDRLNNELESLYQQGEYARA
                     IPVAKRALEAAEKTLDAENPALATSLNSLALLYNAQGQYAKAEPLLKRALAIFEKSMG
                     TESEEVAATLNNLGMLYKNRGDYLQAEPLYKRALEIDEKVLGSEHPDLAVSLNNLAQL
                     YQALEQYSKAEPLLKRALAISEKALGSNHNQVALSLNNLALLYAAQKKYAEAEPLLKR
                     VFSISKKTFGPNHPNVAMSLNNLAGVYRDQGKYPFAEPLFHRALAISEKAFGPDHPNV
                     ATGLENLAQLYRDIGYSSKAVSLDKRAAEIRAAKP"
     gene            3424..4467
                     /locus_tag="VFI43_08350"
     CDS             3424..4467
                     /locus_tag="VFI43_08350"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET7062171.1"
                     /translation="MLYSLLRPLLFRLDPETAHRLTFEAIETAHRFRLLTPASVPCAS
                     RTVMGLDFPNPVGLAAGLDKNGEHIDALAALGFGFVEIGTVTPRPQPGNPKPRLFRIP
                     EANAIINRMGFNNLGVDRLLENVKRAAPPCVLGINIGKNFDTPVDKAQDDYLACLDKV
                     YRFADYVVVNISSPNTPGLRQLQEAGQLDSLLSGLKAAQRKLADTHGKYTPVAVKIAP
                     DLDPVQIESIAALLMTHQIDGVIATNTTISREGVEKLPHSEESGGLSGAPLRDQSTKV
                     VRDLNVRLKGALPIIAVGGIMSPLDAKAKMDAGASLVQIYSGLVFRGPQLVQEIGRAL
                     CNPDQAFQYSASP"
     gene            4464..>5222
                     /locus_tag="VFI43_08355"
     CDS             4464..>5222
                     /locus_tag="VFI43_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HET7062172.1"
                     /translation="MTRDQLAAAIDAILPQTQCRQCGFPGYHPYAAAIAEGQADINQC
                     PPGGEAAIEKLARLLGISPKPLNTAHGIPKPRAVAFIDEQACIGCTLCADACPVDAIV
                     GAARQMHTVITGECTGCELCIAPCPMDCITMVPVQLAATGHPRTALVTERGYPAEEDE
                     KEAADRARARYQFRLKRLERDRREKEEAKEDRQKEKAEEGLLSLEIESQNFELDLDEK
                     PVKESQDRKSAMIRAALKRAMAARAQQLGAEDRTK"
BASE COUNT         1114 a         1404 c         1521 g         1183 t
ORIGIN      
        1 acccttgcgg actgggtatt gcgcgatcgg ctgccggtgc gcatgcagat tcagttgcac
       61 aagcttttat ggggagaggg accggggaaa tgaaaatccc gtgccgtttt ggtccgcatg
      121 gctaaggctg tcgtgatgct ctcaggcggc ctggactctg ccacaacgct ggccattgcc
      181 aaacaagaag gctatgaatg ctacgcgctg agcgttgatt atgggcagcg ccatgcatcc
      241 gagctcgttg cggccgcgaa cttggccggg ttcttgggcg cgtgccagca tcgaatcata
      301 cagatcgatc tggctgcgtt cggcgggtcg gtacttacag acaaatctca ggcggtgccg
      361 gtgtctggaa ccagctcagg cataccgaca acctatgtgc cggcccgtaa caccatcatg
      421 ctgtcgttgg ccttggcttg ggctgaggtg cttcaaagcc gggatatttt tgcaggtatc
      481 aacgcggtgg attattcggg gtatcctgac tgccggcctg agtatgtgac cgctttccag
      541 aaaatggcga atctagccac caaggccgga gtcgaaggcc ggaggctaag cattcacacg
      601 ccgcttctcg cgctctcgaa ggcggatatc attcgtcgcg gaatcgagct gggcgtggat
      661 tacagcctta cggtgtcttg ctatcaggcg gatgaagcag gctatgcctg cgggctctgc
      721 gattcctgcc agctgcgccg gtccgggttc gaggcggcaa acgttcccga ttcgacgcgt
      781 tatcggccga cgccgcggta gctcttcacc cgttatccat ccaggggttg tcctggatcg
      841 tgcgcattcg ctagccttat gatcccctgg ctcaatcctg cagacccctt tccctccgtc
      901 accacggccc ttgtaacgcc caacggactg cttgccgcag ggggcgagtt gtcaccacaa
      961 cgattgatcg aggcctatcg cctgggtatt tttccctggt tcaacgaagg ggatccggtc
     1021 ttatggtgga gtccgaatcc gcgtatggtt ctcttcccga atgaacttaa aatatcccgc
     1081 tcgctcaaaa aaaccctgcg gaaagcgcaa tatgaagttc gggtggatac ggctttcctc
     1141 gaggttatgt ggggatgtgc ggctccccgc aacggacatg tggggacatg gattcacgat
     1201 gaagtgattg cggcgtacaa cttcctgcat gagcggggct tcgcccactc cgtggaactg
     1261 tggatcgatg gagaattggc aggtggactt tacggcgtct cgctgggtag gatgtttttc
     1321 ggtgaatcaa tgttttcgcg cgtttcggac gcatcgaaga ttgcttttgt tcaccttgtg
     1381 cgccagttgc aacggtgggg cttcggtatg atcgactgcc agatgaaaac agctcacctc
     1441 gcatctctgg gcgctcgaga aatccctcgc agcgagtttg gtcaaaggtt gaaagaattg
     1501 gtaaactatc cgggacgaat cggaaaatgg tcttttgata atgagccagt tgaatgatct
     1561 gccctcatcg gtactgcagt tttacaccac tgcatcctac ccttgcagct atttgccgga
     1621 acagttggcg cggtcccagg ttgccacgcc gagccacctc atcgatacaa gcgtgtatgg
     1681 cgaacttgtc caggcaggct ttcgccgtag cggcgccttt acctaccgcc cacactgcga
     1741 tcactgccgc gcatgcgtgc cggtgcgggt gacggtggac aagctctcgc tgcggcgttc
     1801 gcagcgccgt tcgtggaaac ggcatgagaa cctcatcgcc acccagcatg acttgtacta
     1861 ccatcctgat cactacgcgc tttatctgag gtatcaggca cggcgccaca gcggcggcgg
     1921 catggatcgc gacagccgtg agcaataccg gcactttctg ctgccgagca atgtcgactc
     1981 gcggcttgtg gaatttcgcg aaaacggaca gttgcgcatg atcagcatca tcgatgagct
     2041 gccggacgga atatcgtcgg tatacacgtt ctttgaccct gacgtgccgg gcgcaagcta
     2101 tggaacatac aatatattat ggcaggctca cctctgcctc gaactgaagc ttccctacct
     2161 gtacctgggc tactggatca aggacagcgg caagatgtcc tacaaggcgg ggttcaggcc
     2221 gttggaaggt ttgatcgatg ggaaatggga ggcgctgccc gatgtcgatt cctgaagcca
     2281 tctgaaatat cgaacttcgt cgcagtttct cgtcttcggc tcgacgaagg cagggaatct
     2341 acggctttgc cgcccgtatt tccgctgccc gcttatccag ggaaacagcc ttgctcgagt
     2401 atcctatgtc ccgatatagc tgggcaagat tttccagtcc tgtggcgacg ttggggtgat
     2461 ccgggccaaa ggctttttcg gaaatcgcca acgctcggtg gaacagcggt tcagcgaaag
     2521 gatatttccc ctgatccctg tacacgcccg cgaggttgtt caagctcatg gcaacgttag
     2581 ggtgattggg cccgaatgtt ttcttggata tcgagaaaac acgctttagc aagggctctg
     2641 cctcagcata ttttttttgc gccgcataca gtagtgcgag gttgttcagg cttagcgcca
     2701 cctggttatg attcgatcca agcgcctttt ctgaaatggc cagtgcgcgc ttgagaaggg
     2761 gctcggcttt ggaatactgc tccagtgcct gataaagctg cgcgaggtta ttcaggctta
     2821 cagccaaatc cgggtgttcg gatccgagca ccttctcatc aatttcgagt gcgcgtttgt
     2881 aaagcggctc tgcctgcagg taatcgcccc ggtttttgta aagcattccg agattgttaa
     2941 gtgtggcggc gacttcttcg ctttccgttc ccatggattt ttcgaaaatc gcgagtgctc
     3001 gtttgagaag aggttcagcc tttgcatatt gcccctgcgc gttatagagc agggccaggc
     3061 tgttgaggct tgtggccagc gccgggtttt ctgcatccag cgtcttttcg gctgcctcca
     3121 gcgctctctt ggcgacggga atggcgcgag catactctcc ctgctgataa agcgattcaa
     3181 gctcgttatt gagcctgtcc cactcgctca tcgcggcagc aggcacggca atcgaagcga
     3241 gagcggaaat gagcaggaaa ctgatcagct tcataaggat gggtgcgttt gaaagaccgg
     3301 gatgataaac aaaagcgttc ttacattcaa agttttgttc acaatttggt tcgattttta
     3361 tctcgtggac cgtagtttcc agtggtagat tcaacgaggg catgcgggct gttaaaatag
     3421 catatgctgt attcgctgct tcgtccgcta ctattccgtc ttgaccccga gaccgctcat
     3481 cgcctgacat tcgaggcaat cgagaccgcc catcgattcc gattgctaac ccctgcttcg
     3541 gtaccttgcg cttcgcgaac ggtcatggga ctggattttc ccaacccagt gggcctcgcc
     3601 gcgggattgg acaagaacgg cgagcatatc gatgcgctcg cggcgcttgg cttcggtttt
     3661 gtggagatcg gcacagtcac gccgcgtccc cagcccggca atccaaagcc ccggctgttc
     3721 cgtatccccg aggcgaatgc gattatcaac cgcatgggtt tcaacaacct cggtgttgac
     3781 aggttactgg agaatgtcaa gcgagcagcc cccccatgcg tgctgggcat caatattggc
     3841 aagaatttcg acacgcccgt cgataaggcg caggacgatt acctggcatg tctggacaag
     3901 gtttaccgtt tcgcagatta tgtggtggtc aatatctcgt cgcccaacac gcccggtcta
     3961 cgccaattgc aggaggctgg gcagttggat agccttctgt cgggcctcaa ggcggctcag
     4021 cgaaagctcg cagataccca tggcaaatat acgccagtgg cggtgaagat tgcgccagac
     4081 ctggatcccg tgcagatcga atccatcgct gcacttctca tgacacatca aatagatgga
     4141 gtgatcgcga ccaatacgac aatttcgagg gagggcgtgg aaaagctgcc ccattcggag
     4201 gagtccggtg gattgagcgg cgcaccgctc agggatcaat ccaccaaggt tgtgagggat
     4261 ttaaacgtgc ggctcaaggg agcgcttccg atcattgcgg tcggaggtat catgagcccc
     4321 ctggatgcaa aggcaaagat ggatgcgggg gcaagcctgg ttcagattta cagcggacta
     4381 gtttttcgcg ggcctcaact cgtgcaggag atcggaagag cgctttgcaa tcccgatcag
     4441 gcatttcaat attcagcttc gccatgacac gagatcagct tgcagccgca atcgacgcaa
     4501 tcctgccgca gacccagtgc cggcagtgtg gctttccggg ttaccatcct tacgcggccg
     4561 ctattgcgga gggacaggca gacatcaacc agtgtccgcc cggcggagaa gcagcaatcg
     4621 aaaagctcgc ccgcttgctg gggatcagtc cgaaaccgct caatacggcg catggcatcc
     4681 ccaagccgag ggccgttgcg tttatcgatg agcaggcatg cataggttgc acgctttgcg
     4741 cggacgcctg cccggtggat gcaatcgtcg gtgcagcgag gcagatgcac accgtaataa
     4801 ccggcgaatg cacgggttgc gagttgtgta tcgccccatg ccccatggat tgcattacga
     4861 tggtgccggt ccaacttgca gccacgggcc atcctcgcac ggccttggtt accgagcgcg
     4921 gctatccggc agaagaggat gaaaaggaag cggccgatcg cgcacgtgcg cgctaccagt
     4981 tcagattaaa gagactcgag cgcgatcgaa gagaaaaaga agaagcgaag gaggaccgcc
     5041 aaaaggaaaa ggcagaggaa ggtttacttt ccctggagat tgaatcacaa aacttcgagc
     5101 ttgacctgga cgaaaagccg gtgaaggaaa gccaggatcg aaaaagtgcc atgattcggg
     5161 ctgcgctaaa gcgggccatg gctgcacgag cccagcagtt gggtgcggaa gatagaacca
     5221 aa
//
LOCUS       DASSHJ010000213        12234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_911432, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000213 DASSHJ010000000
VERSION     DASSHJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911432"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..726)
                     /locus_tag="VFI43_08360"
     CDS             complement(349..726)
                     /locus_tag="VFI43_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062173.1"
                     /translation="MDDHKIIIRRVVRDEKTGSPEDTVHVFHSSPASRWLLYLLLVPA
                     FIVMAILGIFFFTAFLALFAVVATGLALRLWWLRRELRKPAAGPDHREGEYQVIEDAE
                     IVEEKSTRKNDERQRGEQHKDHP"
     gene            complement(771..2084)
                     /locus_tag="VFI43_08365"
     CDS             complement(771..2084)
                     /locus_tag="VFI43_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET7062174.1"
                     /translation="MVPIKSIVERRQHLALQMQKGVAILPTSPERVRNGDAYYPYRYD
                     SYFYYLSGFTEPEAVLVIVATGDKEASRQILFCRDKDPQQEIWNGFRYGPEAAREAFG
                     FDEAYSVSKLDEMLPKLLVDQPAVFCSFGQSPEWDLRIRGWIHGVRQHARSGVAAPGT
                     INDIRLLLDEMRLFKSPEELQIMRQAAEISAGAHLRAMQQTRPGMNEYQVEADLLQEF
                     RWHGAQAPAYPSIVAGGAKACVLHYIENNAKLDAGDLLLIDAGCELDGYASDITRTFP
                     VDGKFSAAQRDLYGLVLSAQAEAIAAVRPGNSWNAPHEAALRVLAQGFIDFGLCHGTL
                     EQVLQSEEYKRFYMHRTGHWLGLDVHDAGEYKVNGEWRKLCPGMTLTVEPGCYVRPAE
                     DVPEHFWNIGIRIEDDVAVTDDGCEILTSAAPKTVADIEDVMGNR"
     gene            complement(2294..3013)
                     /locus_tag="VFI43_08370"
     CDS             complement(2294..3013)
                     /locus_tag="VFI43_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7062175.1"
                     /translation="MILAAGRGERMKPLTDAVPKPLLRVGGKALIEYHLENLRRAGFR
                     EIVINHAHLGDSIEAFLGNGQRYGLDIRYSREQPALETAGGIANALPLLQPGLEQDGD
                     EQPFLVVNSDIYCQLEFLLLIPTLWNMRRSGDLAYLVLVDNPAHHPAGDFFLDSGRVT
                     LSGGTRLTFSGIGIYKPSLFYGIVPGARVKLAPLLSQAIELGKVGGEHYRGVWMDIGT
                     PERLQQLDRRLAAAVARDSPR"
     gene            complement(3033..4085)
                     /locus_tag="VFI43_08375"
     CDS             complement(3033..4085)
                     /locus_tag="VFI43_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7062176.1"
                     /translation="MERLKHLENWLQAQVAEVPSQFPDQGYSISLASADASFRRYFRV
                     STGKGSLIAMDAPPQHEDCEPFVNVAKLFSAAGVNVPAVLSQNLADGFLLLSDLGNIT
                     YLQALNEAIKNDPAGVTDKADSLYGDAIAALLKIQVASKPGILPEYDEATLLGELNLF
                     PEWYLGRYLQVVPNVEQRAELDKVFSLIVENNLAQPKVFVHRDYHSRNLMVIDPNPGI
                     LDFQDALYGPVTYDLVSLFKDAYIRWDEERILDWLIRYWEQARKLGLPVARDFADFYR
                     DFEWMGVQRHIKVLAIFSRLNYRDGKSSYLNDLPLVEDYVRKACERYRELKPLLTLLD
                     EWQGAKPSDANVGYSF"
     gene            4181..4483
                     /locus_tag="VFI43_08380"
     CDS             4181..4483
                     /locus_tag="VFI43_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HET7062177.1"
                     /translation="MLNQKIVDEVVSKVNELLAQSPVKDVEKNLRVMLSGMFTRLDLV
                     TREEFEVQQEVLKRTREKLTELETKVANLETAAKSQPVSDQIDVPPETLDDLSETE"
     gene            4615..6168
                     /locus_tag="VFI43_08385"
     CDS             4615..6168
                     /locus_tag="VFI43_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET7062178.1"
                     /translation="MPLAILYSRAISGIESPLVTVEVHIANGLPGFTIVGLPEAEVKE
                     SKDRVRAALQNAQFEFPARRITVNLAPADLPKESGRFDLPIALGILAATGQIPADKLD
                     HYEWAGELALTGELRAIRGALAMTWGASRSGRAFVLPRQNAAEAALVREASVYPATSL
                     LQICEHLTGRELLERYDYAEQANGQANGNENGVSGNDWRMPAYVGMDEVKGQGHAKRA
                     LEIAAAGGHSVLMVGPPGTGKSMLAARLPSILPSMTEDEALESAAMQSLGSGGFRISN
                     WKHRPYRSPHHTASGVALVGGGSNPRPGEISLAMNGVLFLDELPEFDRKVLEVLREPL
                     ESGRITISRAARQADFPARFQLIAAMNPCPCGYLGHYNGKCRCTPDQVARYRGKISGP
                     LLDRIDMQIEVAAVSDKELMRVAQGETSATIRERVETAHQRQLQRQGKANSRLAVKEI
                     DEYCIPDNGAATLLKQAISKLNFSARAYHRILKVARTIADLAESNAITSTHVAEAVQY
                     RKLDKNQRA"
     gene            6314..8242
                     /locus_tag="VFI43_08390"
     CDS             6314..8242
                     /locus_tag="VFI43_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7062179.1"
                     /translation="MASADLQTDNQSAKTYDFVVVGGGVAGLYCCNELIKKSRSGALN
                     IKSILLLEASDRFGGRIETWSLLRSDEQNGKATGFEDTWDPCSWDFTQPDRNPLAPEG
                     NKKSPFLGPQETFARIDEKGNVKTREVEYFRAEFGPMRIEPRDQPLLQALLDELGIRE
                     QVPGAKPSLDDLIPFSSYASEEPPEPRFTLQGEEANQETPLDLMILALRRILELVDLD
                     NMEAPWETSDANEQWRNLHDDAFFYRHYWKGELLEWIHNLTEADFCKIREHACFRGQP
                     LWNLGFWNVLSDVLSHFAVVKIRDWGSYYHFVHENLNAAEWIIFWLRAIRSTGSLRGI
                     RGGMSRAIWKLLEKLYAEENRALLTIRKGAKVSGLRSYGNTVEVIVDQSDEVIRARKV
                     ILALPKRPLQELSWNGTGAPKKLQEALNGVVGLPLLKCFFVIEKPWWEDNRPLNRNAA
                     DLPTRELLYMKSDDRTKGLIMIYTDRPAITFWSDYLRMEPTQDASGQKLEGPQIEYQE
                     TARTWFLKKFPAPFTQGKPPPINSPALVKEPGSPRLWQRFVQFARDYGHHDFTKDRLL
                     ACGMRDWGKKPFGAAAHGWLPGIMPWKHMEFLEAFSLDEDSPEMNIHICGEAFSDYQG
                     FIEGSLRSAKRVLNRIAP"
     gene            8367..12098
                     /gene="metH"
                     /locus_tag="VFI43_08395"
     CDS             8367..12098
                     /gene="metH"
                     /locus_tag="VFI43_08395"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET7062180.1"
                     /translation="MNENSRAQLLEHLLEQRILLLDGAMGTMIQSHKLSESDYRGTRF
                     ADFPHDLKGNNDLLTLTQPQIIRSIHDAYLEAGADIIETNTFNSTSASMSDYHMQDLV
                     YELNFAGAQLASKAAQAMEAKTPEKPRFVAGVLGPTTKTTSISPDVNDPGFRSITFDQ
                     LVADYSVSIRGLIDGGADILLVETIFDTLNAKAALFAIDQYFEAHGKRVPIMISVTIT
                     DASGRTLSGQTPEAFWDSVQHARPLSVGLNCALGAELMRPYIEELSRVAEVYVSAHPN
                     AGLPNPLAETGYDESPSYTAGLIKGFAQSGFVNIVGGCCGTTPAHIKAIADSVSGIAP
                     RKIPDIPKKLRLSGLEPLNIGDDSLFVNVGERTNVTGSKAFARLILGNDYAQALSVAR
                     SQVESGAQIIDVNMDEAMLDSQKAMVTFLNLVAAEPDISKVPVMIDSSKWSVIEAGLK
                     CVQGKSIINSISLKEGEAEFIEHAKLARRYGAAVIVMAFDDQGQADTLKRKVDICTRS
                     YRILVDQVGFPPEDIIFDPNIFAVATGIEEHNNYGVDFIDATRIIKQTLPHAKVSGGV
                     SNVSFSFRGNEPVREAIHTAFLYHAVKAGMTMGIVNAGQLGVYSDIPKDLLERVEDVL
                     LNRTPKDSSLSPTERLVEFAETFKGKSKEAAEDLSWREGTVQERLTHALVKGITTYIV
                     EDTEAARLEIMKEGGRPIQVIEGPLMNGMNVVGDLFGSGKMFLPQVVKSARVMKQAVA
                     HLLPFIEAEKKLSGDLRPKGKIVIATVKGDVHDIGKNIVTVVLQCNNYEVINMGVMVP
                     STQILEMARKENADMIGLSGLITPSLEEMAHVAKEMEREGFTIPLLIGGATTSRVHTA
                     VKVAPNYSGVTVWVPDASRAVGVCSNLLSEDLREGYVRELAAEYERVRTQHKAKKGPA
                     PMLSIAEARVNAFKTDWNTYTPPEPDFIGVRSLRNYPLEKIVPFIDWTPFFQAWELSG
                     RFPAILEDEVVGEAASNLYRDAQAMLKKIVEQKWLGANAVFGLFPANRVNADDIEIYA
                     DKGRKRVAMTYHSLRQQTTKPGDRPNLCLADFIAPQETGIRDYIGAFAVTAGLGIDAR
                     IKAFEEVHDDYSAIILKALADRLAEAFAEHMHWRVRREFWGFAKDENLNSDQLVNEEY
                     RGIRPAPGYPACPDHTEKGPLFELIKAPENSGIIITESYAMVPTAAVSGFYFSHPEAD
                     YFAVGKIAKDQVEDYAKRKSWTLEEAERWLAPVLAYERQ"
BASE COUNT         2887 a         3317 c         3414 g         2616 t
ORIGIN      
        1 cgacagccat tcctaccggg ccgccgccga tgatgatgag gtcgtaatcg taatgatgcg
       61 tgctcattgt gcttctacat cctgcccttc tgtggatcag tattttccag ccacccctct
      121 cagctgacga actgtttttg cgaggtcgac tgcctgttca atgcagccaa gccgcttaaa
      181 actgcttccc tctccctcca gagagagagc agggagaggc cgcgcaatcc tccctctctc
      241 ccagcctctc tcccaagaat gggagagagg agttgacgtg cttgggtcac aggcgtcagc
      301 tgaggtcctc tccgcgtagg accagaagtc tctccaagta tgaatcattc acggatgatc
      361 tttatgctgc tccccacgtt gccgctcatc attttttctc gtgctttttt cctctacaat
      421 ttcggcgtct tcgatcacct ggtattcccc ttcgcggtga tccggtcctg cagcaggctt
      481 gcgcaattca cgccgcagcc accacaaccg taatgcaagg ccggtggcaa ccaccgcaaa
      541 caacgctaga aaagcggtga aaaagaaaat gcccaggatg gccatgacga tgaacgctgg
      601 aaccaggagc agataaagga gccagcggct cgccgggctc gaatgaaaaa catgcacggt
      661 atcttccggc gagcccgttt tctcgtcccg cactacccgc cttatgatga ttttatgatc
      721 gtccattgag tcaggcctcc ctgtctgcgt gtctgcgcgt cggtctacct ttaccggttt
      781 cccatgacat cttcaatatc ggccacagtc tttggcgctg ccgaggtgag aatttcgcag
      841 ccatcatccg taaccgccac atcatcctcg atgcgtattc ctatattcca gaaatgctct
      901 gggacgtcct cggccgggcg cacatagcac cccggctcca cggtaagcgt catccccggg
      961 catagcttcc gccattcgcc gtttaccttg tactcgcctg cgtcatgtac gtccagtccc
     1021 agccagtgac cggtgcggtg catataaaac cgcttgtatt cttcagattg caaaacctgt
     1081 tcgagcgtgc cgtgacataa gccaaaatca atgaagcctt gcgcaagcac gcgcagagca
     1141 gcctcatgtg gagcgttcca gctgttcccc gggcgcaccg ccgctatcgc ttctgcctgt
     1201 gcggaaagca cgagcccgta taggtccctt tgggcagcgc tgaactttcc gtccacggga
     1261 aaggtgcggg taatatcgga tgcatacccg tccagttcac atccggcatc gatcagcaac
     1321 aaatccccgg cgtcgagctt cgcattgttt tcaatgtagt gcagtacgca ggctttggct
     1381 ccccctgcga cgatagaggg gtacgcagga gcctgtgcgc cgtgccagcg gaattcctgg
     1441 agcagatctg cctcgacttg atactcattc attccggggc gggtttgctg catggcccgc
     1501 aagtgggcgc ccgcagaaat ttcagctgcc tggcgcatga tctgtagttc ttcggggctc
     1561 ttgaacaaac gcatttcgtc cagcagaagc cggatatcat tgattgttcc tggcgcagcc
     1621 acgccgctgc gggcgtgttg gcgtacgcca tggatccagc ctctaatcct caggtcccac
     1681 tccggggact gcccaaagga gcagaagact gcaggctggt ccacgaggag ctttggaagc
     1741 atctcatcca gtttcgagac agagtaggcc tcatcgaatc cgaaggcttc ccgtgctgcc
     1801 tccggcccgt agcgaaatcc attccatatc tcctgttggg ggtccttgtc acgacagaaa
     1861 aggatttgcc tggaagcttc cttatcacct gtcgccacaa ttaccaatac tgcctcgggc
     1921 tcggtaaagc cgctcaggta ataaaaatag ctatcgtagc gatagggata ataggcgtct
     1981 ccgtttcgca ccctttccgg cgatgtaggg agaatggcaa cgcctttttg catttgtaat
     2041 gccaaatgct ggcgccgctc tacgatagat tttatcggga ccatggatca ggatttttgc
     2101 agtatttgca gaaaatggca aagaaagcac ggaaattctc gtgggggcga attacgagaa
     2161 agtttcgggg gaatctatcg agggaattta aaatgaggaa attgaaatcg gaagtgccgt
     2221 tgaaaaaaat gaaaggtgaa ggctaaaggc aattcaattg agaattcttc agcgcttcat
     2281 ccgctatgtg gggctatcgg gggctatcgc gggcaaccgc cgcggcaagc cgacggtcaa
     2341 gttgctgcaa gcgctcgggc gttccaatgt ccatccatac gcctcgataa tgctccccgc
     2401 ccactttccc caattcgatt gcctggctca acagcggcgc aagcttgacc ctcgctcctg
     2461 gaacaatacc gtagaaaagg ctcggcttgt aaataccgat gccgctgaaa gtcagccggg
     2521 ttccgccaga aagtgtaact ctgcctgaat caaggaaaaa atcacctgcc ggatgatgag
     2581 caggattgtc gaccagcacc agatatgcga gatctccgct ccggcgcata ttccaaaggg
     2641 tgggaatcag gagcaaaaat tccagctggc agtagatatc cgaattgaca accagaaacg
     2701 gttgttcatc gccgtcctgc tccagcccag gctggagcaa aggcagggcg tttgcgatac
     2761 ctcccgccgt ttccaaggcc ggctgctcgc gcgaataacg tatatcgagg ccgtaacgct
     2821 ggccattacc taggaaagct tcgatcgaat cgcccaggtg agcgtgattg atcacgattt
     2881 cgcgaaaacc cgcgcgccgg agattttcca gatggtattc gatcagcgcc ttgccgccca
     2941 cccgaagcaa gggttttggc acagcatcag tcaagggctt catgcgttcg cccctgccgg
     3001 cggcaaggat cattgccctg aatggcagtt tctcagaaag agtagccaac gttcgcatct
     3061 gaaggcttcg cgccttgcca ttcatccagg agcgtaagca gcggcttgag ctcgcggtag
     3121 cgctcgcagg ccttgcgcac gtaatcctct accagaggga ggtcgttaag ataactgctt
     3181 ttgccgtcgc gatagttcag gcgtgagaat atcgcgagaa cctttatgtg gcgctgcacg
     3241 cccatccact cgaagtcgcg gtagaagtcg gcaaaatccc gcgccacggg aagcccgagc
     3301 tttcgcgcct gctcccaata gcggatcagc cagtctagta tccgctcctc gtcccaccgg
     3361 atgtacgcgt ccttgaatag cgataccaga tcgtacgtca ccggaccgta aagcgcgtcc
     3421 tggaaatcca ggatgccggg attgggatcg atgaccatca ggttgcgcga gtggtagtcg
     3481 cggtgcacga agaccttggg ttgtgcgaga ttgttttcga cgatcaggct gaaaaccttg
     3541 tcaagctctg ctctctgttc cacattcggc acaacctgca aatatctccc aagataccat
     3601 tccgggaaca ggttcaattc gcctagaagc gtcgcctcat cgtattcagg aagaatgccg
     3661 ggcttgctcg ccacctggat cttgaggaga gcagcaattg catccccgta gagactgtct
     3721 gccttgtctg tcacgcccgc tggatcgttc tttatcgcct cattcaacgc ctggagataa
     3781 gtgatgtttc caagatcgga gaggagcagg aacccatccg cgaggttttg ggacagcacg
     3841 gccggaacat tcactcccgc cgcgctgaac aattttgcca cattcacaaa cggttcgcag
     3901 tcttcgtgct gcggtggagc atccatggcg atgaggcttc ctttgccggt gcttacccgg
     3961 aagtagcgcc gaaagcttgc atcagcggat gcaagtgaaa tggaatagcc ttggtcggga
     4021 aattgcgaag ggacttcagc cacctgtgcc tggagccagt tctccagatg cttcaaacgt
     4081 tccataaagc cgattgccta atttgatgaa gtatgagatt ttatcatgcg tacattatgc
     4141 tcttccgatc caatacccaa tgagattcaa ggaggaaatc atgcttaatc aaaaaatcgt
     4201 ggacgaggtg gtaagcaagg tcaacgaact gctggcccaa agtccggtaa aggatgtgga
     4261 aaagaatctc cgggtgatgt tgtctggaat gttcacccga ctggatcttg taacgcgcga
     4321 agaattcgag gtacagcagg aggtgctgaa gcgcacgcgc gagaagctga ctgaactgga
     4381 aacgaaggtg gccaacctgg aaacagcggc aaaatcacag cccgtttccg atcagatcga
     4441 cgttccgcca gaaacactgg atgacctgtc ggaaacggaa taacaagttt gttagcggat
     4501 ttacgagaac ctcctgttgt taatcgttcg atacctttgc gaaatatcgg agtatcaccc
     4561 gctgatcgtt cttcatcagt aatcggtcat cctgtttgct tgatcagttt ttgaatgccc
     4621 ctagcgattc tttatagccg cgccatttcg ggcatagagt cgccgctggt gacagtggaa
     4681 gttcacattg ccaacgggct cccaggtttt accattgtcg gcttgcccga agctgaggtg
     4741 aaggaaagca aggacagggt tcgtgctgcg ctccagaacg cgcaattcga gtttccagcg
     4801 cggcggatca ccgttaacct tgcgcccgcg gatttgccaa aggaaagcgg gcgcttcgat
     4861 ctgccgatcg ctttggggat actcgcggcg acgggacaga ttcctgccga caagcttgac
     4921 cactacgaat gggcgggtga actggcacta acgggcgagc ttcgcgccat ccggggagca
     4981 ttggcgatga cctggggtgc ctcgcggtca ggacgcgcgt tcgtgctgcc tcgccaaaat
     5041 gccgctgagg ccgcgctggt gagggaggcc tcagtttacc ctgccacctc cctgttgcaa
     5101 atttgtgagc acctcaccgg acgcgaactg ctggagcgat atgattacgc agagcaggca
     5161 aacgggcaag caaacggaaa tgaaaatgga gtttcgggca acgattggag aatgcctgcc
     5221 tatgtaggca tggacgaggt aaaagggcag gggcatgcga agcgtgcgct agagattgcc
     5281 gcggcaggcg gtcacagcgt gttgatggtc gggccgcccg gtactggcaa atccatgctc
     5341 gccgcgcgcc tgccgagcat tctgccttcg atgacggaag atgaagccct cgaatccgcc
     5401 gccatgcagt cgctgggtag cggaggattc aggatatcga actggaagca ccgcccttac
     5461 cgctcgcccc atcacacggc ttcaggagtg gcgctggtgg ggggcggaag caatccccgt
     5521 cccggcgaga tatcgcttgc aatgaatggg gtgctgtttc tggacgaact gcccgaattc
     5581 gaccgtaaag tgctggaggt gctgcgggag cctctggaat cagggcggat cacaatttcg
     5641 cgcgctgcac gccaggcaga ttttcctgcc cggttccagc ttattgctgc catgaatccc
     5701 tgcccttgtg gctacctggg gcattacaac gggaaatgcc gctgtacccc ggatcaggta
     5761 gcccgctatc gtgggaagat atcaggcccg ctcttggatc gtatcgacat gcagatcgaa
     5821 gtggccgccg tatcagataa ggaactgatg cgggtggctc agggtgaaac aagcgcgaca
     5881 attcgcgaac gtgtggagac ggcacatcag cgccaactgc aacggcaggg aaaggcaaac
     5941 tcccggttgg cggtcaagga aatcgatgaa tattgcattc ccgataatgg cgcggcaacc
     6001 cttttgaagc aggcgatcag caagctgaat ttttcagcgc gtgcttatca ccgaatcctg
     6061 aaagtcgcgc ggaccatcgc cgatcttgcc gaaagcaatg caattaccag tactcatgtt
     6121 gccgaagcag tgcagtatcg caaactggat aaaaaccagc gagcgtgatg actgggcatt
     6181 atttccactc gaaactggac atgaccgcgg caagacggag cagttggtga aacgtaaagc
     6241 gctaacgaaa gcgttagcat tcgtcttgtg ctgaaacgat tttttttcaa agaccctcgc
     6301 gtttgaaaag aacatggcct ctgcagatct ccaaaccgat aatcagtctg cgaaaactta
     6361 cgacttcgtg gttgtcggcg ggggagtcgc cggcctgtat tgctgcaacg aactcatcaa
     6421 gaaaagcaga agcggtgcgc tcaacatcaa gagcattctc ctcctggagg catctgatcg
     6481 attcggcgga cgcattgaaa cctggtcgct tctgagaagc gacgagcaga acggaaaagc
     6541 gactgggttc gaggacacat gggatccttg cagctgggat ttcacgcaac cggatcgaaa
     6601 tccacttgcc cctgaaggga acaagaaatc accctttctc ggcccacagg aaacctttgc
     6661 ccggatcgat gaaaaaggca acgtaaagac gcgggaggtt gagtatttcc gggccgagtt
     6721 cgggcccatg cggatagagc cgcgggacca acctttgttg caggctctgc ttgatgaact
     6781 cggaatcagg gagcaggttc caggcgcgaa acccagcctt gatgatttga taccattttc
     6841 gtcctacgca tcggaagagc cccctgagcc ccggttcacc ctgcagggag aagaggcaaa
     6901 ccaggaaacg cccctcgatc tgatgatcct cgccctcagg cggatcctgg aactcgtcga
     6961 tctggataac atggaagcac catgggaaac cagcgatgcc aacgaacaat ggagaaatct
     7021 ccacgatgac gcgtttttct atcgccatta ctggaagggg gagttgctgg agtggatcca
     7081 caatctgacc gaagcggatt tctgcaaaat ccgggagcat gcatgcttcc ggggtcagcc
     7141 actttggaac ttgggtttct ggaatgtgct aagcgacgtt ctaagccatt tcgctgtggt
     7201 gaaaatcagg gattggggaa gctattacca tttcgtgcac gaaaacctga acgccgccga
     7261 atggatcatc ttctggctga gggcgattcg cagcacggga tcgcttcgag gcatcagggg
     7321 cgggatgagt cgagcgatat ggaaacttct cgaaaagctt tatgcagaag aaaacagggc
     7381 gcttctaacc atccggaaag gcgcgaaggt gtcaggtctt cgctcatatg gaaatactgt
     7441 cgaggtgatt gtcgatcaat ccgatgaagt catccgtgcg cgaaaagtta ttctggcatt
     7501 gcccaagcgc ccactgcagg aattatcatg gaatggaaca ggggctccaa aaaaactgca
     7561 ggaagcgctg aatggcgtgg ttggtctgcc gttattgaag tgcttctttg tgattgaaaa
     7621 gccctggtgg gaagataaca ggcctctcaa tcggaatgcc gcggatttgc ccacgcggga
     7681 gctgctttac atgaaaagtg acgaccggac caaagggctg atcatgatct ataccgatcg
     7741 gccagccatc actttctggt ccgattacct gagaatggaa ccgacgcagg atgcctctgg
     7801 tcagaaattg gaagggccac agattgagta tcaggaaaca gcccggacgt ggtttctgaa
     7861 aaagtttcct gcccctttca cccagggcaa gccaccgcca ataaacagtc ctgcgcttgt
     7921 gaaagaaccc ggttcccccc ggttatggca acgttttgtg cagtttgcgc gagattacgg
     7981 gcatcacgac tttacgaagg accgtctgct tgcttgtggg atgcgtgact gggggaaaaa
     8041 accttttggc gcagctgcgc acggatggct gcctggcatc atgccctgga agcacatgga
     8101 gtttctggag gctttttccc ttgacgagga cagccctgaa atgaatatcc atatctgcgg
     8161 cgaagctttt tccgactacc agggctttat cgagggatca cttcgctccg caaaacgcgt
     8221 cctgaacagg attgcccctt agtaagccaa aaagtaagcc aaaaggcaaa cggcaccttc
     8281 agcacgcgct ctccgtccct gtttcacttc cataacgcaa ctgaggagcg tgctagaatt
     8341 cacgcctcct cacctatacc ttaatcatga acgaaaattc tcgcgcgcaa ctgctggaac
     8401 atctccttga acagcgcatt ctgctgctgg acggcgccat gggtaccatg atccagagcc
     8461 acaagctcag cgagagcgat tatcggggaa cccgctttgc cgattttccc catgatctca
     8521 agggtaacaa tgatctgctg acgctcacgc agccccagat catccgatcg atccacgatg
     8581 cttacctgga ggcaggcgcc gacattatcg aaaccaacac gtttaattcc acgtctgcct
     8641 cgatgtcgga ttaccacatg caggacctgg tgtacgagtt gaattttgcc ggcgcacaac
     8701 tcgccagcaa agcagcccaa gcgatggaag cgaagacgcc tgagaagcct cgctttgtgg
     8761 ctggcgtgct tggcccgacc acgaaaacca cctcgatctc gccagacgtg aacgatcccg
     8821 gttttcgcag catcacgttc gaccagctgg tggcggatta ttcggtatcg attcgcgggc
     8881 tgatagatgg tggagcggac atccttctgg ttgagacgat attcgacacg cttaatgcga
     8941 aggcagccct cttcgccatt gaccagtatt tcgaggcgca cggcaaacgc gttcccatca
     9001 tgatttcggt gaccatcacc gacgcctcag gacgcacgct ctcggggcag acgccggagg
     9061 ccttctggga ttccgtgcag cacgcccgcc ccctgtccgt cggactcaac tgcgctttag
     9121 gcgctgagct catgcggcct tatatcgaag agctttcccg ggtggccgag gtctatgtca
     9181 gtgcccatcc aaacgcgggc ctgccgaacc ccttggccga gaccgggtat gacgaatcgc
     9241 cttcctatac cgctggcctt atcaaaggct ttgcgcaatc aggctttgtc aacatcgtgg
     9301 gcggctgctg cggaacgacc cccgcgcaca tcaaagcgat agccgactct gtgagtggca
     9361 tcgcgccaag aaaaattccc gatattccca agaaacttcg cctctcgggg ctggagccgc
     9421 tcaacatagg cgacgactcc ctgttcgtga atgtcggcga gagaacgaat gttacgggct
     9481 cgaaagcgtt cgcccgcctg atactcggaa acgattacgc gcaagcgctg agcgtcgcgc
     9541 gcagccaagt ggagagtggg gcgcagatca tcgatgtgaa tatggacgag gcgatgctgg
     9601 actcgcaaaa ggcaatggtc accttcctga acctggttgc cgccgagccg gatataagca
     9661 aagtgccggt catgatcgat tcgagcaaat ggtcggtgat cgaagcggga ctgaagtgcg
     9721 tacagggcaa atcgatcatc aattcgatca gcttgaagga aggtgaggcg gaattcatcg
     9781 agcatgcgaa gctggcacgg cgctacggcg cggcagtgat tgtgatggcg ttcgatgacc
     9841 aggggcaggc cgatacgttg aagcgcaagg tggatatttg cacgcgctcc tatcgaatcc
     9901 tcgtcgatca ggtcggcttt ccgcctgagg acatcatttt cgaccccaat atcttcgctg
     9961 tcgctaccgg tatcgaggag cacaataatt acggtgtgga ttttatcgac gcgacccgca
    10021 tcatcaagca aaccctgccg catgccaagg tcagcggagg cgtctccaac gtatcctttt
    10081 cgttccgggg caatgagccc gtgcgcgaag caatccatac agccttcctc taccatgccg
    10141 taaaagcagg aatgacgatg ggcatcgtaa atgccgggca attaggcgtt tattccgata
    10201 ttcccaagga tttactcgaa cgggtggagg acgtgctgct caaccggacg ccgaaggata
    10261 gcagtttaag cccgaccgag cggctggtgg agttcgcgga aaccttcaag gggaagagca
    10321 aggaagcagc ggaagacctt tcctggcgcg aaggaaccgt gcaagaacgt ttaacccatg
    10381 cattggtcaa gggcattacg acctatatcg tggaggatac ggaagcggcc cggctggaga
    10441 tcatgaagga agggggccgg cccatccagg ttatcgaagg gccgctgatg aatggaatga
    10501 acgtggtagg cgatctgttc ggctcgggca agatgttcct gccccaggtt gtcaagtcgg
    10561 cacgcgtgat gaagcaggcc gtcgctcacc tcttgccgtt tatcgaggct gaaaaaaagc
    10621 tgtcgggtga tttgaggcca aagggcaaga tcgttattgc caccgtgaaa ggggatgtgc
    10681 atgacattgg taaaaacatc gtcacagtag tgctccagtg caacaactat gaagtcatca
    10741 acatgggggt tatggttccg agcacgcaga tactcgagat ggcgcgcaag gaaaatgctg
    10801 acatgattgg cctttcaggc ctgattacgc cctcactgga agaaatggcc catgtggcaa
    10861 aggaaatgga gcgcgaaggt tttaccattc ctttgctgat cggcggggcg accacttcac
    10921 gcgtccatac agcagtgaaa gtcgcgccaa attatagcgg cgtcacggtg tgggtgccgg
    10981 acgcgagccg cgccgtcggt gtctgcagca acctgctttc ggaagatttg cgggaaggtt
    11041 acgttcgcga actcgccgcg gaatacgaac gggtccggac ccagcataaa gcgaaaaaag
    11101 gacccgctcc aatgctttcc atagcagaag cacgagtaaa tgcttttaaa acagactgga
    11161 atacctatac tcctcccgag ccggatttta tcggggtgag atcactgcgg aattatccgt
    11221 tggaaaaaat tgtgcccttc attgactgga cccctttctt ccaggcgtgg gagctatccg
    11281 ggcgttttcc ggcaatcctg gaagatgaag tcgtcggcga agcggcgagc aatttatacc
    11341 gggatgcgca ggcgatgctc aagaagatcg tcgaacaaaa atggctcgga gcaaacgccg
    11401 tattcggcct gttccccgcg aacagggtga atgccgacga cattgagatc tatgcggaca
    11461 aagggcgtaa aagagtcgcg atgacttacc atagcctgcg tcagcaaacg accaagcccg
    11521 gggaccgccc gaatctatgt cttgcggatt tcatcgcacc gcaagagacc gggatccgtg
    11581 attacatcgg cgccttcgcc gtaactgcgg gactggggat tgatgcccgc atcaaggctt
    11641 tcgaggaagt gcacgatgac tatagcgcga tcatcctgaa ggccttggca gaccggctgg
    11701 cggaggcctt cgccgagcat atgcactggc gggtccgccg cgaattctgg ggttttgcaa
    11761 aggatgaaaa tctcaacagc gaccaactgg ttaacgaaga ataccgcgga attcgtcctg
    11821 ccccaggtta cccggcctgc cccgatcaca ctgagaaagg cccattgttc gagctgatca
    11881 aggctcctga aaactcaggc atcatcatca ccgagtcgta cgccatggtt cctaccgcag
    11941 cagtttccgg cttctatttt tcccacccgg aagccgacta ttttgcagtg ggcaagattg
    12001 ccaaggatca ggtagaggat tacgcaaaac gcaaaagctg gacgctcgaa gaagcggaaa
    12061 ggtggcttgc gccggtactg gcctacgagc ggcagtagga ggtcaagtac tctcgcctca
    12121 actgcccgca aacggacatc cagggaaaac ccgctcgctg tacgctaagc cgtttaaagc
    12181 tgtctagact ccctcccgtt agccggagag ggcaaaaatg cctgcattca gtag
//
LOCUS       DASSHJ010000214         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_927433, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000214 DASSHJ010000000
VERSION     DASSHJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927433"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VFI43_08400"
     CDS             complement(<1..1297)
                     /locus_tag="VFI43_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET7062181.1"
                     /translation="MSLKWKVLLLSSLILIAIVVSFTGITYRSLMNDFEKQREVQHRR
                     YALEVEGLIQQISNNLHQLAQLIPFLEGMSAALLSSRAALINQIFDSYWAPLQLNNDI
                     ELIRFYGSSSMLLASWGVSESYTEESVMRTWVQEANLREQPISPLSCDKSCTQFTVTP
                     LLVEGKHVGVVVIGVPLVDVVLGFKDISGAHIGLLMKEQGSVPGPGNNGAKIENWNFQ
                     LTAHSKEMNVEVLQRAAELYPDFDSIKDGVDVFWDNRYLRIKPLALHELIVSEKARLV
                     VITDISSTIRTIQRSTQQSIIIGLVGLILSEILLFIILTKPLSRLKHIVFTLPLLARS
                     SFHDFRSSLRSTTQKQLLKDEIDMLDETAVALSFQLETLEDQVADRTRILARKMDELS
                     KERDFITHLLDIAQVIVITQNAQGEILTLNAHGEMLIQYA"
     gene            complement(1373..2608)
                     /locus_tag="VFI43_08405"
     CDS             complement(1373..2608)
                     /locus_tag="VFI43_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7062182.1"
                     /translation="MCSRSSNGALARFLQSAGWVAVAVIACTNIAHAQKLPIPQNLPQ
                     NLQIHGFASQSWLKSSENNVFGKSSSDIGSFDFRELGINASMRPLSNLQISVQGISRW
                     AGKGSPGNFRLDYGFIDYTFLSGESSQLGIRLGRMKNPLGFYNDTRDVPFTRPSILLP
                     QSIYFDRTRKLALAADGVHLYGEYRSDLGDISFQGGAVRPLVRGLEAEAAVFGTQLQG
                     HLTPDMSYVGRINYELDGGRFRLAVSATELNMGFSPAVGDKVSEGMIRFIPLVFSAQY
                     NAERWSLTSEYALRNLKYDFMTGPNMHFTGESYYFQGAYRFTPEWEGFVRYDVLYTDR
                     DDRNGKEYAATSHRPAYTRFAKDLTAGVRWNITPEFMVRAEYHRVNGTAWLSILDNPS
                     ISDQAQHWDLFSILGSYRF"
     gene            complement(2589..3059)
                     /locus_tag="VFI43_08410"
     CDS             complement(2589..3059)
                     /locus_tag="VFI43_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062183.1"
                     /translation="MGTKKLVVATRIIIGRTFFLLWLLFGSIFAARTACANEPYEVVT
                     NPSVSEKTISKSSLRAIFGMRLHAWPDGSAISVFVMPDDAPLHAAFSKEKLNVFPYQL
                     RSAWDRLVFSGTGQAPDTVASTDEMLAKVASTPGAIGYLPKSKIDGRVHVLQIK"
     gene            complement(3167..3961)
                     /locus_tag="VFI43_08415"
     CDS             complement(3167..3961)
                     /locus_tag="VFI43_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HET7062184.1"
                     /translation="MNDVVAAFHEYFEITNVNSVELLREVFNLRYRVLCIEQRAPGFD
                     ASSYPDEMESDNYDRHSSHILLRHRPSGVFVGTARMILPDPLHLNKLFPTERHMRFDP
                     ALLNTARLPRKQTGEISRLVVIRRFNRRKDELIDALENGRNIEKWSPTVKRRFPHPML
                     ALAVGIIRMSVDNNINHWLSVMEPALNRLLSLYGLHLDPVGPLIDHHGLRRPYYADFP
                     HMLDRMHRNHRQFWELVTDGGKVQPSITQRPCEGLLCEATQGEALE"
BASE COUNT         1142 a         1189 c         1312 g         1149 t
ORIGIN      
        1 ccgcgtactg gataagcatt tccccatgcg cgttcagcgt aaggatctcc ccttgggcat
       61 tctgggtaat gacgattacc tgtgcaatat ccaggaggtg cgtaatgaaa tcacgctcct
      121 tgctcaattc atccattttg cgcgccagga tccgggttcg atccgccacc tgatcttcaa
      181 gcgtttcgag ctggaaggac aatgccacag ctgtttcatc cagcatatcg atttcgtctt
      241 tgagcaattg tttttgcgtg gtggaacgaa gcgacgaacg aaaatcatga aaactgctgc
      301 gggccagcag cggcaacgtg aaaacgatat gcttgagtcg cgataaaggc tttgtgagaa
      361 tgatgaataa caatatttca gagagtatga gtccgacaag gcctatgatg atgctctgct
      421 gggtcgaacg ctggatcgtg cgaatagtcg aactgatatc cgtaatgacg accagccttg
      481 ccttctcgga cactattagt tcgtgcagcg caaggggttt gattcgcaga taccggttgt
      541 cccagaaaac gtcgactccg tccttgatgc tgtcgaaatc ggggtagagc tcagctgccc
      601 gctggagaac ttcgacgttc atctccttgc tgtgcgcggt gagctggaaa ttccagtttt
      661 caattttcgc gccgttatta ccaggaccag gcacgcttcc ctgttctttc ataagcaacc
      721 cgatatgcgc gcctgaaatg tccttgaagc ccagcacaac gtccaccaat ggaactccga
      781 tcactacaac gccgacatgt tttccttcaa cgagcagagg cgtgaccgtg aattgggtgc
      841 agcttttgtc gcaactcagc ggactgatag gctgctcgcg gaggtttgct tcctgaaccc
      901 aagtgcgcat aacagactct tcggtatacg attcagaaac accccagctt gccagcagca
      961 tgctcgaact gccataaaac cggattagtt cgatatcgtt gttaagctgc aggggcgccc
     1021 agtagctgtc gaaaatctga ttgatgagtg ctgccctgct tgagagcagg gcagcactca
     1081 tcccttcaag gaaagggatg agttgggcga gttgatgcag gttgtttgaa atctgctgta
     1141 tcagtccttc cacctcgaga gcgtagcgtc gatgctgcac ttctctctgc ttctcaaagt
     1201 cgttcatcaa actccggtaa gtgatccccg tgaacgagac gacaatagcg atcaagatga
     1261 ggctgctgag cagcaagacc ttccatttca ggctcaagaa tttccggtcg gctttttcag
     1321 cgtcttgaag ctgggactcg cttttatgga gattcctttc cattaggcga agttagaacc
     1381 ggtaggagcc aagaatggaa aagagatccc agtgttgagc ctggtcagat atggaggggt
     1441 tatccagaat tgagagccag gcagtgccgt tcacccggtg gtattctgct cgcaccatga
     1501 attccggggt gatattccaa cgcacaccgg cggtgagatc ctttgcaaac cgggtatatg
     1561 cgggacgatg ggaagtcgca gcgtactcct ttccgttgcg atcgtccctg tcagtataaa
     1621 ggacgtcgta gcggacgaag ccttcccact cgggcgtgaa acggtacgcc ccctgaaagt
     1681 agtagctttc tcccgtaaaa tgcatgttgg gccctgtcat gaagtcgtat ttcaaatttc
     1741 gcagcgcgta ttcggacgtg aggctccagc gttctgcatt atattgggcc gagaaaacca
     1801 gggggatgaa ccgaatcata ccctctgaga ccttatcgcc gacagcgggt gagaatccca
     1861 tattcagttc tgtggcgctg accgcaagac ggaaacgccc tccgtccaac tcgtagttta
     1921 tccggcctac ataggacata tcgggggtaa ggtgaccttg aagttgcgtc ccaaaaactg
     1981 cggcttccgc ctctaaacct cgaacgagag gacgtaccgc ccccccctgg aaggaaatat
     2041 cgcccaggtc agagcggtat tctccgtata ggtgcacccc atccgcagcg agcgcgagct
     2101 tgcgcgtgcg gtcgaaatag atcgactgag gcagcaatat gcttggcctt gtgaagggga
     2161 cgtcgcgcgt gtcattatag aaaccaagcg gatttttcat cctgcccagc cgaatcccta
     2221 gctgactgga ttcacccgat agaaaggtgt aatcgataaa tccgtaatcg aggcggaagt
     2281 tgccggggct tccttttccc gcccatcgcg aaatcccctg taccgagatc tgcaggttgg
     2341 aaagaggcct catggaggcg ttgataccca gttcccggaa atcaaagctc ccgatgtccg
     2401 aactgctttt tccgaaaaca ttattttctg aactcttcag ccaggactga ctggcaaaac
     2461 cgtggatttg caggttctgg ggcaggttct gggggatcgg cagtttttgc gcgtgcgcga
     2521 tgttagtgca agcgatgacc gcaacggcaa cccatccggc agactgaagg aaacgggcga
     2581 gggcgccgtt acttgatctg gagcacatgg actctgccat ctattttgga tttgggtaaa
     2641 tagccgatgg ctcccggcgt gctggcaacc ttggcgagca tttcgtcggt agatgccaca
     2701 gtatctgggg cctggcctgt cccggaaaaa accagtctgt cccaggccga acgcaactga
     2761 tacggaaaaa cgttgagttt ttccttcgaa aatgcagcat ggagcggtgc atcatctggc
     2821 atcacgaaca cgctaatggc gctcccgtct ggccaagcgt ggaggcgcat cccgaatatg
     2881 gcgcgtagcg aacttttgga tatcgttttt tctgaaacgc tcgggtttgt tacaacctcg
     2941 tagggttcat tcgcgcaggc agtgcgtgcg gcgaaaatcg agccgaacaa cagccatagc
     3001 aggaagaagg tacggccgat gattattcgg gtagccacaa ccagcttttt tgtgcccata
     3061 taaacgtgga acgcatgacc gccgttttcc acgcatgggg agggagattg agttgatgtc
     3121 ccggcgaagg ctgcgccagg ataggaggtt ttttcacggc ttcgggctat tccagggcct
     3181 caccttgcgt tgcttcacat aacaatcctt cacacggtcg ttgcgtgatg gacggctgga
     3241 cttttccgcc atccgtaacc aactcccaaa actgtctgtg gttcctatgc attctgtcca
     3301 gcatgtgggg aaagtcggcg taatagggac ggcgcagacc gtgatggtcg atgagcggac
     3361 cgacgggatc gaggtggagt ccatacaacg acaacaatcg gttcagagcg ggctccatca
     3421 ccgagagcca gtggttgata ttgttgtcaa cagacatccg gataatgccg acagcaagtg
     3481 cgagcatggg gtgcggaaaa cgccgtttga cagtggggct ccacttttcg atattgcgtc
     3541 cgttctccaa agcatcgatc agttcatctt tgcgccgatt gaatcttcgt atcacaacca
     3601 ggcgcgaaat ttccccggtt tgtttccgcg gtaaccgtgc tgtgttgaga agggcagggt
     3661 caaaacgcat gtgccgctcg gttgggaata gtttgttaag atgcagcgga tcgggcagta
     3721 tcatgcgtgc agtaccgaca aacactccgg atggcctgtg gcgcaaaaga atgtgggaag
     3781 agtggcggtc gtagttatcg ctttccattt cgtctggata gctggatgca tcaaacccgg
     3841 gagcgcgttg ctcaatacaa agaacccggt accgtaggtt gaatacctca cgtaacaatt
     3901 cgacggaatt gacgttggta atttcgaagt attcgtgaaa agctgcgacc acgtcgttca
     3961 tcaggatggg tatgagaatg tgattgaaat cgacagattt tgaatgtttc cctgcaatct
     4021 gccgttcgtt gcactttgac ggtcaagact cgccatcccg gcaccaaaac gatgcagatg
     4081 ggaaacgttt taagcgcaat gcgccgtcca cgactgattt attcatatat cgtctctgtc
     4141 aaaaggcgct catagctatt ggcgaacgag agagatggca gctcgagcaa tgtgaatgcc
     4201 agaatgcaaa tggctttctt gcgtctttgt tatcagaaag ccagttattc cgacctgcgg
     4261 acacttcttc tccccatccg gcccgtagca ttaaagcgtt tggaacttta aatgatccgg
     4321 gcttgtttca aatgttgaga aagagcgtat tttccacttc agggaagggt ttttcgcagc
     4381 cgagcttaac agggggatta aacgacaatg ggatgctgaa gaatagctgt aagatgttac
     4441 agctggaaca ttacaacctg ccgtgctctt gagaatggtt atgatacgaa cgttgtagtc
     4501 ttcaatttgc ggaaaaggtt tcgcgggcaa gaaacgtgac gcccggggaa tcaggacaac
     4561 tgaacgcaaa taggcgcact cgtggctgtg ccgttacgct tttggagcgg gaggtatcat
     4621 ggggttaatc aaagcagccg gacgtccaat acacgacggt tatgccgtgc tgctataatt
     4681 cagtcacagt cattgaacgt ttcatggcgc atgcgagcaa tgcggatgaa tcgtgtaggg
     4741 tggtgcccag aagaggactc gaacctccac agtgttgcca ccgctaggac ct
//
LOCUS       DASSHJ010000215         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_657797, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000215 DASSHJ010000000
VERSION     DASSHJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657797"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFI43_08420"
     CDS             <1..798
                     /locus_tag="VFI43_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062185.1"
                     /translation="VTNTVAVYDAVLKVDTPEGPGWHRYTHDGYGQKADGSPYDGAGV
                     GRLWPLLTGERGHYELASGNSAYPYIKTMEAFSSTCCLLPEQVWDSADIPAKHMWIGR
                     PTGSAMPLVWSHAEYIKLLRSVHDGIVFDLIPIVRKRYIDDQVGTDLEVWTFKQKLSD
                     FRPWRRLRIEVYGAALLHWTENNWETAHDIELPDEGLGVFAHEFEKGRFGHATELKFT
                     FYWHQPQRWEGRDFSLQVNAGEPPQAVPKPAEEKHADLKEVFEPALD"
     gene            830..1216
                     /locus_tag="VFI43_08425"
     CDS             830..1216
                     /locus_tag="VFI43_08425"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HET7062186.1"
                     /translation="MNNTDGQVPGHKRWVVADGYIPPDSTGSTRPLVSHDAICILNCA
                     NKDAHITVTVFFSNRDPAGPYSFNVSAMRTKHVRFNELSDPEPIPHGTDYASVIESDI
                     PVIVQHTRLDSRQAALALMTTIAYPA"
     gene            1227..1418
                     /locus_tag="VFI43_08430"
     CDS             1227..1418
                     /locus_tag="VFI43_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062187.1"
                     /translation="MKHPTAIIFSCDLAVLPVELPHCGEHIVGSDQAWVTLRADRQDQ
                     LKKAHDAPFLLCVVLPYPR"
     gene            1823..2884
                     /locus_tag="VFI43_08435"
     CDS             1823..2884
                     /locus_tag="VFI43_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7062188.1"
                     /translation="MADIRPAACRPSSAMRRLTRLTLLGGLTFASAICPAETLHAPLN
                     YFMHSFGPAARPTMYLGWVLAGISVAVGFIIAFLLIMAVRRQRPTAGEREIGREDEGM
                     QWIYMGTGISTCVLTALVVYSMMVLNAVAKPPEAPAVTVTVTAYDWWWKVEYDHPDPA
                     QHIVTANEIRIPVGQPVLVKLKSADVIHGFWVPTLAGKTQAIPGLVNQQWIQADIPGR
                     YTGQCTQYCGAAHARMGFEVIAEAAADFEKWREAQRQPASSGVAVSEDSGYKLFMDRC
                     AGCHAIKGTDAAGPHAPDLTHLNSRKRLAAGLLTNTPDNLMDWVTRAQQLKPGSRMPS
                     IYLSEAEGEALSAFLSKLD"
     gene            2903..4894
                     /gene="ctaD"
                     /locus_tag="VFI43_08440"
     CDS             2903..4894
                     /gene="ctaD"
                     /locus_tag="VFI43_08440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7062189.1"
                     /translation="MASADITPPRIDDDFPARLPETGRAPQGSDIERKLAATWESQPG
                     WRGWLSTVDHKAVGLRYIVTSFVFLVIGGIEALIIRIQLAQPDQSVLTPEQYNQLFTM
                     HGVTMIFLYSLPILSGFSNYLWPLLLGSRDMAFPRLNAFSYWIFLFAGIFMYASFPIG
                     QAPNGGWFNYVPFSGPMYNTGPNIDVFALGMVLLGISTSVGSANFVVTLFRMRAPGMT
                     INRVPILVWGTLTASVANLLVVPSVSLAFFLLWLDRQIGTYFFDVANGGKPLLWQHLF
                     WIFGHPWVYAVVLPAMGIVSDALPTFCRRPLVGYTAMALATMATMLIGMEVWIHHMFA
                     TGLPTVALSIFGAASMAITIPSSVAVFSWIATIWLGRPVFKTPFLFFAGFVMLFVIGG
                     LSGVMTAAVPLDWQLNETYFIVAHLHYVLIGINVFPVVGGIYYWFPKFTGRMMDEKMG
                     KWSFWVMFIGFNVGFFPMHWAGLLGMPRRIYTYSSRMGWDSVNMITTIGSFAFAVGVL
                     IFLVNVAVSLKRGARASENPWDAATLEWATSSPPPPYNFAVIPTVASRHPLWEDRLNE
                     GGARSNLTEGMLLADGRETVSTSPIDARPMAIFKMPEDSYTPFFLGLFISLVFVGLLL
                     HWWLFTAASATAGAIALATWLWPREELAQRATTQGATHG"
     gene            4887..5486
                     /locus_tag="VFI43_08445"
     CDS             4887..5486
                     /locus_tag="VFI43_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7062190.1"
                     /translation="MAEPVHLTYERLPVGSAGKISGGWWGVVTLIVTEACLFGYLLFC
                     FFYLNSQSEQEWPPDGLPGLLIPGINTVILLSSSVCVWVAERGIHQDRAAWTILGLVA
                     ALVLGTCFVAIQLIEWSNKPYGMTTDLYGSLYFTITGFHMAHVVIGLAVLLLLLVWAG
                     LGYFDSKRYAAMTIGGAYWHFVDVVWLFVFSSLYLFPYI"
     gene            5498..5971
                     /locus_tag="VFI43_08450"
     CDS             5498..5971
                     /locus_tag="VFI43_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062191.1"
                     /translation="MDFATNQDNPETNSSRRHRSPRHNSLLKVLVALFGAPVAWLAQM
                     SLSEPIAAYTCYPQQVPLSAPLWVELPAILAAISLACLAAGLFSAYVAWIFWRQSAVT
                     GMTINQGNDSQGAVIDGGQNRFLAMLGLMSSSLFVIAILFTCFAVFLVSPCSAWT"
BASE COUNT         1226 a         1747 c         1766 g         1481 t
ORIGIN      
        1 gtaacgaaca ccgtcgcagt atatgacgca gtgttgaaag tcgatacgcc ggagggtccg
       61 ggctggcacc gctataccca tgatggatac ggtcagaagg ctgacggctc tccctatgat
      121 ggcgcgggag tcggacggct atggccgctt ctgacgggag agcgcggaca ctacgaactc
      181 gcatcaggca actcagctta tccttacata aaaacaatgg aggcattttc cagcacctgc
      241 tgcctgttgc cggaacaggt ctgggacagt gccgatattc cggccaagca tatgtggatt
      301 gggcgtccca caggctcagc catgccgctg gtatggtccc acgccgaata catcaaactg
      361 cttcggtccg tccacgacgg aatcgtgttc gatctgatac caattgtgcg gaaacgttat
      421 atagatgacc aggtgggtac cgatcttgaa gtctggactt tcaagcagaa gctgagtgat
      481 tttcgtcctt ggcggcgcct tcgcatagag gtttacggag cggcgctgct gcattggaca
      541 gaaaataact gggagaccgc ccatgatata gagcttcccg acgagggctt gggtgttttc
      601 gcgcatgaat tcgagaaggg gcgattcggt catgcaaccg aactgaagtt cacattttac
      661 tggcatcaac cccaacggtg ggaaggccgt gatttcagcc tgcaagtcaa cgcaggggaa
      721 ccgccgcagg cggttccaaa acccgcggaa gaaaagcatg cggacctgaa agaagtattt
      781 gagcctgcgc tggactgagc agcgaatacg gaaaaaaaga ggtgacccta tgaacaacac
      841 tgatggacag gttcccggcc acaagcgctg ggttgtggcg gacgggtata ttcccccgga
      901 cagcaccggc agtacccggc ctctcgtcag ccatgacgca atctgtattc tcaattgcgc
      961 caacaaggat gcgcatatca cagtgaccgt ttttttcagc aatcgcgatc ccgccggtcc
     1021 ctactccttt aatgtcagcg cgatgcgtac aaagcatgtg cgcttcaacg agctttctga
     1081 tcccgagcct attccccacg gcactgacta cgccagcgtg atcgaatcgg acattcccgt
     1141 cattgtccag cacacccgcc tggattcgcg ccaagccgct cttgccctga tgaccacgat
     1201 agcctatccg gcatgacctg cagctgatga aacatcccac agccattatt ttcagctgcg
     1261 atctcgctgt gctgcccgtt gagttgccgc attgcgggga gcatatcgtt ggcagtgacc
     1321 aggcctgggt gacgttgcgc gcggaccggc aggaccagct taaaaaggca cacgatgctc
     1381 cgtttttgct atgtgtggtt ttaccgtatc cccgctgatg aagttggaac accggtccgt
     1441 gagaagggaa atctcatcta ttcgtttttt aattcggatg gacgagagga gggataggga
     1501 atcactgctt cggcgcgcgg tttgcccctt tactatactg agtggactcc tcttccaatc
     1561 cacacgattc catgcatgac gattcctatg caccgcgttc gcaatcaaga cgaggctcca
     1621 ggcgcctcaa acgcgtctcg atgcgtacag gctctacagc tgctcggatt ggacgagcag
     1681 cactatatct gtaccggcga tacccctcga tgtttccctc aacctcgcgc ctcagtcggc
     1741 tattgctctg gacattcagt tcaaacaacg gcgggccgcg ctccaggtca gcgaagcatg
     1801 aaccctcaca ggccgaaaca agatggcaga cattcgacca gcggcctgtc gcccctctag
     1861 tgcgatgcgc cggctcacac ggctgacact tctgggcggg ctgactttcg cctcggccat
     1921 atgcccggcc gagaccctcc atgcgccctt aaattatttc atgcacagtt ttgggccggc
     1981 agcccgcccc actatgtatc ttggctgggt gcttgcagga atatcagtcg ccgtcggctt
     2041 cattatcgca tttttgttga tcatggcggt tcgccgccag cgacctacag ccggggagcg
     2101 tgagattggg cgggaagacg aagggatgca gtggatctat atggggacgg gaatctccac
     2161 gtgcgtgctt acggcactgg tcgtttattc catgatggtg ttgaatgcgg tcgcgaaacc
     2221 gccggaagct ccggcagtta ccgtaacggt gacggcttac gattggtggt ggaaagtcga
     2281 gtatgaccac cctgatcccg cccagcatat cgtcaccgcc aatgaaattc gcatccctgt
     2341 gggacaaccg gtactggtca agctgaaaag cgccgatgtc atccatggtt tctgggtgcc
     2401 gacgctcgca gggaaaaccc aggcaattcc gggtctcgta aatcagcagt ggatccaggc
     2461 agacattccg gggcgttaca ccgggcaatg cacccagtat tgcggcgcgg cccacgcacg
     2521 catggggttt gaggtcatcg cggaagcggc ggccgatttc gagaagtggc gtgaagccca
     2581 gcgtcagccg gcttcatcag gtgtagcggt gagcgaagac tctggctata aattgtttat
     2641 ggatcggtgc gcgggttgcc acgcgatcaa gggaacggat gcggcgggac ctcatgcgcc
     2701 ggacctcacc cacttgaatt cccgaaagcg gctggcggca ggtttgctga ccaatacgcc
     2761 ggacaacttg atggattggg tcacccgcgc ccagcagctc aagcccggct cgcggatgcc
     2821 cagcatatat ctgtccgaag ccgagggcga ggcgctctcg gcatttctat cgaaactgga
     2881 ttagaacgtt actggaatag cgatggcaag tgccgatatt accccgccca gaatcgatga
     2941 tgattttccc gcccgattgc cagagacggg ccgtgcgccg cagggatcgg atatcgagcg
     3001 caagctggcc gcgacgtggg aatctcagcc cggctggcgc ggatggctct cgaccgtgga
     3061 tcacaaagcc gtcgggctcc gctatatcgt tacgtccttc gttttcctgg tcatcggggg
     3121 gatcgaggcg ctcatcatac gaattcagct tgctcagccc gatcagtcgg tcctaacccc
     3181 cgagcaatac aatcagcttt tcaccatgca cggcgtgacg atgattttcc tttactcact
     3241 gccgatattg tccggatttt cgaattacct ctggcccttg ctgctgggct cgcgcgacat
     3301 ggcctttccc cgcctcaacg cgttttccta ctggatattc ctgtttgccg gcattttcat
     3361 gtacgccagc tttcccatcg gccaagcgcc caacggagga tggttcaatt atgtcccttt
     3421 ctctgggccg atgtataaca caggaccgaa catcgatgtc tttgcgcttg gcatggtcct
     3481 gctgggcata tccacgtcag tgggatcggc aaattttgtc gtgacgctgt tcaggatgcg
     3541 ggcgccggga atgacgataa atcgggtgcc gatcctggtg tggggaacgc ttaccgcttc
     3601 cgtcgccaat cttctcgtgg ttccttcggt cagcctggct ttcttcctcc tgtggctcga
     3661 tcggcagatc gggacgtatt tctttgacgt agccaatggc ggtaagccac tgctttggca
     3721 gcatctgttc tggatcttcg gccatccctg ggtctatgcc gttgtgctcc ccgcgatggg
     3781 aatcgtctcg gatgccctgc cgaccttctg ccgccgcccc ttggtcggtt acaccgccat
     3841 ggccctcgcg accatggcta ccatgctgat cggcatggag gtctggatac atcacatgtt
     3901 tgcgacggga ttgccaacgg ttgcgctgtc gatatttggc gccgccagca tggcgataac
     3961 gatcccgagt tctgtcgcag tattctcctg gattgcaacc atctggctcg gacggcccgt
     4021 ttttaaaacg ccgtttttat tttttgccgg cttcgtgatg cttttcgtaa tcggcggctt
     4081 atcgggagtg atgaccgccg cggtgccact ggattggcag cttaacgaga catattttat
     4141 cgttgctcac ctgcactatg ttctgatcgg catcaatgtg tttccggtcg tcgggggcat
     4201 ctactactgg tttccgaaat ttacggggcg catgatggac gaaaagatgg ggaaatggag
     4261 cttttgggtg atgttcatcg gctttaacgt cgggtttttc cccatgcact gggcgggcct
     4321 gctcggaatg ccccgccgca tctacaccta ttcctccagg atggggtggg acagcgtcaa
     4381 tatgatcacc acgatcggct ctttcgcatt cgcagtgggc gtcctgatct ttctggtgaa
     4441 cgtcgctgtc agcctcaagc gtggcgcccg agcgagcgag aatccgtggg atgccgcaac
     4501 gctggagtgg gcgacatcat cgcctccccc accctacaac tttgccgtta ttcccaccgt
     4561 ggcaagccga catcctttat gggaggatcg actgaacgag ggcggagcgc gctcgaattt
     4621 gactgaaggc atgctgcttg ccgatggacg ggagacggtc agtacctctc cgatcgacgc
     4681 gagacctatg gccattttca agatgccgga agattcctac acgccgtttt ttcttggcct
     4741 gttcatttcc ctggtttttg tcggcctgct tttgcattgg tggctgttca cagccgcaag
     4801 tgctacggca ggagccatcg cgctggcaac atggctgtgg ccaagggaag agcttgcaca
     4861 gcgggcaaca acgcagggag ctacccatgg ctgaacctgt tcatctgaca tacgagcggc
     4921 tgcctgtcgg aagcgccggt aaaatttccg gcggctggtg gggcgtggtg acattgatcg
     4981 tcaccgaagc gtgcctgttt ggctatttgt tattctgctt tttctacctg aattctcaat
     5041 ccgagcagga atggccgccc gatggtctgc ccggcctgct catcccaggc ataaataccg
     5101 tcattctgct gtcgagtagc gtctgtgtct gggtcgctga acgcggcatc caccaggacc
     5161 gggcggcctg gacgatcctc ggtctcgtcg ctgcgctcgt gctcggcacc tgtttcgtcg
     5221 caatccagct catcgagtgg agcaacaaac cttatggcat gacgaccgac ttgtatggat
     5281 cgctctactt tacgataacg ggtttccaca tggcccatgt cgtcattgga ttggccgtac
     5341 tgttactcct gctcgtatgg gcgggattgg gttatttcga cagcaaacgt tatgcggcaa
     5401 tgacgattgg cggagcctac tggcacttcg tggatgttgt ctggctgttc gtcttttcca
     5461 gcctttacct gttcccttat atttaggaag ttgcggaatg gactttgcca cgaaccagga
     5521 caacccggaa acgaatagct cccgacgaca tcgctcgcct cgccacaact ccttgctcaa
     5581 ggtgctcgtc gcgctatttg gcgctccggt tgcgtggctc gctcaaatgt ccctaagtga
     5641 accgattgca gcttatacgt gctaccccca gcaggtgccc ctctcggccc cgctttgggt
     5701 tgaactacct gccattctcg ccgcgatcag cctggcctgc ctcgcggctg ggttattctc
     5761 ggcatatgtt gcctggatct tctggcggca atcagctgtc acgggcatga cgatcaatca
     5821 aggaaatgat agtcagggcg ctgtgatcga cggcgggcag aaccgttttt tggcaatgct
     5881 cggactgatg tcgagcagcc tgttcgtcat tgccattctg tttacctgtt tcgcagtttt
     5941 tcttgtatcg ccttgcagcg cctggacttg atgagattga aagcggcctg gttcgtaggg
     6001 tattgggagt gctgctatgg attcgattgg cggaggatgg aaagctttac ataattcatg
     6061 ggctgtcctg ctcatactcg cagcaaacgc tcaggctgcg caggaaaacc ttgtagcaga
     6121 taaccttgtt gcccgtggcg catatcttgc gaaagccgca gattgcgccg ggtgtcatac
     6181 ggccggtcga gggcatccgt catatgccgg gggcttggcc
//
LOCUS       DASSHJ010000216        10354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1122272,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000216 DASSHJ010000000
VERSION     DASSHJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122272"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFI43_08455"
     CDS             <1..1182
                     /locus_tag="VFI43_08455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7062192.1"
                     /translation="DLAKQIAVTDEEAEKYFDEHRAEFEQAEERRASHILINAPESAP
                     EAEKSAARTKAQQLLTQIRQAPQNFEKLAGEHSQDSGTAQKGGDLGFFSRNMMVKSFE
                     DAVFQMKLNEISDVVESNFGFHIIKLTGIKAGKPVSFGEVKGQVEEEIRKQKARKLFD
                     EMSEGFSNTVYEQSDSLKPAAEKFHLAVQKSGWIGKNSGFPPYFSNAKLLQAVFSDDA
                     IKNKHNTEAIEVAPNTLVSARVLEHRPASTPSLDELKDKIKALVARQEATSAAVGEGK
                     DKLAQLRENKNPAVSWSSPQQVSRREPQGLDMGTLRAVFAADTSRLPAFAGLPNPEGG
                     FTLVRVTKVIEPALPDEATRKLFGRQLHQVLTEEELASYLGGIRKRYDVTVKPENLDK
                     K"
     gene            complement(1235..2023)
                     /gene="fabI"
                     /locus_tag="VFI43_08460"
     CDS             complement(1235..2023)
                     /gene="fabI"
                     /locus_tag="VFI43_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HET7062193.1"
                     /translation="MGFLAGKRILITGLLSNRSIAYGIAKAMHREGAELAFTYQGEGI
                     RERVAKTAEEFGSTLLFPCDVSSDAEIERCFDDLGKHWDGLDGIVHSVAYAPREALSG
                     DFLESINREAFRVAHDISSYSFAALAKAGVPMMQGRNGSLLTLTYLGAVRVMPNYNVM
                     GLAKASLEANVRFMASSLGPQGIRVNAISAGPIKTLAAAGIANFGKLLGYTEKVAPLR
                     RNVSILEVGNTAAFLCSDLASGITGEITYVDAGFNTVAFDIIEP"
     gene            2222..4450
                     /locus_tag="VFI43_08465"
     CDS             2222..4450
                     /locus_tag="VFI43_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7062194.1"
                     /translation="MTRLFQYLLMLPVVLLNACSGNPWNNPYPAAEAGKNILYSAFSE
                     RPKHLDPVQSYSSNEIQFTAQIYEPPLQYHYFKRPYELIPLAAAKLPVTQYFDKRGNR
                     LPDNVDSAQIAYTIYEITIKPGIRYQPHPAFAKDEQGNFLYHRLDAADLAKIFKLGDF
                     PRTATRELHAEDYVFQIKRLAHPRLHSPIFGLMSDYIVGLRDYSTLLQTAAVGLVQES
                     GDAYLDLNRYALEGAEVVDQYTYRVKIKGKYPQFMYWLAMPFFAPIPWEAERFYSQKG
                     LASKNISLDWYPLGTGPYMLTENNPNRIMVLEKNPNFHGETYPAEGMPGDAKAGFLDD
                     AGKPLPFIDKVVFSRERESIPRWNKFLQGYYDASSIGSDSFDQAVQLTGQGEATVTEA
                     MQEQGIRLETTVAASTSYMGFNMLDPVVGGVGKRGREAARKLRQAISIAVDYEEYVSI
                     FANGRGVPAQGPIPPGIFGHREGKEGINPVIYDWVNDHAQRKPIDAAKALLAEAGYPG
                     GVDAKTGALLVLYFDVTSRGAEDKPSLDWMRKQFQKLNIQLVVRSTDYNRFQDKIRKG
                     NAQIFEWGWNADYPDPENMLFLLHGPQRKAGHEGENASNYSNPEYDALFDQMKNMENG
                     PERQRIIDRMVNILRHDAPWLFGYHPKDYGLYHAWYDNVKPNRMSNNSMKYFRIDADL
                     REQKRRDWNSPVLWPVAIGLILLAAILIPAIHAYRRKERGAALESRNLNNGAAAQGIA
                     GS"
     gene            4529..5506
                     /locus_tag="VFI43_08470"
     CDS             4529..5506
                     /locus_tag="VFI43_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7062195.1"
                     /translation="MLNYILRRMLYAIPILIGVNLITFTLFFVVNTPDDMARMQLGVK
                     RVTPAAIAKWKQERGYDKPLMFNASETGIAKLTETIFFEKSVSMFAFRFGQSDDGRDI
                     AREIRTRMMPSLAIALPVFALGLCVYVAFALVMVFFRATYVDFWGVVICVALMSISSL
                     FYIIGGQFLISKLWHLVPISGYGEGLDTAKFLVLPVVVGVISGMGANTRWYRTIFLEE
                     MGKDYVRTARAKGLPERLVLFRHVLKNAMIPILTGVVVVIPLLFLGSLLAESFFGIPG
                     LGSYTIDAISSQDFAIVRAMVFLGSLLYIVGLILTDMSYTLVDPRIRLE"
     gene            5521..6999
                     /locus_tag="VFI43_08475"
     CDS             5521..6999
                     /locus_tag="VFI43_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7062196.1"
                     /translation="MPFTPVIFWTDALVYLLALVIAVFVWQVRRNEHLLVPWRRVGQS
                     ASGMAALTALLFFVVVGLLDTVHVRFPVNDGAGVEKTYSAEVLSLLDVIATPLRTRVE
                     KTYSAPFAAHLYAKENIELPDGAQARDFPRLKFGGVHLADPKAQRSEDIISRALKGSG
                     IGLLVWCMIVAALTMGLAHRAGRPFGSTWAAMWRGTTGIRWNAILVTLLLMLLLWGAA
                     LALCSYYHILGTDKVGQDVFYLSLKSIRTGLVIGTLTTLMMLPFALFLGITAGYFQGW
                     VDDVIQYLYTTLSSIPSVLLIAAAVLMMQVYMETHPELFETIAARADLRLLFLCIILG
                     VTSWTGLCRLLRGETLKLREMEYIQAAHAFGVGHWRIISRHILPNVMHIVLITTVMDF
                     SGLVLAEAVLSYVGVGVDPSMISFGTMINAARLEMAREPMVWWSLLSAFSFMFTLVLS
                     ANLFADAVQNAFDPRIMIISPKAAIKDSASRGGEHSPAISHQ"
     gene            7015..9159
                     /locus_tag="VFI43_08480"
     CDS             7015..9159
                     /locus_tag="VFI43_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7062197.1"
                     /translation="MHPLLQVDNLTTVIHTATGLAWPVDGLSLKIEKGETFALLGESG
                     CGKSITALSIMRLLPEAGAIAAGSVCLDGQDLLLLPETSMRDVRGRRISMIFQEPMLS
                     LNPVMTVAEQIGEVLQRHFGLGGTVARERILEILNQVGIPDAARRMDEYPFQFSGGMK
                     QRMMIAMALAGEPELLIADEPTTALDVTIQAQVLDLMRSLQQRNGMSILLITHDLAVA
                     AETADRIGIMYAGEIVETAPRDVFFRGPAHPYSRKLLASVPGRDRRGAGTRLSAIPGN
                     VPSPSHKFSGCRFADRCDRAWALCLEAAPGWSAVGDGQQARCHLLEHDSSSFALLPSR
                     NEKGEMQARNSGTAAEAGSKVTVLHRPVTAPEPDLLAVTDLKVHFPIYKGLIKKAVGA
                     VKAVDGVSLSLRRGRTLALVGESGCGKTTVGKGILQLIPVSGGSVKYNGIELVGLESK
                     RLRELRAEFQLVFQDPYSSLNPRMRIMEVIREGMNALNVKNHGKEHSGNRQSDGSGGE
                     VDRLLESVGLRPEAKWRYPHEFSGGQRQRIAIARALAVRPKLIVCDEPTSALDVSVQA
                     QILNLLGDLQDSLELAYLFITHNISVVEYIADEVAVMYLGRIVESGSVDEVLNSPKHP
                     YTQALLSAVPVIEPESKRHIIRLQGDLPSPSNPPSGCHFHPRCPHAMPICREVYPATS
                     RFSPTHQTHCHLYPQDKVAKGNHAQEYVYDGR"
     gene            9146..>10354
                     /locus_tag="VFI43_08485"
     CDS             9146..>10354
                     /locus_tag="VFI43_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET7062198.1"
                     /translation="MTVGKEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVK
                     ARKASRHATSDWMEIEKQRGISVASSVMQMEYEGCVINLLDTPGHQDFSEDTYRVLTA
                     VDAALMVIDAANGVEAQTLRLLDVCRARNTPILTFINKMDREVREPLDLIDEIERTLR
                     MSAVPFTWPMGMGRRFRGVFDLQRDRMRVFQPGEDKLNDDTEVIEGLDNPVIDARFPD
                     LAEATRQEIELIRGASSAFDRAEFLSGKQTPVFFGSAINNFGIREVLDALVELAPPPR
                     SRSAFQRQVQPGETRFSGVVFKIQANMNPAHRDRIAFVRVCSGRFERGMNLRVARNGK
                     DLRTNSVVSFLSQRRELLETAYPGDIIGIPNHGTLQLGDTLTEGECLQFTGLPFFAPE
                     IFQTVEIADPM"
BASE COUNT         2353 a         2752 c         2910 g         2339 t
ORIGIN      
        1 gatctggcca aacagatagc cgtaaccgac gaggaggctg aaaaatattt cgacgagcat
       61 cgggccgagt tcgaacaggc ggaggagcgg cgggcaagcc atattctgat aaatgctcct
      121 gaaagcgcgc ccgaggcgga gaagtcggct gcacggacaa aggcgcagca gttgcttacg
      181 caaataagac aagcgccgca gaattttgaa aaacttgccg gggagcattc acaggattcg
      241 ggcaccgcgc agaaaggcgg cgatctcgga tttttttccc gtaatatgat ggtcaagtcg
      301 tttgaggatg ctgttttcca aatgaaattg aacgagatca gtgatgtggt cgagagcaat
      361 ttcggatttc acattatcaa gctcacgggg ataaaggcgg gaaaaccggt gagcttcggc
      421 gaagtgaaag ggcaggtgga ggaggagatc aggaaacaaa aagcgcggaa gctctttgac
      481 gagatgtcgg aaggtttcag caatacggtt tacgaacaga gcgacagcct caagccggct
      541 gctgaaaagt ttcacctggc tgttcaaaag agcggctgga taggaaaaaa cagcggtttt
      601 ccgccttact tcagtaatgc aaaattgctg caggccgtat tttcggatga tgcgatcaag
      661 aataagcata atacggaagc gatcgaggtt gctccgaaca cgcttgtctc agcaagggta
      721 ttggaacatc ggccagcttc gacaccctct ctcgacgaac tgaaggacaa gatcaaggcg
      781 ctggttgcac gacaggaagc aaccagcgcc gcagtgggcg agggtaagga caagctcgcg
      841 cagcttcgcg aaaacaagaa ccccgccgtt tcctggagct ctccccagca ggtttccagg
      901 cgcgagccgc aaggcctcga tatgggaacg cttcgagccg tctttgccgc ggatacgagc
      961 cggttaccag cttttgcagg gctaccgaac ccggaaggag ggtttaccct cgttcgagtg
     1021 accaaggtta tcgaacctgc tctcccggat gaggctaccc gcaagctttt tggaaggcaa
     1081 ctgcaccagg tgctcaccga ggaagaactt gcatcttatc tcggaggcat caggaagaga
     1141 tatgacgtaa ccgtgaaacc ggaaaacctg gacaagaaat gaagtgacgc tatggcagca
     1201 gcccgaacag cagtaattag ataaataagt cggttcaggg ctcgatgatg tcgaatgcaa
     1261 cagtattgaa tcctgcatcc acataagtta tttcaccggt gatcccgctt gcaagatcgc
     1321 tgcatagaaa agcggcggta tttcctactt ccaagatcga cacattccgg cgcagtgggg
     1381 caaccttctc ggtatagccg agcaattttc cgaagttggc gattccagca gcggccagag
     1441 ttttaatggg acctgcggaa atcgcgttca cccgaatccc ctggggaccg aggctcgatg
     1501 ccatgaatcg tacgtttgcc tcaagactcg ctttagccag acccattaca ttgtaattgg
     1561 gcatgactcg aaccgcgccg agatatgtaa gagtcaggag cgacccattg cggccttgca
     1621 tcatcgggac gcccgctttt gccaaagcag caaagctgta ggaactgata tcgtgcgcaa
     1681 cgcgaaatgc ctcacgattg atgctctcca ggaaatcccc gctcaacgcc tcacgcggcg
     1741 cataggcaac cgaatggaca atcccgtcga gcccgtccca atgctttccc aaatcatcga
     1801 aacagcgctc gatctcggcg tcactcgaaa cgtcgcatgg gaacaggagt gtacttccga
     1861 attcctccgc tgtttttgca acgcgctccc gaataccttc tccctgatag gtaaaggcca
     1921 gttcagcgcc ttcccggtgc atcgcctttg caatgccgta agcgatggaa cggttgctga
     1981 gcaaaccggt gataaggatg cgcttgccgg caagaaaacc catgcggact cctttagaag
     2041 taaactcgtg attatagcta caagtaaggg gctaataaaa tcgcaaacat tcgatagcgg
     2101 agggtggaac ctcgatacga accgttactc caaatgcttc cagccctcca atacactttg
     2161 cggctcaaaa catccggtct ctgaagcggt tcactgatat cttgttcttt cctcttcttt
     2221 aatgacgcgt ctgtttcaat atctattgat gttgccggtg gttctgctga acgcctgtag
     2281 cggcaatccg tggaacaatc cctatcccgc tgccgaagcc gggaaaaata ttctctatag
     2341 cgcattttcg gagcgtccga agcacctgga ccccgtgcaa tcatacagtt cgaacgagat
     2401 tcagttcacg gcacagatat acgagcctcc attgcaatat cactatttca agcggccata
     2461 cgagttgatt ccgcttgccg cggcaaaact gccggtcacc caatattttg acaaaagggg
     2521 aaaccgcttg ccggacaacg ttgactccgc ccagattgcc tacacgatat acgagatcac
     2581 cataaaaccc ggcatccgct atcagcctca tccagccttt gcaaaagacg agcagggcaa
     2641 ctttctctat catcgtcttg atgcagccga cctggcaaag atcttcaagc tgggcgattt
     2701 tccccgcacc gcgacccggg agctgcatgc ggaagattac gttttccaga ttaagcggct
     2761 cgcccatcca cggctgcact cgccgatttt cggtttgatg tcggactata tcgtgggcct
     2821 ccgggactat tcaacacttt tgcagaccgc tgcggtcggt ctggtacagg aaagtggcga
     2881 tgcctacctg gatttgaaca ggtatgcgct ggagggtgcg gaggtcgttg atcagtatac
     2941 ctatcgcgtc aaaatcaaag gcaagtaccc ccagttcatg tattggctgg ctatgccatt
     3001 ttttgcaccg attccctggg aggctgagcg gttttacagc cagaagggac tggccagcaa
     3061 aaacatcagc ctcgactggt atcccttggg tacgggtccc tacatgctga cggaaaacaa
     3121 ccccaatcgg ataatggttc ttgagaagaa tccgaatttc catggagaga cttacccggc
     3181 cgaaggaatg ccaggtgacg cgaaggccgg gtttctcgat gatgccggca agcctttgcc
     3241 gttcattgat aaggtcgtat tcagccggga gagagaaagt attccgcgct ggaacaagtt
     3301 tcttcagggt tactacgatg cctccagcat tggttcagac agcttcgatc aggcagtaca
     3361 actcaccggt caaggtgaag ccacagtaac ggaagcgatg caggaacagg gcatccgcct
     3421 ggaaaccaca gtagcggcgt ctaccagcta catgggtttc aacatgttgg atcctgtggt
     3481 gggcggcgtg ggcaagcggg gacgagaagc tgcccggaag ctacgccagg caatttccat
     3541 agccgtggat tacgaagaat atgtctcgat tttcgccaat ggaagaggag tgcctgctca
     3601 gggccccata ccgcctggaa ttttcgggca ccgagagggc aaggaaggaa taaaccctgt
     3661 catttacgac tgggtaaacg atcatgcaca gcgaaaaccg atagatgcgg ccaaagcctt
     3721 gctggcggag gccggatacc cgggcggcgt tgacgcaaag acgggagcgc tcctggtgct
     3781 gtatttcgac gtgacctcac ggggggccga ggacaaaccc agcctcgact ggatgcgtaa
     3841 acaatttcag aagctcaaca ttcagctggt cgtccggagc actgactata accggttcca
     3901 ggacaagata cgcaagggga acgcccagat tttcgagtgg gggtggaatg cggactaccc
     3961 cgatcccgag aacatgcttt ttttattgca tggcccacag cgcaaggccg gtcacgaagg
     4021 tgaaaacgcc agcaactatt ccaacccgga atatgacgcg ttattcgacc agatgaaaaa
     4081 tatggaaaat ggcccggaac gccagcgcat cattgaccgg atggtaaata tcctccgcca
     4141 tgatgcgcca tggttgtttg gataccaccc caaggattac ggcctctatc acgcatggta
     4201 tgacaacgtc aaaccaaaca ggatgtccaa taacagcatg aaatatttcc ggatcgatgc
     4261 cgacttgcgg gagcagaagc gccgcgactg gaacagcccc gttctatggc ccgtggcgat
     4321 cggcttgatc ctgttggccg ccatactgat ccctgctatc catgcctacc gccgcaagga
     4381 aaggggagcc gctctggaaa gccgtaactt gaacaacggc gcagcggctc aagggatagc
     4441 cggttcgtaa tgcctcgtga cgacactgct attctgcctc tcacccatct ggggaatttt
     4501 gcccgcctgt taccgatcgg ttgcttgaat gcttaactat atcctccggc ggatgctata
     4561 tgccatccct atcctgatcg gagtgaatct gattacgttt acgctgtttt ttgtcgtaaa
     4621 cacgcctgat gacatggcgc gcatgcagct gggcgtcaag cgcgttaccc ctgccgcgat
     4681 tgcaaaatgg aagcaggagc gcggctacga caagccgctc atgtttaacg cctccgaaac
     4741 cggcattgcc aaacttaccg aaaccatctt ctttgagaaa tctgtctcca tgtttgcatt
     4801 ccgcttcggg caatccgacg atgggcgcga tattgcgcgc gagatcagga cgcgtatgat
     4861 gccgagcctg gcgattgcgc ttccggtatt tgcgctgggg ctatgcgttt atgttgcgtt
     4921 cgcgctggtt atggtttttt ttcgcgcaac ctacgtggat ttctggggcg tcgtgatctg
     4981 tgtcgccctg atgtcgatct ccagcctgtt ttacatcatc ggtggtcagt ttcttattag
     5041 caagctgtgg cacctggtgc cgatctcggg atatggagaa gggctggaca ccgccaaatt
     5101 ccttgtcttg ccggtcgttg tcggggtaat cagcggcatg ggagccaata cgcgctggta
     5161 ccgtactatt tttcttgagg agatggggaa agattacgtg cggacggcac gcgccaaagg
     5221 cttgcccgaa aggctcgtgc tattcaggca tgtgctcaag aatgcgatga ttcccatact
     5281 taccggagtg gtcgtcgtca ttcctttgtt atttttgggc agtcttctgg cggaatcctt
     5341 tttcggcatc ccgggattgg gcagttatac gatcgacgcg atcagttccc aggattttgc
     5401 tatcgtgcgt gcgatggttt tcctggggtc tctgctctac atcgtgggcc tgatactcac
     5461 cgacatgtcc tatacgctgg ttgatcccag gatcaggctc gagtaatcgt caaaaatatt
     5521 atgccgttca cacctgtaat cttctggaca gacgcgctcg tctatttgct tgctctcgtc
     5581 attgccgtct tcgtatggca agtgcggcgc aatgaacatc ttctggtgcc atggcgccgc
     5641 gtgggccaga gcgcaagcgg catggctgca ttgaccgcgc tgcttttttt tgtggtggtc
     5701 gggctcctgg ataccgtaca cgtgcgtttc ccagtcaacg acggggctgg ggttgaaaaa
     5761 acctatagcg ctgaagtgct gagcctgctc gatgtgatcg cgacgccgtt gcgaacccgg
     5821 gtcgaaaaaa cctattccgc gccttttgcc gcgcacctgt atgccaaaga gaacatagag
     5881 ctaccggatg gcgcccaggc ccgggacttt cctcgcctca agtttggcgg agtccatctt
     5941 gccgatccga aagcacagcg gtcagaagac attatctcgc gggcgctcaa ggggagcggg
     6001 atcggccttc tcgtctggtg catgattgtg gctgcgctga cgatggggtt ggcgcatcgc
     6061 gccggacggc catttggaag cacgtgggcg gccatgtggc gcggtacaac gggtatacgg
     6121 tggaacgcta tacttgttac gcttttatta atgctgctac tctggggggc tgcgctcgcg
     6181 ctatgcagct attaccacat tcttggcacg gataaggtag gtcaggacgt attttacctg
     6241 tcgctgaaaa gcatacggac cgggttggta atcggaacgc tcaccacgct gatgatgctt
     6301 ccgttcgcgc tatttctagg gatcactgcc ggatacttcc agggctgggt ggacgacgtg
     6361 atccagtatc tttatacgac gctgagttca attccaagcg ttctgcttat tgcggcggct
     6421 gtgctgatga tgcaggtata tatggaaacg catcccgagc ttttcgagac cattgctgcg
     6481 cgcgcggatc tgcgcctgct gtttctttgc atcatactcg gcgtcaccag ttggaccggc
     6541 ctgtgccgcc tgctgcgcgg cgagaccctg aagttgcggg agatggagta tatacaggcc
     6601 gctcatgcgt ttggcgtggg gcactggcgc attatttccc gccacatttt gcccaatgtc
     6661 atgcatatcg tgttgatcac gacggtcatg gacttcagcg ggcttgtcct ggctgaagcg
     6721 gtgctttcct atgtgggggt aggcgtcgac ccctcgatga tcagtttcgg aacgatgatc
     6781 aacgcagctc gcctggaaat ggctcgtgag cctatggtct ggtggtcttt gctctccgca
     6841 ttcagtttca tgtttaccct ggtgctgagc gccaacctgt ttgcggatgc cgtacaaaat
     6901 gctttcgatc cgaggattat gataatttcg ccgaaagccg cgataaaaga cagtgcaagc
     6961 cgggggggtg aacattctcc tgccatatct caccagtagt gtctcgctta tcctatgcat
     7021 cccttactgc aagtcgacaa cctcacgacc gttattcata ccgctaccgg gctggcatgg
     7081 cccgttgacg ggctctccct gaaaatcgag aagggtgaaa cctttgcgct gctcggggaa
     7141 tccggctgcg gcaaatccat cactgcgctg tccatcatgc ggcttttgcc ggaggcaggt
     7201 gcaattgctg cgggttccgt ctgtcttgat ggtcaggatc tgcttctgct ccccgaaaca
     7261 tccatgcgcg acgttcgcgg acgtcgtatc agcatgatct ttcaggaacc catgctgagc
     7321 ctgaacccgg tgatgaccgt cgcggagcag atcggggaag tattgcagag acattttggt
     7381 ttagggggaa cggttgcgcg agagcgtatc cttgaaatat tgaatcaggt gggaataccg
     7441 gatgcggcgc gtcgcatgga tgagtatccc ttccagtttt caggggggat gaagcagcgg
     7501 atgatgattg caatggcgct ggcaggagag cccgaattgc tgattgctga tgaacctacc
     7561 accgcgctgg atgtcaccat ccaggcccag gtactggatc tgatgcgcag cctgcagcag
     7621 cgcaacggca tgtccatcct gctcatcacg catgatctcg ctgtcgcggc ggaaacggcc
     7681 gaccggatag gaatcatgta cgcaggcgaa attgtagaga ccgctccccg tgacgttttt
     7741 ttccgcggcc ctgctcaccc ctactcaagg aagctgcttg cgtcagtgcc cggaagggat
     7801 cggcgcggcg ccggaacgcg gctgtcggca attcctggaa acgtcccttc gccgtcgcat
     7861 aaattctcgg gctgccgatt cgctgaccgc tgcgaccgcg cctgggccct gtgcctcgaa
     7921 gctgccccgg gctggtcagc tgtaggcgat ggacagcaag cccgatgcca cctgcttgaa
     7981 catgattcaa gcagtttcgc tctcttgcct tcgagaaatg aaaagggtga gatgcaggca
     8041 cggaattctg gaactgctgc tgaggcaggg tcgaaggtaa cggttctcca tcggccggtc
     8101 acagcacccg agccagattt gttggccgtc accgatctta aagtccactt cccgatatac
     8161 aagggcctga tcaagaaagc agtgggtgcg gtcaaggcag tcgatggcgt ttcgctatcc
     8221 cttcgccgcg gccgaacatt ggcgcttgta ggagagtcgg gttgcggcaa gacaaccgta
     8281 ggcaagggga ttctgcaact cattccggtc agtggaggca gcgtcaagta caacggaatc
     8341 gagttggtcg gactggaaag caaacgtttg cgcgagctgc gcgcggaatt tcagctcgtc
     8401 ttccaggatc catattcatc cctcaatccc cggatgagaa tcatggaagt tatccgggaa
     8461 ggcatgaatg cgttaaacgt taaaaaccat ggcaaagagc attccggcaa caggcaatcg
     8521 gacgggtcgg ggggcgaggt ggatcgcttg ttggagagcg tcggactgcg tccggaagcc
     8581 aagtggcgtt accctcacga attttcaggg ggacagcggc aacgcattgc cattgcccgc
     8641 gccttggcgg tacgcccaaa gctgatcgtc tgcgatgagc ccaccagcgc gctcgacgta
     8701 tcggtgcagg cccagatcct gaatttgcta ggggatttgc aggacagtct ggagctggcc
     8761 tatctcttca ttacgcacaa catctcggta gtggaataca ttgcggatga ggtagcggtc
     8821 atgtatctcg gaagaatcgt tgaatccgga agcgtggatg aagtgttgaa cagtcccaag
     8881 catccttata cccaggcatt gctgtccgcg gtcccggtca tcgaacccga atcgaaaagg
     8941 cacatcatcc ggcttcaagg tgatcttcca tcccccagta acccgccgtc cgggtgtcac
     9001 tttcatcccc gctgcccgca tgcaatgcca atttgccgtg aagtctatcc ggcaaccagc
     9061 aggttcagcc caacacatca gacgcactgc cacctctatc cccaggacaa agtcgctaag
     9121 ggcaatcacg cacaggagta tgtttatgac ggtcggtaag gaggtggcga gacgacgcac
     9181 atttgccatc atatctcacc cggatgcggg taaaaccacg cttacggaga aacttctgtt
     9241 gtttgccggt gcgattcaca tcgcgggtag cgtcaaggca agaaaggcga gccgccatgc
     9301 gacctcggac tggatggaaa tcgagaagca gcggggcatt tccgttgcca gctcggtaat
     9361 gcagatggaa tatgagggtt gtgttatcaa cctgctggat acgcccggcc accaggactt
     9421 ttcggaagat acctatcgcg tcctgaccgc ggtggatgct gcgttgatgg taatcgatgc
     9481 tgccaatggc gtcgaggcgc agaccctgcg cctgctcgac gtctgccgcg cgcgcaacac
     9541 gcccattctg accttcatca ataaaatgga cagggaggtg cgtgaacccc ttgatcttat
     9601 cgatgagatc gaacgaacct tgcgcatgag cgccgttcct tttacctggc ccatgggcat
     9661 gggccggagg ttcaggggcg tatttgacct tcagcgggac cgcatgcgtg ttttccagcc
     9721 aggggaagat aaattaaatg acgataccga agtaattgaa gggctcgata atccggtcat
     9781 tgatgcccgc tttccggatc tggctgaagc cactcgccag gaaattgaat tgatacgtgg
     9841 tgcctcctcc gcattcgacc gtgcggagtt cctctccggc aagcagactc ctgtattctt
     9901 cggctcggcg ataaacaact tcgggatacg cgaggttctt gacgcgttgg tcgagcttgc
     9961 gccgccccca cgatcgcgca gcgcttttca acgccaggtg caacctggcg aaacccgatt
    10021 ttccggagtg gttttcaaga tacaggccaa tatgaatccg gcgcatcggg accgtattgc
    10081 ttttgtacga gtctgctctg gaagattcga gcgcggcatg aatctgagag tggcgcgcaa
    10141 cggaaaggac ttacgcacaa actcggtcgt atcgttttta tcccagcggc gcgagttgct
    10201 ggagaccgct tatcccggcg atatcatcgg gattccaaac catggaacgc tgcaacttgg
    10261 agatacgctc acggagggtg aatgccttca atttacaggc ttgccttttt tcgcaccgga
    10321 aattttccag acggttgaaa tcgccgatcc catg
//
LOCUS       DASSHJ010000217        13296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_577342, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000217 DASSHJ010000000
VERSION     DASSHJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577342"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1918)
                     /locus_tag="VFI43_08490"
     CDS             complement(224..1918)
                     /locus_tag="VFI43_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET7062199.1"
                     /translation="MKDPAHDLSAGSASNFIRSIIEEDNRTGKWNGRVETRFPPEPNG
                     YLHIGHAKSICLNFGLAREYKGICHLRFDDTNPEKEEQEYVDSICDSVRWLGFDWGDH
                     LHYASDYFDQLYRFAEYLIEQGKAYVDSLAAEEIRALRGTLTEAGENSPYRDRTVEEN
                     LDLFRRMKAGEFADGEHVLRARIDMTSPNINLRDPVLYRIRHVSHHRSGDKWCIYPMY
                     DFTHCISDALEKITHSLCTLEFEDHRPLYDWVLDQLSEVLPWHPQQIEFARLNLTYTV
                     MSKRKLIELVDRGIVDGWDDPRMNTLAGLRRRGYTPESIRLFAERIGVSKADSWIDMG
                     VLEDCLREDLNERSQRRLAILDPLKVVIENYPEDRVEDCWAPNHPQKPEAGNRALPLS
                     REIYIERSDFMETPSKGYFRLSPGREVRLRYAYIIQCTDMVKNSNGEIVEIRCTYDPD
                     TKSGTPGADKRKVKGNIQWLSAACARPAEVRLYDRLFTDAYPGAGGTDYLSFLNPDSR
                     KTITALVEPSLGSVQPEERFQFERHGYFVADRRDTVPGNLIFNRAVTLRDSWAKPG"
     gene            2006..2992
                     /locus_tag="VFI43_08495"
     CDS             2006..2992
                     /locus_tag="VFI43_08495"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET7062200.1"
                     /translation="MKPLTFSILRLLSDSKFRSGETIAKDLGISRSSVSYALRELDET
                     GVTVQKVHGRGYRLCEPVQWLNRDAIMMHLGREAQGFNLEIFDIVESTNSLLFQKIPG
                     LAFQNNPAHVIAAELQTRGRGRRGRQWHSGLGNALTFSLLWQFQQGASLLSGLSLTVG
                     VAIIRAFKSNGIEGVMLKWPNDVIFNFCKLAGILIELQGDMLGPTTAVIGVGVNLDLP
                     RNVKARIDQAATGVSSINGVMPDRNKLLAALLVEIADALKEFERHGFSAFREEWTNYH
                     VFEKKHVTLRLPDGTDLKGLADGVADNGSLLLRTAEGMRGYSSGEISLRPNT"
     gene            2992..3747
                     /locus_tag="VFI43_08500"
     CDS             2992..3747
                     /locus_tag="VFI43_08500"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET7062201.1"
                     /translation="MKSFLLMVDSGNTNVKWGLYDGDGQKWLKRGVVPQGDTKSLERG
                     WKGLQEPSRIIVSNVANAQAKENLSELFSSWSIEPCWVSACPYQCGVRNYYEDASQLG
                     SDRWAALIATWQQQRQGCLVVDAGTATTIDALSDTGEFLGGIITPGMDLMQKALTENT
                     GALKLEGGKFRDYPDRTADAIYSGAVQAIAGAIERMAALLNSTLGREPKCILSGGAAK
                     QLQPHLHVSTTVVDNLVLEGLAVIAQEIPEAQP"
     gene            3802..4539
                     /gene="yihA"
                     /locus_tag="VFI43_08505"
     CDS             3802..4539
                     /gene="yihA"
                     /locus_tag="VFI43_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET7062202.1"
                     /translation="MSVFHKAAFYTTVNRIADLPPANGIEIAFAGRSNAGKSSAINSL
                     ARQGRLAFVSKTPGRTQHINFYQLGGGRFLVDLPGYGYARVPAPIRNHWRQLISTYLQ
                     TRTSLHGMVLIMDARHPLTPLDRQMLAWYAATQKPVHILLAKSDKLSRQQAENTLKEV
                     GAFLAENYPQCTVQLFSSLTGKGVDEASAVLCAWLGMQPDGIGLGGMKTQEMESGAGK
                     NPVKPVAKTPEIKNPRLKGNKTGGKLP"
     gene            4672..5676
                     /gene="hemB"
                     /locus_tag="VFI43_08510"
     CDS             4672..5676
                     /gene="hemB"
                     /locus_tag="VFI43_08510"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7062203.1"
                     /translation="MPASSQFPEKRMRRMRRDEFSRRLMRENHLRPDDLIYPVFVLDG
                     PSREELVPSMPGIKRQSLDLLLYQAENCLKLGIPALAIFPVIDPSLKNLNATEALNPK
                     GLVPRTVSELKKRFPELGIITDVALDPYTSHGQDGLIDESGYVMNDETVNVLAQQARV
                     HAQAGADIVAPSDMMDGRVAAIRAALEGAKCIHTRILAYSAKYASSFYGPFRDAVGSA
                     ANLGTGNKYTYQMDPANSDEALWEVRLDLEEGADMVMVKPGLPYLDIVRRVKEQFGVP
                     TFVYQVSGEYAMLKAAAQNGWLDEKACVLESLLSFKRAGADGILTYYAMTAAEWLRTA
                     "
     gene            6097..6600
                     /locus_tag="VFI43_08515"
     CDS             6097..6600
                     /locus_tag="VFI43_08515"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7062204.1"
                     /translation="MSGHDQSLQDLIEGIRFAMFTTRSHDGALHSRPMTTQDVDADAS
                     DAGERLWFFMSRSSEPVSELNTDPKVNVSYADLGKDAYVSVSGIARLVEDAAIKQRLW
                     SKMNEIWFPKGVEDPDLALVCVEVDEAEYWNVKESKLVQMAKMAKAAITGKRPTDIGE
                     HVRVRGD"
     gene            complement(6621..7211)
                     /locus_tag="VFI43_08520"
     CDS             complement(6621..7211)
                     /locus_tag="VFI43_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062205.1"
                     /translation="MESRNGAGRPGRSIFGKAMRSGTVAGLATAVVATLAGKRETGSY
                     AAALNATSHIIWGDRAGKQNAPSLKYTLTGFLLDYASGIFWALLYEKLFGRAPVHRGR
                     TIGLQKVRSPIPSRDGSLHQVQLTNRILGAGAVTAAAYIIDYHLIPKRFTPGFEKRLS
                     GKSLAAVFVALGAGLVARDVIAAAKAKSGGNKKRIR"
     gene            complement(7536..9857)
                     /locus_tag="VFI43_08525"
     CDS             complement(7536..9857)
                     /locus_tag="VFI43_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET7062206.1"
                     /translation="MLARRWVYALAAFLGLALLGILLAGFAALVTIPTLPSLEVLTDY
                     RPKIPLRVYSAEGLLIGEFGEERRELVKIAAAPQSLKQAIVAAEDDRFYQHGGVDYVG
                     ILRAAYSNFTSGGMRQGASTITMQVARNFFLTKEKTLTRKFSEALLAFKIEHSLSKDE
                     ILELYFNQIYLGQRAYGFAAASQVYFGKPLREINLAEAAMLAGLPKAPSRFNPVVNPK
                     RAKTRQLYVLRRMHELGYITDEEFKLAEKTPVRTKRESQEFAMRADYVAEMARQAVHE
                     RFHDDAYTRGFKVYTTIRRGDQDAAYRAVRQGVIEYDRRHGYRGPEAFIQLPKNNGDL
                     EEFLEDALQEVSDSDDIQAAVVLAADPKIVRAYRRGGETIEITGDGLKFAQRFLDSKH
                     SSKQRIQPGSLIRVRSNDKGAWQIVQLPLVEASLVSLDPHDGAIRAMVGGFDFSRNQF
                     NHVTQAWRQPGSSFKPFIYSASLEKGFTPASVIEDAPLSFTAEETGSDQWEPRNFDGN
                     YDGPLRMREGLVKSKNLMSIRLLQGMGVQYAQDYVARFGFDPKQHPAYLPMALGAGSV
                     TPIQMAVGYAAFANGGFRVSPYFIQRIEDEKGRTVEQTKPVRAGQGAKQIIDPRNAFL
                     MTSMMQDVVQRGTATKAKQLGRSDLAGKTGTTSNYMDAWFCGYQPDLVAVAWIGFDTP
                     RSLGNNETGGHAALPIWINYMGKTLKGVPMGSYSPPPGITQARINPATGLREPEGTMN
                     EYFFEEQLPPEAENKIEEVLKSVEDVIKEFFTQ"
     gene            complement(10154..10534)
                     /locus_tag="VFI43_08530"
     CDS             complement(10154..10534)
                     /locus_tag="VFI43_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HET7062207.1"
                     /translation="MSYAAIKMIHITSVVISYLLFSLRSIWMIRGSPTLKRRWVKITP
                     HVVDTVLLASAVTLAVWIQQDPIHDSWLSAKVAGLLAYIGLGMMALRFGKTRKTKILY
                     WIAAQVAFFYIVLVAVTKNPSLLF"
     gene            complement(10550..10954)
                     /locus_tag="VFI43_08535"
     CDS             complement(10550..10954)
                     /locus_tag="VFI43_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062208.1"
                     /translation="MVTTILIPAMAQMNSASERIGFFGQIESRFAPQARFTTLLVGLS
                     GFYMVYVLDAWGRFAELRFWWMHAMVFVWVIFTLMLFLVEPFVLRRQLNNAADRDPAK
                     TFRRMKRMHWILLSISLVAAAGAVAGSHGWMM"
     gene            11401..11724
                     /gene="trxA"
                     /locus_tag="VFI43_08540"
     CDS             11401..11724
                     /gene="trxA"
                     /locus_tag="VFI43_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HET7062209.1"
                     /translation="MSHIHAVSDGTFEAEVLQSGMPVLVDYWAEWCGPCKMIAPILDE
                     VAKEYGNRLKIAKLNIDENQATPSKYGIRGIPTLMLFKNGNIEATKVGALSKSQLTAF
                     IDSHI"
     gene            11867..13126
                     /gene="rho"
                     /locus_tag="VFI43_08545"
     CDS             11867..13126
                     /gene="rho"
                     /locus_tag="VFI43_08545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET7062210.1"
                     /translation="MHLSDLKNFHVTELVEMAIANEIDGANRLRKQDLIFALLKNQAR
                     KGESIFGEGTLEVLQDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNSEPPEHSKHKILFENLTPLFPTDRLQLERDIKAEENVTSR
                     IIDLIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIAANHPDVILMVLLIDERPEEVTE
                     MIRSVRGEVISSTFDEPAVRHVQVADMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMAEKRIYPAINVNRSGTRREELLIKPDVLQKIWVLRKLLYPMDD
                     MEAMEFLLDKIKATKNNADFFDSMRRV"
BASE COUNT         3142 a         3638 c         3490 g         3026 t
ORIGIN      
        1 gaggcatcag agggaaagcg acagacgtcg gagtgcatgg tgcttgctct tctttacgac
       61 gaaaaggcga ggcggagcat cgatcttcca gcgattattt ctggagagtc gaaaccagca
      121 gagctcgagc cagtgatatg aagtccttct aacatccttc tagcatcctt cggagcaggt
      181 ccagttctgg cggcgcttcc cacggtcatc gatcccctgg tactcagcct ggcttcgccc
      241 aggaatccct gagtgtcacc gcccgattga atatcagatt gcccggtacg gtatctcttc
      301 tgtcggcaac gaaatacccg tggcgctcga actggaagcg ctcttccggc tggacgctgc
      361 caagcgaagg ctccacgagc gctgtgattg tttttcttga atcggggttc aaaaagctta
      421 agtagtcggt acctcctgcg ccaggatacg catcggtaaa caggcgatca taaagccgga
      481 cctcggctgg acgcgcgcat gcggccgaga gccactggat atttcccttg accttgcgtt
      541 tgtcggctcc cggcgtcccg ctctttgtat cggggtcgta cgtgcagcgg atttccacaa
      601 tttctccgtt gctgtttttt accatatccg tgcattggat tatataagcg tagcgaagcc
      661 ggacttccct tcccggcgac aggcggaaat agcccttcga aggcgtttcc atgaaatcac
      721 ttcgctcgat gtaaatctcc ctggacagtg gcagagccct atttcctgct tcgggctttt
      781 gggggtggtt gggcgcccag caatcctcca cccggtcttc gggataattt tcaataacga
      841 ctttcagtgg atccagaatc gcgagccgcc gttgcgaacg ctcgttcagg tcctcgcgaa
      901 gacagtcttc gagcaccccc atatcgatcc atgaatccgc cttgctgacc ccgatacgtt
      961 ctgcgaagag ccggatcgac tccggcgtgt accctcgtcg tcgcaatccc gcgagcgtgt
     1021 tcatgcgcgg gtcgtcccag ccgtccacga tcccgcgatc gaccagttcg atgagcttac
     1081 gcttgctcat caccgtgtag gtaagattga ggcgggcgaa ttcaatttgc tggggatgcc
     1141 acggcaggac ttcggataat tgatccaaaa cccagtcgta gagggggcgg tgatcctcga
     1201 attccagcgt gcagagggag tgcgtgatct tctccagcgc atccgaaatg caatgcgtga
     1261 agtcatacat cgggtagatg caccacttgt ctccgctgcg gtgatgagaa acgtggcgga
     1321 tgcggtagag aaccgggtcc cgaagattga tgtttggaga agtcatgtcg atcctcgcgc
     1381 gcagcacatg ctcaccgtcg gcgaattccc ctgccttcat gcgccgaaaa aggtccaggt
     1441 tttcttcgac ggtgcggtcg cggtacgggc tgttttcgcc cgcttcagtc aacgtccctc
     1501 gcaacgcccg gatttcctca gcggcaaggc tgtcgacata tgccttgccc tgttcaatca
     1561 ggtattccgc gaaccggtaa agctgatcga aatagtccga tgcatagtgc aggtgatccc
     1621 cccaatcaaa gccgagccat cggacagaat cgcatatcga gtcgacgtat tcctgttctt
     1681 ccttttccgg attcgtgtca tcgaagcgca ggtggcatat ccccttatac tcgcgcgcca
     1741 gcccgaaatt gaggcagatg cttttagcat gaccgatatg cagatagcca ttaggctcgg
     1801 gcggaaaccg tgtttcgact cgtccgttcc acttgcccgt acgattatct tcttcaatga
     1861 tgctgcgaat gaaattcgaa gcggagccag cggacaggtc atgcgcagga tctttcaagt
     1921 gaacctctct taggtagacc ggatattata atggctctgc ctgattcgac acattacgcc
     1981 gggtatttac actacactgg ttagcgtgaa gcctctcact ttttccatcc tgcgcctttt
     2041 gagtgatagc aaattccgct cgggcgaaac cattgcgaag gatttgggaa tttcccgctc
     2101 aagcgtgtcc tacgcgcttc gcgaactgga tgaaaccgga gttacagtac aaaaggtgca
     2161 cggacggggc taccggctgt gcgaaccggt acaatggctg aacagggacg ccatcatgat
     2221 gcatcttgga agagaggcac aaggctttaa ccttgaaatt ttcgatattg tcgaatcgac
     2281 caacagtctc ctgtttcaaa aaattcccgg actcgctttc cagaacaacc cagcccatgt
     2341 gattgctgcc gaactgcaga ccagagggcg tggccggcga ggccgacaat ggcattccgg
     2401 actggggaac gccctaacgt tttccctgct gtggcaattt caacaaggtg cgagcctact
     2461 ttcaggtttg agccttacgg ttggcgtggc aatcatacgc gcattcaaat ccaacgggat
     2521 cgaaggcgtt atgctcaaat ggcccaacga tgtgatattc aatttctgca agctggctgg
     2581 catactcatc gaattacaag gagatatgct aggcccgacg actgcagtca ttggcgtcgg
     2641 agtcaacctc gatttaccca ggaatgtaaa agcccggatc gaccaggcag cgactggcgt
     2701 gtcgtccata aatggagtta tgcctgaccg taataaactg ctagccgcat tgctggttga
     2761 gatcgccgac gctttgaaag aattcgaacg ccatggcttt tcagcattcc gggaggaatg
     2821 gacgaactat cacgttttcg agaaaaagca tgtcaccctt cggcttcccg atggtaccga
     2881 cctcaaggga ctcgccgacg gcgtagccga caatggatct ctgctgttga ggaccgccga
     2941 aggcatgcga ggatacagca gcggagagat ttcgcttcgc ccgaacacgt aatgaaatcg
     3001 ttcctgctga tggtggattc aggcaatacc aacgtcaagt ggggactgta tgatggtgat
     3061 gggcagaaat ggctgaagcg cggcgtagtc cctcaaggtg acacgaaatc cctggaacgc
     3121 ggatggaaag gcctgcagga accgtcccgg attatcgttt cgaacgtggc aaatgcacag
     3181 gcaaaagaaa acctttctga attattttcc tcctggtcaa tcgagccttg ctgggtgagc
     3241 gcgtgccctt accagtgcgg tgtccgcaac tactatgaag acgcctccca actcggcagc
     3301 gaccggtggg ctgcactgat tgcaacctgg cagcagcagc ggcagggctg tctcgttgtg
     3361 gatgccggca ccgctacgac tattgatgca ctctcggaca ccggagagtt tctgggaggg
     3421 atcatcactc ctggaatgga tctaatgcag aaagcgttaa cggaaaatac cggcgccctg
     3481 aaactggagg ggggaaaatt tcgcgattat cctgatcgca ctgcggacgc catctacagc
     3541 ggtgccgtac aagcgatcgc aggcgctatt gagcgtatgg ccgcgctgct caattccacc
     3601 ctcggtcgcg agccgaagtg tattttaagc ggaggcgctg cgaagcagtt gcaaccccat
     3661 ctccacgtga gcacgaccgt tgtggataat ctcgtgctcg agggcctggc ggtcatcgcc
     3721 caggaaattc cggaagcaca gccgtagctc tcccggacag tgataccgtg ccctttggtc
     3781 agggcgacaa gttgatagcg tatgagtgtt ttccataaag ccgcgtttta taccaccgtt
     3841 aaccgaattg ccgaccttcc cccggcgaat ggaatagaaa ttgcttttgc gggtcgttcg
     3901 aacgcaggca aatcgagcgc gatcaattcg cttgcacggc aaggacgtct tgcctttgtc
     3961 agtaaaaccc cagggcgcac ccagcatatc aatttctatc agcttggagg aggacgtttt
     4021 ctcgttgatc ttccaggcta tgggtacgcg cgggtcccgg ccccaatacg caatcattgg
     4081 cggcaactga tcagcacgta tctgcaaacc cgcacctcgc tacatggaat ggtcctcatc
     4141 atggatgcac gacaccctct cacccctctt gaccgtcaga tgcttgcctg gtacgcggcc
     4201 acgcaaaagc ccgtacatat actgctggca aaatcagaca agctttcgcg gcagcaagcg
     4261 gaaaacaccc tcaaggaggt gggcgccttt ttggctgaaa attatcctca atgtaccgta
     4321 caattgtttt ccagcctgac tggaaaaggc gtggatgaag cttcggctgt gctatgcgcg
     4381 tggctgggca tgcaacccga tggaatcgga ctcggtggaa tgaaaacgca ggaaatggaa
     4441 agcggcgcag gtaaaaaccc ggtaaaaccg gttgcgaaaa cgccagaaat aaaaaacccc
     4501 cggttaaagg ggaataaaac cgggggcaaa ctaccttaat tagggattaa ggtacccgct
     4561 cagggaggaa aagcgggaga caaatacaca acgtctggca ataagaaaag aaatttgagt
     4621 ataagttccc gtttttttat ttttttacca tttttattgt aaaacttttt tatgcctgct
     4681 tcgagtcagt ttcctgaaaa aagaatgagg cgcatgcgcc gcgacgagtt ttcccgcagg
     4741 ttaatgcgtg agaatcacct gcgaccggac gacctgatct atccagtatt tgtgctcgat
     4801 gggccgagcc gtgaagagtt ggtgccctca atgcccggta tcaagcggca aagtcttgat
     4861 ttgctgctgt atcaggctga aaactgcctg aagctgggca taccggcgct cgcaatcttt
     4921 ccggtcatcg acccgtctct aaaaaatcta aacgctacag aagcgctcaa tccaaagggt
     4981 ctcgtaccgc ggacggtcag tgaactgaag aaacgctttc ccgaactcgg gatcatcact
     5041 gacgtggccc tcgaccctta caccagtcat gggcaggacg gtttgattga tgaaagcggt
     5101 tacgtcatga atgacgaaac ggtgaatgtt ctcgcccaac aggcacgtgt ccatgcacaa
     5161 gcaggcgcgg atattgtggc cccttcggac atgatggatg gccgtgtggc ggcaatccgc
     5221 gcggctctcg aaggggcgaa atgcattcat acacgcattc ttgcctactc ggccaaatat
     5281 gcatcgagtt tctacggccc cttccgggac gcggtcggct ccgccgccaa tctaggtact
     5341 ggcaacaaat acacatatca gatggatccg gcaaattccg acgaagcgct ttgggaggtg
     5401 cgactggatc tcgaggaagg cgccgatatg gtgatggtga agcctggact cccttatctg
     5461 gatatcgtac gtcgcgtaaa ggaacagttc ggagtgccaa cttttgtgta ccaggtgagt
     5521 ggagaatacg ccatgctgaa ggccgccgcc cagaacggct ggctcgatga aaaagcgtgc
     5581 gtgctcgaat cactgctgtc tttcaagcgg gcaggcgcag atggcattct gacttattat
     5641 gcgatgacag cggcggagtg gctaagaact gcctagtact ttcccaggat gggcccggta
     5701 caaacctgat tgaggcaagc gtgccaatac gccagcctat ttttccttta gggccggcgg
     5761 aaaaacgatg cccttccaag tcattttcct ggttgtatcc cttgtctcac tcaggagtag
     5821 tggttgacga cataggctcg gatcgtcttt cataatgccg agtgccactt tcagggcaat
     5881 cccaaaagca tcgatagtta agattcaagc tggagggcaa agaccgtgct cgtaaatgcc
     5941 tgcaaggcgg ctgcggggct tgggtgtatg gatgatgaat gttcatgcgc tgggtggaag
     6001 aaatacgctt gctggagcac gtggagctgt cccctcggct ttctcttttg ctatattgca
     6061 attacaacaa ctgatttaaa gactcaagga gaaacaatgt ctggccatga ccaatcgctc
     6121 caagacctga tcgaaggcat ccgtttcgcc atgttcacca ctcgcagtca tgacggcgct
     6181 ctgcattcac gacccatgac cactcaagat gtagacgcag acgcttcaga tgcaggcgaa
     6241 cgcctgtggt tctttatgtc ccgctcgagt gaacccgtaa gtgaattgaa tactgatccg
     6301 aaggtcaacg tcagctacgc cgatctcggc aaggatgctt atgtttccgt gtctggcatc
     6361 gcgcggcttg tggaagacgc ggcgataaaa cagcgcctgt ggtcgaaaat gaacgaaatc
     6421 tggtttccga agggcgtcga ggatccggat cttgcgctcg tctgtgttga agttgatgag
     6481 gctgagtatt ggaatgtcaa ggagagcaag cttgtgcaga tggcgaaaat ggcgaaagcg
     6541 gcgatcacgg gcaagcgccc aaccgatata ggagaacatg tccgggtgcg aggcgactga
     6601 gccgtggacg taagcaggag tcatcgaatc ctctttttgt tgcctccaga ctttgccttt
     6661 gccgctgcaa ttacatcccg cgccacaagt cctgctccca gggccacgaa tacggcagca
     6721 agtgattttc ccgaaagacg cttctcaaat ccgggtgtaa agcgcttggg aatgagatgg
     6781 tagtcgataa tatacgccgc cgctgttacc gcgcccgcgc cgagaatccg atttgtcaac
     6841 tggacttgat gaagcgagcc atcacgtgaa ggtataggtg aacggacctt ttgaaggcca
     6901 atagttcgac cgcgatggac aggagcacgt ccaaataatt tttcatacag caacgcccag
     6961 aatatccctg atgcgtaatc aagcaaaaag cccgtcagcg tatatttaag tgacggagcg
     7021 ttctgtttcc ccgcacgatc cccccagatg atatggcttg tcgcgttgag ggcagcggcg
     7081 taagaacccg tttcgcgctt tccagccaac gttgcaacaa ccgcagttgc caaacctgct
     7141 acagttccag acctcatcgc ctttccaaaa atacttcgcc ccggcctgcc tgctccgttt
     7201 cttgattcca tgggtttttt atccgcggtt attattcact ctatccgttg cgatatcagg
     7261 cgttgaaggc gggagaaagt atcccgtcta agcgatgctc tgcatccatg catcaattct
     7321 cgctacttct ttatttaaat taataagagc ttaagaaaat tgcaagattt gggaggcgat
     7381 tggcagaaga tatttcatca agaaaatgag cagcactccc gcgacaaccg cgaatactgt
     7441 ctggaggcct gaccgtacat gagtgtttgc ctgccagttc ggcgttgcca tcgcatcgag
     7501 cgatcagcag ggggaaaagg ggcaatacat ctcgcctatt gagtgaagaa ttcctttatg
     7561 acatcctcca cggacttcaa cacttcctct attttgtttt cagcttcagg cggcagttgc
     7621 tcctcgaaga aatactcatt catcgtcccc tcgggttccc gcagacccgt cgcaggatta
     7681 atccgggctt gggtaattcc gggaggcgga ctgtacgagc ccatcggaac gcctttcaga
     7741 gttttcccca tgtaatttat ccagatcggc aaggcagcat gcccccccgt ctcattgttt
     7801 cctaaagacc tcggagtatc aaaaccgatc caggcgaccg caacaagatc aggctggtaa
     7861 ccacaaaacc aggcgtccat ataattgctg gtcgtcccgg tttttcccgc caggtcgctg
     7921 cgacccaatt gttttgcctt cgtcgcggtg ccgcgctgca ccacatcctg catcatgctg
     7981 gtcatgagaa atgcgttgcg cggatcaatg atctgctttg caccctggcc agcgcgaacg
     8041 ggtttcgttt gctccactgt gcggcccttt tcatcctcta tccgctggat gaaataaggc
     8101 gaaacgcgaa atcccccatt ggcgaatgcg gcatagccca ctgccatctg tatcggcgtg
     8161 acggatccgg cccccaatgc catgggcaaa taggccgggt gctgcttggg atcgaaaccg
     8221 aacctggcta cgtaatcctg cgcatattga acgcccatgc cctgcaggag gcggatggac
     8281 atcaggttct tggatttgac caagccttcg cgcatgcgta acggcccgtc gtaattacca
     8341 tcgaaattac ggggctccca ctgatcgctt cctgtttcct cggctgtaaa tgaaagcggg
     8401 gcatcttcga tgacagacgc cggggtgaag cctttttcca gcgaggcgga atagatgaat
     8461 ggcttgaagc tcgagcctgg ctgacgccat gcctgggtca cgtggttgaa ctggttccgg
     8521 ctaaagtcaa aaccgccgac cattgcccgg atcgcgccgt catgcggatc aagagacacc
     8581 agcgaagctt ccacgagcgg aagctgaacg atctgccacg cacccttgtc attagaccgc
     8641 acccgtatca gcgagcccgg ctgaatgcgc tgtttgctgc tgtgcttgct gtcgagaaac
     8701 cgctgcgcaa acttgaggcc gtcgccagtg atttcaatgg tttctccccc cctccggtac
     8761 gcacggacaa ttttcgggtc ggctgcgagc acgaccgccg cctggatatc gtcgctatcc
     8821 gatacttcct gtagcgcatc ttccagaaac tcttccagat ccccgttatt ttttggcagt
     8881 tgtatgaatg cctcaggccc ccggtatccg tggcgtctgt cgtactcgat cacgccctgg
     8941 cgcactgcgc ggtaggccgc atcctggtcg ccgcggcgga tcgtggtgta aaccttgaat
     9001 cccctcgtgt aagcgtcgtc atgaaagcgc tcatggaccg cctggcgcgc catttccgct
     9061 acataatcgg cgcgcatggc aaactcctgg gactcccgct tggttcgcac gggcgttttt
     9121 tccgccagct tgaactcctc gtccgtaatg taacccagct catgcatgcg ccgcagcaca
     9181 tacagctgcc gcgtctttgc ccgcttggga ttgacaacag ggttgaaccg ggaaggcgct
     9241 tttggcaaac cggcaagcat ggcggcctca gcaaggttga tctcacgcag gggcttgccg
     9301 aaatatactt gggacgccgc cgcgaatccg taggcgcgct ggccgagata tatctgattg
     9361 aagtagagtt ccaggatttc atccttgctc aagctatgct cgatcttgaa ggcaagcaga
     9421 gcctcgctga attttcgagt cagtgttttt tccttcgtca ggaaaaaatt tcgcgcgacc
     9481 tgcatggtta tcgtgctggc gccctgccgc atcccacccg aagtgaaatt ggaataggcc
     9541 gctcgcaata tccctacata gtcgaccccg ccgtgctggt aaaaacgatc atcctcagca
     9601 gccactattg cctgcttgag actttgaggc gcagccgcga tcttgacgag ttcgcgccgc
     9661 tcttcgccaa actcgccaat cagaagtccc tcggcgctat agacgcgcag cggaatcttg
     9721 ggacgataat cggtcaggac ttcaagggaa ggcagcgttg gtatcgtgac cagtgccgca
     9781 aagcccgcca gcaagatccc gagcagagcc aggccaagaa aagcggcaag cgcgtaaacc
     9841 caacgacgcg ctagcatggg gaaaagattt ccaaatgaaa actcaatatg tacatatgcg
     9901 attataccaa gccactcccg cgcctgacat ggaaatccgc aaagctaacg tgtgtaaagg
     9961 cgagcccgga tggaagatca tcagaggttc gaggggtatt atcgtgagat taggagtact
    10021 ctgggataat cgaagcccga ttgaagggct tcagaatagg tagccgccgg ctttcgagcg
    10081 tggcaaccga aaggaacagc tccgaaggaa cagtcccgat atgcttgttt tggaaaacag
    10141 ctcagggagc attttagaaa agcagcgacg ggttttttgt aacagcaacc agtacaatgt
    10201 aaaaaaaagc aacctgtgcc gcaatccagt agaggatttt cgttttacgc gttttcccga
    10261 acctcaatgc catcatcccc aggccgatat aagccagcaa tcccgccacc ttcgcgctca
    10321 gccaggaatc atgtatggga tcctgctgta tccataccgc cagcgtcaca gcgctcgcaa
    10381 gcaataccgt atccaccacg tgcggggtta tctttaccca tcgccgcttc aaggtcggcg
    10441 agccgcgtat catccatatg ctgcgcaagg aaaacagcag atagctgatc accacgctcg
    10501 tgatgtgaat catttttatt gctgcgtagc tcatgcctgc tccttacacc tacatcatcc
    10561 agccatgact gcctgccaca gccccggcag cagctacaag gctgatgctc aataaaatcc
    10621 aatgcatccg cttcatccgc ctgaaggtct tcgcaggatc gcggtcggca gcgttattaa
    10681 gctgccggcg cagcacgaac ggttccacca ggaaaagcat cagcgtaaag atcacccata
    10741 caaaaaccat ggcgtgcatc caccagaacc tcaattccgc aaaccggccc cacgcgtcga
    10801 gcacataaac catgtaaaaa ccgctcaagc caaccagaag cgttgtaaat cgcgcctgcg
    10861 gcgcaaaacg gctttcaatc tgaccgaaga agcctatgcg ttcggatgcc gaattcattt
    10921 gtgccatcgc gggtatcaga attgttgtga ccattgcgac gccgcctatc caaaatatca
    10981 caccaagaac atgcaacact ctggcgaaca cgaattcatc cataaaatga cggccttttt
    11041 atctgctggg tttgccttcc ggcgctttcc tgcatcagac cccgctccct cgactaaatt
    11101 caatcttgca tcacagtcat cgaactgtat aatgccgcca acccgcaata tccgcgctgg
    11161 ccgggcgcgg cctccagcca agggattttg ggaatgaaac cgggagaatg gccgggaagc
    11221 ctaagggcac tgttttcaac tgcacctggg taacgcctcc tctcatatat tcgcttccct
    11281 ttcccgcatt ttttcgctag catatagtgg ttgtgccgcc gaattacacg attttcagca
    11341 ttcattttta cgtttcggtc cgcaatattc ccttttacaa tacttttgaa tgagaatccc
    11401 atgagtcata ttcatgctgt ttccgatgga acatttgaag ccgaggtatt gcaatcgggt
    11461 atgcctgtgc tcgtggatta ctgggccgaa tggtgcggac cctgcaagat gatcgcgccc
    11521 attctcgatg aagttgcgaa agagtacggc aaccgcctga aaatcgccaa attgaatatc
    11581 gatgaaaatc aggcgacgcc atcgaagtac ggcatccggg ggatccccac gcttatgctg
    11641 ttcaagaatg gaaacattga ggcaacgaaa gtcggcgctc tgtcgaaatc gcaactgact
    11701 gcttttattg acagccacat ctgaactcgc taacctatcg ctgtctccct aaagcggaat
    11761 ccgttcgatt cgccgcttct aaaaaattct agctccaccc catcagcttc accccaacgt
    11821 tcgaagcgtc ttacgttttc ctgttcttca cccgcgagtt tcgctcatgc atttatccga
    11881 cctcaagaac tttcacgtca ctgaactggt agagatggcc atcgccaacg aaatcgatgg
    11941 cgcaaaccgc ctgcgcaagc aggatctgat ttttgctttg ctcaagaacc aggcgcggaa
    12001 aggtgaaagc attttcggcg aaggcacgct ggaagtgctg caggacggct tcggtttctt
    12061 gcgttccccg gatacgtcgt acctcgcagg ccccgatgat atttatgtct cgccaagcca
    12121 gatcaggcgt ttcaatctgc ataccgggga ttcgatagaa ggcgaaattc gaacccccaa
    12181 ggatggagaa cggtatttcg ccctggtgaa ggtggacaag gtcaacagtg agccgccgga
    12241 acactccaag cacaagattc ttttcgaaaa cctcaccccg ctctttccta ccgaccgatt
    12301 acaactcgaa cgcgatatca aggcagagga aaacgtcacc agccgcatca tcgacctgat
    12361 cgcccctatc gggaaggggc aacgcgggct gctggtcgca agccccaagt cgggaaagac
    12421 ggtcatgctg caacacattg cacatgcaat cgcggccaat catcccgacg tcatactcat
    12481 ggttctgctc attgacgagc gtccggaaga agtgaccgag atgatccgtt ccgtccgtgg
    12541 cgaagtcatc tcctcgactt tcgacgagcc ggcggttcgg cacgtgcaag tcgcggacat
    12601 ggtgatcgaa aaagccaagc gactggtgga gcacaagaag gatgtcgtga tattgctcga
    12661 ctcgattacg cggctggccc gcgcttacaa tacagtcgtc cccgcatccg ggaaggtatt
    12721 gaccggcggc gtggatgcca acgcattgca acgtcccaag cgcttcttcg gcgcggcgcg
    12781 caatatcgaa gaaggcgggt cactcaccat tatcgccacc gcactggtcg atactggctc
    12841 gcgcatggac gatgtgatct acgaagaatt caagggaacg ggcaatatgg aaatccatct
    12901 tgaccggcgc atggcggaaa aacgtatcta tcccgcgatc aatgtcaacc gctcaggcac
    12961 ccgccgcgaa gaactgctga tcaagccgga cgtgctgcag aagatttggg tgctgcgcaa
    13021 gcttctctac ccaatggatg acatggaagc aatggaattc ctgcttgaca agatcaaggc
    13081 aacgaagaat aacgcagact ttttcgattc gatgcgccgc gtttaagtct gccagattct
    13141 ttacacgaat gcagtaggtg cattgttcgc tttacgtcgt ctattgccaa atggggagtg
    13201 attgtaggat aatcgttact ttttccgctc gctgcctcgt tttgccgaac ggaatagctg
    13261 gagccaaatc tatgaaacca ggtattcatc caaatt
//
LOCUS       DASSHJ010000218         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1229880,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000218 DASSHJ010000000
VERSION     DASSHJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229880"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFI43_08550"
     CDS             complement(<1..797)
                     /locus_tag="VFI43_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7062211.1"
                     /translation="MDTKHSAETKGKVVATKTLIQAGVVAFVAVVVVLVWKAADVFLL
                     VFAGILLAVFLHAISRWISQKTHFPEKWSLAIVLLTLALIVAGGIWSIAPEVSSQIDR
                     LLQRIPEAVDQIRQQISKYEWTRKLFEHKEPLKKMTPDGSDIRSNIATVFASTFGALG
                     SFLIFLVLGIFLAISPRTYIDGLIQLAPQTGRPRTKEVLHGIGSALESWLLAKITAMF
                     AVGALTAIGLWLLGIELALLLGILAAILTFIPNIGPILALIPAGLLA"
     gene            complement(1287..2390)
                     /locus_tag="VFI43_08555"
     CDS             complement(1287..2390)
                     /locus_tag="VFI43_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7062212.1"
                     /translation="MLDFFLRIVSLCRKELLAILKDPANRVILIVPSLIESILFGYAA
                     TFDLSNVPYAVLDQSRGVASTEVLARLDSTGIFHRVATLRTQEDVAPVINSRQALMVI
                     HFGPRFEDQLNAGESAPVQLILDGRNSNTSGSAISYVGTIIDSYNVARQGVPALSVEW
                     RAWYNPNLETRWNVLPGLIASLSMIQTLMLSALSVARERENGTFDQLLVTPFTPTEIM
                     IGKAIPTVLVGLVQSTIVLLVTLLWFRIPMAGSLATLYTGLTFFTIASVGIGLSISAV
                     SANMQQAMLYGFMLIMPLILLSGLATPIRNMPQFLQVGTLLNPLRFAIDLVQRVYLEG
                     VGLFEVIHDLVPLVIISALTLPLAAWLFRNRLL"
BASE COUNT          630 a          598 c          663 g          516 t
ORIGIN      
        1 aatgcaagca ggccggcagg aatcagggca aggatagggc cgatattggg aatgaaggta
       61 agaatggcag cgaggatgcc gagtagcagc gcgagctcga tgccaagcag ccaaagtccg
      121 attgctgtca acgcacccac cgcaaacatg gcagtaattt ttgcgagaag ccatgactca
      181 agggcagatc cgatgccatg taatacttct ttggtgcggg ggcgaccagt ttgtggagcg
      241 agctggatca agccatcgat ataagtacga ggacttattg ccaaaaaaat gccgagtacg
      301 aggaaaatca ggaagcttcc gagcgccccg aatgttgaag cgaatactgt cgctatattg
      361 ctgcggatat cggagccgtc cggagtcatt tttttaagcg gctctttatg ttcaaacagc
      421 tttctggtcc attcatattt ggaaatttgc tgccggattt gatcaactgc ttcagggatg
      481 cgctgcagca gccggtcaat ttgcgaggat acctcaggag cgatcgacca gatccctccc
      541 gcaacaatca gcgccagcgt taataaaacg attgcgagag accatttttc aggaaagtgc
      601 gttttttggc tgatccatct gctgatggca tgaagaaaaa cagcgagcag gatgccagcg
      661 aagacaagaa gaaaaacgtc cgcggctttc cacaccaata caacgacgac tgcgacaaaa
      721 gcgacgaccc cagcctgaat caaagtcttc gtggctacca cttttccttt tgtttcggca
      781 gaatgttttg tatccatgct agatgggagg gattgttctc catttggatc agctctttcc
      841 gttagtctcc cggctaccag gttttgccct gcaacaagca acagcgcaac gaaaacaaat
      901 cccggtgaca ggccaatact ttgaacgaca gcggttttag gcttgcgtga ggaaagaaag
      961 tgtcaagatg cggcagaaga gttccaagct caccggtgct gtgatctgcg aatggcaatc
     1021 ttttgcaaag ccgctgaacg acgcgaaaaa gcggtcattc tatttatagg ttagccaaaa
     1081 gaaaacgcaa gccgggcatt gagaatatca aataccgctg ctcaatttaa aatacgggac
     1141 aggtagccgg gtgaacacca tttttgctca ccattttcct gattttggat gatcgccaac
     1201 gtataccaag gcgcgggctt atagcagttt tctggctggg cagcataccg ccctctccta
     1261 ctcgcggact ccccttcctt cagggcttat aaaaggcggt tgcgaaatag ccaggcggcc
     1321 agcggcagcg taagcgcgga aatgatgacc agcgggacaa ggtcatggat aacctcgaac
     1381 aggccgacac cttccaggta aacgcgttgc acgagatcaa tcgcaaagcg cagcgggttg
     1441 agcagggtgc cgacctgaag aaattgcggc atatttcgta tcggggtcgc cagcccggac
     1501 aagagaatca gcggcatgat cagcatgaac ccatagagca tggcttgctg catgttggct
     1561 gaaaccgcgg aaatggatag tccgattcct acgcttgcga tggtgaaaaa cgtgagccct
     1621 gtatagagcg ttgccagcga gcctgccatc ggtatcctga accagagcaa tgtgaccagc
     1681 agcacgatgg tggactgaac caggcccact aaaacagtcg gtatggcttt tccaatcatg
     1741 atctccgtcg gggtaaaggg cgtcaccagt agttggtcaa acgttccatt ttcacgctcg
     1801 cgggcgaccg acagtgccga gagcattaat gtttgtatca tgctcaatga tgcgatgagg
     1861 ccgggcaaga cattccagcg ggtctcaaga ttcgggttgt accatgcgcg ccattcaacc
     1921 gaaagcgcag gcacgccctg acgggcgacg ttgtacgaat cgatgattgt gcccacgtag
     1981 cttatggccg agcctgaggt attggaatta cgtccgtcaa ggatcagctg cacgggtgcg
     2041 gactcgcccg cgttcagttg atcctcaaat cgtgggccga agtgaatgac catgagtgct
     2101 tgccgagagt tgataaccgg ggcgacatcc tcctgagttc gcaatgtcgc tacccgatga
     2161 aatatgcctg tgctatccag gcgggcaagc acctccgtcg aggcaacgcc gcggctctga
     2221 tcgagcactg cgtacgggac attcgagaga tcgaaggtag cggcgtagcc aaacaggatg
     2281 ctttcaatca acgaaggaac gatcaggatt acccggttag ctgggtcctt gaggatggcc
     2341 agcagttctt tacggcacag actcacaatg cgaaggaaaa agtcgagcat ggcttaaccc
     2401 tttaacc
//
LOCUS       DASSHJ010000219         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_577862, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000219 DASSHJ010000000
VERSION     DASSHJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577862"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFI43_08560"
     CDS             <1..963
                     /locus_tag="VFI43_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET7062213.1"
                     /translation="CAAQSAKVIKRQSADKVIVDSTVQEKAIAYPTDSKLLNRSREQL
                     VKLVAQAGMALRQNYNRIAPKLAGKIARYAHAGQYKRMRSHLKKLKTLVGRVWRDVSR
                     QLSKAPEHLKPRIVDLLHKVERLLKQQPKDSHKLYSLHAPEAECIAKGKVRQPYEFGV
                     KVSVVTTHKEGLVVGMQSFPGNPYDGHTLHAALKQVEMLTQRQPREVFVDLGYRGATV
                     PAGVKVYHRKLKRITARLKRDIRRRSAIEPVIGHMKNDGRLRRNWLKGTQGDAFHALL
                     CGCGHNLRMILRKLRLLLALILVRLHWQAADDDKANHRLTAAYA"
     gene            complement(1116..2234)
                     /locus_tag="VFI43_08565"
     CDS             complement(1116..2234)
                     /locus_tag="VFI43_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET7062214.1"
                     /translation="MTQTTSAHKFPLREAHGLVRDLMTPDPRIYWLDFLFHISLGWTA
                     FVVILTSDSFSISQVLAIVVATFAFYRAAIFIHELAHLKKGTFKFFRLVWNLICGIPL
                     LIPSFTYDGVHSDHHKPDVYGTAGDGEYIPFATLRPVAMVGYVMFSFLIPIFFIIRFL
                     VLTPLSYLIPPLRKLVWERASSLTIDMNYIRAPDAIRNDVHWRLQEFSAFVYITAIMS
                     FIALGLLTYKVLVFWYVIAMLVFFINSLRTLGAHAYRNPADRRMEFAEQYLDSINVPG
                     HFFVTALWAPVGLRYHATHHLFMSMPYHNLGKAHRRLVNGLGDNGLYVTTVRGSMWDA
                     LRHIWSEAAAASRARDNAAGREERVEQLSGVTGHGDNK"
     gene            complement(2362..2913)
                     /gene="rfbC"
                     /locus_tag="VFI43_08570"
     CDS             complement(2362..2913)
                     /gene="rfbC"
                     /locus_tag="VFI43_08570"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET7062215.1"
                     /translation="MRAVPSALPGVVVIEPKVFGDARGFFFESFNQKDFHQAVELDVC
                     FVQDNHSRSVKGVLRGLHYQIRQPQGKLVRVVRGAVFDVAVDIRRSSPNFGRWFGVQL
                     SEDNHRQLWIPPGFAHGFYVLSDTADFLYKTTDYYGPGFERSILWNDPTIGISWPVEA
                     GGQPIVSEKDSRAVILAEAEVFE"
     gene            complement(2910..3806)
                     /gene="rfbA"
                     /locus_tag="VFI43_08575"
     CDS             complement(2910..3806)
                     /gene="rfbA"
                     /locus_tag="VFI43_08575"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET7062216.1"
                     /translation="MTQRKGIVLAGGSGTRLYPVTKAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILVISTPIDTPRFEQLLGDGSEWGLNLQYAVQSSPDGLAQAFIIGRQFIGN
                     SPSALVLGDNIFYGHDLHMQLRRAMARQGGATVFAYHVHDPHRYGVVAFDSDDRVTSL
                     EEKPAQPKSNYAVTGLYFYDNQVVDIAATLQPSARGELEITDVNRIYLERSQLSVEIM
                     GRGYAWLDTGTHESLIEASNFIETIEHRQGLKVACPEEIAYRQGFITPAQLEKLAEPL
                     VKNGYGQYLLRLLKERRVNESQ"
     gene            complement(3793..>4398)
                     /locus_tag="VFI43_08580"
     CDS             complement(3793..>4398)
                     /locus_tag="VFI43_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HET7062217.1"
                     /translation="STDYVFDGSGIKPWVETDPTGPLNVYGSTKLEGEKAIVDSGCNH
                     LIFRTSWVFARRGENFAKKMLYLAREKDRLTIVDDQVGAPTGADLLADVTAHVVRTAL
                     KQRGVGGVYHVATSGEASWWDYARFVLDFARQTGRKLSVAPADVLPVSADAFHSPARR
                     PQNSRLNITKLQTTFGLTLPPWQTGVSRMLTEILENQYDPA"
BASE COUNT         1058 a         1172 c         1235 g          933 t
ORIGIN      
        1 tgtgcggctc aatcggccaa ggtgatcaag cggcagagcg cagacaaggt cattgtggac
       61 agtacggtac aggagaaggc cattgcctat cccacggaca gcaagctatt gaaccgttcc
      121 cgggaacaac tggtgaagct cgtggcgcaa gccggcatgg cgctgcgcca gaactacaat
      181 cgcatagcgc ccaagctggc aggcaagatt gcgcgctacg cccatgccgg acaatacaag
      241 cgcatgcgca gccacttaaa gaagctcaag accctggtgg gccgagtctg gcgcgatgtg
      301 tcgcgtcaat tatccaaggc cccggagcat ctgaagccca ggattgttga cttgctgcac
      361 aaggtggagc ggctgctcaa gcagcagccc aaagacagcc acaagctcta cagcctgcat
      421 gcgccggaag cagagtgcat cgccaaaggc aaagtgcgcc agccttatga gtttggagtg
      481 aaggtctcag tggtcacgac acacaaggag gggctggtgg tgggtatgca aagcttcccg
      541 ggcaatccct acgatggaca taccttgcat gcagctctga agcaggtgga aatgctgaca
      601 cagcggcagc caagggaagt gttcgtcgac ctgggctatc gcggcgccac cgtcccggct
      661 ggggtcaagg tctaccaccg caaactcaag cgcatcactg ctaggcttaa gcgggacatc
      721 cggcgacgca gtgccattga gcctgtgatc gggcatatga aaaatgatgg ccggctgcgc
      781 aggaactggt tgaagggcac acaaggcgac gccttccacg ccctgctctg cggctgcgga
      841 cacaacttgc gcatgattct gagaaagctg cggcttcttc tcgccctgat tttagtccgg
      901 ttacattggc aggccgccga tgacgacaaa gccaaccatc gccttaccgc tgcttatgcc
      961 tgaaaatcga attgttcaga gctgactcag tatcattctt tctcaagcta aggagaaagg
     1021 gctgcagtag acggctgcaa gaaagggcta aagtatttta tttgcggttg cggttgtatg
     1081 cgtaaagctt caggcacgcc ttacgcaagc tcgcttcatt tattgtctcc atgccctgtg
     1141 acgccagata gttgctcgac tcgttcctcg cgtcctgccg cattatctct tgcccgtgaa
     1201 gctgctgcag cttccgacca gatgtggcgt agcgcgtccc acatactgcc gcgcacggtt
     1261 gtaacgtaaa gcccattgtc ccccagcccg ttcacgagtc tccggtgggc cttgcccaag
     1321 ttatggtagg gcatgctcat gaataaatgg tgggtagcat gatagcggag gccgaccggc
     1381 gcccagagag cagtaacgaa aaaatgtccg ggaacattga tcgaatcgag atattgctcg
     1441 gcaaattcca ttctgcgatc ggcaggattc cggtaggcgt gtgcacccaa cgttcgcagc
     1501 gagtttatga aaaagaccag cattgcgatg acgtaccaga aaaccaggac tttataagtg
     1561 agcagcccca aggcaatgaa gctcatgatc gccgtaatat aaacaaaagc ggaaaactcc
     1621 tgcaagcgcc agtgaacgtc gttgcgaata gcatctggcg cgcggatgta attcatatcg
     1681 atcgtaagcg aagaggcgcg ctcccaaacc agcttgcgca gcgggggaat caggtaggag
     1741 agaggggtga gtaccaggaa tcggataata aagaaaatcg gaattaaaaa cgaaaacatg
     1801 acgtatccca ccatggcaac cggacgaagg gtcgcgaagg gaatatattc tccatccccg
     1861 gccgtaccat agacgtcggg tttatgatgg tcgctatgaa cgccgtcata ggtaaaggat
     1921 ggaatcagca gcggtattcc gcaaatcagg ttccagacga gccggaaaaa tttgaaggtg
     1981 ccctttttca aatgggccag ctcatggatg aaaatagcgg cccggtaaaa cgcgaatgtg
     2041 gcgacaacga ttgctaatac ctgggagatg gaaaatgaat cggacgtgag tatcaccacg
     2101 aaggccgtcc atcccaggga aatatggaaa aggaagtcaa gccagtaaat ccgcgggtca
     2161 ggagtcatga ggtcgcggac aagcccatgc gcttccctca atgggaactt gtgtgcagac
     2221 gtggtctggg tcatctggga gagcctcgca gtcgggtttc aggttgaggg tgcggtccgc
     2281 tctaaagtac ttgcgatagt agtgcgaggg gattccattg ttgaagcgct ctttcctgag
     2341 aattcagaca aaaggtcgcg tttattcaaa tacttcagcc tcggcaagga taactgccct
     2401 ggaatccttt tctgaaacga taggctggcc tcccgcctcg actggccatg atatcccgat
     2461 cgttgggtcg ttccataaaa tgctgcgctc gaaaccggga ccatagtaat ctgtggtttt
     2521 atacaggaag tctgccgtat cgctcaagac atagaagccg tgggcgaatc cggggggtat
     2581 ccacaattgc cggtggttat cttcgctgag ttggactccg aaccagcggc caaagtttgg
     2641 cgatgacctg cgaatgtcta ccgcgacatc aaatactgcg ccgcgcacca cccgcaccag
     2701 cttgccctgc ggttggcgga tttggtagtg caggccacgc aacactcctt tcaccgatcg
     2761 gctatgattg tcctgcacga agcagacgtc aagctcgaca gcctgatgaa aatccttctg
     2821 gttaaagctc tcgaagaaaa acccgcgggc atcgccgaaa accttcggct cgataaccac
     2881 cacgcctggg agcgcgctag gaactgctct cattggctct cattgactct tcgttctttc
     2941 aacagccgca gcagatattg tccgtaacca ttcttgacga gaggctcggc caacttctcg
     3001 agttgtgcag gggtaatgaa cccttggcga taggctatct cctccgggca ggcgaccttg
     3061 aggccctgcc ggtgctcaat ggtctcgata aaattactgg cttcgatcag gctttcatgg
     3121 gtacctgtgt ccagccacgc atatccccgt cccatgattt cgacgctgag ttggctacgt
     3181 tcgaggtaaa tccggttgac atcggtgatc tcgagttccc cccgggcaga tggctggaga
     3241 gtggcagcaa tgtccactac ctgattgtca taaaaataca aaccggtcac ggcatagttg
     3301 cttttcggtt gggctggctt ctcttcgaga gaggtaaccc ggtcgtcgga atcaaatgcc
     3361 acgacaccat accggtgagg gtcatggacg tggtatgcaa atacagtggc gcctccctgc
     3421 cttgccatcg ctcgccggag ctgcatgtgc aaatcatgcc cgtagaaaat gttatcgccc
     3481 agcacgagcg cactagggct gtttccaata aattgtctgc cgataataaa ggcctgggcc
     3541 aagccatccg ggctcgactg taccgcatac tgcaggttca ggccccactc gcttccatct
     3601 ccgagaagct gctcgaaccg cggggtatca attggggtgg agatgaccag gatatcgcga
     3661 atgcccgcga gcatgagcgt gctcagggga taataaatca tcggcttgtc gtaaatcggc
     3721 aggagctgct ttgaaaccgc cttggttacc ggatacagcc gggtgccgga acctccggcg
     3781 aggacgatgc ctttacgctg ggtcatactg attctcgaga atttctgtga gcatgcgcga
     3841 aacacctgtt tgccaggggg gcaacgttaa gccgaacgtt gtctgtaatt tcgttatgtt
     3901 caagcgcgag ttttgtggac gccgggcggg cgagtggaat gcgtcggcgg aaacaggcag
     3961 gacgtcggcc ggagcaacgc ttagctttct gccggtctgc ctggcgaagt ccagtacaaa
     4021 gcgggcatag tcccaccatg aggcttcgcc actggtggca acgtggtaga cgccccccac
     4081 gcctcgctgt ttcagcgctg tgcgaacgac atgagcggtg acatcggcga gcaaatccgc
     4141 accggtggga gcaccaacct gatcatcaac aatcgtgagg cgatccttct cccgagcaag
     4201 gtaaagcatt ttttttgcga agttttctcc acgcctggcg aaaacccagc tcgtgcgaaa
     4261 aatcaagtga ttgcagcctg agtcaacaat cgctttctcg ccttccagct tggtcgaacc
     4321 atagacattc agtggccctg ttggatcagt ttctacccaa ggtttgatcc cactgccatc
     4381 aaagacataa tcggtcga
//
LOCUS       DASSHJ010000220         6642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1187930,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000220 DASSHJ010000000
VERSION     DASSHJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187930"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFI43_08585"
     CDS             complement(<1..347)
                     /locus_tag="VFI43_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HET7062218.1"
                     /translation="MAISDWPESERPRERLLKNGAESLTDAELLAIFLRTGIAGKSAV
                     DLARELLGRFKGLTGLFAADHGSFCETPGMGPAKYAQLQAVLEMARRALSETLKCGDI
                     LNSPKPVRDYLRLT"
     gene            493..1689
                     /gene="coaBC"
                     /locus_tag="VFI43_08590"
     CDS             493..1689
                     /gene="coaBC"
                     /locus_tag="VFI43_08590"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7062219.1"
                     /translation="MKRVLLGLTGGVAAYKAAELARILVKEGMDVQAVMTESALRFVG
                     VATLQALTGKQVLTRLWDSGVAGGMAHIDLSRATDLILVAPASANFIAKLAHGQSDDL
                     LSTLCLARDCPLMVAPAMNRQMWENPATQRNLAALQGDSILILGPAAGTQACGEVGMG
                     RMLEAQEVAERVLAFFQPKLLENTRVLITAGPTYEAIDPVRGITNSSSGKMGYSIARA
                     ALDAGAEVTLVSGRVCLQPPAGVKFVSVVSAEDMLAAVKDEIPCADVFVSVAAVADYR
                     IKNPSRQKNKKTDRDIVLELTPNPDILQYVSGLPNPPFCVVFAAETENLDQNAEEKRR
                     NKKVPLLVANLVQDTVNSEECALTLFDSGGRRHLAKAPKIEQARHLIKHIADCIKNRG
                     TSGMSW"
     gene            1683..2132
                     /gene="dut"
                     /locus_tag="VFI43_08595"
     CDS             1683..2132
                     /gene="dut"
                     /locus_tag="VFI43_08595"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7062220.1"
                     /translation="MVKIDLKIFDERLRKQMPAYATPGSAGLDLRACIENAMTIEPGE
                     ACLIPTGIAIHLNDPGLAAMILPRSGLGHKHGIVMGNLVGLIDSDYQGQIFVSCWNRS
                     HAPFHLSPMERIAQLVVVPVVQVAFNIVDDFQETQRGANGFGSTGKH"
     gene            2104..2565
                     /locus_tag="VFI43_08600"
     CDS             2104..2565
                     /locus_tag="VFI43_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET7062221.1"
                     /translation="MDLAVPASTSPLLIIFFLGLLFSAPLRAQMPTTQLNISGHALTV
                     EIAHTEAARSQGLMFRRSMGVNDGMLFIFPEAGNFSMWMMNTHIPLSVAFVDEKGIIL
                     NIENMSPRTANAHRSAGAAKYAIETNIGWFAKKKIKPGDRVTGLEKAPPAE"
     gene            complement(2591..3940)
                     /gene="ffh"
                     /locus_tag="VFI43_08605"
     CDS             complement(2591..3940)
                     /gene="ffh"
                     /locus_tag="VFI43_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7062222.1"
                     /translation="MFDNLTNRLSGVIKTLRGEARLTESNIQEALREVRMALLEADVA
                     LPVVKEFIGRVREKAIGKEVINSLSPGQAFIGVVQQELITLMGGGKAEINLSTVPPAV
                     ILMAGLQGAGKTTSSGKLAKWLIDNKKKKVLLSSCDTYRPAAIHQLEILASQTGADFF
                     PVHKGQQPKDIATAALSFARTRFYDVLIVDTAGRLAIDEAMMGEIRELEALLKPIETL
                     FVVDAMQGQDAVNTAKAFSEALPLTGVILTKLDGDARGGAALSVLSITGKPIKFAGVA
                     EKITGLEPFYPERMASRILGMGDVLGLIEEAQRGVDQQEAEKLAKKMKSGKNFDLNDF
                     KMQFQQMRNMGGMSAMMDKLPAQLSQAAQNVKIDDKVMGRTEGIIDSMTPQERAKPEI
                     LKASRKRRIAAGAGVSVQEVNRLLAQYEQAQKMMKMMSKGGMSKMLRSMKGMLPGMR"
     gene            4008..4832
                     /gene="ccsA"
                     /locus_tag="VFI43_08610"
     CDS             4008..4832
                     /gene="ccsA"
                     /locus_tag="VFI43_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET7062223.1"
                     /translation="MSGILAYLIASLMYGLIGWHFWRTRWKGAAKTGASAASNGWERY
                     AILAPLLIHGFTLYESVFAGVGLNFGVGSAVSSIVWLTATIYWLSGFFYKLEGLQALV
                     APVAAVAVLLPLVFPSLRPLANTEFPAFKAHISIAMLAYSLLTIAALHALLMAVVERR
                     LHHPAMPSVLTNLPPLLAMESLLFRVIWVGFILLTLTLISGIVFSEEVFGQPLKFTHK
                     TLFGIISWCVFAALLAGRQFYGWRGRIAIRWTLAGFFILLLAYLGSKFVLEVILRR"
     gene            4942..6249
                     /locus_tag="VFI43_08615"
     CDS             4942..6249
                     /locus_tag="VFI43_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET7062224.1"
                     /translation="MPLYVLLIALAALLILSAFFSLSETSMMAINRYRLKHLVKQGSN
                     AARLTTSLLSNTDKLLGVILLGNNLLNAAAATLVAVIVSLFFSHNELALFFGTVGVTF
                     AILVFSEITPKVIAAAYPERIALASSYVLNPLLKIFSPVVWFVNLFVQALLVILRLNP
                     RAQSETQKISLEELKTLVLEAGHFIRKKHQTVLLNLFDLETVTVDDVMVPRNQIEAID
                     LDDDDEVIRNQVLTCYHTRLPVYRTTLDNVVGIIHVRKVLNQMRSGEITAAILERIMR
                     DPYFIPSGTPLFSQLQLFQENRERVGLIVDEYGDWKGLVTLEDIVEEIIGEFTTHAPS
                     QVGAFIRQADDSVIVEGSSLLRDLNRKLGLHLPLGGPKTLNGLIVEHFEDIPEAGTSL
                     KIAGYPMEIIQTQDRVVKVVRIFSILPPAHEMVENSESDLPTA"
     gene            6350..6526
                     /locus_tag="VFI43_08620"
     CDS             6350..6526
                     /locus_tag="VFI43_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062225.1"
                     /translation="MQRGERAILPQPMRDEALPVVCFVRLLAKGIDHSLRRLSRIPSR
                     KAVVALTRTYSEVP"
BASE COUNT         1454 a         1804 c         1729 g         1655 t
ORIGIN      
        1 gtaagacgca gataatctcg aacaggtttt ggcgaattca aaatatctcc gcatttcagc
       61 gtttcgctaa gcgcccgccg cgccatctcc aataccgcct gtaactgcgc gtatttggcg
      121 ggacccattc ccggggtttc acaaaagctg ccatggtctg cggcaaaaag tccagtcaat
      181 cccttgaagc gcccgagtaa ttctcgtgcg agatcgactg cacttttgcc tgcgatgccc
      241 gttcgcagga atatcgcgag caattcggcg tcggtcaggc tttccgcgcc atttttgagc
      301 aaccgctccc gcggtcgctc ggactcgggc caatctgaga ttgccataac cagctaaatt
      361 tggatgagta agaacggctg ggaactgtaa cagttcaact tgggtggcgt tctctgtaat
      421 aaaataggtt ttcccttcaa attcaatttg ctctcgtata gagcttcccg gcatgcaact
      481 tcaaatccaa taatgaaacg cgttctcctg gggttgactg gaggagtggc tgcttataag
      541 gcggcagaac tggcacgtat tctggttaaa gagggtatgg atgttcaggc tgtgatgact
      601 gaatctgcct tacgtttcgt cggggtcgcc acattgcagg cattaaccgg caaacaggta
      661 ttaaccaggc tttgggattc gggtgttgca gggggaatgg cccatataga cctttcgcga
      721 gcgacggacc ttatacttgt tgccccagcc agcgccaact ttattgccaa gcttgctcat
      781 gggcagtctg atgacctcct ctcgacatta tgtctcgccc gggattgccc attaatggta
      841 gctccggcaa tgaaccggca aatgtgggaa aaccccgcga cccagcgcaa tcttgccgct
      901 ttgcaagggg atagcatcct gatactgggg ccggcagccg gcacgcaggc ttgtggtgag
      961 gtcgggatgg gtcgcatgct ggaagcgcag gaggtggcgg aaagagtcct cgcttttttt
     1021 cagccaaaac tcctggaaaa cacgcgcgta ctcatcaccg cagggccgac ctacgaggcg
     1081 atcgatccgg ttcgcggaat cacaaactca agttcgggga aaatgggtta ctcgatcgcc
     1141 cgtgcggcac tcgacgcagg agcggaagtt accctggtct ccggccgcgt atgcctccaa
     1201 cccccagcag gagtaaaatt cgttagtgta gtgagcgcgg aggatatgct ggcagcagta
     1261 aaagatgaaa tcccttgcgc tgatgttttc gtgagcgttg ccgcggtagc ggactatcga
     1321 ataaagaacc cctcacggca aaaaaacaag aaaacggacc gggacatagt gctggagctc
     1381 acgcctaacc ctgacatcct gcaatatgtg agcggtcttc ccaatccgcc tttctgcgtg
     1441 gtctttgctg cagaaaccga aaacctggat cagaatgccg aggaaaagcg ccgcaacaag
     1501 aaggtgccgt tactggtcgc caatctggtg caggataccg tgaattctga agaatgtgcg
     1561 ctgacattat tcgacagtgg cggtcggcgt catttggcga aggcgccaaa gatcgagcaa
     1621 gcccggcatt tgatcaaaca tattgcagac tgcataaaaa accgcgggac ttccggcatg
     1681 tcatggtaaa aatcgattta aaaatttttg acgagcgctt gagaaagcag atgccggcgt
     1741 atgcgacacc cggctcggca ggcctcgact tgagggcctg tattgaaaat gccatgacga
     1801 tagaaccggg tgaagcgtgt ctcattccaa ctggcatcgc aatccatctc aacgatccgg
     1861 gccttgctgc aatgatactt ccgcgttccg gactcggcca caagcatgga atcgtgatgg
     1921 gaaatctggt ggggctgatc gactcggatt accagggaca gatattcgta tcctgctgga
     1981 atcgcagcca tgcccctttt catttgagtc ccatggagcg gattgcacag cttgtggtcg
     2041 tcccggttgt acaggtggcg tttaatattg ttgatgactt tcaggaaact cagcgcggag
     2101 caaatggatt tggcagtacc ggcaagcact agcccgctct tgattatctt tttcctgggt
     2161 cttttgttct cggcgccgct tcgcgcccag atgccgacga ctcaactcaa tatttccggg
     2221 catgccctca ccgtagaaat tgctcatacc gaagcagcgc gttcacaagg gttgatgttc
     2281 cggcgatcga tgggagtgaa tgatggaatg ctgtttatat ttcctgaagc tggaaacttc
     2341 agcatgtgga tgatgaatac tcacattcca ctgagcgtcg ccttcgttga cgaaaaaggg
     2401 atcatcctca acatcgagaa tatgtcgcct cgtacagcga atgcgcatcg ctctgccgga
     2461 gccgcgaaat acgccatcga aaccaatatc ggctggtttg cgaagaaaaa aataaagccc
     2521 ggtgaccggg tgacggggct tgagaaagcg ccccccgcag aataaggcta tcggatggcg
     2581 ctcggggaca ctatctcatt cctggaagca ttcctttcat gcttcgcaac atcttcgaca
     2641 ttcccccctt gctcatcatt ttcatcattt tctgagcttg ctcgtactgg gccagcaagc
     2701 ggttgacttc ctgcacagag acgcctgctc ccgccgcaat gcgccgcttt cgagatgcct
     2761 ttagaatttc cggtttcgca cgttcctgag gcgtcatgga atcgataatg ccttcggtac
     2821 gccccatgac cttgtcgtca atctttacat tttgtgcggc ctggctcaac tgggcaggca
     2881 gtttatccat catggcgctc attccgccca tgtttctcat ctgctgaaac tgcatcttga
     2941 aatcgttcag gtcaaaattt ttgcccgatt tcatcttttt agcgagcttc tcagcctctt
     3001 gctgatcgac gccccgctgg gcttcttcaa tcagccccag cacgtcgccc atgccaagga
     3061 ttcgcgaagc cattcgctcg ggataaaaag gctcgagtcc ggtaattttt tcggccactc
     3121 cggcaaactt tatcggtttg cctgtgatgc tgagaaccga cagggctgcg ccgccccgcg
     3181 catcaccgtc gagttttgta aggatcacgc ctgtgagagg caaggcttcc gaaaaggcct
     3241 tcgccgtatt caccgcatcc tgtccttgca tcgcatcgac aacaaatagc gtctcaatcg
     3301 gtttcagcag cgcttccagt tccctgattt ctcccatcat ggcttcgtcg attgccagcc
     3361 tgcctgccgt gtccactatc agaacgtcat aaaagcgggt tcgggcaaag cttagcgcgg
     3421 cagttgcaat atctttgggc tgttgtcctt tgtggacggg gaagaagtcc gcgccggtct
     3481 ggctagccag aatttcgagc tgatgtatgg ctgcaggccg ataagtatca caggaagaaa
     3541 gcaatacctt tttcttcttg ttatcgatca gccactttgc cagttttccg ctgctggtgg
     3601 ttttgcccgc gccttgcaac ccagccatga gaataaccgc gggtggaacg gtggacagat
     3661 tgatttccgc ctttcctccg cccatcagag taataagctc ctgctgcacc acgccaataa
     3721 acgcttgtcc cggcgacagg ctgttgatga cttcttttcc aatcgccttt tccctgaccc
     3781 ggccgataaa ttccttaacc accggtaagg ccacatcggc ttcgagcagc gccatgcgaa
     3841 cttcacggag cgcttcctgg atgttgctct cggtcagtcg cgcttcaccc cgaagggtct
     3901 tgatgacgcc agaaagccgg ttggtaagat tgtcgaacat gcaaaatgcc tctcaagaaa
     3961 aattcgtgta gactaaataa acacttcatt ttaatcccgc aggatcaatg tccggtattc
     4021 tagcctatct cattgcctct ctgatgtatg ggctgattgg ttggcatttc tggcgcaccc
     4081 gttggaaagg cgcggctaaa actggcgcca gcgccgcgag caacggttgg gagcgttacg
     4141 ccattctcgc gcctttgcta atacacgggt tcacgcttta cgagtctgtg ttcgccggcg
     4201 tcggtctgaa tttcggtgtg ggaagtgctg tttcctccat tgtatggctt actgccacta
     4261 tttattggct ttccggtttt ttctacaagc tggaagggct ccaagctctt gtggcgccgg
     4321 tagccgcggt ggctgtgctg cttccgctgg tcttcccttc gctgcgccca ttggccaaca
     4381 ccgagtttcc cgcattcaag gctcacatct cgatcgccat gctcgcatac agcctgctga
     4441 ccatcgcagc acttcacgcc ttgctcatgg cagtggtcga acgccgtctg catcatcccg
     4501 ccatgccctc agtgctgacc aatctgccgc ccttgctggc aatggaaagc ttattgtttc
     4561 gcgtgatctg ggtagggttt atcctgctca cgctgacgct catcagcggt atcgttttct
     4621 ctgaagaggt gttcggtcaa ccgctcaaat ttacccacaa gacattgttc gggatcattt
     4681 cctggtgcgt gttcgcggcg ttgctggcag gcaggcaatt ttacggatgg cgcggtcgca
     4741 ttgccatccg atggaccctg gccggattct ttattctcct gctggcttat ctcggcagca
     4801 aattcgtact ggaagtgata ctgcggcgct aaacgtgccc gcaccggcgc cccttgcggc
     4861 tccaagctct tgaaatcccg gagcagacgt cctatgtcac atattctgat atttccgtaa
     4921 aaggtttctc ttttggaaga catgccgtta tacgtgctac tgatagcact ggctgctctg
     4981 ttgattttgt ctgccttctt ttccctctct gaaaccagca tgatggcgat caaccgttat
     5041 cgcctgaagc atctcgtgaa acagggaagc aatgccgctc gtctgactac ctccctcttg
     5101 tcgaacaccg acaagcttct cggcgtgatc ctcctgggca acaacttgct gaatgcggcg
     5161 gcggcaacac tggtggcggt gattgtatcc ctgttcttca gtcacaatga gcttgcactc
     5221 ttcttcggca cagtcggcgt gacgttcgcc atactggtat tcagcgagat caccccaaag
     5281 gttatcgcgg cggcttaccc agagcgtatc gcgctcgcct cgagctacgt gttgaacccg
     5341 ttgctgaaga ttttttcgcc agtggtgtgg ttcgtcaacc tgttcgttca ggcgttgctt
     5401 gtaatactcc ggctgaatcc cagggcgcaa agcgaaacgc agaaaatcag ccttgaagag
     5461 ttgaaaaccc ttgtcctgga ggcgggacac ttcatacgta aaaaacacca gacggtgctg
     5521 ctcaatctgt ttgatctcga aaccgtcacc gttgacgacg taatggttcc tcgcaatcag
     5581 atagaggcca tagacctgga tgacgacgac gaagtgatcc gcaatcaagt tctcacctgt
     5641 tatcacactc gcctgccggt ttatcggaca actctcgata acgtcgtcgg catcattcac
     5701 gtacgcaagg ttctcaacca gatgagaagc ggggaaataa cggctgccat actggaaaga
     5761 atcatgcgcg atccgtactt tatcccctcg ggaaccccct tgttctctca actgcaactc
     5821 ttccaggaaa accgtgagcg cgtagggttg atcgtagatg aatacggaga ctggaaagga
     5881 ttggtcacgc tggaggatat cgtggaagaa attatcgggg aatttacaac tcacgctccc
     5941 tcgcaagtcg gcgcatttat caggcaggct gatgacagcg ttatcgtgga aggcagcagc
     6001 ctgcttcggg acctcaatcg caagctcggc ttgcatctgc cgcttggggg gccgaagacg
     6061 cttaacggac ttatcgtaga gcatttcgag gatattccag aggctggcac aagcctaaaa
     6121 attgccggct atcctatgga aatcattcag acccaggacc gggttgttaa agtggttcgc
     6181 attttttcta tcttgccccc cgcgcacgaa atggtagaga actcggaaag tgacctgccc
     6241 acagcctagg gaacctctga acaagtcttc gtgaagcgcg ttgctggtaa aatcgggatg
     6301 atcacaaggc gcgcgatgca ggtcgtagct tggtctacga tgccaaggag tgcaacgcgg
     6361 tgagcgcgcg attttaccgc aacccatgcg ggacgaggct ttgccggtgg tctgctttgt
     6421 caggctcctt gcgaagggga tcgaccattc gcttcgtcgc ctttcgcgca tcccatcccg
     6481 caaagccgtc gtcgcgctca cccggactta ttcagaggtt ccctagtaaa tcatgcttga
     6541 tacaagcgca taggaattac ggttttttag aggccgatcg aatggctgga tcagatgtct
     6601 tttagccatc tgttgcatga ttctccggtt tttttcattt cc
//
LOCUS       DASSHJ010000221        12580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1230843,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000221 DASSHJ010000000
VERSION     DASSHJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230843"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..95
                     /locus_tag="VFI43_08625"
     CDS             15..95
                     /locus_tag="VFI43_08625"
                     /inference="COORDINATES: protein motif:HMM:NF016619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062226.1"
                     /translation="MMTWSDSGDRNINSIAGAVIPVFTKL"
     gene            395..982
                     /locus_tag="VFI43_08630"
     CDS             395..982
                     /locus_tag="VFI43_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062227.1"
                     /translation="MTITTVKRTGVAIFVLAWALASAFHSATVLAHGKETMEEDSCTR
                     RVGDSMVHLSAYQPQFDASAQYCTEIPKGGETYLVIDLVDQAMREMPIGIRVIKGTSE
                     AEDETVSLQKPSYHPDGVIRGELNLDQGLYTVIITAEGRPPLRYQYPLRVQMINYAQI
                     FRTAVGPLIALLLLALFGYKIMKSDRVQRWRSRRS"
     gene            1078..1659
                     /locus_tag="VFI43_08635"
     CDS             1078..1659
                     /locus_tag="VFI43_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062228.1"
                     /translation="MYQKLLKPALLGLFLSMSLPYAMQAHAHGGLSLADDVCKLTIGP
                     YTMHFTGYQPEATQEKEFCEDIPAIGRTVVALDYIEEALRPLPTEVRIIRDTGTPDVD
                     PAKLDEITILHIPPKVYPNGSITFEYNFPQPGKFVGLVTVGDKQEYVSRFPFSVGEPK
                     GTSPFLLGVLAAAAVAAVVFFLRGRKPAKVSPT"
     gene            1971..2441
                     /locus_tag="VFI43_08640"
     CDS             1971..2441
                     /locus_tag="VFI43_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET7062229.1"
                     /translation="MQSWRVSALMRAGSSSVFSFVFTSVFSPVLKGIAMSGMLVMALA
                     GWLYSAPVSAHAMLTKAEPARRAQLTQPPAQVRLWFNEEVEKDYASLTVTHADKPVTD
                     ARPRLAEDDPRSIVLPMPELSPGKYTVKFRVLSVDGHVVDSSYDFTVKSKTQAK"
     gene            2438..4516
                     /locus_tag="VFI43_08645"
     CDS             2438..4516
                     /locus_tag="VFI43_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET7062230.1"
                     /translation="MIEVVATLFRWLQLASNMILVGACVFLAIAGSFHSPWVGRLQRA
                     LPWLGLILLIGLLGILSTTTAQATGISENAWHPGAWLALMQNTRMGHIWVGRAACAAV
                     VLVIACYLRYSEPARWKYILCATAASITLTVGALASHSAAEDLSVVSILPYALHIIMA
                     SVWFGALPAFLAVCFVCTEPVKKEAHPGLQTEAIARIHALFQSREQAIAAGATALKRF
                     STMALPVMLMVIVTGVIITDRMVDTSYSGLVATRYGWLLDAKIALLGVVLVIAARARS
                     TWLPLLAQAGNSEKAQAGGQKLRKWVSFEFLLAMGIVLLATVVANTVPAKHAFIQNWP
                     YSFRFSLAATWGDPMVNTRVYIGIALLTAAIAAAFYGRRRWSRKVYISAVMALATAAL
                     AVGLPPLAVDAYPETYRKTPVPFDTISIANGSALFAENCVACHGPQGKGDGVMAKSFA
                     KPPVDMLTEPHTAKHTAGDFFHWLTFGIPDTGMPVFADKLTEEDRWDVVNYLHAMSRG
                     YQARLMSPKVKPEQPQPNMGPPNFSYVAQDGSSGTLKDFRGEKNVLLVLFSWPQSRER
                     LQELKNIQAQLAQANTVLLAVPEDDPTPQQMAQLEKLPFPVVTQGAHEIVPSYALFRR
                     TLSKPDLLGEGTLPRHMEFLIDRFGYLRARWIPEADGPEWNTTGMLLQQIAQLNREKE
                     ILPPPGDHVH"
     gene            4624..5115
                     /locus_tag="VFI43_08650"
     CDS             4624..5115
                     /locus_tag="VFI43_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062231.1"
                     /translation="MHKLLAAVLVVTATAVALPAGAHSEQHFDNIDTPHGGQMRMAGP
                     YHLELVTKDKELVLYVTDHGDNNVSTEGGVGKATIQKGKSKEKTAIKLEPAGENMLKG
                     NGEFTLTPETVVVVFVKLPDHEAQSVRFTPLKPKGKSTKKTQPAKSQPSGSGEHDHHH
                     MHH"
     gene            complement(5207..6529)
                     /locus_tag="VFI43_08655"
     CDS             complement(5207..6529)
                     /locus_tag="VFI43_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7062232.1"
                     /translation="MHAANKETSLTAGVSRREVWAWAMFDFANSGYTTVVITAIFNAY
                     FVAVVAQNASWGTFAWTLSLGLSYGLIILTAPVLGAYADAYGAKKQLLFASTVGCVVF
                     TALLYFAGPGDLWLAMTFIVLGNFFFGTGENLIAAFLPELAKGSALGKVSGWGWSLGY
                     LGGLVSLGCSLAYVTWAQERGLPSEQFVPVTMLITAALFALSSLPTFLYLKERALPQP
                     HRGSRSVLSESFARLRDTVRHAGKFRDLARFLVCLVFYQAGIQTVITLAAIYAQQVMG
                     FNTQDAMILVLVVNVTASAGAFAFGNLQDRMGHIPTMALTLVGWIIMVLLAWMAETRT
                     MFWAAANVAGLCLGASQSAGRALVGYFSPNSRRAEFFGLWGLAVKLSSILGPVTYGWV
                     SWISGGDHRLAMLISGVYFVIGLFILSSVNVDRGRDAALADGATALET"
     gene            complement(6719..6997)
                     /locus_tag="VFI43_08660"
     CDS             complement(6719..6997)
                     /locus_tag="VFI43_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062233.1"
                     /translation="MIDTTALDGTYELANILIKKATKDQLAECARLLALNLAHHQIKK
                     GEIPVDETLSFLQTAEPTEEHVNVLMEGMLNLIGVLVNVCGDPGHLKH"
     gene            complement(7492..8943)
                     /gene="rng"
                     /locus_tag="VFI43_08665"
     CDS             complement(7492..8943)
                     /gene="rng"
                     /locus_tag="VFI43_08665"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HET7062234.1"
                     /translation="MGSEILVNVTPQETRVAVIEQGAVQELHIERTSSCGIVGNIYNG
                     RVTRVLPGMQSAFVDIGLERAAFLHLADIWRLRQSEDADKPIEQLLHEGKNILVQVIK
                     DSIGTKGARLSTQVSIAGRMLVYLPQVAHIGISQRIEDETERKSLREKLRHLLPAGEK
                     GGFIIRTMAEAASEQELQTDIEYLHKLWNDIREKSSAAVPGLLYQDLNLSYRVLRDFV
                     NDDTVRILVDSRETYQKMKGFAEVYMAEVMGRIDHYAGERPLFDLYGVEEEIERSLAR
                     RVDLKSGGYLIIDQTEALTTVDVNTGGFVGVRSFDETIFKTNLEAAQAIARQLRLRNL
                     GGIIIIDFIDMEDSEHKNAVLAEFKKSLAKDRTHMTVNGFTALGLVEMTRKRTRESLA
                     HILCETCPTCQGRAKIRTAQTMCYEILRELLRESRQFNAREFRILASQQVIDLLLDEE
                     SQSLAQLADFIAKPISLQVEGTYTQEQYDVILM"
     gene            complement(8973..9599)
                     /locus_tag="VFI43_08670"
     CDS             complement(8973..9599)
                     /locus_tag="VFI43_08670"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7062235.1"
                     /translation="MTHFSTRVYLASRSPRRGELLKQIGVEFDLLLLREAPPRQPDVD
                     ETRLREEPPGDYVCRIARTKAGIGYARVLERGWAHLPVLAADTAVSINQSVFGKPKGA
                     VDAESMLRMLSGRSHQVFTAVAVTAQNGDIHTRVSVSTVEFRRLGDREIVSYLSGPEA
                     YDKAGAYAIQGKAAVFISRISGSYSGIMGLPLYETAQLLEGAGIVLLP"
     gene            complement(9651..10121)
                     /gene="rlmH"
                     /locus_tag="VFI43_08675"
     CDS             complement(9651..10121)
                     /gene="rlmH"
                     /locus_tag="VFI43_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET7062236.1"
                     /translation="MKFLVCAVGHKMPAWMADGFSEYARRMPREACIELIEIKPEKRG
                     EGKSIDQLLNAEAIRIRTVLTPRCLKIAMDECGKQLTTIELAEAIGAWMRNGRDIAFI
                     IGGADGLDAEIKISADQVLSLSNMTLPHGLVRILLAEQLYRAVSLIKGHPYHRG"
     gene            complement(10125..10604)
                     /gene="rsfS"
                     /locus_tag="VFI43_08680"
     CDS             complement(10125..10604)
                     /gene="rsfS"
                     /locus_tag="VFI43_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7062237.1"
                     /translation="MTLAKLVKTIVAALEEIKARDIEILDVSKMTTLCDRMIIASGDS
                     NRQTKAIANNVAEKVKAGGGTVYGIEGEQAGEWVLVDLGDVLVHIMQSAVRSHYNLEE
                     LWAQARKVSTAALGRRKIEPSAEQKDHPEQIEDASPEASLPAPRRRKVAKPGPDDSE"
     gene            complement(10601..11275)
                     /gene="nadD"
                     /locus_tag="VFI43_08685"
     CDS             complement(10601..11275)
                     /gene="nadD"
                     /locus_tag="VFI43_08685"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7062238.1"
                     /translation="MVNVGFRLTGIFGGTFDPIHYGHLRVAEEISEMAGLAEMCFVPA
                     AIPRLRQPPVAGIQHRVEMIRLAIEGNSRFSLDVRETQREGITYSVDTLRQMKQELGD
                     EVFLCFMLGADAFSKLAQWKKWRELFGLCHFIVAARPGHPLLEDTHALPDELRVECEN
                     RWVLNPRALIRVPQGLVFLAPTTLLDISATNIRARISLRKSARYLLPDTTLDYILANQ
                     LYLETQ"
     gene            complement(11323..>12580)
                     /locus_tag="VFI43_08690"
     CDS             complement(11323..>12580)
                     /locus_tag="VFI43_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7062239.1"
                     /translation="IEVPSPFTGTVKEISIKPGDKVSEGTPILRMEVGDENKAAPVPQ
                     PGSTSAAAAAPASTPQPAPSQPSGSDTAPRGGEARAEISRATPPPPARAPKPPPVPLP
                     SSPSSERAFSTAHASPSVRKFARELGVDIGLVKGTGPKSRILQEDVQAFVKAELAKPR
                     GAGTELNLLSWPQVDFAKFGPVQVKPLSRIKKISAANLHRNWVMIPHVTQFDEADITE
                     LEALRKESNETTEKDGVKVTILAFLMRASISALKKFPEFNASLESNSGAESNDGDMNL
                     LIKNYYHIGFAVDTPNGLVVPVIRNVDQKGVIAIGSEMASLAALAREGKLKATDMQGA
                     SFTISSLGGIGGTGFTPIINAPEVAILGVSRAATKPVYRDRTLVPRLMLPLSLSYDHR
                     VIDGATAARFTSHLVEVLADMRRVLL"
BASE COUNT         2705 a         3830 c         3422 g         2623 t
ORIGIN      
        1 gctttggcgg acttctgatg acctggagcg actcgggcga ccgcaacatc aacagcattg
       61 ccggggccgt gataccggta tttaccaagc tttaggagaa gcaccttggg caaccccgat
      121 cggggctgcc caagcgcaac ccgggtatac gacacggata ccgaaacacg gacaccggtc
      181 cgctgacgtc agacgacgaa agtggatcgg tgttttttta agtggatata aatgaagact
      241 agccggggcg caaaatcagc gcattatttg tatcgcagta ttttgtataa tggtcacgct
      301 tttttaagca atccttttaa gcaatcttgt ctttgcatcc agtgacttcc gacagcattg
      361 caatgaagcg ggtcaacccg cttgcagttg agccatgaca atcaccacag tgaagcggac
      421 aggggttgcg atattcgtcc tggcatgggc cctggcgagc gccttccatt ccgcgacggt
      481 gcttgcgcat ggaaaggaaa ccatggaaga agacagctgc acgcgccgcg tgggcgacag
      541 catggttcac ctgagtgcct accagccgca gttcgatgcc agcgcccaat actgcaccga
      601 aattcccaag ggcggagaga cctatctcgt gatcgacctg gtcgaccagg ccatgcgcga
      661 gatgcccatc ggcatacgcg tgatcaaggg aacgagcgaa gccgaagacg agacggtgag
      721 cctgcaaaag ccaagctatc accctgatgg tgtcattcgc ggcgagctta acctcgacca
      781 gggcctctat accgtcatca tcaccgccga agggcgcccg ccgctgcgct accagtatcc
      841 cctgcgggtg cagatgatca actatgccca gatattccgc acggcggtgg gcccgctcat
      901 cgcgctgctg cttctggccc tgttcggata caagatcatg aaatccgatc gcgtgcagcg
      961 ctggcgctcg cgccgctcat gacgcacatg ggaaccaggc gatacaggat cttgcgcgcg
     1021 ttcatcccgt atcctcttgt ccaagcaatt tttatcctca tataggcgat tctgacgatg
     1081 taccaaaaac tcctcaaacc tgcccttctt ggcctgtttt tatccatgag cctgccttat
     1141 gccatgcagg cgcacgccca tggcgggctc tcgctggccg atgacgtgtg caagctcacg
     1201 atcggcccct acaccatgca ctttaccggc taccagcccg aagccacgca ggagaaggaa
     1261 ttctgcgaag acatcccggc catcgggcgc accgtggtgg cgctggacta cattgaagaa
     1321 gccctgcgcc ccctgcccac ggaggtgcgc atcattcgcg acaccggcac ccccgatgtc
     1381 gatcctgcca agctggacga aatcaccatc ctgcacattc cccccaaggt ctacccgaac
     1441 ggctcgatca ccttcgaata caacttcccg cagccgggca aattcgtagg cctggtgacg
     1501 gtgggcgaca aacaggaata tgtctcgcgc tttcccttct cggtgggcga acccaagggc
     1561 acctcgccct tcctgctggg ggtcctggcg gctgcggccg tggctgcggt ggtattcttc
     1621 ctgcgcgggc gcaagccggc caaggtgagc cccacctgaa agctgtggcg cttgaacgag
     1681 acggggtggg gcagcgcctg gcctgacgtg cttgacatgt cagtacctgc cccagcagta
     1741 cctgaaccat actgaaacga aggagtcaaa cgaatacctg tgtcgtttgc aggggcttga
     1801 acccgggctt atccagcctc aggacttgct cgaagccatc cggatgaacc cgattaccca
     1861 gggatcggcc tggccaccca agtgcacgca ccctggagcc acacgctaat gcgcaagatg
     1921 tttgcataac gagaagaagc ggtagacagt aaccaaacga ggagcaagca atgcaatcat
     1981 ggagggtatc ggccctgatg cgggcaggca gcagttctgt cttcagtttt gtcttcactt
     2041 ccgtcttcag tcccgtcctg aaagggatcg cgatgagcgg catgctcgtc atggcgctgg
     2101 cgggatggct ctacagcgcg cctgtgagcg cacacgccat gctcaccaag gccgagcctg
     2161 cgcggcgcgc ccagctcacc cagccgcctg cgcaggtgcg tctctggttc aacgaagaag
     2221 ttgaaaagga ctacgcatcg ctcaccgtca cccacgcgga caagcccgtg accgatgcca
     2281 ggcccaggct tgccgaagat gatccccgat ccatcgtcct gcccatgccg gaactgtccc
     2341 ccgggaaata cacggtgaaa ttccgggtgt tgtcggtgga cgggcatgtg gttgactcct
     2401 cctacgactt tacggtaaag agcaaaaccc aagccaaatg atcgaggtcg tcgcaacgct
     2461 gtttcgctgg ttacagcttg cgtccaacat gatcctggtg ggcgcctgcg tattcctggc
     2521 gattgccggc tccttccatt ccccctgggt gggccgcctg cagcgcgcgc ttccctggct
     2581 gggactcatc ctgctgatcg ggctgctggg catcctgtcc accaccaccg cccaggccac
     2641 cggcatcagc gagaacgcct ggcatcctgg tgcctggctt gccctcatgc agaatacccg
     2701 catgggccat atctgggtgg ggcgcgccgc ctgtgccgcc gtagtattgg tcattgcctg
     2761 ctacctgcgc tacagcgagc ctgcgcgctg gaaatacatc ctgtgcgcca ccgccgcatc
     2821 gatcaccctg acggtaggcg cccttgccag ccactcggcg gccgaagacc tctcggtcgt
     2881 ctcgatcctg ccctacgcct tgcacatcat catggcaagc gtatggtttg gcgccttgcc
     2941 cgcctttctg gcggtgtgct tcgtctgcac cgagcctgtg aagaaagagg cgcaccccgg
     3001 gctgcaaacc gaggccatcg cgcgcatcca tgccctgttc cagtcgcgcg aacaggccat
     3061 tgccgcaggc gccacggcgc tcaagcgctt ctccaccatg gccctgccgg tcatgctgat
     3121 ggtcattgtc acgggcgtca tcatcaccga ccgcatggtc gatacgagct acagcgggct
     3181 ggtggccaca cgctacggct ggctgctcga tgccaagatt gcgcttctcg gggtcgtgct
     3241 ggtgattgcc gcgcgcgcgc gctccacctg gctgcccctg ctcgcgcaag caggcaatag
     3301 cgaaaaggcg caagccggcg gacagaagct caggaaatgg gtgagcttcg aattcctgct
     3361 ggccatgggc attgtgctgc tggccaccgt tgtggccaat accgttcctg ccaagcacgc
     3421 ctttatccag aactggccct actccttccg cttctcgctt gcggccacct ggggcgatcc
     3481 catggtcaac acccgggtat acatcggcat agccctgctc accgccgcca ttgccgccgc
     3541 cttctacggg cgcaggcgct ggagcaggaa ggtttatatc agtgccgtga tggcgctggc
     3601 caccgccgct cttgcggtgg gcctgccccc gcttgccgtg gatgcctatc cggaaaccta
     3661 ccgcaagacc ccggtgccct tcgacaccat ttcgattgcc aacggctcgg cgctctttgc
     3721 cgagaactgc gtcgcctgcc atgggccgca gggcaagggc gatggcgtca tggccaagag
     3781 ctttgccaag ccgccggtgg acatgctcac cgaaccgcat accgccaagc ataccgcagg
     3841 agacttcttc cactggctca ccttcggcat tcccgatacc ggcatgccgg tctttgccga
     3901 caagctcacc gaagaagacc gctgggatgt ggtcaattac ctgcacgcca tgtcgcgcgg
     3961 ctaccaggcg cgcctgatga gcccgaaggt caagccggag cagccccagc ccaacatggg
     4021 cccgcccaac ttctcctatg tcgcccagga tggctcaagc ggcacgctca aggacttccg
     4081 cggagaaaag aacgtgctgc tcgtcctgtt ctcctggccc caatcgcgcg agcgcctgca
     4141 ggagctcaaa aacatccagg cccagcttgc ccaggccaat accgtgctgc ttgccgtgcc
     4201 cgaagacgat cccaccccgc agcagatggc gcagctcgaa aagcttccct tcccggtggt
     4261 gacccagggg gcccacgaga tcgtgccaag ctacgccctg ttccgcagaa ccttgtccaa
     4321 gcccgatctt ctcggcgaag gcaccctgcc gcgccacatg gaattcctga tcgaccgctt
     4381 cggatacttg cgcgcgcgct ggatacccga agccgatggg cccgaatgga acactaccgg
     4441 catgctgctg caacagatcg cccagctcaa ccgcgaaaag gaaatactcc cacccccagg
     4501 ggatcatgtc cattgagcag cattgagcgg catgagcgga gaagcttgca caaggtgatc
     4561 gtagggcgcc gatgagtctg tattttcaat tccctacccc gtatttcttc aaggagagaa
     4621 aagatgcaca agttactggc cgccgtattg gtcgttaccg cgacggccgt cgcgctgccc
     4681 gccggcgcac attccgagca gcatttcgac aacatcgata cgccacacgg cggacagatg
     4741 cgcatggcgg gaccctatca cctggagctc gtgacaaagg acaaagaact cgtcctctac
     4801 gtgacggatc acggggataa caacgtgtca accgaagggg gggtcggcaa ggcaaccata
     4861 caaaagggca agtcgaaaga gaaaaccgcg atcaagctgg agcccgccgg cgaaaacatg
     4921 ctcaaaggca acggcgaatt caccctgacg cccgagacag tcgtcgtggt attcgtcaag
     4981 ctaccggacc acgaagcgca gtcagtgcgc ttcactccac tcaagccaaa gggaaaatcc
     5041 accaagaaga cccagccggc aaaaagccaa cccagcggct cgggggagca tgaccatcat
     5101 cacatgcatc attaagatcg ggcggtattg accgagccgt tgcacgacga ttcattttga
     5161 tgtcgggccg gccacacgct tccgggttgg ctatggctcc cagcttctat gtttccagcg
     5221 ctgtcgcgcc gtcagctagt gcagcatcgc gccctcggtc cacgttgacg ctactcagga
     5281 tgaataagcc gatcacgaaa tatacgcccg agatcaacat cgcaaggcga tgatcccctc
     5341 ccgaaatcca gcttacccac ccgtaggtaa ccggcccgag tatagaggat agctttaccg
     5401 cgagacccca caatccaaag aattcggccc gcctggagtt tgggctgaag tacccgacca
     5461 aggcccggcc tgctgattgc gaggcgccca gacaaaggcc ggcaacgttc gcggctgccc
     5521 agaacatcgt gcgggtttca gccatccagg cgagcagtac catgataatc caccccacca
     5581 gcgtcagcgc catcgtggga atatgtccca tcctgtcctg gagattgccg aaagcaaacg
     5641 caccggcgga ggctgtaacg ttaactacaa gtacgagaat catcgcgtcc tgggtgttga
     5701 accccatcac ttgttgggca tagattgcgg cgagtgtgat gactgtctgg atgcctgcct
     5761 gataaaagac aaggcagacc agaaaacgcg cgagatcccg gaatttaccg gcatgccgca
     5821 cagtgtccct caagcgggca aaggattcgc tcaagacgct gcggctgccc cggtggggct
     5881 gcggcagggc gcgttccttg agataaagaa aagtcggcag gctggagagc gcgaacaggg
     5941 cagcggtgat cagcatcgtg acgggaacga actgttcaga agggagtccc cgctcctgtg
     6001 cccaagtcac atatgccagc gaacagccga ggctcacgag cccgccgagg taacccaggc
     6061 tccaccccca ccccgagacc ttccccagcg cgctcccctt cgccagctcg ggcaaaaacg
     6121 cggcgatcag gttttcaccc gtaccgaaaa agaaattgcc aagtacgatg aacgtcatgg
     6181 ccagccataa atctcccggg cctgcaaaat atagcagcgc ggtgaatacc acgcacccca
     6241 cggtgctggc aaaaagcagc tgcttcttcg cgccataggc gtcagcatag gctccgagca
     6301 ccggcgcagt aaggatgatc agtccgtatg acaagcccag cgacagcgtc caggcaaacg
     6361 tgccccagct cgcattctga gcaactacgg cgacgaaata tgcgttgaat atcgccgtaa
     6421 tgacaacggt ggtatagccg gaatttgcga aatcgaacat cgcccatgcc cacacctcac
     6481 gacggctgac accggccgtc agtgaagtct ctttattcgc ggcatgcatg attcaggccg
     6541 aatatccgaa caagctgcat caaagggaaa tttccggaaa aatcagatcg gcaagctcaa
     6601 acgcccgaat agcccttacc ggggatcgtt agtggcgcga tcgccaggag ctgctgggtc
     6661 aaggggaaat gtggcgccgt tttacaagga acgcgaatca gcaaatcgat gtggacactc
     6721 aatgtttcag atgccccgga tcgccgcaga cattcacgag gacgccgatc aagtttagca
     6781 taccttccat aagcacattc acgtgctcct cggtcggctc agccgtttgc aggaaagaaa
     6841 gggtctcgtc gacggggatt tcacctttct tgatctgatg atgcgcaagg ttcaaggcaa
     6901 gcaagcgcgc gcactcggca agctgatctt tcgttgcctt ttttataagt atattggcca
     6961 gctcatatgt tccgtccaaa gctgtcgtat cgatcataga aattcctgtt cttttaagtt
     7021 actgacacct ttccttccgc ccggccccgg tattcttccc aatcgttggt ccgggggcta
     7081 ctcgccccag cgacgcaaac gagcggctac tcaacacttg cgccacccct cgaaccggct
     7141 ttggacagtg attccaatgt gctatgttaa acgcaatagc agcatttact attggactga
     7201 agtacgtatt gaatggcggg acggttaaaa ttagcgtcga tcgaacgtcg tggagcgata
     7261 ttccgaagtc atccgccccg cagagcggag gcggaacggc tcaagacacg tgcccattta
     7321 accagcttaa cgccaggagt gcccatctgg aatcccgtgc agtagctcgg tcttgaacgg
     7381 aggcggtacc aagtgctcct ttttcgacct caatgcaatg agagcagatc ctggcctgcg
     7441 ggatgcgcac ggcgcgtccc gcccacgtga atagactcac tgccagataa cttacatcag
     7501 aattacatca tattgctcct gagtataggt cccttcgact tgaagactga tcggcttggc
     7561 aatgaaatcc gccagttgcg ccaggctttg ggactcttca tcaaggagca ggtcgatcac
     7621 ctgctgtgag gcgagaatgc gaaactcgcg ggcattgaac tgccgtgatt cccgcagcag
     7681 ttcacgcagg atttcatagc acatcgtctg ggcagttctt atcttggcgc gcccctggca
     7741 ggtggggcac gtctcgcaga gtatatgcgc caggctttca cgagtgcgct tccgggtcat
     7801 ttcaaccagt cccagtgcag tgaaaccgtt caccgtcatg tgggtcctgt ctttcgccag
     7861 cgatttcttg aattccgcca gcacggcatt tttatgctcg gagtcttcca tatcgataaa
     7921 atcgatgatg atgatgcccc cgagatttcg caagcgcaac tgacgtgcta ttgcctgagc
     7981 cgcttccaaa tttgttttga atatggtctc gtcaaagctg cggacaccga cgaatccgcc
     8041 tgtattgacg tcgacggtag tcagggcttc ggtctgatcg ataatgagat aaccccccga
     8101 cttcagatcg acgcgcctgg caagtgacct ttcgatctcc tcttccaccc cgtataggtc
     8161 aaacaacggc cgttcccccg catagtggtc gatccggccc atcacttccg ccatatacac
     8221 ttccgcaaat cctttcattt tctggtaagt ctcccgggag tcgaccagga tacggacggt
     8281 atcatcgttg acaaaatccc gcagcacgcg atagctcaga ttcaaatcct ggtacagcaa
     8341 tccgggcacg gctgcagaag atttctccct gatgtcgttc catagcttgt gcagatattc
     8401 aatatctgtc tgcaattcct gctcgctcgc tgcttccgcc atagtgcgga tgatgaagcc
     8461 gcctttttcg cccgcaggca gcaagtgcct gagtttttcc cgcagcgatt ttcgctccgt
     8521 ttcatcctct attcgctgcg aaattccaat atgcgcgacc tgaggcagat agaccagcat
     8581 tcggcccgcg atgctgacct gggtcgaaag ccgcgcgcct tttgttccga tcgagtcctt
     8641 gataacctgc acaaggatgt tcttcccctc gtgcagcagc tgctcgatag gtttgtcggc
     8701 gtcttcgctc tgacgcagcc gccagatatc cgccaaatgc agaaacgcag cgcgttccag
     8761 cccgatatcg acgaatgccg actgcatccc tggcagcacg cgagtaacgc gaccgttata
     8821 tatattcccg acaatcccgc agctgctggt gcgctcgatg tggagctcct gcacggcccc
     8881 ctgttcgata accgctaccc gggtctcctg tggcgtgacg ttgacgagaa tttcgctgcc
     8941 catgagtgac gagtgaattt taagtagcag gcttaaggga gcaggactat tccagcccct
     9001 tccagcaatt gcgcagtttc gtacaaaggt aatcccataa ttccactgta gctgccggaa
     9061 atgcgcgata tgaaaacggc agccttaccc tgaatggcat aagcgcccgc cttatcataa
     9121 gcttccggcc cgctcaggta gctaacgatc tcccggtcac ccagtcttcg gaattcgacc
     9181 gtcgataccg acacccgcgt atggatatcg ccattttgag cagtgactgc tactgcggtg
     9241 aatacttggt gagatctccc cgacaacatg cgaagcatgc tctcggcatc aacggcgcct
     9301 tttggctttc cgaatacact ctgatttatt gaaacggctg tgtcagcggc aaggacagga
     9361 agatgcgccc atcctcgttc caaaactcgg gcatacccga ttccggcttt ggtacgggca
     9421 atacggcaga cataatcgcc cggaggttct tccctcaggc gggtttcatc cacgtccggt
     9481 tgccgcggcg gcgcctcgcg cagcagcaaa aggtcgaatt caacccctat ctgcttcaat
     9541 aactcgccgc gtcgggggct ccgtgaggca aggtaaacgc gcgtcgagaa atgggtcaag
     9601 gttgaggaaa tccatgaaag cggattctgc taccaaccat ggcgcatcct ctatcctcgg
     9661 tgatagggat gtcccttgat gagcgacacg gcgcggtaga gctgttccgc cagcagtatt
     9721 ctcaccagcc cgtggggcag cgtcatgttc gagagcgaga ggacttgatc cgccgagatc
     9781 ttgatttcgg catccagtcc atcggcaccc ccaatgatga acgcgatatc cctgccgttt
     9841 ctcatccatg ctccgatcgc ctctgcaagc tcaatcgtcg tcagttgctt cccgcactca
     9901 tccattgcga ttttgagaca gcgcggggtc aggacggttc taatacgaat ggcttcggca
     9961 ttcaataact gatcaatcga tttgccctcg cctcgctttt ccggtttgat ctctatcaat
    10021 tcgatacaag cttcccgcgg catgcgcctt gcgtattcgc tgaacccgtc ggccatccac
    10081 gctggcatct tgtgtccaac ggcgcaaacc aggaatttca tcgcctattc ggagtcgtct
    10141 ggccccggct tcgccacttt cctccttctt ggagcgggta gagatgcctc aggggatgcg
    10201 tcctctatct gctcaggatg atctttctgc tcggccgacg gctcgatctt ccggcgcccc
    10261 agagcggccg tactgacttt tcgtgcctga gcccataatt cttccagatt ataatgagac
    10321 cgcacagccg attgcatgat gtgcaccagc acatccccca ggtcaaccag gacccattcg
    10381 ccggcctgct cgccctctat gccatacaca gtgcccccac cggctttcac cttctctgcg
    10441 acgttgttcg caatcgcctt cgtctggcgg ttggagtcgc cgcttgcaat aatcatgcgg
    10501 tcacacaagg ttgtcatttt gcttacatca agtatttcga tatcacgcgc ttttatttct
    10561 tccagcgcgg caacgatggt tttaaccagt tttgcaagtg tcattgggtt tccagataaa
    10621 gctggtttgc taagatatag tcgagcgttg tgtcgggcag cagataacga gcgctttttc
    10681 tcaaggaaat tcgagcacgg atgttcgttg ccgatatatc cagcagggtt gtcggcgcaa
    10741 ggaaaaccag gccctggggc acgcgaatga gggcgcgggg gttcaggacc cagcggtttt
    10801 cgcattccac ccttaactcg tctggcaacg catgggtatc ttcaagcagg ggatggcccg
    10861 gacgcgcagc cacgataaag tggcaaagcc cgaacagttc acgccatttt ttccattggg
    10921 cgagcttgga gaacgcatcg gcacccagca tgaagcatag aaagacctcg tccccgagtt
    10981 cctgcttcat ttggcggaga gtgtcgacac tataagtgat tccttctcgt tgggtttcac
    11041 gcacgtccag gctaaatctt gaatttccct caatcgcaag ccggatcatc tctacccggt
    11101 gctgaatccc ggcgacaggg ggctgtcgca agcgcggaat tgcagcaggc acaaaacaca
    11161 tttcagccag ccctgccatt tcggagatct cttcggcgac ccggaggtgt ccataatgaa
    11221 tgggatcaaa agtaccgcca aaaataccgg taagcctgaa ccccacgtta accatttatc
    11281 cggcatgctc atcgattggc gcaggcttgc gcatatccgc cttcacagca atacacggcg
    11341 catgtccgcc aggacctcga caagatggga agtaaagcgg gcagcggttg cgccgtcgat
    11401 gactctgtga tcatacgaaa gggacagagg cagcatcagg cgaggaacga gggttctgtc
    11461 ccgataaacg ggtttcgtcg cggcacggga tacgcccagg attgcaactt ccggcgcgtt
    11521 gatgatcggt gtgaaccctg tgccgccaat accgccaagg ctcgaaatgg taaagcttgc
    11581 accctgcata tcggtggcct taagtttccc ttcgcgcgca agcgcggcaa ggcttgccat
    11641 ttcgcttcca atggcgatca cgcctttttg atcgacattg cgtatgaccg ggacaaccag
    11701 tccattcgga gtatcaaccg cgaatccaat atgataataa ttcttgatca ggaggttcat
    11761 atccccgtca ttgctttcag ccccgctatt gctttcaagc gaggcattga actccggaaa
    11821 ctttttcaac gccgatatgg aggcccgcat caggaaagcc aggatagtta ccttgacgcc
    11881 atccttttcc gttgtctcgt tggattcctt ccgcaacgcc tcgagttcgg tgatgtccgc
    11941 ctcgtcgaac tgcgtcacat gcggaatcat tacccagttg cggtggagat tggcggccga
    12001 aatcttcttg atgcgggaca gtggcttcac ttgcacgggg ccgaattttg cgaagtccac
    12061 ctgcggccag gacaaaaggt taagttccgt cccggcaccc cgtggcttag ccagctcagc
    12121 cttgacaaag gcctgaacgt cttcctggag tatgcggctt ttcggtcccg tacctttcac
    12181 caaaccgata tcgaccccga gctcccgcgc aaattttcgg acggaagggc tcgcatgagc
    12241 ggtcgaaaaa gcgcgctcgc tggaaggaga agaaggcagg ggtaccggtg ggggcttggg
    12301 tgcacgagcg ggcggaggag gcgttgcgcg actgatctcg gccctggctt cccccccgcg
    12361 aggtgcggtg tcggaaccag agggctgcga cggagcaggc tgcggggtcg aagccggtgc
    12421 cgctgccgct gccgatgtgg agccaggctg tggaaccggg gcggccttgt tttcgtctcc
    12481 cacttccatc cgaagtatag gcgtgccttc tgaaacctta tcccccggtt ttatagaaat
    12541 ttccttgacg gtgccggtga agggggaggg tacctctatt
//
LOCUS       DASSHJ010000222         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1232373,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000222 DASSHJ010000000
VERSION     DASSHJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232373"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFI43_08695"
     CDS             <1..199
                     /locus_tag="VFI43_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062240.1"
                     /translation="GIDPGLLDSRTAEELGYLARRPAYSAMSSKRGVLLPALDNAIDR
                     YIHIKRDEMVLAYPKRKAGAS"
     gene            705..995
                     /locus_tag="VFI43_08700"
     CDS             705..995
                     /locus_tag="VFI43_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062241.1"
                     /translation="MKGLLSVSSGSKKVWAGAVAVLIFGLGGAAYAAGGTSGSDMSRS
                     SGDGEGSGRTMGGAPKGKEPSLGSQRPEDPIRVPVQQARLRERQQEKRDIAI"
     gene            1094..2299
                     /locus_tag="VFI43_08705"
     CDS             1094..2299
                     /locus_tag="VFI43_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062242.1"
                     /translation="MALNLFDDKGCPLDKQQFTWKEMVQTPISKLDDDAFTRVRVILM
                     NGQEMEALRFSHSCARMNDQYRLPLAKVRRIEQHQATMVNGLIGADHSTLETTIGYEQ
                     VAIEVTAAVAQAEPDEYLAQVYRFGMLEDFDHLYRYSAMLDRLEGKDANNITQCYTDI
                     VPGRPTVIEHRAPQDDLRNHYDRTKAQPISKLHALTIMAAEQQTQNFYLNVGPMYADP
                     VARQLYTEIASIEEQHVTQYESIIDPNETWLEKWMLHEANEVYNYYGCVQQESNPRIK
                     AIWERCLDYELGHLQVAMEMFKQHERRDPAEILPASLPEPIKFESQREFVRKVLHQEV
                     DLRARGTQFVNKSQEGPESKQYREHLNSSGSPTNTVAAGYEWTPGTELVRGAGVSYTE
                     NAKDKEVTH"
     gene            2327..3061
                     /locus_tag="VFI43_08710"
     CDS             2327..3061
                     /locus_tag="VFI43_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7062243.1"
                     /translation="MEKSTALGKNRTGIDMSPIDAKEMVALSNSTPPSSPGDARDIAK
                     MRGEYISEHDTLGSIPPPGTLKGVASTAMKKLTGKNPEAFIDKLGCRLAFERTGVRLY
                     EALIAKCSAGAVGASKVSLDTLWEFRNQELQHFKLVESAIRSIGADPTAQTPSADVDG
                     MIALGIVQVLTEPRTSVEQCLDAMLTVELTDHAAWELLIEFATKMGMEDMARDFQGAL
                     QQEENHLRQVRQWFRDLTLSDGGVES"
     gene            3400..>3677
                     /locus_tag="VFI43_08715"
     CDS             3400..>3677
                     /locus_tag="VFI43_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062244.1"
                     /translation="MDELKNEIFYDELAIWGGLECTINRVQDTYFSQLERNGHARRLS
                     DIDSFASLGIRAIRYPVLWEHVAPNGIDEADWSWPDERLPALKERQVAP"
BASE COUNT          866 a          966 c         1071 g          774 t
ORIGIN      
        1 gggtattgat cccggcctgc tcgattcgcg caccgctgag gagttgggat accttgccag
       61 gcgtcccgcc tacagcgcga tgtccagcaa gaggggcgtt ctccttcccg ccctggataa
      121 cgccatcgac cgctatatcc atatcaaacg cgatgaaatg gtacttgcgt atccgaagcg
      181 gaaagcgggg gcgagctgag agacaagcag tatccccttc aggtttttgt cataagggca
      241 gttgcatgac gcgctgcccg ttttttttgc ttcacgtact tcctgtccct tcgctacacc
      301 ttttgtttca catctgatct tgccgggccg ggtattcccg tcattgtaac ctgaggccgc
      361 cagcctgcgc cgcctcccgt tttgtctgcc tgccccgctg gcttctaccc cccaaagccg
      421 accctacttc atcatccttg cttgcttgct tattaataca acagcatatt tctgctggat
      481 tttctagcgc cctgtgtgtg ttcgcgaaca gaggcatcaa ggcaccttct ttattattat
      541 tgtcattcgg tgactcatcc atattccgcg aggttcccca atgaatgaca tcagcatggg
      601 atgcatttta atgttaacgg catttttaca cactctgttg catatagatg tcgaggcatg
      661 aggaaccgct cgctactgtg atcaacgggc tgacaggagg aaaaatgaaa ggcttactca
      721 gtgtatcaag tggaagcaag aaggtttggg ctggcgcagt tgcggtgttg attttcggat
      781 tgggcggcgc tgcttatgcg gcaggtggca cttccggcag tgatatgtcg cggagtagtg
      841 gagacggaga gggttctggc cgaactatgg gtggtgcgcc caaaggaaag gagccgagcc
      901 tgggcagcca gcggcctgaa gacccgatac gcgttccggt tcagcaggca aggcttcggg
      961 agcgtcaaca ggagaaaagg gatatagcaa tctgagaggg gataaaggcg gaaagggcgc
     1021 gggcaatacg acgacaacga acgctgtccc tgcgaaggac taaaccagcg agagactaaa
     1081 ccaggaggat acaatggcac ttaacctgtt tgatgataaa ggctgtccgc ttgacaagca
     1141 gcagtttacc tggaaagaga tggtgcaaac accgatcagc aagctggatg atgacgcgtt
     1201 tacgcgcgtt cgggtgatcc tgatgaacgg acaggaaatg gaggcgttgc gcttctcgca
     1261 ttcgtgcgcc cgcatgaacg atcaatatcg gctgccgctg gcaaaggttc ggcgtatcga
     1321 gcagcatcag gctaccatgg tgaacggctt gatcggcgca gaccactcca ctctggaaac
     1381 caccataggc tatgagcagg tcgcgatcga ggtgaccgcg gctgtggccc aagcggaacc
     1441 cgatgagtac ctggcccagg tttaccggtt tgggatgctt gaggactttg accacctcta
     1501 ccgctatagc gcgatgctgg accgcctcga gggaaaagat gcgaacaaca tcactcaatg
     1561 ctacaccgac atcgttccgg gccgtccgac ggttatcgag catcgggcgc cccaggacga
     1621 cctgcgcaac cactacgatc gcacgaaggc ccagccgatc tccaagctac atgccctgac
     1681 gatcatggcg gccgaacagc aaacgcaaaa tttctatctg aacgtggggc cgatgtatgc
     1741 cgacccagtc gcgcgccagc tctataccga gattgcctcc atagaagagc agcatgtcac
     1801 gcagtatgag tcaatcatcg accccaacga gacgtggctg gagaagtgga tgttgcacga
     1861 ggccaacgag gtttacaact actacggatg cgtgcagcag gaatccaacc cccggatcaa
     1921 ggcgatctgg gagcgctgtc tcgactacga gctggggcac ttgcaggtgg cgatggaaat
     1981 gttcaagcag catgagcgac gcgatcctgc cgagatcttg ccggcaagtt tgcctgagcc
     2041 gatcaagttt gaaagccagc gcgaattcgt gcgcaaggtg ctgcaccagg aagtggattt
     2101 gcgcgcccgc ggcacgcagt ttgtgaacaa atcacaggaa gggccggagt ccaagcaata
     2161 tcgcgagcat ctgaactcgt ccgggtcccc aacgaatacc gtcgctgccg gttatgaatg
     2221 gacgcccgga accgaactgg tgcgcggcgc cggggtttcc tacacggaga acgccaagga
     2281 taaggaagtc acccattaat ccgccgaaga tttcaggaga cagacaatgg aaaaaagcac
     2341 ggctttagga aagaatcgta cggggataga catgtccccg atagacgcga aggaaatggt
     2401 ggcgctgtca aattccaccc ccccttcatc cccgggagat gcgcgcgaca ttgcaaaaat
     2461 gcgcggcgag tatatctccg aacatgacac tctcgggtcc atcccgccgc cgggcactct
     2521 gaaaggagtg gcgagcacgg caatgaaaaa gctgaccggc aagaacccgg aggcattcat
     2581 cgacaagctc ggatgccggc tcgcctttga acgaactggt gtgcggctgt atgaggcgct
     2641 tatcgcaaaa tgctcggccg gcgcggtggg ggcgtcaaag gtttcgctgg atacgttatg
     2701 ggagtttcgc aaccaggagt tgcagcactt caaactggtg gaaagtgcaa ttcgctcgat
     2761 cggggccgat ccgacggcac agacgccctc tgccgacgtt gacggcatga ttgcgctggg
     2821 gatcgtacag gtactcaccg aaccgcgcac gtcggtagaa caatgcctcg acgccatgct
     2881 gactgtagag ctcaccgatc atgctgcatg ggaactgctc atcgaattcg cgaccaagat
     2941 gggcatggaa gatatggccc gggatttcca gggagcactg cagcaagagg agaaccattt
     3001 acggcaggtt cggcaatggt tccgggattt gacgttgagc gacggcggcg ttgaatcgta
     3061 aatcgtgttt tgagcagagc ccggcacgca agatactcgc gtgccggaaa agtcctctgc
     3121 cgtcagattt caaataattt tccactaaaa gactgcgggc gaaagaaacg cgagtttgta
     3181 tatagccgcc cgaggaaggt tttgcttcca gccggttaat cctttctctt tttcggtttt
     3241 tgccttcagc ttccagttca acttatacaa aaataacacc gtactagtcg ccattgcgac
     3301 tcgcaatgga ttcgtcgata aattgatcgc gcctgctcgc ctctgaatga gcgaggttcg
     3361 gacagtgccg cctccaattt tatcaatcga gggtcgtgta tggatgaatt gaagaatgag
     3421 attttttatg acgagcttgc aatatggggt gggctggaat gcacgatcaa tcgggtgcag
     3481 gacacgtatt tcagccaact ggagcgcaat ggacatgcga ggcggctaag cgatattgac
     3541 agctttgcct cgctggggat ccgcgcgatc cgttacccgg tgttgtggga acatgtggct
     3601 ccgaatggaa tcgatgaggc ggattggtct tggccggacg agcgcctgcc cgcgctgaaa
     3661 gaacgccagg tggcccc
//
LOCUS       DASSHJ010000223         3942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1451404,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000223 DASSHJ010000000
VERSION     DASSHJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451404"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFI43_08720"
     CDS             complement(<1..340)
                     /locus_tag="VFI43_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7062245.1"
                     /translation="MTDCIERYDSTRAQTLKLVESLTPEDHMVQSMPEASPAKWHIAH
                     TTWFFETFILVPHVPGYVPFDERFGFLFNSYYKQLGNHPYRGVRGTFSRPSLDEIYLY
                     RSHVDEAIHKL"
     gene            770..1324
                     /locus_tag="VFI43_08725"
     CDS             770..1324
                     /locus_tag="VFI43_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7062246.1"
                     /translation="MSYEDRDTYGMYKSYDNEGPGPRLMGAGTLMGEDVYNTKDEDLG
                     DIKEIMLDMNTGRIAYAVLSFGGFLGIADKLFAVPWSALKLDTENKRFILNVDKDRLE
                     SAPGFDKDHWPDMADPTWQNTINSYYGTTSYTDTGYTGSSQTGTSSTKDYTTPAHRNN
                     ERIIPVAPVGTDHVSDKLDDFTHK"
     gene            complement(1332..3707)
                     /locus_tag="VFI43_08730"
     CDS             complement(1332..3707)
                     /locus_tag="VFI43_08730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7062247.1"
                     /translation="MKYEVSVRSLCEFTARKGDLDMRFSGGPSAIEGIAGHGRVTSRR
                     PPHYEREITLIGEYRHLVVRGRADGFDHVENRLEEIKTYRGDLASMPRNQREFHWAQL
                     RIYGHMLCEKLGLPVIHLTLVYFDIASESETLLDETADATGLRRYFEQHCEYFLCWSE
                     QELGHRHARDESLAALRFPHSSFREGQRQLAETVYRTARQGASLMAQAPTGIGKTIGT
                     LFPFLKACPSRKLDKVFFLTAKTSGRQPALDALALMHKGEQRLNLRVLELISRDKACE
                     HPDKLCHGESCPLARGFYDRLPAARAAALAICELESGEASSNTLSRNTLRAVALDHGI
                     CPYYLSQELIRWSDVVVGDYNHYFDLNATLHELTVANQWRVGVLVDEAHNLLERARKM
                     YTAELNPAILCVAYTAAPSKLRKPLGRLQRCWNKFGKGQDGHYSVYSAIPDQLTNLLQ
                     RAIAAVIEHAVEDPNGLTGALRDFYFEALHFRRISELFDKHWLFDSTKAGRSPGKGDL
                     SFSLPENVLCLRNIVPAPFLAQRFASAQASVLFSATLAPPRFYRDMLGLSPDTTWIDV
                     PSPFKAEQLSVRIVSHISTRFQERDASISPIVDLISRQYKTRPANYLSFFSSYDYLRQ
                     VVTLLRSRYPEIPVKEQTRGMEETEQAEFLAGFTSASQHIGFVVLGGSFAEAIDLPGK
                     RLVGAFIATLGLPQVNGVNEQMRQRMETLFGAGYEYAYFFPGIQKVVQAAGRVIRTQQ
                     DEGVIYLIDDRYARMDVRQLLPTWWEVKQLKKERQAHLASPVFTRVAGPGM"
BASE COUNT          925 a         1065 c         1071 g          881 t
ORIGIN      
        1 aaagcttatg tatcgcctcg tctacgtgag acctgtataa ataaatctca tcgagggacg
       61 ggcgcgaaaa tgtgccccgc acgcccctat aaggatgatt acccagttgt ttgtaatatg
      121 aattgaaaag gaaaccaaac cgttcgtcga atggaacata gcccggcacg tgcggtacga
      181 gaataaatgt ctcgaagaac caggtggtat gggcaatatg ccattttgcc ggactcgcct
      241 caggcatgga ttggaccatg tgatcttccg gcgtaagcga ctcgacgagc ttcaaggttt
      301 gggcgcgtgt gctgtcatat cgctcgatgc aatccgtcat caatggggct cgactggacg
      361 gaaggcgaga tatacgtaga attccgttcc tttgcatcct tatcctttta ttaacgctgg
      421 catctcgcca gtatctttgt ccgtaagtga ggtctcgctc ggcaggtgca agcgcggctg
      481 cgagggaaaa ttgcaatgcc atgtcggtct cgtggtcata cgaacccgat tttctaacgg
      541 gcgcggccga gcttccgtgc gcttgcagac ataagcctat tcagtcccct gatcgttcct
      601 taagcaaagc cgttctttgt acgttaccga accgacctct gccgtcgaga ggcgcactat
      661 gggcatgtct cggcgcgtta tccctcatcc ttgggggcgg ccgaagggcg gaacaaaagg
      721 atcgaaaagg aagtttgacg tttaaaaatt actttgacag gagatgacca tgagctatga
      781 agatcgcgat acctatggca tgtacaagag ctacgataat gaagggccgg gaccccggct
      841 gatgggcgcg ggtaccctca tgggagagga tgtctacaac accaaggacg aagacctggg
      901 tgacatcaaa gaaatcatgc ttgacatgaa caccggacga atcgcatatg ccgtgctgtc
      961 ctttggtgga tttctcggta ttgccgacaa gctttttgcc gttccctgga gcgccctgaa
     1021 gctggatacg gagaacaaac ggttcatact caatgtcgac aaggaccgcc tggagtccgc
     1081 tccggggttc gacaaggatc actggcccga tatggcggat cccacctggc agaatacgat
     1141 aaattcgtat tacggaacga cgagctatac cgataccggc tacacagggt cgagccaaac
     1201 aggaaccagc tcaactaaag attacacgac gcctgctcat aggaacaacg agcgtatcat
     1261 tcccgtcgca ccggtcggca ctgaccatgt ctcggacaag ctggacgatt tcactcacaa
     1321 gtaagatgta atcacatgcc cggccctgcc acacgggtaa aaacaggaga ggcaagatga
     1381 gcttgcctct ctttttttaa ctgttttacc tcccaccacg ttggcaggag ctggcgaacg
     1441 tccatccgtg cataccggtc atcaataagg tagatcactc cctcatcctg ctgagtgcgg
     1501 ataacccgtc ctgcggcctg caccaccttc tgaatgccgg ggaaaaagta cgcatactcg
     1561 tagccggcgc caaacaacgt ctccatacgc tgccgcatct gttcatttac cccgttgacc
     1621 tggggcagtc cgagcgtcgc aataaaggcg cctaccaagc gttttccggg gaggtcgatg
     1681 gcctcggcaa acgatccgcc cagaaccaca aagccgatgt gctggctggc tgaagtgaaa
     1741 ccggccaaaa actccgcctg ctccgtctct tccatgccgc gtgtttgctc tttaacggga
     1801 atttcgggat atctcgatct gagcagggtc actacctgtc gcaggtagtc gtagctgctg
     1861 aagaacgaga ggtagttggc cggcctggtt ttatactggc gggaaatcag atcgacaata
     1921 ggggaaatcg aagcatcacg ttcctggaag cgagtggaga tgtggctgac gatccgtacc
     1981 gacaattgct cggctttgaa cggtgatggc acatcgatcc acgtggtatc aggcgaaagg
     2041 ccgagcatat cccggtaaaa acgcggcgga gccagtgtcg ccgagaacag gacgcttgcc
     2101 tgcgcgctcg cgaagcgctg ggcaagaaag ggagcgggaa cgatattcct gagacaaagc
     2161 acgttttccg gcagggaaaa tgacagatcg ccttttccgg gacttcttcc agccttggtc
     2221 gaatcgaata gccaatgctt gtcgaataat tcgctgatcc gccgaaaatg cagcgcttcg
     2281 aagtaaaaat cccgcaatgc gcctgtcaag ccattcgggt cttccacagc atgctctatg
     2341 accgctgcaa tggcgcgctg caacaggttc gtcaattgat caggtatagc cgaataaaca
     2401 gaataatgtc cgtcctgacc tttgccgaat ttattccagc agcgctgcaa gcgtcccagc
     2461 ggcttcctca gtttcgatgg ggcggctgtg tatgcgacgc agagaatggc tggattcagc
     2521 tcggccgtat acatttttcg cgcacgctcg agaaggttat gggcttcatc gaccagcacg
     2581 ccgacgcgcc attggttggc aaccgtcagt tcatgcaatg ttgcgttcag atcgaagtaa
     2641 tggttgtaat cgcctacaac cacgtcagac caccgtatta actcctgcga gagatagtag
     2701 gggcagatgc cgtggtcaag ggcaacagcg cgtagagtgt ttctactgag ggtgttagag
     2761 ctcgcctcgc ccgattcgag ctcgcatatt gcaagtgccg cagcccttgc ggctgggagg
     2821 cgatcgtaaa acccgcgggc gagtgggcag gactctccgt ggcatagctt gtccggatgt
     2881 tcgcacgcct tgtcgcggga aatcagctca agcactctca ggtttagcct ctgctcgcct
     2941 ttatgcatca gcgccagggc gtccagcgca ggctgacgtc ccgacgtttt ggcggtcaga
     3001 aaaaaaacct tatccagctt ccgactgggg caagctttaa ggaaggggaa aagcgtaccg
     3061 atggttttgc caatgccggt gggagcttgc gccattagcg aagcgccttg acgcgctgtc
     3121 ctgtataccg tctcagccaa ttgcctctgg ccttcacgaa aggacgaatg gggaaaacgc
     3181 agcgcagcca aggattcgtc gcgggcatga cggtgcccaa gctcctgctc agaccagcac
     3241 agaaaatatt cgcaatgctg ctcgaagtaa cgcctgagcc cagtcgcatc agcggtctcg
     3301 tctagcaaag tttcgctctc gcttgcgatg tcgaaataca ccagtgtgag atgtatgacg
     3361 ggtaacccca gcttctcgca cagcatatgg ccgtagatcc tgagttgcgc ccagtggaac
     3421 tcacgctgat tgcggggcat tgaggccaga tcgccgcggt aggttttgat ttcttcgagc
     3481 cgattctcaa cgtgatcgaa gccatccgcc cgcccccgta caacgagatg ccggtattca
     3541 cctatcaggg ttatttcgcg ctcgtagtgc gggggccgcc gggacgtgac cctgccatgc
     3601 ccggcgattc cttcaatggc gctcgggcct ccactgaagc gcatatcgag atcacctttc
     3661 ctcgcggtga attcgcacag ggaacgcaca gatacttcgt atttcattcg gatttcaagc
     3721 cggtttggtc attttacgga tcgttgccta tccacctatg ctgtgattca ttttcacaag
     3781 aagcaaacgg catgatgtga tggctctcat tctccagata tcttggcagg ttatccccaa
     3841 gatatctcat gaacgtcaac cgcacgcctt gttcgccact tgctaccgag ccattcatcc
     3901 ttgacagccc gaaatgggtg acataggttg attagagcaa ct
//
LOCUS       DASSHJ010000224         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1375806,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000224 DASSHJ010000000
VERSION     DASSHJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375806"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFI43_08735"
     CDS             complement(<1..724)
                     /locus_tag="VFI43_08735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7062248.1"
                     /translation="MDFYNNKTDEEKARWYSAGATFYAEGRPTYPVALTTAVAAVAGW
                     REDSRILEIGSGPGTATQGFVGFGGEILCVEPNSEFVSLAQQAMSHFSNVAFQMSTSE
                     EAQLAPPYDIILAASSFHWIDAGRGLKRMRDVLSPMGFVVLLWNKEPQPITTQADAVD
                     EAFRRSGFTQQASRQSREETAKVFWDLSQPLQGAAFIERLYSHTLTSDDYSIDRFIAL
                     QRSLSPFLALPLQEQQKVAGKLT"
     gene            complement(1080..2213)
                     /gene="holB"
                     /locus_tag="VFI43_08740"
     CDS             complement(1080..2213)
                     /gene="holB"
                     /locus_tag="VFI43_08740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7062249.1"
                     /translation="MNDIYPWQQAIWQKVASTRRPHASIHDPSISHALLLKGRKGLGK
                     LSFASYLAKARLCRNPTKEEEACDSCASCRWFEQGAHPDFRLVQPEALPASSAVAASP
                     GDTSSEGDRDDGSEGSAEATPGPEAEPGNPKSRKKPSKQISVEQIRALADFINISSHQ
                     GGYKIVVIHPAETMNLAAANALLKNLEEPPPLTLFILVAHQSQHLLATIRSRCVQISM
                     PVPEPSVSIDWLRARGVKNAELCLASAGYAPLSALEFDDETYHERHLAFIKHISVPGR
                     LNPISLVEGLQKTDLPTIVNWLQKWCYDLLSFRIAGKIRYHHDQFVAIEALAPTLNPQ
                     ALVTYLRTLTKIQQLASHPLNERLFLEEMLFSYILALNDSHST"
     gene            complement(2210..2830)
                     /gene="tmk"
                     /locus_tag="VFI43_08745"
     CDS             complement(2210..2830)
                     /gene="tmk"
                     /locus_tag="VFI43_08745"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7062250.1"
                     /translation="MPRGKFITLEGIDGAGKSTQIARLADLLRERGKTVVVTREPGGT
                     SLGERLRELLLDNCQTMHPETEALLMFAARREHLDKVILPALEQGNWVICDRFTDASF
                     AYQGGGRGLPAEKLAELERWVQGNVQPDLTLYFDITSELGRLRTSGVKNPDRFEREHD
                     EFFRRVRDGYLDRAYRFPERILVVDAGRSLEEVNRTVEDIAISYCK"
BASE COUNT          646 a          805 c          776 g          717 t
ORIGIN      
        1 ccgtcaattt ccccgccact ttttgctgct cctgcagcgg taatgctaaa aaaggggaga
       61 ggcttcgctg aagtgcaata aaacggtcga tgctatagtc gtcagaggtc agagtgtgac
      121 tgtacagcct ttcgatgaaa gcagcacctt ggagcggctg cgacaagtcc cagaagactt
      181 tcgccgtctc ttcgcgggat tgacgggagg cttgctgcgt gaaacccgac cgcctgaagg
      241 cctcgtccac tgcatccgct tgcgttgtaa ttggctgagg ctccttattc cagagaagca
      301 ccacaaaccc catgggagaa agcacatctc tcatgcgctt cagtcctcgg ccggcgtcaa
      361 tccaatgaaa ggatgatgcc gccaggataa tgtcatatgg tggagcaagc tgtgcctcct
      421 cggacgtgct catttgaaag gccacatttg aaaaatggct catggcttgc tgggcaaggg
      481 aaacaaactc actgtttggc tctacacaaa gaatttcgcc accgaagccg acaaatccct
      541 gggtggccgt gcctgggcct gaccctattt cgagtatcct cgagtcttca cgccagccag
      601 caaccgccgc tacagcggtg gtcagggcga ctggatacgt gggacgacct tccgcgtaaa
      661 acgttgcccc agccgaatac cagcgagcct tttcctcgtc agtcttgtta ttgtagaagt
      721 ccatcccctc acctcatctc aaaacgaccg aatagcgaat tgtccgctct tggcgcgttc
      781 gtgtcatgcg gggaaccaaa cttccatgcg cttcaaccga tcagggttcc tcccaccgta
      841 tatctatgcc cctttaatgt tcaggggccg aatagctttt cagtgtggcg aacgagcgaa
      901 gtgcccggtt cgcaggacgg cgcaggctcg cttggggcag ttgcgtattt tgatggcatg
      961 gcagcggaaa tagttaaccc ggaagtagtc tcatcaacca gcgcgtccat caaggatttt
     1021 ttactttgct gctttcgagg atggacaaat tcttaacgta cttcagcttc gatgcgtatt
     1081 tacgtggaat gagagtcgtt cagcgctagt atgtaggaaa acagcatctc ctcgagaaac
     1141 aggcgttcat taaggggatg gcttgcaagc tgctggattt tggtcaaagt ccgcagatac
     1201 gtgaccaaag cctgcggatt cagagttggc gccagcgcct cgatcgcaac gaactggtcg
     1261 tgatgatagc gaattttgcc ggctattcga aagctcaata aatcatagca ccacttctgg
     1321 agccagttaa ctatggtagg cagatccgtt ttctgcaatc cctccacaag agaaatggga
     1381 ttcagtctac ctgggacgct gatgtgtttt atgaaagcca gatgccgttc atgatacgtt
     1441 tcgtcgtcga attcgagcgc tgacaggggc gcatacccgg ccgaagcaag gcacagctcc
     1501 gcatttttga cgcccctcgc gcgcagccag tctattgaga cagagggttc aggcacgggc
     1561 atggagattt gcacgcagcg gctgcgtatg gttgcaagca ggtgctgcga ctgatgcgcg
     1621 accaatatga acaaggtgag tggaggcggc tcctccaggt ttttcaaaag cgcattggcc
     1681 gcggccaaat tcattgtttc cgccggatgg atcacgacga ttttgtagcc gccctggtga
     1741 ctggaaatat tgatgaaatc cgcaagggct ctgatctgct cgacgctaat ctgcttgctg
     1801 ggttttttcc ttgactttgg gtttcccggt tctgcctcgg gacctggtgt tgcctcggcc
     1861 gaaccctcac tcccgtcatc cctgtcacct tcgctgcttg tatcgcctgg cgaggcagcg
     1921 actgcagaag acgctggcag ggcttcaggc tgaacgagac ggaaatcggg gtgtgcccct
     1981 tgctcaaacc atctgcaact cgcgcaactg tcgcaggctt cctcttcttt ggtgggattt
     2041 cggcacagtc ttgctttcgc aagataactt gcaaaagaca gtttacccag gcctttcctg
     2101 cctttaagca agagcgcatg gctgatggaa ggatcgtgta ttgaagcgtg aggacgcctc
     2161 gtggaagcaa ctttctgcca tatcgcctgt tgccacgggt aaatatcatt catttacagt
     2221 atgatatggc aatatcttca actgttctat taacttcttc cagcgaacgg cccgcatcca
     2281 ccacgagtat ccgctcggga aagcgatatg ctcggtcgag ataaccatct ctcactcttc
     2341 gaaaaaactc atcgtgctct cgttcgaacc tgtccgggtt tttgacgccg cttgtccgca
     2401 atctaccaag ttcggatgtt atatcgaaat aaagtgtcag gtcgggctga acatttcctt
     2461 gtacccaacg ctcgagttcg gccagtttct cagccggtaa tccccttccg cctccctggt
     2521 atgcaaagct ggcatcggtg aagcggtcgc aaatgaccca gttgccttgc tccagcgcgg
     2581 gcaggatgac cttatccaga tgctcgcggc gcgctgcaaa catcagcagt gcctctgtct
     2641 cggggtgcat cgtctggcag ttgtccagca ggagttctcg cagcctttct ccaagggagg
     2701 tacctcccgg ttcacgcgtg acgacaaccg tctttcccct ctcccgcagc agatccgcga
     2761 gccgggcgat ctgggtactc ttgcctgcgc cgtctatgcc ctccagggtg ataaatttcc
     2821 ccctgggtat ctcgcaaacg gtcatgtccg aaattcgatc aagcgacgct aaagttggaa
     2881 aaagcactgc ttgaggtagt gttgcctccc acacagttta gcttatactg gtgaatggca
     2941 acag
//
LOCUS       DASSHJ010000225         7704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1350325,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000225 DASSHJ010000000
VERSION     DASSHJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350325"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VFI43_08750"
     CDS             complement(<1..1281)
                     /locus_tag="VFI43_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HET7062251.1"
                     /translation="MEELSTQLLVYLKGIWKYRWIAVIAAWIVAVGGWVIVYKLPDDY
                     QASARIYVDTQNVLKPLMSGMTVSPDLQQQVSIMSRTLISRPNVERVIRMVDLDIKAK
                     DVKDQEKQVKELMEKIQLATTGRDNLFTISYSNRSPKLAKDVVQSLLTIFVEGGLGDK
                     HDSSSAIRFLDEQIQSYEEKLIAGENNLKAFKQKNIGLMPQQGSDYYSQLSQAEEEIN
                     KARLELREAEQARDAIKRQITGDEPVLLVDQDESSSIAGNTELDSRIAELNKNLDILM
                     LNYTELHPDIISTKRLIAQLEERKREEAKLIRPSGGDPGKNYSPMLQQLNVALAEAEA
                     DVASMKARVDEYTNRYNRLRSLSNAVPQVEAELAQLNRDYQVNKSNYEKLLERRESAK
                     ISGQLGSATDLMSFRIIDPPTVPDLPSGPNRTRLY"
     gene            complement(1417..2031)
                     /locus_tag="VFI43_08755"
     CDS             complement(1417..2031)
                     /locus_tag="VFI43_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HET7062252.1"
                     /translation="MLHIFKWLALLATVFPILSLGGCKSYPTLSSSEKYLSHDYIIGP
                     GDNVDVIVWRNPEVSMAVPVRPDGKITTPLVEDLQASGKTSTQLARDIEQALSKYIQQ
                     PVVTVVVTEFVGPYTEQIRVVGEATKPQALPYREEMTLMDVLIAVGGMTDFAAGNKAR
                     IIRVVDGKQQQFSVRLDDLIRDGDISANVPMRQGDVLVIPESFF"
     gene            complement(2428..3423)
                     /gene="queG"
                     /locus_tag="VFI43_08760"
     CDS             complement(2428..3423)
                     /gene="queG"
                     /locus_tag="VFI43_08760"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET7062253.1"
                     /translation="MRVAHAKADMTESEHGLQQWLSKGYQGEMDYMGKHGTRRTRPDE
                     LVPGTVRVISVRMNYMPGAADSWKVIQNGNKAFISRYAHGRDYHKVLRARLQKLAERI
                     SAEVEHFNYRVFTDSAPVMEVEWAQKAGTGWRGKHTLLLSRNAGSMFFLGEIYTDLPL
                     PVDDPVGNYCGSCTKCIDICPTGAILAPYRLDARRCISYLTIEHKGSIPEPLRPLIGN
                     RVYGCDDCQLACPWNKFAVKTEEEDFAVRNGLDDVSLIELFGWSKEEFDTRLAGSPIR
                     RIGYEQWLRNLAIGLGNAPASSDVAKVLEARRDDASALVREHVQWALQSQDKRGV"
     gene            3560..4033
                     /gene="tsaE"
                     /locus_tag="VFI43_08765"
     CDS             3560..4033
                     /gene="tsaE"
                     /locus_tag="VFI43_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7062254.1"
                     /translation="MFHPRDYLALCLADEFATIELGKKIAAIIRPGLIIFLTGNLGAG
                     KTTLVRGILRGLGYVEKVKSPTYNLIEVYKISRLYLYHFDFYRFNDPFEYEEAGFREY
                     FNSESICFVEWPERAAGLLPAADLSLFFEISGTKRVVKIYPGTEAGNGCLEYLSH"
     gene            4128..5387
                     /locus_tag="VFI43_08770"
     CDS             4128..5387
                     /locus_tag="VFI43_08770"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7062255.1"
                     /translation="MFLAFENLLFAAPIPQSISISSARVWPAPEYTRLTLEASLPIQY
                     SLNTVKNPHRIVIDLEHVLLTGEIEKLGERIDSSDPYVRSIRVGRYKPGILRLVVDLK
                     TEAAPQAFILKPVGNYKHRLVLDIYPVVPPDPLMAFLSENITAVLQDDAASLKTGNKA
                     AMNDDSRSGTVSSKKAKPQTARLITVAIDPGHGGEDPGAISRSGTNEKDITLAIARKL
                     KARIDKEPNMRAALTRDGDYFISLPMRLVKARQWNADLFVSVHADAFIKPHARGSSVF
                     ALSERGATSAAARWLAKKENEADLIGGVNLDIKDPYLKQTLLDLSQTATISDSLKLGK
                     EVLSEIGDINHLHKNDVEQAGFAVLKSPDIPSILVETAFISNPDEEKKLKDAAYQERL
                     AAAIVVGIKRYFANNPPAARSKMVSLD"
     gene            complement(5436..5975)
                     /locus_tag="VFI43_08775"
     CDS             complement(5436..5975)
                     /locus_tag="VFI43_08775"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7062256.1"
                     /translation="MRYCSNCGTTVELKIPEGDKLLRHVCTACGVIHYQNPKIVVGCI
                     PEWEKKILLCRRAIEPRPGLWTLPGGFMENAETLAQGAARETLEEANARVQMGDLFAI
                     YNLPHINQVHVFFRAQLLDLDFEPGIESLDVRLFEEEEMPWDQLAFRVIDEPLKRYFQ
                     ERRQGKLGFHIATIDEIRK"
     gene            6129..>7704
                     /locus_tag="VFI43_08780"
     CDS             6129..>7704
                     /locus_tag="VFI43_08780"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7062257.1"
                     /translation="MKIAIAQINCVVGDFSANIRKILDYCNRARQQEASLVVTPELAL
                     CGYPAEDLLLRPGFVNACAEALADLSRQVEGITLLVGHPYCSGGKLHNSASVISEKRI
                     IATYLKNLLPEESVFDELRYFQPGLDPCVFELDGRKFGVNICQDIWEQGTAIRAKNAG
                     ADFLLVLNSSPYHFRKQDLRHRIVRERISETGMPVVYANLVGGQDELVFDGASFAMNE
                     KGEVTHQFDAFEEVLGLIELQDNQLVQGQVSASGSVEESTYRALCLGIKDYIGKNRIP
                     GVLLGLSGGVDSALTLALAVDALGPDKVEAVMMPSQFTTDISLADARAIAEALGVRYR
                     ELPIDSIFDRFLGTLASAFETQSDQKSPDLTAENLQARVRAALLMALSNKTGSIVLTT
                     GNKSETAVGYCTLYGDMAGGFAVLKDVTKTMVYRLCHYRNSLSRVIPERVITRAPSAE
                     LRPNQTDQDSLPPYALLDAILEAYVEHDLSLTEILRMNYPEADVRRVVGLIRQNEYKR
                     HQSPPGIRVTSRGFGKD"
BASE COUNT         1834 a         2073 c         1909 g         1888 t
ORIGIN      
        1 gtacagcctg gtcctgtttg gtccagaggg aaggtccggc acagtaggcg gatcaattat
       61 tctgaacgac atcaaatcag tggctgagcc gagctgcccc gatatcttgg cagattcccg
      121 acgctccagc agcttctcat agtttgattt gttcacctgg tagtcgcgat tgagttgggc
      181 aagctccgcc tccacttgag gcaccgcatt gcttaaagac ctcaagcgat tgtagcggtt
      241 agtatactca tccacgcgtg ctttcatgga cgccacatcg gcttcagctt cggccagcgc
      301 cacattcagt tgctgcagca ttgggctata gtttttccca gggtcccccc cgctggggcg
      361 aatcagctta gcttcttccc tcttgcgttc ctcaagctgg gcaatgagtc tctttgtcga
      421 aataatatca ggatgaagtt ccgtatagtt cagcatgaga atgtcaagat ttttattgag
      481 ctctgcgatc cgcgaatcaa gctccgtgtt ccccgctatt gagcttgact cgtcctgatc
      541 gacaaggagt accggttcat ctcccgtgat ttggcgcttg atcgcgtccc gggcctgctc
      601 ggcctcccgt aattccagtc tcgccttatt tatttcttcc tccgcctggg agagctgaga
      661 ataatagtcg cttccctgct gaggcatcaa tccgatgttt ttctgcttaa aagccttgag
      721 gttattctca ccggcaatca gtttctcttc ataactctgg atctgttcgt caagaaaacg
      781 gattgcggaa gaagaatcgt gcttgtcacc aagaccccct tccacaaaaa tggtcaacaa
      841 cgactggacg acatccttgg caagttttgg acttcggttg ctgtaggaaa tcgtaaaaag
      901 attatcgcgt ccggtggtcg caagctgaat cttttccatg agctccttga cctgcttttc
      961 ctgatccttc acgtcctttg ccttgatatc cagatcgacc atgcgaatga cgcgctcgac
     1021 attcggacgg ctgatcaggg tgcggctcat gatggacact tgctgctgca gatccggaga
     1081 aactgtcatg ccggacatca gcggtttcag gacgttctga gtgtccacgt aaattcttgc
     1141 ggacgcctgg tagtcgtcag gcagcttgta aacgatgacc cagcctccaa ccgcaacgat
     1201 ccaggccgca atcactgcaa tccatcgata cttccatatc cctttcagat aaaccagaag
     1261 ctgggttgac agttcctcca tattctcggg ggtcctcggg atgcctttac tttaatgggg
     1321 tgtggttgac ggtcgggact gcgctaacgc gcaattttcg gggaacactg tcttcctgct
     1381 attgttcgac gcagactgtg cagatgaagg gaaaaatcag aaaaagctct cggggatgac
     1441 cagaacgtca ccctgacgca tcggcacatt ggccgagatg tccccgtccc ttatcaaatc
     1501 atcaaggcga acgctgaatt gttgttgttt tccatccact acacgaatga ttctcgcctt
     1561 gtttcctgcg gcgaagtcgg tcatgcctcc caccgcgata agaacgtcca tgagcgtcat
     1621 ttcttctcta tatggcagcg cctgaggctt ggtcgcttca cccactactc ggatttgctc
     1681 cgtatacggc ccgacgaact ccgtcacgac aaccgtcact acgggctgct gaatatactt
     1741 ggataaagct tgttcgatgt ctcgcgccaa ctgagtggac gtttttccgc tcgcttgcag
     1801 atcttctacc agcggagtcg tgatttttcc atcggggcgc acgggtacgg ccatggaaac
     1861 ttcgggattt cgccagacga tcacgtccac attgtctcca gggccgataa tgtaatcatg
     1921 cgacagatac ttttcactgg aactgagcgt aggatagctt ttgcagccgc caagcgagag
     1981 aatgggaaag actgtagcaa gtaaagccag ccacttaaag atatgcagaa ttttcactga
     2041 attccccttt ttattgtgga agatggcgtg gattacgcga gaattgacca tttttagaaa
     2101 ataaagatgc tataagtgaa cgtgcctgtc aagacagaat ttatttatta tccgcaggat
     2161 ataatcacga acgtacccgt agccctcatt ccacaatagt tgtcaaattc caggatgagg
     2221 taaaaaatcg agcttccgca tggaaagttt ggtctgacga agaacacttg tccaaaaact
     2281 accggcggac attctaccct aaccgccatc ctaccaagtc caggatttga agcaaactgg
     2341 actttggtac accctctctc ccgttccatt ttccatgggg aacgagttta catatatata
     2401 cgccctccgg tttgtttaaa attggggcta aacgccgcgt ttatcctggc tttgcaacgc
     2461 ccactgcaca tgttcccgaa caagcgccga agcatcatcg cgcctcgcct ccaaaacctt
     2521 agccacatcg cttgaagcgg gcgcatttcc caagcctatt gccaggttgc gcaaccattg
     2581 ctcgtaacct atccgccgga tagggctgcc agcaagcctg gtgtcgaact cttccttgct
     2641 ccatccgaat aactcaatga ggcttacgtc atcgagcccg ttacggaccg cgaaatcttc
     2701 ttcctcggtt tttaccgcaa atttgttcca gggacatgcc agctggcaat cgtcgcagcc
     2761 gtatacacga ttgccgatca atgggcgcaa gggttcggga atgctgccct tatgctcgat
     2821 ggtcaggtac gaaatacagc gtctggcatc cagccggtaa ggcgcgagga tcgcgccagt
     2881 gggacagatg tcgatacact tcgtacagct tccgcagtag ttccccacag ggtcgtcgac
     2941 tggcaagggc aaatcggtgt aaatttctcc caggaaaaac atcgaacccg cattccgtga
     3001 taaaagcaaa gtatgcttgc cacgccagcc cgtgcctgct ttttgcgccc actccacctc
     3061 catgaccggc gcgctatcgg taaacacgcg gtaattgaaa tgctccactt cggctgaaat
     3121 ccgttcagca agcttctgca ggcgcgcccg caggacttta tgatagtcac gcccgtgcgc
     3181 gtagcgagat atgaaggcct tgtttccatt ttgtattact ttccagctgt ctgctgcgcc
     3241 aggcatataa ttcatgcgaa cagaaatcac ccgaacggtc ccgggaacga gttcatcggg
     3301 ccgagtgcgg cgggtaccgt gctttcccat ataatccatc tcaccctgat atcctttgct
     3361 cagccactgc tgcaagccat gctccgactc cgtcatgtcc gccttggcgt gcgcgacgcg
     3421 catctcctgg aaacccagtt cctttgccca agcctttatc cttttcgtga gcagtacaag
     3481 atcaggggaa gaatagccat cgtcgccaga cagattatcg aggtcttctt ttacttgatt
     3541 catctcatct ccatccttca tgtttcatcc gcgtgattac cttgcgctct gtctcgctga
     3601 tgaatttgcg acgattgagc tcggaaaaaa aatagcagca atcatacgtc cgggtctgat
     3661 catttttcta acgggaaacc tgggagcagg caaaacgacc ctggttcgag gtattctgcg
     3721 tggcctgggt tacgtggaaa aagtgaagag tcccacctat aatctgattg aagtttataa
     3781 aatttctagg ttatacttgt atcactttga tttttatcgc ttcaatgacc ctttcgagta
     3841 cgaagaagcc ggttttcggg agtatttcaa ctcagagtcg atctgttttg tagaatggcc
     3901 tgaaagggca gctggattat tacctgccgc tgaccttagt ctttttttcg agatctcggg
     3961 tacgaagcgg gtcgtgaaaa tttatccagg cacggaagca ggcaacggat gccttgaata
     4021 cttgagccac tagtaagatg caccaagccg ccctgaaccc acatacgcag gatcgccgtc
     4081 atagcttcgc gctgcaaaca gccctgagtt cggtcttcgt cgcactgatg ttcctggcat
     4141 tcgagaatct cctctttgca gccccaattc ctcaaagcat atccatcagt tccgcgcgag
     4201 tctggcctgc tcccgaatat acgcgtctga ctttggaagc ttccctgccc attcagtatt
     4261 cgcttaatac ggttaaaaat ccccaccgca tcgtcattga cctcgaacac gtcctcctta
     4321 cgggagagat tgaaaagctg ggggaaagaa tcgattcatc cgacccctac gtccgctcga
     4381 ttcgagtcgg ccgttataag cccgggatcc tcaggctggt cgtagacctg aagaccgagg
     4441 cagcgcctca agcgtttatt ctcaaacccg tgggcaacta caagcacagg cttgtgctgg
     4501 atatctaccc ggtcgttcct cccgatccgc ttatggcttt cctgagcgag aacatcactg
     4561 ccgtgttgca ggatgacgca gcctctctca agaccggtaa caaggcagcg atgaatgacg
     4621 attcacgctc gggaaccgta agctcaaaga aagcaaaacc tcaaactgct cgtcttatta
     4681 cagttgccat cgacccaggc cacggcgggg aagatccagg ggcgataagc cgcagcggca
     4741 cgaatgaaaa agatattact ttagcaatag caagaaaact caaagccaga atagacaaag
     4801 aacccaacat gcgcgcggcc ctgaccaggg acggcgatta ttttatttcc cttcccatgc
     4861 ggctggtcaa ggcacgtcag tggaacgcgg atctgttcgt ttccgtacac gcagatgctt
     4921 tcataaaacc acatgctcga ggctcttcag tattcgcgct gtccgagcgg ggtgcaacct
     4981 ctgccgccgc ccgctggctt gcgaaaaaag aaaatgaggc agatctgata gggggagtaa
     5041 acctcgacat caaagacccg tatctcaagc aaacattgct ggatctctcg caaaccgcaa
     5101 ccatcagcga cagcctcaaa ctcggcaagg aggtgctgtc agaaatcggc gacatcaacc
     5161 acctgcataa aaacgacgtg gagcaggctg gatttgccgt gctcaagtct ccggatattc
     5221 catccattct ggtggaaact gcttttatca gcaaccctga cgaagaaaag aagcttaagg
     5281 acgctgctta ccaggagagg cttgccgcag cgatcgtagt cgggatcaaa cgatattttg
     5341 ccaataatcc gccagccgca cggtcgaaaa tggtatcgct tgactgagcg cgtcgtgcca
     5401 tgcgggcttg gcctcctcag tatgtgacga gcagatcatt tacggatttc gtcaatcgtg
     5461 gctatgtgaa aaccgagttt tccctgtcgc cgctcctgaa agtagcgttt caacggctca
     5521 tcaatcacgc ggaaggcaag ttgatcccac ggcatttctt cctcctcgaa aagcctgaca
     5581 tcgaggctct cgataccggg ctcgaagtcc aggtcaagaa gttgtgcccg aaagaaaacg
     5641 tgcacttggt tgatgtgagg tagattgtaa atcgcgaaaa ggtcgcccat ttgtaccctg
     5701 gcattcgctt cctccaatgt ctcgcgtgca gcgccttgtg ccagcgtttc ggcattttcc
     5761 atgaatcctc cgggaagagt ccatagacct ggacgcggtt cgatcgccct gcggcacagc
     5821 aatatctttt tctcccactc gggtatgcag ccaacaacta tcttcgggtt ctgatagtgg
     5881 ataacgccac aggcggtaca gacatggcga agcaacttat caccctcggg gattttcaac
     5941 tctacggtcg taccgcagtt actacagtat ctcatttata tttttaaagg gaatcgaacg
     6001 gatgccaata taatggcaca tatttgtttc gagacaatct tgattccgca atacgttgaa
     6061 ccttggcgga atatccctta cgtgattgaa gcgctcgcag cgctactcat gaatctacac
     6121 gtttccccgt gaaaatcgcg attgcacaaa ttaattgcgt cgttggcgac ttcagcgcca
     6181 acatcaggaa gatcctcgac tactgcaatc gggcccggca acaagaagcg agccttgtgg
     6241 tcacgcccga gctcgcgctg tgcggatatc ccgcggaaga tctcctgttg cgtcctggat
     6301 ttgtcaatgc ctgcgcagag gcgcttgcag atttgagcag gcaggttgaa ggtattacgc
     6361 tcctcgtggg ccatccatac tgttcggggg gcaagctcca taactcggcg tcagtcataa
     6421 gcgagaagag gatcattgcg acctatctca agaatctgct tccggaagag agcgtgtttg
     6481 atgaactccg ctatttccag ccaggattgg acccctgcgt gttcgaactg gatggaagaa
     6541 aattcggggt caatatttgc caggatatct gggaacaagg tacagccatt cgtgcgaaaa
     6601 atgccggagc cgactttttg cttgtgctga actcttctcc ctatcacttc cggaagcagg
     6661 acttgaggca ccgtattgtc cgcgagcgca tcagcgaaac gggaatgccc gtggtttatg
     6721 cgaatctcgt gggtgggcag gatgaactgg tatttgacgg agcatcattt gccatgaacg
     6781 aaaaaggcga agtgacccac cagtttgatg cgttcgagga ggtactcggc ctgatcgaat
     6841 tgcaggataa tcaattggtg caagggcagg tttccgcatc cggaagcgtg gaagaaagca
     6901 cttaccgggc gctatgcctt ggcataaagg attatatcgg gaagaaccga atccctggcg
     6961 tcctgctggg gttatcgggc ggggtagatt ccgcactcac gctcgcgctg gcggtcgatg
     7021 cgcttggacc agacaaggtc gaagcagtga tgatgccgtc ccagtttacc actgatatca
     7081 gcttggctga tgctcgcgca attgctgaag cactgggggt gcgctaccgt gaattgccga
     7141 tagattcgat ctttgataga tttctaggca cgctggccag cgcatttgaa acgcaatcgg
     7201 atcaaaagtc tcccgacctc accgcagaaa accttcaggc acgcgtacgc gcagcactgt
     7261 tgatggcctt gtcgaataaa acaggatcta tcgttctcac aaccggaaac aagtccgaga
     7321 ccgcggtcgg ctactgcacc ttgtacggag atatggcggg aggatttgca gtgctcaaag
     7381 atgtcaccaa aaccatggtc tatcggctct gccactaccg caacagcctg tcgagagtaa
     7441 tcccggaaag agtaatcact cgggcacctt ctgccgaatt gcgtcccaac caaactgatc
     7501 aggacagtct tcctccctat gcattgctcg acgccatact ggaggcctat gtcgagcatg
     7561 acttatcatt gacggaaatt ctgcggatga attatcccga agcggatgtt cggcgcgtgg
     7621 taggtctcat tcggcaaaat gaatacaaac gccaccaatc tccccccggc atacgagtta
     7681 catcgcgggg gtttggcaag gatt
//
LOCUS       DASSHJ010000226         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_922111, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000226 DASSHJ010000000
VERSION     DASSHJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922111"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..750
                     /gene="aroQ"
                     /locus_tag="VFI43_08785"
     CDS             295..750
                     /gene="aroQ"
                     /locus_tag="VFI43_08785"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7062258.1"
                     /translation="MKLASSGVKKVLVLHGPNLNLLGVREPRVYGSATLDEINGNLSN
                     LAVSAGIKLASFQSNSESELIDRVQQTIHEGVDFIIINPAAFTHTSIALRDALAAVRL
                     PFIEVHLSNVFAREQFRRESYFSDLARGVISGLGPKGYELALQYVLTAR"
     gene            803..1270
                     /gene="accB"
                     /locus_tag="VFI43_08790"
     CDS             803..1270
                     /gene="accB"
                     /locus_tag="VFI43_08790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET7062259.1"
                     /translation="MDLRKLKKLIDLVEESGIAELEITEGEEKVRISKSPSGMQSHIF
                     STSQPVLPVVGQTGAPGSLPSPQAGVPSVEKLPEGHVVKSPMVGTFYRSSAPGANPFV
                     EVGQTVKEGETLCIIEAMKLLNEIESDKSGVVKAVLVENGQPVEYGEPLFVIE"
     gene            1356..2711
                     /gene="accC"
                     /locus_tag="VFI43_08795"
     CDS             1356..2711
                     /gene="accC"
                     /locus_tag="VFI43_08795"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7062260.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVAVHSQADADAKYVKL
                     ADESVCIGPAPSAQSYLNIPAIISAAEVTDAEAIHPGYGFLAENADFAERVEKSGFVF
                     IGPRPETIRLMGDKVSAKNAMKKAGVPCVPGSNGALPESLEEVRAIIRAIGYPVIIKA
                     SAGGGGRGMRVVHTEAALSNALVITRNEAQAAFGNPMVYAEKYLENPRHIEFQVLADE
                     HRNAIYVGERECSMQRRHQKILEEAPALGISPRLRDRIGTRCADACRRIGYRGVGTFE
                     FLFERNEFFFIEMNTRLQVEHPVTEAISGIDLVQAQIRVAAGEKLTLRQRDITLRGHA
                     IECRINAEDPYKLTPSAGRITQYHSPGGPGIRVDSHVYNNYVVPPYYDSLIGKVIAYG
                     DNREQAIARMRIALSEMVIGGINTNIPLHLDLLSDAAFLNGATGIHFLEQKLANYNKP
                     G"
     gene            2803..3756
                     /gene="prmA"
                     /locus_tag="VFI43_08800"
     CDS             2803..3756
                     /gene="prmA"
                     /locus_tag="VFI43_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7062261.1"
                     /translation="MPWIALAIPTDREHAEALSEAMLELGALSADIHDAAAGTDYEQP
                     LFDEPGEEPGRLWKASELTALFNADADIPAIVQSAAEAVHLPSLPVYSVTQVEEQDWV
                     RLTQSQFEPIKISSRLWIVPSWHPAPDPAAINLILDPGLAFGTGSHPSTQLCLAWLDS
                     NLHRGEHVLDYGCGSGILAIAALKLGAHHVTGVDIDPQAITASRNNAIFNQCDESNIE
                     FQMAHFAARGGHQKEPVADVIIANILANPLIILAPILAASCRTGGRVALSGILSEQAE
                     RVMSGYRQYFKMHIAGEQAGWVLLTGTKVENSIESNHGAYC"
     gene            complement(3841..>4160)
                     /locus_tag="VFI43_08805"
     CDS             complement(3841..>4160)
                     /locus_tag="VFI43_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062262.1"
                     /translation="SGISCATAVSGTSASGVTAPNEVSAGAIARSGSAFGLLAGDAAG
                     SGLETVRSLARFAGAGASDTRFADLRTSDTSGADTSGTGPAAKFPVSGSCTVDNLEYA
                     LNT"
BASE COUNT          959 a         1128 c         1138 g          935 t
ORIGIN      
        1 ttataaagct ttgacgaagg gccggcaaac gcgttcgcag aagtactcca cctgccgttc
       61 agacttcaga acttcctgct tgtagccgca atacgtcttt ctagttgctg tcagcgtgct
      121 gcggcaaggg aattgcctcg tacaacagcc ttaaatcgtc atttttagct gagatacccc
      181 tttccgatcc tctttcaatc gtctgacaac actttctcca ttattttctt gtattttcgg
      241 tgaacgtgcg ctaatactgg acaataggct acggttgagt cacacttgcg tcccatgaaa
      301 ctcgcatcgt ccggtgtaaa aaaagtcctg gttctccatg gacccaatct gaatctgctg
      361 ggtgtacgcg agcctcgggt ctacggtagc gcgacgttag acgaaattaa cggcaactta
      421 tcgaatttag ctgtttcagc tggtataaaa ttagcatcct tccaaagcaa ctcggagtct
      481 gagctcatag atcgggttca acagacgatt cacgaggggg tggacttcat tatcatcaat
      541 cccgcagctt tcacgcatac cagcatcgcg ctacgcgatg ctcttgccgc tgttcggttg
      601 cctttcattg aagttcatct ctccaatgtt tttgctcgtg agcagttccg tagggaatcg
      661 tacttttccg acttggcgcg gggcgtgata agtggtttag gcccgaaagg ttatgagctt
      721 gccttgcaat atgtcttgac tgcccgatga ttcctcaact gttgccaatc atgcatacac
      781 actcttttcc gccgaggtga ttatggattt gagaaagctt aagaaattaa tcgatctggt
      841 tgaggaatcg ggcatcgccg aattggagat caccgagggg gaagaaaagg tacgtatcag
      901 caagtctccc tcagggatgc aaagtcacat attttccaca tcccaacctg ttttgccggt
      961 tgttgggcag acaggtgctc cgggaagcct tccgtcacct caagccgggg ttccatcggt
     1021 tgagaagtta ccggaagggc atgtggtgaa atcgcccatg gtaggtacgt tttatcgtag
     1081 ttccgccccc ggcgccaatc cgtttgtcga ggtggggcag acggtaaaag agggggaaac
     1141 cctatgtatt atcgaagcaa tgaagcttct caacgagatc gagtccgata aaagcggggt
     1201 ggtcaaagct gtcctggtcg aaaatggtca accggtcgag tatggcgagc cgctattcgt
     1261 gatcgaataa cccgtgcaac agtttcttga tgattcgatt gagaagttcc agtctcaaat
     1321 tcagtgtcaa atcctgccat aacaaaaccc ggtaaatgtt cgaaaaaatt cttatagcca
     1381 atcgcggcga gattgcgctg cgcattcagc gtgcgtgccg cgaaatgggc atcaagactg
     1441 tggccgttca ttctcaggct gacgccgatg caaaatacgt gaaattggcg gatgaatccg
     1501 tctgcatcgg gcctgccccc tccgcgcaaa gttatctcaa tattcccgcc attatcagtg
     1561 cagcggaagt gactgacgcg gaagccatcc accccggcta tggtttcctt gcggaaaatg
     1621 ctgatttcgc tgagcgcgtt gaaaaaagcg ggttcgtgtt tatcggcccg cgtccggaaa
     1681 caatccgcct gatgggcgat aaggtcagcg ccaagaatgc aatgaaaaaa gcaggcgtcc
     1741 cctgcgtacc cggctcgaat ggggcgctgc cggaatcact ggaggaagtc cgggcgatca
     1801 ttcgcgcaat tggttatccc gtcattatca aggcgtctgc cggcgggggc gggcgtggaa
     1861 tgcgggtggt gcacacggaa gctgcgctct cgaacgccct cgtaatcacg cgaaacgagg
     1921 cacaagccgc gtttggcaat ccgatggttt acgcggaaaa gtacctcgag aacccgcgcc
     1981 atatagagtt ccaggtattg gctgacgagc atcgcaatgc catctacgtg ggtgagcggg
     2041 aatgctcgat gcagcgccgt catcagaaaa tccttgagga agcccccgct ttggggattt
     2101 caccccggct gcgggatcgg atcggcacgc gctgcgctga tgcgtgccgg cgcataggct
     2161 atcgcggcgt gggtacgttc gaattcctgt tcgagcgcaa tgaatttttt ttcattgaaa
     2221 tgaatacccg tctccaggtc gagcatcccg ttacggaggc gatcagtggc atcgacctgg
     2281 tgcaggcaca gatccgggtg gcggctggcg aaaagctgac cctccgccag cgggacatca
     2341 cgctacgggg gcatgccatc gagtgccgca tcaacgcaga agatccatat aaacttacgc
     2401 catcggcggg ccgcatcacg caataccact cgcccggagg accagggatt cgggtggatt
     2461 cccacgtgta caacaactac gtggtgcctc cctactacga ctcgctgatt ggcaaggtga
     2521 ttgcttacgg cgataatcgc gagcaggcga ttgccagaat gcgaattgcg ctctcggaga
     2581 tggtcatagg tggaatcaat acgaatatcc cgttgcacct ggatttgctg tctgacgcgg
     2641 catttctcaa cggggcaaca ggcattcatt ttcttgagca aaagcttgcc aactacaaca
     2701 agcctggcta gaggctctac caccatatcg tcagagctct caaccgcgac ttcgcactag
     2761 ctgctttcgc actagccttc caagtacaag agactgcctg aaatgccctg gatcgcgctt
     2821 gccataccaa ccgatcgtga gcatgccgaa gccttgagcg aagcgatgct cgaactgggt
     2881 gcattgtcgg cggatattca cgacgcggct gccggtaccg actacgagca gcccttgttc
     2941 gatgaaccag gcgaggagcc gggcaggctc tggaaagctt ccgagctcac cgcgttattc
     3001 aacgccgatg cggacatacc ggcgatcgtg cagagtgcag ccgaagctgt ccaccttccg
     3061 agccttcccg tgtactcggt cacgcaagtg gaagagcagg actgggtgcg cctgactcaa
     3121 tcgcaatttg aaccgatcaa gatatcctcg cgcctttgga tcgttccgag ctggcatcct
     3181 gcacccgatc ctgctgcaat caatctgatt ctcgacccag ggcttgcctt cggcacaggc
     3241 agccaccctt ccacccagct ttgtcttgcc tggcttgact caaatctgca caggggtgaa
     3301 catgtgctgg attatggttg tggttccggc atacttgcca tcgccgcatt gaagctgggc
     3361 gcccatcatg taacgggcgt ggatatcgac ccgcaggcga tcacggcaag ccgcaacaac
     3421 gccattttca atcaatgcga tgaatcgaat atcgaatttc aaatggcgca ttttgccgcc
     3481 cggggaggcc atcagaaaga acccgtggcc gatgtgatta ttgccaacat tcttgcaaat
     3541 cccctcatca ttctcgctcc catccttgcg gcttcctgcc gcactggagg ccgggttgcg
     3601 ctctcaggta ttctttcaga acaagcggaa agggtgatga gcggctaccg gcagtacttc
     3661 aagatgcata tagccgggga gcaggccgga tgggttttgc tgaccggaac gaaagtggaa
     3721 aattcaatag aatccaacca tggcgcttat tgctagctgt ccgcattgcg gcacccattt
     3781 ccgagtcaca ccgctccatt tacaggcgca tgggggggac gtgcgttgcg ggcgatgttc
     3841 tcatgtgttc agcgcatatt ccaggttgtc cacagtgcag gaacccgaaa ccggaaattt
     3901 agccgcaggg ccggtgcccg aagtatcggc acccgaagta tcggaagtgc gcaagtctgc
     3961 aaagcgagta tcggaagcgc ccgccccggc aaagcgcgcg agcgagcgca cagtttccaa
     4021 acccgaaccg gctgcgtccc ctgcgagcag accaaaggcg gagcccgatc gcgcaatagc
     4081 gccggcggaa acttcattcg gagcagtaac tccggaagct gaagttcccg aaacagcagt
     4141 agcgcaggaa atgcctgaag
//
LOCUS       DASSHJ010000227         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_50902, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000227 DASSHJ010000000
VERSION     DASSHJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50902"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFI43_08810"
     CDS             <1..426
                     /locus_tag="VFI43_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7062263.1"
                     /translation="VLRDRSIETRSAAITPPDLDDRTRIRLGAGNYDAMCSPCHLSPA
                     SKESELSRGLYPKPPSWREIGQVDARQAFWIIKHGIKMSGMPAWGKSMSDPHIWNIVA
                     FVKKLPQLTLDSYHELVENSPGHVHDSDDVHSHEPAPSR"
     gene            734..1351
                     /locus_tag="VFI43_08815"
     CDS             734..1351
                     /locus_tag="VFI43_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7062264.1"
                     /translation="MKLFTARILAATTLLGCGLIQPVSAEIYSFTDENGGVHFSNVPA
                     DDRYVLLDGLGRPIGKKTGSLGKSSSNKVSKAQYEAAIDTVARTYSLESALIHAVVSA
                     ESGYNPTAVSRKGAAGLMQLMPETAQRYGVADRFDPVQNLHGGARYLRDLLRMFDGNL
                     SLALAGYNAGEKNVVKHGYQIPPFQETKAYVPKVLNLYRKYQVRM"
     gene            1665..2348
                     /locus_tag="VFI43_08820"
     CDS             1665..2348
                     /locus_tag="VFI43_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062265.1"
                     /translation="MLIWISSGKSSNDRSPSQSKEEDLENWKRVRTAIEHENNLVNQR
                     LSWLFTSQALLFSAFALILTTFLKEEAAQFSPAHPATEYVSLAPYVSIILVVLAVTGG
                     YIYMAVGVSLLAAHRQLCALTDWWDDHCTDKSGHPELHNFLAKESPSFVSAENVPFVF
                     FGAWLLLLSFSVLAVLHFVYDRNLIELNSSTVASAFLIFLIIGIITWAIKEGLSMNYQ
                     RHYSVKRRK"
     gene            complement(2470..4287)
                     /gene="lpdA"
                     /locus_tag="VFI43_08825"
     CDS             complement(2470..4287)
                     /gene="lpdA"
                     /locus_tag="VFI43_08825"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7062266.1"
                     /translation="MAQPIEIKVPDIGDFHDVSVIEVLVKPGDKVEKETPLISLETDK
                     ATMEVPSPMEGVVKEVNVKVNDKVSEGSLILKLEQSAPATQTPQTAQPGQPSETEPVE
                     LTEKGVEPRVTTAALSPETAPPHGTISPAEGDVHADVVVLGAGPGGYTAAFRAADLGK
                     KVVLIERYPTLGGVCLNVGCIPSKALLHIAKVITDSEEAGRQGINFGKPGIELDKLRN
                     WKDSVTGKLTKGLATLAKQRKVTRIQGTGKFTGPNMIQVETSEGLKNISFEHCIIATG
                     SSATRIPGFPYDDPRLMDSTGALKLEDIPKRMLIIGGGIIGLEMATVYDALGSRVSVV
                     ELMDQLMPGADADLVRPLHKRIQKRYEAIHLKTKVIRIEAQQDGLKVTFEGASTDTKV
                     PEPQLYDRILLAVGRRPNGKGIGAENAGVQVNERGFIPVDKQLRTNVPHIFAIGDIVG
                     EPMLAHKASHEGKVAAEIIAGHKTAFDARAIPSVAYTDPEVAWMGLTETEAGKQGIEY
                     EKASFPWAASGRALAMARDEGMTKLLLDKKTRRLLGAGIVGVNAGELISETVLGLEMG
                     ADMEDIALTIHPHPTLSETVFFAAEIAEGSITDLYMPKK"
     gene            complement(4370..>5044)
                     /locus_tag="VFI43_08830"
     CDS             complement(4370..>5044)
                     /locus_tag="VFI43_08830"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET7062267.1"
                     /translation="MKEFVDIIVPRGGKGLIERISREARVPVIKHLDGVCYVYIDEGA
                     DLAKAIRVSDNAKTQRYGTCNTMETLLVHTAIAAEVLPALCKIYLDKGVELRGDAEAR
                     ALVPEMREATEEDWYAEYLAPILSVRVVKDLDQAMDHIATYGSQHTDSIITEDYSRAR
                     RFLREVDSSSVMANASTRFSDGFEYGLGAEIGISTDKLHARGPVGLEGLTSQKFIVLG
                     DGHIRQ"
BASE COUNT         1112 a         1388 c         1331 g         1213 t
ORIGIN      
        1 gttttgcgag atcggtcgat cgaaacccgc tccgctgcga taaccccacc cgatcttgat
       61 gaccgtacga ggatcagatt gggtgcaggg aattacgacg cgatgtgctc gccttgtcat
      121 ctttcgccgg ctagtaagga atccgaactg agccgcgggc tttaccccaa accgccctcc
      181 tggcgggaga tcggccaggt tgacgctcgc caagcgttct ggattatcaa gcacggcatc
      241 aagatgagcg gcatgcctgc atggggcaag agcatgagcg atcctcacat atggaatatc
      301 gtggctttcg tgaagaagct gccccaactc acgctggact cctaccatga actcgtcgaa
      361 aacagcccgg ggcacgttca tgatagcgat gacgttcact ctcatgagcc tgccccatct
      421 cggtagccgg tgaagttgaa tgctccttgt tttccagttc aatccatcag ctatccgtaa
      481 gacttaggaa aacctaggaa atgacagggt tgagtattcg gaataaaacc cacgccttaa
      541 acagctgatt ttgcggaatt gccaaaggat tgccggtcag ggactgccga tccgctatat
      601 ctggtacatt tacccaagct catgctgatt taggctaata ttttgcccgt atcaattcat
      661 cgagacgcgc gacggcaggt ataatcggaa aaccgaattc acatagaaga gacagggaac
      721 gcggaagacg gggatgaagc ttttcacggc gcgtatactc gcggctacaa ccttgctggg
      781 atgcggcctg atccagccgg tatcggcaga aatctactcg tttacggacg agaacggcgg
      841 agttcacttc agcaacgtcc ccgccgacga ccgctacgtt ctccttgacg gcttgggccg
      901 acctattggt aagaaaacgg gcagccttgg caaaagctcg agcaataaag tctcgaaagc
      961 tcaatatgag gccgctatag acacggttgc ccggacttac tcactggaaa gtgccctcat
     1021 acatgcggtg gtgtccgcag aatcaggata taacccgaca gcggtttcca ggaaaggcgc
     1081 agccggactg atgcaactca tgcccgaaac ggcgcagcgc tacggggtgg ccgatcggtt
     1141 cgaccccgtg caaaaccttc acgggggcgc ccgttatctg cgcgacctgt tgcgtatgtt
     1201 tgacggcaat ttaagcttgg cgctggctgg ctataatgcg ggagagaaaa acgtggtcaa
     1261 gcacggatat caaatccccc cgttccagga aaccaaggca tacgtgccga aggtgctgaa
     1321 cctctatcgg aagtatcagg tgcggatgta actgtccaat cagcgcgtag cagaaatgct
     1381 atctcgcccg gtttcccgtg gaagagcatt attatttgcg gtaattacat tgctgtaaaa
     1441 atcgctcagg attagcgtct catccttcct acaggcgttg agacgtctat atggtgttta
     1501 tgaatcgctt tcatagttca cgttgcgctc tttgctatat cagaataact catgatgtaa
     1561 gcgaatgtct gttcaaactt gcttgtcgtc gtagtgattg ttattgcatt ttctccagac
     1621 gaagttccac caattccccc gagtactaac caggagaata ggagatgctt atctggatat
     1681 cgtctggtaa gtcgagtaac gacaggtctc cgtcccaatc aaaggaggaa gatcttgaaa
     1741 actggaagcg agtccgtacg gcgattgaac atgagaataa tctggtaaat cagcggctaa
     1801 gttggctgtt tacctcgcag gcgcttttgt tttcagcatt tgcgctcatt ttgacaacgt
     1861 tcctaaaaga ggaggcggct caattttctc ccgcccaccc tgcaaccgag tatgtgtctt
     1921 tggccccata cgtatccatt atacttgtag ttctggcggt aacgggaggc tatatataca
     1981 tggctgtcgg cgtatcgttg ctcgcggccc atcgtcagct atgtgctcta acggactggt
     2041 gggacgatca ttgcactgac aaatcggggc atccggaact tcacaatttt ttggctaaag
     2101 agagcccttc tttcgtcagc gccgagaacg tcccttttgt attcttcggc gcatggctcc
     2161 tactactctc attttctgta ctggcagtac ttcattttgt ttatgaccgc aatctcatcg
     2221 agctaaactc atcaacggtt gcgagcgcct tcctcatatt cttgattatc ggaatcatca
     2281 cttgggctat aaaggagggt ctaagcatga attaccaaag gcattactcg gtaaaacgaa
     2341 ggaaataaaa gccgacaatt ttcaacgcga agaaaagtaa gattagctcg ataccgatac
     2401 cgataccgat atcggggaga gtacgaaaat ctccctatca ccgctttatg attggagcag
     2461 taccaaagct tacttcttcg gcatataaag gtccgtaatc gatccctccg cgatctcggc
     2521 ggcaaaaaat accgtttccg ataatgtcgg atgcggatga atggtaagag cgatatcttc
     2581 catatccgct cccatttcca gtccaagcac ggtttcggaa atcagttccc ctgcattgac
     2641 gcccacaatt ccggctccca gcagccgccg cgttttcttg tccagcagca acttcgtcat
     2701 tccctcgtcg cgggccatcg cgagcgcgcg tccactcgcc gcccagggaa acgacgcttt
     2761 ttcatattcg atgccctgct tgcccgcctc ggtttcggtc aggcccatcc aggctacctc
     2821 aggatcggtg taagccaccg acgggatagc acgcgcatcg aaagcggtct tgtgccccgc
     2881 gatgatttcg gcggcaacct tgccttcatg ggaggctttg tgcgccagca tcggttcgcc
     2941 cacgatgtcg ccgatagcga aaatgtgggg gacgtttgtg cggagttgct tatccacggg
     3001 gatgaagccc cgctcattga cttgcacgcc cgcattttcg gcgccaatgc ctttcccgtt
     3061 cggccggcgc cccacagcta gcaggattcg atcgtaaagt tgcggctcag ggacctttgt
     3121 atcggtcgac gcgccttcga atgtcacctt gagcccatcc tgctgcgcct cgatccggat
     3181 caccttggtc ttgagatgga tcgcttcata acgtttctgt atgcgcttgt gcagcggtcg
     3241 aaccaaatca gcatccgcac cgggcatcaa ttgatccatc agttccacga cgctgaccct
     3301 gctgccgagc gcatcgtaga ccgtagccat ttccaggccg ataatgccgc cgcctataat
     3361 aagcatgcgc ttcggtatgt cttccagctt tagcgcaccc gtcgaatcca tgagacgggg
     3421 atcgtcatag gggaagccgg gaatgcgggt cgcgctcgaa cctgtggcaa tgatgcaatg
     3481 ctcgaacgat atatttttca atccctcgga agtttccacc tgaatcatgt ttggcccggt
     3541 gaactttccc gttccctgta tacgggtcac cttgcgctgc ttcgccaagg tggccaatcc
     3601 cttggtcagc tttccagtca ccgagtcctt ccagttgcgc agcttgtcca gctcaatgcc
     3661 tggtttcccg aagttgatgc cctggcgccc cgcttcttcc gaatcggtga tcactttagc
     3721 aatgtgcagc aacgcttttg aggggatgca accaacattg aggcatacgc cgccgagcgt
     3781 ggggtagcgt tcgatcagca ccactttctt ccccaggtcg gcggcacgga aggcggcagt
     3841 atatccgccc ggtcccgcgc caagtacaac cacatctgca tgaacatctc cctcggcggg
     3901 agaaatggtg ccgtgaggcg gagcagtttc gggcgaaagc gccgcagtgg taactctggg
     3961 ttccacccct ttttcagtga gctcaacggg ctcggtctcg gacggctgac ccggctgtgc
     4021 agtctgggga gtctgagtgg caggagcgga ctgctccagt ttgagtatga gcgagccttc
     4081 cgatactttg tcgtttacct tgacgttgac ttccttcacc actccttcca ttggggacgg
     4141 aacttccatg gtcgccttgt cggtttccag ggaaatgagg ggagtctctt tttccacctt
     4201 atcgcccggc ttgaccagga cttcaatcac tgagacatcg tggaaatcgc caatgtcagg
     4261 gacttttatt tcgatcggct gagccatgtt agagttgggc aagacgaggt aagggtgaag
     4321 aaggggtcaa aagtgggcac gggcgttttt cctggaatcg ggccagcaat tattgccgga
     4381 tgtgcccgtc gcctaggaca atgaatttct ggctggtcaa gccttccagc cccacagggc
     4441 cgcgtgcgtg aagtttgtct gtggagatgc cgatttccgc ccccaaaccg tactcgaacc
     4501 cgtcagaaaa gcgcgtggac gcattggcca tcaccgaact ggagtccacc tcgcgcagga
     4561 agcggcgagc gcggctgtag tcctctgtga tgatggagtc ggtatgctgg gaaccgtagg
     4621 ttgcaatgtg atccatcgcc tgatcaaggt ccttcaccac ccgcacactc agaatgggtg
     4681 caagatactc tgcgtaccag tcttcttccg tcgcctcgcg catttcgggg acgagggcgc
     4741 gcgcctcggc atcaccgcgc agctccaccc ccttgtcgag gtagattttg catagcgcgg
     4801 gcagtacctc ggccgcgatt gctgtgtgca ccagcaaggt ttccatcgtg ttgcaagtgc
     4861 cgtagcgctg cgtctttgcg ttgtcggaaa cccgtatcgc tttcgccaga tccgcgcctt
     4921 cgtcgatata tacatagcat accccgtcga ggtgcttaat gacggggacg cgggcttcgc
     4981 gtgaaatacg ttctatcaat cccttgccac cgcgcgggac gatgatatcg acaaattcct
     5041 tcat
//
LOCUS       DASSHJ010000228        37834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1018934,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000228 DASSHJ010000000
VERSION     DASSHJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 37834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37834
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018934"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VFI43_08835"
     CDS             complement(<1..1017)
                     /locus_tag="VFI43_08835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET7062268.1"
                     /translation="MILAVLIFVLTAGGLLAGVAERGKAGSARWIALITAAIELVLAV
                     QLWLQHSEKITLEGGSWLAEFVAEWIPRFGISFHLAMDGMSLVLIVLTAFLGIVAVLC
                     SWTEIQERAGFFYFNLLWTLAGAVGVFLALDLFLFFFFWELMLVPMYFLIAIWGHERR
                     GYASIKFFIFTQGSGLLMLLAILALVFIHYRSSGNLTFDYFPLLNVVMSDSASFWIML
                     GFFIAFAVKLPVVPFHTWLPDAHTEAPTGGSVVLAGVLLKTGAYGLLRFVVPLFPESA
                     LQFAPVAMGLGAAGVLYGALLAFAQTDFKRLVAYTSISHLGFVVLGVFAWNAWSVGGA
                     VMQMV"
     gene            complement(1014..2915)
                     /gene="nuoL"
                     /locus_tag="VFI43_08840"
     CDS             complement(1014..2915)
                     /gene="nuoL"
                     /locus_tag="VFI43_08840"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7062269.1"
                     /translation="MLALLWLVPALPFAGFMALALGGERLGHRGVAVVGAGSVGLAAL
                     IAILIASGFILDPPPAFAFRQTAWRWMDLAGFTPEIAFYLDPLSLVMILVVTIISFLI
                     HVYSAGFMEGDEGYARFFAYMNLFVGSMCVLVLADNLVLLYLGWEGVGLCSYLLIGFW
                     YKDPANGQAARKAFIVTRVGDTALAIGLFLLFTHLGTLDIQPLMQRAAVIFPPGSGIA
                     AAAAALLLGGAVGKSAQLPLQTWLPDAMAGPTPVSALIHAATMVTAGVYLIARTHVLF
                     SLAPSVQFAVAVIGALTLLLAGCSALAQKDIKRVLAYSTISQIGYMFLALGVGAWSAA
                     IFHLMTHAFFKALLFLSAGAVIMSLHEEHDIFRMGGLRQKLPVAFWTFLIGGASLAAV
                     PVVTAGFYSKDLILTQSWLSTNGGPWLWAAGWVGAILTSLYIFRVIFLVFFGEARGPE
                     LSKRTGLRMSVPMLILAALSIAGGWIETPPFLGNLTLFSNFVRHALPATQFIHGDESG
                     RTELILELLAISASLAGIFLAYLLFLRYPAFLHGLMLMPALARLRKFWHYGWDFDRLY
                     DVLFVRPYIWLARSNQGDVIDNVYSGVARLCLAFYRGLSATQTGLVRQYAAAIAAGSA
                     AIVFIAIFI"
     gene            complement(2917..3225)
                     /gene="nuoK"
                     /locus_tag="VFI43_08845"
     CDS             complement(2917..3225)
                     /gene="nuoK"
                     /locus_tag="VFI43_08845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET7062270.1"
                     /translation="MAAIPMQHGLLLAAVLFVLGMIGILVRRNLVFILMSIEIMLNAA
                     GLAFIVAGARWGQADGQVMFIFILALAAAEVSVGLALLLLLHHGFQTLDADAVNKMRG
                     "
     gene            complement(3230..3820)
                     /gene="nuoJ"
                     /locus_tag="VFI43_08850"
     CDS             complement(3230..3820)
                     /gene="nuoJ"
                     /locus_tag="VFI43_08850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET7062271.1"
                     /translation="MEAAFYLSGAVAIAATTMVITRASAVHALLYLIVSLLALAVVFY
                     VLGAPFAAALEIIIYAGAIMVLFVFVVMMLNLGKAAEAREHQWLSPGMWIGPAVLAAI
                     LLAELIYILARGGATSSGAAVVESKEVGMALFGPYLLGVELASLLLLAGLIGACHLGR
                     HQGMRLNAGEKEGSDESLRRGSWGMARGELRAHEDD"
     gene            complement(3840..4355)
                     /gene="nuoI"
                     /locus_tag="VFI43_08855"
     CDS             complement(3840..4355)
                     /gene="nuoI"
                     /locus_tag="VFI43_08855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET7062272.1"
                     /translation="MWNSLKTLWDIFLHTFRPRVTIQYPDEKPYMPPRWRGRIILSRD
                     PDGQERCVACYLCAAACPVDCIALQATEDEHGRRFPEFFRINFSRCIFCGYCEEACPT
                     DAIQLTPDFEMGEYERKNLVYEKGDLLIDGTGKYPGYNFYRTSGVAIGGKGKGEAERE
                     APPVDTRSLLP"
     gene            complement(4376..5329)
                     /gene="nuoH"
                     /locus_tag="VFI43_08860"
     CDS             complement(4376..5329)
                     /gene="nuoH"
                     /locus_tag="VFI43_08860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET7062273.1"
                     /translation="MNETVGIWINLILILAVLFTFSALLSWVERRLLGLWQDRYGPNR
                     VGPFGTLQAAADAIKLLTKEDWVPPFADRAVFVLAPTIIAAATMLAFAVVPIAPGIGV
                     VDLNIGLLFFLAMSSLGVYSIVLGGWASNSKYSLLGGFRAAAQMLSYEVFMGLALMGV
                     VMLAGSFNLRDIVIAQENLWFCVPQVLGLLTFTIAGIAEARRLPFDLVEAENELVAGF
                     HTEYSSMKFGLFFIGEYVGVTLISAMVVTLFFGGWLGPVLPPLAWFLLKTFVVIVFFV
                     LLRASLPRPRYDQLMAYGWKVMLPLTLVNLLVTGAVVLARS"
     gene            complement(5322..8093)
                     /gene="nuoG"
                     /locus_tag="VFI43_08865"
     CDS             complement(5322..8093)
                     /gene="nuoG"
                     /locus_tag="VFI43_08865"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HET7062274.1"
                     /translation="MATIHIDNRPYPANDQENLLQQCLSLGFNLPYFCWHPAMGSVGA
                     CRQCAVKQFKNAEDRRGKLVMACMTPAKDGTFISIDDAEAHEFRESVAEWLMVNHPHD
                     CPVCDEGGECHLQDMTVMNGHVYRRYRGPKRTFHNQDLGPLVNHEMNRCIQCYRCVRF
                     YRDYAGGRDLDALVLRDKVYFGRHQDGTLESEFSGNLVEVCPTGVFTDKTLRWHYTRK
                     WDLQTAPSVCVHCGSGCNTIPGERYGTLRRIRNRFNGDVNGYFLCDRGRYGYEFVNSE
                     LRIRQPAIRGDKNELPQPVAGSDAVEYLGEILSQSGRVVGIGSPRASVEANFALRTLV
                     GSDRFHTGLNQRESRLLTSILDILRQGPARIPSLHDVELADAVLILGEDVSNTAPRLA
                     LSLRQSVRRQPEKRAERMKIPLWNDSAVREATRHEHGPLLVATVADTRLDDIATQTFR
                     GAPADITRLGFAIANRLDPHVPPVAGLSAEAGAVANAIAEKLKGAEHPLIISGMGCGD
                     IGIIEAAANVARALCNTGRNAALCYAVPECNSLGMAMQGGGDLEAALELLESDAADIL
                     VILENDLYRRSDKVLVERMLAAARHIVVIDHTSHRTSEKAHMLLPAAAFAEADGTLIN
                     NEGRAQRYFQVFVPEGEIQESWRWIRDIMAVAGRGSEANWRNLDDVTAACALAIPGWD
                     RIVHAAPPASFRIDGQKIPRQPHRYSGRTAMRAGVSVHEPEQPRDPDTPLAFSMEGYG
                     GDPPPSLIPRFWSPGWNSIQSLNKFQQEVGGALRGGNPGERLIEPAAQGEVDYFEDIP
                     AEFHSMPDKWLVVPLHHIFGGEELSTLSRGISELSPQPYLALNPGSAAGLGLVPGDSV
                     AVQLIDKPGKMLTAQLPVKIKPELPEGIAGLPVGLPGLGDLLGVELPAWAKVTKAEKR
                     SNGRGAGHE"
     gene            complement(8114..9391)
                     /gene="nuoF"
                     /locus_tag="VFI43_08870"
     CDS             complement(8114..9391)
                     /gene="nuoF"
                     /locus_tag="VFI43_08870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7062275.1"
                     /translation="MEKPLTHDISTGKEPPDLAQYEKTGGYSGLRKALRMAPADVQQV
                     VKDANLRGRGGAGFPTAQKWSFVPMGDDAPRPKYLVCNADEMEPGTFKDRLLLEGNPH
                     QLLEGMIVSAYALQADIAFVFLRWAYKLAAHRMERAIGEAYGKGYLGKNILGSGYSLE
                     LHLHASAGRYICGEETALLNALEGRRANPRAKPPYPQVSGLWGKPTIVNNVETLCNVP
                     HIVDKGAEWFKSLSHTADGGTKLYGASGKVKKPGLWELPMGTPIREILEEHAGGMQDG
                     YQFRGLLPGGASTDFVVADHLDVPMDFDSMQAAGSRLGTGTMIILDDRTCPVGMLLNL
                     EHFFAQESCGWCTPCWSGLSWIEQILRDMEEGHGQPSDLERLASHTRLLGPGHTFCAL
                     APGAAEPLQSGLKYFKDDFERHIHEKRCPWREN"
     gene            complement(9369..9875)
                     /gene="nuoE"
                     /locus_tag="VFI43_08875"
     CDS             complement(9369..9875)
                     /gene="nuoE"
                     /locus_tag="VFI43_08875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET7062276.1"
                     /translation="MLSEQEREEIEAHSRHYPDRRAVCIEALQIIQRHRGWVSDEGLA
                     DVAEALQMTLAELEGVATFYNMVFRQPVGKHVILLCDSVSCWIMGYEKLRDHLTRRLG
                     IEFGQTTADGRFTLLPNVCLGACDHAPAMMVDDAHYQDLDPARIDEILASCELKEGGQ
                     ADGKATDA"
     gene            complement(9884..11680)
                     /gene="nuoC"
                     /locus_tag="VFI43_08880"
     CDS             complement(9884..11680)
                     /gene="nuoC"
                     /locus_tag="VFI43_08880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HET7062277.1"
                     /translation="MTHASSSLALKPGFPDILEEIEARFGPSATRGEAIGPFQQTRDA
                     VPTCWISKSSIPRVLRYLKEEAEKPYRMLYDLTAIDERTRGHREGQPESDFTVVYHLF
                     SFERNAYVRLKVALNSPHLTTPTITALWPAANWYEREVWDMFGVVFDGHPHLRRILLP
                     PGWQGHPLHKDYPARATEMEPFHMSPQREEAEQEALRFHPEEWGMRRAHDGTDFMFLN
                     LGPNHPGVHGVFRIVLQLDGEEIVDAVPEIGFHHRGAEKMGERQSWHTYIPYTDRVDY
                     LGGVMNNLPYVLAVEKLAGIEVPERVKVIRVMMAEFFRIASHLVFYGTFTQDLGQLSP
                     VFFMFTDRERIFDIVEAICGGRMHPSWYRIGGVAQDLPAGWDRLVRDFLDYMPARLRE
                     YDEIALGNRIVKARTQGIGSYSIKEAIEWGVTGPNLRACGFEWDFRKARPYSGYENFE
                     FDIPTGDHGDCYDRCAVRAEEIRQSLRIIGQCLENMPGGTYKADHPLTTPPLKKRTLH
                     DIETLIDHFLGVSWGPVIPPGEALMAIEATKGNNGYYLISDGNTMAYRVRIRTPSFPH
                     LQMIPQISRGLMVSDLIAILGSIDFVMADVDR"
     gene            complement(11813..12364)
                     /locus_tag="VFI43_08885"
                     /pseudo
     CDS             complement(11813..12364)
                     /locus_tag="VFI43_08885"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(12361..12810)
                     /gene="ndhC"
                     /locus_tag="VFI43_08890"
     CDS             complement(12361..12810)
                     /gene="ndhC"
                     /locus_tag="VFI43_08890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET7062278.1"
                     /translation="MNTVQGSPVEIWPLVAYFFLVVMLLATIVGLSYVIGERHKSPET
                     NEPFESGIVPAGLARLRLSAKFYLIAVFFVIFDVEAAFLFAWAIAFRELGWAGYVEAL
                     IFIAVLGAALAYLWRLGALDWGTPEDGRNWRRSRVAGRQELKKGIKP"
     gene            13022..13771
                     /locus_tag="VFI43_08895"
     CDS             13022..13771
                     /locus_tag="VFI43_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062279.1"
                     /translation="MRTTKRRGRTIKADISGQRDLFSVCSLLLKVIVALAILVVNTIA
                     AASADTFRNSCEKTVGAGKVVVTYIDSETIYNSSHSTKSLQSRTAKSSDSYHSIYGLT
                     HAEPSFRLEIRSRLLVNADGQTCMVPDVSVKLGFSAMHVYLAKELGDSCRKNIVREHE
                     LEHVSTWKSHFRIGAKMLKEPLRAAFSRPRHYSSEKEAQADLKPWVEEVLKPLQHKLV
                     NSVMDAQRAIDAPASYERVRQRLYMCLAGNS"
     gene            complement(13885..14508)
                     /locus_tag="VFI43_08900"
     CDS             complement(13885..14508)
                     /locus_tag="VFI43_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET7062280.1"
                     /translation="MNRYLILLAAAITTDSAVAANINGCEIRPAALCIELDLRAADMH
                     GADLARAGFRKSNLSGANLQGANLLNANMDMVNLTGSNLEGAVLNDADLKNATIVGAR
                     LDHAQLNGVRLKSADLTRSDLTDAQLKGANLSGANLTNAKFRNAQLQRADLSGSVMTQ
                     ADFRGANMTGVKLDNATLQGADLRGAVLDEVDFSKLNTNGCIGCPKP"
     gene            complement(14663..15190)
                     /locus_tag="VFI43_08905"
     CDS             complement(14663..15190)
                     /locus_tag="VFI43_08905"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7062281.1"
                     /translation="MKLFAILAALVTMIMTLGSRGAVAEPLKVGQAAPDFNLPDQNGK
                     THKLSDYHGKWLTLYFYVKDNTPGCTKQACKFRDDIHQLRDLGAEVVGISVDDSASHA
                     GFAKKYNLPFPLLADSKGETAKRYDSMWSIIGMAKRNTYLIDPDGRIAKIYLSASASR
                     NSVEVIEDLKRMKGS"
     gene            complement(15432..16205)
                     /gene="djlA"
                     /locus_tag="VFI43_08910"
     CDS             complement(15432..16205)
                     /gene="djlA"
                     /locus_tag="VFI43_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HET7062282.1"
                     /translation="MFKIIGALLGLYFLGFFGALVGYIVGSFIDRYVAYGAGAVNPLT
                     NANRQAVFIQTVFIMMGKLAKADGHITPDEISHVEDFMQKLGMTTEHRQQAIALFKQG
                     AAPDFDYERKLDEFMVVCGHTNSLRQMLLVYLIVMAISDGLMDAREETLLRNCALHLG
                     YDESAFRQLLDMVLNQSHFAGGKAASPTSLDEAYKALGVTRESTDQEIKRAYRKLMSQ
                     YHPDKLMGQGLPEDMIKVATAQAQEVQAAYDLIKKSRNM"
     gene            16385..17107
                     /locus_tag="VFI43_08915"
     CDS             16385..17107
                     /locus_tag="VFI43_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HET7062283.1"
                     /translation="MMIELYSSPTPNGQKVMIMLEETGLEWKHIDVNISLGEQFQAEH
                     LRLNPNNKIPAIIDTDGPGGRPYTMMESGAILVYLAEKTGKFMPRDERRHYDTLQWLF
                     FQAAHIGPMFGQANHFNRQASDNHYARDRYNNESLRLYRVLENRLRETDWIAGDEYTI
                     ADIATYPWCKGHADRGVNKASFPNFMRWFDAMESRQGVQRSNRMATEIRERMDKAAEG
                     RKPIDIYDTKDNGDRLARATRD"
     gene            17413..17829
                     /locus_tag="VFI43_08920"
     CDS             17413..17829
                     /locus_tag="VFI43_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062284.1"
                     /translation="MASFKRFCLAISLAALLAAPQFGCAPYMAAKQPGAKNLELFKTG
                     TPRNALLAEFGAPAVSENRNGTQHEIFRFVQGYSTAARTSRALLHGVADVATFGLWEV
                     VGTPAEAVFSGDEMAYDVTYDKNDRVDQVTILKMDK"
     gene            complement(18139..19179)
                     /locus_tag="VFI43_08925"
     CDS             complement(18139..19179)
                     /locus_tag="VFI43_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAF repeat-containing protein"
                     /protein_id="HET7062285.1"
                     /translation="MLSASLGYVAPVFAGTAAILNPDGKLTYLGTLGGTYSVASGIND
                     SGQVVGHFLTSDGSVHAFITGPNGVGMRDLGTLGGRLSFANGINDAGQVVGYSDTEDG
                     FSSHAFITGPNGVGMRDLGTLGRDYYSNSYATSINNAGQVVGYSYMDGSSHAFITGPN
                     GVGMRDLGALRGGTHSTAAGINDSGQVVGYSSTGSDPKHAFITGPNGVGMRDLGVPGS
                     DSSATGINDTGQVVGNFYTRDGFPHAFITGPDGGGMRDLGTLGGTYSESVAAGINDAG
                     QAVGSSGTADGSVHAFITGPNGVGITDLNSLVVNLPGRNTIDEAYGINNHGQILVYPA
                     KSSPNPRPASCC"
     gene            19743..21407
                     /gene="ettA"
                     /locus_tag="VFI43_08930"
     CDS             19743..21407
                     /gene="ettA"
                     /locus_tag="VFI43_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET7062286.1"
                     /translation="MAQYVLTMNRVGKIVPPRRTILKDISISFFPGAKIGLLGLNGSG
                     KSTVLKIMSGVDKDIEGEVTAMPNLNIGYLPQEPQLNPEQTVRDAVQEGLGELFTAQQ
                     KLEEIYAAYAEPDADFDALATEQSRLEAILATGGGNTSQQMEIAADALRLPDWDTRVK
                     HLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLTRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGHGIPWKGNYSSWLEQKENRLKQEESAESARQKSLKKELEWVR
                     QNPKGRQAKSKARIARFEELNSQEYQKRNETQEIFIPVADRLGNEVIEFKNVSKAYDD
                     RLLIENLNFKVPAGAIVGIIGPNGAGKSTLFRMITGKEQPDSGEIRVGPTVRIAHVDQ
                     SREALDGAKNVFDTISGGHDILTVGKYETPVRAYLGRFNFKGADQQKIVGNLSGGERG
                     RLHLAKMLISGGNVLLLDEPSNDLDVETLRALEEALLEFAGSVLVISHDRWFLDRIAT
                     HILSFEGNSQVAFFDGNYQEYEADKRKRLGEEGAKPKRLRYKPINR"
     gene            complement(21463..21780)
                     /locus_tag="VFI43_08935"
     CDS             complement(21463..21780)
                     /locus_tag="VFI43_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062287.1"
                     /translation="MKTRRLVFRSFVVTSSVILGLTGAGLVHSAGQDIPDEIGPADSP
                     AVRDTNPNNKDKSIKERLHIKEGSTTQKKRDNSRYSTDGTERYDDSDTDITGTGPKRE
                     GRY"
     gene            22175..22783
                     /locus_tag="VFI43_08940"
     CDS             22175..22783
                     /locus_tag="VFI43_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET7062288.1"
                     /translation="MEQEEACPECPVMVSVPGAKFAIGKYEVTFKEWDACVADRGCGG
                     YQPYDNGWGRDARPVINVSWNDVQTYIRWLSQKTGKAYRLPTEQEWEIAMRGGTTTEY
                     YWGNDVGRNNANCDGCGSEWDNRKTAPVGSFKPNPFGLYDMMGNTWEWTDSCWKGNCM
                     KRMLCGGSWNHRPQDMRAGMCNWFDTNKRMRYLGFRVALSLP"
     gene            complement(22839..24089)
                     /gene="murA"
                     /locus_tag="VFI43_08945"
     CDS             complement(22839..24089)
                     /gene="murA"
                     /locus_tag="VFI43_08945"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7062289.1"
                     /translation="MQKLIIQGGIPLLGEVCISGAKNAALPILCASLLTEETLFVDNV
                     PHLNDVTTMLSLLRQMGVQVAVDGKRTQLTASRLSHLVAPYEMVKTMRAAILVLGPML
                     ARAGEARVSLPGGCAIGLRPVDQHIKGLQAMGADIGIEHGYIHAKAKRLSGAHIVMDL
                     VTVTGTENLMMAATLADGITVLENAAREPEVTDLANCLIAMGAKIQGVGTDIITIEGV
                     TALHSAGHRVMPDRIEAGTFLVAAAASGGDVRLLDTRTDTLDAVLDKLMEAGAAIESG
                     EDWIHLRMSTPLKSVNLRTSPYPAFPTDMQAQFMVLNSIAEGTAIITETIFENRFMHV
                     QELERMNADIKVEGNTAVVCGVSALDGAKVMATDLRASASLVLAGLIAKGETIIDRVY
                     HLDRGYEHIEHKLSLLGASIRRAS"
     gene            24475..24864
                     /locus_tag="VFI43_08950"
     CDS             24475..24864
                     /locus_tag="VFI43_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7062290.1"
                     /translation="MKKSTVQTNDAPKPIGPYSQAIRVSGGDTVYLSGQIGLNPSTMQ
                     MAPDVEEQAQQVFSNLMAVAAASGGSLSDIVKLNVYLTDLSNFEKVNAIMASHFKEPY
                     PARAAIGVAALPRGALVEVDGVLVLPG"
     gene            24865..26985
                     /gene="recG"
                     /locus_tag="VFI43_08955"
     CDS             24865..26985
                     /gene="recG"
                     /locus_tag="VFI43_08955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7062291.1"
                     /translation="MADATSIMASPAQAGSIGGALEDKLSRLGIKDELGLLMHLPLRY
                     EDETHLYPIRDAPENRIVQVEGVITHSQVMFRPRRQLVCRVDDGTGVLVMRFLNFYGS
                     QAKAYASGGRVRLVGEIRGGFFGMEMVHPKCRLINGTEPLPDTLTPVYPTTAGLSSSA
                     IHKLVLRALKRVHGYSSAGNEKQEDCLSETLPENILERLGLPGFRNSITFLHQPCPDT
                     PVASLERRTHPAWRRIKFDELLAQQLSMRLHYRRRRSGSAPVLEARNRLAGKLLESLP
                     FQLTKGQKKAVGEISKDLAATFPMQRLLQGDVGSGKTVVAALAMLQAIENGYQAAMMA
                     PTEILAEQHYRKLSKWLLPILEPLGMAIAWISGQQKKSQRESMLNHIARGRAMLAIGT
                     HALFQEQVQFDRLGLVIVDEQHRFGVHQRLALRRKGTGTIGTPHQLMMSATPIPRTLS
                     MSYFADLDVSVIDELPPGRAGIATRLFADTRRDEVIARIRDACQSGRQAYWVCPLIEE
                     SEALQLKTALETRETLSGIFPDLRIGLVHGKLPPQEKSAVMEAFAQGDISLLVATTVI
                     EVGVDVPNASLMIIEHAERMGLAQLHQLRGRVGRGEAGGQCILLYQKPLSANARERLK
                     IIFEHTDGFEIARHDLRLRGPGEFLGARQSGVPMLKFADLEQDIDLLDAARNVADDLL
                     CNHPFEAQFHLERWLGRKSEYLQV"
     gene            27025..27813
                     /locus_tag="VFI43_08960"
     CDS             27025..27813
                     /locus_tag="VFI43_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7062292.1"
                     /translation="MIIQVDNLTLEHPGKTLCRGLSVTINAGECWAILGKNGSGKTTL
                     LNAFAGLGEPKDQADARILADGKPPHVWPRREFSRNLGILLQEEPGEFWGNVEEYVLL
                     GRHPHLRSLFGWDETDHEIAMQALERMELTDVAHRRIGTLSGGERQRARIALLLAQSP
                     SCYLLDEPLQHLDLRHQLATLALFHELTRNGRTIVMVLHDASWANRFCSHVLMLFDNG
                     SATAGPVKEVLNRTNLETLYHCPMHEIGIGGVSHFIPADVAPRV"
     gene            27882..29288
                     /gene="pcnB"
                     /locus_tag="VFI43_08965"
     CDS             27882..29288
                     /gene="pcnB"
                     /locus_tag="VFI43_08965"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HET7062293.1"
                     /translation="MIGKLLRRVFTRGKPPSSPVPGSASGPNVIPREEHGISRDHINK
                     CALNVTSSLQEAGFAAFIVGGAARDLILGLEPKDFDVATNATPEEVRPIFRRSRIIGR
                     RFRLVHVMCGQETVEVSTFRGGSSGEDELASEDHTDDQGRLLHDNIFGTQEEDAKRRD
                     FTVNALFFDPVHEEIWDYLNGYDDIKARRLRIIGDPERRYREDPIRMLRAVRLAAKLD
                     MQIDADTAAPIRDLAPLLRNVPPSRLFDEMLKLLLSGHALTCVAELRKRGLHHGLLPM
                     LDVILEQPLGERFITLALTNTDERVRQEKPVSPGFLFAALLWHEVLGAWNNRQAAGER
                     PIPALYEAMDDVLEVQNKNLAIPRRYDAVMKEIWAAQPRFAARSGRRPFRLLEHPRFR
                     AAYDFMLLRCESGEVDSELGKWWQIFQHANSGEREAMLIRDELLNKRKRSRGRRKPAL
                     ADTVDNGKLTDSDASGPK"
     gene            29291..29809
                     /gene="folK"
                     /locus_tag="VFI43_08970"
     CDS             29291..29809
                     /gene="folK"
                     /locus_tag="VFI43_08970"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7062294.1"
                     /translation="MARIVSSHAVCPAFIALGSNLANPESQVLTAFDELARLRSSRLL
                     THSSLYRSAPVGRLDQPDFINAAAYMETVLSPHELLAALLDIERTHGRVRECRDGPRT
                     LDLDLLIYNDFECNDSSLTLPHPRMHQRAFVIQPLMEIAPDVRIPGQPALAELLAACG
                     GQRLERLKNNRE"
     gene            29806..30447
                     /locus_tag="VFI43_08975"
     CDS             29806..30447
                     /locus_tag="VFI43_08975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET7062295.1"
                     /translation="MKLEEYRHIVIEGPIGVGKTSLARRMANHLRGELLLEKPDDNPF
                     LDRFYEDMPRYALPTQLFFLFQRADQLKGLVQADMFARPVVSDFMLEKDPLFARLTLT
                     DAEYDLYHKIFRHLQPKAPPPGLVVYLQAPPGVLIERVKRRGNMFERSISTEYLQRLS
                     DSYMRFFHQYEAAPLMIINSENLNFADSEADFDLLLQQIEKMRGHREYFNRGI"
     gene            30477..31268
                     /gene="panB"
                     /locus_tag="VFI43_08980"
     CDS             30477..31268
                     /gene="panB"
                     /locus_tag="VFI43_08980"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7062296.1"
                     /translation="MRTTHDVFQKMRDDGEKIAILTCYDSSFATLLENAGVDILLVGD
                     SLGNVLQGEESTLPVTMDDMVYHTRCVARGSSKAFIMADMPFGSSQVSPEDTFENAAE
                     LMAVGAGMVKIEGGSVMAETVEFLTRRGIPVCAHIGLTPQSVHQLGGYKLQGRTDDQA
                     KQLLDDAAALESAGAAMLLMEVVPALLAKQVTEKLSIPTIGIGAGKDCSAQVLVLYDM
                     LGIYSGRKARFMKNFLAGGGSIHEAVTDYVRAVKAGEFPGPEHAF"
     gene            31281..32141
                     /gene="panC"
                     /locus_tag="VFI43_08985"
     CDS             31281..32141
                     /gene="panC"
                     /locus_tag="VFI43_08985"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7062297.1"
                     /translation="MEVITDIASLRQRLKYEPSIAFVPTMGNLHAGHLSLVRTARQKA
                     QCAVVSVFVNRLQFGPKEDFGRYPRTLAEDCALLENEGVDVVFAPGETELYPVAQEFQ
                     VLTPPMADTLEGAFRPGFFQGVSTVVLKLLNIVQPAVVVFGKKDYQQLVLMRQLVREF
                     NLPLEIVAGETERAADGLALSSRNSYLTLEERAEATRLYQVLSHIKQQMESGAGNPRE
                     LEADAKQNLQDHGWQVDYVTICDRCTLARAGARDGNLVVLGAARLGKTRLIDNLEISM
                     EGCGDVRKKR"
     gene            32259..32798
                     /locus_tag="VFI43_08990"
     CDS             32259..32798
                     /locus_tag="VFI43_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET7062298.1"
                     /translation="MAELIAVGFQDPEEADRVLLRLSKLKKEYLIDLEDAVVVVRDEE
                     GKVHLKQSINLVSAGATSGFLTGSMMGALVGMLFLNPLAGFVVGSAFGAGAGALSGSL
                     SDYGINDNFIKSVAETIPRNSSALFILVRKAQPERVLAELSGVKGKILHTSLSPEQEK
                     KLQEALEGSKPGSQLAPGQ"
     gene            complement(33028..33510)
                     /locus_tag="VFI43_08995"
     CDS             complement(33028..33510)
                     /locus_tag="VFI43_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7062299.1"
                     /translation="MAAAVYGASEGLNTLVLEQTAPGGQAGGTSKIENYLGFPTGVSG
                     EELADRAVLQARRFGAEISTPSRVTTLEFDEAYPVLHLDNGERVFARCLLIATGADYR
                     KLDVENREKFDGAGVYYSATHTDWLLPHIATDNKGFVKTGHVDCKFTPMDIEPSAVLA
                     "
     gene            34292..35059
                     /locus_tag="VFI43_09000"
     CDS             34292..35059
                     /locus_tag="VFI43_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7062300.1"
                     /translation="MFSLHSPKQNRILAALPAQDYARLLPFMEFVPMPLGQTIYEPNS
                     AVTHLYFPTTSIVAPIYAVENGASVRLAIIGNEGMTGTCFLLGGGGTPSGVVVQSDGC
                     GYRIKANTVKKEFETGGSLQHAILRYTQALLTQTAQNAVCNRHHKIEQQLCRFLLMSL
                     DRLPTEEMRMTHEQIAIMLGVRRESVTQAALKLQTINAIRYSRGHITVLDREELENAV
                     CECYEVVNDEYDRLLSNRSNMHSTSRQVSGKELWSVS"
     gene            complement(35159..37825)
                     /locus_tag="VFI43_09005"
     CDS             complement(35159..37825)
                     /locus_tag="VFI43_09005"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET7062301.1"
                     /translation="MPSAPRLDEIPFVVSRILLQDFTGVPLLVDLAAMRSVAQRMGRD
                     PKLVEPLVPVDLVVDHSVQVDHYGDKQALAINMEMEFRRNNERYEFIKWGMQAFDTFK
                     VVPPGIGIVHQVNLEYLARGVHVKEGIVYPDTLVGTDSHTTMINGIGVVGWGVGGIEA
                     EAGMLGQPVYFLTPDVVGVNLMGHLRSGVTATDLVLAVTQMLRKAHVVGKFVEFFGEG
                     TASLTVPDRATIANMSPEYGATMGFFPVDDKTIDYFKGTGRTDKEIGAFQTYFKAQNL
                     YGMPRKGDIDYTRELELDLATVTPSLAGPRRPQDRIEMGSIKAKFNELLHKPVNEGGY
                     GKDAEKLEQRYPVASCNETEPENLSQEPELALIDASLECEDAAPEPVAHVPPGSARNV
                     MEMVNNRPLMQKDEPPQCVSNGAFDLGNGDVLIAAITSCTNTSNPSVLLGAGLLAKKA
                     VEKGLSVKHHIKTSLAPGSRVVTEYLTATGLMPYLEKLGFTLAGYGCTTCIGNSGPLS
                     DPIEEALAANDLVCAAVLSGNRNFEARIHPSIRANFLASPPLVVAYAIAGTMLTDLTA
                     EPLGIGEDGKPVWLRDIWPSDEEIGALIKYAADGEIFRRLYSNLTEDHVLWNTVPSVS
                     GDVYSWPESTYIAEPPFFQDFSMQPSQPVGIREARALAIFGDSVTTDHISPAGSIKDT
                     SPAGRYLLEKGVSKSNFNSYGSRRGNHEVMMRGTFANVRIKNLMIPGSEGGITLHQPD
                     GERMSIYDAAMKYIADGVPTVVFAGEEYGTGSSRDWAAKGTQLLGVRAVIAKSFERIH
                     RSNLVGMGVLPLQFKDTDDVQSLGIKGDEQFDLLGLKDIRPQQDVTLVIHRADGSRSE
                     VDLLCRIDTATEIDYYMNGGILPYVLRELMAS"
BASE COUNT         8738 a        10741 c        10366 g         7989 t
ORIGIN      
        1 gaccatttgc atcacagcgc cgcccactga ccaggcattc cacgcaaata cgccgagaac
       61 tacaaaaccc aggtgactga tgctcgtata ggcgacgagc cgtttgaaat cggtctgcgc
      121 aaaagccagg agtgcgccat aaagcacacc ggccgccccc aaccccattg cgacgggcgc
      181 aaattgcagc gcagactctg gaaacaatgg gacaacaaag cgcaaaagtc cgtaggcgcc
      241 tgttttcagc aacacgcccg caagaaccac gctccctccc gtcggggcct cggtgtgagc
      301 atcgggcagc caagtatgaa acggcacgac ggggagcttg actgcgaaag caatgaaaaa
      361 gccgagcatg atccagaatg atgccgagtc cgacatgacg acattcagca gcgggaaata
      421 atcgaaggtg agattgccgc tgctccggta atgaatgaat accagcgcca ggatagcgag
      481 cagcatcagc agcccgctcc cctgcgtgaa aatgaaaaat ttgatggatg catagccgcg
      541 cctttcatgt ccccagatag cgatcaggaa atacatgggc accagcatca gctcccaaaa
      601 gaagaaaaag agaaacagat cgagtgcaag aaacacgccg acggcgcccg ccagcgtcca
      661 caagagattg aaataaaaga aacctgcccg ttcctggatc tcggtccatg aacacaagac
      721 agcaacaatg ccgagaaacg cggtcagaac gatcagcacc aggctcatgc catccatggc
      781 gagatgaaag ctgatgccaa agcgtggaat ccattcggcc acaaactccg cgagccaact
      841 gcctccctca agagtgattt tttcagaatg ttgcagccag agctggacag cgagaacaag
      901 ttctattgcc gccgtgatca atgctatcca gcgtgcagac ccggcttttc cgcgctcagc
      961 caccccggcg agcaacccgc cggccgttag aacgaatatc agcacagcga gaatcatatg
     1021 aaaatcgcaa tgaaaacaat ggccgctgaa ccggctgcga tggcggcagc atactgacgc
     1081 accaggcctg tctgggtggc gctcaagccg cgatagaaag ccaggcagag tcttgccacg
     1141 ccactgtaga cgttgtcgat cacatcgccc tggttgcttc gagcgagcca gatatatggc
     1201 cgcacgaata aaacgtcata aagcctgtcg aaatcccatc cgtaatgcca gaacttacgg
     1261 agtcttgcga gcgccggcat gagcatgagc ccatggagaa aagccggata cctgagaaag
     1321 agcaggtatg ccaaaaagat accggccagc gaagccgata ttgcgagcaa ctccaggatc
     1381 agctcggttc ttccgctttc atcgccatgg atgaactggg tggcaggcaa agcatgccga
     1441 acaaaattgg aaaacagggt cagattacca agaaaaggcg gagtttcaat ccacccgccg
     1501 gcgatcgaca gcgcggcgag gatgagcatg ggaacgctca tgcgcaaccc cgttcgtttg
     1561 gaaagttccg gaccccttgc ttcgccaaag aagacgagaa atatgacccg gaaaatatag
     1621 aggctggtca gtatggcgcc gacccagcct gcggcccaca accacgggcc cccattggtg
     1681 gacaaccagg attgagtcaa tatgaggtcc ttgctgtaga accccgccgt gacgacgggc
     1741 actgctgcaa gcgatgcccc gccgatcaaa aatgtccaga aggcgaccgg aagcttttgg
     1801 cgaagtcctc ccatgcggaa gatatcgtgt tcctcatgga ggctcatgat gactgcccca
     1861 gcgctcagga acagcagcgc cttgaagaag gcgtgggtca tcaggtgaaa gatggcggcc
     1921 gaccaggcgc ctaccccaag ggcaagaaac atgtaaccga tctggctgat ggtggagtaa
     1981 gcaagcacgc gcttgatgtc cttctgcgcc aacgcactgc atcctgcaag cagcagggta
     2041 agcgcgccga tcactgcgac ggcgaactgc accgatggcg caagggaaaa aaggacgtgg
     2101 gtgcgcgcaa tcagatagac acccgcagtc accatcgtcg ccgcgtggat cagtgcgctg
     2161 accggcgtag ggccggccat tgcatcaggc agccaggttt ggagcgggag ctgtgcagat
     2221 ttcccgaccg ccccccccag caacagggca gcggcagcag cggcaatccc cgagcctggc
     2281 ggaaaaatga cagctgcccg ctgcatgagc ggctggatgt caagggtgcc gagatgggta
     2341 aacagcagga aaagcccaat ggccaaggcg gtatcgccca cccgggtcac gatgaatgcc
     2401 ttgcgcgctg cctgcccgtt ggcagggtct ttataccaga acccgatgag aagataactg
     2461 cacaagccca ctccttccca cccaagataa agaagcacga gattgtcagc cagcaccagg
     2521 acgcacatcg agccgacgaa aagattcatg taggcgaaga agcgcgcgta accttcgtct
     2581 ccttccatga acccagctga atagacgtgg atcaggaagc tgataatggt gacgacgagg
     2641 atcatcacca gtgatagggg atcgaggtaa aaagcgattt ccggcgtgaa tccggctaga
     2701 tccatccacc tccatgcagt ttgccggaaa gcgaacgcag gcggaggatc aaggatgaag
     2761 ccggaggcaa tcagtatcgc gataagtgcg gcgaggccga cggagcccgc tccgaccact
     2821 gcaacgccgc ggtgaccaag gcgttctccg cccagcgcca gcgccatgaa accggcaaaa
     2881 ggcaaagcgg gaacaagcca caacagcgcc agcatctcat cctcgcattt tgttcaccgc
     2941 gtcggcatcg agcgtctgaa atccgtgatg caacagcagc agcagcgcca gaccgacaga
     3001 aacttcggcc gctgccaagg ccagtatgaa tatgaacatc acttggccgt cggcctgccc
     3061 ccagcgagcg cccgccacga tgaaagcgag ccccgccgcg ttgagcatga tttcgatcga
     3121 catcaggatg aaaaccaggt tgcgtcttac caggataccg atcatcccca gcacgaaaag
     3181 cacagccgcg agcaggagcc cgtgctgcat cgggatggca gcaatcgctt caatcgtctt
     3241 catgggctcg taactctccc cgcgccatcc cccatgagcc tctccgcagg ctctcatccg
     3301 acccctcttt ctcgcccgca ttcagacgca tgccctggtg ccggccaaga tggcacgccc
     3361 cgatcagccc cgccagcagc agtaacgacg ccagttcaac gccgagcaga tacggcccga
     3421 ataaggccat gccgacctcc tttgactcga ccaccgcggc gccggagctt gtggcgcccc
     3481 cgcgcgctag aatatagatc agttcagcca aaagtattgc cgccagtaca gcaggtccaa
     3541 tccacattcc cgggctgagc cattgatgtt cccgcgcctc ggccgctttt cccaagttga
     3601 gcatcatcac cacgaacacg aatagcacca tgattgcccc agcgtaaatg atgatttcga
     3661 gggctgcggc aaacggtgct ccgagtacgt aaaaaacgac tgcaagggcg agcagggata
     3721 cgatcaggta gagcagcgcg tgcaccgcgc tcgcccgcgt gatgaccatt gtggtggcag
     3781 caatggcaac agctccggat aaataaaatg cagcttccat ggcactccag gagcgggctt
     3841 caaggcaaca gcgaacgcgt atcgacaggc ggggcttcac gctccgcctc tcccttgccc
     3901 ttccctccta ttgcaactcc tgacgttcgg taaaagttat agccaggata tttgccggtg
     3961 ccgtcgatca ggaggtctcc tttttcatac accaggtttt tcctttcata ctctcccatc
     4021 tcgaaatcag gtgtaagctg gatcgcatcc gtagggcacg cctcttcgca ataaccgcaa
     4081 aaaatgcagc gcgaaaagtt gatacggaaa aattccggaa agcgccgccc gtgttcatct
     4141 tcggtcgcct ggagagctat gcaatccaca gggcacgcgg ccgcgcaaag ataacaggca
     4201 acgcagcgct cctgcccgtc ggggtcccgg ctcaggataa tgcgcccgcg ccatcgcggc
     4261 ggcatatagg gtttctcgtc cggatactgg atggtcaccc tgggccggaa cgtatgcagg
     4321 aagatgtccc ataacgtttt aagcgagttc cacatttcat ccctccttgt tcggatcaag
     4381 agcgggccag caccacagct ccggtgacca gcaggttgac cagcgtgagc ggcagcatga
     4441 ccttccatcc gtaggccatc aactggtcgt agcgtggccg gggcagggat gcgcgcaaca
     4501 aaacgaagaa cacgatgaca acaaaggtct tgagcaggaa ccatgccagc ggtggaagca
     4561 ccggtcccag ccagccgccg aagaacaagg tcaccaccat cgccgagata agcgtgacgc
     4621 cgacatattc gccaatgaaa aacaggccga atttcatgct cgaatattcc gtatgaaaac
     4681 ccgccaccag ctcgttctcg gcctcgacga gatcaaaggg caggcgtcgc gcttcagcga
     4741 ttcccgctat ggtaaaggtc agcagaccga gtacctgcgg aacgcaaaac cagaggtttt
     4801 cctgcgcaat aacgatatcc cggaggttga atgaaccggc gagcatgacc acacccatca
     4861 gggccaagcc catgaaaacc tcgtaactca gcatttgcgc cgccgcacgg aagcccccaa
     4921 gcagagagta tttgctgttc gatgcccaac cgccaagtac aatgctgtag acaccgagcg
     4981 aagacatagc caggaagaac aacaggccaa tgttcagatc gaccaccccg atcccgggtg
     5041 cgatgggcac cactgcgaag gcgagcatgg tagcggctgc gatgatggtc ggcgccagca
     5101 cgaacacagc cctgtctgca aacggtggta cccagtcctc cttggtaaga agcttgattg
     5161 catcagcggc ggcctgcagc gttccgaatg gccctacccg gttaggccca tagcgatcct
     5221 gccagaggcc cagcaggcgg cgttcgaccc aggagagcag cgcggaaaag gtaaaaagca
     5281 cggccaggat gaggataagg tttatccaga tacccacagt ttcattcatg tccagcgcct
     5341 cgcccgttgc ttcgcttctc tgcctttgtc accttggccc atgccggcaa ctccactcca
     5401 aggagatcgc ccaggcctgg taaaccgacc ggtaacccgg cgatcccttc gggaagttcg
     5461 ggtttgatct ttacaggcag ctgggcggta agcattttcc ccggtttgtc gattaattga
     5521 actgccacac tgtctcccgg cacgagccca agcccagccg cgctgccagg attgagtgcg
     5581 agatacggct gggggctcag ttccgagata ccgcgcgaca gcgtgctcaa ctcctctccg
     5641 ccaaatatat gatgcagcgg cactacgagc cacttatccg gcatcgaatg gaactccgcc
     5701 gggatatctt caaagtagtc cacttctccc tgcgccgctg gctcaatgag gcgttcaccg
     5761 ggattgccac ctctaagcgc tcccccaact tcctgctgga atttgttcaa tgactggatg
     5821 gaattccagc cgggggacca gaaacgaggg atcaacgaag gcggcggatc cccgccatag
     5881 ccttccattg aaaatgccaa aggtgtatcg ggatcgcgcg gctgctccgg ctcgtgcacg
     5941 ctgacgccag cgcgcattgc cgtgcgcccg ctatagcggt ggggctggcg cggtatcttc
     6001 tggccatcaa tgcggaagct tgcgggcggg gcagcatgca cgatgcgatc ccaccccgga
     6061 attgccaggg cgcaggccgc tgtaacatcg tcgagattac gccagtttgc ttcactcccg
     6121 cggcctgcca cggccataat atcgcgtatc catcgccagc tctcctggat ttcgccttcc
     6181 ggcacgaata cctggaagta gcgctgcgcg cggccttcgt tgttgatcag ggtgccgtct
     6241 gcttcggcga aggcggctgc gggaagcagc atatgagcct tttcactggt gcgatgggag
     6301 gtatgatcga taaccacgat atgccgggca gcagccagca tgcgttcgac cagcaccttg
     6361 tcgctgcgcc ggtacaggtc attttccagg atgacgagga tgtcggctgc atcgctttcg
     6421 agtaactcca gtgccgcttc caggtcgccg cccccctgca ttgccatgcc caggctgttg
     6481 cattcgggta ccgcgtagca gagtgctgca ttgcggccgg tattgcagag tgcccgtgcc
     6541 acattggccg ctgcctcgat gatgccaatg tcgccgcaac ccatcccgga gatgatcagg
     6601 ggatgttccg ctcccttcag tttttccgcg atagcattcg ccactgcccc cgcctcagca
     6661 gatagtcccg cgaccggggg tacgtgcgga tcgagcctat tagcgatcgc gaacccgagg
     6721 cgagtgatat cagcaggtgc tccgcggaaa gtctgagttg cgatatcgtc caggcgggtg
     6781 tccgctacgg tggcgacaag cagcggtcca tgctcgtgcc gtgtagcctc gcggacagca
     6841 ctatcgttcc agagcgggat cttcattctc tccgcccgct tttccggctg ccgtcgtact
     6901 gactgccgca atgagagtgc aagccgcgga gcggtattgc tgacatcctc gccaaggatg
     6961 aggacagcat cggcaagttc gacgtcgtgc agggatggga tacgggcagg cccctgcctc
     7021 aaaatatcca ggattgaggt cagtagccgc gattcccgtt ggttcaggcc cgtatggaac
     7081 cggtccgacc caaccaaggt gcgaagcgca aagttggctt cgactgaagc acgaggagat
     7141 cctattccga ccaccctgcc ggattgagag agaatttcgc ctaaatactc aaccgcatcc
     7201 gatccagcga ccggttgagg cagttcattt ttatccccgc gtatcgctgg ctgcctgatc
     7261 cgcaactcgc tgttaacgaa ctcgtaacca tagcgtcccc ggtcacagag gaaatacccg
     7321 ttcacgtcgc cgttgaaacg attgcggatg cgccgcaatg tgccgtagcg ctcgccggga
     7381 atggtgttgc aacccgagcc gcaatgcaca cagaccgagg gtgcggtctg aaggtcccac
     7441 ttgcgcgtgt aatgccacct gagtgtcttg tcagtgaaaa cgcccgtggg acacacttca
     7501 accagattcc cgctgaactc gctctccagc gtaccgtcct ggtgccggcc gaagtagacc
     7561 ttgtcgcgta gaaccagggc atccaggtcg cgtccgcctg cgtaatcccg atagaaccgg
     7621 acgcagcgat agcattggat gcagcggttc atctcgtgat tgaccaaagg cccgaggtcc
     7681 tggttatgaa aagtgcgctt tggtccgcgg taacggcgat atacatggcc gttcatgacc
     7741 gtcatgtcct gtagatggca ttccccaccc tcgtcgcaca ccgggcaatc atgcggatgg
     7801 ttcaccatca gccattcagc cacgctttcc cgaaactcgt gagcctcggc gtcatcgatc
     7861 gagatgaaag tcccgtcctt cgcaggcgtc atgcaggcca tcaccaactt gccccgcctg
     7921 tcctccgcgt ttttgaactg ttttacagca cactggcggc aggcgccaac cgaacccatg
     7981 gcgggatgcc agcagaaata aggcaggttg aatcctagcg acaggcattg ctgcaggagg
     8041 ttttcctggt cgtttgcagg ataaggccgg ttatcgatat ggatggttgc cacgttcgcc
     8101 tctcatgtat ccgtcagttt tctctccagg ggcaacgttt ttcatgaatg tggcgctcga
     8161 aatcgtcctt gaaatatttc aggccgcttt gcagcggctc agcggcgccg ggggcaagcg
     8221 cgcagaaggt atgcccggga ccgagcagcc gcgtgtggga cgcgaggcgc tcaagatcgg
     8281 agggctgccc gtggccttct tccatgtccc gcaaaatttg ctcgatccat gaaagtcctg
     8341 accagcatgg ggtgcaccag ccgcatgatt cctgggcgaa gaaatgctcg agattgagaa
     8401 gcattccgac cgggcaggtc ctgtcgtcga gaatgatcat tgtccccgtc ccaagacggc
     8461 tgcccgctgc ctgcatggaa tcaaaatcca tgggtacatc gagatgatcg gcaaccacga
     8521 agtccgtgga ggcgccaccg ggcaaaagac cgcggaactg gtacccatcc tgcatcccgc
     8581 ctgcatgctc ctccaggatt tcccgtatgg gcgtgcccat cggcaattcc cataacccag
     8641 gctttttcac ctttccactt gccccataca gcttggtgcc gccatccgcg gtatggctca
     8701 ggcttttgaa ccattccgcc cccttgtcga cgatatgcgg gacgttgcat aacgtttcaa
     8761 cgttattgac aatggtgggc tttccccata ggccgctcac ctgcggatag gggggcttgg
     8821 cccggggatt ggcgcgcctg ccttcgagcg cgttgagcag cgccgtctct tcgccgcata
     8881 tatagcggcc cgcactggca tgaagatgca attccagact gtatcccgat ccaaggatat
     8941 tttttccaag ataacccttg ccataagcct caccgatagc gcgctccatc ctatgtgccg
     9001 ccagtttgta ggcccagcgc aagaagacaa aagcaatgtc tgcctggagg gcataagcgc
     9061 tcacgatcat cccctccagc aactggtggg gattaccctc cagcaataac cggtccttga
     9121 acgtgccggg ctccatctcg tccgcattgc agacaagata tttcggacgc ggagcatcat
     9181 cgcccatggg aacaaaactc catttctggg cagtgggaaa tccggcgccg ccgcgacctc
     9241 gcaggttggc gtccttcacc acttgctgaa cgtcggcggg agccatccgc aaggctttcc
     9301 gcaacccgga ataacccccg gttttctcat attgagccag gtccggaggt tctttccctg
     9361 ttgaaatgtc atgcgtcagt ggcttttcca tccgcctgcc cgccttcttt tagttcgcag
     9421 gaagcaagaa tctcgtcgat tctcgccgga tccagatcct ggtaatgcgc atcatccacc
     9481 atcattgccg gggcgtggtc gcatgcgccg aggcacacgt tcggcaaaag cgtaaagcgt
     9541 ccatcggcgg tggtctgccc gaactcgatc cccaggcgcc gggtgaggtg gtcgcgaagc
     9601 ttctcataac ccataatcca gcaactgacg ctgtcgcata gcagaatgac gtgcttgccc
     9661 accggttggc ggaaaaccat gttatagaac gtcgccaccc cttccagctc cgcgagtgtc
     9721 atttgcaacg cctcggccac atcggccagg ccttcatccg atacccagcc cctatgacgc
     9781 tggatgatct ggagcgcctc gatacagaca gcgcggcggt cggggtaatg ccgcgaatgt
     9841 gcttcaattt cctctctttc ctgttcgctt aacatagatt cacctagcga tccacatcgg
     9901 ccatcacgaa atcgatgctg cccaggatgg cgatcaaatc ggaaaccatc agccctcggc
     9961 ttatctgcgg gatcatttgc aggtgcggaa aggacggcgt acggatgcgt acccggtatg
    10021 ccatcgtatt gccgtcgcta atgaggtaat acccattgtt gcccttggtc gcctcgatgg
    10081 ccatcaatgc ttctcctggc ggaatgactg ggccccagct tacgcccagg aaatggtcga
    10141 tgagcgtctc gatatcgtga agcgtgcgct tcttgagggg aggcgttgtg agcggatgat
    10201 ccgccttata ggttccccct ggcatattct caagacactg cccgataatg cgcaggcttt
    10261 ggcgaatctc ttcggcgcga accgcgcagc ggtcgtaaca atcgccgtga tctccagtag
    10321 gaatgtcaaa ctcgaaattc tcatagcccg agtaaggcct cgccttgcgg aaatcccact
    10381 cgaaaccgca tgcccgcagg ttaggaccag tcactcccca ctcgattgcc tccttaatgg
    10441 agtagctgcc aatgccctgg gttctcgcct tcacgatccg gtttccgaga gcgatctcgt
    10501 catactcgcg caatctggcg ggcatatagt caagaaaatc ccttaccagc cgatcccatc
    10561 ccgcaggcag atcctgtgct actccaccga tccgatacca gctcggatgc atgcggccgc
    10621 cgcagatcgc ttccacgatg tcgaatatgc gctcccggtc agtgaacatg aagaacacgg
    10681 gagagagctg gccgaggtcc tgtgtaaaag ttccataaaa cacgagatgg ctggcaatgc
    10741 gaaaaaattc ggccatcatt acccgtataa cttttacccg ctcgggcacc tcgatgcctg
    10801 ccagtttttc cacggcgagc acgtatggga ggttgttcat gacgccgccg aggtaatcga
    10861 cacggtcggt ataggggatg taggtatgcc aggactggcg ttcacccatc ttttcggccc
    10921 cgcggtggtg gaatccgatt tcgggcaccg cgtcgacgat ctcctcgccg tccagttgca
    10981 ggacgatacg aaatacccca tggacgcctg gatggttggg tcccaggttg aggaacatga
    11041 agtccgtccc atcatgtgcc cggcgcatcc cccattcctc gggatgaaac ctcagcgcct
    11101 cctgctcggc ttcctccctt tggggggaca tatggaaggg ctccatctcg gtcgcacggg
    11161 ccggataatc cttgtgcaaa gggtggccct gccagccagg gggaaggaga atgcggcgaa
    11221 gatggggatg accatcgaag acgaccccga acatatccca cacctcgcgc tcgtaccagt
    11281 tggcagctgg ccagagtgct gtaatggtag gagtcgtaag atgcgggcta ttcagcgcaa
    11341 ccttgaggcg aacatatgca ttgcgctcga acgagaagag gtgataaacg actgtaaagt
    11401 cgctttccgg ctgcccctcg cgatgccctc gcgtgcgttc atcaatcgcc gtaaggtcgt
    11461 acaacatgcg gtagggcttt tcggcctctt ctttcagata gcgaagaacc cgaggaatgc
    11521 tgctttttga aatccagcac gtgggaactg cgtcgcgtgt ctgctgaaag gggccgattg
    11581 cttccccccg agttgcggag ggtccgaagc gagcctcgat ctcttcgaga atgtcaggaa
    11641 aaccgggttt caacgcaaga ctggaagaag catgggtcac gcgtcatacc tcatccggag
    11701 ggcgcagcgt ctcggcgctc actctcgcag cccggttcag gtctcgctgc gacggccgcc
    11761 ccggcttcgc gatcccctgc ggccccacca cccagctcag gggacgcttc tcctttccga
    11821 tcgcatcctg aagaaggttc aatccctgaa tgaatgcatc gggccgtgga ggacaaccgg
    11881 gaacgtagac atccacgggc aggaatttgt cgaccccctg gaccacgctg tagatatcgt
    11941 acattccacc ggagttggcg caggaaccca tggagatgac ccagcgcggt tccagcatct
    12001 gatcgtagag gcgttgtatg acgggagcca tcttgatgaa aacagtaccc gagattacga
    12061 tcaggtcggc ctgccgcgga gaagcgcgta tcacttcagc tccgaaccgc gccacatcgt
    12121 atttactggt gaagctcgtg gccatctcca cgtagcagca ggacaacccg aagttatagg
    12181 gccaaatcga gtttttacgt ccccatgcga caagatcctg gagccgtgcc aggagaacgc
    12241 ttctgcggcc atcgtccgaa aaattcctga tatcgttaac cgcgtcttct gtcgattgtg
    12301 tacctgcttg tgccggcttt cgagaatccg acctttcatt tgtcctggtt aacgaccatt
    12361 tcatggtttg attccctttt tcagttcctg tctcccggcc acgcgcgaac gccgccagtt
    12421 cctcccatcc tctggcgtgc cccaatccag cgcacccaac ctccacagat aagccagtgc
    12481 agcgcccagg acagcgataa atatgagtgc ctcgacatat ccggcccaac ccagctcccg
    12541 aaaagcgata gcccaggcaa aaagaaacgc ggcttcgaca tcgaaaatta caaagaacac
    12601 cgcgatcaga taaaatttgg cagataaccg caggcgtgcc aagcctgcgg ggacgattcc
    12661 gctttcgaac ggttcgtttg tttccgggct cttgtggcgt tccccgatta cgtaggaaag
    12721 tcccacaatg gtcgcgagaa gcatcacgac aaggaagaaa taggcgacca gcggccagat
    12781 ttcaaccggc gagccctgaa cggtattcat ggggggaagg gtttcctctg ggcctggttc
    12841 cttgtcggcc tgccatgccc gattcctgta agggaagcaa acgcaagtat ttcaaaccag
    12901 aatcgcgtgt tctgtgcgat agacaacagt gggcaggtgc aaaattacct ccccaatccc
    12961 gctggaatgc ctcgaatggc agcaaattgc ctgggatgat cttcaaagat atgcatgcaa
    13021 catgagaacg accaagcgga gggggagaac gataaaggcg gatataagcg ggcaacgaga
    13081 cctgttttct gtctgctcgt tactgctgaa agtgattgtg gcgctggcga tcctcgtagt
    13141 gaatactatt gcggctgctt cggcggatac gttccgcaac agttgcgaaa agacggtcgg
    13201 cgcagggaag gtagtggtta catatatcga cagcgaaacc atatacaaca gctcacattc
    13261 gacaaaatcg ctccaatccc ggactgccaa aagcagcgac agctatcaca gcatttatgg
    13321 attgacacac gcggaaccat cattccgcct ggaaatacgg tcacggttgc tggtgaacgc
    13381 ggatggacag acgtgcatgg ttccggatgt ttcagtaaaa ctgggctttt cggcaatgca
    13441 cgtatacctg gccaaagagc tcggggacag ctgcaggaaa aatatcgtgc gggagcacga
    13501 actggagcac gtatccacgt ggaaatccca ttttcgcata ggagcgaaaa tgctcaagga
    13561 accgctacgg gcggcattct cccgaccccg tcattacagc tcggagaaag aagcgcaggc
    13621 cgacttgaaa ccctgggtgg aagaagtgct gaagcccttg cagcataaac tggtgaattc
    13681 agtgatggac gctcaacggg cgatcgatgc tcctgcgtct tatgagcgag taaggcagcg
    13741 cctgtatatg tgccttgcag gaaacagctg actttgcttc aatatcggga taatattcaa
    13801 aagctcaggg acccagagac aagtcccggc gggaggtggc gtgcaggatg tcgtgcgtga
    13861 aaggaagagc gggttatttc cgctttaagg cttgggacac cctatgcatc cgttcgtatt
    13921 gagcttggaa aaatcgactt catcgaggac tgctccccgt aggtcagctc cctgaagagt
    13981 tgcattatca agctttacgc ccgtcatgtt ggctccgcgg aagtcggctt gtgtcattac
    14041 gctccctgat aggtccgccc tttgaagctg agcattgcga aacttcgcat tcgtcaggtt
    14101 cgccccgctc aaattagcgc ctttcaattg tgcatccgtg agatcggatc gcgtcaagtc
    14161 agcgctcttg aggcgcaccc cgttcaactg ggcgtgatcg agcctggcac cgacaatcgt
    14221 tgcgtttttt aaatctgcat cgttcagtac cgcgccttca agatttgaac ccgtcagatt
    14281 gaccatatcc atattcgcgt tcagaagatt ggccccctgc aagttggccc cgctcaaatt
    14341 ggatttccga aaacctgccc gcgcgagatc agcgccatgc atgtcggctg ctcgtaaatc
    14401 cagttcgatg cagagcgcag cggggcggat ttcgcagcca ttgatattcg cggcaacggc
    14461 actatccgtg gtaattgcgg cggcgagcag aatcaggtaa cggttcattt ttcaccggga
    14521 tcgaggaata ataaatggat ggtatatgat tctctctgct gcatcggttt ggtaaccaac
    14581 atacccgaca taccttggac tgcacatctg cctggaaaaa aaaccaatag cggatcgagt
    14641 taactcgatc tttgatgcgg aatcaggaac ctttcattct tttcaagtcc tcgattactt
    14701 caacagaatt tcgcgacgcg ctcgccgata aatagatttt ggcgattctg ccgtccgggt
    14761 cgatgaggta agtgtttcgc ttggccatac cgatgatgct ccacatcgaa tcgtaacgct
    14821 tcgcggtctc gcccttgctg tccgccagaa gcggaaaggg aagattatat tttttggcaa
    14881 aaccggcatg gctggcgctg tcgtccacgc ttataccaac gacttccgcg cccagatccc
    14941 tcaattggtg aatgtcatca cggaacttgc acgcctgctt cgtgcaacca ggcgtgttgt
    15001 ccttgacata gaagtacaag gtcagccact tgccgtgata atcagagagt ttgtgggtct
    15061 tgccgttttg atcaggaaga ttaaaatccg gcgctgcctg gcccacctta agaggttcgg
    15121 caactgcacc acgactcccc aaagtcatga tcattgttac taatgctgca agtatggcga
    15181 acaatttcat gatttcatcc cttgatattt ccattaatct tgccaattcc gatcggataa
    15241 gcccatttgc agcaaaccct gccggatcga tcgtcaacat ttgtgcctgt tgacacttgt
    15301 ttcgcccccg cctccgtgac gagatttcaa ccggaacgag gcacgagtcg gacagcggag
    15361 caagctttgt aagcggcgag taacgatgga ccgacgaatt ccatccggtg cacccaaaaa
    15421 aagcgccgca atcacatgtt cctgctcttc ttgatcaggt cataagctgc ctgcacttcc
    15481 tgtgcttggg cagtcgctac tttgatcata tcctccggga gaccttggcc cattaattta
    15541 tcaggatgat actggctcat gagctttcta taggcacgct tgatttcctg gtcggtactc
    15601 tccctggtca cgcccaaagc cttgtacgct tcatcgaggg aggtagggga agcagctttg
    15661 cctcccgcaa agtgggactg gttcagaacc atgtccagca gttgccggaa tgcagattca
    15721 tcatagccga gatgcagcgc gcagtttctt aacagcgtct cctcccgggc gtccataagc
    15781 ccgtcggaaa ttgccatcac gatgagataa accagaagca tctggcgcag gctgttggta
    15841 tgtccgcaaa cgaccatgaa ttcgtccagc ttgcgctcgt aatcaaaatc gggggcggct
    15901 ccctgcttga acagcgcgat tgcctgttgc cggtgctcgg tcgtcattcc cagtttttgc
    15961 atgaaatctt ccacgtggga tatctcatcc ggggtaatgt gcccatctgc ctttgcaagc
    16021 tttcccatca tgatgaagac tgtttggatg aagacggcct gccgattggc gttggtcagg
    16081 ggattgactg cccctgcccc atacgcaaca tatctgtcga tgaagctgcc gacgatatag
    16141 ccgaccaagg cgccgaaaaa gccgaggaag tacaaaccca gcaaggctcc gattatcttg
    16201 aacaaggcaa tattcctgga atgggatgag atgggagtga atgagaaacg cagccagagg
    16261 catctggaat tatcccccaa ctagcgcccg aataatatta ataggtaata ttactattga
    16321 agacaggtaa ctcattctcg ccgctgctca ctgctggcag atggcagacg aaaaacagga
    16381 ggaaatgatg attgaattgt actcaagtcc taccccaaat ggccaaaagg tcatgatcat
    16441 gctcgaggaa accggcctgg agtggaagca tatcgacgtc aatatatcgc ttggggagca
    16501 gtttcaagcg gagcatctga ggctcaatcc caataacaag attcccgcaa ttatcgatac
    16561 ggatggaccg ggtggcagac cttacacaat gatggaatcg ggcgccattc ttgtttatct
    16621 cgccgaaaaa accggaaagt tcatgccgag ggatgagcgc aggcactacg atacgctgca
    16681 atggctgttc ttccaggctg cccatatcgg gccgatgttc ggccaggcga atcacttcaa
    16741 ccggcaggcc agcgacaacc actacgcgag ggaccgatac aacaatgagt cgctgcggct
    16801 gtatcgtgtg ctcgagaatc gcctgcggga gactgactgg atcgcgggag atgaatatac
    16861 gatagcggac atcgccacct acccatggtg caagggccat gcggatcgcg gcgtgaacaa
    16921 ggcaagcttt cccaatttca tgcgctggtt cgatgccatg gaatcgagac agggtgtgca
    16981 gcgcagcaat cgaatggcca ccgagattcg agagcgcatg gacaaggcgg cggaaggtcg
    17041 aaaacccatc gatatttatg acaccaagga taacggagat cgccttgctc gcgccactcg
    17101 cgactaggat ccctgctaac ccagcattca ggccacgcgg tgcaaggatg ctcggaagcg
    17161 gccgtcagct aacttgaaaa aaatcagcga taaaacaagg ctgcagcttc attctctggt
    17221 aaacaattgc tcaaaatttg ggtcaaaaaa gcagacggtc tgacaagtgc ctatctgaca
    17281 cgcggcgtcg ggcggattca tcctgtgatg caacccgcgt cggcaaggtc gctttccttc
    17341 cgtcactcgt tcacgaaatc atgcatcacg atctcgcagt gcccaggcac attaattcac
    17401 tggatataaa atatggcgtc tttcaagagg ttttgccttg cgatatccct ggctgctctt
    17461 ttagcagccc ctcaatttgg ctgcgccccc tacatggctg caaaacaacc aggggcaaaa
    17521 aacctggagt tgtttaaaac aggaactccc cgaaatgctc tattggccga gtttggcgcg
    17581 ccggcggtga gtgaaaatag aaatgggact caacacgaaa tattcaggtt tgtgcagggg
    17641 tacagtaccg cagcgcgaac aagcagggcg cttttgcatg gagtggcgga tgtggcaacc
    17701 tttgggcttt gggaggtcgt cggaacaccc gcagaagcag tatttagcgg ggacgaaatg
    17761 gcatacgacg tcacttatga caagaacgac cgcgtcgatc aggtcacgat actgaaaatg
    17821 gataagtaaa gcgggcagga acacaagttt ggcaggaaac gaaacttgtt cgaagctccc
    17881 ctttcatcct tttcccctgc caaggaattt atccatgggc atcgaccaag ccccatcgac
    17941 acagttttgc gagctgttgc aattatttgc cttacgtccc actaacgtac tctaaattcg
    18001 gaattctgca tactccccta atcgggcgct cccacgttgg gcgctgccaa cttccaccgt
    18061 ttggctgaag ctgcggatca ggcttgggct taaccagcct tgcgtagccg ctcgccaaag
    18121 cctgccaggc aaagccgctc agcagcatga cgcaggtctc gggttcgggg acgattttgc
    18181 tggataaacg aggatctgtc catgattatt gatcccgtac gcttcgtcta tagtgtttct
    18241 gcccggcagg ttcaccacca acgagttgag atcggttatc cccacgccat tggggcctgt
    18301 gatgaaggcg tgaacagaac catcggctgt gccagaactg ccgaccgcct gtccggcatc
    18361 attgatgcca gcggcgacac tttcactgta agtcccgcct aacgtaccga gatccctcat
    18421 tcccccgcca tcaggacccg tgatgaaggc gtggggaaaa ccatctcggg tgtaaaaatt
    18481 tccaactacc tgtcctgtgt cgttgatgcc agtggcggaa ctgtcactcc ccggcacccc
    18541 caggtctctc atccctacgc cattgggacc cgtgatgaag gcatgcttgg gatcactgcc
    18601 tgtgctggaa tatccgacta cttggccgga atcgttgatg ccagcggcag tactatgggt
    18661 cccgcccctc agggcaccca gatccctcat ccccacccca ttgggacctg tgatgaaagc
    18721 gtgggaagaa ccgtccatgt aagaatatcc gaccacctgc ccggcattat tgatgctggt
    18781 agcgtaactg ttactgtagt aatccctacc cagcgtgccc agatctctca tccccacgcc
    18841 attgggaccc gtgatgaagg catgggaaga aaaaccgtct tcggtatcag aatacccgac
    18901 cacctgtccc gcatcgttga tgccattggc gaaactcaaa cgcccaccca gcgtacccag
    18961 atccctcatc cccacgccat tgggacccgt gatgaaggcg tgaacagaac catcggacgt
    19021 gagaaaatgt ccgaccacct gtcccgaatc gttgataccg gaggcgacac tataagtccc
    19081 gcccaaggta ccgaggtaag tcagtttgcc gtcaggattc aagatggccg cagtgccagc
    19141 gaagaccggc gccacgtagc caaggcttgc gctcagcact gtcgcggcaa gaaaattgca
    19201 gactttagag cagcggatgg tttggatggc ggtgctccgt caggaacggc gttgaggagc
    19261 ctggttgttt gaatataggc gatcaaagcc aattgtcgaa agcgcccgga tgccgggaga
    19321 acatgccgga tagagctcgt aaactcccct atagccatgg atcaggccaa cttgagcagc
    19381 cagctttgtt aaggcgcgcg ctaaagctta tcaggaccaa accggccggc atggcacagg
    19441 gttcgggttc gggttcgggt tcgggttcgg gttcgggttc gggttcgggt tcgggttcgg
    19501 gttcggggga caatagtagt agtaaagaca agaatctgtc catgattatt gatcccgtat
    19561 gctaaggcta taaatttccc gcgctgcagg ctcacaacga gttggcggcg ctcatcccac
    19621 ccgccctgtc agccttccag cggcaggtta tcctgcagct ttcgcaactg ctcgatttag
    19681 aaatccgtat atccgccgtc tccatggggt aaacttgagc catcttttac tccagttttg
    19741 ttatggctca atatgttttg acaatgaacc gcgtgggcaa gatcgttcca cccaggcgca
    19801 cgattctcaa ggatatctcc atcagtttct ttcccggtgc gaagatcggc ctgctggggc
    19861 tcaatggctc aggcaagtcg acggttctga agatcatgtc gggcgttgac aaggatatcg
    19921 agggggaggt taccgccatg cctaacctca acatcggtta ccttccgcaa gaacctcaac
    19981 ttaatcccga acaaacggtg cgcgatgcag tacaggaagg actgggtgaa ctgtttacag
    20041 cccagcagaa actggaagag atctatgccg cttacgcgga gcccgatgcc gattttgacg
    20101 cgttggccac ggaacagagc cggctcgaag ccatactcgc caccggcggc ggaaatacga
    20161 gccaacagat ggaaattgcc gcggatgcgc tccgcctacc cgattgggat acccgggtca
    20221 aacatctttc cggaggggaa aagcgccgcg tggcattgtg caaactgctg ctatccaagc
    20281 ccgacatgct gttgctggac gaacccacca atcaccttga tgcagagtcc gtcgaatggc
    20341 tcgagcagtt tctcacgcgg tttcccggta ctgttgtggc agtcacccat gaccgttatt
    20401 ttcttgacaa cgctgccgag tggatcctgg agcttgaccg cggtcatggc ataccctgga
    20461 agggtaacta cagttcgtgg ctggagcaaa aggaaaaccg cctgaagcag gaagaatcgg
    20521 cggaatccgc tcgacagaaa tcgctgaaga aagaactgga gtgggtcagg cagaatccca
    20581 aggggcgcca agccaaatcg aaggcgcgca tcgcccggtt tgaagaactt aattcccagg
    20641 aatatcagaa gcgtaacgag acgcaggaaa tattcattcc cgtcgcggac aggctgggca
    20701 acgaagtcat cgaattcaaa aatgtctcga aagcctacga tgatcgcctt ctgatcgaga
    20761 atctaaactt caaggtaccc gctggcgcca ttgtcgggat tatcggccca aatggagcgg
    20821 gcaaatcgac gctgttccgc atgattacgg gaaaggagca accagattca ggtgaaatcc
    20881 gcgttggacc gacggtcagg atagcgcacg tggatcaatc ccgcgaagcg cttgatgggg
    20941 ccaaaaacgt tttcgacacc atttcggggg gccatgatat cctcaccgtc ggaaagtacg
    21001 aaacgcctgt gcgcgcctat cttggccgtt tcaacttcaa gggagcggat cagcaaaaaa
    21061 ttgtgggaaa cctgtccggg ggcgagaggg gccgacttca cctggcgaaa atgctcatct
    21121 cgggaggaaa cgtcctgctt cttgatgagc cttccaacga tctggacgtg gaaacactgc
    21181 gcgcactcga ggaggcgctg ctggaattcg ccggctccgt cctggtgata tcccacgacc
    21241 gatggtttct ggatcgtatc gctacccaca tcctgtcttt cgaaggaaat tcgcaggttg
    21301 cgttcttcga cgggaattac caggaatacg aagcggacaa acggaagcgt ctcggcgaag
    21361 agggcgcaaa gcccaagcgg ctacgctaca agccgatcaa ccgatgagcc gccatttatg
    21421 aaaccggcgg ctcaattccc gtggaaggga ccgaccttcc cgctaataac ggccctcgcg
    21481 cttcggacca gttccagtga tgtcggtatc cgagtcatca tagcgctccg tcccatccgt
    21541 actgtaccgg gagttatccc gttttttttg cgtggttgaa ccttccttta tatgcaacct
    21601 ctcttttatg cttttgtcct tattattggg gttcgtgtcc cgaacggctg gggaatccgc
    21661 cgggccaatc tcatcgggga tgtcctgccc tgctgaatga accaatccgg cgccggtcaa
    21721 acctaaaatc actgaactgg taacgacaaa cgatctgaag actaatctcc tggttttcat
    21781 gtttattctc ctgagttcgc tctgcgggat ttaagctatc ccaaatgata gcgtcgccgt
    21841 caagctgttc agtacgctag cacacttaag cacaggaata tttatccaga taacagactg
    21901 ctttcagctt gcgaacaaat gcaaaagagg acgcacgtgc gaggagagag cggttcgagg
    21961 gggcaagtta taaaaacggc gataaccccc gctcatgtcc tcggttgcga accatacgaa
    22021 ccaagctgtc tgatatcatg catgtgccat ccctgccgac gggcgagatg acgctctggt
    22081 tccttaacag ccctcgttca tgaaaacgat ctctttcgta ttcttattcg cttgtgtcct
    22141 taccctctta cccatatcaa ccgaggtgtc ggccttggaa caagaggaag cttgccccga
    22201 atgtcccgtg atggtgtccg ttcccggtgc taaatttgcc atcggaaaat atgaagtgac
    22261 tttcaaggaa tgggatgcct gtgtcgcaga tcggggttgc gggggctacc agccctatga
    22321 caacggctgg ggccgggacg cccggcccgt gatcaacgtg agctggaacg atgttcagac
    22381 atatattcgg tggctatcgc aaaaaaccgg caaggcctat cgccttccca ctgagcagga
    22441 atgggaaatt gccatgcggg gaggcacgac caccgagtat tattggggca acgatgtagg
    22501 ccgaaacaac gccaactgcg atggatgcgg gagcgaatgg gataacagga aaactgcgcc
    22561 ggtaggcagc ttcaaaccga atcccttcgg cttgtatgac atgatgggca acacatggga
    22621 gtggaccgat agttgctgga aaggcaattg catgaagcgc atgctttgcg gcggttcctg
    22681 gaaccacagg cctcaggata tgcgtgccgg catgtgcaac tggtttgaca cgaacaagcg
    22741 gatgcgctac ctggggtttc gagtcgccct ttcccttccc tgacttgcga tacctcgccg
    22801 ctccaccctt gctcaagcct ccacgttctg gcagaccgct agctggcccg cctgatactt
    22861 gcgcccagca gggatagctt gtgctcgata tgctcatatc cgcggtcaag atggtacacc
    22921 cggtcaataa ttgtttctcc cttggcaatc agccccgcaa gcacaagact tgccgatgcc
    22981 cgcaagtccg tagccatgac tttcgcccca tcgagcgctg aaacgccaca caccacagcg
    23041 gtgtttcctt ccaccttgat atcggcattc attcgttcaa gttcctgtac atgcatgaag
    23101 cggttttcaa atatggtttc agtaatgatc gcggtgccct ctgcaatact gttgagtacc
    23161 atgaactgcg cctgcatgtc tgtcggaaat gccggataag gtgaagttcg aaggttcacc
    23221 gacttgagcg gagtgctcat tcgcagatgt atccagtctt ccccggactc gatcgcagcg
    23281 cctgcctcca tcagtttgtc cagcacggca tcgagcgtat ccgttctggt gtcgagcaaa
    23341 cggacatctc ctccacttgc agcagcggcg acaaggaacg ttccagcctc gatccggtca
    23401 ggcattaccc tgtgccccgc cgaatgcaac gcagtaacgc cctcgatcgt gatgatatcg
    23461 gtgcccacac cttgaatttt agcgcccatc gcgatcaggc agttcgcaag atccgttacc
    23521 tcaggctccc gcgccgcatt ttcaagcact gttataccgt cggcaagggt agcggccatc
    23581 atcaggtttt ccgtcccggt caccgttacc agatccatga caatatgcgc gccgcttaag
    23641 cgtttcgcct tggcatgaat atagccatgc tcaataccaa tgtcagcgcc catcgcctgc
    23701 agtcccttta tgtgctggtc taccggacgt aaaccgatgg cgcacccccc cggcagggat
    23761 actctggcct cgcccgcccg ggcgagcatt gggccgagga ccaggattgc cgcccgcatg
    23821 gtcttgacca tttcataagg cgcgacaagg tgagacaggc gcgaagcggt aagctgggtc
    23881 cttttgccat ccactgcgac ctgaacgccc atttggcgaa gaaggctcag catcgtggtg
    23941 acatcattga gatgcgggac attatcaaca aagagtgtct cttcggtgag cagagaagcg
    24001 cacagaatcg gtagcgcggc gtttttcgcg ccggatatgc aaacttcgcc taacaggggg
    24061 ataccgcctt gaataatcag tttttgcatt ggaaacaggt tacggtgtca cgggagatgg
    24121 gtttcatgta cctcaagacg tactttcaag gcttcgaggc tgagctaagg catcaattaa
    24181 gggatcaagc ggcagaaatc attggggaga gggaggggca ccatcggtta gcccaaattc
    24241 ggaaaagcgt ggacaaccgt aacaaacggc gtgtcccggc tatacgaaag gtcctttcta
    24301 tgtcggacgg gtgactggag tgacgggatc agagtcgaca gcagtttagt gaaggttcat
    24361 gcgcgggacc atggttgaaa tatgcccgag ttcgcttcat catatcgcaa agtgcgcctt
    24421 aatggttttc aaattgcaat ccatcgctac ctacgacaat aatgaggaga tcagatgaag
    24481 aaaagtacgg tccagaccaa tgatgcgccc aagccgatag gtccttattc ccaggcaata
    24541 cgggtgagcg gtggagatac ggtttacctg tccggccaga tcggccttaa cccttctacc
    24601 atgcaaatgg ccccggatgt ggaagagcag gcgcagcaag tgttttcgaa tcttatggca
    24661 gtcgctgcag cgagcggggg aagcctgagc gatatcgtca agttgaatgt ttacttgacc
    24721 gatctttcca attttgaaaa agtgaatgcg atcatggcaa gccatttcaa ggagccatat
    24781 cctgcacggg cggcaatcgg ggtagcggcc ctgccgcggg gtgcgctggt ggaagttgac
    24841 ggggttctgg tattgccggg gtgaatggca gacgcaactt cgatcatggc ttcccctgct
    24901 caggccggat ctattggcgg agcgctggaa gataagctgt cgaggctagg catcaaggat
    24961 gaactcggcc tgctgatgca tttaccattg cgttatgaag atgaaacgca tctttatccc
    25021 attcgggatg ctcccgagaa caggatcgtg caggtcgagg gggtcattac ccacagccag
    25081 gtcatgtttc gcccgcgtcg gcagctcgta tgccgcgtgg atgacggtac cggcgtgctt
    25141 gtcatgcgct ttctcaattt ttacggcagc caggcaaagg cgtacgcatc aggggggcgc
    25201 gtccgcttgg tgggcgagat acgcggtggc ttttttggta tggagatggt tcacccgaaa
    25261 tgccgcctca taaatgggac cgagccattg cccgacacgc tgactccagt ctaccccacc
    25321 acggctgggc tttcatccag cgcgatccac aagcttgttc tgcgcgcgct caaacgcgta
    25381 cacggttaca gcagcgcagg aaatgagaag caggaggatt gcctttcaga aacactgccg
    25441 gagaatatcc tggaacggtt gggtttaccc ggtttcagaa atagcataac cttcctgcat
    25501 cagccttgcc ccgacacgcc tgtcgcatcg ttggaacggc gtacgcatcc ggcgtggcgg
    25561 cggatcaagt ttgacgaatt acttgcccag caattgtcca tgcgcctcca ttaccgccgc
    25621 aggcgcagcg gcagcgcacc cgttcttgag gcaaggaacc ggttggccgg caaactgctt
    25681 gaatcactgc ccttccaact cacgaagggg caaaagaaag cggttggcga gatcagcaag
    25741 gatctcgccg ccaccttccc catgcaacgg ctcctgcaag gcgatgtagg gagtggcaag
    25801 acagtggttg ccgcgctcgc catgctacag gccatcgaaa acggctatca ggcggccatg
    25861 atggcgccta cagagatttt ggcagagcag cattatcgaa aactgtccaa gtggcttctt
    25921 cccatcctgg aaccgctggg gatggcaatt gcgtggatat cgggtcagca gaaaaagagc
    25981 cagcgggaat cgatgctgaa tcatatcgcc agagggcgcg ccatgctggc catcggtact
    26041 catgcattat tccaggaaca ggtgcagttt gatcgattag gcctggtcat cgttgacgag
    26101 cagcatcgtt ttggcgtgca tcagcgcctg gcgctgagaa ggaaaggcac gggcacgatc
    26161 gggacgcccc accaattgat gatgagcgcc acgcccattc ctcgcacgct ttcgatgagc
    26221 tatttcgcgg acctggatgt atcagtcatc gatgaactgc cgccaggccg cgccgggatt
    26281 gctacccgat tgtttgctga tactcggcgc gacgaggtga tagcccgcat tcgcgacgcg
    26341 tgccaaagcg gcaggcaggc atactgggtg tgtccgctca tcgaggaatc cgaagccctc
    26401 cagttgaaaa cagcgctgga aacgcgcgaa acgctgagcg ggattttccc ggacttgaga
    26461 atcggtctgg tgcacgggaa gctgccgccg caggaaaaat cggcagtgat ggaagcattc
    26521 gcacaaggcg acatttcact gcttgtggcc acgacagtga ttgaagttgg cgtggatgtt
    26581 cccaatgctt ccctgatgat catcgagcat gccgaacgaa tggggctggc gcagcttcat
    26641 cagctgcgag gccgtgtagg acgtggcgaa gcgggtggac aatgcatatt actgtaccaa
    26701 aaacctttat cggcaaatgc ccgcgagcgt ctcaaaatca ttttcgaaca taccgatgga
    26761 ttcgagattg cgcgccacga cctgcgcctg cgcggtcccg gcgaatttct cggcgcccgc
    26821 cagagcggag tacccatgct gaagttcgcc gacctggagc aggacattga tttgcttgat
    26881 gcggcacgca atgttgcaga tgatctactg tgcaatcacc cgttcgaagc gcaattccac
    26941 ctcgaacgct ggcttggacg aaaaagcgaa taccttcagg tctgaatcat taatccgttc
    27001 ggtgcccgcc gccaggcttc ccccatgatt attcaggttg ataacctgac actggagcat
    27061 cccggaaaaa cgttatgccg cggcttgagc gtgacaatca atgcgggcga atgctgggcg
    27121 atactcggaa agaacggatc tggcaagaca acgctgttga atgcctttgc aggcttgggc
    27181 gagccaaaag atcaggcgga tgcaaggata ctggcagacg ggaaaccgcc tcatgtatgg
    27241 ccgcggcgcg agttttcccg caatctcgga attctgctgc aggaggagcc aggagaattc
    27301 tggggcaatg tcgaggagta tgtgctgctg ggcaggcacc cccatctgag gagtttgttc
    27361 ggctgggacg agaccgatca cgaaatcgcc atgcaagccc tggagcggat ggaactgaca
    27421 gatgtagctc atcgccgtat tggcacgtta tcgggaggag agcgccaacg agcaaggatc
    27481 gccctgctgc tcgcccagtc gccgtcgtgc tatctgctcg acgaaccgct tcagcacctg
    27541 gatctgcgcc atcagttagc cacccttgcg ctgtttcatg aactaaccag gaatggaaga
    27601 actattgtca tggtactcca cgacgcgagt tgggcaaacc ggttctgcag ccacgttctc
    27661 atgctgttcg acaatggctc cgcgacagcc ggcccggtca aggaagtgct caatcgcacc
    27721 aatctcgaaa cgctttatca ttgtcccatg catgaaatcg ggataggagg cgtaagccat
    27781 ttcataccgg cagacgtcgc gccccgtgta taattccggc ttgatgtccc ccttgacgga
    27841 accctggagc cattcctgcg ctttttcggg ttcccaattg catgatcggt aaacttcttc
    27901 gccgcgtctt cacccgcggc aaacctccct cctcccccgt ccccggctcc gcttccggcc
    27961 ccaatgttat tccaagggag gaacatggca tctcgcggga tcacatcaac aaatgcgcac
    28021 taaatgtaac ctcaagcctg caggaggccg gattcgctgc ttttatcgtt ggcggggcgg
    28081 cacgtgacct gatattggga ctggaaccga aggacttcga cgtcgcgaca aatgcgacgc
    28141 ctgaggaagt gcgccccatt ttcagacgct cgcgtatcat tggccgccgt ttccgcctgg
    28201 ttcacgtcat gtgcggacaa gagacagtcg aagtatcgac gtttcggggc gggtcatcag
    28261 gagaagacga gcttgcttca gaagatcata ctgatgacca ggggcggctg ctgcatgaca
    28321 atatattcgg cacgcaggaa gaggatgcga agcgtcgcga tttcactgtc aacgcgctgt
    28381 ttttcgatcc cgttcatgaa gagatctggg attatctgaa cgggtatgac gacatcaagg
    28441 ctcggcgctt gcgcattatc ggcgacccgg agcgccggta tcgcgaggat cccatcagga
    28501 tgctccgggc agtccggctc gccgccaagc tcgatatgca gatcgacgcg gataccgcgg
    28561 ctcccattcg cgatcttgct ccgctgttgc gtaatgtccc cccatcgcgg ctgtttgacg
    28621 agatgctcaa gctgctgctc tccggccacg cgctcacgtg cgtggccgag ctacgcaaac
    28681 gcggattgca ccacggcctg ctccccatgc tggatgtgat actggagcag ccactgggtg
    28741 agcgctttat tacgcttgcg ctaacgaata ccgacgagcg cgtccggcag gaaaagccag
    28801 tttctcccgg atttcttttc gcagcgcttc tgtggcatga agtcctcggc gcgtggaaca
    28861 atcgccaggc ggcgggggaa agaccaattc ctgccctgta cgaggcgatg gatgacgtgc
    28921 tggaagtgca aaacaagaat cttgcaattc cccgccgcta cgatgctgtc atgaaagaga
    28981 tctgggcggc ccagccgcgt ttcgctgcac ggtccgggcg caggccattc cggctgctgg
    29041 agcatcctcg ttttcgggct gcatacgatt tcatgctgct ccgttgcgag agcggagaag
    29101 tggatagcga actgggcaaa tggtggcaga tattccagca tgcgaactcc ggcgaacgcg
    29161 aagcaatgct gatcagggat gaacttctaa acaagcgtaa aagaagcagg ggacgacgca
    29221 aaccagcatt ggcggatacg gttgataacg gaaagctgac ggattccgac gcctcgggtc
    29281 cgaaataggc atggcgcgca ttgtgtcctc gcacgccgtc tgcccggcct tcatagcact
    29341 cggcagcaat ctggccaatc ctgaaagcca ggtactgact gcattcgatg aactggcgag
    29401 gcttcgctcg agccgcctgc tcacgcactc ttcactctat cgaagcgctc ccgtcggacg
    29461 cctcgatcag ccggatttca tcaatgccgc ggcgtacatg gaaactgtcc tgtcccctca
    29521 cgagttgctc gccgccctgc tggatatcga gcgcacacat ggccgagtgc gtgaatgtcg
    29581 tgatgggccg cggacactgg atttagacct cctgatctat aacgatttcg aatgcaatga
    29641 ttcctccctg actctgccgc atccaagaat gcatcaaagg gcctttgtga tccagccact
    29701 tatggaaatc gcgccggatg tccgtattcc cgggcaacct gctctggctg aattgctggc
    29761 tgcatgcgga gggcagcgtc tcgaacgtct gaagaacaac cgggaatgaa gctggaagaa
    29821 taccgacata tcgtgattga aggaccgatc ggggtaggta agaccagtct tgcaaggcgc
    29881 atggcaaacc acctgcgcgg ggaactcctg ctcgaaaaac cggatgacaa cccgttcctc
    29941 gacaggtttt acgaggacat gccccgttac gcactgccaa cccagttgtt ttttctgttt
    30001 cagcgcgccg atcagttaaa gggcctggta caggcggata tgttcgccag gccagttgtc
    30061 agcgatttca tgctcgagaa ggacccgctt tttgcccggc ttacgctaac cgacgccgaa
    30121 tacgacctct atcataaaat atttcgtcac cttcagccca aggcgccgcc gcctggactg
    30181 gtggtttatt tgcaggcgcc cccaggtgta ttgatcgagc gcgtgaagcg gcgcggcaac
    30241 atgttcgaaa gaagtatttc gacggaatac ctgcaacgcc tgtcggacag ctatatgcgt
    30301 ttttttcatc agtacgaagc agctcctctc atgatcatca atagcgaaaa cctcaacttt
    30361 gccgacagtg aagctgattt tgatttattg ttgcaacaaa tagagaagat gcgcgggcat
    30421 cgggaatatt tcaaccgcgg aatttagcga gccgcctgaa caaagggaaa ttactcatgc
    30481 gcaccacaca cgacgtgttc cagaaaatgc gggatgatgg cgagaagatc gccatcctca
    30541 cttgttatga ctcaagtttt gccacattgc tggaaaacgc gggggtagat atcctgctgg
    30601 tcggagattc actcgggaat gtattgcagg gcgaggagag tacccttccc gttaccatgg
    30661 atgacatggt ctaccatacg cgctgcgttg cgcgcggatc gagcaaggca ttcatcatgg
    30721 ctgacatgcc tttcggttcc tcccaggtaa gcccggagga tacttttgaa aatgctgcgg
    30781 aactcatggc cgtgggggcc gggatggtca agatcgaagg gggcagcgtg atggctgaga
    30841 cagtggaatt tcttacccgc cggggcattc ctgtctgcgc acatatcggt ttgacgccgc
    30901 agtctgtgca tcaactgggt ggctataagc ttcaaggacg aacggacgac caagcgaagc
    30961 agttgctgga tgacgccgcc gccctggaaa gtgcaggtgc ggccatgctg ctcatggaag
    31021 tcgttccagc cctgctggcg aaacaggtga cggaaaaact gtctatccct accatcggca
    31081 ttggtgcagg caaggattgc tctgcgcaag tattggtact gtatgacatg ctgggcattt
    31141 attccggcag aaaggcacgc ttcatgaaga acttcctggc cggcggaggg agcattcacg
    31201 aagcagtgac cgattatgtg cgggcagtaa aggcaggcga atttccggga ccggaacacg
    31261 cgttctgatc cccgcgataa gtggaagtca ttaccgatat cgcatccctc cggcagcgcc
    31321 tcaaatacga gccatcgatc gcttttgtgc ctaccatggg caatctccac gccggccatt
    31381 tatctctggt gcgaaccgct cgacaaaagg cccagtgcgc agtagtgagc gtttttgtaa
    31441 atcgcctcca gttcggccca aaggaagatt ttggccggta tcctcgcacc ttggcggaag
    31501 attgcgcgct tctggaaaat gaaggcgtcg atgtcgtatt tgcccccggc gaaactgaac
    31561 tctatcccgt cgcccaggaa ttccaggtcc tgactccgcc gatggctgac actctcgaag
    31621 gtgcgttccg tcccggcttt ttccaaggcg tttcaactgt ggtgctgaag ctattgaata
    31681 tcgtgcagcc cgccgtggtg gtctttggaa agaaggatta ccagcaactc gttctcatgc
    31741 gccagctggt gagagagttc aatcttccgc ttgagattgt tgcgggtgaa accgagcgcg
    31801 cggccgatgg tctggctctc agttcccgca acagctatct tacgctggag gaacgcgccg
    31861 aagcgacacg actgtaccag gtgctgtcac atataaaaca gcaaatggag agcggagctg
    31921 gcaatccgcg tgaactggag gcggacgcga aacaaaatct acaagatcat ggctggcagg
    31981 tggattatgt gaccatatgc gatcgttgta ccttggcgcg ggcaggcgcc cgcgacggga
    32041 atctggtagt cctgggggca gcccgattgg gtaaaacgcg cctgatcgat aatcttgaaa
    32101 tttcaatgga agggtgcggc gacgtacgga agaagcggtg agcgtacgct atcctttcat
    32161 catgtccccc aaccccaacg gcgaatcggg cttccatcta tcggtagcct ggatacccaa
    32221 tgggccaggg tgatcagcaa ctttaagagg agaacaaaat ggccgaatta attgcagtgg
    32281 ggttccagga ccccgaagaa gccgatcgcg ttttgctgcg gcttagcaag ttaaaaaagg
    32341 aatatctgat cgatctcgag gacgcggttg tagtggttcg cgacgaagag ggcaaagtcc
    32401 atctgaagca aagtatcaac ctcgtctcgg cgggagccac ctccgggttt ctcacgggga
    32461 gcatgatggg tgcgctggtc ggcatgttat ttctgaaccc gcttgccggg ttcgtggttg
    32521 gcagcgcctt cggggcaggt gcaggcgcac tgtccggctc gctgtccgat tacggtatca
    32581 atgataattt cataaaatcg gttgctgaaa caattccccg taactcatca gctcttttta
    32641 ttcttgtgcg caaggcacaa ccagagcgcg ttctagctga gctctcgggg gttaaaggca
    32701 aaatcctgca cacttcgcta tcccccgagc aagagaaaaa acttcaggag gcgctcgaag
    32761 gatctaaacc cggtagccag cttgcccctg ggcagtgaca tcacctggaa taccaggaaa
    32821 tggcgctaca ttcaggaatc ggtgaagcag tttaggagtg gaacttaatt ttcccgccaa
    32881 cctgcttcgc tcaatcagaa tcatatctcc ttcaggtatt cgtgcacgaa ttgcatagcc
    32941 attacacctt cacccactgc ggctgtcaca cgctcggttg aacctgaccg aacgtcgccc
    33001 gcagcgaaca cccttggccg gctcgtctca agcaagaacg gctgacggtt caatatccat
    33061 aggggtgaac ttgcaatcga catgccccgt tttgacgaag cccttgttgt cagttgcgat
    33121 gtgcggcagg agccaatccg tatgcgtcgc cgagtagtaa accccagcgc cgtcgaactt
    33181 ctcacgattt tccacatcga gcttgcgata gtcggctccc gtcgcgatca gcaggcaccg
    33241 ggcgaaaacc cgctcgccgt tgtcgagatg caaaaccgga taagcttcgt cgaactccaa
    33301 cgtcgtgact cgtgaaggcg tcgagatctc agcgccgaac ctccgcgctt gaagaacggc
    33361 tcgatcggcc aactcctctc ctgaaacccc ggtcgggaag ccgagatagt tttcgatttt
    33421 cgaggtgcca ccagcttgtc cgcccggagc tgtctgctcc agcacgagcg tgttcaatcc
    33481 ttcgctcgcc ccgtacacag ccgcggccaa ccctgctggc tcccccgccc acgattcatt
    33541 cgacgtgatg atcgacggtc acgggtaagt gggcaattta aagtattccc aagcgcctcg
    33601 gctgtctcaa gccgttccgc aggggcggaa atatgttggg aatgccggga gcggtcaggc
    33661 tctcttcacc tttgaccccc ggttgacggc catgttaatg atccagaggg gattggggcg
    33721 tacggcgacg tccaacgaca caagtaatac tttaaatcaa tatagacaag aaaaacgaaa
    33781 caaaaaagag aatggatggt ccgccttatc gagctcccaa ttctggcctt ttgcagcacc
    33841 ttcctatggc gttaacattc caacagcatt tgagctgcgg aagaccgttt actctgctgg
    33901 cattaaacag ttaagcctga tctgcaacgg caggagttct cccgagcttc ctctgccctc
    33961 cgcaatgaaa cggttgcccc tcgtcgttgt ttaacgacat tcgacatatc ttttgtttca
    34021 aggccttatc cctttatttc tgtttttcgt tgcttgaaag caacagtcct ccaaccctca
    34081 ccgcatagat aactctttgt tttcacctga tttatagatc tggcacgaaa aatgcttaag
    34141 tttaaaagtg tgctaccggt gatacaaaag aggggcgctg ccccggaagc acatcaagta
    34201 gagatgggtg atgtgagctt gtcatggttt tcgtacggct tgacgaaaac cggtcgactt
    34261 gctgagaaat tccacttcaa ggaggtgttt catgttttca ctgcacagtc cgaagcaaaa
    34321 ccgcatcctt gccgccttgc cagcgcaaga ttacgcgcga ctgcttccat ttatggaatt
    34381 cgtgccgatg cctcttgggc aaacaattta tgagccgaat agcgctgtca cgcatcttta
    34441 ttttccaacg accagcattg ttgcgccgat ctacgcggtc gagaacggcg cctcggtccg
    34501 gcttgccatt attggcaacg aaggaatgac cggtacctgc tttcttctgg gtggcggggg
    34561 tacgccttcc ggagtcgtcg tacaaagtga tggatgcggt tatcgaatca aggcaaacac
    34621 ggtaaaaaaa gaattcgaga caggaggaag cctgcaacat gcgattttgc gctatacgca
    34681 agcattgctg acccaaaccg cacagaacgc ggtatgcaac cgccatcaca agatagagca
    34741 gcaactgtgc cggtttctcc tgatgagtct cgatcggttg ccgactgagg aaatgcggat
    34801 gacccacgag cagatagcca tcatgctggg cgtccgcagg gaaagcgtga cccaggcggc
    34861 gctgaaattg cagacgatca acgctatccg gtacagccgg ggacacatta ccgttctgga
    34921 tcgcgaagaa ctcgaaaatg ccgtgtgcga atgctatgag gtggtcaatg acgaatatga
    34981 ccggctgctc tcgaatcgca gcaatatgca ctcgacttcg agacaagtgt ccggcaagga
    35041 gttgtggtcc gtgagttgag gggagccccg gactactcag cgtaaactga caccctcccc
    35101 caacaacggt ttacactggg tagcccggct tgtcgccacg atattgaaag caccgttttc
    35161 agcttgccat caactccctc agcacatacg gcaagatgcc gccattcatg taataatcga
    35221 tctccgtagc cgtatcaatc ctgcacaaca ggtcaacctc gctgcgtgac ccgtcggcgc
    35281 ggtgaatgac gagcgtcaca tcctgctggg gccggatatc tttcaagccc agaagatcga
    35341 attgctcatc cccttttata ccgagtgact gaacatcgtc cgtatccttg aattgcaggg
    35401 ggagtacgcc cattccgacc aggttgctcc gatggatcct ttcgaaactt ttcgcgatta
    35461 ccgccctgac ccctagcagt tgcgtacctt tcgcagccca gtcgcggctc gaacctgtgc
    35521 cgtactcctc tcccgcgaat acgaccgtgg gaacgccgtc cgcaatatat ttcatcgcgg
    35581 catcgtaaat actcatccgc tcgccatccg gctgatgcaa ggtgattccc ccttcgctgc
    35641 ccggaatcat caggttcttg atgcgcacgt tcgcgaaggt gccgcgcatc atcacctcat
    35701 ggttgcccct gcgcgagccg tagctattga agttcgactt ggaaacgccc ttttcaagta
    35761 gatatctgcc cgcaggagaa gtatccttga tcgatccggc ggggctgata tggtccgtag
    35821 tcacggagtc gccaaatatg gccagggcgc gcgcctcccg tatgcccacg ggttgcgaag
    35881 gctgcatcga gaaatcctgg aagaatggcg gctcagcgat ataggtggat tcaggccagc
    35941 tatatacgtc gcccgagacc gagggcacgg tgttccacaa cacatgatcc tcggtgaggt
    36001 tgctataaag gcggcgaaaa atctccccgt ccgcggcata tttgatgaga gcaccgattt
    36061 cttcatcgct cggccagatg tcgcggagcc agaccggctt gccatcttca cctatgccca
    36121 atggttccgc tgtcaaatcc gtgagcatgg tcccggcaat ggcgtacgcc accaccagcg
    36181 gcggagatgc aagaaagttc gcccgtatgc tggggtgaat ccgggcctcg aaattccggt
    36241 ttcccgacag aacggctgca caaaccaggt cattcgccgc cagcgcctcc tcaatgggat
    36301 ccgaaagcgg ccctgaattg ccgatacagg tcgtgcagcc atagcccgca agcgtaaagc
    36361 caagtttttc caggtacggc atcagccctg tcgccgtaag atattccgtt acgacgcgcg
    36421 agcctggcgc aagcgaagtc ttgatgtggt gcttgacgct cagacctttc tccaccgctt
    36481 tttttgccag caatcccgct ccaagaagca cgctggggtt tgacgtattg gtacaagagg
    36541 taatagccgc gatgagcaca tcgccattgc cgaggtcgaa cgccccgttc gagacacatt
    36601 ggggcggctc gtccttttgc atcagggggc gattattgac catttccatg acattccgag
    36661 cgcttcccgg cggcacgtga gcgacgggtt caggagccgc gtcttcacac tccagcgagg
    36721 catcgataag cgccagctcc ggctcttgcg acagattttc cggctccgtt tcgttgcaac
    36781 tcgctacggg ataacgctgc tccagtttct cggcatcctt tccataaccg ccttcattga
    36841 ctggcttgtg caaaagctcg ttaaactttg ctttgatgct tcccatttcg atccggtcct
    36901 gaggtcgccg cggccctgcg agagaagggg taactgttgc cagatcgagt tccagctcac
    36961 gcgtatagtc gatatctccc ttgcgcggca tcccgtacag gttctgcgcc ttgaagtaag
    37021 tctgaaatgc tccgatctcc ttgtcggttc ggcccgtccc cttgaaatag tcgatggtct
    37081 tgtcgtcaac cggaaaaaaa cccattgttg cgccatattc gggagacata ttggcgatcg
    37141 tagcgcggtc aggcacggtg agggacgcag tcccctcgcc gaaaaattcg acaaattttc
    37201 caaccacatg cgctttgcgc aacatttgcg tgacagcgag cacaagatca gtcgctgtca
    37261 ctccacttcg caagtggccc atcaaattca ccccgaccac gtcaggcgta agaaagtaaa
    37321 ccggctgacc cagcatcccg gcctcggcct cgatcccgcc gacgccccag cctaccacgc
    37381 cgatcccgtt gatcatcgtc gtgtgcgagt ccgttccgac aagcgtatcg gggtacacaa
    37441 tgccttcctt cacatgcacg cctcgagcaa gatactccag gttgacttgg tggacgatac
    37501 cgatgcccgg cggcacgacc ttgaacgtat caaaagcctg cattccccat ttaatgaact
    37561 cgtagcgctc gttattgcgc cggaattcca tttccatgtt aatggcgagt gcctgcttgt
    37621 cgccgtaatg gtcgacctgc accgaatgat ccaccaccag atcgacgggc acgaggggct
    37681 cgacaagctt cggatctctg cccattcgct gagcgacgct gcgcattgcc gccaagtcga
    37741 caaggagagg gaccccggtg aagtcctgca acaggatgcg ggataccaca aatggaattt
    37801 catccagtct cggcgcgctc ggcagccatc ccgc
//
LOCUS       DASSHJ010000229         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_709375, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000229 DASSHJ010000000
VERSION     DASSHJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709375"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..763)
                     /locus_tag="VFI43_09010"
     CDS             complement(233..763)
                     /locus_tag="VFI43_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7062302.1"
                     /translation="MKRTMLMLAAALVVPMFSTAAMAEGPDDAQIAAIVVAANQVDID
                     AGKLAESKASNPEVKAFAHRMVADHTDVNKQAVDLVTKLKVTPKENPTSESLKAGGEK
                     NLAHLKTLSGAAFDKAYIDHEVAYHQQVLDAVDKTLIPSAKNEELKALLVKVRPAFVS
                     HLDHAKKVKEMLHKKH"
     gene            complement(1046..>2010)
                     /locus_tag="VFI43_09015"
     CDS             complement(1046..>2010)
                     /locus_tag="VFI43_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7062303.1"
                     /translation="LLAFLVSLFVTFLLVIGTSKSAKFTALLVIIKIIALAAFILLAL
                     PAVNAINFDPMLPNGWGTPLSGVGILGASASIFFAYVGFDAVSTAAEETKNPNRNVPI
                     GLIGSLSVCTAIYLIVAYAAVGAVGAQPGGDLSQSREPLAFVLREVGYPKVGNWVASA
                     AIVALPSVVLMMIYGQTRVLFTMARDGLLPAVFSHVHERYHTPHVVTMVTGVAVALFA
                     GLFPVGMLADISNSGTLFAFFMVSSGVMVLRRRDPLRHRPFKTPVIWLVGPLAMAGCA
                     LLFVSLGWGTIKLFFYWGIIGLIVYFSYARRHSHVGKLPAALRG"
BASE COUNT          501 a          528 c          555 g          426 t
ORIGIN      
        1 acgaagacgg tcaggaggca acgcagaaaa aattttatcc ggtagtccgg aggggggtgg
       61 aacgcaggtg cgatctacat gggatgcggc gaaccgcggg actgaggcta gagaaatcga
      121 gataagtttc ccttcagcca gagggcagta gtgacaacag cttatcacta ctgcactggc
      181 aacccacagg ggatttatcc cggtttctcc agcaggagaa tccgaatatt gctcagtgct
      241 tcttgtgcag catctccttg acctttttcg catgatcgag gtgcgaaaca aacgcgggcc
      301 gcactttgac cagcagcgcc ttcaattcct cgttctttgc gctcggaatc agcgttttat
      361 ccaccgcgtc gaggacttgc tgatggtaag ccacttcgtg gtcgatgtac gccttatcaa
      421 aggcagcgcc gctcagagtt ttaagatgtg cgaggttctt ttcaccgccg gccttcaggc
      481 tctcgctggt cggattctct ttcggcgtga ctttcagctt ggtcacaaga tccacggctt
      541 gtttatttac gtccgtgtga tcggcaacca tacggtgagc gaacgccttg acctcaggat
      601 tggatgcctt ggattcggca agtttgccag catcgatatc aacctggtta gcggcaacga
      661 cgattgccgc aatctgtgca tcatcgggac cttctgccat tgctgcggtc gagaacatgg
      721 ggacaaccag ggcagctgcg agcatgagca tggttctttt cataaatcct ccgcaaaagt
      781 taaaaaaatc gtcgacggtt ggctaacatc gtctcaaggc gcgcctttca attgcccggc
      841 acatatctca gccccaagaa ttcgtacgac ttaagatcgc aattagcccg agggcacttc
      901 aaccttaaga acttgtacac ttgagatcat atacacgagt tcaatgccga cgcaatattt
      961 ttttgaagta tgacgctcag aagtccctgt ttatattatg gtttaaaggg cagatgctat
     1021 gctcaagaga aaagaaggcg gctctttacc cgcggagcgc cgccggaagc ttgcccacat
     1081 gactatgtcg acgtgcgtaa ctgaaataga cgatcagccc tatgattccc cagtaaaaga
     1141 aaagcttgat cgttccccac cccagactga cgaagagcag tgcgcaaccg gccattgcga
     1201 gcggccccac cagccagatg accggagttt tgaagggacg gtggcggagc gggtcgcggc
     1261 gtcgcagcac catgactccc gaggatacca tgaagaaggc gaacagcgtt cccgagttcg
     1321 atatgtcagc aagcattcca accggaaaca ggccggcaaa caaggccaca gcaacgccag
     1381 tcaccatcgt gaccacgtgg ggggtgtggt agcgctcgtg cacgtgggaa aagacggccg
     1441 gcaagaggcc gtcgcgggcc atagtgaaca gcacccgtgt ctgcccgtaa atcatcatca
     1501 ggacgaccga gggaagggca acgattgccg ctgatgctac ccagttgcct actttgggat
     1561 accccacttc gcgcaaaaca aatgccaatg gttctcttga ctgggacagg tcgcctccag
     1621 gctgcgcgcc aacggcgccc accgctgcat aagccacgat caggtaaatc gcggtgcata
     1681 cgctcagaga tccgatgaga ccgatcggaa cgttacgatt gggatttttg gtttcttcgg
     1741 ctgcggtgga gacagcatca aatccgacat aagcgaagaa aattgaagcc gatgctccga
     1801 ggattcccac gccggacaga ggcgtgcccc agccgttggg gagcatggga tcgaaattga
     1861 tggcatttac ggccggcagg gccaggagaa tgaatgcggc gagtgcaatt atcttgataa
     1921 taaccagaag ggccgtaaat ttggcgctct tcgatgtgcc tatcaccaat agaaatgtga
     1981 cgaaaagcga gaccaggaat gccagcaggt
//
LOCUS       DASSHJ010000230         7820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_15907, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000230 DASSHJ010000000
VERSION     DASSHJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15907"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFI43_09020"
     CDS             <1..990
                     /locus_tag="VFI43_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7062304.1"
                     /translation="INGIEELCIGATLADIDHPEALPMPAVDEPTLTMNFQVNTSPFA
                     GKEGKFVTSRQLRERLEKELLTNVAMRLEETGDTDSFLVSGRGELHLTILLENLRREG
                     YELAVSRPRVVTREIDGVKCEPFELLTVDVEEAHQGAVMEALGARRGDLQDMVSDERG
                     RVRLDYRIPARGLIGFQSEFLTMTRGTGMMSHVFDEYAPMRPDIAARRNGVLVSAEQG
                     EAVAYALWKLQERGRMFVNPGDRLYEGMVIGIHSRDNDLVVNPIKGKQLTNIRASGTD
                     EAVTLTPPIQLTLESAIEFIADDELVEITPKTIRIRKRFLLEHERRKASRVAA"
     gene            complement(1090..1587)
                     /locus_tag="VFI43_09025"
     CDS             complement(1090..1587)
                     /locus_tag="VFI43_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HET7062305.1"
                     /translation="MLVIRFKPSRRLALALTLAHFTAIVLLWPLALPVAVKLGASMLL
                     GASLAFYLRCYAWLVSPGSIIGVELSGDMTCMLETRQGKQTPCTLLGSSFVAPYLTVL
                     ELKPLEPLPLWQRVFASPGIVILPDGIDPEQFRQLRVLLRWKWKDAKGKDQKGDQQRD
                     QRGLD"
     gene            1727..2785
                     /locus_tag="VFI43_09030"
     CDS             1727..2785
                     /locus_tag="VFI43_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HET7062306.1"
                     /translation="MNPTWQAYLQDRGATVEDGRVVHYGNPSAELAGVQSATVIADLS
                     HLGLIQFSGEDAQSFLQGQLTCDVRQVDFSSALYGGYCNPKGRMLASFLLCHDNSNYL
                     MQLPLELQPVIQKRLSMYVLRAKVRVADRNEALVRIGVAGNKAEALIAIVSSEIPAGE
                     LGVTHGAGGVSIIRLAQDRFELLLPPGLAQEIWQRLSEDAVAVGAPVWDWLEIRAGIP
                     RITAATQEQFVPQMVNLEMIGGVSFQKGCYPGQEIVARSKYLGKIKRRMYLAHVEPPS
                     DQRVVGAGDELFDVDVGEQSSGMVVNAAASPEGGFDMLAVLQTSSVESGSIRWGHTDG
                     PPLGIMPLPYSVPHLENQ"
     gene            3104..5821
                     /locus_tag="VFI43_09035"
     CDS             3104..5821
                     /locus_tag="VFI43_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HET7062307.1"
                     /translation="MLSFVLAAPASAGALEERDIAFYYGSRPPIEDLRHFDQIVIQPS
                     QILPHEKTALLNLNSLTFAYISIGEVARNSEDMSRIKESWSIGTNPAWNSLVMNMADA
                     DWRDYLLEQHFGRLWREGYRAFFLDTVDSYLIITKEGQPRAEQETGLVALLSEVKRRF
                     PGCKLLLNRGFEVLDRASPYTDGMIAESLFHGFDPVTGKHAPTKEENREWLLNQLARA
                     QNEFKVPVTVLDYVEPGNWAEAEKTAREILKLGYMPWVANGDLTWLGQGRLRLAPRKL
                     LAIINGTPAQQMDHDLFRHAAMPLEYMGLALDYWFIDQLPLPIEPLIGRYAGVVAWLG
                     DEGNLNESKTGRYESMCARLKNEVDGGLPVIFMGYLPAGQSCRNLIDYQGELRPVIRP
                     LDLTKASDQLGRPKTAPVVGSGTPSVHARDRNDAWLTLSDGEKLFNPITVGTRVAYAL
                     HPHIISESASGRREWLLDPFSFFQAALRLPAQPVFDMTTENGRRLGILEVRGDRLFEK
                     DEHGVEVVDRLRAWIEKTGAPVTLGVIEAEVDNEDKREKIKRLASMPQVRLASHTYSH
                     PFYWGVFEGKKDADRQPYRYSVFMDGYAAEVTRETGGTLGFMQSVAPRSPLLLIWSGD
                     GKPVPAALAAAQKAGMPHYGGGGLQWQSGSLSLADLSPMLRPTEWGIQVLTPLTAEPL
                     FARLWYGEALNFGKISEWNKQLDSARRLRVSSISFHADALLHAGGGELLDKLAGEQAN
                     EKVLGIWLDEYVQRARAFQTASIARDFEGNWLLFGDALRTVRLPVSKMIPKVSEDVAG
                     YADRNNDRYIHLARNYAVLKAQPDAAIPDSVVPLRLIEASAPLKSWHLNSNGTATLLF
                     EPRGDLTLQVPGSCMLSSEGATLPALNQGPNFVYTVPFDKASGEFTLEC"
     gene            5811..>7820
                     /locus_tag="VFI43_09040"
     CDS             5811..>7820
                     /locus_tag="VFI43_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7062308.1"
                     /translation="MSAEERRESLLNAPALMFMTAVLLIAFWVLFPRQPAFRDPANLS
                     AKDALSVAYLRVLVQSDPGNAPLRLSFVQLLTEAGMLDEAATAIERLRRAPESALTYE
                     IRLADLRLALQQLYRRPEPQVEESLRAHIAELVPSLLRIARNDRELNQVVASAEQFGE
                     PALLAETFEHLVVQKNEARDKKLRWLVFAGRQRMAANQPRLAARNFLKAFAMEQRARE
                     KLELAKSSLRAYLQAGFDQEVQKEEALKAALQVHENVAADAELLLLGADIAQPLADHA
                     HALAWLEEASQRLLGDQMLAERIVRLQISMGLLADSLSRVAQLRPGLVPGSDRHRLLA
                     QIHDWNGQSDEALEMWLSFARAKADPEAETRAFALARAKPDNGSLVQLLESVMIRRIL
                     TQTEADAYVKAALTIAPPGHAEQQLRQHAERFDNPAATMKALADVLILQGKPGAVLAL
                     REDLPEAENIQQRIDLARLYDEAGNAQKSFDLLHQDLGSPDQRHTEEYWLLLAKVSSG
                     LGQDSYARRAYEKMLALRPGDVEILENLQRLAVRHRDEKESERLARYGWDRLGRIEDL
                     QRLMQFSWKREDWPELDKWLALADEMQSSRPSSVEQADTYWYFRAMRSMASGKRETAE
                     QALRHILQFRGPDPEITEAMIWLLLSGKKVDHAFLDAIVQPFRPQS"
BASE COUNT         1691 a         2091 c         2344 g         1694 t
ORIGIN      
        1 attaatggta tcgaggagct gtgtatcggg gccacacttg cggacatcga ccacccggag
       61 gctttgccca tgccggcggt agacgaacct accttgacaa tgaatttcca ggtgaatact
      121 tcgccttttg ccgggaagga aggcaagttc gtcaccagcc ggcagttgcg cgaacgcctc
      181 gaaaaggaat tactgacgaa cgtcgcgatg aggctggagg aaaccggcga tacggattct
      241 ttcctcgttt ccggccgggg cgagctgcat ctcaccattc tgctcgagaa cctgcggcgt
      301 gaaggttatg agcttgcggt atcacggccg cgagtggtca cgcgggaaat agacggagtg
      361 aagtgcgaac cctttgagct actgactgtg gatgttgaag aggcccacca gggggcggtc
      421 atggaggcgc tcggcgcacg ccgcggtgat ttgcaggaca tggtttcgga tgaacgaggc
      481 cgggtgaggc tggattaccg cattcccgca cgaggtttga tcggctttca atccgaattc
      541 ctgaccatga cccgtggaac aggcatgatg agccatgtat tcgatgagta tgctccaatg
      601 cgaccggata ttgctgcgcg ccgcaatggt gtactggttt ctgccgagca gggtgaagcg
      661 gttgcttatg ctttatggaa actccaggaa cgagggcgca tgttcgtcaa ccccggcgac
      721 cgcttgtacg aaggaatggt catcgggatc catagccggg ataacgacct ggtggtcaac
      781 ccgatcaagg gaaagcaact tacgaatatc cgcgcttccg gaaccgatga ggccgttacc
      841 ctgaccccgc caatacagct cacactggaa tctgccattg aattcatcgc cgacgacgaa
      901 ttggtggaaa tcaccccgaa gaccattcga atccgcaagc gctttctgct cgaacacgag
      961 cggagaaagg cctcgcgcgt agcagcatag cccggcgtac tccgccatgc acggctattc
     1021 cggcattttc cggaaggata acgaaatgca gggataagcc ggggtcctgg agcctgctaa
     1081 tcgactgggc taatccagcc ccctctgatc cctttgctga tcccccttct gatctttacc
     1141 ttttgcatct ttccatttcc atcgcaataa aacccgcagt tgccggaact gctccggatc
     1201 gatgccatct gggagtatga cgatgccggg cgaagcgaac acgcgttgcc acagcggcag
     1261 cggctcaagg ggttttaatt ccagaacggt aagataaggc gcgacaaagc tgctgccgag
     1321 cagtgtgcag ggtgtttgtt tcccctggcg cgtttcgagc atacaggtca tatcgccaga
     1381 tagctcgacg cctattatcg atcctgggga taccagccag gcgtaacatc tcaggtaaaa
     1441 ggcgaggctg gcacccagca gcatgcttgc cccgagtttg accgccacag gtagagcaag
     1501 cggccatagc agcacgatcg cggtaaaatg cgccagggtc agggccagtg cgaggcgtct
     1561 tgatggcttg aaacgaatga cgagcaaggt gaaaaccgga gggcgccgca ggaaaaacag
     1621 gacgggaagg agccataatg cccgcaaatc gcttgagttg atcggccgtt tatcgggata
     1681 aatattggcc gtataatcag tttgatcctt tttcctattg ttttacatga atccgacctg
     1741 gcaagcttat cttcaggacc gtggtgcaac cgtcgaggac gggcgggtcg ttcactacgg
     1801 gaatccctcc gcagagcttg caggcgttca atctgccact gtcattgccg atctctcgca
     1861 cctgggcttg atccagtttt caggcgaaga tgcccagtct tttttgcaag gacaattgac
     1921 gtgcgacgta aggcaggtgg atttctcttc tgccttatac gggggttatt gcaatccaaa
     1981 gggaagaatg ctggcgagct ttctgctatg ccatgacaat agcaactatc tcatgcagtt
     2041 gcctctcgag ttgcagcctg tcatccagaa gcggttgtcg atgtacgtgc tccgggcgaa
     2101 agtcagggtt gctgatcgaa atgaagcgct tgtacgcatc ggggtggcgg gcaataaggc
     2161 cgaagcactg attgcaattg tttcaagcga gattccggcg ggggagcttg gggtaacgca
     2221 tggcgcagga ggggtaagca taattcgcct cgcccaggat cgctttgaac tgctccttcc
     2281 tccagggctc gcacaggaaa tatggcaacg tctgagcgaa gatgcagttg cggtgggggc
     2341 acctgtttgg gattggcttg agataagagc cggtattcca aggattaccg ccgctaccca
     2401 agagcagttc gtgccccaga tggtcaacct ggagatgatt ggaggggtga gttttcagaa
     2461 aggatgctat ccgggccagg aaatcgtcgc gcgcagcaag tatctcggca agatcaagcg
     2521 acgcatgtat cttgctcatg tcgagcctcc atcagaccaa agggtggtcg gggctggaga
     2581 cgagttgttc gacgtggacg tgggggaaca atcgtcaggc atggtggtca acgcagcagc
     2641 ttcacctgaa gggggattcg atatgttagc tgtgttacag acgagcagcg tggagagtgg
     2701 cagcatacgc tggggccata cggatgggcc tcctttgggg attatgccgc taccttattc
     2761 tgttcctcat ttggaaaacc agtgagggtg acagtttgag aaacagcgac gaattgtttt
     2821 gcatgcgctt gctgcatcct gacaaatccc cgaaatgacc aggagcgagg ggattccgtc
     2881 caattattca ccggttttta ttcctccttt caaagatcca gaaggtttag gggattcaaa
     2941 ggtatccgtg gtcttgccac atccgggcga tcctttgagc gcacacctgc acgcgcaagc
     3001 agggagtgtt tgtctgatta ctctcgcttt ctgacgcatg cccaaaacgc ataggatttt
     3061 ccagtcactt atgttttttg cccgatcgta tctcggaggc ctgatgctgt catttgtctt
     3121 ggcggcgccc gcatctgccg gtgcgctgga agagcgcgat atcgctttct actatggaag
     3181 ccgtccgccc attgaagatc tgcgtcattt tgaccagatt gtcatacaac ccagccagat
     3241 cctgccacat gagaaaaccg cgttattgaa cctcaattcg ttgacattcg cgtacatttc
     3301 aattggtgag gtggctcgta acagcgagga tatgagccgc atcaaggaga gctggtcaat
     3361 cggcacgaac ccggcctgga acagtctcgt gatgaatatg gctgatgccg actggcggga
     3421 ttatctgctc gagcaacact ttggtcgcct gtggcgtgaa ggttatcgcg cgtttttcct
     3481 ggacactgta gacagttatc tgatcatcac gaaggaaggg caaccgcgcg ccgagcagga
     3541 aacggggttg gtcgccttgc tctctgaagt caagcgccgc tttcccggtt gcaagttgct
     3601 gctcaacaga ggcttcgagg tgctggatcg agccagccct tatacagacg ggatgatcgc
     3661 cgagtcgctc tttcacggct tcgatccagt cacgggcaag catgcgccga cgaaggaaga
     3721 aaacagggag tggcttctca atcagctcgc gcgcgcgcaa aacgaattca aggttccagt
     3781 taccgtgctc gattacgtcg aaccgggtaa ctgggcggaa gcagaaaaaa cggcgcggga
     3841 aattctcaag cttggataca tgccttgggt ggccaacggc gacctgacgt ggcttggaca
     3901 gggacgtctg cgcctcgctc cgcgcaaatt gctcgccata ataaacggaa cgcctgcgca
     3961 acagatggat catgacctgt tccggcacgc agcaatgcct ctggaataca tgggcctggc
     4021 tctcgactac tggtttatcg accagcttcc gttacccatc gagcctctga taggacgcta
     4081 tgccggggtg gttgcgtggc tcggggacga ggggaatctg aatgaaagca agacgggccg
     4141 ctatgaaagc atgtgtgcgc ggttgaagaa tgaagttgac ggcgggttgc ccgtcatctt
     4201 catgggttat ctgccggccg gccagtcatg ccgtaatctc atcgactacc agggcgagct
     4261 tcgccccgtc atacgcccgc ttgatctcac gaaggcgagc gaccagctcg gccgtccgaa
     4321 aacggcgcct gtagtcggca gtggaacccc ctctgttcat gctcgcgaca ggaatgacgc
     4381 gtggttgacg ttgagcgacg gtgaaaaact gttcaatccc attactgtcg gtacacgagt
     4441 ggcctatgca ttgcatcctc atatcataag tgagagtgca tccgggcgcc gcgaatggct
     4501 gctggatccc ttttcttttt ttcaggccgc gcttcggctg cctgctcagc ccgtatttga
     4561 catgacaacg gaaaatggcc gccggctcgg catcctggaa gttcgcgggg atcgtctgtt
     4621 cgaaaaggac gagcatggcg tggaggtggt tgaccggctt cgcgcatgga tagaaaaaac
     4681 gggagcgccc gttaccttgg gcgttatcga agctgaagtt gacaacgaag acaagcgtga
     4741 aaaaatcaag cggcttgcgt ctatgccgca ggtgcggctg gcaagccata catacagtca
     4801 tcccttctac tggggcgtat tcgagggaaa aaaagacgcc gatcggcagc cttaccggta
     4861 cagcgtattc atggatggct acgcggcgga ggtgacgcgc gaaaccgggg gcacgctcgg
     4921 attcatgcag tccgttgccc cccggtcccc cttgttgctg atctggtcgg gcgacggaaa
     4981 acccgttccc gcagccttgg cggcagcgca aaaagcggga atgccgcatt acggaggagg
     5041 gggcttgcag tggcaaagcg gctccttgtc tctggcggat ctatccccga tgctacgccc
     5101 tacggaatgg ggtatccagg tgctgacccc tttgacggcc gaaccgcttt tcgcaaggct
     5161 ctggtatggg gaagcgctaa attttggcaa gatcagcgag tggaacaagc agctcgattc
     5221 ggcgcggaga ctgcgcgtct cgtccatatc atttcatgct gacgcgctgc tgcatgcggg
     5281 aggcggagag cttctggaca agctggctgg cgaacaggcg aacgaaaagg tgcttggtat
     5341 ctggctcgac gagtacgtcc agcgcgcccg ggcattccag actgcgagta tcgctcgcga
     5401 ttttgagggc aactggctgc tgtttggaga cgccctgagg acggttcggc taccggtttc
     5461 gaaaatgatt cccaaggtct cggaagatgt tgcgggatat gctgatcgca acaatgatcg
     5521 ttatatacat ctggcgcgca attatgcggt cctcaaggcg cagcctgatg cggcaatccc
     5581 tgacagtgtt gtgcccctgc gtttgatcga ggcgagcgca cccttgaaat catggcatct
     5641 caattccaac ggaactgcaa cgcttctgtt cgagccccgc ggtgacctga cactccaggt
     5701 gcccggctcg tgcatgctaa gctcggaagg cgcgacactg cctgccctca accaagggcc
     5761 aaatttcgtc tatacggtgc ctttcgacaa ggcgtcaggg gaattcacgc ttgagtgctg
     5821 aagaacggcg ggagtcgctg ctgaacgcgc cggctctgat gttcatgact gcggtgctgt
     5881 tgatcgcgtt ctgggtattg tttccacgcc agccagcctt cagggatccc gccaatctct
     5941 cggcaaaaga tgccctgtca gtcgcttatc tccgggtcct cgtgcagtcg gatccgggca
     6001 acgctccgtt gcgcctgtcg tttgtccagt tgctaacgga agcaggcatg ctcgatgagg
     6061 cggcaacggc aattgaacgg ctacggcgcg ctcccgagtc cgccctgacc tatgaaattc
     6121 gcctggcgga cctcaggctg gccctgcagc agctttaccg ccgccctgag ccgcaggttg
     6181 aggaatcgct tcgcgcacac atcgccgaac tggttccttc gctgctgcgc attgcgcgta
     6241 atgacaggga gctaaaccag gttgtcgcgt cagcagagca gtttggcgag cctgccctgc
     6301 tcgctgaaac ctttgagcat cttgtcgtcc agaagaatga agcccgcgat aagaaattgc
     6361 ggtggcttgt ttttgccgga aggcagagga tggcagccaa ccagccaagg ttggctgccc
     6421 ggaatttcct gaaggcgttc gccatggaac agcgcgcgcg ggaaaaactc gagcttgcca
     6481 aatcgtccct gcgcgcctat cttcaggcgg gtttcgatca agaggtacag aaggaagagg
     6541 ccctgaaggc ggcgttgcag gtgcatgaaa acgtcgcggc cgatgccgaa ctgctgttgc
     6601 tgggcgcgga tattgcccag ccgctcgcgg atcacgcgca cgccctggct tggctggaag
     6661 aagcaagcca gcgtttattg ggcgaccaga tgcttgccga gcgtattgtg cgcctccaga
     6721 tatcgatggg gctgcttgct gacagtctgt cgagggtagc tcagttacgt cccgggcttg
     6781 tgcccggcag cgaccgtcac cgcttgcttg cccagatcca tgactggaac ggccagtccg
     6841 acgaggcgct tgagatgtgg ctatcttttg cacgcgccaa ggctgatccc gaggcagaga
     6901 cgagggcttt cgcattggcc cgtgcaaaac ccgataatgg atcgcttgtt cagttgctcg
     6961 aatcggtcat gattcgccgc atcttgaccc agacggaggc ggatgcatat gtaaaggctg
     7021 ccctgactat cgccccgcct ggtcacgcag agcagcagtt gcggcagcat gccgagcgtt
     7081 tcgataatcc ggcagcgaca atgaaggcgc ttgccgacgt cctcattctt caaggaaaac
     7141 ccggtgccgt cctcgctctg cgggaggatc tgccggaggc ggaaaatatc cagcaaagga
     7201 tcgatctggc gcgcctgtac gatgaagcgg gaaatgcgca gaaaagtttc gacctgctgc
     7261 accaggattt gggctcgcca gatcagcgcc acaccgagga atactggctg ttactggcca
     7321 aagtatcctc tggactgggc caggacagct acgcgcgcag agcttatgaa aagatgctcg
     7381 ccttgcggcc cggcgatgtc gagatacttg aaaacctcca gcggctggct gtgcgccacc
     7441 gcgatgaaaa agaaagcgag cggctggcgc gttatggctg ggatcgttta gggcgaatcg
     7501 aagatctgca acgcctcatg caattttcat ggaaacggga ggactggccg gaactggaca
     7561 aatggcttgc gcttgcggat gagatgcaat cctccaggcc atcctctgtg gagcaggccg
     7621 acacctactg gtattttcgt gcgatgcgca gcatggcgag cgggaagcgc gagacagctg
     7681 aacaggccct gcgccacatt ctccagtttc gcggtcctga ccctgaaatt accgaagcca
     7741 tgatctggct gttgttgtcg ggcaaaaagg tggatcatgc ttttcttgat gccattgttc
     7801 agcctttccg ccctcagtca
//
LOCUS       DASSHJ010000231         6501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1573600,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000231 DASSHJ010000000
VERSION     DASSHJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573600"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VFI43_09045"
     CDS             complement(<1..162)
                     /locus_tag="VFI43_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit C"
                     /protein_id="HET7062309.1"
                     /translation="MATTMQTSSQARSGGRDYDMSLWYDSRWYKFGLITMLLVAIFWI
                     WYQRTFAYSH"
     gene            462..1115
                     /gene="lolA"
                     /locus_tag="VFI43_09050"
     CDS             462..1115
                     /gene="lolA"
                     /locus_tag="VFI43_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HET7062310.1"
                     /translation="MPAHFLIKHFMNFFQKLSWLLLFYSITSIAEASAIASLKSFIRD
                     TRTVRAAFSQTVLDRNMQIIQKGGGTMQFERPGKFRWIYEKPYSQLIVGDGAKVWFYD
                     QDLDQVTVRKLDAAIGSSPAALLAGSADIETSFDLTEVGSAGGIEWLEAKPKAKESAF
                     EWVRLGFGLDGTLKTMELHDNFGQTTVLTFSQLERNPKIAPEVFKFSPPEGADVISE"
     gene            1108..2421
                     /locus_tag="VFI43_09055"
     CDS             1108..2421
                     /locus_tag="VFI43_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7062311.1"
                     /translation="MSDLFESHQPAAPLAEQLRPRELKDVVGQSHLLGPGKPLRLAFE
                     SGKPHSMILWGPPGTGKTTLARLMATAFDAEFIAVSAVLSGVKDIRDAIERAKAVLQQ
                     SGRHTILFVDEVHRFNKSQQDAFLPFVEQGLVTFIGATTENPSFEVNSALLSRAQVYV
                     LTPLSEEELIVLFERAKRRAPQGLNFTGEARALLIGYADGDARRLLNFLAQIETAARI
                     EGKADISAEYVRNALFRAMRRFDKGGDQFYDQISAMHKSLRGSSPDATLYWLGRMLDG
                     GADPLYIARRLIRAAVEDVGLANPQALRMALDAAETYQRLGSPEGELALAEAALYLAC
                     APKSNAAYVAYNRMRSFISSDQTRPVPEHLRNAPTRLMKELGFGRNYRYAHDEPEAYV
                     GGENYFPQDMPEVKFYEPTSRGGEREISERLARLHELDLGAGKKA"
     gene            2502..3836
                     /gene="serS"
                     /locus_tag="VFI43_09060"
     CDS             2502..3836
                     /gene="serS"
                     /locus_tag="VFI43_09060"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7062312.1"
                     /translation="MIDIQLLRAGLDDVARLLATRGYVFPVVEFSELEAQRKHLQTRT
                     QELQARRNAASKQIGNAKVRGENASSLFEEVANLGHEVKQIEAELESVQAALNHILLQ
                     TPNLPHPTVPIGIGEADNQEVRHWGTPRVFDFPIRDHVAIGETLGLLDFQTAAKLSGS
                     RFSLMTGELARLHRALAQFMLDVHTREHGYQEAYVPYLVNARSLLGTGQLPKFKEDLF
                     EVPRKNSEPVLAVSAGPKHDLEDNNLYLIPTGEVPLTNIVAEKIVPLESLPMRLTAHT
                     PCFRSEAGSYGRDTRGMIRQHQFEKVELVQIAHPQESYEALEELVGHAEKILQKLELP
                     YRVMLLCTGDMGFAAAKTYDIEVWLPAQQAYREISSCSNCESFQARRMLARFRNEKNK
                     PELLHTLNGSGLAVGRTLVAVLENYQNADGTVTIPEALRPYMDGLRLLSTAG"
     gene            complement(3983..4573)
                     /locus_tag="VFI43_09065"
     CDS             complement(3983..4573)
                     /locus_tag="VFI43_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7062313.1"
                     /translation="MAQTRNIITAVKAPELQTSGWLNTPQPLTLASLRGKVVVLHTFQ
                     IFCPGCVQVGIPQAQRIHQEFDPERVAVIGLHTVFEHHSVMGPDALETFVYEYRLRFP
                     IGIDKYEGQPQGVPMTMRAYQMQGTPTLVLIDKNGYIRLHKFGHISDLTVGFSIGALL
                     SEEAEEPSQPEESAQTAASDATASGAACDEDGCSVK"
     gene            complement(4722..5048)
                     /locus_tag="VFI43_09070"
     CDS             complement(4722..5048)
                     /locus_tag="VFI43_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7062314.1"
                     /translation="MKKLIVAAASGTLLLVSAHGMAADVEAGKKKAAEVCAACHGPDG
                     NSPAPNFPRLAGQHADYLAKSLNEYKSGVRKDPIMAGMAAPLSKDDIANVSAYFASQS
                     GLKTKY"
     gene            complement(5074..5403)
                     /locus_tag="VFI43_09075"
     CDS             complement(5074..5403)
                     /locus_tag="VFI43_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7062315.1"
                     /translation="MKRPAIVSVVAWGLLLALTGTAQAAGDPAAGKQKNAMCIGCHGI
                     EGYRTSYPSVYHVPRLGGQHAEYIVKALEAYKSGARNHPSMKGIAASLSTKDMEDLAA
                     YYSSGGK"
     gene            complement(5493..>6501)
                     /locus_tag="VFI43_09080"
     CDS             complement(5493..>6501)
                     /locus_tag="VFI43_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7062316.1"
                     /translation="LNVDQGARSGQMVAELEHVSKAYGNHSVIKDFSCRIMRGDRVGL
                     LGPNGSGKSTLLKLILGELQPDDGTVRRGTKLLVAYFDQMREQLNDETSLVDTISQGS
                     DFIDIGGTRRHVISYLEDFLFAPERARSPVRTLSGGERNRLLLARLFTRPANVLVLDE
                     PTNDLDIETLELLEVLLQGFSGTLFLVSHDREFLDNIVTQVIAFEGDGRFKEYAGGYE
                     DWIRSKKSSLESRESQEKQHSGKPAPQPGETSKPRPVKLGYKETRELEELPAKIEILE
                     KEQADISVQLSSSVIYEGNPMNVMVLQERLVALEQELMQHLDRWEELERKQAAGHKHS
                     I"
BASE COUNT         1483 a         1759 c         1764 g         1495 t
ORIGIN      
        1 gtgcgagtac gcaaacgtgc gctggtacca gatccagaag atcgcgacca gcagcatggt
       61 gatcagccca aatttgtacc acctcgagtc ataccacagc gacatgtcat agtcgcggcc
      121 gccagatctg gcctggcttg atgtctgcat agttgttgcc atgttctacc tcctgttacg
      181 agatactact catacgaata accgatttat ggcagggaat tctccgccag cgccttttag
      241 actctatccg gggacgccta gttccagcac ggcccggatt gaactcgagc cataaatccc
      301 cttcatcagt ctagacccgg tttttgcaaa aatcttgcat gtaattataa aagttgacgc
      361 ggaacaacat tcctgtcaga cggaagtggg agcccatgga attaaaaccg tagtaacatt
      421 ccgggtttcg cgatcatctt gcgcttccac cggatgaatg aatgcccgcc cactttttaa
      481 taaagcattt catgaacttc tttcaaaaat tgtcttggct tttgctgttc tattcgatca
      541 ccagtattgc agaagcctcg gcgatcgcca gcctgaagtc gttcatacgc gacacgcgaa
      601 ccgtacgcgc cgctttttcg caaaccgtcc tagatcggaa catgcaaatc atacagaaag
      661 gcgggggtac gatgcaattc gaacgtccag gcaaattccg ctggatatac gaaaaaccct
      721 attcgcaact catcgtcggc gacggggcaa aagtctggtt ttatgatcag gatctcgacc
      781 aggtaacggt acgcaagctc gacgcagcaa tcggcagcag tcctgctgcg ctgcttgccg
      841 gaagcgccga tattgaaacc agcttcgatt tgactgaagt tggttcggca ggaggcatag
      901 aatggcttga ggccaagccc aaggccaagg aaagcgcgtt cgaatgggtt cgtttgggat
      961 ttggcctgga tggaacgctc aagacgatgg aactgcatga caatttcggc cagacgaccg
     1021 tgctgacttt ttcccagctt gaacgaaacc cgaagatagc accggaggtt tttaaattca
     1081 gcccgccgga gggcgcggat gtgatcagtg agtgatcttt ttgaatcgca tcaacctgct
     1141 gcaccgctcg ccgaacaatt gcggccacgg gaactgaaag atgtagtggg acaatcgcac
     1201 ttgctcggtc cggggaagcc gttgcgtctc gcgtttgagt cgggcaagcc gcactccatg
     1261 atactgtggg gcccgccggg tacaggcaag acaacgttgg cgcggcttat ggcgactgca
     1321 tttgacgccg agtttattgc ggtgtcggcg gttttgtcgg gagtaaagga tatccgggac
     1381 gcgattgagc gtgcgaaggc agtgcttcag cagtctggcc gccacaccat actgttcgtg
     1441 gacgaggtgc accggttcaa caaatcgcag caggacgcgt tcctgccctt tgtcgaacaa
     1501 gggcttgtca cttttatcgg ggctacgacc gagaatcctt catttgaagt gaacagcgcc
     1561 ctgttgtccc gagcccaggt ttacgtgctt actccgttgt ccgaagaaga gcttatagtc
     1621 ctgttcgaac gcgcgaaaag aagggccccg caggggctga attttaccgg ggaggcgcga
     1681 gcgctgctga taggctatgc ggatggcgac gcgcggcgct tgctcaactt tctggcacag
     1741 atcgaaaccg cggcgagaat cgaagggaaa gcggacataa gcgcggagta tgtacgtaat
     1801 gcgcttttta gggcgatgcg acgtttcgac aaagggggcg atcagtttta cgaccagatt
     1861 tctgctatgc ataagtcgct tcgcggctcc agccccgatg cgaccctcta ttggctgggt
     1921 cgtatgctcg acggaggagc ggatcccctt tacattgcga gacgcctcat ccgtgctgcc
     1981 gtggaggacg ttgggctggc caacccgcag gcgttgcgta tggcgctgga tgcagcggag
     2041 acgtatcagc ggcttggaag ccccgaggga gaactcgctt tggccgaggc tgccctctat
     2101 ctcgcgtgcg cgcccaaaag caacgcggcc tacgtcgcct ataacaggat gcgatccttc
     2161 atttcgagcg accagacgcg tcccgttcca gagcatttgc gcaacgcacc gacgcggttg
     2221 atgaaagaac tcggcttcgg acgcaactat cgatatgccc acgacgagcc ggaagcttat
     2281 gtgggcggag aaaattattt cccgcaggac atgccggagg tcaagttcta tgagccgact
     2341 tcacgtggag gtgaacgcga aatttccgaa agactcgcgc gcttgcatga gctggacctg
     2401 ggcgcaggga aaaaggccta gctcatcctg tctttttata ctctgataaa ctaccggcgg
     2461 tttgaccgtt ctcaagccaa actgcggtcg tcaacttttt catgatagac attcaattat
     2521 tgcgcgcggg ccttgacgac gtcgccaggc tgctcgctac acgcggatac gtttttccag
     2581 tagtcgagtt cagcgaactc gaagcgcagc gcaagcatct ccagacgcgg acccaggaat
     2641 tgcaggcacg tcgaaacgcc gcgtcgaagc agatcggtaa tgccaaggtg cgaggcgaaa
     2701 acgcctcctc cctattcgaa gaagtcgcaa acctgggtca cgaggtcaag cagatagagg
     2761 ccgagctcga gagcgtccag gcggcattaa atcacatctt gctgcagacg cccaacctgc
     2821 ctcatccaac cgtacccata ggcattggag aagcggacaa ccaggaagtc cggcattggg
     2881 gtacgccccg cgtgtttgat tttccaatac gggatcatgt cgccattgga gaaaccttgg
     2941 gcttgctgga ttttcagacg gccgcaaagc ttagcggttc gcgcttcagc ctcatgacag
     3001 gggagcttgc gcggctccat cgcgctctcg cgcagttcat gctcgatgtc catacacggg
     3061 agcacggcta ccaggaggca tatgtgccct atctggttaa tgcccggagc ctgttgggga
     3121 ctggtcagtt gcccaaattc aaggaagacc tgttcgaagt tcccagaaag aacagtgaac
     3181 cggtactagc tgtttccgct ggccccaaac atgatcttga agataataac ctttacctta
     3241 ttccgacggg cgaagtgccg cttacgaaca ttgtcgcaga gaaaatcgtt ccacttgagt
     3301 cacttcccat gaggctgact gcccataccc cctgcttccg gtcggaggcg ggaagctatg
     3361 gcagggatac gcggggaatg attcgtcaac accagttcga aaaggtggag ttggtccaga
     3421 ttgctcatcc gcaagagtcg tacgaggccc tggaagagct tgtggggcac gccgagaaaa
     3481 tacttcaaaa gctggaacta ccgtaccggg taatgctgct atgcacgggc gatatggggt
     3541 ttgcggcagc aaaaacctat gacatcgagg tttggcttcc agcgcagcaa gcctatcgtg
     3601 aaatttcatc atgcagcaac tgcgagtcat tccaagcgcg gcgaatgctc gcacgttttc
     3661 gcaacgagaa gaacaaacct gagctgttgc acacgctcaa cgggtcgggg ctggcagttg
     3721 gtcgtacgct tgttgccgtt cttgaaaatt accagaacgc cgatggtact gtgacgattc
     3781 ccgaagccct gcgaccctat atggatggac ttcgactgct ttccacagca ggataaaagc
     3841 ttctggcaac gggacatcca gaaagaaaac ttggaggaga accgggcatc agcgatgggt
     3901 tcgcttgtcc cactggttat cggagcggag aagctaccga agcagagatt tacccggcag
     3961 gggagttcta atatctaccg agttacttga cactgcaccc atcttcatca catgcagcgc
     4021 cagaagcagt agcgtcagaa gcagcagttt gcgctgattc ctccggttgg gaaggctcct
     4081 cggcctcctc cgaaagcaag gcgcctatcg agaagccgac agtaaggtcg ctgatgtggc
     4141 cgaacttgtg caagcgtata tacccgttct tgtcgatcag gacaagcgtg ggagtacctt
     4201 gcatctgata cgcccgcatg gtcatcggaa caccttgagg ctggccttcg tacttgtcga
     4261 ttccgatggg gaagcggagg cgatactcgt acacaaacgt ctcaagcgca tccggcccca
     4321 tcaccgaatg gtgctcgaaa acggtgtgca ggccaatgac tgcgacgcgt tccggatcga
     4381 actcctggtg tattctctgc gcttgaggaa tgcccacctg gacgcagccg ggacagaaga
     4441 tctgaaaagt atgcagcaca accacttttc ctcttagcga ggcaagtgtc aaaggttgcg
     4501 gggtattgag ccaccccgag gtttgaagtt caggagcctt tactgctgtg atgatattac
     4561 gggtttgtgc catcttttta aaactggata aaagatcgcc aacacagcgg tttccagcaa
     4621 ttgccgttta ccggcgccag ccgaaatcgt tttaccgaac ctctgaagaa acccgctgat
     4681 gccagtacgg gactcgggct tgcttcagag gtcttacaga atcaatattt ggtcttgagg
     4741 ccggattggc tcgcgaagta ggcagataca ttcgcgatgt cgtccttgct tagaggtgcc
     4801 gccatgcctg ccatgatggg gtctttgcgg actcccgatt tatactcgtt caggcttttt
     4861 gcgagataat ccgcgtgttg cccggccagt ctaggaaagt ttggagcagg gctattacca
     4921 tcgggaccgt ggcaggcggc acaaacctca gccgcttttt tcttgccggc ttcaacatcg
     4981 gctgccattc catgagcgga tactaataac agggtgccgc tcgccgcggc aacaatcaac
     5041 tttttcatga ggcttccctt gcgtaatgag tgattatttt ccaccggagc tgtaataggc
     5101 cgcgagatct tccatgtcct tggtggagag tgaggcagca atgcctttca tgcttgggtg
     5161 attacgcgcg ccgctcttat aggcttcaag cgccttgacg atgtattccg cgtgctgacc
     5221 acccaatcta gggacgtgat aaacagaagg gtacgaagtc cggtatccct ctattccatg
     5281 acaaccaatg cacatggcgt ttttctgctt tcctgcagca gggtcgcccg ctgcctgagc
     5341 cgtacctgtt aatgccagga gcagacccca cgcaactaca ctcactatcg ctggtcgttt
     5401 cattattaag tgccctcttt ttttcaaaaa gtgcgactct aaacgatgtg ctagctcggg
     5461 tcaacgaata aaatagcggc tcacgtggga acttatatcg aatgcttgtg tcctgccgcc
     5521 tgtttccttt ccagctcttc ccatcgatcg agatgttgca taagctcttg ttcgagtgcc
     5581 accagtcgtt cctgtaaaac cattacattc atagggttac cttcgtagat gacggacgag
     5641 ctgagttgga cgcttatatc cgcttgttct ttctccagaa tctcgatctt tgccggaagt
     5701 tcttccagtt cccgtgtttc cttgtagccc agcttcacgg gccgaggctt gcttgtttca
     5761 cccggttgag gcgcgggttt cccgctatgc tgtttctcct gcgattcccg ggattccaga
     5821 gaactttttt ttgaccgtat ccagtcctca tagccccccg cgtattcctt aaacctgcca
     5881 tcgccctcga aggcgatgac ctgggtgaca atgttgtcga gaaattcccg gtcgtggctt
     5941 acgagaaaca acgtacccga aaaaccctgc agcaatacct ccagcaactc cagggtctca
     6001 atgtcaaggt cattggtggg ctcgtccagg acgagcacat ttgcgggacg ggtaaacagg
     6061 cgtgccagaa gcagacgatt gcgctctccc cctgaaagcg ttctcactgg cgatcgcgcc
     6121 cgttcgggcg cgaacagaaa atcctcgagg tagctgatga cgtgccttct tgtgcccccg
     6181 atatcgatga aatcggaacc ctgacttatg gtatccacga gactggtctc gtcattgagt
     6241 tgctcccgca tctgatcgaa ataggcaacc aacagcttgg tgcctcggcg cacagtgcca
     6301 tcatcaggct ggagctctcc cagtatcagc ttgagcaacg tcgattttcc ggagccattg
     6361 ggccccagca agcccacccg gtcgccccgc atgatacggc aggaaaagtc cttgataacg
     6421 gaatgattgc catacgcctt ggagacatgc tccagttcag ccaccatctg tcccgatctt
     6481 gctccctgat cgacattcag a
//
LOCUS       DASSHJ010000232         5567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1455256,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000232 DASSHJ010000000
VERSION     DASSHJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455256"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFI43_09085"
     CDS             complement(<1..803)
                     /locus_tag="VFI43_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HET7062317.1"
                     /translation="MSVIPTEPIGSIPRPAALLKEIAEKGHMNPELDPLYEETVKDTI
                     ERFEATGSPVITDGEQRKYHNFWTYSVDGLSNTTPDGFKIPFSGHERRMPKLASGPFR
                     YRVYADSYLETAVRYTHLPIKQAIISPSALSLMYPASEIPGYSRAEFIDDLLREHETE
                     VRRCLAKGAHKVQIDFTEGRLAIKIDPTGELLNSFIDLNNLALSRFTPGELLRIGVHT
                     CPGADCDSTHSADIDYAELLPSLFQLKARNFYIALAGEPDRVRVLKIIRS"
     gene            complement(1102..3192)
                     /locus_tag="VFI43_09090"
     CDS             complement(1102..3192)
                     /locus_tag="VFI43_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7062318.1"
                     /translation="MIKDDEEALLRSVALQTSRAILHARQRAEQDLQQAKKALEDKTR
                     QLDHSLSILRATMDATAEGILVTDEEGNVLRHNELYLQLWPIDSRLVTSACHSDLMAC
                     CASFLKEPQEFLRKTRQIYAEWPPDSYDLLELTDGRVFERYSKIHYIEERYIGRVWSY
                     RDVTARRQAEEAARETRDRLRFMSEVLPLKIFTAQADGNADYFNRQWVEYAGVTLERL
                     LGWEWTKLISSDDANKSMERWRHSLQTGEAFQVDHRLRHANGDYRWHLTRAQAKRAYN
                     GSISMWVGSSIDIDAMKRADEERKQLLASERIARSEAEQVNRVKDEFLATLSHELRTP
                     LNAILGWSQLILQGSMDEQNVQRGMEIIERNARAQNKLIEDLLEMSRIISGKVSLDIH
                     LLDLASIAHAAVESLTPLAEAKGIRIRNFINHATGKAAVDHNRVQQIICNLLSNAIKF
                     TPGGGYVDIIVQRVGPHAEIAVKDSGIGIKPEFLKYVFERFRQADSSSTRRHGGLGLG
                     LAIVKQLVLLHGGTVRAESEGEGQGSSFTVSLPLTPMGAEEIDAHDRQGRFTTCARFE
                     FPGARILVIDDEEDSLELMREILAKCGAVVMAASSAAEALEIVKNEKPSVIISDIGMP
                     DMDGYDFMREVRNLPDTQGGKARAIAVTGFVRPEDRADAVAAGYQMHFSKPVDPRQLV
                     SAVGELLGPSRSAV"
     gene            complement(3590..>5567)
                     /locus_tag="VFI43_09095"
     CDS             complement(3590..>5567)
                     /locus_tag="VFI43_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7062319.1"
                     /translation="KSVENASDLYRSLSILRATVEASADGILVTDSQGRVLHFNERYL
                     RLWGMGPEAAEAGEHVDLLNLCYGYLADPQALLQRTAEIYATWPPQSYDVLELADGRV
                     FERSSQTHQSDEQSVGRIWNFRDITARTYAENARRESDERLCFMAEVMPQKIFTARPD
                     GTLDYVNQQWRDYAGLPPKQMEGWEWTKLIHPDDADETIRQWQHAVRTGDPFQVECRF
                     RQANGNYRWHLSRAQAKRRSNGDISMWVGSNTDIDTMKRADEERRQLLESERSARSEA
                     ERANQLKDEFLATLSHELRTPLNAILGWSQLILQGTMNKEHVQRGLEIIERNAHAQNN
                     LIADLLDMSSIMSGKVRLEMQLLDPGSIAEAAIESIAPAAEAKEIRLDKVIDPASGLV
                     YGDSNRLQQIIWNLLSNAVKFTPKHGSVEVTVERAESHLEIAVRDTGPGIHPEFLPHV
                     FDRFRQADSSLTRNHGGLGLGLAIAYQLVGLHGGTIRAESEGEGKGASFVITLPVAPI
                     RGQLNLDPAFSQVVAELPEKRVLLSGIKVLVVDDDHYACGLIEEALTQCEAVVFVATC
                     ADEALELLRKQKPHVMISDIGMPEKDGYQLIREVRNLPAGQGGRTPAIALTAFVHSQD
                     RTKAMLAGYQMHLSKPVEARELVAAIANLTGWKQ"
BASE COUNT         1162 a         1572 c         1537 g         1296 t
ORIGIN      
        1 gatcgaataa tcttcagcac ccgcaccctg tcgggttctc ctgccagtgc aatataaaaa
       61 ttgcgggctt tcaactgaaa caggctcggc aaaagctccg cataatcgat gtcggcgctg
      121 tgagtcgaat cacagtccgc gccggggcag gtgtggactc cgattcgtaa aagctcaccg
      181 ggagtgaacc gggagagggc caggttgttc agatcgatga agctgttaag aagttctccg
      241 gtcgggtcga tctttatggc cagccttccc tccgtgaagt cgatctgcac tttgtgtgcg
      301 ccttttgcaa ggcaccgccg aacttcggtc tcatgctcgc ggagcaggtc atcgatgaat
      361 tccgcccggg aatagccggg tatctcggag gcgggataca tgaggctcaa ggcagacggg
      421 gagataattg cctgcttgat ggggaggtgt gtatagcgca ccgccgtttc gagataagag
      481 tccgcgtaaa cgcggtacct gaatggccct gacgccagct tgggcatcct ccgttcgtgg
      541 cccgagaagg gaatcttaaa accatcgggg gtcgtgttgg aaagcccatc cactgaatag
      601 gtccagaaat tatgatactt ccgctgttct ccatcagtga ttaccggcga gcctgtcgcc
      661 tcaaagcgct cgatcgtatc ttttaccgtt tcctcataga gagggtcaag ttcgggattc
      721 atatgtccct tttccgcaat ctccttgaga agcgctgcag gccttgggat gcttccgatc
      781 ggttcggttg gtattacgct catggtttat gtccattaca agttgttgtt tattattgtt
      841 ggcgcaagct tataaaagcc ggttcagaaa atttcagcct gacagatacg gtgtcaatat
      901 aaggggccag gtgaatgcgt gttgcatcag atagcccgcg aagatgaaag aatgctaccg
      961 ctcgggcagc aaggagtgtc gctggacagg aaatccacca gtaaaaagga cgaggaaccg
     1021 cggcaggttg acccgggact gtcgcggccg gagcaggtcc ggaagcaagc tgaaacggac
     1081 acccaagcat ctgcttttgc ttcatacagc gctacgcgac ggcccgagca attcgccgac
     1141 agcagagaca agctgacgag ggtccaccgg tttgctgaaa tgcatttgat agccggcagc
     1201 gaccgcatcc gctcgatctt cgggacgtac aaatcctgtc accgcaatag ctcgtgcttt
     1261 tccgccctgg gtatccggaa gattcctcac ttcccgcatg aaatcgtaac catccatgtc
     1321 aggcattcca atgtcgctta ttatcacgct gggcttctcg tttttcacaa tttcaagtgc
     1381 ctctgcagcg ctgcttgctg ccatgaccac ggccccgcat ttcgcaagta tttccctcat
     1441 gagttcgagg gagtcctcct cgtcatcaat gacaagaatc ctggcgccgg gaaattcaaa
     1501 acgcgcacaa gtcgtgaacc ggccctgccg gtcatgcgcg tctatctctt cggcacccat
     1561 cggggtcagg ggaaggctta cagtaaagga ggagccttgc ccctcgccct cgctctccgc
     1621 ccggacagtg cctccatgga gcaacacgag ctgcttgaca atggcaaggc ccagcccgag
     1681 tccgccatgg cgacgagtgg acgaggagtc cgcctggcgg aaacgttcga aaacgtactt
     1741 gaggaactcg ggcttgatgc ctattcccga gtcttttacc gcgatttcag cgtgtgggcc
     1801 cacccgctgt acaatgatat cgacatatcc gcctccaggc gtgaacttga tggcgttcga
     1861 aagcagattg caaatgattt gctgcacacg gttgtggtcg acggccgctt tacccgtggc
     1921 atgattgata aaattcctga tccggatgcc ctttgcttca gcgaggggcg tgagcgactc
     1981 gacagcagcg tgggcaatac tcgcgagatc gagcagatgg atatcaaggc tcactttgcc
     2041 cgagatgatc ctgctcatct ccagcaggtc ctcgatcagc ttgttttgag cgcgagcgtt
     2101 cctttcgatg atctccatgc ccctctggac attctgctcg tccattgacc cctgcaggat
     2161 gagctgcgac catcccagga ttgcattgag aggcgtcctc aactcgtgag aaagcgttgc
     2221 gagaaattcg tccttgacac gattgacctg ctccgcctcg ctgcgggcga tccgctcact
     2281 tgcgaggagc tgttttcttt cctcatccgc ccgcttcatc gcgtcgatat caatgctgga
     2341 ccctacccac atcgatatcg agccattgta ggcacgcttg gcctgcgcgc gggtcaggtg
     2401 ccaacggtaa tccccattcg catgacgaag ccggtgatct acttggaacg cctctccggt
     2461 ctgtaggctg tggcgccagc gctccatgct tttgttggca tcgtcggagc tgataagctt
     2521 cgtccattcc caacccaata gccgctcgag cgtgactcct gcgtattcca cccactgccg
     2581 attgaaataa tccgcattgc catccgcctg ggcggtaaaa atcttgagag ggagcacttc
     2641 cgacatgaac cgcaagcgat ccctggtttc gcgcgccgct tcttcagcct gcctccgtgc
     2701 cgtgacatcc cggtagctcc atacgcggcc gatataacgt tcctcgatat agtgtatctt
     2761 ggagtaacgt tcaaataccc tgccgtcggt gagttccagc aaatcgtaac tgtcgggcgg
     2821 ccattccgcg tatatttgcc tggttttcct gagaaactcc tgcggttcct tcaaaaacga
     2881 cgcgcaacac gccatcaggt ccgaatggca ggccgaggtg acaagcctgc tgtcaatcgg
     2941 ccagagctgg aggtacagct cattgtgacg caatacattg ccttcctcgt ccgtcaccag
     3001 aataccctcg gccgtagcgt ccattgtggc gcgcagaatg gagagagagt ggtcgagttg
     3061 ccgtgtcttg tcttccaggg ctttttttgc ctgctggaga tcttgctcgg cgcgctggcg
     3121 tgcatgcagg atggcacgag aggtttgcag cgcaacggag cgaagcagtg cttcctcgtc
     3181 atctttgatc atacttcccc ttacatttat cctaggataa ttatatgtcg attttactcg
     3241 accgaaagtt ccggcgactg cgcagacgga ttgctgaaaa gaaacggaaa ccggaggtga
     3301 tcgttccggc gggttctaaa gatgccttgg acttgagacg tttatgcctg ataccgaact
     3361 tgcatcacac gcggtaggct gactccgcag accgccggta agggttgctg tctgagcgct
     3421 gctaacagga gacgcagaag accgcaactg ggccaggcac gttatattaa atctacgggg
     3481 ataaaaaaac atgaatttaa cataggaagg cttattgatt ccactttccg gcgcaagatg
     3541 ccgcgcggca gacgtcggtg atcagggtct gccaaaggat gtcttgagcc tattgtttcc
     3601 accccgtcag gttggcgatg gccgcaacca gctcccgggc ttccacgggc ttggatagat
     3661 gcatctgata accggcaagc atggctttcg tcctgtcttg cgagtggacg aatgcggtca
     3721 gagcgatcgc cggtgttctt cctccctggc ccgcaggcag gttgcgcact tcacggatca
     3781 gctgataccc gtccttttcc ggcatgccga tatcgctgat cattacgtgg ggtttttgtt
     3841 tcctcaaaag ctccagcgct tcgtcggcgc aagtggcaac aaagacaacc gcctcacatt
     3901 gcgtaagcgc ctcctcaata agcccgcagg catagtggtc gtcatcaacg acaagcacct
     3961 tgatgccgga tagcaggaca cgcttttcgg gcagttcggc gacaacctgg gagaaggcag
     4021 ggtccaggtt gagctgaccc cggattggcg caacaggaag cgtgatgaca aatgaggcac
     4081 ccttgccctc gccctcgctt tcagcgcgaa tagtacctcc gtgcagcccg acgagttgat
     4141 aggcaatcgc gagcccgagc cccagccccc cgtggttgcg ggtaagggac gaatccgcct
     4201 gacggaaccg gtcgaaaacg tgaggcagga actcgggatg gatgcctggt ccagtgtccc
     4261 tcactgcgat ttcgagatga gattcggcac gctcgacagt cacctcgacg gagccatgtt
     4321 tcggcgtgaa tttgaccgcg ttggagagca ggttccagat gatctgctgg agccggttgc
     4381 tgtctccata aactagtcct gacgcaggat cgataacttt atcgagacga atttcctttg
     4441 cttccgcggc cggtgcgatg gattcgatgg cggcctccgc gatactgcct ggatccagca
     4501 gttgcatctc aagcctgacc ttcccggaca taatgctgct catatccaga agatccgcaa
     4561 tgagattgtt ctgcgcgtga gcattgcgct cgatgatctc cagtccacgc tgaacatgtt
     4621 ccttgttcat cgttccttgc aggatgagct gggaccagcc aaggatggcg ttaaggggcg
     4681 tcctgagttc atgcgaaagc gtagcgagga attcatcctt caactgattg gcacgctcgg
     4741 cctcagacct ggcgctgcgc tcgctctcga gaagctgccg gcgttcctcg tcggcccgct
     4801 tcatggtgtc gatatccgtg ttcgagccta cccacatcga gatatctcca tttgaacggc
     4861 gcttggcctg ggcgcggctg agatgccagc gatagttgcc gttggcctgc cggaagcggc
     4921 attcgacttg gaacgggtct ccagttctta ccgcatgctg ccactgtctg atcgtttcgt
     4981 ccgcgtcatc ggggtggatg agttttgtcc actcccagcc ttccatctgc ttggggggga
     5041 ggcctgcgta gtctctccac tgctgattga cataatcgag ggtgccatcc gggcgggcag
     5101 tgaatatttt ttgcggcatg acttcagcca tgaagcaaag gcgctcgtcg ctttcccttc
     5161 ttgcattctc cgcatatgtt cgcgcggtga tatcccgaaa attccagata cgcccgacgc
     5221 tctgctcgtc gctctgatgc gtttgggaag aacgttcgaa tacccttcca tcggcaagct
     5281 ccaggacatc gtagctttga ggcggccacg tcgcataaat ctcggcagtt cgctggagaa
     5341 gggcttgggg gtccgcaaga tacccgtagc acaagttcaa taggtccaca tgttctccag
     5401 cttccgccgc ctcaggtccc atgccccata agcgcaagta acgctcattg aagtgaagca
     5461 cgcggccttg cgagtccgta acgagaattc catccgctga tgcttcgacc gtcgccctca
     5521 ggatcgacag tgaacgataa aggtcgctcg cgttttcgac cgacttg
//
LOCUS       DASSHJ010000233         5065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_802650, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000233 DASSHJ010000000
VERSION     DASSHJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802650"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFI43_09100"
     CDS             <1..207
                     /locus_tag="VFI43_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062320.1"
                     /translation="SGACWLTPALHVGLVIVGSRHERISGRTALDYCALNSHPGFGTA
                     ALLGAILGAAYMLSFIHRAFFGQS"
     gene            complement(367..1482)
                     /locus_tag="VFI43_09105"
     CDS             complement(367..1482)
                     /locus_tag="VFI43_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062321.1"
                     /translation="MYSNVTRLPIEKRVEWLFNLADRYGSTFRSPEAWLARERYLAKH
                     PTAIAVLKCMDGRINLPAMTNTPPGILMPFRNLGGVFNLGWPHLGEVLAHHVQGWVRA
                     GRRVLFLVTYHFSKGDPHRGCAGFNYDTEAAYAHTQVIRKQMEHIFGQGDIIYPLICG
                     IETDEDTIIVHGRNDDKLDMSTIEPGDKRRLEPRLAALLPDMPAQMRGDLMPLLLGNM
                     ETIAQVRQQAARRERSLDIERREWMICVGRGFDFLHTPNLALIIGPYSPNLDVPIRKA
                     GSIIQANMRTGRIPGDGFLLLASAPYSEIGMDRTGAELKSIFLGRFAAEVIRREYPQL
                     AEKMYVRTAVMDWDTRNLEQISESKTEDLEGELAASF"
     gene            complement(1545..2855)
                     /locus_tag="VFI43_09110"
     CDS             complement(1545..2855)
                     /locus_tag="VFI43_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7062322.1"
                     /translation="MPGLKRGLRLRVALAFAIFCIVVVGTLGISLYIASDDIEEAHIE
                     QIMQTEMDYLLQRYRENHNFVPQVGSNLEKYIIHDSSEESKLPSYLQGLNYRRHKVFR
                     GPEEIRVAVRHSDGTKFLVAYEVGLHDQRQQELRLLIVLSLLSVVGVALVVGYLLAGV
                     LVKQVTDLAERVRHLAPGDGQGTTMIQPGQDEEVAQLAHALDDYQNRITRMLRREQEF
                     TANVSHELRTPITTILTSCELLATESGLSERAKVRVGMIEGAATRMGEQLQALLFLAR
                     EQSLGIMEPVALAECVYEAVEPVCSEIYRKRLNFEVAIEPKAELTLNRQALHTALVNL
                     LRNAVQYTERGFIRVNFSESRLSISDSGIGIEPFYMPLLYERFFRGSTQGEGLGIGLA
                     IVKRICDHYGWLIDVESTPGKGSTFHITFPGSQAGALTNSSQFL"
     gene            complement(2842..3525)
                     /locus_tag="VFI43_09115"
     CDS             complement(2842..3525)
                     /locus_tag="VFI43_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7062323.1"
                     /translation="MHILIIEDDPAIATNLYDFLEARGHSVDAAADGITGLHLAITQK
                     FDGILLDLSLPGMDGITLCRKLRQEANLDTPVLMLTARDTLDDKLKGFDCGADDYLVK
                     PFALKEVEARLVAMHKRHGGKVTSRTLETGDLTFDPKTLSIQFVGKGVKLPPKCIRLL
                     ALMMGEPGRVFSRRELESEVWGDAQGTSDTLRSHMHELRRALSRAGGYDPIETVHGLG
                     YRLISRARA"
     gene            complement(3739..4809)
                     /gene="hemE"
                     /locus_tag="VFI43_09120"
     CDS             complement(3739..4809)
                     /gene="hemE"
                     /locus_tag="VFI43_09120"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7062324.1"
                     /translation="MRKPLNDTLLRALLRQPTEYTPVWLMRQAGRYLPEYNRTRARAG
                     SFLALCKNPDFATEVTLQPLARFPLDAAILFSDILTIPDAMGLGLYFAEGEGPKFERP
                     LREEWEIRALTVPDPFANLRYVMDAVSQIRKALDNSVPLIGFSGSPFTLACYMVEGCG
                     GTDFRQVKTMLYQRPELLHHILHINAEAVTAYLNAQIESGAQAIMIFDTWGGALSHDA
                     YGEFSLRYIAQILAGLRHEYGGARIPSIVFTKGGGLWLEKIADSGCDAVGIDWTVDIA
                     EARRRIGQKVALQGNLDPAVLFASPEVIASQVEKILAGYGRGNGHVFNLGHGISQFTP
                     PENALALVEAVHGLSRKFREHE"
BASE COUNT         1129 a         1479 c         1339 g         1118 t
ORIGIN      
        1 tcaggcgcct gctggcttac gccagcgttg cacgtcgggc tggtaatcgt cggttccagg
       61 cacgagcgga tttccggcag aactgctctt gattattgcg cgctcaattc acatcctggc
      121 tttggcacag ccgcattgct gggcgcaata ctcggcgcgg cctatatgct gtcttttatc
      181 caccgcgcct ttttcgggca atcgtgaatg ttaagctgac tcagctgcag gatctgcgtg
      241 atcagaaagg gacagcgggg ggggggttaa atcgacaaac agggtaaatc gacaaaatcg
      301 gactgccata cggggcgcat tttcagatgc gcccgtgtgc caaggtccag agaaaggcga
      361 acgttgttag aatgaggccg ccagttctcc ctcgagatct tcggttttgc tctcgcttat
      421 ttgctccagg ttgcgcgtat cccaatccat gacagctgtt cgtacgtaca ttttctccgc
      481 cagttgagga tattcgcgcc tgatcacctc tgcggcaaac ctccccagaa agatggattt
      541 gagctccgcc ccggttcgat ccatgccgat ttcgctgtaa ggtgcagacg ctaacaaaag
      601 aaatccatcg ccaggtatgc ggcccgttcg catgtttgcc tgaatgatgc tgcccgcctt
      661 gcggatcggg acatcaagat tgggactgta gggcccgatg atcagggcaa gattgggagt
      721 atgcagaaag tcgaatcccc ggccgacgca tatcatccat tcccggcgct cgatatcgag
      781 agaacgctca cggcgcgccg cctgttggcg cacctgcgcg atggtttcca tattcccaag
      841 cagcagcggc atcaggtccc cccgcatctg cgctggcatg tccggcagca atgccgccag
      901 gcgtggctcc agccttcgct tatcccctgg ctctattgtc gacatatcga gcttgtcgtc
      961 attcctgcca tgcacgatga tcgtgtcctc gtcagtctcg ataccgcaga tcaggggata
     1021 aatgatatcg ccctggccaa agatatgctc catttgcttc cggatcactt gagtatgcgc
     1081 atacgcggct tcggtatcgt agttaaagcc tgcgcacccg cggtgaggat cgcctttgga
     1141 aaaatggtag gtcaccagaa acagcacacg ccggccggct ctcacccacc cttgcacgtg
     1201 atgagcaagc acctcaccca gatgaggcca cccaagatta aacacgccac cgagattgcg
     1261 gaacggcatc agtatgccgg gtggcgtatt ggtcatggca ggaagattga tccgaccatc
     1321 catgcacttc aatacggcta tggctgtcgg atgcttcgcg aggtaacgct cgcgagccag
     1381 ccatgcttca gggctccgga aggtcgagcc ataacggtcg gccaaattga acagccactc
     1441 tacgcgcttc tctatgggta agcgcgtaac atttgagtac attgtcacgc tcccgcggtt
     1501 ttcaagtagt tcaaccgtct aaaaattcaa ccgtctaaat actatcacag aaattgtgag
     1561 gaattggtca aggccccagc ttgagatccg gggaatgtga tatggaaggt ggaccctttg
     1621 ccaggggtgc tctcaacgtc aatcagccag ccgtagtgat cgcatattcg cttcacaatc
     1681 gcaaggccga ttcccaagcc ttcgccttgc gtcgatcccc ggaaaaaccg ctcgtaaagg
     1741 agcggcatat agaagggctc gattcctatg ccagaatcgg aaatcgagag gcggctctcg
     1801 ctgaaattaa ccctgatgaa accacgctcc gtgtattgca cggcatttcg gagcagattg
     1861 acgagcgccg tatgcagcgc ctggcgattg agcgtcagct cggctttcgg ctctatggca
     1921 acctcaaagt tgaggcgttt acgataaatt tccgagcaaa ccggttctac cgcctcatag
     1981 acgcattccg ccagagccac gggttccatg attcccaggg actgctcccg agccagaaat
     2041 agcagtgcct gtaactgttc gcccattcgg gtcgcggccc cttctatcat tcctacccgc
     2101 acctttgctc gttccgaaag accggattcg gtagccagaa gctcgcaact cgttagaatc
     2161 gtggtaatcg gcgtacgcaa ctcgtggctc acgttggctg taaactcctg ttcacggcgg
     2221 agcatgcgag tgatacggtt ctgatagtcg tcgagagcgt gcgcgagctg cgccacctcc
     2281 tcatcctgac cgggctgtat catcgtcgtg ccctgcccat cgcccggtgc cagatgcctg
     2341 acccgctccg ccaggtcggt cacctgcttg acaagcactc cggcgagcag ataccccaca
     2401 acaagcgcaa cgccgacgac ggaaagcaac gacagcacga tgagaagccg gagttcctgt
     2461 tgccgttggt catggagtcc aacttcataa gcaacgagga atttcgtgcc gtccgaatgg
     2521 cggacagcga ccctaatctc ctcgggccct cgaaacacct tgtgacgccg gtagttgagc
     2581 ccctgcaggt aagacggcag ttttgactcc tccgacgagt cgtgaatgat gtatttctcg
     2641 agatttgaac cgacctgcgg tacaaaatta tgattctcgc gataacgctg gagcagatag
     2701 tccatctcgg tttgcatgat ctgctcgata tgcgcctcct caatatcatc ggaagcgata
     2761 tataagctga tgccgagcgt gccgacaacg acaatgcaga agattgcgaa cgccagcgca
     2821 acgcggaggc gaagaccgcg cttaagcccg ggcacgagaa atcagacgat agcccagccc
     2881 gtgcacggtc tcgatggggt catagcctcc tgcccggctc agggcccggc gcaattcatg
     2941 catgtggctg cgcaaggtat cactggtgcc ctgcgcgtct ccccatactt ccgattccag
     3001 ctccctgcgg ctgaatacgc gtccaggctc tcccatcatg agagcaagca gacggatgca
     3061 tttcggaggc agcttgaccc ctttgcccac gaactggatg gacaaagtct tgggatcgaa
     3121 tgtcaggtcg cccgtttcca gcgtgcggct cgtgaccttg cctccatgcc gcttgtgcat
     3181 cgcaacaagg cgcgcttcaa cttccttcaa cgcaaagggc ttgaccagat aatcatccgc
     3241 gccgcaatca aaccccttca gcttgtcgtc cagcgtatcg cgcgcggtaa gcatgagtac
     3301 cggcgtgtcc aggtttgctt cctgccgaag cttacggcat agggtgatgc cgtccatgcc
     3361 cggcaggctg agatcgagca gaattccgtc gaatttctga gttattgcga gatgaagtcc
     3421 ggtaatgcca tctgccgctg cgtccacgct gtggccgcga gcttcgagaa aatcgtacag
     3481 attcgtggca atcgccggat cgtcttcaat aataaggatg tgcacaaaat tgtcctactg
     3541 aaattaccgc aaaacggaga ctccgcaacg gtccctcttt tgacccaccc cgggtttcct
     3601 ttgttccagc acaaggctcc cacttcccaa gaggtggcgc ttgttaataa ccgcttttat
     3661 ctgcaaaagc tccagccgga attctaccag ttccccgaca gtccgacgga atccacgttt
     3721 tgccaattca caaaataact actcatgctc ccggaacttg cgactcaggc catgcaccgc
     3781 ttcaaccagg gccagggcat tttccggtgg ggtaaactgt gaaattccgt ggccgagatt
     3841 gaacacatgc ccgtttcccc tgccataacc ggcaaggatt ttctccactt gcgaggcaat
     3901 cacctccggt gaggcaaaca gcacagccgg atcgagattt ccttgcagag caaccttttg
     3961 gccaatgcgg cgtcgcgctt cagcgatatc aacggtccag tcgatgccta ctgcgtcgca
     4021 accgctatcc gcaatctttt ccagccacaa acccccgcct ttcgtaaata caatgcttgg
     4081 gatgcgtgcg cctccgtatt cgtgcctcag tccggcgagt atctgagcga tataacgcaa
     4141 ggaaaactct ccgtaggcat cgtgagacaa cgcgcccccc caggtgtcga aaatcatgat
     4201 tgcctgagcg cccgattcaa tttgtgcgtt gaggtaggcg gtcacggcct ccgcgttgat
     4261 gtggagaata tgatgcagca attcggggcg ctgataaagc attgtcttga cttgcctaaa
     4321 atccgtgccg ccgcagcctt ccaccatgta gcaggcaagc gtgaagggac tcccggaaaa
     4381 cccgatcagc ggaacgctat tatcgagcgc cttgcggatt tgcgacaccg catccatcac
     4441 gtagcgtaga tttgcgaagg ggtccggcac tgtcagggcc cgaatctccc attcttcccg
     4501 caagggccgc tcgaatttcg gcccctctcc ttccgcaaaa tagagcccca accccattgc
     4561 atcagggatg gtaagaatgt ctgaaaaaag tattgccgcg tccaacggaa aacgggccag
     4621 cggctgcaag gtgacctcag tggcaaaatc cggattcttg caaagggcca gaaaacttcc
     4681 agcacgagcg cgggtgcggt tatattctgg gagatagcgc ccggcctggc gcatcagcca
     4741 gacaggggtg tattcagtgg gctgcctgag cagggctcgc agcagcgtat cgttcaatgg
     4801 ttttctcatt tgttcatggc aaatcgaacc cggcaatgat tccggaaagt gtgtcacaag
     4861 cttgcgctga tcctcctgcc cgctgccctc gcaacttcac tcgccccttt gcaagagcgg
     4921 cgatattacc agcgattgcc tcgggggtat ataccatctt tgccagagtg gaaataggcc
     4981 agggggtcag tattcttaaa gcaagtttca gtagtgacgc gcctcgtcga ttgcaaacaa
     5041 tcgcctatat tcactgattg catag
//
LOCUS       DASSHJ010000234         4397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_366807, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000234 DASSHJ010000000
VERSION     DASSHJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366807"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..624)
                     /locus_tag="VFI43_09125"
     CDS             complement(58..624)
                     /locus_tag="VFI43_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET7062325.1"
                     /translation="MQNIDLTNHFLIAMPAMADPFFSKTLTYICEHSEQGALGLVVNR
                     PIDLTLHELFDQLGISGDEENLGQSRVMFGGPIQLDRGFVLHQPVGKWQSTMVVDSTQ
                     GLALTTSLDILQAVGTGQSPNQLMVALGYSGWAPGQIEHEIAQNAWLTVPALPSIIFE
                     LPSEERLSAAVHLLGIDFACLSDEAGHA"
     gene            782..1279
                     /locus_tag="VFI43_09130"
     CDS             782..1279
                     /locus_tag="VFI43_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HET7062326.1"
                     /translation="MLINKMTPYPAAAFGAGIVSGVVSTLAQVALWWLFAENSWLWML
                     NRDARLAAAIVMGQDVLPTSATLDWQVMAVATLIHFTLSIAYSLAFAFMVSRLDARFW
                     LAAGFIYGLAIYAANMYGMTFIFPWFTQARDWITVLAHIVFGTSLADAYKKLSRRRLR
                     GFTRE"
     gene            complement(1346..1792)
                     /locus_tag="VFI43_09135"
     CDS             complement(1346..1792)
                     /locus_tag="VFI43_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062327.1"
                     /translation="MNEYTDQIAEYFNKVPMWPLVLLAVAIAISGIYELIRRKQHAAA
                     VDEYRAAILSTLSGLYPEPTRWPKSIDTYLRARLPVMQDIIENFKPDVPQEDLKGYNK
                     DWENYLHFCRSEVTDEKCEAAETNAAEPDPKKRFHTLVSNLLHYAK"
     gene            complement(1958..2446)
                     /locus_tag="VFI43_09140"
     CDS             complement(1958..2446)
                     /locus_tag="VFI43_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HET7062328.1"
                     /translation="MRQNEKGLTLIETMMILAAVGILATIAIPPAYQAYENSIAREQV
                     ANALTLLDSVKKPISAFYVENKRWPQKAEFDNLVSVQSGKYIENLTSRVLSGGFQVTA
                     TFKNTGGSAGPAKDNAGRTLVLATADGSKWICNDDTNPDTGVPGLVPGNVLKEHRPDA
                     CK"
     gene            complement(2476..2901)
                     /locus_tag="VFI43_09145"
     CDS             complement(2476..2901)
                     /locus_tag="VFI43_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7062329.1"
                     /translation="MLKFLVAVDGSPASSAAVGTFVQIIGWYKEHPQIHLLNVQLPER
                     GNVPLFIDKESIDLYHREEGMKELQMARDILCKDGISCQAHITVGDPAEMILRYAQES
                     GCDQIVIGARGLGPVRGFLLGSVASKVMQNSTLPVLLVR"
     gene            complement(2935..3660)
                     /locus_tag="VFI43_09150"
     CDS             complement(2935..3660)
                     /locus_tag="VFI43_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7062330.1"
                     /translation="MDIASAEFWVAVLQIIAIDIVLGGDNAIVIALACRRLPEKQRNL
                     GIFWGVAGAIGLRVVLIFFALTLLTIPFLKITAALLLLWIGVKLLQPEPESTGPAVDA
                     STSLFGAIKTVIIADAMMSLDNVVAIAGAARDSIYLVVFGLLVSVPIIVWGSKLVMKL
                     MDRFPVTIVIGAGLLGWIAGDMFLTDPLSKDWVNAQAPFLHWLAPLCGALSVVILGKW
                     LALRQPERKRAVVDLLDDRNKPL"
     gene            complement(3822..>4397)
                     /locus_tag="VFI43_09155"
     CDS             complement(3822..>4397)
                     /locus_tag="VFI43_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7062331.1"
                     /translation="REYFGDGSMRQLAPISPALHLGAERVLVVGVRKTADTPTERVKV
                     DHYPTLAQIGGHVMGSIFLDSLHTDLERLERINHTVGMIPDDRLKDTSMPLRQIDCMV
                     ISPSIEISKIAERHAHTLPRAISLFYRSIGAMRRDGSALLSYVLFEEPFCSALIELGY
                     EDTVARKTEILQFIGIAAKSGEEEKNKGPLV"
BASE COUNT         1003 a         1191 c         1141 g         1062 t
ORIGIN      
        1 gggcctcaaa ccaaaccatg ctgctcgatg tgccgcaact atccgacctg tctgacttta
       61 cgcatgtcct gcttcgtccg acaggcaagc gaaatcgatt cccagtagat gcactgcggc
      121 gctcagacgc tcctcggatg gcagctcaaa aatgatgctt ggcaatgccg ggactgtaag
      181 ccaggcgttc tgggcgatct catgctcgat ctggccaggc gcccatcccg aatagcccaa
      241 cgccaccatt aactggttcg ggctctgtcc agttccaacg gcttgcaaga tatcaagcga
      301 ggttgtgagc gcgagacctt gggtcgaatc cacgaccata gtggattgcc atttccccac
      361 gggctggtgt aggacaaagc cgcgatcaag ctggataggc ccgccaaaca taacgcgcga
      421 ctggcctaaa ttctcttcat caccagagat tccgagttgg tcgaacaatt cgtgaagcgt
      481 gagatcgata ggacgattga caacgagtcc aagcgcgcct tgttcgctat gttcacagat
      541 atatgtcaac gttttcgaaa aaaaaggatc tgccattgcc ggcatggcaa tcagaaagtg
      601 attggtgaga tcgatattct gcatggggtc tgaaggcggg taacggtcga cttcagtcag
      661 tttttccaaa aatcatattg tttcggcttc ttttttagag gttccccagc attttagaac
      721 gaatccgctt ctcatactat gttttcgggc gaggacagga agccggaatg tcatttccat
      781 catgctcatc aataagatga ccccgtatcc ggctgccgcg ttcggggcgg gcatcgtctc
      841 gggagtcgtt tccactctcg ctcaggtggc tctgtggtgg ctctttgccg agaattcgtg
      901 gttatggatg ttgaatcggg atgcccgtct ggctgctgcg atcgtgatgg ggcaagatgt
      961 gctgccaacg tccgcgaccc tggattggca agtgatggct gttgcaacgc ttattcactt
     1021 taccctctct atcgcatata gcctcgcctt tgctttcatg gtttcccgcc ttgacgcaag
     1081 gttctggctt gccgctggct tcatttatgg actggcgata tatgccgcaa acatgtacgg
     1141 catgaccttc atcttcccct ggtttactca ggctagagac tggatcacgg tccttgcgca
     1201 catcgtgttc ggcacttcgc ttgccgacgc ctataaaaaa ctttcgaggc gccgactcag
     1261 agggttcacg agagaatgag agagaatgat attctctacg ctgtcgatat ctcgttatca
     1321 gtagcagatg cgtccgttca ctcttctatt tggcgtaatg caacaggttg gaaaccagcg
     1381 tgtggaatct ttttttggga tcaggttcag cggcgttcgt ctccgccgct tcgcatttct
     1441 cgtctgtgac ctccgatcgg cagaaatgta aatagttttc ccaatccttg ttataccctt
     1501 tcaagtcttc ctggggcacg tcgggtttga agttctcgat gatatcctgc atgacaggta
     1561 atcttgcccg aaggtatgta tcgatgctct tcggccaccg ggtaggttcg ggatagagcc
     1621 cggaaagcgt cgaaaggatg gccgcgcgat attcatccac tgcggcagca tgctgcttgc
     1681 gccgtatcaa ctcgtaaatt ccggaaatgg cgatggccac cgcgagcaaa acgagaggcc
     1741 acatgggtac cttgttgaag tattcagcta tctgatcggt gtattcgttc atattctttc
     1801 cgtcctttta gtggaaaacc acatgccaac caggtacatg acctggatta caagagcggg
     1861 gcaatgaagg tcgattgagc gggtacaagc acaagaagaa cagcacaagc acaaggaaca
     1921 tacccacagc ttggccccac catgtccagc agcgtcgtta tttgcatgcg tcgggtcgat
     1981 gctctttcag aacgttaccc gggacaagtc ccggaacacc cgtatcaggg tttgtatcgt
     2041 cgttgcatat ccatttcgaa ccatccgcgg tcgcaagcac gagtgtacgt cccgcattgt
     2101 ctttcgccgg ccctgcactt ccaccggtgt ttttaaaagt tgcagtcacc tggaacccgc
     2161 cggaaagaac gcgcgaggtc aagttttcga tatacttgcc gctttgcacg ctgacgagat
     2221 tatcaaactc ggccttttgg ggccatctct tgttttctac gtaaaatgcg gagataggtt
     2281 ttttcacgct atcaagcaga gtgagggcat tggctacttg ttccctggca atggaattct
     2341 cataggcctg atacgcagga ggaattgcga tagttgccag gatcccgacc gctgcgagta
     2401 tcatcatcgt ctcgatcagg gtcaaacctt tctcgttttg cctcattcct caaaacctca
     2461 atataagacg tgatattacc tgacgagtag cacaggtaat gtcgagttct gcatgacttt
     2521 gctcgcaacc gatccaagaa gaaatcctct gactggtccg agtcctcgcg ccccgataac
     2581 aatctgatcg caccccgact cctgtgcgta acgcaaaatc atttccgcgg ggtcgcctac
     2641 ggtaatatga gcctggcatg atatgccgtc cttgcataat atgtcgcgag ccatctgcag
     2701 ctccttcatc ccctcctcgc ggtgataaag atcgatactc tccttgtcaa tgaagagggg
     2761 tacatttcca cgttcgggaa gctggacatt cagcaaatgg atctgaggat gttccttgta
     2821 ccatccgatt atctgaacga aggtaccgac tgctgcgctg gaagcaggcg agccgtccac
     2881 ggccacgagg aatttcaaca tgagttctcc ctatttccag aacactacgt gcggttagag
     2941 aggcttgttc ctgtcgtcca acagatcaac gacggctcgc ttcctttctg gttgccgcag
     3001 agccagccat ttgccgagga tcactacgga cagggcgccg cagagcggcg ctagccaatg
     3061 caagaaagga gcttgagcat tcacccaatc cttgctcagg gggtcggtca ggaacatgtc
     3121 tccagcaatc cagcccagaa ggccagcgcc tatcacgatg gtaacaggga agcgatccat
     3181 cagcttcatc accagtttgc tcccccagac gatgatgggc acgctcacca agaggccgaa
     3241 aacgacaagg tagatactgt cccgcgccgc gccggcaatt gcaaccacgt tgtccagact
     3301 catcatcgca tccgcgataa tcacggtttt tatagcgccg aagagggatg tactggcgtc
     3361 taccgcggga cccgtgcttt ccggctcggg ttgaagcagt ttgacgccga tccacaacag
     3421 cagcagggcg gccgtgattt tcaggaaagg gatggtcagc agcgtcaggg caaaaaagat
     3481 cagcacgaca cgcagtccga tcgcaccggc cacaccccag aaaataccga gatttctctg
     3541 tttctcaggc agtcgccggc aggctagcgc gataacaatc gcgttgtccc cacccagcac
     3601 aatatcgatg gcgataatct ggagcaccgc aacccagaat tccgcactcg ctatatccat
     3661 gcatcatggg ttctctcaat gaacccttgc tttggttgct cctggaaatg gagtgcggga
     3721 gaggcgacca agtgaatata ggctgcggag aagttcggtt ccgaaaacag gatttcgtgc
     3781 aaggcgatct cagggacggt gaacaggaag caaactccct attataccag tggccccttg
     3841 ttcttttcct cttctccact ttttgccgca attcctatga actggaggat ctcggttttt
     3901 cgcgcaacgg tgtcctcata gcccagttca atgagtgcac tgcaaaaagg ctcctcgaag
     3961 aggacgtagc tcaaaagtgc cgagccgtct cgtctcattg ctccgatgct gcgataaaaa
     4021 aggcttatag cgcgcggaag ggtatgagca tgtcgctcgg cgatcttgct gatctcaatg
     4081 ctcggagaga tgaccatgca gtcgatctgg cgcaaaggca tcgaagtatc tttcagcctg
     4141 tcatccggaa tcatcccgac ggtgtggttg atgcgttcca gccgctccag atctgtgtgc
     4201 aggctgtcga gaaagatgct gcccatgacg tgcccgccga tctgggcgag cgttggataa
     4261 tgatcaacct ttacccgctc ggtcggcgtg tccgctgttt tacgcacgcc aacaaccagg
     4321 acgcgttccg ctcccaaatg gagcgccggg ctgatgggtg caagctgtcg catggaacca
     4381 tctccaaaat attcgcg
//
LOCUS       DASSHJ010000235         9705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1276153,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000235 DASSHJ010000000
VERSION     DASSHJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276153"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFI43_09160"
     CDS             <1..751
                     /locus_tag="VFI43_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7062332.1"
                     /translation="HEVVGLDPLSDDFRVKPMQPAHFEDWKGIALENNAEIAAQRHAV
                     EAAREEINKQRAGHFPRLDMVAAITRNKSDSPILVGIDALTQSVGVQVNVPIYSGGNV
                     SAVTAQAASNYEKAKADLDTKISQTLVELRKQYSLALTSASRIAALVKSVESASFLVD
                     ATQKSIRGGVRTNLDFLNAQHQLFESKRDLAKARYNYLLGYLRLRKAAGVVGAGDLHD
                     IAGYFVSGNNSSALSTGHAVPNGKTIAKTIE"
     gene            1131..1505
                     /locus_tag="VFI43_09165"
     CDS             1131..1505
                     /locus_tag="VFI43_09165"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HET7062333.1"
                     /translation="MDEEKQRQIASKGGHAAHQKGTAHEFTSEEAREAGRKGGHAAHE
                     KGTAHEFTSEEAREAGRKGGHAAHEKGTAHEFTSEEAREAGRKGGESSHGGSGRAASS
                     SSSRGGSSQEHAKAGSPSHKND"
     gene            1607..1825
                     /locus_tag="VFI43_09170"
     CDS             1607..1825
                     /locus_tag="VFI43_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062334.1"
                     /translation="MSNQIPYPENDPRHHTLKIRKMLGETMKHMREDVTKVSDPKAQA
                     LFEVSAEVLNGLITAYDHFEHRSEEAWR"
     gene            complement(2040..2954)
                     /locus_tag="VFI43_09175"
     CDS             complement(2040..2954)
                     /locus_tag="VFI43_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7062335.1"
                     /translation="MSITRIGISGWRYKPWRGVFYPPGLPQDRELEYASRALPTIEIN
                     GTFYSLQTPQSFKRWHEETPPGFVFSVKAPRYITHILRLRDTDAPLANFFASGIFYLK
                     EKLGPIRGQFPPSFRYDAERMKRFFDLLPFDTGEAAALARHRQRRMTGRARLAIDAVR
                     PIRHAVEIRNESFLDESYIDLLRQHNIAWVIADTAGKWPYREDITADFLYLRLHGDKE
                     LYASGYTDEALTRWATRIRVWREGGQPHDAHLVSPSSTPAHRCRDVYCYFDNDIKVRA
                     PFDAQHLAAKLHETMSSPQSPTPLAEGE"
     gene            complement(2957..3301)
                     /locus_tag="VFI43_09180"
     CDS             complement(2957..3301)
                     /locus_tag="VFI43_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7062336.1"
                     /translation="MKLKNLEFENGFRVVLGNSASQAAEMVLSPGDAEGGAENAHTGA
                     DQWLYVVSGTGTATVNGKRYPLAEGSLILIERGDKHEIRNTGEETLRTLNFYVPPAYG
                     SAGEELPRGQGG"
     gene            complement(3480..4109)
                     /locus_tag="VFI43_09185"
     CDS             complement(3480..4109)
                     /locus_tag="VFI43_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380630.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET7062337.1"
                     /translation="MKTHPGYAPNPADHVGNSLEALRRAAADCHACPLWENATQTVFG
                     EGPSRAPIILIGEQPGDREDLVGRPFVGPAGLILSQALEAAEIPREQVYVTNAVKHFK
                     FEYRGKRRLHKKPVDAEIAACHDWLKREIELVAPRLLVAMGATALHSVLGKATPIKHN
                     RGKVLEYAPGLSLLVTVHPSFLLRVPHEDKDEEYRLFVNDLKLAAPFLR"
     gene            complement(4245..5948)
                     /locus_tag="VFI43_09190"
     CDS             complement(4245..5948)
                     /locus_tag="VFI43_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7062338.1"
                     /translation="MTKPLIRVLLVEDDAVDRMACRRALARNKDYEFLLSEADSGSEG
                     LQLAHDQKPDCVLLDYHLPDLNGLEFLAALTDDTGSVSVPVMMLTGTDNAAIAVEAMK
                     RGARDYLIKDNEHQYLELLPAVIQRVLSERQMALEKRLAEEKLFQAEAKYRSLVETIP
                     AIVYIAELNNTNSFLYISSRISMLGFTPEQWLGNTNILAAQIHPEDRERALEERAHSR
                     STGASLRSEYRLLTRSGKVLWFRDEASVVRDESGRLLFLQGILVDITESKRAEAELWQ
                     HRFTLEQLVAKRTDELARVNKELRQDITERKRIEAELIKAKTDAEKANLAKSEFLSSM
                     SHELRSPLNVMLGFAQLMESGNPPPTSTQLTMLREINSAGWYLLELINKILDLAALES
                     GKLIVSHDTVFMTEVMAECRTMIEPQAKERGIQLIFPPEIRVYVRGDRTRLKQILINL
                     LSNAVKYNREGGSVEVNWSARSDRVRVSIRDTGPGLPPEKLTQLFQQFNRLGQESGSV
                     EGTGIGLVVTKELVELMGGKIGVESAVGVGSTFWFELPSAELIEDTKAIDTAEKLAAK
                     S"
     gene            complement(5996..6409)
                     /locus_tag="VFI43_09195"
     CDS             complement(5996..6409)
                     /locus_tag="VFI43_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7062339.1"
                     /translation="MKITNKPILLVEDDQVDTMTVKRALKEIHVTNPLVHVENGEEAV
                     NYLRDSQRDKPCIMLLDLNMPIMNGIEFLQVVKHDDQLKRIPVVVLTTSEEQQDKVSS
                     FNLGVAGYMAKPVDYRQFVEVMRSIDAYWTISEMP"
     gene            complement(6406..8322)
                     /locus_tag="VFI43_09200"
     CDS             complement(6406..8322)
                     /locus_tag="VFI43_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HET7062340.1"
                     /translation="MLKNLTIRSRLIFVLSFLIAFVLGVQALGLFGISSAIDGLKTVY
                     LDRVVPLKQVGQIESLLLQNRVMLTAALLTPTPEVIKKNTGVIDANIDEITRAWVKYT
                     TTYMTPEENRIASDFAEHHRAFIIDGMNPAVAALRASDIEEAHRLLVEKIRPLYKPVA
                     HSLQKLLALQLQETRQEYNQEQERYWTIRNMFIASVVIVLLLGLSFRLIFNRSIFRPL
                     EGVVQTARAVAAGNFAQQFEIHSKDEIGQLMIALKEMRDSLVETIGRVRASDEHTRAL
                     LSNLIDGVASIDEQGVIRTVNPAAQRIFGYTETELIGENSRMLFPAQEQPQDHEPVIF
                     RKLLQMGKLKSLGVTSEVTGMRKDGSMFPMDLAVVEMNDGDRSMFIIIMRDISERKVA
                     EEQKARLMDELESANEELSSFAYVVSHDLKAPLRAIGALANWLSTDYASKFDEEGREH
                     MRLLVNRVHRMGNLIDGILEYSRVGRVREESVRVDVGQLLRDVIDFIAPPSNVTISVE
                     SELPTLISEPTRVQQIFQNLLSNAIKYMDKPRGEIKISCIAEGDRYWRFMVSDNGPGI
                     DPRHFEKIFQLFQTLAPRDRIESTGVGLALVKKIVEMYGGQIWIESKPGIGSTFFFTL
                     PQTPAPALTIGTET"
     gene            8597..9190
                     /locus_tag="VFI43_09205"
     CDS             8597..9190
                     /locus_tag="VFI43_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET7062341.1"
                     /translation="MMKFNMLTAGLLLATIAAPAAAVDSVFKADLNGASESPPNDSKG
                     TGTATITINKEQGTMHVHESFSGLSGGNTASHIHCCTADPGTGTAGVATTLPTFTDFP
                     SGVTMGTYDHTFDMLKDTSYNPAFISGHGGTANSAFAALTAGLDAHTAYANIHTEKFS
                     NGEIRGFLTPAIPEPETYAMFLAGLGVMGALVRRRRN"
BASE COUNT         2225 a         2576 c         2606 g         2298 t
ORIGIN      
        1 tcatgaggtg gttgggcttg atcccttatc cgatgacttt cgtgtgaaac cgatgcagcc
       61 cgcccatttc gaggactgga aaggcatcgc tctcgagaat aatgctgaaa tcgctgccca
      121 gcgtcatgct gtggaagccg cccgtgagga aatcaacaag cagcgcgccg gccatttccc
      181 gcggcttgac atggtcgctg ccattacccg taacaagtcc gacagcccta tcctcgttgg
      241 aatcgatgcg ctaacccaaa gcgtaggtgt gcaagtgaat gtccccatat attccggagg
      301 taacgtcagt gcggtgacgg cccaggcggc gtccaactac gaaaaagcaa aagccgacct
      361 cgatacgaaa atcagccaga cgctcgttga gctgcgcaaa cagtacagtc tggcgctgac
      421 gagtgcttcc cggatcgccg cgctggtcaa atctgttgag tcggccagct tcctcgttga
      481 cgcaacacaa aaaagcataa ggggaggtgt acggacaaac cttgattttc tgaatgccca
      541 gcatcagttg ttcgagtcaa agcgtgatct tgccaaggcc cgctacaact atctgcttgg
      601 atacctgcgc ttgcggaagg cggccggcgt agtcggggct ggcgacctcc atgatattgc
      661 cggatatttt gtctccggca acaactccag cgccttatcg accgggcacg ctgtgccgaa
      721 tggtaaaacc attgctaaaa caattgaata agctcggcta tgtttgcttc cgaacagagc
      781 aatttatccc accttactag catgtagtga ttctctagct ctcaattcta ataaatggag
      841 ataggaatcc cgcagacatg atgtatgtag cacagtcgga gggcagacat tcaatatcca
      901 acattaggag aatcatcatg gcttcagata gagataggag tagcgataac aggagcggcg
      961 gccatgaaag caagcgaagt tccgcttcca cggacgacga aaaaaaacgt gatagtaaca
     1021 aaggcagcca agcgtctcag ggggggcacg gggcgcatga gaaaggagag gaaagcagcc
     1081 ggcgtggtga gggaaagggc agtggaacga gcaggcgagg atttgcttcc atggatgaag
     1141 agaagcagcg ccagatagcg agtaagggag gccatgccgc ccaccaaaag ggcacagccc
     1201 acgagttcac ttctgaggaa gcgcgcgagg ctggccgcaa gggggggcat gctgctcacg
     1261 agaaaggaac ggcgcatgag ttcacgtccg aggaagcgcg cgaggcaggg cgcaaggggg
     1321 ggcatgctgc acatgagaaa ggaacggcgc atgagtttac ttctgaagaa gcgcgcgagg
     1381 ccgggcgtaa aggtggggag tcgtctcatg gaggctctgg cagagctgct tcctcgtctt
     1441 ccagtcgggg cggttcctca caggagcatg ccaaggccgg cagcccgagt cataaaaacg
     1501 attaaccccc aactcagaat aagccttccg tgtggcgaac gcttgcctac ggagggtttg
     1561 tctgcagctt tcgatcgtac caatgtttcc gttttggaga attcagatga gcaatcaaat
     1621 tccatatccc gaaaacgatc cacgtcacca cacccttaag ataaggaaaa tgctgggcga
     1681 gaccatgaag cacatgcgtg aagatgtaac caaggtgtcg gacccaaaag cgcaagcctt
     1741 attcgaggtt tctgcagagg tgctaaacgg cctcattact gcttacgacc acttcgagca
     1801 tcgaagcgag gaagcctggc gttagctcct tctcaacgcg atgttctcga aacggtcggt
     1861 ctacaggggc aggtgttttc gtaacacgaa cgcgtcatct tttgctggtt tccatcacgc
     1921 ctggaattat caagctgccc taagacgtca aaaccgggac gtcaaaaccg ggacgtgaaa
     1981 atctgatcta ttaattctct ccgcccttcc tcacggtggt ttgactattt gcacccctac
     2041 tactctcctt ccgcaagggg ggtaggagac tgcggagagg acatcgtctc gtgcagcttt
     2101 gctgccaaat gctgcgcatc gaagggcgct cttaccttta tgtcattatc gaagtagcaa
     2161 taaacgtccc ggcatcgatg ggcaggagtt gaactgggcg agacaaggtg ggcgtcgtgc
     2221 ggctggcctc cttcgcgcca cacccgtata cgcgtagccc agcgcgtcag cgcttcatcg
     2281 gtatacccgc tcgcgtacag ttccttgtct ccgtgcaggc gcagataaag gaagtcggca
     2341 gtaatatctt cgcgataggg ccattttccc gcagtgtcgg caataaccca ggctatgttg
     2401 tgttgacgca gcaaatcgat ataggattcg tccaaaaagc tttcgttgcg gatttcaacc
     2461 gcgtgcctta ttggacgcac agcgtcgatg gctaacctgg cacgtccggt cattcgcctc
     2521 tgccggtgac gcgcgagggc cgcagcttct ccggtatcaa acggcaaaag atcgaaaaaa
     2581 cgtttcatcc tttccgcatc gtagcggaag ctcggcggga attgcccccg gatgggccca
     2641 agcttttcct tgagataaaa aataccggag gcaaagaagt ttgccagcgg ggcgtcggtg
     2701 tcacgcaaac gcaatatatg agtgatatac cgaggcgctt ttacgctgaa cacgaacccg
     2761 ggaggcgttt cttcatgcca gcgcttgaag ctttggggcg tttggaggga gtagaacgtt
     2821 ccgtttattt caattgtagg aagggctcgg gaggcatatt caagctcccg atcctgggga
     2881 aggccagggg gatagaagac gccgcgccaa ggcttataac gccagccgga gatcccgatc
     2941 cgcgttatgc tcacctttac ccgccttgcc ctcttggcaa ttcctcaccc gcgctgccat
     3001 atgcaggtgg aacataaaaa ttaagggtac gaagtgtctc ctcgccggtg ttgcggatct
     3061 cgtgcttatc gccgcgttcg ataagaatga gggagccttc ggccaaagga taccgttttc
     3121 cattcactgt tgccgttccc gtccccgaaa ccacgtacaa ccattggtcg gcgcctgtgt
     3181 gcgcgttttc agcgcctcct tcagcatctc ccggcgacag aaccatttcg gctgcctgag
     3241 aggcggaatt gccaagaacg acgcgaaacc cgttttcaaa ctcaagattt ttaagtttca
     3301 tcccttattc caccgagcga agcgagagag ggaagtgcac gtgagcaagc agtctgatca
     3361 agacgattga acttggacag ccgaaacggg ccacggtttc tctttctgtg attcgaggtt
     3421 agatcccggt accgactcca tgccaaaatt ctggccggcg cagcgttggt catcgaagat
     3481 caccgcagga acggtgcggc gagtttgaga tcgttgacga agaggcggta ttcctcatcc
     3541 ttgtcttcgt gcggaacgcg taacaggaag gaggggtgga cagttaccag cagtgagagg
     3601 ccgggagcgt attcgagaac ttttccccgg ttatgcttga taggcgttgc cttgcccaac
     3661 actgaatgca aagctgttgc tcccatcgcg acaagcaggc gaggagcaac gagttctatc
     3721 tctcgtttca gccagtcgtg gcaggctgca atctcggcat caacgggttt cttgtgcagg
     3781 cggcgcttgc cgcgatattc aaacttgaaa tgcttgactg cgttggtgac gtagacttgc
     3841 tcgcggggga tttctgcggc ctccaacgcc tgagacagaa tcaggccagc gggcccgaca
     3901 aatggacggc cgacgagatc ttcgcgatcc ccaggttgtt ccccaataag tatgattggg
     3961 gcgcgtgaag gtccttctcc aaaaacggtc tgcgtggcgt tttcccataa gggacacgcg
     4021 tgacagtcgg cggcggctct acgcaaagcc tccagtgaat tacccacgtg atctgcagga
     4081 ttgggcgcgt atcccggatg ggttttcatg gttcgaatcg cgacaaacgt ggagtacggc
     4141 tggttttggg ctggacttgg gctggacttg gatcggattt gttgccttga acgccaggac
     4201 cagctttatg aaaagaaagc cccggtaatc ctctgcctga aaatctagct ttttgctgca
     4261 agcttttcgg cggtgtctat cgctttagta tcttctatga gctctgcgga aggaagttcg
     4321 aaccagaagg tgcttcctac ccctacagca ctctcgacgc caatcttccc gcccatcaac
     4381 tcgaccaatt ccttggtgac aaccagccca atgccggtgc cttcgacgga tccggattcc
     4441 tggccgagcc gattgaattg ttgaaagagc tgtgtcagtt tttcaggtgg aagtcccggt
     4501 cccgtgtccc ggatactcac acgaacacga tctgaccgcg cgctccagtt cacttcgaca
     4561 gagccaccct cgcggttgta tttgaccgcg ttggaaagaa gattgatcaa tatctgcttc
     4621 agtcgcgtac ggtcacctct gacgtaaacg cgtatttccg ggggaaagat gagctggatg
     4681 cctcgttcct tcgcttgagg ttcaatcatg gttcggcact cggccatcac ctcggtcatg
     4741 aagactgtat catgcgatac gatcagcttg ccggattcaa gcgcggccag atcgaggatc
     4801 ttgttaataa gctccaacag gtaccaccct gccgagttga tttcccgcag catcgtcaat
     4861 tgagtggaag tgggcggcgg gttacccgat tccataagtt gtgcgaaacc gagcatgacg
     4921 ttgagcggcg accttaactc atggctcata cttgaaagaa actcggattt cgccagattt
     4981 gctttctctg catccgtctt ggccttgatc agctctgctt cgatacgttt tcgttcggta
     5041 atatcctggc gtaattcctt gttaacccgg gcaagctcat ccgttctctt cgctaccagt
     5101 tgctcgagcg tgaagcggtg ctgccaaagt tctgcctcgg ctcgtttgct ttcagtaata
     5161 tccacaagaa taccctgcag gaacaggagt cggccagatt catcccgaac cacgctcgct
     5221 tcgtcccgga accacaaaac tttgccgctg cgcgtaagca ggcgatactc cgaacgtagc
     5281 gaagcgccag tggatcggct gtgcgcccgt tcctccagcg cgcgctcgcg atcctcagga
     5341 tgaatctggg ccgcgagtat gttggtatta cccagccatt gttctggcgt aaacccaagc
     5401 atgcttatcc tggagctgat gtagagaaaa ctgttggtgt tattcagttc ggcaatgtaa
     5461 acaattgcag ggattgtctc gaccagggaa cgatatttag cttctgcctg gaacagtttc
     5521 tcttctgcga gtcttttctc gagcgccatc tgtcgctcgc tcaacacgcg ctggataact
     5581 gcaggcaaca actcgaggta ttggtgttcg ttgtccttga tcagataatc ccgcgccccc
     5641 cgcttcatgg cttccaccgc aatggccgca ttgtccgtgc ctgttagcat catgacagga
     5701 accgacacac tgccggtgtc atctgtaagc gcagctagaa actccaggcc gttcaggtct
     5761 ggaagatggt aatccagcaa gacgcaatcg ggcttctggt cgtgggcgag ttgtaacccc
     5821 tcgctcccgg aatcggcttc ggaaagcaag aattcgtagt ccttgtttcg ggcgagggcg
     5881 cgccggcacg ccatgcgatc cactgcatca tcctccacaa gtagcacacg aatcagcggt
     5941 ttggtcatat gaaggctcag agctgttttg ccggcatgag atgttttcgt tcgccctacg
     6001 gcatttcgct gattgtccag taggcgtcaa tcgagcgcat aacctcgaca aattgccggt
     6061 aatcgaccgg ttttgccatg taaccagcta cccccaaatt aaaactgctc accttgtcct
     6121 gctgctcctc ggacgtcgtg aggacgacca ccggaatacg ctttaactga tcgtcatgct
     6181 tgaccacctg cagaaattca atcccgttca taatcggcat attcaggtcg agtagcatga
     6241 tgcacggttt atcacgttgc gaatcccgca gatagttgac cgcctcttcg ccattttcta
     6301 cgtggacgag agggttagtt acatgaatct ctttaagggc gcgctttacc gtcatcgtat
     6361 caacttgatc gtcttctacc aggaggatgg gcttgttggt gattttcacg tttccgttcc
     6421 tatagttaaa gcaggtgctg gggtttgggg tagcgtaaag aaaaatgtgc tgcctattcc
     6481 gggtttcgac tcaatccata tttgtccccc atacatttcg acaatttttt tcacaagggc
     6541 cagaccgaca cccgtgcttt ctatgcggtc tcgtggagcc agggtttgaa acagctgaaa
     6601 aattttttca aagtggcgcg ggtcgatgcc aggcccgtta tcgctgacca taaacctcca
     6661 gtagcggtcg ccttccgcaa tacagcttat ttttatttcg ccccgcggct tatccatgta
     6721 tttgatagcg ttggatagca ggttttgaaa tatttgttga acgcgggtgg gctctgatat
     6781 cagggtgggt aattcgcttt caacactaat ggtaacattt gaaggcggag cgatgaaatc
     6841 tataacgtcc cttaaaagct gacctacgtc aacacggacc gattcttccc tgacccggcc
     6901 gacgcgtgaa tactcgagga tgccgtcgat cagattcccc atacgatgca cccgattgac
     6961 gagcaggcgc atgtgctcgc gtccttcttc atcgaatttt gacgcatagt ccgttgaaag
     7021 ccagtttgcc aacgcgccaa tggcacgaag cggcgctttt agatcatgtg agacaacata
     7081 ggcaaaactg ctcagctctt catttgcgct ttccagttca tccatgagtc gcgctttttg
     7141 ttcctccgcc acttttcgct cactgatgtc cctcattata atgatgaaca tactgcgatc
     7201 gccgtcattc atctctacta cagcgagatc cattggaaac atcgacccgt ccttgcgcat
     7261 tcccgtcacc tcgcttgtca cccccaggct tttaagtttt cccatctgaa gaagcttcct
     7321 gaaaataacc ggttcgtgat cctgaggttg ctcttgagcg gggaaaagca tccgtgagtt
     7381 ttccccgatc agttcggtct ccgtgtagcc gaagattctc tgtgcggctg gattgacagt
     7441 cctgattacg ccttgctcat caatcgaggc gactccatcg atcaggttgc ttaacagggc
     7501 gcgggtatgt tcatcgcttg cccgcacccg cccaatggtc tccacgaggc tatcgcgcat
     7561 ttccttgagc gcgatcatga gttgcccaat ctcatccttc gagtgaattt caaactgctg
     7621 ggcgaaattg cccgctgcga ccgcccgtgc ggtttgcacc acgccttcaa gcggtcgaaa
     7681 aatcgagcga ttgaagatca gccggaatga taagcccaac agcaagacaa tcacgaccga
     7741 cgcaataaac atgttgcgga tcgtccagta gcgctcctgc tcctggttgt attcctggcg
     7801 tgtttcctgt agttgcaacg caagcagttt ctggagacta tgtgcgaccg gcttgtagag
     7861 ggggcgaatt ttttccacca gaaggcgatg cgcttcctct atgtcgctcg cacggagcgc
     7921 ggccacggcg gggttcatgc cgtcgatgat aaacgcgcgg tgatgctcgg cgaaatcgga
     7981 cgcaattctg ttttcctccg gcgtcatgta cgtcgtggtg tatttgaccc aggctctcgt
     8041 aatctcgtct atatttgcgt cgattacgcc ggtgtttttt ttaatgacct cgggcgtcgg
     8101 cgtcaggagg gcagctgtca gcatgacgcg gttttgcagc agaagcgatt cgatctgccc
     8161 cacctgtttc agtggcacca ctcgatccag gtaaacggtt ttcaatccat caattgcgct
     8221 gctaataccg aacaggccga gtgcctgaac cccaagcacg aaggcaatca aaaacgaaag
     8281 aacaaagatc aggcgggacc tgatggttaa gtttttgagc atatgccggt aactatgggt
     8341 tatcgggttt tgccattgtt gccggagctt tttctgatgg cgccacttca aaactccttt
     8401 tgtgatcagg gtgaaatatt accgcatgta tctgaaatga aaaaattcct ttttaagaac
     8461 ttaagcaaaa agtggccggg ataaatgaaa tccataataa gcccaacgga ctattgaaaa
     8521 gtcgggctca ccatataaga tttgagtggg ccctctcaaa gtagatgacc catcccataa
     8581 aacgaaggag gtagatatga tgaaattcaa catgcttacg gcaggactgt tgctggccac
     8641 gattgccgct ccggctgccg cggtcgattc ggtttttaag gctgatctca acggtgcgag
     8701 cgaatcgccg cccaatgatt ccaagggaac tggcacagca acgattacca tcaacaagga
     8761 acagggcacg atgcatgttc atgagagctt cagcggccta tcagggggta atacagcctc
     8821 acatattcac tgctgtacgg cggacccggg aacaggaacg gcgggggtgg ccacgacttt
     8881 gcctacgttt actgatttcc cttcgggagt aacgatgggc acttatgatc atacctttga
     8941 tatgcttaag gacaccagct acaatcccgc tttcatttcc ggacatgggg gaacggcgaa
     9001 tagcgccttc gccgcactta cggccggtct ggacgcacat actgcctatg cgaatattca
     9061 taccgagaag ttttcgaatg gcgaaattcg cggtttttta acgcccgcca tacccgaacc
     9121 cgaaacttac gccatgtttc tagcgggact aggcgtgatg ggcgcgctcg ttcgccgtcg
     9181 caggaattga tgccgtagca gctccgacgg attttgcaag tgagcggagc gagctagacg
     9241 gccgccagac gagcttcgga tactgttgcc actgttcatc ttaaggcggc gtgtgctgcc
     9301 ggtggcccgc agttatggca gccgccgccc agtcaaggtg ttaaagacaa aatagctttt
     9361 tgttggtccg ccacgagcgc ggcgcctcat gaaggaagct gccttgcgtc ctgtatggcg
     9421 aaagtttatc cgcacaacgg gcagcaggca tggtttgcta acggcaggca atctgctgga
     9481 gcaaaacttc aaagttacca ggccaatgtt gcctgagtgt cgggtatcac ccaggtgcgc
     9541 aggcacgtgg ctggctctat ctttctgaag taacgggccg gttctcgtgc aagacgcacc
     9601 aggaagcgtg tccccagctg tgttccgtga gcaattgctc gcagggaaaa attctacttc
     9661 tgaactgtct ccttgacgga gaagtttacg cttccagcgc cagcg
//
LOCUS       DASSHJ010000236         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_149480, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000236 DASSHJ010000000
VERSION     DASSHJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149480"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VFI43_09210"
     CDS             complement(<1..1420)
                     /locus_tag="VFI43_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7062342.1"
                     /translation="MKKPISRILVINDEKLILKELIKGLNSAARSLENPLGITFSGVT
                     TAREALQAIQEDGDIQSVVVDDTLYTLKNGEKGARNLQMTALKLVQEINRFRPELDIY
                     ILIAQEKEDEVVDALFSETVDGYFYREERDYRGMYRILNAQLQEKARTPFYDQLKNYV
                     LMAKDAWHTPGHSSGDSVRDSPWASDFYHFVGDHVFRADLSVSVPMLDSLMEPTGVIA
                     EAQKIAAKAFGARRTFFATNGTSTANKVIFQTLLAPGEKLILDRNCHKSVHHGVVLSG
                     AYPIYLDSSVNKKFSIYGPGPKQTLFTAIEEHPDAQAMILTSCTYDGLRYDLPPIIEA
                     AHAKGIKVIIDEAWYAFARFHPAFRPTAMEAGADYATQSTHKVLSAFSQSSMIHVNDP
                     GFNEHLFRENFNMHTSTSPQYTMIASLDVARKQAMMEGYKLLSRTLELAKELRAQINS
                     TGVFRVLDLSDLLPAEVSGDNIL"
     gene            complement(1618..1881)
                     /gene="rpsT"
                     /locus_tag="VFI43_09215"
     CDS             complement(1618..1881)
                     /gene="rpsT"
                     /locus_tag="VFI43_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7062343.1"
                     /translation="MANSAQARKRARQNISHHHRNMSLRSQLRTAIKHVRKGIASADK
                     ATAQAAFQASVGTIDSIADKGIIHKNKAARHKSRLVAAIKAMA"
     gene            2096..3655
                     /gene="murJ"
                     /locus_tag="VFI43_09220"
     CDS             2096..3655
                     /gene="murJ"
                     /locus_tag="VFI43_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7062344.1"
                     /translation="MNLLKTLATVSSMTLISRILGFVRDMLVARVFGASVATDAFFVA
                     FRIPNLLRRLFAEGAFSQAFVPILSEYKNKRTEDQTRELINKVSALLSIALFTITLIG
                     VIAAPIVIYISAPGFSASPQKFGLTVDLLRITFPYILFISLVSLAGGILNTYGKFSIP
                     ALTPALLNVSFIGCTLWLAPLLDPPVLALAWAVFIGGALQLAFQVPFLMKIRLMPCPS
                     LNSDDPGAWRIVRQMGPAVFGVSISQISLLINTIFASFLVTGSVSWLYYADRLMEFPA
                     GLLGVALGTILLPSLSRHYAQNSMEKYSRLLDWGLRLTMLLTVPAALALALLATPLVA
                     TLFFHGQFSPNDVWMTRDAVVAYSVGLLGLILVKVLAPGFYARQNIKTPVKIAILTLV
                     MTQMMNLAFIVPLRHVGLALAIGLGACLNAALLYYKLRSQNIFQPQPGWAIFIAKILT
                     ASSGMGVLLWVVSGSDASWLADSTFDRVLRLVYIVALGGASYFAMLWVLGFRLSNFSK
                     HAAGTDIGDKE"
BASE COUNT          860 a         1103 c          981 g         1011 t
ORIGIN      
        1 gcagaatatt gtctccactc acttccgcgg gcaacaggtc cgaaaggtca agaaccctga
       61 atacacccgt cgaattgatc tgggcgcgca attccttcgc cagctccaac gtccgcgaaa
      121 gcagcttgta cccttccatc atcgcctgct tgcgagctac gtccaggctc gcaatcattg
      181 tgtattgcgg gctggtggaa gtatgcatgt tgaaattctc gcggaagaga tgttcgttga
      241 atcccgggtc attgacatga atcatgctgg attgcgagaa agccgataac accttgtgtg
      301 tgctctgggt agcgtaatcc gcccccgcct ccattgccgt cggccggaag gccgggtgaa
      361 aacgggcgaa agcgtaccag gcttcgtcaa tgatcacctt tatgcctttc gcatgggccg
      421 cctcgatgat cgggggtaga tcgtagcgca ggccatcgta tgtgcaactc gtcagtatca
      481 ttgcctgcgc gtccgggtgt tcctctatgg ccgtaaaaag ggtctgtttc ggtccgggcc
      541 cataaatact gaatttcttg ttgactgaag aatcgagata gatcggatac gcgcccgaga
      601 gcactacccc gtgatgcacc gatttatggc agttccggtc cagtatcagc ttctcgcccg
      661 gcgccaacag ggtctgaaaa atcaccttgt tggccgtgga cgtgccgttg gtcgcaaaga
      721 aagtacgacg cgcgccaaaa gcctttgcgg caatcttctg ggcttccgct atcacccctg
      781 taggctccat gagggaatcg agcatgggca cagagacaga caaatccgcc cggaagacat
      841 gatcgccaac gaaatggtaa aagtcgctgg cccacgggct atcgcggacc gaatcgcccg
      901 aagaatgccc cggtgtatgc catgcatcct tggccatcaa tacgtaattc ttgagctgat
      961 cgtagaatgg cgtccgcgcc ttttcctgca actgggcatt caggatgcga tacatgccgc
     1021 ggtagtcacg ttcttcccga tagaagtaac cgtcaaccgt ttcggaaaag agagcgtcca
     1081 ccacttcatc ctctttttcc tgggcaatca ggatgtagat atcgagctca gggcgaaaac
     1141 gattgatttc ctgcaccagc ttcaacgccg tcatttgcag gtttcgggcc cccttttctc
     1201 cattttttag agtgtagagg gtatcatcca cgaccaccga ctgaatatcg ccatcttcct
     1261 ggatggcctg caacgcttct cttgctgtag tgacgcctga aaatgtgatg ccaagcggat
     1321 tttccagcga acgggcggct gaattcagcc ccttgatgag ctccttcagt atcagttttt
     1381 catcattgat cacaagaatc cggctgatcg gcttcttcat aatccaaagc tcaaaaaaaa
     1441 gacgtgtcgg gagtaaaagc ttcgctcgct cgcaacctgg aacgcgataa cgcatggtca
     1501 cggcaaatgc gaaccgatat tcgggcttgc ctcagcaaaa tcaaaacctg ccaatccaaa
     1561 accggaaaca aaaaaagccc ggcaacccgg gctttttcct gataccagcg ataatgctca
     1621 agccatcgcc ttgatggccg cgacaaggcg gcttttgtga cgtgctgctt tattcttgtg
     1681 aatgatcccc ttatcggcaa tcgaatcgat cgtaccgacc gaagcctgaa aggccgcttg
     1741 cgcggtggcc ttatccgccg atgcgatgcc ttttctcaca tgcttgatcg cagtccgaag
     1801 ctgcgaacgc aggctcatgt tgcggtggtg gtgacttatg ttctgtctgg cgcgtttcct
     1861 ggcctgggca ctgttagcca ttaaaatccc tcatggatga gctttattgc aaatcttaaa
     1921 ccgcgaaatg atacggatat tttatgcgtc ttgcaatgaa aacattctga attcccaaag
     1981 ctcagctggt aaatgagttg gtaaaatgct tcgtgcccgc acccttggtg tcgaccgtcg
     2041 atattccaac ccgtggcctg gcatgcccca ttactttcta tcgttgccct ccaccatgaa
     2101 tttgctcaaa acactggcca cggtcagcag catgacgttg atctcgcgca ttctcggatt
     2161 tgtgcgtgac atgctggtcg cccgcgtttt tggagcaagc gttgccactg atgctttctt
     2221 tgtggctttt cgaataccca acctgttgcg ccgcctcttc gccgaggggg cattctccca
     2281 ggcctttgta cctattctat cggagtacaa aaacaagcgg actgaagatc agacacgcga
     2341 actgatcaat aaagtctcgg ctctcctgag catagcctta ttcacgatta cgctcatcgg
     2401 ggtcattgcc gcccccattg tcatatacat cagcgcgccg ggtttttcag cgagtccgca
     2461 gaaattcgga ttaacggtcg acctgctgcg tatcaccttc ccctacatcc tgttcatctc
     2521 gctggtatcg ctggcgggag ggatccttaa tacctatgga aagttcagca ttccggctct
     2581 gaccccggca ctcctgaatg tgtcattcat tggctgcacg ctatggctgg cgcccctgct
     2641 ggatccaccc gtcctggcgc tggcctgggc ggtatttatc ggcggggcgc tccagttggc
     2701 ctttcaggtt cccttcctga tgaaaatcag gctcatgccc tgccctagcc tcaactccga
     2761 cgatcctggc gcctggcgga tcgtcaggca gatggggccg gcagtgtttg gtgtctccat
     2821 cagtcagatc agtctgctca tcaatacaat ttttgcttcc tttcttgtta cgggaagcgt
     2881 ttcatggctt tattacgcag accggctgat ggaatttccc gcaggtctgt tgggcgtggc
     2941 gcttggtacc atattactcc cgtctctatc ccgtcattac gcacaaaaca gtatggaaaa
     3001 atactcccgc ctgctcgact ggggcttgcg cctgaccatg ctgctcaccg ttccggcggc
     3061 gctggctctg gctttacttg ccactcctct tgttgccacc ctctttttcc atggccagtt
     3121 ttccccgaat gatgtatgga tgacccgcga cgcagtggtc gcttacagcg ttggtctcct
     3181 gggattgatc ctggtcaaag tgctcgcccc gggattttat gcacgtcaaa atatcaaaac
     3241 ccccgtcaaa atagccattc tcacgcttgt aatgacgcaa atgatgaatc ttgcattcat
     3301 cgtaccgctc cggcatgtcg gcctcgctct cgcaatcgga ctgggcgcct gcctcaacgc
     3361 cgccctcctt tactacaagt tgcgcagcca gaatattttc cagccacagc ccgggtgggc
     3421 aatcttcata gctaaaatat tgactgcgtc gagcggcatg ggcgttctgt tgtgggttgt
     3481 ttccggcagc gatgcatcat ggctggcaga ctccacgttc gaccgggtgc ttcgcctggt
     3541 ctatattgtt gcgctgggtg gggcaagcta ttttgcaatg ctatgggttc ttggattccg
     3601 cctcagtaac ttttcgaaac atgcggccgg gactgatatc ggggataagg aataatcttc
     3661 ctctgaataa cagctcatca atggacagct tttatctgtc gggaaaactg cgattcccct
     3721 ggtacattcg ttgaaacaac aggggaaata gacttttatt gacggcctaa agccaccccg
     3781 ggcttgcaaa aaaagttcat tttgagattc gaatagacct ataatggcgg cggtcattcc
     3841 cgctaacagc aaacaaccga taccgatata catatgactc tttctgtcag actctcaacc
     3901 ttatccgtat tgctcctggg atttctattg aacggctgcg ccagtaccag caaaa
//
LOCUS       DASSHJ010000237         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1547166,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000237 DASSHJ010000000
VERSION     DASSHJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547166"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFI43_09225"
     CDS             complement(<1..295)
                     /locus_tag="VFI43_09225"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HET7062345.1"
                     /translation="MKSPLAEVQGVMDTRRIPINRAGIRAIRHPVKIADKYGTVQHTI
                     GSFNMSVSLAHQFKGVHMSRFVELLNDRDEELSIHSFEHMTRAMIEKLEADCGY"
     gene            complement(350..>2048)
                     /gene="dxs"
                     /locus_tag="VFI43_09230"
     CDS             complement(350..>2048)
                     /gene="dxs"
                     /locus_tag="VFI43_09230"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET7062346.1"
                     /translation="LTIALHYVFNTPHDRLVWDVGHQTYAHKILTGRREGMSKLRMQG
                     GIAGFPRRDESEYDAFGTAHSSTSISAALGMAVASQQEGADRCAIAVIGDGAMSAGMA
                     FEALNNAGAMDANLLVILNDNDMSISRPVGALNNYLARLMSGQFYATARRAGEKMLGV
                     VPPVLELAKRAEEHVKGMVTPSTLFEEFGFNYIGPIDGHDLDVLVTTLNNIKHLRGPQ
                     FLHVVTRKGAGYKAAEEDPILYHGVTKFNPSAGIVPKTGGKPPYTQIFGDWLCDMAEL
                     DQRLVGITPAMREGSGLVRFSQEYPDRYFDVGIAEQHAVTFAAGLACDGMKPVVAIYS
                     TFLQRAYDQLIHDVAIQNLPVVFAIDRAGLVGADGPTHAGSFDLTYLRCIPNMTVMAP
                     SDENECRQMLYTAFQLDTPTAVRYPRGVGAGIVPQKQMQMLPVGRGEIRRDGSKAALL
                     AFGSMLKPCLDAAEELDATVVNMRFIKPLDDDLIASLAQKHPLLVTVEENTIMGGAGS
                     AVLESLASRGICVPVLQLGLPDVFLDQGDPSRMLADCGLDKEGIVKSVQARLSQQLA"
BASE COUNT          447 a          596 c          537 g          468 t
ORIGIN      
        1 gatatccgca atccgcttcg agcttttcaa tcatcgccct tgtcatatgc tcgaaggaat
       61 ggatcgacaa ctcctcatcg cggtcattga gaagctccac aaagcgggac atgtgcaccc
      121 ccttgaattg gtgagccagg cttacgctca tattgaagct tccgatcgta tgctggactg
      181 tgccgtactt gtccgctatt ttaacggggt ggcggatagc tcttatacct gccctgttga
      241 tcgggatgcg gcgcgtgtcc attacgcctt gaacctctgc aagaggcgac ttcataccgt
      301 tcattgcggt actgttcttg taaccgggca acagcgcatg gtaatcctgt caggcgagtt
      361 gctgcgacag ccgggcttga accgatttga caatgccctc tttatccagc ccgcaatccg
      421 ccagcattcg tgaaggatcc ccttgatcca agaaaacgtc gggcaaaccc agttggagca
      481 cgggaacaca gattcctctg cttgcaaggg actctagtac cgcgctgccc gcgccgccca
      541 ttatcgtatt ttcttcgaca gttaccaata acgggtgttt ttgtgccagc gacgcgatca
      601 agtcgtcatc gaggggtttg ataaagcgca tattaaccac agtagcgtcc aattcctctg
      661 cggcatcaag gcagggcttg agcatgcttc caaaagcgag gagcgcggct ttcgacccat
      721 cacggcggat ctcgccgcgc ccgaccggca gcatctgcat ctgtttttga ggcacgattc
      781 ctgcgccgac accgcgcgga tagcgcacgg cagtaggcgt atccagttga aacgccgtat
      841 agagcatctg gcggcactca ttctcgtcgg aaggcgccat gactgtcatg ttcgggatgc
      901 accgcaagta ggtcaaatcg aagctgcctg catgcgtagg gccatccgca ccaacgagcc
      961 cggctcgatc gatcgcgaat actacgggca gattctggat cgcaacatca tgaatcagtt
     1021 gatcataggc gcgttgcaag aacgtggaat agattgccac cacgggtttc atgccatcgc
     1081 aggcaagccc tgcggcaaat gtaacagcgt gttgctccgc tatacccaca tcaaagtaac
     1141 ggtccgggta ttcctgcgaa aatcttacca ggcccgatcc ctcgcgcatt gcaggagtaa
     1201 tacctaccag acgctgatcc agctcagcca tatcgcacaa ccagtcccca aaaatctggg
     1261 tataaggcgg ctttccccct gtcttgggga cgatgccggc ggatggattg aacttggtaa
     1321 cgccatgata aagtatcgga tcctcctctg ccgctttata gcctgcgcct ttgcgcgtga
     1381 cgacgtgaag aaactggggg ccgcgaagat gcttgatgtt gttgagcgtg gtgaccagca
     1441 cgtcaagatc atggccgtct atcggcccta tatagttgaa gccgaattct tcaaataatg
     1501 tgctgggtgt taccatgccc ttgacatgtt cttccgcacg cttggcaagt tccaggacgg
     1561 gaggaacgac accgagcatt ttttcgcctg cacgcctcgc cgttgcataa aattgtcccg
     1621 acatcaatct ggcaaggtaa ttgtttaacg cgccgaccgg ccgggaaatg gacatgtcgt
     1681 tatcgttcag gatgacgagc agattcgcgt ccatggcccc ggcattattc agggcttcga
     1741 acgccatccc ggcgctcatc gctccatcgc ctatcaccgc gatcgcgcag cggtccgcac
     1801 cttcctgctg cgaggccacc gccatcccaa gggccgcgct gatcgaagta ctggagtggg
     1861 ctgttccaaa cgcgtcatac tcgctttcgt cccttctcgg gaatccggca atccctccct
     1921 gcatacgcaa tttgctcata ccttcccggc ggccggtcag aatcttgtgc gcataagtct
     1981 ggtgtccgac atcccatacc aagcgatcgt gcggggtgtt gaaaacgtaa tgcaaggcaa
     2041 tcgtcagc
//
LOCUS       DASSHJ010000238         4486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_694267, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000238 DASSHJ010000000
VERSION     DASSHJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694267"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFI43_09235"
     CDS             complement(<1..366)
                     /locus_tag="VFI43_09235"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET7062347.1"
                     /translation="MSAADSRQYRWHKYPDKAALNDAAVGAILDSAACSIRERGRFSI
                     VLAGGETPREIYMRMRSAPTDWSLWHIYFSDERCMPPTEDELNSHMAGEAWLDHVPIP
                     TEQIHVIPNGPRADKAAAEY"
     gene            complement(446..871)
                     /locus_tag="VFI43_09240"
     CDS             complement(446..871)
                     /locus_tag="VFI43_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7062348.1"
                     /translation="MELGVKAMLNLIRNDNLGKLILRLTVGILILFHGIYKLLHPESL
                     QGIRKMLTGIGLPELLAFGVFLGEVIGPLMVIVGFYSRIGGLLIFGNMIFALVLAHRA
                     QLFTLSSTGGWALELQAFYLFCSLAVLFLGSGRLALKPD"
     gene            complement(1111..1551)
                     /locus_tag="VFI43_09245"
     CDS             complement(1111..1551)
                     /locus_tag="VFI43_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062349.1"
                     /translation="MKARHIVTALSLFSLSALVNPAAIGSELGDLHILQAAQKATSRS
                     DHEAIATYYEDAAKAMHLKMQEKKQLLEHYQDKSYLYGRQAQDLQSHTEALARQYEGA
                     VKKNVKEAQLHHQMALNVEESPVAESANQKLSAVQSHHNRFTAE"
     gene            1726..2040
                     /locus_tag="VFI43_09250"
     CDS             1726..2040
                     /locus_tag="VFI43_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062350.1"
                     /translation="MTFVNFASAKAVELLLLNATPLTVFNLWNLKKHEPCDNNITKIL
                     EVEKNVQPSGFGLHKSGETRGCIEGSARQFAVSDFLDDRVLTMIFSSMMFSLPIDVFI
                     LE"
     gene            complement(2209..3072)
                     /locus_tag="VFI43_09255"
     CDS             complement(2209..3072)
                     /locus_tag="VFI43_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7062351.1"
                     /translation="MATIYGTNFNDNDTWQGFWFYPSLRGSNYADAIYGYSGNDILYG
                     YNGNDYLDAGSGADIAYGGDGNDTIWGAISTVTWDLGDNLFGEGGNDLIGGSYGSDYI
                     GGGTGDDSLYGDEGNDSVYGGDGNDLLRGDWGWGSDLYGNDYLNGGNGNDKLYGGSGN
                     DWLYGGDSNDVLTGGLGKDFLSGGNGSDVFDFDSTSESPWWARDTISDFSGARWQWDP
                     WIGFHLVAGDKIDLSTIDANSFVSGNQAFNSTQLSFNSGVFTAHVYGGDDVQINLTGT
                     PTFNINLDVIV"
     gene            complement(3406..3624)
                     /locus_tag="VFI43_09260"
     CDS             complement(3406..3624)
                     /locus_tag="VFI43_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062352.1"
                     /translation="MRLLQDERYGSSRLSRKALTCVFQRAVELLVPFGENQSLLSVVD
                     ALAASIPHSGSQANPSKLQDAVERSVSP"
     gene            complement(3670..>4486)
                     /gene="gnd"
                     /locus_tag="VFI43_09265"
     CDS             complement(3670..>4486)
                     /gene="gnd"
                     /locus_tag="VFI43_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET7062353.1"
                     /translation="RSLGGLAQLEKNGGVAASSLEDLVARVKAPRAVWLMIPAGAPVD
                     DIIARLQPLLQSDDVIIDGGNSNYKDTRRRSHMLAGAGIHYVDAGTSGGIWGLKNGYS
                     IMIGGDEKVVDRLRPIFEALAPAPDKGWGRVGPSGAGHFTKMVHNGIEYGMMQAYGEG
                     FAIMRHKEEFNLDLHQVAEIWRYGSVVQSWLLDLAAAALAQNPHLDNIAPHVADSGEG
                     RWTVAEAIDLDVAAPVITLSLLQRLQSRDSESFSNRLLSALRNQFGGHEMKKQ"
BASE COUNT         1098 a         1151 c         1128 g         1109 t
ORIGIN      
        1 gtattcggcg gctgctttgt ccgcccgtgg gccatttgga atgacgtgga tctgctccgt
       61 gggaatgggg acatggtcga gccatgcttc gcctgccatg tgcgaattca actcgtcttc
      121 agtcggaggc atgcagcgtt catcagaaaa atatatatgc cacagcgacc agtcggtcgg
      181 cgcagatcgc atcctcatat aaatctcgcg cggcgtttcg ccgccggcga gaacaatgct
      241 gaaacggcca cgttcccgaa tggagcatgc ggcactatcg aggatggcgc ccaccgctgc
      301 atcattcagg gcagctttat caggatattt gtgccagcgg tattgccggg aatcggctgc
      361 gctcatggaa ggtaaacgga atacatggga agcgttatcc ttatcttttg tttttaatat
      421 tcttatggcg gtctgtctgc cggcatcaat caggtttgag tgccagacgg ccgcttccga
      481 ggaataatac cgcaagactg caaaataagt aaaacgcttg caattccaat gcccatcctc
      541 ctgtcgagct cagcgtgaat aactgggccc ggtgagcgag cacgagggcg aatatcatgt
      601 tgccgaaaat aaggaggccg ccgatacgcg aataaaaccc gacgataacc atgagcggtc
      661 caatcacttc acctagaaaa acgccaaaag caagaagctc gggtaagccg atacccgtga
      721 gcatcttgcg gattccttgg agtgactcag gatgaagcag tttgtatatg ccgtgaaaca
      781 gtatcagaat gccgacggtg aggcggagga tgagcttgcc gaggttgtcg ttccggatca
      841 ggttaagcat tgcctttacc cccagttcca tgagcctcgc acgacaagca gcaggctgct
      901 tttagccacg cggaatcaga ttatccttcc cagctttcag tctagaagaa attacttttt
      961 gattccggat tttctcctag tcttgctata gccaaggcgg gctcatgggg aaagagcaga
     1021 ggaacttcgt ttatcccttg ggcaaaggga ctgctgggct ggcaaaagtt cagctaaagg
     1081 caatgcgagt cctaccattg ccttgatccg tcattcagcc gtaaagcgat tgtgatggga
     1141 ctggacagcg ctcagtttct gattggcaga ttcagcgaca gggctttcct cgacattaag
     1201 cgccatttga tggtggagct gtgcttcctt gacgtttttt ttcacggccc cttcgtattg
     1261 gcgagccaac gcctcggtat gcgactgaag atcctgggcc tgccgaccgt agagatagct
     1321 tttgtcctgg taatgttcaa gcagctgctt tttctcctgc atttttaaat gcattgcttt
     1381 tgctgcatct tcgtaatacg tagctattgc ttcatgatcg ctccgcgagg ttgccttttg
     1441 ggcagcttga agaatatgga ggtcgccaag ctcgctccct attgccgcgg ggttgacgag
     1501 tgcggacaag ctaaacaagg ataacgctgt aactatatgc cttgctttca ttgcgacttc
     1561 tccttttcat gtcgccaagc ggcgacggga aatcaggtgt tatcaggtgt cttaaacgcc
     1621 attccctaag tttgcggaga gaatgtgaaa tttcatgtat gtggtataaa gaatacgtca
     1681 ggagcaccac tttgtcaagt ttattatgtg taaagtgaat tcaaggtgac attcgtcaac
     1741 tttgcctcgg ccaaagcggt cgaactgctg ctccttaacg ctacgcctct aacggtattt
     1801 aatttatgga atttaaagaa gcatgaaccg tgcgataaca atattacaaa gatacttgag
     1861 gtcgaaaaaa acgttcaacc ttcaggcttc ggacttcata aatcaggaga aacgcgtggg
     1921 tgtattgaag gttcggcgcg tcaatttgca gtgtcagatt ttttggacga cagagttctc
     1981 accatgatct tttcatcaat gatgttctca cttcccattg atgtttttat tttggaatga
     2041 cgcgacgata tgggcgtgct gcaagcaatc gccaaataga aaaagaccga ctaagttcaa
     2101 caagacgaac agggctttgg caaatctgca agaattgacc tgcatgtagc atgtacgttc
     2161 gcacgtacat gcaggccaag aaccttacgt ttgaagtgcc atcaatattt atacgatgac
     2221 atccaaatta atgttgaatg tgggagtgcc cgttaaattt atttgaacgt catcgccacc
     2281 gtacacatga gcagtgaaca ctccactatt gaacgacagc tgcgttgaat tgaatgcctg
     2341 attgccggaa acgaacgaat tggcgtcaat cgttgaaagg tcgattttgt ctcctgctac
     2401 cagatggaaa cctatccatg gatcccattg ccatctcgcc ccgctaaaat cagagatggt
     2461 gtctcttgcc caccaagggc tctcagatgt cgagtcgaaa tcgaagacgt ctgatccgtt
     2521 tcctccagaa aggaagtctt ttcctaatcc acctgtcaaa acatcattgc tgtcaccgcc
     2581 ataaagccag tcgtttccac ttccgccgta cagcttgtca ttaccattgc cgccgttcag
     2641 atagtcgtta ccgtaaagat cgctgcccca gccccaatct ccacgcagca ggtcattacc
     2701 gtcgccgcca tatacgctat cgttcccttc atcgccatat agagagtcat cacccgtgcc
     2761 gccgccgata taatccgagc cgtaagagcc gcctatcagg tcatttcctc cctctccgaa
     2821 caggttatcc cccaggtccc aggtaactgt agatatggca ccccaaattg tgtcgttacc
     2881 atctccgccg tatgcgatgt cagcacccga tcctgcatct agataatcat tcccgttata
     2941 accataaaga atatcattcc cggagtatcc gtagatcgca tctgcgtaat tactcccgcg
     3001 taacgatgga tagaaccaaa aaccctgcca tgtatcgtta tcattgaaat tagtgccgta
     3061 aattgttgcc atgatagtct cctcaaaata tacatggata cggctgtaaa ccatcgggcc
     3121 gtaaattcac gatacgcggc gacgcaggaa aagttgtaaa ggattggtta aatatcggtt
     3181 atattttggt taaattgccg gcaaaggcgc tggtcgtgga acaacaggcc ggtattagaa
     3241 gaggactgaa cggcttacgg gaaatcgtgc ttcttctctg caagcaggga ggggaaagag
     3301 aattctggca ggtttggata caggttaagc gatttcgcca agattcgcgg gtagcctgaa
     3361 gggttgtcta aattgctgaa gaattgtcca gattgtccac gagggctaag ggcttacgct
     3421 tcgctccacc gcatcctgca gtttcgaagg attcgcttgg cttcccgaat gtggaataga
     3481 agccgctaag gcatctacaa cggagagcag actctggttt tcgccgaaag gcacgagaag
     3541 ctctacggca cgctgaaaga cacaggtcag cgccttcctg gacaagcggg aacttccgta
     3601 gcgctcatcc tgaagcagcc gcattcgaat ctcctcgtcc gcgaaaacgg atgagctatc
     3661 agcaggagat tactgctttt tcatctcatg gccgccaaac tggtttcgca aggcggacaa
     3721 caggcggtta gagaatgatt cggaatcgcg ggactgcaat ctttgcaaca gcgagagggt
     3781 gatgacaggg gcggcaacgt ccagatcaat cgcttcggcg acagtccagc gcccttcacc
     3841 cgaatcggcc acgtgcgggg cgatattgtc taaatgtgga ttttgcgcga gggcggcggc
     3901 agcaagatcg agcagccacg attgaacaac gctgccgtat cgccagatct cggccacttg
     3961 gtgaaggtcg agattgaact cctctttatg gcgcatgata gcgaaacctt cgccataagc
     4021 ttgcatcatt ccgtattcga taccgttgtg aaccatcttc gtgaagtggc ctgccccgct
     4081 gggcccgacc cgtccccagc ctttatccgg ggcaggcgcc agagcttcaa atatcggacg
     4141 caggcggtca acaacttttt catctccgcc aatcataatg ctgtagccgt ttttcaagcc
     4201 ccatatcccg ccactggtgc ccgcatccac atagtggatc cccgctcccg ccagcatgtg
     4261 cgaccgccgc cgggtgtcct tgtaattcga gttgccgccg tcgatgatga catcgtcaga
     4321 ctggagcagt ggttgcagcc gcgcgatgat gtcgtcgaca ggagcacctg cgggaatcat
     4381 cagccatact gcgcgaggcg ccttcactcg cgcaaccaga tcttccaacg atgatgcggc
     4441 aacgcctcca tttttctcga gttgcgcgag tccacccagg cttcgg
//
LOCUS       DASSHJ010000239        13863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1219878,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000239 DASSHJ010000000
VERSION     DASSHJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 13863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13863
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219878"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1056)
                     /locus_tag="VFI43_09270"
     CDS             complement(118..1056)
                     /locus_tag="VFI43_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HET7062354.1"
                     /translation="MPGAYAHLTLVNLIREPGRLESRGFVPDAIVPLLDYFRFCELGA
                     VSPDYPYLDITHPDACHWADRMHYQKTGDMVKAGIDRIKKLDGEARQKTFAWLLGYTS
                     HVVTDVTIHPVIELKVGEYQKNKDKHRICEMHQDAYIFQRLNLGEIGLAEHLDSGIWG
                     CCDAPQSGKLDPTIKNTWEGMLEACYSEPYQTRPPVIDNWHGSFRFIVDKAEEGGVLP
                     AFARHVAANIGLTYPALDDLDNQYLKGLATPLGAMSYDEIFDRAMENVLDAWFHVAGA
                     VFKDDDTYQTAFTNWNLDTGRDPAGSYAYWQERGAA"
     gene            1246..3126
                     /locus_tag="VFI43_09275"
     CDS             1246..3126
                     /locus_tag="VFI43_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7062355.1"
                     /translation="MFNFFERLVLPYPDSPVKIPPKGFFAFAWEATEGVRRYLVAMTL
                     LTATIGAFEALWFAILGKVVDWLNQIPPSLLWEQEKATLLFFAALLAASPLLVGFQNL
                     FKHQVLGGNFPMRLRWNFHRLMLNQSMNFYQDEFAGRVAAKVMQTSLALRDVCFILGD
                     ILVYVTIYFFTLVAVVGNFNIWMSLPFIGWFVLYILALRFFVPRLSRVSESQADARAL
                     MTGRISDAYTNIATVKLFSHAGREAGYARSAMRDFLKTVHNQMRLITGFEITNQALGA
                     SLIICAVGLALFLWTEGQIGAGALAAVGAMALRLNGMSHWVMWEMASLFEQVGTVRDG
                     INTLSRIHLVTDHPDAKPLQVKRADISFDDVFFAYGGSVPVIDHLSLHIRPGEKIGLV
                     GRSGAGKSTLVNLLLRFFDIEQGRILIDQQDIRYVTQNSLRANIGMVTQDTSLLHRSV
                     RDNILYGRPDATDADMVAAAKKAEAHEFILDLTDAMGRRGYDAHVGERGIKLSGGQRQ
                     RIAIARVMVKDAPILLLDEATSALDSEVETAIQSSLYKLMEGKTVIAIAHRLSTIAAM
                     DRLIVVEKGCIVEEGSHQSLIALNGIYARLWTHQSGGFLGDDVEVRAEGMRVVAASGV
                     GK"
     gene            3407..4036
                     /locus_tag="VFI43_09280"
     CDS             3407..4036
                     /locus_tag="VFI43_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET7062356.1"
                     /translation="MKKKTLWYVADPMCSWCWGFAPVIERIRKEYGDRLKFELLLGGL
                     RPGTTHPISPEQRQEILHHWENVHRLTGQPFQFAGAMPEVFVYDTEPASRAVVAALLL
                     VPEVGFPFLKAVQHAFYAEQQDVTKTAVLVQLADQAGLDAQQFAATFESHAAKKLTLD
                     HFTRARHLGIGGFPTLLLEDHAGYRALTRGYIPFQHLSLKLEKWLGLQP"
     gene            4180..4752
                     /locus_tag="VFI43_09285"
     CDS             4180..4752
                     /locus_tag="VFI43_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HET7062357.1"
                     /translation="MPKRASTVYFLWILISLISPIETPAEPPLWVGNHNGGENGHGKG
                     KKLRNGNVNKIDQGYPGNSRGHLNYTGNGIGYGGGPYSTRNTVRSFDDHQHRLISNYF
                     GPRFRNGHCPPGLSKKHNGCMPPGQVKQWRIGYPLPSNVVFYNLPAALLGQLGHPGPG
                     YRYVRIAGDVLLIAAATGLVMDAIQDLNSL"
     gene            5212..5850
                     /locus_tag="VFI43_09290"
     CDS             5212..5850
                     /locus_tag="VFI43_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7062358.1"
                     /translation="MKTLSTCFIGVLAFTGIITNAAAAGVITSVSGAGGGLGSFGVLN
                     HAIVSKQLNLIKTFDHIDPIDLTFTVIHGQGAGGSYSVTETITNHTGLPWGDFHFVIS
                     EPAEGSQGMVFTNFQSASLAGFTLDSPPSSGPRELNFTGNLASGASATATFSLNLPDP
                     GAGKTLTFTLTQKPSLETSVSPVPEPETYGMLMAGLGIICLVVKRRSSKQSV"
     gene            6158..6871
                     /locus_tag="VFI43_09295"
     CDS             6158..6871
                     /locus_tag="VFI43_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7062359.1"
                     /translation="MTGSNIPGRNKLTARVLAGFGMLAASLSAHALVIDINSYVTGSG
                     ATLNTTVATLTLTQNGSNVDFSFNNLVNNLSSGVGDDAFISELLFSYDRMPSLTTASF
                     SNFGGSQPITASAFAISPPGKDAGYDFYLALSYPTSTGNRFTHGEFSTWTISNVVIAD
                     FLQPVSGSGPASLAMAHIQQVGAGPGGVGSVKYAGSDNSDPPQLAENPVPEPGSLALL
                     LAGLVGFGAIYRRPSRTTL"
     gene            complement(6866..7537)
                     /locus_tag="VFI43_09300"
     CDS             complement(6866..7537)
                     /locus_tag="VFI43_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062360.1"
                     /translation="MLGSRLEAIDADCERAAKIELAQLVENAKDSENNLRRPSPQAAE
                     AVWLKLISRKENEFIQEIDKSLKGKSGEQSVAKTAPVDNEDIRHIEAIVGELLADDRY
                     VDRMQDFYKEATPEVSEPEAGTAGGAKQAGFIDRTYRTGITAALLKARRNVLYELESH
                     KPLEPEDASFSSQWKRYSTLSPWRLIWTIVLLSLTSYLIAFIIASDAFRGFLERMGWS
                     VGTGL"
     gene            complement(7723..8793)
                     /locus_tag="VFI43_09305"
     CDS             complement(7723..8793)
                     /locus_tag="VFI43_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7062361.1"
                     /translation="MKYDVLVRRPNDLTVEWAQRVVNSRYSGIRISAVHIVSVDIGTT
                     TRVRLRVEHDGPNDMPRRWFVKLPSLAWRARIITALPRLLHTEVRFYREAAQIVPTAV
                     PDFLAAQSERGRGAVLVLRDVTECGATPGSPGDTLTPGQAALVVEELARFHAHFWGKA
                     ELLQTFGWLAGPVRRLEDGLSATLAVPLMKRGLRRAGQAVPSALHALAVHYARRRSTI
                     MRFLSSAPQTLVHHDCHPGNLFWHKYRPGFLDWQLVRFGEGIGDVAYFLATSLNPETR
                     RIHEASLVSAYAAALQRHGVPGIEPDNLMQRYRAHLIYPFEAMVITLAVGGMMKPENN
                     YELIRRATAAIEDRDAFGAVMI"
     gene            complement(8955..9323)
                     /locus_tag="VFI43_09310"
     CDS             complement(8955..9323)
                     /locus_tag="VFI43_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HET7062362.1"
                     /translation="MKNMLKPLLVVAALLFFSPQASMAEGSYPQHVGEKLAHGIANTV
                     TGIGEVPKNMMIESREKGPAYGVPVGFIVGIVQGLGRTLTGVVDLVTFVIPTKPIIYP
                     DFIWKDWYKETHYHPEWKLQ"
     gene            complement(9925..11448)
                     /locus_tag="VFI43_09315"
     CDS             complement(9925..11448)
                     /locus_tag="VFI43_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HET7062363.1"
                     /translation="MNILIVVDEPADWPLDIPDVRIVAAQAYLTDPLHADGASRARGR
                     AASRSVKIFNLCKSYQYQSSGYYVSLLAEARGHKPLPKVGTMEDLQSQNLVRYLTEDL
                     GPLIQRLLAPQDMNNFILGIYFGQSIDKRYDSLSQQLFDRLQAPLLSAHFERHDDQWI
                     LRSVCATTVSDIPPEQREFAVRAAVGYFTGRNHSARKRPPRFDLAILLNPDNPEPPSN
                     SKALQKFRKAAEDLRMRVEFITGADAGRLPQFDALFIRDTTFVNHYTYRFSRRALAER
                     LIVIDDPDSILKCNNKVYLAELLSQHNIRTPKTLLVHRGNISWIIPELGLPCILKQPD
                     SSFSRGVAKVKTEEELNAKIPELLGKSALIIAQEWLPTEFDWRVGILDRRPLFVARYR
                     FPTGHWQVIKRDAQQHKLSEGATEAVALDQAPEQLIAVALRSANLIGDGFYGVDLKQV
                     GNRFYVIEINDNPNVDAGNEDAVLKDALYREVMGVFLKRIEARKRGDSWPPHLPLYH"
     gene            complement(11501..12937)
                     /locus_tag="VFI43_09320"
     CDS             complement(11501..12937)
                     /locus_tag="VFI43_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062364.1"
                     /translation="MFGSSILEVAIGVTFVYLLLSLICSALNEAIASLLQKRGRNLFE
                     GVKNLLNDPTFTGLAQQVYNHGLIGGISQYASNPAKPTRPPSYMSPENFSLALLDILG
                     IRGAIAKEYGDLLAQAESADDAYEEARKAAAAGSGNPQLAEALAAARNKQEQARAALD
                     ALADKARISYEAALKNSNEAPGNLELAKLAVDAKVSLGGVTAALQMLEARRAAIASAK
                     NPKEMALALNAGATLRKALEFGRTIAAQYPDPLGNIQDGLMRLPEGHTRESLLVLVDK
                     TRREVSAVEHQAEAFRRNIELWFHDAMNRVSGWYKRWTQHVLLVLATLVVLVSNADTV
                     MLVQRLSQDNALRASLVAAAQEAAKAVSSPGTSNPGSSEPLPLAEDARIQLVIEKARS
                     LQLPIGWSLDPQDPGYFRSPEISLPFAGWVLYKLFGLLITIFAVSLGAPFWFDMLSKV
                     VNVRSAGTPPGESKKGSPQKSDGQPGAK"
     gene            13069..13812
                     /locus_tag="VFI43_09325"
     CDS             13069..13812
                     /locus_tag="VFI43_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HET7062365.1"
                     /translation="MRRISVALLLFAAAGFASVASSANIPGFAGASVDVPDTIAERVK
                     PCVICHKQGDQTDVNGYYPRIAGKPDGYLYNQLRNFRDGRRYYRPMALLLENLSDEYL
                     MEMARYFSLLKQPYPPPERVVLPPEEIKLVRKLIEEGDPSRRIPPCRECHGKELMGTK
                     PFIPGLLGLPRSYMIAQFGAWRSGGLMRRQSANCMSEIARQLTPQESNAIAAWLAAQP
                     IPQNAEPGTGLSDEMARRCGIDGQREAVR"
BASE COUNT         3078 a         3711 c         3753 g         3321 t
ORIGIN      
        1 ataatttcgt taatgctgac gctttgacgt acatttcttc gctgtccgga tgccgaggtg
       61 agcgagcgca tccggcaatg aaagcaagcg caagcagtgc cataatgatc ggtctgctca
      121 tgctgcgcct ctctcttgcc aataggcgta gctgcccgct ggatccctcc ccgtatcgag
      181 attccagttg gtgaaggcgg tctggtaggt atcgtcgtcc ttgaagacgg caccagcaac
      241 gtggaaccag gcgtcaagga cgttttccat ggcccgatcg aatatttcgt cataactcat
      301 tgcacctaaa ggcgtagcca atcccttcag atattgattg tcgagatcgt cgagggcggg
      361 gtatgtcaga ccgatattcg ctgctacatg tcgcgcaaac gctggcagga ctccgccttc
      421 ttccgcttta tcgacaatga acctgaatga gccatgccaa ttatcgataa caggagggcg
      481 ggtttgatat ggctcgctgt agcaggcttc gagcatgcct tcccaggtat ttttgatcgt
      541 gggatcgagc ttcccgctct gcggcgcgtc gcagcaaccc cagatcccgg aatccaggtg
      601 ttcagccaag ccgatttcgc ccaggttcaa tctctggaat atataggcat cctgatgcat
      661 ttcgcatatc cggtgcttat ccttgttctt ttggtattca cccaccttca attcgatgac
      721 cgggtgaatg gtcacatcgg ttaccacatg gctggtgtat cctagcagcc aggcgaaggt
      781 tttttgccgt gcttcgccat ccagcttttt tatcctgtcg attcccgctt tgaccatgtc
      841 tcccgttttc tggtaatgca tgcgatcggc ccaatgacag gcatctggat gcgtaatatc
      901 gaggtatgga tagtcggggc tgaccgctcc gagttcacaa aagcggaaat agtcgagcag
      961 cgggacaatt gcgtccggaa cgaaaccacg actttcgagc cggccgggtt ccctgatcag
     1021 gttgacaagg gtcaggtgtg cgtaggcgcc gggcatttgt atttttacag gtaaattgtt
     1081 ctattgagga tttatcgcaa tttttaaccg atccagcgag tgccatgcgg ttttgacaat
     1141 gctattgtat attttaccgc gctttcggcg cccgctcgat ttgcttatgg cctcaacgac
     1201 gagaacgttc ccggttaccg ataacttact gacttctgaa tagcattgtt taattttttc
     1261 gaacggctgg ttttaccata cccggactca ccggtaaaaa ttccaccgaa aggttttttt
     1321 gctttcgcat gggaagcgac cgagggggtg aggcggtatc tcgttgcgat gacattgctc
     1381 acagcgacta tcggcgcgtt cgaggcttta tggttcgcca tccttggcaa agtcgtcgac
     1441 tggcttaacc agattcctcc atccctgcta tgggagcagg agaaggccac gcttttattc
     1501 tttgcagccc tgctggcggc gtctccgctg ctggtcggat tccaaaatct gttcaagcac
     1561 caggtcttgg gcggtaattt tccaatgcgt ctccgctgga attttcaccg gctgatgctt
     1621 aaccagagca tgaatttcta ccaggacgaa tttgccgggc gggtagcggc gaaggtcatg
     1681 caaacttcgc ttgccctaag ggatgtctgt tttatcctgg gagacattct tgtttacgtg
     1741 accatttatt tcttcaccct ggtggcggtc gtaggtaact tcaatatctg gatgtcgctg
     1801 ccctttatcg gctggtttgt tctctacatc ttggcattgc gatttttcgt gcctcggctc
     1861 agccgtgttt cggaatcaca agctgatgcg agagcgctga tgaccggacg cattagcgat
     1921 gcctatacga atattgctac ggttaagctt ttttcccatg caggcaggga agcgggctat
     1981 gcgcgatcag cgatgcgcga cttcctgaag acagtgcaca accaaatgcg gttgatcacc
     2041 ggttttgaaa ttacgaatca ggctttgggc gcgagcctga tcatctgtgc agttggactg
     2101 gcgctgttcc tgtggacgga agggcaaatc ggcgccgggg ctctggctgc ggtgggagca
     2161 atggcgctga ggctcaacgg catgtcgcat tgggtgatgt gggaaatggc ctcgctgttc
     2221 gagcaggtcg gaaccgtgcg agacggaatc aacacgctct ccagaatcca tcttgttacc
     2281 gaccatccgg acgccaaacc tcttcaggta aagagagcgg atataagctt cgatgacgtg
     2341 tttttcgcat acggaggatc cgttccggtc atcgaccatc tttcgctgca tatccggccc
     2401 ggggaaaaga tcggcctggt ggggcgttcg ggtgcaggca agtccacgct tgtaaacctg
     2461 ctgctcagat tcttcgatat cgagcagggg cgcatcctga tcgaccagca ggacatccgg
     2521 tatgtcacgc agaacagcct gcgcgccaat attggcatgg tgacgcagga tacttcgttg
     2581 ttgcaccgtt cggtgcggga caacatttta tacggccgac ccgacgcaac cgatgcggac
     2641 atggtcgctg ctgccaagaa agccgaagcc cacgaattca tccttgacct gacggacgcg
     2701 atgggacgcc ggggttatga tgcgcatgtc ggtgagcgtg gaataaagct ttccggggga
     2761 cagaggcagc gtatcgccat tgcccgcgtc atggtgaaag acgccccgat acttttgctt
     2821 gacgaggcga ccagcgcgct tgattccgag gttgaaactg ccatccagtc aagcctgtat
     2881 aaattgatgg aaggtaagac tgtaatagcc attgcgcaca ggctgtcgac cattgcagcg
     2941 atggatcggc tgatcgtggt ggagaaaggc tgcattgttg aggaaggaag ccaccagagc
     3001 cttatcgccc tgaacggcat ttacgcccgc ctatggaccc accagagcgg gggttttctc
     3061 ggagacgacg tggaagtacg agctgaaggt atgcgagttg tagcggcgtc tggggtcggg
     3121 aaataagcca ttcttgtgaa catcactatt cgaggcggtg tgcgcggaag gagctgaatt
     3181 gagttttttg ctgcctatat gggcagggca gtttttatcc cgtaacccct ctttacgggt
     3241 gggcagaaca ggtgtttgtt gagcaaataa aggatttgac tctccgctcg attttacatt
     3301 ccatccgtta cggctcttac ggcaggtaac aggtggccgc aggtgcctta acgtggttcc
     3361 agtgctgcca ttcctgagca ggttgtcgat cgcgggggag agcggcatga agaaaaagac
     3421 cttatggtac gtcgccgacc ccatgtgctc atggtgctgg ggttttgcac cggtcatcga
     3481 gagaatccgg aaggaatatg gtgaccggtt aaagttcgag ctattgctcg ggggattgcg
     3541 gccaggaacg acgcatccga tttctcctga gcagcgccag gaaattctcc atcattggga
     3601 gaacgttcac cgtctgactg ggcaaccgtt ccagttcgcg ggggcgatgc cagaagtttt
     3661 tgtctacgac accgagccgg ccagccgcgc cgttgtggcc gcgctcctgc tcgtccccga
     3721 ggtgggcttc cctttcttaa aggcagtgca gcatgcgttt tatgcagaac agcaggatgt
     3781 aacgaaaact gctgtgctgg tgcaattggc cgatcaagcc ggactggatg ctcagcaatt
     3841 tgccgctacc tttgaatccc atgcggcaaa aaaactcacc cttgatcact ttacccgcgc
     3901 acgacatctg ggcatcggcg gttttcccac cctgttactg gaagatcatg cgggttaccg
     3961 cgcgctaacc aggggttaca tcccatttca gcacctaagc ctcaaactgg agaaatggct
     4021 gggattgcag ccttaattcg ggcgaatcca ggcactcgac tggcgttata actcacatcc
     4081 ggatacgccc tcgcgtatgc aacccgttcg ctcgtcattt acagatttaa aaggcgataa
     4141 tttcgcaagc ggtctatttt tcttacacag gtttctttca tgcccaaacg cgcttcaacg
     4201 gtttattttc tgtggatatt aatatcgcta atttcaccca ttgagacgcc ggcggagccg
     4261 cccctttggg tcggcaatca taatggagga gagaatggtc acggcaaagg aaaaaaattg
     4321 cggaacggca acgtcaacaa gattgaccag ggatatccag gcaattcgcg cggacacctg
     4381 aactacacgg ggaacgggat tggctatggc ggaggtccat acagtacccg aaatactgtg
     4441 cgaagctttg atgaccatca gcacaggctg atcagtaatt acttcggacc acgattccgg
     4501 aatggccact gtccgcccgg tctctcaaaa aaacacaacg gttgcatgcc gccggggcag
     4561 gtaaagcaat ggaggatcgg atatccgctg ccatccaacg tggtgtttta caatctgccc
     4621 gctgccctgc ttgggcaact gggtcacccc ggcccgggat accgttatgt gcggatagcg
     4681 ggagacgttc ttctgatcgc agccgctacc ggtctggtca tggacgcaat ccaggatctg
     4741 aactcgcttt gagtcccttg atcattactt gttagaattg ctgcaaaggt tgatctcgag
     4801 caaatcccca ggtttccccc attcttgcgc cgatcatata ccagctgaca ggctatctga
     4861 atctccttct tcagcactgt aaaaatttcc gacaaaaaac tttagggtga ccggcgatct
     4921 gatcgaagcc ccggtcgccg cccgctgttg tttttccaaa ctggatatcg ctaagctctt
     4981 gttttttctc acttcatcga ccggcgtggg gcgcccgtca cttcagtcag ccgatcttcc
     5041 tttttccccg cgctaacatc catctccact tggtaatgat tacatctggc atagtacttg
     5101 cttgtttttt tcggaggcct ccccaagggg tttttcagta tgtggattaa gcttgagagg
     5161 ggcaggattc actatcatca acatcaacct tccgcaggaa aaaacaaaaa aatgaagacc
     5221 ttgtcgacat gtttcatagg cgttcttgct tttaccggaa ttattacaaa tgcggccgcg
     5281 gctggtgtca ttaccagcgt atcgggcgct ggcgggggac tgggatcatt tggcgttctc
     5341 aaccatgcga tcgtttcgaa gcaactgaat ctgatcaaga ccttcgatca tattgatccg
     5401 atcgatctga ctttcacggt gattcatggc caaggggcag gcgggtctta ctcggtaacc
     5461 gagacgatta ccaatcacac aggattacct tggggcgact tccatttcgt catttccgaa
     5521 ccggcggagg gaagccaggg aatggttttt accaatttcc aaagtgccag cctagcgggg
     5581 ttcacgttgg actccccgcc ttcaagtggc ccgcgcgagc ttaacttcac aggtaatctc
     5641 gcgagtggcg cctcagccac ggcaacgttc agcctgaatc tgcctgatcc aggagcgggc
     5701 aaaaccttga ccttcacctt gacgcaaaaa ccgagcctcg aaacgagtgt aagcccggtt
     5761 cccgaacccg aaacttatgg catgcttatg gccgggctcg gcattatttg cctggtagta
     5821 aaacgccgga gcagcaaaca gagcgtttaa tagtttctcc aggatgactg ttcttccccc
     5881 gcactggcag tcatccttgt cattctgcct tttcttcttc gcattccatc gttcatctgc
     5941 cggaaacccc ggttcaattt tcctgccctg gcccggcgtc ctggcaggca actcggccgg
     6001 tgactccagc gttaggatgc cgctgaataa gtgcggtatt tagtacctta gtccaatatt
     6061 catttccttc cagttacgcg aatatattcg tcagcaaggg gcctgcggcg acaatatagc
     6121 caaagagcaa tgatgattaa aatcgttaag ggaattgatg acaggatcaa acattccagg
     6181 caggaacaaa cttactgcgc gcgtcttggc cggctttgga atgctggcgg cttccctgtc
     6241 ggcacatgcg ctggtaatcg atatcaacag ctacgtcacg ggaagcgggg cgacgttaaa
     6301 tacgacggtg gccaccctta cgctgacgca aaacggctca aatgtggact tcagtttcaa
     6361 taatctcgta aacaatcttt cgagcggtgt gggggacgac gcgtttatct ccgaactatt
     6421 attttcttac gaccgcatgc cctcactcac gactgcttcg ttttcaaact tcggcgggag
     6481 ccagccaata accgcgtctg cctttgccat tagtcctcct gggaaggacg ctgggtacga
     6541 tttctatctg gccctgagct acccgaccag cacgggaaac cgatttaccc acggggagtt
     6601 ttccacttgg acaatcagca atgttgttat cgctgacttt cttcagccgg tatcgggcag
     6661 cggtccggct tcacttgcaa tggcgcatat ccagcaggtc ggcgctggac caggcggggt
     6721 cggttccgtg aaatatgccg gaagtgacaa ctccgatcct ccgcaactcg cagaaaatcc
     6781 ggtccccgag cctggatccc ttgcattgct actggccggg ttagttggct tcggtgctat
     6841 ttaccgacgc ccgtcgcgca caacgctata aaccggtgcc taccgaccag cccatgcgtt
     6901 cgagaaaacc gcggaacgca tcgcttgcga tgatgaatgc gataagatag gaagtcaggc
     6961 ttaacagaac gatagtccag ataaggcgcc aagggctcag cgtcgagtag cgcttccatt
     7021 gggatgaaaa gctggcgtct tcaggttcga gaggtttgtg ggattccagt tcgtaaagga
     7081 cgtttctgcg tgctttaagc aacgcggcag ttatgcccgt ccggtacgta cgatcgataa
     7141 aaccggcctg cttggcgccc cctgcggtcc cggcctcagg ctcggagact tcgggggtcg
     7201 cttccttgta aaagtcctgc attcggtcaa cataccggtc atcggccagc aactccccca
     7261 caatcgcttc gatatgacgg atatcctcgt tgtccaccgg agcagtcttc gccacggatt
     7321 gttcgccgga tttgcccttg agcgacttgt cgatctcctg aataaactcg ttttctttcc
     7381 gggaaatcag cttcagccac accgcctccg cagcctgggg agaaggacgt ctcaaattgt
     7441 tttcgctatc cttggcgttt tcaacaagct gagcgagttc gatctttgcc gcccgttcac
     7501 aatccgcgtc tatcgcctca agcctggagc cgagcatatt ccctcatcct gaatccctgt
     7561 agcgggcacc atataccacc tctagtttag cgtaaatcgc gggggaaagc ggcgctcgcg
     7621 cggccgtagt gtcacgtcgg gaaaacggga cgccgaggga aagccgagcg ggctgaaatc
     7681 gcgccgatgg accagaccgt gactgccccg aaagcgtgtg gactagatca taactgcccc
     7741 gaaagcgtct cgatcctcta ttgcggccgt ggcccggcga ataagttcat aattgttctc
     7801 aggtttcatc attcctccta ctgcaagcgt gatcaccatg gcttcgaagg gatagataag
     7861 gtgagcacgg tatctttgca tgaggttgtc cggttcgatg cctggaaccc cgtggcgttg
     7921 aagcgctgct gcataagcag aaacgaggct tgcttcatgg attctgcggg tctcggggtt
     7981 gagggaagtg gcaagaaagt aggcgacgtc tccgatccct tcaccgaaac gtacgagctg
     8041 ccaatcgaga aagccaggcc ggtatttgtg ccagaataaa ttcccgggat ggcaatcgtg
     8101 gtggacaagc gtctggggtg cgcttgacag aaaccgcata atggtcgacc gccgacgtgc
     8161 atagtggact gccagcgcgt gtagcgcaga aggtacggct tgtcctgcgc ggcgcagtcc
     8221 ccgcttcatg agtggaactg caagcgtagc gctcaggcca tcttcgaggc ggcgtacggg
     8281 tccggcaagc cagccaaatg tttgcaagag ttcagcttta ccccagaaat gggcgtgaaa
     8341 ccgggcaagt tcttccacga caagagcggc ttgtcctggc gtaagggtgt cacccgggct
     8401 gcccggagtt gccccgcatt cagtcacgtc tctcaaaacg agtaccgcgc ctctgccgcg
     8461 ttcgctctgc gccgcaagga aatcgggcac cgcggtgggt acgatctggg cggcttcgcg
     8521 gtaaaagcgt acttctgtat gcagcagcct tggtagagcg gtaatgatcc gcgcccgcca
     8581 tgccagcgat ggaagtttta caaaccatcg ccgcggcata tcattcgggc cgtcatgctc
     8641 taccctcaga cggactctgg tcgtggtgcc gatatcaacg gaaacaatgt gcacagcaga
     8701 tattcttatg ccggaatatc tgctatttac gacccgctgt gcccattcaa cggttaaatc
     8761 attggggcgt cgtacaagaa catcgtattt catcaacgtc atttggtaat ttaatcagtc
     8821 aataatccct gcacttcacg tgaaactttc ttgaaaccat tacatcagca taggaggccg
     8881 ggatggcagt gccaaaaaaa gggagccctg aggctccctt tgatatagcg gtcggaaggt
     8941 ctgcaataat ccccttattg taatttccat tccggatggt agtgggtttc cttgtaccag
     9001 tctttccaga tgaagtcggg atagatgatg ggcttggtgg gaatgacgaa cgttaccaga
     9061 tccacaactc ccgtcagcgt acgcccgagg ccttgcacaa tcccgacaat aaatccgaca
     9121 ggaacgccat aagcaggccc tttctctctg ctctctatca tcatgttctt gggaacttcc
     9181 cctatacccg ttaccgtgtt ggctatgccg tgcgccagtt tttcacccac gtgctggggg
     9241 taactgccct cggccatcga agcctgaggg gaaaagaata gcaatgcagc aacaaccagc
     9301 agcggcttca acatgttttt catcaatgcc tccctatagt taattaacaa taaaccgggt
     9361 taatactagc ataccgccga gacaagtgtc gcgctacact tcgaatattg gctccccgaa
     9421 caatatttga ttgcagcatc agaaaacacg gttgaatcgt atcccaaatt catcgaggtt
     9481 aaaacgccct tagcgtctac acagagcaat cattccggac tgttgcgtca ccgcgtctgg
     9541 atttcaaatt ataacggtag ggtagcaagt cgcaatatcc ctcccaacaa tatccgcctc
     9601 aacaatctct tgttacagta ataaacggag gcctcaataa acggcccgac gcagataatc
     9661 cgcgccgggc cgtccccctt gtagagggcg gttttccctc tagctgatgt caaagcacaa
     9721 cctatgccag aattcgatat tagctttagt gttattaaat acaaactctt atcaatactt
     9781 ctgaattttc ggcaacttat cgctagcaat aactgtaaag aattccgtca tttttgctct
     9841 ttcaaaccca taattctgca tgaatgcgag atatcgacaa caaggaacaa ggaacaagcg
     9901 acgaggttcc gtgctatcac acattcaatg atagagaggc aaatggggag gccaggaatc
     9961 gccccgcttt cgagcttcga tgcgcttcag gaaaacgccc atcacttccc tgtaaagggc
    10021 atccttaagt acggcatctt cgttgcccgc gtccacattg ggattgtcgt tgatctcaat
    10081 cacatagaag cggttgccga cctgcttgag gtcgaccccg taaaatccgt caccgataag
    10141 atttgcggat ctcagcgcaa ccgcgatcag ttgctcggga gcctggtcga gcgcaaccgc
    10201 ctcggtagcg ccttcgctca gtttgtgttg ctgggcgtcg cgcttgatga cttgccagtg
    10261 tcccgtagga aaacgatagc gcgcgacgaa cagaggtctc ctgtccaata ttccaacccg
    10321 ccagtcaaac tcggtcggta gccattcctg ggcaatgatg agcgctgatt tgccgagcag
    10381 ctcgggtatc ttggcgttga gctcttcttc tgttttaacc tttgctaccc cgcgggagaa
    10441 cgaactgtct ggttgcttca gtatgcaggg caggccaagc tccgggataa tccaggaaat
    10501 gttgccgcga tgcacaagca gggtttttgg cgtgcggatg ttatgctgcg aaaggagttc
    10561 cgccagatac accttattgt tgcatttgag gatggaatca ggatcatcga tgactataag
    10621 cctttccgcg agggcccggc gcgagaatcg gtaggtgtag tggttgacaa aggttgtatc
    10681 gcggatgaac aaagcatcga attgggggag acgccctgcg tcggcgcccg tgatgaactc
    10741 cacacgcatg cgcagatctt cagccgcctt cctgaatttc tggagggcct tggaattgga
    10801 aggaggctca gggttatcgg gattcaggag aatcgcaagg tcaaaccttg gaggtcgttt
    10861 ccttgccgaa tgatttcttc cggtgaagta acccaccgcc gcgcgtacag cgaattcgcg
    10921 ttgctccggt ggaatatcgc ttaccgtcgt cgcgcaaacg gagcgaagaa tccactgatc
    10981 atcatgacgc tcgaaatgag ccgatagcaa tggcgcttgc aagcgatcga aaagctgctg
    11041 gctgagtgaa tcgtatcgtt tgtcgatgct ttggccgaag taaatgccga gtatgaaatt
    11101 gttcatatct tgcggagcga gcaggcgctg gatcaagggg cccaagtcct cggtgagata
    11161 acgaaccagg ttctgcgact gcaaatcctc catggtcccg actttcggga gaggcttatg
    11221 cccgcgtgcc tccgcgagca gcgaaacgta gtatcccgag ctctggtact ggtacgattt
    11281 gcaaagattg aaaatcttga ctgacctgct tgccgcccgg cctcgcgccc gggaggcacc
    11341 atctgcatga aggggatcgg tgagataggc ctgggcggct actatgcgca catctggaat
    11401 atccagcggc caatcagcgg gctcatcgac gacaataagt atgttcatct gttatccgca
    11461 cccaccctcc attacgtcat ccgatgtacg ggtgacttca tcatttagcg ccaggctgcc
    11521 catcactttt ttgaggggag cctttcttgc tctcgcccgg cggcgtgcca gcactgcgaa
    11581 cgtttacgac cttgctcaac atatcgaacc agaagggggc acccagcgaa accgcaaata
    11641 tggtaatgag caacccgaac agtttataga gtacccagcc ggcaaatggc agcgatattt
    11701 caggtgagcg aaagtatccg ggatcctgag ggtcgagtga ccatccgatc ggcagttgaa
    11761 ggcttcgcgc tttctcgatg acaagctgta tacgggcatc ttctgcgagt ggaagtggct
    11821 cagaggagcc gggattgcta gtccccgggc ttgacacggc cttcgccgcc tcctgtgcgg
    11881 cggcgaccaa cgatgcgcgc aaggcgttgt cttgtgacag acgttgaacc agcatcacgg
    11941 tgtcggcatt agataccagc acgacaagcg ttgccagcac gagcaggaca tgctgcgtcc
    12001 atcgcttata ccagccgctg acccggttca tcgcatcgtg gaaccataac tcgatgttcc
    12061 tcctgaaggc ttccgcctgg tgttcgacag cgctgacctc gcggcgtgtt ttatccacga
    12121 ggacaaggag cgattcccgg gtatggccct cgggaaggcg catcagccca tcctgtatgt
    12181 ttccgagggg atccgggtat tgtgccgcaa tggtacggcc gaactccagt gctttcctca
    12241 aggtggcgcc agcgttcagg gccagtgcca tttccttagg gtttttggca gatgcgatag
    12301 ctgctcgccg cgcctccagc atctgcaatg cagcggtgac cccgcccaag ctcactttcg
    12361 cgtcaacagc gagcttagca agctccaggt ttcctggagc ctcgttcgag ttttttaagg
    12421 ccgcctcgta tgaaatcctt gccttgtcag caagagcatc cagggcggct cgtgcctgct
    12481 cctgtttatt cctggccgct gcaagggctt ctgcaagctg cggatttccc gagccagcag
    12541 ctgcggcctt ccgggcttcc tcataagcat catccgcgct ttcggcctgc gccaacaaat
    12601 cgccgtactc ttttgcgata gcgccccgta ttccgagaat atccagtaaa gccagggaaa
    12661 aattttccgg cgacatgtag gaaggggggc gggtgggctt ggccgggtta gaggcatact
    12721 gggaaatgcc cccgatcagc ccgtgattat agacttgctg ggccaggccc gtgaaggtgg
    12781 gatcgttgag gagatttttg accccttcga agaggttcct tccacgcttt tgaagtagcg
    12841 aggcgatcgc ctcattcagc gccgagcata tcaggctgag aagcagatag acaaaagtga
    12901 cgccaatcgc tacttccaat atactggaac cgaacattcc tcccccgttc aattagacaa
    12961 tgtatagctc agtctagtca gtatggttat cggtgacaag tttactctca tacacatcag
    13021 acatttcatg agcgagccgg acagcaacat tctgtcgccg tagaaaccat gagaagaatc
    13081 agcgttgcgc tgctgctgtt cgcggccgcc gggtttgcca gtgttgccag ttctgccaac
    13141 attcccggtt tcgctggcgc ctcggtagat gtcccggaca cgatagcgga acgggtaaaa
    13201 ccgtgcgtca tctgtcataa gcaaggtgat cagacggatg tcaacggcta ttacccgcgg
    13261 attgcgggaa agcccgatgg ctacctctat aatcagttgc gcaattttcg cgacggcagg
    13321 cgctattacc ggccgatggc tttactgctc gaaaacctgt cggatgaata tctgatggag
    13381 atggcccgct atttttcact gctcaagcaa ccttaccctc cgcccgagcg cgtggttttg
    13441 cccccggagg agatcaagct tgtgcgcaag ctgatcgagg agggcgatcc gtcgcgaagg
    13501 attccccctt gcagggaatg ccacggaaaa gaattgatgg gtacgaagcc atttatcccg
    13561 ggtttgctgg gattgccgcg ctcttacatg atcgcccagt ttggcgcatg gcgcagcggc
    13621 ggtctcatgc gcaggcaatc ggcaaattgc atgtcggaga tcgcgaggca gctcacccca
    13681 caggagagca atgccattgc tgcatggctt gccgcccagc caattcctca aaatgcggag
    13741 ccaggtacgg gactttccga cgaaatggcc aggcgttgcg gtattgatgg gcaacgggaa
    13801 gcagttcgat gaaagcgggg cggagagcgt ttgtaccctt ttttctggca acactcggct
    13861 tgc
//
LOCUS       DASSHJ010000240         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_154411, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000240 DASSHJ010000000
VERSION     DASSHJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154411"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFI43_09330"
     CDS             complement(<1..260)
                     /locus_tag="VFI43_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062366.1"
                     /translation="MLSPFADIAFAGAVDRKLPASPLTPRNQEAHGLQPVVPLKLVDD
                     MGRPIRLARPAGRIVSLSPSITEMVFEAGAGAKLVGIARHSD"
     gene            complement(409..1533)
                     /locus_tag="VFI43_09335"
     CDS             complement(409..1533)
                     /locus_tag="VFI43_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7062367.1"
                     /translation="MILVLNPDTRSDSAEYKQLMAHLANLPGIAARVHTEVGSEQTLT
                     EVYLIGNTKALLIEEMQNLPCVEHAVRISEEYRVLGRHKNDHRPTHFEYNGVRFGQDT
                     LNVFAGLCAVDTLEHVELMMQALRDNAQVCTRMGAYKPRTSPYSFQGHGKACLPYVFE
                     LAGKYGIKVIAMEITHESHLEEICEALYRTGNATGVMLQIGTRNTQNFELLKIVGRQQ
                     EFPVLIKRGFGITLDESLNAAEYLASEGNRKVVFGLRGMKTNVGDPHRNFVDFSHVPV
                     VKRLTRMPVCIDPSHSVGMRAASPEGILDIMHATAQGVIAGANMVLVDFHPVPGKALV
                     DGPQALLIRELPLFLEDVRIARSAYEQRAALVERYEERAA"
     gene            1736..2728
                     /gene="miaA"
                     /locus_tag="VFI43_09340"
     CDS             1736..2728
                     /gene="miaA"
                     /locus_tag="VFI43_09340"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7062368.1"
                     /translation="MMAQPSPSEIPPPHLRPPAILLMGPTASGKSGVALELASRLPVE
                     IISVDSAQVYRHMDIGTAKPDTPTREDVPHHLIDLVEPDEHYSAAQFVLDALAAMQDI
                     TSRGRIPLLAGGTMLYFHALLEGLSDLPAGDSNLRREIEDRAGKIGWSGMHRELLRID
                     PTSAARIKPTDSQRIQRALEICYLAGKPLSEILEAPRARELPYTFVRIALVPSDRTIL
                     HRRIARRFDKMLESGLIEEVRSIRSRFELNDSSTSMRCVGYRQVGMYLNGMIGAVEMR
                     EKAAAATRQLAKRQLTWLRAMKGLKNFDCLNETVTEQIYAYITADLRVKAEPFR"
     gene            2802..3164
                     /locus_tag="VFI43_09345"
     CDS             2802..3164
                     /locus_tag="VFI43_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7062369.1"
                     /translation="MNEKHGSIIFVAVLAGALCLGGCTRPEDKVHESWKTPEPKAQVD
                     DATLADMLKSALRADPETRNLDVRVETHNGEIMLSGTVNNQAQMDRAIMLAWMAEGVK
                     KVDNRLNVAGGGIAQAAK"
     gene            3248..>3458
                     /locus_tag="VFI43_09350"
     CDS             3248..>3458
                     /locus_tag="VFI43_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062370.1"
                     /translation="MISYLFLAVVLVLGVVLLVPRVRRILISNHLLKVFRKLLPQVSQ
                     TEQEALDAGTVWWEGDLFSGAPNWNK"
BASE COUNT          787 a          952 c          979 g          740 t
ORIGIN      
        1 tagtcactgt gacgggcaat gcctaccagc ttggctcccg ctccggcttc gaataccatc
       61 tcggtaatac tgggagacag tgaaacgata cgccccgctg gccgagccag gcggatggga
      121 cgccccatgt cgtcaacgag cttcagcggc acgaccggct gcaagccatg cgcttcttga
      181 tttcggggag tcaaaggcga agccgggagt ttgcggtcca cggcgccggc aaaggcaata
      241 tccgcgaaag gcgataacat caatacggac agcagttcaa aagcgcggcg acgcagttgg
      301 cgcctacctt taacagggat acaagctcca gggcaggtat cggaaacttt aaaagttaat
      361 ttaatagtta aagaaatcgc ggcgaacccg atgaagattg tgacccgatc aggccgcccg
      421 ctcctcgtaa cgctcgacca gcgctgcccg ttgctcgtaa gcgctccggg cgatccgaac
      481 gtcttccaga aaaagcggga gttccctgat cagcagggct tgcggaccat caaccaacgc
      541 tttgccgggc actggatgga aatccacaag caccatgttg gctcccgcaa tgacaccttg
      601 ggccgtggcg tgcatgatat ccagaatgcc ctccggcgaa gcagcccgca tcccgacgga
      661 atgggatggg tcgatgcaga cgggcatgcg agtcagacgc ttgaccaccg gcacgtgaga
      721 aaaatcgaca aaattgcggt gcgggtctcc cacgttggtc ttcattccac gcaatccaaa
      781 gacaaccttc cggttcccct cggaggccag atactctgca gcgttcaggg attcgtccag
      841 cgtaatgccg aagccgcgct tgatcaagac agggaactcc tgctggcggc cgacaatttt
      901 cagaagttcg aagttttgcg tattgcgcgt gccgatctgc agcatgacgc cagtggcatt
      961 accggtacgg taaagggcct cgcagatctc ctccaggtgc gactcatggg tgatttccat
     1021 cgcaatcacc ttgatcccgt atttccccgc cagttcgaac acatagggca agcacgcctt
     1081 gccatgaccc tggaatgaat acgggctggt gcggggcttg tacgcaccca tgcgggtgca
     1141 cacctgggca ttgtcccgta aagcctgcat catcaactcg acatgctcca aggtgtctac
     1201 cgcgcaaagc ccggcaaaca cgttcagcgt atcctggccg aagcgaaccc cgttatattc
     1261 gaaatgggta ggtctgtggt cgttcttgtg ccgtccgaga actcggtatt cctcggaaat
     1321 cctgacggcg tgctcgacac acgggagatt ttgcatttcc tcgatcagca acgctttcgt
     1381 attgccgatc aaatacacct ccgtaagtgt ctgctcgctc cccacctcgg tgtgcacacg
     1441 ggcggcaatt cccggcaggt tggcgaggtg cgccatcagt tgcttgtatt ccgcgctatc
     1501 tgaacgtgta tctggattca ggacaagaat catgtggtgc tgtcggactt ggccggcgaa
     1561 ctggaccttc gcgtttgcct atctgcgcct gtctgtggaa aacttcaatt ctaaccgaaa
     1621 aaaccgatgc ttgccatccg ggtgaatatg gcgtttaatc gcctccggca ctactcgaac
     1681 aataaaacca tccgccggcc gcgaacgaaa atgctccttt cgccctgatt gctgcatgat
     1741 ggcgcaacct tccccatcag agataccacc accccacctc cgtcctcctg ccattttact
     1801 catgggccct acggcgagcg gaaaaagcgg tgttgctctt gaactggcca gccgcctgcc
     1861 ggtggagatc atcagcgtcg attcggcgca agtgtatcgc catatggaca tcggtacggc
     1921 caaaccggat actccaacca gagaagacgt tcctcaccat cttatcgatc tcgtcgagcc
     1981 tgacgagcat tactcggctg cgcagtttgt gctcgacgcg cttgcggcaa tgcaagatat
     2041 aaccagccgg ggaaggatac cgttgcttgc gggtggcacg atgctctatt tccacgctct
     2101 gctggagggg ctctcggatc tgcccgcggg ggacagcaac ctgcgccggg aaatagaaga
     2161 ccgggccggg aagatcggct ggtccggaat gcaccgggag ttgctgcgta tcgatccgac
     2221 gagcgctgcg cggatcaaac cgaccgatag ccagcggatt caacgggcgc tggaaatttg
     2281 ctaccttgca gggaaaccct tgtcggaaat tctggaagcg ccccgcgcgc gcgaacttcc
     2341 atataccttt gtcaggatag cgcttgtccc ctccgatcga acgatattac accggcgtat
     2401 tgcccggcgg ttcgataaaa tgctcgagtc ggggttgatc gaagaggtcc gatcgatccg
     2461 aagcaggttt gaattgaacg actccagcac gtcgatgcgt tgtgtcggtt accgccaggt
     2521 agggatgtat ctgaacggaa tgatcggtgc ggtggaaatg cgggaaaagg ctgcggcggc
     2581 tacccggcag cttgccaagc ggcagttgac ctggttgcgt gcaatgaaag ggctgaagaa
     2641 ttttgactgc ctgaacgaaa ccgtgaccga gcagatatat gcatatataa cagcagacct
     2701 gagggtgaag gctgagccgt tccggtagct gacgagttat aattcacagt cgatgctcaa
     2761 atcgtttgtc attttttgat caactttgat tgggaaatat catgaatgaa aagcatggat
     2821 caattatttt cgtagcggta ctggcaggag cgctgtgcct cggtggctgc acgcgccctg
     2881 aggacaaggt gcatgaatcc tggaaaacac ctgagccaaa ggcgcaggtt gatgacgcca
     2941 cccttgctga catgttgaaa tcagcgctgc gggccgatcc ggagaccagg aatctggacg
     3001 tcagggtcga gacccataat ggcgagatca tgctgagcgg aacggtcaac aatcaggctc
     3061 agatggatcg ggcaatcatg ctggcctgga tggcggaagg cgtcaagaag gtggacaaca
     3121 ggttgaatgt tgctggcgga ggcattgcac aagcagccaa atgagttttt cctctgactg
     3181 tccaacgcat ccggaaaggg ctgctaaccg ggaaaaactc tccaggcgct gacacaggat
     3241 aattatcttg atttcttatt tatttctcgc ggttgtcctg gttctaggcg ttgtcctttt
     3301 agtgcctcgg gtgcgtcgca ttctcatctc caaccatctg ctcaaggttt ttcgcaagct
     3361 ccttccccag gtttcgcaaa ccgagcagga agcgctcgat gcaggcacgg tctggtggga
     3421 aggagatttg ttcagcgggg cgccaaactg gaacaagc
//
LOCUS       DASSHJ010000241         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_697513, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000241 DASSHJ010000000
VERSION     DASSHJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697513"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="VFI43_09355"
     CDS             complement(<1..102)
                     /locus_tag="VFI43_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7062371.1"
                     /translation="MLHLTLRQLKVFESVARNLSFSRAAEELYLTQPA"
     gene            284..1753
                     /locus_tag="VFI43_09360"
     CDS             284..1753
                     /locus_tag="VFI43_09360"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HET7062372.1"
                     /translation="MSQEIQGKERYKSGVIPYKKMGYWDADYVPKDTDIIALFRITPQ
                     EGVDHEEAAAAVAGESSTATWTVVWTDRLTACELYRAKAYRSDPVPNTGPGTKTEQQY
                     FAYIAYDLDLFEPGSIANLTASIIGNVFGFKAVKALRLEDMRIPVAYLKTFQGPATGI
                     VVERERLDKFGRPLLGATTKPKLGLSGRNYGRVVYEGLKGGLDFMKDDENINSQPFMH
                     WRDRFLYCMEAVNKASAATGEVKGHYLNVTAGTMEDMYERAEFAKSLGSVIVMIDLVV
                     GYTAIQSMAKWARKNDMILHLHRAGNSTYSRQKNHGMNFRVICKWMRMAGVDHIHAGT
                     VVGKLEGDPLMIKGFYDTLRDTHTPKNLELGLFFDQDWASLNKVMPVASGGIHAGQMH
                     QLLDYLGEDVILQFGGGTIGHPMGIQAGAVANRVALEAMVMARNEGRDYVKEGPKILE
                     AAAKWCTPLKQALDTWKDITFNYESTDTADFVPSETASV"
     gene            1777..2211
                     /locus_tag="VFI43_09365"
     CDS             1777..2211
                     /locus_tag="VFI43_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HET7062373.1"
                     /translation="MMTNFQNRITQGQFSFLPPLTDKQISAQIKYALKNNWAIGIEYT
                     DDPHPRNTYWEMFGNPMFDLKDPAGILTEINDCRKTYPNHYIRVTAFDATRGTESPKM
                     SYIVNRPKNEPGFRVNRQEADSRSIRYTIHSYAMDKPEGERY"
     gene            2393..3355
                     /gene="cbbX"
                     /locus_tag="VFI43_09370"
     CDS             2393..3355
                     /gene="cbbX"
                     /locus_tag="VFI43_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HET7062374.1"
                     /translation="MKKSSKDLVVVAADTSPIDLDAEFRASNIKEVLDKLDQELIGLK
                     PVKTRIRETAALLLVDRLRKKLDLTAGAPSLHMSFTGNPGTGKTTVALRMSEILHRLG
                     YVREGHLVSVTRDDLVGQYIGHTAPKTKEVIKKAMGGVLFIDEAYYLYKPENERDYGA
                     ESIEILLQVMENNRDDLVVILAGYKDRMEKFFRSNPGMRSRIAHHIDFPDYTPDELVA
                     IAKLMLAAQNYRFSAEAEKAFGEYIQLRMKMEHFANARSVRNALDRARLRQANRLFEG
                     AKKNLSRADLMTIEAEDIRASRVFKEGAVDLDGDEGANERIVAG"
     gene            3566..>4015
                     /locus_tag="VFI43_09375"
     CDS             3566..>4015
                     /locus_tag="VFI43_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET7062375.1"
                     /translation="MRPTRLEKPSHNRNDWKTIRALMPYLWEFKGRVTAALVLLILAK
                     LANVTVPLVLKEIVDALDQPRALLALPVLLLAAYGALRLFSTLFGELRDAVFAKVTQR
                     AIRRVAIKVFGHLHALSLRFHLERQTGGVSRDIERGSRGISFLLNFML"
BASE COUNT          983 a         1055 c         1106 g          871 t
ORIGIN      
        1 cgcgggctgc gtgaggtaga gttcttcagc cgcgcgagag aagctcaggt tgcgggccac
       61 agactcgaaa actttcaatt ggcgcagggt caggtgcaac atggcgattg gaaagtaagc
      121 gtcgcattgg ccattgcaga gtaaaaaatt ctgcgggctg aaaggtaacg tacctataag
      181 gtaaatatta taataacaat taaaacatgt gactgttgtt tataataaat cgggttacaa
      241 tttgacctgt agtacttttt tctaccccta ccaggagact gacatgagcc aagaaataca
      301 gggcaaggag cgctataaat ccggcgtaat tccctataaa aaaatgggat attgggatgc
      361 tgactacgta ccgaaggata cggacattat tgccttgttc cgtattaccc cgcaagaagg
      421 tgtggatcat gaggaagcgg cagctgctgt tgccggtgaa tcttccaccg caacctggac
      481 ggtggtatgg accgatcgcc tgactgcatg cgaactctat cgcgcgaagg cctatcgttc
      541 cgatccggtt cccaataccg gtccaggaac caaaacggag caacagtact ttgcctacat
      601 tgcttatgac ctggatttgt tcgagcccgg ctccatcgcc aacctgaccg cctcgatcat
      661 cggcaacgtt tttggtttca aggccgtaaa agcgttgcgc ctggaagaca tgcgcatccc
      721 cgtcgcttac ctgaagactt tccagggtcc tgcgaccggt attgtagtgg agcgggaacg
      781 tctcgacaaa ttcggccgtc cgttgctcgg tgcaaccacc aaacccaagc tcggcctttc
      841 cgggcgcaac tacgggcgcg tggtgtacga gggactcaag ggtggtctgg acttcatgaa
      901 ggacgacgag aacatcaatt cccagccctt catgcactgg cgcgaccgct tcctgtactg
      961 tatggaagcc gtcaacaagg cttccgccgc aacgggtgaa gtcaagggtc actacctgaa
     1021 cgtcacggca gggacgatgg aagatatgta tgagcgggct gagttcgcca aatcgctcgg
     1081 atctgtcatc gtcatgatcg acttggtggt aggttacacc gccatccagt cgatggccaa
     1141 gtgggcacgc aagaacgaca tgattctgca cctgcaccgt gccggcaact ccacctattc
     1201 tcgtcagaaa aaccacggca tgaacttccg cgtgatctgc aaatggatgc ggatggcggg
     1261 tgtggatcat attcatgcgg ggacagtcgt cggcaagctc gagggcgatc cgctcatgat
     1321 caagggcttc tacgatacgc tgcgtgacac ccacactccg aaaaaccttg agctcggcct
     1381 gttcttcgac caagactggg cttcgctgaa caaggtgatg ccggtcgcct ccggcggcat
     1441 ccacgctggg cagatgcacc agctgctgga ttacctcggc gaagacgtga tactgcaatt
     1501 tggcggtggc acgattgggc acccgatggg tatccaggca ggcgcggttg ccaaccgtgt
     1561 tgcgcttgaa gcaatggtca tggcgcgcaa tgaaggccgc gactacgtga aagaaggtcc
     1621 caagatcctg gaagctgctg cgaagtggtg cacgccgctc aagcaagccc tggatacgtg
     1681 gaaggatatc acgttcaatt acgaatccac tgacactgcc gacttcgtcc ccagcgagac
     1741 ggccagcgtt taattgcact taggagaaat cacgtcatga tgacaaattt tcaaaaccgt
     1801 atcacgcagg ggcagttctc ttttctgccc ccgttgacgg acaagcagat ctcggcgcaa
     1861 atcaaatacg cgctgaagaa taactgggca attggtatcg aatacacgga tgacccgcat
     1921 ccacgcaaca cctactggga aatgttcggt aacccgatgt ttgacctcaa ggatcctgcc
     1981 ggcattctga cggagatcaa tgactgccgc aaaacgtatc cgaatcatta cattcgggtg
     2041 actgcgtttg acgccacgcg tggaacggaa agcccgaaga tgtcgtacat cgtcaaccgg
     2101 ccgaagaatg aaccgggctt cagggtgaat cggcaggaag cggatagccg tagcattcgt
     2161 tataccatcc attcttatgc aatggataaa ccggaaggtg agcggtatta agcggtgaaa
     2221 cccatcccct tcccagcgaa ggggatggca aacaaatgct gattacgtta tgagcggagc
     2281 aaggaaagga gcgcctgtta atctggcgtt ccctttctga accgcggtac aactctgttg
     2341 tcaatggagt tgaaatatgc agagcgcaca tattgtttat ggtgaatcgg atatgaaaaa
     2401 aagctcaaag gatctcgttg tcgttgccgc ggataccagc ccgatcgatc tggatgcaga
     2461 gtttcgcgca tcgaatatca aggaagtgct ggacaagctt gatcaggaat tgatcggctt
     2521 aaagccggtt aaaaccagaa tccgggaaac cgctgccttg cttctcgtcg acaggctgcg
     2581 taaaaaactg gatctgactg ccggggcacc cagtctgcac atgagcttta ccggcaatcc
     2641 gggcaccgga aaaaccacgg tggcgctgcg catgtcggag atcctgcaca gattgggtta
     2701 tgtgcgcgag ggacatcttg tttccgttac gcgcgacgat ctggtgggcc aatatatcgg
     2761 ccatacagcc cccaagacca aggaagtcat aaagaaagcg atggggggag tgctgttcat
     2821 cgatgaggcc tattatctgt acaagcccga aaacgaacgt gattacggcg cggagtcgat
     2881 cgagattctg ttgcaggtga tggaaaacaa ccgggacgat ctggttgtaa tccttgccgg
     2941 ctacaaggat cgcatggaga aattcttccg gagcaatcct ggcatgcgtt cgcgtattgc
     3001 acaccatatc gacttccccg actacacccc ggacgagctc gtggcgattg ccaagctcat
     3061 gctcgccgca cagaattacc gcttcagcgc cgaagccgaa aaagctttcg gcgagtacat
     3121 ccaactaagg atgaaaatgg agcattttgc caatgcacgc tcggttcgca acgcacttga
     3181 ccgtgcccgg ctccggcagg ccaatcgctt gttcgagggg gcgaagaaga acttgtcccg
     3241 agccgatctg atgaccatcg aagccgagga cattcgcgcc agccgcgtat tcaaggaagg
     3301 cgccgttgat ctggatggtg acgaaggggc gaacgagcga attgtggcgg gctgatccat
     3361 cattcttgcc tctaacagta taaaagactc agaacccggc agaagaccca tagctgaagt
     3421 taatccatca aatgaagggc gtccatccca ggacgccctt tttgtttgag ggacaaagac
     3481 agtttgctgt aattcaatga agaagagtgc ttcactgttg cacgcttctc ccgatcatat
     3541 tccaaagtcg ctcggcgact aacccatgcg ccccactcgt ctggaaaaac cgtcgcataa
     3601 ccggaacgac tggaaaacaa ttcgggcgct gatgccttac ctgtgggaat tcaaaggcag
     3661 agttacagct gcgctggtgc ttctgatact ggccaagctc gccaacgtga cggttccgct
     3721 ggtactgaag gagattgtcg acgctctcga tcagccacgc gcgctccttg ccttgccggt
     3781 tctcctactg gccgcctacg gggcgctgcg cctgttcagt accctatttg gagagcttcg
     3841 cgacgcggtg tttgcgaagg ttacccagcg cgccatccgg cgcgttgcca tcaaggtttt
     3901 cggccacctc cacgcgctgt cgttacgctt ccacctggaa cggcaaacgg ggggagtttc
     3961 tcgcgatatc gagcgtggtt cgcgaggcat ttcctttctg cttaatttca tgttg
//
LOCUS       DASSHJ010000242        12426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_262515, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000242 DASSHJ010000000
VERSION     DASSHJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262515"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..3621
                     /gene="smc"
                     /locus_tag="VFI43_09380"
     CDS             67..3621
                     /gene="smc"
                     /locus_tag="VFI43_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET7062376.1"
                     /translation="MRLSHIKLSGFKSFVDPTEIPTPGDLVGIVGPNGCGKSNVIDAV
                     RWVLGESRASALRGESMQDVIFNGSGNRKAVGRASVELVFDNSLGKAAGQWSTYAQIS
                     IKRVLRRNGESSYHINNIHVRRRDVADIFLGTGVGGRGYAIIEQGMISRIIDAKPEEL
                     RMFLEETAGISRYRERRRETEQRLGDTSENLLRVSDICQELEKQLARLEEQAHVATRF
                     RDVQGRLRRAQELLSYARRQEAAAQRESAEKELESLQVLLEAETARLHAAETRLEDKR
                     AQHYASGDALHQAQGALYSTNAEIAHLEQQIQHLRENSQRVAAQISSVNNRIDYREGQ
                     AREAEGTLSQLHTELEQARQSHEQAKSKATLENEKLPGVEAAFRACQQKSAQTEHALL
                     RAEQAGRLEENHRAYAGKTIQQLELRRTRLLTELSGLPAPEMEELSQLHFEAEISASE
                     LNQKQGALAEAESLLSATGGAVRSCVDKVRVAHQRIIQAEARLHALERLQHRLEGSDK
                     LSAWLAKHQLDALPRLWQSIQIESGWEDALEAVLRERLNSAQLERLEFASAWVRDPPP
                     AKWTVYEPTGRQVPNEQTGCPSRSGIGWRTLQSYLSFDNTQIRRVMEEWLSGVFAVES
                     IEEGLARRTTLFPREMFATREGHIVTYHSLSYYSPDSQLHGVLGRQREIGQIRLELGT
                     LKNNLALEQSSLDAAEESKRELGSSVSRLRFDIAQLQQQSHDIQMQRLKLVQLAERAS
                     SRRGQINDELAEIEQQMTSEVSATCEAETRLAQLKAEIETLREMAKEEKGATQDAERF
                     LSVQRHALQNALREVQEALFREKTCHNKMAETENTINSIKEDLAEFRTQLQKLQAEQG
                     SFDETELNSRLHEWLTLRGQREHALAIARNVLEEAARLLRNIEQERLAAEQKLHPLRE
                     SISAARLKEQEARIIENQFDEQLKESSLSQEELAQALGKTRPSALQAEIVRLDGEIKA
                     LGAVNLGALQELESARVRKGYLDSQLKDLREASDTLKDAIRRMDAETRERLLETVEKV
                     NANLNEMFPTIFGGGQARLLLKGDEILDAGVQIIAQPPGKKNSSIQLLSGGEKALTAL
                     AFVFSLFQLNPAPFCLLDEVDAPLDDSNTVRFCNMVRKMARQTQFIFISHNKITMEMA
                     QQLIGVTMQEKGVSRVVAVEMEDAARLGSTPQRAGQAW"
     gene            3624..4787
                     /locus_tag="VFI43_09385"
     CDS             3624..4787
                     /locus_tag="VFI43_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HET7062377.1"
                     /translation="MSELQVSLIAIGFIVVMGVIFFNWLQQRRYRRHAEQAFSQKHED
                     VLLRTTAPLEEGRIEPQLGPAETSHEVEAEPKLNEPVEAAPETPEQPVTPAVSLERPS
                     APVEKPAPPLTAVFDGTVDYVVNISSEKKIENSGLGALLQRKFDFSKPVRWLGQTGSE
                     TAWEDITSDVNGKADYFALRGCLQLADRAGPVSEVHLSEFRDMAENFCAGINARARCP
                     DVHKAYAQALALDEFCSEVDVMIGITIISRDGGAVTGAKIRMVAEASGFKLGADGMFS
                     FRGENGSTLFSLGNLEPHPFLPDTLRTLTTRGVTFLLDVPRTSNGEMVFEDMVRVAEA
                     FADSVGGIMVDDNNIPLSAEGIEKIQQQLMAIESMMATRGIPAGSEVARRLFA"
     gene            4784..6838
                     /gene="ligA"
                     /locus_tag="VFI43_09390"
     CDS             4784..6838
                     /gene="ligA"
                     /locus_tag="VFI43_09390"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7062378.1"
                     /translation="MNPIPHHLADRAGELRKLIELHNYNYYALDAPTIPDAEYDTLFR
                     ELLDLEQQYPELATPDSPTQRVGLAALKQFSQIHHRTPMLSLINAFTAEDVEGFDRRV
                     RQLLSVDEVEYAVEPKFDGLAVSLCYENGVLTSGATRGDGYVGEDVTHNLRTIKSIPL
                     QLHADSHEPPRFLEVRGEVLMLRADFEKLNQHQREKNEREFINPRNAAAGSLRQLDPA
                     ITAGRRLTFFAHGVGACEGGKIPRDRHTHLLDYLASKRFPVPRERDTVRGFPALLEYH
                     GKISRMREALPYDIDGTVYKVNDLAQQEKLGFVSRAPRFAIAHKFPAQEAVTEVLAIE
                     VNVGRTGALTPVARLKPVFVGGVTVTNATLHNEDEVRRKDVMIGDSVIVRRAGDVIPE
                     IVAVQKEKRPQDAKFFVMPLHCPICGSKVVRLPDEAVSRCTGGLFCPAQRKQSILHFA
                     SRRAMNIKGLGEKLVDHLVDRGIVKTPADLYKLGSTDIAALERMADKSAGNIINAIEK
                     SKATTLPRFIYALGIRNVGETTARDLASHFGALDRLIAADIQSLQHVRDIGPVVAESV
                     ANFFAERHNLEVIDQLRESRVRWEESQSTEQAAPGGAAELLGRVAGRTFVLTGTLPHL
                     SREEARERIEAEGGKVVGSVSKKTDYVVAGDDPGSKYEKAVELGITILDEEGLLNLLQ
                     TS"
     gene            6864..7751
                     /gene="galU"
                     /locus_tag="VFI43_09395"
     CDS             6864..7751
                     /gene="galU"
                     /locus_tag="VFI43_09395"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET7062379.1"
                     /translation="MPKITKAVFPVAGMGTRFLPATKASPKEMMPVVDKPLIQYAAEE
                     AVAAGIRELIFITGRHKRAIEDHFDKAYEMEAELEARHKYDTLAILRSILPKDISCIY
                     IRQPEALGLGHAISLSRPAVGNEPFAVLLADDLLVGNTPVMQQLAEIYEQNNCSILAV
                     ENIEREETSNYGIVKCEPGMKRVHPVTGIVEKPRPEDAPSTLGVVGRYILTPGIFRHL
                     EKIRPGAGGEIQLTDGIASLLEEERVLAYEFRGIRYDCGSKLGYLKATVAMALEHPEV
                     AQPFAEYLATLPCCRPLAS"
     gene            7856..8443
                     /locus_tag="VFI43_09400"
     CDS             7856..8443
                     /locus_tag="VFI43_09400"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HET7062380.1"
                     /translation="MLTFEEANNIMSSAEPLFSAQEIAKTIRRMATEITVALANDYPV
                     VLSVMGGAVVFTGHLLPLLEFPLTFDYLHVSRYANTRHGGEIRWKMRPQEDIRGRVVL
                     VLDDILDEGVTLAAIRDWAVSRGASGFYSAVLVEKEIGRPKPIHADFTGITLPDRYVF
                     GFGMDINGAWRNLPAIYALKDKGFPKTEQPFSNQF"
     gene            8798..9544
                     /locus_tag="VFI43_09405"
     CDS             8798..9544
                     /locus_tag="VFI43_09405"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HET7062381.1"
                     /translation="MLAIIGGTGMAQLPCLEISHRQIIRTPYGEPSGPFTFGSINSNK
                     VVFLARHGYGHTIPPNEVNYRANLWALRSLDLMNVVAVASVGGIRADLAPGVLAVPDQ
                     IIDYTHGRHYTYYEGTDRPVTHIDFTEPYCQSMRNRLLKAALLAGEKVIDGGVYGATQ
                     GPRLETAAEINRMERDGVDMVGMTGMPEASLARELGLCYATVAVVANYAAGRKTSAHS
                     ISLEDAHSVLERAMVRVRDILERVVETDGD"
     gene            9534..10067
                     /gene="def"
                     /locus_tag="VFI43_09410"
     CDS             9534..10067
                     /gene="def"
                     /locus_tag="VFI43_09410"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7062382.1"
                     /translation="MAIRPVLKMGDPRLLEVAEKVKSFGTPALHNLIRDMRDIMESLD
                     GAGLAAPQIGVGLQVVIFGVKRNPRYPDAEEVPYTVLVNPVLTPLTDEMEPGWEGCLS
                     VPGLRGMVPRFARLRYEGSDQYGRRIERTVEGFHARVVQHECDHLQGILYPMRISDFR
                     TFGFTDVLFPAEAPMEE"
     gene            complement(10222..10803)
                     /locus_tag="VFI43_09415"
     CDS             complement(10222..10803)
                     /locus_tag="VFI43_09415"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7062383.1"
                     /translation="METLNMKMAEDEVPGEASGNIFLIGMMGAGKTTVGRLLAHFLGK
                     TFYDSDREIQKRTGVGIPLIFEIEGEAGFRKRETDMLAELAQMENIVLATGGGSVLNT
                     ENCRILKQRGIVIYLRAAVEDLWRRTRQDQNRPLLRTVDPRKKLTELYAQRDPLYRDA
                     AHIVVESGKRSARHLAQLLAQQLACSGLRSHNG"
     gene            complement(10880..11236)
                     /locus_tag="VFI43_09420"
     CDS             complement(10880..11236)
                     /locus_tag="VFI43_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062384.1"
                     /translation="MKSSLEEERMAILERMDATRKNYRRMLNIEDENEKEHLHRSVAD
                     TFPRSHTFKFITRHPYFTSLAVLGLIAAMPHGSLKKAAKGGAAITAGILRNELKSSLI
                     NRALPSVLHLMRSRRI"
     gene            complement(11233..11601)
                     /locus_tag="VFI43_09425"
     CDS             complement(11233..11601)
                     /locus_tag="VFI43_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7062385.1"
                     /translation="MFATLEKAQQLGAIALERLGDYVELFKVSAEIQGQSLIKRVIGA
                     VVAGLFAALSLMFLGAAVIVTFWDTPYRVMSAWGVAGFYALIAFIIYLSMPARNDSVS
                     ALDTLRNELQQDVKFMKDVV"
     gene            complement(11633..12046)
                     /locus_tag="VFI43_09430"
     CDS             complement(11633..12046)
                     /locus_tag="VFI43_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HET7062386.1"
                     /translation="MQTDTSANKTDDAQGAAENMAHHVGSAAQRVGEAAKSAGKHVGA
                     VASEELSNLRADLDDLIAKIPTLSDVDLEDAKEKLMQKIAETRESAKSIAGNAREQFQ
                     HGVECSKEYVKDRPLESVGYAAAAGFLLGLLMTRR"
BASE COUNT         2901 a         3221 c         3652 g         2652 t
ORIGIN      
        1 ctttcgagca cttcccggcc cgcatattat agctgttgca aactaaagcc ggttgcgaac
       61 aaaaaattgc gcctctccca cattaaactc tctggtttca aatccttcgt cgatccaacg
      121 gaaataccta ctcccggcga tcttgtcggc attgtagggc ctaatggctg cggcaaatcc
      181 aacgtcattg acgcggtacg ctgggtgctg ggcgaatcgc gggcctcggc gcttcgtggc
      241 gagtcaatgc aggatgtcat tttcaacggg tccggcaatc gcaaagcggt tggacgtgca
      301 agcgtcgaac tcgtctttga caatagcctg ggaaaggccg ccgggcaatg gtcgacctat
      361 gcccaaatct cgatcaagcg ggtgttgcgg aggaacggcg aatcgagcta tcacatcaac
      421 aacattcatg tacggcgcag ggatgtggct gatattttcc tcgggaccgg agtgggtggg
      481 cgcgggtatg ccattatcga gcagggaatg atctctcgca tcatcgacgc caagccggaa
      541 gagttgcgca tgttccttga agaaacagca gggatatcca gataccgtga acgccggcgg
      601 gagacggaac agcgtttggg ggacacttct gaaaaccttc tgcgcgtcag cgatatttgc
      661 caggaactgg agaagcagtt ggcgcgactc gaagagcagg cacacgttgc aaccaggttc
      721 agggatgtcc aaggccgctt gcgcagagcg caggaactct tgtcgtatgc gcgcaggcag
      781 gaagcggccg cgcagcggga atcggcggaa aaggaactcg aatccctgca ggtgctgctc
      841 gaagccgaga ccgcacggtt gcatgccgcg gaaacacggc tggaagacaa gcgggctcag
      901 cactatgcga gcggcgacgc gctccatcag gcgcagggag cgctttattc cacgaatgct
      961 gaaattgctc atctcgagca gcagattcag cacttgcgcg agaacagtca acgtgtagcc
     1021 gcacagatct cttccgtgaa taatcgcatc gattatcgcg aaggccaggc aagggaagcg
     1081 gaagggacat tgagccagtt gcatacggag ctcgaacagg cgcggcagtc tcatgagcag
     1141 gcgaagagca aggcgacgct tgaaaacgaa aaattgcccg gggtggaggc tgcttttcgg
     1201 gcttgccagc aaaaatcagc ccagacggag catgcgctct tgagggccga gcaggccggg
     1261 cgactggagg agaaccaccg ggcttatgcc ggaaagacca tccagcagct cgaattgcgc
     1321 cggaccaggc tcctgaccga gttgagtggg ttaccggcgc ccgaaatgga agagttatcg
     1381 cagttgcact tcgaggcaga gataagtgcg tccgagctca accagaagca gggggccctg
     1441 gcagaggctg aaagtttgct ctccgctacg gggggagcag tccggtcctg cgtggacaag
     1501 gtgcgagtag cgcatcagcg gatcattcag gctgaagcga gattgcatgc cctggaacgc
     1561 ttacagcacc ggctggaagg tagtgacaag ttgagtgcgt ggctggcgaa gcatcagctg
     1621 gatgcgttgc cgcgtttatg gcagagcata cagatcgaaa gtggttggga agacgcactt
     1681 gaagcagtgc ttcgcgagcg cctcaacagc gcccagctcg agcgactgga gttcgcttcc
     1741 gcgtgggtgc gtgatcctcc tcctgcgaag tggacagtat acgagcccac ggggcggcaa
     1801 gtgcccaatg aacaaaccgg atgtccatca cgctcgggga tcggttggag gacgttgcaa
     1861 tcctatctct cattcgataa cacgcagata agacgtgtaa tggaagaatg gctgagtggc
     1921 gttttcgcgg tagagagcat agaggaaggt ctcgcacgcc gaacgacgct tttccctaga
     1981 gaaatgtttg cgacgcgcga gggccatatt gtcacttatc acagcctcag ctattactct
     2041 cctgactcgc agctgcatgg cgtattaggg cgccagcgtg aaataggtca aatcaggctc
     2101 gaacttggaa cgttgaagaa taatctggcg ttggagcaat cttcgctgga tgcggccgaa
     2161 gagtcaaaac gggaactcgg atcgtcggta tctcgcttgc gttttgatat tgctcagctt
     2221 cagcagcaga gtcacgatat tcaaatgcag aggctcaagc ttgtacaact cgccgagcgt
     2281 gccagttcga gacgtggcca gattaacgac gagctggcgg aaatcgagca gcagatgaca
     2341 tcagaggttt cggcaacgtg tgaagcggaa acaagacttg cccaactcaa ggcggaaatc
     2401 gagactttgc gcgaaatggc aaaggaagag aaaggtgcga cccaggacgc tgaacgattc
     2461 ttgagcgtcc aacggcatgc gctccaaaac gcattgcgcg aggttcagga ggccctgttc
     2521 cgtgagaaaa catgccataa caaaatggcc gaaacagaga atacaatcaa ttcgattaag
     2581 gaggatctgg ccgaattcag gacacagttg caaaaattac aggctgaaca gggcagtttc
     2641 gatgaaacgg agctcaacag tcgcctgcac gaatggttga cattgcgggg acagcgggag
     2701 catgcgctgg cgattgctcg caatgtgctc gaggaagccg ctcgccttct gcgcaacatc
     2761 gagcaggagc gattggctgc cgagcaaaag cttcatcctc ttcgcgaatc catcagtgcg
     2821 gcacgtctca aggaacagga ggcgcgcatt atcgagaatc agtttgatga gcaactgaag
     2881 gaatcgagcc tctcccagga agagttggca caagctctag gtaaaacccg cccctcggcg
     2941 cttcaggccg agattgttcg cctggacggg gaaatcaagg cactgggggc ggtcaatctt
     3001 ggcgcgctcc aggaattgga gtcggcccgg gtccgcaagg gctacctcga ttcgcagctc
     3061 aaggatttgc gggaggcttc cgatacatta aaggatgcaa tacggcgcat ggatgcggaa
     3121 acccgcgagc gcttgctgga aaccgtcgaa aaggtgaacg ccaacctgaa tgaaatgttt
     3181 ccaaccatct tcggcggggg gcaggcgcgg ctgctgctga agggtgacga gatactggac
     3241 gcgggtgtac agatcattgc gcagcctccc gggaagaaga acagctcgat ccaattgtta
     3301 tcgggcggcg aaaaggcctt gactgcgctg gcattcgtgt tttcgctgtt ccaactcaac
     3361 cctgcgccat tctgccttct ggacgaggtg gatgcgccgt tggatgacag caacactgtg
     3421 cgcttctgca acatggtaag aaaaatggca cggcaaactc agttcatttt tatcagccac
     3481 aacaagatca cgatggaaat ggcgcagcaa ttgataggcg taaccatgca ggaaaaaggt
     3541 gtatcccgag tggtcgcagt agagatggag gatgccgcca ggctcggcag tacgccgcaa
     3601 agggccggac aggcatggtg aatatgagcg aacttcaggt cagcctgatc gcgattggct
     3661 ttatcgtggt aatgggcgta attttcttta actggctcca gcagcggcgt taccggaggc
     3721 atgcggaaca ggcctttagt cagaaacatg aagacgtgct gttgcgaaca accgcaccgc
     3781 tggaggaggg aaggatagag ccccagcttg ggccggcgga gacgtcgcac gaggttgagg
     3841 cggagccaaa attaaacgaa ccggttgagg ctgcgccgga aactccggag caaccggtca
     3901 ctcccgccgt gtccctcgag cggccatccg cccctgttga aaagccagct ccgccgttga
     3961 cagcagtctt tgacggaacg gtggattacg tggtgaatat cagttctgaa aaaaagattg
     4021 aaaattccgg cctaggggcg ttgctgcagc gtaaattcga tttcagcaag ccagtacgat
     4081 ggctagggca gaccgggtct gagacggctt gggaagacat tacgtcagac gtaaacggaa
     4141 aggccgacta ttttgctcta agaggctgcc tgcagttggc ggaccgggct ggtccagtca
     4201 gcgaagtgca tttgtccgaa ttcagggaca tggccgagaa tttttgtgct gggataaatg
     4261 caagggcgag atgcccggat gtacacaaag cgtatgcaca ggcgctggcc ctggatgaat
     4321 tctgcagtga agtcgatgtc atgataggca tcaccattat cagcagggac gggggtgcag
     4381 tgaccggggc aaagattcgc atggtggctg aagcgtcggg atttaagctt ggagcggacg
     4441 gcatgttcag cttccgtggt gaaaacggct cgacattgtt ttcactgggc aaccttgaac
     4501 cgcacccttt tttacccgat acgttgcgga cgctcaccac ccgtggcgta acttttctgc
     4561 tcgatgttcc caggacctcg aacggagaaa tggtttttga ggatatggtg cgggttgcgg
     4621 aagccttcgc ggattctgtc gggggaatca tggtggatga caacaacatt cccttgagtg
     4681 ccgagggcat cgaaaaaatt cagcagcagt tgatggctat cgaatccatg atggcgaccc
     4741 gaggtattcc tgctggcagc gaggtcgcgc gaaggctgtt tgcgtgaacc caattccgca
     4801 tcatctggcg gatcgcgcag gcgaattacg taagctgatc gaattgcaca actataacta
     4861 ctacgccctc gacgcgccta ctattcccga cgcggaatac gacacgctgt ttcgtgaact
     4921 gctcgacctc gagcaacagt accccgagct tgcaacgccc gactcgccta cccaacgggt
     4981 gggcctcgca gcgctcaagc aattttccca gatacaccac cggacgccga tgctctcgct
     5041 catcaatgcg ttcacagcgg aagatgtgga ggggttcgac cgtcgcgtac gccagcttct
     5101 atccgtcgac gaggttgaat atgcggtaga gcccaagttc gatggtcttg cggtcagcct
     5161 ctgctatgaa aatggcgtcc tgacatccgg ggcgacgcgt ggggatggat atgtcggcga
     5221 agacgtcacc cataacctgc gcacgataaa gtcaattccc ttgcagcttc acgctgatag
     5281 tcatgagcct ccccgcttcc tggaggtgcg gggggaggtg ctgatgttga gagctgattt
     5341 cgaaaagctt aaccagcacc agcgcgaaaa aaacgaacgt gaatttataa acccgcgtaa
     5401 cgccgctgcc gggtcgttac ggcagcttga tcctgctatc actgcaggcc gccggctgac
     5461 atttttcgcg catggcgtag gtgcatgcga aggtggaaag attccacgcg acaggcatac
     5521 gcatctcctc gattatcttg cttccaagcg atttccggtg cctcgcgaac gagacaccgt
     5581 gcgcggtttc cctgccctgc tcgaatacca tggcaagatc agccggatgc gtgaagcatt
     5641 gccttacgac atcgatggca ccgtatacaa ggtcaatgac ctggctcagc aggaaaagct
     5701 tggttttgtc tcccgcgctc cccggttcgc gattgcacac aagtttcccg ctcaggaagc
     5761 cgtgacggaa gtcctggcga tcgaggtgaa tgtgggcaga accggcgcat tgactcccgt
     5821 tgcccgcctg aagccggtat ttgtgggcgg ggtcaccgtg accaatgcaa cgttgcataa
     5881 tgaggatgag gtcaggcgca aggatgtgat gatcggggac agcgtgattg tccggcgggc
     5941 aggggacgtg attccggaga tcgtggctgt ccagaaggaa aagcgtcccc aggacgcaaa
     6001 attcttcgtc atgcctttgc attgccctat atgcggctcg aaggttgtac gcttgcccga
     6061 tgaagccgtc agccgttgta cgggcggatt attttgtccc gcacagcgaa agcaatccat
     6121 cctgcatttt gcttcccgca gggcgatgaa catcaaaggg cttggagaaa agctggtgga
     6181 tcatcttgtc gaccgaggca tcgtcaagac accggccgat ctttataaac ttggctcgac
     6241 ggatattgct gcattggagc gcatggcgga caaatccgcg gggaacatca tcaatgcgat
     6301 cgagaagagc aaggcaacga ctctcccaag gtttatctat gccctgggaa ttcgcaacgt
     6361 gggcgagacg acagcgagag atctcgcttc gcatttcggg gcgcttgatc gattgattgc
     6421 cgcggacatt caaagcctgc agcatgttcg agatattggc cctgtcgtcg ccgaaagtgt
     6481 cgcgaacttc tttgctgaaa ggcataacct ggaagttatc gaccagttgc gggaaagccg
     6541 ggtacgatgg gaggaaagcc agagtaccga gcaggctgcc cccggtgggg cagctgagct
     6601 cttaggcaga gttgcaggca ggactttcgt gttgaccggg acgctaccgc atttgagccg
     6661 tgaagaagcg agggagcgta tcgaagccga gggggggaag gttgtgggaa gcgtatcgaa
     6721 aaaaacggat tacgtggtag caggggatga tccgggtagt aaatatgaga aagctgttga
     6781 actcggaata acgattcttg atgaggaagg gctgttgaat ctattgcaga catcctgagg
     6841 ggttttataa gggggaccga ggtatgccaa aaataaccaa ggcggttttc cccgtggcag
     6901 gaatgggcac ccgttttttg cctgctacaa aggcaagtcc caaggaaatg atgcccgtag
     6961 tcgataagcc tttgatccag tatgcagcgg aagaagctgt agcagcagga ataagggaac
     7021 tgatctttat aacagggcgt cataaacgtg caatagagga tcactttgac aaagcttacg
     7081 aaatggaagc ggagttggaa gcacggcata agtacgatac cctggctatc ttgcggagta
     7141 ttttgcccaa ggacatctcg tgcatctata tacgtcagcc agaggctctt ggcttaggcc
     7201 acgccataag tctctcacgc cccgccgtag gcaatgagcc atttgcggta ttgctcgccg
     7261 atgatctgct cgtggggaac acacccgtaa tgcagcaatt ggctgaaata tacgaacaga
     7321 acaattgctc gatacttgca gtcgaaaata tcgaacgcga ggaaacatcg aattacggta
     7381 ttgtaaaatg cgaacccgga atgaagaggg tgcatcccgt aacagggatt gtggaaaaac
     7441 ctcggcctga ggatgcgcct tcaacgcttg gagtggtggg acgttacatt ctgactcccg
     7501 gaatttttcg ccatctcgaa aaaatccggc cgggcgcggg aggagaaata cagcttaccg
     7561 atggtattgc ttcactgctg gaggaggagc gggtgctcgc ttatgaattc cgtggcatcc
     7621 gttatgattg cggaagcaag cttggatacc tgaaagccac ggtcgcgatg gcgctcgagc
     7681 atcccgaagt ggcgcagccg tttgccgagt acctggccac gctaccatgc tgccggcctc
     7741 ttgcttcatg ataatgctaa cgagttgccg gctggtgcag ccggcatgag tcttcactcc
     7801 agcgttgctg ctctcggtca ttcgcccatc tacatttatt tgcacccctt gcggcatgct
     7861 gaccttcgaa gaagccaata acataatgag ctcagctgag ccgctgtttt cagcccagga
     7921 gattgcgaag acgataaggc gcatggcgac cgaaatcacg gtggcgctgg cgaatgacta
     7981 tccggtggta ttgagcgtaa tgggtggcgc tgtcgtattt acgggccatc tcctgccttt
     8041 gcttgaattt ccccttacgt tcgattatct ccacgtcagc cgctatgcca atacgagaca
     8101 tgggggagaa atccgatgga aaatgcgtcc gcaggaagat attcgcggcc gcgtggtgct
     8161 agtgctggat gatattctgg acgaaggggt gacgctcgca gcaatacgcg actgggcagt
     8221 gagtcgcggc gcatccgggt tttatagcgc tgttctcgtg gaaaaggaga ttgggcgacc
     8281 gaagccgatt catgcggatt ttaccggaat aacgttgccg gatcgctatg tatttggttt
     8341 tggaatggat ataaacggtg catggcggaa tctgccggcg atctatgcgt tgaaggacaa
     8401 gggtttccca aagacggagc agcccttttc taatcagttt tgagctcagg aacgtctcaa
     8461 caagtcctcg cggagtgcgt tgctgataaa atcgggatga tcacaaggcg cgcgacgaag
     8521 gtcgtagcgg ggactacgat gccaaggagt gcaacgcagt ggcgccccgg agcgttgccg
     8581 agggaccccg cagtgcgagg atcggcagcg gaggggatgt ccgaccgtac aaggtataat
     8641 gaagggcgcc cacatcccgc tatgggatcg caccttccga cggaagaacc tgctccgccc
     8701 tacgcggtcg tgccattcgc ttcgtcgcct ttcgcgcacc ccatctcgca aagccgtcgt
     8761 cgcgcccacg atgacttatt cagaggtttc ctacgttatg cttgcaatta taggcggtac
     8821 gggaatggct cagctaccct gcctggagat atcgcacagg cagattatca ggacacccta
     8881 tggagaacca tccggcccgt tcaccttcgg gagcataaac agtaacaagg tcgtattcct
     8941 tgcccggcac ggctacggcc atacaattcc cccgaacgag gtaaattacc gcgccaatct
     9001 ttgggccctg cgttcactcg atctcatgaa tgtcgtggcg gtcgcctcgg taggcggaat
     9061 acgtgccgac ctggcgcctg gcgtgctcgc cgtgcccgac cagattatcg attacacgca
     9121 tggccgtcac tatacttatt atgaaggtac agacaggcct gtcacgcaca ttgactttac
     9181 cgaaccctat tgccagtcaa tgcgaaatcg cctcctcaag gcggcgctgc ttgcgggcga
     9241 gaaagtcatc gatgggggtg tctacggggc aacacagggt ccccggctgg aaacggcagc
     9301 tgagatcaac cggatggaac gggatggggt cgatatggta gggatgaccg gcatgccgga
     9361 ggcatcactg gccagggaac tgggcttgtg ctacgccact gttgccgttg tcgcaaacta
     9421 cgctgccggc cgcaagacga gcgcgcattc cattagcctg gaagacgcgc actccgtact
     9481 cgaaagggca atggtgaggg tgcgggatat tcttgaaaga gtggtggaaa ccgatggcga
     9541 ttagaccggt tttgaagatg ggagacccgc gcctgctgga ggttgctgag aaagtcaaaa
     9601 gcttcgggac accggcgctc cataacctga tacgcgatat gcgggatatc atggaatcgc
     9661 tcgacggcgc gggtttggcc gcccctcaaa tcggggttgg cctgcaggtc gtgatattcg
     9721 gcgtaaagcg taatccgcgc tatcccgacg ccgaggaggt gccgtatacc gtgctggtta
     9781 accctgtcct gactccgctt accgatgaga tggagccagg ctgggaggga tgcctgagcg
     9841 ttccggggtt acgcggaatg gtcccccgct ttgccagatt gcgctacgaa ggatcggatc
     9901 agtacggcag acgtatcgag cgcaccgtgg aagggtttca tgcgcgggtt gtgcagcatg
     9961 agtgcgacca tctacaggga atcctttatc ccatgcgtat ttccgatttt cgcacgttcg
    10021 gttttaccga tgtgctgttt ccggccgagg cgccgatgga ggaataggcc cggtaaagaa
    10081 gccttgatcg agcttttcgc gccaaaaaag gccattattc agcctgaata tcttggtatc
    10141 cgagtatggt gggaagacaa aagagaggca ggggcaggga aaatatgcgg aatcagttcg
    10201 ttccgaagtt atccgttatt ttcatccgtt gtgcgacctg aggccggagc aggccaactg
    10261 ttgggccaat agctgagcca ggtggcgcgc gctccgtttt ccgctttcga caacaatgtg
    10321 ggcagcgtcg cgataaagtg gatcgcgctg agcgtaaagt tccgttaatt tcttacgcgg
    10381 gtccaccgtc cttagcaaag gcctgttctg atcctgccgg gtgcggcgcc acaaatcctc
    10441 gacggccgct cgcaaataga tgacgattcc tctctgcttc agaatcctgc agttttctgt
    10501 gttaagcacc gagcccccac ccgtggcgag tacgatgttt tccatttgag ccaactcggc
    10561 cagcatgtcc gtttcgcgtt ttcggaagcc ggcctcaccc tcgatttcaa aaatcaacgg
    10621 tatcccgact cccgttcgct tttgaatctc acggtccgag tcatagaatg tcttgccgag
    10681 aaaatgggca agaagtctgc ccactgttgt cttgcccgct cccatcattc cgatgagaaa
    10741 gatattgccg ctcgcctcgc cgggcacctc atcttccgcc attttcatgt tgagcgtttc
    10801 cataggccaa ttgtagctga aatgccacaa ttcgcgtgga aacatctcct tatcccgtct
    10861 cgcaggctaa cctgaccggt cagattctgc gagatcgcat gaggtgcagc acagagggta
    10921 gggctcggtt aatgagtgac gacttcagct cgttccgcaa tatccccgcg gtaatcgcgg
    10981 ccccgccctt tgctgccttt ttgagtgatc cgtgcggcat cgcggcaatc agcccgagaa
    11041 cggccagaga ggtaaaatat gggtggcggg ttatgaactt gaacgtatgg ctgcgcggaa
    11101 atgtgtccgc gacggaacgg tgtagatgct ctttctcatt ctcgtcctcg atgttgagca
    11161 ttcgccggta gtttttgcgg gtggcatcca ttcgctcaag gatcgccatc ctttcctcct
    11221 cgagagagct tttcatacca cgtccttcat gaatttcacg tcctgttgca attcgttacg
    11281 aagagtatcc agagcggaaa ccgaatcgtt ccgcgccggc atgctcagat aaatgatgaa
    11341 cgctatcaac gcgtagaatc cggccacccc ccaggcggac atgacacgat aaggggtatc
    11401 ccaaaaggta acaatgacag cggcgccgag aaacatgagg ctcaaggcag caaacagtcc
    11461 ggcgacgacg gcgccgatca cgcgcttgat cagactctgg ccctgaatct ctgcggatac
    11521 cttgaacaat tccacataat cgcccaagcg ctccaaagca attgctccga gctgttgggc
    11581 tttttcgagg gtagcaaaca tgatggattc tcgaccaagc cagggaaaac gatcagcggc
    11641 gcgtcatcag cagacccaga agaaagcccg ccgcggccgc gtatcccacg gattccagcg
    11701 ggcggtcttt tacgtactcc ttcgagcact ccacgccatg ctgaaattgc tcgcgggcat
    11761 tccccgcaat gcttttggcc gattctctgg tctccgcaat tttctgcatc agcttttcct
    11821 tggcgtcttc caggtcaacg tcggatagcg tcggaatctt cgcgatgagg tcatcgagat
    11881 cagccctgag atttgaaagc tcttcacttg cgacggcacc cacgtgtttg cctgccgatt
    11941 tggctgcttc accaacccgt tgagcagctg accccacgtg atgcgccatg ttttccgcag
    12001 cgccctgtgc atcgtctgtt ttgttcgcgg aagtatctgt ctgcataatg gactccttgg
    12061 ttcgattaga aaaaaacatc gtacaaatcc gtgcaggcaa ggctcatttc cctacctcag
    12121 cctcatgcct caacctcatc ccattctccg caattaatca gccatgtatg tacgttgaca
    12181 cacatagttg ctcccgtttc tcagtttagg gatgccgctg cgccatgcct taaaaatgga
    12241 agagccagag cggaaactcc ttccccggtt caaattgagt gagcaaagtc aaagatgata
    12301 taatccgcaa acttggcctt ctggtacctt ttcgaggttt gccggagttg atctcgactg
    12361 tgaaggcctg gtcaaaacgc atatccgcct ttcataaggg tgttccgacg gaacgaacgt
    12421 ggcggg
//
LOCUS       DASSHJ010000243         5993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_668552, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000243 DASSHJ010000000
VERSION     DASSHJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668552"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFI43_09435"
     CDS             complement(<1..676)
                     /locus_tag="VFI43_09435"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7062387.1"
                     /translation="MIYSKVIGTGGYLPEKVLTNHDLEGMMDTSDEWIRTRTGIVERH
                     IAAENEMASDLALNASRGAIEAAGIVPADIDLIIVATTTPDMIFPSTACILQHKLGVR
                     DGPAFDMQAVCSGFIYALATADMYIRAGRARTALIVGTEIYSRILDWTDRSTCVLFGD
                     GAGAVVLSRSEQPGLISSHLHADGSYSGILTAPGRFSGGKAQGNPFVTMEGSTVFKFA
                     VKVLEEV"
     gene            complement(673..1737)
                     /gene="plsX"
                     /locus_tag="VFI43_09440"
     CDS             complement(673..1737)
                     /gene="plsX"
                     /locus_tag="VFI43_09440"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7062388.1"
                     /translation="MNITVAVDCMGGDHGPHVTVPAAVNYLKRNPETEIILVGLPDVI
                     GAELGAINFSPNPRMRVEAASEVVGMDESPTLALRGKKNSSMRVAVNLVKSGEAQACV
                     SAGNTGALLATSRFVLKTLPGIDRPALAVVLPTIRGHTYVLDLGANVDCTAEHLLQFG
                     VMGATLVSSVENKERPSIGLLNIGQEEIKGNEVVKQAADLLRDSGLNFYGNIEGNDIY
                     KGTTDVVVCDGFVGNVALKTSEGVAQMLSTFLREEFKRNLLTKFAGLVAIPVIKAFKR
                     RIDHRRYNGASLLGLRGIVIKSHGSADTHAFGFAIARAVEEVRGGMLHHIRERIAQLP
                     HHLPKSAVYSPSPGVEGGRA"
     gene            complement(1801..1980)
                     /gene="rpmF"
                     /locus_tag="VFI43_09445"
     CDS             complement(1801..1980)
                     /gene="rpmF"
                     /locus_tag="VFI43_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7062389.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLSNPPLTMERGTGEVHLRHHISP
                     NGFYRGRKVIKAKGD"
     gene            complement(2012..2512)
                     /locus_tag="VFI43_09450"
     CDS             complement(2012..2512)
                     /locus_tag="VFI43_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET7062390.1"
                     /translation="MPVRHSIDALDFARNAGTCHGRIPLSKLERLQDYLIENSGELRY
                     EVSGALNNNGKPILKVSIQGVLKLRCQRCLDELEHLLDVKADLRLAENERELSSLDED
                     ESVEGILAEPHLDVLVLIEDEVILSLPISPRHEVCERSASLTSDPETAVDKPLYAALA
                     ALKNLH"
     gene            2814..3410
                     /locus_tag="VFI43_09455"
     CDS             2814..3410
                     /locus_tag="VFI43_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HET7062391.1"
                     /translation="MKTQEIAQELVLGSSSIYRHELLSRLQIPFQIFNPEVDETPLQH
                     ERPESTALRLAENKARSVAVNFPGALIIGADQAAALGGLQLGKPMNRINAVSQLRAVQ
                     GKEVTFFTALSLFNGRKNQGQTRIVESHVKFRKLTHQQIENYLDKEQPYHCAGSAKAE
                     GLGIALIERIVSDDPSALIGLPLIALVEMLGNEGVEVI"
     gene            3407..4111
                     /locus_tag="VFI43_09460"
     CDS             3407..4111
                     /locus_tag="VFI43_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7062392.1"
                     /translation="MSTGILYLIPVPLGNGEISSILPDSVRRRALELDYFVVEHPKAA
                     RKFLKQAGCTRLQDIRMSVLDEHTPASEIESLLEPLLSGSDGGLLSEAGCPGVADPGA
                     DLVRLAYKKNIRVVPLVGPSAILLALMASGLNGQQFAFHGYLPVDRSALVQKLLELEK
                     ESQAHDQTQIFIETPYRNQKLLACILSICRDNTLLSVSSDLTTADEFICTKTVSEWRH
                     NLPNLDSKPSVFLLHA"
     gene            complement(4144..5094)
                     /locus_tag="VFI43_09465"
     CDS             complement(4144..5094)
                     /locus_tag="VFI43_09465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET7062393.1"
                     /translation="MADSGNSGEGWEKRVLEKLALSSLQEQRRARRWSVFFKSLTFVY
                     LSIFLFYWLGWFPESDMSISGKHTALVDLRGVISPDTISSADNINAGLQHAFKDRRTK
                     GVILRINSPGGSPVQAGYIADEIRRLRAEYPDIPLYAVVEDICASGGYYVAAVADKIY
                     VDKASVVGSIGVLMNGFGFTGTMEKLGVERRLLTAGENKAFLDPFSPLNNKQEKYAKE
                     MLNEIHQQFIDVVRQGRGERLKDDPDLFSGTVWTGERSIQLGLADAVGSSDYVAREII
                     KAESIVDYTTREGFAERFAKRFGGVVAEVLLSSGKGWGLQ"
     gene            complement(5285..>5993)
                     /locus_tag="VFI43_09470"
     CDS             complement(5285..>5993)
                     /locus_tag="VFI43_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7062394.1"
                     /translation="FEVLFEDHCLIVINKPSGMAVHGGSGISLGIIEQLRAQHPEWKF
                     LELVHRLDRETSGVLLLAKTRTALVELHRQIREGVMGKHYLTLVKGEWQNARQSVRLP
                     LNKFVTLAGERRVAVVTGTKVEKPMAAHTVFVLKRRFQDFSLLQAELKTGRTHQIRVH
                     LSHLGFPIAGDDKYGDFSLNKQLAKRTRSSPLLRMFLHAASAEIAHPVTGEQLRLEAP
                     LPQDLRDFLKSLDAEND"
BASE COUNT         1413 a         1732 c         1431 g         1417 t
ORIGIN      
        1 cgacctcttc cagtaccttc acagcaaact tgaagactgt actgccttcc atggtaacga
       61 acgggttgcc ctgcgccttc ccgccactga agcgcccggg tgcagtaagt atcccgctat
      121 agcttccgtc ggcatgcaga tgacttgaga tgagcccggg ctgttcgctc ctgctcagga
      181 caactgctcc cgcgccatca ccgaagagca cacacgtact ccggtccgtc cagtcaagta
      241 tgcgcgagta tatctcggtg cccacaatca gcgcggtgcg cgccctgccc gcccggatat
      301 acatgtcggc ggttgcaaga gcgtagataa aaccgctgca gacggcttgc atgtcgaaag
      361 ctggcccatc ccttacgcca agcttatgct gcaaaataca tgcagtgctt ggaaaaatca
      421 tatcaggtgt cgttgtcgca acgatgatca ggtcaatgtc agctgggaca atgccggcag
      481 cctcgatcgc gccgcgactg gcgttcagcg ccaggtcgct tgccatctca ttctcggcag
      541 cgatgtgtcg ctcaacaatg ccagtacgcg tccttatcca ttcgtcgctc gtatccatca
      601 tgccctcgag gtcatgattt gtaagcacct tttccggcag atatccaccc gtcccgatga
      661 ctttagaata aatcatgcac gacccccttc aacccctggg gacggggaat agacggcgga
      721 ttttggcagg tggtgcggca attgggcgat ccgttccctg atatgatgga gcattccacc
      781 acgaacctct tccaccgccc gagcgatcgc aaaaccaaac gcatgggtat cggcagaacc
      841 atgacttttt atgacaattc ccctcaaccc gagcaaactc gccccattgt agcgccggtg
      901 atcaatgcgg cgcttgaaag ccttgatcac gggaattgcg acgagccctg caaattttgt
      961 caggaggttg cgcttgaatt cttcacgaag gaaggtagag agcatttgcg caacgccttc
     1021 cgacgttttc agcgccacat tgccaacgaa accgtcacac acaaccacat ccgttgttcc
     1081 cttataaata tcattgccct cgatattgcc ataaaaattg aggccgctat cccgcagcaa
     1141 atccgccgct tgcttgacga cttcattccc cttgatctct tcctggccta tattgaggag
     1201 cccgatgctg ggcctctcct tgttttccac tgaggacacc aaagtagctc ccataacgcc
     1261 gaattgaagc agatgctccg ctgtacaatc cacgtttgca cccaagtcaa gtacgtaggt
     1321 gtgccccctt atggtaggca gcaccacggc gagagccgga cgatcgatgc cgggcagcgt
     1381 cttcaatacg aaccgcgagg ttgcaaggag agcgcctgta tttccagcgc ttacgcaagc
     1441 ctgggcttct ccgcttttga ccaggttgac cgcaacccgc atcgaggagt tttttttacc
     1501 gcgcaacgcc agggtgggcg attcatccat gcccacaacc tcgctggctg cctcgaccct
     1561 catccgaggg ttgggcgaaa agttgattgc gcccaattct gccccgataa cgtcgggcag
     1621 acccacaaga atgatctcgg tttcgggatt ccgtttaagg taatttacgg ctgcgggaac
     1681 cgttacatga ggaccatgat cgccccccat gcaatctacg gctacggtta tgttcaattt
     1741 ttagcgccct ggaggcaggg aaagctgaaa aacttcgatc cctaaaggat ggggcgagat
     1801 ttagtcccct ttcgctttga tgacctttct tccccgatag aatccgtttg gactgatgtg
     1861 atggcgcaag tgcacttcac ctgtcccccg ctccatggtt aatggcgggt tgctcaagaa
     1921 atcgtgcgat cggtgcatgc cgcgctttga cggcgacttc ttgttttgtt ggactgccat
     1981 ggtcaactcc ttaaagtagc atctattgca atcaatgcag atttttcagc gccgccaaag
     2041 cggcataaag tggtttatct accgcggttt caggatcaga cgtaaggctg gccgagcgtt
     2101 cgcacacttc gtggcggggc gaaatcggca ggctcaaaat gacctcgtcc tcgatgagaa
     2161 ccagaacatc caggtgaggt tcagcaagaa tgccttctac agattcatcc tcatccaaag
     2221 atgaaagttc cctctcattc tccgccagcc gcagatccgc cttcacatcc agcagatgct
     2281 cgagctcatc cagacagcgc tggcacctga gcttcagtac gccttgaatg gaaactttca
     2341 ggatgggctt gccattgttg ttcagcgcgc ccgaaacctc ataccgcaac tcgcctgagt
     2401 tctcgattaa ataatcttgc aagcgctcaa gcttggaaag aggaattcta ccatggcatg
     2461 tcccagcatt cctggcaaaa tcaagcgcgt caatagaatg ccttacgggc atgatgagct
     2521 tttcaataat tacgtgattc gggatttgga gttacttatg caaacggcat gacgtcatca
     2581 agacgagcct tactctcctc tgtcttagga cgcggaaaaa cagctgccgt tcaccattcc
     2641 aaaaaacatc aataagataa atatccttac agaagaacct cggcgcaaga gccggaacgt
     2701 tcttaaattg ataaataatt ccactggtat taaaattcca tacgcattgc acggcgcgca
     2761 tcatatattt tttgcgcatg cgtgtcaaaa acacaaggcc agaaaattaa actttgaaaa
     2821 ctcaagagat tgcccaggag ctcgttttgg gctccagttc catttatcgc cacgaactcc
     2881 tcagtcgcct gcaaattccg tttcaaatat tcaatccgga agttgatgaa acacctctgc
     2941 aacacgagcg tcccgagtcc accgcactcc ggttggcgga aaacaaggct cgttcggtcg
     3001 cagtgaattt tcccggcgcg ctgatcattg gtgccgatca ggcagcggcg cttggaggct
     3061 tgcaactcgg caaaccgatg aacaggatca atgcagtgag tcagctacgc gctgtccagg
     3121 gaaaggaagt gacttttttt accgcattga gcctttttaa tggccgcaag aaccaggggc
     3181 agactcgtat cgttgaatcc catgtgaaat tccgaaagct gacccaccag caaatcgaaa
     3241 actacctcga taaggaacag ccctatcact gtgccgggag tgcgaaggca gaaggactcg
     3301 gtatcgcgtt aatcgagcgc atcgtgagcg atgatccgag cgccttgatc gggctgccgc
     3361 ttattgcgct cgtcgaaatg ctcgggaacg aaggtgtcga agtaatttga gcactggaat
     3421 cctctatctc attcccgttc cgctgggaaa cggtgaaata agctcgatac ttcctgattc
     3481 agtccgtaga cgggcgctgg agctcgacta ctttgttgtt gagcacccta aagccgcaag
     3541 aaaatttctc aagcaagcgg gatgcactcg attacaggat atccggatgt ccgtactgga
     3601 tgagcatacc cctgcgagtg aaatcgaaag cctgcttgaa ccattgcttt caggaagcga
     3661 cggaggactg ctatctgaag caggttgtcc aggcgtcgcc gatcccgggg cagacctggt
     3721 gcggctcgct tataaaaaaa acattcgcgt ggtgcccctc gttgggccct ctgccattct
     3781 gctggccctt atggcttccg gcctgaacgg ccagcagttc gcgtttcacg gatatcttcc
     3841 cgtcgatcgg agcgcgcttg tccagaaact gcttgaactt gaaaaagaat cgcaggctca
     3901 tgatcagact caaatattca tcgaaacacc ttaccgcaac cagaagctcc ttgcatgcat
     3961 cctgtcgatc tgccgcgaca atacgcttct ctccgtttca tccgatctga caacggctga
     4021 tgaattcatt tgcacaaaaa cggtgagcga atggcgtcat aatttgccga atctcgacag
     4081 caagccttcc gtttttttgc tgcacgccta atgctccaga aagggtttcc gcaaaacgag
     4141 atttcactgc aatccccacc ccttgcccga gctcaacaga acttcggcca cgaccccgcc
     4201 gaagcgcttt gcgaaacgtt ccgcgaaacc ttctctcgtc gtgtaatcta caatagactc
     4261 ggccttgatt atttcacgcg caacgtaatc cgagcttccc actgcgtcgg caagacccag
     4321 ttgaatactt ctctcacccg tccacactgt cccgctgaat aggtcgggat cgtccttgag
     4381 gcgctcgccc cgcccctggc gaaccacgtc aataaattgc tgatgtattt cgttcagcat
     4441 ctcctttgcg tatttttcct gcttgttatt caacggagaa aagggatcaa gaaacgcctt
     4501 gttttcccct gccgtgagca atctgcgctc aacacccagt ttttccattg tgccagtaaa
     4561 gccaaatccg ttcattaaca cgccgatcga tccaaccaca ctggctttgt ccacgtatat
     4621 cttgtccgcc accgccgcca cataataccc gccggaagcg caaatatcct cgaccactgc
     4681 atacagagga atgtcgggat attcagcgcg aagacgtctt atttcatcgg caatataacc
     4741 cgcttgtaca gggcttcccc cggggctgtt gatccggagt atcacgccct tcgtccttct
     4801 atccttgaag gcatgctgca agcccgcatt gatgttatcg gcactgctga tcgtgtcggg
     4861 ggaaatcacg ccgcgcagat caaccaatgc agtatgtttc cccgaaatcg acatatcgct
     4921 ctcaggaaac cagcctagcc agtaaaaaag gaatatcgat aaatagacaa aggtaagaga
     4981 cttgaaaaaa acgctccagc ggcgtgcccg cctctgctcc tgcagcgagg aaagcgccag
     5041 tttttcgaga acgcgctttt cccacccttc gccagaattt cctgaatcag ccatataaac
     5101 ccttccattg aatcctcgaa cctgtcataa cacttattcc tctcggattt ccctctccgg
     5161 attgggcgtg atgccaatac gaagcgcaag ctacgcttca aagcgaagcc cttgcgctca
     5221 acatcgcccc gcagcttgag cgtataaggg cctatccatg gttgtccatc cttgtctgtc
     5281 agagtcagtc gttttccgcg tccagcgatt tcaaaaaatc ccgcaaatct tgggggagcg
     5341 gagcctccag gcgaagctgt tctcccgtta caggatgcgc tatctctgcg ctagcggcgt
     5401 gcaggaacat gcgtagcagc ggcgagctgc gggtacgttt cgccagctgt ttatttagcg
     5461 aaaaatcgcc gtacttgtca tccccggcta tagggaagcc aaggtgagaa agatgaaccc
     5521 ggatttgatg agtccgcccg gtctttagtt ccgcctgaag cagactgaaa tcctgaaagc
     5581 gcctctttag cacgaacacg gtatgcgcgg ccatgggctt ctccaccttt gttccagtta
     5641 ccacggccac acgcctttcc cccgccaagg ttacgaattt gttgagtggc aacctgacgc
     5701 tttgcctggc gttttgccat tcacctttaa caagcgtcag gtagtgcttc cccatgactc
     5761 cttcgcgtat ctgcctgtgc agctcaacga gcgccgttct tgtctttgca aggagcagaa
     5821 ctcccgaggt ttccctgtct aggcgatgca ccagttccag aaacttccac tcgggatgct
     5881 gcgcccgtag ctgctcgata atccccaggc ttattccact tccaccatga actgccatcc
     5941 cggatggctt gttgatgact atcaggcagt gatcctcgaa cagcacctcg aac
//
LOCUS       DASSHJ010000244         9062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_591478, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000244 DASSHJ010000000
VERSION     DASSHJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591478"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFI43_09475"
     CDS             <1..496
                     /locus_tag="VFI43_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062395.1"
                     /translation="DDQLAVAVAQACVTAGYCGVVRHIIEQSLDRQWNPELARLYGEC
                     PEKDAIGQIERAEGWLASHPGDAGLLLALGKLCIQQELWGKAQNYLDASLSIDRDYPA
                     HLALAQLNEKIGQARLAKDHYDRGLALALHRLKVLGHKHLVDENGSGDDLRSSGLGTH
                     VLDS"
     gene            complement(522..1562)
                     /locus_tag="VFI43_09480"
     CDS             complement(522..1562)
                     /locus_tag="VFI43_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HET7062396.1"
                     /translation="MSHQITIKPSGHTFIAQPGETVLQAALREGFPLPYGCRNGACGT
                     CKGKIVQGTVDFGNYTESALSEAEKHAGMALFCCAMPLSELVIECREIGAIKDIKVKT
                     LPCRVQKLERIAPDVMIIYLKLPANERLQFLAGQYIDILMKDGKRRSFSLANAPHDDE
                     LLQLHVRNYLGGTFAEHVFTHMKERDILRFEGPFGTFFLREDSDKPIIFIASGTGFAP
                     VKSILEHVFRDRNPRSSARKMTLYWGNRTRADLYMPDLPEGWQREHDNFAFIPVLSDA
                     SPNDNWNGRTGFVHHAVLQDFDDLSGFQVYACGAPAMVEAAHRDLTSLRGLPEEEFFS
                     DAFTASRLSPGS"
     gene            1622..2536
                     /locus_tag="VFI43_09485"
     CDS             1622..2536
                     /locus_tag="VFI43_09485"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7062397.1"
                     /translation="MSTRKRTLLIVGCGDIALRAAPLLQAHYRLVGLCRKPESRNKLR
                     LSGITPMHGDLDLPGSLGRISGLAHAVLHLAPPPEHGHTDSRTSHLLSALAKRPKIKG
                     AILPQQFVYISTTGVYGNCQGRFIDETCPVNPESRRAARRVDAERQIRAWGQRNGTNV
                     SILRVPGIYALDRLPLARLRQRECLALVPEEDSYTNHIHADDLARILVAALRHARPGR
                     IYNVSDDSRLKMGDYFDLVADHFELARPRRISRGQAEGGLISQNLFSFMRESRRLMNM
                     RMKHELNIDLRYPAVSNCLACPAPAEPG"
     gene            2625..2960
                     /gene="cutA"
                     /locus_tag="VFI43_09490"
     CDS             2625..2960
                     /gene="cutA"
                     /locus_tag="VFI43_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET7062398.1"
                     /translation="MNPILVITTMPDRASAISLAEALVDERLAACVNVLADCTSVYRW
                     EGKSEAATEVPVFIKTTEQNYSRLEQRIMSMHPYELPELIAVPISDGLPGYLKWIETE
                     TLTLDSKVE"
     gene            2986..4932
                     /gene="dsbD"
                     /locus_tag="VFI43_09495"
     CDS             2986..4932
                     /gene="dsbD"
                     /locus_tag="VFI43_09495"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HET7062399.1"
                     /translation="MRLIRYLFILSLGFLSLNAIAEEKASFGFLSGLSKLGMSADQDE
                     PELLPPDQAFKLTVKVRDENTLVAQFEPAENYYLYKDKVAFKPQDTATSIEQISLPRG
                     DLKSDVTFGEVEVFHRPFEALISLKRNSSSADKLTLVATYQGCNEPVGVCYAPINKVI
                     ELTLPAAKAAAGAIASAVSGEAVAASPLRTGAPDATAELFPSQPPAVETESLKIERMF
                     ETGNFWLILTGFFGVGVLLSFTPCVFPMFPILSGIIANRGEHITKTRGFALSLAYVAG
                     MAITYAAAGIAAGLSGAMLSAMLQNAWVLGSFALIFVALAFSMFGFYELQLPGFLQSK
                     LSEEAGHLKGGHLASVFGMGALSALIVGPCVAAPLAGALLYISQTRDVVLGGSALFAM
                     ALGMGVPLLLLGASAGALLPKAGPWMESIKQFFGVLLLGVAIWLISPVIPPVVHMLLW
                     AALLIISAIYLHAIDPLRPGASGLQKFLKGLGMISLLTGVALLIGVFSGSRDILQPLS
                     KISIASSDTANKNGSEAGRNGHLQFRKIRSIAELEQQVLQSRDKYVMVEFSADWCISC
                     KEMERFTFTDSKVQSRLKDVVLLQADVTAGTPDDQALLKRFKLFGPPGILFLDREGRE
                     VRGVRLIGFQDPDRFLAVLNAVLI"
     gene            5092..5637
                     /locus_tag="VFI43_09500"
     CDS             5092..5637
                     /locus_tag="VFI43_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7062400.1"
                     /translation="MTKSQQIVLYLGAALIAVASGFLLRGQLTSGSHVGTAADSAKGA
                     QTILAANLPDLNDKNQPLSQWRGKVMVVNFWASWCEPCRKEIPEFIELQEKFGERGLV
                     FVGIAVDQKERAAAFSKAIGINYPVLVGDMRAMMLAEAAGNRQGALPFTVVIDRNGNI
                     TGTKLGSLSRSKLESMFIPLL"
     gene            complement(5665..6174)
                     /locus_tag="VFI43_09505"
     CDS             complement(5665..6174)
                     /locus_tag="VFI43_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062401.1"
                     /translation="MKRKISIRYLVALALLALLIPLSEARDPAAEKSHQQDRNPSPPG
                     VKASRIASAGGNMSVQLPARGRAEDTELPPAAFEKLVIHAGFGWGIFSGTLYNGNSDY
                     TVTGVTVRFTPLGKGNGDAENSSGGNEYDLELNLEPLSKTALSMPIPSDNTLEYSWKI
                     TKARGYKTQ"
     gene            complement(6304..7281)
                     /locus_tag="VFI43_09510"
     CDS             complement(6304..7281)
                     /locus_tag="VFI43_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7062402.1"
                     /translation="MKIRNLDLTPNPINEEHQQLGIPPIEDPVSHPGAHPLLHAAMWF
                     AGLVMIALVLFLTGRFFVSNDWVGGWQIINETLGAKTFWSAVAVGFFAQVVDGALGMA
                     YGVTATTFLLATGASPAAASASVHIAEIFTTGVSGISHAKFGNVNKDLFVRLLVPGIL
                     GGISGAFLVTHIDGAVFKPYISAYLLLLGVYIVSKAFRPLRLRTKAPKHVGKLALFGG
                     FVDSAGGGGWGPVVTSTLVGSGNDPRTTIGSVNFAEFFLTLAGATVFTLLMENNTWTI
                     IVGLVFGGLFAAPFAAVLCKKLHAKTLLILVGVLIIMTSTYNLYRALSL"
     gene            7409..7783
                     /locus_tag="VFI43_09515"
     CDS             7409..7783
                     /locus_tag="VFI43_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HET7062403.1"
                     /translation="MLLADVILVIHFLFALFVVGSLPLIWIGSWMRFRFARNRSFRFL
                     HLMAIVFVASESLIGMVCPLTSLEDALRGTGNGGSFIQRWLHRILYYDFPEWILTVAY
                     VLFAFLVALTYILVPPRGRKRG"
     gene            7915..>9062
                     /locus_tag="VFI43_09520"
     CDS             7915..>9062
                     /locus_tag="VFI43_09520"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET7062404.1"
                     /translation="MSAASFLKRIAAQKRRLGIGAGILVGLVALYGLLGYFWLPGFIR
                     TKVESELSASLHRPVTVRSIEIQPYTLEFTVRGFRVGGKPSDSDAGNDLLSFEELYVN
                     VSGASLTKGAPVISAVTLRGPVVRLVREGENRFNITDLIEDFMKQPPSEGTTMFSVSN
                     ILVEDGSFTLIDQLKDSRQEISGIRLGVPFVANLKSDEQSWVEPHFSANVNGAPLRLN
                     GKLRPFSTNREATLELKLNDVDLTRVDEYSPVPVGIRLSSGYLDSNLVLTFSQADSEA
                     PKITLTGEAALRKLGIENHGTEIPYAAKIAEIQIKPVELNLNSANRSHVALALSDAVI
                     SHEGEEQPVLSLPSLKVSQSSIDLAHQSVTLGDITLDRIHAFLRRESDG"
BASE COUNT         2024 a         2386 c         2570 g         2082 t
ORIGIN      
        1 agacgaccaa cttgctgtcg cggtggcgca ggcttgcgtg actgccggat attgcggggt
       61 agtccggcac atcattgaac agagcctgga caggcagtgg aatcccgaac tggcgcggtt
      121 gtacggcgag tgtcctgaaa aggatgcgat cgggcagatc gagcgcgcgg aaggctggct
      181 ggcatcgcat cccggcgatg caggcttgct gcttgctctc ggaaaacttt gcattcagca
      241 ggagttatgg gggaaagcgc aaaattatct ggacgcgagc ttgtccatcg atcgcgatta
      301 tcccgctcac cttgcccttg cgcagttaaa cgagaaaatc gggcaagcgc ggcttgcaaa
      361 ggatcactat gacagagggc tcgcgcttgc cctgcatcgc ctgaaagtgc tggggcataa
      421 acatcttgtg gacgagaacg gatcaggtga cgatctgcgg agttccggat tgggcacgca
      481 cgtcctggat agttgacgtg ggggtgtcac gaatgcctgg gtcaggaacc cgggctcagc
      541 cgggaagcgg taaatgcatc cgaaaagaac tcttcttccg gcaaccctcg taaggaggtc
      601 aagtcgcgat gagctgcttc aaccatggct ggggcgccac aggcatacac ctggaaaccc
      661 gagagatcgt caaaatcctg caatacggcg tgatgcacga aaccggttct gccgttccag
      721 ttatcattcg gcgaggcgtc ggacaaaacg ggaataaaag cgaaattatc atgctcccgc
      781 tgccagcctt cgggcaaatc aggcatatat agatccgctc gagtccggtt accccagtag
      841 agggtcattt tccgcgcgct gctccgtgga tttctatcgc ggaagacatg ttcgaggatg
      901 cttttgaccg gggcgaaccc ggtaccgctg gcgatgaaaa taatcggttt gtcggagtct
      961 tcccggagga agaacgtgcc gaaaggaccc tcgaaacgca ggatgtcccg ctctttcatg
     1021 tgggtgaaga cgtgctcggc aaatgtcccc cccaggtaat tgcgtacgtg caactggagt
     1081 agttcgtcat catggggcgc attggcaagt gaaaagctcc tccgctttcc gtctttcatc
     1141 aggatgtcga tgtattgacc ggcaaggaac tgcaggcgct cgttggcagg cagcttcagg
     1201 tatataatca tgacgtcggg cgcgatccgt tccagcttct gtacccggca gggcagggtt
     1261 tttaccttga tatccttgat cgccccgatc tcgcgacact cgatgacaag ttctgaaagc
     1321 ggcatggcgc aacaaaaaag cgccatgcct gcatgcttct ctgcttcact caaggcggat
     1381 tccgtgtaat tgccgaaatc gaccgttccc tgcacgatct ttcccttgca cgttccgcaa
     1441 gcgccgttgc ggcagccata gggaagcgga aaaccttcac gcagcgctgc ttgaagcacg
     1501 gtttctccgg gctgagcgat aaaagtatgg ccgctgggct tgatggtgat ttgatgcgac
     1561 atcaatcagg atgagtcaac aaggacgagt caacaataat atgtcgaagg ctttgggcaa
     1621 aatgagtact cggaaacgga cactcttgat agttggttgt ggcgacattg cgttgcgcgc
     1681 ggcacctttg ctgcaggcgc attaccggtt ggtgggcctt tgccgaaaac cggagagccg
     1741 gaataaactg cgactaagcg gcattacccc tatgcatggg gatctggacc ttccgggtag
     1801 cctggggaga atttcgggtc tggcgcatgc agtgctccat ttggcgccac cgcccgaaca
     1861 tgggcacacc gattcccgca cttcccatct tctgtctgcc ttggcgaagc ggccgaagat
     1921 aaagggggca atactaccac aacagtttgt ttacatcagt acgactgggg tctatggaaa
     1981 ctgtcagggg cggttcatcg acgagacttg tcccgtaaat cccgagagcc ggcgcgcagc
     2041 gcgacgggtg gacgccgaga ggcagatcag ggcgtggggg cagaggaacg ggacgaacgt
     2101 ttccatcctt cgcgtgcccg gtatttatgc tctcgatcgc ttgccgcttg ctcgccttcg
     2161 ccagagggag tgtctggcgc tcgtgccgga agaagatagc tataccaacc atatccacgc
     2221 cgacgatctt gcgcgcattc ttgttgccgc attacgccat gcacggcctg ggcgaattta
     2281 caatgtttcc gacgactcca gattgaaaat gggtgattat tttgacctgg tagcagatca
     2341 tttcgagttg gcccggccgc ggcgcatcag tcgcggccag gcggagggcg gattaatatc
     2401 tcaaaatttg ttttcgttta tgagagaatc ccgccgattg atgaacatgc ggatgaaaca
     2461 cgagctgaat atcgacctcc ggtatcctgc cgtttctaac tgccttgcct gtccggctcc
     2521 cgctgagccg ggttgaggtg tgttcctggc actacggctt tcttcattct ttgcgatagc
     2581 gattaaattt ccacgaactg ccagcctaaa aaacggtcgt tccagtgaac cccatactcg
     2641 tcataacgac aatgcccgat cgggcgagcg cgataagcct cgccgaagct cttgtcgacg
     2701 aaagactcgc agcctgcgta aatgtgctgg ccgattgcac ctcggtctac cgctgggagg
     2761 gaaagagcga agcggcgacg gaagtgcctg tgtttattaa aacaaccgag cagaattact
     2821 cgcggctgga acagcggata atgtcgatgc acccctacga actacccgag ctcatcgctg
     2881 tcccaatctc agacggtttg ccgggttatt tgaaatggat cgaaacagaa acactgacac
     2941 tggattcaaa agttgaatga acaatatcgg gttccttaag ttgcgatgcg tctgattcgc
     3001 tatttattca tcctgtcact tggttttttg agccttaacg ctatcgcgga agagaaggcc
     3061 tctttcggct ttctttcggg gttgtcgaaa ctaggcatga gtgccgatca ggacgagccg
     3121 gagctgctgc caccggatca agccttcaaa ctgaccgtca aggtgcgcga cgagaatact
     3181 cttgtcgccc agtttgaacc ggccgagaat tattaccttt acaaagataa ggttgctttc
     3241 aagccacagg atactgccac ttcgatcgag caaatttccc tgccccgggg cgatctgaag
     3301 agcgacgtga cttttggcga agtcgaggtt tttcaccggc catttgaagc ccttatctcg
     3361 ctgaaaagaa actcatcttc ggcagacaaa ctgacgcttg tcgcaaccta ccaagggtgc
     3421 aatgaaccgg taggcgtctg ttacgcgccc atcaacaagg ttatcgaact gacccttccg
     3481 gcggcgaagg cagcggccgg agcaatagcg tcggcggtca gcggcgaggc cgtggcggct
     3541 tcgccgctca gaacaggggc gccggacgcg acggcagagc tgttcccatc tcagcctccg
     3601 gctgtcgaaa cagagtcatt gaaaatcgag cgaatgttcg agaccgggaa tttctggttg
     3661 attctgaccg gtttcttcgg tgtcggcgtg cttctctctt ttacgccttg cgtcttcccg
     3721 atgttcccca ttctttcggg catcatcgcc aaccgggggg aacatattac caagacgcga
     3781 gggttcgctc tatcccttgc ctatgtggcc gggatggcta tcacttatgc ggctgcaggc
     3841 attgcggctg ggctttccgg agccatgctt tccgcaatgc tgcaaaacgc atgggttctg
     3901 ggtagttttg cactcatctt tgtagccttg gctttctcta tgttcggctt ctatgaattg
     3961 cagttgccgg gctttctcca aagcaagctc tcggaagagg cgggccattt gaaaggcggg
     4021 cacctggcga gtgtgtttgg catgggcgcg ctgtctgcac tgatagtcgg gccatgtgtg
     4081 gcggctccgc tggcgggtgc gctgttgtat atcagccaga cccgtgacgt ggtgctgggc
     4141 ggctcggcgc tgtttgccat ggcgttggga atgggcgtgc cactgctgct attaggcgct
     4201 tcggccgggg cccttctgcc aaaagcagga ccgtggatgg agtcgatcaa gcagtttttt
     4261 ggagtgctgc tgctgggcgt agccatatgg ctgatatcgc ccgtgattcc tcccgtggtg
     4321 cacatgctgc tgtgggcagc gctgttgatc atctcagcca tttatttaca cgcgatcgat
     4381 cccttgcgac ccggtgcctc cggtctacag aaattcctga aggggctggg gatgatctcc
     4441 ctgttaaccg gcgttgcgct gttgatcggc gtattttccg gcagccgtga cattttgcag
     4501 cccctgtcaa aaataagcat tgcctcttcg gataccgcca ataagaacgg atcggaagca
     4561 ggccgaaatg ggcatttaca gttcaggaaa atacgctcga tagccgagct cgagcagcag
     4621 gtgctccagt ccagggacaa gtatgtcatg gtcgaatttt cggccgactg gtgtatctcg
     4681 tgcaaggaaa tggagcgttt cacctttacc gattcaaaag tgcagtcgag gctgaaagac
     4741 gtcgtattgc ttcaggcgga tgtgaccgcc ggaacgcccg atgaccaagc cttgctcaag
     4801 cgtttcaagc tattcggtcc ccccggtatc ctgtttctcg acagagaagg gcgcgaggtt
     4861 cgcggagtga ggttgattgg atttcaggat cccgaccgtt tccttgccgt actgaatgca
     4921 gtgctgatat aggttgtttc gcggatccca ttccattgtt gtatccgcga aacgcagata
     4981 acgatctgct tctccacccg ttttcacgca tatacccggg gcatggcggc caggattttt
     5041 cctcaaccca ccaaaaagaa aaatttccaa gaaaatttcc gggaaaattc aatgacgaaa
     5101 tcgcaacaaa tagtgctgta cctgggagct gcattgattg cggtagcaag cggattcttg
     5161 ctgcgcgggc aactgaccag tggctcgcac gtcggaactg ccgcggattc tgccaagggg
     5221 gcgcagacca ttctagccgc caatctgcct gacttaaacg acaaaaatca gccgctctcc
     5281 cagtggcgtg gcaaggtgat ggtggtcaac ttctgggcga gttggtgcga accctgccga
     5341 aaggaaatcc cggagttcat cgagctccag gagaaattcg gtgagcgcgg attggtattc
     5401 gtcgggattg ccgtggatca gaaggagcga gcggcggctt tcagcaaggc catcgggatc
     5461 aactaccccg tgctcgtggg cgatatgaga gcaatgatgt tggcggaagc tgcgggcaat
     5521 cgccagggcg ccctgccttt taccgtagtg attgaccgca atgggaatat cacgggcacg
     5581 aaattgggga gtctcagccg aagcaagctc gagtcgatgt tcatccccct actgtaggtt
     5641 tctggctggt gcagaagact tgcattactg cgttttatag ccgcgcgcct tcgttatttt
     5701 ccacgaatac tcgagagtat tatccgatgg aatcggcatt gacagcgcag tttttgaaag
     5761 aggctcaagg ttcagttcga gatcatattc attgccacct gaagaattct cggcgtcccc
     5821 gttccctttg cccagtgggg tgaagcggac agtgaccccc gttacggtat aatcgctgtt
     5881 gccgttgtaa agcgttccac taaagatgcc ccagccaaaa ccggcatgga tgacgagctt
     5941 ttcaaaagcg gccgggggca gttccgtatc ctctgccctg cccctcgcag gtaactggac
     6001 gctcatgttt ccccccgcac tcgcgatacg actcgcttta acacccggcg gggaagggtt
     6061 gcggtcctgc tgatgcgatt tctctgctgc cggatccctg gcctctgaaa gtgggatcaa
     6121 aagagccagc agcgcaagcg cgaccagata tcgtattgag attttccttt tcatttgaaa
     6181 agaccaactt ggatgtgcgg ggaacaatct tactcgtcga taattgacct gaatataatc
     6241 aacagttgtc tgcgagttgt cagtggcata tggatttgat cgagacggca aggtcgggat
     6301 ggcctagagc gacagtgccc ggtaaagatt gtacgtactg gtcatgatga tcaatacccc
     6361 cacgagtatg agcaacgttt tggcgtgaag cttcttgcac agcacggcag cgaacggggc
     6421 agcgaacaga cccccgaaca cgaggcctac gatgatcgtc caggtattgt tttccatcag
     6481 aagcgtaaaa actgtagctc ctgcaagtgt cagaaagaat tctgcgaaat tgaccgagcc
     6541 gatagtcgtg cgcgggtcat tgccagagcc caccagcgtt gatgtcacga cgggtcccca
     6601 gccgcctccg cctgcggaat cgacaaaacc gccgaacaac gcaagttttc cgacgtgctt
     6661 gggggccttc gtccgcaggc gtagcggccg gaacgccttg ctgacaatat aaactccgag
     6721 cagcaacaaa taagccgata tgtaaggctt gaacaccgcg ccgtcgatgt gggtgacaag
     6781 aaaagcgccg gatatcccac ccagaatacc gggcaccagc aagcgtacaa agagatcttt
     6841 attgacattg ccaaatttgg cgtgcgagat tcccgaaaca ccggtggtaa aaatctccgc
     6901 gatatggacg cttgcgctgg cggccgccgg actcgcgcct gtcgccagga ggaacgtggt
     6961 ggccgtaacg ccatacgcca ttcccagtgc gccatccacg acttgggcga aaaatccgac
     7021 cgcgactgcg ctccagaaag ttttcgcgcc gagcgtctcg ttaatgattt gccagccacc
     7081 cacccagtca ttcgatacaa aaaagcgtcc cgtcaggaaa agcaccagcg caatcatcac
     7141 aagtccggca aaccacattg cggcatggag aaggggatgt gctcccggat gggaaacagg
     7201 atcctcgata ggtggaattc cgagctgctg atgttcctcg ttgatgggat taggggttag
     7261 atcaagatta cgtattttca tggggttgtg tttgctaact cttctgtttc gcagacacta
     7321 taccgatagc cggaaccatg cagaagtaac cgaatagaat atgtttattc ttttgctgtc
     7381 tgcccgcgtt acctggtaat tgaataggat gcttctggct gatgtcattc ttgtgatcca
     7441 tttccttttc gccctgttcg tggtcggcag cctcccgctg atctggatag ggtcatggat
     7501 gcgctttcgc tttgcgcgaa accggtcgtt ccggtttctt cacctgatgg cgatcgtgtt
     7561 cgtcgcaagc gaatccctga ttggtatggt atgccccctg acttctctcg aagacgcgtt
     7621 gcgcggcaca ggaaacgggg gaagcttcat tcagcgctgg ttgcatcgca tcttgtatta
     7681 cgacttcccg gagtggatat tgaccgtagc ttatgtcctg tttgcattcc tggtcgcgct
     7741 aacctacatc ctggttccgc cgcgtgggag gaagcggggc tgatcgagcc gattcctcat
     7801 ctgtgaaaga ttgacccatt gcctcgcgtg agggtaaagt tgtgatagaa aaaagaggaa
     7861 taaatctctc cgcctgcttt tgccaacaaa gcttccccca caaatcgaga aaccatgtca
     7921 gctgcatcat ttctcaagag aatcgctgca caaaagcgcc gtttgggaat cggagctgga
     7981 attttggtcg gcctggttgc cctctacggg ctgctcgggt acttctggtt gccgggattt
     8041 attcggacaa aagtggaaag cgagctgtcg gcgtcattgc acaggccggt caccgtgcgg
     8101 tcaatcgaaa ttcagcccta tacgctcgaa ttcacggtgc gtgggtttcg cgtcggaggg
     8161 aagccctcgg attccgacgc aggcaatgat cttctgtcat tcgaagagct ctacgtgaat
     8221 gtgagcggtg cgtcgctgac gaaaggtgca cccgtgatca gcgctgttac gttgagaggg
     8281 cctgtcgtgc ggctcgtacg cgaaggtgaa aaccgcttca acattaccga cctgatcgag
     8341 gatttcatga agcagccccc gtccgagggc acgacgatgt tttcggtcag taatatcctg
     8401 gtggaagacg gaagtttcac cctcatcgac cagctcaagg atagccgcca ggaaatttca
     8461 gggatccgcc ttggtgtgcc gttcgttgca aacctgaaaa gtgatgagca gagctgggtt
     8521 gaaccccatt tcagtgcaaa cgtgaacggc gcgccgctga ggctcaacgg gaagcttcgc
     8581 cctttcagca ccaatcgtga agctacactg gaactcaagc taaatgacgt ggacctgacc
     8641 cgtgtggatg aatattcacc tgtccccgtt ggtatccggc tgagttcggg ctatctggac
     8701 agtaacctcg tgctgacatt cagccaggct gacagcgaag cgccaaaaat cactttaact
     8761 ggagaagcag cacttcgaaa actgggaatc gagaaccacg gtaccgagat accatacgcg
     8821 gccaagatcg cggaaatcca gatcaagcca gtcgagctca atctgaacag tgcgaaccga
     8881 tcgcacgttg cactggcttt gagcgacgcg gtgatctcgc atgagggaga agaacaacct
     8941 gtgctgagcc ttccatcgtt gaaagtgagc caatcctcca tcgatctcgc gcatcaatct
     9001 gtcacgctcg gggatataac gctcgaccga atccatgcat tcttgcggcg ggaaagtgac
     9061 gg
//
LOCUS       DASSHJ010000245         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_451520, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000245 DASSHJ010000000
VERSION     DASSHJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451520"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            787..1155
                     /locus_tag="VFI43_09525"
     CDS             787..1155
                     /locus_tag="VFI43_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062405.1"
                     /translation="MNTLSIFRKAPQNHLLLSTCLTVTKAGVIWIFFACNAAQAEKIF
                     ANQALSCESPILKAQQTESSDIGQKRARRSEIRKIRPGMKSPRGSHIKQEIDQREKLA
                     PEPFTDPAQPIHRKAPRALV"
     gene            1596..3170
                     /locus_tag="VFI43_09530"
     CDS             1596..3170
                     /locus_tag="VFI43_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062406.1"
                     /translation="MNSSFQGSQSARAQVAVALGLAAFVVFALFQWQGNKGFSLWDEG
                     YLWYGVQRVMLGEVPIRDFMSYDPGRYYWAAAFMNLWDDQGIMALRRAVAIFQVIGLF
                     VGLLLIAQNSKNQSYAYLLLSAVTLAAWMYPRHKLFDASLSMLIVGVLASLVRRPSTA
                     RYFFTGLCIGLIAIFGRNHGMYGFAGGFAALIWLSIKRTNGPPFTESLAFWAAGITAG
                     FMPIVLMALAMPDFAAAFWDSIRFLFETKGTNLHLPVPWPWRVDFDTLPVEKAVRGFL
                     VGLFFVAIVIFGLLALAWIFWQRWNQRKVSPVLCGAAFMALPYAHYAYSRADVGHLAL
                     GIFPFLIGSLAFLAARPVTAKWPFALMLFFASIWVMHPFHPGWECSTGQPCVLMEISG
                     DELRIAPNTADDVRLLRKLADEYAPEGHNFIATPFWPGAYALLERKSPMWAIYALHRR
                     NEADQRSEIARIRAANPGFILLYDFPLDGREELRFRNTHPLIHQYIIENYDLLPSSPN
                     PLYQIFKPRLRIGAAQ"
BASE COUNT          695 a          889 c          820 g          863 t
ORIGIN      
        1 tcacgccgac cttcccattt gctgcttccg ggaatacgcc ggagcacgga atctcctggg
       61 ccgataggca tactcttgct gacgactcag ggttgacaga atgcgttggg cgaggtgttg
      121 tgttgcggac aggttcgaca cgcataaaag cttgatacgt tggcagtgat gccttcgcct
      181 ggattatgat tcgcagtgag agctggaata tttgctatga ctcctttacc gattgattcc
      241 cgcacaagtt attgctcgga cctcttcctg cgccgggagc cgcgccttgg atctattctc
      301 ctggtgattt tgctcttctc cagctgcgct tccaacacgt ccccccggca attgcaaaac
      361 ggaccagggg gatcacccat ttactccctc acgacactgc atgacggtga agcagggtct
      421 cgagaacagg ccgcgaagcg gatggacatt gatgcaaaaa acatgtgtgc ctcgggaagt
      481 tatacgcgtc tctccgagga gtcggttcca atcattaacc gtcttggtga agctacattc
      541 gcccggctga tatggcaaat cgagtgcgtg caactgcctg aagaaggggg cgggccaccg
      601 aaataacact gtcctcccgg ccaagtcata ccttcattcc aggtttcagg tccgtcccgc
      661 tctttttacc tcggtcttcg ctgcgtacct ctgtcttggc tgtgcggtag cgcaccgaac
      721 cccttggggt agcaggatat tgtttcaaca ctgcaacatc gccggacagc ttgaggccga
      781 gccatcatga atactctttc gatattcaga aaagcgccgc aaaaccactt gcttctttct
      841 acctgtctga cggttacaaa agccggtgtc atctggattt tttttgcatg taatgctgct
      901 caggccgaaa agattttcgc gaaccaggcc ctgtcatgcg aatcaccgat cctcaaggcg
      961 cagcagacag aaagttctga tatcgggcag aagcgggctc gtcgaagtga aatccggaaa
     1021 atcagacccg gcatgaagag tccgcggggc tctcacatca aacaggagat cgatcagcgc
     1081 gaaaagctgg cccccgagcc cttcactgac cctgctcagc ctatccaccg gaaggcgccc
     1141 cgcgcgttgg tatgagatag aactacagta ttataatttt cggtttattc acctcatcgc
     1201 aataccagaa gaccgtttcg cgccctttgc agcactcatc ttttcccctt ttcatattct
     1261 gatttagcct gcctgctaag atagtatcct ctgcgattgc taaaatcagc cacgtgccat
     1321 tttttgttcg cttgatttgc tcccttttat ttccatcatg cgttccgacg gaggcttatc
     1381 gataggtttt attcagtctg acgcttccgg aacaataacg aacattcact attagtcaat
     1441 aatccggttc cgctgcgaac aaggcatttg ctccccttat ttaacaagct gccccgaaat
     1501 ccaatgaggc cgtttcagtt attcgttttc tgcaatacaa accccatcac aagtattcca
     1561 tcgctcatcc atctcactcc gcattgttac attccatgaa tagcagcttt caagggagtc
     1621 aatcagcgag agcgcaagta gccgtggcat tgggcctcgc cgcatttgtt gtgttcgccc
     1681 tattccagtg gcaggggaac aagggtttca gtttgtggga tgaaggctac ctttggtacg
     1741 gcgttcagcg agtcatgctg ggtgaagttc cgattcggga tttcatgtcc tacgatcctg
     1801 gccgctacta ctgggcggcg gctttcatga atctttggga tgatcagggc attatggcgc
     1861 tgcgacgggc tgtagcgatt ttccaggtaa tcgggttgtt cgtcggtctt ctgctgattg
     1921 cccaaaactc gaaaaaccag agttacgcct atctattgct ttcagcagtc acgctggctg
     1981 cctggatgta tccacgtcat aagctgtttg atgcatccct atcgatgttg atagtaggtg
     2041 tgctggcttc gttggtaaga cgtccctcca cggcgcgcta tttttttacg ggattatgca
     2101 tcgggctcat agcgattttt ggccgcaatc atggaatgta tggcttcgcc ggcgggtttg
     2161 cagcattgat atggttgagt atcaagcgca cgaacggtcc acccttcaca gagtcacttg
     2221 ccttctgggc tgcgggaatc acagccggtt ttatgcccat cgttctcatg gcgctggcga
     2281 tgcccgattt cgcggcggct ttctgggaca gcatccgctt tctttttgaa accaaaggca
     2341 ccaatcttca tttgccagtt ccctggccgt ggcgcgttga cttcgatact ttgcctgtcg
     2401 agaaagccgt ccgcggtttt cttgttggcc tgtttttcgt tgccatagtc atttttggcc
     2461 tgctagcgct agcctggata ttctggcaaa gatggaatca gagaaaggta tcgcctgtgc
     2521 tgtgcggggc ggccttcatg gctttgcctt atgcacacta tgcttattcc cgggctgatg
     2581 taggccacct cgctttaggc atctttccgt tcttgatcgg ctcccttgca tttctggctg
     2641 cccggcccgt gacagccaaa tggcccttcg cactcatgtt atttttcgcc agcatctggg
     2701 tgatgcatcc ctttcatccg ggatgggaat gcagcaccgg ccagccgtgt gtgctgatgg
     2761 agatttccgg agacgagcta agaattgcgc cgaatacggc ggatgatgtc aggttgctgc
     2821 gaaaacttgc agatgaatat gcgccagagg gacacaactt cattgcaacg cctttctggc
     2881 caggagccta cgcgttgctc gagcggaaat cgccgatgtg ggcaatatat gccctgcatc
     2941 gcagaaacga agccgatcag cggtcggaaa tcgcgcggat tcgggctgcc aaccccggtt
     3001 ttatcctgct ttatgacttc cccctcgatg ggcgcgagga gctgaggttt cgcaacacgc
     3061 atcccctcat tcatcaatac attatcgaga actacgactt gctgcccagc tcgcctaatc
     3121 ctctctacca gatattcaaa ccaagactcc ggatcggtgc agcgcaatga atccgaagaa
     3181 ccctaaagtc atccgttcgt agcattaatc tcatcctctt ccaacccgag aaaggcaaat
     3241 aggctcaagc aacggctgta ccgaggt
//
LOCUS       DASSHJ010000246         5605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_919848, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000246 DASSHJ010000000
VERSION     DASSHJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919848"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFI43_09535"
     CDS             complement(<1..210)
                     /locus_tag="VFI43_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein"
                     /protein_id="HET7062407.1"
                     /translation="MKKVIGVLLIGLLSACASQTPQPSAEVEEKSMGTQPREGTSATP
                     AEGGPTLNPLTDPKNILSKRSIYFDF"
     gene            complement(288..1586)
                     /gene="tolB"
                     /locus_tag="VFI43_09540"
     CDS             complement(288..1586)
                     /gene="tolB"
                     /locus_tag="VFI43_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET7062408.1"
                     /translation="MHIRSGKFSGYAFVLLLWLVSSPLHATLNIEIFGGGATQIPIAI
                     VPLAGEEKLKQGISPVVSADLYRSGLFKLVEPGVLAPHDPAEVVYPEWTSRGASALAI
                     GTATPLPGGLVALRVMLLDVARQSQLAIYTETVPPEQLRAAAHRIADMIYEKLTGDIG
                     VFSTRIAYVLKQGKKYSLQVADADGFNAQPVIEYTEPIISPAWSPDGTKLAYVSFENK
                     KPVVYVQTLATRARKAVANFRGSNSAPAWSPDGKKLAVVLSLMGGSQIFLINADGSGL
                     QRLTQSSGIDTEPSFSPDGRSIIFTSDRGGSPQIYRMPVSASASSTAERLTFEGSYNV
                     SPHYSPDGRSFTFVHRSGDRFNVATQDIASRQVQLLTDSRFDESPSFAPNGKMILYAT
                     EIRGRGILSAVSSDGRTRQQLSTQEGDVREPAWGPLPIRP"
     gene            complement(1643..2497)
                     /locus_tag="VFI43_09545"
     CDS             complement(1643..2497)
                     /locus_tag="VFI43_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HET7062409.1"
                     /translation="MNAAVLRNSRLPEPGRIPAAVFAVLVHVAFFAFMIFGLNWKTQP
                     PEGMMVDLWSDLPQPAPPEPVAKPVPPEPKPEPPPEPVKPVEPPPEVKPLPPRQETPP
                     PTVKPDIAIKEKPEKPKPVVKKPVEKKLLPPKDLKELVEEAERKEQKELKELKERQQK
                     ARVAAEMEQVRREQEAKERLQAQAAARSRVSNEIDEYKARILARIKRNIVMPPDLPGN
                     PVAEFDVTLLPGGDVLDVKLRKSSGFAAFDSAVERAIYLSKPLPLPPDPALFPQFRNL
                     SIKVHYLE"
     gene            complement(2757..3173)
                     /gene="tolR"
                     /locus_tag="VFI43_09550"
     CDS             complement(2757..3173)
                     /gene="tolR"
                     /locus_tag="VFI43_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HET7062410.1"
                     /translation="MSERRGRRRLMNEINVVPYIDVMLVLLVIFMITAPLINPGQIEL
                     PQIGKSLTPPVAPLEVIVKADGTLRLHDRSKPGGEQKVSRAELIEAIKRKQAQNADQP
                     VVIAGDKSVRYEEVINVMDILQQQQVKKVGLLAKPK"
     gene            complement(3189..3875)
                     /gene="tolQ"
                     /locus_tag="VFI43_09555"
     CDS             complement(3189..3875)
                     /gene="tolQ"
                     /locus_tag="VFI43_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HET7062411.1"
                     /translation="MNASVTQDMSVIHLISNASVLVQLVMVLLLLASFLSWWYIFRKL
                     FVIRRAIKQSDEFENAFWKGSDLNALFQSATSARHTAGSMERIFEAGFREFIKHKRHQ
                     GTDVSSIMDSTRRAMRATYQREMDDLESHLSFLATVGSVSPYVGLFGTVWGIMNSFRS
                     LSNISQATIAHVAPGIAEALIATAMGLFAAIPAVIAYNRYASNMDRLATRFESFMEEL
                     SNVLQRQAGG"
     gene            complement(3872..4315)
                     /gene="ybgC"
                     /locus_tag="VFI43_09560"
     CDS             complement(3872..4315)
                     /gene="ybgC"
                     /locus_tag="VFI43_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HET7062412.1"
                     /translation="MNVVQSDKFSSHSAEAFSLPVRVYYQDTDAGGVVYHSTYLDFLE
                     RARYEWLRELGFDVHSLIQVHKVIFMIRSLSIEYLKPALLDDLLHITVQPAELGRSQI
                     TISQKVTRGADILTRATVQAVCVGSDSFKPVSLPAPLRHKMGSCS"
     gene            complement(4437..5495)
                     /gene="ruvB"
                     /locus_tag="VFI43_09565"
     CDS             complement(4437..5495)
                     /gene="ruvB"
                     /locus_tag="VFI43_09565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7062413.1"
                     /translation="MIETDRLITSGPVSNREEELERALRPKHLDEYVGQEKIREQLQI
                     FIEAARRRREALDHVLLFGPPGLGKTTLAHIIAREMGVSLRQTSGPVLERPGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPALEDYQLDIMIGEGPAARSVKLDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIISRLEFYSVEELTKIVTRSASLLNANMSTDGAVEIARRSR
                     GTPRIVNRLLRRVRDFAEVKAAGHITRPVADAALLMLDVDVIGLDVMDRKLLMAVMEK
                     FGGGPVGVDNLAAAISEERGTIEDVLEPYLIQQGYMKRTPRGRMATAIAYQHFGLPLP
                     KSAPAGTSGELWGDEPSV"
BASE COUNT         1202 a         1598 c         1567 g         1238 t
ORIGIN      
        1 gaaatcaaag taaatgctgc gctttgaaag tatgttcttg ggatcagtga gcgggttaag
       61 cgtgggacca ccctcggccg gggtagcgct tgtaccttcc ctcggttgag tacccataga
      121 tttctcttcc acttccgcgg aaggctgggg ggtctggctg gcgcaagccg acaacaaacc
      181 gatcaatagc acgccaatca ctttcttcat tttcatttcc tttaaggttg aatatagagt
      241 taaaaagctg atcaaaaaga gggtgtaaag ataatgcgaa gaagatatca gggcctgatg
      301 ggtagcggcc cccatgcagg ctctctgaca tcgccctcct gagtggagag ctgctgccgg
      361 gtcctcccat cgctcgatac tgccgacaat attccccgtc cgcgtatttc ggttgcgtaa
      421 aggatcatct tcccgttagg tgcaaagctc ggggattcgt caaaccggga atcggtgaga
      481 agctgaacct gccggcttgc aatatcctga gttgccacat tgaaccggtc gccgctccgg
      541 tgaacaaagg taaaacttct accatccgga ctgtaatgcg ggcttacgtt gtagcttccc
      601 tcgaacgtga ggcgctctgc tgtcgaagac gcgctcgctg ataccggcat gcgataaatc
      661 tgggggctgc cgccacggtc ggaggtaaag atgatggaac ggccgtccgg agagaaactt
      721 ggctcggtgt cgatacctga actctgggtc agccgttgca agccgctgcc gtccgcattg
      781 atcaggaaaa tctgcgaacc gcccatcaga gataagacga ccgcgagctt cttgccgtcc
      841 ggcgaccacg ccggtgcgct gttgctcccg cgaaaattgg ccactgcctt ccgggcgcgg
      901 gtcgcaagcg tctggacgta cacgacgggt tttttgtttt cgaatgaaac atacgccagc
      961 ttcgtcccat cgggcgacca tgcgggggat atgataggct cggtatactc gatcactggc
     1021 tgggcattga agccatccgc atcggccacc tgtagcgaat acttcttacc ctgctttaaa
     1081 acgtaggcaa ttcgcgtgct gaaaacgcca atgtcgcccg tcagcttttc ataaatcatg
     1141 tcggcgatcc gatgggctgc cgcacgcaat tgctcggggg gaacggtttc agtgtaaatg
     1201 gccagctgcg attgcctggc gacgtccagc agcatgacac gcagtgcaac caggccccca
     1261 ggcaagggtg ttgcagtgcc aatcgcgaga gcgctggcgc ctcggctcgt ccactcgggg
     1321 tagacgactt cggcagggtc gtggggcgcg agaacacccg gctcaacgag cttgaacagt
     1381 ccgctgcggt acaaatcggc ggatacgacg ggactgatgc cctgtttaag cttctcctcc
     1441 ccggcaaggg gaacaatagc gatgggaatc tgcgttgcgc cgccaccaaa aatttcgatg
     1501 ttcagagtgg catgcagcgg ggagctaacc agccagagga gcaaaacaaa cgcatatccg
     1561 gaaaacttgc ccgaccgaat gtgcatggca ataataacct taatctaata aaaggatttt
     1621 aaagctttgg aattataccc agtcattcga ggtaatgcac tttaatgctc aaattcctga
     1681 attgcggaaa caatgcggga tcggggggga gcggtaacgg cttcgacaag taaatcgccc
     1741 gctcgacggc actgtcgaat gcagcgaacc cgctgctctt gcgcaacttc acgtcgagga
     1801 cgtcaccgcc cggcagcagg gtgacgtcga attcggcaac gggattgccg ggcaggtcgg
     1861 ggggcatcac gatgtttctt tttatcctcg ccaggatacg cgccttgtat tcatcgattt
     1921 cgttcgatac gcgcgaccgg gcggcggcct gagcctgcag ccgttccttg gcttcctgct
     1981 cacggcgcac ctgctccatt tctgcagcaa ccctcgcttt ttgctgcctc tccttcagtt
     2041 ccttcaattc tttctgctct tttctttccg cctcctcaac caattcctta agatccttcg
     2101 gcggcagcag tttcttctct accggctttt ttacaaccgg cttgggtttc tcgggctttt
     2161 ccttgatggc aatatcgggc ttaaccgtgg gaggcggcgt ctcctgccgg gggggcagcg
     2221 gctttacctc gggcggcggt tcgaccggct tgacaggctc gggtggaggc tcgggtttag
     2281 gctctggcgg caccggcttt gcaacgggct cgggaggcgc tggctgcgga agatcgctcc
     2341 ataaatccac catcatcccc tcaggcggct gagttttcca gttcaggccg aaaatcatga
     2401 aagcgaagaa agcgacgtgc acaagcacgg cgaacacggc tgccggaatc cggcctggct
     2461 ctgggagtct ggaattacgc agtaccgcgg cgttcattgg ttatacctgt tgaggtacat
     2521 gttccatttc tactctgcct gcccgagaac ctcagcgaac gttttcttct gccgtccaat
     2581 tgcatgccaa actgcaatcc acgctgctaa aatcgccgtt gttttccctc tcccgctcgt
     2641 aggagagggc gatcttcggg tgcgaccccc cctctctcct ggcctctcta cccacgggga
     2701 gagagaaact gacaacgttc gtggccaaaa agcggataca gggcgatcct gagctctcac
     2761 ttcggcttgg caagcaaccc cactttcttg acctgctgct gttgcagtat gtccatcaca
     2821 ttgatcactt cttcatagcg gacactttta tcccccgcaa tcaccacggg ttggtcggca
     2881 ttctgcgcct gcttgcgctt gatcgcctcg atcagctcag cccggctgac tttctgttct
     2941 cccccgggct ttgagcgatc atgcaagcgc agcgtcccgt ccgccttgac aatcacttcg
     3001 aggggcgcaa cgggaggcgt gagggatttt ccgatctgcg gcagttcgat ctgcccagga
     3061 ttgatcagcg gtgcggtaat catgaaaatg accagcagca ccagcatgac gtcgatatac
     3121 ggcacgacgt tgatctcgtt catcaggcgg cgcctgccgc gccgctcact catgacgacc
     3181 gcccatcatc atccgcctgc ctgccgctgc aagacattgg acaattcttc catgaagctc
     3241 tcaaaccggg tggccagcct gtccatgttg gaagcataac ggttataggc aataaccgcg
     3301 gggatggcag caaaaagacc catcgcggtc gcgatcagcg cctcggcaat cccaggcgca
     3361 acatgggcaa tggtggcctg gctgatattc gataagctcc ggaaagaatt catgatgccc
     3421 cacactgtgc caaacagccc gacgtaaggg ctgaccgacc ccaccgtggc gagaaatgaa
     3481 aggtgcgatt caaggtcgtc catttcccgc tggtaagtcg cccgcatcgc acggcgagtg
     3541 ctgtccatga tcgaactgac atccgtgccc tggtggcgct tgtgcttgat gaactcgcga
     3601 aatcccgctt caaaaatccg ttccatgctc cctgccgtat ggcgcgcgct tgtcgcactc
     3661 tgaaaaagcg cattcaggtc agatcctttc cagaaagcat tctcgaattc atcgctctgc
     3721 ttgattgcgc ggcgtattac gaacagcttg cgaaagatgt accaccagga taaaaacgag
     3781 gccagcagca acagtaccat gaccagttgc accagcacgc tggcgttgct gatgagatgg
     3841 atgaccgaca tatcctgggt gaccgaagcg ttcatgagca gcttcccatt ttgtggcgca
     3901 acggagccgg caagcttacc ggtttgaagc tgtccgagcc tacgcagacc gcctgaaccg
     3961 tagccctggt caggatatcc gcgccacggg tcaccttttg agaaatcgtt atctggctgc
     4021 ggcccaattc cgctggctgc acggtaatgt gcagcaggtc atccagcagc gctggcttga
     4081 ggtactcgat gctaagcgaa cgaatcatga agatcacctt gtgaacctgt atcaaagaat
     4141 gaacgtcgaa tcccagctcc cgcagccact cataacgggc tcgttcgagg aaatcgagat
     4201 aggtcgaatg gtaaacgacg ccaccggcat cggtatcctg gtagtaaaca cggacgggga
     4261 gtgaaaaagc ttctgcggag tgacttgaaa atttatcaga ttgaaccacg ttcataaatt
     4321 aaaacaaggc gagaaaaaac gccaatccaa tagttaccga aaggaagcga cctttggcag
     4381 tcccggcctt tggcagtcct ggaaaggcat agggcaagga actgctgcct gggtgttcag
     4441 actgagggtt catcccccca cagctccccg cttgttccgg cgggcgcact cttgggcaat
     4501 ggcaggccaa aatgctggta ggcaatcgct gtggccatcc tgccacgtgg agtgcgtttc
     4561 atgtagcctt gctggatcag gtaaggttcg agaacatcct cgatagtgcc gcgctcttcg
     4621 ctgatggctg cggcgagatt gtcgacgccg accggacctc caccgaattt ttccattacg
     4681 gccatcagca attttctatc catcacatcg agcccgataa cgtcgacgtc cagcatgagc
     4741 agtgcagcgt ctgcaacggg gcgggtgatg tggcctgctg ccttgacctc ggcaaagtcc
     4801 cgcacgcgcc gaagaaggcg gttgacgata cggggcgtgc ctcgcgagcg ccgcgcaatt
     4861 tcgacagcgc cgtccgtgga catgttcgcg ttgagaaggc tcgccgagcg cgtcacgatc
     4921 ttggtaagct cttccaccga ataaaattcg aggcgcgaga taataccgaa gcggtcacgc
     4981 aagggattgg tcagcattcc ggcgcgtgtg gtggcgccaa ccagggtaaa cggcggcaag
     5041 tcgagcttga ccgagcgcgc agcgggacct tcgccgatca taatgtccag ctggtagtct
     5101 tcaagcgccg ggtaaagaat ctcctctact acgggcgata gccggtgtat ttcatcgatg
     5161 aagagcacat cgttgggttc gaggttggtc agcagcgcgg cgagatcgcc cgggcgctcg
     5221 agcactggcc ccgatgtctg cctcaggctt acccccattt ccctcgcaat aatatgtgcc
     5281 aacgtggtct tgcccaggcc gggcgggcca aacaggagga catggtcgag ggcttccctg
     5341 cggcgtcgcg ccgcctcgat gaatatctgc aactgctcgc ggattttttc ctggccgacg
     5401 tactcgtcga ggtgcttggg ccgaagcgcc cgctccagct cttcctcccg gtttgaaacg
     5461 ggcccggagg tgattaatcg atcagtttct atcattcaat gattgaccct accccgattt
     5521 ctatcgaggt tttcatcttg ctttgctccg gattttcaag ggtcacacgg atgaatggga
     5581 aaaattgggg cagccttggg ctgca
//
LOCUS       DASSHJ010000247        11977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_879327, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000247 DASSHJ010000000
VERSION     DASSHJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879327"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VFI43_09570"
     CDS             complement(<1..164)
                     /locus_tag="VFI43_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177907.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7062414.1"
                     /translation="MVKAKSTYSCSGCGGQTLKWQGQCPHCQAWNTLVEVVAEKAASR
                     FIPISESGRV"
     gene            complement(157..729)
                     /locus_tag="VFI43_09575"
     CDS             complement(157..729)
                     /locus_tag="VFI43_09575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET7062415.1"
                     /translation="MTRIIGISGSLRSGSFNTALLRASAQLVPEGTVLEVETLKGIPL
                     YDGDLEAAEGVPATVTALKDRIVNADGLLLVTPEYNNSLPGVFKNGIDWLSRPPGDIP
                     RVFGGRPVAVMGASPGGFGTILSQNAWLPVLRTLGTRPWFGGRMMVSRANNVFDEKGE
                     IVDQHLRAQLQQFINGFTDFVKASRAGQDG"
     gene            1128..1700
                     /locus_tag="VFI43_09580"
     CDS             1128..1700
                     /locus_tag="VFI43_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET7062416.1"
                     /translation="MGKNRLEAFSDGVIAIIITIMVLEMKVPHGDDLSALDPLAPVFI
                     SYILSFVYVGIYWTNHHHLLHAVQKISGPILWANLHLLFWLSLFPFVTGWMGENNFST
                     WPVALYGTVLLMAAIAYSILELALIKLQGKDSLLAVAVGRDFKAKISLIMYAAAIPFA
                     FLSRWLAIGLYVIVAVMWVIPDRRIEKELK"
     gene            1758..2651
                     /locus_tag="VFI43_09585"
     CDS             1758..2651
                     /locus_tag="VFI43_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7062417.1"
                     /translation="MEVTKAEKSVKKLRRIQRSTEHHWVGNGFPVRTLLGYSTLGTTI
                     SPFLLLDYAGPMEFPPTEKRLGVGGHPHRGFETVTIVYSGEVEHRDSSGGGGKIGPGD
                     VQWMTAASGLVHEEFHGQDFARRGGMFEMVQLWVNLPAKDKMSPPQYQAISSNQIPTV
                     NLPDNQGSVRVIAGQFNGAVGPAHTFTPVHVWDMRIDSEQQICLPLPDGYTTILVVLK
                     GSMLFGSDSIGSGEAGIFDRAGDHICIDKAQNVTALLLCGEPIDEPIVGSGPFVMNTE
                     EEIRQAMVDYQSGRMGVVRTA"
     gene            2688..4334
                     /locus_tag="VFI43_09590"
     CDS             2688..4334
                     /locus_tag="VFI43_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7062418.1"
                     /translation="MGNPADTDIPDARASGAWAPLGLPVFRIFWLASLGSNIGTWANS
                     TTSGWVMTGLSPSPVMVSLVQAAAVFPTVLFALPAGAFTDIVNRRYYLLATQVWMTLA
                     AGALAFLAASGQLDSWNLLILTFALGFGGAMSTPALNVTAPELVPRNLLPQAVALGSL
                     SMNISRAIGPAIAGLLLAQLGAWAAYGMNAVSFVGMVVMLWYWKHQPDKRSLPPERFF
                     QALRAGVRYAQMASPFRAVLIRSTAFIIFATSIWALLPLIARNELGGGPSTYGFLLTC
                     VGIGAVGIIVILPRLHLHLSRDGLVLTGSIVFALTTIAVATIHNETVLYGIMIVCGAA
                     WVSVLSSLQVAAQISVPAWVRGRALALYMMIFSAGMTLGSVIWGWIASHAGTPAALLT
                     SAVAGTLAAIAVRKFSLGSLEAPDLSPSNHWPHPPVVEEPGGERGPVLVTVEYLVAVD
                     KRQAFLQAIRPLGEIRRRDGAFGWGVFEDIAAPGRYLEFFQHDSWLDHLRQHERVTRE
                     DQRVQEQVNSFHTGSEAPKVSHFVAGSPPASTDSSTVSAP"
     gene            4528..5421
                     /locus_tag="VFI43_09595"
     CDS             4528..5421
                     /locus_tag="VFI43_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7062419.1"
                     /translation="MPAIKSILVAIDFSQFSQRASKRAGMLGALLNSDTIDLLTVKEA
                     GLPDTLALVLKSTRVAVEALLVEKAVRELGLISAHLEDNYGIRCTKTVRFGRPEKEIA
                     ARADELPADLTVIGEHGSNFFTSLFLGNTVDKLARIIKTPLLVVKKEAATSYQRVLVP
                     VDFSENSKSAARMALKVAPDAKLDFLHVFDVVLEEQMQSADIANNFLHDYHIEAEENA
                     RRDLNQFIADIPESHPISREVAFGHPGHVIYDHAESTRPDLIVLGKHGKSGLEDLLLG
                     SVSRHVLEQSSCDVLMVMGRV"
     gene            5677..8709
                     /locus_tag="VFI43_09600"
     CDS             5677..8709
                     /locus_tag="VFI43_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HET7062420.1"
                     /translation="MSLIWIVILPFAGSLCAAFLPSHARNAAAWLAETVAVVCIVLIF
                     RLYWQIAEGEVVREAFQWAPALGLYVTFRMDGFAWLFATLVTLMGALVSLYARYYMAA
                     QDPVPRFFSFFLAFMGAMLGVVLSGNLIQLVVFWELTSLMSFMLIAYWYHRADARRGA
                     RMALTVTSAGGLCLLAGVLILGRIVGSYDLDAVLDSGDRIREHPWYLAVLVLIALGAL
                     TKSAQFPFHFWLPHAMAAPTPVSVYLHSATMVKAGVFLLVRLWPVLAGTDEWFWLIGT
                     AGLCSLVLGAYAAMFQRDMKGVLAYSTISHLGLITLLLGLNSSLAMIAAIFHILNHAT
                     FKASLFMAAGMVDHETGTRDLSRLSGLYRAMPITAMLAAVGAASMAGVPLFNGFLSKE
                     MFFAETVFVSGNLVTRLGLPLMAVLWGTFSVAYSLRFILQVFFGPPARDLPRHPHEPP
                     MWMLLPSALLVTTCLVVGVFPAQTVQPYLDVAARSVLGAEMPFHSLKVWHGFNLPLVM
                     SLVAIIGGILLYRALGKYQQGASVVPTVVPVINRFDGKRIFESFLGRLDALARLLMRW
                     LSTERLQVQVLLLIGAVFASAAIPLSNGGLPEGDKLMAAAEPAFALMWLVGGVCAIGA
                     ATQAKYHRLAALSLSGGAGLATCMTFVWFSAPDLALTQLIVEVVTLVLLLLGLRWLPP
                     RYKWLDDKTIPFQARIRARSRRLRDLVLAIVAGAGMAALAFAVLTRQAGEGISPFFIE
                     NSLALAGGSNVVNVILVDFRGFDTMGEITVLGVVALTVFALLRRFRPASESVGLPIQQ
                     QEQDVQAVAPPPAPQGLLPQGPMMLPAVIVQLLLPVSWMVAAFFLLRGHNEPGGGFVA
                     GLVVTTAVILQYIVGGTAWAESRTRIYPQYWVATGLLCAAGAGLLAWLAARPFLSALV
                     LHADFPIIGEMHLSTVLLFDVGVFLLVVGASMLILVALAHQSRRGHRKPAGAQVEVGE
                     EVFPVALAVAPGAPSTHAAQTGLASPSISAMGHVDKES"
     gene            8719..9066
                     /locus_tag="VFI43_09605"
     CDS             8719..9066
                     /locus_tag="VFI43_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HET7062421.1"
                     /translation="MEAIYAMAIGLLTGSGVWLVLRPRTFQVIMGLSLLSYAVNLFIF
                     GMGRLMIEGAPIIETSSLADPSLYDDPIPQALVLTAIVIGFATTALFLVVLLASRGLT
                     GNDHVDGDGPDHE"
     gene            9035..10681
                     /locus_tag="VFI43_09610"
     CDS             9035..10681
                     /locus_tag="VFI43_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HET7062422.1"
                     /translation="MSTGTGRIMSSWMQHLPVLPVIIPLVAGAGMLLFAEGRRTERTI
                     IALVATLLNLTVAGVLAYAADGWMPDIWPGGIAVYQLGGWQAPFGIVLVLDRLSAVMI
                     VLSAVLALTSLVYALARWKKMGPHFQSLFQFLIMGINGAFLTGDLFNLFVFVEVLLAA
                     SYGLLLHGLGQPRVKAGLHYIAINLAGSFVFLIGVSLIYGVTGTLNMADVAERAARLI
                     EGDRILFQTGAAILGIAFLLKAGAWPLNFWLPAAYAAAAPPVAAVFSILTKVGIYAML
                     RLGSLLSTSSASGAPVPFNDGWLFAAGLVTLVLGTAGMLTAIQPQRLVAYCVIVSAGT
                     LLAALGLGGAAMKGAALFYLASSVLATGAFFMLSEMIVRTQHSPAAPELPSKIESHEP
                     DFSDDEVGIVIPAAMAFLGMSFLACALLVTGLPPLSGFIGKFLILSAALDLATLETLP
                     ASVWVLWTAVLSSSLVGIIALSRMGMRLFWSSDEIVTPRLQLIEAAPVAVLLLLSVVL
                     ATETGPAMDYLSLAADSLSSPHTYIDAVLGNRSRVGVEGD"
     gene            10685..11173
                     /locus_tag="VFI43_09615"
     CDS             10685..11173
                     /locus_tag="VFI43_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HET7062423.1"
                     /translation="MKSWPSLTLFTAILFGIWLLLNNSLSAAQIVLGLVLTIVIAALT
                     AAIRPLTARPHHLLTAVRLFLVVLWDIVRSNFAVARVILGTEKRRSNSGFMSIPIDMR
                     DQYGLAMLACIITATPGTLWAGLSQDGCALTIHVLDLQDEETWVNTIKQRYERPLMEI
                     FE"
     gene            11170..11451
                     /locus_tag="VFI43_09620"
     CDS             11170..11451
                     /locus_tag="VFI43_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HET7062424.1"
                     /translation="MRELLPWAISFSLMTVALAMVFAAIRLLRGPAAEDRVLALDTLY
                     ANSMIMVLTLGIQQGSRIYFDIALLMALFGFVGSAAMAKFLLRGEVIEP"
     gene            11448..11804
                     /gene="mnhG"
                     /locus_tag="VFI43_09625"
     CDS             11448..11804
                     /gene="mnhG"
                     /locus_tag="VFI43_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HET7062425.1"
                     /translation="MTIETLPLWADLLASFLLITGSVLTLIGSSGLLRLPNLFARIHA
                     PTLGNTLGLGCVLLASMLVASMQTDRLVLQEVLLTLFAVATSPVTAILLMRAGIYRRR
                     TDTNKKKRDHQDTAAT"
BASE COUNT         2316 a         3288 c         3392 g         2981 t
ORIGIN      
        1 tgtacgcgcc cgctttccga aatcggaatg aagcgggatg ccgctttttc tgcgacaacc
       61 tcaaccagcg tattccaggc ttgacagtgg gggcactgac cctgccattt cagtgtctgc
      121 ccgccgcatc cggagcagga ataggtggat ttggccttaa ccatcctgcc ctgctctgct
      181 ggcctttacg aaatcggtga agccattgat aaactgctgg agttgcgcac gaaggtgctg
      241 atccacgatt tctcccttct catcaaatac attgtttgcc cgggatacca tcattcgtcc
      301 gccaaaccag ggacgggtac ccaacgttcg caaaacaggc agccaggcat tttgagagag
      361 gatggtacca aaaccgccgg gagatgcgcc catcactgca accgggcggc cgccaaatac
      421 gcggggaatg tcgcctggcg gccgagatag ccagtcaata ccgtttttga atacgccggg
      481 taaggagttg ttgtactcgg gcgtgaccag caataatcca tcggcattca ctatacgatc
      541 tttcagtgct gtcaccgtcg ccggaacacc ttccgcggct tccaggtccc cgtcatagag
      601 cggaatgcct ttgagcgttt caacctccag caccgttcct tccggcacaa gctgcgctga
      661 tgcacgcagc agcgccgtat taaacgagcc gctccggagg ctgcctgaaa tccctatgat
      721 tcttgtcata atgccttctc ctttcgttgt atgttccatc cacctatagc tgattcaacc
      781 ccatctccca ataggggatc ttctctgagt accgcaagct tgatgaaaca tattcccctt
      841 acctcgcgat cattccattg ccagcaatgc tgcttccagg gtcaataatg cattggccaa
      901 tggattccga aaagctcatg cgctagcccg ctgtatgagc gctcactgct tccaatcgaa
      961 tactggtctg aaggaaggcg gtttcgaggc actggaaagg cgtaagttac aggctataca
     1021 gtctatattg aaacatgtgc cgcgatgaca acagatcaca ttgcagactt tcatcctccg
     1081 ccccgaactg gatttatact ggcgccagct tcccaacgta gggttttatg ggaaaaaatc
     1141 ggctggaagc gttcagcgat ggagtgattg caatcatcat caccatcatg gtacttgaaa
     1201 tgaaagtgcc gcatggagat gatcttagcg cactcgaccc cctagccccg gtttttatca
     1261 gctacatcct cagcttcgtc tacgtcggta tttactggac caaccaccac cacttgctcc
     1321 atgccgtaca aaaaataagc gggcccattt tatgggcaaa tctccatctg ctgttctggc
     1381 tttccctttt cccctttgtt accggctgga tgggcgagaa caatttttcc acttggccgg
     1441 tcgcgctgta tggaaccgtg ctgttgatgg ccgcaatcgc ctattcgatc ctggagcttg
     1501 cactcatcaa gcttcaggga aaggattcgt tactggctgt ggcggtcggc cgcgatttta
     1561 aagcaaagat atccctcatc atgtatgcgg cagctatacc cttcgcattc cttagccgat
     1621 ggcttgccat cggtctttat gtaatagtgg cagtcatgtg ggtaattcct gatcgtcgca
     1681 tcgaaaaaga gcttaaataa acttgaaatt accatcttgc acagcctttt accttttaaa
     1741 ttggagagga gacccttatg gaggtaacga aagccgaaaa atcagtcaaa aagctccgac
     1801 gtattcagcg cagcaccgaa catcactggg ttggaaacgg ttttcctgtg cgtacgctcc
     1861 ttggctattc aacgcttgga acgacgatca gccccttcct gttgctcgat tatgccggcc
     1921 cgatggaatt ccctcccacc gaaaagcgcc tgggtgttgg cggccaccca catcgcggat
     1981 tcgagacggt gaccattgtc tatagcggcg aagtcgagca tcgggacagt tccggcggcg
     2041 gggggaaaat tggcccgggc gatgtgcaat ggatgaccgc ggcctccggc ctcgtacatg
     2101 aggaatttca tggccaggat tttgctcggc gcggcgggat gtttgaaatg gtgcagttgt
     2161 gggtcaatct gcctgcaaag gacaagatgt caccaccgca gtaccaggca atttcaagca
     2221 atcagattcc cactgtgaat ctgcctgata accagggaag cgtacgtgtc atcgcgggtc
     2281 aattcaatgg ggctgtcggc ccggcccata cgttcacacc tgttcatgtc tgggacatgc
     2341 gcattgatag cgagcaacag atctgccttc ccctgcccga cggctacacc accatacttg
     2401 tggtgcttaa aggctcaatg ctttttggaa gcgacagcat tggctcgggg gaggccggga
     2461 tatttgaccg ggcaggcgac catatctgca ttgacaaggc gcagaacgtc acggcactgc
     2521 tcctctgcgg cgaaccgatc gatgaaccga tcgtcggtag cggacctttt gtcatgaata
     2581 ccgaggagga gatccgccag gcaatggtgg attaccagag cggcaggatg ggagttgttc
     2641 gaacggcatg attctccgat gccttctgat gtccagccaa tccgcctgtg ggcaaccctg
     2701 ctgacacaga cataccggac gctcgggctt ccggcgcctg ggctccgctc ggcttgcccg
     2761 tgttccggat tttctggctt gcatcgctag gttccaacat agggacgtgg gcgaactcca
     2821 caacatccgg ctgggtgatg acaggactat caccctcacc tgtcatggtc tcgctggtgc
     2881 aggcagcggc cgtatttccg acggtcctgt ttgctctacc ggccggcgcg ttcacggaca
     2941 ttgtcaatcg ccgctactac ctgctggcca cccaggtatg gatgacgcta gcggccggcg
     3001 cgcttgcttt tctcgctgct tccggccaac tggattcctg gaatctgttg atactgactt
     3061 ttgcgctagg ctttggcggc gccatgtcga cgcccgcgct caacgtgacg gcacctgaac
     3121 tcgttcccag gaatctgctg ccccaggcag ttgcgttggg ctctctctcg atgaatatct
     3181 cgcgcgcaat aggacctgct atcgccggtc tcctgctcgc acaacttggg gcgtgggctg
     3241 catacggcat gaacgcggtt tcgtttgtgg gaatggtcgt gatgctttgg tattggaaac
     3301 accaacccga caagcgctcc ctaccgcccg aacgcttttt tcaggcgctg cgcgcaggtg
     3361 tgcgatatgc gcagatggca tctcctttcc gcgcagtgct gatccgctca actgcattca
     3421 ttatcttcgc gacctccatc tgggcgctgc tcccgctgat tgctcgcaac gagctcggcg
     3481 gcggtccgtc gacctatggc tttcttttga cctgtgtcgg cataggagcg gtgggaatta
     3541 ttgtcattct gccccggttg catctgcatc tctcgcgtga cggcctggtg ctcacaggaa
     3601 gcatcgtctt tgcgctgacc accatcgctg tggcgacgat ccataatgaa acggtgttgt
     3661 acggcatcat gatcgtttgc ggcgccgcct gggtcagtgt tctatcgtca ctgcaagtcg
     3721 cggcgcagat atcggttccc gcgtgggtca ggggtcgcgc cttggccctt tacatgatga
     3781 tattttccgc gggcatgacg cttggcagtg tcatctgggg ttggattgcg tcacatgcag
     3841 gtactcctgc cgcccttctg acctcggctg tcgccgggac actcgcggca attgctgtcc
     3901 gaaagttcag cctcggcagc ctcgaagcgc ctgatttgtc tccatccaat cattggcctc
     3961 atccgcctgt cgtggaggaa ccaggtggcg agcgtggccc ggtgctggtc acggttgaat
     4021 acctggttgc cgtggataag cgccaggctt tcctgcaggc gattcgaccg ctcggggaaa
     4081 tccggcggcg ggacggtgcg ttcgggtggg gtgtttttga agatattgct gcgccggggc
     4141 gctatctgga gttcttccag cacgactcat ggctcgatca tctacgccag catgaacgag
     4201 tgacccgcga agaccagcgg gttcaggaac aagtaaacag ctttcatacc gggagcgaag
     4261 cgccaaaagt ttcgcatttc gtcgcagggt cgcccccggc atctacggac agctccacag
     4321 tgagcgcccc atgaccaagt tcactcgctc ggcataaggt ctgccgtgtt acgtgtttcc
     4381 ttgcctgcgt gtttccttgc ttcccgtttc gaggcatgct gcttacacta tcatggcact
     4441 atcgttttta tcagaaggag aaaatcatga aactacgtca cgcccatgag cggcggaaat
     4501 cgttgaccac gtctgcggga tagacgtatg ccggccatta aaagcattct ggtagcaata
     4561 gacttttccc aattctccca gcgggcgagt aagcgagcag ggatgctggg cgctctcctc
     4621 aactccgaca ccattgattt gttgacagtg aaagaagcag gattgccgga cacgcttgct
     4681 cttgttctca agagtacgcg tgtggcagtc gaagcgctgc ttgtcgagaa agctgtgcgt
     4741 gagctagggc taatctcggc gcatctggag gataactacg ggatccgctg taccaaaacg
     4801 gtcaggtttg ggcgacctga aaaagagatt gctgcaagag cagatgaatt gcctgcggat
     4861 ttgacagtta ttggcgagca cggaagcaat ttttttactt ctctctttct cggcaacacg
     4921 gtcgataaat tggcgcggat aattaaaaca cctttgctgg tagtaaagaa ggaagcggca
     4981 acatcctacc aacgcgtact ggttccggtt gatttttccg agaattcgaa aagcgcagcg
     5041 cggatggccc tgaaggttgc gcctgatgca aagctcgatt ttttgcacgt gtttgatgtc
     5101 gtgctggagg aacaaatgca gtcggcagac attgccaata acttcctcca tgactaccat
     5161 atcgaagccg aagaaaacgc tcgccgcgat ttaaatcagt ttattgcaga cattccggaa
     5221 agtcacccaa tttcccgcga ggtggctttt ggccatcctg gccatgtgat atacgatcat
     5281 gcggaatcaa ccaggccaga tttgatcgtc ctgggaaaac atggaaaatc tggtcttgag
     5341 gacctcctgc tggggagcgt atcgcggcat gttcttgagc aatcttcctg cgatgtgctc
     5401 atggttatgg ggcgagtctg atgggtcagt caagccaaac agaaagccct gtaatgtcaa
     5461 acaaggcagc gctggcctag ttaaagtagc ttaatgtagc ttgctgccgg ccatatgctg
     5521 gcgaggaccc aactccccgc ctcaatttac atcccagggt ataccccggc ggttcacgat
     5581 gccggcggac ccactcgttt gtccgttcgt gaggaaatct caagggaatc ccggggaaat
     5641 ttcaagggaa atctcaagga aatatatcaa ggcctcatgt cgctaatctg gatcgttatt
     5701 ctgccatttg ctgggagcct ttgcgccgca ttcctgccgt cccatgcacg caatgctgcc
     5761 gcatggctgg cggaaaccgt ggcggttgtg tgtatcgtct tgatctttcg gctttactgg
     5821 cagatagccg aaggggaggt ggtgcgggaa gcgttccaat gggctccagc cctcggcctg
     5881 tacgtcacgt ttcgcatgga tggctttgcg tggctgttcg caacgctggt gaccctgatg
     5941 ggcgcccttg tttcccttta cgctcgctat tacatggctg cacaggatcc cgtaccgcgg
     6001 tttttctctt tctttctagc ctttatgggc gccatgctcg gcgttgtgct atcgggcaac
     6061 ctgattcagc tggtagtgtt ctgggaattg accagcctga tgtcgttcat gcttatagca
     6121 tattggtatc accgggcgga tgcacgacgc ggtgctcgaa tggcattgac tgtgacttcg
     6181 gctggcgggc tttgcctgct cgccggcgtg ttgatcctgg gacgcatcgt aggcagttac
     6241 gacctggatg cggtactgga ttcgggtgac cggattcgcg aacacccgtg gtatctggcc
     6301 gtgctcgttc tcattgccct aggcgcgctt accaaaagcg cgcaatttcc gttccatttc
     6361 tggttaccgc acgcgatggc tgcgcctaca ccggtgtctg tttatctgca ctcggcaact
     6421 atggtcaaag ctggcgtatt tctgctggtg cggctctggc ccgtgcttgc cggcaccgat
     6481 gaatggttct ggcttattgg cacagccggt ctttgttcgc tggtactggg tgcatatgcc
     6541 gcgatgttcc agcgggacat gaaaggcgtg cttgcatatt ccaccataag ccatctggga
     6601 ttgataacgc tgctgcttgg gctgaatagt tctttggcga tgatcgcagc catttttcac
     6661 atcctgaacc atgcgacctt caaagcctca ctattcatgg ccgcgggcat ggtagaccac
     6721 gagacaggca cacgggactt gtcgcggcta agcgggttat atcgcgccat gcctattacg
     6781 gccatgctcg ccgcggttgg agcggcttca atggctggtg taccgctgtt caacggcttc
     6841 ctttcgaaag agatgttttt cgctgaaaca gtattcgtca gcggcaatct tgtcacgcgc
     6901 ctcggcttgc ccctcatggc tgtcctctgg ggaacgttca gcgttgccta ttcattgcga
     6961 ttcatcctgc aggtattttt cgggccacct gcacgggatc tgccgagaca tccgcacgag
     7021 ccgccaatgt ggatgctttt gcccagtgcg ctgctggtga caacatgcct ggtggtaggc
     7081 gtgttccccg cgcaaaccgt ccagccttat ctggatgtgg cagcgcgttc ggtgctgggg
     7141 gcggaaatgc ctttccacag tcttaaggta tggcatggct ttaaccttcc tctagtcatg
     7201 agcctggtcg ccatcatcgg aggaatcctg ttgtatcgcg ccctgggaaa ataccagcag
     7261 ggcgccagcg tggtccccac cgtggtccct gtgatcaacc gctttgacgg caagcgtatc
     7321 ttcgaatctt ttcttggaag gctcgacgcc cttgcaaggc tcctgatgcg atggttgtcc
     7381 acggaacgcc tgcaagtcca ggtgctgctt ctgatcggtg cagtattcgc cagcgcagcg
     7441 atccccttgt cgaatggagg actgccagag ggcgataagt taatggcggc agccgaacct
     7501 gctttcgcat tgatgtggct tgtcggtgga gtatgtgcga ttggcgccgc tacccaggca
     7561 aagtatcatc gtcttgctgc gctgagcttg tcagggggtg caggactcgc cacctgcatg
     7621 acgtttgtct ggttttccgc acccgatctt gccctgacgc aactcatcgt agaagtcgtc
     7681 accctcgtct tgctccttct gggcctgcgt tggctacccc cgcgctataa gtggctcgac
     7741 gataaaacga tcccattcca agcccgtatt cgtgcacgaa gcaggcggtt acgcgatctt
     7801 gtcctggcaa ttgtcgccgg tgccggcatg gctgcattgg cctttgccgt gctgacccgg
     7861 caggcggggg aaggcatctc gccatttttc atcgagaatt cactggcgct ggcaggcgga
     7921 tcaaatgtgg tcaatgtcat tcttgtcgat ttccgcggtt tcgatacgat gggcgagatt
     7981 acggtgctgg gcgtcgtggc gctaacggta ttcgcgctgt tgcgccggtt tcgccccgcg
     8041 agtgaaagcg tggggttgcc tattcagcag caggagcagg atgtgcaagc cgtcgccccg
     8101 ccgccagctc cgcaaggact tctgccccaa ggccccatga tgctgccggc agtgatcgtg
     8161 caattactgt tgccggtttc gtggatggtg gcggcgtttt ttttgctgcg cgggcacaat
     8221 gaaccgggtg gcggcttcgt tgccgggctg gttgtcacaa cggcggttat cctgcagtac
     8281 atcgtgggtg gcacagcctg ggcagagtcc cgcacgcgca tttatccgca gtactgggtc
     8341 gcaaccgggc tgttgtgcgc agcgggcgct ggtctcctgg cctggctggc ggcgcgtccg
     8401 tttctatcag ccctggtatt gcatgccgac tttccaataa tcggtgagat gcacctttcc
     8461 actgtactgc tgtttgacgt gggtgtcttt cttctggtgg ttggggcgag catgttgatc
     8521 ctggtggcgc tggcccatca gtcccgtcgg ggacatcgca aacccgccgg cgcgcaagtc
     8581 gaggtggggg aggaggtttt cccggttgcg ctcgcagttg cacctggggc gccgtctacg
     8641 catgcagctc agactggact tgcctcgccc tccatctccg caatggggca cgtcgataag
     8701 gagagttaag gaaagttaat ggaagctatc tatgctatgg caatcggttt attgaccgga
     8761 tcaggcgtct ggctggtgtt gcgtccccgc accttccagg taatcatggg cctctctctg
     8821 ctttcctatg ccgtgaattt attcattttc gggatgggac ggctgatgat agaaggcgcg
     8881 cctattatcg aaacctcaag cttggccgat ccgtcgcttt acgacgatcc gataccacag
     8941 gcgctggtac tgaccgccat cgtgatcggc ttcgctacca ccgccctgtt cctggtcgtg
     9001 ctgctggctt cccgcggctt gacaggtaac gatcatgtcg acggggacgg gccggatcat
     9061 gagtagctgg atgcagcatc ttccggtact tccggtgatc atcccgctgg tagcgggcgc
     9121 tggaatgtta ttgtttgccg aaggccgacg cacggaacgg accattattg cccttgtcgc
     9181 taccctgttg aatttaacgg tcgcaggcgt tctggcttat gcggcagacg ggtggatgcc
     9241 ggacatctgg cctggaggga ttgcggtcta ccaactgggc gggtggcagg cgccgttcgg
     9301 cattgtgctg gtgctggacc ggctctccgc agtgatgata gtactgagtg cggtgttggc
     9361 gttgacgtcc ctcgtatacg cgctcgcgcg gtggaagaag atggggccgc attttcagtc
     9421 gctgtttcag ttcctgatca tggggataaa cggcgcgttc ctgactggag atctattcaa
     9481 tctgtttgta ttcgtggaag tactgctggc cgcctcctat ggtctgcttc tgcatggcct
     9541 gggccagcct cgggtcaagg ccggactaca ctatatcgcc atcaatctcg caggttcttt
     9601 tgtctttctg atcggtgttt cattgattta cggggtgact ggaacactga atatggcgga
     9661 tgtagccgaa cgcgcggcaa gactcattga aggcgaccgc attctgttcc agaccggcgc
     9721 cgcaattctt ggcatcgcgt tcctgctgaa agccggggcg tggccattga acttctggct
     9781 tcccgcggcc tatgcagccg ccgcgccgcc agtcgccgca gtattttcga tattgaccaa
     9841 ggtgggaatt tacgcaatgc tgcggctggg atcgttgcta tccacctcat ccgcttccgg
     9901 cgcgcctgtg ccatttaatg acggatggct attcgccgct ggcctcgtaa cgcttgttct
     9961 gggcacggcg ggaatgctca ctgcgattca gccacagcgg ctggtagcgt attgcgtaat
    10021 cgtgtcggca ggcacgctgc tcgcagcgct cggattggga ggcgccgcaa tgaagggcgc
    10081 cgccttgttc tatctggcaa gttcagtcct ggccactggt gcttttttca tgttgagcga
    10141 gatgattgta cgtacacaac attcccctgc ggcgcctgag ctgccaagta agatagaatc
    10201 gcacgaaccg gacttttcgg atgacgaggt ggggattgtc ataccagcgg cgatggcatt
    10261 tctcggcatg agctttttag catgcgcact gctggtgaca ggcttgccac cgctctccgg
    10321 ctttatcggc aagtttctga ttctctcagc cgcgctcgac ctggccaccc ttgagacgct
    10381 gccggcatcg gtgtgggtgc tctggacagc ggtgctctcg tcgagcctgg tcggcatcat
    10441 tgcactatcg agaatgggca tgcggctatt ctggagttca gacgagatcg taacaccgag
    10501 actgcaattg attgaagccg ctccagtcgc agtgctgttg ctgctaagtg tagtgctggc
    10561 gacggaaacg ggccctgcga tggattatct gagccttgca gctgattccc tttcttcgcc
    10621 tcacacttat atcgacgcgg tgctgggcaa tcgttcaaga gttggcgtcg agggcgattg
    10681 agccatgaaa agctggcctt ccctgacgct atttaccgcc atcctgtttg gcatatggct
    10741 tctgctcaat aatagcctga gcgcagccca gatcgtgctg ggcctggttt tgacgattgt
    10801 tatcgccgca ttaacggctg cgataaggcc gttgaccgcg cgaccccacc atctgttaac
    10861 ggcagtaaga ctttttctcg tcgtattgtg ggatatcgtc cgttccaact tcgctgtggc
    10921 gcgggttatc ctcggcactg aaaaaagacg ctccaattcg ggtttcatgt caattcccat
    10981 tgatatgcgt gatcaatatg gactggcgat gctcgcctgt ataatcacgg caacgcctgg
    11041 tactttatgg gcggggctct ctcaggatgg gtgtgccttg acgattcacg tgctggattt
    11101 gcaggatgag gaaacctggg tgaacacgat caagcaacgt tacgaacggc cactgatgga
    11161 gattttcgaa tgagagaact gctgccgtgg gcgatttctt tttcgctcat gactgtcgca
    11221 ctcgcaatgg tctttgcagc aatccgcttg ctgcgcgggc ctgccgcgga ggaccgcgtg
    11281 ctggcgctcg acaccctgta tgccaacagt atgatcatgg tgctgaccct cggcatccag
    11341 cagggctccc gcatttattt cgatatcgcc ctgctaatgg cgctgttcgg tttcgtggga
    11401 tcagcggcga tggcaaagtt cctgctgcgg ggagaggtga tcgagccatg acaatcgaga
    11461 ctctcccgtt gtgggcggac ttgcttgctt cgtttttatt gatcactggc agcgtgctga
    11521 cactgatcgg atcgtctggt ctgctaaggc tgcctaacct gtttgcacgc atacatgcac
    11581 ctaccctggg caataccctt ggtctcggtt gcgtgttgct tgcgtcaatg ctggtcgcct
    11641 cgatgcagac cgatcggctg gtattacagg aagtgttgtt aacgctgttt gcggtggcaa
    11701 cgtcgcccgt gacagcgatt ctgctcatgc gtgcggggat ataccgcaga cgtacggata
    11761 ccaacaaaaa aaagcgcgat caccaagata ctgccgccac atgaattttc ggacggaatt
    11821 aatctccccc aagtactgga aatcgtcctc gtcaaatttc gcattctgac gtacacgctg
    11881 acctgacaaa agcagctaac actttttctc ctcggaacgt cgatctccca actggccgga
    11941 aagctacaaa ggcttgagac cagtcactaa actatag
//
LOCUS       DASSHJ010000248         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_266158, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000248 DASSHJ010000000
VERSION     DASSHJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266158"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1224)
                     /locus_tag="VFI43_09630"
     CDS             complement(7..1224)
                     /locus_tag="VFI43_09630"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7062426.1"
                     /translation="MSINPGAMLGILGGGQLGRMFTMAAQSMGYRVTVLDPDDNGPTS
                     SIADRHLAADYLDPSALRELGSTCAAVTTEFENVPAEALRILATYCNVSPAADSVAIA
                     QNRIREKQFLSSNGFQVAPFRVIDCEWNSNGASNGLREAPLGEFENLYGDLFPGILKV
                     SRFGYDGKGQALVNDVEELKNAFDNMNREPCVLEKLMPLKTEISVIVARGFNGAASAF
                     TVPENRHRKGILDVSIAPARLPSSIVGTAREAAMKIAERLDYNGVLCAEFFVLEDGRL
                     LVNEIAPRPHNSGHYTIDACITSQFEQQVRVLCGMPLGSTAMHSAAVMVNLLGDLWSR
                     GEPEWEQILRHPGVKLHLYGKHSARPGRKMGHYTVLDDDVEAALQLALEIKQSLEIEQ
                     CLEGSTVEKQGSE"
     gene            complement(1221..1712)
                     /gene="purE"
                     /locus_tag="VFI43_09635"
     CDS             complement(1221..1712)
                     /gene="purE"
                     /locus_tag="VFI43_09635"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7062427.1"
                     /translation="MGKPVVGVVMGSNSDWEVMKHSVEVLKDFGVPWEARVVSAHRTP
                     DRMFEYAGSAATRGLKCIIAGAGGAAHLPGMIAAKTILPVLGVPVTSRQLQGLDSLLS
                     IVQMPKGVPVATFAIGEAGASNAALFAVALLAISDAGLARRLDEFRAAQQATVEAMVL
                     PES"
     gene            complement(2160..2603)
                     /locus_tag="VFI43_09640"
     CDS             complement(2160..2603)
                     /locus_tag="VFI43_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HET7062428.1"
                     /translation="MLHPRAANLSRRTLYIFEGAGLIVIAIATIFAGYQETLSMIRTA
                     RVTLADLLLMFLYLEILTMVGLYFESGKLPVRFPLYIAMVAMARYVIVDIKEMDNVRL
                     LGVSASIVLIAVAVLVIRYGHVRYPYIEDMEDIGESTDEHTPLRK"
     gene            complement(2714..3493)
                     /locus_tag="VFI43_09645"
     CDS             complement(2714..3493)
                     /locus_tag="VFI43_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7062429.1"
                     /translation="MVNENVALRAVLFDVDGTLADTERDGHRVAFNAAFEESGLDWNW
                     DVELYGELLEITGGKERIHHYIKNFAPAELERTDLDSWIANLHKIKTRHYVGLLARGG
                     IPLRPGVERLIHELREAKIKIAIATTTTEENVTALLKSTLGEDSLGWFDVIGAGDIVP
                     GKKPEPDIYHWALRELGLSADECIAVEDSENGLRASLAAGLDTVVTVNDYTRLQNFNG
                     ASLVVSDLGEPAQPFAVLQGDAAGRWVDAKLLSALKAGMTV"
     gene            complement(3486..>3703)
                     /locus_tag="VFI43_09650"
     CDS             complement(3486..>3703)
                     /locus_tag="VFI43_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HET7062430.1"
                     /translation="ENIVYIPMARAPEIYARLLYGTLRKLDQESFDRLLVEEAPDEPQ
                     WAAIADRLCRASYINTDKNMETGALHG"
BASE COUNT          878 a         1095 c          904 g          826 t
ORIGIN      
        1 gacgagttat tcactgcctt gtttctccac tgtgctgccc tccaggcatt gctcaatttc
       61 gagcgattgc ttgatttcca acgcgagctg caacgcggcc tcgacatcat cgtcaagcac
      121 cgtgtaatgt cccatcttcc ggccagggcg cgcgctgtgt tttccataca gatgcagctt
      181 gacccccgga tgccgcagga tctgttccca ctctggctcg ccccggctcc acagatcgcc
      241 caaaagattt accatcacag cagcgctatg catggccgtg ctgcctaacg gcattccgca
      301 caagaccctc acctgttgct cgaattgaga ggtgatgcaa gcatcgatcg tatagtggcc
      361 gctattgtgg gggcgcggtg cgatctcgtt cacaagcagt ctaccgtctt caagcacgaa
      421 aaactcggcg cacagcaccc cattataatc aagccgttca gcgattttca tggcagcttc
      481 ccgagcagtc ccgacaattg atgatggaag tcttgcaggc gcaatgctga catcgagaat
      541 acctttgcga tgccgattct cgggaaccgt gaaagcactg gccgcaccat tgaaacctcg
      601 cgctacgatg accgatatct cggttttcaa gggcatcaat ttctccagga cacaaggctc
      661 ccggttcata ttatcgaatg cattcttcag ctcttcgaca tcgttcacga gcgcttggcc
      721 cttgccgtca tagccaaaac ggcttacctt gagtattccg ggaaaaaggt ctccgtacag
      781 gttctcaaat tcacctaatg gcgcttcccg caacccgttg gaagcgccat tagagttcca
      841 ttcgcagtca atcactctga aaggcgcgac ctggaatccg ttgctgctca ggaactgctt
      901 ttcccgaatg cgattctggg caatcgccac gctgtccgca gcggggctga cgttgcagta
      961 cgtggccaaa atcctgagtg cttcggctgg cacgttttca aattcagtgg taacagccgc
     1021 gcaggtagag cccagctctc gcaatgcact tgggtcaagg taatccgcag ccagatggcg
     1081 atcggcaatg ctgcttgtag ggccgttgtc gtcgggatcc aaaacggtga cacggtaacc
     1141 catgctttga gccgccatgg taaacatgcg acccaactgg ccaccgccga gtatcccgag
     1201 cattgccccg gggttgatgc tcatgattcc ggcaacacca ttgcctcaac cgtggcttgc
     1261 tgagcggcgc gaaactcatc aagccggcgc gcaagacccg cgtcgctaat cgctaaaagc
     1321 gctaccgcaa atagcgctgc attggaggca cccgcctcac ctatcgcaaa cgtcgcgaca
     1381 ggaacgcctt ttggcatctg aacgatggaa agaagagaat caagaccttg aagctgcctg
     1441 gaagtcacag gcacgcccag cactggcaga atggttttgg cggcaatcat gccaggaaga
     1501 tgcgcggccc cgcccgcacc ggcaataatg cacttgaggc cgcgggtcgc ggccgatcct
     1561 gcatattcaa acatacggtc aggcgtgcgg tgggcggaaa caaccctggc ttcccaggga
     1621 acaccaaagt ccttcaacac ctcaaccgag tgcttcatga cctcccagtc gctattgctg
     1681 cccatcacca cgccaactac gggctttccc attcaggtcc ttttcttaat cttgcaacaa
     1741 gatagtcttt tagcactgcc agtaaaccag atatcggctt tgttctgcca tgaagataat
     1801 acgtgcgaaa acgctttgat ttccctaccc acgaacctca aaccagtcct cgacgcaccg
     1861 tcatttccac gaaagcggaa atccgtcgct ttgaacctta ccgctacgtg cagatctgga
     1921 cacctgcttt cgcgggtatg acgcgtacaa caaactcaga cagcattccg tgtacccgtc
     1981 tccccaaaag gagagacggc actgatgatg ttccagggtc tcaagccagc gcgtcacgcg
     2041 cccaggtctc ttacgaccag atgcagaggg agtcaacgga ccaacacaac ccttttccgg
     2101 atgcctgaat gcgagagaat aaatatatcc ttaaacgcac ttgagcgagc taaaacgggc
     2161 tatttgcgaa gaggggtatg ttcgtccgtg gactccccaa tatcttccat gtcttcgatg
     2221 tagggatagc gcacgtgtcc atagcgtatg acaagtactg ccaccgcaat caggacgatc
     2281 gaagcggata cgccgagcaa ccggacatta tccatctcct tgatgtcaac gattacgtag
     2341 cgcgccattg ccaccatggc aatgtaaaga gggaatcgta ccggaagctt gcccgactcg
     2401 aaatagagac ccaccatggt caaaatctca aggtagagaa acatcagcag aagatcggcc
     2461 agcgtcactc tggcggtcct gatcatggac agcgtttctt gataacctgc aaatatcgtg
     2521 gcaatggcta taacaataag cccggcgccc tcaaaaatat aaagggtgcg gcgggaaaga
     2581 ttcgcggcgc ggggatggag catcttgttt ttcttgttgg gggaatgatc agtgaagcaa
     2641 gaatgatatt tctatgagat gacaccctga tttcaattca ggttctgtcg taaaaccacg
     2701 cctcccatct gactcagacc gtcatcccgg ctttgagtgc tgaaagcagt tttgcatcca
     2761 cccaccggcc cgcagcatcg ccttgcagca cagcgaatgg ctgagccggt tcgcccagat
     2821 cggatactac cagagaagca ccgttgaaat tctgcagccg ggtatagtca ttgaccgtca
     2881 ccacggtatc gagtcccgcc gcaagcgatg ccctcaggcc attttccgaa tcttccactg
     2941 caatacactc gtcagcggac aagccgagct ccctgagcgc ccaatgataa atatccggct
     3001 caggtttctt gcccggcacg atgtcgcccg ccccgataac gtcgaaccag ccgagagagt
     3061 cttctcccag cgtcgacttc agcagtgcag tcacattttc ttctgtcgtg gttgttgcaa
     3121 tcgcaatctt gatttttgcc tcgcgtaact cgtgaatcag gcgctcgacg cccggacgca
     3181 aaggaatgcc cccgcgcgcc agcaggccca cgtaatgcct ggtttttatc ttgtgcagat
     3241 tggcaatcca gctatccagg tccgtacgct ccaattctgc cggagcaaaa tttttgatgt
     3301 aatgatggat gcgctctttc cctccagtta tttcaagcag ctcaccatac aattcgacat
     3361 cccagttcca gtcaaggccg gattcttcga acgcggcatt gaaagcgacg cgatgcccat
     3421 cccgctcggt atcagccagc gtgccgtcca catcgaaaag cacagctcga agtgctacat
     3481 tttcattaac catgaagtgc tccagtttcc atatttttat cggtattgat gtagctcgca
     3541 cgacagagcc gatccgcaat agccgcccat tgcggctcat caggcgcttc ttccaccagt
     3601 agacgatcga aactctcttg gtcgagcttc cgcagcgtcc cgtagagcag ccgagcgtaa
     3661 atttccggtg cccgcgccat cggaatataa acaatatttt cgt
//
LOCUS       DASSHJ010000249         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_634780, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000249 DASSHJ010000000
VERSION     DASSHJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634780"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFI43_09655"
     CDS             complement(<1..501)
                     /locus_tag="VFI43_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7062431.1"
                     /translation="MIAKFLFAAFLAVSTAVSAQLPDFTELVEKQGAAVVNISTVQGP
                     QGSAGGRNFPGIPNMPNIPEDDPFFDFFRRHMPPPGSPRDFEARSMGSGFIISSDGYI
                     LTNTHLVDSVDEINVKLTDKREFRAKLIGADRKTDIALLKIDAAGLPKVTQGDPNKLK
                     VGEWVVA"
     gene            complement(595..1554)
                     /locus_tag="VFI43_09660"
     CDS             complement(595..1554)
                     /locus_tag="VFI43_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HET7062432.1"
                     /translation="MAAANVPPVFAQNSSNSSPEALNWLEKIASAPRRYNYAGTFVYS
                     AGGDIETSRIIHLVDNEGEHEKSEVLDGTPREVVRNNDELKCYLPESKTVVLEKRWLR
                     KVFPALLPQSVSDLDHNYIIKMGGQERVSDHVCQVIELEPKDDMRYGQKLWVDPDTGL
                     IIKAAVLDKDRVVEQFVFTQLKIGGEIDNGLLKSKYAAKAGQWRTTNLISSAGGTGRL
                     GWQIKDPPPGFKKIVEMKRNLPGKSVPVYHIALSDGLAAVSVFIEPVSKHSSPPGEGL
                     YHGRGAINIYTRTVSDNTVTTVGEVPPATVVKIGNSVINNKAD"
     gene            complement(1584..2132)
                     /locus_tag="VFI43_09665"
     CDS             complement(1584..2132)
                     /locus_tag="VFI43_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HET7062433.1"
                     /translation="MKEKLSALMDGELDSDDTAGILAGLKQTDELREQWAAYHLISDT
                     LSQPEVMRIDISNRVMARLAAEPTALAPRATARHRPVAFAAAASIAALAVAGWMSLHT
                     TPPLALPQANLADNGTPPATSLTALPATPVAGVPYAPVSAPERMNDYLMAHREFSPGA
                     GMHGAPPPYVRTVSEPRQNFAR"
     gene            complement(2135..2740)
                     /gene="rpoE"
                     /locus_tag="VFI43_09670"
     CDS             complement(2135..2740)
                     /gene="rpoE"
                     /locus_tag="VFI43_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HET7062434.1"
                     /translation="MGDREIDQQLVERAQHGDKHAFDLLVVKYQRKLARLLSQFIRDP
                     TEVEDVTQEAFVKAYRALPSFRGDSAFYTWLYRIGINTAKNFLVAQGRRAPTTTTGGL
                     DSEEAEGFEEASNLREMNTPESELTSKQIAQTVNQTLEELPEELRTAITLREIEGLSY
                     EEIAAIMNCPIGTVRSRIFRAREAIAEKLRPLLGTGKDKRW"
BASE COUNT          668 a          870 c          854 g          801 t
ORIGIN      
        1 cgccacaacc cattctccga cctttaactt gttgggatct ccctgtgtca ccttcggcag
       61 acccgcggcg tcgatcttga gcagcgcgat gtctgttttg cggtcggcgc ctatcagttt
      121 cgcgcgaaat tcacgcttgt cggtcagctt gacattgatt tcgtcgacag aatccacgag
      181 atgcgtgttg gttagaatat agccgtcgga actgataatg aatccggagc ccatcgacct
      241 cgcctcgaaa tcccgaggag acccaggggg cggcatatgg cgacgaaaga agtcgaagaa
      301 aggatcatct tcgggaatgt tgggcatatt gggaatgcct ggaaaattcc ttcctcccgc
      361 gctgccttgc ggaccttgca ccgtgctgat gttgaccact gcagcgccct gtttttccac
      421 cagttcggta aaatcgggca attgcgcgga aacggccgtg gacactgcca ggaacgctgc
      481 aaaaagaaat ttagctatca ttctctgcct tcagaaaaat aaatataagt aaatccgctt
      541 tgtaagagca acggcggcca tcgaatagca ggcttcctga acgaacgccg gttgctaatc
      601 cgctttatta ttgataacgg agttcccgat ttttactacc gtggcaggcg gcacctcgcc
      661 taccgtggtc accgtgttgt ctgaaacagt tcgggtatag atgttgattg cgcccctgcc
      721 gtgataaagc ccctcgccag gaggcgaaga atgtttggat accggttcga taaaaaccga
      781 gacagcggcc aagccgtcgg acagcgcgat atggtagacc gggacggact tcccgggaag
      841 attgcgtttc atttcgacga tttttttgaa cccagggggt ggatccttga tttgccagcc
      901 gagcctgcct gtcccacccg cggaagatat cagattggtt gtgcgccact ggcctgcttt
      961 tgccgcatat ttcgatttta gcagcccatt gtcgatctcg ccgccgatct tgagttgagt
     1021 gaagacgaac tgttcgacga cgcgatcctt atccagtact gctgccttaa ttatcaggcc
     1081 ggtatcgggg tccacccaga gcttctgccc gtagcgcata tcgtccttgg gttccagttc
     1141 gatgacctgg cagacgtgat cgctcacccg ctcctgaccg cccatcttga tgatgtagtt
     1201 atggtccaga tcgcttacgg attggggaag aagcgccggg aaaacctttc ttagccagcg
     1261 cttttccagg acgaccgttt tgctctccgg gaggtagcat ttcagctcgt cattgtttcg
     1321 gacaacttct ctcggcgtgc catccagcac ttcacttttt tcgtgctccc cctcgttatc
     1381 cacgagatgg ataatccggg atgtttcgat atcgccgcca gcggaataaa cgaaagtccc
     1441 ggcatagttg taccggcggg gcgcgctcgc tatcttttca agccagttga gcgcttcagg
     1501 agaggaattc gatgagtttt gcgcgaatac gggagggaca tttgctgctg ccaagagcaa
     1561 ccaggcagcc aatgcaatac gcgtcatctg gcgaagtttt gacgcggttc agagaccgtg
     1621 cgtacatagg gagggggagc gccgtgcatt ccagctcccg gtgaaaattc cctgtgcgcc
     1681 atcaggtagt cgttcattcg ttcgggggcg gagaccggag cataggggac gccggcaacc
     1741 ggagtggctg gcagcgcagt caaggaggtc gccggaggcg ttccgttgtc cgccagattt
     1801 gcttgtggca gagcgagcgg gggtgtggtg tgaagactca tccatcctgc cacggccagc
     1861 gctgctatcg atgctgctgc ggcaaatgca accggcctat gccgggccgt tgcacgagga
     1921 gccagtgccg tgggctcggc agcaagccgg gccatgacgc gatttgagat gtcgattcgc
     1981 atcacctccg gctgcgacaa ggtgtcgctg atcagatggt aagcggccca ttgttcacgc
     2041 aactcgtcag tttgcttcaa tcctgccagg atacccgcag tatcgtcgga atccagctca
     2101 ccatccatca atgccgataa tttttccttc atgcttacca cctcttatct ttgccggtcc
     2161 ccagcagggg acgcaatttt tccgctatcg cctcccgggc acgaaatatt cgcgaccgga
     2221 ccgtgccgat ggggcagttc atgattgccg caatttcctc atagctcagc ccctcgattt
     2281 ccctcagtgt aattgccgtt cgaagctctt caggcaattc ttccagcgtt tggttcaccg
     2341 tttgagcgat ctgcttgctc gtcagttcac tctccggcgt attcatttct cttagattgc
     2401 tggcttcctc gaagccttcc gcttcttcgc tgtcgagtcc gcccgtcgtc gtggttggcg
     2461 ctcggcgtcc ctgcgccacc agaaaattct ttgccgtatt tatcccgatc cggtacagcc
     2521 aggtataaaa agcgctatcg ccccggaaag acggcaatgc gcgataagcc tttacaaaag
     2581 cttcctgggt gacgtcttcc acttccgtcg gatcgcggat aaattgcgac aagagccgcg
     2641 cgagcttgcg ctgatacttt acaaccagca ggtcgaacgc atgtttgtcc ccgtgttgtg
     2701 cgcgttccac cagttgctgg tcgatttcac ggtcacccat gccgccagtt cctgaatttt
     2761 tgatttttac ttgtactgat gtgctgaaac atcaggcaag tatacccgtt caggcagcta
     2821 tcggggaatc gtgcacgccc gccgtacgtg actgagttaa cagacaatga acagtataaa
     2881 ttagttcaga tgaaaacgtg gttattgaaa aacatgaaac ggatgggtta ttttgctgct
     2941 catgcctgag agcaggagtc ggacgtactg cgctggagaa gcaaagcgtt ccgccagctt
     3001 tctgctttct ggctatcaag acgaagcggc ctgcttctcc tggcctcccc ttcaaatgga
     3061 atcgaatcgg ggtgccggag aatgaaaaca aggaatggaa cattttttaa agttttttta
     3121 ttgttgttta ttgctgctta ttgttgtttt taccgacccc catcttccat ggcgtcgaat
     3181 caaaggcgcc cca
//
LOCUS       DASSHJ010000250         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1119398,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000250 DASSHJ010000000
VERSION     DASSHJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119398"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /gene="prfB"
                     /locus_tag="VFI43_09675"
     CDS             <1..120
                     /gene="prfB"
                     /locus_tag="VFI43_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964773.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7062435.1"
                     /translation="VLDQSRIKDLRTGIETGNTQAILDGDLDGFIEASLKQAV"
     gene            170..1687
                     /gene="lysS"
                     /locus_tag="VFI43_09680"
     CDS             170..1687
                     /gene="lysS"
                     /locus_tag="VFI43_09680"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7062436.1"
                     /translation="MQDPSSQEAADRKGSDLKLVEERRAKLATLREAGNPFPNDFRRT
                     DLAAELHHSYADYSHERLEAEPRMVSVAGRMMLKRVMGKASFATVQDMSGRIQLYIAR
                     DIAGEEAYSAFKHYDLGDILGAAGTLFRTKTGELSVRVTHLRLLTKTLRPLPEKFHGL
                     TDQEQKYRQRYLDLVTSEDSRKVFITRSRIIQAIRECLAARGYLEVETPMMHPIPGGA
                     AARPFITHHNALDTDLYLRIAPELYLKRLVIGGMERVFEINRSFRNEGISTRHNPEFT
                     MLEFYEAYQDYAYLMNLTEIMLREISEKVLGTAKITYQGREIDLAQPFERLTIAQAIE
                     KFHPEYSEAQLGDHAFLISRLKALGVPFKPEDGVGGLQLTLFDETTEHLLFEPTFIID
                     YPAEVSPLARRNDSNPAVTDRFELYIAGREIANGFSELNDPDDQKARFQEQARAKEAG
                     DVEAMYYDEDYIRALEYGLPPTAGEGIGIDRLVMLFTDSPSIRDVILFPQLRRED"
     gene            2243..2797
                     /locus_tag="VFI43_09685"
     CDS             2243..2797
                     /locus_tag="VFI43_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062437.1"
                     /translation="MGKLSNTPEGYVVFIILSALLLPAKLANALSLSEYHKEAVSTSC
                     PVATWNIIYKTCRAPACGTEQYGQGIRNRLCSTSECGEESKTPGTYTSCRNLDNDIDD
                     SRIIAAVNSGKGVDRAAALLAKAIKETENLEIAVRDEITILLKDVTMDSTTAEEKIDS
                     LRTLLLKNGSATTVDEVRETWVKA"
     gene            complement(2833..3075)
                     /locus_tag="VFI43_09690"
     CDS             complement(2833..3075)
                     /locus_tag="VFI43_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062438.1"
                     /translation="MAYRGHGGSQILAQRDSLALWRLTPDLKRYLKLTNVSPDISRQY
                     GKVNFEQIFLLALSEDHNLQERIWNNRPLKSGNRYR"
BASE COUNT          812 a          875 c          861 g          702 t
ORIGIN      
        1 gtgctggacc agtcgcgtat caaggatttg cgaacgggca ttgaaacagg aaatactcaa
       61 gctatcctcg acggcgatct ggatggattc atcgaggcca gcctgaaaca ggccgtatag
      121 gagcgtggtg gatcataata attcagaaaa aagtccgcag gacaaaaatt tgcaggaccc
      181 gagctcacag gaggctgctg accgtaaagg ctccgacctg aaactggtcg aagagcgccg
      241 ggccaagctg gcaacactcc gtgaggccgg caatcccttc ccgaacgatt ttcgccgaac
      301 cgacctggcg gcggaactgc atcatagcta tgcagattac tcccacgaga ggctggaagc
      361 cgagccccgg atggtcagcg ttgcagggcg catgatgctc aaacgcgtga tgggcaaggc
      421 gagctttgcc actgtccagg atatgagcgg gcgtatccag ctatacatcg ctcgcgacat
      481 cgccggcgaa gaggcttatt ccgcattcaa gcattacgat ctgggtgaca ttctcggggc
      541 ggcgggaacg ctgttcagga ccaaaactgg cgaactatcg gtgcgcgtca cacatttacg
      601 cctgctcacc aaaaccctgc ggcctcttcc tgaaaaattc catggtctaa ccgaccagga
      661 acagaaatac cgccagcgct atctcgatct cgtcacaagt gaggatagtc gaaaagtgtt
      721 cattacgcgt tcacggatca tccaggccat ccgcgaatgc ctggccgcgc gtggctacct
      781 agaagtcgaa accccgatga tgcatcccat ccccggggga gcggcggcga gacctttcat
      841 cacgcaccat aatgccctcg atacagattt gtatcttcgc atcgcaccgg agctttacct
      901 caaacgcttg gtaatcggtg gtatggagcg ggtgttcgaa atcaaccgga gcttccggaa
      961 cgagggaatt tccactcggc ataatccgga attcaccatg ctggagttct atgaggccta
     1021 ccaggattac gcatatttga tgaacctgac cgagatcatg ctgcgcgaaa tctcggaaaa
     1081 ggttttgggt accgcaaaaa taacctacca ggggcgggaa attgatctgg cccagccgtt
     1141 cgaacggctt accatcgccc aggctattga gaaattccac ccggaatatt cggaagcaca
     1201 gttaggcgac catgcttttc ttatctctcg actgaaggcg cttggcgtgc cgttcaaacc
     1261 tgaagatgga gtcggcggac tgcagctcac gttgtttgat gaaaccaccg agcatctgtt
     1321 gttcgagccg accttcatca tcgattatcc cgctgaagtc tccccccttg cgcgacgcaa
     1381 cgactccaac ccggccgtca ccgaccgctt cgagctttac attgcagggc gcgaaatcgc
     1441 caatggcttt tccgaactga atgatcctga tgatcagaag gcgcgcttcc aggaacaggc
     1501 gcgcgcgaag gaggcaggag atgtcgaagc catgtactac gacgaagatt atatccgcgc
     1561 cctcgagtac ggcctgccac ccaccgcagg ggagggaatt ggcatcgatc ggctcgtcat
     1621 gctcttcacc gacagtccga gcatccggga tgtcattctg tttccacagt tgcggcgtga
     1681 ggactgaacg agtcgtgaaa gtttcagctc cctctggctg tacgcccctg caaaccccca
     1741 gcaattcata agcgcaccgc actcaaaggc agaacgaatg cgccgcaaat cggcggcgcc
     1801 aggccgattg ctgagagccg aagcggctga acttgcgtta cttcatggga acaaggttag
     1861 tgctgcagcg cagtcaatgc ctgttcctgg ccaagttctt cgctcaattc ccgatacttc
     1921 agatcgtctc gccaggcaag tgggtcacgc tggatgcgga gtctggggcg ttgtatgcat
     1981 gatctgccca cgggaattgt tcggaaccgg aaacatatct ccactatcat taagtatacg
     2041 cggagcataa aaaacactgg tctcgtataa gcgtgtcggc tgagagggat taggatcgat
     2101 caaaaccatg agaatgttca attatgcgta acactttctg gttttgcgtc ctcggatact
     2161 catgatcgat tacggataac attccgaata ttgctaacaa aacaacccgc tggttgcgca
     2221 catcgagcga aaggaggctg aattgggcaa gctatccaac accccagagg ggtatgtagt
     2281 ttttattatc ctttcagcct tactgctgcc agcgaaactg gcaaatgccc tctcactttc
     2341 cgagtatcac aaagaggcgg tatccacgag ttgcccggtg gctacatgga acattattta
     2401 taaaacatgc agagcaccgg cttgtggcac cgagcagtat ggccagggca ttcgaaatag
     2461 gctttgctcc acctcagaat gcggcgaaga aagcaaaaca cccggcactt acacgagttg
     2521 tagaaatctg gataacgaca tcgatgattc gagaatcatc gctgcggtga actcaggaaa
     2581 gggtgtcgat agagcggcgg cgctactggc gaaagctatt aaggaaacag aaaatctgga
     2641 aattgccgta cgtgatgaaa tcacaatcct cctcaaagat gtcacgatgg attcgacgac
     2701 tgctgaagag aaaattgatt ccctgagaac actcttgtta aaaaacggat cagcgacgac
     2761 ggttgatgaa gtacgcgaaa cttgggtgaa ggcataaata gcagcgcatc ccacccaaga
     2821 gcctgctccg tctcatctat agcgatttcc agatttaagg gggcgattgt tccagatccg
     2881 ctcctgcaag ttgtgatcct cgctgagtgc caacaggaat atctgctcga aattcacctt
     2941 cccatactgc ctggaaatat cgggcgagac attcgttagt ttcaggtatc gcttgaggtc
     3001 aggggtcagg cgccaaagcg cgaggctatc tcgttgggcg agaatctggc ttccgccatg
     3061 tccgcgatag gccatgacca gatcggcggg atgtacgccc tgggccgccg ttctccgaaa
     3121 ggctcagttg cagtgcttct tcctcactta ctacgcccgc aatcgacttc atggtctttc
     3181 gacaagcgtc cctccgctat cagtgattgc agtgcaacaa ggcgccggcc tcctgtggca
     3241 acttagttct
//
LOCUS       DASSHJ010000251         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1030183,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000251 DASSHJ010000000
VERSION     DASSHJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030183"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFI43_09695"
     CDS             complement(<1..798)
                     /locus_tag="VFI43_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7062439.1"
                     /translation="MKFITSRHNAFFKQLMEVGESAQERKAAGLTLLDGIHLIAAYRQ
                     AVGQPKRLIISETGWETSEIKCLLAEESAAGEEVVVLTDNLFQIASPVKTPTGIMALI
                     PIPEPEEIPLRASQRNEPFSVLLEAMQNPGNLGSILRSAAAAGAGQVYLSVGCADVWS
                     PGALRAAMGAHFLLRIYEQSDLANIAKTFNGKVVATSLKAKTSLYQTALTGPIAFVFG
                     NEGAGLSDEVLAATNEQIIIPMPGGTESLNAASAAAVCFFERVRQMKA"
     gene            complement(883..1476)
                     /gene="greB"
                     /locus_tag="VFI43_09700"
     CDS             complement(883..1476)
                     /gene="greB"
                     /locus_tag="VFI43_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET7062440.1"
                     /translation="MSKAFTREEYQAPDEQEANDPALPALPAGSRNYITPSGYARLMD
                     EFFWLMNKERPRVTATVSWAASNGDRSENADYIYGKKRLREIDRRIRFLTKRLDIAEV
                     IDPAAVREDTERIFFGATVTFVNQKGEKKTVSIVGVDEIDTARGYISWISPMARALIK
                     AREGDVVILNAPGGLQELEILEVKYQTIEIEPFIGEE"
     gene            1632..2897
                     /locus_tag="VFI43_09705"
     CDS             1632..2897
                     /locus_tag="VFI43_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET7062441.1"
                     /translation="MESGESKNSQPFWHYLFAAPHRPMFLAGTLQGVLTLAWWVFNLV
                     ARQQGTAAPGWSVAPSSAHAFLMIYGFFSFFIFGFLFTTFPSWMNGEKIRPRYFISSC
                     LSMVIGSSLFYLGLVTGKAIMAAGVFLLLAGFTGGAFALLQVLFRGGKQDKRHSIAAS
                     GGLVGGWIGIFSYLLWLLTDNRALGEFSRTAGIWFFILPIIMTVSHRMIPFFSSRVLE
                     NYVVVRPYWILWIMIACSVGHGLLQLQEAGPRYAWLLDFPLAVCAAYLSFTWGLLRSF
                     RVRLLSVLHASFAWLVVAMLLYAVQGLVLFTSGGTSWIWGLAPLHALGIGYFAGMVLA
                     MASRVTLGHSGRPLVMDEFTWRVFLGFQFAALFRVLPDTLTTALPAATQMAPGLYLLA
                     GLAWLICFTLWAIRFAPIYWRPRVDGKSG"
     gene            complement(2932..3063)
                     /locus_tag="VFI43_09710"
     CDS             complement(2932..3063)
                     /locus_tag="VFI43_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7062442.1"
                     /translation="MKAFGSKGRFQAVMEKIPVHVIVNSKAGLLGAAQEARRLIAER"
BASE COUNT          607 a          889 c          785 g          787 t
ORIGIN      
        1 cgctttcatc tgccgtactc tctcaaaaaa acataccgct gcggctgatg ctgcattcag
       61 ggattccgta ccacctggca tagggataat aatttgttcg ttcgtggccg ccaatacctc
      121 atccgatagg cccgcgcctt cgttgccgaa aacgaatgcg ataggtccgg tcaaagccgt
      181 ctggtagaga ctcgttttcg ccttcaacga agtcgcgacc accttgccat tgaaggtctt
      241 cgcaatattt gcaagatcgg actgctcgta tatgcgcagc aaaaaatgag cgcccatggc
      301 ggcgcgcagc gccccagggg accacacatc ggcacatcct acagaaagat agacctgacc
      361 tgccccggcg gcagcagcgg agcgaaggat tgaaccgagg ttgccaggat tctgcatcgc
      421 ttccagcaat accgaaaaag gctcattcct ttgggaggca cgcaggggaa tctcttctgg
      481 ttcgggaatg ggtatcaggg ccatgatgcc ggtgggtgtt ttaaccggag aagcgatctg
      541 aaacaggttg tccgtcagca caacgacttc ctcacccgcc gcactctcct ccgccagcaa
      601 gcactttatc tcgctggttt cccaaccggt ttcgctgata atcaggcgtt tcggctgccc
      661 caccgcctgc ctataggctg cgatcagatg gatgccgtca agaagcgtca atcctgcggc
      721 cttgcgttct tgcgcagact ctcccacctc catcaattgc ttgaaaaatg cgttatggcg
      781 agaagtgatg aatttcatcg gaaaacactt cctctccctg gagtttcaat tctctggcac
      841 atcgagtttc gtgcccggca gacctgcaat aaccccatgc gctcactctt ccccgataaa
      901 tggctctatc tcaatcgtct ggtatttaac ctccagtatc tccagttcct gaagtcctcc
      961 tggcgcattc aatataacca catcgccttc ccgcgccttg atcagtgcgc gcgccatcgg
     1021 cgaaatccag ctgatgtaac ccctggccgt atcgatttca tcgacaccca cgatggaaac
     1081 cgtctttttt tcccctttct gattcacgaa ggtaaccgtg gcgccgaaaa aaatacgctc
     1141 cgtatcctct cggacggcag cagggtcaat tacttcggca atatccagtc tcttggtgag
     1201 aaagcggatg cgcctgtcga tttcacgcag gcgcttcttg ccatagatgt aatcggcatt
     1261 ttcagaacga tcaccattgc ttgccgccca cgagaccgtt gcggttacgc gcggacgttc
     1321 cttgttcatg agccagaaaa actcgtccat caatcgggca tatccgctag gggtgatgta
     1381 gtttctgctt cccgcgggca gagcgggcaa tgcgggatcg ttcgcttcct gttcatccgg
     1441 agcctggtac tcttctctgg taaatgcttt gctcatatcc tgtatagttt accgaaaatg
     1501 tttccacgtt taggatcgtt ggcgcacaca tcgtcctgca tcagcgagac ctagtggagc
     1561 aagaagatca ttcgaaataa agaattccgc tcaatcggcc gggcaaaccg gacctgatcc
     1621 aagccttata tatggaaagc ggcgagtcga aaaatagcca gcctttttgg cattatcttt
     1681 ttgcagcgcc ccaccgcccg atgtttctgg ccggaacgct gcaaggcgtt ttgacgctcg
     1741 cctggtgggt attcaatctc gttgcccggc aacagggaac ggctgcgccg ggatggagcg
     1801 tcgccccgtc atccgcccat gcattcctga tgatctatgg ctttttttcg ttttttattt
     1861 tcgggtttct gttcaccacc ttccccagct ggatgaacgg tgaaaagatc aggcctcgct
     1921 acttcatcag ttcctgccta tcgatggtga taggaagctc gcttttctac ttgggactcg
     1981 taaccggaaa ggccatcatg gcagcaggag tcttcctctt gctggctggc ttcaccggag
     2041 gggctttcgc gcttcttcag gtgttgtttc gcgggggcaa acaggacaag cggcattcta
     2101 tcgcggcaag cggcggactc gttggcgggt ggatcggaat attctcctac ttgctatggc
     2161 ttttgaccga taatcgcgcc cttggcgaat tttcccgcac ggccggaatc tggtttttca
     2221 ttttgcctat tataatgacc gtgagccacc gcatgattcc atttttttcc agccgcgtac
     2281 tggagaatta cgtcgttgtg cgaccttact ggatactctg gataatgatc gcctgctcgg
     2341 tcgggcacgg ccttctgcaa ttgcaggaag ccggaccgcg atatgcctgg ctgctggatt
     2401 ttccgctggc ggtgtgcgct gcgtacctat cgtttacctg gggactatta cgcagctttc
     2461 gtgtccgcct cctctccgtg ctgcatgcct cctttgcctg gctggtagtt gccatgctgc
     2521 tgtatgcagt acaaggtctt gtactgttta cgagcggggg aacgtcctgg atatgggggc
     2581 ttgcaccgtt acacgccctc ggcatcggct atttcgccgg gatggtgctc gccatggctt
     2641 cccgggtaac cctcggccac tcagggcgac cgctggtgat ggatgaattc acgtggcgag
     2701 tctttctcgg ctttcagttt gccgcgcttt tccgcgtact gcctgacacc cttaccactg
     2761 ccctccctgc agccactcag atggctccag gcttgtacct cctcgccgga ttagcctggc
     2821 tgatttgctt caccctttgg gcaatccgtt ttgccccaat ttactggcgc cctcgtgtcg
     2881 atggcaaatc ggggtgacga gccaggattg gaaatgtcgc acaagttatc ctcaccgctc
     2941 cgcgatcagg cggcgcgctt cctgggctgc gccaagcagg ccggctttag aattaacgat
     3001 tacatgaacc ggaatctttt ccattacggc ctggaagcgc cccttgctgc caaacgcttt
     3061 catgaatc
//
LOCUS       DASSHJ010000252        15907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_40165, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000252 DASSHJ010000000
VERSION     DASSHJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 15907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15907
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40165"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFI43_09715"
     CDS             complement(<1..384)
                     /locus_tag="VFI43_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7062443.1"
                     /translation="MARVLIIDDDGSFRESLAETVSSLGHELFLAADAGRGLSFLKRE
                     EVDLVFLDFRLPDMTGIEVLRAIKRLRRDIPPVIMLTAYTSVDNTIEAMKLGAFEHLA
                     KPVGRQAVENMLNAALASSSRAIRQN"
     gene            complement(369..1823)
                     /locus_tag="VFI43_09720"
     CDS             complement(369..1823)
                     /locus_tag="VFI43_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7062444.1"
                     /translation="MRVTLALRWRLLLLWLFMLVVSGALAYIIRDVYQLGSEAQAEKS
                     VEQTIHACVALQTEYSRSVRPGEDIIDRDLMSALLNVILDDLPGVEGGFWHDSSRFVA
                     YAFPSHVGSEEKKDVPPTERNRVEALARKSFVEGKSMADVLAGSHEIVTLAACPVNPE
                     GHLAAWAMMRSPVAAGEAYDEVNRGLGLLLAFVIISGLWLAFSLYGWSKRFTRMERDL
                     AESSRDVPWEMARTGDAELDRIVTAFNQFRTRLDAERAHIAELGTLIERSERFTALGR
                     MAAAVAHEVRNPLAAMQLKAENALVRPNSERMALEFILREIKRLDETVKELLKKAEPI
                     STQFRLVHITDWLMERVGAFEEQGVAASIGLRTEAGVESWTFDPHSLGRALDNLIANA
                     LQHTPSGGIVTVTANKNALGTTMILQVCDTGPGVSANVEPTLFEPFTSGRPAGVGLGL
                     ALAREIAVAHGGEARYLRKASGACFELEIPWHAS"
     gene            complement(1953..2338)
                     /gene="crcB"
                     /locus_tag="VFI43_09725"
                     /pseudo
     CDS             complement(1953..2338)
                     /gene="crcB"
                     /locus_tag="VFI43_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380894.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
     gene            3048..4826
                     /locus_tag="VFI43_09730"
     CDS             3048..4826
                     /locus_tag="VFI43_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET7062445.1"
                     /translation="MKIDKRYRVSRLPEMAMLCISLLVVCAIALISYRNIQFMEAASE
                     QRSVSRDMRYESMRLLSMLVDAETGQRGFLLTGKEQYLEPYHHAVLEIPRSLELLKNL
                     NTSSLDHSMDLGRLERLTSAKLSELNESIELYRSGNATQVHAMLDSGQGKVLMDEIRM
                     TCHGMEEAAQNNLVSFINAQDESTAGLRVVSMVGSGVLFVFLIISAIVISRGMVRREW
                     LYKESEIAKKLLKTTLAGIADGVIATDAHGAITFINPMAQKLTGWNKAEALGRHIREI
                     FSIVTEANEIELDNPLEKALAEGIAVEPSNHANLVSRDGSRLPIDDSAAPLKDENGKL
                     IGAVLVFRDISMRRHSEQQLEEAAAALRRSNEELQQFVNSAAHDLRSPLSTIRGMTEL
                     LSSKFSRQLGEKGEELMNYITRGVGRMTQLLEDLLSFARASHFDAASAKPLPLNDVLT
                     VSLSNLSGEIAESAAAVTADPLPIVAAHETHMVQLLQNLIGNALKYRSQHPRIHISVD
                     QGDGEWIIGVTDNGIGIDPIHAQQIFQPFKRLHGEEYSGSGIGLASCQKIVEGYGGRI
                     WVEAELGRGSKFFFTLPVTGAKALPQ"
     gene            complement(5262..6167)
                     /locus_tag="VFI43_09735"
     CDS             complement(5262..6167)
                     /locus_tag="VFI43_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7062446.1"
                     /translation="MNQQTAHTAGHAPGHGTDHGGGHGHYYVPPPSTYPITGSIALLF
                     MGFGAAFTVNKIPVGYWLLAIGFAILFYMIFVWFRTVARESESGKYNEQVDKSFRWGM
                     SWFIFTEVMFFAAFFGALFYMRVLSVPDLADLQSKLLWPDFTGGWPVSGPGIEEKFTP
                     MGAWGLPALNTLILLTSGVTVTWAHWALKLNKRGQLKLGLFLTFALGFLFVGLQAYEY
                     GHAYSELNLKLTSGAYGASFFMLTGFHGFHVTIGSIMLLVIFCRVLAGHFKPENHFGF
                     EGVAWYWHFVDVVWLGLFIFVYWLI"
     gene            complement(7035..7658)
                     /locus_tag="VFI43_09740"
     CDS             complement(7035..7658)
                     /locus_tag="VFI43_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7062447.1"
                     /translation="MATIDQIIDTAVELGERTSWEAVRLHDVATGLGITLDDLRAHFQ
                     KKDNIVDAWFERADRIMLKAAQACDFPYLAPRERLHRLIMTWLGAFYPYQKTTRQMVY
                     GRLEPGHLQAQAAGLMRVGRTVEWMREAAMHKMPHIPRLLEDSGLAAIYLAAFFRWMT
                     DNSPGSVNTSRFLEGCLLVADKFGRMIQLTSAGSTGPVDRDKAPQSV"
     gene            7906..9000
                     /locus_tag="VFI43_09745"
     CDS             7906..9000
                     /locus_tag="VFI43_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HET7062448.1"
                     /translation="MERLYLSFVSPSVRDFHPSYFAMVMATGIVSIAFDALAFPGIAK
                     TLFILNLVLYLMFCLMLGARTVFFPRNLVAELRTLPRSWLFLAFVIGTNTVGMQFTTF
                     LQAGGLASALWFVALLAWLIFLTVIALNAAALPRESVMQSVNGATLLVIVSTVSLALL
                     GARLLDPERGHAPLFLGLWALWTLGFILYLFVVYWIIRRLFFDCFRMEDWQPPYWICM
                     GAPAIITLAGSEFVIRLPRSQWNGLDQLTLGMSFFAWILGTLWIPYLLVMDVRKLTRI
                     PAPVPLWIKIFPWARYAFGTTHHAYEPPAWSRVFPMGMYTACTFFLAKATGYRLLESV
                     SSCWGWFTLLIWALTLVGAFRSFGKFMANR"
     gene            9205..11058
                     /gene="ftsH"
                     /locus_tag="VFI43_09750"
     CDS             9205..11058
                     /gene="ftsH"
                     /locus_tag="VFI43_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7062449.1"
                     /translation="MDKKFQINFWYIIIAILGILVVQNTYKEYTKVEPIPYSKFHSLL
                     EQDKIAEIAITQNQIYGTLRDKGPDGLKDFVTTRVEPELADKLDKHNVTYTGVVQSTW
                     LRDILSWLLPTALFFGIWIFLIRRMNPGGMTGGLMSIGKSRAKVFVEKETKVTFADVA
                     GVDEAKEELVEVVNFLKDPQGYSRLGGRAPKGVLLVGPPGTGKTLLARAVAGEASVPF
                     LSISGSEFVEMFVGVGAARVRDLFDQARQMAPAIIFIDELDSLGRARGAYAMGGHDEK
                     EQTLNQLLAELDGFDPTTGVVLLGATNRPEILDPALLRAGRFDRQVLVDRPDKLGREQ
                     ILEVHLKKITLSPDVKPEQIAALTPGFTGADLANLVNEAALLATRRRGDAVTMDDFNN
                     AVERIIAGLEKRNRLLNPSERRVVAFHELGHAMVALALPGTDEVHKVSIIPRGVGALG
                     YTIQRPTADRFLMKREELENRMAVMMGGRAAEQVVFNEVSTGATDDLARATDLARSMV
                     LRYGMSDALGNVAYDRERSAFLQPGIPVVQNRDYSEKTANAVDSEVRTLVDHALERAI
                     GILKINRALLDRTAEELLKTETLNTPQIEVLKRQIISGPALPAETATAAVA"
     gene            11574..12962
                     /locus_tag="VFI43_09755"
     CDS             11574..12962
                     /locus_tag="VFI43_09755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7062450.1"
                     /translation="MSFTDLNLHPLILKAIADSGYTSPTPIQEKAIPELLAGHDVLAS
                     AQTGTGKTAAFMLPALHRLAAPSTAPGRGPRVLVLTPTRELALQVSEAAAKYGKYLSR
                     VRVVSILGGMPYPVQNRLLSQPVEILVATPGRLIDHIQRGRIDFSRLEMLVLDEADRM
                     LDMGFIDDVEKIASATPASRQTLLFSATLDTAIADVAARLLKLPKRIQVATQQAKLDN
                     IEQRLHYVDDISHKNRLLDHLLRDAELRHAIVFTATKRDADVLADNLAAQGHEAAALH
                     GDMSQRDRTRTLTKLRRGGLRVLVATDVAARGIDVAGITHVINFDLPKFAEDYVHRIG
                     RTGRAGASGIAVSFASGKDGISLKKIERFTGQRIMSHVIPGLEPRAKPRSSTVNTTRS
                     APAIALKRSRSWTNDNVSRSGGAASPREDARPRSSRDSQTTFGNTQRSFRNGGDFDAF
                     SYPHSRTKRTPS"
     gene            complement(13150..13539)
                     /locus_tag="VFI43_09760"
     CDS             complement(13150..13539)
                     /locus_tag="VFI43_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET7062451.1"
                     /translation="MHLIEAYELMGGEDAIRRLVDRFYDLMDEDPDYYGIRKLHPQDL
                     ASSRQKLFMFLSGWTGGPSLYTAKFGDPRMRTRHMPFSIGIPERDQWLSCMSRAMGDA
                     GLDAQLTKTLSMAFYQTADFIRNQPEE"
     gene            complement(13517..14329)
                     /locus_tag="VFI43_09765"
     CDS             complement(13517..14329)
                     /locus_tag="VFI43_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HET7062452.1"
                     /translation="MSNPQRLRILAIVSLVVLLAAAIAPPIPQPEKYHQFADTRSFFG
                     IPNFWNVISNLGFLLVGIDGLVFLASVSKEKGGHLFIEYRERWPYLILFLSITLTGMG
                     SAYYHLAPDTARLTWDRLPLAMTMTALLSAQLTERVSPRIGLRVLPILVGIGMVSVLY
                     WHWSEQVGAGNLNFYIVVQFYSLLVIVLLAIFFPSRYSRASGIYVALAWYAVAKLAEV
                     GDREIYDLGHLISGHTAKHVLAAIAVYSILQMLKTRKPITLSGELHASYRGL"
     gene            complement(14358..15725)
                     /gene="radA"
                     /locus_tag="VFI43_09770"
     CDS             complement(14358..15725)
                     /gene="radA"
                     /locus_tag="VFI43_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7062453.1"
                     /translation="MVKAKSTYSCSGCGGQTLKWQGQCPHCQAWNTLVEVVAEKAASR
                     FIPISESGRVQNLNEVEAGEELRASTGIAEFDRVLGGGLVHGAVILLGGDPGIGKSTL
                     LLQALCHMSLGTNNTPARDVLYVSGEESPRQVALRAKRMGVDAKAVHLLAEIRLESIQ
                     AEMLERKPDIAVIDSIQTIYSEALQSAPGSVAQVRECAAQLTRLAKSSGTCIILVGHV
                     TKEGSLAGPRVLEHMVDTVLYFEGDTHSSFRLVRAFKNRFGAVNELGVFAMTEKGLRE
                     VANPSALFLSHHGGQVPGSCVMVTQEGTRPLLVEIQALVDEAHAPNPRRLSVGLEQNR
                     LAMLLAVLHRHAGVACFDQDVFINAVGGVKITEPGADLAVLLAIVSSLRNKPLPEKTV
                     VFGEVGLAGEVRPVQRGQERLKEAAKLGFVQAIVPKANKPKQAIPGIEIVGVERVDEA
                     VGKTR"
     gene            complement(15718..>15907)
                     /locus_tag="VFI43_09775"
     CDS             complement(15718..>15907)
                     /locus_tag="VFI43_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7062454.1"
                     /translation="WLPVLRTLGTRPWFGGRMMVSRVNNMFDEKGELVDEQVRVQLQR
                     FLSGFADFVNATPVGQDG"
BASE COUNT         3746 a         4451 c         4217 g         3493 t
ORIGIN      
        1 attttgtcgt attgccctgc tactggaagc caaagctgcg ttcagcatat tttccaccgc
       61 ctggcgcccg accggcttgg caagatgctc aaaggcgccg agcttcattg cctctatcgt
      121 attatcgaca ctggtgtagg cggtcagcat gataaccgga gggatgtcgc gtcgcaggcg
      181 cttgatagcg cgcagcacct cgatgcccgt catatccggc aaacgaaagt cgagaaaaac
      241 cagatcgacc tcctcacgct tcaggaatga aaggccacgc cccgcgtcag cagccaaaaa
      301 tagttcgtgg cccaagctcg acacggtttc cgcgagactt tcgcggaaac tgccatcgtc
      361 gtcaataatc aggacgcgtg ccacggaatc tccagttcga agcaggcacc cgaagcttta
      421 cgcaggtagc gtgcctcgcc tccatgcgca actgcaattt ctcgcgcaag cgccaaccca
      481 agcccgactc cagccggacg ccctgatgtg aaaggctcga acaatgtggg ctctacgttg
      541 gcggaaacac caggtccggt gtcacaaacc tgcaagatca ttgtggtacc gagagcattt
      601 ttgttcgcag tcacggtgac aatgccgccc gatggcgtat gctggagcgc attggcaatc
      661 aaattatcga gcgcgcgacc aagcgaatgc ggatcgaaag tccatgactc gacaccggct
      721 tctgtccgca agccaatgct ggcagccacg ccttgttcct caaaagcacc gactctttcc
      781 atcagccagt ctgtaatatg gacgagccgg aattgggtgc tgatgggttc ggcctttttc
      841 agcaactctt tcacggtttc atccagacgc ttgatctcgc gaaggatgaa ttccagggcc
      901 attcgctcgc tattcgggcg cacgagcgca ttctccgcct taagttgcat tgcggccagc
      961 gggttacgaa cctcgtgagc tactgctgca gccatccgcc caagcgctgt gaagcgttct
     1021 gaacgttcga tcagcgtgcc tagttccgcg atgtgcgcgc gctccgcatc aaggcgagtc
     1081 cgaaactggt taaatgcggt gacaatgcgg tcaagctctg catcacccgt ccgcgccatt
     1141 tcccacggca catccctgga gctttccgcc aaatcccgtt ccattctggt aaagcgcttg
     1201 ctccagccat agagactgaa ggccagccag agacccgaga ttattacgaa ggcaagcaac
     1261 aatccgaggc cgcggtttac ttcgtcatac gcctcgcctg cggcaactgg cgaacgcatc
     1321 atcgcccagg cagcgagatg tccttccgga ttcacagggc aggcagccag ggttactatt
     1381 tcgtggcttc cagcaagcac atcggccatg gacttgccct caacaaagct cttgcgcgca
     1441 agcgcctcga cccggtttcg ctcggtaggc ggaacgtctt ttttttcctc gctacccaca
     1501 tggctgggaa aggcataagc gacaaatcta ctcgaatcat gccaaaaacc accctcgacg
     1561 ccaggcagat cgtccagaat aacattcaga agggcgctca tcagatctct gtcgataata
     1621 tcctcccccg gcctgaccga tctcgaatat tcggtctgga gggcaacgca ggcatgaata
     1681 gtctgttcca cggatttctc ggcttgtgcc tcgctgccga gctgatagac atcgcggatg
     1741 atataggcaa gcgcgccgct tacaactagc atgaacagcc agagcaataa taagcgccat
     1801 cgaagagcca gagtgactcg caagcctatt ccttgcaaaa atgttgcgtt atttgaacaa
     1861 gacgatgctc gctatgctcg ctcacataaa acttcggaaa tattcgctgc attactagtc
     1921 tcccatttca agttcgctaa cgagtatgcg agctaatgct gtgccaacga tttaatggtc
     1981 actatcccga gtactgtcat taccaaagac cggccacatg cgaaatgatg atggtgaaag
     2041 cccagccata ttgctcacgc agtaaaaggg caacagtttc ggctgagaag gtcgaaaacg
     2101 tggtcagtcc gccgagaaag ccagtgataa tgaacagcct ggactctggc ggcagagcag
     2161 tattgtgaga aaaaaactca acagcaacgc cgacaagata gccgccaata atattggcag
     2221 ataaggtacc aaaaggaaga gtaggaaaca ccggattcaa catgaacccc agtccccaac
     2281 gcaaccatgc ccctagcgcg gcgcccattc ctactgcgat gacagcgtaa atgctcattc
     2341 tccatcctca gcgcgtgacc aatcgcaagg ataccgcaac tacggatctc ggcattacga
     2401 taagtcctgc aggaggacga tgccgcttgt tgtgctttaa aaaacctgaa agtgatgcac
     2461 tggggaaaac cggtttgctt actgtgctcg ttgcgagcag agggtcttct cgaactgtaa
     2521 tcgcctagcc tatggataac aaatgtcgct ttcgattgat gacggccgcg taaaacggac
     2581 ctatcattag caaaatacaa tcagttgctg aatttcgggc aggacatttt tctgctttac
     2641 catcctcggc gcgataaagg cgcactaatg atgcctggca atgacggaag catcgcctga
     2701 agccccaaaa aaaccgccaa accgagtata tgaagacaat cccggactca gattgagatc
     2761 tccgtcttgt accccttgcc cctagctcgg atggtgacgg tgcgaataat cccagcccga
     2821 gaaggtattg gacttacagc ggtttatccc atgccccatc gctgttatta tgtcagagac
     2881 atgaaagata gaaattgcgc attcgccgat ttcccaatca acccgctagc aaaaattcgg
     2941 gcatgatgtg atcctgaaat cgtcgaggct atccagaagt gttgctggaa cagccgttga
     3001 actattaatt ggttcaacaa ggaggtaagg agaaagcctc cccaaatatg aaaatcgaca
     3061 agcgataccg ggtttcccgc cttcccgaaa tggcaatgct ctgcatttcg ctgcttgtag
     3121 tctgcgcaat tgccctgatc tcctatcgaa atattcagtt catggaggcc gcaagcgagc
     3181 agcgtagtgt cagccgggac atgaggtacg agagcatgag actgctgtcg atgctcgtag
     3241 atgctgaaac gggccaaagg ggatttcttc tcaccgggaa agagcaatac cttgaacctt
     3301 atcaccatgc agtcctggaa attcctcgat cgcttgagtt gctcaaaaac ttgaatactt
     3361 cgagtcttga tcacagcatg gatttaggta gacttgagcg gctgacctcg gccaagctta
     3421 gtgaactgaa cgaaagcatt gaactgtacc gctcaggcaa tgcgactcag gtccacgcga
     3481 tgctggattc gggccaggga aaggtgctca tggatgagat caggatgact tgccacggga
     3541 tggaagaagc tgcccaaaat aatcttgtct catttattaa tgcccaggat gaaagcacgg
     3601 cggggttgag agtcgtaagc atggtaggca gcggcgttct attcgtgttc ctcattatct
     3661 cggccatcgt catatcgagg ggcatggttc gccgggaatg gttatacaaa gaatccgaaa
     3721 tagccaaaaa gctgcttaag acgaccttgg ccggcattgc cgatggcgta attgcaacgg
     3781 acgcccatgg tgcgattact tttatcaatc ccatggctca gaagctgacc ggatggaata
     3841 aggcggaagc cttgggacgg catatccggg agatattctc tattgttacc gaagccaacg
     3901 aaatcgagct cgataatccg ctcgaaaaag cgctggcgga aggtatcgcg gtagaacctt
     3961 ccaatcacgc taatctggtc tcccgggatg ggagtcgcct tcccattgat gacagcgcgg
     4021 cgcccctcaa ggatgagaat ggcaagctga taggcgctgt ccttgtgttc cgcgatatct
     4081 ccatgagacg gcattcggaa cagcaattgg aggaagcagc tgcggcattg cgcagatcga
     4141 acgaagaact gcagcagttt gtaaattccg ctgcccatga ccttcgctca cccttaagta
     4201 ccatcagggg gatgaccgag ctgctttctt caaaattttc caggcagctt ggtgaaaagg
     4261 gggaagagct tatgaactat atcaccagag gcgtgggccg aatgactcag cttctggagg
     4321 acctcctttc ttttgctcgc gcaagccact tcgacgcagc ctccgccaag cccttgccgc
     4381 tcaatgacgt actgacggtg tcactctcga atttgagtgg cgagatagca gaatcggcgg
     4441 cggccgtcac ggcggatcct ttgcccatag tcgcggcaca tgaaacgcac atggtgcagc
     4501 ttcttcagaa tctcattgga aacgccctga aataccgcag ccagcatccc cgcattcaca
     4561 tctcggttga ccagggagat ggcgaatgga tcattggcgt aacggataac gggattggaa
     4621 tagatcccat tcatgctcag cagatttttc agccttttaa gcggttgcac ggagaagaat
     4681 attctggaag cggcattggt ctcgcaagct gccaaaaaat tgtagagggt tacggaggtc
     4741 ggatctgggt cgaagctgag ctgggcaggg gctcgaaatt tttcttcaca ttacccgtta
     4801 ctggagcaaa agcactcccc cagtaaaaga ggcttccagc ttacgcttgt ccaaaaccat
     4861 ttgtgccctt tccaaagaac gatagaaatg gcaaacatcg aactgcctct tgtactggat
     4921 cgcattgaga tgcgccctga cgcgagggcg tgattgccac agccacttcg ggcgtcccgt
     4981 ttaaatcccg gaacgacatc tctgcatgat cgccttcctt aatcattcta aaacgacaaa
     5041 cacgagtgcg cttcattgcg cttaccccgg gaggtagcgt tttgagcggg tcaacggggg
     5101 agagaatgag gagttctggc catctgtgta ggcttgtgag cagcgatctg ccgcaaccgt
     5161 ttgaggtcgt tgaccttgat atgcctgcaa tgcttccgct tctttcccgc actcttcccc
     5221 cgctcttccg gctccagcac gagccggtca ggtctttgta gctatatcag ccagtagacg
     5281 aagatgaaaa gtcccagcca taccacgtcc acaaagtgcc agtaccatgc cactccctca
     5341 aatccgaaat gattctcggg cttgaaatgc ccggcgagaa cccggcagaa aattaccagc
     5401 agcatgatgg agccgatggt tacatggaag ccgtgaaatc ccgtcaacat gaaaaaggaa
     5461 gcgccgtacg ctccgctggt gagcttgagg ttgagctctg aataggcatg gccatattcg
     5521 taagcctgca agcctacaaa gagaaacccc aaggcaaagg tcaggaacaa tccgagcttg
     5581 agttgcccgc gcttgttgag cttgagggcc cagtgcgccc atgtcaccgt cacaccggag
     5641 gtcagcagta ttaatgtatt gagcgctgga agcccccagg ctcccatagg ggtgaacttt
     5701 tcctcgatcc caggtccgga aactggccat cccccagtga agtctggcca aagcagcttg
     5761 ctctggagat cagccagatc cggaaccgaa agcacacgca tgtagaacag tgcaccgaag
     5821 aaggcggcaa agaacatgac ctctgtgaag atgaaccagc tcatgcccca gcggaacgac
     5881 ttatccacct gttcattgta tttgccgctc tcgctctccc gcgcaacggt gcgaaaccag
     5941 acaaaaatca tgtagaagag tattgcgaac ccgattgcaa gcaaccaata gcctacaggg
     6001 atcttgttta ctgtaaatgc cgcgccaaat cccatgaata agagcgctat cgaccccgtg
     6061 atcggatagg ttgacggcgg tggaacgtaa tagtgaccat ggcctccccc atgatctgtt
     6121 ccatgccctg gtgcgtgtcc tgctgtatgc gctgtttgct ggttcatgac ttatccccgc
     6181 ctatacatgt tggttaatga atatgccctt taatggcaat agacctgcca tagataaatg
     6241 tagtataggc agtgcctgag agaaggccag gagaaaaaac actgttctga ttgctccgtg
     6301 tcttcgctcc atctccatgc cgtcttgcat gctcccgtta ccaaccatat tcatattttt
     6361 tgagatctgt gcgctctctt ccttatattc atctaacgaa cctggttgct gccgattatt
     6421 cccataatcg ctactatacc gggggcgttt caaagctgtg gtacggggcg ctggagacgg
     6481 aaggattcac tcggtaaggt gggcgcagtg ggtgcggcga acaccatgtt cgtgtactgc
     6541 tgccatgatt ttctccagtg gctcttttga aggttgtgat aaaagttaag gattttacgg
     6601 gcaacctgaa aaaatcgaag gtggtcccgt ttccttgcgg gcagaggata accgggaact
     6661 cgccgggaag cggaattccg gttggcggga ccaggttgtg gcgactattt agcggcctca
     6721 ggccgagaca aaatcagaga tagatggagg agacgttacc ggctcaggct gtccaagtat
     6781 tccgccagac aagctgaaaa ccgattgagc ggtgatcaag caaaaaggaa gagagaaaaa
     6841 ggaaggcgtc gaagccgctc actcaggcga aaggggacga gggggcgagg gggcgaagga
     6901 aggctaaggg ggaattaaag gaggattatt ggcgccgcga aatccactct ggacgagagc
     6961 agaacgattt tccgctgacc tgaaaaagat ggggagcgat tgactcgaat gaaaaccgtc
     7021 aatgcaacga gctgttagac gctctgcggc gctttatccc ggtctaccgg gccagtagag
     7081 ccggcagacg tgagctgaat catgcgacca aatttatctg ccacaagcag gcatccttca
     7141 agaaatcggc tcgtattgac agacccggga gaattgtcag tcatccagcg gaaaaaagcc
     7201 gcgagataaa ttgccgcaag accggaatct tcaagcaggc ggggaatatg aggcatcttg
     7261 tgcatcgctg cttcgcgcat ccattcgaca gtacggccaa cccgcatcag ccccgcggct
     7321 tgcgcctgca gatgcccggg ctccagtctt ccgtaaacca tctgccgggt cgttttctga
     7381 taggggtaaa aagcgccgag ccacgtcatg atcaagcggt gcaaacgttc gcgcggcgca
     7441 agataaggaa agtcgcacgc ctgcgcagcc ttgagcatga tcctgtccgc acgttcaaac
     7501 caggcatcga cgatgttatc ctttttttga aaatgggcgc gcaggtcatc aagggtaatc
     7561 cctagtccag tcgcgacatc atgcaaacgc acagcttccc atgatgtccg ctcacccagt
     7621 tcaaccgcag tatcaataat ctgatcaatc gttgccatat tccctccggt tataggtgga
     7681 attttggtag acgagacagc cgagccgatt gcagcacaaa gtgtaagtca cattcatccg
     7741 gttatcaata ccgaaaaccg atcaccctcc gcagtcacgg ctgcgactcc cctcggagcg
     7801 gaacgtcctc ccggccccac ggcaaccagc gcccccttct gacggacgca cctatcgaat
     7861 atcatatcgg gcataattcg taagctcgaa cgccgaatca ggtatatgga gcgcctctac
     7921 ctttccttcg tttcgcccag cgtccgggat ttccaccctt cctatttcgc catggtgatg
     7981 gcaacgggca ttgtatcgat tgccttcgat gcgctggctt tcccaggcat agcaaaaacc
     8041 ttgttcatac tgaacctggt tctatatctc atgttctgct tgatgctggg cgcccggact
     8101 gtgttcttcc cacgcaacct cgtggcggaa cttcgaaccc tgccccggag ctggcttttt
     8161 ctggcattcg tgatcggaac aaacacggta ggaatgcagt tcaccacatt tctgcaagcc
     8221 ggcggcctcg caagcgccct ctggttcgtc gcgctcctgg catggctgat attcctgacc
     8281 gttattgctc tcaatgccgc ggccctccca cgggagagcg tgatgcaatc cgtcaatggc
     8341 gccacccttc ttgtcattgt cagcacagta tccctggcgc tgctcggcgc ccggctgctg
     8401 gacccggaac gaggacatgc accgctcttc ctgggccttt gggcgctatg gacccttggc
     8461 ttcattttgt atctgttcgt tgtctactgg attattcgca ggcttttttt cgactgcttt
     8521 cgaatggaag actggcagcc gccgtactgg atctgtatgg gtgctccggc aatcatcacc
     8581 cttgccggtt ccgaattcgt tatccgtctc ccccgctcac aatggaatgg ccttgaccag
     8641 ctcacgctcg ggatgagttt tttcgcatgg attcttggca cgctctggat accctatctc
     8701 ctggtcatgg atgtgcgaaa acttacccgg atcccggcgc cggtacccct atggataaaa
     8761 attttcccct gggcaagata tgcgttcggc accacccatc acgcttatga acctcctgcc
     8821 tggagcagag tttttcccat gggaatgtac acggcatgca cctttttcct ggcgaaggcg
     8881 accgggtatc gtttgcttga atccgtttcc tcctgttggg gctggtttac gttgctgatc
     8941 tgggccctta cgttggtggg tgcgttccgt tccttcggga aattcatggc aaatcgatga
     9001 gcaactcaaa acccgcattt ggcgtttccc ccttcgttcc atagttctgc ccctacctgg
     9061 cgcccagctt aataccgcga ggcagccttc ccacccgcct gccttgccgc ctctacccgt
     9121 atgctatgct gaaaccaatc tcccgctgga ctgtcttttt tgtatatctt ttgtatagct
     9181 tgaggcaatg aagaaggaag caaaatggac aaaaaatttc aaataaactt ctggtatatc
     9241 attattgcca tactcggcat tctggttgtg cagaacacgt acaaagagta tacaaaggtt
     9301 gagccgattc cctacagcaa gtttcactca ctgctggagc aggacaaaat cgcagaaatt
     9361 gccattaccc aaaaccagat ttatggcacg ctcagggaca agggcccgga tgggctgaag
     9421 gatttcgtta ccacgcgcgt tgaaccggag ctggcggaca agctggacaa gcacaatgta
     9481 acctacaccg gggtggtgca aagcacttgg ctgcgcgaca ttctgtcctg gctgctgccg
     9541 acggctctct ttttcggaat atggatattt ctcatccggc gcatgaatcc cggtggcatg
     9601 acgggtggcc tcatgtcgat cggcaaaagc cgagccaagg ttttcgtaga aaaagaaacc
     9661 aaggtcacgt tcgcagatgt cgcgggtgtg gacgaagcga aagaagaact ggtcgaagtg
     9721 gtcaactttc tcaaggatcc acaagggtat tcgaggcttg gcgggcgcgc gcccaaggga
     9781 gtacttcttg tcggcccgcc cggcacgggg aaaacgttgc tggcgcgtgc agtcgcaggt
     9841 gaggccagcg ttccatttct ctcgatctcg ggatcggaat tcgtggagat gttcgtgggg
     9901 gtaggcgccg caagggtacg agacctgttt gatcaagccc ggcaaatggc tcccgccatc
     9961 atattcatcg atgaactgga ttcgctgggc cgtgcccgcg gcgcatacgc gatgggcggg
    10021 catgatgaaa aagaacagac gctgaaccag cttctggctg aactggacgg gttcgacccc
    10081 acgactggtg ttgtcctgct cggagcgacc aaccgacccg aaattctcga tcctgcgctg
    10141 ctgcgcgcgg gcaggttcga tcgccaggtg ctcgtggatc gcccggataa gctggggcgt
    10201 gagcaaattc ttgaagtgca cctgaaaaag ataacgctta gccccgacgt caaaccggag
    10261 cagattgccg cgctgacacc gggcttcaca ggtgcagatc ttgcaaatct ggtcaatgaa
    10321 gctgcgctgc ttgctacccg gcgccgcggt gatgcagtga ccatggacga cttcaataat
    10381 gccgttgagc gcatcattgc aggtctggag aagcgcaacc gcctgctcaa cccctcggaa
    10441 cggcgggttg tcgcctttca cgagttgggg catgccatgg ttgcccttgc cttgccgggc
    10501 actgacgaag tgcacaaggt atcgatcatt ccgcggggtg tgggtgctct gggctacacc
    10561 atccagcgtc ccaccgcaga ccgctttctc atgaagcggg aagaactgga aaaccggatg
    10621 gcggtgatga tgggcggacg ggcggcggaa caggtcgtat tcaatgaagt atctacggga
    10681 gcaacggacg acctggctcg agccactgac ctggcgcgct cgatggtgct gcgctacgga
    10741 atgagcgatg cattgggaaa cgtcgcttac gaccgcgagc gttccgcctt cctccagccc
    10801 ggcatcccgg tagtgcagaa tagggattac agcgaaaaaa ccgcaaatgc ggttgacagc
    10861 gaagtccgca cgctcgtcga ccatgcgctg gaacgggcta tcggcatcct gaaaatcaat
    10921 cgcgcattgc ttgaccggac agcggaagag ttgctcaaga ccgaaactct taatacgccc
    10981 cagatcgagg tactgaagcg tcaaattatt tcaggacccg ccctgcctgc ggaaacggcg
    11041 acagcagccg tggcatagcg gtgtctcaca aagcggaaag aaaggatttg agtctgctcg
    11101 cgcaggcgtg gcctatcgcc gtcctgtgga aaactttcac catataatcg tttcaaaaaa
    11161 gccaaaactc gtgtagaatg tctgtctcga tgagaagcgg ttcctttcgt ttttctcgac
    11221 ccatcgtttc tgactccctt cgatttcaat cttcagcgaa attgtgcggt accttggtta
    11281 gcggcgatgt ttaccagcgc gccagcggta tttgccctat attttcggct gcctgttagg
    11341 cttgcgcatt ttaggcgctt ccagcttcaa agtttcagag gagggcaggc tcgtatcggt
    11401 gctgcttgtg cagtgccatg ccgtacgggt ttcgttgccg gatcacgtgc agacgctatc
    11461 ggacgcaaca accgacaagc tcaggcattc gatatctccg gtattcgatt cctttaagcc
    11521 catctgaaac tcgaacttgc gccccacatg ttcttattga taggaaaaat cacttgtcat
    11581 tcacagacct gaacctgcac ccacttattc tcaaagccat tgccgactct ggctatacct
    11641 cgcccactcc catccaggaa aaggcgattc ccgaactgct cgcgggccac gatgtcctgg
    11701 cctccgccca gacgggtacc ggcaaaaccg ccgcattcat gcttccggcg ctgcaccggc
    11761 tcgccgcgcc atcgactgcg cccggccgcg gcccccgtgt gctggtgctg accccgacgc
    11821 gggaactcgc gcttcaggtt tccgaggccg cggccaaata tggcaaatac ctttcccgtg
    11881 tgagagttgt gagcatactg gggggaatgc cttatccggt ccagaacagg ctgctttcgc
    11941 aaccagtgga aattctcgtg gcaactcctg gccgtttgat cgaccacatc cagcggggac
    12001 gcatcgattt ttcgcgcctc gaaatgctcg tgctggatga agccgaccgc atgctggaca
    12061 tgggttttat tgatgatgtg gaaaaaattg cgtcagccac tcctgccagt cgtcagacgt
    12121 tgctattttc cgctacgctc gatacggcga ttgccgatgt cgctgcgcgc ctgctgaaat
    12181 tgccgaagcg gattcaagtt gccacccaac aagccaagct cgataacatc gaacaacggc
    12241 tgcactatgt cgacgatatt tcgcataaaa acaggctgct ggatcatctg ctgcgcgacg
    12301 cggagctgag gcacgctatc gtttttacgg ccaccaagcg ggacgctgac gtgctggccg
    12361 ataatctcgc cgcgcagggg cacgaggccg ccgcgttgca cggtgatatg agccagcgcg
    12421 accgcacccg gacgcttacg aagctacggc ggggtgggtt gcgcgtgctg gttgcgaccg
    12481 atgtagcggc acgaggaata gacgtggcgg gtatcactca cgtcatcaat ttcgatctgc
    12541 cgaaatttgc cgaggattat gtgcaccgca ttggacgcac gggtcgggcc ggtgcatcgg
    12601 gaattgccgt atcattcgcc tcgggcaagg atgggatcag cttgaaaaag attgagcgct
    12661 tcaccgggca gcggattatg tcacacgtaa ttccgggcct ggaacctcga gccaagccgc
    12721 gttcgagcac ggtcaatacc acccgaagtg caccggctat cgcccttaaa cgcagccgta
    12781 gctggacaaa tgataacgtg agcaggtccg gtggcgcggc ttcgccccgg gaagacgcgc
    12841 gcccccggtc gtcgagggat agccagacca cgtttgggaa tacgcagcgc agcttcagga
    12901 acggtggcga tttcgacgcc ttcagctatc cgcattccag aacgaagcga actccttcgt
    12961 gaagtaatag ctgccgcttc aaagattcgg ctaacgccgg ttgcaccgca gaaagattcg
    13021 agacaagacc gccaccagga tgctcctgct ggcggtcttg ccgtgattga gggcaagcga
    13081 cttatgctcg catgcgcgtt ccgaatttat ttcccgttct gaatttactt tcctttccag
    13141 acttatttcc tactcctcag gctggttccg gatgaaatcc gcggtctgat aaaacgccat
    13201 ggaaagcgtc tttgtaagct gcgcatcgag tccggcatcg cccatcgccc ggctcatgca
    13261 ggacagccac tgatcgcgct cggggatacc aattgaaaat ggcatgtgac gcgtccgcat
    13321 gcgcggatct ccgaatttag cggtatagag cgagggacct cctgtccatc ccgagaggaa
    13381 catgaacagc ttctgccggg agcttgccag gtcctgcgga tggagttttc gtatgccgta
    13441 atagtccgga tcttcgtcca tgagatcata aaagcgatcc acaaggcgac gaatggcatc
    13501 ctcgccgccc atcaattcat aagcctctat aagatgcatg taattctcca gataatgtaa
    13561 tcggcttccg agtcttcagc atctgcagaa tcgagtagac cgcgattgct gcgagcacgt
    13621 gcttcgccgt atgcccgctg atcaagtgcc ctaaatcgta tatttcacgg tcccccactt
    13681 cggccaactt ggcgacagca taccaggcga gtgcaacata aatcccggat gcccgggaat
    13741 atcgcgatgg gaaaaagatt gcgagcagaa caatgaccaa aagcgaatag aactgtacaa
    13801 caatataaaa gttcagattg cccgccccca cctgctcgct ccagtgccaa tacagtacgc
    13861 tcaccatgcc gattccaacc aggataggca aaactcgtaa accgattcgc ggactgaccc
    13921 gctccgtcaa ctgggccgaa agcaaagccg tcattgtcat ggccagtggc aaccggtccc
    13981 acgtcagccg cgcggtgtca ggagccaggt gatagtaggc tgaacccatc cccgtcagtg
    14041 ttatgctcag gaacagaatg aggtaaggcc agcgttcccg gtactcaata aagaggtggc
    14101 cacccttctc tttacttacg gatgccagaa atacaaggcc gtctattcca acaaggagaa
    14161 agccaagatt ggaaatgacg ttccaaaaat ttggaatacc gaaaaagctg cgggtatcgg
    14221 caaactgatg gtacttctca ggctgcggga tgggaggagc aatcgccgcg gcgagcagca
    14281 caacaaggga aacgattgcg agaatgcgca gtctctgtgg gttgctcatg aactgacgtc
    14341 gagaatctgc tggatcttta acgagtcttg cctacggcct cgtccacacg ttccaccccc
    14401 acaatttcaa ttcctgggat tgcctgctta ggcttgtttg ccttggggac aatagcctgg
    14461 acaaaaccga gtttcgcggc ttccttcaac cgttcctgcc cgcgctggac ggggcgcact
    14521 tctcctgcca ggccaacttc cccgaatacc accgtttttt ccggcaacgg cttattcctg
    14581 agcgaggaga caatcgccag cagcacggca aggtcggcgc caggctcggt gattttcacg
    14641 ccccccaccg cattgatgaa cacatcctga tcaaaacaag cgacgcccgc atgccggtgt
    14701 aacactgcaa gcagcatggc aagcctgttt tgctccagac ccacgctcag ccgtcgtgga
    14761 ttgggggcgt gggcttcgtc aaccagagcc tgtatttcga cgagcaatgg acgcgttcct
    14821 tcctgggtta ccatcacaca ggagcccggc acctgcccgc catgatgcga caggaacagg
    14881 gcagatggat tagccacctc tcgcaagcct ttctcggtca tggcaaatac gccgagttca
    14941 tttaccgctc caaaacggtt cttgaaagct cgaaccaggc gaaaactcga atgcgtgtcg
    15001 ccctcgaaat agagtaccgt atccaccatg tgctccaaaa cgcgcggacc agcgagtgag
    15061 ccttccttcg tgacgtggcc gacaaggatg atgcaagtgc cgctggattt cgccagacgc
    15121 gtcaactggg cagcgcactc gcgcacttgt gcgaccgagc cgggcgccga ttgaagcgct
    15181 tccgaataaa tcgtctggat ggaatcgata acggctatgt cgggcttgcg ttccaacatt
    15241 tctgcctgga tactttccag gcggatttca gccagcaaat gaactgcctt tgcatccacg
    15301 cccatgcgct ttgcgcgcaa ggcgacctga cggggtgact cttccccgct cacatacagc
    15361 acatcgcgag cgggcgtgtt attcgttccg agagacatgt ggcaaagcgc ctgcagcagc
    15421 aaggtcgact tgccgattcc cggatcgcct cccagcagta tcactgcccc atgcaccaac
    15481 cccccgccca ggacacgatc aaattcggct atcccggtgg aagcacgcag ctcttccccc
    15541 gcttccacct cgttcagatt ctgtacgcgc ccgctttccg aaatcggaat gaagcgggat
    15601 gcggcttttt ctgcgacaac ctcaaccagc gtattccagg cttgacagtg ggggcactgc
    15661 ccctgccatt tcaatgtctg cccgccgcat cccgagcagg aataggtgga tttggcctta
    15721 accatcctgc cccactgggg tggcatttac gaaatcggca aagccgctga gaaatcgctg
    15781 gagttgaacg cgcacctgct cgtccacgag ttctcccttc tcatcgaaca tattgtttac
    15841 ccgggatacc atcattcgtc cgccaaacca gggacgggta cccaacgttc gcaaaacagg
    15901 cagccag
//
LOCUS       DASSHJ010000253         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_60425, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000253 DASSHJ010000000
VERSION     DASSHJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60425"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFI43_09780"
     CDS             complement(<1..523)
                     /locus_tag="VFI43_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HET7062455.1"
                     /translation="MKIGRGTGVALILLTAATVSAIAMWRHHLKNELPIKVGILQALS
                     GPTVESERPLLEALQLAIEDANAAGGIRGQKIQAVMADCGLDAAQCAREAERLITEEQ
                     VQALFGCWTSGCRKAVKEVVEKRHSLLFYALRYEGMEQSPNILYGGAVPNQLVMPAVH
                     WALVNLGNRSEDPG"
     gene            complement(690..1622)
                     /locus_tag="VFI43_09785"
     CDS             complement(690..1622)
                     /locus_tag="VFI43_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET7062456.1"
                     /translation="MTTAFISHPDCLLHGMGSFHPERPERLKAVEDELQARGVMERLA
                     QHSAPLATFEQLTRVHSPEYVRFVNAAAGRASSSGLAYLDPDTAMNGHSLKAARRAAG
                     AVILAADLVMSKEVENVFCNIRPPGHHAERDRAMGFCLFNNIAAGVAHAMETYGLERV
                     GVVDFDVHHGNGTEDIFRGDPRVMMVSTFQHPFYPYSGVTGRSERMINIPLPAGSNGT
                     VFREAVERYWIPALENFEPQMLFVSAGFDAHADDELASLNLVENDYAWVTERIMEIAL
                     QYADGRIVSALEGGYDLPALGRSVAAHLSVLSDG"
     gene            1820..3781
                     /locus_tag="VFI43_09790"
     CDS             1820..3781
                     /locus_tag="VFI43_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7062457.1"
                     /translation="MPLFRQLWLAVILITLTSFIGSFAISILGTRTYLEQRLQRKNSD
                     TASILARSISQLNKDPVTIGVQVGALFESGQFDRISITAPDGKLIVERVSEQEEKKVP
                     EWFVDLFPISAEAGQAQISDNRMHFGIVKVASHDRLAEQALWEESQALVIWYMMAAII
                     SALIGIFITHAVRRPLATMTEQAEAITERHFLTIAEPGIRELRTIARATNDLVRRLHN
                     RAIEETLHLEALNKRMNHDPLTGLPNRGYFLDKLQAVVDGDQQEDDPELTEAASERPE
                     RGGFLLLLRIENLEEVNRKLGRSTVNSLLRHVAAVVENTGSDNSERIIGRLNGTDFVL
                     ALPGAEEVSDILNGFATTMTTLLPRINSEPRPSWHLGAAQYKDGDKPLDILASADTAL
                     ASAQGKGPNTWQILEKASGGVLAGASGISDWRQIFSSVIAENRFRLALFPVVNAGGAP
                     LHEEALARIQALPNGGWLIAGDFIPIAVRLGVVGTLDLAVVRRAMDYLRSSSGDVAIN
                     LSIETIHDWGSRNQLAELIRQQPDLLRRRLWIEVPESGAHRKIESFRDFCKTFRELGC
                     RIGVEHFGRHSAELQKYADIGLDYVKVDTFFVRGIHQNKENQKLLKFLCGLARTLGIL
                     VIALGVETEAERRALLKIGVDGVTGPYIK"
     gene            3973..>4307
                     /locus_tag="VFI43_09795"
     CDS             3973..>4307
                     /locus_tag="VFI43_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062458.1"
                     /translation="MNSSHTWNFFRAGGFDQVQIDDGADLLALKNLDQKLWVALSCPT
                     RGIEFDSRTLDLIDSDGDARVHANEVLGAIAWAADLLKNPDLLVNGSHRLALAQINDG
                     TDEGQDLIAS"
BASE COUNT          998 a         1235 c         1116 g          958 t
ORIGIN      
        1 cgcccggatc ctcactcctg ttgccaagat ttaccagggc ccagtgaacc gcgggcatga
       61 cgagctggtt cggaacagca cctccataaa gaatattggg agattgctcc attccctcat
      121 aacgcagtgc atagaagagc aggctatggc gcttctctac cacctctttt actgctttgc
      181 ggcagccaga tgtccagcat ccgaacagtg cctgcacctg ttcctctgta atcaagcgct
      241 ccgcctcgcg agcgcattgc gccgcgtcga gcccgcaatc cgccatgacc gcctgaatct
      301 tctggcccct tatgccgcct gctgcattcg cgtcctcgat tgcaagctgc agcgcctcta
      361 ggagcggccg ctcgctttca acagtaggcc ccgataaggc ctgcagtatt cccaccttta
      421 tgggcagttc gtttttcaaa tggtgacgcc acatcgcgat tgccgaaacc gttgcagcgg
      481 ttagcaggat taaggcgaca cctgtgccac gaccgatttt caatgcttaa attcccctgg
      541 ctatcctctt tttttgattc atacgctaca gcttttataa tagacacaat ccgcgctcga
      601 tgaagcagtt gcccttcagg cgcggttatt gctggagtta ttgccgctca ctcgttccag
      661 tacccattct ctgcaataat caataatccc tagccatcgc tgagaacgct caaatgcgca
      721 gcgacgcttc gaccgagggc cggaaggtcg tatccgcctt caagcgcgga cacgatcctg
      781 ccgtcagcat attgcaaggc aatctccatg attctttcag tcacccaggc gtagtcattt
      841 tccaccagat tcaacgaagc aagctcatca tccgcgtgtg cgtcaaagcc ggcggagaca
      901 aacagcatct ggggctcgaa attctcaagg gcgggtatcc agtatcgctc taccgcttct
      961 cgaaagaccg taccattgct gcctgcgggc agaggaatat tgatcattcg ctccgaacgt
     1021 cccgtgaccc cgctgtaggg atagaaagga tgctgaaagg tggaaaccat catcacccgt
     1081 gggtccccgc ggaaaatatc ctcggtgcca ttgccgtgat gaacatcgaa atccaccacc
     1141 ccgacgcgct ccagcccata cgtttccatg gcatgcgcca cacctgccgc gatattgttg
     1201 aaaaggcaga aacccatcgc cctgtcccgt tctgcatgat gaccaggggg gcgaatattg
     1261 caaaaaacat tctctacttc tttactcatt acaaggtcgg ctgcaaggat aaccgccccc
     1321 gccgcacgcc gcgcggcttt caggctatgc ccgttcattg ccgtatcagg atcgagatac
     1381 gccaacccgg atgaagatgc gcgacccgcc gcggcattca caaaacgaac atattccggg
     1441 gaatgcacgc gggtcagttg ctcgaatgtc gccaatggcg cgctatgctg cgcgagccgc
     1501 tccatcacgc cgcgggcttg cagttcatcc tcgaccgcct tcagccgctc cggacgctcc
     1561 ggatgaaatg aacccatgcc gtgcaacaag cagtcgggat gcgaaataaa cgctgttgtc
     1621 atagcctgcc tcctgccgga gaaggaatgg tatcctaaac atccgttgat aataccttcc
     1681 gccaactggc ggtttgggct tccgctcgcc gtccacccat tgattcaagg ataaattgaa
     1741 cccctacctc gtccgtggta acctcacaat aaagagacct cataaaacat tcattccact
     1801 gccagaggtt cgtatttaga tgccactttt tagacaactc tggctggcag tcatcctgat
     1861 tacgcttaca agtttcatag ggagctttgc cataagcatt cttggcacgc gcacctatct
     1921 cgaacaaaga ctccagcgta aaaattccga tacagcgagc attctcgccc gctccatatc
     1981 ccagctcaac aaagatccag tcaccattgg cgtgcaggtt ggcgcgctct tcgagagcgg
     2041 ccagttcgac aggatttcaa tcacggcccc ggacggcaag cttattgttg aacgggtaag
     2101 cgagcaggag gaaaagaagg ttccggagtg gtttgtcgac ctctttccaa taagcgcgga
     2161 agctggacaa gctcagatat cggacaaccg gatgcatttt ggcatcgtga aagtcgcaag
     2221 ccacgatcgc ctggccgagc aggcgctctg ggaggaatcc caggcgttag tcatatggta
     2281 catgatggca gccatcataa gcgcgctcat cggcatattc attacgcatg cggtgaggcg
     2341 acctctcgcg acaatgactg agcaggccga ggcaattacc gaacggcact tccttaccat
     2401 cgccgagccc ggcatccgcg aactccgcac cattgcgcgc gccaccaatg acttggttag
     2461 aaggctgcat aacagggcta tcgaggaaac gcttcacctg gaggccttga ataaacgaat
     2521 gaatcacgat cctctgacgg gtctccccaa ccgcggctat ttcctggaca agttacaagc
     2581 ggtggtggat ggcgatcagc aggaagatga cccggagctg acggaagcag ccagcgagag
     2641 gccggagagg gggggattcc tgcttctcct gcgcatcgaa aatctcgaag aagtcaacag
     2701 aaagttgggt cgcagcacgg taaacagctt gttgcgtcat gtagcggcgg tagtggaaaa
     2761 cacaggcagt gacaattccg aacgaataat cgggcgcctg aatgggactg atttcgtgct
     2821 ggcattgcct ggcgccgaag aggtttcgga cattctgaat ggtttcgcca caaccatgac
     2881 tacgttatta ccgcggatta atagcgaacc ccggccatcc tggcaccttg gcgcagcgca
     2941 gtacaaggac ggagacaaac cgctcgatat ccttgccagc gccgataccg cgctcgcttc
     3001 agctcaagga aaaggaccaa acacgtggca aattcttgag aaggcatcag gaggtgttct
     3061 ggctggggca tctggaatca gcgactggcg ccagatattt agcagtgtca tcgcggaaaa
     3121 tcgcttcagg ctggccctgt ttcccgttgt taatgctggg ggcgcacctt tgcatgagga
     3181 ggcgctggca cgcatccagg ccttgcctaa cggcggatgg ctgattgctg gcgattttat
     3241 ccctatcgct gttcgccttg gcgtcgttgg aaccttggac ctcgctgttg ttcggcgagc
     3301 catggactat ttgcgttcat ccagcgggga cgttgcaatc aatctctcca ttgaaacgat
     3361 tcatgactgg ggttcccgta atcaactcgc cgaactgatc cggcaacaac ccgatttgct
     3421 caggcgccgc ctatggatcg aggtgccgga atccggtgct catcggaaaa tcgaatcttt
     3481 ccgggatttt tgcaaaacct tccgcgaact gggttgccgt attggcgtcg agcattttgg
     3541 acgccactcc gcggagcttc agaagtacgc ggatatcggc ctcgactatg tgaaagtgga
     3601 cacgttcttc gtccgtggca tccatcaaaa caaggaaaat cagaaactgc tgaagttcct
     3661 gtgcggcctt gcccgcaccc ttggaattct ggtgattgcg ctgggggtgg aaaccgaggc
     3721 agagcgaagg gctcttctca agattggagt cgacggcgtc acgggccctt acataaaata
     3781 aaccggtttg caaagtttcg agcgatcagc cctccgactc tcaaacccta ttcagattgt
     3841 gctgagtact ccgtccgagg ggacgtcaat gccaaaccca aacgtttcct cctcaaccat
     3901 ttgcaacgca gggatattaa gctataactg attcaagggc tgcttttcca acaacaatac
     3961 taaggattcg tcttgaattc ctctcacacc tggaattttt tccgcgccgg cggattcgat
     4021 caggtgcaga tcgatgatgg cgccgattta ctggccctca aaaacctcga ccagaagttg
     4081 tgggtcgccc tcagttgccc aacccgtgga atagaattcg actcgcggac gctggatttg
     4141 atcgacagcg atggcgatgc gcgtgttcat gcaaacgaag tccttggcgc gatcgcatgg
     4201 gctgctgacc tgctgaaaaa tcccgatttg cttgtgaatg gttcgcatcg cctcgcgctc
     4261 gctcagatta acgacggcac cgacgaaggc caggatctga ttgcttc
//
LOCUS       DASSHJ010000254        13880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1382346,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000254 DASSHJ010000000
VERSION     DASSHJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13880
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382346"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..489
                     /gene="rnpB"
                     /locus_tag="VFI43_09800"
     ncRNA           195..489
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFI43_09800"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            799..1203
                     /locus_tag="VFI43_09805"
     CDS             799..1203
                     /locus_tag="VFI43_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HET7062459.1"
                     /translation="MEMRFAVQLRVQNHSDKAIDYDGIALDLDLRGSRFATGVSNQNG
                     TIPRYGEAIITVPVTVSAIAIIRHVFDFATGDRARADYRLRGRLGAPGFAIGRSFDSE
                     GEIVLPKLPEGNTDSLPPQRVLKPSPVTVNTY"
     gene            complement(1211..1876)
                     /gene="pyrE"
                     /locus_tag="VFI43_09810"
     CDS             complement(1211..1876)
                     /gene="pyrE"
                     /locus_tag="VFI43_09810"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7062460.1"
                     /translation="MSDFRDEFISFAISKNVLCFGEFRTKAGRMSPYFFNAGLFNDGD
                     SLRKLGQFYAKAITAAELQFDTLFGPAYKGIPLVSAIAIAFAEMGQNVPFCFNRKEVK
                     DHGEGGNLIGAPLAGRVLIVDDVISAGTSVRESVHHIHEAGAEPYGVAIALDRMERGN
                     GMLSATQEVQQVYGIKIINIINLDDILSYLRNRKDFARHLHAVEDYRARYGIDECRER
                     DPR"
     gene            2068..2841
                     /locus_tag="VFI43_09815"
     CDS             2068..2841
                     /locus_tag="VFI43_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7062461.1"
                     /translation="MRIITLNMNGIRSAATKGFFQWLPEQAADIVCVQELKAQTCDLS
                     GEISSPAGYHGYFHCAEKRGYSGVGIYSRNKPDAIIEGVGIPEIDAEGRYLRADFGSL
                     SVISIYLPSGSAGPHRQAAKFHFMDQFFPFLGRLGESGREIILCGDWNIAHKEIDLKN
                     WRSNQKNSGFLPEERTWLGRVFDELEFVDVFRRLNQESDQYTWWSNRGQARAKNVGWR
                     IDYQIATSGIAEKAADVSIYKAERFSDHAPLIIDYDHTL"
     gene            2911..4275
                     /locus_tag="VFI43_09820"
     CDS             2911..4275
                     /locus_tag="VFI43_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7062462.1"
                     /translation="MSSSFPSGWLEAFRIYTHPRVLGMLSLGFSAGLPLLLVLGTLSF
                     WLREAGVDRSTIGHLSWVGLAYGVKWMWSPLVDRLPLPFLTSFLGRRRAWLLLSQVVI
                     ALGLLGMASTDPLDNLAYTVFFALTVAFASATQDIALDAYRIEAVSVRLQGAMAATYQ
                     AGYRLAMILASAGALWIAALVDPSEAAYDYEPWRISYLVMSACMSVGIVTTLIIREPE
                     VPVSQLISENENVARNAIAHWRLNETLRGILAWIYGAVVAPFRDFIVRHGMHALLILA
                     LIAVYRISDVVMGVMSNPFYVDMGYTKEEVATVSKVYGVIMTIAGAAIGGVLTAKLGV
                     MRTLFLGAILAAATNLLFVWLAGRGHDLTGLIFTVSADNLSAGIASSAFIAYLSGLTN
                     SAYSATQYALFSSVMLLLPKFIAGFSGEYVDAYGYPSFFLGTALLGLPVLVLVWLAGR
                     AKFK"
     gene            complement(4268..4906)
                     /gene="metW"
                     /locus_tag="VFI43_09825"
     CDS             complement(4268..4906)
                     /gene="metW"
                     /locus_tag="VFI43_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HET7062463.1"
                     /translation="MPSTGTARPDFALIAEWVKPGAKVLDLGCGDGTLLRFLRDEKGA
                     RGYGVEIDDDNVLACFNNGVNVIQSDLESGLQSFESGSFDYVVLSQTLQAVKNTERIV
                     KEMLRVSREGIVSFPNFGYWKNRIQVMFGHMPVSEALPYQWFDTPNIHNCTLGDFEEF
                     CTQHGAHILERRVMNSNQLVTFMPNLLGMLAFYRFEAQVKRARLTQPLENLT"
     gene            complement(4988..6115)
                     /locus_tag="VFI43_09830"
     CDS             complement(4988..6115)
                     /locus_tag="VFI43_09830"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET7062464.1"
                     /translation="MQDSSSLGPVVPQIARFDTPLHLKSGATVDSYELIYETYGELNA
                     AKTNAVLICHALSGNHHVAGYYDDNPKSAGWWNNFIGPGKSIDTRKYFVIGINNLGGC
                     HGSTGPASIDPKTGKCYGAHFPVVTVEDWVKTQVRLADYLGIEQFAAVGGGSLGGMQA
                     LQWTLDFPDRVRHALIIAAAGKLTAQNIAFNDVARQAIITDPDFHGGDYYSHGVVPRR
                     GLRLARMLGHITYLSDDSMAAKFGRELRNGALAFGFDVEFEIESYLRYQGDKFADQFD
                     ANTYLLMTKALDYFDPAYSHNHDLSAALRAAKADFLVLSFTTDWRFSPERSRAIVRAL
                     LDNELNVSYAEITSSHGHDSFLMEDRHYHKLVRAYMDNIHV"
     gene            complement(6319..8046)
                     /gene="ptsP"
                     /locus_tag="VFI43_09835"
     CDS             complement(6319..8046)
                     /gene="ptsP"
                     /locus_tag="VFI43_09835"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7062465.1"
                     /translation="MSFVLHGVGVFSGIAIGRAHLVSNAALEVAHHTLPADQVSNEIV
                     RLDTAFRVVRAELESLHASVVSGPAAAEYGAFLDLHRMILDDPTLSVAAKAYIAQNHS
                     NAEWAIARQMDVLMAQFEEIEDVYLRERKTDVIQVVERVLKVLLGHPGYVPPALKRDG
                     DSILVAHDLSPADVIQYRQHAFTAFLTDLGGLTSHTAIVARSMNIPSIVALHHARRLI
                     RDGDILIVDGTQGVVVVDPDEHALSEYRLRQSQLELEKQKLKLIKTTVAATLDGTIID
                     LYANIELPSEVEQVKENGAAGIGLFRSEFLFLNRNSLPGEDEQFEAYRTVARKMRGLP
                     VTIRTFDLGADKNLDSAKRVAANPALGLRAIRLCLAEPQMFHTQLRAILRASRYGQIR
                     ILVPMLSSVPEITQTLHLIENAKQSLRLERIPFDENIQIGGMIEIPAAALSVEIFMRK
                     LDFLSIGTNDLIQYTLAIDRADDSVAHLYDSLHPAVLRLVAHIIRSANRTGIPVTVCG
                     EIAGDIIFTRLLLGFGLRLFSMHPVQLLTVKREVLRSSLHQIMPLTRKILKSDDPDRI
                     HSLLAKLNS"
     gene            complement(8043..8312)
                     /locus_tag="VFI43_09840"
     CDS             complement(8043..8312)
                     /locus_tag="VFI43_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET7062466.1"
                     /translation="MQQREVTIRNKLGLHARASAKLTNLAGQFKSEVWITRNSRRVNA
                     KSIMGVMMLAASQGVTLAIETAGKDEIEAMEALVKLIDGRFGENE"
     gene            complement(8315..8704)
                     /locus_tag="VFI43_09845"
     CDS             complement(8315..8704)
                     /locus_tag="VFI43_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET7062467.1"
                     /translation="MIGILIISHGNLGDSLIRCANHVMPEKPERLASLSITVRDDPDV
                     SVRDARVLIEQLDQGEGVLILCDICGATPCNVASRLISPGKVECLAGVNLPMLVRALT
                     YRREALAVTLDKAFNGGRDGVVRLLAG"
     gene            complement(8737..9687)
                     /gene="gshB"
                     /locus_tag="VFI43_09850"
     CDS             complement(8737..9687)
                     /gene="gshB"
                     /locus_tag="VFI43_09850"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET7062468.1"
                     /translation="MKLAFILDQLDSIKTYKDSSFAMMREAVLRGHQLFSMQQGDLVW
                     KDGRTTGVVRALDLTGEGDGAHQWYRAGEQEEIPLTEFDAVMMRKDPPFDMEYVYSTY
                     LLELAESAGARVINSPRAIRDHNEKLAIAKYTEYVPPTLVTRQEHLILDFLAQHGDIV
                     MKPLDGMGGASIFRIQHGGHNIGVIIETLTHYGTRTVMAQRYIPEITQGDKRILLIAG
                     KPVPYSLARIPRQGELRGNLTAGATGVAQLLTSRDREIAEALGPSLQNAGLMLVGLDV
                     IGDYLTEINVTSPTCMREITDQTGFNVAGMMLDALEQTSS"
     gene            complement(9684..10973)
                     /gene="gshA"
                     /locus_tag="VFI43_09855"
     CDS             complement(9684..10973)
                     /gene="gshA"
                     /locus_tag="VFI43_09855"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7062469.1"
                     /translation="MPVPLLATASFGQTPDLERCIVDAMPSIEHWLRNQWQAHEVPFY
                     CSVDLRNSGFKLAPVDTNLFPAGFNNLNPEFMPLCVQAMMAAVEKICADAKNVLLIPE
                     SHTRNIFYFQNIATLQAIMGHAGLDVRIGSLLPEISTATTIALPGGAKVVMEPIRRKG
                     NRLVLDGFDPCVVLLNNDLSTGAPSILENLEQTVIPPLRAGWATRRKSQHFSAYDNVA
                     GQFAGLIGIDSWLINPFFASCGKINFREKKGEDCVASTVDEILREIQEKYDQYGVKDD
                     PFVIVKADAGTYGMGIMTVKDGSEVRALNRRQRNKMAVLKGGLPVTEVLVQEGVYTFE
                     SINEAVAEPVIYMIDHYVVGGFYRIHTSRGIDENLNAAGMQFVPLAFEDTCLLPDRDA
                     KPGSSANRFYSYGVIARLALLAAAQELEKSSERQRAL"
     gene            complement(11218..12771)
                     /locus_tag="VFI43_09860"
     CDS             complement(11218..12771)
                     /locus_tag="VFI43_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7062470.1"
                     /translation="MSLPATEFFYPGANRGEILDSIVYVLTHGEGAEGIIVLTGEEGS
                     GKSTLCRLAMKRLPVQMQAIYIAEPPVDAAEFFSEIARGLTPGVIQPEMNTACESGVP
                     AIASLENLLDQKRAAGVQVVLLLDEAQRLTAEMLDGAGTLYEHASSRQKLLQIVFAGQ
                     NELHRTLALPSMRKVKSLVTHRFHLNPLEPGQVREYLESRIPAQAGDVAGGMFASGAV
                     TPGAVKAIGIASGGLIRTLNQLADKSYSIASPENAEEITEEVARKAIDESGIKYKPGW
                     RNWSKWHNSSRYRSGTALAVSVVMLALLGLLALRPPSAEFNAVAGSASARPVLPLSDS
                     IPANVVIAPAPIPVYPPNLPDSPQIASAASQALTTVSQGQAAREPAVKPRPPEDQAAP
                     IAVQPGTGKLSIGGVKLAGFKLLQQQMESTAKTMETVGDDQYTIQLFSTQNIQPDRME
                     RFLIRAQNIVDLSNLYVHVMKNGDRAKFRVTYGIYSSRDRAAAAMEELPEKYQTEFHP
                     ELFAFADFR"
     regulatory      13033..13122
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      13143..13243
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            13251..>13880
                     /locus_tag="VFI43_09865"
     CDS             13251..>13880
                     /locus_tag="VFI43_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HET7062471.1"
                     /translation="MASPFFLRYQFVKTTALNQIHRDLQAKMVDFGGWDMPLHYGSQL
                     DEHHKVRRDAGMFDVSHMLPIDIKGDGARAFLRRLVANNVDKLVQPGKALYSCMLNPD
                     GGVIDDLIIYYLSESYFRLVVNAGTAEKDMAWLVAKRDEFAPGVEILPRRDLSMIAVQ
                     GPNARAKVWQVIEGSQAVTEGLKLFQAAILDNYFIARTGYTGEDGFEIML"
BASE COUNT         3089 a         3805 c         3769 g         3217 t
ORIGIN      
        1 gagaattcaa ccccggcaat ggatcactaa cagtaacatc cggagcagct ccataagggc
       61 tttaaactcc atcaaacctc aaaagcggct tcaacaaacg ttgaagccgc tttttttcgg
      121 acttccagca gccgcgcttc ccgaaccctc ccatcggatt cgctccggcc atgggttaat
      181 gatatgatgt acatgaagct ggccaggcaa tcgccgcctg ccttgatatg cagggggagg
      241 aaagtccggg ctccatagag cgggatgccg gttaacggcc ggccgccgag aggcgaggaa
      301 cagggccaca gagacgagcg tattaagtta cggtgaaacg cggtaacctc catccggagc
      361 aagaccaaat aggcaggcta tgaagcggct cgctgagcct gcgggtaggt cgcttgagcg
      421 cacgggcaac cgtacgccta gaggaatgat tgcccatgac agaacccggc ttatcggcca
      481 gcttctcctt cgcttcatgt ttccatgtat tgcacctttt cagcagaact cctcgacagc
      541 gttacctgaa gtacgtagtc agctttaccc gactcgccag tcttggccac aatcagtctt
      601 ggccacccta tcctggaaaa gagctccccg ctgtgattca acccgaagtc ctggcgttca
      661 ccaaaggggt ctagcgtgac ctttcgccta tttcttatct ctggattgct gtatatcctt
      721 tccggctgtg cgagcttcac cttacaggat ccgctgcgga tttccgtagc tggaatggag
      781 ccgcttgagg gacaaggaat ggaaatgagg ttcgcggttc agctcagagt tcagaaccat
      841 agcgacaagg cgatcgatta tgacgggatt gctcttgacc tggacttgcg cggctcgcgt
      901 tttgccactg gggtaagcaa tcagaacggc accatccctc gctatggcga agccatcatc
      961 acggtgccgg tcacggtttc tgcgatagca atcattcgcc acgtattcga cttcgcgacc
     1021 ggggatcgcg cccgggctga ttaccggttg cgcggacggc tcggcgcgcc aggtttcgcg
     1081 atcggtcgaa gctttgattc ggagggagaa atcgtgctgc ccaagttgcc ggagggcaac
     1141 acggattccc tgccacctca gcgcgtcctt aaacctagtc cggtaaccgt aaacacgtac
     1201 taaccctgcg ctacctgggg tccctttcgc ggcattcgtc tatcccgtac cgggcgcggt
     1261 aatcttcaac cgcatgcaga tgacgagcaa aatccttcct gttccgcaga tagctgagga
     1321 tatcgtcgag attgatgata ttgatgattt ttatcccata cacctgctga acctcttgcg
     1381 tggccgagag cattccgttt ccccgttcca tgcggtcaag ggcgatagca actccgtaag
     1441 gttcggcccc agcttcatgg atatggtgga ccgattccct taccgatgtg ccggccgaaa
     1501 taacgtcatc aacgatcagt acacgcccgg cgagtggcgc gccgatgagg tttccgcctt
     1561 caccatgatc ctttacctcc ttccggttga aacagaaagg gacattttgc cccatctcgg
     1621 caaaggcgat ggcgatggcg cttacaagag ggatgccctt gtaggcgggt ccgaacaggg
     1681 tgtcgaattg aagctcggca gcggtaatgg ctttcgcgta aaattgccca agctttctga
     1741 gagaatctcc atcgttaaaa agcccggcat taaaaaaata aggcgacatt cggccggctt
     1801 tcgtcctgaa ttccccaaag cacagcacgt tcttgctaat cgcgaaactt atgaattcgt
     1861 ccctgaaatc cgacatcgac atatattagg aacctttgaa taggtcctcg tggagcgcgt
     1921 tgctggtaaa atcgggatga tcacaaggcg cgcgacgcag gtcgtagccg ggtctacgat
     1981 gccaaagagc gcaacgaggt gagcgcctga ttttaccgca acccgggtgg gaccacgatg
     2041 acttattcag aggttcctta actgctcatg cgaattataa cgcttaacat gaatgggata
     2101 cggtcagcgg cgaccaaggg tttctttcag tggctgccgg agcaggcggc cgacatcgtt
     2161 tgcgtgcagg aactcaaggc acagacctgc gacctttccg gggaaatatc ctctccggcc
     2221 ggttatcacg gttattttca ctgtgccgag aaaagagggt acagcggggt tggaatctac
     2281 agcaggaaca agccggatgc aatcatcgag ggcgtgggga ttcccgagat agacgccgaa
     2341 gggcgttacc tccgcgctga ctttggcagc cttagcgtaa tatcgatcta ccttccttcg
     2401 ggttcggccg gcccccatcg ccaggctgcc aagtttcatt tcatggatca atttttcccc
     2461 tttcttggaa ggctcggaga atccggccgg gaaattatcc tttgtggcga ctggaatatc
     2521 gcccacaagg aaatcgacct gaaaaactgg cgaagcaacc agaagaattc gggctttctg
     2581 ccggaagaaa ggacctggct tgggcgcgtg tttgacgagc ttgaatttgt ggatgtgttc
     2641 aggcgactca accaggagtc agatcaatat acctggtggt ccaaccgtgg gcaggcacga
     2701 gcaaaaaatg tggggtggcg catcgattac cagatagcga catcgggcat cgccgaaaag
     2761 gccgccgatg tttccatcta taaggcagaa cgcttctcgg atcacgcgcc gttgatcatc
     2821 gattacgacc ataccctatg acttttggtc ggatgagtgc gggcggatca aagcatacgt
     2881 ggaacatccc gtggccgcgg tgttcctagg atgagctctt ctttcccgtc cggctggctt
     2941 gaggcatttc gcatctacac tcatccccgc gtgctcggga tgctgtcgct gggtttctcg
     3001 gccgggctgc ccctgctgct ggtcctgggg accctttcat tctggttgag ggaagcgggg
     3061 gtcgatcgtt ccaccattgg ccatttgagc tgggtcggac tcgcctacgg ggtcaaatgg
     3121 atgtggtctc cactcgtgga tcgcctgccc ctgccctttt taacgtcctt cctcggccgc
     3181 aggcgggcct ggctattgct ttcgcaggtt gtgatcgcgc ttgggctctt agggatggca
     3241 agcactgatc cgctcgataa tcttgcttat acggtttttt tcgcgctgac cgtcgcgttc
     3301 gcttccgcca ctcaggatat tgcgctcgat gcctatcgta tagaagcggt ttcggtgaga
     3361 ctccagggag cgatggcagc aacgtaccag gcgggctatc gtctcgccat gatccttgcc
     3421 tctgccggcg cgttgtggat tgctgccttg gtggatccct cggaggcagc ctatgattat
     3481 gagccgtggc gcatttcata cctggtcatg tcagcctgca tgtccgtcgg tatcgtcaca
     3541 acccttatca ttcgcgagcc ggaagtaccc gtctcccagt tgatatcaga gaacgagaac
     3601 gtcgccagga acgcgatcgc tcactggcgt ctgaatgaaa cgctcagggg gatactggcg
     3661 tggatttatg gagcggtcgt agcgcccttc agggacttta tcgtacgcca tggcatgcat
     3721 gccttgctga ttctggcatt aatcgcagtt taccgaatct cggacgttgt aatgggtgtg
     3781 atgagcaatc ccttttatgt ggatatgggt tatacgaagg aagaggtggc gacggtatcg
     3841 aaggtttatg gagtcatcat gaccattgcc ggggcagcca taggaggcgt tctgactgcc
     3901 aaactcggcg tcatgcgcac gctgtttctc ggagcaatcc ttgcggcggc cacgaacctg
     3961 ttattcgtct ggctcgcggg gcgaggccat gatttgacgg gcctgatatt taccgtttcg
     4021 gccgacaacc tgtccgcggg tattgcgtca tcggctttta tagcctatct atccggcctg
     4081 accaactccg cttattcagc gacgcagtac gcattgttca gctcggtgat gttgctgcta
     4141 cccaagttta ttgcgggctt cagtggtgaa tatgtcgatg cttacggcta tcccagcttt
     4201 tttttgggga ccgcactgct cggcctcccc gtattggtgc tggtatggct ggcggggcga
     4261 gcaaaattca agtgagattt tcaaggggct gagtgagccg agcacgtttg acctgcgcct
     4321 caaatcgata aaaagcaagc atgcccagca ggttgggcat gaacgtaacg agctggttgc
     4381 tgttcattac cctgcgctca agaatgtgcg ccccatgctg agtgcagaat tcctcaaaat
     4441 ctcccaaggt gcagttgtga atgttggggg tatcgaacca ctgatacggc agcgcttccg
     4501 aaacaggcat atgaccgaac atgacctgga tgcgattttt ccagtagccg aaattcggaa
     4561 acgaaacgat tccttccctg ctcacccgca gcatttcctt gacgattctc tccgtgttct
     4621 tgactgcctg cagggtttgg gacaggacga catagtcgaa cgatcccgat tcaaaactct
     4681 gcaggccgga ttccagatca ctctggataa catttacccc gttattgaaa cacgccagaa
     4741 cgttatcatc gtcgatctcc accccgtaac cgcgtgcgcc cttttcatcc ctgaggaagc
     4801 gcagcagggt gccatcgcca cagcccaggt caagtacttt tgcacctggc ttcacccatt
     4861 cagcaatgag cgcaaaatcg gggcgagcag taccggtcga gggcattggg ttgtcaatgt
     4921 cgtctgtggg tgaaaaactg gaagctttgg tcaaggcatc accgcttgca ggagaagcat
     4981 atcccggtta cacatggata ttatccatgt acgcgcgcac gagcttgtga taatggcggt
     5041 cctccatgag gaaggaatcg tgaccatggc tggaagtaat ttccgcataa ctgacgttca
     5101 actcgttgtc gagcaatgcc cggacaatgg ctcttgaccg ctctggagaa aaacgccagt
     5161 ccgtcgtaaa cgagagcacc agaaaatccg ccttggctgc ccgcaacgct gcactgagat
     5221 cgtgattgtg cgaataagcc gggtcgaagt agtccagggc ctttgtcatg aggaggtaag
     5281 tattcgcatc gaactgatcg gcaaacttgt ccccctggta gcgaagatac gactcgattt
     5341 cgaactccac gtcgaaaccg aaggcgagag ccccgttgcg caactcccgc ccgaacttgg
     5401 ccgccattga gtcatccgac aggtaagtga tatggccgag catgcgcgca agccgcaaac
     5461 cgcgacgagg gaccactccg tgagaatagt agtcgccgcc gtgaaaatcg ggatctgtaa
     5521 tgatcgcctg gcgagccaca tcgttaaacg cgatattttg agcggtcagt ttgccggcgg
     5581 ctgcaatgat caatgcatga cgtactctat cgggaaaatc cagcgtccat tgtagcgcct
     5641 gcattccgcc aagacttccc cctcccaccg ctgcaaactg ctcaataccg aggtagtctg
     5701 ccagccgcac ctgcgttttg acccagtctt ccacggtcac aacaggaaaa tgggctccgt
     5761 agcacttgcc cgttttggga tctatgctgg cagggcccgt ggaaccgtgg caacctccca
     5821 ggttattgat gcctatcacg aaatactttc gggtatcgat ggacttgcca ggcccgatga
     5881 aattgttcca ccagcccgcg ctcttcggat tatcgtcgta gtagcccgct acgtgatggt
     5941 tgcctgaaag cgcatggcat ataagaacag cattggtctt tgcagcattg agttcgccgt
     6001 aggtttcata aatcaactcg tagctatcga ccgttgctcc actttttaaa tgcagcggag
     6061 tgtcgaagcg ggcgatttgt ggaacgaccg gaccaaggga actggaatct tgcatcaagg
     6121 aataatgttc acgaaaggct gcaaacaact aatctcaaat cgcctggcaa ttcccgtcga
     6181 gccatcttac ggacttcccc gtaaccacac aggtgccagg cgcaacggtt aaccgcgcat
     6241 tataggcaaa tgcatccctt aaaatccatg gtgggccgta ggtgcagcac gtcgacgtgg
     6301 agctcaaggt aacataactc agctgttcag tttcgcgagc aagctatgga tcctgtccgg
     6361 atcatccgat ttgaggatct ttcgagtgag cggcatgatt tggtgcaagc tgcttcgaag
     6421 tacctctctt tttactgtca gcaactggac cggatgcatg gaaaacaagc gcaacccgaa
     6481 accaagcagg aggcgtgtga atatgatatc cccggcaatc tctccacaca ccgtcaccgg
     6541 tatccctgtg cggttagcgc tacggatgat gtgggcgacc agccggagca cggctggatg
     6601 caaggagtcg tacagatgag caaccgagtc gtcggctcga tcaatcgcaa gcgtatattg
     6661 aatcagatca tttgtgccga tggacaaaaa atccagcttg cgcatgaaga tctctacaga
     6721 aagcgcagca gcagggattt cgatcatgcc tccgatctga atgttttcgt caaacgggat
     6781 tctttccagg cgcaggctct gtttcgcatt ttctataagg tgcaaggtct gagtgatttc
     6841 gggtacgctc gagagcatgg gcaccaggat gcgaatctgc ccgtagcggg aagcgcgcaa
     6901 tattgcgcga agctgggtgt ggaacatttg cggttcggca aggcaaagcc ggatggctcg
     6961 cagtcctagc gcgggatttg cggcgacccg cttggcgctg tccagatttt tatccgctcc
     7021 aagatcaaac gttcgtattg tcacaggcag gccgcgcatt tttctcgcaa ctgtccgata
     7081 agcctcgaac tgttcgtcct ctccgggcaa actgttgcga ttcagaaaga ggaactcgct
     7141 gcgaaacagg ccgatacctg ccgctccgtt ctccttcacc tgctcaactt ccgatggcag
     7201 ctctatattc gcgtagagat cgattatcgt gccgtcgagt gttgccgcaa cagtggtctt
     7261 gatgagcttg agcttttgct tttcgagttc aagctggctt tgccgcaacc ggtattccga
     7321 cagtgcgtgc tcgtccgggt cgacgaccac gacaccttgc gtaccatcca caatcaggat
     7381 atcgccatcg cggatcaggc ggcgcgcatg gtgaagcgcc acgatcgaag ggatattcat
     7441 actgcgcgca acaatcgccg tatgcgaggt gagcccgccc aggtcggtca aaaaggccgt
     7501 gaatgcgtgt tggcgatact gaatgacgtc ggcaggacta agatcgtgcg caacaagaat
     7561 actgtcccca tcccgcttga gcgcgggcgg cacgtagcct ggatgaccga gcagaacttt
     7621 caggacgcgc tctaccacct gaatcacatc ggttttacgt tccctgaggt agacgtcttc
     7681 aatttcttcg aattgggcca tcaggacatc catttgccgg gcgatcgccc actccgcatt
     7741 ggaatggttc tgtgcaatgt aggccttggc ggcgacggaa agcgtgggat catccagaat
     7801 catgcgatgc aaatcaagaa atgcaccata ttcggcagcc gcgggcccgc tcaccaccga
     7861 ggcgtgcaag ctctcaagct ccgcgcgcac aactctgaat gcggtatcga gccggactat
     7921 ttcgttgctg acctggtcag caggtaaagt gtgatgcgcc acttccagtg ccgcattaga
     7981 tacaagatgc gcgcgcccta tggcaattcc gctgaagacg cccacgccat gcaggacgaa
     8041 gctcactcgt tttccccaaa acggccgtcg atcagcttga ccagcgcctc catcgcctcg
     8101 atctcatcct tgccagcggt ttcaatagcc agcgtcaccc cctggctggc ggccagcatc
     8161 atgacaccca ttatgctctt tgcgttgact ctgcgactgt ttcgcgttat ccacacctcg
     8221 cttttgaact gccctgccag attggtaagc ttcgccgagg cccgcgcatg caaacccagt
     8281 ttattccgga tggttacctc tctctgctgc atgatcaccc agccaaaagc cggacgacgc
     8341 cgtccctgcc accgttgaac gccttatcca acgtcactgc aagtgcctct cgacggtaag
     8401 taagcgcccg cacgagcatc gggagattaa cgcctgcgag acattcgact ttgccaggac
     8461 tgatcaggcg gctcgccaca ttgcacggcg ttgccccaca gatatcgcat aagatgagaa
     8521 cgccctctcc ctggtcgagc tgctcgatca acacccttgc gtcgcggact gatacatcag
     8581 ggtcatcccg caccgtgatg ctgagacttg ccagacgctc cggtttttct ggcatgacat
     8641 gattcgcgca gcggataagg ctgtcgccca ggttgccgtg agagataatg agaattccga
     8701 tcattctcgc aaatgccgca ttagaattcg ggggtttcag gaggatgtct gctcaagcgc
     8761 gtccagcatc atcccggcca cattaaaacc ggtttgatcg gtaatttcgc gcatgcaggt
     8821 ggggctggtg acattgattt ccgtcagata atcgcctatc acgtccaggc cgaccagcat
     8881 caagccggca ttttggaggc tggggccaag tgcttcagca atttcccgat cgcgagaagt
     8941 gaggagttgc gcgacacccg tcgcgccagc ggtaaggttg cctcgcaatt cgccctgacg
     9001 gggaattcgg gcaagagagt acggcaccgg ttttccagct atcagcaaaa tgcgcttgtc
     9061 accctgtgtg atttccggga tataccgctg agccattact gtacgagtgc cataatgcgt
     9121 gagggtttca ataatgacac cgatattgtg gccaccatgc tggatgcgga aaatgcttgc
     9181 gcctcccatg ccatccagcg gtttcattac gatatcgcca tgctgcgcaa gaaaatcgag
     9241 gatcaaatgc tcctgccggg taacgagtgt gggtgggaca tattccgtgt actttgcgat
     9301 ggcaagcttt tcattatggt cacgtatcgc gcgcggactg ttgatgacgc gcgcgcccgc
     9361 gctttcagcc aactccagca ggtacgtgga gtagacatac tccatgtcga atggcggatc
     9421 tttccgcatc atcacagcgt caaattcggt taacgggatt tcctcctgct cgcccgcgcg
     9481 ataccactga tgcgccccgt ccccttcccc cgttaaatcc agcgcccgca caaccccggt
     9541 agttcggccg tctttccata cgaggtcacc ctgctgcatg gaaaatagct gatggccacg
     9601 caacacggct tctcgcatca tggcgaaact tgagtcctta taggtcttga ttgaatcgag
     9661 ctggtcgagg ataaaagcga gcttcataac gcacgctggc gctcactgct cttttccagc
     9721 tcttgcgctg ctgccagcag tgccaggcga gcgatgacgc cgtaggaata aaaccggttg
     9781 gcgctcgagc ccggctttgc gtcccggtca ggcagcaggc aggtatcttc gaaagccagc
     9841 ggcacgaact gcatgcccgc agcattcaag ttctcgtcaa tgccgcggct cgtatgtatg
     9901 cggtaaaaac cgcccaccac gtaatggtcg atcatgtata tcactggttc cgccaccgct
     9961 tcattgatgc tttcaaaggt gtaaacaccc tcctggacca gcacttcggt aaccggcaac
    10021 ccccccttca gcaccgccat cttgttgcgc tgcctccggt tgagcgcacg cacctcagag
    10081 ccgtccttga cggtcatgat gcccatcccg taggtgccgg catcggcctt tacgatgacg
    10141 aaaggatcgt ccttgactcc atactgatca tatttttcct ggatctcccg cagaatctcg
    10201 tccacggtgc tcgcaacaca atcctctccc ttcttctccc gaaagttgat ttttccgcac
    10261 gacgcaaaga atgggttgat gagccacgag tcgataccga tcaggcccgc gaactgccct
    10321 gccacgttat cataagccga aaaatgctgg gatttgcgtc gtgtggccca acctgcccgc
    10381 aggggaggta tgacggtctg ttcgagattc tcgagaatcg agggagcccc ggtggagaga
    10441 tcattgttga ggagcactac acaggggtcg aagccgtcca agacaaggcg atttcccttg
    10501 cgcctgatag gctccattac aactttcgcg cctccgggaa gcgcaattgt tgtggctgtc
    10561 gagatctccg ggagcagact gccgatcctt acgtccagcc ctgcatgccc catgattgcc
    10621 tgcagggtcg caatattctg gaaatagaaa atatttctcg tatgactctc cggaatcaac
    10681 agaacgtttt tggcatcagc gcatattttc tcaacggctg ccatcatcgc ctgtacgcaa
    10741 agcggcatga attcgggatt aagattgttg aatcccgcgg gaaacagatt ggtatccacg
    10801 ggagccagct tgaagccgct gttgcgcaaa tcaaccgagc agtaaaaagg aacctcgtgc
    10861 gcttgccatt gattgcgtag ccaatgctcg atcgaaggca tcgcgtctac aatgcatctt
    10921 tcaaggtccg gagtctgtcc gaaggaggcg gtggcaagaa ggggcacagg catcgaattg
    10981 aattaatctt gacggagtat aatcatttta acatcatgga ctactcccct tggcatggga
    11041 agcgtttata tggatcacgg gccgactgaa ggcagggctc ccttgcgatt gctcgagcag
    11101 aagtgcgtgg agctccgcga ttttcaatga ttcaggaggc ctgaataaga attaacgctc
    11161 ctttttagca agggagaagt gaaactgcgc gcaatagcaa aatgccccgc ttcccgttca
    11221 gcgaaaatcc gcaaaggcga aaagctcggg atggaactcg gtttgatatt tttccgggag
    11281 ttcctccatg gcggccgcgg cgcgatcgcg gctggagtat atgccgtagg tcactctgaa
    11341 tttggctcta tctccattct tcatcacatg cacgtagaga ttggataagt cgacaatgtt
    11401 ctgggcgcgg atcagaaatc tttccatccg gtctggttga atgttttgtg tggaaaataa
    11461 ttggatcgta tattggtcat cacccaccgt ctccattgtt tttgctgttg actccatttg
    11521 ttgctggaga agcttgaagc ctgcgagttt cactccaccg atactaagct ttcccgtccc
    11581 gggttgcacc gctatcggag cagcctgatc ctcgggagga cgaggtttta cggcaggttc
    11641 gcgagcagcc tgaccctggg agaccgtggt taaagcctgg gaagcagcgc tggcgatctg
    11701 gggcgagtcc gggagatttg gaggataaac cggtataggt gcgggagcga tcacgacgtt
    11761 tgcagggatc gaatcggata gcggcaaaac cgggcgagcc gatgccgagc cggcaacagc
    11821 gttaaactcg gccgatggtg gccgcagggc gagcaagccc agcagggcga gcatcaccac
    11881 tgaaaccgca agcgccgtcc cgctgcgata gcgtgacgag ttgtgccact tgctccagtt
    11941 ccgccaaccg ggtttatatt ttattccact ctcgtcaata gcctttctag cgacctcctc
    12001 cgttatttcc tccgcatttt cggggctagc aatgctataa gatttatcag ccaactggtt
    12061 gagcgttcgg atcagacctc cagaagcgat gccgatcgct ttgacagccc cgggagtgac
    12121 agccccggaa gcgaacatgc cacccgccac atcccccgcc tgcgcaggga tgcgcgattc
    12181 gagatattcc ctcacctggc cgggttcaag cgggttcaaa tggaatcgat gagtaaccaa
    12241 actcttcacc ttgcgcatcg aaggcaaagc cagggtgcga tggagttcgt tctgtccagc
    12301 aaacacgatt tgcagaagct tttgccggga ggatgcatgt tcatagagtg ttcccgctcc
    12361 gtcaagcatt tcggccgtga gtcgttgcgc ttcatcaagc agtaatacta cctgtacgcc
    12421 tgcggcccgc ttctgatcca gtaaattttc caggctggca atcgctggta cgccgctttc
    12481 gcatgcagta ttcatctctg gctgaatcac tccaggggta agccccctgg cgatttcgct
    12541 aaaaaattcc gctgcgtcta cggggggttc cgcgatgtat atggcttgca tctggacagg
    12601 caagcgtttc atcgcaagac ggcagagggt cgacttgccg ctgccctctt cgccggtcag
    12661 tacgataatt ccctccgcac cttcgccatg cgtgagcacg taaacgatcg agtccagtat
    12721 ctcgccccga ttggctccgg ggtaaaagaa ttcagtagcg ggaaggctca tacgttagat
    12781 gctggaatga ttccgtgttt tggatagatc gcagctggtc ctctggaaag gataatagat
    12841 aataaataga aatatcaccc gcagcgaaaa cgcccccgga ggaaagaata agataacact
    12901 atacctcgca agtttcttcc ccgaaacggt cgcaaatctc gtgtttgaga catcgggcac
    12961 gtgcggttaa cgggatagct tttatgcctt gtattgaacc ctcccggtgg agtatagttc
    13021 gcgtctgcca tttatttcca attggaagag tgcgccgatt ttatcgccgc tgccgaaggc
    13081 gcaacccccc ggaatcgctc aggcgcagat caagcagatc ttgatccgtt gtaaccaatt
    13141 ggaacaggca atctggagag tgccgtgtca acatcggcca ccgaaggggc aacgcggacg
    13201 aacattccgt aatctctcag gtaaaaagga cagaggggtg aggccacatg atggcttcac
    13261 cctttttttt gaggtatcaa tttgtgaaaa ccaccgctct taaccaaatt catcgggact
    13321 tgcaagccaa aatggtcgat tttggcggat gggacatgcc tttgcattat ggctcccagc
    13381 ttgacgaaca tcacaaggtt cgccgcgacg caggcatgtt cgatgtctcc cacatgttgc
    13441 ccatagacat caaaggagat ggcgcgagag cattcctgcg ccgcctggtc gccaacaatg
    13501 tcgataagct cgtccaaccg gggaaggcgt tgtactcctg catgcttaat cccgatgggg
    13561 gcgtaataga cgatctcatc atttattacc tgtcggaatc gtacttccgt ttagtcgtca
    13621 atgcggggac agcggagaag gatatggcgt ggctggtggc aaaacgtgac gagttcgctc
    13681 caggggtgga aattcttccg cgtcgcgatc tttccatgat tgccgtgcaa ggcccaaacg
    13741 cgcgtgccaa ggtgtggcag gtgatcgaag gatcgcaagc cgtgacggaa ggcttgaaac
    13801 ttttccaggc ggcgatcctt gataattatt ttattgcacg tactgggtat accggtgaag
    13861 acggctttga aatcatgctg
//
LOCUS       DASSHJ010000255         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1468152,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000255 DASSHJ010000000
VERSION     DASSHJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468152"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1413)
                     /gene="lysA"
                     /locus_tag="VFI43_09870"
     CDS             complement(91..1413)
                     /gene="lysA"
                     /locus_tag="VFI43_09870"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7062472.1"
                     /translation="MSGDVSASAFAYRDNRLWGESVPLDRIAAAVGTPCYVYSRSTLI
                     AAYEEFDAAFAARDHLICYAVKANSNLSILNLFARLGSGFDIVSGGELQRVLKAGGDP
                     RKIVFSGVGKQAHEMRAALEAGIFCFNVESEAELEVLDRVAGEMNKIAPVSLRVNPDV
                     DAKTHRYISTGLKENKFGIPVDQAESLYASYRQFPNIRMTGLDCHIGSQLTELDPFIE
                     ASGKMLELLDRLEARGLHIEHLDLGGGLGIRYSGETPPVVADYVEALRGAVGQRAQRI
                     VIEPGRALVGNAGLLLSRVEYLKHAPHRNFAIVDAAMNDLMRPALYDAYHEILPVQPR
                     KDGPLENYQVVGPVCETGDFLGHDRRLTLAEGDLLAIMSAGAYGMSMSSNYNTRARAA
                     EVMIDGDRFHVIRQRETMEQLMAGEAIPLWHGEHRSPAASAPATPEKA"
     gene            complement(1410..1541)
                     /locus_tag="VFI43_09875"
     CDS             complement(1410..1541)
                     /locus_tag="VFI43_09875"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HET7062473.1"
                     /translation="MRNYCTVVLIALLLGACGLKAPLYLPQQQGEVQSKQAPQDQKK"
     gene            complement(1616..2269)
                     /locus_tag="VFI43_09880"
     CDS             complement(1616..2269)
                     /locus_tag="VFI43_09880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET7062474.1"
                     /translation="MRINSSGLTVRIPMRESMGWVESVLQKRADWIVKKLEEWKDRKP
                     RGPVWEEGAIFPLLGEPWKLIAAVKGSAHMVRLSENTRVECRQAGLGLPSLLTPRQIE
                     DVVMNWYRSQALACFTERVAYYAPKLGVSVRELKLSSARTLWGSCTAKGVVRLNWRLI
                     QKPLHLIDYVVAHELSHLIEMNHSSDFWRIVERIYPDYVTARRELKSYGQSYGQLQG"
     gene            complement(2413..3135)
                     /locus_tag="VFI43_09885"
     CDS             complement(2413..3135)
                     /locus_tag="VFI43_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7062475.1"
                     /translation="MVLTVLRSAIYVLLQIAITPFYFLIVLAAFPLPPLKRYRITSGW
                     AHFMLYLLRVVCGIDYRVIGGQHIPQTPSIILSKHQSAWETLAFQQIFPPQVWVLKKE
                     LLLVPFFGWGLAMTSPIAIDRSSGKAALKQIVEQGKDRLKKGFWIVIFPEGTRIAPGS
                     KGKYRIGGAWLATHTNVAVVPVAHNAGEYWGKNALIKLPGTITVSIGAPIDPTGMEPD
                     ELNARVESWIEAEVERIGHRKP"
     gene            complement(3138..3671)
                     /gene="gmhB"
                     /locus_tag="VFI43_09890"
     CDS             complement(3138..3671)
                     /gene="gmhB"
                     /locus_tag="VFI43_09890"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HET7062476.1"
                     /translation="MKLAILDRDGVINYDSAAFIKTPDEWKPIPGSLEAIAHLTQSGY
                     RVVVATNQSGISRGLLDMVALNAINDKMCKAVNQVGGRIDAMFYCPHANADKCNCRKP
                     AIGMFKEISERFGLTLRNVPAIGDSLRDLRAAAAVGAIPILVLTGKGEKTRQEGGLPE
                     NTRVFPNLAAAVDALIE"
     gene            complement(3679..>5173)
                     /gene="glyS"
                     /locus_tag="VFI43_09895"
     CDS             complement(3679..>5173)
                     /gene="glyS"
                     /locus_tag="VFI43_09895"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7062477.1"
                     /translation="VIASFKDRRGEIVHQLDDAAARLGLKKAEDDALLDEVTGLVECP
                     NVLAGEFEREFLEVPNECLILTMKANQKYFPLLDVGGKLSNKFLIVSNIHPADASQII
                     SGNERVLRSRLADAQFFFDQDRKKTLASRVEGLREVVYHHKLGTLAERVERIWAIAQA
                     IGLELDGNELAAKAGEAAMLCKADLLTGMVAEFPELQGIMGRYYSLHDGVAEDVADAI
                     EDHYKPRFAGDVLPRNRVGVCIALADRLESLVGMFGIGQIPTGDKDPFALRRHALGLI
                     RILIERELPLALDALLEQVLSIFGNRINNPTPVLTDFIYERIRHLFGEAYQDQSSSGC
                     SQGSDIANSRVQLYSPQQIDAVLALRPRRLHDVKKRLDAVRAFAALPEAESLAATNKR
                     VGNILKKAGVSGEKRVDSKLLQAPAERALYAALARIVPEADADFEAGDYSSSLQILAA
                     LKQPVDEFFDHVMVNTEDETLRNNRLALLATLRVAMNRVADISRLAV"
BASE COUNT         1110 a         1539 c         1347 g         1177 t
ORIGIN      
        1 atcgattcgg ccgcgatccg gcagcgggat ccagcgtgat gattacagcc gtgacggata
       61 gcgggggatt ctttatcttc ctcgggctgg ctacgctttt tctggtgtag cgggggctga
      121 cgccgccggg ctgcggtgct cgccatgcca gaggggaatc gcttccccag ccatgagttg
      181 ttccatcgtt tcccgttgcc ggatcacgtg aaacctgtct ccgtcgatca tcacctcggc
      241 cgcccgggcg cgggtattat agttcgagct catgctcatg ccataagctc ccgccgacat
      301 gatcgcgaga aggtctcctt cggccagcgt cagcctccgg tcatgtccga gaaaatcgcc
      361 cgtttcgcag acggggccga caacctggta gttttctaga gggccatcct tccttggctg
      421 gacaggaaga atctcatgat atgcgtcata aagcgcgggg cgcatcaaat cgttcatggc
      481 cgcatccacg atcgcgaaat tcctgtgggg ggcgtgtttc agatattcca cgcgggagag
      541 cagcaaaccg gcatttccca cgagcgcccg gccgggttca atgacgatgc gttgcgcgcg
      601 ctggccaacc gccccgcgta acgcttcgac atagtctgca actaccggag gggtttctcc
      661 cgaataacgt attcccaatc cgccaccaag atcgaggtgc tcgatatgca gtccccgagc
      721 ctcaaggcga tcgagcaatt cgagcatttt tccggatgct tcaatgaagg ggtcaagctc
      781 ggtaagctgg gagccaatgt ggcaatccag ccctgtcatg cggatattcg gaaattgccg
      841 atacgaggca tacaaggatt ctgcctgatc gactggaatc ccaaacttgt tttccttgag
      901 gcccgtggaa atatatcgat gcgtttttgc atccacatct ggattgacgc gcaggctgac
      961 cggagcgatt ttattcattt cacctgcaac ccgatcaagc acctctaatt ccgcttccga
     1021 ttccacatta aagcagaata ttcccgcctc aagcgccgct cgcatttcat gcgcctgttt
     1081 gcctaccccc gaaaaaacga tttttcgcgg atcgccaccg gctttcagta cgcgctgaag
     1141 ttcaccgccc gaaacaatat cgaagccgct gcccaggcgg gcaaacagat tgagaatcga
     1201 caggttggag ttggctttga ccgcgtagca tatcaggtgg tcacgcgctg caaacgctgc
     1261 gtcgaattcc tcgtacgcgg caataagggt ggaccgggaa taaacataac agggagttcc
     1321 gaccgcagct gcaatgcgat ccagcggaac cgattcaccc caaagcctgt tatcgcgata
     1381 agcgaacgct gaagcgctca cgtctccgct cacttcttct gatcctgggg cgcttgtttc
     1441 gactggacct caccttgctg ttgcggaagg taaagcgggg ccttgagacc gcaggcacca
     1501 agcagaaggg cgatcagaac aacggtgcag taattacgca tatcaggttt ttagcaggaa
     1561 atggcgaata gcagaggata ctagcataaa gaccagaatg ctttgatatc cctccctatc
     1621 cttgcagttg cccatagctt tgtccatagc tcttcaattc tcttctggca gtgacgtagt
     1681 cgggatagat gcgttccacg atccgccaga aatccgaaga gtggttcatt tcaatgaggt
     1741 gggacaactc gtgcgcgacc acgtagtcga tcaaatggag cggcttctgg atgagccgcc
     1801 aattcaggcg cacgacgcct ttggcggtgc agcttcccca caaagtgcgc gcggacgaca
     1861 gcttcagttc tcgcacagaa acgccgagct tgggtgcata gtatgcgact cgctcggtga
     1921 aacatgccaa cgcttggctt cggtaccagt tcatgacgac gtcttcgatt tgacgggggg
     1981 taagcaggga gggcagtcca agcccggctt gcctgcattc gacccgggtg ttttccgata
     2041 accgcaccat gtgggctgat cccttgactg cagcaatcag cttccatggt tctccaagca
     2101 gtggaaaaat cgctccttct tcccaaacgg gaccgcgcgg tttcctgtct ttccactcct
     2161 ccagcttttt aacgatccag tcggctctct tttgcagtac cgattcgacc cagcccatcg
     2221 actctctcat cggaatacga accgtcaagc cgctgctgtt gattctcatg ccaatggttt
     2281 tccgcctgca ccgcatgagt acatacggca cctcgttgcc gttgaggttg attcgccgga
     2341 gctcactcgc tgataatcca gccgtcctta aaattgcgcg tgaattgtct tccatatgcc
     2401 atgccacgtg atttacggtt tccgatgccc tatgcgctcc acctctgcct cgatccatga
     2461 ttccactcgg gcattgagct cgtccggttc cattcctgtt ggatcgattg gagcgccaat
     2521 gcttactgta atggtgcccg gaagtttgat cagggcattt tttccccaat actccccggc
     2581 gttatgcgcc acagggacga cggccacatt cgtatgagtc gcgagccatg cgccaccgat
     2641 gcggtatttg cccttgctac ccggcgcaat ccgggttcct tctggaaaaa taacaatcca
     2701 gaaacccttc ttcagccgat cctttccctg ctcgacaatt tgcttgagcg ccgctttacc
     2761 cgagcttcgg tcgattgcga taggactggt catggccaag ccccagccga agaatggcac
     2821 gagcagcagt tcttttttta gcacccatac ctggggcggg aagatttgct gaaacgcaag
     2881 ggtttcccat gcggattgat gcttcgacag tatgatgctc ggcgtctgag gaatatgttg
     2941 gcctccgatt acccggtaat cgattccgca cacgacccgc agaagataca gcatgaagtg
     3001 cgcccacccc gaagtgatgc ggtagcgctt cagcggcggc agtggaaacg cggccagcac
     3061 aatcagaaaa taaaacggtg tgatggcgat ctgcaacaac acgtaaatcg ccgaccgcag
     3121 cactgtaaga accatgccta ctctatcaac gcatcaaccg ctgccgccag gttgggaaaa
     3181 acccgggtgt tttcgggcaa ccccccttcc tgcctggttt tttcgccctt gcctgtcaag
     3241 acaaggatcg gaatggcacc gaccgctgcg gccgcccgca ggtcgcgcaa cgaatcgccg
     3301 atggcgggca cgtttctcag cgtcagcccg aagcgttcgg aaatttcctt gaacattccg
     3361 attgccggct tgcggcaatt gcacttgtcc gcattggcgt gagggcaata aaacatggcg
     3421 tcaatgcggc cccctacctg gttaaccgct ttgcacatct tgtcgttgat tgcattgagg
     3481 gccaccatat ccaacaatcc ccggctaatc cccgattggt tggttgcaac aaccacgcgg
     3541 taccccgact gggtcagatg ggcgatcgcc tccaggctgc ccggaatggg tttccattca
     3601 tccggcgtct tgataaatgc cgcgctgtcg taattgatga cgccgtcgcg gtcgagaata
     3661 gcgagtttca tagccgtatc aaaccgccag cctggaaata tcggcaaccc ggttcattgc
     3721 cacgcgcagg gtggcgagca acgccagccg gttatttcgc aaggtctcat cctcggtgtt
     3781 gaccataacg tgatcgaaaa actcgtccac cggttgttta agcgcagcca gaatctgcaa
     3841 tgagctgctg tagtcgccgg cctcaaaatc cgcgtcagcc tcgggaacaa tgcgggccaa
     3901 tgctgcgtac aacgcacgct ccgccggtgc ctgcaggagc ttgctatcca ccctcttttc
     3961 tccgctcaca cccgcttttt tcaggatgtt gccaacacgc ttgttcgttg cagcaagact
     4021 ttccgcttcc ggcaatgcgg caaaggcacg caccgcatcg aggcgttttt tgacgtcatg
     4081 cagccgcctc ggtcgcaaag ccaataccgc atcgatctgc tgaggagagt aaagctgaac
     4141 gcgcgagttg gcgatatcac tgccttggct gcagcccgaa gagctttgat cctggtaagc
     4201 ttcgccaaac aagtgcctta tgcgctcata gatgaaatcc gtcaagactg gagtcggatt
     4261 attgatcctg tttccaaaaa tactgaggac ctgctcaagc agagcatcca aggccagcgg
     4321 gagttctctt tcgatcaata tgcggatcaa tccgagggca tgccggcgca gtgcgaacgg
     4381 gtctttgtcc ccggtgggta tttgtccgat accgaacatg cccactaggc tctccagcct
     4441 gtcggccagt gcgatgcaga cgcccaccct gttgcggggc aaaacgtccc ccgcgaaacg
     4501 gggcttgtaa tgatcttcaa ttgcgtccgc cacatcctcg gcgacgccgt cgtggaggga
     4561 ataatagcgc cccatgatgc cctgcagctc gggaaattcc gcaaccatgc cggtgagcaa
     4621 atcggctttg cacagcatcg cagcttcccc cgccttggct gcaagttcat tcccgtccaa
     4681 ttcgaggccg atcgcctggg cgattgccca gatacgctcc acgcgctctg ccagggttcc
     4741 cagcttatgg tgatacacca cctctcgcag cccttccacg cgtgacgcca gggttttctt
     4801 ccggtcctga tcgaaaaaga attgggcgtc cgccagccgc gagcgcaaca cgcgctcgtt
     4861 gccggagatg atctggctgg catcagctgg atgaatatta ctcacgatga gaaacttgtt
     4921 tgaaagcttc ccgccgacat ccagcaaagg gaagtatttc tgatttgcct tcatcgtcag
     4981 aatcaggcac tcattgggca cttccagaaa ttcccgctca aattcccctg ccagcacatt
     5041 agggcactca accaatcccg tgacttcatc cagcagcgcg tcatcttctg cttttttcaa
     5101 gcctagtctc gcagcagcat catccagctg atggactatc tccccccgcc ggtccttgaa
     5161 gctcgcaata act
//
LOCUS       DASSHJ010000256        10784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1249931,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000256 DASSHJ010000000
VERSION     DASSHJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249931"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..696
                     /locus_tag="VFI43_09900"
     CDS             349..696
                     /locus_tag="VFI43_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7062478.1"
                     /translation="MKIKATVEEVLARIPGPATPEWPIGERFAIGLAHGSMSVEYYAP
                     VGNDPQTPHEQDEVYFIHKGRGELVIEGDRYPFNAGDCLFVGAKVEHRFENFSEDFGT
                     WVVFWGPKGGEKE"
     gene            970..1230
                     /locus_tag="VFI43_09905"
     CDS             970..1230
                     /locus_tag="VFI43_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HET7062479.1"
                     /translation="MNHCLQAGGKHVEAHHFRLMMNCAEICQTSTNFMLSGSDFSKQL
                     CKVCAEVCDACARSCEQVGDMEDCVKACRECAQHCREMTGSA"
     gene            complement(1461..2408)
                     /locus_tag="VFI43_09910"
     CDS             complement(1461..2408)
                     /locus_tag="VFI43_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7062480.1"
                     /translation="MNNNGAGDSPAEDRSEGPSHAHRNHQHHPHGHGRAFVIAICLNV
                     TFVVVEFAYGFIANSTALMADAGHNLSDVLGLALAAGASILASKAASENYTYGLRSSS
                     ILAALGNAVLLLVACGAIAWEAIHRFLQPPAVAGLTVTLVALIGVVINGFSAWLFIKG
                     SKNDLNIRGAYLHMAADAAVSGGVVLAGIAMMLTDQYWLDPAVSLLVVAIVIAATWGL
                     LRDSLRLALNAVPGHIEISRIDAYLRERPGVVDIHDLHIWGLSTTESALTVHLVMPNG
                     YPGDAFMENIRETLNVRFSIHHCTIQIEQGTVNHACSLE"
     gene            2679..3056
                     /locus_tag="VFI43_09915"
     CDS             2679..3056
                     /locus_tag="VFI43_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062481.1"
                     /translation="MRKRRHIYSVMFALLVSMLTNAFGWAFNGEVFAHELDHEHHVLS
                     LDPATHLEAHQRAVRNDEGHLDAATHLCLHAAGQYQPFFFTAPPRVPPSLGMEVLHVF
                     VSVAIPESIPDSPLHPPRSIFQS"
     gene            3217..4584
                     /locus_tag="VFI43_09920"
     CDS             3217..4584
                     /locus_tag="VFI43_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7062482.1"
                     /translation="MNLCASFTDKWRFARVGLIILGLCCPFAALSQNTFSGGVAGDAM
                     VTSPADEKNTLTLRDAIKLALQRNPDLAAFDKEVLALGGATLQAGLFRNPELDVVTED
                     VGSPVRPEPVVQQFVTMRLSQLIELGGKRAARVNAASLGEELARKDYEARRLDLITMV
                     ANFFTDVLTGQERVRLAEDSIHLAQKVVDSTSKRVQTGKAPPLEETKAKMALSTTGIE
                     LELARRDLIAARKQLSLLWANPLPKFDKVTGYLESSILLPSYEELSRRVLNSPMALRS
                     AKNVEQRKALLEVENTRQVPDLIVNAAVRRYLQTDNVTALAGFTIPLPLFNRNQGSIR
                     EAHQRLDKAIDEQMATDLRLNALLSKTYEALLGAQNEIKVLREDVLPGAKSAFNVASI
                     GYELGKFSFLEMLDAQRTLFQNQLLYVRSLAAHQKLLNEIERLIGGAIDGQSPVDRAD
                     QNLQE"
     gene            4585..6117
                     /locus_tag="VFI43_09925"
     CDS             4585..6117
                     /locus_tag="VFI43_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7062483.1"
                     /translation="MVKKKQQIAIVAVVAIGIVLGTVILTADRRTSPSGAEASGEAAH
                     GHETTGKNASAYPVEETAIGPHGGRLLAADGLGVEITLYETGVPPQFRIYLHERGKPL
                     PPTAAKVTVTLLRLGQPAQVYTFKPEADYLVGDHPVEEPHSFDVAVAAEWKGKRLHWK
                     YAQAEMRVEIPDDMLKSMGIEILTAKPETIRTALKLPGEIVFNEHTIVQVVPRLPGIV
                     TAVYRHHGQHVEKGDALAVIDSQMLAELRSQYLVAQKRLGLARTTFEREKRLWEEKIT
                     AKQDYLTAQELLTEAEIAVELAAVKLRSLGVRPETNGRGENLARFQILAPISGLITEK
                     AIAQGQTLKEDDNIFTIADVSTVWAAVTVYPKDLAVIRVGQRVTIKATAFDISGEGPI
                     TYISTLIGGQTRTATARVELDNKDGRWRPGMFITAELVTGEIPVAVAVETSAVQTLGD
                     WTVVFGRYGPYFEARPVELGRSDGKLVEVLRGLHARENYAAGNSFAIKAELGKAGASH
                     QH"
     gene            6146..9355
                     /locus_tag="VFI43_09930"
     CDS             6146..9355
                     /locus_tag="VFI43_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET7062484.1"
                     /translation="MIERVLQISIQRRGLVLLAVLAMALVGIYSYQKLPIDAVPDITN
                     VQVQINTTATGYSPLEVEQRVTFPMEIIMAGLPNLEYTRSLSRYGLSQVTVIFRDGTD
                     IYLARQLVSERIQRARGQLPLGIEPSMGPISTGLGEIFMWTIRAEEGAKKADGTKYTL
                     TDLREFQDWIIRPHVRTVPGVAEVNSIGGFVKQFHVAPYPERLISYGLTLQDVIAALS
                     RNNINVGAGYIEKSGEQYLVRVPGQVANLDEIGNITLGSRGGIPIRIKDVADILIGKE
                     LRTGAATLNGKETVLGTAHMLIGENSRVVSQAVAKKLAEVNGILPEGIAAVVAYDRTV
                     LIDKTIRTVATNLLEGAALVIAVLFLFLGNLRAAIITSLVIPLSMLFTFSGMLVDGVS
                     ANLMSLGALDFGLIVDGAIVIVENSIRRLAHEQIRLGRELTPLERFTTVFDATREARR
                     VLIFGELIIMVVYLPIFALAGVEGKMFHPMAFTVVTALLGAMILSVTFVPAAVALFLS
                     GKVAEKESAAVAWAKRTYTPLLTGVMSNRSLTVTTAIVVVILSGLLTTRMGSEFIPSL
                     NEGDIALHAIRIPGTSLTTAVEMQDELEATILKFPEVERVFSKLGTAEIATDPMPPSV
                     ADTFIILKPRTEWREAYSTKTELIEAMEQAVKKVPGNNYEFTQPIQMRFNELLSGVRA
                     DVAVKVFGDDMDTLFILAEQIEELLSTVPGAADVKIEQLMGLPVLSIQPDRDKLARYG
                     LNVGDVQDTVTVAVGGQTAGMIFEGDRRFELQVRLPEQLRGDIESLRRLPLGLPLPAA
                     APGAAPPVPAVPLAAAPAYVTLEEVAVLKVVQGPNQISRENSKRRAVVTANVRGRDLG
                     SFVEEAEALVRSKVQVPAGYWLTWGGQFEQLVSAAKRLQIVVPLALSLILVLLYAMFG
                     NFRDGLLMFTGVPFALSGGIVALWLRGIPLSISAGVGFIALSGVAVLNGLVMLAFIRE
                     LRTGGMSLDEAIRHGALARLRPVLMTALVAALGFVPMALATGTGAEVQRPLATVVIGG
                     IISSTALTLLVLPVLYRLVHGRDKGAGIPQKALVPELAN"
     gene            9552..10769
                     /locus_tag="VFI43_09935"
     CDS             9552..10769
                     /locus_tag="VFI43_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HET7062485.1"
                     /translation="MKKPRASHRTRSPRIVQELVRLAVGLAASGSRIEDVFWENQLAV
                     KIRAQLRTGDDQNLEAALDQLYDSDTVAYGEFIYSIEAAIECATVTRGDRDYDLLMFA
                     IPLLTWSRFSIPSGPMSENVLTNLRAKMQQLVLMRDTQFALADCLFSPDQLPRGYHLT
                     HELAGKLWSAALAGESVLHMNPRHLAETGRFLSDTRYVLGAVAVQHGKPMFRWQAEGS
                     GDGKRSDILNGSKSGKDEILEPWQREAAEAMRPLFQGCAFEVLNPEGFFSAWRMADRL
                     ARPYAMHATVDFLQTSLNISASRLRAIVAPFYDQWLEEYRISFTLKDRDNVLYGLVWA
                     LVGDEDENSDIVRQIETTLRECGITEMTALEQRFILEYCEDCGAPLFPNPEGEVVHAE
                     FPEEGEATPIHLH"
BASE COUNT         2473 a         2967 c         2957 g         2387 t
ORIGIN      
        1 accccacgtc aaccagccgg aacttcagct ctgcggtatt cgagtgctga taacccctat
       61 cccttgcacc acgggcaaga ccaagaatgc gtcccacagt ggagacctca tcgaccctat
      121 cctttgcgcg catgggcgcg accttctaca attagttctg actacaatca gttctgactg
      181 aactgaacaa tgttgataga taatcagagc cagcccagca cactcttttc ccaggaccat
      241 gctgatcgcg aattgcgctt ctccacctat tgctgagtcc gggtattcag ggtgagcccg
      301 tctataagag tgactccgct gttcaatttt taactgagga gtttcgattt gaaaatcaag
      361 gcaaccgtag aagaagtcct tgcgcgcatc cctggccccg ctacgccgga gtggcccatc
      421 ggcgaacgtt ttgccatcgg ccttgcccat ggttcgatgt cagtcgagta ttacgcgccg
      481 gttggtaacg atccccagac gccccatgag caggatgaag tctatttcat ccacaagggc
      541 aggggcgagc ttgtcatcga gggggatcgg taccccttca atgcgggtga ttgtctcttt
      601 gtaggggcca aggtcgagca tcgtttcgaa aacttttccg aagactttgg aacatgggtc
      661 gtgttctggg gcccgaaagg cggggagaaa gaataaagct gcaaatagtc gccgaacaat
      721 tcgcttgctg catggtttgg cggagggaag cggcttcagg tgctcttaat aaaaatcaaa
      781 agctattgct gcttagcgta cagacttttt agagcagcag cttcagcatt tgactggctt
      841 tttcttgaac cccctatttg ttaaatatat tctcaaggag acctcgctat gactcacact
      901 accgatccta aaatgcactc gtgtgttgat gcgtgcaacc ggtgccacca aacatgtctg
      961 cacgaagcga tgaatcattg ccttcaggca ggcggcaaac atgtcgaggc ccatcatttc
     1021 cggttgatga tgaattgtgc tgaaatctgc cagacctcga ccaatttcat gttgagcggt
     1081 tcggatttta gcaaacagct atgcaaggtc tgcgccgagg tctgcgacgc gtgcgccagg
     1141 agttgcgagc aggtgggcga tatggaagat tgcgtcaagg cgtgccgcga gtgtgctcag
     1201 cattgccgtg aaatgactgg ctcggcttag ccgggcgtcg ctgctcagga gcgttcaaga
     1261 cgatttatag cgggtttgcg aggagcggta tgtctcacca aattatccaa ccagccatcg
     1321 cttgccttgt cgttcattcc ccgtaacacg cgatgctgtt tttacaaagc ccggtttttc
     1381 aatttccttc gtaccgtctt attcagcggg agtatcccga attgcggccg ccgggcttct
     1441 tctgtcagcc acaagttatt ctactcaagg gagcaggcgt ggttcaccgt tccttgttcg
     1501 atctgtatcg tgcaatgatg aattgaaaag cgaacgttca gggtttccct gatattctcc
     1561 atgaaggcat cccccggata tccgtttggc atcaccagat ggaccgtcaa agcgctttcc
     1621 gtcgtgctca gtccccagat gtgcagatca tggatatcca ctacacccgg tctctcgcgc
     1681 agatacgcgt caattctgga tatctctata tgccccggca cggcattcag agcaagccgg
     1741 agcgaatcgc gaagcagtcc ccaagtagca gcaatcacta tagcaacgac gagcaggctg
     1801 acagcaggat cgagccagta ctgatccgtc agcatcattg ctatacctgc aagtaccact
     1861 ccccccgata cagcagcgtc cgccgccatg tgcaaatagg cgccccgaat attcaaatca
     1921 tttttactgc cctttataaa caaccatgcg gaaaagccgt tgatgacgac tccgataagc
     1981 gccaccagcg taacagtcaa gcccgcaacg gcgggcggtt gcaaaaagcg gtgaattgct
     2041 tcccaggcga ttgccccgca agcgacaagc agcagcacgg catttcccaa tgcagccaga
     2101 atcgagctac tgcgtaggcc atatgtatag ttttcgctcg cagccttgct cgccagaatc
     2161 gatgctccag ctgcaagcgc gagacccagc acatcggata agttgtgtcc cgcatccgcc
     2221 atcaacgctg tggaattggc aatgaaacca taagcaaact cgaccacaac aaaggtcacg
     2281 tttagacaga tggcaataac aaaggcgcgg ccatgaccat gcggatgatg ctgatgattg
     2341 cgatgggcat gagacgggcc ttctgaacga tcttcggccg ggctatcacc tgcaccatta
     2401 ttattcattc ggaaaagatt ctgggttcgt aaagcagtcg tactaagcat agcgcgcata
     2461 attgagacaa cgccgcgtta ttcggccttg caagcgcgct cgtcatccag gcgaagacgt
     2521 cctccacctg gcaatctggc aggatcattt tgtcgaacat tggtccgatc ctccgaatgc
     2581 tcaaatatct catcaattcc acatcggtct ttttcagaat gcagccctga aaacaatatt
     2641 ggcattaccc ggctcagggg tataaaatcg cgtaccttat gagaaagcgc cgccacatct
     2701 attcggtgat gtttgcgcta ttggtgagca tgctcaccaa tgccttcggc tgggcgttca
     2761 acggcgaagt attcgctcat gagctcgatc acgagcatca tgttctctcc ctggatcctg
     2821 caacccatct cgaggctcat cagcgggccg tcagaaacga tgaaggccat ctggacgcag
     2881 cgactcatct gtgcctgcat gcggccggac aatatcaacc gtttttcttt accgctcccc
     2941 cgcgcgtacc cccatcgttg ggcatggagg tattgcacgt gtttgtgtcg gttgccattc
     3001 ccgagtcgat ccctgactcg cctcttcacc ctccccgcag tattttccaa agctaagcaa
     3061 taaacaacgg ctgttgcaag tggctggcgt ccatgcgtta tctgcacacc gccaactgtc
     3121 tgacattgca ggtcggtctt gatgagcatc ttttgcccat cgcagacgtg tcgcatgcga
     3181 ccgatatcga ttcatataat gctgcggaga atcctgatga acctttgtgc ttcgttcacg
     3241 gataaatggc gcttcgcccg cgtagggcta attatccttg gactttgctg cccgttcgcg
     3301 gcgctttcgc aaaatacctt ttccggcggc gtagctgggg acgcaatggt cacgtctccg
     3361 gcggacgaaa agaacaccct gactctacgt gatgccatca aactggcatt gcagcgcaac
     3421 ccggatctgg ccgccttcga taaagaggtt ctcgcactgg gaggcgcaac gctacaggcc
     3481 ggtttatttc gcaaccccga actggacgtg gtgacagaag atgttggctc tccggtgaga
     3541 ccagagccgg tagttcaaca atttgtaacg atgaggctta gccagcttat cgaactcggg
     3601 ggcaagcgcg cggctcgggt caacgcagcg tccctcggcg aggagctcgc gagaaaggat
     3661 tacgaggccc gccgcctgga cctcatcacg atggtggcaa atttttttac tgacgtattg
     3721 acgggacagg agcgagtaag gctagccgaa gatagcatcc atctagccca aaaagtcgtt
     3781 gattcaacca gtaagcgagt ccagaccggc aaggcgcctc cccttgagga aaccaaggca
     3841 aaaatggcgc tctccaccac cgggatagaa ctggagctgg ccaggcggga cctgatcgcc
     3901 gcacgcaagc aactgtccct gctatgggcg aatccactgc ccaagttcga taaggtaaca
     3961 ggatatctgg agtcgagtat cttgttgccc tcttatgaag aactttccag gcgcgttctc
     4021 aacagcccta tggccctgcg gagcgcgaaa aatgtagaac agcgcaaggc gttgctggaa
     4081 gttgaaaaca cgcgtcaggt tcctgacctc atcgttaatg cagccgtcag gcgctacttg
     4141 caaacggata acgtcactgc cctggcgggc tttaccatac ccctcccact attcaaccgc
     4201 aaccagggca gtatcaggga agcgcatcag cgcctcgata aggccattga tgagcaaatg
     4261 gccacagatt tgcgtctcaa tgccctgttg tccaaaacct atgaggcgct gctgggcgca
     4321 caaaacgaaa tcaaggtact gcgggaagat gtgctgcctg gagcaaagag cgccttcaat
     4381 gtcgcatcca ttggatatga gctgggcaaa ttcagcttcc tggaaatgct cgacgcccag
     4441 cgcaccctgt ttcagaacca gctcctatat gtacgctcgc tggctgccca tcagaaactg
     4501 cttaacgaaa tagaacgtct gattggcggc gcaatcgacg ggcaaagccc tgtagaccgc
     4561 gctgatcaaa atcttcagga gtagatggtg aagaaaaaac aacaaatcgc catcgtcgcc
     4621 gtggtcgcga taggtatcgt gctgggaacc gtaatcctga cagcagatcg ccgcacatcc
     4681 ccttcgggag cagaggcttc tggggaggcg gcccacgggc acgaaaccac aggtaaaaat
     4741 gcaagtgctt atcccgtgga agaaactgcg ataggccctc acggcggccg tctcctggca
     4801 gccgacggcc ttggcgtgga aatcacgctc tatgaaacag gcgtgcctcc acagttcagg
     4861 atttatctgc acgaaagggg aaagccattg ccgcctactg ctgccaaggt cacggttacc
     4921 ctgctgcggc tcggtcaacc ggcgcaggta tatacgttca agccggaagc tgattacctt
     4981 gtcggggatc atccggtgga agagccgcac tctttcgacg tagctgtcgc tgctgaatgg
     5041 aagggaaaaa ggctgcactg gaaatatgcg caggctgaaa tgagggttga aattcccgac
     5101 gatatgctga agagcatggg aatcgagatt ttaaccgcca aaccggaaac gatcaggacc
     5161 gcgctcaagc tgccgggcga gatcgtcttt aacgaacata ccatcgtaca agtcgtccct
     5221 cgccttcccg gtattgtcac tgccgtctat cgtcatcatg gccagcacgt ggaaaagggt
     5281 gacgcgcttg cagtcattga cagccagatg ctggccgaat tacgcagtca gtacctcgtt
     5341 gcgcagaaaa ggttaggttt ggcgcgaaca acatttgaac gcgaaaaacg gctgtgggaa
     5401 gaaaaaatca cggccaaaca ggattatctg accgcgcagg aattactgac tgaagctgaa
     5461 atcgcagttg agctggcggc ggtaaaactt cggtctcttg gcgtgcggcc tgaaacaaat
     5521 ggccgaggag aaaaccttgc ccgtttccag atccttgctc ccatctccgg gctgattact
     5581 gaaaaggcga tcgctcaggg ccagacgcta aaggaggatg acaatatctt tacgatcgcg
     5641 gatgtctcca cggtgtgggc cgctgttact gtatatccga aggatctggc ggtgatcagg
     5701 gtggggcaaa gagtcactat caaggcaacc gcgttcgata tttccgggga aggccctatc
     5761 acctacatct ccactctcat tggcggccag actcgtactg caacggccag ggtagaactg
     5821 gataacaagg acggaagatg gcggcctggc atgtttataa ccgctgagct tgtcactggc
     5881 gagattccgg ttgccgtcgc tgtggaaaca agcgccgtcc agaccctggg cgactggacg
     5941 gtagtgttcg ggcgttatgg accgtacttt gaagcacgcc ccgttgagct cgggcgcagc
     6001 gatgggaaat tggtcgaagt ccttcgcggg cttcatgctc gcgagaacta tgcggccggc
     6061 aacagttttg cgatcaaggc ggaactgggc aaagcaggcg caagccacca gcattgacac
     6121 cagcattgat ggaggcgcta aaaaaatgat tgaacgagtg ctgcaaatat ccatacagcg
     6181 ccgcggtctt gtcctcctgg ccgtgctggc tatggcgctc gtggggatct acagctatca
     6241 gaagctgccg atcgacgcag tgcccgatat tacgaacgtg caggtgcaga tcaataccac
     6301 cgcaactggc tactcgcctc tcgaagtcga gcaacgggtg accttcccca tggaaatcat
     6361 catggcaggt ctccccaatc ttgaatatac ccggtctctc tcccgttacg ggctgtcaca
     6421 ggtcacggtt attttcaggg acggcacgga catctatctc gcacggcagc tcgtaagtga
     6481 gcgaatacag cgagcccggg gccagcttcc cctgggtatc gagccctcga tgggccctat
     6541 ttcgaccggc ctcggcgaaa tattcatgtg gacgattcgt gcggaggaag gagcgaaaaa
     6601 ggcggatggc acaaaatata cgctcacaga cctgcgtgaa ttccaggact ggatcatccg
     6661 cccccacgta cgtacagttc ccggcgtcgc tgaagtcaac tccataggcg gttttgtcaa
     6721 acaattccat gttgccccct accccgagcg gctgatctca tatggactga cgctgcagga
     6781 cgtcattgct gccctgagtc gcaacaatat taacgtcggt gccggttata tcgaaaaaag
     6841 cggcgaacag tatcttgtgc gagtaccagg gcaggtcgca aacctggacg aaatcggaaa
     6901 tatcaccctc ggttcgcgcg ggggaatacc gatacgcatc aaggacgtgg cggatatcct
     6961 gatcgggaag gaactgagaa ccggtgctgc aaccctcaac ggcaaggaga ccgttcttgg
     7021 cacggcgcat atgctcatcg gcgaaaacag ccgggttgta tcgcaagctg tcgcgaaaaa
     7081 actggctgaa gttaacggaa tccttcccga aggcatagcc gcggttgttg cgtacgaccg
     7141 tactgtcttg atcgataaaa ccattcgaac tgtcgccacc aacttgcttg agggtgcagc
     7201 gctcgtcatc gccgtgctgt tcctgtttct gggcaatctg cgcgccgcaa tcatcacttc
     7261 gctggttatt cccctgtcaa tgctgttcac cttcagcgga atgctggtcg atggcgtcag
     7321 tgcgaatctc atgagtctgg gagcactcga tttcggcctt attgtcgatg gcgccattgt
     7381 cattgtcgaa aacagtattc gtcggctggc gcatgaacag attcggctcg ggcgtgagct
     7441 gacccccctg gaacgcttca ccaccgtttt cgatgccacc cgggaagcaa ggcgtgtgct
     7501 catctttggg gagctgatca tcatggtggt ctacctgccc atcttcgccc tcgccggcgt
     7561 ggaaggcaaa atgtttcatc ccatggcatt taccgtagtc acagcgctac tgggagcgat
     7621 gattctctcg gtgacgtttg ttccagcggc agtcgcactt tttctctcgg ggaaagtggc
     7681 ggaaaaggag agcgccgcgg ttgcatgggc aaaaaggact tatacgccgt tgttaaccgg
     7741 ggtaatgagc aatcggagcc tgacggtaac gaccgccatc gtagttgtga tcctatccgg
     7801 tctgctgaca acgcgaatgg gcagcgaatt catacccagc ctcaatgagg gagacatcgc
     7861 gcttcacgcc attcgcatac cgggcacaag cctgaccacc gctgttgaaa tgcaggatga
     7921 attagaggcg acgatcctga aatttcccga agtggaacgc gttttttcga aattgggaac
     7981 cgccgaaatc gcgacggacc caatgcctcc gagcgtggca gatactttca tcattcttaa
     8041 accgcgaacc gaatggcggg aagcctactc cacgaagact gaactcatcg aagcaatgga
     8101 gcaggcggtc aaaaaagtgc cggggaacaa ctatgagttc acgcaaccga tccagatgcg
     8161 atttaacgaa ctgctatcag gggtacgcgc ggatgtggcg gtaaaagtct ttggcgatga
     8221 catggacact ctgttcatcc ttgccgagca gatcgaagaa ctgctctcta ccgtccccgg
     8281 agctgcggat gtaaagatcg aacagctcat gggactgccg gtgctttcga ttcagccgga
     8341 tcgggacaaa ttggcacgct acgggttgaa cgtaggtgac gtacaggata ctgtcacggt
     8401 ggccgtaggt ggtcaaaccg cggggatgat tttcgagggg gaccgccgct tcgaactcca
     8461 ggtgcggctt ccggaacagc ttcgcggcga cattgaatct ctcaggcgct tgcccctcgg
     8521 cctgcccctc ccggcagcgg caccgggagc agccccgccc gtccccgcag tgccgctcgc
     8581 agctgcgccc gcctacgtca ccctcgagga ggtggccgtg ctgaaagtgg tgcaaggtcc
     8641 caaccaaata agccgggaaa acagtaaacg cagggccgtc gtcaccgcga atgtccgtgg
     8701 acgcgatctc ggctcctttg tggaggaagc tgaagcactc gttcgatcca aggtgcaggt
     8761 tccagcggga tattggctga catggggagg tcagttcgag caactcgttt ccgccgcaaa
     8821 gcgccttcaa atcgtcgttc ctctggctct cagcctgata ctggtactgc tttacgcgat
     8881 gttcggcaat tttcgggatg ggctgttgat gtttacagga gtccctttcg ccctcagcgg
     8941 cggcatcgtt gcattgtggt tgcgcgggat acccctttcg atttccgcgg gagtcgggtt
     9001 tatcgctctg tccggtgtag cagtcctcaa cggcctcgtc atgctcgcgt ttatccgcga
     9061 actgcgaacc ggcggcatga gcctggacga agcgatccgg catggcgcgc tggcgcgctt
     9121 gcgtcccgta ctgatgactg ccctggtagc ggcgctcgga ttcgtcccca tggcgctcgc
     9181 gaccggcacg ggggccgaag tacagcgacc cctcgcaact gtcgttatcg gggggatcat
     9241 ttcatccacg gcgttgactc tgctggtatt gccggtgctg taccgcctcg tgcatggaag
     9301 ggataagggt gctggaattc cgcaaaaggc gttagtgccc gaactggcaa actgagaagg
     9361 gagttttcga ttacggttcc gcgcaccaac ggcatgagca aacaatcccc gctgcttctc
     9421 ctcggtgcat ccttcagggt acttgtattt cttcaagccg cttgtgctcc cggatatcaa
     9481 gcctgttgac caggttgagc atttcgttgc gaaatttagg ttaaaatgtt tttttccagc
     9541 catttatctc catgaaaaaa ccccgtgcat ctcatcggac aaggtcgcct cgcatcgtcc
     9601 aggagcttgt ccggcttgcg gtgggacttg cagcttccgg cagccgcata gaagacgtgt
     9661 tctgggaaaa tcagcttgcc gtaaagatac gggctcaact gcgcacgggc gatgatcaaa
     9721 atctcgaagc tgcattggac cagctttatg acagcgatac ggtggcgtac ggggaattta
     9781 tctattcgat tgaagcagca atcgagtgcg ctacggtaac ccggggcgat cgtgattatg
     9841 atctgctgat gtttgcgatc cctctgctga cgtggtcacg attctcgatc ccctccggac
     9901 cgatgtccga aaatgtgctc accaaccttc gcgccaagat gcaacagctg gtgctcatgc
     9961 gagacaccca gtttgcattg gccgactgcc tcttcagtcc ggaccaactc ccccgcggct
    10021 atcatctgac acatgagctg gcgggcaagc tgtggtccgc agcgctggcc ggggaatccg
    10081 tattgcatat gaatccgcgc catctggctg aaaccgggcg attcctgtcg gacacgcgtt
    10141 acgtacttgg agccgtcgcg gtccagcatg gcaaaccgat gttccgctgg caagccgagg
    10201 gatcgggcga tggcaagcgg tccgatatac tgaatggatc gaaatcagga aaagacgaga
    10261 ttctcgagcc ttggcaacgc gaggccgcgg aagcgatgcg tcccctattc caggggtgcg
    10321 cgtttgaggt actgaatccc gagggctttt tttctgcctg gcgcatggcg gatcgtctcg
    10381 cccgaccgta tgcgatgcat gcaaccgtgg attttctgca aaccagcctc aacatttctg
    10441 cgtcgagatt acgcgcgatc gttgctccct tttacgatca atggctggag gagtaccgta
    10501 taagcttcac gctcaaggat cgcgataacg tgctttacgg actcgtctgg gcgctcgttg
    10561 gcgacgagga tgagaattct gatattgtcc ggcaaatcga aacgaccttg cgtgaatgcg
    10621 ggattaccga aatgacggca ctcgaacaac gatttatcct cgaatattgc gaagattgcg
    10681 gcgccccgct attcccgaac cccgaaggtg aggtcgtgca cgcggaattt ccggaagaag
    10741 gcgaagcaac tccaattcac cttcattgag ctacatatgc atcc
//
LOCUS       DASSHJ010000257         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_926713, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000257 DASSHJ010000000
VERSION     DASSHJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926713"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..904)
                     /gene="cysM"
                     /locus_tag="VFI43_09940"
     CDS             complement(17..904)
                     /gene="cysM"
                     /locus_tag="VFI43_09940"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HET7062486.1"
                     /translation="MFKTIEDFVGNTPLVKLKHLPGITSNTILVKLEGNNPAGSVKDR
                     PALSMITRAQERGDIKPGDTLIEATSGNTGIALAMAAAIMGYPLILIMPEHLSIERRQ
                     SMRAYGAEIILTSKEGSMEEARDVAERMRDEGRGVILDQFASPDNPRAHYEGTGPEIW
                     RDTEGTITHFVSSMGTTGTIMGCSRYFKEKKPEIQIIGVQPEEGAQIPGIRKWPEAYL
                     PKIYDSSRVDCLMRVSQAEAEDMTRRLAREEGIFAGISSGGALAAALKISAEVHNAVI
                     VSIVCDRGDRYLSTGVFPA"
     gene            complement(1098..1388)
                     /locus_tag="VFI43_09945"
     CDS             complement(1098..1388)
                     /locus_tag="VFI43_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7062487.1"
                     /translation="MKKLLFIILLAFFGSALAGVNINTATQAELETLQGIGPAKAKAV
                     IEYRKKNGPFKSPDDLEKVNGIGPAIMKRVRKDITVGSGAAAARKENKLPVR"
     gene            complement(1469..1927)
                     /gene="ssb"
                     /locus_tag="VFI43_09950"
     CDS             complement(1469..1927)
                     /gene="ssb"
                     /locus_tag="VFI43_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7062488.1"
                     /translation="MASVNKVILIGNLGKDPETRYMPNGEAVTNITVATTETWKDKNG
                     EKQEKTEWHRVTFYRKLAEIAGEYLKKGRPVYVEGRLETRKWTDKSGVERYTTDIIAS
                     DMKMLGSKPGSASFEAPDREEGGFAPSSSGSKAPPRSSSGFDDMDDDIPF"
     gene            complement(1928..3313)
                     /locus_tag="VFI43_09955"
     CDS             complement(1928..3313)
                     /locus_tag="VFI43_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7062489.1"
                     /translation="MSHSALPDKMSAAEKRATAGLASIYGLRMLGMFIILPVFAFYAE
                     HLPGGSDYTLIGVALGAYGLTQALLQIPFGWLSDRYGRKPVIYGGLILFAIGSFVAAS
                     AGNIYWVIFGRIIQGAGAISAAVMALAADLTREEHRTKAMAAIGMTIGATFALSLVIA
                     PMLNRLIGVPGIFVMTGVLALLAMAVVSRVVPDPIVSRFHSDTEASAGRFHNVLRNGE
                     LLRLDFGVFALHATLMALWLVVPLSLRGAGLAADEHWQVYFPVLLLSMVLVVPAIIYG
                     EKKAKLKQVFVISVAVLLVSQAMLAYSFDSLWGMALALLVFFAAFNLLEATLPSLISK
                     IAPVGAKGTAIGVYSSVQFLGTFVGASVGGYLFQHYGSTALFAFCGALLLSWLLFAIT
                     MKAPAAVRTRMYHVEKMDTGKANGLSRELASLPGVHEALVLASEGVAYLKVDMRGFDE
                     QGVVQLLGGKA"
     gene            3446..4615
                     /locus_tag="VFI43_09960"
                     /pseudo
     CDS             3446..4615
                     /locus_tag="VFI43_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381527.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
BASE COUNT         1076 a         1309 c         1193 g         1037 t
ORIGIN      
        1 ttgggcatgc gaaaaatcag gcaggaaaaa ctccggtgga tagatagcga tcacctcggt
       61 cgcacacaat cgatacgatc acggcgttgt ggacctccgc ggaaatcttc agggctgcgg
      121 ccagagcacc acctgatgaa atgccggcaa aaatcccctc ctctcgcgcc agccgccggg
      181 tcatatcctc agcttccgcc tgtgacacgc gcatcagaca gtcaacacgg ctggaatcat
      241 atattttggg aagatacgct tcaggccatt tacggattcc cggaatctgc gcgccctcct
      301 ccggctgcac gccgataatc tggatttccg gctttttttc cttgaagtat ctggaacaac
      361 ccattatcgt gccggtcgtg cccatgctgc tcacaaaatg cgtgatcgtc ccttctgtat
      421 ctcgccagat ctcggggcct gtgccttcgt agtgcgccct gggattatcg gggctcgcaa
      481 actggtcaag gatgactccg cgcccctcgt cacgcatccg ctcagccaca tcgcgcgcct
      541 cttccatgct gccctctttc gaagtcagta tgatttccgc cccgtaagcc ctcatggatt
      601 gccgccgttc tatgctgaga tgctctggca tgatcagtat gagcggatag cccatgattg
      661 cggcagccat cgccaatgcg atccccgtat tgccactcgt tgcctctatc agcgtatcac
      721 ccggcttgat gtccccgcgt tcctgcgccc gggtgatcat ggataaagca ggccggtcct
      781 tcaccgagcc tgccggatta ttcccttcca gcttaacgag tatggtgttg ctggtaattc
      841 ccggcaaatg cttgagtttc acgaggggcg tgttgccgac gaaatcttct atggttttaa
      901 acatgagagc taattcaaaa atggtggcaa cggtaaagga taattacgaa agccgagatc
      961 tgttgaaagc cgaaatatct gtttcaggcc ggattagtgg actgtaattt ccgtagcgtg
     1021 ggtagagcga agcgtaatcc ttcaaaacga ttgtgggttt cggtcacagg gcctccaccc
     1081 acgctatgac tttgcggcta ccggacaggc aatttgtttt cctttctggc cgccgcagcc
     1141 ccactcccga ccgtaatatc cttgcgcacc cgcttcatga tagccggacc tatgccattc
     1201 actttctcca gatcgtcggg cgacttgaac ggtccgtttt tcttacgata ttcgatgacc
     1261 gccttggctt ttgccgggcc aattccctgg agcgtttcca gctcggcctg ggtggctgtg
     1321 ttgatgttta cccccgcaag ggccgaacca aaaaatgcca aaaggatgat gaaaagtaat
     1381 tttttcatgc ccctgcccgg cctacggtag cgccgctatt tcagaagcaa gcgctcttga
     1441 gatcactctc actctttcaa ctgttggatc agaacggaat atcatcatcc atatcatcaa
     1501 acccgctgct gctccggggc ggagccttgc tgcccgatga actgggtgca aaaccgcctt
     1561 cttccctgtc cggcgcttca aacgaggcgc tacccggttt gctgcccagc attttcatgt
     1621 cgctcgcaat aatgtcagtg gtatagcgct ccaccccgga tttatcggtc catttacggg
     1681 tctccaggcg tccttcaaca tagactggac gacccttttt caggtattca cccgctattt
     1741 ctgccagttt gcgatagaac gtcacgcggt gccattcggt tttttcctgc ttttcgccgt
     1801 ttttatcctt ccaggtttcc gtggttgcca cggtaatatt agtcactgcc tcgccattgg
     1861 gcatataacg ggtttccgga tccttgccca ggttaccgat aaggatgact ttgttgactg
     1921 atgccattta tgccttacct cccaataatt gaacaacacc ttgctcgtca aatcctcgca
     1981 tgtcgacctt caggtaagcc accccctcgc ttgccagcac aagtgcctca tggacaccgg
     2041 gcagtgatgc cagttcacgt gataatccgt tggcttttcc ggtatccatc ttctccacgt
     2101 ggtacatccg cgtacgaacg gcagcgggcg ccttcatggt aatggcaaat aaaagccagg
     2161 acaggaggag cgcgccgcag aacgcgaaca gcgcagtact gccataatgc tgaaaaaggt
     2221 aacctcccac gcttgcgccg acaaacgtac cgaggaattg cacgctgcta tagacgccga
     2281 tagccgtgcc cttggcaccg accggggcga ttttcgagat gagcgacggc aaagttgctt
     2341 ccagcaggtt gaaagcggcg aaaaatacca gcagtgcgag tgccatgccc cataacgaat
     2401 caaatgaata cgccagcatt gcctggctca caagcagcac ggcaaccgaa atgacaaata
     2461 cctgcttgag ttttgctttt ttttccccat atatgattgc aggaacaacg agcaccatcg
     2521 agagaagcaa tactggaaaa tagacttgcc aatgctcatc cgccgccaac cccgccccac
     2581 gcagcgacaa cggcacaacc agccatagcg ccattagcgt tgcgtgaagg gcaaaaacac
     2641 caaaatccag gcgaagcagt tcgccgttgc gaagcacatt gtgaaaccgg cccgcggacg
     2701 cttcggtatc ggaatgaaag cggctgacga ttggatcggg aacgactctt gagacgacgg
     2761 ccatggccag cagtgccagc acgcctgtca ttacaaaaat tccggggacg ccgatcagtc
     2821 ggttcaacat gggcgcaatc acgagcgaaa gcgcgaacgt ggctccaata gtcatcccga
     2881 tcgcggccat cgcctttgtg cgatgttcct cacgcgtcaa gtcggcagcg agcgccatca
     2941 cagcagccga aatcgcgccc gccccctgga tgatcctgcc aaaaatgacc caatatatat
     3001 tgccggcaga ggctgcgacg aagcttccga tggcgaaaag gatcaagcct ccgtaaatga
     3061 ccggctttcg accgtagcga tcagacagcc agccaaaggg gatctggagt agcgcttggg
     3121 tgagtccata cgccccgagt gcaacgccaa tgagtgtgta atcacttccg cccggcaaat
     3181 gctcggcgta aaacgcaaat accggaagga tgataaacat gcccagcatg cgcaacccgt
     3241 aaatgctcgc aagcccggcg gtagcgcgct tctccgcggc agacattttg tcgggcaaag
     3301 ctgaatggga catcaatcgg ttgggaaaaa ggaggaaagt tgcgtatatt agcaggtttg
     3361 accaatgctc agatagcctc aggcagatct gccgggcctc ttacgctcaa ctcggcccac
     3421 cgggccaaag ctatcgaacc aaccaatgga actcataaga attcgcggcg cgcgcacgca
     3481 caatctcaag aacatcaata ttgatctgcc gcgaaacaag ctcgtcgtca tcaccggcct
     3541 gtcggggtcc ggaaaatcgt cgcttgcgtt cgatacgctt tatgcagagg gtcagagacg
     3601 gtatgtcgag tcgctctccg cctacgcgcg gcagttcctc cagttgatgg aaaagccgga
     3661 cgttgatctc atcgaagggc tatcacctgc catagcaatc gagcagaaag caacgtctca
     3721 caatccccgt tcaaccgtgg gcacggttac tgaaatacat gattacatgc ggctgctgtt
     3781 cgcgcgcgtg ggcgatcccc agtgcccgac ccacggcatt acgcttgctg cgcagagtgt
     3841 ttcccaaatg gtggattatg tgctgcagct gccaccggaa acccggctca tgatccttgc
     3901 gcctatggtt gtgggacgca agggcgaaca gatggagtta atcgatgaat ttcgggcgca
     3961 agggttcata cggctgagaa tcgatgggaa ggtgcatgaa atcgaggatc tccccaagct
     4021 gcaaaaaaac aaaaagcata cgatcgaagt agtgatcgac aggctgaagg tttcgcctga
     4081 ctccaagcag cgcctggccg agtcttttga aaccgcactg cgtcatgctg acggacgcgc
     4141 gttggcagtg gagatggatt caggtgtcga gcacctgttt tctgccaaat tcagctgtcc
     4201 ggtttgcagc tactcattgc cggagctcga accccggctg ttttccttca ataatccgat
     4261 gggagcgtgc ccgaggtgcg atgggttggg aaaaataact tttttcgatc ccaagcggat
     4321 tgtggcgttc ccgcatttat cgctcgcttc gggcgggatc aagggatggg acaagcgcaa
     4381 tcagttctat tatcagatgc tgtcgagcct cgctaatcac tacgggttcg acctcgaaat
     4441 tcctttcgag caactggcca tcccgatcca ggacgttatt ctgaatggct caggaaagga
     4501 aaaaatcacg ttcgcgtatt tgaacgagaa tggcacatga agctaccgga agcacccgtt
     4561 tgagggaatt gttcccaata tggaacggcg ctacagggag acggaatcgg ttacc
//
LOCUS       DASSHJ010000258        12324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_428080, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000258 DASSHJ010000000
VERSION     DASSHJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 12324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12324
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428080"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1687)
                     /gene="recN"
                     /locus_tag="VFI43_09965"
     CDS             complement(2..1687)
                     /gene="recN"
                     /locus_tag="VFI43_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7062490.1"
                     /translation="MLKFLSIRDFVIVDRMELEFAPGFTVLTGETGAGKSILIDALSL
                     VLGERSDAANVRNGCERTEISAEFDIKALPGLALWLRENGLESEDSDERICLIRRLVD
                     VNGRSRSFINGCSATLQQLRTAGEKLVDIQGQHAHQSLLRADAQRDLLDAFCGTRELA
                     QQVGEAYRHWQELRKRRADWEGRSDVFNQERERLQWQVNELSALNFSVDEWEALQADH
                     SRLSHAASLLEAAQAGSEALSESEFSSLAQVNSVISRLNHLLDYDQGLKPLLEILDSA
                     QIQLQEAAYELGRYQQRLDLDPQHLREVEERLAAIHTAARKYRVSPVELADLLQEATK
                     RLAELDQQEDDEVSAKEEETSLNRYLELARELSAKRTEAAIRLGQEVTAAMQTLAMEG
                     GEFNIALTPLESGNAGGMEQIEFQVAAHRGLPLRPLAKVASGGELSRIGLAIHVITSK
                     LGAAPTLIFDEVDVGIGGRVAEIVGEMLKKLSGQRQILCITHLAQVASAADNQWLITK
                     TAGSSGGDGTISSRITALNKEERIEEIARMLGGVKITETTRRHAAEMLFASQK"
     gene            complement(1695..2582)
                     /locus_tag="VFI43_09970"
     CDS             complement(1695..2582)
                     /locus_tag="VFI43_09970"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET7062491.1"
                     /translation="MPSSRFKTVALIGKYKNNDIIPPLLRLGRYLRERDVEVILDCLT
                     ASNVADNEWPVMDLEEIGTEVDLAVVLGGDGTMLNIARKLAPRNVPLVGVNQGRLGFL
                     TDLSIENMVETLGSMLDGQYIEERRMLLCGEVSRENSVLFSALALNDVAVSRGIGGNM
                     IEFEVRINGEYVYSLRSDGLIVATPTGSTAYALSSGGPILHPSLELIALVPVSPHTLS
                     NRPIVVGPDAIVEILMHRTSVARVHSDSHSHFDLQEYDNVIVRRSPYRATLLHPSDHS
                     YYRVLREKLGWSGLPRNIA"
     gene            2811..3830
                     /gene="hrcA"
                     /locus_tag="VFI43_09975"
     CDS             2811..3830
                     /gene="hrcA"
                     /locus_tag="VFI43_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7062492.1"
                     /translation="MLNERAKILLKTLVERYISEGQPVGSRSLSKSSGLDLSPATIRN
                     VMADLEEMGFVASPHASAGRIPTHRGYRFFIDTLLVVKPLDLMEIHQLEDNLPPDNPS
                     RLFNSASQLLAELTRFAGVVVAPKRSSAVFRYIEFMALSEKRVLLIIVTPEGDVQNRV
                     LFTDRAYSQVELIEAANFINQNYAGHAIDDIRTRLQNELKQLRRDVTTLMAAAIEAGD
                     EAIREHSGAVVVAGERKLLDVQDISSNMSTLKKLFDLFERKTALLQLLEFSRKGEGVQ
                     IFIGDESGVVTLDEFSVVTAPYEVDGKVVGTVAVIGPTRMAYERVIPVVDVTARLLSS
                     ALSQH"
     gene            3898..4992
                     /gene="hemH"
                     /locus_tag="VFI43_09980"
     CDS             3898..4992
                     /gene="hemH"
                     /locus_tag="VFI43_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET7062493.1"
                     /translation="MAIVISPETPGQQGTPSQVSVLLINLGTPDAPTPEALRPYLKQF
                     LSNPRVVEFPRWAWWPILNGVILNTRPRKSAEKYAQIWMPEGSPLKVHTVRQTELLRE
                     ELAKRVEAAPIVEYAMSIGNPSIGAVLGKMKERGCEQILILPLYPQYASSSTASAYDE
                     IFAQLSRMRNVPALRTVKHYHDDPGYIASLAQNIRDYWSENGNPDKLVMSFHGVPRST
                     LDKGDPYHFECQRTGRLLAKALTLDESDYRICFQSRFGRAEWLKPYTAATLEQLGAQH
                     VGRVDVVCPGFVSDCLETLEEIAMEGKTLFMRAGGKAFHYIPCLNERRDWICALADIA
                     LDNLNGWVDVQDAEKGASRMAQPLPQGASA"
     gene            complement(5081..5728)
                     /locus_tag="VFI43_09985"
     CDS             complement(5081..5728)
                     /locus_tag="VFI43_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7062494.1"
                     /translation="MEILATFVDIVLHLDKHLIWLVQNYGDWVYLILFLIIFCETGLV
                     ITPFLPGDSLLFVAGAIAATGAMEVQWLATLLMLAAFSGDNTNYWIGRYFGPRIFKNS
                     SSPLLNRAHLQKTHEFYEKHGGKTIIFARFLPIIRTFAPFVAGIGRMIYPRFIAYSAF
                     GSVFWISFFVFGGYFFGNVPVVKNNLTFFIFAIIIISVLPGFIQFMRSRFSGQGK"
     gene            complement(5787..6716)
                     /gene="secF"
                     /locus_tag="VFI43_09990"
     CDS             complement(5787..6716)
                     /gene="secF"
                     /locus_tag="VFI43_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7062495.1"
                     /translation="MEFFRIKRDIPFMSWGKYTTTISLVTFIAALFFLYNKGLNLGVD
                     FTGGTVMEVGYSQPADINRIRATLLKLGMPDVSVQNFGTSRDVLIRLPARPDLSSARL
                     SDTVIAALRQENQDAEIRRVEFVGPQVGQELVESGALALLIVSLGIIAYLAMRFEWKF
                     GVAGVIANLHDVVIILGFFAFFQWEFSLTVLAAILAILGYSVNESVVVFDRIRENFRK
                     MRKATVAQVIDNAITRTMSRTIITHGCTQLVVTAMLFFGGEALHYFALALTIGILFGI
                     YSSVLVASPIVMLLGVSREDLVKPEKKEAEAMP"
     gene            complement(6755..8635)
                     /gene="secD"
                     /locus_tag="VFI43_09995"
     CDS             complement(6755..8635)
                     /gene="secD"
                     /locus_tag="VFI43_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7062496.1"
                     /translation="MNRYPFWKYLIIGVSILLGLLYTLPNFYGESPAVQISPLRTAAK
                     ADTALLQRVEDALKKSGIASNGIFLDAGGVKVRFTDTDAQIKARDMLQATLGNGYMVA
                     LNLLSNSPQWLSSIGALPMYLGLDLRGGVHFMLQVDMKGALSKALDRFGADIRGTLRE
                     KKIPYAGLDRQASQIVVKFRNAESRAKGEAEINKTYTDLNIREDNAGTEFHLIATLKP
                     EAQKRIQDSAVQQNITTLRNRVNELGVAEPIIQQAGADRVVVQLPGVQDTAKAKDILG
                     RTATLEVRMVDEEHDVQDALRGQVPFGSELYTERGGGPLLVKKQVVLTGDRINDAQAG
                     FDNNNQPAVHLNLDNNGARIFRQLTHDNVGKRMAILLIEKNQAEVITAPVIREEIGGG
                     RVQISGRMTTEEARDVALLLRAGALAAPMDIIEERTVGPSLGADNIARGFNSTLFGFA
                     AIAVFMTVYYLVFGFISVLALGVNLLLLVGLLSILQATLTLPGMAAIALTIGMAIDAN
                     VLINERIRDELRQGMPPQGAINAGYERAFGTILDSNITTLIAGIALFAFGSGPVKGFA
                     VVLCLGILTSMFSAVMVSRGLVNLVYGGRRKLERVSIGQVWKPVDKRPVDKSVSSRAS
                     KA"
     gene            complement(8718..9053)
                     /gene="yajC"
                     /locus_tag="VFI43_10000"
     CDS             complement(8718..9053)
                     /gene="yajC"
                     /locus_tag="VFI43_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7062497.1"
                     /translation="MLISEAFAQAAATPQPGADFMSLLPLIAIFAVFYLLLIRPQAKR
                     AKEQKLMIDALQKGDEVTTSSGELGRIVKVSGNYVILEIAENVRVTMLKSSVQTLLPK
                     GTLNSIEKE"
     gene            complement(9094..10200)
                     /gene="tgt"
                     /locus_tag="VFI43_10005"
     CDS             complement(9094..10200)
                     /gene="tgt"
                     /locus_tag="VFI43_10005"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7062498.1"
                     /translation="MEFKLHRTEGLARRGTLTLSHGVVETPAFMPVGTYGAVKTMSPA
                     DLRGVDAHVVLGNTFHLWLRPGLEVIEAHGGLHRFMGWERPILTDSGGFQVFSLGVLR
                     KISEEGVKFRSPVNGDACFLTPEESMHIQRVLNSDVVMIFDECTPYPADERAAESSME
                     LSLRWAERSRRAHDDRRNTNALFGIVQGGMYETLRDRSLSGLLDVGFDGYAIGGLSVG
                     EPKDDMERILKHTCPQLPADKPRYLMGIGTPADIVHAVAQGVDMFDCVLPTRNARNGW
                     LFTRNGIIKLRNNQYRLDTSPPDEQCGCYTCGHFTRAYLHHLQRTGEMLGARLNTLHN
                     LYYYQQLMGEIRSSIEADRFETYAQQFLGHGTSC"
     gene            complement(10632..11660)
                     /gene="queA"
                     /locus_tag="VFI43_10010"
     CDS             complement(10632..11660)
                     /gene="queA"
                     /locus_tag="VFI43_10010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7062499.1"
                     /translation="MKIQDFDFVLPPELIAQFPAEKRASSRMLHLEGSTGALGDAAFA
                     DLPKYIRPGDVMVFNDTRVLKARLHGVRDTGGRAEVMVERVLGRHVVQAVIRSSHPPK
                     PGSRLFLHECIEVVVLERVEDFYTLKFEHESNVIELLERFGTLPLPPYITRAVTPADE
                     ARYQTVYGKEPGAVAAPTAGLHFDEHMLETLKQMGVVMANVTLHVGLGTFQPVRVENI
                     AEHKMHSEIFNVPAETVESITRARAGGGRVLAVGTTSLRALEAAAAGGKLKARRGETD
                     IFLTPGYRFQVVERLLTNFHLPRSTLLMLVSAFGGVENIRNAYRHAVDQRYRFFSYGD
                     AMLIERNA"
     gene            11779..11865
                     /locus_tag="VFI43_10015"
     tRNA            11779..11865
                     /locus_tag="VFI43_10015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11813..11815,aa:Leu,seq:caa)
     gene            12028..>12324
                     /locus_tag="VFI43_10020"
     CDS             12028..>12324
                     /locus_tag="VFI43_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HET7062500.1"
                     /translation="MAAELLSDVEIRNAKAGNKPRKLRDGNGLNLLIHPNGSKYFQLR
                     YTLHRKEKTLQLGVYPVISLAEARVAAKAARQLVANDVDPVLNKRVQAARQAASA"
BASE COUNT         2878 a         3469 c         3240 g         2737 t
ORIGIN      
        1 gctacttctg gcttgcaaat agcatttccg ccgcatgcct gcgcgtcgtt tctgtgattt
       61 ttaccccgcc tagcatgcgt gcaatttcct cgatccgctc ctccttgttg agggcggtaa
      121 tccggctcga tatcgtgcca tcgccccccg acgaaccagc tgttttcgta atcaaccact
      181 gattgtctgc tgctgaagca acctgggcga gatgggtgat gcataggatc tggcgctggc
      241 cgctcagttt tttaagcatt tcaccgacga tttctgccac tcgcccgccg atcccgacgt
      301 caacctcatc aaatatcagg gtgggtgctg cgccaagctt gctcgtgatc acgtggatgg
      361 ccaggccaat acgagacagt tctcctccgg atgcgacctt tgccaacggt cgcaagggta
      421 agccccgatg tgcggccact tgaaattcga tctgctccat tccgcccgca tttccactct
      481 caagcggagt tagcgcaatg ttaaattctc ctccttccat cgccagcgtc tgcatcgcgg
      541 cggtgacttc ctggccaagc cgtatggcgg cctcggttct tttggcgctc aactctctgg
      601 caagctcgag atacctgttt aaggaagttt cctcttcctt tgccgatact tcgtcgtctt
      661 cctgctgatc aagttctgcc aatcgttttg tagcctcctg caacagatcc gccagttcaa
      721 caggcgaaac gcggtacttc cttgcagcag tgtgtatcgc tgccaatcgt tcctcgactt
      781 cacgcaagtg ctggggatca agatcgaggc gctgctggta tcgcccgagt tcgtacgcgg
      841 cctcctgcaa ctgaatctgc gcggagtcga gtatttccag caaaggcttg agcccttggt
      901 cataatcgag caagtggttc aggcgcgaga tgactgagtt gacctgcgca agcgacgaga
      961 attcgctttc ggacagcgct tccgatcccg cttgcgcagc ttcgagcaag cttgcggcat
     1021 gagaaagacg gctgtgatcg gcctgcagcg cctcccactc atcgacggaa aaatttagcg
     1081 cggaaagctc gttcacctgc cattgcaaac gctcgcgctc ctggttgaac acatcggagc
     1141 gtccttccca gtcagcacgc cgtttccgca actcctgcca gtgccgatac gcttcgccta
     1201 cctgttgcgc cagctcgcga gttccgcaaa aagcatcaag aaggtcacgt tgagcatctg
     1261 ctcgcagcaa tgactgatgt gcatgctgac cctgaatgtc caccagtttc tctcccgctg
     1321 tgcgcaattg ctgcaacgtt gctgaacaac cattgataaa gcttcgcgac ctcccgttaa
     1381 catccacgag gcggcgtatc aagcagatgc gctcatcgga gtcttcgctc tcaagaccat
     1441 tttcccgcaa ccagagagcc aagccgggca acgccttgat atcgaactca gcacttattt
     1501 cggtgcgctc acacccgttg cgcacattgg ctgcgtcgct gcgctcgcca agaacaagcg
     1561 ataacgcgtc gatcaaaata gatttgcctg cgcccgtctc cccggtaagc acggtaaaac
     1621 cgggggcaaa ttcgagctcc atgcggtcaa caatgacaaa atcccgaatg ctgagaaact
     1681 ttagcattgg cagctcacgc gatgtttcgc ggcaagccgc tccagcccag cttctcccgt
     1741 agtacccgat agtagctgtg gtcggaagga tgcaacagcg tggccctgta tggggaacgc
     1801 ctgacaatga cgttatcgta ctcctggagg tcgaagtgcg aatggctgtc cgagtgcacg
     1861 cgcgccacgg atgtgcgatg catcaaaatt tccacaatgg cgtccggccc taccacgata
     1921 gggcggttgc taagcgtatg cggactgaca ggtacgaggg caatcagctc caggctgggg
     1981 tgcaggatgg ggccccccga agataatgca tatgctgtgg atcctgtcgg agtcgccacg
     2041 atcaaaccgt cagagcgcaa ggagtagaca tactccccat tgatccggac ctcgaattcg
     2101 atcatatttc cgcctattcc ccggctcacc gctacatcgt ttaaagcgag agcactgaac
     2161 aagacgctgt tctcacgcga aacttcaccg caaagcagca tgcggcgctc ttcaatatat
     2221 tgcccatcga gcatggagcc cagcgtttcc accatattct cgatcgaaag atcggtgaga
     2281 aatccgaggc gcccctggtt gaccccgacc aggggaacgt tgcgtggcgc aagcttgcgg
     2341 gcgatgttga gcattgtgcc gtcgccgccc aacacgacgg ccaggtccac ctccgtgccg
     2401 atctcctcca ggtccatgac cggccattcg ttatcggcga cattggaagc agtaaggcaa
     2461 tcaaggatta cttccacatc cctctcccgc aaatatctcc ccaatctcag caatggtggg
     2521 attatgtcgt tatttttata ttttccgatt agcgctactg ttttaaatcg ggaactcggc
     2581 atctcgggat ttaatctcaa tctgatagga gttagcgacc agcgcctgtt tccaaaaaaa
     2641 cgtactcaag attaaaccat atcggccgat aggtgacaaa gcattcggat gaatccttga
     2701 atcctgaaaa cacacattga aaaacgtcag caaaagttgc atcggttcaa gttattgccg
     2761 ggccggaagc tcaaccggtt taaaattcaa tcctgttgta caatatcccg atgcttaatg
     2821 aacgtgcaaa aatattgctg aagacgcttg ttgaacgcta tatcagcgaa ggacagccag
     2881 tgggttcccg ctcgctgtca aaatcctccg gacttgatct cagcccagct accatccgta
     2941 acgtcatggc tgatctggag gagatggggt ttgttgccag cccccacgct tccgccggaa
     3001 gaataccgac tcatcgaggc taccgttttt tcatcgatac actgctggtc gtcaagcctc
     3061 tcgatctcat ggaaatccac caactggagg ataacctgcc ccctgataat ccttcgcgcc
     3121 tttttaactc ggcttcacaa ttgctcgccg aattgacccg ctttgcgggc gtggttgtcg
     3181 ccccgaaacg cagcagcgca gtcttccgct atatcgagtt catggcgttg tcagagaagc
     3241 gtgttctgct tatcatcgtg acgcctgagg gcgatgttca aaaccgtgtg ctttttaccg
     3301 accgggcata cagccaggtt gaattgatcg aagcggcaaa ctttatcaat cagaactatg
     3361 ccgggcacgc aattgacgac attcgaaccc ggctgcagaa tgagctgaaa cagctgcgcc
     3421 gggatgtgac aacgctgatg gcggcagcca tcgaagcggg cgacgaagcc atccgtgaac
     3481 atagcggagc agttgtcgtg gcaggggagc gcaaattgct ggatgtgcag gacatttcca
     3541 gtaatatgtc gacattgaaa aaattatttg atcttttcga gcgcaagacg gcgttgctgc
     3601 aattgctgga gttcagccgc aaaggcgaag gtgtgcagat ctttattgga gatgaatctg
     3661 gcgtggtcac gctggatgag tttagcgtgg ttacggcgcc ttatgaagtg gatggaaaag
     3721 tcgtgggaac cgtggcggtg atcggtccga cccgcatggc ctatgagcgt gtcattccgg
     3781 tcgtggatgt cactgcccgg ctgctctcca gcgcgctgtc ccagcattga gccaccgagc
     3841 tgctgctctt ccgttacttg atccgccgtt tgccatggaa acggccacca gcccgagttg
     3901 gctatcgtga tttcgccgga aacacccggt cagcagggaa cccccagcca ggttagcgta
     3961 ctgctgatca atcttggcac ccccgacgca cctactccag aggccctgcg gccttacctc
     4021 aagcaatttc tgagtaatcc tcgggtcgtg gagttccccc gatgggcctg gtggccgatc
     4081 ctgaacgggg tcatactgaa tacccgccca agaaaatcgg ccgagaaata cgcccagata
     4141 tggatgcctg aagggtcgcc attgaaggtg cacactgttc gccagaccga gcttttacgt
     4201 gaagaactgg cgaagcgcgt tgaagccgcc cccattgtag aatatgcaat gagcatcggc
     4261 aatccgtcta tcggcgctgt gcttgggaaa atgaaagagc ggggatgcga acagattttg
     4321 atactgccgc tttaccctca atatgcatcc agcagtacgg cttcggcata tgacgagata
     4381 ttcgcgcaac tgtcgcggat gcgtaatgtc cccgcattgc ggaccgtgaa gcattatcat
     4441 gacgaccccg ggtacatcgc ctcgcttgcg caaaacattc gcgattattg gagcgaaaac
     4501 ggcaatcccg acaaactggt gatgagtttc catggagtgc cgcgctccac gctcgataag
     4561 ggcgatccct atcacttcga gtgccagaga accggaagat tgctggccaa agcacttacc
     4621 ctggatgaaa gcgattaccg gatttgcttc cagtcccgct ttggccgggc agaatggctg
     4681 aagccatata cagcggccac actggaacaa ctgggtgccc agcacgtcgg acgagtggat
     4741 gtcgtctgcc ctggtttcgt atccgattgc ctcgaaacgc tcgaagaaat cgccatggaa
     4801 ggcaaaaccc tgtttatgcg agcgggtgga aaggcgtttc actatatacc ttgcctcaac
     4861 gaacgccgcg actggatatg tgcgctggct gatattgcgc ttgataatct gaatggctgg
     4921 gtggacgtgc aggatgcgga gaagggagcg agtcgaatgg cccaacctct cccccaaggt
     4981 gccagtgcgt aataccaagg ctgggcgcgc caggcttaac tcccctatcc aggagtggga
     5041 gagggatcaa aaccacctta ggaagccgga ttgtggctta ctactttccc tgaccactaa
     5101 accggctgcg catgaactgg ataaaaccgg gcagcactga aatgataatg atcgcgaaga
     5161 tgaagaaggt tagattgttt ttcaccacag gcacgttgcc gaaaaaatag cccccgaaga
     5221 caaaaaaact gatccagaaa acactgccga acgcgctgta cgcgatgaac ctgggataga
     5281 tcatgcgccc gataccagcg acgaatggcg caaaggttcg aataatcgga agaaaccggg
     5341 caaatattat cgtcttgccc ccgtgctttt catagaactc atgcgtcttt tgcaaatgag
     5401 cgcgattgag caaaggagag ctggagttct tgaatatcct ggggccgaaa tagcgcccga
     5461 tccagtaatt cgtgttatcc cctgagaatg ctgcaagcat gagcagcgta gcgagccatt
     5521 gaacttccat cgctccggta gctgcaattg cacccgctac aaacaagagt gaatcgccag
     5581 gcagaaatgg ggttatcacg agcccggttt cgcagaaaat gatcaggaac aggatcaggt
     5641 aaacccagtc gccataattc tgaaccagcc atatgagatg tttatccagg tggagaacga
     5701 tgtcgacaaa tgtggcgaga atttccaaga ttatgatcgg atagattgag ggggagatac
     5761 ggaaggagga aggccggcga tctcgatcag ggcattgctt ctgcttcttt cttttccggc
     5821 ttgacaagat cttcgcgaga gacacccagc agcatcacga tggggcttgc caccagcact
     5881 gaggagtaaa ttccgaagag aatgccgatg gtcagcgcga gcgcaaagta atgcagcgcc
     5941 tcccctccaa agaacagcat ggcggttaca accagttgtg tacaaccatg ggtgatgatc
     6001 gtgcgtgaca tggtgcgagt aatggcgtta tctatcactt gcgcgaccgt tgccttgcgc
     6061 attttgcgaa agttttcccg tatccggtcg aataccacca ccgactcatt caccgagtag
     6121 ccgaggattg caaggatcgc cgccagcacc gtgagcgaga actcccattg gaaaaaggca
     6181 aaaaacccaa gaatgattac gacgtcgtgc aggttggcga tgacgcccgc gacgccgaac
     6241 ttccattcga accgcattgc aaggtatgca atgatgccga gcgaaacgat cagcaatgcc
     6301 agggcgccgc tttcgaccag ctcctggcct acctggggcc cgacaaactc gacccgcctt
     6361 atctcggcgt cctggttttc ctggcgaaga gccgctatta ctgtgtccga cagccgggcg
     6421 ctggaaagat ccgggcgggc cggaaggcgt atcaacacgt ctcttgacgt tccaaagttc
     6481 tgcacgctta cgtcgggcat tccgagcttc agaagggttg cccgtatcct gttgatgtcc
     6541 gcaggctgcg aataaccgac ctccatgacg gtaccgcccg taaagtcgac cccaaggttc
     6601 aatcccttgt tgtacagaaa aaataatgcg gcaataaacg tcaccagcga aatcgtcgtg
     6661 gtgtactttc cccagctcat gaagggaatg tcgcgcttga tcctgaaaaa ttccatgctg
     6721 gcccttcgcc tgaaaattgt tcttaaaaag aacgctaggc tttgctcgcc cttgaactca
     6781 cacttttatc taccggcctt ttatccactg gcttccatac ctgaccgatc gagactcgtt
     6841 ccagcttccg gcgtccgcca tagacaagat tgaccagtcc gcgtgacacc atgaccgcgc
     6901 tgaacatcga tgtgagaatc cccaggcaca gcacaacggc aaagcctttg acagggcccg
     6961 aaccaaaagc aaacaaggca atgcctgcaa tcagggtggt aatgttcgaa tcaagtatgg
     7021 tgccaaacgc ccgctcgtac cccgcgttga ttgcgccttg aggcggcatc ccctgcctca
     7081 gttcgtcacg gatacgctcg ttgatcagta cattcgcatc gatagccatg cctatggtaa
     7141 gcgctatggc tgccatgccc ggcagcgtca gggtggcctg taaaatggaa agcagaccca
     7201 ccagaagcag caggttgacg ccgagcgcca gtaccgaaat aaagccaaac accaagtagt
     7261 atacggtcat gaagacggcg atcgcagcaa aaccgaacag tgtggaattg aaaccccggg
     7321 caatattgtc tgccccaagg ctggggccaa ccgtgcgctc ttcgatgata tccatgggtg
     7381 ccgccagcgc tcccgcccgc agcaaaagcg caacgtccct cgcttcttcg gtcgtcatgc
     7441 gcccgctgat ctgcacccgt cccccaccga tctcttcacg gatcaccggc gccgtaatga
     7501 cctccgcctg gttcttctcg atcagcagga tggccatgcg tttccctacg ttgtcatggg
     7561 tcagctgccg gaaaatgcgc gcgccattat tatcgaggtt gaggtgcacg gccggctggt
     7621 tgttgttatc aaaaccggct tgcgcatcgt tgatgcggtc gcctgtgagc acgacctgct
     7681 tcttcacaag taacggccca ccgccccgct cagtgtaaag ttcagagcca aatggaacct
     7741 gcccgcgcag ggcgtcctgc acgtcatgct cctcatccac catccgcact tccagcgtgg
     7801 ctgttcgccc caggatatcc ttggctttgg ccgtatcctg taccccaggc agttgcacca
     7861 cgacgcggtc cgctccggcc tgctgaataa tgggctcggc tacgccaagc tcgttcacac
     7921 ggttgcgcaa cgttgtaata ttctgctgta cggccgagtc ctggatacgc ttctgcgcct
     7981 ccggcttgag tgtggcgatc aaatggaact cggtccccgc attgtcttcc ctgatgttca
     8041 gatcggtata ggtcttgttg atttcagcct caccttttgc tctcgattca gcattccgga
     8101 atttcacgac gatttgggag gcctgcctgt ccaggccggc atagggaatt ttcttctccc
     8161 gtagagtgcc tcgtatatcc gcgccaaacc tgtcgagcgc cttggaaaga gcgcccttca
     8221 tgtccacctg cagcatgaag tgaacaccgc cgcgtaggtc tagccccagg tacatcggca
     8281 gagcacctat gctggacaac cattgcggag aatttgaaag caggttcagg gcgaccatat
     8341 acccattgcc gagggtggcc tggagcatgt ccctggcctt gatctgcgcg tcggtatcgg
     8401 taaaccggac tttgacccca cccgcatcga gaaatattcc attgctggca atgcccgatt
     8461 tctttagcgc atcctccacg cgctgaagaa gcgcagtgtc ggccttggcg gctgttcgca
     8521 gcggtgaaat ttgtacagcg ggcgattcgc cgtagaaatt gggaagcgta tagagtaatc
     8581 caagaaggat ggaaacccca ataatcaggt acttccagaa ggggtagcga ttcatgtctc
     8641 aggagtggaa acgaagggag cgaaaggaaa gaaggggtgg gaaggtgcgc ccttcgagtg
     8701 ttgctgtgaa attcgattta ttccttttca atactgttca gggttccctt cggcaggagc
     8761 gtctgaacgg acgatttcag catggtgacc cgcacatttt cggcaatttc aagaatgacg
     8821 taatttccgc tgactttgac gatacgtccc agttcgccgc ttgaggtcgt tacctcgtcc
     8881 cccttttgca gcgcatcgat catcaatttt tgttccttgg cgcgttttgc ctggggacga
     8941 atcagcagaa gatagaatac cgcgaagata gcgatcagcg gcagcaggct catgaaatca
     9001 gcacccggct gaggggtggc agcggcctgg gcaaatgctt cactaatcag catggcattt
     9061 ctccggtaaa aatttcaaaa gtaaacgatg attttagcat gatgtgccgt ggcccagaaa
     9121 ttgttgtgca taagtctcaa accggtcggc ttcgatcgat gaacgaattt ctcccatcaa
     9181 ttgctggtaa taatatagat tatggagggt attgagacgc gcacccagca tttcgcccgt
     9241 acgttgcagg tggtgcagat aagcgcgggt aaaatgaccg catgtatagc agccgcactg
     9301 ctcgtccggc ggcgatgtat cgagccggta ctggttgttg cgcagcttga taatgccatt
     9361 gcgcgtaaag agccagccgt tgcgcgcatt gcgtgtagga agaacgcagt cgaacatgtc
     9421 cactccttgt gccactgcgt gaacgatatc tgcgggcgtg ccaatcccca tcaggtatcg
     9481 cggcttgtcc gccggcaact ggggacaggt gtgcttcagg atgcgctcca tatcgtcctt
     9541 tggttcaccc acggaaaggc cgccgattgc atagccgtcg aaaccgacat ccagcaaccc
     9601 ggaaagtgaa cggtctctaa gagtttcgta catgccgccc tgaacaatcc cgaacaacgc
     9661 attggtgttc ctgcggtcgt cgtgcgctcg ccgcgagcgt tctgcccagc gcaagctcaa
     9721 ttccatcgag ctttcagccg cccgctcgtc agcgggataa ggcgtgcact catcgaatat
     9781 catgacgaca tccgagttga gcacacgctg gatatgcatc gactcttcag gcgtcaaaaa
     9841 gcaggcatcc ccgtttaccg gagatcggaa cttgactcct tcttcgctta tctttcgcag
     9901 cacccccagg ctgaatacct ggaagccccc cgaatccgtc aatataggcc gttcccagcc
     9961 catgaaacgg tgcaggcctc catgcgcctc gatcacctcg aggccgggtc gcagccataa
    10021 atgaaatgta ttgccgagaa cgacgtgagc gtccactcca cgcaagtcgg ccggagacat
    10081 ggttttgact gcgccgtaag tgccgacagg catgaatgca ggagtttcga caaccccatg
    10141 cgagagtgtc agggtgccac gacgcgctaa tccctcagtt cgatgcagct tgaattccat
    10201 ttggatcaga aggggtagat caggtagttt taagggtgga tcaggtagtt ctaatatagt
    10261 gatgaagcaa gatttaagga ccagcgagcg acgtacgggt aactgaccga cttgtagccc
    10321 caggaagtca tcaatgcctc atctctccca tttatgggaa agatgccagg agagagacct
    10381 tagtgcgcca ccctcgcctg tagccctgga accttgtcaa ctcctctctc ccattttatg
    10441 ggagagaggc cgggagagag ggcgaatttt ggaaacactc ctctccctcg ccctcgccca
    10501 cagtgggaga ggggaaagaa tctggtttca agcagcttgg gttgttgtga ttaataataa
    10561 gtctctgctg taaaaacaga gtcctattca ggtccttgag taagctataa aaaaatcaaa
    10621 tcaaaaaata atcacgcgtt ccgctcgatc agcatggcat ctccataact gaaaaaacgg
    10681 tagcgctggt caaccgcatg ccgatatgca tttcgaatgt tctcgactcc gccaaaagcg
    10741 gagacgagca tcagcaaagt cgagcgtgga agatgaaaat tggtcagcaa gcgttcgacc
    10801 acttggaaac gatatccggg agtgaggaag atatccgttt ctccccgcct ggcttttagc
    10861 ttgcctcccg ctgccgcggc ctccagcgca cgaagtgaag tagtgcccac ggccagcacc
    10921 ctgccccctc ccgccctcgc gcgcgtgatg gactccacgg tttcagccgg cacattgaaa
    10981 atctcgctat gcatcttgtg ctcggcaata ttttcgacgc gtacgggctg aaacgttccc
    11041 agtccgacat gcagagtaac attcgccatc accacgccca tctgtttcag cgtctcgagc
    11101 atgtgttcgt cgaagtgcaa cccggcagta ggggcagcca ccgctcccgg ctctttccca
    11161 tagaccgtct gataccgcgc ctcatccgct ggcgttaccg ctcgagtgat atacggcggc
    11221 agcggcaggg ttccaaaacg ctccaataat tcgattacgt tgctttcgtg ctcgaacttc
    11281 agggtgtaaa aatcctctac gcgctcaagc accaccacct caatacactc gtgcaggaat
    11341 agcctggagc ctggcttggg gggatggctt gagcggataa cggcctggac gacgtgtcgg
    11401 ccgagcacac gttcaaccat cacctccgcc ctccccccgg tatccctcac accatggagt
    11461 cgcgccttca atacccgggt gtcgttgaac accatgacgt ctcccggccg tatgtacttc
    11521 ggcagatcgg caaatgcagc gtcgcccagc gctccggtgg agccttccag atgcagcatc
    11581 cggctgcttg cccgcttctc ggcaggaaat tgggcgataa gctctggggg aagaacgaaa
    11641 tcgaaatcct gaattttcac cccaatatta tacccgcgtg tccataggtt cccattgcag
    11701 ccggggagat ttcaggcagg ttgccgcggc ctgggttttc agtaataatc gcgctggcga
    11761 aagaaaagcc ggtcccttgc cgggatggcg gaattggtag acgcacggga ctcaaaatcc
    11821 cgcgatcgcg agatcatggg ggttcgattc cccctcccgg caccatgaga taatccgagg
    11881 aagtccatta aagtaaaata atgctttaaa gtcaatccgt tttagtaaag acctgcgcca
    11941 tatggtattt tactgtccat tgacatccaa gtgtaagacg ggtaaaagaa cgggtaaatt
    12001 atttaccgga gattcttttt acccgctatg gccgccgaac tattatctga tgtagaaatc
    12061 cgcaatgcta aagcagggaa caagccgcgc aagttgcgcg atggaaatgg tctcaacctc
    12121 ctgattcacc ccaacggttc caaatacttc cagcttcgct acactctcca ccgcaaggaa
    12181 aaaacgcttc agctcggcgt ctaccctgtt ataagcttag cagaagcccg ggtagcagca
    12241 aaggcggcac gccaactcgt cgccaatgac gtagacccgg tgctaaacaa gcgcgtgcag
    12301 gctgcaagac aagcggcctc cgct
//
LOCUS       DASSHJ010000259         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_381537, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000259 DASSHJ010000000
VERSION     DASSHJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381537"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(470..799)
                     /locus_tag="VFI43_10025"
     CDS             complement(470..799)
                     /locus_tag="VFI43_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062501.1"
                     /translation="MKSAVLALLFALALSACASSKMTGLREGMTKAEVIKVAGDPDGF
                     QRSGDYEVLLYLERRTSAWSYFSGAIQDLVDYTVILKDDHVVEYGPGRAHQRESNSAP
                     FVRVPAR"
     gene            complement(857..1066)
                     /locus_tag="VFI43_10030"
     CDS             complement(857..1066)
                     /locus_tag="VFI43_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062502.1"
                     /translation="MNSTPYDTAEMLFAFHVSEKARAIREQHLMKFPEHMRETEKRRY
                     PLELAVKEVLAEIAEVAMLIKELES"
     gene            complement(1391..1945)
                     /locus_tag="VFI43_10035"
     CDS             complement(1391..1945)
                     /locus_tag="VFI43_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7062503.1"
                     /translation="MNFAKVIREAREKLNLNQSQLADLVGVSPQAVQQWESGATQPRG
                     KRLSKIAEVLKLSPTLMHFGIFTEAAESTPAKAVQEPKAAEYKKDRRAARSSAPEKPP
                     STSDPLIDEVVEAMRRMSVEDAARLVTISKALAGGARIEEAAVDLKPLPAPDLAPHPP
                     PAFPPRRLPGGNRTKARGKTAIGR"
     gene            2280..2432
                     /locus_tag="VFI43_10040"
     CDS             2280..2432
                     /locus_tag="VFI43_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062504.1"
                     /translation="MTSVEQQAVKAVAEQTMAGEIPMRINDFQEQQMMQAVRTCGYDK
                     KGQLDL"
     gene            2429..3097
                     /locus_tag="VFI43_10045"
     CDS             2429..3097
                     /locus_tag="VFI43_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062505.1"
                     /translation="MKNLSEGKGGRVVTRFRSGIAVAYMGAIVGVGNGEVQAGGPAST
                     GTVAGTGTSSAASAFVSSQQAQMQSQIQIQNNSQMQTSSSGATGGEGGSANNAGNSQV
                     VNLANSGVRNNPAIGLGLMVPTAPCRDTYSIGASAAGLGGALAWSKADRECRLQDIGA
                     AFIRAGLVDDGLALWCSIDGVDEIAPTCRRLKTASRKAPAVAGAPGMDQENASDEAVN
                     EFSW"
     gene            3166..3690
                     /locus_tag="VFI43_10050"
     CDS             3166..3690
                     /locus_tag="VFI43_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062506.1"
                     /translation="MKFRNPEHALRWAYETSNSPIVKISSIHAMRGADRGEGGLSKDE
                     LTPYDFHAQAALILSLCSSVLSPLHMAYVSIQFGREAAGFNLLGRQLAANFGTGMHSR
                     KGIEHVIRAYCGEKIGLRELRKTMSCGMLKAASFRNRGYDMLDAIHAQALNILRREME
                     SKGLVRCEDAREIA"
     gene            complement(3840..>4376)
                     /locus_tag="VFI43_10055"
     CDS             complement(3840..>4376)
                     /locus_tag="VFI43_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062507.1"
                     /translation="DDWIFGGRRSDVLVGGRGNDTVFGDNGNDRVNGGNGNDHLYGGD
                     GADHLYGDAGNDYLNGGKGGDWLYGGAGNDTFDYNALSDSPGFEETSPVIVRDSIVGF
                     NGDGAALGDRIDLSSIDANLDVDGNQAFTPAQLSYSNGIFSANVIGFGIPGSLDLQIH
                     LVGNPPLDIVGATNDIIL"
BASE COUNT         1016 a         1150 c         1180 g         1030 t
ORIGIN      
        1 gatcgaggct atgccagagt cgggccacgc atctgtttcg cggtttaccg acctgctgcg
       61 atgttttgca gcattgacga catattacaa gtaaatcttg taattgtcaa gaaacttgta
      121 aaggggggat gtgaaaaaga acgcaagaaa cacttgcaaa accgaatggc tgaaatccga
      181 gcagggaatg acgggaatag ggaatgcatg gggtagcgcc ccgaagcgtg ggcgagggaa
      241 cgcacgttgc agcgcagaca tagcgagccc gtggcccagt tgtgcaacgt gcaccgcgct
      301 ttttcttttt cgtacgcaac ccagagggac gagatgaaat ttcatccgac ccatcgttac
      361 tcgcggctta gaggctactc cgccgcgccg ctcatgcctc gttccagttg aaattcgtca
      421 ccgtcgcggc cgaaaaacaa gtattaacag gccctggtcc ggcctgctcc tatcttgcag
      481 gtacccgaac gaagggggcg gagttggatt cccgctggtg cgctctcccc gggccatact
      541 caaccacgtg gtcgtccttg aggatgactg tataatcgac aagatcctgg atagcacctg
      601 aaaaataaga ccaggcactt gtccgcctct caagatagag caaaacttcg taatcaccgc
      661 tacgctgaaa tccgtccgga tcgcccgcaa ccttgatcac ttcagctttt gtcatgcctt
      721 cgcgcagtcc tgtcattttg ctgcttgcgc acgcggaaag cgccagtgcg aagagcaggg
      781 cgagcacggc cgatttcata agttgtctcc caaagaaaat atgtttattt tgttttttcg
      841 gtgcgtcaga gtgtagctag ctctccagct ccttgatgag catcgccacc tccgcaattt
      901 ccgcaagaac ttctttcacc gcgagttcaa ggggatacct gcgtttttca gtttcgcgca
      961 tgtgttcggg aaacttcatg agatgctgct cgcgaattgc gcgcgctttc tcggacacat
     1021 ggaaggcaaa caacatttcc gctgtgtcat agggtgttga gttcatcact gcctccgtcc
     1081 tgccctagca atcccctgga cttcactctc gctcgccaac aggttttctt tttttcacaa
     1141 ccattcatca gcaagcctcc tctttgttcc agcacgattc gcgttgttcc agcatagtgc
     1201 ttcttcaatt ttagctcaat cggtatattt gggcctttat ttgagggatg gggaaacgtc
     1261 gtttcccgct atcatttatt gtccgaaggt agcgacatcg ggtaaaggcg gcttaggttc
     1321 cttaggtgct tagcccctgc tacgcctgtc gcatttacgg cggtccggtt ggtgccgggg
     1381 aaacagtcgc ctatctgccg attgccgtct ttccccttgc ctttgttctg ttccccccgg
     1441 gcagcctacg cggtggaaat gccggtggcg gatgaggagc caggtctggg gctggcaaag
     1501 gcttcaggtc cactgctgcc tcttcaattc gcgcccctcc agcgagtgcc ttgcttatcg
     1561 tcacaagacg cgccgcatct tccactgaca ttcgacgcat ggcctccacg acctcgtcaa
     1621 tgagcggatc gcttgtcgat ggcggctttt caggagcact tgatcgggcc gctctcctgt
     1681 ctttcttata ttccgcagct ttcggttctt gcaccgcttt ggcaggcgta ctttccgctg
     1741 cttcggtaaa tatgccgaaa tgcataagcg tcggagacag cttcaatact tccgcgattt
     1801 tgctcagcct tttcccccgc ggctgagtcg ccccggactc ccactgctga acagcctggg
     1861 gggacacccc caccaggtcg gccagttgag actggttcag gtttagtttt tcgcgcgcct
     1921 cgcgaatgac cttcgcaaaa ttcatacact catgatacaa gtaaatcttg ttgactgctt
     1981 acaagaatta cttgacatgt acaagtatta cttgtaataa tggtgacttg ttggaggctt
     2041 gaacctctga aaagtttcta ctcatcatca aacgcgaaat ggctggcgtg atatccgatt
     2101 ttcgagggga atggctcgcg aatgaattat tacgaacgat attgtggaga ttatcagcgc
     2161 gacatggtgc atctctcgat ggccaagcat agggcgtagg cgatgctgct ggaacctatt
     2221 tcagcgtcga aaagccgttg cccagtgatt tggcgagcct ttacagggtt tgcagggcca
     2281 tgacctcggt ggagcagcag gccgtgaagg cagttgccga acaaacaatg gcaggcgaga
     2341 ttcccatgcg aattaatgat tttcaggaac agcaaatgat gcaagcagta cggacatgtg
     2401 gttatgacaa aaaggggcaa ttggacttat gaaaaatctg agtgagggca aaggaggcag
     2461 ggtggtgaca agattccggt caggaattgc agttgcatat atgggagcaa tcgttggggt
     2521 cgggaatgga gaagttcagg cgggtgggcc cgcctccacg ggtactgtag caggcacggg
     2581 aacctcgtct gcggcaagcg cttttgtttc atcgcagcag gcccagatgc agagccagat
     2641 acagattcag aataactcgc agatgcagac atcgtcgtca ggagcaacgg gaggggaagg
     2701 gggatcggcg aacaacgctg gaaacagcca agtcgtaaat ctcgccaact caggtgtgcg
     2761 aaacaatcct gccattggac ttggccttat ggttcctact gcaccctgcc gcgacaccta
     2821 ctctataggt gcgtcagccg ctggcttggg cggtgcgctc gcatggtcga aagccgatcg
     2881 cgaatgccgc ttgcaggata ttggtgctgc ctttatccgc gcagggttgg tcgatgacgg
     2941 tcttgccttg tggtgttcga tcgacggggt cgatgaaatc gcgcccacct gccggcgttt
     3001 gaagacggca agccggaaag cacccgcagt tgccggggcg cccggcatgg atcaagagaa
     3061 tgcaagtgac gaagcagtaa acgaatttag ctggtgattc aaaaatcaat ttatagatcg
     3121 caagaaagtt caatccaaac caactgcata gaaggaaaag tgacaatgaa attcagaaat
     3181 ccggaacacg cgctgagatg ggcttatgaa acgagcaata gccccatcgt taaaatctcg
     3241 tcgatccatg caatgcgtgg ggccgacagg ggagagggag ggctttcaaa agacgagttg
     3301 acgccctacg acttccatgc tcaggcggca ctgatactga gcttatgttc gagcgtccta
     3361 tcacccttgc atatggccta cgtgagtatc cagttcggaa gggaagcggc cggattcaat
     3421 ctgctcggac gtcagctcgc ggcgaatttt ggcaccggga tgcatagcag gaaggggatc
     3481 gagcacgtta ttcgcgcata ctgcggagaa aaaatcgggc tgcgggagct acgtaaaacg
     3541 atgtcctgcg gcatgctcaa ggcggcatca tttcgcaacc ggggttacga catgctggat
     3601 gcgatccatg cccaggcgct gaatatcctg cgaagggaaa tggaaagcaa gggattggtg
     3661 cggtgcgagg acgccaggga gatagcgtag gatttgttga aaagcgcgcc tgcttattgc
     3721 ggaattcctc cgctgacgcg cactctgtta atcaacattg agcaaagccg gggctttatc
     3781 gattgttttg ccaatatccc cggctttgca ggtgatcaag acgtcttatg cgaccgcacc
     3841 tataagataa tgtcgttcgt cgctccgaca atgtccaaag gcgggttgcc aaccagatgg
     3901 atctgcagat cgagcgatcc tggaatacca aacccgatca cattcgctga aaatattccg
     3961 ttgctatatg acaattgggc tggagtgaag gcttgatttc cgtcaacatc gagatttgca
     4021 tcaatactcg agaggtcgat ccgatcccct aaggcagccc catcgccatt gaaaccaaca
     4081 atactgtcgc gaacgataac cgggctggtt tcttcaaacc ccggactgtc agacagcgcg
     4141 ttgtaatcga atgtgtcatt gccagcgccg ccataaagcc agtcccctcc cttgccacca
     4201 ttcaaatagt cgttaccggc gtccccgtaa agatgatccg ctccgtcgcc gccatacagg
     4261 tgatcgttac cattgccccc gttcacccgg tcgttaccgt tatcgccaaa gacggtatca
     4321 ttccctctcc cgcctaccag tacgtctgag cgacggcctc caaaaatcca gtcgtc
//
LOCUS       DASSHJ010000260        10736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1376597,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000260 DASSHJ010000000
VERSION     DASSHJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376597"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..611)
                     /locus_tag="VFI43_10060"
     CDS             complement(174..611)
                     /locus_tag="VFI43_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HET7062508.1"
                     /translation="MTTDKGQANEPDKKHTSQPGNGREQQNVPRLSGVILDEEHFLTL
                     TEISQACAVQTGYIIELVDEGLLTPETSARSRTSEPEPHFWRFTGEQMCRARTAARLQ
                     GDLGINLAGVALVMQLLDEIEELRERLEAMQLAQQPTGRMGGA"
     gene            complement(608..1591)
                     /locus_tag="VFI43_10065"
     CDS             complement(608..1591)
                     /locus_tag="VFI43_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET7062509.1"
                     /translation="MEYKDYYKIMGVARDASQDEIKRAYRKLARKYHPDVSKEPQAET
                     RFKELGEAYEVLKDPEKRVAYDQLGSNWKANQEFRPPPDWNAGFEFSERGFTGGNASQ
                     YSDFFESLFGGAFRAGQGARGPGTAGATSGYGYHAPGEDHYAKVMIDLEDSYNGATRT
                     IALQVPEVDPQGHVSTREHTLNVVIPKGIKPKQNIRLAGQGAPGIGQGKRGDLYLEIE
                     FRPHPYYRVEGADVYLELPVAPWEAVLGATVKVPTPGGTVELKVPAGSTTGQKLRLKG
                     RGIPSKTPGDFYVVLRTEPPPATGEADKAFYLKMAEQFKSYNPRAKLGVEA"
     gene            complement(1874..2398)
                     /locus_tag="VFI43_10070"
     CDS             complement(1874..2398)
                     /locus_tag="VFI43_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7062510.1"
                     /translation="MSLTIPVNEHDHTQGPSDARVTLVEYADYQCPYCSKAYVIVKQL
                     EDQFGPLLRFVFRNFPLTQIHDQAELAAEAAEAAGAEGKFWQMHDALYENQPLLDADL
                     IRTVAQELGLDMKTFENDVETGKFRERIEHDFMGGVRSGVSGTPTFFINGSLHQGKWD
                     QAALSAAIGVELDK"
     gene            complement(2683..3258)
                     /locus_tag="VFI43_10075"
     CDS             complement(2683..3258)
                     /locus_tag="VFI43_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET7062511.1"
                     /translation="MNDMISKSWKVLALRGVISILFGVLAALWPGLTLMWLVVLFAAW
                     ALVSGFGYMSAAVNNRKTHSDWWMLLLMGLIGLGAGIIAILNPGLTTVFLVLLIGAMA
                     LANGVVEIAMAIRLRKSIQGEGLMIVSGIVSVLFGAFLFFFPGAGALTMVWLISIYAI
                     VTGVLLLLLAWRAKKWTGHEENFPPAVPHGT"
     gene            3628..3999
                     /locus_tag="VFI43_10080"
     CDS             3628..3999
                     /locus_tag="VFI43_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062512.1"
                     /translation="MKIGDGKNGEGESREEESKRSGKPIPFEKETGLHRQRVDLPDPD
                     PDAKNQTEEQPLLPNERDETTRPTATSLGTENEMSREVVEQAREDTEHGLRDTDLRGI
                     PSDIIAADVPPADENKKQGDG"
     gene            4117..4572
                     /locus_tag="VFI43_10085"
     CDS             4117..4572
                     /locus_tag="VFI43_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET7062513.1"
                     /translation="MKASFKVIAGLAVVLVSSFPTLSSGQDSVQDGASSLPPVQSQGQ
                     TEFLTGGVGKDESDAIQQVAKSWPLTLELAEASPPRPQYISDVQIRIKNKSGNMVLDA
                     NAEGPFVLVKLPPGKYGIDATYESQTIHRDVTIQDGQNRKLTLMWGIPR"
     gene            complement(4544..5557)
                     /locus_tag="VFI43_10090"
     CDS             complement(4544..5557)
                     /locus_tag="VFI43_10090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7062514.1"
                     /translation="MKSVGKPASEPLDCLIVGAGPAGLTASIYLARFRRKIAIIDSGF
                     SRASLIPASHNCPGFPDGVSGKELLRLLRLQAAHYGVEVEAGEVSNIKAVKGGFEATV
                     EGCAEQEHPPDKNPFIVHAATVVLATGTTDNIRNIPDWIIGVERGLLRFCPICDAYEA
                     ADEHIAVLASSAARGVNHALFLRTYTRKLTLILSQAVLTAAEQTKLRRAGIDVIEDTP
                     REIRTAGEKKPAVVLSDGKVLCFDVVYPMLGESARSGLARQLGARCNRHGKLVVDRHQ
                     RTTVPAVYATGDVVDQLNQISVAMGHAAIAATDIHNRLGSLSRWLRKRTDQGFIGESP
                     TST"
     gene            complement(5706..6431)
                     /locus_tag="VFI43_10095"
     CDS             complement(5706..6431)
                     /locus_tag="VFI43_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062515.1"
                     /translation="MTRPGIRPSLIIKTLILIILPVALTAGCSTAHKPTQSQRSAIEQ
                     LLMSEAIMRSLPAGSTPSLPIPEGASVVLDASVINMSTVPSPDKFLLEKVLAGWFGKQ
                     GYAIQKNEDKASYRVNIVTTTLGTELGDSFFGMPPVTSVLIPFSLPELAVYKNQYQTG
                     YVKFYMDIFELPAGKLVHSSPFYVAETYYNDYTILLFFSFRSTDLPLAPELESFHKPM
                     EKRIREIEEEKSPRNSWWELENY"
     gene            6616..8319
                     /locus_tag="VFI43_10100"
     CDS             6616..8319
                     /locus_tag="VFI43_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HET7062516.1"
                     /translation="MKPNNLKYSFPALKLIDFAQLQFENRFVGHLPADPVVHNIPRAV
                     RNACYTRVHPTPVSAPRLLAWADPVGEMLGIARPELSSGQATQVLGGNRLLRGMQPYA
                     ARYGGHQFGHWAGQLGDGRAITLGEVSAPDGGRYELQLKGAGKTPYSRTADGRAVLRS
                     SVREFLCSEAMHYLGVPTTRALSLVTTGEQVVRDMFYDGNPQPETGAIVCRVSPSFVR
                     FGNFEILRVQNELDVLKRLADYVIGEHFPELTAPLPATGSPLGTGVYAAWFEEICRRT
                     AILVAHWMRVGFVHGVMNTDNMSILGMTIDYGPYGWLEGFDFSWTPNTTDAQGRRYCF
                     GNQPDIAYWNLMRLAAALAPLTEDNEALEQGLKTFGETFNKSWLGMLASKLGFSAFHH
                     AKDDALVNDLFDLLQKVETDMTLFFRRLMRLPMNDVAGVNIDKSNGNNSISPGDRELV
                     ELFRPAFYDERQAFTPEAMSHLVGWLRRYAERLRMEKEPEASRHQRMSHANPKYVLRN
                     YLAQQAIEALDQGDDSVMLRLMEVLRRPYDEQPGHEDLAARRPEWARDKPGCSALSCS
                     S"
     gene            complement(8352..8591)
                     /locus_tag="VFI43_10105"
     CDS             complement(8352..8591)
                     /locus_tag="VFI43_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062517.1"
                     /translation="MTWKTVERYSLGYDIQLKQFYVYYELEGDKAAHQISTSPPEFLS
                     LSDMFRNEGPISFNVEGQYFVTRAIPVGDQGDGLL"
     gene            8826..10361
                     /gene="pyk"
                     /locus_tag="VFI43_10110"
     CDS             8826..10361
                     /gene="pyk"
                     /locus_tag="VFI43_10110"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7062518.1"
                     /translation="MGLSKSQLPWHRTKIVCTLGPSTDAPNVVERLIANGMDVARVNT
                     SHGNHAEHAQRIRRVRQAAQEAGQPVAILLDLPGPKFRIGNVPNGSLKLVEKTTVALG
                     ANDELEGDSSSKDWSYLPIRNPELIQAVVPGERIFLADGLIELLVQAVVSGSVECKVL
                     IGGVVRSGSGINLPESTLDALVPTEEDRRHLAFAVAQEAEWVGVSFVQTAADIMRVRA
                     LLPDGARTLLMAKVEKRKALQNLDQIVEAADGVMVARGDLGIETDLAQIPIVQKRIIA
                     VANAKARPVITATQMLESMVEHEHPTRAEVTDVANAVLDGTDAVMLSAETAIGRFPAL
                     SAGMLDRILAATEASGIAQGMGEIDSRWVAPWAAGSAPVGAAPSEPGNRLCNVRSNAD
                     SYTWAHTLSGAACRLGAGLGAKAIIAQVCTVAEAMNIARFRPSIPTVMTTESLHLYRS
                     LALVRGIAPQLAPASSDERVTAIARAREWLFAHQLAKPGDAAVLLYASANGEADSLQV
                     IWL"
BASE COUNT         2311 a         2964 c         3073 g         2388 t
ORIGIN      
        1 gagacgtctt agaagaggct cgcggccgag ctatagatgc tgatgcgtac gaggagcctc
       61 gaagcctaag tttgcctgat gctatttgca ggaatcctcc tcaccctcgt gcagctgttg
      121 caagctagag caagtcgagc gcttccttct aatatatcat gttgatgggt tcgctatgct
      181 ccacccattc tgcctgtagg ttgctgcgcc aattgcatcg cctctagccg ttcccgaagc
      241 tcttcgattt catccagcag ctgcattacc agtgccacgc ccgcaaggtt aatgcctaga
      301 tcgccttgca gtcgtgcagc cgttctcgcc cggcacatct gttcaccggt gaatcgccag
      361 aagtgaggtt caggctcaga ggttctcgat cgagcgctgg tttcgggagt aagcaggcct
      421 tcatcgacga gttcaatgat atatcctgtc tggacggcgc aggcctggct gatttccgtc
      481 agggtcaaga agtgctcttc atcaaggatg acgccgctca gcctggggac attctgctgt
      541 tcccgtccgt ttcccggctg gctcgtgtgt tttttgtccg gttcgtttgc ctgcccttta
      601 tccgtggtca tgcttcgact cccagtttcg cgcggggatt ataggatttg aattgctcgg
      661 ccatttttag gtaaaacgcc ttgtctgctt ctcccgtggc aggcggtggt tccgttcgca
      721 acacaacgta gaagtcgccc ggcgtttttg acggaatccc tcgacccttc aatcgcagct
      781 tctgcccggt tgttgaaccc gccggtacct tgagctcgac ggttccgcca ggggtaggca
      841 cctttactgt tgctcccagg accgcttccc acggagcaac gggcaactcc aggtatacat
      901 ccgcgccttc tacacgatag taaggatgag gccgaaattc aatctcgagg tacaggtcgc
      961 cccgttttcc ttgcccaatg ccgggagcgc cttgcccggc aagccggata ttctgcttgg
     1021 gcttgatccc cttggggatc acgacattaa gcgtgtgttc ccgagtggat acatgtccct
     1081 gcggatcgac ttcaggcact tgcagcgcga tcgtacgggt agcgccattg tacgaatcct
     1141 ccaggtcgat cattaccttc gcgtagtggt cctcacctgg ggcgtgataa ccatatccgc
     1201 tcgttgctcc tgccgtccct gggcctctcg cgccttgacc ggcacggaag gctcccccga
     1261 acagcgattc gaagaaatcg ctgtattggg aagcgttacc cccagtaaac ccgcgttccg
     1321 aaaattcgaa gcccgcgttc cagtcgggcg gcggccgaaa ctcctggttc gccttccagt
     1381 tcgatccgag ctgatcgtac gcgacgcgtt tttcggggtc cttgagcact tcgtatgctt
     1441 cgccgagttc cttgaagcgc gtctcagcct ggggttcctt gctgacgtcg ggatgatatt
     1501 ttcgcgcgag tttcctgtag gcgcgcttga tctcatcctg ggaggcatcc cgggcgacgc
     1561 ccatgatttt gtagtaatcc ttgtattcca tctattcgtg acgatgaaca aatcaatgct
     1621 tcagtcttca tatcaagact agcataaccg gacgcttttc cggcaatctg aaacggcgag
     1681 cttttgctat gactcagctt ttgctttaac ccatgagccc gattccattg cagagccagt
     1741 gactcatcgc aatgttatgc gaaatgaagg ctaccggggc aatgatttat ttgggttcgg
     1801 gcctttatgc tgcacagccg cattatgacg aattcgtctc ttcgttccat accggaaaac
     1861 cgtggcaccg cattcattta tccagttcca ctccgatggc cgcgctgagt gcagcctgat
     1921 cccattttcc ctggtggaga ctgccattga tgaaaaacgt gggtgttccc gatacgccgc
     1981 tacgcacccc gcccatgaaa tcatgctcga tgcgttcacg gaattttccg gtttccacgt
     2041 cattttcgaa ggttttcatg tcaagcccaa gctcctgggc tacggtccgg atcaagtcag
     2101 catcaagcag cggctggttt tcgtacaatg catcatgcat ctgccagaat ttgccttctg
     2161 caccggcagc ttcggcggct tcggccgcaa gctctgcctg atcgtgaatc tgggtcaagg
     2221 gaaaatttcg aaatacgaaa cgcagaagag gcccgaactg atcctcaagc tgtttgacga
     2281 ttacgtacgc ttttgaacaa tagggacact gataatcagc gtattcgacc agcgtgacgc
     2341 gggcgtcgga cggcccctgt gtgtgatcat gctcgttcac aggaatggtc aggctcatgt
     2401 gtgccctccg gaaattgatt ccagggcgcg taaaacgcca tctgtccccg ggttgacgcc
     2461 aatcggcgaa acataactgg gctgctgatg cattgcaggt ttttgcaaat ctttgatgtc
     2521 aagagagcag gggttttctt cggaaccttc ccagtggggc ttggggcaaa cgtgctggcc
     2581 ggaatttcac ccctgctcgt atttttggaa gctttatttc aggtttctaa ggttgttcaa
     2641 gcacagcgtg gtttggctga actccattat ttcccctatt cctcaagtcc cgtgtggaac
     2701 agccgggggg aagttttctt cgtggccagt ccattttttc gcccgccagg ctagcaaaag
     2761 cagcagcacg cctgtgacga tcgcgtaaat gctgatcagc cataccatgg taagcgctcc
     2821 cgcacccggg aaaaagaaga gaaacgcccc gaacagcacg gaaacgattc cgcttacaat
     2881 catcaatcct tctccctgga tagacttgcg caagcgtatt gccatcgcaa tctcgacaac
     2941 tccgttggcg agcgccatgg cgccgatcag aagtaccagg aatactgttg tcaacccggg
     3001 gttgaggatc gcgatgatcc cggccccaag gccgatcagc cccatcagga gcagcatcca
     3061 ccagtcggaa tgcgttttac gattgttgac ggcagcactc atgtagccaa aaccgctgac
     3121 gagcgcccag gcggcgaaca gtacaacaag ccacatcagc gtgaggccgg gccacagcgc
     3181 cgcaagcaca ccgaacaata tcgagatcac gccgcgtagg gcgagtacct tccaggattt
     3241 agaaatcatg tcattcatga tgtcacctct cattcgagaa cagcgcatcg gtcgcatccg
     3301 tcaattgcaa ttgcagtgca atccgctacg cccataagtc gtatcgtgca ccctccgatc
     3361 cgtttatgca gtgcggtaac agacatacga atcgaggggg ataaacaatg aagccaaata
     3421 ggaattcgag gcgaaaggac tgaaaggact gatgcgaggc cgtgcctgag tcaagggggg
     3481 agagttattt gttggagaga cgatgggttc tcccgaactg ctatgtggga cggcgcacgg
     3541 tcattttccg cctgattccg ttaaagtaaa aaatttcggc tagaagcgcc gcacttgaag
     3601 catggatcga atcaaaggat gagagagatg aaaattggtg atggcaagaa tggtgagggg
     3661 gaaagcaggg aggaggaaag caagcgaagc ggcaagccca ttccttttga aaaagagaca
     3721 gggctgcatc ggcaacgggt cgatcttccc gacccggatc cggacgcgaa gaatcagacc
     3781 gaggagcaac ccttgctgcc aaacgagcgt gacgagacta cccggcccac tgccaccagc
     3841 ctcggtaccg agaacgagat gtcgcgcgaa gtggtcgagc aggcgcggga ggataccgag
     3901 cacgggctca gggacacgga cctgcggggc attccatcgg acatcatcgc tgcggatgtg
     3961 ccgcccgcgg atgagaacaa aaagcagggc gatgggtgat ggtggggttg agcgcgagtc
     4021 gagatcaaga atggtgaagg ttattttgtt ggattcccgt tttcgaaggg aacgacatcg
     4081 ggtgagggta ggcgtttgca aacaggaggt actggaatga aagcatcctt taaagtgatc
     4141 gctgggttgg cggtcgtcct ggtgagttct tttcccactt tgagttctgg gcaggattcg
     4201 gtccaggatg gggcgagcag cctgccgccg gtgcagtcgc agggccagac ggaatttctt
     4261 acggggggag tagggaagga tgagtcggat gcaatccagc aggtagcgaa gtcgtggccg
     4321 ctgacactgg agcttgctga agcatcgccc cctcggcccc aatatatcag cgacgttcag
     4381 atcaggatca agaataagtc cggcaacatg gtgctcgatg caaatgccga aggacccttc
     4441 gttctggtca agctaccccc tggaaaatat ggcattgacg ccacctatga atcgcaaacg
     4501 attcatcgtg acgtcactat tcaggatgga caaaacagaa agcttacgtt gatgtggggg
     4561 attccccgat aaatccctgg tctgtacgtt tacggagcca gcggcttagc gatcccagcc
     4621 ggttgtgaat gtcggttgcg gcaatggctg cgtgacccat ggccacactg atctggttca
     4681 gttgatcaac gacatccccg gtcgcataca cggcagggac agtcgtgcgc tggtgccggt
     4741 cgacgacgag cttcccgtgc ctgttgcaac gagctcccag ttgcctagcc aagcctgatc
     4801 ttgcactctc cccgagcatg ggatagacaa cgtcaaagca tagcactttc ccgtccgata
     4861 gcaccaccgc gggctttttc tctcccgcag tgcgaatctc tcggggcgta tcctcgatca
     4921 catcgattcc cgcccggcgc agttttgttt gttcagccgc tgtcagcacg gcttgggaca
     4981 ggatcaaagt cagtttcctg gtataggttc gtaaaaagag agcgtgattc acgcccctcg
     5041 cagccgagga cgccagcacg gcgatatgct cgtcagcagc ttcataggca tcgcaaatgg
     5101 gacaaaatcg caaaaggccg cgctcaacgc cgatgatcca gtcagggata ttgcgtatat
     5161 tgtcggtggt gccggtggca agcaccacgg tggctgcatg aacgatgaac gggttcttgt
     5221 cgggtgggtg ctcctgctca gcacagcctt ccaccgttgc ctcaaatccc cccttcacgg
     5281 ccttgatgtt ggaaacctca cctgcttcaa cctcaactcc gtagtgcgcc gcctggaggc
     5341 gcaggagacg cagcaattcc ttccccgaga cgccatcagg aaaaccgggg caattgtggc
     5401 tggcagggat gagcgaagca cggctgaacc cgctgtcaat aatcgcgatc ttgcgccgaa
     5461 accgcgccag gtagatcgat gcggtcagcc ctgcaggccc cgccccaaca atcaggcaat
     5521 ccaagggctc ggacgctggt tttccaacgg atttcatggt tgaatctctt cggaacgtgg
     5581 aaagggatag cagctggaaa ccaccaccat tctggctgga atgcttccta cattccgtac
     5641 gggacaacac attcattagt cacggaaagg tccggggaag gtttgagtaa atcgccctga
     5701 catactcagt agttttccag ctcccaccat gaattccggg gcgatttttc ttcttctatt
     5761 tcacgaatcc gcttttccat cggcttgtga aaagactcaa gctcaggcgc cagggggagg
     5821 tcggtcgatc tgaaggagaa aaacaacagg atggtgtagt cgttgtaata ggtttccgcc
     5881 acgtagaatg gcgaggagtg tacgagcttt ccggcgggca gctcgaaaat atccatgtaa
     5941 aatttcacgt acccggtctg gtactggttc ttgtagacgg caagctccgg cagggaaaag
     6001 ggaatgagca cgctggtcac aggtggcatc ccgaaaaagc tgtcgcccag ctctgttccc
     6061 agcgtagtcg taacaatatt gacgcggtag gatgccttgt cctcgttttt ttgaatggca
     6121 tagccctgct ttccgaacca tccggcgagg actttttcca acaggaattt gtcgggactg
     6181 ggaacggttg acatgttgat tacggacgca tcgagcacaa cgcttgcgcc ttccgggatt
     6241 ggaaggctgg gggtggaacc cgcaggcaga ctgcgcatga tggcttcgct catgaggagc
     6301 tgttcgatag cagaccgttg agattgcgtt ggcttatgcg cggtcgaaca gccagcggtc
     6361 agggcgacgg gcagtatgat caggattagc gtttttataa tcaaactggg tcgaataccc
     6421 ggtcgcgtca tggaatcttt cctccagtta ctatgactgt gtattggtgg ttgcaacaaa
     6481 acggttcatt tgttgtccat ccgcgtgtga ttgatgttcc ctcgcttgtg gtcgacgcgt
     6541 tttttgctgc gggctgaccg cgaatcaccg ctggcctggc cagcgccccc catccaccac
     6601 ctcaggcaca cttaattgaa gcccaacaat ctcaagtata gcttccctgc actaaagctc
     6661 atcgattttg ctcaactgca attcgaaaac cgattcgtgg gtcatttgcc cgcagacccg
     6721 gtcgtgcaca acataccgcg ggctgtacgc aacgcctgct acacgcgcgt gcacccgact
     6781 cctgtgagcg caccacgcct tctcgcctgg gccgatccgg tgggcgaaat gctcgggatt
     6841 gcccggccgg aattgtcctc gggccaggct acgcaggttc tgggcggaaa ccggttgctt
     6901 cgcggcatgc agccctacgc cgcgcgttac gggggacacc agttcggtca ctgggcaggg
     6961 cagctcggcg acggccgcgc catcacgctg ggtgaggtga gcgcgccgga tgggggccgc
     7021 tacgagctac aactcaaggg ggcgggcaag acgccttatt cgcgcacagc cgacggccgg
     7081 gcagtgctac gctcgtcggt acgcgagttc ctttgcagcg aggccatgca ttatcttggc
     7141 gtcccgacta cgcgtgcatt aagcctcgtg accaccggag agcaggtggt gcgggatatg
     7201 ttctacgatg gcaacccgca gccggaaacc ggcgcgatcg tttgccgcgt ttcgccatca
     7261 ttcgtgcgct tcggcaactt cgaaatactc agagtgcaga atgagctgga tgtgctcaag
     7321 cgtctggccg attacgtgat aggggaacat tttccggagc tcacagcgcc attgccggca
     7381 accggttcgc ctctcggtac cggtgtttac gccgcctggt tcgaggaaat ctgccgccga
     7441 accgctatcc tggtcgcgca ctggatgcgt gtgggctttg tccacggcgt gatgaatacc
     7501 gacaatatgt cgatcctggg gatgaccatc gattatggac cctacggttg gctggaaggc
     7561 ttcgacttca gctggacgcc gaatacgacg gacgcgcagg gaaggcgcta ctgtttcggc
     7621 aatcagcccg acatcgcgta ctggaacctg atgcgcctag ccgccgcgct ggctccgctc
     7681 acagaggaca acgaggcgct tgagcaggga ctgaagacat ttggcgaaac cttcaacaaa
     7741 tcgtggctgg gcatgctggc aagcaaactc ggcttttccg ccttccatca cgccaaggac
     7801 gacgcgctgg tgaacgatct tttcgatctt ttgcagaagg ttgaaacgga tatgacgtta
     7861 ttcttccgcc gcctgatgcg ccttcccatg aatgatgtcg caggagtgaa cattgataag
     7921 agtaatggga ataattccat ctctcccgga gacagggagc ttgtcgagct gttccggccg
     7981 gcattttatg acgagcgcca ggcgtttacc ccggaagcca tgagccatct ggtgggttgg
     8041 ctgcggcgtt atgccgaacg tctacggatg gaaaaagaac ctgaagcctc gcggcatcaa
     8101 cggatgagcc acgccaaccc caagtatgtg ctgcgcaatt acctcgcaca gcaggcgatc
     8161 gaggcgctgg atcaggggga cgactcggtg atgttgcgtc ttatggaagt gctgcggcgg
     8221 ccctacgatg agcaacccgg gcacgaggac ctggccgcca ggcgacccga atgggcgcgc
     8281 gacaagccag ggtgttcggc cttgtcgtgc agttcatagg ccgggcgtcg aacagagtaa
     8341 accctggctt cctaaagcag gccgtcgccc tgatccccga cgggaatcgc gcgggtgaca
     8401 aagtactgcc cttccacgtt gaaactgatc ggtccctcat tcctgaacat atctgacagg
     8461 ctgagaaatt ccggcgggct ggtcgatatc tggtgagccg ccttgtcgcc ctccagctca
     8521 taatacacat agaattgctt gagttggatg tcgtacccaa ggctgtagcg ttccaccgtt
     8581 ttccaggtca ttcttaacct ccacagtgtt cgtcctgctg caactccgcg tgccgccaat
     8641 gccgaagcag gtcgtttcgg atttcctcag tcgatgttcg ctttttacgc gaaagaggcc
     8701 atgctcatta tttaaataga gcatatggta atcttaatca aggcgcgatc accttcaacc
     8761 gccgatcgcg catgaatcgc atcaacgatt ttctgattga cgaatgaatt gcagtgagcg
     8821 gacgcatggg attatcaaaa agccagttgc cttggcatcg gacaaagatc gtttgcacgc
     8881 tgggcccatc cacggacgcg ccgaatgtcg tcgagcgatt gatcgccaat gggatggatg
     8941 ttgcaagggt aaatacctcg cacggcaacc atgccgaaca tgcgcagcgc attcgccgcg
     9001 tcaggcaggc cgctcaggaa gcgggccagc ccgttgccat tctgcttgat ctgccgggcc
     9061 cgaagtttcg tattggcaat gtacctaacg gcagcctcaa gctcgtagag aaaacaacgg
     9121 tcgcgctggg agccaatgat gaacttgaag gcgatagcag cagcaaagat tggagttatc
     9181 ttcccatccg caacccggag ctgatccagg ccgttgtacc gggagaacgg atatttcttg
     9241 ctgatggctt gatcgagcta cttgttcagg ccgtcgtgtc cggcagcgtg gagtgcaagg
     9301 ttctcatcgg cggggtggtt agatcgggtt cggggatcaa tctcccggaa tcgacactgg
     9361 acgcgctcgt tcctacggaa gaggacaggc ggcatctcgc cttcgcggtg gcgcaggaag
     9421 ccgaatgggt aggggtgtcg ttcgttcaga ccgcagccga catcatgcgc gttcgcgcgc
     9481 tgcttccaga cggtgcgcga acgctgctca tggccaaggt cgagaagcga aaagcgcttc
     9541 aaaacctcga tcagatcgtg gaagcggccg acggggtgat ggtcgcgcgt ggcgacctgg
     9601 gcatcgaaac ggatctcgcc cagattccga ttgtccagaa acgcatcatt gccgttgcca
     9661 acgcgaaggc caggccagta atcacagcca cccagatgct cgaatcgatg gtcgagcacg
     9721 agcatcccac tcgcgccgaa gtaaccgacg tcgccaatgc cgtgctggat ggaaccgatg
     9781 ctgtcatgct gtcggccgag actgctattg gacgatttcc cgcgctttcg gcgggtatgc
     9841 tcgaccgcat actggcggcg accgaagcgt cgggcatcgc tcaagggatg ggcgaaattg
     9901 attcgcgttg ggtagcgcct tgggcagcgg gcagtgcacc ggttggtgcc gcccctagtg
     9961 agcctggcaa tcgactgtgt aacgtccgca gcaatgcgga cagttatacg tgggcacaca
    10021 ccctgagtgg tgcggcctgc cggctcggag cgggtcttgg cgccaaggcg attattgctc
    10081 aggtttgcac cgtagccgag gcaatgaata tcgcacgctt tcgtccctcg atccccactg
    10141 tcatgacaac agaatcactg cacctgtatc gcagcctcgc cctggtgcgc gggatcgccc
    10201 cacaactcgc tcctgccagt tccgacgagc gcgtgacggc gattgcacgg gctcgagagt
    10261 ggttgtttgc gcaccagctg gcgaagccag gcgacgcggc ggtgcttttg tatgcgtctg
    10321 cgaacggaga agccgactcg ttgcaggtga tttggttata gcccgcgcct ggggttcctt
    10381 ctctccctgg ccgctctcca tcaagggaga gattcgaaaa atgatgtccg acgtcattta
    10441 tccctgttcc agtcactatg gatgcttcag catgatggat gctcagcatg ggaagtcgaa
    10501 ccgatggaag tcaactgatg ggtacggaga gtgcttcgct aaccatcctg aaaattccat
    10561 aaacagctta tggcaggatc ggtgaaaagg agtgaaagcg atggcctgcc ttgatcctgg
    10621 caggatggag cgaacgaggt ggaaccatta ccgtgccaac ccatcgtgct caacaattgg
    10681 tatccttacg gaatatctcc acaatctatg gaaccactgg aactcctgcc atgaat
//
LOCUS       DASSHJ010000261         7392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1143957,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000261 DASSHJ010000000
VERSION     DASSHJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143957"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFI43_10115"
     CDS             <1..328
                     /locus_tag="VFI43_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062519.1"
                     /translation="TSRDAAQNPAIQKAVLDAKTRNDHNALARYFENAASEMKTKAVE
                     QKKRLEHYEEKGYLYGRQAQDLKSHTAAMVRKYEDSAEENIREATLHRQKAAELMKGD
                     VALEGQ"
     gene            complement(612..1313)
                     /locus_tag="VFI43_10120"
     CDS             complement(612..1313)
                     /locus_tag="VFI43_10120"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HET7062520.1"
                     /translation="MKKKLVFVMLLIFGNPISAETQQLYGFRPQISLTKVVSDRWDFN
                     VFSSNNSNLSNNEFPGDIQHYFQASPIYKYSPNLNFVFLGYIYQRTNPFSDSYVNEQR
                     PFQQIVYGADSIFGRLTHRVRFEERFIYNRVTGQTPFSTRLRYQVALLVPLQGSELDN
                     GEFYFNIYNEFYFSLTGARNALYSENWSYAGIGYQTAEYGRIEIGPLIQRSIINQRHD
                     VRYINLLQVNWSYNF"
     gene            complement(1807..2349)
                     /locus_tag="VFI43_10125"
     CDS             complement(1807..2349)
                     /locus_tag="VFI43_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062521.1"
                     /translation="MMSRISIVLAASSVFFFAACASILPETPHSTEEEEVIRKKELPH
                     PVVMALPKGYEEREFTYKRKLKEGLVSYDVYYEKGGNQFAITYDPQGKILEQERRIKL
                     SDIPPATRKKIEKVLAARYPGYKTLMVEELYRNNEVLLEIFFSHPESKTGLVEAVFEL
                     HTGALREFINIKMKSIPTFN"
     gene            complement(2465..3181)
                     /locus_tag="VFI43_10130"
     CDS             complement(2465..3181)
                     /locus_tag="VFI43_10130"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HET7062522.1"
                     /translation="MKIFVFIMFMIVGNRLYAGDTHEFYEFRTQISLTKQVFDRWDLN
                     IFTSENANLVDKTYGGEQPPTNVQNYFLVGPTYKYSPNLNFVFLGYIYQRTSPVFDNF
                     FNENRIFQQVVYGTDFGFGRVTHRVRFEQRFIHDKAPEQKFLGTRLRYQLGLLVPLQG
                     EELNKGEFYFNIYNEFYFITSGPSGATYNENWAYAGIGYQTAKYGRIEIGPLLQRVVV
                     DKHDIRNFNILQFSWSHNFD"
     gene            complement(3427..4089)
                     /locus_tag="VFI43_10135"
     CDS             complement(3427..4089)
                     /locus_tag="VFI43_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062523.1"
                     /translation="MEAIYNAVLAFMLTSFLFSAPAAYAVNEQHTQQPLTGLQGKAVR
                     YLINPFGEVDGLFLDNGTLVKLPPHMSRDVAELVKPGDIVALQGAPEGGSSFEAYSIT
                     NIESNQTLLSRKPLWNGKVMPKELRAAELKELSAKGKIQRIISGKHGEPRIVVLENGT
                     NVRLPKDTAYDAYLLVHTGAPFAAKGRGTETRYGRSLEAFAVGISLASLKPLLNASQR
                     HH"
     gene            complement(4578..5117)
                     /locus_tag="VFI43_10140"
     CDS             complement(4578..5117)
                     /locus_tag="VFI43_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062524.1"
                     /translation="MSPIGRVLVASSLVFLNGCVATVPATYHKTEVEETIRVKDLPLR
                     VLAALPKEYDKKEFTYKRKHKNGKISYDVDYEKGGKKFSIGYDEQGRILEEEKKVEFS
                     DIPQDLRAKVEKVLSEHYPDHKILLVEEVYTPDKMLLEVFFSAPKSSTGFVEAVFEFK
                     TGVFREFVDIRMKSIQTLY"
     gene            complement(5561..6238)
                     /locus_tag="VFI43_10145"
     CDS             complement(5561..6238)
                     /locus_tag="VFI43_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062525.1"
                     /translation="MRTCHLLPVLLMSAISITPVAQAKKGHVRADEFLRVQAFATAAP
                     AAQGKVTRFLVNPFGEVDGLLLDTGNLVTFPKHMGEQLAAAVKPGDTVEIKGYPEAAG
                     QIKGYVITNSRSGESLMVQPKPPKGVMKMPKHLQSVSLRAMNAQGEIRHLHYGKRGEI
                     NGVILSDGTTVRFPHEASYQFAPMLKIGQSITVSGYGTKNQYGQALEATELAPAGEQP
                     QPVYQKR"
     gene            complement(6404..>7392)
                     /locus_tag="VFI43_10150"
     CDS             complement(6404..>7392)
                     /locus_tag="VFI43_10150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7062526.1"
                     /translation="NAALASSSRAIRQNPVPGEGEFVARSGRMRETVKLIGRAAASDA
                     TVLITGETGSGKEIIARALHQYSARASYPFVAINCAAIPHELLESELFGHVRGAFTGA
                     TASRVGIFAQAEGGTLLLDEIGDMSGNLQAKLLRVLAESVIVPLGAERPLSVNVRLLA
                     ATHRDLGQQVAEGRFREDLFYRLNVINIHVSPLRERREDIIALAEHFLALASDTPKAL
                     SAEARQCLETHTWPGNVRELRNVIERAMILARGASIDVADLGLVRTPAPLALPAGAPL
                     MHDLPTALAQLEEMMIRAAIAETDGNRTEAARRLGIRRQLLYAKLRQYGIEP"
BASE COUNT         1837 a         1855 c         1837 g         1863 t
ORIGIN      
        1 gacttcccgt gatgctgctc aaaacccagc catacagaag gccgtcctgg atgcgaagac
       61 acgcaacgat cataatgcct tggcgaggta ttttgaaaat gcagcaagtg agatgaaaac
      121 aaaagcagtg gagcaaaaaa agcggcttga gcattatgag gaaaaaggtt atctctacgg
      181 tcggcaggcc caggatttga agtcccatac agccgccatg gtgcgtaagt atgaggactc
      241 ggccgaggag aacatacgcg aggctaccct gcaccggcaa aaggcagccg aactcatgaa
      301 gggcgacgtt gcccttgagg gtcagtaaga aaaacggcgc gtcggcgccg atccgagcct
      361 gactcaacca accaggcgtg ccgatataat tacgttcagg ctaacactcc tatgatgagc
      421 gttagcctga aacaggcttt gcaaattctt caagatttca acatccggct tggatagaaa
      481 gccaagtcag cgttgttcag ttctcccccc ggattgtctg atcttcaggg caggcagggc
      541 ggccaactac tagcgtccat ctacggacct tcgatacagt gagggaattt gaggccttga
      601 gttctagtac gtcaaaaatt ataagaccag tttacctgca acagattgat gtagcgaaca
      661 tcgtgcctct ggttgatgat agatctctgg attagaggac caatctcgat ccttccatac
      721 tccgcggttt gataaccgat tcccgcatag ctccagttct cgctgtaaag cgcgtttcgg
      781 gcgcccgtca aactgaaata gaactcgtta taaatgttga aataaaactc gccattgtcc
      841 agttcactcc cttgaagcgg tacgagaagc gcgacctggt aacgaagcct cgtactgaac
      901 ggtgtctgac ctgtaactcg attataaata aatctttctt cgaaacgcac tcgatgggta
      961 agacgtccga agatggaatc cgcaccatag acaatctgct ggaagggccg ctgctcattc
     1021 acatagctgt cggaaaaagg attggttcgt tgatagatat accctaggaa aacgaaatta
     1081 agattcgggc tgtatttata gatggggctg gcttggaagt aatgttgtat atctcctgga
     1141 aactcgttat tgctcaagtt ggaattattc gagctgaaaa cattaaaatc ccatcgatca
     1201 gaaacaacct tggtaagaga tatctgcggc cgaaaaccat atagctgctg cgtctctgcg
     1261 gatatcggat tgccgaagat cagaagcatg acaaatacaa gctttttctt catcacagcg
     1321 ctcgatcaat ggctaacggt tcgagttggc atgaaccata atgccaggta gaagaaggca
     1381 gtgatgctgg aaacgagcag cccccgtctg cgcctccctc cggtcagctt ctactggcat
     1441 tggctgacct tttgatgatg gaatttcgat cgacgagcga ggaaagccga gactgcctgc
     1501 gagcatgaat gaaagaagtg ggatgaaacc gatttcatga tgcctctggt ttatagctgg
     1561 ttgcagaatg ttgccatccc ctataagcaa ctcgcgtgcc ataaaaaaag acatctcaaa
     1621 aacagttctt taacgtctta caaactatac attgtccctc ccaaaggaca agcacttgtc
     1681 cgctttagga acatcgctga cccttctttc agccgggcaa gcactgctcc ctggaagctc
     1741 gcgtttgtcc aggcggttcc cggcctcttg ccaagcgtct cgttcgccta tttcttgctt
     1801 agaaattcag ttaaatgtcg gaatagactt catttttata ttgataaatt cccgcaatgc
     1861 ccccgtatgg agttcgaata ctgcttcgac aaggcccgtt tttgattcag ggtgagagaa
     1921 aaaaatttcg agcagtacct cgttgttcct gtaaagctct tcaaccatca aagtcttgta
     1981 accggggtaa cgagcagcaa gaactttttc gatctttttt cttgtagcag ggggtatatc
     2041 ggaaagcttt attctccttt cttgttccaa tatcttccct tggggatcat aggtaattgc
     2101 aaactggttc ccgccttttt cataatatac gtcatagctg acgagaccct ctttcagctt
     2161 gcgtttataa gtaaattccc gctcttcgta tcctttcggt aaagccatca cgactggatg
     2221 aggcaactcc tttttgcgta tgacttcttc ctcctcggtc gaatgagggg tttcaggcag
     2281 gatcgatgca caggcggcga aaaaaaagac agacgaggca gccaacacga ttgaaatgcg
     2341 agacatcatt ttttttgcgc gtggaaatga cattgaccga gcttgatctg gcctgcggtc
     2401 agtcatacag gcggttgatg ttgaggccaa tgtcaggaaa atatgagacg ctcacctcac
     2461 cttatcagtc gaaattatgc gaccagctaa actgcaggat attgaaattc cgtatatcgt
     2521 gcttatcgac aaccactctc tgaagcaagg ggcctatttc aatccttcca tacttcgcgg
     2581 tctggtagcc aatacctgca taagcccaat tttcattata agtcgcgccg ctgggacctg
     2641 aggtaatgaa ataaaattca ttataaatat tgaaatagaa ttctcccttg ttcaattctt
     2701 cgccttgcaa tggcaccaga agcccaagct gataacggag tctcgtaccg aggaacttct
     2761 gttcgggcgc tttatcgtgg atgaatctct gttcgaagcg cacccggtgc gtcacgcgtc
     2821 caaagccgaa atcggtacca tagacaacct gctgaaaaat ccgattctca ttgaaaaaat
     2881 tatcaaaaac aggactggtt ctttgataaa tgtagcctaa aaaaacaaaa ttaagattcg
     2941 gactgtattt gtaagtagga ccgaccagaa aataattttg tacgttggta ggtggctgtt
     3001 cccctccgta tgttttatcg acgaggtttg cattttccga ggtgaagatg tttaaatccc
     3061 aacgatcgaa aacttgcttg gtaagggata tctgggttct aaactcatag aattcatgcg
     3121 tatcacccgc atataaacga ttgccgacga tcataaacat gataaataca aatatcttca
     3181 tgtcattcac ccgctgtctc gagttaaacc gctgattggc tcttgattca gatggctggc
     3241 ggggcggttc ggtcggcagc cggcatgaaa gtcaggaaaa ggacggcact cccgaatgaa
     3301 ccgggctttg tattgatctc cctcggtcca tttgctcgcc gtacaaaaac ttcgctacgc
     3361 cggatgattg cctatcctga ggataggcag gagttcaagc ttctttgccg ctgcccaagg
     3421 ctccgatcaa tgatgccgct gagaagcatt tagcaatggc ttgagcgatg ccaggctgat
     3481 gccgacggca aacgcctcaa gtgaacgccc gtaccgggtc tcggtacctc ggccctttgc
     3541 agcaaatggc gcgccagtat gcacgagcaa ataggcgtca taggcagtgt cttttggcaa
     3601 tcgaacattc gtgccgtttt caagtactac gatcctgggc tcgccgtgct tgccggaaat
     3661 gatccgctgg atttttcctt tcgcgctgag ttccttgagc tcggcagcac gcaattcttt
     3721 cggcatcacc ttgccattcc ataacggctt ccggctcaga agcgtctggt tcgattcgat
     3781 attcgtgatg gagtaggctt caaagctcga tccgccttct ggcgcacctt gcaaagcaac
     3841 gatatcgccc ggcttgacca actctgcgac gtctcgcgac atatgcggag gcagttttac
     3901 cagcgtgccg ttgtcaagga ataatccatc gacctctcca aacggattga tgagatagcg
     3961 taccgctttg ccttgcaggc cggtcagggg ttgctgggtg tgctgctcat tcactgcgta
     4021 ggcagccggt gcggaaaaaa gaaagctcgt aagcataaag gccagaacag cgttgtatat
     4081 agcttccatc gtaatctccg gtttatcggt tgacaggaac acctaatcct tctgtagcaa
     4141 cccctgtgcc aacaaggaag atacgtgaaa acagaagctt ggaaagaact ggatagcatg
     4201 ttgtccttta cagggacgag acaacgtccg cattacggac attaatccat gcgctatcac
     4261 tcggcttgag tacaccagtt tcaagacccg ggccgactct cgatagcgct cgtaagtaaa
     4321 acggggaatg tggtctaatg gcagaaagcg gcgatagtag ggtttgcttt ctggcctgag
     4381 tttttcgcct tgagaggttg atccggcgga atgcgaatca ggtggcggct gtgatcatcg
     4441 cagatgcaaa cggctgcggc gctctgttgc cagtattcaa gacctggcgt caagtgccgc
     4501 acgctctcat atggaatttg gcgcgccgag tcgtggaagt tatggagaag cagcgttcaa
     4561 gtgcttctcg aggattttca gtaaagcgtt tgaatagatt tcattcttat atcgacgaat
     4621 tccctgaaaa ctcctgtctt gaattcaaaa acggcttcca caaatccggt tgaagatttg
     4681 ggagcagaga aaaaaacctc tagcagcatt ttgtcaggcg tataaacttc ttcaaccagc
     4741 aatatcttat gatcggggta atgctcggat aagactttct caacctttgc tctcaagtcc
     4801 tgcggtatat cagaaaattc cacctttttt tcttcttcca atatcctacc ttgctcgtca
     4861 tagccgatcg agaatttctt tccacctttc tcataatcca cgtcatagct aatcttgcca
     4921 ttcttgtgct tgcgcttata cgtgaattct ttcttgtcat actctttcgg caaagccgct
     4981 agaactcgga gagggaggtc ttttacacgt attgtctcct caacttccgt cttatgataa
     5041 gtagccggta ccgtcgccac acacccattg aggaaaacga gagatgaagc gaccaatact
     5101 ctcccgatag gagacatgac gttcctttct tttttatgtt tgatccgagg atcaaaggcg
     5161 agattctgta cccaaggcac ggccggccaa cgaaccttgg aaatgtagcg cacgccgtgc
     5221 ttatggacaa gccgggctgc tgaagggttt gtgatttggc gggccggtaa ccgaaaatcg
     5281 gttacgattt atgaaaccat aatagtaaac tgtgtgcaaa aatcgggaca agtaacgatc
     5341 gctatccaat tcgcctgacc ccctgagttc tcgcgctcaa tttccagcaa gtggttattt
     5401 ttggccagat gctccaatac gtgctgacgg tcttgatttc gcccctgacg catcggacgt
     5461 agcttcaaga ttgagggctg gacgggccca ttgcactgcg tgggcccgtc tgtcaatggt
     5521 atgtcacgtt gtaattgaac agattgcctg ccgattattg ttaacgcttc tgatacacag
     5581 gctgaggctg ctcacccgct ggagcaagct cagtggcttc cagtgcctgg ccgtactgat
     5641 ttttagtgcc gtaaccggat actgtaatac tctgtccgat ttttaacata ggcgcgaatt
     5701 gataagatgc ctcgtgcggg aagcgaaccg tcgtgccatc cgacaggatt accccattaa
     5761 tctcgccgcg cttgccataa tgaagatgac ggatttcacc ctgcgcattc atggcccgca
     5821 agctaacgct ttgcaagtgc tttggcatct tcatgactcc cttggggggt tttggctgaa
     5881 ccattaaaga ttcgccactg cgcgaattgg taatgacata acccttgatc tggcctgccg
     5941 cctcagggta acccttgatc tcgactgtat ctccgggctt gactgcagct gccagctgct
     6001 cgcccatatg ctttgggaaa gtgacgagat tgccagtatc gaggagcaga ccatccactt
     6061 caccgaatgg attgacgagg aaacgggtca ccttgccttg agcagcgggt gccgcagtag
     6121 caaaagcttg gacgcggagg aattcgtcgg ctctaacgtg tcccttttta gcttgagcga
     6181 ccggtgtgat agatattgcg gacatcaata aaacaggtaa taagtgacaa gttctcatgt
     6241 tcatctccag ttataggagt tgcgtaaggg cagtgcacaa gctaactatg caagccccgt
     6301 gccatgggcc aggagattga ttgataagga ataccgggag taatgctatt gccaactgtc
     6361 cccttagggg acatcgaagt tcgcgtaacg gacaaatttc gtcttagggc tcgatcccgt
     6421 actgccgcag tttcgcatag agcaattgac ggcggatgcc cagccgtcgc gcagcctcgg
     6481 tgcgattgcc gtccgtttcg gcaatcgccg cgcggatcat catttcttca agctgtgcca
     6541 gcgccgtggg aaggtcatgc ataagtggcg ctcctgccgg taacgcgaga ggcgcaggag
     6601 ttctaaccag gccaaggtcc gcaacgtcga tcgaagcgcc ccgcgcgaga atcattgctc
     6661 gttcgattac attgcgtagt tcccgcacgt tgcccggcca cgtatgcgtt tccagacatt
     6721 gccgggcctc tgccgaaagc gcttttggcg tatcgcttgc cagagcaaga aaatgctctg
     6781 ccaacgcgat gatatcctcg cgccgttcgc ggagcggcga tacgtggata ttgatgacgt
     6841 tgagccgata aaacagatct tcccgaaacc tgccttccgc gacctgctgc ccaagatcac
     6901 gatgggtagc tgcgagcaga cgcacattga ctgacagggg ccgctctgcg cccagcggca
     6961 cgatcacgct ttctgccaga acgcgcagca gcttggcttg cagattgccg ctcatatcac
     7021 cgatttcatc gagcagcaag gtgccgccct cggcctgagc gaagatgccg acgcgagatg
     7081 cagtcgcgcc ggtaaaagct cccctgacat gaccgaacag ctcgctttcg agcagttcgt
     7141 ggggaattgc cgcgcaattg atggcgacaa aaggatatga tgcccgagcg ctgtactgat
     7201 gcagggcacg ggcaattatt tctttaccgc ttccagtttc gccggtaatc agcaccgtcg
     7261 cgtcgctggc tgctgcacgt ccgatcaatt taacggtttc tcgcatgcgc ccgctgcgtg
     7321 cgacaaactc gccttcgccg ggaactggat tttgtcgtat tgccctgctg ctggaagcca
     7381 aagctgcgtt ca
//
LOCUS       DASSHJ010000262         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1502636,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000262 DASSHJ010000000
VERSION     DASSHJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502636"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1820)
                     /locus_tag="VFI43_10155"
     CDS             complement(183..1820)
                     /locus_tag="VFI43_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062527.1"
                     /translation="MRCSSSLILLSGCLLSVPLQAEDKPPSTAGTDSAAAPAYVIDLY
                     VDTKTKQIYSEPGEGRVRMGSFEKVSDQKPAPKAGAEAGGAGLPASAASGAVAAAAPK
                     PQGMGYANWKSPDPFKWNLNPDGSQYLKFGFLNQVWLRYEQNNPGSQVLGDPVKDTQD
                     IGLRRTRFVLQGQLTDRVYFYTQYGMNNFNFLTQDASTQHNGSDTPIGNRKIQAFFHD
                     AFGELRLTKGHQMIVGAGLVMANGASRFSSPSVSTIMTMDIPLFAQFTADRTDIFNRS
                     LSVYLRGQIGMFNYRFTASDPFPINTSGTGFETISPTMAQFARQGHHKRYQGFYAINF
                     WDTEPMTNPYWQGTYLGKKSILNLEGGFITQKAATWTGTPVAGGGETNVQFHDMTIWS
                     LALYLDAPVDKVKETAISAYLGYFDTNYGPNYLRMNGIGMAPATGGQGPFATGGNQFP
                     MFGTGKLFYGQIGYLLPKDLLGSNNGQLMPYFSWTNADYQALQHRVDVFNVGVNWLIK
                     GHNSKVTFDYQNRPVFNNAGDKIANRGQFVLQYQIAF"
     gene            complement(2653..3057)
                     /locus_tag="VFI43_10160"
     CDS             complement(2653..3057)
                     /locus_tag="VFI43_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062528.1"
                     /translation="MKHILALRNHGISAILAGLLIAGTASMTHAESGGNTPAASGEKQ
                     APSGWNKTETSGGGWLSSIFGGSAEASAPDKAETSSGWSSPRDGSGTCKHSSAEEEDF
                     VCKLVRIFWGTDRPYGPNRDMDDNVAAGGAGG"
     gene            complement(3480..>4103)
                     /locus_tag="VFI43_10165"
     CDS             complement(3480..>4103)
                     /locus_tag="VFI43_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HET7062529.1"
                     /translation="RANLDDPSASTLSVNGDTYTLSQFHFHAPAEHLENGYKYPMEMH
                     LVFQDANKHLLVVGRWVQEGSFNQTLDPIFSHIPQDTSTTLTIDHFNLNALLPDHLNS
                     FRYDGSLTTPKFDEGVKWVDLSTPMDMSKEQINAFSSLFPNGDAREVQALNGRIVQTD
                     LPGFTVTSVPEPETYAMMLVGLGLIGFIANRRRRMNSLQSGVLPAAV"
BASE COUNT          924 a         1065 c         1127 g          987 t
ORIGIN      
        1 agggacgaag actcgcgtaa gtcctcccct tcgcgggaca caggggtatg gggggcggaa
       61 aaagaaaact ctcgggatag ggcccgagag gcagaggatt gataaggagg cggaaattac
      121 ttcaggcagg ccctagtcgg cctgcctgag cttgaaccct tgagattgcc ggattgcaat
      181 gattagaaag cgatttggta ctggaggaca aactggcctc tattggcgat cttatcccca
      241 gcgttgttga acacaggtct gttctggtaa tcgaacgtta ccttgctgtt gtggcccttg
      301 atcagccagt ttacgcctac gttgaacacg tccaccctgt gctgcagcgc ttggtagtcc
      361 gcgttcgtcc aggagaaata gggcatcaac tgcccattgt tgctgcccag caagtcttta
      421 ggcagcagat agccgatctg cccgtagaaa agtttccccg taccaaacat ggggaactgg
      481 ttacccccgg ttgcgaacgg cccttggccg ccggttgccg gagccatgcc gatgccgttc
      541 atgcgtagat agttcggacc gtaattcgtg tcgaaataac ccagataggc ggaaattgca
      601 gtctccttga ccttgtccac cggtgcgtcg aggtagagcg ccagggacca gatggtcatg
      661 tcgtgaaact ggacgtttgt ttcaccgccg ccggcgaccg gcgttcctgt ccaggttgca
      721 gctttctgcg taatgaagcc gccctcaaga ttcagtatgc tcttcttgcc caggtaggtt
      781 ccctgccagt agggattcgt catgggttcg gtatcccaga agttgatagc atagaagccc
      841 tggtagcgct tgtgatgtcc ttgccgggcg aactgggcca tggtgggcga gatggtctcg
      901 aaaccggtgc ctgaggtatt gatggggaaa gggtcgctgg cggtgaaccg gtaattgaac
      961 atgccgatct ggccgcgcaa atagacgctc aagcttctgt tgaagatatc ggtcctgtcg
     1021 gcagtgaact gcgcgaacag cggaatgtcc atggtcatga tcgtgctgac gctggggctg
     1081 ctgaaacgcg atgcgccatt ggccatcacc aaacctgccc ccacgatcat ctggtgaccc
     1141 ttcgtcaggc gcaattcgcc aaatgcgtca tgaaaaaagg cctggatctt gcggttaccg
     1201 attggcgtat ctgaaccatt gtgctgggta ctggcatcct gggtcagaaa gttgaagttg
     1261 ttcatcccat actgagtgta gaaataaacg cggtcggtga gctggccctg caacacaaaa
     1321 cgtgttctgc gcaacccgat atcttgcgta tcctttaccg ggtcacccag cacctgcgag
     1381 ccgggattat tctgctcata ccttaaccag acctgattca ggaagccgaa tttcagatac
     1441 tggcttccat ccggattcag gttccattta aagggatccg gcgatttcca gtttgcgtag
     1501 cccattccct gtggtttagg cgctgcggca gcgacagcac cacttgccgc gctggcggga
     1561 agcccagctc ctccggcttc agcccctgct tttggcgcgg gcttctggtc cgataccttc
     1621 tcaaaagaac ccattctgac ccgcccctct cccggttcag aatagatctg cttggttttg
     1681 gtatcgacgt atagatcgat tacataagcg ggtgctgccg ccgaatccgt acccgcggtg
     1741 gatgggggct tgtcttcggc ctgaagggga accgaaagta agcagcctga cagcaatatc
     1801 agcgaagaac tacacctcat tgttatgatc tccctattta gagttatgcc aatcgtttta
     1861 gctacttatg ctgggcaaaa gtagaaaaag ctgagtttat gaattaaaaa atagcacgtg
     1921 atgtgtcaaa aaatcgtcag caagaagtga agaaaacgtg agagacgcgg cgataaaata
     1981 ccataaaaag aaatttgcga atcggcattt cccatgcgta aaaggttaaa tatctcaggc
     2041 catccgagaa atgttgtttt tatcaaaaag aacgaggcgc acggctcata tcctgctgag
     2101 caggatcggg aaccacggat gcagcccagg gcttagcccc cggcagcgcc acccgcctgg
     2161 atatttgtat ccacgtcagg atctggtccg caccagtccc ggctgaagag caaccgggtt
     2221 actttatgga aaaagttttc gttttccggt attggaggac cgcaccagcg ccgtcctcta
     2281 ataggggaag accagcccga ttctgcctct ttttgatcag gacgcacctc gttggaagta
     2341 gcggcctggg cagcggcgga tgttgcccgg gacccggcag gtgccttgac attgtcccct
     2401 ggggcgggac acagctgagc gcaggaagtc agcgcaagac ataaaagcag cgttccgact
     2461 gcacttgagg atccggaagc attgcatctc ataaaaacac acattcgttt tcctttccgc
     2521 gcatcgaatg atcgatgcaa ggggcttgta tgcgatgctc ttcttacctg ctttttatcg
     2581 cgcttctaca aatgcattac cacgaatttt tccaggtagc cgcatgtaga tgaaacggcc
     2641 acagagagac gtttagcccc cggctccgcc ggctgcaaca ttgtcatcca tatcccggtt
     2701 gggcccgtac ggccggtcgg taccccaaaa gatcctgacg agcttgcata caaaatcctc
     2761 ttcctcggcg gatgaatgct tgcaggtgcc acttccgtct cttggggatg accaaccgga
     2821 tgacgtttcg gccttgtcag gcgcgctggc ttccgccgag cccccgaaga tcgacgataa
     2881 ccagcctcct ccactggtct cggttttatt ccagcccgag ggcgcttgtt tttcgcctga
     2941 agcggcggga gtattgccgc cagactctgc gtgggtcatg ctggcagtcc cagcgataag
     3001 cagtcccgcg agaatcgcgg agattccatg atttctcaac gcgaggatgt gtttcatgat
     3061 attcctccct ctgtttgcgg gccgatagcg gccagcggtg gaaaataatc tattctattt
     3121 cgcctggaaa attttcggtg aaaaaaattg aactttaccc acatttttcc gtcgccgcca
     3181 cttccattcc gggagcgatt tttcatggag ttgcgtggag tggatgaaga tcgagatcgg
     3241 gctggagaac aggattgctg gttttaaaga atttccgatg aacggatgaa gagggagctc
     3301 ttttctacac ccactgagag tgcttttcca ggactgccgg ggctattaaa agttatattt
     3361 ctgtcgtacg aacttcttct gctaattacg cccgctattt gtttccgtta tgcctgattc
     3421 ccccaccttt cctgatggtg ggggaatcag gccggaagcg ctttatgcga caggaggcac
     3481 tataccgctg caggcagaac tcctgattgg agcgagttca tccgtcttct acggttcgcg
     3541 atgaagccga tcaggcccag acccaccagc atcatggcat aggtttccgg ctccggaacc
     3601 gaggtgacag taaatccggg taggtccgtt tgtacgatac gcccgttgag cgcttgaact
     3661 tcgcgagcat caccatttgg gaacaacgag ctgaacgcat tgatctgttc ctttgacatg
     3721 tccatcggtg tcgacagatc gacccatttc acgccttcgt cgaatttggg agtagtaagc
     3781 gagccatcat agcggaagga attcagatgg tctggaagca gcgcgttcaa gttgaaatgg
     3841 tcaatggtga gtgtggtcga cgtgtcctgc gggatatggg agaaaatggg atccagcgtc
     3901 tggttgaagg agccctcttg tacccagcgg cctaccacga gcaagtgctt gttcgcgtcc
     3961 tgaaatacga gatgcatctc cattgggtat ttgtagccat tctccagatg ctcagcaggc
     4021 gcatggaaat ggaattggga taacgtgtaa gtatcgccgt tcacactgag cgtgctcgcg
     4081 ctcggatcat caagatttgc cct
//
LOCUS       DASSHJ010000263         9617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_708412, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000263 DASSHJ010000000
VERSION     DASSHJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708412"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFI43_10170"
     CDS             <1..825
                     /locus_tag="VFI43_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7062530.1"
                     /translation="VGAYISGAIPGKGMNAQQMLGVSGDEACRAYLLMNIEPELDSYN
                     PQRTIKALDEAELVVMLSSYKSPHAVSGNYADVLLPVAPFTETSGTFINTEGGVQSFN
                     GVVPPLGETRPAWKVLRVLGNMLGVEDFDYDTPEQVRAEILPAGGDIKGRLANDLKGF
                     VAAIGNGRSQTQTSGTQRIGEVPIYQADPIVRRAESLQRTHDAAPPVAWMADALMEKL
                     GISAGDEVRIRQGDGEAQLPAVRDNRLPEGCVRVAAAHPLTAALGGMFDEITVEKL"
     gene            861..1961
                     /gene="nuoH"
                     /locus_tag="VFI43_10175"
     CDS             861..1961
                     /gene="nuoH"
                     /locus_tag="VFI43_10175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET7062531.1"
                     /translation="MEHFQQIFGDFFGPEWGPALFLVAKNVLLIVAIILPLMLAVAYL
                     TFAERKIIGYMQLRVGPNRVTFFGIPWLGGWGQPIADAVKAVMKEIIIPSGANKFLFV
                     LAPVLTFAPALAAWAVVPFSPEVVLADINAGLLYILAMTSMGVYGVIIAGWASNSKYA
                     FLGAMRSAAQVVSYELAMGFALVCVLMMSHSLNLGDIVRGQQGATFISWYLIPLFPMF
                     LVYFISGVAETNRAPFDVAEGESEIVAGFHVEYSGMAFTVFFLAEYSNMILVATLASI
                     IFLGGWLPPLDMAPFTLVPGFIWLLLKVSFLLFCFLWFRATFPRYRYDQIMRLGWKVF
                     IPITLVWIFLIGLVMQFPPAMLNAFPLNLWFH"
     gene            1975..2463
                     /gene="nuoI"
                     /locus_tag="VFI43_10180"
     CDS             1975..2463
                     /gene="nuoI"
                     /locus_tag="VFI43_10180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET7062532.1"
                     /translation="MNRIKDFFRTFLLFELVKGMMLTGRYLFARKITVQFPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAICPALAITIESEQRDDGTRRTTRYDIDLTKC
                     IFCGFCEESCPVDSIVETRILEYHGEKRGDLIYTKQMLLAVGDRYEEQIANDRAADAG
                     YR"
     gene            2484..3089
                     /locus_tag="VFI43_10185"
     CDS             2484..3089
                     /locus_tag="VFI43_10185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET7062533.1"
                     /translation="MSFPDIIFYFFSAVLVLSALGVITARNPVHSALLLVLAFFTSAG
                     LWLLLEAEFLAITLVLVYVGAVMVLFLFVVMMLDINLDQLREGFWKWFPFGAFIALLM
                     AGEMAIVLMGRQFGIDEMPVPAPRGDDYSNTKELGRLIYTEYVYAFELAAVILLVAIV
                     AAIALTLRQRTGIKSPDVSQQVAVKRADRIRMVSMPAEKKE"
     gene            3156..3461
                     /gene="nuoK"
                     /locus_tag="VFI43_10190"
     CDS             3156..3461
                     /gene="nuoK"
                     /locus_tag="VFI43_10190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET7062534.1"
                     /translation="MISLSHYLVLGAILFAISIVGIFLNRKNVIILLMAIELMLLAVN
                     MNFVAFSHYLQDVSGQIFVFFILTVAAAESAIGLAILVVLFRNLRTINVDDLDNLKG"
     gene            3541..5493
                     /gene="nuoL"
                     /locus_tag="VFI43_10195"
     CDS             3541..5493
                     /gene="nuoL"
                     /locus_tag="VFI43_10195"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7062535.1"
                     /translation="MTEMQKLYLFVPLAPLAGALIAGLFGRLIGPVWSHRATIILMFM
                     CVGASITVFMDVLGGNVYNGTVYTWMTSGNTRFEVGFLIDRLTATMMIVVTVVSLMVH
                     IYTIGYMRGDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWY
                     TRPTAIYANLKAFLVNRVGDFGFLLGIGLVLAYFGTLDYETVFAQAPLAVGQTIEIFS
                     GTPWALLSVICILLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMV
                     ARMSPLFELTETALSVMLVIGGITTFFMALVALVQNDIKRVVAYSTLSQLGYMTIALG
                     ASAYSAGIFHLMTHAFFKAVLFLGAGSVIIAMHHEQDMRKMGGLKKYMPITYWTMFIA
                     ALASAGVPGFSGFFSKDAIIEAVHFANIPGAGFAYFCAVSTVFVTAFYTFRLMFMTFH
                     GKPRMDHHTEEHLHESPWVVTLPLVILAIAAIGAGWLIEPMLFGGYFGDAIRILPQHD
                     AIAQMAAQFHGIGGMMAHALTTLPFWLSAAGIFLAWYLYIVNTALPAKISQAAGPLYT
                     LLDHKYFIDELYSLLFARGSRALGQGLWKIGDVTIIDGFFVNGTARMVAGASMLMRRF
                     QSGYIYHYAFTMIIGVFVLMSLWLLP"
     gene            5559..7043
                     /locus_tag="VFI43_10200"
     CDS             5559..7043
                     /locus_tag="VFI43_10200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET7062536.1"
                     /translation="MLFGFPLLSLIIWLPIVAGIGVLATGGDRNAQMARWIALVGATV
                     GFLVAIPLYTRFDPSTNAMQFVEHSVWIERFNIHYHLGVDGIAMPLILLNCFITPLVV
                     IAGWEVISKRVSQYMGAFLIMSGIVNGVFSALDAILFYVFWEASLIPMFLIIGIWGGP
                     NRVYAAIKFFLYTLLGSLLMLVAFIYLYQVSGGSFSVLDYHRLPLSLAAQVLIFIAFL
                     LAFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAILLKLGGYGFLRFSLPIAPDASHHL
                     SGFMIVLALIAVVYIGFVALIQADMKKLIAYSSVSHMGFVTLGFFLFNAYGIEGAMLQ
                     MISHGFISGAMFLCVGVMYDRLHSRQIADYGGVVNKMPVFAAFFMLFAMANSGLPGTS
                     GFVGEFLVIMASMKVNFWYAFFAAISLMTGAAYTLWMYKRVVFGAVVHPAVEGLDDVN
                     GREIFVLAVLALAVLWMGLYPLPIVEVMHTTVDDLLAHLARSKL"
     gene            7077..8525
                     /gene="nuoN"
                     /locus_tag="VFI43_10205"
     CDS             7077..8525
                     /gene="nuoN"
                     /locus_tag="VFI43_10205"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HET7062537.1"
                     /translation="MNFLQPDLAPAYPEIFLLLMVCVVLLADLAWGKEKPHLAYLLAQ
                     LALFGCVLITFATSAPGVAYTFSGMFVDDPLADILKMMVYVTVSVVLVYSRTYISARG
                     LLSGEFFSLSLFATLGMMVMISASHFLTLYLGLELLSLSLYAMVALRRESVGATEAAI
                     KFFVLGALASGFLLYGMSMVYGSTGSLNIARVAEVIQSGTVSHTVLVVGVVFIVAGIS
                     FKLSAAPFHMWAPDVYEGGSTAAVLFIGSAPKLAAFGFIMRLLAEGLGPMVADWQGML
                     IILAVMSMAIGNIAAIAQTNIKRMLAYSTISHMGFVLLGVLAGDGNGYSAAMFYVVVY
                     VLMTLGTFGVIMLLSRTGFEAEMLDDFKGLNRRNPWYAFMTLLLMFSMAGIPPTVGIY
                     AKLSVLQAVLSAGYTWLAILAVLLSLIGVFYYLRIVKLMYFDEPESDAAISPQGDVKL
                     LLSANGLAMLAFGLFPQALMAWCAYAIQQSSI"
     gene            complement(8671..>9617)
                     /locus_tag="VFI43_10210"
     CDS             complement(8671..>9617)
                     /locus_tag="VFI43_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET7062538.1"
                     /translation="ALGQNGTVFAGSSSEGNVYAITETEGGRKVKTIASGLSMPIGVA
                     FKDGALYVSAISRILKFDGIEGKLDEPGKPTVIFDGFPTEKHHGGKFIAFGPDGLLYV
                     PVGAPCNICEPDPEKFALISRMKPDGTGHEVFAQGVRNTVGFDWHPQTKELWFTDNGR
                     DWMGDNLPPDELNRAPRKGMHFGYPYCHAVDILDPKYGAKRDCSRSTPPVTKLDPHVA
                     PLGMRFYTGKMFPPEYRNNIFIAEHGSWNRRNKSGYRLQFARIKDNKVVNQEVFAEGW
                     LEQENAWGRPVDVLVMPDGALLVSDDYAGVIYRISYRK"
BASE COUNT         1858 a         2552 c         2681 g         2526 t
ORIGIN      
        1 gtgggcgctt atatttcagg cgcgattccg ggcaagggca tgaatgccca gcagatgtta
       61 ggtgtgagtg gggacgaggc ctgtcgtgca tatctgctga tgaatatcga acccgaactg
      121 gatagttata atccgcagcg aacgatcaag gccctggatg aggcagagct ggtggtaatg
      181 ctgtcctcat acaagagccc gcatgcggta tcgggcaact atgccgatgt attgctcccg
      241 gttgcgccat ttaccgagac ctccggcacg ttcatcaata ccgagggagg cgtccagagt
      301 tttaacggcg tcgttccacc ccttggggag acccgccctg catggaaagt tcttcgtgtc
      361 cttgggaata tgcttggggt cgaagatttt gattacgata cgcccgagca ggtccgcgca
      421 gagatccttc cggctggagg agatatcaag ggtcgcctgg caaacgatct gaagggtttt
      481 gtagcagcta ttgggaacgg caggagccaa acgcaaacct ccggaactca gcgcatcggc
      541 gaggttccga tctatcaggc tgatccgatc gtaaggcggg cagaatccct gcaacgcacc
      601 catgacgcag cgcccccggt tgcgtggatg gcggacgccc tgatggaaaa gcttggaata
      661 agcgcagggg atgaggtgcg aatccggcag ggggatggag aagcccagtt gcccgcggtg
      721 cgagacaaca ggcttccaga aggatgtgtc cgggttgcgg cggcccatcc cttgactgcc
      781 gcattgggcg gcatgttcga tgaaatcacc gtcgaaaagc tctgacaaag aaagaatgtg
      841 aagaaaggcc aggtcgtttg atggaacact tccaacagat attcggagat ttttttggtc
      901 ctgagtgggg acccgcgctt ttccttgtgg cgaaaaatgt gctgctgatc gtcgccatca
      961 ttctgccttt gatgcttgcc gtggcctatc tcacatttgc cgagcgcaag atcatcggat
     1021 atatgcagct gcgcgtcggg cccaaccgcg tgactttctt cgggataccc tggctgggtg
     1081 gatgggggca gccgatcgcg gatgccgtca aggcggtgat gaaggaaatt atcatcccga
     1141 gcggggcgaa taaattcctg ttcgtgctag cgcccgtcct gacgtttgcg cccgccctgg
     1201 cggcctgggc ggtcgtgccg ttttctccgg aggtggtact cgccgatatc aatgccggcc
     1261 tgctttatat attggcgatg acgtccatgg gcgtatatgg ggttatcatt gcgggctggg
     1321 catccaattc gaaatacgcg tttctaggag cgatgcgttc ggccgcccag gtcgtttctt
     1381 atgagctggc gatgggattt gcgctggtgt gcgtgctgat gatgtcgcat agcctgaacc
     1441 tgggggatat tgttcgcggc cagcagggcg ccacctttat cagctggtac ctgattcctc
     1501 tttttccgat gttcctggtg tacttcatat caggcgtggc cgagacgaac cgtgcgccat
     1561 tcgatgtggc tgaaggtgaa tccgaaattg tcgcgggatt tcacgtcgag tattccggta
     1621 tggctttcac ggtatttttt cttgcagaat actcgaacat gattcttgtg gccacgcttg
     1681 caagcatcat attcctgggc gggtggctgc cgcctctcga catggcccca tttacattgg
     1741 taccggggtt tatctggctg ttgctgaaag tgtcgttcct gctgttttgt tttctctggt
     1801 tccgcgccac atttccacgc tatcggtatg accagatcat gcgcctcggg tggaaagtat
     1861 tcataccgat cacgcttgtc tggatatttc tcataggctt ggtgatgcaa tttccacccg
     1921 ccatgctaaa cgcatttccg ctgaacttgt ggttccactg agacaggaga agccatgaac
     1981 cgtatcaagg atttcttccg tacctttctg ttgttcgaac tcgtaaaagg aatgatgctg
     2041 accggccgct atctgttcgc gcgaaaaata accgtgcagt tccccgagga aaaaacacct
     2101 cagtcccctc gtttcagggg gcttcatgca cttcggcgct accctaacgg agaagaacgc
     2161 tgcatcgcct gcaaactctg cgaagcaata tgccctgccc tggccatcac catcgaatcg
     2221 gagcagcgcg acgatggcac gcgccgcacg acgcgttatg acatcgacct caccaagtgc
     2281 atattctgcg gattttgcga agagtcctgc ccagtggatt caatcgttga aacgcggatc
     2341 cttgaatatc acggggagaa acggggggac ctgatatata ccaagcagat gttgcttgcc
     2401 gtgggagacc ggtacgaaga acagatcgca aacgatcggg cagcggacgc aggctaccgg
     2461 taaacctcat cccataagaa cacatgagtt ttccagatat cattttttac ttcttctcgg
     2521 ctgtgctggt gttatcagcg ctgggagtca tcaccgctcg caatcccgtt cattcagcct
     2581 tgctgctggt tcttgccttt ttcacttcgg cgggactgtg gctgctcctt gaagccgaat
     2641 ttctcgccat taccctcgta ttggtgtatg taggcgccgt catggtgttg ttccttttcg
     2701 tagtgatgat gctggatatc aatctcgatc agctacggga aggattctgg aaatggtttc
     2761 cctttggcgc attcatcgcc ctgttgatgg cgggcgagat ggcgatcgtg ctgatgggca
     2821 ggcagtttgg aattgatgaa atgccggtgc ctgcgcctcg cggcgacgat tacagcaata
     2881 ccaaggaact gggccgcctg atctataccg aatatgttta cgcgttcgag ttggcggcag
     2941 tcatccttct cgttgctatc gtggcagcta tcgcactgac tcttcggcaa cgcacaggca
     3001 tcaaatcgcc tgatgtttcg cagcaagtag cggtcaagcg ggcggatcgg atacgcatgg
     3061 tatcaatgcc cgcggaaaag aaagaataag gcagaaagaa caagacattg taaacggtca
     3121 atcgccgata ttgacctgaa aagaggggct ctcgcttgat ttcgttatcc cattacctgg
     3181 tcctgggggc gattctgttc gccatcagta tcgtcggtat tttcctgaac aggaagaatg
     3241 tgatcatcct gttgatggcg atcgaactga tgcttttggc ggtgaacatg aattttgtcg
     3301 cgttctcgca ttatctccaa gatgtatccg gacaaatttt cgtgtttttc atcttgacgg
     3361 tcgctgcggc cgagtcagcc attggtctgg cgatactcgt ggtgttgttc cgcaacctgc
     3421 gcaccatcaa tgtcgatgat ctggacaatc tgaaaggcta atagaggaag agcggcgctg
     3481 tgccgagggg ggtgggacct ccctcgctgg gataacgcaa gtttaaaaag aaagccgatt
     3541 atgaccgaga tgcagaaact ttatctattc gtgccgctcg caccacttgc cggtgcgctg
     3601 atcgcaggac tgtttggacg tctcatcggc ccggtctgga gtcaccgcgc gaccattatt
     3661 ttgatgttca tgtgcgtcgg ggcttcaatc acggtgttca tggatgtgct gggcggcaat
     3721 gtctataacg ggacggtata cacctggatg acctccggga atacgcgttt cgaggtcgga
     3781 ttcctgatcg atcgtctcac tgcaacgatg atgatcgtcg taaccgtcgt ttcattgatg
     3841 gtgcacatct ataccatcgg ttacatgcgg ggcgacccgg gttatcagcg cttcttcagt
     3901 tacatctcgc tttttacttt ctcgatgctg atgctggtga tgtccaacaa cttcctccag
     3961 ctgtttttcg gctgggaagc tgtaggactt gtctcctatc tcctgatcgg tttctggtat
     4021 acacggccta ccgccatcta cgccaatcta aaggccttcc tggtcaaccg ggtaggggat
     4081 ttcgggtttc ttctcggtat cggcctggtt ctcgcatatt ttggcacgct cgactacgag
     4141 acggtcttcg cacaagcgcc tctagctgtg ggacagacta tcgagatctt ctcgggcacg
     4201 ccctgggcgc tgctgtcggt aatctgtatc ctgctgttcg tcggagcgat gggaaaatcg
     4261 gcccagtttc cattgcacgt ctggctgccg gattcgatgg aaggtccaac ccccatctcg
     4321 gcccttattc atgcagctac gatggttacc gccggcattt tcatggtagc ccgaatgtca
     4381 cccctattcg agctgactga aactgccttg tcggtgatgc tcgtgattgg tggaatcacc
     4441 acctttttca tggcgcttgt cgcgctcgtg cagaacgaca tcaagcgcgt agtggcgtat
     4501 tcgacgctgt cccaactggg ttacatgaca attgcgctgg gggcttcggc ttactccgcg
     4561 ggtattttcc acttgatgac tcatgctttc ttcaaggcag tgctttttct tggcgcgggt
     4621 tccgtcatca ttgccatgca tcatgagcag gacatgcgca agatgggcgg gctcaagaaa
     4681 tacatgccca ttacctactg gacgatgttt atcgcggccc ttgcgagcgc gggcgttccc
     4741 ggtttttccg ggtttttctc aaaggacgcc atcatcgaag cagtccattt tgccaacatt
     4801 cctggagctg gctttgctta cttctgtgca gtatcgaccg tcttcgtaac agccttttac
     4861 acctttcggc tgatgttcat gactttccac gggaagccgc gcatggatca tcataccgag
     4921 gagcatttgc acgaatcacc ctgggtcgtg acattgccac tcgtcatcct tgcgatcgct
     4981 gccatcggtg ctgggtggct gatcgagccc atgttgttcg gcggctattt cggtgatgcc
     5041 ataagaatcc tgccccagca tgatgctatt gcacaaatgg cagcccagtt ccatggaata
     5101 ggcggaatga tggcgcacgc actcacaaca ctgccattct ggctctccgc cgccggaatt
     5161 ttcctggcct ggtatttata tatcgtcaac actgccttgc cggcaaaaat cagccaggct
     5221 gccggtccgc tctatacgct gcttgaccac aaatatttta tagatgagct atattcattg
     5281 ctcttcgcgc gaggctcgcg tgcgctcggc caaggcttat ggaagattgg agatgtgacg
     5341 atcatcgacg ggtttttcgt caacggcacg gcacggatgg ttgcaggagc gtccatgctg
     5401 atgcgccgtt tccagtccgg ctatatctac cattatgcct tcaccatgat cattggtgtc
     5461 ttcgtgctga tgagcctgtg gctgcttccc taatagtgga actatcttgt gaaatggccg
     5521 ctgcgtcgcc gcatgccgaa gaataaaacg gaatagatat gctgtttggc tttcccttat
     5581 tgagtctgat catttggctg ccgattgtgg ctggaatcgg agtgcttgcg acaggaggcg
     5641 atcgcaacgc ccagatggca agatggattg cgctggtggg cgccacagtg ggattcctgg
     5701 ttgcgattcc gctgtacacc cgtttcgatc cgtcgacgaa tgcgatgcaa ttcgtggagc
     5761 acagtgtctg gatcgagcgt ttcaacattc actatcatct gggcgtggat ggcattgcaa
     5821 tgcccctgat cctgctgaac tgctttatta cgccgctggt cgtgatcgcc ggatgggaag
     5881 tgatcagcaa gcgggtatcc cagtacatgg gtgcgtttct tatcatgtcc ggtatcgtga
     5941 acggggtttt ttcagcgctt gatgcaatcc tgttttacgt cttctgggaa gcatccctca
     6001 ttccgatgtt cctcatcatt ggaatctggg gcgggcctaa ccgcgtttac gccgcgatca
     6061 agttctttct atacaccctt ctgggttcgc tcctgatgct ggtggcattc atttacctat
     6121 accaggtttc gggcggaagt ttttccgtgc tggattacca caggctgcca ctatcccttg
     6181 cggcacaagt actgatattt atcgccttcc tgcttgcttt tgcagtgaag gttcccatgt
     6241 ggcccgtcca cacctggctg cccgatgctc acgtcgaggc gcccacgggc ggatccgtgg
     6301 tcctggccgc gatacttctc aagctcggcg ggtatggctt cctgcgcttc tcgttaccca
     6361 tcgcgccgga tgcgagtcac cacctttcgg gattcatgat cgtacttgcg ctgattgccg
     6421 tcgtgtatat cggatttgtc gcgctcatac aggccgacat gaagaagctt attgcgtact
     6481 catcggtatc gcatatgggt ttcgtcacgc tgggcttttt tctcttcaat gcttacggta
     6541 ttgaaggcgc gatgcttcag atgatttcgc atggcttcat atcgggcgcc atgtttctgt
     6601 gcgtgggcgt catgtacgac aggctgcatt cgcggcagat tgccgactat ggcggggtcg
     6661 tcaacaagat gcccgtgttc gcggctttct tcatgctgtt tgccatggcc aactcgggct
     6721 tgcccggcac aagcggcttt gtcggcgaat tcctggttat catggcgtca atgaaggtga
     6781 atttctggta cgcattcttc gcagcaatca gcttgatgac cggggcggct tacaccttgt
     6841 ggatgtacaa gcgcgtggtc tttggagcag tggtgcaccc ggcggtcgaa ggactggacg
     6901 atgtcaatgg ccgggaaatt tttgtcctcg cagttctggc cctggcagta ctctggatgg
     6961 ggctgtatcc gcttcccata gtggaagtca tgcatacgac ggtggacgat ttgttggctc
     7021 acctggcccg tagcaagctg tagcgggaac aagaagagaa cctgttcgag gatgatatga
     7081 attttctgca gcctgatttg gcgccggcct acccggaaat attcctgctg ctgatggttt
     7141 gcgtggtgct gttggccgat cttgcgtggg gcaaagagaa gccccatctc gcctatctgc
     7201 tggcgcaact tgcgctgttc ggttgcgtgc tgatcacctt tgcgacttct gcgccgggtg
     7261 tcgcctacac gttttcagga atgttcgtcg acgacccgtt ggccgacata ctgaagatga
     7321 tggtgtatgt gaccgtatct gtcgtgctgg tttactcgcg tacctatata tcggcgcgag
     7381 gactgctcag cggcgagttc ttcagcctgt cgctgtttgc cacgctgggc atgatggtaa
     7441 tgatctccgc gagtcatttt ctcacgctct acctcgggct cgagcttctc tccctttcac
     7501 tctacgccat ggtagcgcta cggcgagaat ccgttggggc gaccgaggca gccatcaagt
     7561 tttttgtgct gggcgcgctt gcctccggat tcctgcttta cggcatgtcg atggtatacg
     7621 gatcgacagg ttcgctgaat attgcgcgtg tggctgaagt gatccaatcg ggaacggtga
     7681 gccacacagt cctagttgtg ggcgtggtat tcatcgtcgc gggcatcagc ttcaagctca
     7741 gcgccgcgcc ttttcacatg tgggcccctg atgtctacga gggaggctcg accgctgccg
     7801 tgctgtttat cggatctgcg cccaagttgg ctgcattcgg ctttatcatg cggttgctgg
     7861 ccgagggcct gggtccaatg gtggctgact ggcaagggat gctcatcatc cttgcggtca
     7921 tgtccatggc gatcggcaat atagccgcca ttgcacagac gaatatcaag cggatgctcg
     7981 cctattccac gatctcgcac atgggcttcg tgctgctcgg cgttcttgcc ggcgatggaa
     8041 atggctacag cgcggcgatg ttttacgtgg tcgtctatgt cctgatgacg ctgggcacgt
     8101 ttggcgtgat catgcttctg tcacgaacgg gcttcgaagc cgagatgctg gacgacttca
     8161 aggggctgaa ccggcgcaac ccatggtacg cattcatgac cctcctgctg atgttctcga
     8221 tggctggaat tcctccgacc gtcggcattt atgcaaagct ctccgtgttg caggctgtgc
     8281 taagcgctgg ctatacgtgg cttgctatcc tcgccgtgct cctgtcgctc atcggggtgt
     8341 tttactatct acggatcgtc aagctgatgt atttcgatga gccggaaagc gatgccgcca
     8401 tctctcccca aggcgatgtc aaattgctgc tgagcgcgaa cggcctagcg atgctcgcct
     8461 tcgggttatt ccctcaagcc ttgatggcgt ggtgcgccta cgcgattcag caatcatcca
     8521 tatagcactg ggaatcacga gggcttcccc ctgggcctga tgctcgcttg agctgtttgt
     8581 gccggctgct ccatatatac aggcgcctgc gcttgcgggc tttttggaaa catcccgtaa
     8641 ggatgccgca ttcgatgccc atgagggcgc ttattttctg tagctgatgc ggtatatcac
     8701 tccggcataa tcatccgaaa ccagcagggc tccgtcgggc ataaccagga catccaccgg
     8761 gcgtccccac gcgttttcct gctccagcca tccttcggca aatacctctt gattcactac
     8821 cttgttatct tttatccgcg cgaactggag gcgatacccg cttttgtttc gccgattcca
     8881 tgagccatgc tccgctataa agatgttgtt ccggtattcg ggggggaaca ttttccccgt
     8941 atagaagcgc attcccaggg gcgctacatg cggatccagc ttggttacgg gtggcgtcga
     9001 tctgctgcaa tcgcgttttg ccccatactt cggatcaaga atatcgacag catggcaata
     9061 aggatagcca aaatgcatcc ccttgcgtgg ggcgcggttc agctcgtctg gaggcaggtt
     9121 gtcgcccatc cagtcccttc cgttgtcggt gaaccataat tcctttgttt ggggatgcca
     9181 gtcgaaaccc acggtgtttc gaaccccttg tgcgaagacc tcgtgcccgg taccgtcggg
     9241 cttcatgcgc gaaatcagcg caaacttctc ggggtcgggc tcgcagatgt tgcaaggcgc
     9301 tcctaccggc acatacagca gcccgtcagg gccgaaagca ataaattttc cgccatgatg
     9361 cttctcagtt ggaaagccat caaaaataac agttggcttg cctggttcgt ccagcttgcc
     9421 ttcaatccca tcaaatttca gaatgcggct tatcgcggag acatacagcg caccgtcctt
     9481 gaacgcgacg ccaatgggca tgctaagccc gctcgctatt gtttttacct tccgtccgcc
     9541 ctctgtttcg gttatggcat atacatttcc ctcggaggat gaaccggcaa acaccgtccc
     9601 attttgtccc agtgcca
//
LOCUS       DASSHJ010000264         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_849649, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000264 DASSHJ010000000
VERSION     DASSHJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849649"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFI43_10215"
     CDS             <1..253
                     /locus_tag="VFI43_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7062539.1"
                     /translation="VNAVINLAHVLDLTVVAEGVETDGQQQVLRGLGCDELQGFLVAK
                     PMPANTLSIWMSRREMIEPGELPSELSSADLKQAAFGAL"
     gene            complement(436..1119)
                     /locus_tag="VFI43_10220"
     CDS             complement(436..1119)
                     /locus_tag="VFI43_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET7062540.1"
                     /translation="MLYLILIGLIFFLVVGPSYWVKHTMEKYSEPTDRYSFTGAQLAR
                     KLLDDAKLPHVGVEVTESGDHYDPLKKMVRLTSDKFNGRSLTAITIAAHEVGHAIQDH
                     DNYPPLALRTTLVQWAAPAEKLGASILMLAPVITAAVRSPAAGLLFLAGGMLTLGSAT
                     LVHALTLPMELNASFARALPILERERYLIDGDMPHARRLLNAAAWTYVAASLMTLLNV
                     ARWWAILRR"
     gene            1602..>2804
                     /locus_tag="VFI43_10225"
     CDS             1602..>2804
                     /locus_tag="VFI43_10225"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET7062541.1"
                     /translation="MKNIYTEEGSRPIKAWADIEEDALAQARNLARLPFVDRHGIALM
                     PDCHLGRGTTVGTVIATDKAIIPAAVGVDIGCGMNAVRLSLKAPDLPDSLLAIRRQIE
                     RDVPLGPGGSRDKPVALGKLSSALEEGAGFKRWYERFAQGRKTDEISLLKKAQSQLGT
                     LGSGNHFIELCTDENQDVWVMLHSGSRGIGNMIGGYFIEKAKRRMERFSIHLPDADLA
                     YLTEGDDDFDDYVEAVHWAQDYALENRRLMMETTIAALQRHIPIKFTITREAINCHHN
                     YVERETHFGRNLWVTRKGAIRAGVGDLGIIPGSMGQRSYIVRGKGELESYCSCSHGAG
                     RKMSRTQARSTFTVADLIEQTKGVECRKDKMVLDEIPSSYKNLDEVMANQADLVEVVH
                     TLKAVMCVK"
BASE COUNT          704 a          707 c          769 g          624 t
ORIGIN      
        1 ggttaatgct gtgattaacc ttgcccacgt gctggatctg accgtcgtcg cggagggtgt
       61 cgaaaccgac ggtcagcagc aggtacttcg gggtcttggg tgcgatgaac tgcagggctt
      121 tcttgtcgcc aagcccatgc ccgccaatac gctatctata tggatgagca gacgggagat
      181 gattgagccc ggggaactcc cctccgaact ctcttctgcc gacctgaaac aggccgcctt
      241 cggagctctt taacaagtcc ccgtgaaact cgttgcccat agattgggga gattatcgga
      301 caataacaga tgcatgatgg aagctatggc tgcgtcgcga taatgattcc gagggccaat
      361 acatggacgc cgttcgtgtt ccagttttta ctggccactg gatgtgatca ggcatcactg
      421 aagccaaccg ttccatcatc ggcgcaggat cgcccaccag cgcgccacgt tgaggagtgt
      481 catcaaggaa gctgcaacat aagtccatgc cgccgcattg agcaaccggc gcgcgtgagg
      541 catatcccca tcgatcaggt agcgctctcg ctctaaaatc ggcagggcac gggcgaaact
      601 ggcgttcaac tccataggca aggtcagcgc atgcacaagc gtcgcacttc ccagtgtcag
      661 catgccaccc gcgagaaaaa gcagtcctgc cgccggagag cgtactgctg cggtaatcac
      721 gggagccagc atcagaatgc tagctccgag tttctcggcg ggcgccgccc attgaaccag
      781 cgtcgtgcgc aaggcgaggg gtggataatt atcgtgatcc tggattgcat gtccgacctc
      841 atgcgctgca atcgttatag ctgtcaatga acggccgttg aatttatcgg acgtgaggcg
      901 gaccattttt ttcaaaggat cataatggtc gcccgattca gttacctcca cccctacatg
      961 cggcaatttc gcatcatcga gcaattttcg ggcgagttgc gcgcccgtga atgaatagcg
     1021 atctgtaggt tcgctatatt tttccatcgt gtgcttgacc cagtaggaag gtccgacaac
     1081 caggaaaaag attaaaccga tcaatatcag ataaagcatg attgaaacta tcagtaaaaa
     1141 ttacttgatg agaccgctta aatgtcctga atgtttgctt atcggcaact tcagtcgatc
     1201 acgttgtatc agagctttga tcgataatgc tcaaaataat tcttcaactg aatcagaagc
     1261 aaatctctcg gagaggtaat gacgccccgg ggctaagaat cagttctgtc ttggcattgc
     1321 agtttaaaag tttataaaag cgaacttcaa agtcatcgtg tggtcaagcc gcctcgggga
     1381 taacttcgac cagaacgtaa ttcttcagag tttaatcctc tagagcgaag agaagctacc
     1441 ggtagttgct ccgataaacg acgtgtaagg gtatgtttga gggtaggcga atctgtggca
     1501 tacaacgctc caataatatt ccattcgaga gagtatctag ctcagaaacg cgacctatcc
     1561 ccaacaatac actgttcgct tgttcactta agcatccgcc aatgaagaat atctataccg
     1621 aggaaggatc cagaccgatc aaggcttggg ccgatattga ggaagatgcg cttgcacaag
     1681 ccaggaatct cgcgcggctg cctttcgtag accgccatgg tattgccctt atgcccgact
     1741 gtcatctcgg tcggggcacg acggtaggca cagtaatcgc gactgataag gcaatcattc
     1801 cagcagcagt cggtgtcgat ataggctgcg ggatgaatgc tgtccgcctt tcgctcaaag
     1861 ctccggatct gcccgattca ttgcttgcta tccgccggca aatcgagcgt gatgttccgc
     1921 tgggaccggg tggaagtcgc gacaaacctg ttgcgctagg caagctctcc tcagcacttg
     1981 aagaaggggc gggcttcaag cgatggtatg agcgttttgc gcagggccgc aagaccgatg
     2041 aaatcagcct tctgaagaag gcccaatctc agctgggtac cttaggctca ggaaaccact
     2101 tcattgagtt gtgcacggat gaaaatcagg acgtgtgggt gatgcttcac tcgggttccc
     2161 gcgggattgg caacatgatc gggggttact tcattgaaaa agccaagcgc aggatggaac
     2221 ggttttccat tcatttgccg gatgccgatc ttgcctatct gacggaaggc gatgacgatt
     2281 tcgacgatta tgtggaagct gtccactggg cgcaggacta tgcgcttgag aaccggcggc
     2341 tcatgatgga gacgacaata gcggcgctcc aacggcatat tccgatcaaa tttacaatca
     2401 cgcgggaagc tatcaactgt caccacaatt acgtggaaag ggagacccat ttcgggcgaa
     2461 atctctgggt gacacgcaag ggagcaatac gggcaggcgt gggcgacctg gggattatcc
     2521 ccggctcgat gggccagcgc agctatattg tacggggcaa aggcgaactg gaatcctact
     2581 gctcctgctc gcacggtgct ggccggaaaa tgtcacggac gcaggcacga agcaccttta
     2641 ccgtggcaga cctgatagag cagaccaaag gagtcgagtg ccgcaaggat aaaatggtgc
     2701 tggacgagat accgtcatcg tacaagaacc tggatgaggt catggcaaac caggcagacc
     2761 tggttgaagt cgttcacaca ttaaaagcag tcatgtgcgt caaa
//
LOCUS       DASSHJ010000265         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1145355,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000265 DASSHJ010000000
VERSION     DASSHJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145355"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..522
                     /locus_tag="VFI43_10230"
     CDS             328..522
                     /locus_tag="VFI43_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062542.1"
                     /translation="MMKRHNERYLLDFAFALLDHRSGAFRQKLLKYFQSTGRKLWTDV
                     EKIPGRKRNDPVSPFQMMSD"
     gene            700..1467
                     /gene="ygiD"
                     /locus_tag="VFI43_10235"
     CDS             700..1467
                     /gene="ygiD"
                     /locus_tag="VFI43_10235"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HET7062543.1"
                     /translation="MPALFVGHGNPMNALSQNAYTSAWSAIGSALPETRAIVCISAHW
                     YIPKTAVTAMRNPPTIHDFGGFPRELYQVQYPAPGSPEVAAEVMELLSPFALSDMEWG
                     LDHGAWSVLLHIFPNADVPVVQLSIDETRDAAWHYDIAKRLQPLREKGILIVGSGNIV
                     HNLHTYSWGKHDAGPYDWAIRFEDRVKEALRSGDAGSLVEYQALGRDALLSAPTPDHY
                     LPFLYILAQRRGDESITFPVEGFDGGSISMLAVKIGQ"
     gene            complement(1600..1836)
                     /locus_tag="VFI43_10240"
     CDS             complement(1600..1836)
                     /locus_tag="VFI43_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062544.1"
                     /translation="MQVDKWAGISSAWRMTVLVVLLLNAGAGLADEWKPAAPIPKGAE
                     EVYGIAPGGKLHVFGGLGLDWKAMGMVMEYDPRA"
     gene            2410..3411
                     /locus_tag="VFI43_10245"
     CDS             2410..3411
                     /locus_tag="VFI43_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7062545.1"
                     /translation="MKYLGHLSRDDPFYGYLRYDIFPLLNVSLDKSPDFRVYRLPASN
                     HVYAYEERNSHARIIGKFFGGVSRLSSGGARNRMEREYSNLNYLRGIGFAGYPHYVPR
                     PLGRNADLNNVLVEEYCWGAPLADFIINAIHQNERDALFQKLGALAYFLATLHNRTAS
                     DSKVDFNKDCKYFDLILQHLEGNGRLGADEIREFHRLREYWQGKSGMWEDRQVVAHND
                     VTPANILFGGCPWVIVIDLERMRLGDRVLDLGRVVGELQYYFLRYAGNRWLAEPFIGH
                     FLWEYVCHFPDRDAAFRSITGRIPFYAGVTLLRIARNLWLAKHYSRQLIDEAKKMLR"
     gene            3417..4136
                     /locus_tag="VFI43_10250"
     CDS             3417..4136
                     /locus_tag="VFI43_10250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7062546.1"
                     /translation="MIKGILFDLYGTLIDIETNEGMEEIYRAIAHYLTYHCIYLRRWE
                     VRDRYYRIMKQQKEARGEEYPEIDVEAIWNEFLMQEGIRSPSIRGQQAKVIAQIYRGI
                     SRNRLQLYPGVKEVLNELRTRYQLALVSDAQPCYALPEIRAVGLDGYFDPIIISSYYG
                     YRKPDSRLFRTALERLGLEPHEVISIGNDMFRDIYGAKILGIKSIFFDSNQGTKTFQD
                     VSPDYQISQFDDVLKGVAALG"
     gene            complement(4476..5048)
                     /locus_tag="VFI43_10255"
     CDS             complement(4476..5048)
                     /locus_tag="VFI43_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7062547.1"
                     /translation="MWTNPANQHLIAASSQGLVDINPVAGTFRVIKSGLFPDGVSVSP
                     DGKMAYVENGVIQAYDISTGSLVRSFPNFGHGPDGTGVIIGGPLNGDIIVNNNDGTVG
                     LLDPAKPSGDPDQFVLIPTGGTRGDFVSPDHNSGTLFLSQNEQVARLSCGPDCSIGAP
                     IPEPESYALMLAGLALLGWTVRRKNSLREG"
BASE COUNT         1260 a         1490 c         1371 g         1316 t
ORIGIN      
        1 tgcaaacaat cgttctgtac ggtttcgcac aaacaaccgc cgtgcgcctg ctcacaatac
       61 aatcataagt ccaaagaaaa gcgttttttc acattcctgc aaaggttctg atttaaggtg
      121 agggctggca acagtattgt aaaacaaccg gatttgacgg ggatatcgct attcttaggg
      181 ctctgacaaa gtccgccatg gggtaattcg atagagaggg agaacttgtt cagagagatt
      241 cgcgtttccg tcgcgtcata cgcatgtttc gtcgattgcg ttatggtttg ttcagcattt
      301 tggttttcac ccctttaaca ggaatagatg atgaaaagac acaatgaacg atatctgctc
      361 gactttgctt ttgcactgct cgatcaccgg tcgggagcgt tcagacaaaa attgctcaag
      421 tattttcaat ccaccgggcg aaagctctgg acggatgtag agaagatacc gggacggaaa
      481 agaaacgatc cggtgagccc gttccagatg atgtccgact gacagataag ccaatacgca
      541 ccctcccaat ccaggaacgg accaagctgc gctgtcggcc cgttcgcgcg ccaaaaacac
      601 gcccgtacct cgggtttcct cccttgactc ctttgaatgt tgcatcggat gatggctgag
      661 acagaaaaca tcggttcttc ctctccactt ccccgcgtta tgcccgcgct gtttgtcggc
      721 catggaaacc ccatgaacgc cctctcgcag aacgcctata cctcggcctg gtctgccatc
      781 ggctccgccc ttcccgaaac cagggccatc gtgtgtatct cggcgcattg gtacattcca
      841 aaaacagcgg ttaccgcgat gcggaacccg cctacgattc atgacttcgg cggctttccg
      901 cgggaacttt accaagttca gtatcctgcc cctggttcac cggaagtggc ggcagaagtg
      961 atggaactac tgtcaccgtt cgccttgagc gacatggaat gggggcttga ccatggcgcc
     1021 tggtctgtcc tcctgcatat ttttcccaat gccgatgttc ccgtggttca gttgagcatc
     1081 gacgaaacgc gagacgcggc ctggcactat gatatcgcca aacggttgca gccattacgg
     1141 gagaagggaa ttctgatcgt gggaagtgga aatatcgtgc acaacctcca tacctattcc
     1201 tggggaaagc atgacgcggg gccgtacgac tgggctataa gattcgaaga tcgcgtaaaa
     1261 gaggcattaa ggtcgggaga cgctggatcg ctcgtcgaat atcaggcgct tggccgggac
     1321 gctttattat cagcgcccac gcccgaccat tacctaccct ttctctacat tctggctcag
     1381 cgcagagggg atgaaagcat cacgtttccc gtggaaggat tcgacggtgg ctcgatctcg
     1441 atgcttgccg tcaaaatcgg gcaatagacg tcattgggga tatttaaaga cgcgttattc
     1501 cctttcgata cttcccttaa acgcctttcc aagtgtatcg ccctgtctgg ctgcttgcgc
     1561 aaatcgttgg gtcatgctct gcgaatcttg caggtcccgc tacgctcgcg ggtcatattc
     1621 cattaccata cccattgcct tccagtccaa tccaaggcca ccaaagacat ggagcttgcc
     1681 ccccggcgcg attccatata cttcctccgc ccccttcggg atgggcgcag caggcttcca
     1741 ttcgtcggcc aaccctgcac ccgcgttaag gagcagcacg acaagtactg tcatgcgcca
     1801 ggcgctcgaa atccctgccc atttgtcaac ttgcatttat gtctctcctt ttcccgggaa
     1861 aaaccctgcg ttccctgctc caggcataat agtcatcgta gttcatcgac cactacacgc
     1921 tttccatcgc tccgaggagg agtatcgagc tttatccgct ggttgcacaa ggtgttctcg
     1981 aaagtcctgc gctggtggca tccaaaacag gtggccgaaa caggtttcac ttttccgagg
     2041 tacgctaaaa aagcgggaac gggacgtcga ggtccgcagc acttttccct tggtccctat
     2101 cttccggcct gttccaacta ctctggctcg tcgtgtctcc caaataccgg atatgcatac
     2161 cagggcctgt tagctgtcca ttaattttct caaacggcgg gtagcccggc taagcggtgt
     2221 atcatcattc gtcattggtt ggcttcgact cttacaacac ctcgcaccta ctttatattg
     2281 tgggctaatg gaattcatgg catataaaac gaggattccc ggaatgtctg ttgccttccg
     2341 gaaggccaaa gcgaccgtcc gcttcacgac tattcaccct tctcccctgc gccctccttt
     2401 acagcgacga tgaaatacct gggacatctt tcgcgcgacg atccttttta tgggtattta
     2461 cggtatgaca tttttcccct tcttaatgtc agcctggata aatctcccga cttccgggtg
     2521 tatcggcttc cggcttcgaa ccatgtctac gcttatgagg aacgcaatag ccatgcccgg
     2581 attatcggca agttttttgg gggtgtttcg cgtctgagtt ccggcggtgc gcgaaaccgc
     2641 atggagcgtg aatacagcaa cctcaactat ctgcgcggca ttggctttgc tggctacccc
     2701 cattatgttc cccggccgct tggccgcaat gctgatctca ataatgtgtt ggtagaagaa
     2761 tattgctggg gggcgccact tgccgacttc attatcaacg cgatccatca gaatgagcgg
     2821 gatgcattat tccaaaaact gggcgcactc gcctattttc tcgctacgct ccacaaccgc
     2881 accgccagcg atagcaaggt agatttcaat aaggactgca aatattttga tcttattttg
     2941 cagcacctcg agggaaacgg gcgtctgggc gcggacgaaa tacgcgaatt tcaccgtttg
     3001 agggaatact ggcaggggaa gtcaggcatg tgggaagatc gccaggtcgt ggcgcacaat
     3061 gacgtgacac ccgccaacat cctgttcggt ggttgcccct gggtcatcgt gatcgatctc
     3121 gaacggatga ggctcgggga tcgagttctc gaccttggaa gggttgtggg tgaacttcag
     3181 tattactttt tgaggtacgc tggcaacaga tggttggccg agcctttcat cggccatttc
     3241 ctatgggagt atgtgtgtca ttttcctgac cgcgacgccg cattccggtc aattaccggg
     3301 cgcattccgt tctatgctgg agtcacgctc ctgcgcattg cacgaaactt gtggctggcg
     3361 aagcactaca gccggcaact gatcgacgaa gcaaaaaaaa tgttgaggta acgatcatga
     3421 taaaaggcat tttgttcgac ctctatggca ccctgatcga catcgaaacc aacgaaggca
     3481 tggaagaaat ctaccgtgcc attgctcact accttactta tcactgtatt tatttgcgcc
     3541 gctgggaagt ccgcgaccgg tattatcgaa tcatgaagca gcagaaagaa gcgcgcgggg
     3601 aggaatatcc tgagatcgac gtcgaagcga tctggaatga gtttctcatg caggaaggca
     3661 tccgatcacc ctcgatccgg gggcaacaag ccaaagtcat cgcccaaatt tataggggaa
     3721 tctcgcgtaa tcggctgcag ctttatcccg gcgttaaaga agtgcttaat gaattgcgaa
     3781 ctcgctacca actggcgctc gtttcggacg cacagccctg ctatgccctg cccgaaattc
     3841 gtgccgtcgg actggacggt tacttcgatc cgatcatcat ctccagctac tacggctacc
     3901 ggaaaccaga ttcgcggctt ttcagaaccg cgctcgaaag gttgggcctc gaacctcatg
     3961 aagtaatcag cataggcaat gatatgttcc gggacatcta cggcgcgaag attctcggaa
     4021 tcaaaagcat ttttttcgat tccaatcagg gaaccaagac gtttcaggac gtttcgccgg
     4081 attaccagat ttcacagttc gatgacgtgc taaagggtgt ggcggcgcta ggatgattct
     4141 atgttaacga agtggtctag aaaaaatatg atgggacgaa gaattgggga cggagaagtt
     4201 gggaataacc cagcgtttcc ttatgcgcag tagtcgcggc ctctagcaag cccccagtcg
     4261 aaaaaaccta aagaaatccg gttctgcccc caattttaac ctcttggcgc attttccggt
     4321 taatccgttt tccgtttccc accattcaaa cgtactaccg ctggcctggc caacacattc
     4381 cagtccacct cccgacagtc gatgcggggc aacgttcctc ttgccacagg cggcgcggac
     4441 agccagctcg acgtgaaaaa aacctttata ctcgatcaac cttcgcgcag cgaattcttc
     4501 ctccgtaccg tccatcctag aagggccaaa ccggcaagca tcagggcata agactcaggt
     4561 tctggaatag gcgctccgat cgagcaatcc ggtccgcacg acagtcgcgc cacctgttcg
     4621 ttttgcgaaa gaaacagtgt accgctgtta tgatctggcg agacaaaatc accgcgtgtg
     4681 ccgccagtgg ggatgaggac gaactgatcc ggatcgccac tcggcttggc cgggtccaga
     4741 aggcccaccg tgccgtcatt attattgacg atgatgtcgc cgttcagcgg tccaccaatg
     4801 atgacgcccg tgccatcggg gccatgtcca aaattgggaa aacttcggac cagggaaccc
     4861 gtcgagatat catatgcctg tataacccca ttctccacgt aagccatctt cccgtccggt
     4921 gatacagata cgccatccgg aaacaggcca gatttgatga ctctaaaggt gccagctacg
     4981 ggattaatgt cgaccaaccc ctggctgctt gctgcgatga ggtgctggtt ggcaggattg
     5041 gtccacattc ccaagtagcc gcgaaggatg ctcgtccggc ttcgaatacc atccgtaccg
     5101 caccctcacg cacttagggg gaaactttgt ttgatttatc atggttccat tctccaatgt
     5161 tggagccttc ttaaaacccg gggctgttga ctccggcatc ccttaatttc cctggcttga
     5221 tcttctgaag ctatctgcta aaccgaaggt gatacgtggt caactgaaga gggctaataa
     5281 ggcagcatga acaaaacgtt agaacgacag aagcagtttt cgaagccttt tagctttttc
     5341 gagttttaaa gggaggacga tgaacaacaa acctggctcg acccgactgc ttgccccgag
     5401 gcgccgtccc ttgcgggttt ttgccttcga cccgagc
//
LOCUS       DASSHJ010000266         8007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1036263,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000266 DASSHJ010000000
VERSION     DASSHJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036263"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFI43_10260"
     CDS             <1..870
                     /locus_tag="VFI43_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7062548.1"
                     /translation="EQALRHILQFRGPDPEITEAMIWLLLSGKKVDHAFLDAIVQPFR
                     PQSGGRPALSAHLTEALAAAEQTLGKPLQAAEWYQQSLAARPRDFLWTLTLADNMEWA
                     GCPSSANHVRFVALKLFASPNFKQIDVQYPLRLAEYFSGRKDMLAQGGTWVEPESEKW
                     LSLRRNWNLSRTLDNADYFSLQRQTERLSSPAWQKFAGYVGGKNQAAVALQLKAISTY
                     LEGQPDKKQKQASEILPLSLDDVDREQRWLAGKALASPSDLHAEADVCRQTLAKIRGS
                     QQVSSAGEEQPAL"
     gene            867..2147
                     /locus_tag="VFI43_10265"
     CDS             867..2147
                     /locus_tag="VFI43_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein PelD"
                     /protein_id="HET7062549.1"
                     /translation="MKKPWLEGMEIISQPMPAWGGWIETFGLTAIVLGVSFITQQNDP
                     FRLGVGFPWPVLAPLLAGLRYGFIHGFASALAILAALGISLDQQWQVASELPLSFAIG
                     VVIVAMVAGEFRDIWGRRLHRLEGAYQYRAERLEEFTRNYQLLRLSHDRLEQTVANSG
                     LSLREGIMHLQSSLDSIDGTSDLPGPSLQRLIEFMAGYASLTRACIVGIRGNRVRANV
                     LARVGEPFEIDESDPVLTGALETGELTAVNSMAGTSGGQRQLLVAIPLSDSTGEVHAM
                     LLVKSMPFFAFHENNLKLIAVLAAHGIDHLRFGTAASSVARFIAAFDRVYQDHLTFKL
                     DATLLRLSGNPPDVEAANGKLRSAIRAIDITCIACDNGKPVVWILLPLTDSVHAQAWM
                     QRLDSVLAKMTAELFAIGEVDPQRIRSLSPQKSS"
     gene            2147..3184
                     /locus_tag="VFI43_10270"
     CDS             2147..3184
                     /locus_tag="VFI43_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein PelE"
                     /protein_id="HET7062550.1"
                     /translation="MPGLVMLWGLVAEVLAGVLLWLSEGAGEEAWAGAPGVFGAIILH
                     TLSSYFFAQGFWRILPPRYKLPTSRSLGFLFSVIWILPVLGVLGILWSVTAALKRPRN
                     RSLKDVQTIALPELPFSPPFIFPTPPYSEGALRQIVHFAERPLKRLKAVMATRSIPAV
                     AAMPIWSKATRDPVDDVRLLAYAMKDDNEKSLTDRILVLAEALPGGGPHMQTVYHKTI
                     AALCWELLYHRLVQGALRQHWIQMARRHMEASLRVSSTEPDTAEIEDPDAWLLYGRIL
                     LESQELDKAKAAFGNARAGGIDEQKILPWLAEIAFEQGCYGQVKHYLSMLSRTCEKGK
                     ELALVKEWWNK"
     gene            3169..4764
                     /gene="pelF"
                     /locus_tag="VFI43_10275"
     CDS             3169..4764
                     /gene="pelF"
                     /locus_tag="VFI43_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HET7062551.1"
                     /translation="MVEQVKADFLEANLSAARSGERTVADIGLLLEGTYPYIRGGVSS
                     WVHQIISGLPDIRFAVVFIGGESGMYGPAQYQFPPNVTHVETHYLLQGQTNSKPGARR
                     GNARAFEKMEALHAYLRREGEMPGEQLERAFTGLGSPEGISEEDFLYSKASWEYITHQ
                     YRRRCTEPSFINYFWAVRAMHAPLFVLARIARELPPVRAIHSISTGYAGFLGAIIQLR
                     RKIPFILTEHGIYTKERKIDLAQATWIRDHNDDVCNTLHDEMGYIRGLWIRFYEQIGR
                     MAYAHASPIVSLYEGNRLRQISDGAEAEKTLVIPNGISVQRYCVALEKRVNEIPPVLG
                     LIGRVVPIKDIKTFIRTLRILANERPDVQGWIVGPEDEDPSYVNECKELVASLGLQNS
                     VKFLGFQNVLEILPQLGLLVLTSISEALPLVILEGFASGVPCLATDVGSCRELIEGRG
                     EEDRGLGAAGSVVFIADPEGTAKAARQLLDNPERWRAAQRAGLERVKRYYDDKLMFSS
                     YRNLYQQALDRDSEGFAELSGAT"
     gene            4767..6137
                     /gene="pelG"
                     /locus_tag="VFI43_10280"
     CDS             4767..6137
                     /gene="pelG"
                     /locus_tag="VFI43_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HET7062552.1"
                     /translation="MAGIGFEIRKILERDSYWSVLRAYGYAGVVSGGPWALSILSIMM
                     IGILAVMLGVAQREVNAFQICVTYLMAASLIWSGGMQLMFTRFVADRAYAGEQAEVLP
                     NLFGALIITMGGGFALFAPPIFWLFDGPFFQQLLLLTNFVVLSGLWIALIFLSGMKAY
                     RRIVWTLAKGYTIGIIVALAASAWALNGLLLGVLAGHGYLLFSFLHHIVREYPGNSLL
                     KLDFLDRKRSFYSLFAVGTLYYLAVWVDKFIFWFVPHTSEPVIGPLRASIIYDLPIFL
                     AYLFILPGMAVFLVSMEADFAEQHERFYKAIREGDTLMHIEYRRDRMVYAARQGIYEI
                     FKVQGITVALCLLWGRDLLHAIGISPLYIHLFYIDVVAVSVQVLLMAILNVLFYLDAR
                     REVLLITACFFITNLLFTFATLQLGAETFGYGFAASVSLSAFLGLFILSRKFNRLEYE
                     TFMLQG"
     gene            complement(6175..>8007)
                     /locus_tag="VFI43_10285"
     CDS             complement(6175..>8007)
                     /locus_tag="VFI43_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7062553.1"
                     /translation="QRLLGVASAIGSQFSPLLLGTVLDMDVAEVDRCCDALARRGQIL
                     GMDGMEQNPQGEVACTYIFRHALYVEALYQRLSASQIIRMHLRIGECLERLHGQNDLK
                     HAAELALHFEKGWGWERAVFYLTQAANNAARRFANREAYDYLARALAMVERLPEEKQA
                     ETRIALLRQTSAIRRSMGEMSDAKADLELMLVTARAAGDNRAEVLALLELSRVLVWLD
                     RRKCLETAEQALARSNHLEDKILQSIVKGMWGGLNLMLGSWRADCAEACDNAMDQARV
                     SGNPLVLHSRLTQHIYVEVLASNYRRASATADKALELSRSLGDGYMFMVGHYYQGLAL
                     LHLGEWGKLRRISEESRHAFESNCDDVTLPFRLHGQILMAWLHVEAGDFATAKIYCEE
                     ALQENRGPWERFISAHFSAIYGRALLGVRNHEGAIRCFDSFFKAEEDESLPVSRNYCF
                     PAYLGASETWLSLGDFEKARYYASRLIDLAQGAPEKTYLALGYRLLAETALRAGVFDQ
                     ASLEIANALAITENSEVPLAAWRVYSTAEQLHNLEGETTKAGAFLNKRQEAIRKLRDS
                     LPESDSLQECLHHRMGFEVPTVIDGGADATADQVSFNVRLVDAA"
BASE COUNT         1657 a         2183 c         2256 g         1911 t
ORIGIN      
        1 gaacaggccc tgcgccacat tctccagttt cgcggtcctg accctgaaat taccgaagcc
       61 atgatctggc tgttgttgtc gggcaaaaag gtggatcatg cttttcttga tgccattgtt
      121 cagcctttcc gccctcagtc agggggcagg ccggcactca gcgcacactt gactgaagca
      181 cttgcagcgg ctgaacaaac cttgggcaag cctcttcagg cggcggagtg gtatcagcaa
      241 tcgctggcag ccaggcccag ggatttttta tggacgttga cgctggccga caatatggag
      301 tgggcgggat gcccgagcag tgcaaaccat gtccgtttcg ttgcgctaaa gctctttgcc
      361 tcgcccaatt tcaagcagat cgacgtgcaa tatcccctgc ggctcgccga atatttctct
      421 gggcgcaaag atatgcttgc ccagggggga acctgggttg agccagaaag tgagaaatgg
      481 ctgtccttac gccggaactg gaacctttcc agaaccctgg acaatgcgga ttatttttcg
      541 ctccagcgtc aaaccgaacg gttgtcctcg cctgcctggc aaaaatttgc cggatacgtc
      601 ggcggcaaga atcaggcagc ggtcgcattg cagttaaaag cgatttccac ttacctggaa
      661 ggccagcctg acaagaagca gaagcaggca tccgaaatat tgcccttgtc acttgacgat
      721 gtcgatcgcg aacagcgatg gctggctgga aaagctttgg cgagtcccag cgatttgcat
      781 gctgaagcag atgtctgccg ccagacgctc gctaaaatcc gcggctcaca gcaagtatct
      841 tccgcaggcg aagagcaacc cgcgttatga agaagccgtg gctggaaggt atggagataa
      901 tcagccagcc tatgccggca tggggaggct ggattgaaac atttggattg acggcaattg
      961 tgctgggcgt gtcgttcatt acccagcaga atgatccttt ccggctcggc gtgggttttc
     1021 cctggccggt actggctcct ctgctggccg gattacgcta cggtttcatt cacggtttcg
     1081 ccagcgctct ggcgatactg gcggcactgg gaatatccct cgatcagcag tggcaggtag
     1141 cgtccgaact tccgctttca tttgcgattg gggtcgtcat tgtcgcaatg gtggcgggcg
     1201 agtttcgtga tatctggggg cgccgtctgc atcggttgga gggcgcttac caatatcggg
     1261 ccgaacgcct cgaagagttt acccgcaatt accagttgtt gcgcctctcg catgatcgcc
     1321 tggagcaaac tgtagccaac agcgggcttt cgctccgcga gggcatcatg catctgcaaa
     1381 gctcgcttga ttccattgac gggaccagtg atcttcccgg gccttcattg caaagattaa
     1441 tcgagttcat ggctggatat gcctccctga cgagagcttg cattgtcggt atacgcggga
     1501 atcgcgtccg tgccaacgtt cttgcccgtg ttggcgagcc ctttgaaatc gatgaaagcg
     1561 atccagtact gaccggagcg ctggagacgg gagaattgac agcagtcaat tcgatggcgg
     1621 gaacttcagg aggtcaacgc caattgctcg tggccatccc cttgtccgac tcgaccggcg
     1681 aagttcatgc catgctactc gtgaaatcca tgcctttttt tgcatttcat gaaaataatt
     1741 taaagctcat tgctgtacta gctgctcatg gtatcgacca tttgcgtttt gggacagccg
     1801 cttcttccgt tgcgcgtttt atcgccgcat ttgaccgcgt ttaccaggat cacctcacgt
     1861 tcaagctgga tgcaacgctc ctgagactat cgggtaaccc gcctgatgtg gaggcagcaa
     1921 atggaaaact gaggtctgct atccgggcta tcgatattac ctgcatcgcc tgtgacaacg
     1981 gcaagcccgt cgtgtggatc cttctgccgc tcaccgattc tgttcatgca caggcgtgga
     2041 tgcagcggtt ggacagcgtt cttgcaaaga tgaccgccga gctctttgca atcggggagg
     2101 tggaccctca gcgcattcgt tccctcagtc ctcaaaagtc cagctgatgc ctggcctggt
     2161 catgctatgg ggacttgtgg ccgaagtgct cgccggggtg ctgctctggc tcagcgaggg
     2221 ggcgggggag gaggcttggg ccggagcgcc gggcgttttt ggcgcgatta ttttgcacac
     2281 cctctcatcc tatttttttg cgcagggttt ctggcgcatc ctcccgccaa gatacaagct
     2341 gcctacgtcg cgcagcctgg gttttctgtt ttcagtcata tggattttgc cggtactcgg
     2401 cgttctgggg atactgtgga gtgtgacagc tgcgctaaaa cggcctcgca accgttcgtt
     2461 gaaagatgtg cagactatcg ccttgcctga gttgcctttt tccccgccgt tcatttttcc
     2521 gactcctcca tacagcgaag gtgctctgcg ccaaattgtg catttcgccg aaaggccatt
     2581 gaagcgtctg aaggctgtta tggccacacg ctccataccg gcggtcgcag ctatgccgat
     2641 atggtccaaa gccacccgcg accctgttga cgatgtacgg ctattggcct acgccatgaa
     2701 agacgacaac gaaaaatccc tgacggatcg catcctggtt ttggctgagg cattgcctgg
     2761 gggaggcccg cacatgcaaa ccgtttatca taaaaccata gcggcgttat gctgggaact
     2821 gctgtatcac cggctggtac agggcgcgct caggcaacac tggattcaga tggcccggcg
     2881 ccacatggag gcctctctcc gggtctccag cactgagcca gatacggcgg aaattgagga
     2941 ccccgacgcg tggctgctgt acggacgcat attgctggag tcgcaagaac tggacaaggc
     3001 gaaagcggct tttggcaatg cgcgcgcggg cggcattgat gaacagaaaa ttcttccctg
     3061 gctcgcggaa atcgccttcg agcaaggctg ctacggtcag gtgaagcact atttgtccat
     3121 gcttagccga acctgtgaaa aaggcaaaga acttgcattg gtaaaagaat ggtggaacaa
     3181 gtaaaagctg attttcttga agcgaacctg tctgcagccc gatccgggga aagaacggtt
     3241 gccgatatcg gcctgttgct ggagggcaca tatccttaca tccggggggg cgtctcgtcc
     3301 tgggtgcacc agattatcag cggtcttccc gatatccggt tcgctgtggt tttcatcggg
     3361 ggcgagtccg gaatgtatgg cccggcgcag taccagtttc cgcctaacgt cacccacgtg
     3421 gaaacacatt acctgctgca agggcaaacg aatagcaagc cgggcgcacg aaggggaaac
     3481 gccagagctt ttgagaagat ggaagcgctg catgcttact tgcgacgcga aggtgaaatg
     3541 ccgggtgagc aattggagcg tgcgttcaca ggcctgggtt ccccggaggg catcagcgag
     3601 gaagattttc tgtacagcaa ggcgtcgtgg gaatacatca cccatcagta ccggcggcgt
     3661 tgtaccgagc cgtcattcat caattatttc tgggcagttc gcgcgatgca cgcccccttg
     3721 ttcgtacttg cgcgcattgc ccgggagctt ccgcccgttc gagcaatcca ttccatttcg
     3781 acgggctacg cgggctttct gggcgcgatc atccagttgc ggcgcaagat tccgttcatt
     3841 cttacagaac atggcatcta cacgaaggaa cggaagatag atttggcgca ggcgacctgg
     3901 attcgcgacc ataatgatga cgtctgcaat acgcttcacg atgagatggg gtacatccgg
     3961 gggctctgga tcaggtttta cgagcagatc ggccgcatgg cttacgccca cgcatcgcct
     4021 attgtcagcc tgtacgaagg aaatcgcctg agacagatat ctgacggggc tgaggcggaa
     4081 aaaacactcg tcatcccgaa cggaatcagc gtgcagcgtt attgcgttgc gcttgaaaaa
     4141 cgggtaaatg aaattcctcc tgtccttgga ctgattggac gcgttgtgcc catcaaggac
     4201 atcaagacct ttatccggac attgcgcatc ctggcgaatg agcggcctga tgttcaggga
     4261 tggattgtcg gtcccgagga tgaagatcca tcttacgtga acgaatgcaa ggagcttgtt
     4321 gccagcctgg gcttgcagaa ttccgtcaag ttcctggggt ttcagaacgt gcttgaaatt
     4381 ttgcctcaac tgggtctgct tgtattgacc tcgatcagcg aagctctgcc gctggtcata
     4441 ctggagggtt ttgccagcgg tgtgccctgc cttgcgacgg acgtgggctc ttgccgcgaa
     4501 ctcattgaag ggcgaggaga ggaagatcgc gggctcggtg cggcgggaag cgttgttttc
     4561 atcgcggatc ccgagggaac ggcaaaggcc gcgcgccaac tgcttgataa tccggagcgc
     4621 tggcgcgccg ctcagcgtgc gggactggag cgggtaaagc gctactacga tgacaagctg
     4681 atgttctcat cctatcgcaa tctttatcag caggcgctgg atcgggattc cgaaggcttt
     4741 gcggaattat ccggggcgac ctgaagatgg ccggcatcgg gtttgaaatt cgcaagatcc
     4801 ttgagcggga cagttattgg tcggtcctgc gcgcttacgg ctacgctggg gtagtcagcg
     4861 gaggtccttg ggcgctttcg atcctcagca tcatgatgat cggcattctc gccgtgatgc
     4921 tcggcgtggc tcaacgcgag gtcaacgcct tccagatttg cgtgacctac ctcatggcgg
     4981 cttcgctgat ctggagcggc ggcatgcagc tcatgttcac gcgtttcgta gccgacaggg
     5041 cttacgcggg ggaacaggca gaggttcttc ccaatctctt cggcgcattg atcatcacca
     5101 tgggaggcgg cttcgcgttg ttcgcgccgc ccattttctg gctatttgac gggccgtttt
     5161 tccagcagct attgctgctg accaattttg tcgtcttgag cggactatgg atcgccctga
     5221 tctttctatc gggaatgaaa gcctatcgcc gcatcgtgtg gacgcttgcc aaaggctaca
     5281 cgatcggcat tattgttgca ctcgcggcat ccgcctgggc gctgaatggc ctgttgctcg
     5341 gcgtattagc ggggcacggt tacttgctgt tttcgttttt gcatcacatc gtgcgcgagt
     5401 atcctggaaa ttccctgctc aagcttgatt ttctcgaccg gaaacggagc ttctacagcc
     5461 tattcgccgt tggaaccctg tattaccttg ccgtctgggt agacaagttc attttctggt
     5521 ttgtgccaca cacttccgag ccagtgatcg gcccattgcg ggcctccatt atttacgacc
     5581 ttcccatttt cctcgcctat cttttcatac tgccaggcat ggccgtattc ctggtcagca
     5641 tggaagccga tttcgccgag cagcacgagc ggttttacaa ggccatccgg gagggagata
     5701 cgctcatgca tatagagtac cggcgggatc ggatggttta tgctgcacgg cagggaattt
     5761 atgagatttt caaggtccag ggcataacgg ttgcactatg cctgctatgg ggcagggatc
     5821 tgttgcatgc aattggaata tctccgctat atatccacct gttctatatt gacgtggttg
     5881 cggtgagcgt ccaggtgttg ctgatggcaa tactcaatgt gcttttttac cttgatgccc
     5941 gccgcgaggt gctgctcata acggcatgct tttttatcac caacctgcta tttaccttcg
     6001 cgacattgca attgggcgcg gagactttcg ggtacggctt cgccgcttcg gtatcgctct
     6061 cggcttttct cggactattt attctgtcga gaaaattcaa ccgtcttgag tatgaaacct
     6121 tcatgctgca gggctaggct cccgcagcag aaatcatctg aggcggggca gggatcacgc
     6181 cgcatcgacg agtcgcacgt taaagctgac ttgatctgca gttgcatccg ctcccccatc
     6241 aattaccgtt ggaacctcga atcccatccg gtgatgcaaa cactcctgta atgagtcgga
     6301 ttcaggtagt gagtcccgca gtttcctgat cgcttcctgt cgtttgttca ggaacgcccc
     6361 cgccttggtg gtttcacctt cgagattgtg aagttgctca gccgtgctat agaccctcca
     6421 tgcagcaaga ggcacttcag agttttccgt aatggcaagc gcgtttgcaa tctctaaaga
     6481 ggcttggtcg aagacgcctg ccctgagggc ggtttcggcc agcaggcgat aaccgagcgc
     6541 aagatatgtt ttctcgggtg ctccctgtgc cagatcgatc aaccggctgg cgtaatagcg
     6601 cgccttttcg aagtctccca gcgacaacca ggtttcgctg gcgcccagat atgcgggaaa
     6661 acagtaattt cgggagacgg gcagcgattc gtcttcttct gctttgaaaa aggaatcgaa
     6721 gcaccggatt gcgccttcat ggttgcgaac cccaagcaga gccctgccat atatggctga
     6781 aaaatgcgcg gagataaaac gttcccacgg accgcggttt tcttggagcg cttcttcgca
     6841 atatattttc gcggttgcaa aatcgccggc ctccacgtgc agccaagcca taaggatctg
     6901 tccatgcagg cggaaaggca gcgtcacgtc atcgcaattg gattcaaatg cgtgcctgct
     6961 ttcttctgaa atcctacgta gcttgcccca ctcgccaaga tgcagtaaag ccaatccctg
     7021 gtaataatgc ccgaccatga acatatagcc gtctccgagg cttctggata gctccagggc
     7081 tttgtccgcg gttgccgacg cacgccggta attcgaagcc agaacctcca catagatgtg
     7141 ctgtgtcaac ctggagtgca aaacaagtgg atttccgctg actcgggcct gatccatcgc
     7201 attgtcacag gcctccgcac agtccgcccg ccaagaccct agcatgaggt ttaggccgcc
     7261 ccacatgccc tttacaatgg attgaaggat cttgtcctca aggtggttgc tgcgtgccag
     7321 cgcctgttcc gccgtttcaa ggcatttacg gcgatcgagc caaacgagga cacggctcag
     7381 ttccagcaac gccagaacct cggcacggtt atcacccgcg gctcttgctg ttacgagcat
     7441 gagttccaga tcagcctttg cgtcgctcat ttcgcccatc gatcgccgga tagccgaagt
     7501 ctgtctcaaa agagctatcc gtgtctccgc ctgcttttcc tcgggcaacc gctcaaccat
     7561 ggcaagcgca cgtgcaaggt aatcgtaggc ttcgcggttc gcaaacctcc gtgccgcgtt
     7621 gttggctgcc tgggtcaggt aaaagactgc gcgctcccaa ccccatcctt tctcgaaatg
     7681 cagggcgagt tcagcggcat gcttgagatc gttttgacca tgcagccttt cgaggcattc
     7741 gccgatccgc aagtgcatcc ggatgatctg actggcagaa agcctctgat agagcgcctc
     7801 gacatatagg gcatggcgga atatgtaagt acaggcaacc tcgccttgcg gattttgctc
     7861 catgccatcc atacccaata tctggccacg cctggcgagt gcatcgcaac agcgatccac
     7921 ctccgctaca tccatgtcga gcacagtgcc gagtaacagg ggagagaact gcgaacctat
     7981 ggcactggcc acccccaaca gccgctg
//
LOCUS       DASSHJ010000267         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_59269, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000267 DASSHJ010000000
VERSION     DASSHJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59269"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFI43_10290"
     CDS             <1..181
                     /locus_tag="VFI43_10290"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7062554.1"
                     /translation="AYIDHEVAYHQQVLDAVDKTLIPSAKNEELKALLVKVRPAFVSH
                     LDHAKKVKEMLHKKH"
     gene            484..2151
                     /locus_tag="VFI43_10295"
     CDS             484..2151
                     /locus_tag="VFI43_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET7062555.1"
                     /translation="MPSRSPKSASNESDREWWKKTTVYHIYLRSFLDTNNDGIGDIGG
                     VIEKLDYLQELGYETIWVSPFTQSPQRDFGYDISDYFSIAHTYGDMAIFEELLREMHS
                     RNMKLVFDLVLNHTSDEHPWFKESASSRDNPKADWYIWRDGKGRSGMTPPNNWRAMAG
                     NKAWTYHPGRKQFYYTAFLPFQPDLNYHNPEVKQAMFDVVRFWLSKGVDGFRLDIISA
                     IYEDPDLTPNPASLRLTPSDGSLSVFFQHLKNNFLHEKSFEFATELRSVVDEFDNPKR
                     VLIGESHGDESVISRFCRTNEKDGLHAVFLFKAISTPFRAENYRKMLMTFEEHFPEPL
                     TPTLVFANHDRTRIISRLGGSVAKAKLLALFQFTCRGIPFTYFGDEIGIPRVRIPLRQ
                     GKDAIAIQHKWVPQPLADRSVEILNRDECRTPMLWNSEPNAGFCHHSMKPWLPLADNF
                     QEINVEKQTANPHSLLNLYKKLIRLRNETPALHEGSLEIANELCDKNVLAYCRISGTQ
                     KYMVMLNMSKYRVKNSLNGEMALSTHPESETSQLQPFEGRVMKPGNF"
     gene            complement(2167..2334)
                     /locus_tag="VFI43_10300"
     CDS             complement(2167..2334)
                     /locus_tag="VFI43_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062556.1"
                     /translation="MNGYLNSFGVFERQFGELDAVASGSFALELLRKKRNVAKVHDGK
                     CGGAERHVLEL"
     gene            complement(2818..3225)
                     /locus_tag="VFI43_10305"
     CDS             complement(2818..3225)
                     /locus_tag="VFI43_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062557.1"
                     /translation="MEADLPIKAPNHYDLSGRNIHISYSTTGIDGKPHFTYQDLQQTR
                     SFNGDQIRRVEIEVATIVSITFAPSVDFGGHTFSVLIPRINLLGAPNARETVRTYGIT
                     TLHRFATPPLPETGQRDFYTVARLDGTASQIAF"
BASE COUNT          879 a          925 c          854 g          797 t
ORIGIN      
        1 ggcgtacatc gaccacgaag tggcttacca tcagcaagtc ctcgacgcgg tggataaaac
       61 gctgattccg agcgcaaaga acgaggaatt gaaagccctg ctggtcaaag tgcggcccgc
      121 gtttgtgtcg cacctcgatc acgcgaaaaa ggtcaaggag atgctgcaca agaagcactg
      181 agcacgtatt cgtatcctcc tgctggagaa accgggataa gtcctctgcc gattcctgat
      241 ggtaacgaga gctgttggca ctgccgtcgc gagcagaacc gatcccgggt ttctctggcc
      301 cctgccgcta aagaccctgc atgctttttt ttaatgtaaa cttccccggt ttgcgttcca
      361 atcacttcct ccgcgtgata agtccagagt atccggaata aagctctgac ttatacttca
      421 agcaacagcc agatagtttc aagttcttcc gtcatcgata caagctcctt ccaggtggag
      481 gttatgcctt cccgttcccc aaaatccgca tcgaacgaat cggatcgcga gtggtggaaa
      541 aaaacgaccg tctaccacat ctacctccgc tcgttcctcg acaccaataa tgatggcatt
      601 ggagatatag gcggggtaat cgaaaagctc gattacctcc aggaacttgg ctacgaaaca
      661 atctgggttt cgccattcac tcaaagccct cagagagact tcggctacga tatcagcgac
      721 tatttctcca tcgcccacac ttatggcgac atggcgattt ttgaggaact attgcgagag
      781 atgcactcaa gaaacatgaa gcttgtgttc gacctcgtgc tcaaccatac atcagacgag
      841 catccctggt tcaaggaatc cgcaagctcc cgggacaacc ccaaggctga ctggtacatc
      901 tggagggatg gaaaaggcag aagcggtatg acaccaccca ataactggcg tgcaatggcg
      961 ggcaacaagg cgtggaccta tcaccccggg cgcaagcagt tctattacac ggccttcctc
     1021 ccttttcagc ccgacctgaa ttaccacaac ccggaggtca agcaggcgat gttcgacgtc
     1081 gtcagattct ggctcagcaa gggggtcgac gggtttagac tcgatattat cagcgccatt
     1141 tatgaagatc ccgatctcac gcctaacccg gcaagcttga gattgacccc ttctgacgga
     1201 tcactgtcgg tctttttcca gcatctgaag aacaattttc ttcatgagaa aagcttcgaa
     1261 ttcgccaccg agttgcggag cgtcgtggac gaatttgaca acccgaagag agtactgatc
     1321 ggcgaatccc acggagacga gtctgtaatc agccgatttt gccgcacgaa tgagaaggat
     1381 ggcctgcacg ccgtttttct cttcaaggcg atctcaaccc cttttcgggc cgaaaactac
     1441 cggaaaatgc tgatgacttt cgaagagcat ttccccgaac cgctgacacc caccctcgtc
     1501 ttcgccaacc atgaccggac acgaatcatc agccgtctgg gcggaagcgt ggcgaaagcc
     1561 aagttgcttg cccttttcca gttcacttgc aggggcatcc cttttaccta tttcggtgat
     1621 gagatcggca tacccagggt aagaataccg ctccggcagg gaaaggacgc tattgccatc
     1681 cagcataaat gggttcccca gccgctggct gaccgcagcg tcgaaattct caaccgggat
     1741 gagtgccgca cacccatgct atggaactcc gagccgaatg ccggcttctg tcatcactcc
     1801 atgaaaccct ggttgccgct cgcggataac ttccaagaaa taaacgtcga aaagcagacg
     1861 gcgaacccac actcgcttct taacctttac aagaagctga ttcgccttcg caacgaaacc
     1921 cccgccctgc acgagggaag cctcgaaatc gcaaacgagc tgtgcgataa aaatgttctg
     1981 gcatattgcc gtatttccgg tacccagaaa tacatggtca tgctgaatat gtcaaagtac
     2041 cgggtaaaaa attccttgaa tggcgagatg gcactgtcta cccatcctga aagcgaaacc
     2101 agtcaactgc aaccctttga aggaagggta atgaagccgg gaaattttta aaaaaatatc
     2161 gagattttac aactcaagaa catgccgctc ggccccgccg catttcccgt cgtggacctt
     2221 ggcgacgttg cgcttcttcc gcaatagctc caaggcaaaa gagcctgatg cgactgcatc
     2281 cagctcgccg aactggcgct cgaaaacacc gaagctgttg aggtaaccgt tcatcgacgg
     2341 gactggtctg attcaattgg aggcctagtc cagcaggtat ggctgcctgc ttcgacaacc
     2401 atacccttta cttcgcatgc atgttaaagc ggtccgatta cgctcttccc cgttccacgc
     2461 attgggaaac agtcccagat agttcgccgg tgcggaatgg agttaggcgc tgaattcctt
     2521 gcatatcgca gttgtcgcga agtcgaacgg ctacccctct ggaacgtcgg gtaggaaaaa
     2581 tccaaagtcg gtgtcgctgg tatcaaccga ctttatcaag gcaatcgata tgagagagtc
     2641 cccacgtttt ctcggtgctt acctcatccg ttttaaaatc atgtgacgtc gatacgccct
     2701 tgtagtcagt gcgacaagct ccgatgcgac gccagttgtg tacacacctc aatgtggtgc
     2761 cctttcagaa taattgcagc gccatgataa gcctctattc atgcggttgt tatacgatta
     2821 aaaagcgatc tgagacgcag tcccatccag gcgcgcgacc gtgtagaaat cgcgctgccc
     2881 ggtttctgga aggggtggcg tggcaaacct gtgaagagtt gtaatgccat aagtccgcac
     2941 tgtttctcgt gcattcggtg cacccaataa atttatcctg ggtatcagaa cactaaaggt
     3001 gtggccccca aagtcgacgc tgggtgcaaa agtaatgctg acaattgtgg caacttctat
     3061 ctcgacacgc ctgatctgat ccccgttgaa gctgcgcgtt tgctgcaaat cctgataggt
     3121 aaaatgcggc ttgccgtcta taccggtggt ggagtacgag atatgtatgt ttctgccgct
     3181 gaggtcgtaa tggttaggcg cctttatagg caaatcggct tccattgaag actccttggg
     3241 tgatatagca gcggaagagc gctgcccccg tgtcaggaat ttttttcgaa gcgacacgta
     3301 cgcactagtc ttgtaccgct tgagaactcc aggcacgctt catacgataa acaaggccgc
     3361 gccaagagtc ataatggaat ggttaaatat tggttaaata ttggttaaat ttcgaagctg
     3421 tgaggaacaa ggattgatgg atataaaacc gagtg
//
LOCUS       DASSHJ010000268         5292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_596207, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000268 DASSHJ010000000
VERSION     DASSHJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596207"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2498
                     /gene="leuS"
                     /locus_tag="VFI43_10310"
     CDS             <1..2498
                     /gene="leuS"
                     /locus_tag="VFI43_10310"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7062558.1"
                     /translation="SGKLHMGHVRNYTIGDVLSRYWRMQGYNVLQPMGWDAFGLPAEN
                     AAMQNHVSPARWTYDNIAYMRKQLQSLGLAIDWGREFATCQPDYYRWNQWFFLRMLEK
                     GLAYRTTGTVNWDPVDQTVLANEQVIDGRGWRTGALVEKREIPMYYLRITTYADELLE
                     SLDKLPGWPERVKTMQANWIGKSYGVDVTFPPDAESGMSQPLKVFTTRADTLMGATYV
                     AVAPEHPVALHAAEHNPELAEFIEACSHGATMEAELATQEKKGLDTGLSVIHPLTGER
                     LPVWVANYVLMGYGEGAVMAVPAHDERDFEFASKYGLPIKPVIKPLDSDLPVPLLEAY
                     VEHGVTFDSGEFSGLGFQAAVDVIAAALEKKGLGEKKVHYRLRDWGISRQRYWGCPIP
                     LIYCDGCGIVPVPDDQLPVLLPEDLVPDGSGNPLAKTPSFVECVCPRCNQPARRETDT
                     MDTFVDSSWYYIRYACADDKEAMTDERVDYWLPVDQYIGGIEHAILHLLYSRFWTKVM
                     RDFGLVKFDEPFLNLLTQGMVLNEIMFRKTETGRLIYFNPAEVDSQIDEQGRRVGAVL
                     RADGHPVELGGIGTMSKSKNNGVDPQRIVEEYGADTARLFMMFASPPEQTLEWADTGV
                     EGAFRFLKRLWRQVYEHVEQGEHEQQAKAAGASENHDKNAGLNADLKALRFQLHQTVA
                     KVSDDLGRRHTFNTAIAAVMELMNALAKLDNSSAAARSLMQEALEKLVLLLSPIVPHI
                     CHALWRELKPGTTLLDQPWPQADPAALVRNEVELVVQVNGKLRGRICVATDAKQTDIE
                     QLALESDQVEKFIAGKKVKKVILVPGRLVNIVI"
     gene            2564..3424
                     /locus_tag="VFI43_10315"
     CDS             2564..3424
                     /locus_tag="VFI43_10315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET7062559.1"
                     /translation="MTKIHLLHGDCREELQKLADASVDLIVTSPPYADQRKRTYGGIP
                     PDEYVAWFRPISAELLRVLKPSGTFMLNIKERVVNGERHTYVLELILTMRAQGWLWTE
                     EFVWHKKNCYPGKWPNRFRDAWERLLQFNKQKKFKMLQDEVMVDTGDWAKTRLRNLSV
                     TDRVRDESRVGSGFGKKTENWLTRDKAYPTNVLHLATECSNRDHSAAFPEALPAWFIK
                     LFTSPDDVVLDPFMGSGTTLRVAKRLQRDAVGIDIHRGYIDRMQAELMGPKRPDWAPL
                     IEFNSSKLPV"
     gene            3421..3921
                     /gene="lptE"
                     /locus_tag="VFI43_10320"
     CDS             3421..3921
                     /gene="lptE"
                     /locus_tag="VFI43_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET7062560.1"
                     /translation="MNSKILGVFAFILFALTACGFQLRGQATLPFDTLFIAFPAGQPL
                     GTDLKRLVAAGTKTRIVDDPKNAQATLEIVSVANNKEIMSVSGGGRVREFELRYRVSF
                     RLTDAKGTELIPTNEIALRRIMPFTDQQVVAKEGEEAMLVREMQSDSAAQIVRRLEAV
                     KTASAS"
     gene            3964..4980
                     /gene="holA"
                     /locus_tag="VFI43_10325"
     CDS             3964..4980
                     /gene="holA"
                     /locus_tag="VFI43_10325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7062561.1"
                     /translation="MRVAADQLPHHLSRKLASLYTVFGEDLLLTIEASDLIRARAREA
                     GYAEREIFSIDHHFNWAELQQRSSSLSLFGERRIMDMRIPSGKPGVKGSAAIEEYCRA
                     LPPDTVTLVTLPKMDRQNSGAKWFKALEAAGVMVPVYPVERGRLPSWIGQRLGMQGQS
                     ADPDTLRFIAENVEGNLLAAHQEIKKLALLYPPGTLSFSEVKDAVLDVARYDVFKLSD
                     AMLSGDTVRYLRILEELQGEGTPLPYIVFTLAGQIRSLITIRHGIEAGRPLPEMMRQI
                     KARQDQQKVLESAARRTSTRQLTDALLHLSKIDRISKGVGMGDPWDELLQLGLRFAMA
                     GHPQ"
BASE COUNT         1213 a         1365 c         1558 g         1156 t
ORIGIN      
        1 cgtccggcaa gttgcacatg ggacatgtgc gcaactatac gattggcgat gtgttatccc
       61 gctattggcg gatgcagggc tataacgtgc tgcaacccat ggggtgggat gcattcggcc
      121 ttcctgccga gaatgccgca atgcaaaacc atgtctcgcc cgcccggtgg acctacgaca
      181 acatcgcgta tatgcgcaag caattgcaga gcctaggcct tgcaatcgac tggggccgcg
      241 agttcgcgac ttgccagccg gattactatc gttggaacca gtggtttttc ctgaggatgc
      301 tcgagaaagg gttggcttac cggacaacgg gcaccgtcaa ctgggaccct gtcgatcaaa
      361 cggttctcgc gaatgaacag gtgatcgatg ggcggggctg gcgcaccggc gcgctggtgg
      421 aaaagcgcga aatcccaatg tattacttga gaatcactac ttacgccgat gaactgctcg
      481 agtccctgga caaactgccg ggctggccgg agcgggtcaa gaccatgcag gccaactgga
      541 tcggcaaaag ttacggggtc gatgttacgt tcccgcccga cgccgaatcc ggcatgtcgc
      601 agcctctcaa ggtttttacc acccgggccg acacgttgat gggcgcgact tatgtggcgg
      661 tagcccccga gcacccggtt gctttgcatg ctgcagagca caatcctgaa ctggccgagt
      721 tcatcgaggc gtgcagccat ggggcgacaa tggaagccga gcttgcgacg caggaaaaaa
      781 aggggctcga tacaggactg tccgtcattc atccgctgac cggcgaaagg cttccggtat
      841 gggtcgcgaa ttacgtattg atgggttatg gcgaaggagc ggtgatggcg gtgcctgcgc
      901 acgacgagcg ggacttcgag tttgccagta agtacggact gcctatcaag cctgtgatca
      961 agcctctcga ttcagacctg cctgttccgt tgttggaagc ctatgtggag catggcgtca
     1021 cgttcgattc cggcgaattt tcggggcttg gattccaggc ggcggtggac gtaatcgctg
     1081 cagcgttgga aaagaagggc ttgggggaaa agaaagtaca ttatcggctg cgggattggg
     1141 gaatttcccg ccagcgctac tggggttgcc ccatccctct catttattgc gacggatgcg
     1201 gcatcgttcc agtgccggac gaccaactcc cggtgttgct tcctgaagac ctggttcccg
     1261 acggctccgg caatccattg gcgaagaccc cttcgttcgt ggagtgtgtt tgtccgcgat
     1321 gcaatcagcc cgcgcgacgg gaaaccgata ccatggatac cttcgttgat tcttcctggt
     1381 attacattcg ctatgcctgt gccgacgaca aggaagccat gacggacgag cgggtggatt
     1441 actggctgcc agtcgatcag tatatcggag gcatcgagca cgccatattg cacttgctct
     1501 attcgcgctt ttggaccaag gtcatgcgcg actttggcct cgtcaagttc gatgagccat
     1561 ttctcaacct gctaacgcaa ggcatggtgc tgaatgaaat catgttccgg aagaccgaaa
     1621 caggccggct gatatatttt aatcccgccg aggtcgatag ccagatcgat gagcaggggc
     1681 ggcgcgtcgg ggccgtgctg cgcgcggatg gccaccccgt ggagctgggc ggaatcggta
     1741 ccatgtccaa gtcgaagaat aacggggtcg accctcagcg gatcgtggaa gagtacggtg
     1801 ccgatactgc gcggttgttc atgatgtttg ccagcccgcc ggagcagacg cttgaatggg
     1861 ctgacacggg tgtggagggt gcattccggt ttttgaaacg gttatggagg caggtttacg
     1921 aacacgtcga gcaaggtgaa catgagcagc aggctaaggc cgcaggcgct tcagaaaacc
     1981 atgataaaaa cgccggtctc aacgctgatc tgaaggcctt gcgttttcag ctgcatcaga
     2041 ccgtggcgaa agtaagtgat gatcttggca ggcgtcacac attcaatacg gcaatcgctg
     2101 ccgtgatgga actcatgaac gccttggcga agctggacaa ctccagcgcg gccgcgcgca
     2161 gcctgatgca ggaagcattg gagaagctcg tgctgctgct gtcgcccatt gttccccata
     2221 tctgccacgc gctatggcgt gagctcaagc ctgggaccac gctgcttgat cagccgtggc
     2281 cccaggcgga ccctgcggcc ctcgtgcgga atgaggttga gcttgtcgtg caggtcaacg
     2341 gtaaattgcg gggccgcatt tgcgtcgcca ccgatgcgaa gcagacggat atcgagcaac
     2401 ttgcactgga aagcgatcag gtagagaagt tcattgctgg aaagaaggta aaaaaagtca
     2461 ttttggtacc cggcagattg gtcaatatcg ttatatagta gcgtgacggc agtcacccca
     2521 ttgagcagcg cgggcattcc ggttctgcta ttacatttct tccatgacaa aaattcacct
     2581 cttgcacggc gattgcaggg aagagttgca gaagctcgct gacgcttcgg tggatctgat
     2641 cgttacttct ccgccttatg ccgatcaaag aaagcgtact tacggtggta ttcctcccga
     2701 cgaatacgtg gcatggttcc gacccatctc tgccgagctt ctgcgcgtac tgaagcccag
     2761 cggtactttc atgctcaata tcaaagagcg ggtcgtcaat ggggaacgcc atacctacgt
     2821 cctggaacta attttgacaa tgcgcgccca aggatggtta tggaccgagg agttcgtgtg
     2881 gcataaaaaa aattgttatc cgggaaaatg gcccaatcgt ttccgcgatg cctgggagcg
     2941 tttgctacag ttcaacaagc agaaaaaatt caaaatgctg caggacgagg tcatggtcga
     3001 cacgggggat tgggcaaaga ccaggctgcg taacttgagc gtgacggata gggtgcgaga
     3061 cgagtcaagg gtaggtagcg gcttcggcaa gaaaacggaa aattggctga ctcgcgacaa
     3121 ggcgtatcca accaatgtac tgcatcttgc cacagagtgt tcaaacaggg atcacagcgc
     3181 tgcatttcct gaagctttgc ccgcgtggtt tataaaattg tttacttcac ctgatgatgt
     3241 tgtgcttgac ccgttcatgg gatcagggac gacattgcgg gtggcaaagc gcttgcaacg
     3301 cgacgctgtt ggcattgaca ttcaccgcgg ctatatcgat cgcatgcagg cggagctgat
     3361 ggggcccaag cgccccgatt gggctcccct gattgaattt aactcttcta aattaccggt
     3421 atgaattcaa aaattcttgg agttttcgca tttatcctgt ttgccttgac ggcctgcgga
     3481 tttcaactgc gcgggcaggc aacgttgcca tttgacacgc tttttatcgc ttttcccgct
     3541 ggccagccgc tcggcactga tctcaaacgt ctcgtagcgg cgggaaccaa gacacggata
     3601 gtcgatgacc cgaaaaacgc gcaggccaca ctcgaaatcg tgagtgtcgc caataacaag
     3661 gaaatcatgt cggtgtcagg aggcggccgc gtgcgggagt tcgagctccg ctaccgcgta
     3721 tctttccgtc tcacggatgc caagggcacc gaactcattc ccacgaatga aattgccctg
     3781 cggcgcatca tgccttttac cgaccagcag gtcgtcgcga aggaaggaga ggaagccatg
     3841 ctggtcaggg aaatgcagag cgattcagcc gcccagatcg tgcgccgctt ggaggcggta
     3901 aaaacggcat cggcatcgtg atcgtcttgc ggtcgtagag gcactgctct caggcccatg
     3961 cccatgcgtg tagccgcaga tcagcttcct caccaccttt cgaggaaact cgcttcgctt
     4021 tacacggttt ttggcgaaga tctgctgctc acgatcgaag cctccgatct aattcgcgcg
     4081 agagcgcgtg aagcgggcta tgcggagcgc gaaatcttct cgatagacca tcatttcaac
     4141 tgggccgagc ttcagcagcg cagcagcagc ctgtccctgt ttggcgagcg gcgtataatg
     4201 gatatgcgca taccttccgg caagccagga gtgaaaggca gcgccgcgat cgaggaatat
     4261 tgccgcgcgc tgccgccgga cacggtcacg cttgttacgc tgccgaagat ggacaggcag
     4321 aattcagggg caaaatggtt caaggccctg gaagcggcag gggtaatggt cccggtatac
     4381 ccggtggagc gtggccgttt gccttcatgg atcggccagc gccttgggat gcaagggcaa
     4441 agcgctgatc ccgatacttt gcgctttatt gccgagaacg tggaaggtaa tctccttgca
     4501 gcccatcagg agatcaagaa actcgccctg ttatatccgc ccggtacctt gtccttcagt
     4561 gaagtcaagg atgcggtatt ggatgtcgcg cgttatgatg tattcaagct ttccgatgcg
     4621 atgctttccg gggatacggt ccgttatcta cgcatactgg aggaactgca aggggaggga
     4681 acacctctgc cctacattgt cttcacgctg gcggggcaga tacgctcgct gattacgata
     4741 cggcacggga tcgaagccgg ccggcctcta cccgaaatga tgcgtcaaat caaggcaaga
     4801 caagatcagc aaaaagtgct ggaaagtgca gcgcgccgca caagtaccag gcaactgacg
     4861 gacgccttgt tgcatttgtc gaaaatagac aggatcagca agggtgttgg catgggcgat
     4921 ccttgggacg aactgctgca actgggactg cgattcgcga tggcagggca tcctcagtaa
     4981 tatcgctttg gcgaagctct ggcagaaaca catagaccaa taccaaggtt cggggtttgc
     5041 tacagtcgac gggctgatat gtaggctgca tgtattaaat gcgttcaaac gaatttaagt
     5101 tgaattcgaa tccaactcaa attggattct aattccaata cggaggcgcg ccgataaagc
     5161 agcgcatatc tatcaaatca tgtattgaat tgttctcaat aaggggaacg gagacatgga
     5221 cattatcgac gtcaagactt atatgcaatc gattggacgg gaagcacgcg cagcttctcg
     5281 cctgatagcc aa
//
LOCUS       DASSHJ010000269         6727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1256675,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000269 DASSHJ010000000
VERSION     DASSHJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256675"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFI43_10330"
     CDS             <1..795
                     /locus_tag="VFI43_10330"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HET7062562.1"
                     /translation="PVAKRVSRATVCNRCFEQGGLMDGQHKKVAILQSNYIPWKGYFD
                     LIHDVDLFVFYDDVQYTHSDWRHRNKLMTTQGPQWLSIPAGHDLKRLICEVEVADQKW
                     KQKHRRTVEQNYRKAPFFKEQEPLLDFLYENAITNLSEYNQRAIKHLSGLLGIATQFT
                     DSRLLSAHGSKTERLISILRKVGAGTYISGPSAKSYIDETLFAEAGIELAYKQYPDYP
                     AYPQCSSPFFPFVTLLDLLFNVGRDAPYYIWGWRDQRDSGFDDLRP"
     gene            792..1154
                     /locus_tag="VFI43_10335"
     CDS             792..1154
                     /locus_tag="VFI43_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7062563.1"
                     /translation="MSYFYIFLTIAFTVYGQIVLKWQVIDAGHFPENTLDKVLFLVRL
                     VLNPWVISGFLAAFAASLTWMVAMTKLELSHAYPFMSLNFVFVIILSYLLFQETITTP
                     KILGLGLIILGIIVGSRG"
     gene            1151..2020
                     /locus_tag="VFI43_10340"
     CDS             1151..2020
                     /locus_tag="VFI43_10340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7062564.1"
                     /translation="MKICIFCEERFASPNWVCPSCDSSPQYLDGYLSFAPEQARSSEG
                     FQPHHFAELANAEAGNFWFRSRNRLITWAIQRFFPAATSFLEIGCGTGFVLSGVTHKS
                     PHLSVCGSEIHSSGLDFAATRVKQAKLFQMDARNIPFEDEFDIVGAFDVLEHIEEDEV
                     VLGQIHRAVSPGGGAIFTVPQHRFLWSQQDEYACHVRRYEAGELLDKVKRAGFQVERT
                     TSFVALLLPLMFLSRLSKRKTIENFDATQEMRISRSLNAILERMLDLERMIIRAGISL
                     PVGGSRLLIARKP"
     gene            2027..3169
                     /gene="rffA"
                     /locus_tag="VFI43_10345"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             2027..3169
                     /gene="rffA"
                     /locus_tag="VFI43_10345"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HET7062565.1"
                     /translation="MVIPFNKPYMTGKELWYVSQAHANGHLAGDGIFTKKCNKWLEQR
                     IGCKKALLTHSCTAALEMAAMLARIQAGDEVIMPSYTFVSTANAFALRGAIPVFVDIR
                     PDTMNIDEALIEAAITPKTRAIVAVHYAGVSCEMDTIIAIAEKHGLLVIEDAAQGIMS
                     TYKGRPLGSIGHFAALSFHETKNIISGEGGALLVNDERFTEQAEIIREKGTNRSLFFR
                     GQVDKYTWVELGSSYLPSELIAAFLWAQMEEADAITGRRLDLWNGYHQSFADLEEKGK
                     IRRPVIPSDCSHNAHMYYLLLPSLSARTSFIESLKQKEVQAVFHYVPLDSSPMGEKYG
                     RKSGELKQTEMMSERLVRLPLWLGLEEYQGDIIDKVIVELGDRGHN"
     gene            3156..4841
                     /locus_tag="VFI43_10350"
     CDS             3156..4841
                     /locus_tag="VFI43_10350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7062566.1"
                     /translation="MAITDNAVNPGRVEADVRRSALNPADAASILLLWGVVCLLFWDT
                     LDAGEFWWTDESRHAMNGVFFFDLWRDMPWRAPYEYALQYFAQYPALALNWYLPFFPV
                     VESLFFALFGINELGARLTVLLFTLIGVTAWYAWTQPIWGRPAAILSCLLYLSAPGTV
                     DWTRSVMLEAPAVAMIIVSVLAFDRYLDRPSLFRSVTAGLLLGCMLLLKQTTAFILPA
                     LLAYAYFTGRKSLLVTKQAAAAYLIVAAVVGVVAVHALKFGSVPVTSLVDQFSSERSE
                     LFTLPRWQQFWWALWAVCGLPLVLAILAGIPGIRIKKTPQDLLILFWLAGSCLFTTIV
                     IAVGPGNVPRYIMYALPALALLACKPLSQTFKNTSISRIAVVFLGMLTAFNVYSAWAK
                     PHPFVSGYQQAAEFVAQQPDTGLILFAGKHDGNFIFHLRAADPERDKAVLRADKILVS
                     MAVQKSFGMVSYVRSEQDVLDLIDRFGIGTIVIESPDVVDLPEFKLLAQTLEDKRFQM
                     IKEIPVVTNVAEFSNLSLRVYRYLEKKEQEGDVIIPLPHSGMEIKLKPRKNSR"
     gene            4886..6103
                     /locus_tag="VFI43_10355"
     CDS             4886..6103
                     /locus_tag="VFI43_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET7062567.1"
                     /translation="MRILHILDHSIPLHSGYTFRTLSILKEQRNRGWETFHLTSPKHG
                     NPIFLEEDVEGWHFYRTPTAKGLLSRLPVLNQAAVMSQLTARLHEVAQIVRPDVLHAH
                     SPVLNALPALRVGQALKIPVVYEVRAFWEDAAVDHGTSREWGMRYRLTRGLESYALKR
                     ADAVTTICEGLRGDILDRGIPAEKVTVIPNAVNIENFSMAGRPDVKLAADLGLHENIL
                     LGFIGSFYAYEGLAVLMQALPEMLVRNPKIRVLLVGGGPQEKELKALATQLGVEKKVV
                     FTGRVPHDHVQAYYNLIDILVYPRLKMRLTDLVTPLKPLEAMAQGRLVLASDVGGHRE
                     LIQDGKTGMLFPAGNPDALASKALELLSAEELWPGLRSEARSFVEKQRNWPISVARYR
                     DIYARLAPGPASP"
     gene            6160..>6727
                     /locus_tag="VFI43_10360"
     CDS             6160..>6727
                     /locus_tag="VFI43_10360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7062568.1"
                     /translation="MANQTLQLARLLRQENIGVDLIQANRPYHPEWIGRFRGIRAAFR
                     LVAYIAHLWRSAGKVQLFHIMANSGWSWHLFAAPAIWIARMRGKAVVVNYRGGEADSF
                     FNKSFLWVKLSLSRADAIVVPSGFLERVFERRGFSTKIVPNIIDLSRFAAGTRTHQAN
                     GPGSPHIIVTRNLEPIYDNATALRAFSIV"
BASE COUNT         1539 a         1766 c         1808 g         1614 t
ORIGIN      
        1 ccagtggcga agcgggtatc ccgagccacc gtctgcaatc gttgctttga gcaaggcgga
       61 ctgatggacg ggcagcacaa gaaggtcgcc atactccaat ccaactatat tccatggaag
      121 ggctatttcg atctcatcca cgatgttgat ctcttcgtct tctatgacga cgttcaatat
      181 acccactcgg actggcgcca cagaaacaag ttgatgacga cgcaaggccc ccagtggtta
      241 tcgataccag ctgggcacga cctcaaacgc ttgatatgcg aagtggaggt cgccgatcag
      301 aaatggaagc aaaagcatag aaggaccgtc gaacagaatt acagaaaagc gccgttcttc
      361 aaagagcagg aaccgttact ggattttttg tatgaaaacg cgataacgaa cctttcagag
      421 tacaaccagc gcgccattaa acatctatca ggcttgcttg gcattgcaac acaatttacc
      481 gatagccggc ttctctcagc gcacggcagt aaaaccgaaa ggttgatcag catattacgg
      541 aaagtcgggg caggcactta tatcagtggc ccttctgcca aaagctatat tgacgagacg
      601 ttgtttgcag aagccggtat agaactcgcc tataaacaat accccgacta cccagcttat
      661 ccacaatgtt cttccccatt ttttcctttc gtcactctgc ttgacttgct attcaacgta
      721 gggcgcgacg ctccttatta tatttggggt tggcgagacc agcgggacag cgggttcgac
      781 gaccttcgac catgagttat ttttatattt tccttactat tgcttttacc gtttacggcc
      841 agatcgtgct gaaatggcaa gtcatcgatg cgggtcattt tcccgagaat acgctggata
      901 aggttctatt tctggtgcgg ctagtactta atccctgggt tattagcggg tttctagccg
      961 cgtttgccgc ttccctcacc tggatggtgg ccatgaccaa gctggaatta agccatgctt
     1021 atcccttcat gagccttaat ttcgtgtttg ttatcatcct gagctacctg ctgttccagg
     1081 aaaccattac aactcccaag attctgggct tgggcttgat catcctgggt attatcgtgg
     1141 ggagcagagg ttgaaaatct gcattttctg tgaagagcgc ttcgccagtc caaactgggt
     1201 gtgcccttcc tgtgacagca gcccccagta tctcgatgga tatctaagtt ttgcgccgga
     1261 gcaggcacga tcaagcgaag gattccaacc ccatcatttt gctgaacttg ccaatgccga
     1321 ggcagggaat ttctggtttc ggtcgcggaa ccggctcata acctgggcga tacaacgttt
     1381 ttttcctgcc gcaacaagct tccttgaaat tggatgcgga acaggatttg ttctttcagg
     1441 cgttacccac aagagccccc atctttcggt ctgcggaagc gaaattcact catccgggct
     1501 tgactttgct gccacacgtg tcaagcaagc gaaattgttt caaatggatg cgcgtaatat
     1561 tccatttgag gatgaattcg atatcgtggg cgcattcgat gtactagaac atatcgaaga
     1621 agacgaagtg gttctgggac aaatacaccg cgcagtttct cccggcggcg gggcgatctt
     1681 tacggtaccc cagcaccgct tcttgtggag ccagcaggat gagtatgcat gtcatgtgag
     1741 acgctacgaa gccggggaac tgttggataa ggtgaaaagg gctggctttc aggtggaacg
     1801 aacaacctct ttcgtagccc tgctattgcc gctgatgttt ttatcacggc tatccaaacg
     1861 taaaacgata gagaattttg acgcaacaca ggaaatgaga ataagcaggt ccttaaacgc
     1921 gatattggaa agaatgctcg atctggaacg tatgataata cgagcaggaa tttccctccc
     1981 cgtcggcgga tcaagattgc tcattgcccg caagccctag gaccacatgg ttataccctt
     2041 taacaagcct tacatgacgg gaaaggagct atggtatgta agccaggctc atgcaaacgg
     2101 tcatctggcc ggagatggta tttttaccaa aaaatgcaat aaatggctgg aacagcgcat
     2161 cggctgtaaa aaggcgctcc tgacccactc ctgcactgcg gcgctggaaa tggccgcaat
     2221 gctcgcccgg attcaggccg gtgacgaggt tatcatgcct tcgtacacct tcgtgtcaac
     2281 cgcgaacgca ttcgccctca ggggagctat tccggtattc gttgatatcc gccctgacac
     2341 gatgaacatc gatgaagcgc tgatcgaggc cgccattacc cctaaaacga gggccattgt
     2401 ggctgttcat tatgcggggg tgagttgcga gatggacacc atcatagcga tcgcggaaaa
     2461 gcatggattg ctggtcatcg aggatgcggc gcagggaatc atgtcgacct acaagggccg
     2521 cccactggga agtatcggcc atttcgctgc gctgagcttt catgaaacca agaacatcat
     2581 ctctggagaa ggcggtgcgc tgctggtcaa cgatgaacgc ttcaccgagc aggcagaaat
     2641 cattcgggaa aaaggaacca accgcagcct gttttttcga gggcaagtcg ataaatatac
     2701 ctgggtggag ttggggtctt catatttgcc cagcgaactg atcgctgcct tcttgtgggc
     2761 gcagatggaa gaggccgatg ccatcaccgg gcggcgcctc gatctctgga atggttacca
     2821 tcaaagcttc gccgacctgg aagagaaggg aaaaatccgg cgccctgtta ttccatccga
     2881 ttgcagccac aacgcccata tgtattacct cttgttgcct agcctgtcag cgcgcacgtc
     2941 gtttatcgaa agcctcaagc aaaaggaagt ccaggcagtc ttccattacg ttcccctcga
     3001 tagctcaccc atgggggaaa aatatggacg aaaatcgggg gagctgaagc agacagaaat
     3061 gatgagcgag cggctggtga gactgccact gtggctaggg ctggaagaat accagggcga
     3121 tatcatcgac aaagtcatag tggagctcgg agaccgtggc cataactgat aatgccgtaa
     3181 accccgggcg ggtggaggct gacgtacgga gaagcgcgct caatccggcc gatgcagcat
     3241 cgatcctgtt gctatggggg gttgtctgcc tgctgttctg ggatacgctg gacgccggcg
     3301 aattctggtg gaccgacgaa tcacggcacg cgatgaacgg ggtgttcttc tttgatttat
     3361 ggcgggatat gccatggagg gcgccgtacg agtatgcgct ccagtatttt gctcaatacc
     3421 ctgccttggc gcttaactgg taccttccat ttttccccgt ggtcgagtcg ctttttttcg
     3481 cattgttcgg catcaacgag cttggagcgc gtctgaccgt acttttattt acccttatcg
     3541 gagtaactgc ctggtatgcc tggacacagc cgatctgggg gcgtcctgca gccatacttt
     3601 cctgcctgct gtatctttca gcccccggaa ctgttgactg gacacgctcg gtgatgcttg
     3661 aagcaccggc tgtcgcgatg ataatagtct ccgtactggc cttcgaccgt tatctggacc
     3721 gcccctcgct gttccggtct gtgaccgccg gtcttttgct ggggtgcatg ctgctgctca
     3781 agcagaccac tgccttcatt ttgccggctc tgcttgccta cgcgtacttt actgggcgca
     3841 aatcactcct ggtgacgaag caagcggcgg ccgcatatct tatcgtagcc gcggttgttg
     3901 gcgtggttgc cgttcatgcg ctgaagttcg gatcggtccc ggtgacttcc ctggtcgatc
     3961 agttcagctc cgaaaggtcc gaattgttca cgctcccgcg atggcaacaa ttctggtggg
     4021 cgttgtgggc ggtctgtgga ttgccgcttg tgctcgcaat tcttgccggg attccgggta
     4081 tccggatcaa gaaaacgcca caagatttgc tgattctttt ctggctggcc ggatcctgtt
     4141 tatttacaac cattgtcatt gcggtagggc ccgggaacgt cccgcgttac atcatgtatg
     4201 cgttgcccgc tttggcgctt cttgcatgca agcctctttc gcagacgttc aagaacacaa
     4261 gcatatcccg gattgccgtc gtatttctgg gaatgctgac ggcatttaat gtctattctg
     4321 cctgggccaa accccatcct tttgtatcgg gatatcagca ggccgccgaa ttcgtggctc
     4381 agcaaccgga caccggcctg atcctttttg ccggaaagca cgacggcaat ttcatttttc
     4441 atttgcgcgc tgctgatccc gagagagata aagcggtctt gagagcagac aagatactgg
     4501 tatcgatggc agtgcagaaa tccttcggca tggtttctta tgtgcgttcg gaacaggacg
     4561 tccttgatct gatcgaccgc tttggaattg gaacaatcgt gatagaaagc cccgacgtcg
     4621 ttgatttgcc ggaattcaag ctactggcgc aaacgttgga ggacaagcgg ttccagatga
     4681 tcaaggaaat tcccgtagtc accaacgtcg cggaattttc caatctatcc ctccgcgttt
     4741 accgctatct ggagaaaaag gagcaggaag gggacgttat cattcccttg ccacactctg
     4801 ggatggaaat aaaattgaag cctcgtaaga attcgcgttg atttcgccga acggctgcct
     4861 tctgcaaatc cctcactcat cgtcaatgcg aatattacat attcttgacc actcgatacc
     4921 cctccacagc ggatatacct ttcgcacgct ttccatcctc aaggagcagc gcaatcgcgg
     4981 ctgggaaact ttccacctga cgagtccgaa gcacgggaac cctattttcc ttgaagagga
     5041 cgtggaaggg tggcattttt accgcacccc aacagcgaag ggtttattga gccggctccc
     5101 cgtcctgaat caggccgcgg tcatgtcgca actgacggcc cgattgcacg aggttgcgca
     5161 aattgtccgg cccgacgttt tgcacgcgca ttctcctgta ttgaacgcgc tgcctgcgtt
     5221 gcgggtggga caggcgctga aaatcccggt ggtatatgaa gtcagggcgt tctgggaaga
     5281 cgctgccgtg gatcacggca cgagccgtga atgggggatg cgctatcgtc ttacccgcgg
     5341 acttgaaagt tacgctctga aacgggcaga cgcggtaacg accatttgtg aaggtttgcg
     5401 gggagacata ctcgaccggg ggattcctgc cgaaaaggta accgtcatac ccaacgcggt
     5461 gaatatcgaa aacttcagca tggccggaag gccagacgtg aagcttgcgg cggatctcgg
     5521 cctgcatgaa aatatcctgc tgggtttcat tggctccttc tatgcttatg aaggcctggc
     5581 tgtcctgatg caggcattac cggaaatgct cgtgcggaac ccgaaaatca gagtcctcct
     5641 cgtgggcgga ggaccccagg aaaaagaact caaggctttg gcaacccagc taggtgttga
     5701 aaaaaaggtg gtttttacag gccgtgtccc tcatgaccac gtgcaggcat actataacct
     5761 catcgacatc cttgtctatc cgaggctaaa aatgcggctg accgacctgg tcacacccct
     5821 caagccgctt gaggcgatgg cgcaaggaag gcttgtcctt gcatcagacg tgggcgggca
     5881 ccgcgaattg attcaggacg gtaaaaccgg aatgcttttc ccggcaggaa acccggatgc
     5941 gctcgcatca aaagccctgg aacttctttc ggctgaagaa ctatggccgg ggctgcggtc
     6001 agaagcgcgc agcttcgttg aaaagcagcg caactggccc atcagcgtcg cgcgctaccg
     6061 cgatatttat gccaggctcg ctccagggcc agcctcccct tgaaaacttt gcccttatcc
     6121 atagggctgg ttggaccgct gccaccccct tcagggggaa tggcaaacca gaccctgcaa
     6181 ctcgccagat tgctgcggca ggagaacatt ggcgtggatc tgatccaggc caatcggcct
     6241 tatcatccgg agtggatagg gagattcaga gggataaggg ccgcattccg attggtcgct
     6301 tatattgctc acctatggcg ttcggccggg aaggtccagt tatttcacat catggccaat
     6361 tccggctggt cctggcacct tttcgcggcg ccggccatct ggatagcgag gatgaggggc
     6421 aaggccgttg tcgtcaatta ccggggtggc gaagcggata gctttttcaa caaatccttc
     6481 ctatgggtaa aactgagcct gagccgagca gacgcgatcg tcgttccctc cggatttctt
     6541 gaacgggtgt ttgaaaggcg cggtttctcc accaaaatcg tccccaacat tatcgatcta
     6601 agccgttttg ccgcgggaac ccgaacgcat caggcgaatg gtccaggctc tcctcatatc
     6661 atcgtaaccc gcaaccttga gccgatttat gacaatgcca ccgcgttgcg cgcattcagc
     6721 atcgtaa
//
LOCUS       DASSHJ010000270         5163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_756014, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000270 DASSHJ010000000
VERSION     DASSHJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756014"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFI43_10365"
     CDS             complement(<1..962)
                     /locus_tag="VFI43_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HET7062569.1"
                     /translation="MNRNYLATINRFSDLKVLVIGDAMLDVYLDGSANRICREAPVPI
                     VDIRNVKTMPGGAANTAVNLACLGAEVCYLSVVGDDHEAGLLQDALARRGLDLSFVIR
                     DSVRRTLTKQRVAAGSQLLVRFDAGTTEAIRMEYESRIIDILQEKFHEVDAVVVSDYG
                     YGIMTDKVIGTLGVLQRKKENILVIDAKSLDKYSRVGATVSKPNYQELVTLLSITEPA
                     PLGHRANQIRLYGQRLLRKTGAKYVAATIDIEGALLFQRGKEPYRTFSKPVENTKAAG
                     AGDTYVAALTLALASGAAIEDAGEIARSAAGIILQKSGTATCTQ"
     gene            complement(990..1598)
                     /locus_tag="VFI43_10370"
     CDS             complement(990..1598)
                     /locus_tag="VFI43_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7062570.1"
                     /translation="MALEAMKHPGLDKAVFIDKDGTLIPDVPYNVDPQHVRLTVGAGT
                     ALQRLRAEGYKLIVISNQSGIARGLFEEQDLMPVNLRIQALLAPYRVKIDAFYYCPHS
                     PADYCECRKPKPGMILQAARDHGIDLEISWMIGDILNDVEAGNRAGCRTIHLNNGNET
                     EWHEGNHRRPLHTVDDWLEAASIVCPTLAHVGQLKALFVATS"
     gene            complement(1589..2320)
                     /locus_tag="VFI43_10375"
     CDS             complement(1589..2320)
                     /locus_tag="VFI43_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7062571.1"
                     /translation="MNQQSVAEKVIIVTGAGQGLGAAIAATLGAAGAVIYACDIKEDQ
                     VKNVAQAINDSGGTAFPLVLDVRDQDAVRQAVAGIMEEQGQIDVLINNAGTDMTLSIE
                     DLSISDWERVVDVNLRGPFLLSKIVYPHLKRQGKGHIVNISSTAAKRAWTEATAYHAS
                     KWGLLGFSHALHAEARRDNIKVTAVVAGGMRTPFILERFPQTDPNVLQDPKNVAETVH
                     FVLTQPDETVIPEVMVLPMRETSWP"
     gene            complement(2317..4254)
                     /locus_tag="VFI43_10380"
     CDS             complement(2317..4254)
                     /locus_tag="VFI43_10380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7062572.1"
                     /translation="MTTRIALISEHASPLAAIGGKDTGGQNIAVAELARHLAALGYEI
                     DVFTRWDDRRVPKVLNWRGGIRIIHVEAGPVTFIPKEKLLPYMPAFTSDMLRFINGEN
                     ACYKLIHAHFFMSALVAADIKQKLGIPFIVTFHALAKVRRIHQGKDDWFSDEGFAVEE
                     RVLEEADQIVALCPQERDELISLYQADPRKITVIPNGFRPDEIFPLDKLFARMALKLD
                     PREKIILQLGRMVPRKGIDNVIKALGYISREHNVKVRLLIVGGESDEPDPKVTPEIGR
                     LQQLGETEGVAGQVTFVGRRTRDMLHYYYSAADIFITTPWYEPFGITPLESMACGTPV
                     IGSDVGGIKSTVLHGKTGFLVPPNDFALLGERIIELLSSTKLMMFFKENAIRHVNENY
                     TWMKAAHLTANLYERVATRSPLRANDEDGSLAFIDDSFESLIKTIRKSKRQIRLATLD
                     SAQAIYRSLARGGKVLVCGNGGSAAEAQHFSSELVGRFEANGRRGLAVMALTADTTFV
                     TAWANDFSFEQIFARQVEAHGQPGDVLIVISSSGQSPNLVNAVRAARKREMFCIGLLG
                     REGGPTGELTDINIIIPSHETTRIQEMQLHVLHVLSHLVEQQIMIDDRKSARMQEEWS
                     MVHFKVPGLNRKIATPEENLS"
BASE COUNT         1142 a         1447 c         1335 g         1239 t
ORIGIN      
        1 ttctgggtac aggtagcggt gccggatttt tgcaggatga ttcccgcagc ggatcttgca
       61 atctctcccg cgtcttctat tgccgcgccg cttgcgagcg cgagcgtgag ggccgcaaca
      121 tacgtatcgc ccgcgccggc agccttggtg ttctcgactg gttttgagaa ggttcgatac
      181 ggttccttgc cgcgctgaaa caacaacgcc ccctctatat cgatcgtggc cgccacatac
      241 tttgcgcccg tcttcctgag aagccgctgc ccgtacaacc tgatctgatt cgcgcgatgg
      301 cctagcgggg caggctcggt gatggaaagc agcgtgacaa gttcctggta gttcggcttc
      361 gacaccgtag ccccgacccg gctatattta tccagcgatt tcgcgtcgat caccaaaatg
      421 ttctcttttt ttctctgcaa aacccctaac gtcccgatga ccttgtccgt catgattcca
      481 tagccataat cagagacaac gaccgcatcg acctcgtgga atttttcttg caggatatcg
      541 attatccggc tctcgtattc catgcgtatt gcctccgtcg tccccgcatc gaaacgcacg
      601 agtaattggc tgcccgctgc cacccgctgt ttcgtaagcg tcctgcggac actgtcgcgg
      661 attacgaatg agagatcgag cccgcgacga gcgagcgcgt cttgaagcaa tcctgcctcg
      721 tgatcatcac ccacgacaga aagatagcaa acctccgccc cgaggcacgc caggttgaca
      781 gcggtattgg ccgctccgcc cggcattgtt ttgacattcc tgatatcgac gatgggaacc
      841 ggagcttccc ggcagatacg gttagcagag ccgtcaagat aaacatcgag catcgcatcg
      901 ccgatgacga gtaccttcaa atcgctaaaa cggttaatag tggcaagata atttctgttc
      961 atgctgcccc cttaccccgg aatcattccc tatgaagtcg ccacgaagag agctttcaac
     1021 tgccccacat gagccagggt cggacaaaca atgctggcgg cttcaagcca gtcgtcgacg
     1081 gtatgcaatg gtcgcctgtg attcccctcg tgccactcgg tttcgttgcc attgttcaaa
     1141 tgaattgtgc ggcagcctgc gcgattgccc gcctcgacgt cattcaagat atcgccaatc
     1201 atccaagaaa tttccaggtc gatcccatgg tcccgcgcgg cttgcaaaat catgcctggc
     1261 ttgggcttgc ggcattcgca ataatccgcg gggctatggg gacaataata gaaagcgtca
     1321 attttcacgc ggtatggcgc cagcagggcc tggattcgca gattgacggg catgaggtcc
     1381 tgttcctcaa acaagcccct tgcgattcct gactgattgg aaatgacaat cagtttatag
     1441 ccctctgccc tcagacgctg taatgccgtt cccgctccta ccgtgaggcg aacgtgctgc
     1501 ggatcgacgt tatagggcac atccggtata agagttccgt ccttgtcgat aaaaacagcc
     1561 ttatccaggc ccgggtgctt catcgcttct agggccacga tgtttccctc atcggcagca
     1621 ccatgacctc cgggattact gtttcgtccg gttgggtcag gacaaagtgg actgtttcgg
     1681 ctacgttttt gggatcctgg agaacattcg gatccgtttg gggaaaacgc tccaggataa
     1741 agggagtgcg catcccgccc gctaccaccg cagtcacctt tatattatcc cgacgagcct
     1801 cggcatgcag tgcgtggctg aaaccaagca atccccactt gcttgcgtga tacgcggtcg
     1861 cctccgtcca ggcgcgcttt gcagccgtgg aggagatatt gacgatatgt cctttgcctt
     1921 gacgctttag atgtgggtag acgatttttg acagaaggaa aggtcctctc aaattgacgt
     1981 ccaccacgcg ttcccaatcc gagatgctca aatcttcgat tgaaagcgtc atatccgtgc
     2041 cggcattgtt gatcaatacg tcgatttgcc cctgctcctc catgattccg gcgaccgcct
     2101 gccttacagc gtcctggtcg cgcacgtcca gcacgagcgg gaacgctgtc ccgccagagt
     2161 cattaatggc ttgagccaca ttttttacct gatcttcctt gatatcgcag gcataaataa
     2221 cagcgcccgc tgcccctagc gtagcggcga tagccgcacc cagcccctga cccgcgcccg
     2281 taacgataat gaccttttca gccacacttt gttgattcat gaaagatttt cctctggagt
     2341 agcaattttt ctgtttaaac cgggtacctt aaaatgaacc attgaccatt cttcctgcat
     2401 ccgggcagat ttgcggtcat cgatcatgat ttgctgctcg acgaggtggc ttagcacatg
     2461 cagaacgtgc agttgcattt cctgaatgcg ggtggtctcg tgagaaggaa tgataatgtt
     2521 gatatcagtc agttcacccg tcggaccgcc ctccctgccg agcaggccga tgcagaacat
     2581 ttcccttttg cgtgccgcgc gcactgcgtt gacgagattg ggagactggc cgctggaact
     2641 gatcacgatc aggacatcgc cgggttgccc gtgcgcctcg acctgccgcg cgaatatctg
     2701 ttcaaagctg aaatcgttcg cccaagcggt tacgaatgtt gtgtcggcgg taagtgccat
     2761 gaccgccaac ccccgccgcc cattggcctc gaaccgtccc accagttcgc ttgaaaaatg
     2821 ctgcgcttca gcagcactcc caccgtttcc acataccagt actttccccc cccgggcgag
     2881 agaccgataa atcgcctggg ccgaatcgag cgtcgccagc cggatttgac gcttcgactt
     2941 cctgattgtt ttgatcagcg attcgaaaga gtcatcgatg aaagccagcg agccatcctc
     3001 atcattcgcc cttagtggac ttcgggtcgc gacacgctca taaaggttcg ccgtaaggtg
     3061 ggctgctttc atccaggtgt aattttcatt gacatgcctg atggcatttt ccttgaagaa
     3121 catcatgagc ttcgtactgc tcagtaattc gatgatgcgc tcgccgagca gggcaaaatc
     3181 gttcggaggc acgagaaaac ctgtctttcc atggaggact gtggatttga tcccccccac
     3241 gtccgaaccg ataaccggcg tgccgcacgc catggattca agcggggtga ttccaaacgg
     3301 ctcataccag ggcgtcgtta taaatatgtc cgccgcactg tagtagtaat ggagcatgtc
     3361 acgggtgcgc ctgccgacaa atgtgacttg tccggctaca ccttccgtct cgccaagttg
     3421 ctgaaggcgg ccgatttcgg gcgtaacctt cggatcgggc tcatccgact cgccgcccac
     3481 gatcaacagc cggaccttga cgttatgctc ccggctgata tagccaagcg ctttgataac
     3541 attatcgatg cctttccgcg gcaccatccg gcctaactgc aggatgattt tttcgcgtgg
     3601 atccagtttc agcgccatcc gcgcaaacag cttgtccagt ggaaaaatct cgtcgggccg
     3661 gaaaccgttg ggaattacag ttatttttct tgggtccgct tggtaaagac tgatgagttc
     3721 atcccgctcc tgagggcata acgcgacaat ctgatcggcc tcttcaagaa cccgttcttc
     3781 cacggcgaag ccttcgtcag aaaaccagtc gtccttcccc tggtggatcc gacggacctt
     3841 cgcaagcgcg tgaaaggtca cgataaaagg aatgccaagc ttctgcttga tgtccgccgc
     3901 cacaagagcc gacatgaaga agtgcgcatg aatgagcttg tagcaggcgt tttccccgtt
     3961 gataaaacgc agcatgtcgc tcgtgaaagc gggcatgtag ggaagcagtt tctccttggg
     4021 aataaacgtt acggggcctg cttccacgtg aatgatgcgt atgcctcctc gccagttaag
     4081 gacttttggc acccgccgat cgtcccaccg cgtgaagacg tcgatctcgt accctaatgc
     4141 cgccaagtgc cgagccaatt cagcgacggc gatgttctga ccgccggtgt ccttgcctcc
     4201 aatagctgcc agcggagaag cgtgctcgct tataagggct attctcgtcg tcatacgcct
     4261 cccatatttc cggcccagcc ggggttgtgt gggttccatg tggaaaagaa gcgggttgaa
     4321 caccggcggc tatcatcaat agcggctgag gtattagtag cgatacgaag aggaaaaacg
     4381 cagggaaatg ctaaagaaac agcatgacaa tttgcatttt gcagttcgat cgactcgcca
     4441 gatcgcaagc tgatcatagg gaggtgctca acgagttgcc acaggcccgg atattattca
     4501 ttttcattac gagcttaaag catcaatgtg gctcatcgct ggcaggaaac ctttgcctgt
     4561 attgcttgta ttaaacattc gatttcgccg gttgttagag tcatgtaata actggcttct
     4621 gatcatttcg ccccgcataa aagctgaggt caaaaacgag ttcattttta atctgggggc
     4681 tcatactgtg tctgtgcggc gtcgcacgga actttaggcg aaggctgaga gccccgtcca
     4741 gctcgcgcgg gcgcttgcta ttctgttcgc ccgaagacga tattcagaga agggggaata
     4801 aagaagagga tggagggaat acgacaggta acagctcggc taacaaaggc gcgaatcgat
     4861 gcggcatcct gcctgagtta accccattcg ctttattcat gttgagtgtt gtgaagctat
     4921 ggcggatgaa aatcgcccct gattcgagaa aaatgtattg aacttctcaa gcataaatgc
     4981 ttcggctggc atcgcagggg ggcagcgttg tagcgctctt ctccataaaa cgttcgtttg
     5041 tgcaacttgc cacagcacct aacgcgagat gccaggtagg cacaagaaaa agcaatcagg
     5101 gcatccctct gctacatcgc tcagaatatc gttctatcga ttatgatatt tgtcgggtat
     5161 tgt
//
LOCUS       DASSHJ010000271         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_450952, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000271 DASSHJ010000000
VERSION     DASSHJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450952"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFI43_10385"
     CDS             <1..895
                     /locus_tag="VFI43_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7062573.1"
                     /translation="AGVMGNAEIRAHGRSRRVTVYGEGPDFTRVFNMKISIGQFLPPD
                     DPRSPRPYAVLGSRVRTELFGNTNPLGQTVQVGTTRFRIIGVMESKGQVLGFDFDDTV
                     FIPTSRALEIFNREGVMEIHISYPPSAPLQVVVDETRQILVSRHGREDFTIKPQQQML
                     KTLGTVLDVLIFAVAALGGISLLVGAVGMVTLMHIAVAERVAEIGLLTALGATRARIR
                     ILFLMESTLLSTLGGLGGLVIGVGIAWLLKSVQTALPVHTPWNYAFGALGISVAIGLA
                     AGVAPAMRAARLDPIEALRAE"
     gene            1265..1714
                     /locus_tag="VFI43_10390"
     CDS             1265..1714
                     /locus_tag="VFI43_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062574.1"
                     /translation="MRKNFEAMRCHRTYLRAKTALRDQHQDAYVLMLERHAQELDTLW
                     ADYNLAVKKLAEEHNKAVKSRILAIKKAGKKAAAQKPEEKSRLQTAADTGFKVLPMKK
                     RVKMESNMTAEERGGQDNELINGLPQVSDSAPERNALLPKFFGFMRL"
     gene            complement(1851..2198)
                     /locus_tag="VFI43_10395"
     CDS             complement(1851..2198)
                     /locus_tag="VFI43_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062575.1"
                     /translation="MANRLEAEISSVIFELKNLQPIFRQDGRVTLFAVVLTLFVVSMA
                     YWLWGAYAQVSLEMNLACRNPAAEACVSAKARYMNSFFAAWLSQMFPWILPFVPAFIY
                     VFFFRWLRRNRQP"
     gene            complement(2282..>3197)
                     /locus_tag="VFI43_10400"
     CDS             complement(2282..>3197)
                     /locus_tag="VFI43_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062576.1"
                     /translation="RNMARLADQGHSRGEIEAMFAGYSEPPPVNWWILGGGLAFTLAS
                     LSVGLSGIPGGEEIIFAVSMAIVVFLMWRLTGELEPEPRNVLVGTAFMIFVFRAIPGP
                     GPGSTWWMIDDLGFDQQFLAALSLIGATLTLAGMFIFRRFMAERSIAYIIGMLTIVGT
                     LLSLPTVGMYYGLHHWTAAHTGGVVDARFIAVIDTALESPLGQIAMIPMLAWIANSAP
                     ERLKATFFAVMASFTNLALSASQLGTKYMNEIFTVTREVKDPATGQIAVPADYSELGM
                     LLISVTVIGLVLPLAAILLVRKTRFRNA"
BASE COUNT          791 a          877 c          849 g          680 t
ORIGIN      
        1 cgcaggcgtg atgggcaacg ctgaaattcg agcgcacggg cgaagccggc gcgttacagt
       61 ttacggggaa ggtccggatt tcacccgagt cttcaatatg aagatatcca ttggccagtt
      121 tctgcccccc gatgatcctc gcagtccacg cccctatgcc gtacttggat ccagggtgcg
      181 cacggaactc ttcgggaaca cgaatccctt gggtcaaact gtgcaggtag gcacgacgcg
      241 ctttcggata atcggcgtca tggaatccaa gggccaggtg ctgggctttg atttcgacga
      301 taccgtattc atccctacct cacgcgcact cgaaattttc aaccgcgaag gtgtcatgga
      361 aatccatatc tcataccctc cgagcgctcc acttcaggta gtggttgatg aaacccgtca
      421 gattcttgtc tcgagacatg gacgtgagga ttttacgatc aagccgcaac agcagatgct
      481 gaaaaccttg ggaaccgtgc tcgatgtgct gatcttcgcg gtcgcagccc tgggcggcat
      541 atcccttttg gtcggcgcgg tcggcatggt caccctcatg catatcgcag tcgctgagcg
      601 cgtcgccgaa ataggcctgc ttactgcact tggtgcaacg cgggcccgca ttcgtatcct
      661 gttcctgatg gaatccactc ttctttccac gctaggggga ctgggcggac tggttatcgg
      721 tgtaggaatc gcatggctgc tgaaatcggt gcaaacagcc ttgcccgtcc acacgccctg
      781 gaattatgcg ttcggagcat tggggatttc agtggcaatt ggccttgccg ctggtgttgc
      841 ccctgccatg cgcgcggccc ggctggatcc catcgaagca ctccgggcag aatgacagaa
      901 catttatgac ctgaaatcac tgacattaaa acgagccgtc actcgtgtct atccagtcag
      961 cgattgcgga ggctcccctt cacgcacgta cctatacgta gccggatatc tgtaataaac
     1021 attcctcaag gccgaactgt tcgtcacggt tcactcccct ctgaacattt tcaaatctgc
     1081 actgttgcgt ggcgagcgat gggacgcctt ggcccaaggt gtactaaagt ctactgtacc
     1141 aaagtccaat tgttctcttc ttcaaagttg atacgatgcc ggacgtcgca caaccaatgc
     1201 ttaatctcaa tgcttgtttt aacgttgaat cgaaccccca tcaattcact acccaatctg
     1261 agtcatgaga aaaaattttg aagcaatgcg ttgccacagg acttatcttc gcgcaaagac
     1321 cgctctgcgc gatcagcatc aggatgcata cgtcttgatg ctcgaacgcc acgcccagga
     1381 actcgatacg ctctgggcgg actacaacct cgcggtgaaa aagttggccg aggaacataa
     1441 caaggctgtc aagtcccgga ttctggcaat caagaaggcc gggaagaagg cagccgcgca
     1501 aaagccagag gaaaaatccc gcttgcaaac ggctgcagat acaggcttta aagtcttgcc
     1561 gatgaagaaa agagtaaaaa tggaatcaaa tatgacggct gaggagcgcg gcgggcaaga
     1621 taatgaatta attaatggcc ttccccaggt atctgatagc gcccctgaaa gaaacgcgct
     1681 actgccgaag tttttcggtt ttatgcggct ttaaaaggca aaagaccgaa ctggctccag
     1741 aaacggtatg cttggttgta ccattgtatt tccgggatat ttccgggaat ctttcctgcc
     1801 gatccaaggc ggcgaagcaa atcgtcgcca gccttgcaat ggtagaagcg tcaaggctgg
     1861 cgatttcggc gcagccagcg gaaaaagaat acgtagataa aagccggaac gaacggcagt
     1921 atccagggaa acatttgcga aagccatgcc gcaaaaaaac tgttcatgta gcgcgctttg
     1981 gccgacacgc acgcctctgc ggcgggattg cgacatgcaa ggttcatttc cagcgacacc
     2041 tgggcataag cgccccaaag ccaatatgcc atggaaacca cgaacagggt cagtaccacc
     2101 gcgaagaggg tgactctgcc atcctgccgg aatatgggtt gcaggttctt caactcaaat
     2161 attaccgagg aaatttcagc ctcgagccgg tttgccaaac aaaggagatc attgcctgat
     2221 ttcttaagcc aggcatgatt ttgcgagccc gatattcccg aggctatcat aaaagcactt
     2281 cttatgcatt tcggaaacgt gttttcctga cgagcaggat ggcggcaagc ggcagcacga
     2341 ggccgatcac ggtgaccgag atgagaagca tgccaagctc gctgtaatcg gcaggaaccg
     2401 ctatctgccc tgttgcggga tccttcactt cccgtgtaac ggtaaatatc tcgttcatgt
     2461 atttcgttcc aagctgggaa gcagacagcg cgagatttgt gaaggaagcc atgacggcaa
     2521 agaacgttgc cttgagtctc tcaggcgcag agttggcaat ccaggccagc atcggaatca
     2581 tcgcgatctg ccccagaggt gattcgagcg cggtatcgat cacggcaatg aagcgagcat
     2641 ccaccactcc ccccgtgtgt gccgctgtcc agtgatgcag cccgtaatac atgccgacgg
     2701 tgggcagcga taacagcgta cccacgatgg tgagcatgcc gatgatatag gcgatcgaac
     2761 gctctgccat gaacctgcga aaaatgaaca tgcccgccag ggtaagcgtt gcaccgatca
     2821 gcgacagggc ggccagaaac tgctgatcaa accccaggtc gtcgatcatc caccaggtgg
     2881 aaccggggcc cggacctgga attgcgcgaa agacgaagat catgaaggcc gtgcctacca
     2941 ggacgtttcg aggctcgggt tcgagttctc cggttaaccg ccacatcaga aacacgacaa
     3001 ttgccatcga tacggcaaag atgatttctt ctccgcccgg tatcccgctc agaccaacac
     3061 tcagggaagc cagggtaaaa gcaagcccgc cgcccagtat ccaccagttg accggaggag
     3121 gctcgctata gccggcaaac atcgcttcga tttcgccgcg gctatgcccc tggtcagcca
     3181 agcgtgccat attccgc
//
LOCUS       DASSHJ010000272         6591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1345424,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000272 DASSHJ010000000
VERSION     DASSHJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345424"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /gene="rnhA"
                     /locus_tag="VFI43_10405"
     CDS             <1..481
                     /gene="rnhA"
                     /locus_tag="VFI43_10405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET7062577.1"
                     /translation="DRVVEIFTDGACKGNPGIGGWGALLQFNGHTRELFGGEKLTTNN
                     RMELLAVIRALEALKEPCRVRVHTDSTYVQKGISEWIHAWKGRGWRTADKKPVKNEDL
                     WKELDTLSQRHSIEWLWVRGHSGHDGNERADKLANMGAQTMQAGSVNNSHAVPDKPA"
     gene            648..1355
                     /gene="dnaQ"
                     /locus_tag="VFI43_10410"
     CDS             648..1355
                     /gene="dnaQ"
                     /locus_tag="VFI43_10410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HET7062578.1"
                     /translation="MRQIFIDTETTGLDFKLGHRIIEIAAVEMRSRRLTGQRFHCYLN
                     PERDIEDGALQVHGLTLEFLQDKRKFREIGRELLEFVEGAELIIHNAAFDVPFIDHEL
                     SLAQLSQLSQCCHLITDTLIMAKELYPGKKNNLDALCERYEIDHSHRSLHGALLDAQL
                     LAEVYLAMTRGQDSLLIDLEPLAELDFAATLDSLELIILAPTEEELEAHAQQLESIER
                     ESKGKCLWKRLEAAAEV"
     gene            complement(1488..4541)
                     /gene="putA"
                     /locus_tag="VFI43_10415"
     CDS             complement(1488..4541)
                     /gene="putA"
                     /locus_tag="VFI43_10415"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HET7062579.1"
                     /translation="MNPCFAADHLADEAACVENLLSSLEWNDVRAKRIRQQAERLIHG
                     IHGHSDALGDIDAFLGHYPLDTPEGLALMSLAEALLRISDADTADALIAEKLGTASWK
                     AEEGGGMMRLADAGINLARKMLRTSPGRFERSLIRKSMEEAVRRIGHQFVLGENMADA
                     LSAGKKLEHRGYGISYDMLGEGARTAADAQRYFEAYADGVDALGKSKHSPREKGISVK
                     LSALHPRYTWNQKDKCVPEIARRLTELCIRAAESNISLTVDAEEADRLEISWEIISMV
                     GALPELRGWNGFGLAIQAYDKRCFRLIEDIGRLAANTSRKLCVRLVKGAYWDTEIKRA
                     QVLGLADYPVFTRKANTDLSYLAGAQKLLGLRRLVYPMFGTHNAYTIAAILDMAGNSG
                     GFEFQRLHGMGDALYEQVLGEYKIPVTAYAPVGNYEELLPYLIRRLLENGANTSFVRQ
                     VRGGSVENVLRDPVEEAGQAQSKRNLRIPLPSNLYGVERANSAGPDLSAETMRTGFLE
                     RIPSRNTWRNMAVLPIINGRVADREATTQGLRSHPSPGNRNEIITRIGEASADNLEDA
                     FLAARQGIHEWSRVPSFQRAEVLRGVANLIQDEMPRFVGLLQFEGGKTLQDAISEVRE
                     TIDFCRYYAMQGERLFDERGVALPGPTGEENRVVMEARGIFLCISPWNFPLSIFMGQI
                     VAALMAGNSVIAKPAEQTPLIAFEAIRALLDAGVPPAAVTLLPGGGETGAKLVEHPDV
                     SGIVFTGSVEVGHLINRALAAKDGPIVPLIAETGGQNAMIVDSSALPEQVIDDVLLSA
                     FGSAGQRCSALRVLYLQEEISARILELLRGAMLELRIGDPSRFSTDIGPVIDADAFHS
                     LSRHLRNLKEYGALIGQAPLPEGLDGWYIAPAAYEIDSIFRLPGEVFGPILHVIRYEA
                     KNLARVIDDINATGFGLTFGMHSRLQHRTTQVAGQINAGNVYINRSMIGAVVGVQPFG
                     GHGLSGTGPKAGGPYYLQRFANEKAIAVNTAAIGGNIALLNAGAE"
     gene            complement(4571..5065)
                     /locus_tag="VFI43_10420"
     CDS             complement(4571..5065)
                     /locus_tag="VFI43_10420"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HET7062580.1"
                     /translation="MVGRIDSKPIRLRGKREEVPERYCALQLEEIEKKNMHFLLILGI
                     IVAIAAVAFALQNNTTVTVAFGAWSFDSSLAMVLLLALGVGAVIAISLSWPGFIKNLW
                     ASSRLRRQVNQLQDDKAMLEQRVAQLEEELRRVSPEPVPAEPPRYLGLKSILIGEKAE
                     TPRE"
     gene            complement(5203..6402)
                     /locus_tag="VFI43_10425"
     CDS             complement(5203..6402)
                     /locus_tag="VFI43_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HET7062581.1"
                     /translation="MEIATKIKKGTLLAYVRNALAVPLAGLATFFYIAWQLLIEQQLF
                     LRTFLMQRMGKRGIPILPLGYRNVLTLKSIMLWQHLSGNPDAFKAARRHTEQRILQRL
                     QARDLPRRRTLSIKEYRPGEIDPSRFYREHVKRSVPCVLRGFIQNAPHDWTLAKLAER
                     FPDTVVQALDKRTKKMINTSLRHIAEDRRHNLIPQQLLLDQNPVFYRYFDIPRSHAYF
                     PVLGRPSKPLLSFLILGLGAGLNANYHCEEGPNWYMAVSGSKRWTLIESEYSWLLYPA
                     AIGNGMRRFAEFRANEQGEPSDREAYPLVEYAPRYEFDLHPGDVLFFPAWMWHKTINL
                     NEEGLGITCRYTAPGKISNRYFRGLQLLSSGFWKSCIEVISCSIRGNIADLAADTGHN
                     EQETGLY"
BASE COUNT         1448 a         1864 c         1783 g         1496 t
ORIGIN      
        1 cgatcgagtc gtggagatat ttaccgatgg ggcctgcaag ggtaatccgg gcatcggggg
       61 atggggtgcg ctgctccagt ttaatggaca cactcgtgaa ttgttcggtg gggagaaatt
      121 gaccaccaac aaccggatgg aactgctcgc ggtcattcgc gcgctcgaag ccctgaagga
      181 accttgccgt gtgcgcgtgc ataccgattc cacttatgtt cagaagggca tcagtgaatg
      241 gatacacgcc tggaaggggc gggggtggcg cacggcagac aagaaaccgg taaaaaatga
      301 ggacttgtgg aaagaactcg atacgctgtc gcagcgccac tccatcgagt ggttatgggt
      361 gcgcggtcat tccggtcatg atggcaatga gcgtgccgac aagctcgcca acatgggcgc
      421 gcaaacgatg caggctggct cagtgaataa ttctcatgct gtgccggaca agccggcttg
      481 aatgtgtaca gcgtccggga tcccaaatga cgttcccgat tgcccgatgc cctattttga
      541 acgactgata tcatgatggc gattgatcta tcttccgcaa atttggctaa tatcatatcc
      601 gtaccttacg tcaggggtac tgacccatca aaaataagcg tcccggtatg cgccagatat
      661 tcatagacac tgaaacgacc ggtcttgact tcaagctcgg tcatagaatt atcgagattg
      721 cggcggtgga aatgcgaagc cggaggctca ccggccagcg ttttcattgt tacctcaacc
      781 ccgagcgcga catcgaggat ggcgcgttgc aggttcacgg cctgaccctg gaatttctcc
      841 aggacaagcg caaattccgc gaaattggcc gggagttgct cgaattcgtc gagggagccg
      901 agttgatcat acacaatgcc gctttcgacg tgccattcat cgaccatgaa ttgagtcttg
      961 cacagctttc ccagttaagc caatgctgcc atttgatcac cgatacacta ataatggcga
     1021 aggagctata tcccggcaag aagaacaacc tggacgcttt atgcgaacgc tacgaaatcg
     1081 atcattcgca ccgatccctg catggcgcgc tgctcgatgc ccagttgctg gcggaagtgt
     1141 atctggccat gacgcgggga caggacagcc tcctgatcga tctcgaaccg ctcgccgaac
     1201 ttgatttcgc ggcgacgctg gatagtcttg agctgattat cctggccccg actgaggagg
     1261 aactcgaagc ccacgcgcag cagctcgaaa gcatcgagcg cgagagcaag ggcaagtgct
     1321 tatggaagcg gctcgaggcg gcggcagagg tatagcagta gcagccgcaa catcgcacac
     1381 tatgcaagct gatgaaggag agcctccgcg cgggcaaccc tggctttttt ctcccacgct
     1441 tttttctccc gtaaacgtgg agagcgggac ttgccggcca tgatttccta ttccgcccct
     1501 gcatttaaaa gcgcgatatt tcctcctatg gcggcggtat tgaccgctat agccttttca
     1561 tttgcaaagc gctgcaggta atacggtcct cccgctttcg gaccggttcc tgacaagcca
     1621 tggcccccga atggctgaac acctaccacg gctccgatca tgctgcgatt gatgtagaca
     1681 ttgccggcgt tgatctgccc cgcgacctgc gtcgttctat gctgcagccg gctgtgcatg
     1741 ccgaatgtca ggccgaagcc agttgcgttg atatcatcga tgactctggc gaggttcttt
     1801 gcttcatagc gaatgacgtg caggatcggg ccgaacactt caccgggaag gcggaaaatg
     1861 ctgtcaatct cgtacgcggc aggtgcgata taccatccgt ccaatccttc gggcaggggt
     1921 gcctggccga ttaacgcgcc atattccttg aggtttcgca gatgtctgga aagggaatga
     1981 aaggcatctg catcgatgac cggaccgata tccgttgaaa aacggctggg atcgccaatg
     2041 cgcagttcca gcatcgcgcc tctcagcagt tctaggatcc tggcggagat ttcttcctgt
     2101 agataaagca cccgcaaagc ggagcagcgc tgtccggcgg aaccaaaggc gctcagcaat
     2161 acatcgtcga ttacctgttc cggcaacgcg ctcgaatcca cgatcattgc gttctgtccg
     2221 ccagtttccg caatcagcgg aacgatgggg ccatcctttg cagccagggc ccgattgatg
     2281 aggtgcccca cctcgaccga tccggtgaaa acgataccac ttacgtccgg gtgttccaca
     2341 agcttcgcac ctgtctcccc gcctccggga agcagggtga ccgcggcagg cgggaccccc
     2401 gcatcaagta gcgcgcgtat cgcttcgaat gcaatgaggg gtgtctgctc cgccggtttg
     2461 gcaatgacgc tgttccctgc cataagggca gccacgatct ggcccatgaa tatggatagg
     2521 ggaaaattcc agggactgat gcagagaaat attccgcgcg cctccatcac cacgcggttt
     2581 tcctctcctg tggggccggg aagcgcgact ccacgttcat cgaacaggcg ttctccctgc
     2641 atagcatagt atcggcagaa atcgatcgtt tcgcgcactt cgctgatggc atcctggagc
     2701 gtttttccgc cctcgaactg caacaatccg acaaatcgag gcatctcgtc ctgaatgagg
     2761 ttcgccaccc cgcgcagcac ttccgcgcgc tggaaggagg gcaccctcga ccactcatgg
     2821 atcccttggc gagcggcaag gaatgcatcc tccaggttat cagcgctggc ttcccctatc
     2881 cgggtgatga tttcgtttcg attgcctggg ctcgggtgcg agcggagtcc ttgcgttgtt
     2941 gcttcccgat cagccacccg gccgttgatg atcggcagca cagccatatt gcgccaggtg
     3001 ttccgggagg gaatacgttc cagaaaacct gttctcatcg tttcagcgga taaatccggg
     3061 cccgctgaat tcgcccgttc cacgccataa agattcgatg gcaggggtat ccgcaggtta
     3121 cgcttagact gggcttggcc cgcttcctca accgggtctc ttaacacgtt ctcaaccgag
     3181 cctcccctga cttggcgaac gaatgatgta ttcgcaccgt tttccagcag ccgccggatc
     3241 aggtaaggca agagttcttc atagttgccc acgggtgcgt aggcggttac gggaatcttg
     3301 tattccccaa gcacctgctc atacaacgcg tcacccatgc cgtgaagccg ctggaattcg
     3361 aatcccccgg aatttcccgc catgtcaagg atggctgcaa tcgtgtacgc gttgtgcgtg
     3421 ccaaacatcg ggtaaaccag gcggcgcaat ccgagaagct tctgcgctcc cgccaggtat
     3481 gacagatcag tattggcttt acgcgtaaag accggataat cggccaaccc caatacctgg
     3541 gcccgcttta tttcggtatc ccaatacgcg cccttcacga ggcgcacgca cagctttcga
     3601 gatgtattgg cggccagacg gccaatgtcc tcgatcaggc gaaagcagcg tttgtcataa
     3661 gcttgtatag cgagcccaaa cccattccat ccgcgcaact cgggtagggc gccgaccatt
     3721 gaaatgatct cccaagagat ttcgaggcga tccgcctcct cggcatcaac ggtgagcgat
     3781 atgttgcttt ccgcggctct gatgcaaagc tcagtcagcc tcctagcgat ctccggaacg
     3841 catttgtcct tctgattcca ggtatagcgg ggatggagcg cggaaagctt gaccgaaatt
     3901 cccttttcgc ggggagaatg ctttgacttg cccagggcat ccacgccgtc ggcatacgct
     3961 tcaaaatacc gctgcgcatc ggcggcggtc cgcgcaccct cgccgagcat gtcgtaggat
     4021 atgccgtatc cgcggtgctc gagctttttg ccagcggaga gtgcgtcagc catattttct
     4081 cccagcacaa attggtgtcc aatacgtctt accgcttctt ccatgctttt acgaatcagc
     4141 gaacgttcaa agcggccggg agacgtcctg agcattttcc gggccaggtt gattcccgca
     4201 tccgcgagcc tcatcatccc gccgccttcc tcggctttcc agcttgccgt tcccaatttc
     4261 tccgcaatca atgcgtcggc agtatccgca tcggagattc gcagtagcgc ttcggccaag
     4321 ctcatgagtg ccagcccttc cggagtgtca agggggtaat gcccgagaaa ggcgtcaata
     4381 tcgcccaggg cgtcgctatg gccgtggatg ccgtggatga gtcgttcggc ctgctgcctt
     4441 atccgtttgg cacgaacatc attccattcg agcgacgaga gcaggttttc aacgcaggca
     4501 gcctcatcgg caagatggtc ggctgcaaaa caggggttca tgggaagaca tgaagtaata
     4561 cggcgaggaa ttattctctg ggagtttcgg ccttttcgcc gataagtatg gatttcaggc
     4621 cgaggtaacg cggaggctcg gcggggacgg gttccgggct tactctacgc agttcctctt
     4681 caagctgtgc aacccgctgt tcgagcatgg ccttatcatc ctgcaactgg ttgacctgcc
     4741 ggcgcagccg cgagcttgcc cataaattct tgatgaaccc gggccatgaa agggaaatgg
     4801 cgataaccgc ccctacaccc agcgcgagca gaagaaccat ggcaagcgaa ctgtcaaagc
     4861 tccatgcccc gaacgcaacc gtcacggtcg tattgttctg cagcgcaaaa gccaccgctg
     4921 ctatcgcgac aataatcccg agtatcaaca gaaagtgcat attctttttc tcaatttcct
     4981 ccagctgcag tgcgcaatat cgttcaggca cctcttcccg ttttccccgg agcctgatcg
     5041 gtttactgtc gatcctgccg accatcagtc acctgcacag atagattcac agtacgctcg
     5101 ccggttcttc ccgtgaaggg tgacctgtta cgcgggcgca gacatgatgg ccttcttcgc
     5161 cggcctgaga aaacgaggga gctgatcggc tttcttttta ttttagtaca gcccggtttc
     5221 ctgctcgttg tggccggtgt cggcagccag atcagcgata ttgccccgga tgctgcaact
     5281 gatgacttca atgcaacttt tccagaaacc ggacgaaagc agttgcaggc cgcggaaata
     5341 cctgtttgaa atcttgccag gcgccgtgta tcggcaagtg attccaagtc cttcctcatt
     5401 aagattgatt gtcttgtgcc acatccaggc ggggaaaaac aaaacatccc ctggatggag
     5461 gtcgaactca tagcgcggcg cgtattccac taatggatac gcctcacgat cactcggttc
     5521 gccctgttca ttcgcgcgaa attccgcgaa ccgtcgcatg ccatttccga ttgcggcggg
     5581 atatagcagc caggaatact cggattcaat caaggtccag cgtttggaac ccgaaacggc
     5641 catgtaccag tttggacctt cctcacagtg ataattggcg tttaatcctg cgccgagacc
     5701 gagaatcagg aagcttagaa gcggtttgga gggacgtccg aggaccggga aataggcgtg
     5761 tgagcgtggg atatcaaagt accggtagaa aaccggattt tgatcgagca gcagttgctg
     5821 aggaatcaaa ttatgccggc gatcctcggc aatatgacga agtgatgtgt tgatcatctt
     5881 ctttgtacgc ttgtcgagcg cttgcacgac agtgtccggg aatcgctccg cgagctttgc
     5941 aagcgtccag tcatgcgggg cgttttgtat aaatccacgc aacacgcaag gaaccgaccg
     6001 tttgacatgc tcccgataaa accgcgacgg atcgatctca ccgggccgat attcctttat
     6061 gctgagggtt ctcctgcgcg gtaagtcgcg ggcttgcaat ctctgcaata tccgctgttc
     6121 cgtatgccta cgcgccgctt tgaacgcgtc aggattaccg gataagtgct gccacagcat
     6181 gatgctcttt agagtgagca cgttcctgta tcccaggggc aatatcggta tccctcgttt
     6241 gcccattctc tgcatgagaa acgtacgcag gaaaagctgc tgctctatga gcaattgcca
     6301 cgctatataa aaaaatgtag ccagtccggc caggggaacg gccaaggcat ttctcacgta
     6361 tgccagcaaa gttccttttt ttattttcgt cgcaatctcc atctaatgct cctttggttt
     6421 ccttctcgac attcgatttc atcctgaaag caatacaccc agaacttgtc agctattggt
     6481 caatactcag gcctctggat gacgtgatgg ataatacaac taactccgcc gttcaacggc
     6541 aatcccatgt ggaggacctg ggctggtcgg gatagtcgta aacccctttt c
//
LOCUS       DASSHJ010000273         8364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1477808,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000273 DASSHJ010000000
VERSION     DASSHJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477808"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFI43_10430"
     CDS             complement(<1..645)
                     /locus_tag="VFI43_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062582.1"
                     /translation="MEGSKHADAGRDARLSASRPPGVYYELFFEKKVQPAFQTGVPVF
                     VGFAKLRNQERQHATRIHHLDRWPQFQRFETASQNYLSYAVRGFFENGGEHCVVIPVE
                     SMQKGDAAEVLTNIFRPGGVLDDLEDIDLVCIPDAMAPDIRSNYQAVQDIQAGALEHC
                     RRMGDRFAILDGFRGREDDNRKPSAQKGALTGSAISHWQALPPECGALYYPWVSV"
     gene            complement(678..2321)
                     /locus_tag="VFI43_10435"
     CDS             complement(678..2321)
                     /locus_tag="VFI43_10435"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HET7062583.1"
                     /translation="MHMTRLTYQAPGVYVEEIPSARQPIAGVGTNTAAFIGIVSDTIY
                     YPVPNPYYDPVAARAALAAPGGVPAAGAGGETPEAVRDRIRAERDKLKEELTALQNTE
                     GTPRNQIKDKQTAVATKETELENAETAVARAHAPAETEDDTAYEDGAPDADWMEPAPG
                     QQAPREHPYYLKAFNVEVDPWDTKFCTNFTEYTNRFGPFSAFRSGTESYRPMFPGHHA
                     LTHAVNGFFKNGGTRCFVARVRSAADIPNVLKNMESIDEVALIAAPGLPKRIDVWNPL
                     MDYAEDENHENVFAILDSPKVVGDPNNFDIQRITYTGSGEEPIPHPSKNAAFYFPQIE
                     VVDPAKQIQDTDPALRVEAKYRGRTFVAPSGHMAGVYARTDEERGVHKAPANCVVRGA
                     INVKYYVSKPNQEVLNPQGVNVIKTINGAVTVWGARTIGGDRNGEWKYINVRRFFLFL
                     QESIDEGTQWVVFEPNDYALWGKIRLNVTAFLTNVWRSGALFGLTPDEAFYVKCDAEL
                     NPPEVRDLGQVITEIGVAIVRPAEFVIFRISQSTGQEKT"
     gene            complement(2451..2867)
                     /locus_tag="VFI43_10440"
     CDS             complement(2451..2867)
                     /locus_tag="VFI43_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062584.1"
                     /translation="MKTWTDWEIVRHHVSICGRVVDSSGKPLHGIRLGLVPHISESEL
                     QPERPRSPRSRNTRATAQQEVENPGVRGWKKTESGFDGGFFFLDCPDGEYTLIARDTH
                     SGLEVEQTVHSHRDVLEKRVKDRKPDDGYQVELVLK"
     gene            complement(2864..3478)
                     /locus_tag="VFI43_10445"
     CDS             complement(2864..3478)
                     /locus_tag="VFI43_10445"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HET7062585.1"
                     /translation="MINDLDETLSKLLKQKLPRRLADQVQVSFSAPDDQFPPPSVTLP
                     AIDLFLYDIRENLDLRSNEIYFDRKSDGTTVQKQAPVRMDISYLITAWPSQSVTDPSE
                     DEHRLLGEVIRALIRYRTIPKEMLQGELVSQDLPLPVTTLQPGRLQSLGEFWQALGGK
                     PKAALNYTVTLAVQAADEKPVDLVTEKVLKFRTQMGATPEEAES"
     gene            4062..5669
                     /locus_tag="VFI43_10450"
     CDS             4062..5669
                     /locus_tag="VFI43_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7062586.1"
                     /translation="MRQSFHLCSPASFAFRNALGVRSTIARAGGTVCTTVTLITLCGF
                     SFAGQLQLMSVKETSPGAVTATVGVRTGGTPNASDFQLLLDGKNEIKAGEVKPASSSL
                     LETSVILCVDQSGSMGPARIKQLQDAIRSALVKPEGGRLSLALWAFDSDVTKLHDFSE
                     NAEELSKAAGKIGLRSARDNQTKLYEAIALGLSELRNYPKNGPKRLILMTDGKDDGSS
                     ITEQVVVNEANAQSVIIDAIGFGNVSDHDANLLARLAKSAGGYFTRVNSADQLSRELQ
                     KLLSLPPPRVFDVNFHYKASGEADRAKIAQLKFTAAGQPPILLNIQQTLSALQSVSSD
                     RKESQPDKEKNSDLSILLWIMVGLLAIVGVYLLGRSKSKEEVKHEITPEPTPAPPPAS
                     TEPEPVKRPRTMVAFMFPPPAKGQPAAYLDGISGKARGEQFPIEQKVTHIGAGDDNDL
                     RMDDEYVSRKHAAIRYDSGGLYLNDSGSRNGTFLNGAELHHSAMALAPGDQIRVGKTT
                     FRVRARDSQAPRRPDKGPGTVTEPTVP"
     gene            5708..6499
                     /locus_tag="VFI43_10455"
     CDS             5708..6499
                     /locus_tag="VFI43_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET7062587.1"
                     /translation="MEGSSTTALSVGARSETGYVRSENQDRMSRVEAPFGEIYIVSDG
                     MGGHRGGGIAAALTVEILGRMLSAIPAVSNAACSVKAAFEEANRDVYEKGHHGDEQTR
                     GMGATAVVLAIGGPQALVAHVGDSRAYLFTHRELRRLTKDHSRVQRMVDAGILTDAEA
                     SSHPSASLLERAIGVLPDVEVDISPPIDLNGGELFLLCSDGLHGYVSDREIGAILSRR
                     IPVQAMADELVDAALEKGGEDNITVQLVGYELAERSDQTPIAGIP"
     gene            6649..7389
                     /locus_tag="VFI43_10460"
     CDS             6649..7389
                     /locus_tag="VFI43_10460"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7062588.1"
                     /translation="MSEKPKSGVFSQLLKHIIPGEKSSTRTRYCEKGHPMDANWTTCP
                     YCEAEKRKNDKSIIRTTTKSDQIDKGETPQRSADMERGTTKIGTGSPEEPDGRTRFDT
                     SPEQVSEKKVVGRERRITGVLVTFSHKYQGELFVLYEGRNVMGSGSSCDISINWDRLV
                     SGEHAMILCRAGRDELHDMLSTNGTFLNEEYVGTKGADLADGAMIRIGSTVFEFRKIT
                     SGAGKGPGEKAEPSEEDSELRPPGETDF"
     gene            7416..>8364
                     /locus_tag="VFI43_10465"
     CDS             7416..>8364
                     /locus_tag="VFI43_10465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7062589.1"
                     /translation="MKYSGSSARAGEFREGQACGKYILESVIGRGGMGTVWRARNVTS
                     GQPVALKVVADDLLAEPHIRARFENEMRRHARLDHPNIVRLLDTFSVNGQQCMVMKLM
                     EGESLAVRLRRGPDHALPLDVSLQIFSGVLRALDYAHRHGIFHRDVKPSNILVDAANE
                     AYLLDFGIALAVGEARLTGMGVPVGTPAYMSPEQIRAPRQIDHRTDVYSAACVLYEML
                     TGRPPFLYLKVGGTTAEIALRWAHVNERPIPPHELMPSIPPGISRIIMMALNKDPNER
                     PQGCAEFLRLLLAEFADTGHVEAQKEAQRRWEEFVSDSTL"
BASE COUNT         1892 a         2232 c         2312 g         1928 t
ORIGIN      
        1 gacggaaacc caggggtaat aaagcgcgcc gcattccggc ggcagagcct gccagtggga
       61 tatggcggag ccggtaagcg cccctttttg cgctgatggc tttcggttgt cgtcttcccg
      121 accccggaaa ccatccagaa tcgcaaaccg gtcacccatc ctccgacaat gctccagagc
      181 gcccgcctgg atatcctgga ccgcctgata gttcgatcgg atgtcgggcg ccattgcatc
      241 ggggatgcac acgaggtcta tatcttcgag atcatccagc acaccgccag gcctgaagat
      301 atttgtaagc acctcggccg catcgccctt ttgcatgctt tcaaccggta ttaccacaca
      361 atgctcgccc ccattctcaa agaaacctct tactgcatag ctcagatagt tctgagaagc
      421 ggtttcaaaa cgctgaaact gcggccagcg atcgagatga tggattcgtg ttgcatgttg
      481 cctttcctgg tttcgcagct tggcaaatcc cacgaacacg ggaaccccgg tctgaaatgc
      541 cggctgaacc tttttctcga agaagagttc gtagtagacg cccggtggcc gggaagccga
      601 taaacgagcg tcccgaccgg catccgcgtg tttgctgcct tccatgatct aacctggata
      661 tccttttatt tgaatgatca ggttttctcc tggccggttg attgcgaaat gcgaaaaatg
      721 acgaactccg cgggccggac gatcgccacg ccaatctcgg taataacctg tcccagatcc
      781 cgtacttccg gaggattcaa ttcggcatca catttgacgt agaacgcttc atcaggggtc
      841 agcccgaaca gcgctccgct gcgccagaca ttggtgagga aagcggtgac attcaggcga
      901 attttccccc acagcgcgta gtcattgggt tcgaagacca cccactgcgt accttcgtcg
      961 atcgattcct ggagaaacag gaagaaccgc cgcacgttga tgtatttcca ttctccatta
     1021 cggtcacctc cgattgtgcg ggcgccccaa actgtcacgg ctccgttgat ggttttgatt
     1081 acgttaaccc cctggggatt gagcacctcc tgattaggtt tgctcacgta atacttgacg
     1141 ttgattgcgc cgcgcaccac gcagtttgcc ggagccttat gcacgccgcg ctcctcgtcg
     1201 gtacgagcat aaacgcctgc catgtgcccg ctgggagcga cgaaggtgcg tccacgatat
     1261 tttgcctcga cgcgaagcgc cggatcggta tcttgtatct gttttgccgg atcaacgacc
     1321 tcaatctgag ggaagtagaa cgcggcgttt ttacttggat gcggtatagg ctcttccccg
     1381 gacccggtgt atgtgattct ctggatatca aagttgttcg ggtcgccaac taccttaggg
     1441 ctgtccagaa tggcgaacac attttcatga ttttcgtctt ccgcataatc cattaacgga
     1501 ttccacacgt cgatacgctt aggcaagccg ggtgcggcaa tcagagcgac ctcgtcaatc
     1561 gattccatgt ttttcaggac gttggggatg tctgcggctg atctcacccg ggcaacgaaa
     1621 caacgtgttc caccattctt gaaaaagcca ttcaccgcgt gggtcagggc atgatgaccg
     1681 gggaacatgg gtctgtatga ttcggttccg gatctgaacg ctgaaaaagg cccaaaacga
     1741 ttggtgtatt cggtgaagtt cgtgcagaat ttggtgtccc aagggtctac ttccacgtta
     1801 aaggctttga ggtaataagg gtgctcccgg ggagcctgtt gcccaggcgc aggttccatc
     1861 cagtcggcat caggagcccc gtcctcgtac gctgtgtcat cctctgtttc agcaggtgcg
     1921 tgtgcgcgag ccacagcagt ctcggcattc tctagctccg tctccttggt agcaactgca
     1981 gtttgcttat ccttgatttg atttctgggg gtgccctcgg tattttgcag agcggtcaat
     2041 tcttctttta gcttgtctct ctctgctctg atccggtccc tcactgcttc cggagtctcg
     2101 ccgccagccc ctgcggctgg aacgcctccc ggagcggcaa gtgccgcacg agcggcgacg
     2161 gggtcatagt agggattcgg gacgggataa taaatagtgt ctgaaacgat gccgataaac
     2221 gccgccgtat ttgttccgac accagcgatg ggttgccggg cggaaggtat ctcttcaacg
     2281 taaacacccg gggcttgata ggtaagcctg gtcatgtgca tcccctttcg tattgaataa
     2341 taaaaagttt cagagaagcg ccacccgcct catcgaaaca ccgctttcaa tgccatttcc
     2401 tctttccgcg ttttccactt tttccggctg ctgaaaccag aagccaggcg ttacttcaaa
     2461 acaagctcga cctgataccc atcatccggc tttctgtctt tcacgcgttt ttccagtacg
     2521 tctcgatgcg aatggacggt ttgctccacc tccagacccg aatgggtatc cctggctata
     2581 agcgtgtatt cgccatcggg gcagtcgagg aagaaaaaac cgccatcaaa acccgactcc
     2641 gttttcttcc atcccctgac acctggattc tccacctcct gctgagcggt cgcccttgtg
     2701 ttgcgggacc ggggagaacg tggcctctcc ggttgcaatt cggattcact gatgtgcgga
     2761 acaaggccca gccgtatgcc gtgcaaaggc tttccgcttg aatccactac tcgtccacag
     2821 atgctcacgt ggtgccttac gatttcccag tccgtccacg ttttcatgat tcggcttcct
     2881 ctggcgtggc gcccatttgc gtcctgaatt tcagtacttt ttcagtgacg agatcgaccg
     2941 gcttctcatc cgcggcctgg acggccaggg ttaccgtgta gttgagcgcc gccttgggtt
     3001 tgccacccag cgcttgccag aattcgccca ggctttgcag ccgccccggc tgaagtgtgg
     3061 tcactggcag tggtagatcc tgcgatacca gctccccctg cagcatttcc tttggaattg
     3121 tccggtaacg tatcagggcg cgaatgactt ccccaagcag ccgatgctcg tcttccgaag
     3181 ggtcggttac gctctggctc ggccaggcgg taatcaggta tgagatatcc atgcgaacag
     3241 gcgcctgttt ttgtaccgtt gttccatcgg atttccgatc gaagtagatt tcgttactgc
     3301 gaaggtccag attttcgcgg atgtcataca ggaacagatc gatcgcgggc agagtgactg
     3361 aagggggagg aaactggtca tcaggggcgc tgaaactgac ttgaacctgg tcggcaagcc
     3421 gtctggggag cttttgcttc agtaatttgg aaagtgtttc gtcgagatcg ttaatcatga
     3481 tcctgccgct aatccatttc gtttcatttc tcgcaacggg ggttggccca cgcctcgctc
     3541 cggctgttcc cgcttatcct tgcaatcctg tcatgtacac gccacacgca acatccatgc
     3601 ccggtttaat cacgtccgat atgattaatt tttaccctta tatgtcaggt tcagcagaac
     3661 aggtgaacct gccgagtaag cgtgaagact cgccaatatg aaaaaaacct gtaaatccca
     3721 atagttacgt cattgggtcg aatttaaccc acctgggtca ggttcagcat taaccaacct
     3781 cagtataaat tcagccgaac tatcagcggg ttagcgacgc gatgcattct ctttgctcgc
     3841 cgccaggtcc tccttatctc ggttgtatga gcctttttcc gtaaatgagg atcaaagcgt
     3901 ctacaaaggg ctgaaagaag atcatgaaaa tgatcggcac aaagcttgct tctcactttt
     3961 atacgcaact tatacttacc cagcttcaaa ggcattcagt tgatcaacac tgtattcatt
     4021 ttcagcgtaa cggctgccgg gcagcggcga aggattcaaa gatgagacag tcattccact
     4081 tgtgctcgcc ggccagcttt gctttccgaa acgcacttgg ggtgcgaagt acaatcgctc
     4141 gagccggggg aacggtttgt acaactgtta ccttgatcac cctctgcggt ttctccttcg
     4201 ccggtcaatt acagctcatg tcggtcaagg agacttcgcc cggtgccgtc accgctaccg
     4261 ttggcgtacg tacaggtggg acgccgaatg cttcggactt ccagttgctg ctcgacggaa
     4321 aaaatgaaat taaggccgga gaagtgaaac cggcctcttc gtctctgctg gaaacctccg
     4381 tgattctctg cgtcgatcag agcgggtcca tggggccggc gcgtatcaag caacttcagg
     4441 acgcgataag gagcgctttg gtcaagccgg aagggggacg cttgagcctg gcattatggg
     4501 catttgattc ggacgtcacg aaacttcacg atttctctga aaatgcggaa gaattatcca
     4561 aggcggccgg taaaatcggc ctcagatctg ccagggacaa tcaaacaaag ctttacgagg
     4621 ctatcgcgct tggtctttcc gagttgcgga attacccgaa aaatggccca aaacgcctga
     4681 ttctgatgac cgatggaaag gatgacggca gcagcattac cgagcaggtc gtggtaaacg
     4741 aagcgaatgc ccagagcgtc atcattgatg cgatagggtt tggcaacgtt tcggaccacg
     4801 acgccaatct gcttgcgcgc ctggcaaaaa gcgcaggtgg atatttcacc cgggtaaaca
     4861 gcgccgacca gctttcccgc gaacttcaaa aattactgag cctgccgcct ccgcgggttt
     4921 tcgatgtcaa ttttcattac aaagcctcag gggaggcaga tcgagcaaag atcgcccaac
     4981 tgaaatttac agcagccgga cagccgccca ttcttctcaa tatccagcaa accctttccg
     5041 cgctccaatc ggtttcgtcc gataggaagg aatctcaacc cgataaggaa aagaattccg
     5101 atctctctat cttgctctgg ataatggtag gactgctggc aatcgtcggc gtttacctac
     5161 tgggtagaag caaaagcaag gaagaggtga agcatgaaat aactcccgaa cctaccccag
     5221 cgccgccccc cgcctcaacc gaacccgaac ccgtaaagcg tcccagaacc atggttgcgt
     5281 tcatgtttcc cccccccgcg aaggggcagc ccgccgcgta tctggacgga atctccggga
     5341 aggcgagagg tgagcaattt cccatcgaac agaaagtgac gcatatcggc gcaggtgatg
     5401 acaacgacct gcggatggac gacgagtatg tatcgcggaa acacgcggcc atccggtacg
     5461 actcgggtgg cctttatctc aacgacagcg gttcaagaaa tggaacgttt cttaacggcg
     5521 ccgagttgca tcactctgca atggcgcttg cccctggcga ccagattcgt gtgggaaaga
     5581 ctacttttcg ggtcagggcg cgtgatagcc aggcgccaag gcgccctgac aaggggccgg
     5641 ggacggttac agaacccacg gtaccgtgac tgctttatgc ggagtattgc gatatccggg
     5701 aaggttaatg gaaggatcga gcaccacagc gttgtccgtt ggagcgcgct ctgaaaccgg
     5761 ttacgtccgg agtgagaatc aggaccggat gagccgtgtg gaagcaccct tcggcgaaat
     5821 ttatatcgta tccgatggaa tgggaggcca tcgggggggt ggtattgcag cggcgcttac
     5881 ggttgaaata ttgggaagaa tgctgtcggc aattccggcg gtatccaacg ctgcctgctc
     5941 agttaaggcg gcattcgaag aggcgaacag ggatgtttac gaaaagggcc atcatggaga
     6001 tgagcaaacg cggggcatgg gcgcgaccgc ggtcgtgctt gcgatcggtg ggccacaggc
     6061 gctggtggcg cacgtgggcg atagccgggc gtaccttttc acacatcgcg aactgcgcag
     6121 gttgaccaag gaccacagcc gtgttcagcg aatggtggat gcgggcatcc tgaccgatgc
     6181 ggaagcgtca tctcatccct cggcgagctt gctggagcgt gccatcggcg ttttgcccga
     6241 cgttgaggtg gatatttccc ctccgatcga cctgaatggg ggcgaattgt ttttgctctg
     6301 ctccgatggg ttgcacgggt atgtttccga ccgcgaaatc ggcgccattc tgagcaggag
     6361 aattccggta caggcaatgg cggacgagtt ggtcgacgcc gcgctggaaa aaggcgggga
     6421 agacaatatc acggttcaat tagtggggta cgaacttgcg gagcggagcg atcagacgcc
     6481 cattgccgga ataccctgat cagcttcaat gcttacgtgt tgcaacatta cgtgttggat
     6541 acgccaagcc gctcgggatt tggctcgtgc cttgccttta gatgtggttg gcaaacgagc
     6601 aagtgccaac ggtattcctt caggaaaatt tgaaacaaag gtatggccat gagcgaaaaa
     6661 ccaaaatcgg gggtattttc acaattattg aagcacatca ttcctggtga aaagtccagt
     6721 acccgtactc ggtattgcga aaagggccat ccgatggacg cgaattggac gacgtgcccg
     6781 tattgcgagg ccgagaagag gaaaaacgat aaaagcatca ttcgtaccac taccaaatcc
     6841 gatcaaattg acaagggtga aaccccgcaa aggagcgcag atatggagcg aggaaccaca
     6901 aagataggta caggttcacc ggaagagcct gatggcagga caaggttcga cacctcaccc
     6961 gagcaagttt ccgaaaagaa agtcgtgggg cgcgagcgaa gaattaccgg agtgctggtt
     7021 actttttctc ataaatacca gggtgagctg tttgtccttt atgaaggacg aaacgtgatg
     7081 ggttcaggtt cttcctgcga tatcagcatt aattgggatc gattggtatc cggcgagcat
     7141 gccatgattc tctgccgtgc cgggagggac gaactgcacg acatgctcag taccaacgga
     7201 acgtttttga acgaggaata cgtgggaaca aagggcgccg acctcgcgga cggcgcgatg
     7261 atcaggatag gcagcacggt gttcgagttc aggaagatta cgtccggcgc gggaaaggga
     7321 ccgggagaaa aggctgaacc cagtgaagaa gacagtgaac tccgcccccc tggcgaaaca
     7381 gacttttaac tatcctctcg tttcaagatt gttagatgaa gtactccgga tcaagcgctc
     7441 gggcagggga gttccgcgaa ggccaagcct gcggaaaata catccttgaa agtgttatcg
     7501 ggaggggtgg catgggaacg gtgtggcgcg caaggaacgt taccagcggg caacctgttg
     7561 ccctcaaggt cgttgcggac gatctgcttg ccgagccgca tattcgggca cgcttcgaga
     7621 acgagatgag gcgccacgcg cgtctggatc atcccaatat tgtccggctg ctcgatacgt
     7681 tttccgtgaa cggtcagcag tgtatggtaa tgaaactaat ggaaggcgaa tcgctggccg
     7741 tgcgcttacg gcgaggtccg gatcatgcgc tccccctgga tgtttcattg cagatatttt
     7801 ccggcgttct tcgcgcgctt gattacgcac atcgtcacgg aatttttcat cgcgacgtga
     7861 aaccttccaa catcctggtg gacgccgcca atgaagcgta tttgcttgat ttcggcattg
     7921 ccctcgctgt tggcgaagcg cggctgacag ggatgggtgt acctgtaggc acccctgcgt
     7981 acatgagtcc ggagcagatt cgcgcacccc ggcagatcga ccataggacc gacgtctaca
     8041 gcgctgcgtg tgttctgtac gaaatgctga caggacgccc gccttttctt tatttgaaag
     8101 tgggtggtac tacagcagaa attgcattgc gctgggctca tgtaaacgaa agacctatac
     8161 ctccgcatga gctgatgcca tcgattcctc ccggaatcag cagaataatc atgatggcgt
     8221 taaataagga tcccaatgag cggccgcaag gttgcgcgga attcctccgg ttgctccttg
     8281 ccgagtttgc cgacaccggg catgttgagg cgcagaagga ggcacagagg cgatgggagg
     8341 agttcgtttc tgattcgacc ctcc
//
LOCUS       DASSHJ010000274         8230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_677178, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000274 DASSHJ010000000
VERSION     DASSHJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677178"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFI43_10470"
     CDS             <1..542
                     /locus_tag="VFI43_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HET7062590.1"
                     /translation="PTSGGIYQPSTQYKPLFEDRRARQVGDTLVIVLNEKTNASKKSN
                     SSTDKNGELNFGIPPIVFGVPITKKVDMGASSANKFAGKGEAASTNNFNGTIAVTVID
                     VLPNNYLVVSGEKQLAMTQGTEYIRFSGVVRPETVINNTVSSIQVADARIEYKANGYI
                     DEAQTMGWLSRFFLTVSPF"
     gene            566..1675
                     /locus_tag="VFI43_10475"
     CDS             566..1675
                     /locus_tag="VFI43_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HET7062591.1"
                     /translation="MTLNRAWRFLFLLWLSCCVGGPAQAERIKDLVSFQGLRPNQLVG
                     YGLVVGLDGTGDQTIQTPFTIQTLTSMLMQMGINIPPGTNMRLRNVAAVMVTATLPPL
                     AQPGQLIDVTVSSMGNARSLRGGTLLMTPLKGADGQVYGMAQGNLLVGGFGAAASGSK
                     VQVNHLSVGRITGGATVERAVPVALAQDDMMRLELNVTDFSTVRRIVDVINGRFGNGT
                     AFALDGRAIQVRTPAGSDPRVAFLAEMESLSISPPQSQARVIVNSRTGSVVMNGAATV
                     EACAIAHGNLSVVISTEPVISQPGPFSSGQTVQAQRSTIEIKQESGMLTMVEGASLAE
                     VVKALNAIGATPQDLLAILQAMKSAGALRAELEII"
     gene            1675..2658
                     /gene="flgJ"
                     /locus_tag="VFI43_10480"
     CDS             1675..2658
                     /gene="flgJ"
                     /locus_tag="VFI43_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HET7062592.1"
                     /translation="MVAPADLSTKFALDVQAVDGLRRVSKKTGPAGLEAAARQFEALF
                     MNMLLKSMREATPQDNLMDSEQSRLYISMLDQQLSQTMAGRSVGLAEIMIRQLRNAVP
                     AEPAQHADNAVGERLRQADGSQQVKSERPVPTEQQSYGAKVSEHQVPQEQPVQQPPQS
                     PPQSGLQSQMQKSEATEFQRKLMIHAREASQATGIPAEFLLGQAALESGWGKRELRSS
                     DGTPSYNLFGIKAGASWTGRVVEAVTTEYVDGVAQKTVEKFRAYHSYAESFRDYVNVL
                     RSSTRYAGVLAQAAQGISPEDFAHGLQRAGYATDPAYGNKLSRIIRNSQGS"
     gene            2831..4750
                     /gene="flgK"
                     /locus_tag="VFI43_10485"
     CDS             2831..4750
                     /gene="flgK"
                     /locus_tag="VFI43_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HET7062593.1"
                     /translation="MGNSILSIGLSALNAAQRGLLVAGHNVSNAATPGYTRQQSIQST
                     SLSQATASGFIGQGVQIDTVKRTYNDLLAHQVAAAKTESSQLDTYLAQMKQIDSLFAD
                     SSGRLGLSPALQDFFNRVQDVATNPAESASRQSMLSNAEVLARRFQSMSSRLDEIENG
                     ANSQIENSVSLINTLSERIAKLNETISLSESAAGGQPANDLRDQRDELVMQLNQEVGA
                     KAIDQGSKGYSVFIGGGQTLVDGSTAFKLKTTASPIDGQRLQLTYVSGANSAPVRESN
                     VDGGKLGGLLQFRNEAIDAARNGLGRVAIGLAGTFNEQHRLGQDIQGNAGGNFFNIPS
                     PMVQGSSKNTGNAVVGADIVSYGSLSISDYRLRFDGANYSLSRLTNGTEAPVQTFIAF
                     PQTIDGISLSLASGAMAAGDEFLVRPTANGAGQIGVAIQDTSLIAAAAPIRTDSSLAN
                     AGTGRISGGAVNAPPPPNPNLKQPVTITFTSSTTFDISGTGTGNPSGVTFTPGSPISY
                     NGWTVQITGSPKAGDIFSVGPNSNGSADNRNTLLLGALQTSNSLAGGTASYQSAYGQT
                     VSLIGNKTRELEVTSKAQAALLSQTQALQQSESGVNLDEEAANLLRYQQAYQAASKVI
                     QTASEMFETLLAATR"
     gene            4870..5805
                     /gene="flgL"
                     /locus_tag="VFI43_10490"
     CDS             4870..5805
                     /gene="flgL"
                     /locus_tag="VFI43_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HET7062594.1"
                     /translation="MRVSSNTGYEMGIAALNRQQAEQVKTLEQISSGRRVLTPSENPA
                     AYVRALEVAQADSANTRHTSNRQYATTSFAILEGSLSEVTNLLQNAQTLAISAGNGAL
                     GDGSRTALAAELRGNLDELMGVANRTDGNGRYLFSGFQGSTKPFVNTGSGIQYLGDEG
                     QRMIQVSASRQLAASESGKEIFERVPASGGGYQSVFKTFSDLVGLLETPVTSPASKTA
                     LYNGLGTAKINLSNALDNVLQIRASVGTRAKELDTLNETGDELSIQYKQQLADLQDVD
                     YAKAISDLTQQQTYLEATQKAFLKVQGLSLFDYLK"
     gene            5873..7261
                     /locus_tag="VFI43_10495"
     CDS             5873..7261
                     /locus_tag="VFI43_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7062595.1"
                     /translation="MIAGNSAPHDYFFYRFPAIAMRAEREFYKDKLRRRMVYGSVQHF
                     PLTPQVKEFFLARQPILDRNEHLIGYKLLFRRAESVQSNAADDWRGALSIIAHMSELG
                     MGNVIGAGRAFFSVDFNVLTHDLVKFLPSQKVVLEIPQMIKITPELVRRVSGMVNAGY
                     TFALDDVITFSDYIKPLLPLVDIVKINTSFMTQADLAIVASKFKQAGKLLLAEKVDSA
                     EQFLNYMELGFDYYQGYYFARPVILSGKKLEPSQLAIIQLMALLAGDADNAKIEHNLK
                     KEVSLCVSLLKLVNSAAMGVTKNIHSLSEALVVLGRRQLQRWLQIQLYVGSSVNPRAS
                     SPLLLLATTRGKLLERVAQKLEPENKTTGDTAFTVGILSLLDSLFGQSMKKILEEISL
                     GEDVTEALLYRKGFYGDLLKLAEYVEKPGETSHLLPPLLARIGLRVEELYPLQLEAFE
                     WSNKLMPYAGEG"
     gene            7371..7685
                     /locus_tag="VFI43_10500"
     CDS             7371..7685
                     /locus_tag="VFI43_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7062596.1"
                     /translation="MATIDLTRAEVESLSGPAVIEFGTSWCGYCRAARPLIASALSNY
                     PAVRHIKIEDGKGRPLGRSFRVTLWPTLIFLKDGKEICRMVRPANSGVVSEVLEKITD
                     AG"
     gene            7892..>8230
                     /locus_tag="VFI43_10505"
     CDS             7892..>8230
                     /locus_tag="VFI43_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7062597.1"
                     /translation="MEGDERESRVIVSHAPGMVFQYVLRESGRYTMPFASNQALNVLG
                     ITAESLQMNPVLFEEIILWKDRKSFRESRAQSAETLSTWNWEGRVWIESYHDVKWVSL
                     RASPRREKGVG"
BASE COUNT         1959 a         2194 c         2364 g         1713 t
ORIGIN      
        1 cgccaacaag tggcggcatc tatcagccga gtactcaata taagccgctg tttgaagatc
       61 gccgcgcgcg ccaagtgggc gataccctgg tgatcgtgct aaacgagaag acaaatgcaa
      121 gcaaaaaatc caatagcagc acggacaaga acggcgagct caattttgga attccaccca
      181 ttgttttcgg cgttccaatt acgaagaaag tcgacatggg ggcatcctcg gcaaacaagt
      241 ttgcggggaa gggcgaggcg gcttccacca ataatttcaa tggcacgatc gccgtgacgg
      301 ttatcgacgt attacctaac aactacctgg tggtgagtgg cgaaaagcaa ctggccatga
      361 cgcaaggcac cgagtacatc cgtttctcgg gggtggtgcg cccggaaacc gtcatcaata
      421 acacggtttc atcgatccag gtagcggatg cgcggattga gtacaaggcc aacggttata
      481 ttgacgaggc gcaaacgatg ggctggttgt cgcgcttttt cctgaccgtt tctccgttct
      541 gaagacaaag ggaagttccg cacatatgac gctgaatcga gcgtggcgat tcctgttcct
      601 gttatggctc agttgttgcg ttggagggcc tgctcaggca gagcgcatca aggacctggt
      661 ttctttccag ggcttgcgtc ccaatcagct cgttggctac ggcttggtgg tcggcctgga
      721 tggcacgggc gatcagacca ttcaaactcc attcaccatc cagacgctca ccagtatgct
      781 gatgcagatg ggcatcaata ttcctcccgg caccaatatg aggttgcgaa acgtggctgc
      841 ggtaatggtc acggcgacgc tgccgcctct cgcccaacct gggcagctta tcgatgtgac
      901 tgtatccagc atgggaaatg cgcggagcct gcgcggaggc acgttgctga tgacgccgct
      961 taaaggcgcc gatggccagg tttacgggat ggcccagggc aatttactgg tcgggggatt
     1021 cggcgctgcg gcaagcggca gcaaagtgca ggtcaatcat ttgagcgtag ggcgcatcac
     1081 tgggggcgca acggtcgagc gagcagtgcc cgttgccctt gcccaggacg atatgatgcg
     1141 cctcgaactt aacgttactg atttctcaac cgtgcggcga atcgtggacg ttatcaacgg
     1201 aagattcggc aatggcaccg ctttcgcgtt ggatggccgg gcgatacagg tgcgcacgcc
     1261 tgccgggagc gatccgcgcg tggcgtttct ggcagaaatg gaaagcctga gtatcagccc
     1321 gccgcagagc caggcaaggg tgatcgtcaa tagccgcacc ggttcggtcg tgatgaacgg
     1381 ggcggcaaca gtggaagcat gtgcgatcgc tcatggcaat ctatccgtgg tgatcagcac
     1441 tgaaccggtc atcagccagc ccgggccgtt ctccagcggc caaacggtac aggcgcagcg
     1501 ttcgaccata gaaatcaagc aggaatcagg catgctcacg atggtcgagg gcgcttccct
     1561 ggccgaagtg gtcaaggcct tgaacgccat tggagccacg ccccaggatc tgctcgcgat
     1621 attgcaagcg atgaaatcag ccggagcgct ccgggcagag cttgaaatta tttgatggtc
     1681 gctcctgccg atctctccac aaaattcgcc ctcgacgtgc aagctgtgga cgggcttcga
     1741 agggtctcga aaaaaacggg acccgcggga ctcgaagctg cagccaggca atttgaagcg
     1801 cttttcatga atatgctact gaagagcatg cgtgaagcca cgccccagga caacctcatg
     1861 gatagcgaac aaagccgcct gtatatttcg atgcttgatc agcagttatc gcaaaccatg
     1921 gctgggcgca gtgtgggact tgccgaaatc atgatacgcc agcttcgcaa tgccgtgcca
     1981 gccgagcccg cgcaacatgc ggacaacgcc gttggggaac gattgcgtca ggcggacgga
     2041 agccagcagg tgaaatcgga acggcccgtg cctacagagc aacagtcgta cggggcgaaa
     2101 gtttccgaac accaggttcc acaagagcag ccagtccagc agcctccaca atcgccgcct
     2161 caatcgggac tgcaatcgca gatgcaaaag tcggaggcaa cggagtttca gaggaagctt
     2221 atgatacacg cccgggaagc cagtcaggcg accgggattc ctgcagagtt cctgctgggg
     2281 caggcggcgc tggaaagcgg ctggggcaaa cgagagcttc gctcgtcgga cggaacgccc
     2341 agttataatc tgttcggcat aaaggctggc gccagctgga cagggcgcgt ggttgaagcg
     2401 gtcacgacgg aatatgtgga tggggtggcg cagaagaccg tcgaaaaatt ccgcgcttat
     2461 cattcctatg cggaatcctt ccgcgactat gtcaacgttc tccgcagcag tacccgttat
     2521 gcaggcgtgc tggcgcaggc ggcccaggga atcagcccgg aggactttgc gcacggcctg
     2581 caacgcgcgg gctatgcgac tgatcccgcc tatgggaaca aattaagcag gattatcagg
     2641 aattcccagg gctcatagag caagagaggt acgaagacga taccttcggg ccatcgcttc
     2701 ctgctatcgc caatcttgcc gatagcgtca catgcaacct catacgtaat tcatacgtaa
     2761 gcgcggctaa atatttttaa tccggtgccg tttacattga taccggccag tggcgtagat
     2821 cgagtaaaac atgggtaaca gcatcctcag catcggcctc agtgcgctca acgctgccca
     2881 gcgcggattg ctcgttgccg ggcacaacgt cagtaatgcc gctactccgg ggtacacgcg
     2941 ccagcagagt atccagtcga ccagcctgtc ccaggcaact gcaagcggat tcattggcca
     3001 aggcgtgcag atcgatacgg taaagcgaac ttataacgat ctcctggccc accaggtcgc
     3061 cgcagcaaag accgagtcca gccagcttga cacttacctg gcccagatga agcagatcga
     3121 cagccttttc gcggactcca gcgggcggct tggcctgtcg ccggcgctcc aggatttttt
     3181 taacagggta caggacgttg ccaccaaccc cgcggaaagt gcctcccggc aatcaatgct
     3241 ctcaaacgcc gaggtgctgg cacggcggtt tcagtcaatg agcagccggc ttgacgaaat
     3301 cgaaaatgga gcgaacagcc agatcgagaa cagcgtttca ctcatcaaca ccttatcgga
     3361 aaggattgcc aaactgaacg aaacgatcag cctttctgaa agcgctgcgg gcggccaacc
     3421 ggcaaatgac ttgcgcgatc agcgcgatga attggtcatg cagcttaacc aggaggtggg
     3481 tgcgaaagcc attgatcagg gcagcaaggg gtacagcgtt tttatcgggg gagggcaaac
     3541 cctggtggat ggttcaacag cttttaaact caagacaact gcttcgccga ttgacggcca
     3601 acgcctgcag cttacatacg tttctggcgc caatagcgca cccgttcggg aatcaaacgt
     3661 ggatggcggc aaactggggg ggctgctgca atttcgcaac gaagcgatcg atgccgcccg
     3721 caacgggctg gggcgcgttg ccatcggcct cgcgggcact ttcaatgaac agcaccggct
     3781 gggacaggat attcagggca atgcaggcgg gaacttcttt aacatcccaa gcccaatggt
     3841 tcagggatca tcgaagaaca caggcaacgc cgttgttggg gccgacatcg tgagctatgg
     3901 ctcgctttcc atcagtgatt accggttgcg tttcgatggc gccaattaca gcctcagccg
     3961 attgacaaat ggtacggaag ctccggttca gacctttatt gcctttcctc agaccatcga
     4021 tggtatttcc ttgagtctcg cttcaggtgc catggctgct ggagatgaat ttctcgtccg
     4081 cccgactgca aacggcgccg gccagattgg ggtcgccatc caggatacga gcttgatcgc
     4141 cgcggctgca cccatccgaa cggactcatc gctcgctaat gccggcaccg gacgcatcag
     4201 cggtggagcg gtaaatgcgc cgcctccgcc caatccaaat ctcaagcagc cagttacaat
     4261 cacgtttacc agttccacca cgtttgacat aagcgggact gggaccggta atccttcggg
     4321 tgttaccttc actcccggga gccccatcag ctataacgga tggactgtgc aaatcactgg
     4381 ttccccgaag gcgggagata tctttagtgt tggccccaat agcaatggat cggcggataa
     4441 ccgcaacaca ttattgctcg gtgccttgca gaccagcaac agcctcgcgg gcggaacggc
     4501 aagctatcag agcgcttatg gccagacggt cagcctcatt ggaaacaaga cccgcgagct
     4561 cgaagtgacc agcaaggctc aggcagccct gctgagccag acgcaggctt tacagcaatc
     4621 ggaatcgggc gtcaatctcg acgaagaggc ggcgaacctg ctgcgctacc agcaggcata
     4681 ccaggcggca agcaaggtga tacaaacggc cagtgagatg ttcgagacac tgctcgcagc
     4741 aacacgataa ataatgaggg atatcgttcg cctttatcac gctgctgttt acggacgatc
     4801 cccatgcagg cattgagcga gagtgcggac gatgcgtgga aaaaggaata gaaaagggag
     4861 gagcaggaca tgcgggtgag cagtaatacc ggctatgaaa tgggcatcgc tgcgctcaat
     4921 cgccaacagg ctgagcaggt caaaaccctg gagcagatca gcagcggacg ccgtgttctc
     4981 actccatcgg agaatccggc tgcttatgtc cgggcgctgg aagtggcgca ggccgattcc
     5041 gccaataccc gccatacttc gaaccggcag tatgccacca ccagtttcgc aatactggaa
     5101 gggtcgctca gtgaggttac aaaccttctg cagaacgcgc agacgctcgc catatccgcc
     5161 gggaatggcg cgcttggtga cgggagtcgc actgcccttg cagcagagct gcggggcaat
     5221 ctcgacgaac tgatgggggt tgccaatcgt accgacggta acggccgcta tctgttttcc
     5281 ggattccagg gctcaaccaa gccgttcgtg aatacgggca gcggcatcca gtatttgggc
     5341 gacgaaggcc agcgcatgat acaggtgagt gcatcacgcc agttggcagc gagcgaatca
     5401 ggaaaagaga tattcgaacg cgttccggct tccggaggag gataccagag cgtgttcaag
     5461 acattttcgg acctggtcgg cttgcttgaa acgccagtaa ccagtcctgc tagcaagacc
     5521 gcgctttaca atggtctcgg caccgcgaaa atcaaccttt ccaacgccct cgataatgta
     5581 ttgcaaattc gcgcgtcggt tggcacgcgt gcgaaggaac tggatacgct caacgaaacc
     5641 ggtgacgagt tgagcatcca gtacaagcag caacttgcag atctccagga tgtggattat
     5701 gccaaagcca ttagtgacct gacccagcag cagacctacc tggaagcgac gcagaaggca
     5761 tttctgaaag tgcaggggtt atcattattt gattatttaa agtgactttc acggtcgccg
     5821 tgatagcgcg agggctcacg cctggggtgg ttgaagtgag ggtaagcgga ccgtgattgc
     5881 gggcaattcg gcccctcacg actatttctt ttaccgtttc ccggcgatag cgatgcgagc
     5941 agagcgggag ttttacaaag acaaattgag gcgtcgtatg gtctatggaa gcgttcaaca
     6001 tttcccgctt actcctcaag tgaaggagtt tttccttgca cgccaaccga tcctggaccg
     6061 gaacgaacat ctcatcggtt acaagctact gttccgccgc gccgaatccg tgcaatcgaa
     6121 tgctgccgat gattggcggg gcgctctttc catcattgcc cacatgtcgg aacttggcat
     6181 ggggaacgtg atcggcgccg gccgcgcatt ttttagcgtg gatttcaatg tgctgacgca
     6241 tgacctggtt aaatttctgc caagtcaaaa agtagtactt gaaattcccc aaatgatcaa
     6301 aataactcca gagcttgtcc ggcgcgtatc aggtatggtt aacgccgggt ataccttcgc
     6361 cctggacgac gtaataacct tctcggacta catcaagccg ctgctcccgc tggtcgacat
     6421 cgtcaagatc aatacttcgt tcatgaccca ggcggatctc gccatcgtgg ccagcaaatt
     6481 caagcaggct gggaaattgc tattggcgga gaaagtggac tccgctgaac aattcctgaa
     6541 ctacatggaa ctcgggtttg actattacca gggttattat ttcgccaggc ccgtcattct
     6601 gtccggaaaa aaactggaac cctcccagct cgctatcatc cagttgatgg cactcctcgc
     6661 cggggacgcg gataacgcaa aaatcgagca caacctcaaa aaggaggttt cgctttgtgt
     6721 atccttgctg aagctcgtca attctgcagc gatgggtgtc acaaagaata tccactcatt
     6781 gagcgaagcg ctcgtagtgc tcgggcgccg ccaattgcag agatggcttc aaattcagct
     6841 ttacgtaggt tcgtccgtaa accctcgcgc gtcatcgccg cttttattgc tggcgacgac
     6901 acgtggaaaa cttcttgaac gggttgcgca aaaacttgaa cctgaaaata aaacgactgg
     6961 ggatactgct tttacggttg gcattctatc gctgctggat tccctgtttg ggcaatccat
     7021 gaaaaaaatc ctggaagaga tcagtttggg ggaggacgtc accgaggcat tgctgtatcg
     7081 caaggggttt tacggagatc tgctcaagct cgcggaatat gtcgaaaaac cgggagaaac
     7141 gagccacttg ctgccgcccc tgcttgcccg catcggattg cgagttgaag aactttatcc
     7201 gctccagctt gaagcgtttg aatggagcaa taagctgatg ccttatgcgg gcgaaggatg
     7261 atctgctagg ctcgctataa aggaattatc acgattgcgc tgctattcat cactgttgtt
     7321 gaattcctta gagagctgga aattcaggct aacctgagtt attgtcaaga atggcaacca
     7381 ttgacctcac acgcgcggag gtggagtctc tttccggtcc cgcggttata gagttcggca
     7441 catcctggtg cggttattgc cgggctgccc gacccttgat cgcctccgcg ctttcaaact
     7501 atccagccgt gcggcacatc aagatcgagg acgggaaggg gcgtcctctc ggacggtctt
     7561 ttcgcgtaac tctttggcct acattgattt ttttgaagga tggaaaggag atctgccgaa
     7621 tggtccgccc cgcgaactca ggggtcgtga gtgaagtcct cgaaaaaatc accgacgccg
     7681 ggtaacttca acaagatcat tatccggata aggggagcca atctttcgag aggttcccat
     7741 tttcactgct gccaggcagt tgaatgctgg atgccgggac tgtaccattc cttcaatcga
     7801 aattgcactg ggtttgagtc gtagatcgcc ccacacccgc ggagtgcagg tgatgggacg
     7861 ctaaacgaaa gtctagaaag gatcaggata aatggagggt gacgaaaggg aatcccgcgt
     7921 cattgtttcg cacgcgcccg ggatggtttt tcagtacgta ttacgcgaaa gcgggcggta
     7981 tactatgccg ttcgcaagca atcaggcgtt gaatgtgctc ggcatcacag ctgagagtct
     8041 tcagatgaat cctgtgttgt tcgaagagat catcctctgg aaggaccgta aaagttttcg
     8101 cgagtcgaga gcgcaatcgg cggagacctt aagcacctgg aactgggaag gccgagtgtg
     8161 gatcgagtct tatcatgacg tgaaatgggt cagcctgcga gccagtcctc gacgcgagaa
     8221 gggggtgggt
//
LOCUS       DASSHJ010000275         8875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_603955, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000275 DASSHJ010000000
VERSION     DASSHJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603955"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFI43_10510"
     CDS             complement(<1..806)
                     /locus_tag="VFI43_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7062598.1"
                     /translation="MIGMRAAFALVVSVAMAGCGKNSQAVSQTQHPPALPVSVIEVQP
                     ATVPISAEAVAQTEGAKEVEIRPRVGGILLKRLYEEGAPVKAGQPMFQIDPVPYQYAL
                     AQAKAQLAEQKARMAQAAREENRLRELLAVQAVSQREYDNAVSDDAIAHAAVQAAEAR
                     ARDAELNLSYTSVTAPVNGISGRFRFSEGALVEANSSLLTTIVQLTPIWVRFSLSDNE
                     LAQIGGPLTEKKVKKVTLILPDGTEYGKEGELNFAASQIDPALGTQQLRAT"
     gene            958..1590
                     /locus_tag="VFI43_10515"
     CDS             958..1590
                     /locus_tag="VFI43_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7062599.1"
                     /translation="MARKTKEDAELTRQRLIEAARQVFLEHGVSRTTMEHIAARAGVT
                     RGAIYWHFSNKLDLFQAMREQVFLPLIDRMDDTLLVEGSEDPLTCIENFLCGTIHILT
                     DSAETRQIYEIMMMKCEYVDEFAAVLQQILMNCSAILEKLQQVYERAQEQDQLHVSHQ
                     PAQLAIDTHLFFIGLLHMWVKDTSGHQFRWRAIELVRAHMCLRRKQHSTA"
     gene            1824..2327
                     /locus_tag="VFI43_10520"
     CDS             1824..2327
                     /locus_tag="VFI43_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7062600.1"
                     /translation="MRLTTKGRFAVTALLDLAMQRTTRPVTLAEISQRQQISLSYLEQ
                     LFAKLRQRALVESVRGPGGGYCLAKDMGQVSVAEIILAVDEPIDSTQCNGKENCHNDK
                     KCMTHDLWVKLNALIFDHLGAITLKQLVEEQKARQNGVVQMIDMRKTLPERDRSVPER
                     ERAAATA"
     gene            2398..2739
                     /locus_tag="VFI43_10525"
     CDS             2398..2739
                     /locus_tag="VFI43_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET7062601.1"
                     /translation="MTITLTENAARQIRKQLGKRGKGLGLRLGVKKQGCSGFAYTFDY
                     ADELRAGDQVFESNGANVVIDVSSLVYLNGSNVDFVRDGLNDSFRVGNPNVDNTCGCG
                     ESFSVKEAAKA"
     gene            2854..4290
                     /gene="sufB"
                     /locus_tag="VFI43_10530"
     CDS             2854..4290
                     /gene="sufB"
                     /locus_tag="VFI43_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7062602.1"
                     /translation="MSAVLQNLVNQPYKHGFVTDIESDVAPKGLNEDIIRLISGKKSE
                     PEWLLEFRLNAYRHWLKMEEPKWPNVKYPKIDFQAISYYAAPKPKKKLNSLDEVDPEL
                     LRTFEKLGVPMHERAALAGVAVDVIFDSVSVATTYKQKLAEVGIIFCSISEAVHNHPE
                     LVRKYLGTVVPVGDNYYAALNSAVFTDGTFCFLPKGVKCPMDLSTYFRINTEESGQFE
                     RTLIVAEEGSSVSYLEGCTAPKFDTNQLHAAVVELVALDNADIKYSTVQNWYAGDENG
                     LGGIYNFVTKRGLAKGVNSRISWTQVETGSAITWKYPSCILQGENSVGEFYSVAVTNN
                     YQQADTGTKMIHIGKNTRSTIVSKGISAGHSNNSYRGLVRIAPTASGARNYSQCDSML
                     IGDQCGANTFPYIQVRNQEAKVEHEASTSKIGEDQLFYFAQRGIGSEEAVSMIINGFC
                     KDVFQQLPMEFAVEATKLLGFKLEGSVG"
     gene            4287..5081
                     /gene="sufC"
                     /locus_tag="VFI43_10535"
     CDS             4287..5081
                     /gene="sufC"
                     /locus_tag="VFI43_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7062603.1"
                     /translation="MIYPDSKVLLEVRDLHATVNGVEILKGIDLTVRTGEIHAIMGTN
                     GSGKSTFAKVLAGHSAYQVTGGSILFEGRNLFDLKPEERARAGVFLAFQYPVEIPGVA
                     NSHFLRLAYNTVQAERGREELDPLEFEDFVREKMKLLEMKPDFLDRGVNEGFSGGEKK
                     RNEILQMALLEPKLAILDETDSGLDIDALRVVTNGVNKLANKDNAIILVTHYQRMLNY
                     ITPDFVHVMQSGRIIKTGGKDLALDLEAHGYEWVGTGKNAPARVGA"
     gene            5084..6397
                     /gene="sufD"
                     /locus_tag="VFI43_10540"
     CDS             5084..6397
                     /gene="sufD"
                     /locus_tag="VFI43_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7062604.1"
                     /translation="MDMVVGKNYLESLLQGEPQLRPSPLSWFNNIRASAVERAGTLKI
                     PTTRDEEWRFTDISPLAKLSFQPVQEAPEVKKEDIARFYLDEAQATLVFVDGHYMPRL
                     SRGSNTASTELVVTNLREALMTHPREIESHLGRHAAFEGKLFSALNTAFLHDGALVIV
                     PRNEAIERPVHLLFVATQAESASHPRCLLIAQSGSAVTVVEDYVTLQEGAYITNAVTE
                     IAIEDNAQVDHTRVQRDSMNAFHIANCAARLGRSSRYRSVSAAFGARISRYDLNAVMA
                     AEGAECDVDGLALIKERQLADTHTSIDHAKPDGTSRQLHKCIVDGAAHAVFNGKIIVR
                     AGAQHTDSSQSSRNLLLSARAHVDTKPQLEIFADDVKCAHGATVGQLDTEELFYLKSR
                     GLSALGARNLLTYAFGAEVIDRIPVASLKRELERTVLEQTQRVEQ"
     gene            6394..7653
                     /locus_tag="VFI43_10545"
     CDS             6394..7653
                     /locus_tag="VFI43_10545"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7062605.1"
                     /translation="MNMVEPPLKSEFGPVLDVERIRADFPILKLEVEGKPLVYFDNAA
                     SSQMPQQVIDRLVHYQTAQHANINRGVHYLSETATAEYEGSRRKLQHFLNAREEREIV
                     FTSGTTDSINLVMQGYGRKFIGPGDEIVLTALEHHSNIVPWQMLAEEKGAKLRVVPIN
                     DAGELLIDEYEKLFNECTRFVSVLHVSNALGTVNPVKQMIAFAHSKGVPVFVDGAQAA
                     PHMSVDVQDLDCDFYAFSGHKMCGPTGIGVLYGKADLLERMQPFRGGGDMILSVTFEK
                     TIYNSIPHKFEAGTPPIAAAIGLGVAVDYLSAISLDAIARHELKLLDYATEKISELEG
                     VRIIGTAAAKTAVLSFEVKGVHPHDVGTLLNQEGVAVRTGHHCTQPLMLRLKVPATTR
                     ASFAFYNTMAEVDAFIDALRAVQKIFA"
     gene            7653..8135
                     /locus_tag="VFI43_10550"
     CDS             7653..8135
                     /locus_tag="VFI43_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET7062606.1"
                     /translation="MNSKSLYQEVILDHNKKPRNYGTLDHASHHAVGHNPLCGDHLDI
                     ALHLEGERIDQIAFHGESCAICKASASMMTTAVKGKTSADAETLIQEFRDMAVGKLNL
                     EAPNHLGRLTVFAGVRDLPTRVKCAILPWHTLHAALHSVTTTSTEAEDDPMHAAIGDA
                     "
     gene            8153..8713
                     /locus_tag="VFI43_10555"
     CDS             8153..8713
                     /locus_tag="VFI43_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET7062607.1"
                     /translation="MPKIAEIEGTPNRNALKFILREPLTWGVTRSYDNAEQAKDDPLA
                     AALFEIDHVTNVFYVDRWITITQDGGADWQDLAREVADPIRAAPAADRQSAATVAAAN
                     TAMAGLSPEDQERLETINALLDEEVRPYLQNDGGDLHVLSLEGNKLSVHYQGACGTCP
                     SSITGTLRGIQNMLRSIEPDIEIVAL"
BASE COUNT         2087 a         2315 c         2524 g         1949 t
ORIGIN      
        1 gttgctcgca attgctgcgt tcccagcgca ggatcgatct ggctcgcggc gaagttcaat
       61 tcgccttcct tcccgtattc cgtgccgtca ggcagtataa gcgttacttt ttttactttt
      121 ttctcggtca ggggtccgcc aatctgtgca agttcgttat cggacaggct gaagcgcacc
      181 catatggggg ttagctggac tatcgtggtc aggaggctgc tgtttgcttc gaccaatgcg
      241 ccttcggaaa accggaagcg cccggagatt ccatttaccg gcgcggttac cgatgtatag
      301 gaaagattga gttccgcatc gcgcgcgcgg gcctcggctg cctgcacggc ggcatgcgca
      361 atggcatcat cggataccgc gttgtcatat tctcgctggc tgaccgcctg gacggcgagc
      421 agttcgcgca accggttttc ctcgcgcgcg gcctgcgcca tgcgtgcctt ttgctcggca
      481 agctgtgctt tcgcctgagc caatgcatat tgatagggca ccggatcgat ctggaacatt
      541 ggctggcccg cttttacagg tgccccttcc tcatacaggc gcttgagaag gatgccgccg
      601 acccggggtc ttatctcgac ctccttcgcg ccttcggtct gggcaaccgc ttctgcactg
      661 atgggtactg tggcaggctg gacctcgatc acgctgacgg gaagagcagg gggatgttga
      721 gtttgactga ctgcctggct gttttttccg cagcctgcca tggcaacact caccaccagc
      781 gcaaaggcag ccctcatccc gatcatcgca atagagctta acggtttatt gttatatctc
      841 ccgagcgaaa gcatcaatca aactccatca gaacaaacag ttgttgcata cattcgtgta
      901 tgtatgtaaa ttatcaacta aagaatcccc cttgtaaata ctgttatcac caggaaaatg
      961 gcacggaaga cgaaagaaga tgcggaattg acgcgccaga gattgatcga ggcggcccgg
     1021 caggtttttc ttgagcacgg cgtcagccgt acgacaatgg agcatattgc tgcgcgggca
     1081 ggcgttaccc gaggagcgat atactggcat ttcagcaaca agctcgatct gtttcaggca
     1141 atgcgcgaac aggtgttcct gccattgata gatcgcatgg atgatacgct gctggtcgaa
     1201 ggctcggaag atcctcttac gtgcatcgaa aattttcttt gcggcacgat ccatatattg
     1261 acggatagtg ccgaaacgcg ccagatttac gagatcatga tgatgaaatg cgaatatgtg
     1321 gatgaattcg cagccgtgct acagcagatc ctcatgaatt gctccgccat tctcgagaag
     1381 ctgcaacagg tttacgagcg cgcccaggag caagaccagc ttcatgtttc ccaccaaccg
     1441 gcgcaactgg ccattgatac gcatttgttc ttcatcggcc tgcttcacat gtgggtcaag
     1501 gataccagcg gccatcaatt ccgctggcgt gcaatcgagc tggtaagagc gcatatgtgc
     1561 ttgcggcgaa agcagcattc gaccgcctaa aaatctgctt gcatccagtc ttcgatcgct
     1621 cctccattgc cttgcggtaa ttgtcaggcg cgtcccttct gacttttccg ccctccgctc
     1681 cttactttcc cgacatgttg cttgtatagt tgactaaatc gatcaagtat cttatagttg
     1741 actaaatcag tcatctattg gctgaactgg ctattcatcg atatgtctat taactgaatg
     1801 acgagagcgg aaaggagttc atcatgaggc ttacaacgaa aggacggttt gcggtaacag
     1861 cgctattgga tcttgccatg cagcggacca ctcgcccggt cacgctagcg gaaatcagtc
     1921 agcgtcagca aatctcgcta tcttatcttg agcaactgtt tgccaagctg cgccagcgcg
     1981 ccctggtcga aagcgtgcgg ggacccggtg gcggatattg tcttgccaag gatatggggc
     2041 aggtttcggt tgccgagatc attcttgctg tcgacgagcc catcgattcg acgcagtgca
     2101 atggaaagga aaactgccac aacgacaaga agtgcatgac gcacgacttg tgggtaaaac
     2161 tcaatgctct catttttgac catcttggcg cgattacgct caagcaattg gtggaggagc
     2221 agaaagcgag acagaatggc gtcgttcaaa tgatcgacat gcgcaagacg ctgccggagc
     2281 gggaccgcag cgtgccggaa cgcgagcggg cggcagcaac tgcatgattc aaatccggct
     2341 tcgccttcat ttttgacgcg cgcgctgtga caggagaatg gagagctgga gaaggcaatg
     2401 acgatcacac taaccgaaaa tgcggcaaga caaatccgga agcagcttgg gaagcgcggt
     2461 aaagggttgg ggctgcgtct cggcgtgaaa aaacaaggct gctccgggtt tgcatataca
     2521 ttcgattatg cggacgagct tcgggcgggc gatcaggtgt tcgagtccaa tggtgcgaac
     2581 gtggtgattg acgtcagcag cctggtttac ctgaacgggt cgaacgttga ttttgtcagg
     2641 gacgggctca atgactcgtt cagggttggc aacccgaacg tcgacaacac gtgtggctgc
     2701 ggagaaagct ttagcgtgaa agaggcagcc aaggcttagc cgcatccagg gtagacggaa
     2761 taattacagc gacagcgcgt gtccgaattt cttttcagtt caggtttata tgggtttata
     2821 tagacgtatt caggcgacta ttggagcaat ctgatgagtg cggtattaca aaatcttgtc
     2881 aatcagccct acaagcatgg gttcgtgacc gatatcgagt cggatgttgc tccaaaaggg
     2941 ctgaacgaag acatcatccg gctgatctcg gggaaaaaga gcgagccgga atggttgctc
     3001 gagtttcggc tgaatgcgta ccggcactgg ctgaaaatgg aagagccgaa gtggccgaat
     3061 gtgaagtatc cgaagataga cttccaggcg atcagctatt acgctgcgcc caagcccaag
     3121 aagaagctga acagcctgga tgaggttgat cccgaactgt tgcgcacgtt cgaaaagctg
     3181 ggcgtgccca tgcacgagcg cgcggcactg gccggggtag cggtggacgt gatttttgac
     3241 agcgtatcgg tcgcgaccac atacaaacaa aagctggccg aggtcggcat cattttctgc
     3301 tccatctccg aagccgtcca taatcatccg gaactggtca ggaagtacct tggcactgtg
     3361 gtgccggtgg gagataacta ttacgctgcg ctcaattccg ctgtgtttac ggacggcacc
     3421 ttctgctttc tcccgaaagg ggtgaaatgt cccatggatc tttccacgta cttccggatc
     3481 aacactgagg agtcagggca gttcgagcgt acgctgatcg ttgccgagga ggggtcgtcg
     3541 gtttcttatc tggagggctg cacggcccca aaattcgaca cgaaccagct tcatgcagca
     3601 gtggtcgaac tggttgccct ggataatgcc gacatcaagt attcgacagt gcagaactgg
     3661 tatgcgggcg atgagaacgg cctcgggggc atctacaact ttgtgaccaa gcggggtctg
     3721 gcgaagggtg tgaattcccg tatttcctgg acccaggttg aaaccggttc ggcaattacc
     3781 tggaaatatc catcctgcat cctgcaggga gaaaactcgg tcggcgagtt ctattctgtg
     3841 gccgtcacca ataattatca gcaggcggat actggcacca aaatgatcca catcgggaaa
     3901 aacacccgga gcaccatcgt cagcaagggt atctccgcgg ggcattcgaa caacagttat
     3961 cgcgggttgg tcagaattgc gccgaccgct agcggcgcgc gcaactactc ccaatgcgac
     4021 tcgatgctga tcggagatca atgcggcgcg aacacgttcc cttacatcca ggttcgcaac
     4081 caggaagcca aggtcgagca tgaagcgtcg acgtcgaaga tcggggaaga tcaactgttt
     4141 tatttcgctc agcgcggcat tgggagcgag gaagcggtgt cgatgatcat caatggattc
     4201 tgcaaggacg tttttcagca attgcccatg gagttcgcgg tcgaggcaac caagctgctc
     4261 ggcttcaagc ttgaagggag cgtgggatga tttaccccga tagcaaagtc ctgcttgaag
     4321 tgcgcgatct acatgcgacg gtgaacgggg tcgagattct caagggaata gatcttacgg
     4381 tgagaaccgg cgagatccac gcaatcatgg gcacgaacgg ctccggcaag agtacgtttg
     4441 caaaagtgct cgcaggacac agcgcgtatc aggtgacggg aggctcgatc ctgttcgaag
     4501 gcaggaacct cttcgatctt aaacctgagg agcgcgcgag ggccggtgtc ttcctggcgt
     4561 tccagtatcc agttgaaata ccgggtgtgg cgaatagtca ctttctgcgg ctcgcctata
     4621 acacagtgca agccgaacga ggcagggaag agcttgatcc gctcgagttt gaggattttg
     4681 ttcgggaaaa gatgaaattg ctcgaaatga agcccgattt cctggatcgc ggggtcaacg
     4741 aaggattttc cggaggcgag aagaaacgca acgagatact ccagatggcg ttgctggagc
     4801 caaaacttgc cattctcgac gaaacggatt cgggtctcga catcgatgcg ttgagagtcg
     4861 tcacgaacgg ggttaacaaa cttgccaaca aggacaacgc aattatcctg gtcactcatt
     4921 accagcgcat gctcaactac atcacccctg attttgtgca cgtgatgcag tccggccgca
     4981 tcatcaagac aggcggaaaa gacctcgcgc ttgatctgga ggcgcacggc tatgagtggg
     5041 taggaaccgg aaaaaatgca ccagccaggg ttggcgcata aatatggaca tggtggtggg
     5101 caagaactat ctcgaaagct tgcttcaggg cgaaccccaa ttgcggccca gtccgctgtc
     5161 atggttcaac aatatccgcg cgagtgcagt ggaacgcgcc ggaacactca agatacccac
     5221 aacgcgcgac gaagagtggc gctttacgga catctcgccc cttgccaaac tttcgttcca
     5281 gcccgtgcaa gaggctcccg aggtaaaaaa ggaagatatt gcgcgctttt acctggatga
     5341 agcgcaggcc acgctcgtgt ttgtcgacgg tcactacatg ccgcggctat cgaggggatc
     5401 gaacacggcg agcacggagc ttgtcgtgac caatctacgt gaagcgctga tgactcatcc
     5461 gagagaaatt gagtctcatc tgggccggca cgctgctttc gaaggcaaac tcttctcggc
     5521 gctcaatacc gcgttcctgc atgatggcgc gctggttatc gtgcctcgaa acgaggcaat
     5581 cgagcgccca gtacacttgc tcttcgttgc aacacaagct gagtcagcaa gccacccccg
     5641 ttgcctgttg attgcgcagt cggggagcgc agtgacggtt gtcgaggatt atgtcacatt
     5701 gcaggagggt gcttatatca ccaatgcagt aaccgaaatc gcgatcgagg ataacgcgca
     5761 ggtcgaccat acgcgggtcc agcgcgacag catgaatgcg tttcatattg caaactgcgc
     5821 tgcacggctg gggcgctcga gccgttatcg gtccgtcagt gccgcgtttg gcgcacgtat
     5881 ttcgcgctat gacctgaatg cggttatggc tgccgaggga gccgaatgtg acgtcgacgg
     5941 gcttgccttg atcaaggaac ggcaactggc cgacacccac acttcgatag atcatgcaaa
     6001 gcctgacggt acgagccgtc agttgcacaa gtgcatcgtc gacggcgccg cgcatgcggt
     6061 ttttaacgga aagatcattg tgcgggcagg ggcccagcat accgattcat cccaatccag
     6121 tcgcaacctg cttctgagtg caagagcgca tgtcgacacg aagccgcaac ttgaaatttt
     6181 cgcggatgat gtcaaatgtg cccatggcgc aacggttggg caacttgaca ccgaggagtt
     6241 gttttatctc aagagccgtg gactatccgc cctgggagcc cgcaatctgc tgacctacgc
     6301 gttcggagca gaggtcatcg atcgcatccc tgtcgcgtcg ctcaagcgtg agctcgaaag
     6361 aacggtgctg gaacaaacgc agagagttga acaatgaata tggtagagcc gcccttgaaa
     6421 tctgaattcg gcccggtatt ggatgtcgaa cgcatccgtg ccgacttccc aatattgaag
     6481 ctcgaggttg agggtaagcc gctggtttat ttcgacaacg cggcgtccag ccagatgccg
     6541 cagcaggtga tcgatcgcct cgtgcattat cagaccgctc aacatgccaa tatcaaccgt
     6601 ggggtgcact acctgtcgga gacggcgacc gccgagtacg aggggtcgcg ccgcaaactg
     6661 cagcatttcc tgaacgcgcg cgaagaaagg gaaatcgtct tcacaagcgg tactacggat
     6721 tcgatcaacc tggtgatgca gggctacgga cgtaaattta ttgggccggg cgacgaaatc
     6781 gttctgactg cgctcgagca ccattcaaat atcgtgccct ggcagatgct tgcagaagag
     6841 aagggcgcga agctccgcgt cgtgccaatc aacgatgcgg gcgaactcct gatcgacgaa
     6901 tacgagaagc tttttaacga atgcacccgg tttgtcagcg tgttgcatgt gtccaacgca
     6961 cttggcacgg ttaatcccgt caagcagatg attgcctttg cccattcgaa aggagtgccg
     7021 gtattcgtcg acggggccca ggcagcgcct cacatgagcg tggacgtcca ggatcttgat
     7081 tgcgactttt atgctttttc gggccacaag atgtgcggtc cgacgggcat tggcgttctc
     7141 tatggcaagg cagacctgct tgaacggatg cagccgttca ggggcggggg agacatgatc
     7201 ctctcggtaa cattcgaaaa aaccatttac aattcgattc cgcataaatt tgaagcaggc
     7261 acgcctccca ttgcagccgc aatcggattg ggggtagccg ttgactatct ctctgcgatc
     7321 agtctcgacg ctattgccag gcatgagctc aaacttctcg attacgcaac tgaaaagata
     7381 agtgagctgg agggcgtgcg tatcattgga accgcggctg cgaaaactgc agtactgtcc
     7441 ttcgaggtga agggggtgca tccacatgac gtgggtacgc ttctgaatca ggaaggcgtt
     7501 gccgtgcgga ccggtcatca ttgcacgcag cccttgatgc ttcgattgaa ggttccagct
     7561 actacgcgcg cttcgtttgc gttttataat acaatggcgg aggtcgatgc ttttattgac
     7621 gcccttcgcg ctgtacaaaa gatttttgcg taatgaattc gaaatcgctt tatcaggaag
     7681 ttatcctgga tcacaacaag aagccgcgca actatggcac gttggatcat gcgagccatc
     7741 atgcagtcgg gcataacccg ttatgcggcg accatctcga tattgcgctt catctcgaag
     7801 gtgaacgcat tgatcagatt gccttccacg gtgagtcatg cgccatctgc aaggcttcgg
     7861 catcgatgat gaccacggct gtcaaaggaa agacgtcggc tgatgctgaa accttgatac
     7921 aggaatttcg ggatatggca gtgggcaaac tcaatctcga agctccgaat cacttgggcc
     7981 ggttaaccgt attcgcagga gtgcgtgacc ttccgacgcg cgtgaaatgc gcgattcttc
     8041 cttggcacac gctccatgcc gcgcttcact ccgttacgac gacctcgacc gaggccgaag
     8101 atgatccgat gcacgccgcg atcggcgatg cctgatccga tcaggaggac tcatgcccaa
     8161 aatagctgaa atagagggta cgccaaatcg caacgctttg aagtttatcc tcagggaacc
     8221 gttgacttgg ggtgttaccc gctcatatga caacgcagag caggcaaagg acgacccgct
     8281 ggccgcggcc ctgttcgaaa tcgatcacgt aaccaacgta ttttatgttg atcgatggat
     8341 taccattacc caggatggcg gcgcagactg gcaagacctc gcgcgcgagg tcgccgaccc
     8401 catccgggcg gcgccggctg cagacaggca atccgcagcg acggttgcgg ccgcgaacac
     8461 ggccatggcg gggctaagcc cggaagacca ggagcgtctt gaaaccatca acgccttgct
     8521 cgacgaagag gttcggcctt acctccaaaa cgacgggggc gacctgcatg tgctcagcct
     8581 tgaaggcaac aagctcagcg tgcactacca gggtgcatgc ggcacttgcc ccagctcgat
     8641 taccgggacg ctcaggggta ttcagaatat gttgaggtcg atcgagccgg acatcgagat
     8701 cgtcgcgctc tgaagcttga aattatctat tctggctaaa aatcccgaac aggattagta
     8761 tcgcggcagg cggttatatc tgccgaggat cgcgcttgcc gttcggtcac tatcgaaccg
     8821 gcccaaaaaa agaaacaccc catgctctgg atcaaatccc tgcacatcat ctttg
//
LOCUS       DASSHJ010000276         8952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1368097,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000276 DASSHJ010000000
VERSION     DASSHJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368097"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFI43_10560"
     CDS             complement(<1..542)
                     /locus_tag="VFI43_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7062608.1"
                     /translation="MSTLKSILHETRVFPPDPAFVKQANVSGMEAYRALCNEAESDLP
                     AFWARLAREHIIWQKPFTRVLDESNHPFFKWFDDGELNVSFNCLDRHLATQPDKIAII
                     FEADDGTITRVTYRDLHGQVCRFANGLKSRGIRKGDRVIIYMPMSIEAVVAMQACARI
                     GATHSVVFGGFSAKSLHERIT"
     gene            831..1259
                     /gene="rimP"
                     /locus_tag="VFI43_10565"
     CDS             831..1259
                     /gene="rimP"
                     /locus_tag="VFI43_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET7062609.1"
                     /translation="MNLYELLDSTLSGLGYELVDVERISRGRLLRVFMDKPGGISVDD
                     CVTVSNHLSRLFAVENIDYERLEISSPGLDRPLKKVSDFIRFAGEKVRLKLRVGLQGR
                     RNFVGIVREVNDGVLKLEVDGKILDFALNNLEKARLVPKL"
     gene            1289..2761
                     /gene="nusA"
                     /locus_tag="VFI43_10570"
     CDS             1289..2761
                     /gene="nusA"
                     /locus_tag="VFI43_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET7062610.1"
                     /translation="MTREVLLLVDALAREKNVEKGIVFVALESALASATKKRFHEDAD
                     VRVSIDHQTGDYQSLRRWHVVPDDAVEDPARQIGLSEALLRNPETKQDEFIEEVLEPV
                     EFGRIGAQAAKQVIFQKIRDAEREQILNDFLDRKEFMVTGTIKRMERGSAIVESGRVE
                     AVLPRDQMIPKENLRVGDRVRAYLHKVDRGARGPQLVLSRIAPEFLIKLFELEVPEIE
                     EGLLEIKVAARDPGSRAKIAVKSNDQRVDPIGTCVGMRGSRVQAVTGELAGERVDIIL
                     WSEDPATFVINALAPAEISSILVDEEKHSMDIVVDEDNLAQAIGRGGQNVRLASELTG
                     WELNIMTMEESQAKNEEEFSILRQLFMEKLDVDEEVADILVQEGFTTLEEVAYVPLNE
                     MLEIEAFDEQTVNELRTRARNALLTEAIVTEEKMEHIAEDLLSLEGMDSQTARELAAK
                     GVNTQEDLADLAVDDLVEMIEMDPDKARQLIMAARAPWFA"
     gene            2798..5419
                     /gene="infB"
                     /locus_tag="VFI43_10575"
     CDS             2798..5419
                     /gene="infB"
                     /locus_tag="VFI43_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7062611.1"
                     /translation="MAQMNVEQFASELGVLPAVLLEQLRAAGVSKRQTEDNLTETDKT
                     QLLEYLRRIHGAREEKGKISLPRRQTSEIKKADSTGKPRSIQVEVRKKRVIVRSDSGD
                     KMTPAAKKVEPAPPPAQPPVTAPVLDPAQLALRQEEARRHAELIARQTAEIREKKLRQ
                     KLAGTETKEPEPAPSPAGGPVPPAPVEAPAGVAVPPVEATTAAPEAQPPGSTTEGTLH
                     KPVVKPEDKAAKAKKKQTKQVVWKDDRVEKRGLKTRGDLAGAKGWRGRKDKNNKTGAE
                     DQGAHSFFAPTEPIVHEVMVPETISVAALAQKMSIKAAEVIKALMKMGNMVTINQMLD
                     QETAMIVVEELGHVAKYATLDSPEAFLADTELPTPGTGTEPRAPVVTVMGHVDHGKTS
                     LLDYIRRTRVASGEAGGITQHIGAYHVETDRGMVTFLDTPGHEAFTAMRARGAKVTDL
                     VVLVVAADDGVMPQTVEAIHHAKAAKVPIVVAVTKIDKPEANPERIRQELVAHQVVPE
                     DWGGDTMFAEVSAKTGQGIDSLLESVLLQAEVLELSAPVDASARGIVIESRLDKGRGP
                     VATILVQSGTLKRGDVLLAGAVFGRVRAMLDENAKAVEKAGPSIPVEIQGLSEVPVAG
                     EAVVALADERKAREIALFRQGKFRDVKLARQQAAKLENVFEQKGEVKVLPLIIKADVQ
                     GSYEALTHALQRLSTDEVKVNIIHSGVGAITESDINLALASKAVVIGFNSRADSVARK
                     LIASSGVDVRYYSIIYEAVDEIKAALSGMMAPDRRENITGLLEIREVFRISRVGVVAG
                     CYVLEGIIKRTSFIRLIRNGQLIHTGELDSLKRFKDDVKEVRAGFECGLSLKNYNDIQ
                     TGDQIETYEIVEVARSL"
     gene            5467..5847
                     /gene="rbfA"
                     /locus_tag="VFI43_10580"
     CDS             5467..5847
                     /gene="rbfA"
                     /locus_tag="VFI43_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7062612.1"
                     /translation="MPKDYPRALRVADQIQRELSDLIRNEIKDPRIGMITLTGVEVSH
                     DYSNAKVFYTTLGSASDNFLTANGLEHAAGFLRSHLSHRLKLRVIPQLHFIYDESVER
                     GVRLAHLIDEAVAGNHAPKEPDNS"
     gene            5860..6834
                     /gene="truB"
                     /locus_tag="VFI43_10585"
     CDS             5860..6834
                     /gene="truB"
                     /locus_tag="VFI43_10585"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7062613.1"
                     /translation="MKPRSVPSRRFDREISGVLLLDKPSGISSSQALQIARRMFAASK
                     AGHTGTLDPMATGLLPVCFGEATKFSSLLLGADKTYEATLRLGYTSTTGDAEGEISPM
                     LGRACGEALTLSHLHEVLQTFTGTSLQIPPMYSALKHKGKPMYLYAREGIAVDREPRE
                     IFIHELRGEAASNDEVRIVVACGTGTYIRTLAEDIGRALGYGSAYLTALRRTASGPFR
                     VSDAITLGAIKNMSTGERDHCVLPVDRLALGLPAVNLNETAAAFLKQGRTVEDYACES
                     SLREAETVRVYDEENHFLGLGEVTALHAIAPKRLINPSHCLDKPSTPC"
     gene            complement(6906..7793)
                     /locus_tag="VFI43_10590"
     CDS             complement(6906..7793)
                     /locus_tag="VFI43_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7062614.1"
                     /translation="MISKPDILNATILIVDDQDANVRLLEQMLSDAGYAQVASTMNPR
                     EVCDLYRDNRYDLILLDLQMPGMDGFQVLEGLKQIETHGYLPVLVITAQPGHKLRALQ
                     AGAKDFVSKPFDLVEVQTRIHNMLEVRLLYKKLKNYNEELERTVAERTAELRESEARF
                     RRLTELASDWYWEQDEKGQFTKVYGPVLEMLGITVDTLLGEPKGNKGANWNDAEYEAF
                     KSNIASRRPFLDFVYTRIRSDGSHQYLMVSGEPMFDSSGRFTGYRGIGKDVTESIQSK
                     HNSDLPLTPPQTSAPTKSG"
     gene            complement(8011..>8952)
                     /locus_tag="VFI43_10595"
     CDS             complement(8011..>8952)
                     /locus_tag="VFI43_10595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7062615.1"
                     /translation="YMVSESEIREAKILIVDDQEQNVQLLEHMLAAANYTCVTSTTDP
                     FKVHELHKKNRYDLILLDLKMGGMDGFQVLDALREIESDGYLSVLVITANPDAKLRAL
                     QSGAKDFVSKPFDMAEVLARVHNLLEVRLLHQKARNHAKTQEFRALHDPLTGLANRRL
                     LVERVSQAIIHAKRNDGIMAVVYLDLDGFRQINNTMGHDAGDLLLKLVAARLVAAVRQ
                     EDTVARPGGDEFVIAMPHVNSIEGVELTAEKVIKVLSQPYNLEGSEVSVTASAGVGLY
                     PLHGKDVNTLLKNADAALLEAKQANKNTYRFSVRVNV"
BASE COUNT         2061 a         2284 c         2585 g         2022 t
ORIGIN      
        1 gtaatgcgtt catgcaggct ttttgccgaa aagcctccaa acaccaccga atgtgttgcg
       61 ccgatgcgcg cgcaggcttg catcgcaacc acggcttcaa tgctcatggg catgtagata
      121 atgactcggt ctcccttgcg gatacccctc gatttgagtc cattggcaaa cctgcatacc
      181 tgcccatgga ggtcccgata agttactcga gttatggtgc cgtcatccgc ttcgaagatg
      241 atggcaattt tgtccggctg ggtggcaaga tgacggtcga ggcagttgaa ggagacgttc
      301 agttctccat cgtcgaacca tttgaaaaaa gggtgattgc tttcatccag cactcgggtg
      361 aaaggttttt gccagatgat gtgctcccgc gccagtctgg cccagaaagc gggcaaatcg
      421 ctttctgctt cgttgcagag ggcacggtaa gcctccattc ccgacacgtt ggcctgcttc
      481 acaaaggcgg ggtcgggcgg gaatactcgg gtttcatgca ggatggattt gagggtggac
      541 acgatctctc cgttttttgc tggggatgtt ttggcgaatt gtacatccta tttgccgagg
      601 tttcccggct ccgtgccggg ccctcgctat tttccttcag gttcacggta cggccgaccg
      661 agggattgtt gtttcctgtg agacttctcc gcgggctcct ttttgcgcag cttgagtggc
      721 cgctacaaac tattattcat cactatctat ttcaataaat ataaattttt gctaaaatag
      781 cgataactat gggctctttt cagcccattt tttgtttgtg tgccggcgcc atgaatctgt
      841 acgaattgct tgactctacc ttgagcgggc ttggttacga actggtagat gtggaacgga
      901 tttcgcgagg ccggttgctc cgggtgttca tggacaagcc tgggggcatc agcgtggacg
      961 actgtgtaac ggtgagcaac catttgagcc ggttgttcgc ggttgagaac attgattacg
     1021 aacggctgga aatctcctcg ccggggctcg acaggccgct taaaaaggtt tctgacttta
     1081 tccgcttcgc gggagagaag gtcagactta agctgcgcgt cgggttacag ggccggagga
     1141 attttgtcgg gattgtgcgc gaagtgaatg acggagtgtt gaagctggaa gtagatggaa
     1201 aaattctcga ttttgcgttg aataatcttg aaaaggcccg tttggttccc aaattataat
     1261 cgtcctggaa atataggctg gaggaaagat gacccgcgag gttttgctgt tggtggatgc
     1321 attggcgcga gagaaaaatg tcgagaaagg catcgttttt gtcgcactcg aatcagctct
     1381 ggcatccgct actaaaaagc gttttcatga ggacgctgat gtgcgggtgt caattgacca
     1441 ccagaccggt gattaccagt ccctgcggcg ctggcacgtc gttcccgacg atgccgtgga
     1501 ggatcctgcc cgccagattg gattgagcga agcattgctt cgcaatcccg agaccaagca
     1561 ggatgagttt atcgaggaag tgctggagcc tgtcgaattt ggtcgcattg gcgcgcaggc
     1621 cgcgaagcag gtcatattcc agaaaatacg cgatgccgag cgtgagcaga ttctcaatga
     1681 ttttctcgat cgcaaggaat tcatggttac cgggacgatc aaacgtatgg agcgaggtag
     1741 cgcaattgtc gaatcgggta gagtcgaggc agtgttgccg cgagatcaga tgattccgaa
     1801 ggaaaatctc cgcgtggggg atcgcgtacg tgcctatctg cataaggtgg accggggcgc
     1861 gcgcggaccg caattagtac tgtcgcggat cgcgccggag ttcctgataa agctttttga
     1921 acttgaggtc ccggaaatcg aggagggctt gctggagata aaggtagcgg cgcgggaccc
     1981 ggggtcccgg gcaaagatcg cagtcaaatc gaacgaccag cgggtcgatc cgatcggtac
     2041 gtgcgtaggc atgcgaggtt cgagggtgca ggcggtcacg ggcgaactgg ctggagaaag
     2101 ggtggacatc atcctctggt cggaggaccc cgcgactttc gtgatcaatg cgctggctcc
     2161 ggcagagatc agcagcattc tcgtcgatga ggaaaagcac agcatggata tcgtggtgga
     2221 tgaggacaac cttgcccagg cgattggacg cggtggtcag aatgtccggc tcgcttccga
     2281 gttgacgggc tgggaactca acatcatgac catggaggaa tcccaggcta aaaatgaaga
     2341 ggaattttca atacttcgtc agctctttat ggaaaaactc gacgtagacg aagaggtcgc
     2401 ggacatcctc gttcaggaag gatttactac actggaagaa gtagcctatg ttccccttaa
     2461 cgaaatgctg gaaatcgagg cgtttgacga gcagacggtc aacgaactgc gaacccgggc
     2521 gcgtaacgcc ctgctgaccg aggccatcgt gacggaggaa aaaatggagc acatagcgga
     2581 agacctgcta tccctggagg gaatggacag ccagactgct cgggagcttg ccgccaaggg
     2641 cgtgaatacg caggaagacc tggctgatct ggcggttgat gatctggtgg aaatgatcga
     2701 gatggacccc gataaggccc ggcagttgat catggctgca agggccccat ggtttgcttg
     2761 attgaaacat cgaagcgaag cattacaaag gtaattaatg gctcaaatga acgtagaaca
     2821 gtttgcaagt gaactcggtg tcctgccagc ggtgctgctg gagcagctca gggctgctgg
     2881 cgtgagcaaa cgccagaccg aggacaatct gaccgaaacg gataagacgc agcttctcga
     2941 gtatttgcgg agaattcatg gtgccaggga ggaaaaagga aagatttccc tcccccgccg
     3001 ccagacttct gaaatcaaga aggctgacag caccggaaag ccgcgcagca tacaggtcga
     3061 ggttcgcaaa aagcgcgtta ttgtcaggag cgactcgggc gacaagatga cgcctgctgc
     3121 caaaaaggtc gagccggcgc ccccgcctgc gcagccgccg gttacagcac cggtactcga
     3181 tcccgcacag cttgcgctgc ggcaggagga agcaagaagg cacgctgaac tgattgcgcg
     3241 gcagactgcc gagatcagag aaaagaagct gcggcaaaaa cttgcaggga ccgagacaaa
     3301 ggaaccggag cctgctccct cgcctgcagg agggcccgtg cctcccgcgc cggtggaggc
     3361 gcctgctggc gttgctgttc ctccggttga agccacgacc gctgctccag aagcccaacc
     3421 accgggttct acaactgaag gcacgcttca taagccggtt gtcaaacctg aggataaggc
     3481 agccaaggcg aaaaagaaac agaccaagca ggtcgtctgg aaggacgaca gagttgagaa
     3541 gcgtggcctc aagacacggg gcgatctcgc gggtgccaaa gggtggcgtg gacgcaagga
     3601 taaaaataat aaaacaggtg ctgaggatca gggcgctcac agctttttcg cgcccaccga
     3661 gccgatcgtt catgaagtga tggtgcctga aacgatttcc gtggctgcgc tcgcccagaa
     3721 gatgtccatc aaggccgcgg aggtcatcaa ggcgctgatg aaaatgggca atatggtgac
     3781 gattaatcag atgctcgacc aggaaaccgc gatgatcgta gtggaggaac tcggtcacgt
     3841 cgcgaaatac gcgacgctcg acagtcccga ggccttcttg gctgatacgg aattacccac
     3901 tcctgggacc ggcaccgaac cgcgcgcacc tgttgtcact gtcatgggtc acgtggacca
     3961 tgggaagacc tctcttctcg attacattcg tcgcacccgc gtggcaagtg gcgaagcagg
     4021 cggtattacc cagcatatcg gtgcgtatca cgttgaaacc gatcggggta tggtgacgtt
     4081 tctcgacact cccggccatg aggcctttac tgcaatgcgt gcccgtggcg caaaagtgac
     4141 ggatcttgtc gtgctggtcg tggcggcgga cgatggcgtg atgccccaga cagtcgaggc
     4201 cattcatcac gccaaagcgg caaaggtccc gattgttgtt gcggtaacga aaatcgacaa
     4261 accggaagcc aatcccgagc gtatcaggca ggaactcgtg gcgcaccagg tggtacctga
     4321 agattggggc ggcgatacga tgttcgcgga ggtttccgca aagaccgggc aaggaatcga
     4381 ctcgcttctg gaaagcgtgc tgcttcaagc ggaagtgctg gagcttagcg cacccgttga
     4441 tgcatcggcg agaggaattg tcattgaatc gcgtctggac aaggggcgag gcccggtagc
     4501 gaccatactg gtgcaatcgg gaacgttgaa gcgcggtgat gttctcctgg ctggcgccgt
     4561 attcggacga gtccgggcga tgctcgatga aaacgcgaag gcggtggaaa aggcagggcc
     4621 ctcgataccg gttgagatcc agggtctgtc tgaggtcccg gtggctgggg aagcggtcgt
     4681 tgcgctggct gacgagcgca aggcccgtga aatcgcgctc ttccgtcaag gaaaattccg
     4741 tgatgtgaag ctggccagac aacaggctgc caagcttgaa aatgtgttcg agcagaaggg
     4801 cgaggtcaaa gtgttgccgc ttatcatcaa agcggacgtg cagggttcat acgaggcact
     4861 gacacatgcc ttgcagcggc tttccaccga cgaggtcaag gtcaacatta tccacagcgg
     4921 cgtgggcgcg attacggagt ccgacattaa cctggctctg gcttccaagg cggtggtgat
     4981 cgggttcaac agccgtgccg attctgttgc gcgcaagctc attgcctcca gcggagtcga
     5041 tgtgcgctat tacagcatca tctatgaggc ggtggatgaa atcaaggcgg cgttgtcagg
     5101 catgatggcg cctgaccgcc gggaaaatat cactggtttg ctggagatcc gggaagtctt
     5161 caggatttcc agggtgggag tggtcgcagg ttgctacgtg ctggaaggaa ttatcaaacg
     5221 gacgtcattt atccggctga tacgcaacgg gcaattgatc cataccggcg aactggattc
     5281 attgaagcgc ttcaaggacg atgtcaagga agtgcgcgca gggttcgaat gcggactatc
     5341 gcttaaaaac tacaatgata tccagacggg tgatcagatc gaaacgtatg aaatcgttga
     5401 agtggcgcgc agcttgtaag ggcacgcgcg aagggcctgt taacaggccc tattacagga
     5461 actatcatgc ctaaggatta tcctcgtgcg ttgcgcgttg ccgatcagat ccagcgtgag
     5521 ttatcggatc tgattcgcaa cgaaataaag gatccgcgta tcgggatgat caccctcaca
     5581 ggcgtggaag tgagtcacga ctatagcaac gccaaggttt tttatacgac tttgggaagt
     5641 gcgagcgata attttctgac ggcgaacggg ctggaacacg cggcgggctt cctgcgcagc
     5701 cacttgtcgc accgcttgaa gcttcgggta atacctcaac tccatttcat ctatgacgag
     5761 tctgtcgagc gaggcgtgcg tcttgcgcat ctcatagatg aggctgtggc ggggaaccat
     5821 gcgcccaaag aaccggacaa ctcctgattc atctccatat tgaaaccccg tagcgtgcct
     5881 tcccggcgct ttgaccggga gatcagtggt gtgcttctgc ttgacaagcc gtcaggaatt
     5941 tcttccagcc aggctctcca gattgcaaga cggatgttcg ctgccagcaa ggcgggccat
     6001 accggtaccc tcgatcccat ggcaacggga ttgttgccgg tctgctttgg tgaggccacc
     6061 aagttttcct cattgttgct cggcgccgat aaaacttacg aagcgacgct gagattaggc
     6121 tataccagca caacgggaga tgccgaaggc gagatttcgc cgatgctagg gcgagcctgt
     6181 ggggaggcat tgacgctttc acacctacat gaagtgctgc aaacttttac cggcaccagc
     6241 ctgcagattc caccgatgta cagtgctctc aagcataaag gcaaacctat gtatctttat
     6301 gcacgcgagg gcatagccgt cgatcgtgag ccccgcgaga tcttcataca cgagttgcgc
     6361 ggggaggctg cttcaaatga tgaggtgcgt atcgtagtcg cgtgcgggac aggtacttac
     6421 atacgaacgc tggcggaaga tattggccgt gcacttggat acggcagtgc atatctgact
     6481 gctttgcggc gtacagcctc ggggccgttt cgagtgagcg acgcgattac gctgggggca
     6541 atcaagaaca tgtcgaccgg tgagcgcgat cattgcgtgc tgcctgtcga tcgtcttgcc
     6601 ctgggattac cggcggtcaa cctgaacgaa acagctgcag ctttcctgaa gcagggccgc
     6661 acggtcgagg attacgcatg cgaatccagc ttgagggaag ccgagacggt gagggtgtat
     6721 gatgaggaaa atcattttct aggtctgggg gaagtcacgg ccctgcacgc aatagcgccg
     6781 aagcgcctca taaatccgtc acactgcctc gataagccat ccaccccatg ctaacggaag
     6841 caaaaacgcg ggtgattcga ggctcctgta cgatgcgcac ccgccacgtt catccggtcg
     6901 cacaatcacc cggattttgt cggggcggag gtttgggggg gagttaacgg aaggtctgag
     6961 ttatgcttcg actgaatgct ttccgtgaca tccttgccta taccacggta accggtaaaa
     7021 cggccggacg aatcgaacat gggttcaccg ctcaccatca gatattgatg cgacccgtct
     7081 gatctgatgc gagtgtagac aaagtcgaga aaaggccgcc tcgaggcaat attcgacttg
     7141 aacgcctcat actcggcatc attccaattc gctcctttat tccctttggg ttcccccagc
     7201 aaggtatcga cggtgattcc gagcatttca agaacgggac catatacttt cgtaaactgg
     7261 cccttttcgt cttgctccca gtaccagtcg gacgccagct ccgtcaagcg gcgaaagcgc
     7321 gcctcgctct cgcgtagttc tgcagttcgc tcagcgaccg tgcgctccag ttcctcgtta
     7381 taatttttaa gctttttata tagcaggcga acttcaagca tattgtgaat ccgggtctgg
     7441 acttcgacga gatcgaatgg cttgctcaca aaatcctttg cgcctgcttg cagcgcgcga
     7501 agcttatgcc ccggttgggc ggtgatcacc agcacaggga gatagccgtg cgtttcgatc
     7561 tgcttcagcc cttccagcac ctgaaagcca tccattcccg gcatctgcaa atcaagcagt
     7621 atcagatcgt atctgttatc ccggtataga tcacatacct cccgcggatt catcgttgac
     7681 gctacttgcg cgtaacctgc atcacttaac atctgctcaa gcaagcggac atttgcgtct
     7741 tgatcatcaa caatcaggat cgtcgcgttg agaatgtctg gtttgctaat catttattcc
     7801 agccctgcct tctttcaatt gatcaatttc ttaagtattt atactatacc catttgcatg
     7861 tataggtgac atgcaaaagt ggttaatatg cttttcaatt ttcattaact gatctgatgg
     7921 cttggcggtt ctgcctaccg ctgctttcag caaaatcgcg gctccccaaa gcttccgggt
     7981 aaaagctcct aaagtgaaat aaagagaaat tcagacgttc actcgtaccg aaaaccggta
     8041 tgtattcttg tttgcctgct ttgcctccag cagagcggca tccgcattct tcaacagcgt
     8101 gtttacatct ttgccgtgaa ggggataaag tcctacacct gcacttgctg tcacggaaac
     8161 ctcgcttcct tcaagattgt aaggttgcga gagcactttt attacttttt ctgccgtcag
     8221 ttccactccc tcaatgctgt ttacgtgcgg catcgcgatc acgaactcgt caccccccgg
     8281 gcgcgccacc gtatcctctt ggcgcactgc tgctacaagg cgcgcggcaa cgagttttag
     8341 aagcaggtca cctgcatcgt gtcccatggt gttgttgatc tgccggaatc catccagatc
     8401 caggtatacg accgccataa tgccgtcgtt tctttttgca tggataattg cctgcgacac
     8461 tctttccacc agaagcctcc ggttggcgag ccccgtcaag gggtcatgca acgctcggaa
     8521 ttcctgcgtc ttcgcatgat tgcgcgcctt ctggtgaagt aaccgcactt ccagcaggtt
     8581 atgaacgcgg gcgagcactt cagccatatc aaacggttta ctcacgaagt ctttcgctcc
     8641 ggactggagc gcgcgaagct tggcatccgg gttcgctgtt ataaccagga cggaaagata
     8701 tccgtccgac tcgatttccc tgagcgcatc cagtacctga aacccgtcca ttccccccat
     8761 tttcaggtcg agcagaatca gatcgtagcg attcttttta tgcaattcat gaaccttgaa
     8821 cgggtcggtc gtggaggtga cgcaggtata gtttgcagcg gcaagcatgt gttccagcaa
     8881 ctggacattt tgttcctgat cgtcaacgat caggattttc gcctcacgaa tttcggattc
     8941 actgaccata ta
//
LOCUS       DASSHJ010000277         6555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_605680, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000277 DASSHJ010000000
VERSION     DASSHJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605680"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFI43_10600"
     CDS             complement(<1..431)
                     /locus_tag="VFI43_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062616.1"
                     /translation="MGRHYSTRDFFRQMPNRLLARYFEAHDVLAGLDFAAMKGIHIEP
                     LFEAWLELPEGQRNAMDADFSEIHALSGEKGWCAIRDEAQWQFREKPDAFTAFVDAMG
                     SLDDHSERAMTTFLEYPQFWKGATLFCHADTLPYWRKRKGFP"
     gene            625..1644
                     /locus_tag="VFI43_10605"
     CDS             625..1644
                     /locus_tag="VFI43_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062617.1"
                     /translation="MLEQFVKRIAETTRRAKSEASLQIKLETILAELLSLYGIDYQPS
                     VNETLSSQGLSQVDSTRPDSLFGHVVLDYKAPGLLKNTKELRKAKRQITGYLDNVTAK
                     DRVTEAQKWAGILWDGASITFCHSDGRGWQWSGPFPVSEASLLRLIGNYRALKRKPLT
                     ARLLAQSFGKESDVARAAITTMCDHLSKPRHRTTMLYREWKRLFEQVSTFGLDQLPAL
                     KAWAASLGIATKDASQILFAIHSYYALVVKLLTSELLTTINPVAVESICQQMANSSVA
                     RLYQLLADLENGNYWHSLRISNFLEGDFFSWYTNERSNALAAAIQEVAAELLQFEPAT
                     SLIKP"
     gene            1657..3852
                     /locus_tag="VFI43_10610"
     CDS             1657..3852
                     /locus_tag="VFI43_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HET7062618.1"
                     /translation="MLKEFYTTLVDEQIRHDLGEYYTPDWLAQRVLDSVHYEGDLDST
                     VLDPSCGSGTFLLECINRIRARADAAGTERQKLLRILLSRVKGMDLNPLAVISARANF
                     ILAAADLIFSVGDDLELPIYLADCINVPVKSRAEDGLEVLTFPLDTELGRKTFEIPNI
                     LIETKVLGKVLLSCEDAINEGRSESSFLAAIRGISAVKQNLDVRVESRLSNLFQIVSG
                     LEQQKWNRIWCRIIKNSFSPNGFIDQVDFLVGNPPWVRWSRLPVFYRERVKDFCNYYG
                     LVSGRGYTGGIETDISTVVLYSAADHWLKRGGKIGLLITWTVFKSGSARGFRLGSLPG
                     DAGLRMTALSDLRSIQPFPDATNETSIYLATKVGATKDAKFKSIPTETWTPKGSGRIN
                     PRASLTSVLDSVSITQGFAFPVSDWGSPLWTGDKSRFDESRNLQGRSPYLDNAHRGTI
                     SDLARVYWVKVEKYASETNRALIRTLTVDEFSGATEIEPTKAAWIEADLLHPLIRGRD
                     LGRYCFETDQWYQIIPNTHYENVDDEETFAATRPLAFAYFSKYKELLLKRSTYRRFQK
                     HLPFYVIYCVGDYSFKKWKVAWMEQQDPASFRCAVVADQPPSALPHKVVVPDHKLYFI
                     DAESKNEAHYVAAYLNSHPVRTWLGGFLHGKQIATTIFEFMRVPKFDPEDPNHVRISE
                     ISLQAHKTRTKNFSTKPLSEEYEKELAELVRAVAWTRQTGSASFVKGKP"
     gene            4088..4315
                     /locus_tag="VFI43_10615"
     CDS             4088..4315
                     /locus_tag="VFI43_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7062619.1"
                     /translation="MQAELERLRAENAQLKSKGKGGISLKVSEKGAVSLYGMGRFPVT
                     LYKEQWLRLLASAAEIEAFIRENEPQLKTKE"
     gene            complement(5176..6474)
                     /locus_tag="VFI43_10620"
     CDS             complement(5176..6474)
                     /locus_tag="VFI43_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015137 - citrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015746 - citrate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate:proton symporter"
                     /protein_id="HET7062620.1"
                     /translation="MLAILGLAAIVLMLVTILTRRLSPIVALITFPVLASLAGGFGGK
                     TAIFIGHGIQNVAPVAAMFIFAILYFGVMTDAGMLDPIIDRILRAVGLSPSRIVVGTA
                     LLALLAHLDGSGAVTFLVTIPVMLPLFDRLKMDRRVLACAAALAAGVNFLPWTGPMIR
                     ASAALHIPITAIFNPLAPVQLVGLVYVFAVAWWMGAREARRLGLNQKGKATEAFIRHL
                     TEEELQLRRPRCFWFNLILTIGLIVAMVGLRLEPATVFMCGTVLALTVNYPNLKQQAE
                     RIDAHARAALLMAAVLFAAGAFTGILGGTGMLTAMAKSVASHVPVRMAGQMPFVLGLV
                     SMPLSLIFDPDSFYFGLLPVLAGAGKVLGVAPVHMAQAAVLGQMTTGFPISPLTPATF
                     LLVGLAGVELGDHQKFAIPYLFAASVVMTIAAVAFGVIPI"
BASE COUNT         1557 a         1845 c         1760 g         1393 t
ORIGIN      
        1 ggaaagccct tccgcttgcg ccagtaaggc aaggtgtccg cgtggcagaa aagcgtagcg
       61 cccttccaga actgcgggta ttccagaaac gtcgtcatcg cccgctcgga gtgatcgtcc
      121 aggctgccca tcgcatccac gaaggccgtg aacgcatccg gcttttcccg gaactgccac
      181 tgagcctcat cccggatcgc gcaccagccc ttttcaccgc tcagcgcgtg aatctcgctg
      241 aagtccgcat ccatcgcgtt gcgctggcct tccggcaatt ccagccatgc ttcaaacagc
      301 ggctctatgt ggattccctt cattgccgca aaatccagcc ctgccaatac gtcatgcgcc
      361 tcgaaatatc gcgccagcag ccggttcggc atctgccgga aaaagtctcg cgtggagtag
      421 tgacggccca tatcccctcc gccatcagcg caaacccggc gcgctctggg acatagtagg
      481 atgttgctgt actcaacaat agcgcagatt tgcatcatga gagcagcatt ctgacgttgc
      541 cggagccagt caccaatctt tgcggggcct tttgagaccg cattcaccag agtcaccgcc
      601 gaagccgaag gtttttcttc catcatgctc gaacagtttg tcaagcgcat tgccgaaacc
      661 acgcgccgcg cgaaaagcga ggcatcatta caaatcaaac tggaaaccat tctcgcggag
      721 ttgctctccc tctatggcat tgactaccaa ccgtccgtca atgaaactct gagcagccaa
      781 ggattatcgc aggttgattc gacacggcca gatagccttt tcggtcacgt tgtcctggac
      841 tataaagcgc cgggccttct caaaaacacg aaggaattac gcaaggcgaa aagacaaatc
      901 acgggctatc tcgacaacgt taccgccaaa gaccgcgtga cggaggctca gaagtgggcg
      961 ggaatccttt gggacggcgc gtcaatcacg ttctgccatt cagatggacg aggttggcag
     1021 tggagcggcc cattcccggt ttccgaagcc tcccttctga ggctaattgg aaactaccga
     1081 gcactgaagc gtaagcccct aaccgcacga ttgctggcgc agtcttttgg aaaagaatct
     1141 gatgttgccc gcgctgcgat aacaacaatg tgtgaccacc tcagcaagcc ccgacaccgc
     1201 accaccatgc tttaccggga atggaagagg ctctttgagc aggtatccac atttggacta
     1261 gaccaacttc ccgccttgaa ggcttgggcc gcctctttgg gaattgcgac aaaagacgcc
     1321 tctcagattc tctttgcgat tcacagctat tacgcgctcg ttgtgaaact gcttacctcc
     1381 gagcttctca caacgattaa cccggttgcc gtggaatcca tttgtcaaca gatggcaaat
     1441 tcctccgtgg cgcgactgta ccaacttctt gcggacctgg aaaatggaaa ctattggcac
     1501 agtctccgca taagcaactt tttggaaggg gacttcttta gctggtacac gaacgagcgg
     1561 tcaaacgctc tcgctgccgc cattcaggaa gttgcggcgg agcttttgca attcgagcct
     1621 gcaacgtccc taataaagcc ctaggcaatc agagatttgc tgaaagagtt ttacacgacg
     1681 ctagtagacg aacaaatccg gcatgacctg ggcgaatact acactcctga ttggcttgca
     1741 cagcgcgtac tagattcggt ccactatgag ggtgacctag attcaacggt gcttgatccc
     1801 tcatgcggct cgggcacatt cctcttggag tgcatcaatc gaatacgtgc gcgggccgat
     1861 gcggcgggga ctgagcgcca aaagctcttg cgcatacttc tgagtcgcgt caagggaatg
     1921 gacctcaacc ccctcgccgt catctctgcc cgcgccaatt tcattttggc cgctgctgat
     1981 ttgattttca gtgtgggcga tgatctggag ttacccatct accttgcgga ctgcatcaat
     2041 gttcccgtca agtccagagc cgaagatggc cttgaagtgc ttaccttccc gctggacacg
     2101 gagcttggcc gcaagacatt tgagattccg aacattctta tcgaaacaaa ggttctcggg
     2161 aaagtcctat tgtcttgtga ggatgcaatc aacgaagggc gctccgaaag ctctttcctc
     2221 gcagctattc gaggtatttc cgctgtgaag caaaatcttg atgtgcgcgt ggaatctcgc
     2281 ttgtcaaacc tgttccaaat tgtttcgggt ttggagcaac agaagtggaa tcgcatttgg
     2341 tgccgcatca taaagaacag cttttccccg aacggtttca tcgaccaagt ggattttctt
     2401 gttggcaatc caccatgggt tagatggagc cgcctacccg ttttttatcg tgagcgcgtc
     2461 aaggactttt gcaattacta cggccttgtt tcaggcagag ggtacactgg cggaatcgaa
     2521 accgacattt caaccgtggt gctatacagc gcggcggatc actggttgaa gcgcggcggc
     2581 aaaatcggtt tgctaattac ctggacagta ttcaaatctg gttcagcgcg gggattccgg
     2641 ctcgggagcc ttccggggga tgcagggctg aggatgacgg cactgtctga tcttcggagt
     2701 atccagcctt ttccagatgc cacaaatgaa acctccatat atcttgcgac aaaggttggc
     2761 gcaacgaagg atgcaaagtt caagagcata cccactgaga cgtggacacc gaaagggtct
     2821 ggccggatca acccgcgtgc aagtctcact tccgttctag acagcgtgtc aatcacccaa
     2881 ggatttgctt tccccgtctc cgattggggt tcaccacttt ggacaggtga taaaagtcgc
     2941 ttcgatgagt cgcgtaattt gcaaggacgt tcaccgtact tggacaacgc gcaccgggga
     3001 accatatccg atcttgcaag ggtttattgg gtcaaggttg agaagtatgc ctccgaaacg
     3061 aatcgggcgc tgataagaac gttgactgtt gatgagtttt ccggagccac tgagattgaa
     3121 cccacgaaag cggcatggat tgaggctgat ttgctccatc cgctgattcg tggccgcgac
     3181 cttgggcgct attgcttcga gactgaccag tggtatcaga tcattccaaa cacgcactac
     3241 gaaaacgttg atgatgaaga gacattcgcg gcaacgcggc cgcttgcctt tgcgtacttc
     3301 tcgaaataca aagagcttct tttgaagcgg tcaacgtacc ggcggtttca aaaacacctg
     3361 cccttctacg tcatctactg cgtaggtgac tacagcttca agaaatggaa agtggcttgg
     3421 atggagcagc aagaccccgc gtcatttcgc tgcgcggttg tggcggatca acccccttca
     3481 gcgttacccc acaaagttgt tgttcccgat cacaagctct atttcatcga cgcagagagc
     3541 aagaacgaag cgcattacgt ggctgcatat ttgaactctc atccggttcg cacttggctt
     3601 gggggatttt tgcacggaaa acaaattgcc accacaattt ttgagttcat gcgggttcca
     3661 aagtttgatc ccgaagaccc caaccacgtt cgcatatctg aaatttctct gcaagctcac
     3721 aagacgcgga ccaagaattt cagcacaaag ccgctgtcag aggaatacga aaaggaattg
     3781 gccgaactcg ttcgggcagt cgcatggacg cgccaaaccg gctctgcgtc gttcgtaaaa
     3841 ggcaaaccgt agtccatcga ctctcaaatg gggtcacagc agttccgatg gcccctaccc
     3901 cggacaactt atccgattgc cggtttctgc ctacattggc aaaaatagaa ctgccgcgtt
     3961 gccttgtgct tcccgctggt gcgaaaactc cagggtctcg gcaatcgagc aaggcgcggc
     4021 gttgccttac tctataagtg aagtgattaa gaattaaggg ggacaaagag ccatggatga
     4081 ggcggagatg caagcggagc ttgagcgcct gcgggcggag aatgcccaac tcaagagcaa
     4141 ggggaagggc ggcatcagtc tcaaggtgag cgagaagggc gcggtttccc tctatggcat
     4201 gggccgcttc ccggtcacgc tttataaaga gcagtggctg cgcctgctgg ccagcgcggc
     4261 tgaaatcgag gcattcatcc gtgaaaacga gccgcagttg aaaaccaagg aataatcccc
     4321 tgggccgcgc aagctgctca aggggtctca gcttttcccg atgccgcgcc cccggacaac
     4381 taacccgatt gcccgtttcc gccagcgtca catattcaaa aaaatagaaa aacgcctgtg
     4441 tgccgaccga acaacctggc ccgcgcgcga ttgattaccc accccttgag aacgcaatgc
     4501 cgcccctcgt gctgcaacag atgcgggcca ccagcttgaa ttgttgttgc cgatggtgca
     4561 tccagcggcg acgtgcgcgc cagaatcgcc cagcatcaag ccctgcggct agacctggca
     4621 aaacggctca agcgccattc cgctgggggt acatttgagg gtacgcagtc tggcgctaat
     4681 ttgcatgtaa ctgattaata gagacttgca tctttaatat gaatccctct cccccgccat
     4741 agagtcttgc tagagcgtcc cagaacatcc aattttcaaa tcaaaacaag ctgaaaacaa
     4801 gggagatatt gcctttaggc gtccatcggg gtactatgaa agccacattc ccgtgggggt
     4861 acttttgggg gtatgttgaa attcccctgg gggtatcttc ccaaacaggc gattggaggc
     4921 tctcccccgg tcacgcgagc gcgatttcta ctcgtcgagg ggccacttca gaatcagaac
     4981 gtggtcatcc atattcgagc gaaacgcctg gctcctctct cgaaccagcc catcggcatt
     5041 cggtctcatg acttacattg aatctgcctg ccacccagga atctccagtt atggctggca
     5101 gagaaagacg ggcaggtcgc gggcccccga gaaaactgag tggccgatgt tggtttctgg
     5161 caggaacgga atctttcaga taggaatgac tccgaaggcg acggcggcaa ttgtcatgac
     5221 gaccgatgcg gcgaaaaggt agggtatggc aaacttctgg tgatcgccca gttccacccc
     5281 ggcaagtccc accagcagaa acgttgccgg cgtcagtggg ctgatcggga agccggtggt
     5341 catctgcccc agaacagcgg cctgcgccat atgaaccggg gcgacgccca atactttgcc
     5401 tgcacccgcg aggacgggca ataagccaaa gtagaacgaa tccgggtcaa atatcaaact
     5461 gaggggcatt gaaaccagtc ccagtacaaa gggcatttgc cctgccattc gaacaggaac
     5521 atgcgaggcg actgatttcg ccattgccgt cagcatcccg gttcctccca gaatgccggt
     5581 gaaggccccg gcggcaaata gaactgccgc catcagcaga gcggcccggg catgggcatc
     5641 aatgcgctca gcctgttgtt ttaaatttgg atagttgacc gtaagcgcca gaaccgtgcc
     5701 gcacatgaat accgtcgccg gttccagccg cagaccaacc atggcgacaa tcagaccgat
     5761 tgtgaggatg agattgaacc agaagcagcg gggcctgcgc aactggagtt cctcctcggt
     5821 cagatgccgg ataaacgcct ctgtcgcctt tcctttttgg ttcaggccga gccgccgtgc
     5881 ctcgcgcgcg cccatccacc atgcaaccgc aaagacgtac accaggccta ccaactgcac
     5941 cggcgccagg ggattgaaga ttgcggtgat gggaatatga agggccgccg aagcgcggat
     6001 catgggcccg gtccatggca ggaagttcac acccgcggcc aatgcggcgg cacaggccag
     6061 aaccctccgg tccatcttca ggcgatcgaa aagtggcagc atgaccggga tggtgaccag
     6121 aaatgtgacc gcgcccgatc catccaggtg cgcaagcaag gcaagcagcg ccgttcccac
     6181 cacgatgcgc gatggagaga ggccgacagc acgcagaatt cgatcgatga tcggatcgag
     6241 cattcccgcg tcggtcatca ccccaaaata caggattgcg aagatgaaca tcgccgcaac
     6301 cggggcaaca ttttggattc catgaccgat gaagattgcg gttttgccgc cgaagccgcc
     6361 agccagagac gccagcaccg gaaaagtgat cagggcaacg atcggcgaca agcgccgcgt
     6421 gagaatcgtg accagcatca gcacgatagc ggcaaggccc aggatggcta gcatttggtt
     6481 atcggcgtga ctccgtttcg ccggttgagg aatcgcccgg ccgcacgctt ctcccttccg
     6541 gcgtcagcgt ggccc
//
LOCUS       DASSHJ010000278         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_591154, whole
            genome shotgun sequence.
ACCESSION   DASSHJ010000278 DASSHJ010000000
VERSION     DASSHJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591154"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..946)
                     /gene="asd"
                     /locus_tag="VFI43_10625"
     CDS             complement(44..946)
                     /gene="asd"
                     /locus_tag="VFI43_10625"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441436.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HET7062621.1"
                     /translation="MFSSSFSVLPQYLMPKQAMTVLAGHIANASAGLGTTLIIRWFIG
                     RYGVNMDEAVNPDIRSYRTFNDFFTRSLLAEARPIDTADYVCPADGTISQLGVISGDR
                     IFQAKGHNYSATALVGGDETLAGKFYGGNFATIYLSPRDYHRVHIPCKGRLLRMIHVP
                     GSLFSVNPGTVRGVPGLFARNERVISVFETDCGPFVMILVGATIVGSISTVWHGVVNP
                     PRSGETRDWRYDSSNIVLEKGDEMGQFQLGSTVIMLFPRNTLVFNPLWGPGRTVRFGE
                     MMGLKGGSSEGSTGDSARCSTPPA"
     gene            complement(1266..>2780)
                     /locus_tag="VFI43_10630"
     CDS             complement(1266..>2780)
                     /locus_tag="VFI43_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET7062622.1"
                     /translation="IRRVAIKVFGHLHALSLRFHLERQTGGVSRDIERGSRGISFLLN
                     FMLFNILPTFLEIGLVAAILLSRYDIWFSVIVLGTLVAYIALTLIVTEWRMIFRRTMN
                     DMDSKANTQAIDSLLNYETVKYFGNESWEARRYDEHMRNWETAAVRNQTSLALLNSGQ
                     SAIIAIGVTLLLTLAAEGVINGTMSLGDLVLINAFMLQLYMPLHFLGFVYREIKHSLA
                     DMEKMFDLLEQHEEIRDKPGAVDLIGDNAGICFEHVDFAYEPNRQILFDVTFKIPSGR
                     NVAVVGHSGSGKSTLARLLFRFYDVTSGRILINGQDIRDVTQKSLRAAIGIVPQDAVL
                     FNDSIYYNIAYGRPEATREEVIAAAKSAHLHEFIEKLPGGYQTTVGERGLKLSGGEKQ
                     RVAIARAILKNPSILIFDEATSALDSNSEKAIQAELKRIARNRTTLTIAHRLSTIVES
                     DEILVMDNGRIAERGKHRELLETSGLYARMWEAQQRERKAQLELTDGLKELATR"
BASE COUNT          645 a          773 c          729 g          633 t
ORIGIN      
        1 ctgagggcct gaccatcttg cttttcaact gactcctcct gtgctatgca ggaggagttg
       61 aacaccgagc cgaatctccc gtactccctt cactgcttcc tcccttcaag cccatcattt
      121 ctccgaagcg gaccgtgcgt ccgggccccc agagcgggtt gaaaaccaat gtgttcctgg
      181 ggaacagcat gattacggta gacccgagct gaaactgacc catttcatcc cccttttcaa
      241 gcacgatatt gctcgaatca taccgccaat cccgggtttc tccagaccgc ggcggattta
      301 ccacgccatg ccataccgtt gagatgctac cgacgatggt tgctcccacc agaatcatga
      361 caaatggccc gcagtcagtt tcaaaaactg agatcacccg ttcgttgcga gcgaagagcc
      421 cgggaacacc gcgaactgtt cccgggttga cggagaacag gctgcctgga acatgaatca
      481 tccggaggag cctacctttg cacgggatgt gcacccgatg gtaatccctt gggctcaggt
      541 agatggtggc gaaattacct ccataaaact ttcctgccag tgtctcgtcg cctcccacga
      601 gggcggtggc cgagtagttg tgccctttgg cctggaatat gcgatcgccc gagataaccc
      661 caagctgact tatcgttcca tccgccggac agacgtaatc cgctgtgtcg ataggccttg
      721 cctcggcaag cagagagcgc gtaaagaaat cgttgaacgt ccgatagctg cgaatatcag
      781 gatttaccgc ttcgtccata ttgacgccgt aacgcccgat aaaccagcgg atgatgaggg
      841 tggtcccaag gcccgcgctg gcattggcga tatggcctgc cagaaccgtc atggcctgct
      901 tgggcatcag atactggggc aatacggaaa aagatgaact gaacactcag aaggcctttt
      961 aaaaaacgcg attatagttc aagtcttgat acccggacga ggtagagctt acattcctgt
     1021 tcagcctgat gcttttaacg attttcggtc cgattttcac cgatatcacc ccttacatcc
     1081 gtgctctctt gtatgacaga agcaggtgaa aaaaaatcca tattgccttc gcttccaagc
     1141 agcatctcca caattgcatc aacccgagaa gaacagacga cacatttccg aagtgtatct
     1201 tgagacactt ggaacggcgg ctgccgaagc caggggaaat ggaaggactg cttgaaaacc
     1261 aagcgtcagc gggtcgcaag ttccttcagc ccatccgtca gttccagctg tgcttttctt
     1321 tcccgctgct gcgcttccca catgcgcgca tacaggccgc ttgtttccag taattcccga
     1381 tgctttcccc gctcggcaat gcgcccattg tccatgacga gaatctcgtc agactcgaca
     1441 atagtggaga gcctgtgtgc aattgtcagc gtagtgcggt tcctggcgat tcgctttagc
     1501 tctgcctgga tggctttttc ggagttcgag tccaatgccg aggtcgcttc gtcgaaaatc
     1561 aggatcgagg ggttcttgag aatggcgcgc gcaatcgcga cgcgctgctt ttcgccaccg
     1621 gatagtttca ggccgcgctc gccgacggtc gtctggtatc ctccaggaag tttttctata
     1681 aactcatgga gatgcgctga tttcgcagcg gcgatgactt cctcacgtgt tgcctccgga
     1741 cgcccatagg caatgttgta atagatactg tcattgaaaa gaacagcatc ctgggggacg
     1801 atacctatcg cagcccgcaa gcttttttgc gtgacgtccc ggatgtcctg tccgtttatc
     1861 agaatgcgtc ccgacgtaac gtcgtagaag cggaacagca accgtgccag ggtcgatttg
     1921 ccggaaccgc tgtgtcccac gaccgccacg ttcctgccgg atggaatctt gaaggtgaca
     1981 tcaaagagaa tttgtcggtt aggctcgtag gcgaaatcga cgtgctcgaa gcaaatcccc
     2041 gcgttgtcgc ctatgagatc caccgctcca ggcttatccc tgatttcttc gtgctgctcc
     2101 agcaaatcga acatcttttc catgtccgcg agggagtgct tgatttcgcg gtaaacgaaa
     2161 cctagaaaat gcagcggcat gtagagttgc aacatgaaag cattgatgag caccaggtcg
     2221 cccaacgaca tcgttccgtt aatgacgcct tcagcagcca gagtcagcag cagggtaacg
     2281 ccgattgcga taatcgcgct ttgccccgag ttaagaaggg caagcgaagt ctggttgcgc
     2341 accgcagcgg tctcccagtt ccgcatatgc tcgtcgtagc ggcgcgcttc ccaggactcg
     2401 ttgccaaagt acttgacggt ttcatagttc agcagactgt cgatcgcctg ggtgttggct
     2461 ttggagtcca tgtcgttcat cgtgcgacgg aaaatcatgc gccattcggt gacgatcagt
     2521 gtaagtgcga tataagcaac caaggtaccc aggacaatga cggagaacca gatgtcgtat
     2581 ctcgacaaca gtatcgcggc gaccagcccg atttccagga aggttggcag gatgttgaac
     2641 aacatgaaat taagcagaaa ggaaatgcct cgcgaaccac gctcgatatc gcgagaaact
     2701 ccccccgttt gccgttccag gtggaagcgt aacgacagcg cgtggaggtg gccgaaaacc
     2761 ttgatggcaa cgcgccggat
//
LOCUS       DASSHJ010000279         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrosospira sp. isolate SMAG_U1056 k141_1041371,
            whole genome shotgun sequence.
ACCESSION   DASSHJ010000279 DASSHJ010000000
VERSION     DASSHJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963221
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosospira sp. (soil metagenome)
  ORGANISM  Nitrosospira sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Nitrosomonadaceae; Nitrosospira.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,101
            Genes (coding)                    :: 2,087
            CDSs (with protein)               :: 2,087
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 7 of 14
            Pseudo Genes (incomplete)         :: 10 of 14
            Pseudo Genes (internal stop)      :: 3 of 14
            Pseudo Genes (multiple problems)  :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Nitrosospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041371"
                     /isolate="SMAG_U1056"
                     /isolation_source="soil"
                     /db_xref="taxon:38080"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2082
                     /locus_tag="VFI43_10635"
     CDS             <1..2082
                     /locus_tag="VFI43_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7062623.1"
                     /translation="VDDATVMIENIDTHLEMGKELDEAIIDAANQIVIPTLLATLCIC
                     IVWLPLFTLSGVSGYLFKPMALAVIFAMLASFILSRTLVPTMAKYLIKHPHAEGHGEG
                     HTEGHGKEGGHSEEKPAGKFAGFQQKLKSPFAFFTRAQEGFERSFLRFCGRYGGLLER
                     TVENRGRFATVMLAFALVSLILFYFNGRDFFPEVRSGTVQMHLRGPLGTRMESINRIA
                     TLVSNDIQRMLPGQVEGIVSNCGLPVGPHNLAFIPTPTIGTQDCDLTITLFNDKSAVW
                     DIRKILHDGLKARYPGTEFTFQPADLIAKILNFGAPSPITVQVNGMDIYANYEYARKL
                     QGRLRKIAGSSDVVIQQTMRTPTMFIEGQRTYGLGVDRSESDIALNMLITTNGTQQID
                     QKYWLDLKTGMSYQINIYQPQPQVSSINELNRIPVKPVGEGGLDQNYQLLGNLTKKSP
                     VGTPGLVTHRGIMPLIEVYVSAEGRGLGDVLDEVKKIVAEMDEEVPRGSDVQIAGQAA
                     NMVTTYGELLIGLVAAVVLIYLMLVINFQSWLIPFIIITALPGALAGIAWALFLTQTN
                     ISVPALTGAIMAMGTATANSILVVSYALERIEKHGDPLLAAVEAGKARIRPVLMTAAA
                     MIMGMLPMAMGGSTNAPLGRAVIGGLTFATIYTLFFVPAVYAIMYTRSVPKDKESVPG
                     DKEIVPQLGNS"
     gene            2084..3349
                     /locus_tag="VFI43_10640"
     CDS             2084..3349
                     /locus_tag="VFI43_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7062624.1"
                     /translation="MKKLGQKTTMIFAIVLGVVYMGYRVIESRADMASLTEQAAENDV
                     PTVAIVHAEPLPAKETIQLPGTIEAWFQAPIYAQVSGYVKMWHKDWGARVQKGDILAE
                     INAPALDAQYRQAKADLASEIALYDLAELTANRYIALRKNQAVSEQAISVKVAEAKAQ
                     LAKVNAAKQNVRNYEALINFKTIVAPYDGVVINRAINVGDYINKEGTISTPQGDIRNL
                     YTVADIRRLRLFVSVPERFGALMHDGLKADLTVPQFPNRHFTAEFLTTARGFDVSTRT
                     VVTQFVIENEEKDLWPGSYASVNLTVDVQNKHLSIPSSSLVFDEMGTRVAVVDDDNIV
                     HFKPIKISRILDATVELTEGVSTEDRIINNPSAAILDGDKVAIVEPAPGYDLINKPKE
                     KVSASSKPDSHAEMPDTKLKQASQGSHAQ"
BASE COUNT          952 a          962 c         1003 g          900 t
ORIGIN      
        1 gtcgacgacg cgacagtgat gatcgagaac atcgatactc acctggaaat gggcaaggaa
       61 ctcgatgagg caatcatcga tgcggcaaac cagatcgtta ttccaacgct gctcgccaca
      121 ttatgcatat gcattgtctg gttgccgctg ttcacgcttt ccggcgtatc gggctatctg
      181 ttcaagccaa tggcgctggc ggtgatattt gccatgctgg catccttcat cctgtcacgt
      241 acgcttgtgc cgaccatggc gaaatatctt atcaagcatc ctcacgcaga agggcacgga
      301 gaaggacaca cagaaggaca cggaaaggaa ggcggccata gcgaggaaaa gccggcggga
      361 aagtttgcag gttttcagca gaaattaaag agcccctttg cgttttttac ccgtgctcag
      421 gaggggttcg agagaagctt tcttcgattc tgtggcaggt atggggggct gctcgaacga
      481 acagttgaaa atcgcggccg gttcgcaacg gtcatgctgg ctttcgccct tgtctcttta
      541 attcttttct acttcaacgg ccgcgatttt ttcccggagg tcaggtcagg aaccgtacaa
      601 atgcatttgc gtggacctct tggcacgcgg atggaatcca taaatcgcat cgccactctc
      661 gtttcgaatg atattcagcg aatgcttccc gggcaggttg aaggcatcgt cagcaattgc
      721 ggcttacccg tcgggccgca taatctggcg tttattccga cgcccacgat cggaacgcag
      781 gactgcgatc tgacgatcac cctgttcaac gataaatctg cggtgtggga tatccggaaa
      841 attctccatg acggtctgaa agcacgttat ccgggaactg aattcacatt tcagccagcc
      901 gatctgatag caaaaattct gaatttcggt gcgccttctc cgattactgt tcaggttaac
      961 gggatggata tatacgcaaa ctacgaatac gcacgcaaat tgcaaggcag gctgcgtaaa
     1021 atcgctggtt cctccgacgt ggtgatccag caaaccatgc gcacgcccac catgtttatt
     1081 gaaggccaaa ggacctatgg actcggtgtt gataggagcg aatcggatat tgccctgaat
     1141 atgctgataa cgactaacgg aacccagcag atcgaccaga aatactggct ggatctgaag
     1201 acgggcatgt cataccagat caacatctat cagcctcaac cgcaagtctc gagcatcaat
     1261 gagttgaaca ggattcctgt caagccagtg ggggagggag gcctggatca gaattatcag
     1321 cttctgggca atctgacgaa gaaatcgccg gtaggcaccc ccggcctggt cacccacagg
     1381 ggcatcatgc cgttgattga agtgtatgta tccgccgaag ggcgggggct gggcgacgtg
     1441 ctggatgagg tcaagaaaat cgtcgcggag atggacgaag aagtaccccg cggctccgat
     1501 gtccaaatcg ccggccaggc cgcgaatatg gttacgacct atggcgaact acttatcggc
     1561 cttgtagcgg ctgtggtgct gatctatctc atgcttgtca tcaattttca atcatggctg
     1621 attcccttca tcatcattac agcactgccc ggcgctttgg ccggcattgc ctgggccctg
     1681 ttcctgaccc agacgaatat ttcggttccg gccctgacag gcgccatcat ggccatggga
     1741 acggccacgg caaattccat acttgtcgtg tcctatgcgt tggagcgtat cgaaaagcac
     1801 ggcgaccctc tgctagccgc cgttgaggcc ggaaaagccc ggattcgtcc tgttctcatg
     1861 accgcagcag ccatgatcat gggtatgctt ccgatggcga tgggaggatc caccaatgcg
     1921 ccgctgggcc gtgcagttat tggcggcttg acgtttgcaa ccatatatac cctattcttt
     1981 gtcccggctg tgtatgccat catgtataca cgaagcgttc ctaaagacaa ggaaagcgtt
     2041 cctggcgaca aggaaatcgt tcctcaactc ggaaattcct gatatgaaaa aactaggcca
     2101 aaaaactacg atgatttttg ccatcgttct cggtgttgtc tatatgggct accgagtgat
     2161 tgaatccagg gccgatatgg cttctttaac agaacaagca gcggaaaatg acgttccgac
     2221 ggttgccatc gtccacgccg agccgctgcc tgccaaggaa accattcagc ttccaggtac
     2281 cattgaggcc tggtttcagg cgccaatcta tgcccaggta tccggttacg tcaaaatgtg
     2341 gcataaggat tggggggcga gagtccaaaa aggcgatatc cttgccgaaa tcaacgcccc
     2401 cgcgctcgat gcccaatata ggcaagccaa ggcggatttg gcgtcggaaa tagcccttta
     2461 tgatctcgct gaacttactg ccaaccgata tatagccttg cgcaaaaacc aggccgtatc
     2521 cgagcaggcg atttcggtaa aagtggcgga ggcaaaagcg caactggcaa aggttaatgc
     2581 ggccaagcaa aatgtccgta actatgaagc attgattaac tttaaaacaa ttgtggcgcc
     2641 ctacgacggg gtggtcatta accgtgccat caatgtggga gactacatca ataaggaagg
     2701 tactatcagt acgccccaag gtgacattcg caatctttat actgtcgcgg acatcaggcg
     2761 gttgcgcttg tttgtaagcg tgcctgagcg gtttggcgca cttatgcatg atggcctgaa
     2821 agccgacctg acggtgccgc aatttcccaa taggcatttc actgccgagt ttctgaccac
     2881 tgctcgtggc ttcgatgtga gtacacgtac ggtggtcacg caattcgtga ttgaaaatga
     2941 agagaaggat ctgtggcccg gctcctatgc atcagtcaac ctgaccgtgg atgttcagaa
     3001 taaacacctc tctatcccat ccagctcact cgtatttgat gagatgggga cgcgagtggc
     3061 cgtggtggat gacgacaaca tcgttcactt caagccgatc aaaataagca gaattctcga
     3121 cgctactgtg gaattaaccg agggtgtctc aacagaggat cggattataa acaatcccag
     3181 cgccgccata cttgatggtg acaaggtagc cattgtagag cctgcaccgg ggtatgacct
     3241 gatcaataaa ccaaaagaga aggtctcggc gtcatcgaaa ccagactcgc atgcagaaat
     3301 gccagatacg aagttaaaac aagcgtcaca gggatcacac gcacaatgac tcatttgact
     3361 atgattgact tgcgacgtaa aaaaagaatg tatatttcaa acctctgtcg tctcggcgcg
     3421 agatttggga gttttttatt cctggcaacc agcttggcag cttgcagcag tcttcccttt
     3481 cctgcgttca atctgtcacc cacttataaa tcgcctgaat ttgttgttcc ggattcttgg
     3541 gagggaacga gttcttttgt caaggctagg ccgtcggatg acgcgctgcg gcccgactgg
     3601 tggaagctat atgacgatcc ggttctaaac agccttgtag agcaggggat gttagccaat
     3661 ccagacttgc aggcagcagc tgaacggttc gttcaagcgc gcgatataat gatgaaggcg
     3721 cggtctcgat attttcccca acttggcttt gatttcggtg cgaataataa taggcaatcc
     3781 gatcatagcc tctttcgtgc tcctggagaa cctaata
//
